ACSL5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Gene Symbol	ACSL5
Entrez Gene ID	51703
Full Name	acyl-CoA synthetase long chain family member 5
Synonyms	ACS2,ACS5,FACL5
General protein information	Preferred Names acyl-CoA synthetase long chain family member 5 Names long-chain-fatty-acid--CoA ligase 5 FACL5 for fatty acid coenzyme A ligase 5 LACS 5 fatty acid coenzyme A ligase 5 fatty-acid-Coenzyme A ligase, long-chain 5 long-chain acyl-CoA synthetase 5 long-chain fatty acid coenzyme A ligase 5
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q25.2
Summary	The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in uterus and spleen, and in trace amounts in normal brain, but has markedly increased levels in malignant gliomas. This gene functions in mediating fatty acid-induced glioma cell growth. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	605677

mRNA and Protein(s)

mRNA	Protein	Name
NM_203379.1	NP_976313.1	long-chain-fatty-acid--CoA ligase 5 isoform b
XM_017016324.2	XP_016871813.1	long-chain-fatty-acid--CoA ligase 5 isoform X1
NM_203380.1	NP_976314.1	long-chain-fatty-acid--CoA ligase 5 isoform b
XM_017016324.1	XP_016871813.1	long-chain-fatty-acid--CoA ligase 5 isoform X1
NM_016234.3	NP_057318.2	long-chain-fatty-acid--CoA ligase 5 isoform a
NM_203379.2	NP_976313.1	long-chain-fatty-acid--CoA ligase 5 isoform b

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG3961	NP_730369.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011603	XP_320900.3
Arabidopsis thaliana (thale cress)	LACS6	NP_566265.1
Oryza sativa (rice)	Os11g0147000	NP_001065739.1
Oryza sativa (rice)	Os12g0143900	NP_001066139.1
Canis lupus familiaris (dog)	ACSL5	XP_005637786.1
Mus musculus (house mouse)	Acsl5	NP_082252.1
Homo sapiens (human)	ACSL5	NP_057318.2
Bos taurus (cattle)	ACSL5	NP_001069118.1
Danio rerio (zebrafish)	acsl5	NP_001004599.1
Caenorhabditis elegans (roundworm)	acs-13	NP_490744.1
Pan troglodytes (chimpanzee)	ACSL5	XP_001146649.1
Rattus norvegicus (Norway rat)	Acsl5	NP_446059.1
Gallus gallus (chicken)	ACSL5	NP_001026408.1
Xenopus tropicalis (tropical clawed frog)	acsl5	NP_001011069.1

Gene Ontology
Bibliography

Related articles in PubMed

Association of long-chain acyl-coenzyme A synthetase?5 expression in human breast cancer by estrogen receptor status and its clinical significance.
Yen MC, Kan JY, Hsieh CJ, Kuo PL, Hou MF, Hsu YL
Oncology reports37(6)3253-3260(2017 Jun)

Low acyl-CoA synthetase 5 expression in colorectal carcinomas is prognostic for early tumour recurrence.
Hartmann F, Sparla D, Tute E, Tamm M, Schneider U, Jeon MK, Kasperk R, Gassler N, Kaemmerer E
Pathology, research and practice213(3)261-266(2017 Mar)

Acyl-CoA Synthetase 5 Promotes the Growth and Invasion of Colorectal Cancer Cells.
Ding S, Tang S, Wang M, Wu D, Guo H
Canadian journal of gastroenterology & hepatology20177615736(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

colorectal adenocarcinomas with low (n=41; group 1) or high (n=31; group 2) ACSL5 levels were identified. In a one-year follow-up, tumour recurrence was significantly increased in group 1.
Title: Low acyl-CoA synthetase 5 expression in colorectal carcinomas is prognostic for early tumour recurrence.

Functional variant (rs2256368:A>G) affecting ACSL5 exon 20 skipping was identified as a causal factor linked to the migraine-associated rs12355831:A>G, suggesting that the activation of long-chain fatty acids by the spliced ACSL5-Delta20 molecules is involved in migraine pathology.
Title: A splice variant in the ACSL5 gene relates migraine with fatty acid activation in mitochondria.

ACSL5 mediates antiproliferative activities via Wnt2B palmitoylation with diminished Wnt activity. The molecular pathway is probably relevant for intestinal homeostasis, overwhelmed by other pathways in carcinogenesis.
Title: Modulating effects of acyl-CoA synthetase 5-derived mitochondrial Wnt2B palmitoylation on intestinal Wnt activity.

Uncoupling of ACSL5 and mitochondrial mortalin by mutated TP53 could be important in colorectal carcinogenesis.
Title: TP53 status regulates ACSL5-induced expression of mitochondrial mortalin in enterocytes and colorectal adenocarcinomas.

Down-regulation of ACSL5 is associated with colorectal cancer.
Title: Differential expression and prognostic role of selected genes in colorectal cancer patients.

The following ACSL5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACSL5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02292	NM_016234.3 Latest version!	Homo sapiens acyl-CoA synthetase long chain family member 5 (ACSL5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu02964	NM_203379.2 Latest version!	Homo sapiens acyl-CoA synthetase long chain family member 5 (ACSL5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu02964	NM_203380.1 Latest version!	Homo sapiens acyl-CoA synthetase long chain family member 5 (ACSL5), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu91462	XM_017016324.2 Latest version!	Homo sapiens acyl-CoA synthetase long chain family member 5 (ACSL5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu91462	XM_017016324.1	Homo sapiens acyl-CoA synthetase long-chain family member 5 (ACSL5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu02964	NM_203379.1	Homo sapiens acyl-CoA synthetase long chain family member 5 (ACSL5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACSL5 ( NM_016234.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_057318.2 Homo sapiens acyl-CoA synthetase long chain family member 5 (ACSL5), transcript variant 1, mRNA.

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CloneID	OHu02292
Clone ID Related Accession (Same CDS sequence)	NM_016234.3
Accession Version	NM_016234.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Homo sapiens(human)
Product	long-chain-fatty-acid--CoA ligase 5 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL708480.1, AY358520.1, AK024932.1 and AK000339.1. On Feb 25, 2004 this sequence version replaced NM_016234.2. Transcript Variant: This variant (1) encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK024932.1, SRR1803615.70193.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACGCTC TGAAGCCACC CTGTCTCTGG AGGAACCACG AGCGAGGGAA GAAGGACAGG 
GACTCGTGTG GCAGGAAGAA CTCAGAGCCG GGAAGCCCCC ATTCACTAGA AGCACTGAGA 
GATGCGGCCC CCTCGCAGGG TCTGAATTTC CTGCTGCTGT TCACAAAGAT GCTTTTTATC 
TTTAACTTTT TGTTTTCCCC ACTTCCGACC CCGGCGTTGA TCTGCATCCT GACATTTGGA 
GCTGCCATCT TCTTGTGGCT GATCACCAGA CCTCAACCCG TCTTACCTCT TCTTGACCTG 
AACAATCAGT CTGTGGGAAT TGAGGGAGGA GCACGGAAGG GGGTTTCCCA GAAGAACAAT 
GACCTAACAA GTTGCTGCTT CTCAGATGCC AAGACTATGT ATGAGGTTTT CCAAAGAGGA 
CTCGCTGTGT CTGACAATGG GCCCTGCTTG GGATATAGAA AACCAAACCA GCCCTACAGA 
TGGCTATCTT ACAAACAGGT GTCTGATAGA GCAGAGTACC TGGGTTCCTG TCTCTTGCAT 
AAAGGTTATA AATCATCACC AGACCAGTTT GTCGGCATCT TTGCTCAGAA TAGGCCAGAG 
TGGATCATCT CCGAATTGGC TTGTTACACG TACTCTATGG TAGCTGTACC TCTGTATGAC 
ACCTTGGGAC CAGAAGCCAT CGTACATATT GTCAACAAGG CTGATATCGC CATGGTGATC 
TGTGACACAC CCCAAAAGGC ATTGGTGCTG ATAGGGAATG TAGAGAAAGG CTTCACCCCG 
AGCCTGAAGG TGATCATCCT TATGGACCCC TTTGATGATG ACCTGAAGCA AAGAGGGGAG 
AAGAGTGGAA TTGAGATCTT ATCCCTATAT GATGCTGAGA ACCTAGGCAA AGAGCACTTC 
AGAAAACCTG TGCCTCCTAG CCCAGAAGAC CTGAGCGTCA TCTGCTTCAC CAGTGGGACC 
ACAGGTGACC CCAAAGGAGC CATGATAACC CATCAAAATA TTGTTTCAAA TGCTGCTGCC 
TTTCTCAAAT GTGTGGAGCA TGCTTATGAG CCCACTCCTG ATGATGTGGC CATATCCTAC 
CTCCCTCTGG CTCATATGTT TGAGAGGATT GTACAGGCTG TTGTGTACAG CTGTGGAGCC 
AGAGTTGGAT TCTTCCAAGG GGATATTCGG TTGCTGGCTG ACGACATGAA GACTTTGAAG 
CCCACATTGT TTCCCGCGGT GCCTCGACTC CTTAACAGGA TCTACGATAA GGTACAAAAT 
GAGGCCAAGA CACCCTTGAA GAAGTTCTTG TTGAAGCTGG CTGTTTCCAG TAAATTCAAA 
GAGCTTCAAA AGGGTATCAT CAGGCATGAT AGTTTCTGGG ACAAGCTCAT CTTTGCAAAG 
ATCCAGGACA GCCTGGGCGG AAGGGTTCGT GTAATTGTCA CTGGAGCTGC CCCCATGTCC 
ACTTCAGTCA TGACATTCTT CCGGGCAGCA ATGGGATGTC AGGTGTATGA AGCTTATGGT 
CAAACAGAAT GCACAGGTGG CTGTACATTT ACATTACCTG GGGACTGGAC ATCAGGTCAC 
GTTGGGGTGC CCCTGGCTTG CAATTACGTG AAGCTGGAAG ATGTGGCTGA CATGAACTAC 
TTTACAGTGA ATAATGAAGG AGAGGTCTGC ATCAAGGGTA CAAACGTGTT CAAAGGATAC 
CTGAAGGACC CTGAGAAGAC ACAGGAAGCC CTGGACAGTG ATGGCTGGCT TCACACAGGA 
GACATTGGTC GCTGGCTCCC GAATGGAACT CTGAAGATCA TCGACCGTAA AAAGAACATT 
TTCAAGCTGG CCCAAGGAGA ATACATTGCA CCAGAGAAGA TAGAAAATAT CTACAACAGG 
AGTCAACCAG TGTTACAAAT TTTTGTACAC GGGGAGAGCT TACGGTCATC CTTAGTAGGA 
GTGGTGGTTC CTGACACAGA TGTACTTCCC TCATTTGCAG CCAAGCTTGG GGTGAAGGGC 
TCCTTTGAGG AACTGTGCCA AAACCAAGTT GTAAGGGAAG CCATTTTAGA AGACTTGCAG 
AAAATTGGGA AAGAAAGTGG CCTTAAAACT TTTGAACAGG TCAAAGCCAT TTTTCTTCAT 
CCAGAGCCAT TTTCCATTGA AAATGGGCTC TTGACACCAA CATTGAAAGC AAAGCGAGGA 
GAGCTTTCCA AATACTTTCG GACCCAAATT GACAGCCTGT ATGAGCACAT CCAGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057318.2
CDS	113..2332
Translation	MDALKPPCLWRNHERGKKDRDSCGRKNSEPGSPHSLEALRDAAPSQGLNFLLLFTKMLFIFNFLFSPLPTPALICILTFGAAIFLWLITRPQPVLPLLDLNNQSVGIEGGARKGVSQKNNDLTSCCFSDAKTMYEVFQRGLAVSDNGPCLGYRKPNQPYRWLSYKQVSDRAEYLGSCLLHKGYKSSPDQFVGIFAQNRPEWIISELACYTYSMVAVPLYDTLGPEAIVHIVNKADIAMVICDTPQKALVLIGNVEKGFTPSLKVIILMDPFDDDLKQRGEKSGIEILSLYDAENLGKEHFRKPVPPSPEDLSVICFTSGTTGDPKGAMITHQNIVSNAAAFLKCVEHAYEPTPDDVAISYLPLAHMFERIVQAVVYSCGARVGFFQGDIRLLADDMKTLKPTLFPAVPRLLNRIYDKVQNEAKTPLKKFLLKLAVSSKFKELQKGIIRHDSFWDKLIFAKIQDSLGGRVRVIVTGAAPMSTSVMTFFRAAMGCQVYEAYGQTECTGGCTFTLPGDWTSGHVGVPLACNYVKLEDVADMNYFTVNNEGEVCIKGTNVFKGYLKDPEKTQEALDSDGWLHTGDIGRWLPNGTLKIIDRKKNIFKLAQGEYIAPEKIENIYNRSQPVLQIFVHGESLRSSLVGVVVPDTDVLPSFAAKLGVKGSFEELCQNQVVREAILEDLQKIGKESGLKTFEQVKAIFLHPEPFSIENGLLTPTLKAKRGELSKYFRTQIDSLYEHIQD

