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PHKA2 cDNA ORF clone, Homo sapiens (human)

Gene Symbol PHKA2
Entrez Gene ID 5256
Full Name phosphorylase kinase, alpha 2 (liver)
Synonyms GSD9A, PHK, PYK, PYKL, XLG, XLG2
General protein information
Preferred Names
phosphorylase b kinase regulatory subunit alpha, liver isoform
Names
phosphorylase b kinase regulatory subunit alpha, liver isoform
phosphorylase kinase alpha-subunit
phosphorylase kinase alpha L subunit
phosphorylase b kinase regulatory subunit alpha liver isoform
NP_000283.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

X

Xp22.2-p22.1

Summary Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, and the hepatic isoform is encoded by this gene. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, which are encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9A, also known as X-linked liver glycogenosis. Alternatively spliced transcript variants have been reported, but the full-length nature of these variants has not been determined.[provided by RefSeq, Feb 2010]. lac of sum
Disorder MIM:

300798

Disorder Html: Glycogen storage disease, type IXa1, 306000 (3); Glycogen storage

mRNA and Protein(s)

mRNA Protein Name
XM_006724496 XP_006724559 phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X1
XM_005274548 XP_005274605 phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X2
XM_011545537 XP_011543839 phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X3
XM_005274550 XP_005274607 phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X4
XM_006724498 XP_006724561 phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X5
XM_011545538 XP_011543840 phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X6
NM_000292 NP_000283 phosphorylase b kinase regulatory subunit alpha, liver isoform



Homo sapiens (human) PHKA2 NP_000283.1
Pan troglodytes (chimpanzee) PHKA2 XP_003317431.1
Macaca mulatta (Rhesus monkey) PHKA2 XP_001084454.1
Canis lupus familiaris (dog) PHKA2 XP_005641224.1
Bos taurus (cattle) PHKA2 NP_001178474.1
Mus musculus (house mouse) Phka2 NP_001171350.1
Rattus norvegicus (Norway rat) Phka2 NP_001177923.1
Gallus gallus (chicken) PHKA2 XP_004934744.1
Danio rerio (zebrafish) LOC556199 XP_684044.4
Drosophila melanogaster (fruit fly) CG7766 NP_001138180.1
Caenorhabditis elegans C14B9.8 NP_498777.2
Xenopus (Silurana) tropicalis (western clawed frog) phka2 XP_004911720.1


