PDE3B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Gene Symbol	PDE3B
Entrez Gene ID	5140
Full Name	phosphodiesterase 3B
Synonyms	HcGIP1,cGIPDE1
General protein information	Preferred Names phosphodiesterase 3B Names cGMP-inhibited 3',5'-cyclic phosphodiesterase B CGI-PDE B CGIP1 cyclic GMP-inhibited phosphodiesterase B cyclic nucleotide phosphodiesterase phosphodiesterase 3B, cGMP-inhibited
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11p15.2
Disorder MIM:	602047

mRNA and Protein(s)

mRNA	Protein	Name
NM_001363569.1	NP_001350498.1	cGMP-inhibited 3',5'-cyclic phosphodiesterase B isoform 3
NM_001363570.1	NP_001350499.1	cGMP-inhibited 3',5'-cyclic phosphodiesterase B isoform 1
XM_006718249.3	XP_006718312.1	cGMP-inhibited 3',5'-cyclic phosphodiesterase B isoform X3
NM_000922.3	NP_000913.2	cGMP-inhibited 3',5'-cyclic phosphodiesterase B
XM_017017911.1	XP_016873400.1	cGMP-inhibited 3',5'-cyclic phosphodiesterase B isoform X1
XM_017017911.2	XP_016873400.1	cGMP-inhibited 3',5'-cyclic phosphodiesterase B isoform X1
XM_017017912.1	XP_016873401.1	cGMP-inhibited 3',5'-cyclic phosphodiesterase B isoform X2
NM_000922.4	NP_000913.2	cGMP-inhibited 3',5'-cyclic phosphodiesterase B isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	PDE3B	NP_000913.2
Pan troglodytes (chimpanzee)	PDE3B	XP_003312976.1
	PDE3B	XP_001092891.1
Canis lupus familiaris (dog)	PDE3B	XP_854529.2
Bos taurus (cattle)	PDE3B	XP_002693091.1
Mus musculus (house mouse)	Pde3b	NP_035185.2
Rattus norvegicus (Norway rat)	Pde3b	NP_058925.1
Gallus gallus (chicken)	PDE3B	NP_001026353.1
Danio rerio (zebrafish)	pde3b	XP_691883.4

Gene Ontology
Bibliography

Related articles in PubMed

Clinical effects of phosphodiesterase 3A mutations in inherited hypertension with brachydactyly.
Toka O, Tank J, Sch?chterle C, Aydin A, Maass PG, Elitok S, Bartels-Klein E, Hollfinger I, Lindschau C, Mai K, Boschmann M, Rahn G, Movsesian MA, M?ller T, Doescher A, Gnoth S, M?hl A, Toka HR, Wefeld-Neuenfeld Y, Utz W, T?pper A, Jordan J, Schulz-Menger J, Klussmann E, B?hring S, Luft FC
Hypertension (Dallas, Tex. : 1979)66(4)800-8(2015 Oct)

Genome-wide association study of vitamin D levels in children: replication in the Western Australian Pregnancy Cohort (Raine) study.
Anderson D, Holt BJ, Pennell CE, Holt PG, Hart PH, Blackwell JM
Genes and immunity15(8)578-83(2014 Dec)

Novel loci for adiponectin levels and their influence on type 2 diabetes and metabolic traits: a multi-ethnic meta-analysis of 45,891 individuals.
Dastani Z, Hivert MF, Timpson N, Perry JR, Yuan X, Scott RA, Henneman P, Heid IM, Kizer JR, Lyytik?inen LP, Fuchsberger C, Tanaka T, Morris AP, Small K, Isaacs A, Beekman M, Coassin S, Lohman K, Qi L, Kanoni S, Pankow JS, Uh HW, Wu Y, Bidulescu A, Rasmussen-Torvik LJ, Greenwood CM, Ladouceur M, Grimsby J, Manning AK, Liu CT, Kooner J, Mooser VE, Vollenweider P, Kapur KA, Chambers J, Wareham NJ, Langenberg C, Frants R, Willems-Vandijk K, Oostra BA, Willems SM, Lamina C, Winkler TW, Psaty BM, Tracy RP, Brody J, Chen I, Viikari J, K?h?nen M, Pramstaller PP, Evans DM, St Pourcain B, Sattar N, Wood AR, Bandinelli S, Carlson OD, Egan JM, B?hringer S, van Heemst D, Kedenko L, Kristiansson K, Nuotio ML, Loo BM, Harris T, Garcia M, Kanaya A, Haun M, Klopp N, Wichmann HE, Deloukas P, Katsareli E, Couper DJ, Duncan BB, Kloppenburg M, Adair LS, Borja JB, , , , , Wilson JG, Musani S, Guo X, Johnson T, Semple R, Teslovich TM, Allison MA, Redline S, Buxbaum SG, Mohlke KL, Meulenbelt I, Ballantyne CM, Dedoussis GV, Hu FB, Liu Y, Paulweber B, Spector TD, Slagboom PE, Ferrucci L, Jula A, Perola M, Raitakari O, Florez JC, Salomaa V, Eriksson JG, Frayling TM, Hicks AA, Lehtim?ki T, Smith GD, Siscovick DS, Kronenberg F, van Duijn C, Loos RJ, Waterworth DM, Meigs JB, Dupuis J, Richards JB, Voight BF, Scott LJ, Steinthorsdottir V, Dina C, Welch RP, Zeggini E, Huth C, Aulchenko YS, Thorleifsson G, McCulloch LJ, Ferreira T, Grallert H, Amin N, Wu G, Willer CJ, Raychaudhuri S, McCarroll SA, Hofmann OM, Segr? AV, van Hoek M, Navarro P, Ardlie K, Balkau B, Benediktsson R, Bennett AJ, Blagieva R, Boerwinkle E, Bonnycastle LL, Bostr?m KB, Bravenboer B, Bumpstead S, Burtt NP, Charpentier G, Chines PS, Cornelis M, Crawford G, Doney AS, Elliott KS, Elliott AL, Erdos MR, Fox CS, Franklin CS, Ganser M, Gieger C, Grarup N, Green T, Griffin S, Groves CJ, Guiducci C, Hadjadj S, Hassanali N, Herder C, Isomaa B, Jackson AU, Johnson PR, J?rgensen T, Kao WH, Kong A, Kraft P, Kuusisto J, Lauritzen T, Li M, Lieverse A, Lindgren CM, Lyssenko V, Marre M, Meitinger T, Midthjell K, Morken MA, Narisu N, Nilsson P, Owen KR, Payne F, Petersen AK, Platou C, Proen?a C, Prokopenko I, Rathmann W, Rayner NW, Robertson NR, Rocheleau G, Roden M, Sampson MJ, Saxena R, Shields BM, Shrader P, Sigurdsson G, Spars? T, Strassburger K, Stringham HM, Sun Q, Swift AJ, Thorand B, Tichet J, Tuomi T, van Dam RM, van Haeften TW, van Herpt T, van Vliet-Ostaptchouk JV, Walters GB, Weedon MN, Wijmenga C, Witteman J, Bergman RN, Cauchi S, Collins FS, Gloyn AL, Gyllensten U, Hansen T, Hide WA, Hitman GA, Hofman A, Hunter DJ, Hveem K, Laakso M, Morris AD, Palmer CN, Rudan I, Sijbrands E, Stein LD, Tuomilehto J, Uitterlinden A, Walker M, Watanabe RM, Abecasis GR, Boehm BO, Campbell H, Daly MJ, Hattersley AT, Pedersen O, Barroso I, Groop L, Sladek R, Thorsteinsdottir U, Wilson JF, Illig T, Froguel P, van Duijn CM, Stefansson K, Altshuler D, Boehnke M, McCarthy MI, Soranzo N, Wheeler E, Glazer NL, Bouatia-Naji N, M?gi R, Randall J, Elliott P, Rybin D, Dehghan A, Hottenga JJ, Song K, Goel A, Lajunen T, Doney A, Cavalcanti-Proen?a C, Kumari M, Timpson NJ, Zabena C, Ingelsson E, An P, O'Connell J, Luan J, Elliott A, McCarroll SA, Roccasecca RM, Pattou F, Sethupathy P, Ariyurek Y, Barter P, Beilby JP, Ben-Shlomo Y, Bergmann S, Bochud M, Bonnefond A, Borch-Johnsen K, B?ttcher Y, Brunner E, Bumpstead SJ, Chen YD, Chines P, Clarke R, Coin LJ, Cooper MN, Crisponi L, Day IN, de Geus EJ, Delplanque J, Fedson AC, Fischer-Rosinsky A, Forouhi NG, Franzosi MG, Galan P, Goodarzi MO, Graessler J, Grundy S, Gwilliam R, Hallmans G, Hammond N, Han X, Hartikainen AL, Hayward C, Heath SC, Hercberg S, Hillman DR, Hingorani AD, Hui J, Hung J, Kaakinen M, Kaprio J, Kesaniemi YA, Kivimaki M, Knight B, Koskinen S, Kovacs P, Kyvik KO, Lathrop GM, Lawlor DA, Le Bacquer O, Lecoeur C, Li Y, Mahley R, Mangino M, Mart?nez-Larrad MT, McAteer JB, McPherson R, Meisinger C, Melzer D, Meyre D, Mitchell BD, Mukherjee S, Naitza S, Neville MJ, Orr? M, Pakyz R, Paolisso G, Pattaro C, Pearson D, Peden JF, Pedersen NL, Pfeiffer AF, Pichler I, Polasek O, Posthuma D, Potter SC, Pouta A, Province MA, Rayner NW, Rice K, Ripatti S, Rivadeneira F, Rolandsson O, Sandbaek A, Sandhu M, Sanna S, Sayer AA, Scheet P, Seedorf U, Sharp SJ, Shields B, Sigur?sson G, Sijbrands EJ, Silveira A, Simpson L, Singleton A, Smith NL, Sovio U, Swift A, Syddall H, Syv?nen AC, T?njes A, Uitterlinden AG, van Dijk KW, Varma D, Visvikis-Siest S, Vitart V, Vogelzangs N, Waeber G, Wagner PJ, Walley A, Ward KL, Watkins H, Wild SH, Willemsen G, Witteman JC, Yarnell JW, Zelenika D, Zethelius B, Zhai G, Zhao JH, Zillikens MC, , , , Borecki IB, Meneton P, Magnusson PK, Nathan DM, Williams GH, Silander K, Bornstein SR, Schwarz P, Spranger J, Karpe F, Shuldiner AR, Cooper C, Serrano-R?os M, Lind L, Palmer LJ, Hu FB 1st, Franks PW, Ebrahim S, Marmot M, Kao WH, Pramstaller PP, Wright AF, Stumvoll M, Hamsten A, , Buchanan TA, Valle TT, Rotter JI, Penninx BW, Boomsma DI, Cao A, Scuteri A, Schlessinger D, Uda M, Ruokonen A, Jarvelin MR, Peltonen L, Mooser V, Sladek R, , , Musunuru K, Smith AV, Edmondson AC, Stylianou IM, Koseki M, Pirruccello JP, Chasman DI, Johansen CT, Fouchier SW, Peloso GM, Barbalic M, Ricketts SL, Bis JC, Feitosa MF, Orho-Melander M, Melander O, Li X, Li M, Cho YS, Go MJ, Kim YJ, Lee JY, Park T, Kim K, Sim X, Ong RT, Croteau-Chonka DC, Lange LA, Smith JD, Ziegler A, Zhang W, Zee RY, Whitfield JB, Thompson JR, Surakka I, Spector TD, Smit JH, Sinisalo J, Scott J, Saharinen J, Sabatti C, Rose LM, Roberts R, Rieder M, Parker AN, Pare G, O'Donnell CJ, Nieminen MS, Nickerson DA, Montgomery GW, McArdle W, Masson D, Martin NG, Marroni F, Lucas G, Luben R, Lokki ML, Lettre G, Launer LJ, Lakatta EG, Laaksonen R, Kyvik KO, K?nig IR, Khaw KT, Kaplan LM, Johansson ?, Janssens AC, Igl W, Hovingh GK, Hengstenberg C, Havulinna AS, Hastie ND, Harris TB, Haritunians T, Hall AS, Groop LC, Gonzalez E, Freimer NB, Erdmann J, Ejebe KG, D?ring A, Dominiczak AF, Demissie S, Deloukas P, de Faire U, Crawford G, Chen YD, Caulfield MJ, Boekholdt SM, Assimes TL, Quertermous T, Seielstad M, Wong TY, Tai ES, Feranil AB, Kuzawa CW, Taylor HA Jr, Gabriel SB, Holm H, Gudnason V, Krauss RM, Ordovas JM, Munroe PB, Kooner JS, Tall AR, Hegele RA, Kastelein JJ, Schadt EE, Strachan DP, Reilly MP, Samani NJ, Schunkert H, Cupples LA, Sandhu MS, Ridker PM, Rader DJ, Kathiresan S
PLoS genetics8(3)e1002607(2012)

From PDE3B to the regulation of energy homeostasis.
Degerman E, Ahmad F, Chung YW, Guirguis E, Omar B, Stenson L, Manganiello V
Current opinion in pharmacology11(6)676-82(2011 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our results shed more light on phosphodiesterase 3A activation and could be relevant to the treatment of severe hypertension in the general population.
Title: Clinical effects of phosphodiesterase 3A mutations in inherited hypertension with brachydactyly.

Data indicate that genome-wide significant associations were found both at age 6 and 14 with single nucleotide polymorphisms (SNPs) on chromosome 11p15 in phosphodiesterase 3B, cGMP-inhibited protein PDE3B/cytochrome P-450 CYP2R1 genes.
Title: Genome-wide association study of vitamin D levels in children: replication in the Western Australian Pregnancy Cohort (Raine) study.

PDE3B appears to be an important actor in regulation of energy metabolism and energy intake. Is inhibition of PDE3B a possible target for treatment of obesity or type 2 diabetes mellitus? [REVIEW]
Title: From PDE3B to the regulation of energy homeostasis.

Rescue of low O(2)-induced ATP release from erythrocytes of type 2 diabetics by cilostazol restored the ability of erythrocytes to participate in the regulation of perfusion distribution in skeletal muscle.
Title: A selective phosphodiesterase 3 inhibitor rescues low PO2-induced ATP release from erythrocytes of humans with type 2 diabetes: implication for vascular control.

PDE3B and the anti-lipolytic action of insulin may have a role in increased S-nitrosylation of adipose tissue in obesity
Title: Increased adipocyte S-nitrosylation targets anti-lipolytic action of insulin: relevance to adipose tissue dysfunction in obesity.

The following PDE3B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDE3B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu12511	NM_000922.4 Latest version!	Homo sapiens phosphodiesterase 3B (PDE3B), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu106784	NM_001363569.1 Latest version!	Homo sapiens phosphodiesterase 3B (PDE3B), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu107571	NM_001363570.1 Latest version!	Homo sapiens phosphodiesterase 3B (PDE3B), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu44898	XM_006718249.3 Latest version!	Homo sapiens phosphodiesterase 3B (PDE3B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu92310	XM_017017911.2 Latest version!	Homo sapiens phosphodiesterase 3B (PDE3B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OHu92311	XM_017017912.1 Latest version!	Homo sapiens phosphodiesterase 3B (PDE3B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
View Old Accession Versions >>
OHu92310	XM_017017911.1	Homo sapiens phosphodiesterase 3B (PDE3B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OHu12511	NM_000922.3	Homo sapiens phosphodiesterase 3B (PDE3B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PDE3B ( NM_000922.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000913.2 Homo sapiens phosphodiesterase 3B (PDE3B), mRNA.

