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PFKM cDNA ORF clone, Homo sapiens(Human)

Gene Symbol PFKM
Entrez Gene ID 5213
Full Name phosphofructokinase, muscle
Synonyms ATP-PFK,GSD7,PFK-1,PFK-A,PFK1,PFKA,PFKX,PPP1R122
General protein information
Preferred Names

phosphofructokinase, muscle

Names

ATP-dependent 6-phosphofructokinase, muscle type
6-phosphofructo-1-kinase
6-phosphofructokinase type A
6-phosphofructokinase, muscle type
phosphofructo-1-kinase isozyme A
phosphofructokinase 1
phosphofructokinase, polypeptide X
phosphofructokinase-M
phosphohexokinase
protein phosphatase 1, regulatory subunit 122

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

12

12q13.11

Summary Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.[provided by RefSeq, Nov 2009].
Disorder MIM:

610681

Disorder Html: Glycogen storage disease VII, 232800 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_000289.5 NP_000280.1 ATP-dependent 6-phosphofructokinase, muscle type isoform 2
XM_005268976.3 XP_005269033.1 ATP-dependent 6-phosphofructokinase, muscle type isoform X1
XM_011538487.1 XP_011536789.1 ATP-dependent 6-phosphofructokinase, muscle type isoform X2
XM_005268977.1 XP_005269034.1 ATP-dependent 6-phosphofructokinase, muscle type isoform X3
NM_001166687.1 NP_001160159.1 ATP-dependent 6-phosphofructokinase, muscle type isoform 2
XM_017019469.1 XP_016874958.1 ATP-dependent 6-phosphofructokinase, muscle type isoform X4
XM_011538488.1 XP_011536790.1 ATP-dependent 6-phosphofructokinase, muscle type isoform X2
NM_001166686.1 NP_001160158.1 ATP-dependent 6-phosphofructokinase, muscle type isoform 1
XM_005268975.1 XP_005269032.1 ATP-dependent 6-phosphofructokinase, muscle type isoform X1
XM_005268978.2 XP_005269035.1 ATP-dependent 6-phosphofructokinase, muscle type isoform X3
XM_005268979.1 XP_005269036.1 ATP-dependent 6-phosphofructokinase, muscle type isoform X3
NM_001166688.1 NP_001160160.1 ATP-dependent 6-phosphofructokinase, muscle type isoform 2
XM_005268974.1 XP_005269031.1 ATP-dependent 6-phosphofructokinase, muscle type isoform X1
XM_017019468.1 XP_016874957.1 ATP-dependent 6-phosphofructokinase, muscle type isoform X3
XM_017019467.1 XP_016874956.1 ATP-dependent 6-phosphofructokinase, muscle type isoform X1
XM_011538488.2 XP_011536790.1 ATP-dependent 6-phosphofructokinase, muscle type isoform X5



Homo sapiens (human) PFKM NP_001160158.1
Rattus norvegicus (Norway rat) Pfkm NP_113903.1
Mus musculus (house mouse) Pfkm NP_067489.3
Xenopus tropicalis (tropical clawed frog) pfkm XP_004911968.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP007642 XP_564589.3
Pan troglodytes (chimpanzee) PFKM XP_509029.2
Canis lupus familiaris (dog) PFKM NP_001003199.1
Bos taurus (cattle) PFKM NP_001068736.1
Drosophila melanogaster (fruit fly) Pfk NP_724891.2
Caenorhabditis elegans (roundworm) Y71H10A.1 NP_741738.1
Macaca mulatta (rhesus monkey) PFKM XP_001096244.1
Gallus gallus (chicken) PFKM NP_989554.2
Danio rerio (zebrafish) pfkmb NP_001082886.1
Danio rerio (zebrafish) pfkma NP_001004575.1


Related articles in PubMed

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Yang X, Coulombe-Huntington J, Kang S, Sheynkman GM, Hao T, Richardson A, Sun S, Yang F, Shen YA, Murray RR, Spirohn K, Begg BE, Duran-Frigola M, MacWilliams A, Pevzner SJ, Zhong Q, Trigg SA, Tam S, Ghamsari L, Sahni N, Yi S, Rodriguez MD, Balcha D, Tan G, Costanzo M, Andrews B, Boone C, Zhou XJ, Salehi-Ashtiani K, Charloteaux B, Chen AA, Calderwood MA, Aloy P, Roth FP, Hill DE, Iakoucheva LM, Xia Y, Vidal M
Cell164(4)805-17(2016 Feb)

The expression pattern of PFKFB3 enzyme distinguishes between induced-pluripotent stem cells and cancer stem cells.
Cieślar-Pobuda A, Jain MV, Kratz G, Rzeszowska-Wolny J, Ghavami S, Wiechec E
Oncotarget6(30)29753-70(2015 Oct)

Structures of human phosphofructokinase-1 and atomic basis of cancer-associated mutations.
Webb BA, Forouhar F, Szu FE, Seetharaman J, Tong L, Barber DL
Nature523(7558)111-4(2015 Jul)

Novel testis- and embryo-specific isoforms of the phosphofructokinase-1 muscle type gene.
Yamada S, Nakajima H, Kuehn MR
Biochemical and biophysical research communications316(2)580-7(2004 Apr)

Physical and genetic mapping of the muscle phosphofructokinase gene (PFKM): reassignment to human chromosome 12q.
Howard TD, Akots G, Bowden DW
Genomics34(1)122-7(1996 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase3 (PFKFB3) and phosphofructokinase (PFK1) expression allows discrimination between induced pluripotent stem cells (iPS) and cancer stem cells (CSCs).
Title: The expression pattern of PFKFB3 enzyme distinguishes between induced-pluripotent stem cells and cancer stem cells.

characterize three PFK1 mutations and show they have distinct effects on allosteric regulation of PFKP activity and lactate production.
Title: Structures of human phosphofructokinase-1 and atomic basis of cancer-associated mutations.

Gene-based analysis identified an association between breast cancer and the phosphofructokinase-muscle (PFKM) gene on chromosome 12q13.11 that met the genome-wide gene-based threshold of 2.5 x 10(-6).
Title: A genome-wide association study of early-onset breast cancer identifies PFKM as a novel breast cancer gene and supports a common genetic spectrum for breast cancer at any age.

insulin/phosphofructokinase (PFKM), overlaps with an expression quantitative trait loci
Title: Expression of phosphofructokinase in skeletal muscle is influenced by genetic variation and associated with insulin sensitivity.

Asp(543)Ala, a naturally occurring Tarui disease-causing mutation in the activating binding site, causes an increased efficacy of ATP at the inhibitory allosteric binding site.
Title: Altered allosteric regulation of muscle 6-phosphofructokinase causes Tarui disease.

The following PFKM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PFKM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu28128 NM_000289.5
Latest version!
Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu28128 NM_001166688.1
Latest version!
Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu28128 NM_001166687.1
Latest version!
Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu28128 XM_011538488.2
Latest version!
Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X11, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu28127 NM_001166686.1
Latest version!
Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu28127 XM_005268979.1
Latest version!
Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu28127 XM_017019468.1
Latest version!
Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu28127 XM_005268978.2
Latest version!
Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu28127 XM_005268977.1
Latest version!
Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu46638 XM_005268974.1
Latest version!
Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu46638 XM_017019467.1
Latest version!
Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu46638 XM_005268975.1
Latest version!
Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu46638 XM_005268976.3
Latest version!
Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu59637 XM_011538487.1
Latest version!
Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu83887 XM_017019469.1
Latest version!
Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00
View Old Accession Versions >>
OHu28128 XM_011538488.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu28128
Clone ID Related Accession (Same CDS sequence) NM_000289.5 , NM_001166687.1 , XM_011538488.1 , NM_001166688.1 , XM_011538488.2
Accession Version NM_000289.5 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2343bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-08
Organism Homo sapiens(Human)
Product ATP-dependent 6-phosphofructokinase, muscle type isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA525437.1, BC021203.2, BC000534.2 and BU683561.1. On Nov 11, 2009 this sequence version replaced NM_000289.4. Transcript Variant: This variant (4, also known as type C) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Variants 2, 3 and 4 encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.88852.1, SRR1803612.128307.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

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ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000280.1
CDS186..2528
Misc Feature(1)189..1355
Misc Feature(2)189..191
Misc Feature(3)222..2507
Misc Feature(4)231..2450
Misc Feature(5)582..584
Misc Feature(6)675..683
Misc Feature(7)807..815
Misc Feature(8)1077..1088
Misc Feature(9)1314..1316
Misc Feature(10)1314..1316
Misc Feature(11)1356..1388
Misc Feature(12)1389..2525
Misc Feature(13)1767..1781
Misc Feature(14)1902..1910
Misc Feature(15)2166..2177
Misc Feature(16)2184..2186
Misc Feature(17)2508..2510
Misc Feature(18)2508..2510
Exon (1)1..177
Exon (2)178..270
Exon (3)271..344
Exon (4)345..422
Exon (5)423..612
Exon (6)613..778
Exon (7)779..823
Exon (8)824..932
Exon (9)933..1028
Exon (10)1029..1121
Exon (11)1122..1247
Exon (12)1248..1312
Exon (13)1313..1376
Exon (14)1377..1526
Exon (15)1527..1597
Exon (16)1598..1685
Exon (17)1686..1838
Exon (18)1839..2003
Exon (19)2004..2065
Exon (20)2066..2177
Exon (21)2178..2277
Exon (22)2278..2383
Exon (23)2384..3224
Translation

Target ORF information:

RefSeq Version NM_000289.5
Organism Homo sapiens(Human)
Definition Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000289.5

ORF Insert Sequence:

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ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28128
Clone ID Related Accession (Same CDS sequence) NM_000289.5 , NM_001166687.1 , XM_011538488.1 , NM_001166688.1 , XM_011538488.2
Accession Version NM_001166687.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2343bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-08
Organism Homo sapiens(Human)
Product ATP-dependent 6-phosphofructokinase, muscle type isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA560355.1, Y00698.1, BC000534.2 and BU683561.1. Transcript Variant: This variant (2, also known as type A) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Variants 2, 3 and 4 encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y00698.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2154361 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