Related articles in PubMed

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PHKA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PHKA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu53929 XM_006724496 PREDICTED: Homo sapiens phosphorylase kinase, alpha 2 (liver) (PHKA2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu53931 XM_005274548 PREDICTED: Homo sapiens phosphorylase kinase, alpha 2 (liver) (PHKA2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu59671 XM_011545537 PREDICTED: Homo sapiens phosphorylase kinase, alpha 2 (liver) (PHKA2), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu53932 XM_005274550 PREDICTED: Homo sapiens phosphorylase kinase, alpha 2 (liver) (PHKA2), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu53933 XM_006724498 PREDICTED: Homo sapiens phosphorylase kinase, alpha 2 (liver) (PHKA2), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu59672 XM_011545538 PREDICTED: Homo sapiens phosphorylase kinase, alpha 2 (liver) (PHKA2), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu18889
NM_000292 Homo sapiens phosphorylase kinase, alpha 2 (liver) (PHKA2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu53929
Clone ID Related Accession (Same CDS sequence) XM_006724496
Accession Version XM_006724496.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3732bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167197.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578837913. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGCGGAGCA GGAGCAATTC CGGGGTCCGC TTGGACGGGT ACGCGCGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA TCCCGTCACG GGGCTGCTGT CAGCCAGCCA TGAGCAGAAG
GATGCCTGGG TGCGGGATAA CATCTACAGT ATCCTGGCCG TGTGGGGCCT GGGCATGGCC
TACCGTAAGA ATGCAGACCG CGATGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAAC
GTGGTGAAGC TGATGCGAGG TCTTCTCCAG TGCATGATGA GACAGGTGGC CAAAGTGGAG
AAGTTCAAAC ACACTCAGAG CACCAAGGAC AGCCTGCACG CCAAGTACAA CACCGCCACC
TGTGGCACGG TGGTGGGCGA CGACCAGTGG GGCCACCTCC AGGTGGATGC CACCTCTCTC
TTCCTCCTGT TCCTGGCCCA GATGACCGCC TCAGGCTTAC GTATCATTTT CACTCTCGAT
GAGGTGGCCT TCATACAGAA TCTTGTCTTT TACATAGAAG CTGCATATAA AGTCGCTGAT
TATGGAATGT GGGAGCGTGG AGATAAGACT AATCAGGGCA TCCCGGAATT GAATGCAAGC
TCCGTAGGAA TGGCCAAGGC AGCTCTTGAG GCAATTGATG AACTGGACCT TTTTGGAGCC
CATGGAGGAC GCAAGTCAGT GATTCATGTT CTGCCAGATG AGGTCGAGCA CTGCCAGTCT
ATTCTGTTCT CCATGCTGCC AAGAGCGTCG ACATCTAAAG AAATTGATGC TGGACTTCTT
TCCATTATTT CCTTCCCGGC CTTTGCAGTG GAAGATGTAA ACCTTGTAAA TGTGACCAAA
AATGAAATTA TTTCTAAGCT CCAGGGGCGT TATGGATGCT GTCGCTTCCT TCGAGATGGT
TATAAAACTC CAAGAGAGGA CCCTAATCGA CTGCATTATG ACCCTGCTGA ACTCAAGCTC
TTCGAAAACA TTGAATGTGA GTGGCCTGTG TTTTGGACAT ATTTTATAAT AGATGGAGTC
TTCAGTGGTG ATGCTGTTCA GGTCCAAGAA TACCGAGAGG CCCTGGAGGG AATACTCATC
AGAGGCAAGA ATGGGATCCG CCTGGTGCCT GAACTCTACG CTGTCCCGCC TAACAAGGTA
GATGAAGAGT ACAAGAATCC TCACACAGTA GACCGAGTTC CTATGGGGAA GGTGCCTCAT
CTGTGGGGCC AATCCTTGTA CATCCTCAGC TCGCTGTTGG CAGAGGGATT CCTTGCCGCT
GGTGAAATCG ATCCCTTAAA TAGAAGATTT TCCACTTCAG TCAAACCTGA TGTTGTAGTA
CAAGTTACTG TTTTGGCAGA AAACAATCAC ATTAAGGACT TATTGAGGAA ACACGGGGTG
AACGTCCAGA GTATCGCGGA CATTCATCCA ATTCAAGTCC AGCCGGGCCG GATTCTTAGT
CACATATATG CCAAGCTTGG ACGGAATAAG AATATGAATT TGAGTGGGCG ACCGTATCGA
CATATTGGTG TCCTTGGAAC CTCTAAACTA TATGTGATTA GGAACCAAAT CTTTACTTTT
ACACCCCAGT TCACCGACCA GCATCACTTC TACCTGGCCC TCGACAATGA GATGATCGTG
GAGATGCTAA GGATCGAGCT GGCCTACCTG TGCACCTGCT GGAGGATGAC GGGCAGACCC
ACACTCACCT TCCCCATCAG TCGCACCATG CTCACAAATG ATGGCTCAGA CATTCATTCT
GCTGTGCTCT CCACAATTAG AAAACTAGAG GATGGATATT TTGGAGGAGC CAGAGTAAAA
TTAGGGAACC TTTCGGAATT TCTCACCACA TCGTTCTACA CATATCTGAC TTTTCTGGAT
CCAGACTGTG ATGAGAAGTT GTTTGACAAT GCCAGCGAAG GGACTTTCAG TCCTGATAGT
GATTCAGATT TGGTAGGATA TCTGGAAGAC ACCTGTAATC AAGAAAGCCA AGACGAACTT
GACCATTATA TCAACCACCT TCTGCAAAGC ACATCGTTGA GGTCCTATCT GCCTCCTCTT
TGTAAGAACA CAGAAGACCG CCATGTCTTC AGTGCTATCC ACTCCACGCG GGACATACTT
TCTGTGATGG CAAAAGCAAA GGGTTTGGAA GTTCCATTTG TTCCCATGAC TTTGCCGACT
AAAGTTCTAA GTGCCCACCG TAAATCACTG AATCTTGTTG ATTCTCCTCA GCCACTCCTA
GAAAAGGTTC CTGAAAGTGA CTTTCAGTGG CCCAGAGATG ACCATGGTGA CGTGGACTGT
GAGAAGCTGG TTGAGCAGCT AAAAGATTGT TCGAACCTAC AGGACCAAGC AGACATTCTG
TACATTCTTT ATGTCATAAA GGGTCCCAGC TGGGACACAA ATCTCTCTGG ACAGCACGGG
GTCACCGTTC AAAACCTTCT TGGTGAGCTC TATGGGAAAG CCGGCTTGAA CCAGGAGTGG
GGTCTGATTC GCTACATCTC AGGCCTTCTC AGGAAGAAAG TGGAGGTCCT GGCTGAGGCC
TGCACAGACC TGCTTTCGCA CCAGAAGCAG CTCACCGTGG GCCTGCCGCC CGAGCCCCGG
GAGAAGATCA TCTCTGCGCC CCTTCCCCCA GAGGAGCTCA CAAAACTCAT CTACGAGGCC
AGTGGGCAGG ACATCAGCAT TGCCGTCCTC ACGCAGGAGA TTGTGGTTTA CCTGGCCATG
TATGTCAGGG CGCAGCCCAG CCTCTTTGTG GAGATGCTGA GACTCCGGAT TGGACTGATC
ATTCAGGTGA TGGCCACGGA GCTGGCACGG AGCCTGAACT GCTCAGGAGA AGAGGCTTCT
GAAAGTTTGA TGAACCTCAG CCCTTTCGAT ATGAAAAATC TCCTGCACCA TATTCTAAGT
GGGAAAGAGT TTGGCGTTGA AAGAAGTGTG CGCCCTATCC ACTCCTCCAC ATCCAGCCCT
ACCATCTCCA TCCACGAGGT GGGCCATACC GGAGTCACCA AAACTGAGAG GAGTGGCATT
AACAGACTGA GGAGTGAAAT GAAACAGATG ACTAGGCGGT TTAGTGCTGA TGAACAGTTC
TTTTCTGTGG GCCAGGCCGC GTCCAGCAGT GCGCATTCCT CCAAGTCTGC GGTAACAGTG
CCTCGGGATT ATTGTAGGTC CAGCACCCCA TCCTCGCCCA CTGGCACGTC ATCCTCAGAC
TCGGGAGGAC ATCACATCGG CTGGGGTGAG CGGCAGGGCC AGTGGCTGCG CAGGAGAAGG
CTGGATGGGG CCATCAACAG GGTCCCCGTG GGATTCTACC AGAGGGTGTG GAAGATCCTC
CAGAAGTGCC ACGGTCTCTC CATCGATGGT TATGTCCTCC CATCCTCGAC GACCCGAGAG
ATGACCCCGC ATGAGATCAA GTTTGCTGTC CATGTCGAAT CGGTGCTGAA CCGCGTGCCG
CAGCCCGAGT ACCGGCAGCT GCTGGTGGAA GCCATCATGG TGCTGACGCT GCTCTCGGAC
ACGGAGATGA CCAGCATCGG GGGCATCATC CACGTGGACC AGATCGTGCA GATGGCCAGT
CAGCTGTTCT TGCAGGACCA GGTGTCAATT GGTGCCATGG ACACCCTGGA GAAAGACCAA
GCCACAGGAA TCTGCCACTT CTTTTATGAC AGCGCTCCGA GTGGGGCTTA TGGGACGATG
ACCTACCTAA CAAGAGCAGT GGCTTCTTAT TTGCAGGAAT TGTTGCCCAA TTCGGGCTGC
CAGATGCAAT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006724559.1
CDS185..3916
Misc Feature(1)206..>1501(+)
Misc Feature(2)2588..2941(+)
Translation

