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PGK1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol PGK1
Entrez Gene ID 5230
Full Name phosphoglycerate kinase 1
Synonyms HEL-S-68p, MIG10, PGKA
General protein information
Preferred Names
phosphoglycerate kinase 1
Names
phosphoglycerate kinase 1
PRP 2
primer recognition protein 2
cell migration-inducing gene 10 protein
NP_000282.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

X

Xq13.3

Summary The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome. [provided by RefSeq, Jan 2014]. lac of sum
Disorder MIM:

311800

Disorder Html: Phosphoglycerate kinase 1 deficiency, 300653 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_000291 NP_000282 phosphoglycerate kinase 1



Homo sapiens (human) PGK1 NP_000282.1
Pan troglodytes (chimpanzee) PGK1 NP_001129210.1
Macaca mulatta (Rhesus monkey) PGK1 XP_001100787.1
Canis lupus familiaris (dog) PGK1 XP_849992.1
Bos taurus (cattle) PGK1 NP_001029471.1
Mus musculus (house mouse) Pgk1 NP_032854.2
Rattus norvegicus (Norway rat) Pgk1 NP_445743.2
Gallus gallus (chicken) PGK1 NP_990316.1
Danio rerio (zebrafish) pgk1 NP_998552.1
Drosophila melanogaster (fruit fly) Pgk NP_001259958.1
Caenorhabditis elegans pgk-1 NP_491245.1
Arabidopsis thaliana (thale cress) PGK1 NP_187884.1
Arabidopsis thaliana (thale cress) AT1G56190 NP_176015.1
Xenopus (Silurana) tropicalis (western clawed frog) pgk1 NP_001016545.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PGK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PGK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu19088
NM_000291 Homo sapiens phosphoglycerate kinase 1 (PGK1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu19088
Clone ID Related Accession (Same CDS sequence) NM_000291
Accession Version NM_000291.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1254bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product phosphoglycerate kinase 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC336483.1, BC103752.1 and BU677171.1. This sequence is a reference standard in the RefSeqGene project. On Apr 18, 2008 this sequence version replaced gi:22095338. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 2324090, 11130727 ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AB062432.1, BC113568.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTCGCTTT CTAACAAGCT GACGCTGGAC AAGCTGGACG TTAAAGGGAA GCGGGTCGTT 
ATGAGAGTCG ACTTCAATGT TCCTATGAAG AACAACCAGA TAACAAACAA CCAGAGGATT
AAGGCTGCTG TCCCAAGCAT CAAATTCTGC TTGGACAATG GAGCCAAGTC GGTAGTCCTT
ATGAGCCACC TAGGCCGGCC TGATGGTGTG CCCATGCCTG ACAAGTACTC CTTAGAGCCA
GTTGCTGTAG AACTCAAATC TCTGCTGGGC AAGGATGTTC TGTTCTTGAA GGACTGTGTA
GGCCCAGAAG TGGAGAAAGC CTGTGCCAAC CCAGCTGCTG GGTCTGTCAT CCTGCTGGAG
AACCTCCGCT TTCATGTGGA GGAAGAAGGG AAGGGAAAAG ATGCTTCTGG GAACAAGGTT
AAAGCCGAGC CAGCCAAAAT AGAAGCTTTC CGAGCTTCAC TTTCCAAGCT AGGGGATGTC
TATGTCAATG ATGCTTTTGG CACTGCTCAC AGAGCCCACA GCTCCATGGT AGGAGTCAAT
CTGCCACAGA AGGCTGGTGG GTTTTTGATG AAGAAGGAGC TGAACTACTT TGCAAAGGCC
TTGGAGAGCC CAGAGCGACC CTTCCTGGCC ATCCTGGGCG GAGCTAAAGT TGCAGACAAG
ATCCAGCTCA TCAATAATAT GCTGGACAAA GTCAATGAGA TGATTATTGG TGGTGGAATG
GCTTTTACCT TCCTTAAGGT GCTCAACAAC ATGGAGATTG GCACTTCTCT GTTTGATGAA
GAGGGAGCCA AGATTGTCAA AGACCTAATG TCCAAAGCTG AGAAGAATGG TGTGAAGATT
ACCTTGCCTG TTGACTTTGT CACTGCTGAC AAGTTTGATG AGAATGCCAA GACTGGCCAA
GCCACTGTGG CTTCTGGCAT ACCTGCTGGC TGGATGGGCT TGGACTGTGG TCCTGAAAGC
AGCAAGAAGT ATGCTGAGGC TGTCACTCGG GCTAAGCAGA TTGTGTGGAA TGGTCCTGTG
GGGGTATTTG AATGGGAAGC TTTTGCCCGG GGAACCAAAG CTCTCATGGA TGAGGTGGTG
AAAGCCACTT CTAGGGGCTG CATCACCATC ATAGGTGGTG GAGACACTGC CACTTGCTGT
GCCAAATGGA ACACGGAGGA TAAAGTCAGC CATGTGAGCA CTGGGGGTGG TGCCAGTTTG
GAGCTCCTGG AAGGTAAAGT CCTTCCTGGG GTGGATGCTC TCAGCAATAT TTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000282.1
CDS173..1426
Misc Feature(1)50..52(+)
Misc Feature(2)176..178(+)
Misc Feature(3)197..1420(+)
Misc Feature(4)203..205(+)
Misc Feature(5)242..541(+)
Misc Feature(6)242..250(+)
Misc Feature(7)314..316(+)
Misc Feature(8)359..370(+)
Misc Feature(9)395..397(+)
Misc Feature(10)428..430(+)
Misc Feature(11)461..463(+)
Misc Feature(12)563..565(+)
Misc Feature(13)563..565(+)
Misc Feature(14)608..610(+)
Misc Feature(15)758..760(+)
Misc Feature(16)758..760(+)
Misc Feature(17)767..769(+)
Misc Feature(18)773..1354(+)
Misc Feature(19)779..781(+)
Misc Feature(20)779..781(+)
Misc Feature(21)887..1300(+)
Misc Feature(22)971..973(+)
Misc Feature(23)1043..1045(+)
Misc Feature(24)1295..1297(+)
Exon (1)1..237
Gene:PGK1
Gene Synonym:
Exon (2)238..288
Gene:PGK1
Gene Synonym:
Exon (3)289..444
Gene:PGK1
Gene Synonym:
Exon (4)445..589
Gene:PGK1
Gene Synonym:
Exon (5)590..693
Gene:PGK1
Gene Synonym:
Exon (6)694..813
Gene:PGK1
Gene Synonym:
Exon (7)814..928
Gene:PGK1
Gene Synonym:
Exon (8)929..1108
Gene:PGK1
Gene Synonym:
Exon (9)1109..1286
Gene:PGK1
Gene Synonym:
Exon (10)1287..1385
Gene:PGK1
Gene Synonym:
Exon (11)1386..2423
Gene:PGK1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000291
Organism Homo sapiens (human)
Definition Homo sapiens phosphoglycerate kinase 1 (PGK1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000291