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2161
2221
2281
2341
ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001160159.1
CDS40..2382
Misc Feature(1)28..30
Misc Feature(2)43..1209
Misc Feature(3)43..45
Misc Feature(4)76..2361
Misc Feature(5)85..2304
Misc Feature(6)436..438
Misc Feature(7)529..537
Misc Feature(8)661..669
Misc Feature(9)931..942
Misc Feature(10)1168..1170
Misc Feature(11)1210..1242
Misc Feature(12)1243..2379
Misc Feature(13)1621..1635
Misc Feature(14)1756..1764
Misc Feature(15)2020..2031
Misc Feature(16)2038..2040
Misc Feature(17)2362..2364
Exon (1)1..31
Exon (2)32..124
Exon (3)125..198
Exon (4)199..276
Exon (5)277..466
Exon (6)467..632
Exon (7)633..677
Exon (8)678..786
Exon (9)787..882
Exon (10)883..975
Exon (11)976..1101
Exon (12)1102..1166
Exon (13)1167..1230
Exon (14)1231..1380
Exon (15)1381..1451
Exon (16)1452..1539
Exon (17)1540..1692
Exon (18)1693..1857
Exon (19)1858..1919
Exon (20)1920..2031
Exon (21)2032..2131
Exon (22)2132..2237
Exon (23)2238..3078
Translation

Target ORF information:

RefSeq Version NM_001166687.1
Organism Homo sapiens(Human)
Definition Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001166687.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28128
Clone ID Related Accession (Same CDS sequence) NM_000289.5 , NM_001166687.1 , XM_011538488.1 , NM_001166688.1 , XM_011538488.2
Accession Version NM_001166688.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2343bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-08
Organism Homo sapiens(Human)
Product ATP-dependent 6-phosphofructokinase, muscle type isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA761651.1, BC000534.2 and BU683561.1. Transcript Variant: This variant (3, also known as type B) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Variants 2, 3 and 4 encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.91353.1, BC000534.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001160160.1
CDS129..2471
Misc Feature(1)111..113
Misc Feature(2)132..1298
Misc Feature(3)132..134
Misc Feature(4)165..2450
Misc Feature(5)174..2393
Misc Feature(6)525..527
Misc Feature(7)618..626
Misc Feature(8)750..758
Misc Feature(9)1020..1031
Misc Feature(10)1257..1259
Misc Feature(11)1299..1331
Misc Feature(12)1332..2468
Misc Feature(13)1710..1724
Misc Feature(14)1845..1853
Misc Feature(15)2109..2120
Misc Feature(16)2127..2129
Misc Feature(17)2451..2453
Exon (1)1..213
Exon (2)214..287
Exon (3)288..365
Exon (4)366..555
Exon (5)556..721
Exon (6)722..766
Exon (7)767..875
Exon (8)876..971
Exon (9)972..1064
Exon (10)1065..1190
Exon (11)1191..1255
Exon (12)1256..1319
Exon (13)1320..1469
Exon (14)1470..1540
Exon (15)1541..1628
Exon (16)1629..1781
Exon (17)1782..1946
Exon (18)1947..2008
Exon (19)2009..2120
Exon (20)2121..2220
Exon (21)2221..2326
Exon (22)2327..3167
Translation

Target ORF information:

RefSeq Version NM_001166688.1
Organism Homo sapiens(Human)
Definition Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001166688.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28128
Clone ID Related Accession (Same CDS sequence) NM_000289.5 , NM_001166687.1 , XM_011538488.1 , NM_001166688.1 , XM_011538488.2
Accession Version XM_011538488.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2343bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-03-12
Organism Homo sapiens(Human)
Product ATP-dependent 6-phosphofructokinase, muscle type isoform X2
Comment

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011536790.1
Translation

Target ORF information:

RefSeq Version XM_011538488.1
Organism Homo sapiens(Human)
Definition Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011538488.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28128
Clone ID Related Accession (Same CDS sequence) NM_000289.5 , NM_001166687.1 , XM_011538488.1 , NM_001166688.1 , XM_011538488.2
Accession Version XM_011538488.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2343bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product ATP-dependent 6-phosphofructokinase, muscle type isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011538488.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011536790.1
CDS151..2493
Misc Feature(1)187..2472
Misc Feature(2)196..2415
Translation

Target ORF information:

RefSeq Version XM_011538488.2
Organism Homo sapiens(Human)
Definition Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X11, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011538488.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28127
Clone ID Related Accession (Same CDS sequence) XM_005268977.1 , NM_001166686.1 , XM_005268978.2 , XM_005268979.1 , XM_017019468.1
Accession Version NM_001166686.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2556bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-08
Organism Homo sapiens(Human)
Product ATP-dependent 6-phosphofructokinase, muscle type isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB081387.1, BC021203.2, BC000534.2 and BU683561.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) represents the longest transcript (also known as TE-PFKM) and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK126229.1, SRR1803613.90467.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001160158.1
CDS275..2830
Misc Feature(1)263..265
Misc Feature(2)524..2809
Misc Feature(3)533..2752
Exon (1)1..265
Exon (2)266..356
Exon (3)357..479
Exon (4)480..572
Exon (5)573..646
Exon (6)647..724
Exon (7)725..914
Exon (8)915..1080
Exon (9)1081..1125
Exon (10)1126..1234
Exon (11)1235..1330
Exon (12)1331..1423
Exon (13)1424..1549
Exon (14)1550..1614
Exon (15)1615..1678
Exon (16)1679..1828
Exon (17)1829..1899
Exon (18)1900..1987
Exon (19)1988..2140
Exon (20)2141..2305
Exon (21)2306..2367
Exon (22)2368..2479
Exon (23)2480..2579
Exon (24)2580..2685
Exon (25)2686..3526
Translation

Target ORF information:

RefSeq Version NM_001166686.1
Organism Homo sapiens(Human)
Definition Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001166686.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28127
Clone ID Related Accession (Same CDS sequence) XM_005268977.1 , NM_001166686.1 , XM_005268978.2 , XM_005268979.1 , XM_017019468.1
Accession Version XM_005268977.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2556bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product ATP-dependent 6-phosphofructokinase, muscle type isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005269034.1
CDS158..2713
Misc Feature(1)407..2692
Misc Feature(2)416..2635
Translation

Target ORF information:

RefSeq Version XM_005268977.1
Organism Homo sapiens(Human)
Definition Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005268977.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28127
Clone ID Related Accession (Same CDS sequence) XM_005268977.1 , NM_001166686.1 , XM_005268978.2 , XM_005268979.1 , XM_017019468.1
Accession Version XM_005268978.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2556bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product ATP-dependent 6-phosphofructokinase, muscle type isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 3, 2014 this sequence version replaced XM_005268978.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005269035.1
CDS71..2626
Misc Feature(1)320..2605
Misc Feature(2)329..2548
Translation

Target ORF information:

RefSeq Version XM_005268978.2
Organism Homo sapiens(Human)
Definition Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005268978.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28127
Clone ID Related Accession (Same CDS sequence) XM_005268977.1 , NM_001166686.1 , XM_005268978.2 , XM_005268979.1 , XM_017019468.1
Accession Version XM_005268979.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2556bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product ATP-dependent 6-phosphofructokinase, muscle type isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005269036.1
CDS158..2713
Misc Feature(1)407..2692
Misc Feature(2)416..2635
Translation

Target ORF information:

RefSeq Version XM_005268979.1
Organism Homo sapiens(Human)
Definition Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005268979.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28127
Clone ID Related Accession (Same CDS sequence) XM_005268977.1 , NM_001166686.1 , XM_005268978.2 , XM_005268979.1 , XM_017019468.1
Accession Version XM_017019468.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2556bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product ATP-dependent 6-phosphofructokinase, muscle type isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016874957.1
CDS93..2648
Misc Feature(1)342..2627
Misc Feature(2)351..2570
Translation

Target ORF information:

RefSeq Version XM_017019468.1
Organism Homo sapiens(Human)
Definition Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017019468.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu46638
Clone ID Related Accession (Same CDS sequence) XM_005268976.3 , XM_005268975.1 , XM_005268974.1 , XM_017019467.1
Accession Version XM_005268976.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2652bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product ATP-dependent 6-phosphofructokinase, muscle type isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005268976.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAATGT GCAGAGGTAG AGATACAAGT AAGCAAGAGG
AAGAGGGCAG TGGTAAAAGC AAGAGGAGAC CCGACTGTGG AGACAATGAA GCAAAGGGAA
GAGTGGATCA TGACCCATGA AGAGCACCAT GCAGCCAAAA CCCTGGGGAT TGGCAAAGCC
ATTGCTGTCT TAACCTCTGG TGGAGATGCC CAAGGTATGA ATGCTGCTGT CAGGGCTGTG
GTTCGAGTTG GTATCTTCAC CGGTGCCCGT GTCTTCTTTG TCCATGAGGG TTATCAAGGC
CTGGTGGATG GTGGAGATCA CATCAAGGAA GCCACCTGGG AGAGCGTTTC GATGATGCTT
CAGCTGGGAG GCACGGTGAT TGGAAGTGCC CGGTGCAAGG ACTTTCGGGA ACGAGAAGGA
CGACTCCGAG CTGCCTACAA CCTGGTGAAG CGTGGGATCA CCAATCTCTG TGTCATTGGG
GGTGATGGCA GCCTCACTGG GGCTGACACC TTCCGTTCTG AGTGGAGTGA CTTGTTGAGT
GACCTCCAGA AAGCAGGTAA GATCACAGAT GAGGAGGCTA CGAAGTCCAG CTACCTGAAC
ATTGTGGGCC TGGTTGGGTC AATTGACAAT GACTTCTGTG GCACCGATAT GACCATTGGC
ACTGACTCTG CCCTGCATCG GATCATGGAA ATTGTAGATG CCATCACTAC CACTGCCCAG
AGCCACCAGA GGACATTTGT GTTAGAAGTA ATGGGCCGCC ACTGTGGATA CCTGGCCCTT
GTCACCTCTC TGTCCTGTGG GGCCGACTGG GTTTTTATTC CTGAATGTCC ACCAGATGAC
GACTGGGAGG AACACCTTTG TCGCCGACTC AGCGAGACAA GGACCCGTGG TTCTCGTCTC
AACATCATCA TTGTGGCTGA GGGTGCAATT GACAAGAATG GAAAACCAAT CACCTCAGAA
GACATCAAGA ATCTGGTGGT TAAGCGTCTG GGATATGACA CCCGGGTTAC TGTCTTGGGG
CATGTGCAGA GGGGTGGGAC GCCATCAGCC TTTGACAGAA TTCTGGGCAG CAGGATGGGT
GTGGAAGCAG TGATGGCACT TTTGGAGGGG ACCCCAGATA CCCCAGCCTG TGTAGTGAGC
CTCTCTGGTA ACCAGGCTGT GCGCCTGCCC CTCATGGAAT GTGTCCAGGT GACCAAAGAT
GTGACCAAGG CCATGGATGA GAAGAAATTT GACGAAGCCC TGAAGCTGAG AGGCCGGAGC
TTCATGAACA ACTGGGAGGT GTACAAGCTT CTAGCTCATG TCAGACCCCC GGTATCTAAG
AGTGGTTCGC ACACAGTGGC TGTGATGAAC GTGGGGGCTC CGGCTGCAGG CATGAATGCT
GCTGTTCGCT CCACTGTGAG GATTGGCCTT ATCCAGGGCA ACCGAGTGCT CGTTGTCCAT
GATGGTTTCG AGGGCCTGGC CAAGGGGCAG ATAGAGGAAG CTGGCTGGAG CTATGTTGGG
GGCTGGACTG GCCAAGGTGG CTCTAAACTT GGGACTAAAA GGACTCTACC CAAGAAGAGC
TTTGAACAGA TCAGTGCCAA TATAACTAAG TTTAACATTC AGGGCCTTGT CATCATTGGG
GGCTTTGAGG CTTACACAGG GGGCCTGGAA CTGATGGAGG GCAGGAAGCA GTTTGATGAG
CTCTGCATCC CATTTGTGGT CATTCCTGCT ACAGTCTCCA ACAATGTCCC TGGCTCAGAC
TTCAGCGTTG GGGCTGACAC AGCACTCAAT ACTATCTGCA CAACCTGTGA CCGCATCAAG
CAGTCAGCAG CTGGCACCAA GCGTCGGGTG TTTATCATTG AGACTATGGG TGGCTACTGT
GGCTACCTGG CTACCATGGC TGGACTGGCA GCTGGGGCCG ATGCTGCCTA CATTTTTGAG
GAGCCCTTCA CCATTCGAGA CCTGCAGGCA AATGTTGAAC ATCTGGTGCA AAAGATGAAA
ACAACTGTGA AAAGGGGCTT GGTGTTAAGG AATGAAAAGT GCAATGAGAA CTATACCACT
GACTTCATTT TCAACCTGTA CTCTGAGGAG GGGAAGGGCA TCTTCGACAG CAGGAAGAAT
GTGCTTGGTC ACATGCAGCA GGGTGGGAGC CCAACCCCAT TTGATAGGAA TTTTGCCACT
AAGATGGGCG CCAAGGCTAT GAACTGGATG TCTGGGAAAA TCAAAGAGAG TTACCGTAAT
GGGCGGATCT TTGCCAATAC TCCAGATTCG GGCTGTGTTC TGGGGATGCG TAAGAGGGCT
CTGGTCTTCC AACCAGTGGC TGAGCTGAAG GACCAGACAG ATTTTGAGCA TCGAATCCCC
AAGGAACAGT GGTGGCTGAA ACTGAGGCCC ATCCTCAAAA TCCTAGCCAA GTACGAGATT
GACTTGGACA CTTCAGACCA TGCCCACCTG GAGCACATCA CCCGGAAGCG GTCCGGGGAA
GCTGCCGTCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005269033.1
CDS258..2909
Misc Feature(1)603..2888
Misc Feature(2)612..2831
Translation

Target ORF information:

RefSeq Version XM_005268976.3
Organism Homo sapiens(Human)
Definition Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005268976.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAATGT GCAGAGGTAG AGATACAAGT AAGCAAGAGG
AAGAGGGCAG TGGTAAAAGC AAGAGGAGAC CCGACTGTGG AGACAATGAA GCAAAGGGAA
GAGTGGATCA TGACCCATGA AGAGCACCAT GCAGCCAAAA CCCTGGGGAT TGGCAAAGCC
ATTGCTGTCT TAACCTCTGG TGGAGATGCC CAAGGTATGA ATGCTGCTGT CAGGGCTGTG
GTTCGAGTTG GTATCTTCAC CGGTGCCCGT GTCTTCTTTG TCCATGAGGG TTATCAAGGC
CTGGTGGATG GTGGAGATCA CATCAAGGAA GCCACCTGGG AGAGCGTTTC GATGATGCTT
CAGCTGGGAG GCACGGTGAT TGGAAGTGCC CGGTGCAAGG ACTTTCGGGA ACGAGAAGGA
CGACTCCGAG CTGCCTACAA CCTGGTGAAG CGTGGGATCA CCAATCTCTG TGTCATTGGG
GGTGATGGCA GCCTCACTGG GGCTGACACC TTCCGTTCTG AGTGGAGTGA CTTGTTGAGT
GACCTCCAGA AAGCAGGTAA GATCACAGAT GAGGAGGCTA CGAAGTCCAG CTACCTGAAC
ATTGTGGGCC TGGTTGGGTC AATTGACAAT GACTTCTGTG GCACCGATAT GACCATTGGC
ACTGACTCTG CCCTGCATCG GATCATGGAA ATTGTAGATG CCATCACTAC CACTGCCCAG
AGCCACCAGA GGACATTTGT GTTAGAAGTA ATGGGCCGCC ACTGTGGATA CCTGGCCCTT
GTCACCTCTC TGTCCTGTGG GGCCGACTGG GTTTTTATTC CTGAATGTCC ACCAGATGAC
GACTGGGAGG AACACCTTTG TCGCCGACTC AGCGAGACAA GGACCCGTGG TTCTCGTCTC
AACATCATCA TTGTGGCTGA GGGTGCAATT GACAAGAATG GAAAACCAAT CACCTCAGAA
GACATCAAGA ATCTGGTGGT TAAGCGTCTG GGATATGACA CCCGGGTTAC TGTCTTGGGG
CATGTGCAGA GGGGTGGGAC GCCATCAGCC TTTGACAGAA TTCTGGGCAG CAGGATGGGT
GTGGAAGCAG TGATGGCACT TTTGGAGGGG ACCCCAGATA CCCCAGCCTG TGTAGTGAGC
CTCTCTGGTA ACCAGGCTGT GCGCCTGCCC CTCATGGAAT GTGTCCAGGT GACCAAAGAT
GTGACCAAGG CCATGGATGA GAAGAAATTT GACGAAGCCC TGAAGCTGAG AGGCCGGAGC
TTCATGAACA ACTGGGAGGT GTACAAGCTT CTAGCTCATG TCAGACCCCC GGTATCTAAG
AGTGGTTCGC ACACAGTGGC TGTGATGAAC GTGGGGGCTC CGGCTGCAGG CATGAATGCT
GCTGTTCGCT CCACTGTGAG GATTGGCCTT ATCCAGGGCA ACCGAGTGCT CGTTGTCCAT
GATGGTTTCG AGGGCCTGGC CAAGGGGCAG ATAGAGGAAG CTGGCTGGAG CTATGTTGGG
GGCTGGACTG GCCAAGGTGG CTCTAAACTT GGGACTAAAA GGACTCTACC CAAGAAGAGC
TTTGAACAGA TCAGTGCCAA TATAACTAAG TTTAACATTC AGGGCCTTGT CATCATTGGG
GGCTTTGAGG CTTACACAGG GGGCCTGGAA CTGATGGAGG GCAGGAAGCA GTTTGATGAG
CTCTGCATCC CATTTGTGGT CATTCCTGCT ACAGTCTCCA ACAATGTCCC TGGCTCAGAC
TTCAGCGTTG GGGCTGACAC AGCACTCAAT ACTATCTGCA CAACCTGTGA CCGCATCAAG
CAGTCAGCAG CTGGCACCAA GCGTCGGGTG TTTATCATTG AGACTATGGG TGGCTACTGT
GGCTACCTGG CTACCATGGC TGGACTGGCA GCTGGGGCCG ATGCTGCCTA CATTTTTGAG
GAGCCCTTCA CCATTCGAGA CCTGCAGGCA AATGTTGAAC ATCTGGTGCA AAAGATGAAA
ACAACTGTGA AAAGGGGCTT GGTGTTAAGG AATGAAAAGT GCAATGAGAA CTATACCACT
GACTTCATTT TCAACCTGTA CTCTGAGGAG GGGAAGGGCA TCTTCGACAG CAGGAAGAAT
GTGCTTGGTC ACATGCAGCA GGGTGGGAGC CCAACCCCAT TTGATAGGAA TTTTGCCACT
AAGATGGGCG CCAAGGCTAT GAACTGGATG TCTGGGAAAA TCAAAGAGAG TTACCGTAAT
GGGCGGATCT TTGCCAATAC TCCAGATTCG GGCTGTGTTC TGGGGATGCG TAAGAGGGCT
CTGGTCTTCC AACCAGTGGC TGAGCTGAAG GACCAGACAG ATTTTGAGCA TCGAATCCCC
AAGGAACAGT GGTGGCTGAA ACTGAGGCCC ATCCTCAAAA TCCTAGCCAA GTACGAGATT
GACTTGGACA CTTCAGACCA TGCCCACCTG GAGCACATCA CCCGGAAGCG GTCCGGGGAA
GCTGCCGTCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu46638
Clone ID Related Accession (Same CDS sequence) XM_005268976.3 , XM_005268975.1 , XM_005268974.1 , XM_017019467.1
Accession Version XM_005268975.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2652bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product ATP-dependent 6-phosphofructokinase, muscle type isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAATGT GCAGAGGTAG AGATACAAGT AAGCAAGAGG
AAGAGGGCAG TGGTAAAAGC AAGAGGAGAC CCGACTGTGG AGACAATGAA GCAAAGGGAA
GAGTGGATCA TGACCCATGA AGAGCACCAT GCAGCCAAAA CCCTGGGGAT TGGCAAAGCC
ATTGCTGTCT TAACCTCTGG TGGAGATGCC CAAGGTATGA ATGCTGCTGT CAGGGCTGTG
GTTCGAGTTG GTATCTTCAC CGGTGCCCGT GTCTTCTTTG TCCATGAGGG TTATCAAGGC
CTGGTGGATG GTGGAGATCA CATCAAGGAA GCCACCTGGG AGAGCGTTTC GATGATGCTT
CAGCTGGGAG GCACGGTGAT TGGAAGTGCC CGGTGCAAGG ACTTTCGGGA ACGAGAAGGA
CGACTCCGAG CTGCCTACAA CCTGGTGAAG CGTGGGATCA CCAATCTCTG TGTCATTGGG
GGTGATGGCA GCCTCACTGG GGCTGACACC TTCCGTTCTG AGTGGAGTGA CTTGTTGAGT
GACCTCCAGA AAGCAGGTAA GATCACAGAT GAGGAGGCTA CGAAGTCCAG CTACCTGAAC
ATTGTGGGCC TGGTTGGGTC AATTGACAAT GACTTCTGTG GCACCGATAT GACCATTGGC
ACTGACTCTG CCCTGCATCG GATCATGGAA ATTGTAGATG CCATCACTAC CACTGCCCAG
AGCCACCAGA GGACATTTGT GTTAGAAGTA ATGGGCCGCC ACTGTGGATA CCTGGCCCTT
GTCACCTCTC TGTCCTGTGG GGCCGACTGG GTTTTTATTC CTGAATGTCC ACCAGATGAC
GACTGGGAGG AACACCTTTG TCGCCGACTC AGCGAGACAA GGACCCGTGG TTCTCGTCTC
AACATCATCA TTGTGGCTGA GGGTGCAATT GACAAGAATG GAAAACCAAT CACCTCAGAA
GACATCAAGA ATCTGGTGGT TAAGCGTCTG GGATATGACA CCCGGGTTAC TGTCTTGGGG
CATGTGCAGA GGGGTGGGAC GCCATCAGCC TTTGACAGAA TTCTGGGCAG CAGGATGGGT
GTGGAAGCAG TGATGGCACT TTTGGAGGGG ACCCCAGATA CCCCAGCCTG TGTAGTGAGC
CTCTCTGGTA ACCAGGCTGT GCGCCTGCCC CTCATGGAAT GTGTCCAGGT GACCAAAGAT
GTGACCAAGG CCATGGATGA GAAGAAATTT GACGAAGCCC TGAAGCTGAG AGGCCGGAGC
TTCATGAACA ACTGGGAGGT GTACAAGCTT CTAGCTCATG TCAGACCCCC GGTATCTAAG
AGTGGTTCGC ACACAGTGGC TGTGATGAAC GTGGGGGCTC CGGCTGCAGG CATGAATGCT
GCTGTTCGCT CCACTGTGAG GATTGGCCTT ATCCAGGGCA ACCGAGTGCT CGTTGTCCAT
GATGGTTTCG AGGGCCTGGC CAAGGGGCAG ATAGAGGAAG CTGGCTGGAG CTATGTTGGG
GGCTGGACTG GCCAAGGTGG CTCTAAACTT GGGACTAAAA GGACTCTACC CAAGAAGAGC
TTTGAACAGA TCAGTGCCAA TATAACTAAG TTTAACATTC AGGGCCTTGT CATCATTGGG
GGCTTTGAGG CTTACACAGG GGGCCTGGAA CTGATGGAGG GCAGGAAGCA GTTTGATGAG
CTCTGCATCC CATTTGTGGT CATTCCTGCT ACAGTCTCCA ACAATGTCCC TGGCTCAGAC
TTCAGCGTTG GGGCTGACAC AGCACTCAAT ACTATCTGCA CAACCTGTGA CCGCATCAAG
CAGTCAGCAG CTGGCACCAA GCGTCGGGTG TTTATCATTG AGACTATGGG TGGCTACTGT
GGCTACCTGG CTACCATGGC TGGACTGGCA GCTGGGGCCG ATGCTGCCTA CATTTTTGAG
GAGCCCTTCA CCATTCGAGA CCTGCAGGCA AATGTTGAAC ATCTGGTGCA AAAGATGAAA
ACAACTGTGA AAAGGGGCTT GGTGTTAAGG AATGAAAAGT GCAATGAGAA CTATACCACT
GACTTCATTT TCAACCTGTA CTCTGAGGAG GGGAAGGGCA TCTTCGACAG CAGGAAGAAT
GTGCTTGGTC ACATGCAGCA GGGTGGGAGC CCAACCCCAT TTGATAGGAA TTTTGCCACT
AAGATGGGCG CCAAGGCTAT GAACTGGATG TCTGGGAAAA TCAAAGAGAG TTACCGTAAT
GGGCGGATCT TTGCCAATAC TCCAGATTCG GGCTGTGTTC TGGGGATGCG TAAGAGGGCT
CTGGTCTTCC AACCAGTGGC TGAGCTGAAG GACCAGACAG ATTTTGAGCA TCGAATCCCC
AAGGAACAGT GGTGGCTGAA ACTGAGGCCC ATCCTCAAAA TCCTAGCCAA GTACGAGATT
GACTTGGACA CTTCAGACCA TGCCCACCTG GAGCACATCA CCCGGAAGCG GTCCGGGGAA
GCTGCCGTCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005269032.1
CDS215..2866
Misc Feature(1)560..2845
Misc Feature(2)569..2788
Translation

Target ORF information:

RefSeq Version XM_005268975.1
Organism Homo sapiens(Human)
Definition Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005268975.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAATGT GCAGAGGTAG AGATACAAGT AAGCAAGAGG
AAGAGGGCAG TGGTAAAAGC AAGAGGAGAC CCGACTGTGG AGACAATGAA GCAAAGGGAA
GAGTGGATCA TGACCCATGA AGAGCACCAT GCAGCCAAAA CCCTGGGGAT TGGCAAAGCC
ATTGCTGTCT TAACCTCTGG TGGAGATGCC CAAGGTATGA ATGCTGCTGT CAGGGCTGTG
GTTCGAGTTG GTATCTTCAC CGGTGCCCGT GTCTTCTTTG TCCATGAGGG TTATCAAGGC
CTGGTGGATG GTGGAGATCA CATCAAGGAA GCCACCTGGG AGAGCGTTTC GATGATGCTT
CAGCTGGGAG GCACGGTGAT TGGAAGTGCC CGGTGCAAGG ACTTTCGGGA ACGAGAAGGA
CGACTCCGAG CTGCCTACAA CCTGGTGAAG CGTGGGATCA CCAATCTCTG TGTCATTGGG
GGTGATGGCA GCCTCACTGG GGCTGACACC TTCCGTTCTG AGTGGAGTGA CTTGTTGAGT
GACCTCCAGA AAGCAGGTAA GATCACAGAT GAGGAGGCTA CGAAGTCCAG CTACCTGAAC
ATTGTGGGCC TGGTTGGGTC AATTGACAAT GACTTCTGTG GCACCGATAT GACCATTGGC
ACTGACTCTG CCCTGCATCG GATCATGGAA ATTGTAGATG CCATCACTAC CACTGCCCAG
AGCCACCAGA GGACATTTGT GTTAGAAGTA ATGGGCCGCC ACTGTGGATA CCTGGCCCTT
GTCACCTCTC TGTCCTGTGG GGCCGACTGG GTTTTTATTC CTGAATGTCC ACCAGATGAC
GACTGGGAGG AACACCTTTG TCGCCGACTC AGCGAGACAA GGACCCGTGG TTCTCGTCTC
AACATCATCA TTGTGGCTGA GGGTGCAATT GACAAGAATG GAAAACCAAT CACCTCAGAA
GACATCAAGA ATCTGGTGGT TAAGCGTCTG GGATATGACA CCCGGGTTAC TGTCTTGGGG
CATGTGCAGA GGGGTGGGAC GCCATCAGCC TTTGACAGAA TTCTGGGCAG CAGGATGGGT
GTGGAAGCAG TGATGGCACT TTTGGAGGGG ACCCCAGATA CCCCAGCCTG TGTAGTGAGC
CTCTCTGGTA ACCAGGCTGT GCGCCTGCCC CTCATGGAAT GTGTCCAGGT GACCAAAGAT
GTGACCAAGG CCATGGATGA GAAGAAATTT GACGAAGCCC TGAAGCTGAG AGGCCGGAGC
TTCATGAACA ACTGGGAGGT GTACAAGCTT CTAGCTCATG TCAGACCCCC GGTATCTAAG
AGTGGTTCGC ACACAGTGGC TGTGATGAAC GTGGGGGCTC CGGCTGCAGG CATGAATGCT
GCTGTTCGCT CCACTGTGAG GATTGGCCTT ATCCAGGGCA ACCGAGTGCT CGTTGTCCAT
GATGGTTTCG AGGGCCTGGC CAAGGGGCAG ATAGAGGAAG CTGGCTGGAG CTATGTTGGG
GGCTGGACTG GCCAAGGTGG CTCTAAACTT GGGACTAAAA GGACTCTACC CAAGAAGAGC
TTTGAACAGA TCAGTGCCAA TATAACTAAG TTTAACATTC AGGGCCTTGT CATCATTGGG
GGCTTTGAGG CTTACACAGG GGGCCTGGAA CTGATGGAGG GCAGGAAGCA GTTTGATGAG
CTCTGCATCC CATTTGTGGT CATTCCTGCT ACAGTCTCCA ACAATGTCCC TGGCTCAGAC
TTCAGCGTTG GGGCTGACAC AGCACTCAAT ACTATCTGCA CAACCTGTGA CCGCATCAAG
CAGTCAGCAG CTGGCACCAA GCGTCGGGTG TTTATCATTG AGACTATGGG TGGCTACTGT
GGCTACCTGG CTACCATGGC TGGACTGGCA GCTGGGGCCG ATGCTGCCTA CATTTTTGAG
GAGCCCTTCA CCATTCGAGA CCTGCAGGCA AATGTTGAAC ATCTGGTGCA AAAGATGAAA
ACAACTGTGA AAAGGGGCTT GGTGTTAAGG AATGAAAAGT GCAATGAGAA CTATACCACT
GACTTCATTT TCAACCTGTA CTCTGAGGAG GGGAAGGGCA TCTTCGACAG CAGGAAGAAT
GTGCTTGGTC ACATGCAGCA GGGTGGGAGC CCAACCCCAT TTGATAGGAA TTTTGCCACT
AAGATGGGCG CCAAGGCTAT GAACTGGATG TCTGGGAAAA TCAAAGAGAG TTACCGTAAT
GGGCGGATCT TTGCCAATAC TCCAGATTCG GGCTGTGTTC TGGGGATGCG TAAGAGGGCT
CTGGTCTTCC AACCAGTGGC TGAGCTGAAG GACCAGACAG ATTTTGAGCA TCGAATCCCC
AAGGAACAGT GGTGGCTGAA ACTGAGGCCC ATCCTCAAAA TCCTAGCCAA GTACGAGATT
GACTTGGACA CTTCAGACCA TGCCCACCTG GAGCACATCA CCCGGAAGCG GTCCGGGGAA
GCTGCCGTCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu46638
Clone ID Related Accession (Same CDS sequence) XM_005268976.3 , XM_005268975.1 , XM_005268974.1 , XM_017019467.1
Accession Version XM_005268974.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2652bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product ATP-dependent 6-phosphofructokinase, muscle type isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAATGT GCAGAGGTAG AGATACAAGT AAGCAAGAGG
AAGAGGGCAG TGGTAAAAGC AAGAGGAGAC CCGACTGTGG AGACAATGAA GCAAAGGGAA
GAGTGGATCA TGACCCATGA AGAGCACCAT GCAGCCAAAA CCCTGGGGAT TGGCAAAGCC
ATTGCTGTCT TAACCTCTGG TGGAGATGCC CAAGGTATGA ATGCTGCTGT CAGGGCTGTG
GTTCGAGTTG GTATCTTCAC CGGTGCCCGT GTCTTCTTTG TCCATGAGGG TTATCAAGGC
CTGGTGGATG GTGGAGATCA CATCAAGGAA GCCACCTGGG AGAGCGTTTC GATGATGCTT
CAGCTGGGAG GCACGGTGAT TGGAAGTGCC CGGTGCAAGG ACTTTCGGGA ACGAGAAGGA
CGACTCCGAG CTGCCTACAA CCTGGTGAAG CGTGGGATCA CCAATCTCTG TGTCATTGGG
GGTGATGGCA GCCTCACTGG GGCTGACACC TTCCGTTCTG AGTGGAGTGA CTTGTTGAGT
GACCTCCAGA AAGCAGGTAA GATCACAGAT GAGGAGGCTA CGAAGTCCAG CTACCTGAAC
ATTGTGGGCC TGGTTGGGTC AATTGACAAT GACTTCTGTG GCACCGATAT GACCATTGGC
ACTGACTCTG CCCTGCATCG GATCATGGAA ATTGTAGATG CCATCACTAC CACTGCCCAG
AGCCACCAGA GGACATTTGT GTTAGAAGTA ATGGGCCGCC ACTGTGGATA CCTGGCCCTT
GTCACCTCTC TGTCCTGTGG GGCCGACTGG GTTTTTATTC CTGAATGTCC ACCAGATGAC
GACTGGGAGG AACACCTTTG TCGCCGACTC AGCGAGACAA GGACCCGTGG TTCTCGTCTC
AACATCATCA TTGTGGCTGA GGGTGCAATT GACAAGAATG GAAAACCAAT CACCTCAGAA
GACATCAAGA ATCTGGTGGT TAAGCGTCTG GGATATGACA CCCGGGTTAC TGTCTTGGGG
CATGTGCAGA GGGGTGGGAC GCCATCAGCC TTTGACAGAA TTCTGGGCAG CAGGATGGGT
GTGGAAGCAG TGATGGCACT TTTGGAGGGG ACCCCAGATA CCCCAGCCTG TGTAGTGAGC
CTCTCTGGTA ACCAGGCTGT GCGCCTGCCC CTCATGGAAT GTGTCCAGGT GACCAAAGAT
GTGACCAAGG CCATGGATGA GAAGAAATTT GACGAAGCCC TGAAGCTGAG AGGCCGGAGC
TTCATGAACA ACTGGGAGGT GTACAAGCTT CTAGCTCATG TCAGACCCCC GGTATCTAAG
AGTGGTTCGC ACACAGTGGC TGTGATGAAC GTGGGGGCTC CGGCTGCAGG CATGAATGCT
GCTGTTCGCT CCACTGTGAG GATTGGCCTT ATCCAGGGCA ACCGAGTGCT CGTTGTCCAT
GATGGTTTCG AGGGCCTGGC CAAGGGGCAG ATAGAGGAAG CTGGCTGGAG CTATGTTGGG
GGCTGGACTG GCCAAGGTGG CTCTAAACTT GGGACTAAAA GGACTCTACC CAAGAAGAGC
TTTGAACAGA TCAGTGCCAA TATAACTAAG TTTAACATTC AGGGCCTTGT CATCATTGGG
GGCTTTGAGG CTTACACAGG GGGCCTGGAA CTGATGGAGG GCAGGAAGCA GTTTGATGAG
CTCTGCATCC CATTTGTGGT CATTCCTGCT ACAGTCTCCA ACAATGTCCC TGGCTCAGAC
TTCAGCGTTG GGGCTGACAC AGCACTCAAT ACTATCTGCA CAACCTGTGA CCGCATCAAG
CAGTCAGCAG CTGGCACCAA GCGTCGGGTG TTTATCATTG AGACTATGGG TGGCTACTGT
GGCTACCTGG CTACCATGGC TGGACTGGCA GCTGGGGCCG ATGCTGCCTA CATTTTTGAG
GAGCCCTTCA CCATTCGAGA CCTGCAGGCA AATGTTGAAC ATCTGGTGCA AAAGATGAAA
ACAACTGTGA AAAGGGGCTT GGTGTTAAGG AATGAAAAGT GCAATGAGAA CTATACCACT
GACTTCATTT TCAACCTGTA CTCTGAGGAG GGGAAGGGCA TCTTCGACAG CAGGAAGAAT
GTGCTTGGTC ACATGCAGCA GGGTGGGAGC CCAACCCCAT TTGATAGGAA TTTTGCCACT
AAGATGGGCG CCAAGGCTAT GAACTGGATG TCTGGGAAAA TCAAAGAGAG TTACCGTAAT
GGGCGGATCT TTGCCAATAC TCCAGATTCG GGCTGTGTTC TGGGGATGCG TAAGAGGGCT
CTGGTCTTCC AACCAGTGGC TGAGCTGAAG GACCAGACAG ATTTTGAGCA TCGAATCCCC
AAGGAACAGT GGTGGCTGAA ACTGAGGCCC ATCCTCAAAA TCCTAGCCAA GTACGAGATT
GACTTGGACA CTTCAGACCA TGCCCACCTG GAGCACATCA CCCGGAAGCG GTCCGGGGAA
GCTGCCGTCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005269031.1
CDS76..2727
Misc Feature(1)421..2706
Misc Feature(2)430..2649
Translation

Target ORF information:

RefSeq Version XM_005268974.1
Organism Homo sapiens(Human)
Definition Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005268974.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAATGT GCAGAGGTAG AGATACAAGT AAGCAAGAGG
AAGAGGGCAG TGGTAAAAGC AAGAGGAGAC CCGACTGTGG AGACAATGAA GCAAAGGGAA
GAGTGGATCA TGACCCATGA AGAGCACCAT GCAGCCAAAA CCCTGGGGAT TGGCAAAGCC
ATTGCTGTCT TAACCTCTGG TGGAGATGCC CAAGGTATGA ATGCTGCTGT CAGGGCTGTG
GTTCGAGTTG GTATCTTCAC CGGTGCCCGT GTCTTCTTTG TCCATGAGGG TTATCAAGGC
CTGGTGGATG GTGGAGATCA CATCAAGGAA GCCACCTGGG AGAGCGTTTC GATGATGCTT
CAGCTGGGAG GCACGGTGAT TGGAAGTGCC CGGTGCAAGG ACTTTCGGGA ACGAGAAGGA
CGACTCCGAG CTGCCTACAA CCTGGTGAAG CGTGGGATCA CCAATCTCTG TGTCATTGGG
GGTGATGGCA GCCTCACTGG GGCTGACACC TTCCGTTCTG AGTGGAGTGA CTTGTTGAGT
GACCTCCAGA AAGCAGGTAA GATCACAGAT GAGGAGGCTA CGAAGTCCAG CTACCTGAAC
ATTGTGGGCC TGGTTGGGTC AATTGACAAT GACTTCTGTG GCACCGATAT GACCATTGGC
ACTGACTCTG CCCTGCATCG GATCATGGAA ATTGTAGATG CCATCACTAC CACTGCCCAG
AGCCACCAGA GGACATTTGT GTTAGAAGTA ATGGGCCGCC ACTGTGGATA CCTGGCCCTT
GTCACCTCTC TGTCCTGTGG GGCCGACTGG GTTTTTATTC CTGAATGTCC ACCAGATGAC
GACTGGGAGG AACACCTTTG TCGCCGACTC AGCGAGACAA GGACCCGTGG TTCTCGTCTC
AACATCATCA TTGTGGCTGA GGGTGCAATT GACAAGAATG GAAAACCAAT CACCTCAGAA
GACATCAAGA ATCTGGTGGT TAAGCGTCTG GGATATGACA CCCGGGTTAC TGTCTTGGGG
CATGTGCAGA GGGGTGGGAC GCCATCAGCC TTTGACAGAA TTCTGGGCAG CAGGATGGGT
GTGGAAGCAG TGATGGCACT TTTGGAGGGG ACCCCAGATA CCCCAGCCTG TGTAGTGAGC
CTCTCTGGTA ACCAGGCTGT GCGCCTGCCC CTCATGGAAT GTGTCCAGGT GACCAAAGAT
GTGACCAAGG CCATGGATGA GAAGAAATTT GACGAAGCCC TGAAGCTGAG AGGCCGGAGC
TTCATGAACA ACTGGGAGGT GTACAAGCTT CTAGCTCATG TCAGACCCCC GGTATCTAAG
AGTGGTTCGC ACACAGTGGC TGTGATGAAC GTGGGGGCTC CGGCTGCAGG CATGAATGCT
GCTGTTCGCT CCACTGTGAG GATTGGCCTT ATCCAGGGCA ACCGAGTGCT CGTTGTCCAT
GATGGTTTCG AGGGCCTGGC CAAGGGGCAG ATAGAGGAAG CTGGCTGGAG CTATGTTGGG
GGCTGGACTG GCCAAGGTGG CTCTAAACTT GGGACTAAAA GGACTCTACC CAAGAAGAGC
TTTGAACAGA TCAGTGCCAA TATAACTAAG TTTAACATTC AGGGCCTTGT CATCATTGGG
GGCTTTGAGG CTTACACAGG GGGCCTGGAA CTGATGGAGG GCAGGAAGCA GTTTGATGAG
CTCTGCATCC CATTTGTGGT CATTCCTGCT ACAGTCTCCA ACAATGTCCC TGGCTCAGAC
TTCAGCGTTG GGGCTGACAC AGCACTCAAT ACTATCTGCA CAACCTGTGA CCGCATCAAG
CAGTCAGCAG CTGGCACCAA GCGTCGGGTG TTTATCATTG AGACTATGGG TGGCTACTGT
GGCTACCTGG CTACCATGGC TGGACTGGCA GCTGGGGCCG ATGCTGCCTA CATTTTTGAG
GAGCCCTTCA CCATTCGAGA CCTGCAGGCA AATGTTGAAC ATCTGGTGCA AAAGATGAAA
ACAACTGTGA AAAGGGGCTT GGTGTTAAGG AATGAAAAGT GCAATGAGAA CTATACCACT
GACTTCATTT TCAACCTGTA CTCTGAGGAG GGGAAGGGCA TCTTCGACAG CAGGAAGAAT
GTGCTTGGTC ACATGCAGCA GGGTGGGAGC CCAACCCCAT TTGATAGGAA TTTTGCCACT
AAGATGGGCG CCAAGGCTAT GAACTGGATG TCTGGGAAAA TCAAAGAGAG TTACCGTAAT
GGGCGGATCT TTGCCAATAC TCCAGATTCG GGCTGTGTTC TGGGGATGCG TAAGAGGGCT
CTGGTCTTCC AACCAGTGGC TGAGCTGAAG GACCAGACAG ATTTTGAGCA TCGAATCCCC
AAGGAACAGT GGTGGCTGAA ACTGAGGCCC ATCCTCAAAA TCCTAGCCAA GTACGAGATT
GACTTGGACA CTTCAGACCA TGCCCACCTG GAGCACATCA CCCGGAAGCG GTCCGGGGAA
GCTGCCGTCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu46638
Clone ID Related Accession (Same CDS sequence) XM_005268976.3 , XM_005268975.1 , XM_005268974.1 , XM_017019467.1
Accession Version XM_017019467.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2652bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product ATP-dependent 6-phosphofructokinase, muscle type isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAATGT GCAGAGGTAG AGATACAAGT AAGCAAGAGG
AAGAGGGCAG TGGTAAAAGC AAGAGGAGAC CCGACTGTGG AGACAATGAA GCAAAGGGAA
GAGTGGATCA TGACCCATGA AGAGCACCAT GCAGCCAAAA CCCTGGGGAT TGGCAAAGCC
ATTGCTGTCT TAACCTCTGG TGGAGATGCC CAAGGTATGA ATGCTGCTGT CAGGGCTGTG
GTTCGAGTTG GTATCTTCAC CGGTGCCCGT GTCTTCTTTG TCCATGAGGG TTATCAAGGC
CTGGTGGATG GTGGAGATCA CATCAAGGAA GCCACCTGGG AGAGCGTTTC GATGATGCTT
CAGCTGGGAG GCACGGTGAT TGGAAGTGCC CGGTGCAAGG ACTTTCGGGA ACGAGAAGGA
CGACTCCGAG CTGCCTACAA CCTGGTGAAG CGTGGGATCA CCAATCTCTG TGTCATTGGG
GGTGATGGCA GCCTCACTGG GGCTGACACC TTCCGTTCTG AGTGGAGTGA CTTGTTGAGT
GACCTCCAGA AAGCAGGTAA GATCACAGAT GAGGAGGCTA CGAAGTCCAG CTACCTGAAC
ATTGTGGGCC TGGTTGGGTC AATTGACAAT GACTTCTGTG GCACCGATAT GACCATTGGC
ACTGACTCTG CCCTGCATCG GATCATGGAA ATTGTAGATG CCATCACTAC CACTGCCCAG
AGCCACCAGA GGACATTTGT GTTAGAAGTA ATGGGCCGCC ACTGTGGATA CCTGGCCCTT
GTCACCTCTC TGTCCTGTGG GGCCGACTGG GTTTTTATTC CTGAATGTCC ACCAGATGAC
GACTGGGAGG AACACCTTTG TCGCCGACTC AGCGAGACAA GGACCCGTGG TTCTCGTCTC
AACATCATCA TTGTGGCTGA GGGTGCAATT GACAAGAATG GAAAACCAAT CACCTCAGAA
GACATCAAGA ATCTGGTGGT TAAGCGTCTG GGATATGACA CCCGGGTTAC TGTCTTGGGG
CATGTGCAGA GGGGTGGGAC GCCATCAGCC TTTGACAGAA TTCTGGGCAG CAGGATGGGT
GTGGAAGCAG TGATGGCACT TTTGGAGGGG ACCCCAGATA CCCCAGCCTG TGTAGTGAGC
CTCTCTGGTA ACCAGGCTGT GCGCCTGCCC CTCATGGAAT GTGTCCAGGT GACCAAAGAT
GTGACCAAGG CCATGGATGA GAAGAAATTT GACGAAGCCC TGAAGCTGAG AGGCCGGAGC
TTCATGAACA ACTGGGAGGT GTACAAGCTT CTAGCTCATG TCAGACCCCC GGTATCTAAG
AGTGGTTCGC ACACAGTGGC TGTGATGAAC GTGGGGGCTC CGGCTGCAGG CATGAATGCT
GCTGTTCGCT CCACTGTGAG GATTGGCCTT ATCCAGGGCA ACCGAGTGCT CGTTGTCCAT
GATGGTTTCG AGGGCCTGGC CAAGGGGCAG ATAGAGGAAG CTGGCTGGAG CTATGTTGGG
GGCTGGACTG GCCAAGGTGG CTCTAAACTT GGGACTAAAA GGACTCTACC CAAGAAGAGC
TTTGAACAGA TCAGTGCCAA TATAACTAAG TTTAACATTC AGGGCCTTGT CATCATTGGG
GGCTTTGAGG CTTACACAGG GGGCCTGGAA CTGATGGAGG GCAGGAAGCA GTTTGATGAG
CTCTGCATCC CATTTGTGGT CATTCCTGCT ACAGTCTCCA ACAATGTCCC TGGCTCAGAC
TTCAGCGTTG GGGCTGACAC AGCACTCAAT ACTATCTGCA CAACCTGTGA CCGCATCAAG
CAGTCAGCAG CTGGCACCAA GCGTCGGGTG TTTATCATTG AGACTATGGG TGGCTACTGT
GGCTACCTGG CTACCATGGC TGGACTGGCA GCTGGGGCCG ATGCTGCCTA CATTTTTGAG
GAGCCCTTCA CCATTCGAGA CCTGCAGGCA AATGTTGAAC ATCTGGTGCA AAAGATGAAA
ACAACTGTGA AAAGGGGCTT GGTGTTAAGG AATGAAAAGT GCAATGAGAA CTATACCACT
GACTTCATTT TCAACCTGTA CTCTGAGGAG GGGAAGGGCA TCTTCGACAG CAGGAAGAAT
GTGCTTGGTC ACATGCAGCA GGGTGGGAGC CCAACCCCAT TTGATAGGAA TTTTGCCACT
AAGATGGGCG CCAAGGCTAT GAACTGGATG TCTGGGAAAA TCAAAGAGAG TTACCGTAAT
GGGCGGATCT TTGCCAATAC TCCAGATTCG GGCTGTGTTC TGGGGATGCG TAAGAGGGCT
CTGGTCTTCC AACCAGTGGC TGAGCTGAAG GACCAGACAG ATTTTGAGCA TCGAATCCCC
AAGGAACAGT GGTGGCTGAA ACTGAGGCCC ATCCTCAAAA TCCTAGCCAA GTACGAGATT
GACTTGGACA CTTCAGACCA TGCCCACCTG GAGCACATCA CCCGGAAGCG GTCCGGGGAA
GCTGCCGTCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016874956.1
CDS145..2796
Misc Feature(1)490..2775
Misc Feature(2)499..2718
Translation