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CloneID	OHu12511
Clone ID Related Accession (Same CDS sequence)	NM_000922.3 , NM_000922.4
Accession Version	NM_000922.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC150307.1, BC136565.1 and AC090835.7. On Jan 9, 2009 this sequence version replaced NM_000922.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC150307.1, SRR1660805.52394.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGAGGG ACGAGCGAGA CGCCAAAGCC ATGCGGTCCC TGCAGCCGCC GGATGGGGCC 
GGCTCGCCCC CCGAGAGTCT GAGGAACGGC TACGTGAAGA GCTGCGTGAG CCCCTTGCGG 
CAGGACCCTC CGCGCGGCTT CTTCTTCCAC CTCTGCCGCT TCTGCAACGT GGAGCTGCGG 
CCGCCGCCGG CCTCTCCCCA GCAGCCGCGG CGCTGCTCCC CCTTCTGCCG GGCGCGCCTC 
TCGCTGGGCG CCCTGGCTGC CTTTGTCCTC GCCCTGCTGC TGGGCGCGGA ACCCGAGAGC 
TGGGCTGCCG GGGCCGCCTG GCTGCGGACG CTGCTGAGCG TGTGTTCGCA CAGCTTGAGC 
CCCCTCTTCA GCATCGCCTG TGCCTTCTTC TTCCTCACCT GCTTCCTCAC CCGGACCAAG 
CGGGGACCCG GCCCGGGCCG GAGCTGCGGC TCCTGGTGGC TGCTGGCGCT GCCCGCCTGC 
TGTTACCTGG GGGACTTCTT GGTGTGGCAG TGGTGGTCTT GGCCTTGGGG GGATGGCGAC 
GCAGGGTCCG CGGCCCCGCA CACGCCCCCG GAGGCGGCAG CGGGCAGGTT GCTGCTGGTG 
CTGAGCTGCG TAGGGCTGCT GCTGACGCTC GCGCACCCGC TGCGGCTCCG GCACTGCGTT 
CTGGTGCTGC TCCTGGCCAG CTTCGTCTGG TGGGTCTCCT TCACCAGCCT CGGGTCGCTG 
CCCTCCGCCC TCAGGCCGCT GCTCTCCGGC CTGGTGGGGG GCGCTGGCTG CCTGCTGGCC 
CTGGGGTTGG ATCACTTCTT TCAAATCAGG GAAGCGCCTC TTCATCCTCG ACTGTCCAGT 
GCCGCCGAAG AAAAAGTGCC TGTGATCCGA CCCCGGAGGA GGTCCAGCTG CGTGTCGTTA 
GGAGAAACTG CAGCCAGTTA CTATGGCAGT TGCAAAATAT TCAGGAGACC GTCGTTGCCT 
TGTATTTCCA GAGAACAGAT GATTCTTTGG GATTGGGACT TAAAACAATG GTATAAGCCT 
CATTATCAAA ATTCTGGAGG TGGAAATGGA GTTGATCTTT CAGTGCTAAA TGAGGCTCGC 
AATATGGTGT CAGATCTTCT GACTGATCCA AGCCTTCCAC CACAAGTCAT TTCCTCTCTA 
CGGAGTATTA GTAGCTTAAT GGGTGCTTTC TCAGGTTCCT GTAGGCCAAA GATTAATCCT 
CTCACACCAT TTCCTGGATT TTACCCCTGT TCTGAAATAG AGGACCCAGC TGAGAAAGGG 
GATAGAAAAC TTAACAAGGG ACTAAATAGG AATAGTTTGC CAACTCCACA GCTGAGGAGA 
AGCTCAGGAA CTTCAGGATT GCTACCTGTT GAACAGTCTT CAAGGTGGGA TCGTAATAAT 
GGCAAAAGAC CTCACCAAGA ATTTGGCATT TCAAGTCAAG GATGCTATCT AAATGGGCCT 
TTTAATTCAA ATCTACTGAC TATCCCGAAG CAAAGGTCAT CTTCTGTATC ACTGACTCAC 
CATGTAGGTC TCAGAAGAGC TGGTGTTTTG TCCAGTCTGA GTCCTGTGAA TTCTTCCAAC 
CATGGACCAG TGTCTACTGG CTCTCTAACT AATCGATCAC CCATAGAATT TCCTGATACT 
GCTGATTTTC TTAATAAGCC AAGCGTTATC TTGCAGAGAT CTCTGGGCAA TGCACCTAAT 
ACTCCAGATT TTTATCAGCA ACTTAGAAAT TCTGATAGCA ATCTGTGTAA CAGCTGTGGA 
CATCAAATGC TGAAATATGT TTCAACATCT GAATCAGATG GTACAGATTG CTGCAGTGGA 
AAATCAGGTG AAGAAGAAAA CATTTTCTCG AAAGAATCAT TCAAACTTAT GGAAACTCAA 
CAAGAAGAGG AAACAGAGAA GAAAGACAGC AGAAAATTAT TTCAGGAAGG TGATAAGTGG 
CTAACAGAAG AGGCACAGAG TGAACAGCAA ACAAATATTG AACAGGAAGT ATCACTGGAC 
CTGATTTTAG TAGAAGAGTA TGACTCATTA ATAGAAAAGA TGAGCAACTG GAATTTTCCA 
ATTTTTGAAC TTGTAGAAAA GATGGGAGAG AAATCAGGAA GGATTCTCAG TCAGGTTATG 
TATACCTTAT TTCAAGACAC TGGTTTATTG GAAATATTTA AAATTCCCAC TCAACAATTT 
ATGAACTATT TTCGTGCATT AGAAAATGGC TATCGAGACA TTCCTTATCA CAATCGTATA 
CATGCCACAG ATGTGCTACA TGCAGTTTGG TATCTGACAA CACGGCCAGT TCCTGGCTTA 
CAGCAGATCC ACAATGGTTG TGGAACAGGA AATGAAACAG ATTCTGATGG TAGAATTAAC 
CATGGGCGAA TTGCTTATAT TTCTTCGAAG AGCTGCTCTA ATCCTGATGA GAGTTATGGC 
TGCCTGTCTT CAAACATTCC TGCATTAGAA TTGATGGCTC TATACGTGGC AGCTGCCATG 
CATGATTATG ATCACCCAGG GAGGACAAAT GCATTTCTAG TGGCTACAAA TGCCCCTCAG 
GCAGTTTTAT ACAATGACAG ATCTGTTCTG GAAAATCATC ATGCTGCGTC AGCTTGGAAT 
CTATATCTTT CTCGCCCAGA ATACAACTTC CTTCTTCATC TTGATCATGT GGAATTCAAG 
CGCTTTCGTT TTTTAGTCAT TGAAGCAATC CTTGCTACGG ATCTTAAAAA GCATTTTGAT 
TTTCTCGCAG AATTCAATGC CAAGGCAAAT GATGTAAATA GTAATGGCAT AGAATGGAGT 
AATGAAAATG ATCGCCTCTT GGTATGCCAG GTGTGCATCA AACTGGCAGA TATAAATGGC 
CCAGCAAAAG TTCGAGACTT GCATTTGAAA TGGACAGAAG GCATTGTCAA TGAATTTTAT 
GAGCAGGGAG ATGAAGAAGC AAATCTTGGT CTGCCCATCA GTCCATTCAT GGATCGTTCT 
TCTCCTCAAC TAGCAAAACT CCAAGAATCT TTTATCACCC ACATAGTGGG TCCCCTGTGT 
AACTCCTATG ATGCTGCTGG TTTGCTACCA GGTCAGTGGT TAGAAGCAGA AGAGGATAAT 
GATACTGAAA GTGGTGATGA TGAAGACGGT GAAGAATTAG ATACAGAAGA TGAAGAAATG 
GAAAACAATC TAAATCCAAA ACCACCAAGA AGGAAAAGCA GACGGCGAAT ATTTTGTCAG 
CTAATGCACC ACCTCACTGA AAACCACAAG ATATGGAAGG AAATCGTAGA GGAAGAAGAA 
AAATGTAAAG CTGATGGGAA TAAACTGCAG GTGGAGAATT CCTCCTTACC TCAAGCAGAT 
GAGATTCAGG TAATTGAAGA GGCAGATGAA GAGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000913.2
CDS	354..3692
Translation	MRRDERDAKAMRSLQPPDGAGSPPESLRNGYVKSCVSPLRQDPPRGFFFHLCRFCNVELRPPPASPQQPRRCSPFCRARLSLGALAAFVLALLLGAEPESWAAGAAWLRTLLSVCSHSLSPLFSIACAFFFLTCFLTRTKRGPGPGRSCGSWWLLALPACCYLGDFLVWQWWSWPWGDGDAGSAAPHTPPEAAAGRLLLVLSCVGLLLTLAHPLRLRHCVLVLLLASFVWWVSFTSLGSLPSALRPLLSGLVGGAGCLLALGLDHFFQIREAPLHPRLSSAAEEKVPVIRPRRRSSCVSLGETAASYYGSCKIFRRPSLPCISREQMILWDWDLKQWYKPHYQNSGGGNGVDLSVLNEARNMVSDLLTDPSLPPQVISSLRSISSLMGAFSGSCRPKINPLTPFPGFYPCSEIEDPAEKGDRKLNKGLNRNSLPTPQLRRSSGTSGLLPVEQSSRWDRNNGKRPHQEFGISSQGCYLNGPFNSNLLTIPKQRSSSVSLTHHVGLRRAGVLSSLSPVNSSNHGPVSTGSLTNRSPIEFPDTADFLNKPSVILQRSLGNAPNTPDFYQQLRNSDSNLCNSCGHQMLKYVSTSESDGTDCCSGKSGEEENIFSKESFKLMETQQEEETEKKDSRKLFQEGDKWLTEEAQSEQQTNIEQEVSLDLILVEEYDSLIEKMSNWNFPIFELVEKMGEKSGRILSQVMYTLFQDTGLLEIFKIPTQQFMNYFRALENGYRDIPYHNRIHATDVLHAVWYLTTRPVPGLQQIHNGCGTGNETDSDGRINHGRIAYISSKSCSNPDESYGCLSSNIPALELMALYVAAAMHDYDHPGRTNAFLVATNAPQAVLYNDRSVLENHHAASAWNLYLSRPEYNFLLHLDHVEFKRFRFLVIEAILATDLKKHFDFLAEFNAKANDVNSNGIEWSNENDRLLVCQVCIKLADINGPAKVRDLHLKWTEGIVNEFYEQGDEEANLGLPISPFMDRSSPQLAKLQESFITHIVGPLCNSYDAAGLLPGQWLEAEEDNDTESGDDEDGEELDTEDEEMENNLNPKPPRRKSRRRIFCQLMHHLTENHKIWKEIVEEEEKCKADGNKLQVENSSLPQADEIQVIEEADEEE

Target ORF information:

RefSeq Version	NM_000922.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphodiesterase 3B (PDE3B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000922.3

ORF Insert Sequence:

ATGAGGAGGG ACGAGCGAGA CGCCAAAGCC ATGCGGTCCC TGCAGCCGCC GGATGGGGCC 
GGCTCGCCCC CCGAGAGTCT GAGGAACGGC TACGTGAAGA GCTGCGTGAG CCCCTTGCGG 
CAGGACCCTC CGCGCGGCTT CTTCTTCCAC CTCTGCCGCT TCTGCAACGT GGAGCTGCGG 
CCGCCGCCGG CCTCTCCCCA GCAGCCGCGG CGCTGCTCCC CCTTCTGCCG GGCGCGCCTC 
TCGCTGGGCG CCCTGGCTGC CTTTGTCCTC GCCCTGCTGC TGGGCGCGGA ACCCGAGAGC 
TGGGCTGCCG GGGCCGCCTG GCTGCGGACG CTGCTGAGCG TGTGTTCGCA CAGCTTGAGC 
CCCCTCTTCA GCATCGCCTG TGCCTTCTTC TTCCTCACCT GCTTCCTCAC CCGGACCAAG 
CGGGGACCCG GCCCGGGCCG GAGCTGCGGC TCCTGGTGGC TGCTGGCGCT GCCCGCCTGC 
TGTTACCTGG GGGACTTCTT GGTGTGGCAG TGGTGGTCTT GGCCTTGGGG GGATGGCGAC 
GCAGGGTCCG CGGCCCCGCA CACGCCCCCG GAGGCGGCAG CGGGCAGGTT GCTGCTGGTG 
CTGAGCTGCG TAGGGCTGCT GCTGACGCTC GCGCACCCGC TGCGGCTCCG GCACTGCGTT 
CTGGTGCTGC TCCTGGCCAG CTTCGTCTGG TGGGTCTCCT TCACCAGCCT CGGGTCGCTG 
CCCTCCGCCC TCAGGCCGCT GCTCTCCGGC CTGGTGGGGG GCGCTGGCTG CCTGCTGGCC 
CTGGGGTTGG ATCACTTCTT TCAAATCAGG GAAGCGCCTC TTCATCCTCG ACTGTCCAGT 
GCCGCCGAAG AAAAAGTGCC TGTGATCCGA CCCCGGAGGA GGTCCAGCTG CGTGTCGTTA 
GGAGAAACTG CAGCCAGTTA CTATGGCAGT TGCAAAATAT TCAGGAGACC GTCGTTGCCT 
TGTATTTCCA GAGAACAGAT GATTCTTTGG GATTGGGACT TAAAACAATG GTATAAGCCT 
CATTATCAAA ATTCTGGAGG TGGAAATGGA GTTGATCTTT CAGTGCTAAA TGAGGCTCGC 
AATATGGTGT CAGATCTTCT GACTGATCCA AGCCTTCCAC CACAAGTCAT TTCCTCTCTA 
CGGAGTATTA GTAGCTTAAT GGGTGCTTTC TCAGGTTCCT GTAGGCCAAA GATTAATCCT 
CTCACACCAT TTCCTGGATT TTACCCCTGT TCTGAAATAG AGGACCCAGC TGAGAAAGGG 
GATAGAAAAC TTAACAAGGG ACTAAATAGG AATAGTTTGC CAACTCCACA GCTGAGGAGA 
AGCTCAGGAA CTTCAGGATT GCTACCTGTT GAACAGTCTT CAAGGTGGGA TCGTAATAAT 
GGCAAAAGAC CTCACCAAGA ATTTGGCATT TCAAGTCAAG GATGCTATCT AAATGGGCCT 
TTTAATTCAA ATCTACTGAC TATCCCGAAG CAAAGGTCAT CTTCTGTATC ACTGACTCAC 
CATGTAGGTC TCAGAAGAGC TGGTGTTTTG TCCAGTCTGA GTCCTGTGAA TTCTTCCAAC 
CATGGACCAG TGTCTACTGG CTCTCTAACT AATCGATCAC CCATAGAATT TCCTGATACT 
GCTGATTTTC TTAATAAGCC AAGCGTTATC TTGCAGAGAT CTCTGGGCAA TGCACCTAAT 
ACTCCAGATT TTTATCAGCA ACTTAGAAAT TCTGATAGCA ATCTGTGTAA CAGCTGTGGA 
CATCAAATGC TGAAATATGT TTCAACATCT GAATCAGATG GTACAGATTG CTGCAGTGGA 
AAATCAGGTG AAGAAGAAAA CATTTTCTCG AAAGAATCAT TCAAACTTAT GGAAACTCAA 
CAAGAAGAGG AAACAGAGAA GAAAGACAGC AGAAAATTAT TTCAGGAAGG TGATAAGTGG 
CTAACAGAAG AGGCACAGAG TGAACAGCAA ACAAATATTG AACAGGAAGT ATCACTGGAC 
CTGATTTTAG TAGAAGAGTA TGACTCATTA ATAGAAAAGA TGAGCAACTG GAATTTTCCA 
ATTTTTGAAC TTGTAGAAAA GATGGGAGAG AAATCAGGAA GGATTCTCAG TCAGGTTATG 
TATACCTTAT TTCAAGACAC TGGTTTATTG GAAATATTTA AAATTCCCAC TCAACAATTT 
ATGAACTATT TTCGTGCATT AGAAAATGGC TATCGAGACA TTCCTTATCA CAATCGTATA 
CATGCCACAG ATGTGCTACA TGCAGTTTGG TATCTGACAA CACGGCCAGT TCCTGGCTTA 
CAGCAGATCC ACAATGGTTG TGGAACAGGA AATGAAACAG ATTCTGATGG TAGAATTAAC 
CATGGGCGAA TTGCTTATAT TTCTTCGAAG AGCTGCTCTA ATCCTGATGA GAGTTATGGC 
TGCCTGTCTT CAAACATTCC TGCATTAGAA TTGATGGCTC TATACGTGGC AGCTGCCATG 
CATGATTATG ATCACCCAGG GAGGACAAAT GCATTTCTAG TGGCTACAAA TGCCCCTCAG 
GCAGTTTTAT ACAATGACAG ATCTGTTCTG GAAAATCATC ATGCTGCGTC AGCTTGGAAT 
CTATATCTTT CTCGCCCAGA ATACAACTTC CTTCTTCATC TTGATCATGT GGAATTCAAG 
CGCTTTCGTT TTTTAGTCAT TGAAGCAATC CTTGCTACGG ATCTTAAAAA GCATTTTGAT 
TTTCTCGCAG AATTCAATGC CAAGGCAAAT GATGTAAATA GTAATGGCAT AGAATGGAGT 
AATGAAAATG ATCGCCTCTT GGTATGCCAG GTGTGCATCA AACTGGCAGA TATAAATGGC 
CCAGCAAAAG TTCGAGACTT GCATTTGAAA TGGACAGAAG GCATTGTCAA TGAATTTTAT 
GAGCAGGGAG ATGAAGAAGC AAATCTTGGT CTGCCCATCA GTCCATTCAT GGATCGTTCT 
TCTCCTCAAC TAGCAAAACT CCAAGAATCT TTTATCACCC ACATAGTGGG TCCCCTGTGT 
AACTCCTATG ATGCTGCTGG TTTGCTACCA GGTCAGTGGT TAGAAGCAGA AGAGGATAAT 
GATACTGAAA GTGGTGATGA TGAAGACGGT GAAGAATTAG ATACAGAAGA TGAAGAAATG 
GAAAACAATC TAAATCCAAA ACCACCAAGA AGGAAAAGCA GACGGCGAAT ATTTTGTCAG 
CTAATGCACC ACCTCACTGA AAACCACAAG ATATGGAAGG AAATCGTAGA GGAAGAAGAA 
AAATGTAAAG CTGATGGGAA TAAACTGCAG GTGGAGAATT CCTCCTTACC TCAAGCAGAT 
GAGATTCAGG TAATTGAAGA GGCAGATGAA GAGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE3B ( NM_000922.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000913.2 Homo sapiens phosphodiesterase 3B (PDE3B), transcript variant 2, mRNA.

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CloneID	OHu12511
Clone ID Related Accession (Same CDS sequence)	NM_000922.3 , NM_000922.4
Accession Version	NM_000922.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase B isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC150307.1, BC136565.1 and AC090835.7. On Nov 22, 2018 this sequence version replaced NM_000922.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.52394.1, SRR1660803.66885.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000282096.9/ ENSP00000282096.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGGAGGG ACGAGCGAGA CGCCAAAGCC ATGCGGTCCC TGCAGCCGCC GGATGGGGCC 
GGCTCGCCCC CCGAGAGTCT GAGGAACGGC TACGTGAAGA GCTGCGTGAG CCCCTTGCGG 
CAGGACCCTC CGCGCGGCTT CTTCTTCCAC CTCTGCCGCT TCTGCAACGT GGAGCTGCGG 
CCGCCGCCGG CCTCTCCCCA GCAGCCGCGG CGCTGCTCCC CCTTCTGCCG GGCGCGCCTC 
TCGCTGGGCG CCCTGGCTGC CTTTGTCCTC GCCCTGCTGC TGGGCGCGGA ACCCGAGAGC 
TGGGCTGCCG GGGCCGCCTG GCTGCGGACG CTGCTGAGCG TGTGTTCGCA CAGCTTGAGC 
CCCCTCTTCA GCATCGCCTG TGCCTTCTTC TTCCTCACCT GCTTCCTCAC CCGGACCAAG 
CGGGGACCCG GCCCGGGCCG GAGCTGCGGC TCCTGGTGGC TGCTGGCGCT GCCCGCCTGC 
TGTTACCTGG GGGACTTCTT GGTGTGGCAG TGGTGGTCTT GGCCTTGGGG GGATGGCGAC 
GCAGGGTCCG CGGCCCCGCA CACGCCCCCG GAGGCGGCAG CGGGCAGGTT GCTGCTGGTG 
CTGAGCTGCG TAGGGCTGCT GCTGACGCTC GCGCACCCGC TGCGGCTCCG GCACTGCGTT 
CTGGTGCTGC TCCTGGCCAG CTTCGTCTGG TGGGTCTCCT TCACCAGCCT CGGGTCGCTG 
CCCTCCGCCC TCAGGCCGCT GCTCTCCGGC CTGGTGGGGG GCGCTGGCTG CCTGCTGGCC 
CTGGGGTTGG ATCACTTCTT TCAAATCAGG GAAGCGCCTC TTCATCCTCG ACTGTCCAGT 
GCCGCCGAAG AAAAAGTGCC TGTGATCCGA CCCCGGAGGA GGTCCAGCTG CGTGTCGTTA 
GGAGAAACTG CAGCCAGTTA CTATGGCAGT TGCAAAATAT TCAGGAGACC GTCGTTGCCT 
TGTATTTCCA GAGAACAGAT GATTCTTTGG GATTGGGACT TAAAACAATG GTATAAGCCT 
CATTATCAAA ATTCTGGAGG TGGAAATGGA GTTGATCTTT CAGTGCTAAA TGAGGCTCGC 
AATATGGTGT CAGATCTTCT GACTGATCCA AGCCTTCCAC CACAAGTCAT TTCCTCTCTA 
CGGAGTATTA GTAGCTTAAT GGGTGCTTTC TCAGGTTCCT GTAGGCCAAA GATTAATCCT 
CTCACACCAT TTCCTGGATT TTACCCCTGT TCTGAAATAG AGGACCCAGC TGAGAAAGGG 
GATAGAAAAC TTAACAAGGG ACTAAATAGG AATAGTTTGC CAACTCCACA GCTGAGGAGA 
AGCTCAGGAA CTTCAGGATT GCTACCTGTT GAACAGTCTT CAAGGTGGGA TCGTAATAAT 
GGCAAAAGAC CTCACCAAGA ATTTGGCATT TCAAGTCAAG GATGCTATCT AAATGGGCCT 
TTTAATTCAA ATCTACTGAC TATCCCGAAG CAAAGGTCAT CTTCTGTATC ACTGACTCAC 
CATGTAGGTC TCAGAAGAGC TGGTGTTTTG TCCAGTCTGA GTCCTGTGAA TTCTTCCAAC 
CATGGACCAG TGTCTACTGG CTCTCTAACT AATCGATCAC CCATAGAATT TCCTGATACT 
GCTGATTTTC TTAATAAGCC AAGCGTTATC TTGCAGAGAT CTCTGGGCAA TGCACCTAAT 
ACTCCAGATT TTTATCAGCA ACTTAGAAAT TCTGATAGCA ATCTGTGTAA CAGCTGTGGA 
CATCAAATGC TGAAATATGT TTCAACATCT GAATCAGATG GTACAGATTG CTGCAGTGGA 
AAATCAGGTG AAGAAGAAAA CATTTTCTCG AAAGAATCAT TCAAACTTAT GGAAACTCAA 
CAAGAAGAGG AAACAGAGAA GAAAGACAGC AGAAAATTAT TTCAGGAAGG TGATAAGTGG 
CTAACAGAAG AGGCACAGAG TGAACAGCAA ACAAATATTG AACAGGAAGT ATCACTGGAC 
CTGATTTTAG TAGAAGAGTA TGACTCATTA ATAGAAAAGA TGAGCAACTG GAATTTTCCA 
ATTTTTGAAC TTGTAGAAAA GATGGGAGAG AAATCAGGAA GGATTCTCAG TCAGGTTATG 
TATACCTTAT TTCAAGACAC TGGTTTATTG GAAATATTTA AAATTCCCAC TCAACAATTT 
ATGAACTATT TTCGTGCATT AGAAAATGGC TATCGAGACA TTCCTTATCA CAATCGTATA 
CATGCCACAG ATGTGCTACA TGCAGTTTGG TATCTGACAA CACGGCCAGT TCCTGGCTTA 
CAGCAGATCC ACAATGGTTG TGGAACAGGA AATGAAACAG ATTCTGATGG TAGAATTAAC 
CATGGGCGAA TTGCTTATAT TTCTTCGAAG AGCTGCTCTA ATCCTGATGA GAGTTATGGC 
TGCCTGTCTT CAAACATTCC TGCATTAGAA TTGATGGCTC TATACGTGGC AGCTGCCATG 
CATGATTATG ATCACCCAGG GAGGACAAAT GCATTTCTAG TGGCTACAAA TGCCCCTCAG 
GCAGTTTTAT ACAATGACAG ATCTGTTCTG GAAAATCATC ATGCTGCGTC AGCTTGGAAT 
CTATATCTTT CTCGCCCAGA ATACAACTTC CTTCTTCATC TTGATCATGT GGAATTCAAG 
CGCTTTCGTT TTTTAGTCAT TGAAGCAATC CTTGCTACGG ATCTTAAAAA GCATTTTGAT 
TTTCTCGCAG AATTCAATGC CAAGGCAAAT GATGTAAATA GTAATGGCAT AGAATGGAGT 
AATGAAAATG ATCGCCTCTT GGTATGCCAG GTGTGCATCA AACTGGCAGA TATAAATGGC 
CCAGCAAAAG TTCGAGACTT GCATTTGAAA TGGACAGAAG GCATTGTCAA TGAATTTTAT 
GAGCAGGGAG ATGAAGAAGC AAATCTTGGT CTGCCCATCA GTCCATTCAT GGATCGTTCT 
TCTCCTCAAC TAGCAAAACT CCAAGAATCT TTTATCACCC ACATAGTGGG TCCCCTGTGT 
AACTCCTATG ATGCTGCTGG TTTGCTACCA GGTCAGTGGT TAGAAGCAGA AGAGGATAAT 
GATACTGAAA GTGGTGATGA TGAAGACGGT GAAGAATTAG ATACAGAAGA TGAAGAAATG 
GAAAACAATC TAAATCCAAA ACCACCAAGA AGGAAAAGCA GACGGCGAAT ATTTTGTCAG 
CTAATGCACC ACCTCACTGA AAACCACAAG ATATGGAAGG AAATCGTAGA GGAAGAAGAA 
AAATGTAAAG CTGATGGGAA TAAACTGCAG GTGGAGAATT CCTCCTTACC TCAAGCAGAT 
GAGATTCAGG TAATTGAAGA GGCAGATGAA GAGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000913.2
CDS	273..3611
Translation	MRRDERDAKAMRSLQPPDGAGSPPESLRNGYVKSCVSPLRQDPPRGFFFHLCRFCNVELRPPPASPQQPRRCSPFCRARLSLGALAAFVLALLLGAEPESWAAGAAWLRTLLSVCSHSLSPLFSIACAFFFLTCFLTRTKRGPGPGRSCGSWWLLALPACCYLGDFLVWQWWSWPWGDGDAGSAAPHTPPEAAAGRLLLVLSCVGLLLTLAHPLRLRHCVLVLLLASFVWWVSFTSLGSLPSALRPLLSGLVGGAGCLLALGLDHFFQIREAPLHPRLSSAAEEKVPVIRPRRRSSCVSLGETAASYYGSCKIFRRPSLPCISREQMILWDWDLKQWYKPHYQNSGGGNGVDLSVLNEARNMVSDLLTDPSLPPQVISSLRSISSLMGAFSGSCRPKINPLTPFPGFYPCSEIEDPAEKGDRKLNKGLNRNSLPTPQLRRSSGTSGLLPVEQSSRWDRNNGKRPHQEFGISSQGCYLNGPFNSNLLTIPKQRSSSVSLTHHVGLRRAGVLSSLSPVNSSNHGPVSTGSLTNRSPIEFPDTADFLNKPSVILQRSLGNAPNTPDFYQQLRNSDSNLCNSCGHQMLKYVSTSESDGTDCCSGKSGEEENIFSKESFKLMETQQEEETEKKDSRKLFQEGDKWLTEEAQSEQQTNIEQEVSLDLILVEEYDSLIEKMSNWNFPIFELVEKMGEKSGRILSQVMYTLFQDTGLLEIFKIPTQQFMNYFRALENGYRDIPYHNRIHATDVLHAVWYLTTRPVPGLQQIHNGCGTGNETDSDGRINHGRIAYISSKSCSNPDESYGCLSSNIPALELMALYVAAAMHDYDHPGRTNAFLVATNAPQAVLYNDRSVLENHHAASAWNLYLSRPEYNFLLHLDHVEFKRFRFLVIEAILATDLKKHFDFLAEFNAKANDVNSNGIEWSNENDRLLVCQVCIKLADINGPAKVRDLHLKWTEGIVNEFYEQGDEEANLGLPISPFMDRSSPQLAKLQESFITHIVGPLCNSYDAAGLLPGQWLEAEEDNDTESGDDEDGEELDTEDEEMENNLNPKPPRRKSRRRIFCQLMHHLTENHKIWKEIVEEEEKCKADGNKLQVENSSLPQADEIQVIEEADEEE