Target ORF information:

RefSeq Version	NM_016234.3
Organism	Homo sapiens(human)
Definition	Homo sapiens acyl-CoA synthetase long chain family member 5 (ACSL5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016234.3

ORF Insert Sequence:

ATGGACGCTC TGAAGCCACC CTGTCTCTGG AGGAACCACG AGCGAGGGAA GAAGGACAGG 
GACTCGTGTG GCAGGAAGAA CTCAGAGCCG GGAAGCCCCC ATTCACTAGA AGCACTGAGA 
GATGCGGCCC CCTCGCAGGG TCTGAATTTC CTGCTGCTGT TCACAAAGAT GCTTTTTATC 
TTTAACTTTT TGTTTTCCCC ACTTCCGACC CCGGCGTTGA TCTGCATCCT GACATTTGGA 
GCTGCCATCT TCTTGTGGCT GATCACCAGA CCTCAACCCG TCTTACCTCT TCTTGACCTG 
AACAATCAGT CTGTGGGAAT TGAGGGAGGA GCACGGAAGG GGGTTTCCCA GAAGAACAAT 
GACCTAACAA GTTGCTGCTT CTCAGATGCC AAGACTATGT ATGAGGTTTT CCAAAGAGGA 
CTCGCTGTGT CTGACAATGG GCCCTGCTTG GGATATAGAA AACCAAACCA GCCCTACAGA 
TGGCTATCTT ACAAACAGGT GTCTGATAGA GCAGAGTACC TGGGTTCCTG TCTCTTGCAT 
AAAGGTTATA AATCATCACC AGACCAGTTT GTCGGCATCT TTGCTCAGAA TAGGCCAGAG 
TGGATCATCT CCGAATTGGC TTGTTACACG TACTCTATGG TAGCTGTACC TCTGTATGAC 
ACCTTGGGAC CAGAAGCCAT CGTACATATT GTCAACAAGG CTGATATCGC CATGGTGATC 
TGTGACACAC CCCAAAAGGC ATTGGTGCTG ATAGGGAATG TAGAGAAAGG CTTCACCCCG 
AGCCTGAAGG TGATCATCCT TATGGACCCC TTTGATGATG ACCTGAAGCA AAGAGGGGAG 
AAGAGTGGAA TTGAGATCTT ATCCCTATAT GATGCTGAGA ACCTAGGCAA AGAGCACTTC 
AGAAAACCTG TGCCTCCTAG CCCAGAAGAC CTGAGCGTCA TCTGCTTCAC CAGTGGGACC 
ACAGGTGACC CCAAAGGAGC CATGATAACC CATCAAAATA TTGTTTCAAA TGCTGCTGCC 
TTTCTCAAAT GTGTGGAGCA TGCTTATGAG CCCACTCCTG ATGATGTGGC CATATCCTAC 
CTCCCTCTGG CTCATATGTT TGAGAGGATT GTACAGGCTG TTGTGTACAG CTGTGGAGCC 
AGAGTTGGAT TCTTCCAAGG GGATATTCGG TTGCTGGCTG ACGACATGAA GACTTTGAAG 
CCCACATTGT TTCCCGCGGT GCCTCGACTC CTTAACAGGA TCTACGATAA GGTACAAAAT 
GAGGCCAAGA CACCCTTGAA GAAGTTCTTG TTGAAGCTGG CTGTTTCCAG TAAATTCAAA 
GAGCTTCAAA AGGGTATCAT CAGGCATGAT AGTTTCTGGG ACAAGCTCAT CTTTGCAAAG 
ATCCAGGACA GCCTGGGCGG AAGGGTTCGT GTAATTGTCA CTGGAGCTGC CCCCATGTCC 
ACTTCAGTCA TGACATTCTT CCGGGCAGCA ATGGGATGTC AGGTGTATGA AGCTTATGGT 
CAAACAGAAT GCACAGGTGG CTGTACATTT ACATTACCTG GGGACTGGAC ATCAGGTCAC 
GTTGGGGTGC CCCTGGCTTG CAATTACGTG AAGCTGGAAG ATGTGGCTGA CATGAACTAC 
TTTACAGTGA ATAATGAAGG AGAGGTCTGC ATCAAGGGTA CAAACGTGTT CAAAGGATAC 
CTGAAGGACC CTGAGAAGAC ACAGGAAGCC CTGGACAGTG ATGGCTGGCT TCACACAGGA 
GACATTGGTC GCTGGCTCCC GAATGGAACT CTGAAGATCA TCGACCGTAA AAAGAACATT 
TTCAAGCTGG CCCAAGGAGA ATACATTGCA CCAGAGAAGA TAGAAAATAT CTACAACAGG 
AGTCAACCAG TGTTACAAAT TTTTGTACAC GGGGAGAGCT TACGGTCATC CTTAGTAGGA 
GTGGTGGTTC CTGACACAGA TGTACTTCCC TCATTTGCAG CCAAGCTTGG GGTGAAGGGC 
TCCTTTGAGG AACTGTGCCA AAACCAAGTT GTAAGGGAAG CCATTTTAGA AGACTTGCAG 
AAAATTGGGA AAGAAAGTGG CCTTAAAACT TTTGAACAGG TCAAAGCCAT TTTTCTTCAT 
CCAGAGCCAT TTTCCATTGA AAATGGGCTC TTGACACCAA CATTGAAAGC AAAGCGAGGA 
GAGCTTTCCA AATACTTTCG GACCCAAATT GACAGCCTGT ATGAGCACAT CCAGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACSL5 ( NM_203379.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_976313.1 Homo sapiens acyl-CoA synthetase long chain family member 5 (ACSL5), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu02964
Clone ID Related Accession (Same CDS sequence)	NM_203379.1 , NM_203380.1 , NM_203379.2
Accession Version	NM_203379.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Homo sapiens(human)
Product	long-chain-fatty-acid--CoA ligase 5 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK000339.1, CF595490.1, AY358520.1 and AK024932.1. Transcript Variant: This variant (2) differs in the 5' UTR and coding region compared to variant 1. This results in translation initiation from a downstream ATG and an isoform (b) that has a shorter N-terminus compared to isoform a. Both variants 2 and 3 encode isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK000339.1, SRR1660803.91901.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTTTTTA TCTTTAACTT TTTGTTTTCC CCACTTCCGA CCCCGGCGTT GATCTGCATC 
CTGACATTTG GAGCTGCCAT CTTCTTGTGG CTGATCACCA GACCTCAACC CGTCTTACCT 
CTTCTTGACC TGAACAATCA GTCTGTGGGA ATTGAGGGAG GAGCACGGAA GGGGGTTTCC 
CAGAAGAACA ATGACCTAAC AAGTTGCTGC TTCTCAGATG CCAAGACTAT GTATGAGGTT 
TTCCAAAGAG GACTCGCTGT GTCTGACAAT GGGCCCTGCT TGGGATATAG AAAACCAAAC 
CAGCCCTACA GATGGCTATC TTACAAACAG GTGTCTGATA GAGCAGAGTA CCTGGGTTCC 
TGTCTCTTGC ATAAAGGTTA TAAATCATCA CCAGACCAGT TTGTCGGCAT CTTTGCTCAG 
AATAGGCCAG AGTGGATCAT CTCCGAATTG GCTTGTTACA CGTACTCTAT GGTAGCTGTA 
CCTCTGTATG ACACCTTGGG ACCAGAAGCC ATCGTACATA TTGTCAACAA GGCTGATATC 
GCCATGGTGA TCTGTGACAC ACCCCAAAAG GCATTGGTGC TGATAGGGAA TGTAGAGAAA 
GGCTTCACCC CGAGCCTGAA GGTGATCATC CTTATGGACC CCTTTGATGA TGACCTGAAG 
CAAAGAGGGG AGAAGAGTGG AATTGAGATC TTATCCCTAT ATGATGCTGA GAACCTAGGC 
AAAGAGCACT TCAGAAAACC TGTGCCTCCT AGCCCAGAAG ACCTGAGCGT CATCTGCTTC 
ACCAGTGGGA CCACAGGTGA CCCCAAAGGA GCCATGATAA CCCATCAAAA TATTGTTTCA 
AATGCTGCTG CCTTTCTCAA ATGTGTGGAG CATGCTTATG AGCCCACTCC TGATGATGTG 
GCCATATCCT ACCTCCCTCT GGCTCATATG TTTGAGAGGA TTGTACAGGC TGTTGTGTAC 
AGCTGTGGAG CCAGAGTTGG ATTCTTCCAA GGGGATATTC GGTTGCTGGC TGACGACATG 
AAGACTTTGA AGCCCACATT GTTTCCCGCG GTGCCTCGAC TCCTTAACAG GATCTACGAT 
AAGGTACAAA ATGAGGCCAA GACACCCTTG AAGAAGTTCT TGTTGAAGCT GGCTGTTTCC 
AGTAAATTCA AAGAGCTTCA AAAGGGTATC ATCAGGCATG ATAGTTTCTG GGACAAGCTC 
ATCTTTGCAA AGATCCAGGA CAGCCTGGGC GGAAGGGTTC GTGTAATTGT CACTGGAGCT 
GCCCCCATGT CCACTTCAGT CATGACATTC TTCCGGGCAG CAATGGGATG TCAGGTGTAT 
GAAGCTTATG GTCAAACAGA ATGCACAGGT GGCTGTACAT TTACATTACC TGGGGACTGG 
ACATCAGGTC ACGTTGGGGT GCCCCTGGCT TGCAATTACG TGAAGCTGGA AGATGTGGCT 
GACATGAACT ACTTTACAGT GAATAATGAA GGAGAGGTCT GCATCAAGGG TACAAACGTG 
TTCAAAGGAT ACCTGAAGGA CCCTGAGAAG ACACAGGAAG CCCTGGACAG TGATGGCTGG 
CTTCACACAG GAGACATTGG TCGCTGGCTC CCGAATGGAA CTCTGAAGAT CATCGACCGT 
AAAAAGAACA TTTTCAAGCT GGCCCAAGGA GAATACATTG CACCAGAGAA GATAGAAAAT 
ATCTACAACA GGAGTCAACC AGTGTTACAA ATTTTTGTAC ACGGGGAGAG CTTACGGTCA 
TCCTTAGTAG GAGTGGTGGT TCCTGACACA GATGTACTTC CCTCATTTGC AGCCAAGCTT 
GGGGTGAAGG GCTCCTTTGA GGAACTGTGC CAAAACCAAG TTGTAAGGGA AGCCATTTTA 
GAAGACTTGC AGAAAATTGG GAAAGAAAGT GGCCTTAAAA CTTTTGAACA GGTCAAAGCC 
ATTTTTCTTC ATCCAGAGCC ATTTTCCATT GAAAATGGGC TCTTGACACC AACATTGAAA 
GCAAAGCGAG GAGAGCTTTC CAAATACTTT CGGACCCAAA TTGACAGCCT GTATGAGCAC 
ATCCAGGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_976313.1
CDS	142..2193
Translation	MLFIFNFLFSPLPTPALICILTFGAAIFLWLITRPQPVLPLLDLNNQSVGIEGGARKGVSQKNNDLTSCCFSDAKTMYEVFQRGLAVSDNGPCLGYRKPNQPYRWLSYKQVSDRAEYLGSCLLHKGYKSSPDQFVGIFAQNRPEWIISELACYTYSMVAVPLYDTLGPEAIVHIVNKADIAMVICDTPQKALVLIGNVEKGFTPSLKVIILMDPFDDDLKQRGEKSGIEILSLYDAENLGKEHFRKPVPPSPEDLSVICFTSGTTGDPKGAMITHQNIVSNAAAFLKCVEHAYEPTPDDVAISYLPLAHMFERIVQAVVYSCGARVGFFQGDIRLLADDMKTLKPTLFPAVPRLLNRIYDKVQNEAKTPLKKFLLKLAVSSKFKELQKGIIRHDSFWDKLIFAKIQDSLGGRVRVIVTGAAPMSTSVMTFFRAAMGCQVYEAYGQTECTGGCTFTLPGDWTSGHVGVPLACNYVKLEDVADMNYFTVNNEGEVCIKGTNVFKGYLKDPEKTQEALDSDGWLHTGDIGRWLPNGTLKIIDRKKNIFKLAQGEYIAPEKIENIYNRSQPVLQIFVHGESLRSSLVGVVVPDTDVLPSFAAKLGVKGSFEELCQNQVVREAILEDLQKIGKESGLKTFEQVKAIFLHPEPFSIENGLLTPTLKAKRGELSKYFRTQIDSLYEHIQD

