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PENK cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol PENK
Entrez Gene ID 5179
Full Name proenkephalin
Synonyms PE,PENK-A
General protein information
Preferred Names

proenkephalin

Names

proenkephalin-A
enkephalin A
peptide F
preproenkephalin
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

8

8q12.1
Summary This gene encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include the pentapeptide opioids Met-enkephalin and Leu-enkephalin, which are stored in synaptic vesicles, then released into the synapse where they bind to mu- and delta-opioid receptors to modulate the perception of pain. Other non-opioid cleavage products may function in distinct biological activities. [provided by RefSeq, Jul 2015].
Disorder MIM:

131330


Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001135690.3 NP_001129162.1 proenkephalin-A preproprotein
NM_001135690.2 NP_001129162.1 proenkephalin-A preproprotein

Pathways from BioSystems


Homologies


Mus musculus (house mouse) Penk NP_001002927.1
Rattus norvegicus (Norway rat) Penk NP_058835.1
Danio rerio (zebrafish) penka NP_956377.1
Gallus gallus (chicken) PENK XP_419213.1
Xenopus tropicalis (tropical clawed frog) penk NP_001015744.2
Homo sapiens (human) PENK NP_006202.1
Pan troglodytes (chimpanzee) PENK XP_001155627.1
LOC696649 XP_002808525.1
Canis lupus familiaris (dog) PENK XP_003640059.2
Bos taurus (cattle) PENK NP_776566.1

Gene Ontology


Bibliography

Related articles in PubMed

Elevation of Proenkephalin 143-183 in Cerebrospinal Fluid in Moyamoya Disease.
Yokoyama K, Maruwaka M, Yoshikawa K, Araki Y, Okamoto S, Sumitomo M, Kawamura A, Sakamoto Y, Shimizu K, Izumi T, Wakabayashi T
World neurosurgery109e446-e459(2018 Jan)

The effects of different exercise training modalities on plasma proenkephalin Peptide F in women.
DuPont WH, Kraemer WJ, Nindl BC, Lee EC, Fragala MS, Hatfield DL, Caldwell LK, Post EM, Beeler MK, Volek JS, Maresh CM
Peptides9126-32(2017 May)

Endogenous opioids contribute to insensitivity to pain in humans and mice lacking sodium channel Nav1.7.
Minett MS, Pereira V, Sikandar S, Matsuyama A, Lolignier S, Kanellopoulos AH, Mancini F, Iannetti GD, Bogdanov YD, Santana-Varela S, Millet Q, Baskozos G, MacAllister R, Cox JJ, Zhao J, Wood JN
Nature communications68967(2015 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The concentration of PENK 143-183 was significantly greater in patients with Moyamoya disease (MMD) than control patients and decreased in an age-dependent manner in MMD.
Title: Elevation of Proenkephalin 143-183 in Cerebrospinal Fluid in Moyamoya Disease.

Nav1.7 deletion has profound effects on gene expression, leading to an upregulation of enkephalin precursor Penk mRNA and met-enkephalin protein in sensory neurons.
Title: Endogenous opioids contribute to insensitivity to pain in humans and mice lacking sodium channel Nav1.7.

The PENK polymorphism-and potentially opioid neurotransmission more generally-modulates functioning and structural integrity of brain regions.
Title: Effects of an opioid (proenkephalin) polymorphism on neural response to errors in health and cocaine use disorder.

BSP demonstrated that emodin, to a certain degree, affected the demethylation of tumor-suppressor genes P16, RASSF1A, and ppENK in the pancreatic cancer cell line PANC-1.
Title: Effects of emodin on the demethylation of tumor-suppressor genes in pancreatic cancer PANC-1 cells.

Serum PENK-A levels were not associated with all-cause mortality in patients with type 2 diabetes mellitus, and its association with cardiovascular mortality was strongly attenuated after accounting for all traditional risk factors.
Title: Serum Proenkephalin A Levels and Mortality After Long-Term Follow-Up in Patients with Type 2 Diabetes Mellitus (ZODIAC-32).