Target ORF information:

RefSeq Version	NM_000922.4
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphodiesterase 3B (PDE3B), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000922.4

ORF Insert Sequence:

ATGAGGAGGG ACGAGCGAGA CGCCAAAGCC ATGCGGTCCC TGCAGCCGCC GGATGGGGCC 
GGCTCGCCCC CCGAGAGTCT GAGGAACGGC TACGTGAAGA GCTGCGTGAG CCCCTTGCGG 
CAGGACCCTC CGCGCGGCTT CTTCTTCCAC CTCTGCCGCT TCTGCAACGT GGAGCTGCGG 
CCGCCGCCGG CCTCTCCCCA GCAGCCGCGG CGCTGCTCCC CCTTCTGCCG GGCGCGCCTC 
TCGCTGGGCG CCCTGGCTGC CTTTGTCCTC GCCCTGCTGC TGGGCGCGGA ACCCGAGAGC 
TGGGCTGCCG GGGCCGCCTG GCTGCGGACG CTGCTGAGCG TGTGTTCGCA CAGCTTGAGC 
CCCCTCTTCA GCATCGCCTG TGCCTTCTTC TTCCTCACCT GCTTCCTCAC CCGGACCAAG 
CGGGGACCCG GCCCGGGCCG GAGCTGCGGC TCCTGGTGGC TGCTGGCGCT GCCCGCCTGC 
TGTTACCTGG GGGACTTCTT GGTGTGGCAG TGGTGGTCTT GGCCTTGGGG GGATGGCGAC 
GCAGGGTCCG CGGCCCCGCA CACGCCCCCG GAGGCGGCAG CGGGCAGGTT GCTGCTGGTG 
CTGAGCTGCG TAGGGCTGCT GCTGACGCTC GCGCACCCGC TGCGGCTCCG GCACTGCGTT 
CTGGTGCTGC TCCTGGCCAG CTTCGTCTGG TGGGTCTCCT TCACCAGCCT CGGGTCGCTG 
CCCTCCGCCC TCAGGCCGCT GCTCTCCGGC CTGGTGGGGG GCGCTGGCTG CCTGCTGGCC 
CTGGGGTTGG ATCACTTCTT TCAAATCAGG GAAGCGCCTC TTCATCCTCG ACTGTCCAGT 
GCCGCCGAAG AAAAAGTGCC TGTGATCCGA CCCCGGAGGA GGTCCAGCTG CGTGTCGTTA 
GGAGAAACTG CAGCCAGTTA CTATGGCAGT TGCAAAATAT TCAGGAGACC GTCGTTGCCT 
TGTATTTCCA GAGAACAGAT GATTCTTTGG GATTGGGACT TAAAACAATG GTATAAGCCT 
CATTATCAAA ATTCTGGAGG TGGAAATGGA GTTGATCTTT CAGTGCTAAA TGAGGCTCGC 
AATATGGTGT CAGATCTTCT GACTGATCCA AGCCTTCCAC CACAAGTCAT TTCCTCTCTA 
CGGAGTATTA GTAGCTTAAT GGGTGCTTTC TCAGGTTCCT GTAGGCCAAA GATTAATCCT 
CTCACACCAT TTCCTGGATT TTACCCCTGT TCTGAAATAG AGGACCCAGC TGAGAAAGGG 
GATAGAAAAC TTAACAAGGG ACTAAATAGG AATAGTTTGC CAACTCCACA GCTGAGGAGA 
AGCTCAGGAA CTTCAGGATT GCTACCTGTT GAACAGTCTT CAAGGTGGGA TCGTAATAAT 
GGCAAAAGAC CTCACCAAGA ATTTGGCATT TCAAGTCAAG GATGCTATCT AAATGGGCCT 
TTTAATTCAA ATCTACTGAC TATCCCGAAG CAAAGGTCAT CTTCTGTATC ACTGACTCAC 
CATGTAGGTC TCAGAAGAGC TGGTGTTTTG TCCAGTCTGA GTCCTGTGAA TTCTTCCAAC 
CATGGACCAG TGTCTACTGG CTCTCTAACT AATCGATCAC CCATAGAATT TCCTGATACT 
GCTGATTTTC TTAATAAGCC AAGCGTTATC TTGCAGAGAT CTCTGGGCAA TGCACCTAAT 
ACTCCAGATT TTTATCAGCA ACTTAGAAAT TCTGATAGCA ATCTGTGTAA CAGCTGTGGA 
CATCAAATGC TGAAATATGT TTCAACATCT GAATCAGATG GTACAGATTG CTGCAGTGGA 
AAATCAGGTG AAGAAGAAAA CATTTTCTCG AAAGAATCAT TCAAACTTAT GGAAACTCAA 
CAAGAAGAGG AAACAGAGAA GAAAGACAGC AGAAAATTAT TTCAGGAAGG TGATAAGTGG 
CTAACAGAAG AGGCACAGAG TGAACAGCAA ACAAATATTG AACAGGAAGT ATCACTGGAC 
CTGATTTTAG TAGAAGAGTA TGACTCATTA ATAGAAAAGA TGAGCAACTG GAATTTTCCA 
ATTTTTGAAC TTGTAGAAAA GATGGGAGAG AAATCAGGAA GGATTCTCAG TCAGGTTATG 
TATACCTTAT TTCAAGACAC TGGTTTATTG GAAATATTTA AAATTCCCAC TCAACAATTT 
ATGAACTATT TTCGTGCATT AGAAAATGGC TATCGAGACA TTCCTTATCA CAATCGTATA 
CATGCCACAG ATGTGCTACA TGCAGTTTGG TATCTGACAA CACGGCCAGT TCCTGGCTTA 
CAGCAGATCC ACAATGGTTG TGGAACAGGA AATGAAACAG ATTCTGATGG TAGAATTAAC 
CATGGGCGAA TTGCTTATAT TTCTTCGAAG AGCTGCTCTA ATCCTGATGA GAGTTATGGC 
TGCCTGTCTT CAAACATTCC TGCATTAGAA TTGATGGCTC TATACGTGGC AGCTGCCATG 
CATGATTATG ATCACCCAGG GAGGACAAAT GCATTTCTAG TGGCTACAAA TGCCCCTCAG 
GCAGTTTTAT ACAATGACAG ATCTGTTCTG GAAAATCATC ATGCTGCGTC AGCTTGGAAT 
CTATATCTTT CTCGCCCAGA ATACAACTTC CTTCTTCATC TTGATCATGT GGAATTCAAG 
CGCTTTCGTT TTTTAGTCAT TGAAGCAATC CTTGCTACGG ATCTTAAAAA GCATTTTGAT 
TTTCTCGCAG AATTCAATGC CAAGGCAAAT GATGTAAATA GTAATGGCAT AGAATGGAGT 
AATGAAAATG ATCGCCTCTT GGTATGCCAG GTGTGCATCA AACTGGCAGA TATAAATGGC 
CCAGCAAAAG TTCGAGACTT GCATTTGAAA TGGACAGAAG GCATTGTCAA TGAATTTTAT 
GAGCAGGGAG ATGAAGAAGC AAATCTTGGT CTGCCCATCA GTCCATTCAT GGATCGTTCT 
TCTCCTCAAC TAGCAAAACT CCAAGAATCT TTTATCACCC ACATAGTGGG TCCCCTGTGT 
AACTCCTATG ATGCTGCTGG TTTGCTACCA GGTCAGTGGT TAGAAGCAGA AGAGGATAAT 
GATACTGAAA GTGGTGATGA TGAAGACGGT GAAGAATTAG ATACAGAAGA TGAAGAAATG 
GAAAACAATC TAAATCCAAA ACCACCAAGA AGGAAAAGCA GACGGCGAAT ATTTTGTCAG 
CTAATGCACC ACCTCACTGA AAACCACAAG ATATGGAAGG AAATCGTAGA GGAAGAAGAA 
AAATGTAAAG CTGATGGGAA TAAACTGCAG GTGGAGAATT CCTCCTTACC TCAAGCAGAT 
GAGATTCAGG TAATTGAAGA GGCAGATGAA GAGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE3B ( NM_001363569.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350498.1 Homo sapiens phosphodiesterase 3B (PDE3B), transcript variant 3, mRNA.

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CloneID	OHu106784
Clone ID Related Accession (Same CDS sequence)	NM_001363569.1
Accession Version	NM_001363569.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase B isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC087207.11, AC018795.10 and AC090835.7. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC144248.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2150385, SAMEA2154125 [ECO:0000348] ##Evidence-Data-END##

ATGAGGAGGG ACGAGCGAGA CGCCAAAGCC ATGCGGTCCC TGCAGCCGCC GGATGGGGCC 
GGCTCGCCCC CCGAGAGTCT GAGGAACGGC TACGTGAAGA GCTGCGTGAG CCCCTTGCGG 
CAGGACCCTC CGCGCGGCTT CTTCTTCCAC CTCTGCCGCT TCTGCAACGT GGAGCTGCGG 
CCGCCGCCGG CCTCTCCCCA GCAGCCGCGG CGCTGCTCCC CCTTCTGCCG GGCGCGCCTC 
TCGCTGGGCG CCCTGGCTGC CTTTGTCCTC GCCCTGCTGC TGGGCGCGGA ACCCGAGAGC 
TGGGCTGCCG GGGCCGCCTG GCTGCGGACG CTGCTGAGCG TGTGTTCGCA CAGCTTGAGC 
CCCCTCTTCA GCATCGCCTG TGCCTTCTTC TTCCTCACCT GCTTCCTCAC CCGGACCAAG 
CGGGGACCCG GCCCGGGCCG GAGCTGCGGC TCCTGGTGGC TGCTGGCGCT GCCCGCCTGC 
TGTTACCTGG GGGACTTCTT GGTGTGGCAG TGGTGGTCTT GGCCTTGGGG GGATGGCGAC 
GCAGGGTCCG CGGCCCCGCA CACGCCCCCG GAGGCGGCAG CGGGCAGGTT GCTGCTGGTG 
CTGAGCTGCG TAGGGCTGCT GCTGACGCTC GCGCACCCGC TGCGGCTCCG GCACTGCGTT 
CTGGTGCTGC TCCTGGCCAG CTTCGTCTGG TGGGTCTCCT TCACCAGCCT CGGGTCGCTG 
CCCTCCGCCC TCAGGCCGCT GCTCTCCGGC CTGGTGGGGG GCGCTGGCTG CCTGCTGGCC 
CTGGGGTTGG ATCACTTCTT TCAAATCAGG GAAGCGCCTC TTCATCCTCG ACTGTCCAGT 
GCCGCCGAAG AAAAAGTGCC TGTGATCCGA CCCCGGAGGA GGTCCAGCTG CGTGTCGTTA 
GGAGAAACTG CAGCCAGTTA CTATGGCAGT TGCAAAATAT TCAGGAGACC GTCGTTGCCT 
TGTATTTCCA GAGAACAGAT GATTCTTTGG GATTGGGACT TAAAACAATG GTATAAGCCT 
CATTATCAAA ATTCTGGAGG TGGAAATGGA GTTGATCTTT CAGTGCTAAA TGAGGCTCGC 
AATATGGTGT CAGATCTTCT GACTGATCCA AGCCTTCCAC CACAAGGACT AAATAGGAAT 
AGTTTGCCAA CTCCACAGCT GAGGAGAAGC TCAGGAACTT CAGGATTGCT ACCTGTTGAA 
CAGTCTTCAA GGTGGGATCG TAATAATGGC AAAAGACCTC ACCAAGAATT TGGCATTTCA 
AGTCAAGGAT GCTATCTAAA TGGGCCTTTT AATTCAAATC TACTGACTAT CCCGAAGCAA 
AGGTCATCTT CTGTATCACT GACTCACCAT GTAGGTCTCA GAAGAGCTGG TGTTTTGTCC 
AGTCTGAGTC CTGTGAATTC TTCCAACCAT GGACCAGTGT CTACTGGCTC TCTAACTAAT 
CGATCACCCA TAGAATTTCC TGATACTGCT GATTTTCTTA ATAAGCCAAG CGTTATCTTG 
CAGAGATCTC TGGGCAATGC ACCTAATACT CCAGATTTTT ATCAGCAACT TAGAAATTCT 
GATAGCAATC TGTGTAACAG CTGTGGACAT CAAATGCTGA AATATGTTTC AACATCTGAA 
TCAGATGGTA CAGATTGCTG CAGTGGAAAA TCAGGTGAAG AAGAAAACAT TTTCTCGAAA 
GAATCATTCA AACTTATGGA AACTCAACAA GAAGAGGAAA CAGAGAAGAA AGACAGCAGA 
AAATTATTTC AGGAAGGTGA TAAGTGGCTA ACAGAAGAGG CACAGAGTGA ACAGCAAACA 
AATATTGAAC AGGAAGTATC ACTGGACCTG ATTTTAGTAG AAGAGTATGA CTCATTAATA 
GAAAAGATGA GCAACTGGAA TTTTCCAATT TTTGAACTTG TAGAAAAGAT GGGAGAGAAA 
TCAGGAAGGA TTCTCAGTCA GGTTATGTAT ACCTTATTTC AAGACACTGG TTTATTGGAA 
ATATTTAAAA TTCCCACTCA ACAATTTATG AACTATTTTC GTGCATTAGA AAATGGCTAT 
CGAGACATTC CTTATCACAA TCGTATACAT GCCACAGATG TGCTACATGC AGTTTGGTAT 
CTGACAACAC GGCCAGTTCC TGGCTTACAG CAGATCCACA ATGGTTGTGG AACAGGAAAT 
GAAACAGATT CTGATGGTAG AATTAACCAT GGGCGAATTG CTTATATTTC TTCGAAGAGC 
TGCTCTAATC CTGATGAGAG TTATGGCTGC CTGTCTTCAA ACATTCCTGC ATTAGAATTG 
ATGGCTCTAT ACGTGGCAGC TGCCATGCAT GATTATGATC ACCCAGGGAG GACAAATGCA 
TTTCTAGTGG CTACAAATGC CCCTCAGGCA GTTTTATACA ATGACAGATC TGTTCTGGAA 
AATCATCATG CTGCGTCAGC TTGGAATCTA TATCTTTCTC GCCCAGAATA CAACTTCCTT 
CTTCATCTTG ATCATGTGGA ATTCAAGCGC TTTCGTTTTT TAGTCATTGA AGCAATCCTT 
GCTACGGATC TTAAAAAGCA TTTTGATTTT CTCGCAGAAT TCAATGCCAA GGCAAATGAT 
GTAAATAGTA ATGGCATAGA ATGGAGTAAT GAAAATGATC GCCTCTTGGT ATGCCAGGTG 
TGCATCAAAC TGGCAGATAT AAATGGCCCA GCAAAAGTTC GAGACTTGCA TTTGAAATGG 
ACAGAAGGCA TTGTCAATGA ATTTTATGAG CAGGGAGATG AAGAAGCAAA TCTTGGTCTG 
CCCATCAGTC CATTCATGGA TCGTTCTTCT CCTCAACTAG CAAAACTCCA AGAATCTTTT 
ATCACCCACA TAGTGGGTCC CCTGTGTAAC TCCTATGATG CTGCTGGTTT GCTACCAGGT 
CAGTGGTTAG AAGCAGAAGA GGATAATGAT ACTGAAAGTG GTGATGATGA AGACGGTGAA 
GAATTAGATA CAGAAGATGA AGAAATGGAA AACAATCTAA ATCCAAAACC ACCAAGAAGG 
AAAAGCAGAC GGCGAATATT TTGTCAGCTA ATGCACCACC TCACTGAAAA CCACAAGATA 
TGGAAGGAAA TCGTAGAGGA AGAAGAAAAA TGTAAAGCTG ATGGGAATAA ACTGCAGGTG 
GAGAATTCCT CCTTACCTCA AGCAGATGAG ATTCAGGTAA TTGAAGAGGC AGATGAAGAG 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350498.1
CDS	354..3539
Translation	MRRDERDAKAMRSLQPPDGAGSPPESLRNGYVKSCVSPLRQDPPRGFFFHLCRFCNVELRPPPASPQQPRRCSPFCRARLSLGALAAFVLALLLGAEPESWAAGAAWLRTLLSVCSHSLSPLFSIACAFFFLTCFLTRTKRGPGPGRSCGSWWLLALPACCYLGDFLVWQWWSWPWGDGDAGSAAPHTPPEAAAGRLLLVLSCVGLLLTLAHPLRLRHCVLVLLLASFVWWVSFTSLGSLPSALRPLLSGLVGGAGCLLALGLDHFFQIREAPLHPRLSSAAEEKVPVIRPRRRSSCVSLGETAASYYGSCKIFRRPSLPCISREQMILWDWDLKQWYKPHYQNSGGGNGVDLSVLNEARNMVSDLLTDPSLPPQGLNRNSLPTPQLRRSSGTSGLLPVEQSSRWDRNNGKRPHQEFGISSQGCYLNGPFNSNLLTIPKQRSSSVSLTHHVGLRRAGVLSSLSPVNSSNHGPVSTGSLTNRSPIEFPDTADFLNKPSVILQRSLGNAPNTPDFYQQLRNSDSNLCNSCGHQMLKYVSTSESDGTDCCSGKSGEEENIFSKESFKLMETQQEEETEKKDSRKLFQEGDKWLTEEAQSEQQTNIEQEVSLDLILVEEYDSLIEKMSNWNFPIFELVEKMGEKSGRILSQVMYTLFQDTGLLEIFKIPTQQFMNYFRALENGYRDIPYHNRIHATDVLHAVWYLTTRPVPGLQQIHNGCGTGNETDSDGRINHGRIAYISSKSCSNPDESYGCLSSNIPALELMALYVAAAMHDYDHPGRTNAFLVATNAPQAVLYNDRSVLENHHAASAWNLYLSRPEYNFLLHLDHVEFKRFRFLVIEAILATDLKKHFDFLAEFNAKANDVNSNGIEWSNENDRLLVCQVCIKLADINGPAKVRDLHLKWTEGIVNEFYEQGDEEANLGLPISPFMDRSSPQLAKLQESFITHIVGPLCNSYDAAGLLPGQWLEAEEDNDTESGDDEDGEELDTEDEEMENNLNPKPPRRKSRRRIFCQLMHHLTENHKIWKEIVEEEEKCKADGNKLQVENSSLPQADEIQVIEEADEEE