Target ORF information:

RefSeq Version XM_006724496
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phosphorylase kinase, alpha 2 (liver) (PHKA2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006724496

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGCGGAGCA GGAGCAATTC CGGGGTCCGC TTGGACGGGT ACGCGCGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA TCCCGTCACG GGGCTGCTGT CAGCCAGCCA TGAGCAGAAG
GATGCCTGGG TGCGGGATAA CATCTACAGT ATCCTGGCCG TGTGGGGCCT GGGCATGGCC
TACCGTAAGA ATGCAGACCG CGATGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAAC
GTGGTGAAGC TGATGCGAGG TCTTCTCCAG TGCATGATGA GACAGGTGGC CAAAGTGGAG
AAGTTCAAAC ACACTCAGAG CACCAAGGAC AGCCTGCACG CCAAGTACAA CACCGCCACC
TGTGGCACGG TGGTGGGCGA CGACCAGTGG GGCCACCTCC AGGTGGATGC CACCTCTCTC
TTCCTCCTGT TCCTGGCCCA GATGACCGCC TCAGGCTTAC GTATCATTTT CACTCTCGAT
GAGGTGGCCT TCATACAGAA TCTTGTCTTT TACATAGAAG CTGCATATAA AGTCGCTGAT
TATGGAATGT GGGAGCGTGG AGATAAGACT AATCAGGGCA TCCCGGAATT GAATGCAAGC
TCCGTAGGAA TGGCCAAGGC AGCTCTTGAG GCAATTGATG AACTGGACCT TTTTGGAGCC
CATGGAGGAC GCAAGTCAGT GATTCATGTT CTGCCAGATG AGGTCGAGCA CTGCCAGTCT
ATTCTGTTCT CCATGCTGCC AAGAGCGTCG ACATCTAAAG AAATTGATGC TGGACTTCTT
TCCATTATTT CCTTCCCGGC CTTTGCAGTG GAAGATGTAA ACCTTGTAAA TGTGACCAAA
AATGAAATTA TTTCTAAGCT CCAGGGGCGT TATGGATGCT GTCGCTTCCT TCGAGATGGT
TATAAAACTC CAAGAGAGGA CCCTAATCGA CTGCATTATG ACCCTGCTGA ACTCAAGCTC
TTCGAAAACA TTGAATGTGA GTGGCCTGTG TTTTGGACAT ATTTTATAAT AGATGGAGTC
TTCAGTGGTG ATGCTGTTCA GGTCCAAGAA TACCGAGAGG CCCTGGAGGG AATACTCATC
AGAGGCAAGA ATGGGATCCG CCTGGTGCCT GAACTCTACG CTGTCCCGCC TAACAAGGTA
GATGAAGAGT ACAAGAATCC TCACACAGTA GACCGAGTTC CTATGGGGAA GGTGCCTCAT
CTGTGGGGCC AATCCTTGTA CATCCTCAGC TCGCTGTTGG CAGAGGGATT CCTTGCCGCT
GGTGAAATCG ATCCCTTAAA TAGAAGATTT TCCACTTCAG TCAAACCTGA TGTTGTAGTA
CAAGTTACTG TTTTGGCAGA AAACAATCAC ATTAAGGACT TATTGAGGAA ACACGGGGTG
AACGTCCAGA GTATCGCGGA CATTCATCCA ATTCAAGTCC AGCCGGGCCG GATTCTTAGT
CACATATATG CCAAGCTTGG ACGGAATAAG AATATGAATT TGAGTGGGCG ACCGTATCGA
CATATTGGTG TCCTTGGAAC CTCTAAACTA TATGTGATTA GGAACCAAAT CTTTACTTTT
ACACCCCAGT TCACCGACCA GCATCACTTC TACCTGGCCC TCGACAATGA GATGATCGTG
GAGATGCTAA GGATCGAGCT GGCCTACCTG TGCACCTGCT GGAGGATGAC GGGCAGACCC
ACACTCACCT TCCCCATCAG TCGCACCATG CTCACAAATG ATGGCTCAGA CATTCATTCT
GCTGTGCTCT CCACAATTAG AAAACTAGAG GATGGATATT TTGGAGGAGC CAGAGTAAAA
TTAGGGAACC TTTCGGAATT TCTCACCACA TCGTTCTACA CATATCTGAC TTTTCTGGAT
CCAGACTGTG ATGAGAAGTT GTTTGACAAT GCCAGCGAAG GGACTTTCAG TCCTGATAGT
GATTCAGATT TGGTAGGATA TCTGGAAGAC ACCTGTAATC AAGAAAGCCA AGACGAACTT
GACCATTATA TCAACCACCT TCTGCAAAGC ACATCGTTGA GGTCCTATCT GCCTCCTCTT
TGTAAGAACA CAGAAGACCG CCATGTCTTC AGTGCTATCC ACTCCACGCG GGACATACTT
TCTGTGATGG CAAAAGCAAA GGGTTTGGAA GTTCCATTTG TTCCCATGAC TTTGCCGACT
AAAGTTCTAA GTGCCCACCG TAAATCACTG AATCTTGTTG ATTCTCCTCA GCCACTCCTA
GAAAAGGTTC CTGAAAGTGA CTTTCAGTGG CCCAGAGATG ACCATGGTGA CGTGGACTGT
GAGAAGCTGG TTGAGCAGCT AAAAGATTGT TCGAACCTAC AGGACCAAGC AGACATTCTG
TACATTCTTT ATGTCATAAA GGGTCCCAGC TGGGACACAA ATCTCTCTGG ACAGCACGGG
GTCACCGTTC AAAACCTTCT TGGTGAGCTC TATGGGAAAG CCGGCTTGAA CCAGGAGTGG