The following PENK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PENK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu23531 NM_001135690.3
Latest version!		 Homo sapiens proenkephalin (PENK), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $216.30
$309.00
View Old Accession Versions >>
OHu23531 NM_001135690.2 Homo sapiens proenkephalin (PENK), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $216.30
$309.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu23531
Clone ID Related Accession (Same CDS sequence) NM_001135690.3 , NM_001135690.2
Accession Version NM_001135690.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 804bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Homo sapiens(human)
Product proenkephalin-A preproprotein
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK314908.1 and BI496610.1. On Nov 24, 2018 this sequence version replaced NM_001135690.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189667.173079.1, SRR1660805.88477.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000451791.7/ ENSP00000400894.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCGCGGT TCCTGACACT TTGCACTTGG CTGCTGTTGC TCGGCCCCGG GCTCCTGGCG 
ACCGTGCGGG CCGAATGCAG CCAGGATTGC GCGACGTGCA GCTACCGCCT AGTGCGCCCG 
GCCGACATCA ACTTCCTGGC TTGCGTAATG GAATGTGAAG GTAAACTGCC TTCTCTGAAA 
ATTTGGGAAA CCTGCAAGGA GCTCCTGCAG CTGTCCAAAC CAGAGCTTCC TCAAGATGGC 
ACCAGCACCC TCAGAGAAAA TAGCAAACCG GAAGAAAGCC ATTTGCTAGC CAAAAGGTAT 
GGGGGCTTCA TGAAAAGGTA TGGAGGCTTC ATGAAGAAAA TGGATGAGCT TTATCCCATG 
GAGCCAGAAG AAGAGGCCAA TGGAAGTGAG ATCCTCGCCA AGCGGTATGG GGGCTTCATG 
AAGAAGGATG CAGAGGAGGA CGACTCGCTG GCCAATTCCT CAGACCTGCT AAAAGAGCTT 
CTGGAAACAG GGGACAACCG AGAGCGTAGC CACCACCAGG ATGGCAGTGA TAATGAGGAA 
GAAGTGAGCA AGAGATATGG GGGCTTCATG AGAGGCTTAA AGAGAAGCCC CCAACTGGAA 
GATGAAGCCA AAGAGCTGCA GAAGCGATAT GGGGGCTTCA TGAGAAGAGT AGGTCGCCCA 
GAGTGGTGGA TGGACTACCA GAAACGGTAT GGAGGTTTCC TGAAGCGCTT TGCCGAGGCT 
CTGCCCTCCG ACGAAGAAGG CGAAAGTTAC TCCAAAGAAG TTCCTGAAAT GGAAAAAAGA 
TACGGAGGAT TTATGAGATT TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001129162.1
CDS133..936
Translation

Target ORF information:

RefSeq Version NM_001135690.3
Organism Homo sapiens(human)
Definition Homo sapiens proenkephalin (PENK), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001135690.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCGCGGT TCCTGACACT TTGCACTTGG CTGCTGTTGC TCGGCCCCGG GCTCCTGGCG 
ACCGTGCGGG CCGAATGCAG CCAGGATTGC GCGACGTGCA GCTACCGCCT AGTGCGCCCG 
GCCGACATCA ACTTCCTGGC TTGCGTAATG GAATGTGAAG GTAAACTGCC TTCTCTGAAA 
ATTTGGGAAA CCTGCAAGGA GCTCCTGCAG CTGTCCAAAC CAGAGCTTCC TCAAGATGGC 
ACCAGCACCC TCAGAGAAAA TAGCAAACCG GAAGAAAGCC ATTTGCTAGC CAAAAGGTAT 
GGGGGCTTCA TGAAAAGGTA TGGAGGCTTC ATGAAGAAAA TGGATGAGCT TTATCCCATG 
GAGCCAGAAG AAGAGGCCAA TGGAAGTGAG ATCCTCGCCA AGCGGTATGG GGGCTTCATG 
AAGAAGGATG CAGAGGAGGA CGACTCGCTG GCCAATTCCT CAGACCTGCT AAAAGAGCTT 
CTGGAAACAG GGGACAACCG AGAGCGTAGC CACCACCAGG ATGGCAGTGA TAATGAGGAA 
GAAGTGAGCA AGAGATATGG GGGCTTCATG AGAGGCTTAA AGAGAAGCCC CCAACTGGAA 
GATGAAGCCA AAGAGCTGCA GAAGCGATAT GGGGGCTTCA TGAGAAGAGT AGGTCGCCCA 
GAGTGGTGGA TGGACTACCA GAAACGGTAT GGAGGTTTCC TGAAGCGCTT TGCCGAGGCT 
CTGCCCTCCG ACGAAGAAGG CGAAAGTTAC TCCAAAGAAG TTCCTGAAAT GGAAAAAAGA 
TACGGAGGAT TTATGAGATT TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23531
Clone ID Related Accession (Same CDS sequence) NM_001135690.3 , NM_001135690.2
Accession Version NM_001135690.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 804bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-12-31
Organism Homo sapiens(human)
Product proenkephalin-A preproprotein
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP370091.1, DB496514.1, AK314908.1 and BI496610.1. On Jul 21, 2015 this sequence version replaced NM_001135690.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.88477.1, ERR279837.3641.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCGCGGT TCCTGACACT TTGCACTTGG CTGCTGTTGC TCGGCCCCGG GCTCCTGGCG 
ACCGTGCGGG CCGAATGCAG CCAGGATTGC GCGACGTGCA GCTACCGCCT AGTGCGCCCG 
GCCGACATCA ACTTCCTGGC TTGCGTAATG GAATGTGAAG GTAAACTGCC TTCTCTGAAA 
ATTTGGGAAA CCTGCAAGGA GCTCCTGCAG CTGTCCAAAC CAGAGCTTCC TCAAGATGGC 
ACCAGCACCC TCAGAGAAAA TAGCAAACCG GAAGAAAGCC ATTTGCTAGC CAAAAGGTAT 
GGGGGCTTCA TGAAAAGGTA TGGAGGCTTC ATGAAGAAAA TGGATGAGCT TTATCCCATG 
GAGCCAGAAG AAGAGGCCAA TGGAAGTGAG ATCCTCGCCA AGCGGTATGG GGGCTTCATG 
AAGAAGGATG CAGAGGAGGA CGACTCGCTG GCCAATTCCT CAGACCTGCT AAAAGAGCTT 
CTGGAAACAG GGGACAACCG AGAGCGTAGC CACCACCAGG ATGGCAGTGA TAATGAGGAA 
GAAGTGAGCA AGAGATATGG GGGCTTCATG AGAGGCTTAA AGAGAAGCCC CCAACTGGAA 
GATGAAGCCA AAGAGCTGCA GAAGCGATAT GGGGGCTTCA TGAGAAGAGT AGGTCGCCCA 
GAGTGGTGGA TGGACTACCA GAAACGGTAT GGAGGTTTCC TGAAGCGCTT TGCCGAGGCT 
CTGCCCTCCG ACGAAGAAGG CGAAAGTTAC TCCAAAGAAG TTCCTGAAAT GGAAAAAAGA 
TACGGAGGAT TTATGAGATT TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001129162.1
CDS226..1029
Translation