Target ORF information:

RefSeq Version	NM_001363569.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphodiesterase 3B (PDE3B), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363569.1

ORF Insert Sequence:

ATGAGGAGGG ACGAGCGAGA CGCCAAAGCC ATGCGGTCCC TGCAGCCGCC GGATGGGGCC 
GGCTCGCCCC CCGAGAGTCT GAGGAACGGC TACGTGAAGA GCTGCGTGAG CCCCTTGCGG 
CAGGACCCTC CGCGCGGCTT CTTCTTCCAC CTCTGCCGCT TCTGCAACGT GGAGCTGCGG 
CCGCCGCCGG CCTCTCCCCA GCAGCCGCGG CGCTGCTCCC CCTTCTGCCG GGCGCGCCTC 
TCGCTGGGCG CCCTGGCTGC CTTTGTCCTC GCCCTGCTGC TGGGCGCGGA ACCCGAGAGC 
TGGGCTGCCG GGGCCGCCTG GCTGCGGACG CTGCTGAGCG TGTGTTCGCA CAGCTTGAGC 
CCCCTCTTCA GCATCGCCTG TGCCTTCTTC TTCCTCACCT GCTTCCTCAC CCGGACCAAG 
CGGGGACCCG GCCCGGGCCG GAGCTGCGGC TCCTGGTGGC TGCTGGCGCT GCCCGCCTGC 
TGTTACCTGG GGGACTTCTT GGTGTGGCAG TGGTGGTCTT GGCCTTGGGG GGATGGCGAC 
GCAGGGTCCG CGGCCCCGCA CACGCCCCCG GAGGCGGCAG CGGGCAGGTT GCTGCTGGTG 
CTGAGCTGCG TAGGGCTGCT GCTGACGCTC GCGCACCCGC TGCGGCTCCG GCACTGCGTT 
CTGGTGCTGC TCCTGGCCAG CTTCGTCTGG TGGGTCTCCT TCACCAGCCT CGGGTCGCTG 
CCCTCCGCCC TCAGGCCGCT GCTCTCCGGC CTGGTGGGGG GCGCTGGCTG CCTGCTGGCC 
CTGGGGTTGG ATCACTTCTT TCAAATCAGG GAAGCGCCTC TTCATCCTCG ACTGTCCAGT 
GCCGCCGAAG AAAAAGTGCC TGTGATCCGA CCCCGGAGGA GGTCCAGCTG CGTGTCGTTA 
GGAGAAACTG CAGCCAGTTA CTATGGCAGT TGCAAAATAT TCAGGAGACC GTCGTTGCCT 
TGTATTTCCA GAGAACAGAT GATTCTTTGG GATTGGGACT TAAAACAATG GTATAAGCCT 
CATTATCAAA ATTCTGGAGG TGGAAATGGA GTTGATCTTT CAGTGCTAAA TGAGGCTCGC 
AATATGGTGT CAGATCTTCT GACTGATCCA AGCCTTCCAC CACAAGGACT AAATAGGAAT 
AGTTTGCCAA CTCCACAGCT GAGGAGAAGC TCAGGAACTT CAGGATTGCT ACCTGTTGAA 
CAGTCTTCAA GGTGGGATCG TAATAATGGC AAAAGACCTC ACCAAGAATT TGGCATTTCA 
AGTCAAGGAT GCTATCTAAA TGGGCCTTTT AATTCAAATC TACTGACTAT CCCGAAGCAA 
AGGTCATCTT CTGTATCACT GACTCACCAT GTAGGTCTCA GAAGAGCTGG TGTTTTGTCC 
AGTCTGAGTC CTGTGAATTC TTCCAACCAT GGACCAGTGT CTACTGGCTC TCTAACTAAT 
CGATCACCCA TAGAATTTCC TGATACTGCT GATTTTCTTA ATAAGCCAAG CGTTATCTTG 
CAGAGATCTC TGGGCAATGC ACCTAATACT CCAGATTTTT ATCAGCAACT TAGAAATTCT 
GATAGCAATC TGTGTAACAG CTGTGGACAT CAAATGCTGA AATATGTTTC AACATCTGAA 
TCAGATGGTA CAGATTGCTG CAGTGGAAAA TCAGGTGAAG AAGAAAACAT TTTCTCGAAA 
GAATCATTCA AACTTATGGA AACTCAACAA GAAGAGGAAA CAGAGAAGAA AGACAGCAGA 
AAATTATTTC AGGAAGGTGA TAAGTGGCTA ACAGAAGAGG CACAGAGTGA ACAGCAAACA 
AATATTGAAC AGGAAGTATC ACTGGACCTG ATTTTAGTAG AAGAGTATGA CTCATTAATA 
GAAAAGATGA GCAACTGGAA TTTTCCAATT TTTGAACTTG TAGAAAAGAT GGGAGAGAAA 
TCAGGAAGGA TTCTCAGTCA GGTTATGTAT ACCTTATTTC AAGACACTGG TTTATTGGAA 
ATATTTAAAA TTCCCACTCA ACAATTTATG AACTATTTTC GTGCATTAGA AAATGGCTAT 
CGAGACATTC CTTATCACAA TCGTATACAT GCCACAGATG TGCTACATGC AGTTTGGTAT 
CTGACAACAC GGCCAGTTCC TGGCTTACAG CAGATCCACA ATGGTTGTGG AACAGGAAAT 
GAAACAGATT CTGATGGTAG AATTAACCAT GGGCGAATTG CTTATATTTC TTCGAAGAGC 
TGCTCTAATC CTGATGAGAG TTATGGCTGC CTGTCTTCAA ACATTCCTGC ATTAGAATTG 
ATGGCTCTAT ACGTGGCAGC TGCCATGCAT GATTATGATC ACCCAGGGAG GACAAATGCA 
TTTCTAGTGG CTACAAATGC CCCTCAGGCA GTTTTATACA ATGACAGATC TGTTCTGGAA 
AATCATCATG CTGCGTCAGC TTGGAATCTA TATCTTTCTC GCCCAGAATA CAACTTCCTT 
CTTCATCTTG ATCATGTGGA ATTCAAGCGC TTTCGTTTTT TAGTCATTGA AGCAATCCTT 
GCTACGGATC TTAAAAAGCA TTTTGATTTT CTCGCAGAAT TCAATGCCAA GGCAAATGAT 
GTAAATAGTA ATGGCATAGA ATGGAGTAAT GAAAATGATC GCCTCTTGGT ATGCCAGGTG 
TGCATCAAAC TGGCAGATAT AAATGGCCCA GCAAAAGTTC GAGACTTGCA TTTGAAATGG 
ACAGAAGGCA TTGTCAATGA ATTTTATGAG CAGGGAGATG AAGAAGCAAA TCTTGGTCTG 
CCCATCAGTC CATTCATGGA TCGTTCTTCT CCTCAACTAG CAAAACTCCA AGAATCTTTT 
ATCACCCACA TAGTGGGTCC CCTGTGTAAC TCCTATGATG CTGCTGGTTT GCTACCAGGT 
CAGTGGTTAG AAGCAGAAGA GGATAATGAT ACTGAAAGTG GTGATGATGA AGACGGTGAA 
GAATTAGATA CAGAAGATGA AGAAATGGAA AACAATCTAA ATCCAAAACC ACCAAGAAGG 
AAAAGCAGAC GGCGAATATT TTGTCAGCTA ATGCACCACC TCACTGAAAA CCACAAGATA 
TGGAAGGAAA TCGTAGAGGA AGAAGAAAAA TGTAAAGCTG ATGGGAATAA ACTGCAGGTG 
GAGAATTCCT CCTTACCTCA AGCAGATGAG ATTCAGGTAA TTGAAGAGGC AGATGAAGAG 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE3B ( NM_001363570.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350499.1 Homo sapiens phosphodiesterase 3B (PDE3B), transcript variant 1, mRNA.

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CloneID	OHu107571
Clone ID Related Accession (Same CDS sequence)	NM_001363570.1
Accession Version	NM_001363570.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase B isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC087207.11, AC018795.10 and AC090835.7. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.205989.1, SRR1803615.44739.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142680, SAMEA2153980 [ECO:0000348] ##Evidence-Data-END##

ATGAGGAGGG ACGAGCGAGA CGCCAAAGCC ATGCGGTCCC TGCAGCCGCC GGATGGGGCC 
GGCTCGCCCC CCGAGAGTCT GAGGAACGGC TACGTGAAGA GCTGCGTGAG CCCCTTGCGG 
CAGGACCCTC CGCGCGGCTT CTTCTTCCAC CTCTGCCGCT TCTGCAACGT GGAGCTGCGG 
CCGCCGCCGG CCTCTCCCCA GCAGCCGCGG CGCTGCTCCC CCTTCTGCCG GGCGCGCCTC 
TCGCTGGGCG CCCTGGCTGC CTTTGTCCTC GCCCTGCTGC TGGGCGCGGA ACCCGAGAGC 
TGGGCTGCCG GGGCCGCCTG GCTGCGGACG CTGCTGAGCG TGTGTTCGCA CAGCTTGAGC 
CCCCTCTTCA GCATCGCCTG TGCCTTCTTC TTCCTCACCT GCTTCCTCAC CCGGACCAAG 
CGGGGACCCG GCCCGGGCCG GAGCTGCGGC TCCTGGTGGC TGCTGGCGCT GCCCGCCTGC 
TGTTACCTGG GGGACTTCTT GGTGTGGCAG TGGTGGTCTT GGCCTTGGGG GGATGGCGAC 
GCAGGGTCCG CGGCCCCGCA CACGCCCCCG GAGGCGGCAG CGGGCAGGTT GCTGCTGGTG 
CTGAGCTGCG TAGGGCTGCT GCTGACGCTC GCGCACCCGC TGCGGCTCCG GCACTGCGTT 
CTGGTGCTGC TCCTGGCCAG CTTCGTCTGG TGGGTCTCCT TCACCAGCCT CGGGTCGCTG 
CCCTCCGCCC TCAGGCCGCT GCTCTCCGGC CTGGTGGGGG GCGCTGGCTG CCTGCTGGCC 
CTGGGGTTGG ATCACTTCTT TCAAATCAGG GAAGCGCCTC TTCATCCTCG ACTGTCCAGT 
GCCGCCGAAG AAAAAGTGCC TGTGATCCGA CCCCGGAGGA GGTCCAGCTG CGTGTCGTTA 
GGAGAAACTG CAGCCAGTTA CTATGGCAGT TGCAAAATAT TCAGGAGACC GTCGTTGCCT 
TGTATTTCCA GAGAACAGAT GATTCTTTGG GATTGGGACT TAAAACAATG GTATAAGCCT 
CATTATCAAG CTGGAGTGCA GTGGCATGAT CTCGGCTCAC CGCAACCTCC AGCTCCCTGG 
TTCAAGCGAT TCTCCTGCCT CAGCCTCCGG AGTAGCTGGG ATTACAGGCA TGTGCCACCA 
CACCTGGCTA ATTTTGTATT TTTAGTAGAG ATGGGATTTC TCCATGTTGG TCAGGCTGGT 
CTTGAACTCC CGACCTCAGG TGATCTGCCC ACCTCGGCCT CCCAAAGTGC TGGGATTACA 
GGGAATTCTG GAGGTGGAAA TGGAGTTGAT CTTTCAGTGC TAAATGAGGC TCGCAATATG 
GTGTCAGATC TTCTGACTGA TCCAAGCCTT CCACCACAAG TCATTTCCTC TCTACGGAGT 
ATTAGTAGCT TAATGGGTGC TTTCTCAGGT TCCTGTAGGC CAAAGATTAA TCCTCTCACA 
CCATTTCCTG GATTTTACCC CTGTTCTGAA ATAGAGGACC CAGCTGAGAA AGGGGATAGA 
AAACTTAACA AGGGACTAAA TAGGAATAGT TTGCCAACTC CACAGCTGAG GAGAAGCTCA 
GGAACTTCAG GATTGCTACC TGTTGAACAG TCTTCAAGGT GGGATCGTAA TAATGGCAAA 
AGACCTCACC AAGAATTTGG CATTTCAAGT CAAGGATGCT ATCTAAATGG GCCTTTTAAT 
TCAAATCTAC TGACTATCCC GAAGCAAAGG TCATCTTCTG TATCACTGAC TCACCATGTA 
GGTCTCAGAA GAGCTGGTGT TTTGTCCAGT CTGAGTCCTG TGAATTCTTC CAACCATGGA 
CCAGTGTCTA CTGGCTCTCT AACTAATCGA TCACCCATAG AATTTCCTGA TACTGCTGAT 
TTTCTTAATA AGCCAAGCGT TATCTTGCAG AGATCTCTGG GCAATGCACC TAATACTCCA 
GATTTTTATC AGCAACTTAG AAATTCTGAT AGCAATCTGT GTAACAGCTG TGGACATCAA 
ATGCTGAAAT ATGTTTCAAC ATCTGAATCA GATGGTACAG ATTGCTGCAG TGGAAAATCA 
GGTGAAGAAG AAAACATTTT CTCGAAAGAA TCATTCAAAC TTATGGAAAC TCAACAAGAA 
GAGGAAACAG AGAAGAAAGA CAGCAGAAAA TTATTTCAGG AAGGTGATAA GTGGCTAACA 
GAAGAGGCAC AGAGTGAACA GCAAACAAAT ATTGAACAGG AAGTATCACT GGACCTGATT 
TTAGTAGAAG AGTATGACTC ATTAATAGAA AAGATGAGCA ACTGGAATTT TCCAATTTTT 
GAACTTGTAG AAAAGATGGG AGAGAAATCA GGAAGGATTC TCAGTCAGGT TATGTATACC 
TTATTTCAAG ACACTGGTTT ATTGGAAATA TTTAAAATTC CCACTCAACA ATTTATGAAC 
TATTTTCGTG CATTAGAAAA TGGCTATCGA GACATTCCTT ATCACAATCG TATACATGCC 
ACAGATGTGC TACATGCAGT TTGGTATCTG ACAACACGGC CAGTTCCTGG CTTACAGCAG 
ATCCACAATG GTTGTGGAAC AGGAAATGAA ACAGATTCTG ATGGTAGAAT TAACCATGGG 
CGAATTGCTT ATATTTCTTC GAAGAGCTGC TCTAATCCTG ATGAGAGTTA TGGCTGCCTG 
TCTTCAAACA TTCCTGCATT AGAATTGATG GCTCTATACG TGGCAGCTGC CATGCATGAT 
TATGATCACC CAGGGAGGAC AAATGCATTT CTAGTGGCTA CAAATGCCCC TCAGGCAGTT 
TTATACAATG ACAGATCTGT TCTGGAAAAT CATCATGCTG CGTCAGCTTG GAATCTATAT 
CTTTCTCGCC CAGAATACAA CTTCCTTCTT CATCTTGATC ATGTGGAATT CAAGCGCTTT 
CGTTTTTTAG TCATTGAAGC AATCCTTGCT ACGGATCTTA AAAAGCATTT TGATTTTCTC 
GCAGAATTCA ATGCCAAGGC AAATGATGTA AATAGTAATG GCATAGAATG GAGTAATGAA 
AATGATCGCC TCTTGGTATG CCAGGTGTGC ATCAAACTGG CAGATATAAA TGGCCCAGCA 
AAAGTTCGAG ACTTGCATTT GAAATGGACA GAAGGCATTG TCAATGAATT TTATGAGCAG 
GGAGATGAAG AAGCAAATCT TGGTCTGCCC ATCAGTCCAT TCATGGATCG TTCTTCTCCT 
CAACTAGCAA AACTCCAAGA ATCTTTTATC ACCCACATAG TGGGTCCCCT GTGTAACTCC 
TATGATGCTG CTGGTTTGCT ACCAGGTCAG TGGTTAGAAG CAGAAGAGGA TAATGATACT 
GAAAGTGGTG ATGATGAAGA CGGTGAAGAA TTAGATACAG AAGATGAAGA AATGGAAAAC 
AATCTAAATC CAAAACCACC AAGAAGGAAA AGCAGACGGC GAATATTTTG TCAGCTAATG 
CACCACCTCA CTGAAAACCA CAAGATATGG AAGGAAATCG TAGAGGAAGA AGAAAAATGT 
AAAGCTGATG GGAATAAACT GCAGGTGGAG AATTCCTCCT TACCTCAAGC AGATGAGATT 
CAGGTAATTG AAGAGGCAGA TGAAGAGGAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350499.1
CDS	354..3926
Translation	MRRDERDAKAMRSLQPPDGAGSPPESLRNGYVKSCVSPLRQDPPRGFFFHLCRFCNVELRPPPASPQQPRRCSPFCRARLSLGALAAFVLALLLGAEPESWAAGAAWLRTLLSVCSHSLSPLFSIACAFFFLTCFLTRTKRGPGPGRSCGSWWLLALPACCYLGDFLVWQWWSWPWGDGDAGSAAPHTPPEAAAGRLLLVLSCVGLLLTLAHPLRLRHCVLVLLLASFVWWVSFTSLGSLPSALRPLLSGLVGGAGCLLALGLDHFFQIREAPLHPRLSSAAEEKVPVIRPRRRSSCVSLGETAASYYGSCKIFRRPSLPCISREQMILWDWDLKQWYKPHYQAGVQWHDLGSPQPPAPWFKRFSCLSLRSSWDYRHVPPHLANFVFLVEMGFLHVGQAGLELPTSGDLPTSASQSAGITGNSGGGNGVDLSVLNEARNMVSDLLTDPSLPPQVISSLRSISSLMGAFSGSCRPKINPLTPFPGFYPCSEIEDPAEKGDRKLNKGLNRNSLPTPQLRRSSGTSGLLPVEQSSRWDRNNGKRPHQEFGISSQGCYLNGPFNSNLLTIPKQRSSSVSLTHHVGLRRAGVLSSLSPVNSSNHGPVSTGSLTNRSPIEFPDTADFLNKPSVILQRSLGNAPNTPDFYQQLRNSDSNLCNSCGHQMLKYVSTSESDGTDCCSGKSGEEENIFSKESFKLMETQQEEETEKKDSRKLFQEGDKWLTEEAQSEQQTNIEQEVSLDLILVEEYDSLIEKMSNWNFPIFELVEKMGEKSGRILSQVMYTLFQDTGLLEIFKIPTQQFMNYFRALENGYRDIPYHNRIHATDVLHAVWYLTTRPVPGLQQIHNGCGTGNETDSDGRINHGRIAYISSKSCSNPDESYGCLSSNIPALELMALYVAAAMHDYDHPGRTNAFLVATNAPQAVLYNDRSVLENHHAASAWNLYLSRPEYNFLLHLDHVEFKRFRFLVIEAILATDLKKHFDFLAEFNAKANDVNSNGIEWSNENDRLLVCQVCIKLADINGPAKVRDLHLKWTEGIVNEFYEQGDEEANLGLPISPFMDRSSPQLAKLQESFITHIVGPLCNSYDAAGLLPGQWLEAEEDNDTESGDDEDGEELDTEDEEMENNLNPKPPRRKSRRRIFCQLMHHLTENHKIWKEIVEEEEKCKADGNKLQVENSSLPQADEIQVIEEADEEE

Target ORF information:

RefSeq Version	NM_001363570.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphodiesterase 3B (PDE3B), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363570.1

ORF Insert Sequence:

ATGAGGAGGG ACGAGCGAGA CGCCAAAGCC ATGCGGTCCC TGCAGCCGCC GGATGGGGCC 
GGCTCGCCCC CCGAGAGTCT GAGGAACGGC TACGTGAAGA GCTGCGTGAG CCCCTTGCGG 
CAGGACCCTC CGCGCGGCTT CTTCTTCCAC CTCTGCCGCT TCTGCAACGT GGAGCTGCGG 
CCGCCGCCGG CCTCTCCCCA GCAGCCGCGG CGCTGCTCCC CCTTCTGCCG GGCGCGCCTC 
TCGCTGGGCG CCCTGGCTGC CTTTGTCCTC GCCCTGCTGC TGGGCGCGGA ACCCGAGAGC 
TGGGCTGCCG GGGCCGCCTG GCTGCGGACG CTGCTGAGCG TGTGTTCGCA CAGCTTGAGC 
CCCCTCTTCA GCATCGCCTG TGCCTTCTTC TTCCTCACCT GCTTCCTCAC CCGGACCAAG 
CGGGGACCCG GCCCGGGCCG GAGCTGCGGC TCCTGGTGGC TGCTGGCGCT GCCCGCCTGC 
TGTTACCTGG GGGACTTCTT GGTGTGGCAG TGGTGGTCTT GGCCTTGGGG GGATGGCGAC 
GCAGGGTCCG CGGCCCCGCA CACGCCCCCG GAGGCGGCAG CGGGCAGGTT GCTGCTGGTG 
CTGAGCTGCG TAGGGCTGCT GCTGACGCTC GCGCACCCGC TGCGGCTCCG GCACTGCGTT 
CTGGTGCTGC TCCTGGCCAG CTTCGTCTGG TGGGTCTCCT TCACCAGCCT CGGGTCGCTG 
CCCTCCGCCC TCAGGCCGCT GCTCTCCGGC CTGGTGGGGG GCGCTGGCTG CCTGCTGGCC 
CTGGGGTTGG ATCACTTCTT TCAAATCAGG GAAGCGCCTC TTCATCCTCG ACTGTCCAGT 
GCCGCCGAAG AAAAAGTGCC TGTGATCCGA CCCCGGAGGA GGTCCAGCTG CGTGTCGTTA 
GGAGAAACTG CAGCCAGTTA CTATGGCAGT TGCAAAATAT TCAGGAGACC GTCGTTGCCT 
TGTATTTCCA GAGAACAGAT GATTCTTTGG GATTGGGACT TAAAACAATG GTATAAGCCT 
CATTATCAAG CTGGAGTGCA GTGGCATGAT CTCGGCTCAC CGCAACCTCC AGCTCCCTGG 
TTCAAGCGAT TCTCCTGCCT CAGCCTCCGG AGTAGCTGGG ATTACAGGCA TGTGCCACCA 
CACCTGGCTA ATTTTGTATT TTTAGTAGAG ATGGGATTTC TCCATGTTGG TCAGGCTGGT 
CTTGAACTCC CGACCTCAGG TGATCTGCCC ACCTCGGCCT CCCAAAGTGC TGGGATTACA 
GGGAATTCTG GAGGTGGAAA TGGAGTTGAT CTTTCAGTGC TAAATGAGGC TCGCAATATG 
GTGTCAGATC TTCTGACTGA TCCAAGCCTT CCACCACAAG TCATTTCCTC TCTACGGAGT 
ATTAGTAGCT TAATGGGTGC TTTCTCAGGT TCCTGTAGGC CAAAGATTAA TCCTCTCACA 
CCATTTCCTG GATTTTACCC CTGTTCTGAA ATAGAGGACC CAGCTGAGAA AGGGGATAGA 
AAACTTAACA AGGGACTAAA TAGGAATAGT TTGCCAACTC CACAGCTGAG GAGAAGCTCA 
GGAACTTCAG GATTGCTACC TGTTGAACAG TCTTCAAGGT GGGATCGTAA TAATGGCAAA 
AGACCTCACC AAGAATTTGG CATTTCAAGT CAAGGATGCT ATCTAAATGG GCCTTTTAAT 
TCAAATCTAC TGACTATCCC GAAGCAAAGG TCATCTTCTG TATCACTGAC TCACCATGTA 
GGTCTCAGAA GAGCTGGTGT TTTGTCCAGT CTGAGTCCTG TGAATTCTTC CAACCATGGA 
CCAGTGTCTA CTGGCTCTCT AACTAATCGA TCACCCATAG AATTTCCTGA TACTGCTGAT 
TTTCTTAATA AGCCAAGCGT TATCTTGCAG AGATCTCTGG GCAATGCACC TAATACTCCA 
GATTTTTATC AGCAACTTAG AAATTCTGAT AGCAATCTGT GTAACAGCTG TGGACATCAA 
ATGCTGAAAT ATGTTTCAAC ATCTGAATCA GATGGTACAG ATTGCTGCAG TGGAAAATCA 
GGTGAAGAAG AAAACATTTT CTCGAAAGAA TCATTCAAAC TTATGGAAAC TCAACAAGAA 
GAGGAAACAG AGAAGAAAGA CAGCAGAAAA TTATTTCAGG AAGGTGATAA GTGGCTAACA 
GAAGAGGCAC AGAGTGAACA GCAAACAAAT ATTGAACAGG AAGTATCACT GGACCTGATT 
TTAGTAGAAG AGTATGACTC ATTAATAGAA AAGATGAGCA ACTGGAATTT TCCAATTTTT 
GAACTTGTAG AAAAGATGGG AGAGAAATCA GGAAGGATTC TCAGTCAGGT TATGTATACC 
TTATTTCAAG ACACTGGTTT ATTGGAAATA TTTAAAATTC CCACTCAACA ATTTATGAAC 
TATTTTCGTG CATTAGAAAA TGGCTATCGA GACATTCCTT ATCACAATCG TATACATGCC 
ACAGATGTGC TACATGCAGT TTGGTATCTG ACAACACGGC CAGTTCCTGG CTTACAGCAG 
ATCCACAATG GTTGTGGAAC AGGAAATGAA ACAGATTCTG ATGGTAGAAT TAACCATGGG 
CGAATTGCTT ATATTTCTTC GAAGAGCTGC TCTAATCCTG ATGAGAGTTA TGGCTGCCTG 
TCTTCAAACA TTCCTGCATT AGAATTGATG GCTCTATACG TGGCAGCTGC CATGCATGAT 
TATGATCACC CAGGGAGGAC AAATGCATTT CTAGTGGCTA CAAATGCCCC TCAGGCAGTT 
TTATACAATG ACAGATCTGT TCTGGAAAAT CATCATGCTG CGTCAGCTTG GAATCTATAT 
CTTTCTCGCC CAGAATACAA CTTCCTTCTT CATCTTGATC ATGTGGAATT CAAGCGCTTT 
CGTTTTTTAG TCATTGAAGC AATCCTTGCT ACGGATCTTA AAAAGCATTT TGATTTTCTC 
GCAGAATTCA ATGCCAAGGC AAATGATGTA AATAGTAATG GCATAGAATG GAGTAATGAA 
AATGATCGCC TCTTGGTATG CCAGGTGTGC ATCAAACTGG CAGATATAAA TGGCCCAGCA 
AAAGTTCGAG ACTTGCATTT GAAATGGACA GAAGGCATTG TCAATGAATT TTATGAGCAG 
GGAGATGAAG AAGCAAATCT TGGTCTGCCC ATCAGTCCAT TCATGGATCG TTCTTCTCCT 
CAACTAGCAA AACTCCAAGA ATCTTTTATC ACCCACATAG TGGGTCCCCT GTGTAACTCC 
TATGATGCTG CTGGTTTGCT ACCAGGTCAG TGGTTAGAAG CAGAAGAGGA TAATGATACT 
GAAAGTGGTG ATGATGAAGA CGGTGAAGAA TTAGATACAG AAGATGAAGA AATGGAAAAC 
AATCTAAATC CAAAACCACC AAGAAGGAAA AGCAGACGGC GAATATTTTG TCAGCTAATG 
CACCACCTCA CTGAAAACCA CAAGATATGG AAGGAAATCG TAGAGGAAGA AGAAAAATGT 
AAAGCTGATG GGAATAAACT GCAGGTGGAG AATTCCTCCT TACCTCAAGC AGATGAGATT 
CAGGTAATTG AAGAGGCAGA TGAAGAGGAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE3B ( XM_006718249.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006718312.1 Homo sapiens phosphodiesterase 3B (PDE3B), transcript variant X3, mRNA.

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CloneID	OHu44898
Clone ID Related Accession (Same CDS sequence)	XM_006718249.3
Accession Version	XM_006718249.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006718249.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGGG ACGAGCGAGA CGCCAAAGCC ATGCGGTCCC TGCAGCCGCC GGATGGGGCC 
GGCTCGCCCC CCGAGAGTCT GAGGAACGGC TACGTGAAGA GCTGCGTGAG CCCCTTGCGG 
CAGGACCCTC CGCGCGGCTT CTTCTTCCAC CTCTGCCGCT TCTGCAACGT GGAGCTGCGG 
CCGCCGCCGG CCTCTCCCCA GCAGCCGCGG CGCTGCTCCC CCTTCTGCCG GGCGCGCCTC 
TCGCTGGGCG CCCTGGCTGC CTTTGTCCTC GCCCTGCTGC TGGGCGCGGA ACCCGAGAGC 
TGGGCTGCCG GGGCCGCCTG GCTGCGGACG CTGCTGAGCG TGTGTTCGCA CAGCTTGAGC 
CCCCTCTTCA GCATCGCCTG TGCCTTCTTC TTCCTCACCT GCTTCCTCAC CCGGACCAAG 
CGGGGACCCG GCCCGGGCCG GAGCTGCGGC TCCTGGTGGC TGCTGGCGCT GCCCGCCTGC 
TGTTACCTGG GGGACTTCTT GGTGTGGCAG TGGTGGTCTT GGCCTTGGGG GGATGGCGAC 
GCAGGGTCCG CGGCCCCGCA CACGCCCCCG GAGGCGGCAG CGGGCAGGTT GCTGCTGGTG 
CTGAGCTGCG TAGGGCTGCT GCTGACGCTC GCGCACCCGC TGCGGCTCCG GCACTGCGTT 
CTGGTGCTGC TCCTGGCCAG CTTCGTCTGG TGGGTCTCCT TCACCAGCCT CGGGTCGCTG 
CCCTCCGCCC TCAGGCCGCT GCTCTCCGGC CTGGTGGGGG GCGCTGGCTG CCTGCTGGCC 
CTGGGGTTGG ATCACTTCTT TCAAATCAGG GAAGCGCCTC TTCATCCTCG ACTGTCCAGT 
GCCGCCGAAG AAAAAGTGCC TGTGATCCGA CCCCGGAGGA GGTCCAGCTG CGTGTCGTTA 
GGAGAAACTG CAGCCAGTTA CTATGGCAGT TGCAAAATAT TCAGGAGACC GTCGTTGCCT 
TGTATTTCCA GAGAACAGAT GATTCTTTGG GATTGGGACT TAAAACAATG GTATAAGCCT 
CATTATCAAA ATTCTGGAGG TGGAAATGGA GTTGATCTTT CAGTGCTAAA TGAGGCTCGC 
AATATGGTGT CAGATCTTCT GACTGATCCA AGCCTTCCAC CACAAGTCAT TTCCTCTCTA 
CGGAGTATTA GTAGCTTAAT GGGTGCTTTC TCAGGTTCCT GTAGGCCAAA GATTAATCCT 
CTCACACCAT TTCCTGGATT TTACCCCTGT TCTGAAATAG AGGACCCAGC TGAGAAAGGG 
GATAGAAAAC TTAACAAGGG ACTAAATAGG AATAGTTTGC CAACTCCACA GCTGAGGAGA 
AGCTCAGGAA CTTCAGGATT GCTACCTGTT GAACAGTCTT CAAGGTGGGA TCGTAATAAT 
GGCAAAAGAC CTCACCAAGA ATTTGGCATT TCAAGTCAAG GATGCTATCT AAATGGGCCT 
TTTAATTCAA ATCTACTGAC TATCCCGAAG CAAAGGTCAT CTTCTGTATC ACTGACTCAC 
CATGTAGGTC TCAGAAGAGC TGGTGTTTTG TCCAGTCTGA GTCCTGTGAA TTCTTCCAAC 
CATGGACCAG TGTCTACTGG CTCTCTAACT AATCGATCAC CCATAGAATT TCCTGATACT 
GCTGATTTTC TTAATAAGCC AAGCGTTATC TTGCAGAGAT CTCTGGGCAA TGCACCTAAT 
ACTCCAGATT TTTATCAGCA ACTTAGAAAT TCTGATAGCA ATCTGTGTAA CAGCTGTGGA 
CATCAAATGC TGAAATATGT TTCAACATCT GAATCAGATG GTACAGATTG CTGCAGTGGA 
AAATCAGGTG AAGAAGAAAA CATTTTCTCG AAAGAATCAT TCAAACTTAT GGAAACTCAA 
CAAGAAGAGG AAACAGAGAA GAAAGACAGC AGAAAATTAT TTCAGGAAGG TGATAAGTGG 
CTAACAGAAG AGGCACAGAG TGAACAGCAA ACAAATATTG AACAGGAAGT ATCACTGGAC 
CTGATTTTAG TAGAAGAGTA TGACTCATTA ATAGAAAAGA TGAGCAACTG GAATTTTCCA 
ATTTTTGAAC TTGTAGAAAA GATGGGAGAG AAATCAGGAA GGATTCTCAG TCAGGTTATG 
TATACCTTAT TTCAAGACAC TGGTTTATTG GAAATATTTA AAATTCCCAC TCAACAATTT 
ATGAACTATT TTCGTGCATT AGAAAATGGC TATCGAGACA TTCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006718312.1
CDS	386..2593
Translation	MRRDERDAKAMRSLQPPDGAGSPPESLRNGYVKSCVSPLRQDPPRGFFFHLCRFCNVELRPPPASPQQPRRCSPFCRARLSLGALAAFVLALLLGAEPESWAAGAAWLRTLLSVCSHSLSPLFSIACAFFFLTCFLTRTKRGPGPGRSCGSWWLLALPACCYLGDFLVWQWWSWPWGDGDAGSAAPHTPPEAAAGRLLLVLSCVGLLLTLAHPLRLRHCVLVLLLASFVWWVSFTSLGSLPSALRPLLSGLVGGAGCLLALGLDHFFQIREAPLHPRLSSAAEEKVPVIRPRRRSSCVSLGETAASYYGSCKIFRRPSLPCISREQMILWDWDLKQWYKPHYQNSGGGNGVDLSVLNEARNMVSDLLTDPSLPPQVISSLRSISSLMGAFSGSCRPKINPLTPFPGFYPCSEIEDPAEKGDRKLNKGLNRNSLPTPQLRRSSGTSGLLPVEQSSRWDRNNGKRPHQEFGISSQGCYLNGPFNSNLLTIPKQRSSSVSLTHHVGLRRAGVLSSLSPVNSSNHGPVSTGSLTNRSPIEFPDTADFLNKPSVILQRSLGNAPNTPDFYQQLRNSDSNLCNSCGHQMLKYVSTSESDGTDCCSGKSGEEENIFSKESFKLMETQQEEETEKKDSRKLFQEGDKWLTEEAQSEQQTNIEQEVSLDLILVEEYDSLIEKMSNWNFPIFELVEKMGEKSGRILSQVMYTLFQDTGLLEIFKIPTQQFMNYFRALENGYRDIP

Target ORF information:

RefSeq Version	XM_006718249.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphodiesterase 3B (PDE3B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006718249.3

ORF Insert Sequence:

ATGAGGAGGG ACGAGCGAGA CGCCAAAGCC ATGCGGTCCC TGCAGCCGCC GGATGGGGCC 
GGCTCGCCCC CCGAGAGTCT GAGGAACGGC TACGTGAAGA GCTGCGTGAG CCCCTTGCGG 
CAGGACCCTC CGCGCGGCTT CTTCTTCCAC CTCTGCCGCT TCTGCAACGT GGAGCTGCGG 
CCGCCGCCGG CCTCTCCCCA GCAGCCGCGG CGCTGCTCCC CCTTCTGCCG GGCGCGCCTC 
TCGCTGGGCG CCCTGGCTGC CTTTGTCCTC GCCCTGCTGC TGGGCGCGGA ACCCGAGAGC 
TGGGCTGCCG GGGCCGCCTG GCTGCGGACG CTGCTGAGCG TGTGTTCGCA CAGCTTGAGC 
CCCCTCTTCA GCATCGCCTG TGCCTTCTTC TTCCTCACCT GCTTCCTCAC CCGGACCAAG 
CGGGGACCCG GCCCGGGCCG GAGCTGCGGC TCCTGGTGGC TGCTGGCGCT GCCCGCCTGC 
TGTTACCTGG GGGACTTCTT GGTGTGGCAG TGGTGGTCTT GGCCTTGGGG GGATGGCGAC 
GCAGGGTCCG CGGCCCCGCA CACGCCCCCG GAGGCGGCAG CGGGCAGGTT GCTGCTGGTG 
CTGAGCTGCG TAGGGCTGCT GCTGACGCTC GCGCACCCGC TGCGGCTCCG GCACTGCGTT 
CTGGTGCTGC TCCTGGCCAG CTTCGTCTGG TGGGTCTCCT TCACCAGCCT CGGGTCGCTG 
CCCTCCGCCC TCAGGCCGCT GCTCTCCGGC CTGGTGGGGG GCGCTGGCTG CCTGCTGGCC 
CTGGGGTTGG ATCACTTCTT TCAAATCAGG GAAGCGCCTC TTCATCCTCG ACTGTCCAGT 
GCCGCCGAAG AAAAAGTGCC TGTGATCCGA CCCCGGAGGA GGTCCAGCTG CGTGTCGTTA 
GGAGAAACTG CAGCCAGTTA CTATGGCAGT TGCAAAATAT TCAGGAGACC GTCGTTGCCT 
TGTATTTCCA GAGAACAGAT GATTCTTTGG GATTGGGACT TAAAACAATG GTATAAGCCT 
CATTATCAAA ATTCTGGAGG TGGAAATGGA GTTGATCTTT CAGTGCTAAA TGAGGCTCGC 
AATATGGTGT CAGATCTTCT GACTGATCCA AGCCTTCCAC CACAAGTCAT TTCCTCTCTA 
CGGAGTATTA GTAGCTTAAT GGGTGCTTTC TCAGGTTCCT GTAGGCCAAA GATTAATCCT 
CTCACACCAT TTCCTGGATT TTACCCCTGT TCTGAAATAG AGGACCCAGC TGAGAAAGGG 
GATAGAAAAC TTAACAAGGG ACTAAATAGG AATAGTTTGC CAACTCCACA GCTGAGGAGA 
AGCTCAGGAA CTTCAGGATT GCTACCTGTT GAACAGTCTT CAAGGTGGGA TCGTAATAAT 
GGCAAAAGAC CTCACCAAGA ATTTGGCATT TCAAGTCAAG GATGCTATCT AAATGGGCCT 
TTTAATTCAA ATCTACTGAC TATCCCGAAG CAAAGGTCAT CTTCTGTATC ACTGACTCAC 
CATGTAGGTC TCAGAAGAGC TGGTGTTTTG TCCAGTCTGA GTCCTGTGAA TTCTTCCAAC 
CATGGACCAG TGTCTACTGG CTCTCTAACT AATCGATCAC CCATAGAATT TCCTGATACT 
GCTGATTTTC TTAATAAGCC AAGCGTTATC TTGCAGAGAT CTCTGGGCAA TGCACCTAAT 
ACTCCAGATT TTTATCAGCA ACTTAGAAAT TCTGATAGCA ATCTGTGTAA CAGCTGTGGA 
CATCAAATGC TGAAATATGT TTCAACATCT GAATCAGATG GTACAGATTG CTGCAGTGGA 
AAATCAGGTG AAGAAGAAAA CATTTTCTCG AAAGAATCAT TCAAACTTAT GGAAACTCAA 
CAAGAAGAGG AAACAGAGAA GAAAGACAGC AGAAAATTAT TTCAGGAAGG TGATAAGTGG 
CTAACAGAAG AGGCACAGAG TGAACAGCAA ACAAATATTG AACAGGAAGT ATCACTGGAC 
CTGATTTTAG TAGAAGAGTA TGACTCATTA ATAGAAAAGA TGAGCAACTG GAATTTTCCA 
ATTTTTGAAC TTGTAGAAAA GATGGGAGAG AAATCAGGAA GGATTCTCAG TCAGGTTATG 
TATACCTTAT TTCAAGACAC TGGTTTATTG GAAATATTTA AAATTCCCAC TCAACAATTT 
ATGAACTATT TTCGTGCATT AGAAAATGGC TATCGAGACA TTCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE3B ( XM_017017911.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873400.1 Homo sapiens phosphodiesterase 3B (PDE3B), transcript variant X1, mRNA.