Target ORF information:

RefSeq Version	NM_203379.1
Organism	Homo sapiens(human)
Definition	Homo sapiens acyl-CoA synthetase long chain family member 5 (ACSL5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_203379.1

ORF Insert Sequence:

ATGCTTTTTA TCTTTAACTT TTTGTTTTCC CCACTTCCGA CCCCGGCGTT GATCTGCATC 
CTGACATTTG GAGCTGCCAT CTTCTTGTGG CTGATCACCA GACCTCAACC CGTCTTACCT 
CTTCTTGACC TGAACAATCA GTCTGTGGGA ATTGAGGGAG GAGCACGGAA GGGGGTTTCC 
CAGAAGAACA ATGACCTAAC AAGTTGCTGC TTCTCAGATG CCAAGACTAT GTATGAGGTT 
TTCCAAAGAG GACTCGCTGT GTCTGACAAT GGGCCCTGCT TGGGATATAG AAAACCAAAC 
CAGCCCTACA GATGGCTATC TTACAAACAG GTGTCTGATA GAGCAGAGTA CCTGGGTTCC 
TGTCTCTTGC ATAAAGGTTA TAAATCATCA CCAGACCAGT TTGTCGGCAT CTTTGCTCAG 
AATAGGCCAG AGTGGATCAT CTCCGAATTG GCTTGTTACA CGTACTCTAT GGTAGCTGTA 
CCTCTGTATG ACACCTTGGG ACCAGAAGCC ATCGTACATA TTGTCAACAA GGCTGATATC 
GCCATGGTGA TCTGTGACAC ACCCCAAAAG GCATTGGTGC TGATAGGGAA TGTAGAGAAA 
GGCTTCACCC CGAGCCTGAA GGTGATCATC CTTATGGACC CCTTTGATGA TGACCTGAAG 
CAAAGAGGGG AGAAGAGTGG AATTGAGATC TTATCCCTAT ATGATGCTGA GAACCTAGGC 
AAAGAGCACT TCAGAAAACC TGTGCCTCCT AGCCCAGAAG ACCTGAGCGT CATCTGCTTC 
ACCAGTGGGA CCACAGGTGA CCCCAAAGGA GCCATGATAA CCCATCAAAA TATTGTTTCA 
AATGCTGCTG CCTTTCTCAA ATGTGTGGAG CATGCTTATG AGCCCACTCC TGATGATGTG 
GCCATATCCT ACCTCCCTCT GGCTCATATG TTTGAGAGGA TTGTACAGGC TGTTGTGTAC 
AGCTGTGGAG CCAGAGTTGG ATTCTTCCAA GGGGATATTC GGTTGCTGGC TGACGACATG 
AAGACTTTGA AGCCCACATT GTTTCCCGCG GTGCCTCGAC TCCTTAACAG GATCTACGAT 
AAGGTACAAA ATGAGGCCAA GACACCCTTG AAGAAGTTCT TGTTGAAGCT GGCTGTTTCC 
AGTAAATTCA AAGAGCTTCA AAAGGGTATC ATCAGGCATG ATAGTTTCTG GGACAAGCTC 
ATCTTTGCAA AGATCCAGGA CAGCCTGGGC GGAAGGGTTC GTGTAATTGT CACTGGAGCT 
GCCCCCATGT CCACTTCAGT CATGACATTC TTCCGGGCAG CAATGGGATG TCAGGTGTAT 
GAAGCTTATG GTCAAACAGA ATGCACAGGT GGCTGTACAT TTACATTACC TGGGGACTGG 
ACATCAGGTC ACGTTGGGGT GCCCCTGGCT TGCAATTACG TGAAGCTGGA AGATGTGGCT 
GACATGAACT ACTTTACAGT GAATAATGAA GGAGAGGTCT GCATCAAGGG TACAAACGTG 
TTCAAAGGAT ACCTGAAGGA CCCTGAGAAG ACACAGGAAG CCCTGGACAG TGATGGCTGG 
CTTCACACAG GAGACATTGG TCGCTGGCTC CCGAATGGAA CTCTGAAGAT CATCGACCGT 
AAAAAGAACA TTTTCAAGCT GGCCCAAGGA GAATACATTG CACCAGAGAA GATAGAAAAT 
ATCTACAACA GGAGTCAACC AGTGTTACAA ATTTTTGTAC ACGGGGAGAG CTTACGGTCA 
TCCTTAGTAG GAGTGGTGGT TCCTGACACA GATGTACTTC CCTCATTTGC AGCCAAGCTT 
GGGGTGAAGG GCTCCTTTGA GGAACTGTGC CAAAACCAAG TTGTAAGGGA AGCCATTTTA 
GAAGACTTGC AGAAAATTGG GAAAGAAAGT GGCCTTAAAA CTTTTGAACA GGTCAAAGCC 
ATTTTTCTTC ATCCAGAGCC ATTTTCCATT GAAAATGGGC TCTTGACACC AACATTGAAA 
GCAAAGCGAG GAGAGCTTTC CAAATACTTT CGGACCCAAA TTGACAGCCT GTATGAGCAC 
ATCCAGGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACSL5 ( NM_203380.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_976314.1 Homo sapiens acyl-CoA synthetase long chain family member 5 (ACSL5), transcript variant 3, mRNA.