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTCGCTTT CTAACAAGCT GACGCTGGAC AAGCTGGACG TTAAAGGGAA GCGGGTCGTT 
ATGAGAGTCG ACTTCAATGT TCCTATGAAG AACAACCAGA TAACAAACAA CCAGAGGATT
AAGGCTGCTG TCCCAAGCAT CAAATTCTGC TTGGACAATG GAGCCAAGTC GGTAGTCCTT
ATGAGCCACC TAGGCCGGCC TGATGGTGTG CCCATGCCTG ACAAGTACTC CTTAGAGCCA
GTTGCTGTAG AACTCAAATC TCTGCTGGGC AAGGATGTTC TGTTCTTGAA GGACTGTGTA
GGCCCAGAAG TGGAGAAAGC CTGTGCCAAC CCAGCTGCTG GGTCTGTCAT CCTGCTGGAG
AACCTCCGCT TTCATGTGGA GGAAGAAGGG AAGGGAAAAG ATGCTTCTGG GAACAAGGTT
AAAGCCGAGC CAGCCAAAAT AGAAGCTTTC CGAGCTTCAC TTTCCAAGCT AGGGGATGTC
TATGTCAATG ATGCTTTTGG CACTGCTCAC AGAGCCCACA GCTCCATGGT AGGAGTCAAT
CTGCCACAGA AGGCTGGTGG GTTTTTGATG AAGAAGGAGC TGAACTACTT TGCAAAGGCC
TTGGAGAGCC CAGAGCGACC CTTCCTGGCC ATCCTGGGCG GAGCTAAAGT TGCAGACAAG
ATCCAGCTCA TCAATAATAT GCTGGACAAA GTCAATGAGA TGATTATTGG TGGTGGAATG
GCTTTTACCT TCCTTAAGGT GCTCAACAAC ATGGAGATTG GCACTTCTCT GTTTGATGAA
GAGGGAGCCA AGATTGTCAA AGACCTAATG TCCAAAGCTG AGAAGAATGG TGTGAAGATT
ACCTTGCCTG TTGACTTTGT CACTGCTGAC AAGTTTGATG AGAATGCCAA GACTGGCCAA
GCCACTGTGG CTTCTGGCAT ACCTGCTGGC TGGATGGGCT TGGACTGTGG TCCTGAAAGC
AGCAAGAAGT ATGCTGAGGC TGTCACTCGG GCTAAGCAGA TTGTGTGGAA TGGTCCTGTG
GGGGTATTTG AATGGGAAGC TTTTGCCCGG GGAACCAAAG CTCTCATGGA TGAGGTGGTG
AAAGCCACTT CTAGGGGCTG CATCACCATC ATAGGTGGTG GAGACACTGC CACTTGCTGT
GCCAAATGGA ACACGGAGGA TAAAGTCAGC CATGTGAGCA CTGGGGGTGG TGCCAGTTTG
GAGCTCCTGG AAGGTAAAGT CCTTCCTGGG GTGGATGCTC TCAGCAATAT TTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Phosphoglycerate kinase 1 promotes radioresistance in U251 human glioma cells
Oncol. Rep. 31 (2), 894-900 (2014)
Ding H, Cheng YJ, Yan H, Zhang R, Zhao JB, Qian CF, Zhang WB, Xiao H and Liu HY.