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CloneID	OHu92310
Clone ID Related Accession (Same CDS sequence)	XM_017017911.1 , XM_017017911.2
Accession Version	XM_017017911.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGGG ACGAGCGAGA CGCCAAAGCC ATGCGGTCCC TGCAGCCGCC GGATGGGGCC 
GGCTCGCCCC CCGAGAGTCT GAGGAACGGC TACGTGAAGA GCTGCGTGAG CCCCTTGCGG 
CAGGACCCTC CGCGCGGCTT CTTCTTCCAC CTCTGCCGCT TCTGCAACGT GGAGCTGCGG 
CCGCCGCCGG CCTCTCCCCA GCAGCCGCGG CGCTGCTCCC CCTTCTGCCG GGCGCGCCTC 
TCGCTGGGCG CCCTGGCTGC CTTTGTCCTC GCCCTGCTGC TGGGCGCGGA ACCCGAGAGC 
TGGGCTGCCG GGGCCGCCTG GCTGCGGACG CTGCTGAGCG TGTGTTCGCA CAGCTTGAGC 
CCCCTCTTCA GCATCGCCTG TGCCTTCTTC TTCCTCACCT GCTTCCTCAC CCGGACCAAG 
CGGGGACCCG GCCCGGGCCG GAGCTGCGGC TCCTGGTGGC TGCTGGCGCT GCCCGCCTGC 
TGTTACCTGG GGGACTTCTT GGTGTGGCAG TGGTGGTCTT GGCCTTGGGG GGATGGCGAC 
GCAGGGTCCG CGGCCCCGCA CACGCCCCCG GAGGCGGCAG CGGGCAGGTT GCTGCTGGTG 
CTGAGCTGCG TAGGGCTGCT GCTGACGCTC GCGCACCCGC TGCGGCTCCG GCACTGCGTT 
CTGGTGCTGC TCCTGGCCAG CTTCGTCTGG TGGGTCTCCT TCACCAGCCT CGGGTCGCTG 
CCCTCCGCCC TCAGGCCGCT GCTCTCCGGC CTGGTGGGGG GCGCTGGCTG CCTGCTGGCC 
CTGGGGTTGG ATCACTTCTT TCAAATCAGG GAAGCGCCTC TTCATCCTCG ACTGTCCAGT 
GCCGCCGAAG AAAAAGTGCC TGTGATCCGA CCCCGGAGGA GGTCCAGCTG CGTGTCGTTA 
GGAGAAACTG CAGCCAGTTA CTATGGCAGT TGCAAAATAT TCAGGAGACC GTCGTTGCCT 
TGTATTTCCA GAGAACAGAT GATTCTTTGG GATTGGGACT TAAAACAATG GTATAAGCCT 
CATTATCAAA ATTCTGGAGG TGGAAATGGA GTTGATCTTT CAGTGCTAAA TGAGGCTCGC 
AATATGGTGT CAGATCTTCT GACTGATCCA AGCCTTCCAC CACAAGTCAT TTCCTCTCTA 
CGGAGTATTA GTAGCTTAAT GGGTGCTTTC TCAGGTTCCT GTAGGCCAAA GATTAATCCT 
CTCACACCAT TTCCTGGATT TTACCCCTGT TCTGAAATAG AGGACCCAGC TGAGAAAGGG 
GATAGAAAAC TTAACAAGGG ACTAAATAGG AATAGTTTGC CAACTCCACA GCTGAGGAGA 
AGCTCAGGAA CTTCAGGATT GCTACCTGTT GAACAGTCTT CAAGGTGGGA TCGTAATAAT 
GGCAAAAGAC CTCACCAAGA ATTTGGCATT TCAAGTCAAG GATGCTATCT AAATGGGCCT 
TTTAATTCAA ATCTACTGAC TATCCCGAAG CAAAGGTCAT CTTCTGTATC ACTGACTCAC 
CATGTAGGTC TCAGAAGAGC TGGTGTTTTG TCCAGTCTGA GTCCTGTGAA TTCTTCCAAC 
CATGGACCAG TGTCTACTGG CTCTCTAACT AATCGATCAC CCATAGAATT TCCTGATACT 
GCTGATTTTC TTAATAAGCC AAGCGTTATC TTGCAGAGAT CTCTGGGCAA TGCACCTAAT 
ACTCCAGATT TTTATCAGCA ACTTAGAAAT TCTGATAGCA ATCTGTGTAA CAGCTGTGGA 
CATCAAATGC TGAAATATGT TTCAACATCT GAATCAGATG GTACAGATTG CTGCAGTGGA 
AAATCAGGTG AAGAAGAAAA CATTTTCTCG AAAGAATCAT TCAAACTTAT GGAAACTCAA 
CAAGAAGAGG AAACAGAGAA GAAAGACAGC AGAAAATTAT TTCAGGAAGG TGATAAGTGG 
CTAACAGAAG AGGCACAGAG TGAACAGCAA ACAAATATTG AACAGGAAGT ATCACTGGAC 
CTGATTTTAG TAGAAGAGTA TGACTCATTA ATAGAAAAGA TGAGCAACTG GAATTTTCCA 
ATTTTTGAAC TTGTAGAAAA GATGGGAGAG AAATCAGGAA GGATTCTCAG TCAGGTTATG 
TATACCTTAT TTCAAGACAC TGGTTTATTG GAAATATTTA AAATTCCCAC TCAACAATTT 
ATGAACTATT TTCGTGCATT AGAAAATGGC TATCGAGACA TTCCTTATTC TGATGGTAGA 
ATTAACCATG GGCGAATTGC TTATATTTCT TCGAAGAGCT GCTCTAATCC TGATGAGAGT 
TATGGCTGCC TGTCTTCAAA CATTCCTGCA TTAGAATTGA TGGCTCTATA CGTGGCAGCT 
GCCATGCATG ATTATGATCA CCCAGGGAGG ACAAATGCAT TTCTAGTGGC TACAAATGCC 
CCTCAGGCAG TTTTATACAA TGACAGATCT GTTCTGGAAA ATCATCATGC TGCGTCAGCT 
TGGAATCTAT ATCTTTCTCG CCCAGAATAC AACTTCCTTC TTCATCTTGA TCATGTGGAA 
TTCAAGCGCT TTCGTTTTTT AGTCATTGAA GCAATCCTTG CTACGGATCT TAAAAAGCAT 
TTTGATTTTC TCGCAGAATT CAATGCCAAG GCAAATGATG TAAATAGTAA TGGCATAGAA 
TGGAGTAATG AAAATGATCG CCTCTTGGTA TGCCAGGTGT GCATCAAACT GGCAGATATA 
AATGGCCCAG CAAAAGTTCG AGACTTGCAT TTGAAATGGA CAGAAGGCAT TGTCAATGAA 
TTTTATGAGC AGGGAGATGA AGAAGCAAAT CTTGGTCTGC CCATCAGTCC ATTCATGGAT 
CGTTCTTCTC CTCAACTAGC AAAACTCCAA GAATCTTTTA TCACCCACAT AGTGGGTCCC 
CTGTGTAACT CCTATGATGC TGCTGGTTTG CTACCAGGTC AGTGGTTAGA AGCAGAAGAG 
GATAATGATA CTGAAAGTGG TGATGATGAA GACGGTGAAG AATTAGATAC AGAAGATGAA 
GAAATGGAAA ACAATCTAAA TCCAAAACCA CCAAGAAGGA AAAGCAGACG GCGAATATTT 
TGTCAGCTAA TGCACCACCT CACTGAAAAC CACAAGATAT GGAAGGAAAT CGTAGAGGAA 
GAAGAAAAAT GTAAAGCTGA TGGGAATAAA CTGCAGGTGG AGAATTCCTC CTTACCTCAA 
GCAGATGAGA TTCAGGTAAT TGAAGAGGCA GATGAAGAGG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873400.1
CDS	386..3610
Translation	MRRDERDAKAMRSLQPPDGAGSPPESLRNGYVKSCVSPLRQDPPRGFFFHLCRFCNVELRPPPASPQQPRRCSPFCRARLSLGALAAFVLALLLGAEPESWAAGAAWLRTLLSVCSHSLSPLFSIACAFFFLTCFLTRTKRGPGPGRSCGSWWLLALPACCYLGDFLVWQWWSWPWGDGDAGSAAPHTPPEAAAGRLLLVLSCVGLLLTLAHPLRLRHCVLVLLLASFVWWVSFTSLGSLPSALRPLLSGLVGGAGCLLALGLDHFFQIREAPLHPRLSSAAEEKVPVIRPRRRSSCVSLGETAASYYGSCKIFRRPSLPCISREQMILWDWDLKQWYKPHYQNSGGGNGVDLSVLNEARNMVSDLLTDPSLPPQVISSLRSISSLMGAFSGSCRPKINPLTPFPGFYPCSEIEDPAEKGDRKLNKGLNRNSLPTPQLRRSSGTSGLLPVEQSSRWDRNNGKRPHQEFGISSQGCYLNGPFNSNLLTIPKQRSSSVSLTHHVGLRRAGVLSSLSPVNSSNHGPVSTGSLTNRSPIEFPDTADFLNKPSVILQRSLGNAPNTPDFYQQLRNSDSNLCNSCGHQMLKYVSTSESDGTDCCSGKSGEEENIFSKESFKLMETQQEEETEKKDSRKLFQEGDKWLTEEAQSEQQTNIEQEVSLDLILVEEYDSLIEKMSNWNFPIFELVEKMGEKSGRILSQVMYTLFQDTGLLEIFKIPTQQFMNYFRALENGYRDIPYSDGRINHGRIAYISSKSCSNPDESYGCLSSNIPALELMALYVAAAMHDYDHPGRTNAFLVATNAPQAVLYNDRSVLENHHAASAWNLYLSRPEYNFLLHLDHVEFKRFRFLVIEAILATDLKKHFDFLAEFNAKANDVNSNGIEWSNENDRLLVCQVCIKLADINGPAKVRDLHLKWTEGIVNEFYEQGDEEANLGLPISPFMDRSSPQLAKLQESFITHIVGPLCNSYDAAGLLPGQWLEAEEDNDTESGDDEDGEELDTEDEEMENNLNPKPPRRKSRRRIFCQLMHHLTENHKIWKEIVEEEEKCKADGNKLQVENSSLPQADEIQVIEEADEEE

Target ORF information:

RefSeq Version	XM_017017911.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphodiesterase 3B (PDE3B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017017911.1

ORF Insert Sequence:

ATGAGGAGGG ACGAGCGAGA CGCCAAAGCC ATGCGGTCCC TGCAGCCGCC GGATGGGGCC 
GGCTCGCCCC CCGAGAGTCT GAGGAACGGC TACGTGAAGA GCTGCGTGAG CCCCTTGCGG 
CAGGACCCTC CGCGCGGCTT CTTCTTCCAC CTCTGCCGCT TCTGCAACGT GGAGCTGCGG 
CCGCCGCCGG CCTCTCCCCA GCAGCCGCGG CGCTGCTCCC CCTTCTGCCG GGCGCGCCTC 
TCGCTGGGCG CCCTGGCTGC CTTTGTCCTC GCCCTGCTGC TGGGCGCGGA ACCCGAGAGC 
TGGGCTGCCG GGGCCGCCTG GCTGCGGACG CTGCTGAGCG TGTGTTCGCA CAGCTTGAGC 
CCCCTCTTCA GCATCGCCTG TGCCTTCTTC TTCCTCACCT GCTTCCTCAC CCGGACCAAG 
CGGGGACCCG GCCCGGGCCG GAGCTGCGGC TCCTGGTGGC TGCTGGCGCT GCCCGCCTGC 
TGTTACCTGG GGGACTTCTT GGTGTGGCAG TGGTGGTCTT GGCCTTGGGG GGATGGCGAC 
GCAGGGTCCG CGGCCCCGCA CACGCCCCCG GAGGCGGCAG CGGGCAGGTT GCTGCTGGTG 
CTGAGCTGCG TAGGGCTGCT GCTGACGCTC GCGCACCCGC TGCGGCTCCG GCACTGCGTT 
CTGGTGCTGC TCCTGGCCAG CTTCGTCTGG TGGGTCTCCT TCACCAGCCT CGGGTCGCTG 
CCCTCCGCCC TCAGGCCGCT GCTCTCCGGC CTGGTGGGGG GCGCTGGCTG CCTGCTGGCC 
CTGGGGTTGG ATCACTTCTT TCAAATCAGG GAAGCGCCTC TTCATCCTCG ACTGTCCAGT 
GCCGCCGAAG AAAAAGTGCC TGTGATCCGA CCCCGGAGGA GGTCCAGCTG CGTGTCGTTA 
GGAGAAACTG CAGCCAGTTA CTATGGCAGT TGCAAAATAT TCAGGAGACC GTCGTTGCCT 
TGTATTTCCA GAGAACAGAT GATTCTTTGG GATTGGGACT TAAAACAATG GTATAAGCCT 
CATTATCAAA ATTCTGGAGG TGGAAATGGA GTTGATCTTT CAGTGCTAAA TGAGGCTCGC 
AATATGGTGT CAGATCTTCT GACTGATCCA AGCCTTCCAC CACAAGTCAT TTCCTCTCTA 
CGGAGTATTA GTAGCTTAAT GGGTGCTTTC TCAGGTTCCT GTAGGCCAAA GATTAATCCT 
CTCACACCAT TTCCTGGATT TTACCCCTGT TCTGAAATAG AGGACCCAGC TGAGAAAGGG 
GATAGAAAAC TTAACAAGGG ACTAAATAGG AATAGTTTGC CAACTCCACA GCTGAGGAGA 
AGCTCAGGAA CTTCAGGATT GCTACCTGTT GAACAGTCTT CAAGGTGGGA TCGTAATAAT 
GGCAAAAGAC CTCACCAAGA ATTTGGCATT TCAAGTCAAG GATGCTATCT AAATGGGCCT 
TTTAATTCAA ATCTACTGAC TATCCCGAAG CAAAGGTCAT CTTCTGTATC ACTGACTCAC 
CATGTAGGTC TCAGAAGAGC TGGTGTTTTG TCCAGTCTGA GTCCTGTGAA TTCTTCCAAC 
CATGGACCAG TGTCTACTGG CTCTCTAACT AATCGATCAC CCATAGAATT TCCTGATACT 
GCTGATTTTC TTAATAAGCC AAGCGTTATC TTGCAGAGAT CTCTGGGCAA TGCACCTAAT 
ACTCCAGATT TTTATCAGCA ACTTAGAAAT TCTGATAGCA ATCTGTGTAA CAGCTGTGGA 
CATCAAATGC TGAAATATGT TTCAACATCT GAATCAGATG GTACAGATTG CTGCAGTGGA 
AAATCAGGTG AAGAAGAAAA CATTTTCTCG AAAGAATCAT TCAAACTTAT GGAAACTCAA 
CAAGAAGAGG AAACAGAGAA GAAAGACAGC AGAAAATTAT TTCAGGAAGG TGATAAGTGG 
CTAACAGAAG AGGCACAGAG TGAACAGCAA ACAAATATTG AACAGGAAGT ATCACTGGAC 
CTGATTTTAG TAGAAGAGTA TGACTCATTA ATAGAAAAGA TGAGCAACTG GAATTTTCCA 
ATTTTTGAAC TTGTAGAAAA GATGGGAGAG AAATCAGGAA GGATTCTCAG TCAGGTTATG 
TATACCTTAT TTCAAGACAC TGGTTTATTG GAAATATTTA AAATTCCCAC TCAACAATTT 
ATGAACTATT TTCGTGCATT AGAAAATGGC TATCGAGACA TTCCTTATTC TGATGGTAGA 
ATTAACCATG GGCGAATTGC TTATATTTCT TCGAAGAGCT GCTCTAATCC TGATGAGAGT 
TATGGCTGCC TGTCTTCAAA CATTCCTGCA TTAGAATTGA TGGCTCTATA CGTGGCAGCT 
GCCATGCATG ATTATGATCA CCCAGGGAGG ACAAATGCAT TTCTAGTGGC TACAAATGCC 
CCTCAGGCAG TTTTATACAA TGACAGATCT GTTCTGGAAA ATCATCATGC TGCGTCAGCT 
TGGAATCTAT ATCTTTCTCG CCCAGAATAC AACTTCCTTC TTCATCTTGA TCATGTGGAA 
TTCAAGCGCT TTCGTTTTTT AGTCATTGAA GCAATCCTTG CTACGGATCT TAAAAAGCAT 
TTTGATTTTC TCGCAGAATT CAATGCCAAG GCAAATGATG TAAATAGTAA TGGCATAGAA 
TGGAGTAATG AAAATGATCG CCTCTTGGTA TGCCAGGTGT GCATCAAACT GGCAGATATA 
AATGGCCCAG CAAAAGTTCG AGACTTGCAT TTGAAATGGA CAGAAGGCAT TGTCAATGAA 
TTTTATGAGC AGGGAGATGA AGAAGCAAAT CTTGGTCTGC CCATCAGTCC ATTCATGGAT 
CGTTCTTCTC CTCAACTAGC AAAACTCCAA GAATCTTTTA TCACCCACAT AGTGGGTCCC 
CTGTGTAACT CCTATGATGC TGCTGGTTTG CTACCAGGTC AGTGGTTAGA AGCAGAAGAG 
GATAATGATA CTGAAAGTGG TGATGATGAA GACGGTGAAG AATTAGATAC AGAAGATGAA 
GAAATGGAAA ACAATCTAAA TCCAAAACCA CCAAGAAGGA AAAGCAGACG GCGAATATTT 
TGTCAGCTAA TGCACCACCT CACTGAAAAC CACAAGATAT GGAAGGAAAT CGTAGAGGAA 
GAAGAAAAAT GTAAAGCTGA TGGGAATAAA CTGCAGGTGG AGAATTCCTC CTTACCTCAA 
GCAGATGAGA TTCAGGTAAT TGAAGAGGCA GATGAAGAGG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE3B ( XM_017017911.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873400.1 Homo sapiens phosphodiesterase 3B (PDE3B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu92310
Clone ID Related Accession (Same CDS sequence)	XM_017017911.1 , XM_017017911.2
Accession Version	XM_017017911.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017017911.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGGG ACGAGCGAGA CGCCAAAGCC ATGCGGTCCC TGCAGCCGCC GGATGGGGCC 
GGCTCGCCCC CCGAGAGTCT GAGGAACGGC TACGTGAAGA GCTGCGTGAG CCCCTTGCGG 
CAGGACCCTC CGCGCGGCTT CTTCTTCCAC CTCTGCCGCT TCTGCAACGT GGAGCTGCGG 
CCGCCGCCGG CCTCTCCCCA GCAGCCGCGG CGCTGCTCCC CCTTCTGCCG GGCGCGCCTC 
TCGCTGGGCG CCCTGGCTGC CTTTGTCCTC GCCCTGCTGC TGGGCGCGGA ACCCGAGAGC 
TGGGCTGCCG GGGCCGCCTG GCTGCGGACG CTGCTGAGCG TGTGTTCGCA CAGCTTGAGC 
CCCCTCTTCA GCATCGCCTG TGCCTTCTTC TTCCTCACCT GCTTCCTCAC CCGGACCAAG 
CGGGGACCCG GCCCGGGCCG GAGCTGCGGC TCCTGGTGGC TGCTGGCGCT GCCCGCCTGC 
TGTTACCTGG GGGACTTCTT GGTGTGGCAG TGGTGGTCTT GGCCTTGGGG GGATGGCGAC 
GCAGGGTCCG CGGCCCCGCA CACGCCCCCG GAGGCGGCAG CGGGCAGGTT GCTGCTGGTG 
CTGAGCTGCG TAGGGCTGCT GCTGACGCTC GCGCACCCGC TGCGGCTCCG GCACTGCGTT 
CTGGTGCTGC TCCTGGCCAG CTTCGTCTGG TGGGTCTCCT TCACCAGCCT CGGGTCGCTG 
CCCTCCGCCC TCAGGCCGCT GCTCTCCGGC CTGGTGGGGG GCGCTGGCTG CCTGCTGGCC 
CTGGGGTTGG ATCACTTCTT TCAAATCAGG GAAGCGCCTC TTCATCCTCG ACTGTCCAGT 
GCCGCCGAAG AAAAAGTGCC TGTGATCCGA CCCCGGAGGA GGTCCAGCTG CGTGTCGTTA 
GGAGAAACTG CAGCCAGTTA CTATGGCAGT TGCAAAATAT TCAGGAGACC GTCGTTGCCT 
TGTATTTCCA GAGAACAGAT GATTCTTTGG GATTGGGACT TAAAACAATG GTATAAGCCT 
CATTATCAAA ATTCTGGAGG TGGAAATGGA GTTGATCTTT CAGTGCTAAA TGAGGCTCGC 
AATATGGTGT CAGATCTTCT GACTGATCCA AGCCTTCCAC CACAAGTCAT TTCCTCTCTA 
CGGAGTATTA GTAGCTTAAT GGGTGCTTTC TCAGGTTCCT GTAGGCCAAA GATTAATCCT 
CTCACACCAT TTCCTGGATT TTACCCCTGT TCTGAAATAG AGGACCCAGC TGAGAAAGGG 
GATAGAAAAC TTAACAAGGG ACTAAATAGG AATAGTTTGC CAACTCCACA GCTGAGGAGA 
AGCTCAGGAA CTTCAGGATT GCTACCTGTT GAACAGTCTT CAAGGTGGGA TCGTAATAAT 
GGCAAAAGAC CTCACCAAGA ATTTGGCATT TCAAGTCAAG GATGCTATCT AAATGGGCCT 
TTTAATTCAA ATCTACTGAC TATCCCGAAG CAAAGGTCAT CTTCTGTATC ACTGACTCAC 
CATGTAGGTC TCAGAAGAGC TGGTGTTTTG TCCAGTCTGA GTCCTGTGAA TTCTTCCAAC 
CATGGACCAG TGTCTACTGG CTCTCTAACT AATCGATCAC CCATAGAATT TCCTGATACT 
GCTGATTTTC TTAATAAGCC AAGCGTTATC TTGCAGAGAT CTCTGGGCAA TGCACCTAAT 
ACTCCAGATT TTTATCAGCA ACTTAGAAAT TCTGATAGCA ATCTGTGTAA CAGCTGTGGA 
CATCAAATGC TGAAATATGT TTCAACATCT GAATCAGATG GTACAGATTG CTGCAGTGGA 
AAATCAGGTG AAGAAGAAAA CATTTTCTCG AAAGAATCAT TCAAACTTAT GGAAACTCAA 
CAAGAAGAGG AAACAGAGAA GAAAGACAGC AGAAAATTAT TTCAGGAAGG TGATAAGTGG 
CTAACAGAAG AGGCACAGAG TGAACAGCAA ACAAATATTG AACAGGAAGT ATCACTGGAC 
CTGATTTTAG TAGAAGAGTA TGACTCATTA ATAGAAAAGA TGAGCAACTG GAATTTTCCA 
ATTTTTGAAC TTGTAGAAAA GATGGGAGAG AAATCAGGAA GGATTCTCAG TCAGGTTATG 
TATACCTTAT TTCAAGACAC TGGTTTATTG GAAATATTTA AAATTCCCAC TCAACAATTT 
ATGAACTATT TTCGTGCATT AGAAAATGGC TATCGAGACA TTCCTTATTC TGATGGTAGA 
ATTAACCATG GGCGAATTGC TTATATTTCT TCGAAGAGCT GCTCTAATCC TGATGAGAGT 
TATGGCTGCC TGTCTTCAAA CATTCCTGCA TTAGAATTGA TGGCTCTATA CGTGGCAGCT 
GCCATGCATG ATTATGATCA CCCAGGGAGG ACAAATGCAT TTCTAGTGGC TACAAATGCC 
CCTCAGGCAG TTTTATACAA TGACAGATCT GTTCTGGAAA ATCATCATGC TGCGTCAGCT 
TGGAATCTAT ATCTTTCTCG CCCAGAATAC AACTTCCTTC TTCATCTTGA TCATGTGGAA 
TTCAAGCGCT TTCGTTTTTT AGTCATTGAA GCAATCCTTG CTACGGATCT TAAAAAGCAT 
TTTGATTTTC TCGCAGAATT CAATGCCAAG GCAAATGATG TAAATAGTAA TGGCATAGAA 
TGGAGTAATG AAAATGATCG CCTCTTGGTA TGCCAGGTGT GCATCAAACT GGCAGATATA 
AATGGCCCAG CAAAAGTTCG AGACTTGCAT TTGAAATGGA CAGAAGGCAT TGTCAATGAA 
TTTTATGAGC AGGGAGATGA AGAAGCAAAT CTTGGTCTGC CCATCAGTCC ATTCATGGAT 
CGTTCTTCTC CTCAACTAGC AAAACTCCAA GAATCTTTTA TCACCCACAT AGTGGGTCCC 
CTGTGTAACT CCTATGATGC TGCTGGTTTG CTACCAGGTC AGTGGTTAGA AGCAGAAGAG 
GATAATGATA CTGAAAGTGG TGATGATGAA GACGGTGAAG AATTAGATAC AGAAGATGAA 
GAAATGGAAA ACAATCTAAA TCCAAAACCA CCAAGAAGGA AAAGCAGACG GCGAATATTT 
TGTCAGCTAA TGCACCACCT CACTGAAAAC CACAAGATAT GGAAGGAAAT CGTAGAGGAA 
GAAGAAAAAT GTAAAGCTGA TGGGAATAAA CTGCAGGTGG AGAATTCCTC CTTACCTCAA 
GCAGATGAGA TTCAGGTAAT TGAAGAGGCA GATGAAGAGG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873400.1
CDS	386..3610
Translation	MRRDERDAKAMRSLQPPDGAGSPPESLRNGYVKSCVSPLRQDPPRGFFFHLCRFCNVELRPPPASPQQPRRCSPFCRARLSLGALAAFVLALLLGAEPESWAAGAAWLRTLLSVCSHSLSPLFSIACAFFFLTCFLTRTKRGPGPGRSCGSWWLLALPACCYLGDFLVWQWWSWPWGDGDAGSAAPHTPPEAAAGRLLLVLSCVGLLLTLAHPLRLRHCVLVLLLASFVWWVSFTSLGSLPSALRPLLSGLVGGAGCLLALGLDHFFQIREAPLHPRLSSAAEEKVPVIRPRRRSSCVSLGETAASYYGSCKIFRRPSLPCISREQMILWDWDLKQWYKPHYQNSGGGNGVDLSVLNEARNMVSDLLTDPSLPPQVISSLRSISSLMGAFSGSCRPKINPLTPFPGFYPCSEIEDPAEKGDRKLNKGLNRNSLPTPQLRRSSGTSGLLPVEQSSRWDRNNGKRPHQEFGISSQGCYLNGPFNSNLLTIPKQRSSSVSLTHHVGLRRAGVLSSLSPVNSSNHGPVSTGSLTNRSPIEFPDTADFLNKPSVILQRSLGNAPNTPDFYQQLRNSDSNLCNSCGHQMLKYVSTSESDGTDCCSGKSGEEENIFSKESFKLMETQQEEETEKKDSRKLFQEGDKWLTEEAQSEQQTNIEQEVSLDLILVEEYDSLIEKMSNWNFPIFELVEKMGEKSGRILSQVMYTLFQDTGLLEIFKIPTQQFMNYFRALENGYRDIPYSDGRINHGRIAYISSKSCSNPDESYGCLSSNIPALELMALYVAAAMHDYDHPGRTNAFLVATNAPQAVLYNDRSVLENHHAASAWNLYLSRPEYNFLLHLDHVEFKRFRFLVIEAILATDLKKHFDFLAEFNAKANDVNSNGIEWSNENDRLLVCQVCIKLADINGPAKVRDLHLKWTEGIVNEFYEQGDEEANLGLPISPFMDRSSPQLAKLQESFITHIVGPLCNSYDAAGLLPGQWLEAEEDNDTESGDDEDGEELDTEDEEMENNLNPKPPRRKSRRRIFCQLMHHLTENHKIWKEIVEEEEKCKADGNKLQVENSSLPQADEIQVIEEADEEE