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CloneID	OHu02964
Clone ID Related Accession (Same CDS sequence)	NM_203379.1 , NM_203380.1 , NM_203379.2
Accession Version	NM_203380.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Homo sapiens(human)
Product	long-chain-fatty-acid--CoA ligase 5 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD689921.1, AL708480.1, AY358520.1, AK024932.1 and AK000339.1. Transcript Variant: This variant (3) differs in the 5' UTR and coding region compared to variant 1. This results in translation initiation from a downstream ATG and an isoform (b) that has a shorter N-terminus compared to isoform a. Both variants 2 and 3 encode isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.279490.1, SRR1660805.116816.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTTTTTA TCTTTAACTT TTTGTTTTCC CCACTTCCGA CCCCGGCGTT GATCTGCATC 
CTGACATTTG GAGCTGCCAT CTTCTTGTGG CTGATCACCA GACCTCAACC CGTCTTACCT 
CTTCTTGACC TGAACAATCA GTCTGTGGGA ATTGAGGGAG GAGCACGGAA GGGGGTTTCC 
CAGAAGAACA ATGACCTAAC AAGTTGCTGC TTCTCAGATG CCAAGACTAT GTATGAGGTT 
TTCCAAAGAG GACTCGCTGT GTCTGACAAT GGGCCCTGCT TGGGATATAG AAAACCAAAC 
CAGCCCTACA GATGGCTATC TTACAAACAG GTGTCTGATA GAGCAGAGTA CCTGGGTTCC 
TGTCTCTTGC ATAAAGGTTA TAAATCATCA CCAGACCAGT TTGTCGGCAT CTTTGCTCAG 
AATAGGCCAG AGTGGATCAT CTCCGAATTG GCTTGTTACA CGTACTCTAT GGTAGCTGTA 
CCTCTGTATG ACACCTTGGG ACCAGAAGCC ATCGTACATA TTGTCAACAA GGCTGATATC 
GCCATGGTGA TCTGTGACAC ACCCCAAAAG GCATTGGTGC TGATAGGGAA TGTAGAGAAA 
GGCTTCACCC CGAGCCTGAA GGTGATCATC CTTATGGACC CCTTTGATGA TGACCTGAAG 
CAAAGAGGGG AGAAGAGTGG AATTGAGATC TTATCCCTAT ATGATGCTGA GAACCTAGGC 
AAAGAGCACT TCAGAAAACC TGTGCCTCCT AGCCCAGAAG ACCTGAGCGT CATCTGCTTC 
ACCAGTGGGA CCACAGGTGA CCCCAAAGGA GCCATGATAA CCCATCAAAA TATTGTTTCA 
AATGCTGCTG CCTTTCTCAA ATGTGTGGAG CATGCTTATG AGCCCACTCC TGATGATGTG 
GCCATATCCT ACCTCCCTCT GGCTCATATG TTTGAGAGGA TTGTACAGGC TGTTGTGTAC 
AGCTGTGGAG CCAGAGTTGG ATTCTTCCAA GGGGATATTC GGTTGCTGGC TGACGACATG 
AAGACTTTGA AGCCCACATT GTTTCCCGCG GTGCCTCGAC TCCTTAACAG GATCTACGAT 
AAGGTACAAA ATGAGGCCAA GACACCCTTG AAGAAGTTCT TGTTGAAGCT GGCTGTTTCC 
AGTAAATTCA AAGAGCTTCA AAAGGGTATC ATCAGGCATG ATAGTTTCTG GGACAAGCTC 
ATCTTTGCAA AGATCCAGGA CAGCCTGGGC GGAAGGGTTC GTGTAATTGT CACTGGAGCT 
GCCCCCATGT CCACTTCAGT CATGACATTC TTCCGGGCAG CAATGGGATG TCAGGTGTAT 
GAAGCTTATG GTCAAACAGA ATGCACAGGT GGCTGTACAT TTACATTACC TGGGGACTGG 
ACATCAGGTC ACGTTGGGGT GCCCCTGGCT TGCAATTACG TGAAGCTGGA AGATGTGGCT 
GACATGAACT ACTTTACAGT GAATAATGAA GGAGAGGTCT GCATCAAGGG TACAAACGTG 
TTCAAAGGAT ACCTGAAGGA CCCTGAGAAG ACACAGGAAG CCCTGGACAG TGATGGCTGG 
CTTCACACAG GAGACATTGG TCGCTGGCTC CCGAATGGAA CTCTGAAGAT CATCGACCGT 
AAAAAGAACA TTTTCAAGCT GGCCCAAGGA GAATACATTG CACCAGAGAA GATAGAAAAT 
ATCTACAACA GGAGTCAACC AGTGTTACAA ATTTTTGTAC ACGGGGAGAG CTTACGGTCA 
TCCTTAGTAG GAGTGGTGGT TCCTGACACA GATGTACTTC CCTCATTTGC AGCCAAGCTT 
GGGGTGAAGG GCTCCTTTGA GGAACTGTGC CAAAACCAAG TTGTAAGGGA AGCCATTTTA 
GAAGACTTGC AGAAAATTGG GAAAGAAAGT GGCCTTAAAA CTTTTGAACA GGTCAAAGCC 
ATTTTTCTTC ATCCAGAGCC ATTTTCCATT GAAAATGGGC TCTTGACACC AACATTGAAA 
GCAAAGCGAG GAGAGCTTTC CAAATACTTT CGGACCCAAA TTGACAGCCT GTATGAGCAC 
ATCCAGGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_976314.1
CDS	308..2359
Translation	MLFIFNFLFSPLPTPALICILTFGAAIFLWLITRPQPVLPLLDLNNQSVGIEGGARKGVSQKNNDLTSCCFSDAKTMYEVFQRGLAVSDNGPCLGYRKPNQPYRWLSYKQVSDRAEYLGSCLLHKGYKSSPDQFVGIFAQNRPEWIISELACYTYSMVAVPLYDTLGPEAIVHIVNKADIAMVICDTPQKALVLIGNVEKGFTPSLKVIILMDPFDDDLKQRGEKSGIEILSLYDAENLGKEHFRKPVPPSPEDLSVICFTSGTTGDPKGAMITHQNIVSNAAAFLKCVEHAYEPTPDDVAISYLPLAHMFERIVQAVVYSCGARVGFFQGDIRLLADDMKTLKPTLFPAVPRLLNRIYDKVQNEAKTPLKKFLLKLAVSSKFKELQKGIIRHDSFWDKLIFAKIQDSLGGRVRVIVTGAAPMSTSVMTFFRAAMGCQVYEAYGQTECTGGCTFTLPGDWTSGHVGVPLACNYVKLEDVADMNYFTVNNEGEVCIKGTNVFKGYLKDPEKTQEALDSDGWLHTGDIGRWLPNGTLKIIDRKKNIFKLAQGEYIAPEKIENIYNRSQPVLQIFVHGESLRSSLVGVVVPDTDVLPSFAAKLGVKGSFEELCQNQVVREAILEDLQKIGKESGLKTFEQVKAIFLHPEPFSIENGLLTPTLKAKRGELSKYFRTQIDSLYEHIQD

Target ORF information:

RefSeq Version	NM_203380.1
Organism	Homo sapiens(human)
Definition	Homo sapiens acyl-CoA synthetase long chain family member 5 (ACSL5), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_203380.1

ORF Insert Sequence:

ATGCTTTTTA TCTTTAACTT TTTGTTTTCC CCACTTCCGA CCCCGGCGTT GATCTGCATC 
CTGACATTTG GAGCTGCCAT CTTCTTGTGG CTGATCACCA GACCTCAACC CGTCTTACCT 
CTTCTTGACC TGAACAATCA GTCTGTGGGA ATTGAGGGAG GAGCACGGAA GGGGGTTTCC 
CAGAAGAACA ATGACCTAAC AAGTTGCTGC TTCTCAGATG CCAAGACTAT GTATGAGGTT 
TTCCAAAGAG GACTCGCTGT GTCTGACAAT GGGCCCTGCT TGGGATATAG AAAACCAAAC 
CAGCCCTACA GATGGCTATC TTACAAACAG GTGTCTGATA GAGCAGAGTA CCTGGGTTCC 
TGTCTCTTGC ATAAAGGTTA TAAATCATCA CCAGACCAGT TTGTCGGCAT CTTTGCTCAG 
AATAGGCCAG AGTGGATCAT CTCCGAATTG GCTTGTTACA CGTACTCTAT GGTAGCTGTA 
CCTCTGTATG ACACCTTGGG ACCAGAAGCC ATCGTACATA TTGTCAACAA GGCTGATATC 
GCCATGGTGA TCTGTGACAC ACCCCAAAAG GCATTGGTGC TGATAGGGAA TGTAGAGAAA 
GGCTTCACCC CGAGCCTGAA GGTGATCATC CTTATGGACC CCTTTGATGA TGACCTGAAG 
CAAAGAGGGG AGAAGAGTGG AATTGAGATC TTATCCCTAT ATGATGCTGA GAACCTAGGC 
AAAGAGCACT TCAGAAAACC TGTGCCTCCT AGCCCAGAAG ACCTGAGCGT CATCTGCTTC 
ACCAGTGGGA CCACAGGTGA CCCCAAAGGA GCCATGATAA CCCATCAAAA TATTGTTTCA 
AATGCTGCTG CCTTTCTCAA ATGTGTGGAG CATGCTTATG AGCCCACTCC TGATGATGTG 
GCCATATCCT ACCTCCCTCT GGCTCATATG TTTGAGAGGA TTGTACAGGC TGTTGTGTAC 
AGCTGTGGAG CCAGAGTTGG ATTCTTCCAA GGGGATATTC GGTTGCTGGC TGACGACATG 
AAGACTTTGA AGCCCACATT GTTTCCCGCG GTGCCTCGAC TCCTTAACAG GATCTACGAT 
AAGGTACAAA ATGAGGCCAA GACACCCTTG AAGAAGTTCT TGTTGAAGCT GGCTGTTTCC 
AGTAAATTCA AAGAGCTTCA AAAGGGTATC ATCAGGCATG ATAGTTTCTG GGACAAGCTC 
ATCTTTGCAA AGATCCAGGA CAGCCTGGGC GGAAGGGTTC GTGTAATTGT CACTGGAGCT 
GCCCCCATGT CCACTTCAGT CATGACATTC TTCCGGGCAG CAATGGGATG TCAGGTGTAT 
GAAGCTTATG GTCAAACAGA ATGCACAGGT GGCTGTACAT TTACATTACC TGGGGACTGG 
ACATCAGGTC ACGTTGGGGT GCCCCTGGCT TGCAATTACG TGAAGCTGGA AGATGTGGCT 
GACATGAACT ACTTTACAGT GAATAATGAA GGAGAGGTCT GCATCAAGGG TACAAACGTG 
TTCAAAGGAT ACCTGAAGGA CCCTGAGAAG ACACAGGAAG CCCTGGACAG TGATGGCTGG 
CTTCACACAG GAGACATTGG TCGCTGGCTC CCGAATGGAA CTCTGAAGAT CATCGACCGT 
AAAAAGAACA TTTTCAAGCT GGCCCAAGGA GAATACATTG CACCAGAGAA GATAGAAAAT 
ATCTACAACA GGAGTCAACC AGTGTTACAA ATTTTTGTAC ACGGGGAGAG CTTACGGTCA 
TCCTTAGTAG GAGTGGTGGT TCCTGACACA GATGTACTTC CCTCATTTGC AGCCAAGCTT 
GGGGTGAAGG GCTCCTTTGA GGAACTGTGC CAAAACCAAG TTGTAAGGGA AGCCATTTTA 
GAAGACTTGC AGAAAATTGG GAAAGAAAGT GGCCTTAAAA CTTTTGAACA GGTCAAAGCC 
ATTTTTCTTC ATCCAGAGCC ATTTTCCATT GAAAATGGGC TCTTGACACC AACATTGAAA 
GCAAAGCGAG GAGAGCTTTC CAAATACTTT CGGACCCAAA TTGACAGCCT GTATGAGCAC 
ATCCAGGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACSL5 ( NM_203379.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_976313.1 Homo sapiens acyl-CoA synthetase long chain family member 5 (ACSL5), transcript variant 2, mRNA.

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CloneID	OHu02964
Clone ID Related Accession (Same CDS sequence)	NM_203379.1 , NM_203380.1 , NM_203379.2
Accession Version	NM_203379.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	long-chain-fatty-acid--CoA ligase 5 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL157786.13, AK000339.1, CF595490.1, AY358520.1 and AK024932.1. On Jun 2, 2019 this sequence version replaced NM_203379.1. Transcript Variant: This variant (2) differs in the 5' UTR and coding region compared to variant 1. This results in translation initiation from a downstream ATG and an isoform (b) that has a shorter N-terminus compared to isoform a. Both variants 2 and 3 encode isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK000339.1, SRR1660803.91901.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTTTTTA TCTTTAACTT TTTGTTTTCC CCACTTCCGA CCCCGGCGTT GATCTGCATC 
CTGACATTTG GAGCTGCCAT CTTCTTGTGG CTGATCACCA GACCTCAACC CGTCTTACCT 
CTTCTTGACC TGAACAATCA GTCTGTGGGA ATTGAGGGAG GAGCACGGAA GGGGGTTTCC 
CAGAAGAACA ATGACCTAAC AAGTTGCTGC TTCTCAGATG CCAAGACTAT GTATGAGGTT 
TTCCAAAGAG GACTCGCTGT GTCTGACAAT GGGCCCTGCT TGGGATATAG AAAACCAAAC 
CAGCCCTACA GATGGCTATC TTACAAACAG GTGTCTGATA GAGCAGAGTA CCTGGGTTCC 
TGTCTCTTGC ATAAAGGTTA TAAATCATCA CCAGACCAGT TTGTCGGCAT CTTTGCTCAG 
AATAGGCCAG AGTGGATCAT CTCCGAATTG GCTTGTTACA CGTACTCTAT GGTAGCTGTA 
CCTCTGTATG ACACCTTGGG ACCAGAAGCC ATCGTACATA TTGTCAACAA GGCTGATATC 
GCCATGGTGA TCTGTGACAC ACCCCAAAAG GCATTGGTGC TGATAGGGAA TGTAGAGAAA 
GGCTTCACCC CGAGCCTGAA GGTGATCATC CTTATGGACC CCTTTGATGA TGACCTGAAG 
CAAAGAGGGG AGAAGAGTGG AATTGAGATC TTATCCCTAT ATGATGCTGA GAACCTAGGC 
AAAGAGCACT TCAGAAAACC TGTGCCTCCT AGCCCAGAAG ACCTGAGCGT CATCTGCTTC 
ACCAGTGGGA CCACAGGTGA CCCCAAAGGA GCCATGATAA CCCATCAAAA TATTGTTTCA 
AATGCTGCTG CCTTTCTCAA ATGTGTGGAG CATGCTTATG AGCCCACTCC TGATGATGTG 
GCCATATCCT ACCTCCCTCT GGCTCATATG TTTGAGAGGA TTGTACAGGC TGTTGTGTAC 
AGCTGTGGAG CCAGAGTTGG ATTCTTCCAA GGGGATATTC GGTTGCTGGC TGACGACATG 
AAGACTTTGA AGCCCACATT GTTTCCCGCG GTGCCTCGAC TCCTTAACAG GATCTACGAT 
AAGGTACAAA ATGAGGCCAA GACACCCTTG AAGAAGTTCT TGTTGAAGCT GGCTGTTTCC 
AGTAAATTCA AAGAGCTTCA AAAGGGTATC ATCAGGCATG ATAGTTTCTG GGACAAGCTC 
ATCTTTGCAA AGATCCAGGA CAGCCTGGGC GGAAGGGTTC GTGTAATTGT CACTGGAGCT 
GCCCCCATGT CCACTTCAGT CATGACATTC TTCCGGGCAG CAATGGGATG TCAGGTGTAT 
GAAGCTTATG GTCAAACAGA ATGCACAGGT GGCTGTACAT TTACATTACC TGGGGACTGG 
ACATCAGGTC ACGTTGGGGT GCCCCTGGCT TGCAATTACG TGAAGCTGGA AGATGTGGCT 
GACATGAACT ACTTTACAGT GAATAATGAA GGAGAGGTCT GCATCAAGGG TACAAACGTG 
TTCAAAGGAT ACCTGAAGGA CCCTGAGAAG ACACAGGAAG CCCTGGACAG TGATGGCTGG 
CTTCACACAG GAGACATTGG TCGCTGGCTC CCGAATGGAA CTCTGAAGAT CATCGACCGT 
AAAAAGAACA TTTTCAAGCT GGCCCAAGGA GAATACATTG CACCAGAGAA GATAGAAAAT 
ATCTACAACA GGAGTCAACC AGTGTTACAA ATTTTTGTAC ACGGGGAGAG CTTACGGTCA 
TCCTTAGTAG GAGTGGTGGT TCCTGACACA GATGTACTTC CCTCATTTGC AGCCAAGCTT 
GGGGTGAAGG GCTCCTTTGA GGAACTGTGC CAAAACCAAG TTGTAAGGGA AGCCATTTTA 
GAAGACTTGC AGAAAATTGG GAAAGAAAGT GGCCTTAAAA CTTTTGAACA GGTCAAAGCC 
ATTTTTCTTC ATCCAGAGCC ATTTTCCATT GAAAATGGGC TCTTGACACC AACATTGAAA 
GCAAAGCGAG GAGAGCTTTC CAAATACTTT CGGACCCAAA TTGACAGCCT GTATGAGCAC 
ATCCAGGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_976313.1
CDS	184..2235
Translation	MLFIFNFLFSPLPTPALICILTFGAAIFLWLITRPQPVLPLLDLNNQSVGIEGGARKGVSQKNNDLTSCCFSDAKTMYEVFQRGLAVSDNGPCLGYRKPNQPYRWLSYKQVSDRAEYLGSCLLHKGYKSSPDQFVGIFAQNRPEWIISELACYTYSMVAVPLYDTLGPEAIVHIVNKADIAMVICDTPQKALVLIGNVEKGFTPSLKVIILMDPFDDDLKQRGEKSGIEILSLYDAENLGKEHFRKPVPPSPEDLSVICFTSGTTGDPKGAMITHQNIVSNAAAFLKCVEHAYEPTPDDVAISYLPLAHMFERIVQAVVYSCGARVGFFQGDIRLLADDMKTLKPTLFPAVPRLLNRIYDKVQNEAKTPLKKFLLKLAVSSKFKELQKGIIRHDSFWDKLIFAKIQDSLGGRVRVIVTGAAPMSTSVMTFFRAAMGCQVYEAYGQTECTGGCTFTLPGDWTSGHVGVPLACNYVKLEDVADMNYFTVNNEGEVCIKGTNVFKGYLKDPEKTQEALDSDGWLHTGDIGRWLPNGTLKIIDRKKNIFKLAQGEYIAPEKIENIYNRSQPVLQIFVHGESLRSSLVGVVVPDTDVLPSFAAKLGVKGSFEELCQNQVVREAILEDLQKIGKESGLKTFEQVKAIFLHPEPFSIENGLLTPTLKAKRGELSKYFRTQIDSLYEHIQD

Target ORF information:

RefSeq Version	NM_203379.2
Organism	Homo sapiens(human)
Definition	Homo sapiens acyl-CoA synthetase long chain family member 5 (ACSL5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_203379.2

ORF Insert Sequence:

ATGCTTTTTA TCTTTAACTT TTTGTTTTCC CCACTTCCGA CCCCGGCGTT GATCTGCATC 
CTGACATTTG GAGCTGCCAT CTTCTTGTGG CTGATCACCA GACCTCAACC CGTCTTACCT 
CTTCTTGACC TGAACAATCA GTCTGTGGGA ATTGAGGGAG GAGCACGGAA GGGGGTTTCC 
CAGAAGAACA ATGACCTAAC AAGTTGCTGC TTCTCAGATG CCAAGACTAT GTATGAGGTT 
TTCCAAAGAG GACTCGCTGT GTCTGACAAT GGGCCCTGCT TGGGATATAG AAAACCAAAC 
CAGCCCTACA GATGGCTATC TTACAAACAG GTGTCTGATA GAGCAGAGTA CCTGGGTTCC 
TGTCTCTTGC ATAAAGGTTA TAAATCATCA CCAGACCAGT TTGTCGGCAT CTTTGCTCAG 
AATAGGCCAG AGTGGATCAT CTCCGAATTG GCTTGTTACA CGTACTCTAT GGTAGCTGTA 
CCTCTGTATG ACACCTTGGG ACCAGAAGCC ATCGTACATA TTGTCAACAA GGCTGATATC 
GCCATGGTGA TCTGTGACAC ACCCCAAAAG GCATTGGTGC TGATAGGGAA TGTAGAGAAA 
GGCTTCACCC CGAGCCTGAA GGTGATCATC CTTATGGACC CCTTTGATGA TGACCTGAAG 
CAAAGAGGGG AGAAGAGTGG AATTGAGATC TTATCCCTAT ATGATGCTGA GAACCTAGGC 
AAAGAGCACT TCAGAAAACC TGTGCCTCCT AGCCCAGAAG ACCTGAGCGT CATCTGCTTC 
ACCAGTGGGA CCACAGGTGA CCCCAAAGGA GCCATGATAA CCCATCAAAA TATTGTTTCA 
AATGCTGCTG CCTTTCTCAA ATGTGTGGAG CATGCTTATG AGCCCACTCC TGATGATGTG 
GCCATATCCT ACCTCCCTCT GGCTCATATG TTTGAGAGGA TTGTACAGGC TGTTGTGTAC 
AGCTGTGGAG CCAGAGTTGG ATTCTTCCAA GGGGATATTC GGTTGCTGGC TGACGACATG 
AAGACTTTGA AGCCCACATT GTTTCCCGCG GTGCCTCGAC TCCTTAACAG GATCTACGAT 
AAGGTACAAA ATGAGGCCAA GACACCCTTG AAGAAGTTCT TGTTGAAGCT GGCTGTTTCC 
AGTAAATTCA AAGAGCTTCA AAAGGGTATC ATCAGGCATG ATAGTTTCTG GGACAAGCTC 
ATCTTTGCAA AGATCCAGGA CAGCCTGGGC GGAAGGGTTC GTGTAATTGT CACTGGAGCT 
GCCCCCATGT CCACTTCAGT CATGACATTC TTCCGGGCAG CAATGGGATG TCAGGTGTAT 
GAAGCTTATG GTCAAACAGA ATGCACAGGT GGCTGTACAT TTACATTACC TGGGGACTGG 
ACATCAGGTC ACGTTGGGGT GCCCCTGGCT TGCAATTACG TGAAGCTGGA AGATGTGGCT 
GACATGAACT ACTTTACAGT GAATAATGAA GGAGAGGTCT GCATCAAGGG TACAAACGTG 
TTCAAAGGAT ACCTGAAGGA CCCTGAGAAG ACACAGGAAG CCCTGGACAG TGATGGCTGG 
CTTCACACAG GAGACATTGG TCGCTGGCTC CCGAATGGAA CTCTGAAGAT CATCGACCGT 
AAAAAGAACA TTTTCAAGCT GGCCCAAGGA GAATACATTG CACCAGAGAA GATAGAAAAT 
ATCTACAACA GGAGTCAACC AGTGTTACAA ATTTTTGTAC ACGGGGAGAG CTTACGGTCA 
TCCTTAGTAG GAGTGGTGGT TCCTGACACA GATGTACTTC CCTCATTTGC AGCCAAGCTT 
GGGGTGAAGG GCTCCTTTGA GGAACTGTGC CAAAACCAAG TTGTAAGGGA AGCCATTTTA 
GAAGACTTGC AGAAAATTGG GAAAGAAAGT GGCCTTAAAA CTTTTGAACA GGTCAAAGCC 
ATTTTTCTTC ATCCAGAGCC ATTTTCCATT GAAAATGGGC TCTTGACACC AACATTGAAA 
GCAAAGCGAG GAGAGCTTTC CAAATACTTT CGGACCCAAA TTGACAGCCT GTATGAGCAC 
ATCCAGGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACSL5 ( XM_017016324.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871813.1 Homo sapiens acyl-CoA synthetase long chain family member 5 (ACSL5), transcript variant X1, mRNA.

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CloneID	OHu91462
Clone ID Related Accession (Same CDS sequence)	XM_017016324.2 , XM_017016324.1
Accession Version	XM_017016324.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	long-chain-fatty-acid--CoA ligase 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017016324.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGCTC TGAAGCCACC CTGTCTCTGG AGGAACCACG AGCGAGGGAA GAAGGACAGG 
GACTCGTGTG GCAGGAAGAA CTCAGAGCCG GGAAGCCCCC ATTCACTAGA AGCACTGAGA 
GATGCGGCCC CCTCGCAGGG TCTGAATTTC CTGCTGCTGT TCACAAAGAT GCTTTTTATC 
TTTAACTTTT TGTTTTCCCC ACTTCCGACC CCGGCGTTGA TCTGCATCCT GACATTTGGA 
GCTGCCATCT TCTTGTGGCT GATCACCAGA CCTCAACCCG TCTTACCTCT TCTTGACCTG 
AACAATCAGT CTGTGGGAAT TGAGGGAGGA GCACGGAAGG GGGTTTCCCA GAAGAACAAT 
GACCTAACAA GTTGCTGCTT CTCAGATGCC AAGACTATGT ATGAGGTTTT CCAAAGAGGA 
CTCGCTGTGT CTGACAATGG GCCCTGCTTG GGATATAGAA AACCAAACCA GCCCTACAGA 
TGGCTATCTT ACAAACAGGT GTCTGATAGA GCAGAGTACC TGGGTTCCTG TCTCTTGCAT 
AAAGGTTATA AATCATCACC AGACCAGTTT GTCGGCATCT TTGCTCAGAA TAGGCCAGAG 
TGGATCATCT CCGAATTGGC TTGTTACACG TACTCTATGG TAGCTGTACC TCTGTATGAC 
ACCTTGGGAC CAGAAGCCAT CGTACATATT GTCAACAAGG CTGATATCGC CATGGTGATC 
TGTGACACAC CCCAAAAGGC ATTGGTGCTG ATAGGGAATG TAGAGAAAGG CTTCACCCCG 
AGCCTGAAGG TGATCATCCT TATGGACCCC TTTGATGATG ACCTGAAGCA AAGAGGGGAG 
AAGAGTGGAA TTGAGATCTT ATCCCTATAT GATGCTGAGA ACCTAGGCAA AGAGCACTTC 
AGAAAACCTG TGCCTCCTAG CCCAGAAGAC CTGAGCGTCA TCTGCTTCAC CAGTGGGACC 
ACAGGTGACC CCAAAGGAGC CATGATAACC CATCAAAATA TTGTTTCAAA TGCTGCTGCC 
TTTCTCAAAT GTGTGGAGCA TGCTTATGAG CCCACTCCTG ATGATGTGGC CATATCCTAC 
CTCCCTCTGG CTCATATGTT TGAGAGGATT GTACAGGCTG TTGTGTACAG CTGTGGAGCC 
AGAGTTGGAT TCTTCCAAGG GGATATTCGG TTGCTGGCTG ACGACATGAA GACTTTGAAG 
CCCACATTGT TTCCCGCGGT GCCTCGACTC CTTAACAGGA TCTACGATAA GGTACAAAAT 
GAGGCCAAGA CACCCTTGAA GAAGTTCTTG TTGAAGCTGG CTGTTTCCAG TAAATTCAAA 
GAGCTTCAAA AGGGTATCAT CAGGCATGAT AGTTTCTGGG ACAAGCTCAT CTTTGCAAAG 
ATCCAGGACA GCCTGGGCGG AAGGGTTCGT GTAATTGTCA CTGGAGCTGC CCCCATGTCC 
ACTTCAGTCA TGACATTCTT CCGGGCAGCA ATGGGATGTC AGGTGTATGA AGCTTATGGT 
CAAACAGAAT GCACAGGTGG CTGTACATTT ACATTACCTG GGGACTGGAC ATCAGGTCAC 
GTTGGGGTGC CCCTGGCTTG CAATTACGTG AAGCTGGAAG ATGTGGCTGA CATGAACTAC 
TTTACAGTGA ATAATGAAGG AGAGGTCTGC ATCAAGGGTA CAAACGTGTT CAAAGGATAC 
CTGAAGGACC CTGAGAAGAC ACAGGAAGCC CTGGACAGTG ATGGCTGGCT TCACACAGGA 
GACATTGGTC GCTGGCTCCC GAATGGAACT CTGAAGATCA TCGACCGTAA AAAGAACATT 
TTCAAGCTGG CCCAAGGAGA ATACATTGCA CCAGAGAAGA TAGAAAATAT CTACAACAGG 
AGTCAACCAG TGTTACAAAT TTTTGTACAC GGGGAGAGCT TACGGTCATC CTTAGTAGGA 
GTGGTGGTTC CTGACACAGA TGTACTTCCC TCATTTGCAG CCAAGCTTGG GGTGAAGGGC 
TCCTTTGAGG AACTGTGCCA AAACCAAGTC AAAGCCATTT TTCTTCATCC AGAGCCATTT 
TCCATTGAAA ATGGGCTCTT GACACCAACA TTGAAAGCAA AGCGAGGAGA GCTTTCCAAA 
TACTTTCGGA CCCAAATTGA CAGCCTGTAT GAGCACATCC AGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871813.1
CDS	112..2259
Translation	MDALKPPCLWRNHERGKKDRDSCGRKNSEPGSPHSLEALRDAAPSQGLNFLLLFTKMLFIFNFLFSPLPTPALICILTFGAAIFLWLITRPQPVLPLLDLNNQSVGIEGGARKGVSQKNNDLTSCCFSDAKTMYEVFQRGLAVSDNGPCLGYRKPNQPYRWLSYKQVSDRAEYLGSCLLHKGYKSSPDQFVGIFAQNRPEWIISELACYTYSMVAVPLYDTLGPEAIVHIVNKADIAMVICDTPQKALVLIGNVEKGFTPSLKVIILMDPFDDDLKQRGEKSGIEILSLYDAENLGKEHFRKPVPPSPEDLSVICFTSGTTGDPKGAMITHQNIVSNAAAFLKCVEHAYEPTPDDVAISYLPLAHMFERIVQAVVYSCGARVGFFQGDIRLLADDMKTLKPTLFPAVPRLLNRIYDKVQNEAKTPLKKFLLKLAVSSKFKELQKGIIRHDSFWDKLIFAKIQDSLGGRVRVIVTGAAPMSTSVMTFFRAAMGCQVYEAYGQTECTGGCTFTLPGDWTSGHVGVPLACNYVKLEDVADMNYFTVNNEGEVCIKGTNVFKGYLKDPEKTQEALDSDGWLHTGDIGRWLPNGTLKIIDRKKNIFKLAQGEYIAPEKIENIYNRSQPVLQIFVHGESLRSSLVGVVVPDTDVLPSFAAKLGVKGSFEELCQNQVKAIFLHPEPFSIENGLLTPTLKAKRGELSKYFRTQIDSLYEHIQD