GGTCTGATTC GCTACATCTC AGGCCTTCTC AGGAAGAAAG TGGAGGTCCT GGCTGAGGCC
TGCACAGACC TGCTTTCGCA CCAGAAGCAG CTCACCGTGG GCCTGCCGCC CGAGCCCCGG
GAGAAGATCA TCTCTGCGCC CCTTCCCCCA GAGGAGCTCA CAAAACTCAT CTACGAGGCC
AGTGGGCAGG ACATCAGCAT TGCCGTCCTC ACGCAGGAGA TTGTGGTTTA CCTGGCCATG
TATGTCAGGG CGCAGCCCAG CCTCTTTGTG GAGATGCTGA GACTCCGGAT TGGACTGATC
ATTCAGGTGA TGGCCACGGA GCTGGCACGG AGCCTGAACT GCTCAGGAGA AGAGGCTTCT
GAAAGTTTGA TGAACCTCAG CCCTTTCGAT ATGAAAAATC TCCTGCACCA TATTCTAAGT
GGGAAAGAGT TTGGCGTTGA AAGAAGTGTG CGCCCTATCC ACTCCTCCAC ATCCAGCCCT
ACCATCTCCA TCCACGAGGT GGGCCATACC GGAGTCACCA AAACTGAGAG GAGTGGCATT
AACAGACTGA GGAGTGAAAT GAAACAGATG ACTAGGCGGT TTAGTGCTGA TGAACAGTTC
TTTTCTGTGG GCCAGGCCGC GTCCAGCAGT GCGCATTCCT CCAAGTCTGC GGTAACAGTG
CCTCGGGATT ATTGTAGGTC CAGCACCCCA TCCTCGCCCA CTGGCACGTC ATCCTCAGAC
TCGGGAGGAC ATCACATCGG CTGGGGTGAG CGGCAGGGCC AGTGGCTGCG CAGGAGAAGG
CTGGATGGGG CCATCAACAG GGTCCCCGTG GGATTCTACC AGAGGGTGTG GAAGATCCTC
CAGAAGTGCC ACGGTCTCTC CATCGATGGT TATGTCCTCC CATCCTCGAC GACCCGAGAG
ATGACCCCGC ATGAGATCAA GTTTGCTGTC CATGTCGAAT CGGTGCTGAA CCGCGTGCCG
CAGCCCGAGT ACCGGCAGCT GCTGGTGGAA GCCATCATGG TGCTGACGCT GCTCTCGGAC
ACGGAGATGA CCAGCATCGG GGGCATCATC CACGTGGACC AGATCGTGCA GATGGCCAGT
CAGCTGTTCT TGCAGGACCA GGTGTCAATT GGTGCCATGG ACACCCTGGA GAAAGACCAA
GCCACAGGAA TCTGCCACTT CTTTTATGAC AGCGCTCCGA GTGGGGCTTA TGGGACGATG
ACCTACCTAA CAAGAGCAGT GGCTTCTTAT TTGCAGGAAT TGTTGCCCAA TTCGGGCTGC
CAGATGCAAT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu53931
Clone ID Related Accession (Same CDS sequence) XM_005274548
Accession Version XM_005274548.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3654bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167197.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578837917. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGCGGAGCA GGAGCAATTC CGGGGTCCGC TTGGACGGGT ACGCGCGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA TCCCGTCACG GGGCTGCTGT CAGCCAGCCA TGAGCAGAAG
GATGCCTGGG TGCGGGATAA CATCTACAGT ATCCTGGCCG TGTGGGGCCT GGGCATGGCC
TACCGTAAGA ATGCAGACCG CGATGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAAC
GTGGTGAAGC TGATGCGAGG TCTTCTCCAG TGCATGATGA GACAGGTGGC CAAAGTGGAG
AAGTTCAAAC ACACTCAGAG CACCAAGGAC AGCCTGCACG CCAAGTACAA CACCGCCACC
TGTGGCACGG TGGTGGGCGA CGACCAGTGG GGCCACCTCC AGGTGGATGC CACCTCTCTC
TTCCTCCTGT TCCTGGCCCA GATGACCGCC TCAGGCTTAC GTATCATTTT CACTCTCGAT
GAGGTGGCCT TCATACAGAA TCTTGTCTTT TACATAGAAG CTGCATATAA AGTCGCTGAT
TATGGAATGT GGGAGCGTGG AGATAAGACT AATCAGGGCA TCCCGGAATT GAATGCAAGC
TCCGTAGGAA TGGCCAAGGC AGCTCTTGAG GCAATTGATG AACTGGACCT TTTTGGAGCC
CATGGAGGAC GCAAGTCAGT GATTCATGTT CTGCCAGATG AGGTCGAGCA CTGCCAGTCT
ATTCTGTTCT CCATGCTGCC AAGAGCGTCG ACATCTAAAG AAATTGATGC TGGACTTCTT
TCCATTATTT CCTTCCCGGC CTTTGCAGTG GAAGATGTAA ACCTTGTAAA TGTGACCAAA
AATGAAATTA TTTCTAAGCT CCAGGGGCGT TATGGATGCT GTCGCTTCCT TCGAGATGGT
TATAAAACTC CAAGAGAGGA CCCTAATCGA CTGCATTATG ACCCTGCTGA ACTCAAGCTC
TTCGAAAACA TTGAATGTGA GTGGCCTGTG TTTTGGACAT ATTTTATAAT AGATGGAGTC
TTCAGTGGTG ATGCTGTTCA GGTCCAAGAA TACCGAGAGG CCCTGGAGGG AATACTCATC
