The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in uterus and spleen, and in trace amounts in normal brain, but has markedly increased levels in malignant gliomas. This gene functions in mediating fatty acid-induced glioma cell growth. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
The following ACSL5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACSL5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL708480.1, AY358520.1, AK024932.1 and AK000339.1. On Feb 25, 2004 this sequence version replaced NM_016234.2.
Transcript Variant: This variant (1) encodes the longer isoform (a).
Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: AK024932.1, SRR1803615.70193.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348]
##Evidence-Data-END##
COMPLETENESS: complete on the 3' end.
Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK000339.1, CF595490.1, AY358520.1 and AK024932.1.
Transcript Variant: This variant (2) differs in the 5' UTR and coding region compared to variant 1. This results in translation initiation from a downstream ATG and an isoform (b) that has a shorter N-terminus compared to isoform a. Both variants 2 and 3 encode isoform b.
Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: AK000339.1, SRR1660803.91901.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348]
##Evidence-Data-END##
COMPLETENESS: complete on the 3' end.
Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD689921.1, AL708480.1, AY358520.1, AK024932.1 and AK000339.1.
Transcript Variant: This variant (3) differs in the 5' UTR and coding region compared to variant 1. This results in translation initiation from a downstream ATG and an isoform (b) that has a shorter N-terminus compared to isoform a. Both variants 2 and 3 encode isoform b.
Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: SRR1803611.279490.1, SRR1660805.116816.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348]
##Evidence-Data-END##
COMPLETENESS: complete on the 3' end.
Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL157786.13, AK000339.1, CF595490.1, AY358520.1 and AK024932.1. On Jun 2, 2019 this sequence version replaced NM_203379.1.
Transcript Variant: This variant (2) differs in the 5' UTR and coding region compared to variant 1. This results in translation initiation from a downstream ATG and an isoform (b) that has a shorter N-terminus compared to isoform a. Both variants 2 and 3 encode isoform b.
Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: AK000339.1, SRR1660803.91901.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348]
##Evidence-Data-END##
##RefSeq-Attributes-START##
RefSeq Select criteria :: based on conservation, expression
##RefSeq-Attributes-END##
COMPLETENESS: complete on the 3' end.
Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process
On Mar 26, 2018 this sequence version replaced XM_017016324.1.
##Genome-Annotation-Data-START##
Annotation Provider :: NCBI
Annotation Status :: Updated annotation
Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205
Annotation Version :: 109.20191205
Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline
Annotation Software Version :: 8.3
Annotation Method :: Best-placed RefSeq; propagated RefSeq model
Features Annotated :: Gene; mRNA; CDS; ncRNA
##Genome-Annotation-Data-END##
Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process
##Genome-Annotation-Data-START##
Annotation Provider :: NCBI
Annotation Status :: Full annotation
Annotation Version :: Homo sapiens Annotation Release 108
Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline
Annotation Software Version :: 7.0
Annotation Method :: Best-placed RefSeq; Gnomon
Features Annotated :: Gene; mRNA; CDS; ncRNA
##Genome-Annotation-Data-END##
User Manual
NM_016234.3