Target ORF information:

RefSeq Version XM_017019467.1
Organism Homo sapiens(Human)
Definition Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017019467.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAATGT GCAGAGGTAG AGATACAAGT AAGCAAGAGG
AAGAGGGCAG TGGTAAAAGC AAGAGGAGAC CCGACTGTGG AGACAATGAA GCAAAGGGAA
GAGTGGATCA TGACCCATGA AGAGCACCAT GCAGCCAAAA CCCTGGGGAT TGGCAAAGCC
ATTGCTGTCT TAACCTCTGG TGGAGATGCC CAAGGTATGA ATGCTGCTGT CAGGGCTGTG
GTTCGAGTTG GTATCTTCAC CGGTGCCCGT GTCTTCTTTG TCCATGAGGG TTATCAAGGC
CTGGTGGATG GTGGAGATCA CATCAAGGAA GCCACCTGGG AGAGCGTTTC GATGATGCTT
CAGCTGGGAG GCACGGTGAT TGGAAGTGCC CGGTGCAAGG ACTTTCGGGA ACGAGAAGGA
CGACTCCGAG CTGCCTACAA CCTGGTGAAG CGTGGGATCA CCAATCTCTG TGTCATTGGG
GGTGATGGCA GCCTCACTGG GGCTGACACC TTCCGTTCTG AGTGGAGTGA CTTGTTGAGT
GACCTCCAGA AAGCAGGTAA GATCACAGAT GAGGAGGCTA CGAAGTCCAG CTACCTGAAC
ATTGTGGGCC TGGTTGGGTC AATTGACAAT GACTTCTGTG GCACCGATAT GACCATTGGC
ACTGACTCTG CCCTGCATCG GATCATGGAA ATTGTAGATG CCATCACTAC CACTGCCCAG
AGCCACCAGA GGACATTTGT GTTAGAAGTA ATGGGCCGCC ACTGTGGATA CCTGGCCCTT
GTCACCTCTC TGTCCTGTGG GGCCGACTGG GTTTTTATTC CTGAATGTCC ACCAGATGAC
GACTGGGAGG AACACCTTTG TCGCCGACTC AGCGAGACAA GGACCCGTGG TTCTCGTCTC
AACATCATCA TTGTGGCTGA GGGTGCAATT GACAAGAATG GAAAACCAAT CACCTCAGAA
GACATCAAGA ATCTGGTGGT TAAGCGTCTG GGATATGACA CCCGGGTTAC TGTCTTGGGG
CATGTGCAGA GGGGTGGGAC GCCATCAGCC TTTGACAGAA TTCTGGGCAG CAGGATGGGT
GTGGAAGCAG TGATGGCACT TTTGGAGGGG ACCCCAGATA CCCCAGCCTG TGTAGTGAGC
CTCTCTGGTA ACCAGGCTGT GCGCCTGCCC CTCATGGAAT GTGTCCAGGT GACCAAAGAT
GTGACCAAGG CCATGGATGA GAAGAAATTT GACGAAGCCC TGAAGCTGAG AGGCCGGAGC
TTCATGAACA ACTGGGAGGT GTACAAGCTT CTAGCTCATG TCAGACCCCC GGTATCTAAG
AGTGGTTCGC ACACAGTGGC TGTGATGAAC GTGGGGGCTC CGGCTGCAGG CATGAATGCT
GCTGTTCGCT CCACTGTGAG GATTGGCCTT ATCCAGGGCA ACCGAGTGCT CGTTGTCCAT
GATGGTTTCG AGGGCCTGGC CAAGGGGCAG ATAGAGGAAG CTGGCTGGAG CTATGTTGGG
GGCTGGACTG GCCAAGGTGG CTCTAAACTT GGGACTAAAA GGACTCTACC CAAGAAGAGC
TTTGAACAGA TCAGTGCCAA TATAACTAAG TTTAACATTC AGGGCCTTGT CATCATTGGG
GGCTTTGAGG CTTACACAGG GGGCCTGGAA CTGATGGAGG GCAGGAAGCA GTTTGATGAG
CTCTGCATCC CATTTGTGGT CATTCCTGCT ACAGTCTCCA ACAATGTCCC TGGCTCAGAC
TTCAGCGTTG GGGCTGACAC AGCACTCAAT ACTATCTGCA CAACCTGTGA CCGCATCAAG
CAGTCAGCAG CTGGCACCAA GCGTCGGGTG TTTATCATTG AGACTATGGG TGGCTACTGT
GGCTACCTGG CTACCATGGC TGGACTGGCA GCTGGGGCCG ATGCTGCCTA CATTTTTGAG
GAGCCCTTCA CCATTCGAGA CCTGCAGGCA AATGTTGAAC ATCTGGTGCA AAAGATGAAA
ACAACTGTGA AAAGGGGCTT GGTGTTAAGG AATGAAAAGT GCAATGAGAA CTATACCACT
GACTTCATTT TCAACCTGTA CTCTGAGGAG GGGAAGGGCA TCTTCGACAG CAGGAAGAAT
GTGCTTGGTC ACATGCAGCA GGGTGGGAGC CCAACCCCAT TTGATAGGAA TTTTGCCACT
AAGATGGGCG CCAAGGCTAT GAACTGGATG TCTGGGAAAA TCAAAGAGAG TTACCGTAAT
GGGCGGATCT TTGCCAATAC TCCAGATTCG GGCTGTGTTC TGGGGATGCG TAAGAGGGCT
CTGGTCTTCC AACCAGTGGC TGAGCTGAAG GACCAGACAG ATTTTGAGCA TCGAATCCCC
AAGGAACAGT GGTGGCTGAA ACTGAGGCCC ATCCTCAAAA TCCTAGCCAA GTACGAGATT
GACTTGGACA CTTCAGACCA TGCCCACCTG GAGCACATCA CCCGGAAGCG GTCCGGGGAA
GCTGCCGTCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59637
Clone ID Related Accession (Same CDS sequence) XM_011538487.1
Accession Version XM_011538487.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2559bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product ATP-dependent 6-phosphofructokinase, muscle type isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAATGT GCAGAGGTAG AGATACAAGT AAGCAAGAGG
AAGAGGGCAG TGGTAAAAGC AAGAGGAGAC CCGACTGTGG AGACAATGAA GCAAAGGGAA
GAGTGGATCA TGACCCATGA AGAGCACCAT GCAGCCAAAA CCCTGGGGAT TGGCAAAGCC
ATTGCTGTCT TAACCTCTGG TGGAGATGCC CAAGGTATGA ATGCTGCTGT CAGGGCTGTG
GTTCGAGTTG GTATCTTCAC CGGTGCCCGT GTCTTCTTTG TCCATGAGGG TTATCAAGGC
CTGGTGGATG GTGGAGATCA CATCAAGGAA GCCACCTGGG AGAGCGTTTC GATGATGCTT
CAGCTGGGAG GCACGGTGAT TGGAAGTGCC CGGTGCAAGG ACTTTCGGGA ACGAGAAGGA
CGACTCCGAG CTGCCTACAA CCTGGTGAAG CGTGGGATCA CCAATCTCTG TGTCATTGGG
GGTGATGGCA GCCTCACTGG GGCTGACACC TTCCGTTCTG AGTGGAGTGA CTTGTTGAGT
GACCTCCAGA AAGCAGGTAA GATCACAGAT GAGGAGGCTA CGAAGTCCAG CTACCTGAAC
ATTGTGGGCC TGGTTGGGTC AATTGACAAT GACTTCTGTG GCACCGATAT GACCATTGGC
ACTGACTCTG CCCTGCATCG GATCATGGAA ATTGTAGATG CCATCACTAC CACTGCCCAG
AGCCACCAGA GGACATTTGT GTTAGAAGTA ATGGGCCGCC ACTGTGGATA CCTGGCCCTT
GTCACCTCTC TGTCCTGTGG GGCCGACTGG GTTTTTATTC CTGAATGTCC ACCAGATGAC
GACTGGGAGG AACACCTTTG TCGCCGACTC AGCGAGACAA GGACCCGTGG TTCTCGTCTC
AACATCATCA TTGTGGCTGA GGGTGCAATT GACAAGAATG GAAAACCAAT CACCTCAGAA
GACATCAAGA ATGGCAGCAG GATGGGTGTG GAAGCAGTGA TGGCACTTTT GGAGGGGACC
CCAGATACCC CAGCCTGTGT AGTGAGCCTC TCTGGTAACC AGGCTGTGCG CCTGCCCCTC
ATGGAATGTG TCCAGGTGAC CAAAGATGTG ACCAAGGCCA TGGATGAGAA GAAATTTGAC
GAAGCCCTGA AGCTGAGAGG CCGGAGCTTC ATGAACAACT GGGAGGTGTA CAAGCTTCTA
GCTCATGTCA GACCCCCGGT ATCTAAGAGT GGTTCGCACA CAGTGGCTGT GATGAACGTG
GGGGCTCCGG CTGCAGGCAT GAATGCTGCT GTTCGCTCCA CTGTGAGGAT TGGCCTTATC
CAGGGCAACC GAGTGCTCGT TGTCCATGAT GGTTTCGAGG GCCTGGCCAA GGGGCAGATA
GAGGAAGCTG GCTGGAGCTA TGTTGGGGGC TGGACTGGCC AAGGTGGCTC TAAACTTGGG
ACTAAAAGGA CTCTACCCAA GAAGAGCTTT GAACAGATCA GTGCCAATAT AACTAAGTTT
AACATTCAGG GCCTTGTCAT CATTGGGGGC TTTGAGGCTT ACACAGGGGG CCTGGAACTG
ATGGAGGGCA GGAAGCAGTT TGATGAGCTC TGCATCCCAT TTGTGGTCAT TCCTGCTACA
GTCTCCAACA ATGTCCCTGG CTCAGACTTC AGCGTTGGGG CTGACACAGC ACTCAATACT
ATCTGCACAA CCTGTGACCG CATCAAGCAG TCAGCAGCTG GCACCAAGCG TCGGGTGTTT
ATCATTGAGA CTATGGGTGG CTACTGTGGC TACCTGGCTA CCATGGCTGG ACTGGCAGCT
GGGGCCGATG CTGCCTACAT TTTTGAGGAG CCCTTCACCA TTCGAGACCT GCAGGCAAAT
GTTGAACATC TGGTGCAAAA GATGAAAACA ACTGTGAAAA GGGGCTTGGT GTTAAGGAAT
GAAAAGTGCA ATGAGAACTA TACCACTGAC TTCATTTTCA ACCTGTACTC TGAGGAGGGG
AAGGGCATCT TCGACAGCAG GAAGAATGTG CTTGGTCACA TGCAGCAGGG TGGGAGCCCA
ACCCCATTTG ATAGGAATTT TGCCACTAAG ATGGGCGCCA AGGCTATGAA CTGGATGTCT
GGGAAAATCA AAGAGAGTTA CCGTAATGGG CGGATCTTTG CCAATACTCC AGATTCGGGC
TGTGTTCTGG GGATGCGTAA GAGGGCTCTG GTCTTCCAAC CAGTGGCTGA GCTGAAGGAC
CAGACAGATT TTGAGCATCG AATCCCCAAG GAACAGTGGT GGCTGAAACT GAGGCCCATC
CTCAAAATCC TAGCCAAGTA CGAGATTGAC TTGGACACTT CAGACCATGC CCACCTGGAG
CACATCACCC GGAAGCGGTC CGGGGAAGCT GCCGTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011536789.1
CDS10..2568
Misc Feature(1)355..2547
Misc Feature(2)364..2490
Translation