AGAGGCAAGA ATGGGATCCG CCTGGTGCCT GAACTCTACG CTGTCCCGCC TAACAAGGTA
GATGAAGAGT ACAAGAATCC TCACACAGTA GACCGAGTTC CTATGGGGAA GGTGCCTCAT
CTGTGGGGCC AATCCTTGTA CATCCTCAGC TCGCTGTTGG CAGAGGGATT CCTTGCCGCT
GGTGAAATCG ATCCCTTAAA TAGAAGATTT TCCACTTCAG TCAAACCTGA TGTTGTAGTA
CAAGTTACTG TTTTGGCAGA AAACAATCAC ATTAAGGACT TATTGAGGAA ACACGGGGTG
AACGTCCAGA GTATCGCGGA CATTCATCCA ATTCAAGTCC AGCCGGGCCG GATTCTTAGT
CACATATATG CCAAGCTTGG ACGGAATAAG AATATGAATT TGAGTGGGCG ACCGTATCGA
CATATTGGTG TCCTTGGAAC CTCTAAACTA TATGTGATTA GGAACCAAAT CTTTACTTTT
ACACCCCAGT TCACCGACCA GCATCACTTC TACCTGGCCC TCGACAATGA GATGATCGTG
GAGATGCTAA GGATCGAGCT GGCCTACCTG TGCACCTGCT GGAGGATGAC GGGCAGACCC
ACACTCACCT TCCCCATCAG TCGCACCATG CTCACAAATG ATGGCTCAGA CATTCATTCT
GCTGTGCTCT CCACAATTAG AAAACTAGAG GATGGATATT TTGGAGGAGC CAGAGTAAAA
TTAGGGAACC TTTCGGAATT TCTCACCACA TCGTTCTACA CATATCTGAC TTTTCTGGAT
CCAGACTGTG ATGAGAAGTT GTTTGACAAT GCCAGCGAAG GGACTTTCAG TCCTGATAGT
GATTCAGATT TGGTAGGATA TCTGGAAGAC ACCTGTAATC AAGAAAGCCA AGACGAACTT
GACCATTATA TCAACCACCT TCTGCAAAGC ACATCGTTGA GGTCCTATCT GCCTCCTCTT
TGTAAGAACA CAGAAGACCG CCATGTCTTC AGTGCTATCC ACTCCACGCG GGACATACTT
TCTGTGATGG CAAAAGCAAA GGGTTTGGAA GTTCCATTTG TTCCCATGAC TTTGCCGACT
AAAGTTCTAA GTGCCCACCG TAAATCACTG AATCTTGTTG ATTCTCCTCA GCCACTCCTA
GAAAAGGTTC CTGAAAGTGA CTTTCAGTGG CCCAGAGATG ACCATGGTGA CGTGGACTGT
GAGAAGCTGG TTGAGCAGCT AAAAGATTGT TCGAACCTAC AGGACCAAGC AGACATTCTG
TACATTCTTT ATGTCATAAA GGGTCCCAGC TGGGACACAA ATCTCTCTGG ACAGCACGGG
GTCACCGTTC AAAACCTTCT TGGTGAGCTC TATGGGAAAG CCGGCTTGAA CCAGGAGTGG
GGTCTGATTC GCTACATCTC AGGCCTTCTC AGGAAGAAAG TGGAGGTCCT GGCTGAGGCC
TGCACAGACC TGCTTTCGCA CCAGAAGCAG CTCACCGTGG GCCTGCCGCC CGAGCCCCGG
GAGAAGATCA TCTCTGCGCC CCTTCCCCCA GAGGAGCTCA CAAAACTCAT CTACGAGGCC
AGTGGGCAGG ACATCAGCAT TGCCGTCCTC ACGCAGGAGA TTGTGGTTTA CCTGGCCATG
TATGTCAGGG CGCAGCCCAG CCTCTTTGTG GAGATGCTGA GACTCCGGAT TGGACTGATC
ATTCAGGTGA TGGCCACGGA GCTGGCACGG AGCCTGAACT GCTCAGGAGA AGAGGCTTCT
GAAAGTTTGA TGAACCTCAG CCCTTTCGAT ATGAAAAATC TCCTGCACCA TATTCTAAGT
GGGAAAGAGT TTGGCGTTGA AAGAAGTGTG CGCCCTATCC ACTCCTCCAC ATCCAGCCCT
ACCATCTCCA TCCACGAGGT GGGCCATACC GGAGTCACCA AAACTGAGAG GAGTGGCATT
AACAGACTGA GGAGTGAAAT GAAACAGATG ACTAGGCGGT TTAGTGCTGA TGAACAGAGG
TCCAGCACCC CATCCTCGCC CACTGGCACG TCATCCTCAG ACTCGGGAGG ACATCACATC
GGCTGGGGTG AGCGGCAGGG CCAGTGGCTG CGCAGGAGAA GGCTGGATGG GGCCATCAAC
AGGGTCCCCG TGGGATTCTA CCAGAGGGTG TGGAAGATCC TCCAGAAGTG CCACGGTCTC
TCCATCGATG GTTATGTCCT CCCATCCTCG ACGACCCGAG AGATGACCCC GCATGAGATC
AAGTTTGCTG TCCATGTCGA ATCGGTGCTG AACCGCGTGC CGCAGCCCGA GTACCGGCAG
CTGCTGGTGG AAGCCATCAT GGTGCTGACG CTGCTCTCGG ACACGGAGAT GACCAGCATC
GGGGGCATCA TCCACGTGGA CCAGATCGTG CAGATGGCCA GTCAGCTGTT CTTGCAGGAC
CAGGTGTCAA TTGGTGCCAT GGACACCCTG GAGAAAGACC AAGCCACAGG AATCTGCCAC
TTCTTTTATG ACAGCGCTCC GAGTGGGGCT TATGGGACGA TGACCTACCT AACAAGAGCA
GTGGCTTCTT ATTTGCAGGA ATTGTTGCCC AATTCGGGCT GCCAGATGCA ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005274605.1
CDS185..3838
Misc Feature(1)206..>1501(+)
Misc Feature(2)2588..2941(+)
Translation