Target ORF information:

RefSeq Version	XM_017016324.2
Organism	Homo sapiens(human)
Definition	Homo sapiens acyl-CoA synthetase long chain family member 5 (ACSL5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016324.2

ORF Insert Sequence:

ATGGACGCTC TGAAGCCACC CTGTCTCTGG AGGAACCACG AGCGAGGGAA GAAGGACAGG 
GACTCGTGTG GCAGGAAGAA CTCAGAGCCG GGAAGCCCCC ATTCACTAGA AGCACTGAGA 
GATGCGGCCC CCTCGCAGGG TCTGAATTTC CTGCTGCTGT TCACAAAGAT GCTTTTTATC 
TTTAACTTTT TGTTTTCCCC ACTTCCGACC CCGGCGTTGA TCTGCATCCT GACATTTGGA 
GCTGCCATCT TCTTGTGGCT GATCACCAGA CCTCAACCCG TCTTACCTCT TCTTGACCTG 
AACAATCAGT CTGTGGGAAT TGAGGGAGGA GCACGGAAGG GGGTTTCCCA GAAGAACAAT 
GACCTAACAA GTTGCTGCTT CTCAGATGCC AAGACTATGT ATGAGGTTTT CCAAAGAGGA 
CTCGCTGTGT CTGACAATGG GCCCTGCTTG GGATATAGAA AACCAAACCA GCCCTACAGA 
TGGCTATCTT ACAAACAGGT GTCTGATAGA GCAGAGTACC TGGGTTCCTG TCTCTTGCAT 
AAAGGTTATA AATCATCACC AGACCAGTTT GTCGGCATCT TTGCTCAGAA TAGGCCAGAG 
TGGATCATCT CCGAATTGGC TTGTTACACG TACTCTATGG TAGCTGTACC TCTGTATGAC 
ACCTTGGGAC CAGAAGCCAT CGTACATATT GTCAACAAGG CTGATATCGC CATGGTGATC 
TGTGACACAC CCCAAAAGGC ATTGGTGCTG ATAGGGAATG TAGAGAAAGG CTTCACCCCG 
AGCCTGAAGG TGATCATCCT TATGGACCCC TTTGATGATG ACCTGAAGCA AAGAGGGGAG 
AAGAGTGGAA TTGAGATCTT ATCCCTATAT GATGCTGAGA ACCTAGGCAA AGAGCACTTC 
AGAAAACCTG TGCCTCCTAG CCCAGAAGAC CTGAGCGTCA TCTGCTTCAC CAGTGGGACC 
ACAGGTGACC CCAAAGGAGC CATGATAACC CATCAAAATA TTGTTTCAAA TGCTGCTGCC 
TTTCTCAAAT GTGTGGAGCA TGCTTATGAG CCCACTCCTG ATGATGTGGC CATATCCTAC 
CTCCCTCTGG CTCATATGTT TGAGAGGATT GTACAGGCTG TTGTGTACAG CTGTGGAGCC 
AGAGTTGGAT TCTTCCAAGG GGATATTCGG TTGCTGGCTG ACGACATGAA GACTTTGAAG 
CCCACATTGT TTCCCGCGGT GCCTCGACTC CTTAACAGGA TCTACGATAA GGTACAAAAT 
GAGGCCAAGA CACCCTTGAA GAAGTTCTTG TTGAAGCTGG CTGTTTCCAG TAAATTCAAA 
GAGCTTCAAA AGGGTATCAT CAGGCATGAT AGTTTCTGGG ACAAGCTCAT CTTTGCAAAG 
ATCCAGGACA GCCTGGGCGG AAGGGTTCGT GTAATTGTCA CTGGAGCTGC CCCCATGTCC 
ACTTCAGTCA TGACATTCTT CCGGGCAGCA ATGGGATGTC AGGTGTATGA AGCTTATGGT 
CAAACAGAAT GCACAGGTGG CTGTACATTT ACATTACCTG GGGACTGGAC ATCAGGTCAC 
GTTGGGGTGC CCCTGGCTTG CAATTACGTG AAGCTGGAAG ATGTGGCTGA CATGAACTAC 
TTTACAGTGA ATAATGAAGG AGAGGTCTGC ATCAAGGGTA CAAACGTGTT CAAAGGATAC 
CTGAAGGACC CTGAGAAGAC ACAGGAAGCC CTGGACAGTG ATGGCTGGCT TCACACAGGA 
GACATTGGTC GCTGGCTCCC GAATGGAACT CTGAAGATCA TCGACCGTAA AAAGAACATT 
TTCAAGCTGG CCCAAGGAGA ATACATTGCA CCAGAGAAGA TAGAAAATAT CTACAACAGG 
AGTCAACCAG TGTTACAAAT TTTTGTACAC GGGGAGAGCT TACGGTCATC CTTAGTAGGA 
GTGGTGGTTC CTGACACAGA TGTACTTCCC TCATTTGCAG CCAAGCTTGG GGTGAAGGGC 
TCCTTTGAGG AACTGTGCCA AAACCAAGTC AAAGCCATTT TTCTTCATCC AGAGCCATTT 
TCCATTGAAA ATGGGCTCTT GACACCAACA TTGAAAGCAA AGCGAGGAGA GCTTTCCAAA 
TACTTTCGGA CCCAAATTGA CAGCCTGTAT GAGCACATCC AGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACSL5 ( XM_017016324.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871813.1 Homo sapiens acyl-CoA synthetase long-chain family member 5 (ACSL5), transcript variant X1, mRNA.