Target ORF information:

RefSeq Version NM_001135690.2
Organism Homo sapiens(human)
Definition Homo sapiens proenkephalin (PENK), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001135690.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCGCGGT TCCTGACACT TTGCACTTGG CTGCTGTTGC TCGGCCCCGG GCTCCTGGCG 
ACCGTGCGGG CCGAATGCAG CCAGGATTGC GCGACGTGCA GCTACCGCCT AGTGCGCCCG 
GCCGACATCA ACTTCCTGGC TTGCGTAATG GAATGTGAAG GTAAACTGCC TTCTCTGAAA 
ATTTGGGAAA CCTGCAAGGA GCTCCTGCAG CTGTCCAAAC CAGAGCTTCC TCAAGATGGC 
ACCAGCACCC TCAGAGAAAA TAGCAAACCG GAAGAAAGCC ATTTGCTAGC CAAAAGGTAT 
GGGGGCTTCA TGAAAAGGTA TGGAGGCTTC ATGAAGAAAA TGGATGAGCT TTATCCCATG 
GAGCCAGAAG AAGAGGCCAA TGGAAGTGAG ATCCTCGCCA AGCGGTATGG GGGCTTCATG 
AAGAAGGATG CAGAGGAGGA CGACTCGCTG GCCAATTCCT CAGACCTGCT AAAAGAGCTT 
CTGGAAACAG GGGACAACCG AGAGCGTAGC CACCACCAGG ATGGCAGTGA TAATGAGGAA 
GAAGTGAGCA AGAGATATGG GGGCTTCATG AGAGGCTTAA AGAGAAGCCC CCAACTGGAA 
GATGAAGCCA AAGAGCTGCA GAAGCGATAT GGGGGCTTCA TGAGAAGAGT AGGTCGCCCA 
GAGTGGTGGA TGGACTACCA GAAACGGTAT GGAGGTTTCC TGAAGCGCTT TGCCGAGGCT 
CTGCCCTCCG ACGAAGAAGG CGAAAGTTAC TCCAAAGAAG TTCCTGAAAT GGAAAAAAGA 
TACGGAGGAT TTATGAGATT TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Elevation of Proenkephalin 143-183 in Cerebrospinal Fluid in Moyamoya Disease.
World neurosurgery109e446-e459(2018 Jan)
Yokoyama K,Maruwaka M,Yoshikawa K,Araki Y,Okamoto S,Sumitomo M,Kawamura A,Sakamoto Y,Shimizu K,Izumi T,Wakabayashi T


book

The effects of different exercise training modalities on plasma proenkephalin Peptide F in women.
Peptides9126-32(2017 May)
DuPont WH,Kraemer WJ,Nindl BC,Lee EC,Fragala MS,Hatfield DL,Caldwell LK,Post EM,Beeler MK,Volek JS,Maresh CM


book

Endogenous opioids contribute to insensitivity to pain in humans and mice lacking sodium channel Nav1.7.
Nature communications68967(2015 Dec)
Minett MS,Pereira V,Sikandar S,Matsuyama A,Lolignier S,Kanellopoulos AH,Mancini F,Iannetti GD,Bogdanov YD,Santana-Varela S,Millet Q,Baskozos G,MacAllister R,Cox JJ,Zhao J,Wood JN