Target ORF information:

RefSeq Version XM_005274548
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phosphorylase kinase, alpha 2 (liver) (PHKA2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005274548

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGCGGAGCA GGAGCAATTC CGGGGTCCGC TTGGACGGGT ACGCGCGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA TCCCGTCACG GGGCTGCTGT CAGCCAGCCA TGAGCAGAAG
GATGCCTGGG TGCGGGATAA CATCTACAGT ATCCTGGCCG TGTGGGGCCT GGGCATGGCC
TACCGTAAGA ATGCAGACCG CGATGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAAC
GTGGTGAAGC TGATGCGAGG TCTTCTCCAG TGCATGATGA GACAGGTGGC CAAAGTGGAG
AAGTTCAAAC ACACTCAGAG CACCAAGGAC AGCCTGCACG CCAAGTACAA CACCGCCACC
TGTGGCACGG TGGTGGGCGA CGACCAGTGG GGCCACCTCC AGGTGGATGC CACCTCTCTC
TTCCTCCTGT TCCTGGCCCA GATGACCGCC TCAGGCTTAC GTATCATTTT CACTCTCGAT
GAGGTGGCCT TCATACAGAA TCTTGTCTTT TACATAGAAG CTGCATATAA AGTCGCTGAT
TATGGAATGT GGGAGCGTGG AGATAAGACT AATCAGGGCA TCCCGGAATT GAATGCAAGC
TCCGTAGGAA TGGCCAAGGC AGCTCTTGAG GCAATTGATG AACTGGACCT TTTTGGAGCC
CATGGAGGAC GCAAGTCAGT GATTCATGTT CTGCCAGATG AGGTCGAGCA CTGCCAGTCT
ATTCTGTTCT CCATGCTGCC AAGAGCGTCG ACATCTAAAG AAATTGATGC TGGACTTCTT
TCCATTATTT CCTTCCCGGC CTTTGCAGTG GAAGATGTAA ACCTTGTAAA TGTGACCAAA
AATGAAATTA TTTCTAAGCT CCAGGGGCGT TATGGATGCT GTCGCTTCCT TCGAGATGGT
TATAAAACTC CAAGAGAGGA CCCTAATCGA CTGCATTATG ACCCTGCTGA ACTCAAGCTC
TTCGAAAACA TTGAATGTGA GTGGCCTGTG TTTTGGACAT ATTTTATAAT AGATGGAGTC
TTCAGTGGTG ATGCTGTTCA GGTCCAAGAA TACCGAGAGG CCCTGGAGGG AATACTCATC
AGAGGCAAGA ATGGGATCCG CCTGGTGCCT GAACTCTACG CTGTCCCGCC TAACAAGGTA
GATGAAGAGT ACAAGAATCC TCACACAGTA GACCGAGTTC CTATGGGGAA GGTGCCTCAT
CTGTGGGGCC AATCCTTGTA CATCCTCAGC TCGCTGTTGG CAGAGGGATT CCTTGCCGCT
GGTGAAATCG ATCCCTTAAA TAGAAGATTT TCCACTTCAG TCAAACCTGA TGTTGTAGTA
CAAGTTACTG TTTTGGCAGA AAACAATCAC ATTAAGGACT TATTGAGGAA ACACGGGGTG
AACGTCCAGA GTATCGCGGA CATTCATCCA ATTCAAGTCC AGCCGGGCCG GATTCTTAGT
CACATATATG CCAAGCTTGG ACGGAATAAG AATATGAATT TGAGTGGGCG ACCGTATCGA
CATATTGGTG TCCTTGGAAC CTCTAAACTA TATGTGATTA GGAACCAAAT CTTTACTTTT
ACACCCCAGT TCACCGACCA GCATCACTTC TACCTGGCCC TCGACAATGA GATGATCGTG
GAGATGCTAA GGATCGAGCT GGCCTACCTG TGCACCTGCT GGAGGATGAC GGGCAGACCC
ACACTCACCT TCCCCATCAG TCGCACCATG CTCACAAATG ATGGCTCAGA CATTCATTCT
GCTGTGCTCT CCACAATTAG AAAACTAGAG GATGGATATT TTGGAGGAGC CAGAGTAAAA
TTAGGGAACC TTTCGGAATT TCTCACCACA TCGTTCTACA CATATCTGAC TTTTCTGGAT
CCAGACTGTG ATGAGAAGTT GTTTGACAAT GCCAGCGAAG GGACTTTCAG TCCTGATAGT
GATTCAGATT TGGTAGGATA TCTGGAAGAC ACCTGTAATC AAGAAAGCCA AGACGAACTT
GACCATTATA TCAACCACCT TCTGCAAAGC ACATCGTTGA GGTCCTATCT GCCTCCTCTT
TGTAAGAACA CAGAAGACCG CCATGTCTTC AGTGCTATCC ACTCCACGCG GGACATACTT
TCTGTGATGG CAAAAGCAAA GGGTTTGGAA GTTCCATTTG TTCCCATGAC TTTGCCGACT
AAAGTTCTAA GTGCCCACCG TAAATCACTG AATCTTGTTG ATTCTCCTCA GCCACTCCTA
GAAAAGGTTC CTGAAAGTGA CTTTCAGTGG CCCAGAGATG ACCATGGTGA CGTGGACTGT
GAGAAGCTGG TTGAGCAGCT AAAAGATTGT TCGAACCTAC AGGACCAAGC AGACATTCTG
TACATTCTTT ATGTCATAAA GGGTCCCAGC TGGGACACAA ATCTCTCTGG ACAGCACGGG
GTCACCGTTC AAAACCTTCT TGGTGAGCTC TATGGGAAAG CCGGCTTGAA CCAGGAGTGG
GGTCTGATTC GCTACATCTC AGGCCTTCTC AGGAAGAAAG TGGAGGTCCT GGCTGAGGCC
TGCACAGACC TGCTTTCGCA CCAGAAGCAG CTCACCGTGG GCCTGCCGCC CGAGCCCCGG