Target ORF information:

RefSeq Version	XM_017017911.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphodiesterase 3B (PDE3B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017017911.2

ORF Insert Sequence:

ATGAGGAGGG ACGAGCGAGA CGCCAAAGCC ATGCGGTCCC TGCAGCCGCC GGATGGGGCC 
GGCTCGCCCC CCGAGAGTCT GAGGAACGGC TACGTGAAGA GCTGCGTGAG CCCCTTGCGG 
CAGGACCCTC CGCGCGGCTT CTTCTTCCAC CTCTGCCGCT TCTGCAACGT GGAGCTGCGG 
CCGCCGCCGG CCTCTCCCCA GCAGCCGCGG CGCTGCTCCC CCTTCTGCCG GGCGCGCCTC 
TCGCTGGGCG CCCTGGCTGC CTTTGTCCTC GCCCTGCTGC TGGGCGCGGA ACCCGAGAGC 
TGGGCTGCCG GGGCCGCCTG GCTGCGGACG CTGCTGAGCG TGTGTTCGCA CAGCTTGAGC 
CCCCTCTTCA GCATCGCCTG TGCCTTCTTC TTCCTCACCT GCTTCCTCAC CCGGACCAAG 
CGGGGACCCG GCCCGGGCCG GAGCTGCGGC TCCTGGTGGC TGCTGGCGCT GCCCGCCTGC 
TGTTACCTGG GGGACTTCTT GGTGTGGCAG TGGTGGTCTT GGCCTTGGGG GGATGGCGAC 
GCAGGGTCCG CGGCCCCGCA CACGCCCCCG GAGGCGGCAG CGGGCAGGTT GCTGCTGGTG 
CTGAGCTGCG TAGGGCTGCT GCTGACGCTC GCGCACCCGC TGCGGCTCCG GCACTGCGTT 
CTGGTGCTGC TCCTGGCCAG CTTCGTCTGG TGGGTCTCCT TCACCAGCCT CGGGTCGCTG 
CCCTCCGCCC TCAGGCCGCT GCTCTCCGGC CTGGTGGGGG GCGCTGGCTG CCTGCTGGCC 
CTGGGGTTGG ATCACTTCTT TCAAATCAGG GAAGCGCCTC TTCATCCTCG ACTGTCCAGT 
GCCGCCGAAG AAAAAGTGCC TGTGATCCGA CCCCGGAGGA GGTCCAGCTG CGTGTCGTTA 
GGAGAAACTG CAGCCAGTTA CTATGGCAGT TGCAAAATAT TCAGGAGACC GTCGTTGCCT 
TGTATTTCCA GAGAACAGAT GATTCTTTGG GATTGGGACT TAAAACAATG GTATAAGCCT 
CATTATCAAA ATTCTGGAGG TGGAAATGGA GTTGATCTTT CAGTGCTAAA TGAGGCTCGC 
AATATGGTGT CAGATCTTCT GACTGATCCA AGCCTTCCAC CACAAGTCAT TTCCTCTCTA 
CGGAGTATTA GTAGCTTAAT GGGTGCTTTC TCAGGTTCCT GTAGGCCAAA GATTAATCCT 
CTCACACCAT TTCCTGGATT TTACCCCTGT TCTGAAATAG AGGACCCAGC TGAGAAAGGG 
GATAGAAAAC TTAACAAGGG ACTAAATAGG AATAGTTTGC CAACTCCACA GCTGAGGAGA 
AGCTCAGGAA CTTCAGGATT GCTACCTGTT GAACAGTCTT CAAGGTGGGA TCGTAATAAT 
GGCAAAAGAC CTCACCAAGA ATTTGGCATT TCAAGTCAAG GATGCTATCT AAATGGGCCT 
TTTAATTCAA ATCTACTGAC TATCCCGAAG CAAAGGTCAT CTTCTGTATC ACTGACTCAC 
CATGTAGGTC TCAGAAGAGC TGGTGTTTTG TCCAGTCTGA GTCCTGTGAA TTCTTCCAAC 
CATGGACCAG TGTCTACTGG CTCTCTAACT AATCGATCAC CCATAGAATT TCCTGATACT 
GCTGATTTTC TTAATAAGCC AAGCGTTATC TTGCAGAGAT CTCTGGGCAA TGCACCTAAT 
ACTCCAGATT TTTATCAGCA ACTTAGAAAT TCTGATAGCA ATCTGTGTAA CAGCTGTGGA 
CATCAAATGC TGAAATATGT TTCAACATCT GAATCAGATG GTACAGATTG CTGCAGTGGA 
AAATCAGGTG AAGAAGAAAA CATTTTCTCG AAAGAATCAT TCAAACTTAT GGAAACTCAA 
CAAGAAGAGG AAACAGAGAA GAAAGACAGC AGAAAATTAT TTCAGGAAGG TGATAAGTGG 
CTAACAGAAG AGGCACAGAG TGAACAGCAA ACAAATATTG AACAGGAAGT ATCACTGGAC 
CTGATTTTAG TAGAAGAGTA TGACTCATTA ATAGAAAAGA TGAGCAACTG GAATTTTCCA 
ATTTTTGAAC TTGTAGAAAA GATGGGAGAG AAATCAGGAA GGATTCTCAG TCAGGTTATG 
TATACCTTAT TTCAAGACAC TGGTTTATTG GAAATATTTA AAATTCCCAC TCAACAATTT 
ATGAACTATT TTCGTGCATT AGAAAATGGC TATCGAGACA TTCCTTATTC TGATGGTAGA 
ATTAACCATG GGCGAATTGC TTATATTTCT TCGAAGAGCT GCTCTAATCC TGATGAGAGT 
TATGGCTGCC TGTCTTCAAA CATTCCTGCA TTAGAATTGA TGGCTCTATA CGTGGCAGCT 
GCCATGCATG ATTATGATCA CCCAGGGAGG ACAAATGCAT TTCTAGTGGC TACAAATGCC 
CCTCAGGCAG TTTTATACAA TGACAGATCT GTTCTGGAAA ATCATCATGC TGCGTCAGCT 
TGGAATCTAT ATCTTTCTCG CCCAGAATAC AACTTCCTTC TTCATCTTGA TCATGTGGAA 
TTCAAGCGCT TTCGTTTTTT AGTCATTGAA GCAATCCTTG CTACGGATCT TAAAAAGCAT 
TTTGATTTTC TCGCAGAATT CAATGCCAAG GCAAATGATG TAAATAGTAA TGGCATAGAA 
TGGAGTAATG AAAATGATCG CCTCTTGGTA TGCCAGGTGT GCATCAAACT GGCAGATATA 
AATGGCCCAG CAAAAGTTCG AGACTTGCAT TTGAAATGGA CAGAAGGCAT TGTCAATGAA 
TTTTATGAGC AGGGAGATGA AGAAGCAAAT CTTGGTCTGC CCATCAGTCC ATTCATGGAT 
CGTTCTTCTC CTCAACTAGC AAAACTCCAA GAATCTTTTA TCACCCACAT AGTGGGTCCC 
CTGTGTAACT CCTATGATGC TGCTGGTTTG CTACCAGGTC AGTGGTTAGA AGCAGAAGAG 
GATAATGATA CTGAAAGTGG TGATGATGAA GACGGTGAAG AATTAGATAC AGAAGATGAA 
GAAATGGAAA ACAATCTAAA TCCAAAACCA CCAAGAAGGA AAAGCAGACG GCGAATATTT 
TGTCAGCTAA TGCACCACCT CACTGAAAAC CACAAGATAT GGAAGGAAAT CGTAGAGGAA 
GAAGAAAAAT GTAAAGCTGA TGGGAATAAA CTGCAGGTGG AGAATTCCTC CTTACCTCAA 
GCAGATGAGA TTCAGGTAAT TGAAGAGGCA GATGAAGAGG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE3B ( XM_017017912.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873401.1 Homo sapiens phosphodiesterase 3B (PDE3B), transcript variant X2, mRNA.

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CloneID	OHu92311
Clone ID Related Accession (Same CDS sequence)	XM_017017912.1
Accession Version	XM_017017912.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGGG ACGAGCGAGA CGCCAAAGCC ATGCGGTCCC TGCAGCCGCC GGATGGGGCC 
GGCTCGCCCC CCGAGAGTCT GAGGAACGGC TACGTGAAGA GCTGCGTGAG CCCCTTGCGG 
CAGGACCCTC CGCGCGGCTT CTTCTTCCAC CTCTGCCGCT TCTGCAACGT GGAGCTGCGG 
CCGCCGCCGG CCTCTCCCCA GCAGCCGCGG CGCTGCTCCC CCTTCTGCCG GGCGCGCCTC 
TCGCTGGGCG CCCTGGCTGC CTTTGTCCTC GCCCTGCTGC TGGGCGCGGA ACCCGAGAGC 
TGGGCTGCCG GGGCCGCCTG GCTGCGGACG CTGCTGAGCG TGTGTTCGCA CAGCTTGAGC 
CCCCTCTTCA GCATCGCCTG TGCCTTCTTC TTCCTCACCT GCTTCCTCAC CCGGACCAAG 
CGGGGACCCG GCCCGGGCCG GAGCTGCGGC TCCTGGTGGC TGCTGGCGCT GCCCGCCTGC 
TGTTACCTGG GGGACTTCTT GGTGTGGCAG TGGTGGTCTT GGCCTTGGGG GGATGGCGAC 
GCAGGGTCCG CGGCCCCGCA CACGCCCCCG GAGGCGGCAG CGGGCAGGTT GCTGCTGGTG 
CTGAGCTGCG TAGGGCTGCT GCTGACGCTC GCGCACCCGC TGCGGCTCCG GCACTGCGTT 
CTGGTGCTGC TCCTGGCCAG CTTCGTCTGG TGGGTCTCCT TCACCAGCCT CGGGTCGCTG 
CCCTCCGCCC TCAGGCCGCT GCTCTCCGGC CTGGTGGGGG GCGCTGGCTG CCTGCTGGCC 
CTGGGGTTGG ATCACTTCTT TCAAATCAGG GAAGCGCCTC TTCATCCTCG ACTGTCCAGT 
GCCGCCGAAG AAAAAGTGCC TGTGATCCGA CCCCGGAGGA GGTCCAGCTG CGTGTCGTTA 
GGAGAAACTG CAGCCAGTTA CTATGGCAGT TGCAAAATAT TCAGGAGACC GTCGTTGCCT 
TGTATTTCCA GAGAACAGAT GATTCTTTGG GATTGGGACT TAAAACAATG GTATAAGCCT 
CATTATCAAA ATTCTGGAGG TGGAAATGGA GTTGATCTTT CAGTGCTAAA TGAGGCTCGC 
AATATGGTGT CAGATCTTCT GACTGATCCA AGCCTTCCAC CACAAGTCAT TTCCTCTCTA 
CGGAGTATTA GTAGCTTAAT GGGTGCTTTC TCAGGTTCCT GTAGGCCAAA GATTAATCCT 
CTCACACCAT TTCCTGGATT TTACCCCTGT TCTGAAATAG AGGACCCAGC TGAGAAAGGG 
GATAGAAAAC TTAACAAGGG ACTAAATAGG AATAGTTTGC CAACTCCACA GCTGAGGAGA 
AGCTCAGGAA CTTCAGGATT GCTACCTGTT GAACAGTCTT CAAGGTGGGA TCGTAATAAT 
GGCAAAAGAC CTCACCAAGA ATTTGGCATT TCAAGTCAAG GATGCTATCT AAATGGGCCT 
TTTAATTCAA ATCTACTGAC TATCCCGAAG CAAAGGTCAT CTTCTGTATC ACTGACTCAC 
CATGTAGGTC TCAGAAGAGC TGGTGTTTTG TCCAGTCTGA GTCCTGTGAA TTCTTCCAAC 
CATGGACCAG TGTCTACTGG CTCTCTAACT AATCGATCAC CCATAGAATT TCCTGATACT 
GCTGATTTTC TTAATAAGCC AAGCGTTATC TTGCAGAGAT CTCTGGGCAA TGCACCTAAT 
ACTCCAGATT TTTATCAGCA ACTTAGAAAT TCTGATAGCA ATCTGTGTAA CAGCTGTGGA 
CATCAAATGC TGAAATATGT TTCAACATCT GAATCAGATG GTACAGATTG CTGCAGTGGA 
AAATCAGGTG AAGAAGAAAA CATTTTCTCG AAAGAATCAT TCAAACTTAT GGAAACTCAA 
CAAGAAGAGG AAACAGAGAA GAAAGACAGC AGAAAATTAT TTCAGGAAGG TGATAAGTGG 
CTAACAGAAG AGGCACAGAG TGAACAGCAA ACAAATATTG AACAGGAAGT ATCACTGGAC 
CTGATTTTAG TAGAAGAGTA TGACTCATTA ATAGAAAAGA TGAGCAACTG GAATTTTCCA 
ATTTTTGAAC TTGTAGAAAA GATGGGAGAG AAATCAGGAA GGATTCTCAG TCAGGTTATG 
TATACCTTAT TTCAAGACAC TGGTTTATTG GAAATATTTA AAATTCCCAC TCAACAATTT 
ATGAACTATT TTCGTGCATT AGAAAATGGC TATCGAGACA TTCCTTATCA CAATCGTATA 
CATGCCACAG ATGTGCTACA TGCAGTTTGG TATCTGACAA CACGGCCAGT TCCTGGCTTA 
CAGCAGATCC ACAATGGTTG TGGAACAGGA AATGAAACAG ATTCTGATGG TAGAATTAAC 
CATGGGCGAA TTGCTTATAT TTCTTCGAAG AGCTGCTCTA ATCCTGATGA GAGTTATGGC 
TGCCTGTCTT CAAACATTCC TGCATTAGAA TTGATGGCTC TATACGTGGC AGCTGCCATG 
CATGATTATG ATCACCCAGG GAGGACAAAT GCATTTCTAG TGGCTACAAA TGCCCCTCAG 
GCAGTTTTAT ACAATGACAG ATCTGTTCTG GAAAATCATC ATGCTGCGTC AGCTTGGAAT 
CTATATCTTT CTCGCCCAGA ATACAACTTC CTTCTTCATC TTGATCATGT GGAATTCAAG 
CGCTTTCGTT TTTTAGTCAT TGAAGCAATC CTTGCTACGG ATCTTAAAAA GCATTTTGAT 
TTTCTCGCAG AATTCAATGC CAAGGCAAAT GATGTAAATA GTAATGGCAT AGAATGGAGT 
AATGAAAATG ATCGCCTCTT GGTATGCCAG GTGTGCATCA AACTGGCAGA TATAAATGGC 
CCAGCAAAAG TTCGAGACTT GCATTTGAAA TGGACAGAAG GCATTGTCAA TGAATTTTAT 
GAGCAGGGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873401.1
CDS	386..3277
Translation	MRRDERDAKAMRSLQPPDGAGSPPESLRNGYVKSCVSPLRQDPPRGFFFHLCRFCNVELRPPPASPQQPRRCSPFCRARLSLGALAAFVLALLLGAEPESWAAGAAWLRTLLSVCSHSLSPLFSIACAFFFLTCFLTRTKRGPGPGRSCGSWWLLALPACCYLGDFLVWQWWSWPWGDGDAGSAAPHTPPEAAAGRLLLVLSCVGLLLTLAHPLRLRHCVLVLLLASFVWWVSFTSLGSLPSALRPLLSGLVGGAGCLLALGLDHFFQIREAPLHPRLSSAAEEKVPVIRPRRRSSCVSLGETAASYYGSCKIFRRPSLPCISREQMILWDWDLKQWYKPHYQNSGGGNGVDLSVLNEARNMVSDLLTDPSLPPQVISSLRSISSLMGAFSGSCRPKINPLTPFPGFYPCSEIEDPAEKGDRKLNKGLNRNSLPTPQLRRSSGTSGLLPVEQSSRWDRNNGKRPHQEFGISSQGCYLNGPFNSNLLTIPKQRSSSVSLTHHVGLRRAGVLSSLSPVNSSNHGPVSTGSLTNRSPIEFPDTADFLNKPSVILQRSLGNAPNTPDFYQQLRNSDSNLCNSCGHQMLKYVSTSESDGTDCCSGKSGEEENIFSKESFKLMETQQEEETEKKDSRKLFQEGDKWLTEEAQSEQQTNIEQEVSLDLILVEEYDSLIEKMSNWNFPIFELVEKMGEKSGRILSQVMYTLFQDTGLLEIFKIPTQQFMNYFRALENGYRDIPYHNRIHATDVLHAVWYLTTRPVPGLQQIHNGCGTGNETDSDGRINHGRIAYISSKSCSNPDESYGCLSSNIPALELMALYVAAAMHDYDHPGRTNAFLVATNAPQAVLYNDRSVLENHHAASAWNLYLSRPEYNFLLHLDHVEFKRFRFLVIEAILATDLKKHFDFLAEFNAKANDVNSNGIEWSNENDRLLVCQVCIKLADINGPAKVRDLHLKWTEGIVNEFYEQG