book

PGK1 as predictor of CXCR4 expression, bone marrow metastases and survival in neuroblastoma
PLoS ONE 8 (12), E83701 (2013)
Ameis HM, Drenckhan A, von Loga K, Escherich G, Wenke K, Izbicki JR, Reinshagen K and Gros SJ.


book

Phosphoglycerate kinase 1 as a promoter of metastasis in colon cancer
Int. J. Oncol. 43 (2), 586-590 (2013)
Ahmad SS, Glatzle J, Bajaeifer K, Buhler S, Lehmann T, Konigsrainer I, Vollmer JP, Sipos B, Ahmad SS, Northoff H, Konigsrainer A and Zieker D.


book

Conformational dynamics in phosphoglycerate kinase, an open and shut case?
FEBS Lett. 587 (13), 1878-1883 (2013)
Bowler MW.


book

Phosphoglycerate kinase acts in tumour angiogenesis as a disulphide reductase
Nature 408 (6814), 869-873 (2000)
Lay AJ, Jiang XM, Kisker O, Flynn E, Underwood A, Condron R and Hogg PJ.


book

Molecular abnormalities of a phosphoglycerate kinase variant generated by spontaneous mutation
Blood 79 (10), 2759-2762 (1992)
Maeda M, Bawle EV, Kulkarni R, Beutler E and Yoshida A.


book

Treatment of Haemophilus aphrophilus endocarditis with ciprofloxacin
J. Infect. 24 (3), 317-320 (1992)
Dawson SJ and White LA.


book

A single amino acid substitution (157 Gly----Val) in a phosphoglycerate kinase variant (PGK Shizuoka) associated with chronic hemolysis and myoglobinuria
Blood 79 (6), 1582-1585 (1992)
Fujii H, Kanno H, Hirono A, Shiomura T and Miwa S.


book

The role of primer recognition proteins in DNA replication: association with nuclear matrix in HeLa cells
J. Cell. Sci. 101 (PT 1), 25-34 (1992)
Vishwanatha JK, Jindal HK and Davis RG.


book

Functional identity of a primer recognition protein as phosphoglycerate kinase
J. Biol. Chem. 265 (12), 6540-6543 (1990)
Jindal HK and Vishwanatha JK.


 
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