GAGAAGATCA TCTCTGCGCC CCTTCCCCCA GAGGAGCTCA CAAAACTCAT CTACGAGGCC
AGTGGGCAGG ACATCAGCAT TGCCGTCCTC ACGCAGGAGA TTGTGGTTTA CCTGGCCATG
TATGTCAGGG CGCAGCCCAG CCTCTTTGTG GAGATGCTGA GACTCCGGAT TGGACTGATC
ATTCAGGTGA TGGCCACGGA GCTGGCACGG AGCCTGAACT GCTCAGGAGA AGAGGCTTCT
GAAAGTTTGA TGAACCTCAG CCCTTTCGAT ATGAAAAATC TCCTGCACCA TATTCTAAGT
GGGAAAGAGT TTGGCGTTGA AAGAAGTGTG CGCCCTATCC ACTCCTCCAC ATCCAGCCCT
ACCATCTCCA TCCACGAGGT GGGCCATACC GGAGTCACCA AAACTGAGAG GAGTGGCATT
AACAGACTGA GGAGTGAAAT GAAACAGATG ACTAGGCGGT TTAGTGCTGA TGAACAGAGG
TCCAGCACCC CATCCTCGCC CACTGGCACG TCATCCTCAG ACTCGGGAGG ACATCACATC
GGCTGGGGTG AGCGGCAGGG CCAGTGGCTG CGCAGGAGAA GGCTGGATGG GGCCATCAAC
AGGGTCCCCG TGGGATTCTA CCAGAGGGTG TGGAAGATCC TCCAGAAGTG CCACGGTCTC
TCCATCGATG GTTATGTCCT CCCATCCTCG ACGACCCGAG AGATGACCCC GCATGAGATC
AAGTTTGCTG TCCATGTCGA ATCGGTGCTG AACCGCGTGC CGCAGCCCGA GTACCGGCAG
CTGCTGGTGG AAGCCATCAT GGTGCTGACG CTGCTCTCGG ACACGGAGAT GACCAGCATC
GGGGGCATCA TCCACGTGGA CCAGATCGTG CAGATGGCCA GTCAGCTGTT CTTGCAGGAC
CAGGTGTCAA TTGGTGCCAT GGACACCCTG GAGAAAGACC AAGCCACAGG AATCTGCCAC
TTCTTTTATG ACAGCGCTCC GAGTGGGGCT TATGGGACGA TGACCTACCT AACAAGAGCA
GTGGCTTCTT ATTTGCAGGA ATTGTTGCCC AATTCGGGCT GCCAGATGCA ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59671
Clone ID Related Accession (Same CDS sequence) XM_011545537
Accession Version XM_011545537.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3633bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167197.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGCGGAGCA GGAGCAATTC CGGGGTCCGC TTGGACGGGT ACGCGCGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA TCCCGTCACG GGGCTGCTGT CAGCCAGCCA TGAGCAGAAG
GATGCCTGGG TGCGGGATAA CATCTACAGT ATCCTGGCCG TGTGGGGCCT GGGCATGGCC
TACCGTAAGA ATGCAGACCG CGATGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAAC
GTGGTGAAGC TGATGCGAGG TCTTCTCCAG TGCATGATGA GACAGGTGGC CAAAGTGGAG
AAGTTCAAAC ACACTCAGAG CACCAAGGAC AGCCTGCACG CCAAGTACAA CACCGCCACC
TGTGGCACGG TGGTGGGCGA CGACCAGTGG GGCCACCTCC AGGTGGATGC CACCTCTCTC
TTCCTCCTGT TCCTGGCCCA GATGACCGCC TCAGGCTTAC GTATCATTTT CACTCTCGAT
GAGGTGGCCT TCATACAGAA TCTTGTCTTT TACATAGAAG CTGCATATAA AGTCGCTGAT
TATGGAATGT GGGAGCGTGG AGATAAGACT AATCAGGGCA TCCCGGAATT GAATGCAAGC
TCCGTAGGAA TGGCCAAGTC TATTCTGTTC TCCATGCTGC CAAGAGCGTC GACATCTAAA
GAAATTGATG CTGGACTTCT TTCCATTATT TCCTTCCCGG CCTTTGCAGT GGAAGATGTA
AACCTTGTAA ATGTGACCAA AAATGAAATT ATTTCTAAGC TCCAGGGGCG TTATGGATGC
TGTCGCTTCC TTCGAGATGG TTATAAAACT CCAAGAGAGG ACCCTAATCG ACTGCATTAT
GACCCTGCTG AACTCAAGCT CTTCGAAAAC ATTGAATGTG AGTGGCCTGT GTTTTGGACA
TATTTTATAA TAGATGGAGT CTTCAGTGGT GATGCTGTTC AGGTCCAAGA ATACCGAGAG
GCCCTGGAGG GAATACTCAT CAGAGGCAAG AATGGGATCC GCCTGGTGCC TGAACTCTAC
GCTGTCCCGC CTAACAAGGT AGATGAAGAG TACAAGAATC CTCACACAGT AGACCGAGTT
CCTATGGGGA AGGTGCCTCA TCTGTGGGGC CAATCCTTGT ACATCCTCAG CTCGCTGTTG
GCAGAGGGAT TCCTTGCCGC TGGTGAAATC GATCCCTTAA ATAGAAGATT TTCCACTTCA
GTCAAACCTG ATGTTGTAGT ACAAGTTACT GTTTTGGCAG AAAACAATCA CATTAAGGAC
TTATTGAGGA AACACGGGGT GAACGTCCAG AGTATCGCGG ACATTCATCC AATTCAAGTC
CAGCCGGGCC GGATTCTTAG TCACATATAT GCCAAGCTTG GACGGAATAA GAATATGAAT
TTGAGTGGGC GACCGTATCG ACATATTGGT GTCCTTGGAA CCTCTAAACT ATATGTGATT