Target ORF information:

RefSeq Version	XM_017017912.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphodiesterase 3B (PDE3B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017017912.1

ORF Insert Sequence:

ATGAGGAGGG ACGAGCGAGA CGCCAAAGCC ATGCGGTCCC TGCAGCCGCC GGATGGGGCC 
GGCTCGCCCC CCGAGAGTCT GAGGAACGGC TACGTGAAGA GCTGCGTGAG CCCCTTGCGG 
CAGGACCCTC CGCGCGGCTT CTTCTTCCAC CTCTGCCGCT TCTGCAACGT GGAGCTGCGG 
CCGCCGCCGG CCTCTCCCCA GCAGCCGCGG CGCTGCTCCC CCTTCTGCCG GGCGCGCCTC 
TCGCTGGGCG CCCTGGCTGC CTTTGTCCTC GCCCTGCTGC TGGGCGCGGA ACCCGAGAGC 
TGGGCTGCCG GGGCCGCCTG GCTGCGGACG CTGCTGAGCG TGTGTTCGCA CAGCTTGAGC 
CCCCTCTTCA GCATCGCCTG TGCCTTCTTC TTCCTCACCT GCTTCCTCAC CCGGACCAAG 
CGGGGACCCG GCCCGGGCCG GAGCTGCGGC TCCTGGTGGC TGCTGGCGCT GCCCGCCTGC 
TGTTACCTGG GGGACTTCTT GGTGTGGCAG TGGTGGTCTT GGCCTTGGGG GGATGGCGAC 
GCAGGGTCCG CGGCCCCGCA CACGCCCCCG GAGGCGGCAG CGGGCAGGTT GCTGCTGGTG 
CTGAGCTGCG TAGGGCTGCT GCTGACGCTC GCGCACCCGC TGCGGCTCCG GCACTGCGTT 
CTGGTGCTGC TCCTGGCCAG CTTCGTCTGG TGGGTCTCCT TCACCAGCCT CGGGTCGCTG 
CCCTCCGCCC TCAGGCCGCT GCTCTCCGGC CTGGTGGGGG GCGCTGGCTG CCTGCTGGCC 
CTGGGGTTGG ATCACTTCTT TCAAATCAGG GAAGCGCCTC TTCATCCTCG ACTGTCCAGT 
GCCGCCGAAG AAAAAGTGCC TGTGATCCGA CCCCGGAGGA GGTCCAGCTG CGTGTCGTTA 
GGAGAAACTG CAGCCAGTTA CTATGGCAGT TGCAAAATAT TCAGGAGACC GTCGTTGCCT 
TGTATTTCCA GAGAACAGAT GATTCTTTGG GATTGGGACT TAAAACAATG GTATAAGCCT 
CATTATCAAA ATTCTGGAGG TGGAAATGGA GTTGATCTTT CAGTGCTAAA TGAGGCTCGC 
AATATGGTGT CAGATCTTCT GACTGATCCA AGCCTTCCAC CACAAGTCAT TTCCTCTCTA 
CGGAGTATTA GTAGCTTAAT GGGTGCTTTC TCAGGTTCCT GTAGGCCAAA GATTAATCCT 
CTCACACCAT TTCCTGGATT TTACCCCTGT TCTGAAATAG AGGACCCAGC TGAGAAAGGG 
GATAGAAAAC TTAACAAGGG ACTAAATAGG AATAGTTTGC CAACTCCACA GCTGAGGAGA 
AGCTCAGGAA CTTCAGGATT GCTACCTGTT GAACAGTCTT CAAGGTGGGA TCGTAATAAT 
GGCAAAAGAC CTCACCAAGA ATTTGGCATT TCAAGTCAAG GATGCTATCT AAATGGGCCT 
TTTAATTCAA ATCTACTGAC TATCCCGAAG CAAAGGTCAT CTTCTGTATC ACTGACTCAC 
CATGTAGGTC TCAGAAGAGC TGGTGTTTTG TCCAGTCTGA GTCCTGTGAA TTCTTCCAAC 
CATGGACCAG TGTCTACTGG CTCTCTAACT AATCGATCAC CCATAGAATT TCCTGATACT 
GCTGATTTTC TTAATAAGCC AAGCGTTATC TTGCAGAGAT CTCTGGGCAA TGCACCTAAT 
ACTCCAGATT TTTATCAGCA ACTTAGAAAT TCTGATAGCA ATCTGTGTAA CAGCTGTGGA 
CATCAAATGC TGAAATATGT TTCAACATCT GAATCAGATG GTACAGATTG CTGCAGTGGA 
AAATCAGGTG AAGAAGAAAA CATTTTCTCG AAAGAATCAT TCAAACTTAT GGAAACTCAA 
CAAGAAGAGG AAACAGAGAA GAAAGACAGC AGAAAATTAT TTCAGGAAGG TGATAAGTGG 
CTAACAGAAG AGGCACAGAG TGAACAGCAA ACAAATATTG AACAGGAAGT ATCACTGGAC 
CTGATTTTAG TAGAAGAGTA TGACTCATTA ATAGAAAAGA TGAGCAACTG GAATTTTCCA 
ATTTTTGAAC TTGTAGAAAA GATGGGAGAG AAATCAGGAA GGATTCTCAG TCAGGTTATG 
TATACCTTAT TTCAAGACAC TGGTTTATTG GAAATATTTA AAATTCCCAC TCAACAATTT 
ATGAACTATT TTCGTGCATT AGAAAATGGC TATCGAGACA TTCCTTATCA CAATCGTATA 
CATGCCACAG ATGTGCTACA TGCAGTTTGG TATCTGACAA CACGGCCAGT TCCTGGCTTA 
CAGCAGATCC ACAATGGTTG TGGAACAGGA AATGAAACAG ATTCTGATGG TAGAATTAAC 
CATGGGCGAA TTGCTTATAT TTCTTCGAAG AGCTGCTCTA ATCCTGATGA GAGTTATGGC 
TGCCTGTCTT CAAACATTCC TGCATTAGAA TTGATGGCTC TATACGTGGC AGCTGCCATG 
CATGATTATG ATCACCCAGG GAGGACAAAT GCATTTCTAG TGGCTACAAA TGCCCCTCAG 
GCAGTTTTAT ACAATGACAG ATCTGTTCTG GAAAATCATC ATGCTGCGTC AGCTTGGAAT 
CTATATCTTT CTCGCCCAGA ATACAACTTC CTTCTTCATC TTGATCATGT GGAATTCAAG 
CGCTTTCGTT TTTTAGTCAT TGAAGCAATC CTTGCTACGG ATCTTAAAAA GCATTTTGAT 
TTTCTCGCAG AATTCAATGC CAAGGCAAAT GATGTAAATA GTAATGGCAT AGAATGGAGT 
AATGAAAATG ATCGCCTCTT GGTATGCCAG GTGTGCATCA AACTGGCAGA TATAAATGGC 
CCAGCAAAAG TTCGAGACTT GCATTTGAAA TGGACAGAAG GCATTGTCAA TGAATTTTAT 
GAGCAGGGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Clinical effects of phosphodiesterase 3A mutations in inherited hypertension with brachydactyly.
Hypertension (Dallas, Tex. : 1979)66(4)800-8(2015 Oct)
Toka O,Tank J,Sch?chterle C,Aydin A,Maass PG,Elitok S,Bartels-Klein E,Hollfinger I,Lindschau C,Mai K,Boschmann M,Rahn G,Movsesian MA,M?ller T,Doescher A,Gnoth S,M?hl A,Toka HR,Wefeld-Neuenfeld Y,Utz W,T?pper A,Jordan J,Schulz-Menger J,Klussmann E,B?hring S,Luft FC

Genome-wide association study of vitamin D levels in children: replication in the Western Australian Pregnancy Cohort (Raine) study.
Genes and immunity15(8)578-83(2014 Dec)
Anderson D,Holt BJ,Pennell CE,Holt PG,Hart PH,Blackwell JM

Novel loci for adiponectin levels and their influence on type 2 diabetes and metabolic traits: a multi-ethnic meta-analysis of 45,891 individuals.
PLoS genetics8(3)e1002607(2012)
Dastani Z,Hivert MF,Timpson N,Perry JR,Yuan X,Scott RA,Henneman P,Heid IM,Kizer JR,Lyytik?inen LP,Fuchsberger C,Tanaka T,Morris AP,Small K,Isaacs A,Beekman M,Coassin S,Lohman K,Qi L,Kanoni S,Pankow JS,Uh HW,Wu Y,Bidulescu A,Rasmussen-Torvik LJ,Greenwood CM,Ladouceur M,Grimsby J,Manning AK,Liu CT,Kooner J,Mooser VE,Vollenweider P,Kapur KA,Chambers J,Wareham NJ,Langenberg C,Frants R,Willems-Vandijk K,Oostra BA,Willems SM,Lamina C,Winkler TW,Psaty BM,Tracy RP,Brody J,Chen I,Viikari J,K?h?nen M,Pramstaller PP,Evans DM,St Pourcain B,Sattar N,Wood AR,Bandinelli S,Carlson OD,Egan JM,B?hringer S,van Heemst D,Kedenko L,Kristiansson K,Nuotio ML,Loo BM,Harris T,Garcia M,Kanaya A,Haun M,Klopp N,Wichmann HE,Deloukas P,Katsareli E,Couper DJ,Duncan BB,Kloppenburg M,Adair LS,Borja JB,,,,,Wilson JG,Musani S,Guo X,Johnson T,Semple R,Teslovich TM,Allison MA,Redline S,Buxbaum SG,Mohlke KL,Meulenbelt I,Ballantyne CM,Dedoussis GV,Hu FB,Liu Y,Paulweber B,Spector TD,Slagboom PE,Ferrucci L,Jula A,Perola M,Raitakari O,Florez JC,Salomaa V,Eriksson JG,Frayling TM,Hicks AA,Lehtim?ki T,Smith GD,Siscovick DS,Kronenberg F,van Duijn C,Loos RJ,Waterworth DM,Meigs JB,Dupuis J,Richards JB,Voight BF,Scott LJ,Steinthorsdottir V,Dina C,Welch RP,Zeggini E,Huth C,Aulchenko YS,Thorleifsson G,McCulloch LJ,Ferreira T,Grallert H,Amin N,Wu G,Willer CJ,Raychaudhuri S,McCarroll SA,Hofmann OM,Segr? AV,van Hoek M,Navarro P,Ardlie K,Balkau B,Benediktsson R,Bennett AJ,Blagieva R,Boerwinkle E,Bonnycastle LL,Bostr?m KB,Bravenboer B,Bumpstead S,Burtt NP,Charpentier G,Chines PS,Cornelis M,Crawford G,Doney AS,Elliott KS,Elliott AL,Erdos MR,Fox CS,Franklin CS,Ganser M,Gieger C,Grarup N,Green T,Griffin S,Groves CJ,Guiducci C,Hadjadj S,Hassanali N,Herder C,Isomaa B,Jackson AU,Johnson PR,J?rgensen T,Kao WH,Kong A,Kraft P,Kuusisto J,Lauritzen T,Li M,Lieverse A,Lindgren CM,Lyssenko V,Marre M,Meitinger T,Midthjell K,Morken MA,Narisu N,Nilsson P,Owen KR,Payne F,Petersen AK,Platou C,Proen?a C,Prokopenko I,Rathmann W,Rayner NW,Robertson NR,Rocheleau G,Roden M,Sampson MJ,Saxena R,Shields BM,Shrader P,Sigurdsson G,Spars? T,Strassburger K,Stringham HM,Sun Q,Swift AJ,Thorand B,Tichet J,Tuomi T,van Dam RM,van Haeften TW,van Herpt T,van Vliet-Ostaptchouk JV,Walters GB,Weedon MN,Wijmenga C,Witteman J,Bergman RN,Cauchi S,Collins FS,Gloyn AL,Gyllensten U,Hansen T,Hide WA,Hitman GA,Hofman A,Hunter DJ,Hveem K,Laakso M,Morris AD,Palmer CN,Rudan I,Sijbrands E,Stein LD,Tuomilehto J,Uitterlinden A,Walker M,Watanabe RM,Abecasis GR,Boehm BO,Campbell H,Daly MJ,Hattersley AT,Pedersen O,Barroso I,Groop L,Sladek R,Thorsteinsdottir U,Wilson JF,Illig T,Froguel P,van Duijn CM,Stefansson K,Altshuler D,Boehnke M,McCarthy MI,Soranzo N,Wheeler E,Glazer NL,Bouatia-Naji N,M?gi R,Randall J,Elliott P,Rybin D,Dehghan A,Hottenga JJ,Song K,Goel A,Lajunen T,Doney A,Cavalcanti-Proen?a C,Kumari M,Timpson NJ,Zabena C,Ingelsson E,An P,O'Connell J,Luan J,Elliott A,McCarroll SA,Roccasecca RM,Pattou F,Sethupathy P,Ariyurek Y,Barter P,Beilby JP,Ben-Shlomo Y,Bergmann S,Bochud M,Bonnefond A,Borch-Johnsen K,B?ttcher Y,Brunner E,Bumpstead SJ,Chen YD,Chines P,Clarke R,Coin LJ,Cooper MN,Crisponi L,Day IN,de Geus EJ,Delplanque J,Fedson AC,Fischer-Rosinsky A,Forouhi NG,Franzosi MG,Galan P,Goodarzi MO,Graessler J,Grundy S,Gwilliam R,Hallmans G,Hammond N,Han X,Hartikainen AL,Hayward C,Heath SC,Hercberg S,Hillman DR,Hingorani AD,Hui J,Hung J,Kaakinen M,Kaprio J,Kesaniemi YA,Kivimaki M,Knight B,Koskinen S,Kovacs P,Kyvik KO,Lathrop GM,Lawlor DA,Le Bacquer O,Lecoeur C,Li Y,Mahley R,Mangino M,Mart?nez-Larrad MT,McAteer JB,McPherson R,Meisinger C,Melzer D,Meyre D,Mitchell BD,Mukherjee S,Naitza S,Neville MJ,Orr? M,Pakyz R,Paolisso G,Pattaro C,Pearson D,Peden JF,Pedersen NL,Pfeiffer AF,Pichler I,Polasek O,Posthuma D,Potter SC,Pouta A,Province MA,Rayner NW,Rice K,Ripatti S,Rivadeneira F,Rolandsson O,Sandbaek A,Sandhu M,Sanna S,Sayer AA,Scheet P,Seedorf U,Sharp SJ,Shields B,Sigur?sson G,Sijbrands EJ,Silveira A,Simpson L,Singleton A,Smith NL,Sovio U,Swift A,Syddall H,Syv?nen AC,T?njes A,Uitterlinden AG,van Dijk KW,Varma D,Visvikis-Siest S,Vitart V,Vogelzangs N,Waeber G,Wagner PJ,Walley A,Ward KL,Watkins H,Wild SH,Willemsen G,Witteman JC,Yarnell JW,Zelenika D,Zethelius B,Zhai G,Zhao JH,Zillikens MC,,,,Borecki IB,Meneton P,Magnusson PK,Nathan DM,Williams GH,Silander K,Bornstein SR,Schwarz P,Spranger J,Karpe F,Shuldiner AR,Cooper C,Serrano-R?os M,Lind L,Palmer LJ,Hu FB 1st,Franks PW,Ebrahim S,Marmot M,Kao WH,Pramstaller PP,Wright AF,Stumvoll M,Hamsten A,,Buchanan TA,Valle TT,Rotter JI,Penninx BW,Boomsma DI,Cao A,Scuteri A,Schlessinger D,Uda M,Ruokonen A,Jarvelin MR,Peltonen L,Mooser V,Sladek R,,,Musunuru K,Smith AV,Edmondson AC,Stylianou IM,Koseki M,Pirruccello JP,Chasman DI,Johansen CT,Fouchier SW,Peloso GM,Barbalic M,Ricketts SL,Bis JC,Feitosa MF,Orho-Melander M,Melander O,Li X,Li M,Cho YS,Go MJ,Kim YJ,Lee JY,Park T,Kim K,Sim X,Ong RT,Croteau-Chonka DC,Lange LA,Smith JD,Ziegler A,Zhang W,Zee RY,Whitfield JB,Thompson JR,Surakka I,Spector TD,Smit JH,Sinisalo J,Scott J,Saharinen J,Sabatti C,Rose LM,Roberts R,Rieder M,Parker AN,Pare G,O'Donnell CJ,Nieminen MS,Nickerson DA,Montgomery GW,McArdle W,Masson D,Martin NG,Marroni F,Lucas G,Luben R,Lokki ML,Lettre G,Launer LJ,Lakatta EG,Laaksonen R,Kyvik KO,K?nig IR,Khaw KT,Kaplan LM,Johansson ?,Janssens AC,Igl W,Hovingh GK,Hengstenberg C,Havulinna AS,Hastie ND,Harris TB,Haritunians T,Hall AS,Groop LC,Gonzalez E,Freimer NB,Erdmann J,Ejebe KG,D?ring A,Dominiczak AF,Demissie S,Deloukas P,de Faire U,Crawford G,Chen YD,Caulfield MJ,Boekholdt SM,Assimes TL,Quertermous T,Seielstad M,Wong TY,Tai ES,Feranil AB,Kuzawa CW,Taylor HA Jr,Gabriel SB,Holm H,Gudnason V,Krauss RM,Ordovas JM,Munroe PB,Kooner JS,Tall AR,Hegele RA,Kastelein JJ,Schadt EE,Strachan DP,Reilly MP,Samani NJ,Schunkert H,Cupples LA,Sandhu MS,Ridker PM,Rader DJ,Kathiresan S

From PDE3B to the regulation of energy homeostasis.
Current opinion in pharmacology11(6)676-82(2011 Dec)
Degerman E,Ahmad F,Chung YW,Guirguis E,Omar B,Stenson L,Manganiello V

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PDE3B cDNA ORF clone, Homo sapiens(human)

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gRNAs

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ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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ORF Insert Sequence:

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