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CloneID	OHu91462
Clone ID Related Accession (Same CDS sequence)	XM_017016324.2 , XM_017016324.1
Accession Version	XM_017016324.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	long-chain-fatty-acid--CoA ligase 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGCTC TGAAGCCACC CTGTCTCTGG AGGAACCACG AGCGAGGGAA GAAGGACAGG 
GACTCGTGTG GCAGGAAGAA CTCAGAGCCG GGAAGCCCCC ATTCACTAGA AGCACTGAGA 
GATGCGGCCC CCTCGCAGGG TCTGAATTTC CTGCTGCTGT TCACAAAGAT GCTTTTTATC 
TTTAACTTTT TGTTTTCCCC ACTTCCGACC CCGGCGTTGA TCTGCATCCT GACATTTGGA 
GCTGCCATCT TCTTGTGGCT GATCACCAGA CCTCAACCCG TCTTACCTCT TCTTGACCTG 
AACAATCAGT CTGTGGGAAT TGAGGGAGGA GCACGGAAGG GGGTTTCCCA GAAGAACAAT 
GACCTAACAA GTTGCTGCTT CTCAGATGCC AAGACTATGT ATGAGGTTTT CCAAAGAGGA 
CTCGCTGTGT CTGACAATGG GCCCTGCTTG GGATATAGAA AACCAAACCA GCCCTACAGA 
TGGCTATCTT ACAAACAGGT GTCTGATAGA GCAGAGTACC TGGGTTCCTG TCTCTTGCAT 
AAAGGTTATA AATCATCACC AGACCAGTTT GTCGGCATCT TTGCTCAGAA TAGGCCAGAG 
TGGATCATCT CCGAATTGGC TTGTTACACG TACTCTATGG TAGCTGTACC TCTGTATGAC 
ACCTTGGGAC CAGAAGCCAT CGTACATATT GTCAACAAGG CTGATATCGC CATGGTGATC 
TGTGACACAC CCCAAAAGGC ATTGGTGCTG ATAGGGAATG TAGAGAAAGG CTTCACCCCG 
AGCCTGAAGG TGATCATCCT TATGGACCCC TTTGATGATG ACCTGAAGCA AAGAGGGGAG 
AAGAGTGGAA TTGAGATCTT ATCCCTATAT GATGCTGAGA ACCTAGGCAA AGAGCACTTC 
AGAAAACCTG TGCCTCCTAG CCCAGAAGAC CTGAGCGTCA TCTGCTTCAC CAGTGGGACC 
ACAGGTGACC CCAAAGGAGC CATGATAACC CATCAAAATA TTGTTTCAAA TGCTGCTGCC 
TTTCTCAAAT GTGTGGAGCA TGCTTATGAG CCCACTCCTG ATGATGTGGC CATATCCTAC 
CTCCCTCTGG CTCATATGTT TGAGAGGATT GTACAGGCTG TTGTGTACAG CTGTGGAGCC 
AGAGTTGGAT TCTTCCAAGG GGATATTCGG TTGCTGGCTG ACGACATGAA GACTTTGAAG 
CCCACATTGT TTCCCGCGGT GCCTCGACTC CTTAACAGGA TCTACGATAA GGTACAAAAT 
GAGGCCAAGA CACCCTTGAA GAAGTTCTTG TTGAAGCTGG CTGTTTCCAG TAAATTCAAA 
GAGCTTCAAA AGGGTATCAT CAGGCATGAT AGTTTCTGGG ACAAGCTCAT CTTTGCAAAG 
ATCCAGGACA GCCTGGGCGG AAGGGTTCGT GTAATTGTCA CTGGAGCTGC CCCCATGTCC 
ACTTCAGTCA TGACATTCTT CCGGGCAGCA ATGGGATGTC AGGTGTATGA AGCTTATGGT 
CAAACAGAAT GCACAGGTGG CTGTACATTT ACATTACCTG GGGACTGGAC ATCAGGTCAC 
GTTGGGGTGC CCCTGGCTTG CAATTACGTG AAGCTGGAAG ATGTGGCTGA CATGAACTAC 
TTTACAGTGA ATAATGAAGG AGAGGTCTGC ATCAAGGGTA CAAACGTGTT CAAAGGATAC 
CTGAAGGACC CTGAGAAGAC ACAGGAAGCC CTGGACAGTG ATGGCTGGCT TCACACAGGA 
GACATTGGTC GCTGGCTCCC GAATGGAACT CTGAAGATCA TCGACCGTAA AAAGAACATT 
TTCAAGCTGG CCCAAGGAGA ATACATTGCA CCAGAGAAGA TAGAAAATAT CTACAACAGG 
AGTCAACCAG TGTTACAAAT TTTTGTACAC GGGGAGAGCT TACGGTCATC CTTAGTAGGA 
GTGGTGGTTC CTGACACAGA TGTACTTCCC TCATTTGCAG CCAAGCTTGG GGTGAAGGGC 
TCCTTTGAGG AACTGTGCCA AAACCAAGTC AAAGCCATTT TTCTTCATCC AGAGCCATTT 
TCCATTGAAA ATGGGCTCTT GACACCAACA TTGAAAGCAA AGCGAGGAGA GCTTTCCAAA 
TACTTTCGGA CCCAAATTGA CAGCCTGTAT GAGCACATCC AGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871813.1
CDS	148..2295
Translation	MDALKPPCLWRNHERGKKDRDSCGRKNSEPGSPHSLEALRDAAPSQGLNFLLLFTKMLFIFNFLFSPLPTPALICILTFGAAIFLWLITRPQPVLPLLDLNNQSVGIEGGARKGVSQKNNDLTSCCFSDAKTMYEVFQRGLAVSDNGPCLGYRKPNQPYRWLSYKQVSDRAEYLGSCLLHKGYKSSPDQFVGIFAQNRPEWIISELACYTYSMVAVPLYDTLGPEAIVHIVNKADIAMVICDTPQKALVLIGNVEKGFTPSLKVIILMDPFDDDLKQRGEKSGIEILSLYDAENLGKEHFRKPVPPSPEDLSVICFTSGTTGDPKGAMITHQNIVSNAAAFLKCVEHAYEPTPDDVAISYLPLAHMFERIVQAVVYSCGARVGFFQGDIRLLADDMKTLKPTLFPAVPRLLNRIYDKVQNEAKTPLKKFLLKLAVSSKFKELQKGIIRHDSFWDKLIFAKIQDSLGGRVRVIVTGAAPMSTSVMTFFRAAMGCQVYEAYGQTECTGGCTFTLPGDWTSGHVGVPLACNYVKLEDVADMNYFTVNNEGEVCIKGTNVFKGYLKDPEKTQEALDSDGWLHTGDIGRWLPNGTLKIIDRKKNIFKLAQGEYIAPEKIENIYNRSQPVLQIFVHGESLRSSLVGVVVPDTDVLPSFAAKLGVKGSFEELCQNQVKAIFLHPEPFSIENGLLTPTLKAKRGELSKYFRTQIDSLYEHIQD

Target ORF information:

RefSeq Version	XM_017016324.1
Organism	Homo sapiens(human)
Definition	Homo sapiens acyl-CoA synthetase long-chain family member 5 (ACSL5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016324.1

ORF Insert Sequence:

ATGGACGCTC TGAAGCCACC CTGTCTCTGG AGGAACCACG AGCGAGGGAA GAAGGACAGG 
GACTCGTGTG GCAGGAAGAA CTCAGAGCCG GGAAGCCCCC ATTCACTAGA AGCACTGAGA 
GATGCGGCCC CCTCGCAGGG TCTGAATTTC CTGCTGCTGT TCACAAAGAT GCTTTTTATC 
TTTAACTTTT TGTTTTCCCC ACTTCCGACC CCGGCGTTGA TCTGCATCCT GACATTTGGA 
GCTGCCATCT TCTTGTGGCT GATCACCAGA CCTCAACCCG TCTTACCTCT TCTTGACCTG 
AACAATCAGT CTGTGGGAAT TGAGGGAGGA GCACGGAAGG GGGTTTCCCA GAAGAACAAT 
GACCTAACAA GTTGCTGCTT CTCAGATGCC AAGACTATGT ATGAGGTTTT CCAAAGAGGA 
CTCGCTGTGT CTGACAATGG GCCCTGCTTG GGATATAGAA AACCAAACCA GCCCTACAGA 
TGGCTATCTT ACAAACAGGT GTCTGATAGA GCAGAGTACC TGGGTTCCTG TCTCTTGCAT 
AAAGGTTATA AATCATCACC AGACCAGTTT GTCGGCATCT TTGCTCAGAA TAGGCCAGAG 
TGGATCATCT CCGAATTGGC TTGTTACACG TACTCTATGG TAGCTGTACC TCTGTATGAC 
ACCTTGGGAC CAGAAGCCAT CGTACATATT GTCAACAAGG CTGATATCGC CATGGTGATC 
TGTGACACAC CCCAAAAGGC ATTGGTGCTG ATAGGGAATG TAGAGAAAGG CTTCACCCCG 
AGCCTGAAGG TGATCATCCT TATGGACCCC TTTGATGATG ACCTGAAGCA AAGAGGGGAG 
AAGAGTGGAA TTGAGATCTT ATCCCTATAT GATGCTGAGA ACCTAGGCAA AGAGCACTTC 
AGAAAACCTG TGCCTCCTAG CCCAGAAGAC CTGAGCGTCA TCTGCTTCAC CAGTGGGACC 
ACAGGTGACC CCAAAGGAGC CATGATAACC CATCAAAATA TTGTTTCAAA TGCTGCTGCC 
TTTCTCAAAT GTGTGGAGCA TGCTTATGAG CCCACTCCTG ATGATGTGGC CATATCCTAC 
CTCCCTCTGG CTCATATGTT TGAGAGGATT GTACAGGCTG TTGTGTACAG CTGTGGAGCC 
AGAGTTGGAT TCTTCCAAGG GGATATTCGG TTGCTGGCTG ACGACATGAA GACTTTGAAG 
CCCACATTGT TTCCCGCGGT GCCTCGACTC CTTAACAGGA TCTACGATAA GGTACAAAAT 
GAGGCCAAGA CACCCTTGAA GAAGTTCTTG TTGAAGCTGG CTGTTTCCAG TAAATTCAAA 
GAGCTTCAAA AGGGTATCAT CAGGCATGAT AGTTTCTGGG ACAAGCTCAT CTTTGCAAAG 
ATCCAGGACA GCCTGGGCGG AAGGGTTCGT GTAATTGTCA CTGGAGCTGC CCCCATGTCC 
ACTTCAGTCA TGACATTCTT CCGGGCAGCA ATGGGATGTC AGGTGTATGA AGCTTATGGT 
CAAACAGAAT GCACAGGTGG CTGTACATTT ACATTACCTG GGGACTGGAC ATCAGGTCAC 
GTTGGGGTGC CCCTGGCTTG CAATTACGTG AAGCTGGAAG ATGTGGCTGA CATGAACTAC 
TTTACAGTGA ATAATGAAGG AGAGGTCTGC ATCAAGGGTA CAAACGTGTT CAAAGGATAC 
CTGAAGGACC CTGAGAAGAC ACAGGAAGCC CTGGACAGTG ATGGCTGGCT TCACACAGGA 
GACATTGGTC GCTGGCTCCC GAATGGAACT CTGAAGATCA TCGACCGTAA AAAGAACATT 
TTCAAGCTGG CCCAAGGAGA ATACATTGCA CCAGAGAAGA TAGAAAATAT CTACAACAGG 
AGTCAACCAG TGTTACAAAT TTTTGTACAC GGGGAGAGCT TACGGTCATC CTTAGTAGGA 
GTGGTGGTTC CTGACACAGA TGTACTTCCC TCATTTGCAG CCAAGCTTGG GGTGAAGGGC 
TCCTTTGAGG AACTGTGCCA AAACCAAGTC AAAGCCATTT TTCTTCATCC AGAGCCATTT 
TCCATTGAAA ATGGGCTCTT GACACCAACA TTGAAAGCAA AGCGAGGAGA GCTTTCCAAA 
TACTTTCGGA CCCAAATTGA CAGCCTGTAT GAGCACATCC AGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Association of long-chain acyl-coenzyme A synthetase?5 expression in human breast cancer by estrogen receptor status and its clinical significance.
Oncology reports37(6)3253-3260(2017 Jun)
Yen MC,Kan JY,Hsieh CJ,Kuo PL,Hou MF,Hsu YL

Low acyl-CoA synthetase 5 expression in colorectal carcinomas is prognostic for early tumour recurrence.
Pathology, research and practice213(3)261-266(2017 Mar)
Hartmann F,Sparla D,Tute E,Tamm M,Schneider U,Jeon MK,Kasperk R,Gassler N,Kaemmerer E

Acyl-CoA Synthetase 5 Promotes the Growth and Invasion of Colorectal Cancer Cells.
Canadian journal of gastroenterology & hepatology20177615736(2017)
Ding S,Tang S,Wang M,Wu D,Guo H

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ACSL5 cDNA ORF clone, Homo sapiens(human)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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