Target ORF information:

RefSeq Version XM_011538487.1
Organism Homo sapiens(Human)
Definition Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011538487.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAATGT GCAGAGGTAG AGATACAAGT AAGCAAGAGG
AAGAGGGCAG TGGTAAAAGC AAGAGGAGAC CCGACTGTGG AGACAATGAA GCAAAGGGAA
GAGTGGATCA TGACCCATGA AGAGCACCAT GCAGCCAAAA CCCTGGGGAT TGGCAAAGCC
ATTGCTGTCT TAACCTCTGG TGGAGATGCC CAAGGTATGA ATGCTGCTGT CAGGGCTGTG
GTTCGAGTTG GTATCTTCAC CGGTGCCCGT GTCTTCTTTG TCCATGAGGG TTATCAAGGC
CTGGTGGATG GTGGAGATCA CATCAAGGAA GCCACCTGGG AGAGCGTTTC GATGATGCTT
CAGCTGGGAG GCACGGTGAT TGGAAGTGCC CGGTGCAAGG ACTTTCGGGA ACGAGAAGGA
CGACTCCGAG CTGCCTACAA CCTGGTGAAG CGTGGGATCA CCAATCTCTG TGTCATTGGG
GGTGATGGCA GCCTCACTGG GGCTGACACC TTCCGTTCTG AGTGGAGTGA CTTGTTGAGT
GACCTCCAGA AAGCAGGTAA GATCACAGAT GAGGAGGCTA CGAAGTCCAG CTACCTGAAC
ATTGTGGGCC TGGTTGGGTC AATTGACAAT GACTTCTGTG GCACCGATAT GACCATTGGC
ACTGACTCTG CCCTGCATCG GATCATGGAA ATTGTAGATG CCATCACTAC CACTGCCCAG
AGCCACCAGA GGACATTTGT GTTAGAAGTA ATGGGCCGCC ACTGTGGATA CCTGGCCCTT
GTCACCTCTC TGTCCTGTGG GGCCGACTGG GTTTTTATTC CTGAATGTCC ACCAGATGAC
GACTGGGAGG AACACCTTTG TCGCCGACTC AGCGAGACAA GGACCCGTGG TTCTCGTCTC
AACATCATCA TTGTGGCTGA GGGTGCAATT GACAAGAATG GAAAACCAAT CACCTCAGAA
GACATCAAGA ATGGCAGCAG GATGGGTGTG GAAGCAGTGA TGGCACTTTT GGAGGGGACC
CCAGATACCC CAGCCTGTGT AGTGAGCCTC TCTGGTAACC AGGCTGTGCG CCTGCCCCTC
ATGGAATGTG TCCAGGTGAC CAAAGATGTG ACCAAGGCCA TGGATGAGAA GAAATTTGAC
GAAGCCCTGA AGCTGAGAGG CCGGAGCTTC ATGAACAACT GGGAGGTGTA CAAGCTTCTA
GCTCATGTCA GACCCCCGGT ATCTAAGAGT GGTTCGCACA CAGTGGCTGT GATGAACGTG
GGGGCTCCGG CTGCAGGCAT GAATGCTGCT GTTCGCTCCA CTGTGAGGAT TGGCCTTATC
CAGGGCAACC GAGTGCTCGT TGTCCATGAT GGTTTCGAGG GCCTGGCCAA GGGGCAGATA
GAGGAAGCTG GCTGGAGCTA TGTTGGGGGC TGGACTGGCC AAGGTGGCTC TAAACTTGGG
ACTAAAAGGA CTCTACCCAA GAAGAGCTTT GAACAGATCA GTGCCAATAT AACTAAGTTT
AACATTCAGG GCCTTGTCAT CATTGGGGGC TTTGAGGCTT ACACAGGGGG CCTGGAACTG
ATGGAGGGCA GGAAGCAGTT TGATGAGCTC TGCATCCCAT TTGTGGTCAT TCCTGCTACA
GTCTCCAACA ATGTCCCTGG CTCAGACTTC AGCGTTGGGG CTGACACAGC ACTCAATACT
ATCTGCACAA CCTGTGACCG CATCAAGCAG TCAGCAGCTG GCACCAAGCG TCGGGTGTTT
ATCATTGAGA CTATGGGTGG CTACTGTGGC TACCTGGCTA CCATGGCTGG ACTGGCAGCT
GGGGCCGATG CTGCCTACAT TTTTGAGGAG CCCTTCACCA TTCGAGACCT GCAGGCAAAT
GTTGAACATC TGGTGCAAAA GATGAAAACA ACTGTGAAAA GGGGCTTGGT GTTAAGGAAT
GAAAAGTGCA ATGAGAACTA TACCACTGAC TTCATTTTCA ACCTGTACTC TGAGGAGGGG
AAGGGCATCT TCGACAGCAG GAAGAATGTG CTTGGTCACA TGCAGCAGGG TGGGAGCCCA
ACCCCATTTG ATAGGAATTT TGCCACTAAG ATGGGCGCCA AGGCTATGAA CTGGATGTCT
GGGAAAATCA AAGAGAGTTA CCGTAATGGG CGGATCTTTG CCAATACTCC AGATTCGGGC
TGTGTTCTGG GGATGCGTAA GAGGGCTCTG GTCTTCCAAC CAGTGGCTGA GCTGAAGGAC
CAGACAGATT TTGAGCATCG AATCCCCAAG GAACAGTGGT GGCTGAAACT GAGGCCCATC
CTCAAAATCC TAGCCAAGTA CGAGATTGAC TTGGACACTT CAGACCATGC CCACCTGGAG
CACATCACCC GGAAGCGGTC CGGGGAAGCT GCCGTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu83887
Clone ID Related Accession (Same CDS sequence) XM_017019469.1
Accession Version XM_017019469.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2463bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product ATP-dependent 6-phosphofructokinase, muscle type isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATGGCA GCAGGATGGG TGTGGAAGCA
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016874958.1
CDS158..2620
Translation

Target ORF information:

RefSeq Version XM_017019469.1
Organism Homo sapiens(Human)
Definition Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X10, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017019469.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATGGCA GCAGGATGGG TGTGGAAGCA
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Cell164(4)805-17(2016 Feb)
Yang X,Coulombe-Huntington J,Kang S,Sheynkman GM,Hao T,Richardson A,Sun S,Yang F,Shen YA,Murray RR,Spirohn K,Begg BE,Duran-Frigola M,MacWilliams A,Pevzner SJ,Zhong Q,Trigg SA,Tam S,Ghamsari L,Sahni N,Yi S,Rodriguez MD,Balcha D,Tan G,Costanzo M,Andrews B,Boone C,Zhou XJ,Salehi-Ashtiani K,Charloteaux B,Chen AA,Calderwood MA,Aloy P,Roth FP,Hill DE,Iakoucheva LM,Xia Y,Vidal M


book

The expression pattern of PFKFB3 enzyme distinguishes between induced-pluripotent stem cells and cancer stem cells.
Oncotarget6(30)29753-70(2015 Oct)
Cieślar-Pobuda A,Jain MV,Kratz G,Rzeszowska-Wolny J,Ghavami S,Wiechec E


book

Structures of human phosphofructokinase-1 and atomic basis of cancer-associated mutations.
Nature523(7558)111-4(2015 Jul)
Webb BA,Forouhar F,Szu FE,Seetharaman J,Tong L,Barber DL


book

Novel testis- and embryo-specific isoforms of the phosphofructokinase-1 muscle type gene.
Biochemical and biophysical research communications316(2)580-7(2004 Apr)
Yamada S,Nakajima H,Kuehn MR


book

Physical and genetic mapping of the muscle phosphofructokinase gene (PFKM): reassignment to human chromosome 12q.
Genomics34(1)122-7(1996 May)
Howard TD,Akots G,Bowden DW


 
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