AGGAACCAAA TCTTTACTTT TACACCCCAG TTCACCGACC AGCATCACTT CTACCTGGCC
CTCGACAATG AGATGATCGT GGAGATGCTA AGGATCGAGC TGGCCTACCT GTGCACCTGC
TGGAGGATGA CGGGCAGACC CACACTCACC TTCCCCATCA GTCGCACCAT GCTCACAAAT
GATGGCTCAG ACATTCATTC TGCTGTGCTC TCCACAATTA GAAAACTAGA GGATGGATAT
TTTGGAGGAG CCAGAGTAAA ATTAGGGAAC CTTTCGGAAT TTCTCACCAC ATCGTTCTAC
ACATATCTGA CTTTTCTGGA TCCAGACTGT GATGAGAAGT TGTTTGACAA TGCCAGCGAA
GGGACTTTCA GTCCTGATAG TGATTCAGAT TTGGTAGGAT ATCTGGAAGA CACCTGTAAT
CAAGAAAGCC AAGACGAACT TGACCATTAT ATCAACCACC TTCTGCAAAG CACATCGTTG
AGGTCCTATC TGCCTCCTCT TTGTAAGAAC ACAGAAGACC GCCATGTCTT CAGTGCTATC
CACTCCACGC GGGACATACT TTCTGTGATG GCAAAAGCAA AGGGTTTGGA AGTTCCATTT
GTTCCCATGA CTTTGCCGAC TAAAGTTCTA AGTGCCCACC GTAAATCACT GAATCTTGTT
GATTCTCCTC AGCCACTCCT AGAAAAGGTT CCTGAAAGTG ACTTTCAGTG GCCCAGAGAT
GACCATGGTG ACGTGGACTG TGAGAAGCTG GTTGAGCAGC TAAAAGATTG TTCGAACCTA
CAGGACCAAG CAGACATTCT GTACATTCTT TATGTCATAA AGGGTCCCAG CTGGGACACA
AATCTCTCTG GACAGCACGG GGTCACCGTT CAAAACCTTC TTGGTGAGCT CTATGGGAAA
GCCGGCTTGA ACCAGGAGTG GGGTCTGATT CGCTACATCT CAGGCCTTCT CAGGAAGAAA
GTGGAGGTCC TGGCTGAGGC CTGCACAGAC CTGCTTTCGC ACCAGAAGCA GCTCACCGTG
GGCCTGCCGC CCGAGCCCCG GGAGAAGATC ATCTCTGCGC CCCTTCCCCC AGAGGAGCTC
ACAAAACTCA TCTACGAGGC CAGTGGGCAG GACATCAGCA TTGCCGTCCT CACGCAGGAG
ATTGTGGTTT ACCTGGCCAT GTATGTCAGG GCGCAGCCCA GCCTCTTTGT GGAGATGCTG
AGACTCCGGA TTGGACTGAT CATTCAGGTG ATGGCCACGG AGCTGGCACG GAGCCTGAAC
TGCTCAGGAG AAGAGGCTTC TGAAAGTTTG ATGAACCTCA GCCCTTTCGA TATGAAAAAT
CTCCTGCACC ATATTCTAAG TGGGAAAGAG TTTGGCGTTG AAAGAAGTGT GCGCCCTATC
CACTCCTCCA CATCCAGCCC TACCATCTCC ATCCACGAGG TGGGCCATAC CGGAGTCACC
AAAACTGAGA GGAGTGGCAT TAACAGACTG AGGAGTGAAA TGAAACAGAT GACTAGGCGG
TTTAGTGCTG ATGAACAGTT CTTTTCTGTG GGCCAGGCCG CGTCCAGCAG TGCGCATTCC
TCCAAGTCTG CGGTAACAGT GCCTCGGGAT TATTGTAGGT CCAGCACCCC ATCCTCGCCC
ACTGGCACGT CATCCTCAGA CTCGGGAGGA CATCACATCG GCTGGGGTGA GCGGCAGGGC
CAGTGGCTGC GCAGGAGAAG GCTGGATGGG GCCATCAACA GGGTCCCCGT GGGATTCTAC
CAGAGGGTGT GGAAGATCCT CCAGAAGTGC CACGGTCTCT CCATCGATGG TTATGTCCTC
CCATCCTCGA CGACCCGAGA GATGACCCCG CATGAGATCA AGTTTGCTGT CCATGTCGAA
TCGGTGCTGA ACCGCGTGCC GCAGCCCGAG TACCGGCAGC TGCTGGTGGA AGCCATCATG
GTGCTGACGC TGCTCTCGGA CACGGAGATG ACCAGCATCG GGGGCATCAT CCACGTGGAC
CAGATCGTGC AGATGGCCAG TCAGCTGTTC TTGCAGGACC AGGTGTCAAT TGGTGCCATG
GACACCCTGG AGAAAGACCA AGCCACAGGA ATCTGCCACT TCTTTTATGA CAGCGCTCCG
AGTGGGGCTT ATGGGACGAT GACCTACCTA ACAAGAGCAG TGGCTTCTTA TTTGCAGGAA
TTGTTGCCCA ATTCGGGCTG CCAGATGCAA TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011543839.1
CDS185..3817
Misc Feature(1)206..>1402(+)
Misc Feature(2)2489..2842(+)
Translation

Target ORF information:

RefSeq Version XM_011545537
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phosphorylase kinase, alpha 2 (liver) (PHKA2), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011545537

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGCGGAGCA GGAGCAATTC CGGGGTCCGC TTGGACGGGT ACGCGCGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA TCCCGTCACG GGGCTGCTGT CAGCCAGCCA TGAGCAGAAG
GATGCCTGGG TGCGGGATAA CATCTACAGT ATCCTGGCCG TGTGGGGCCT GGGCATGGCC
TACCGTAAGA ATGCAGACCG CGATGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAAC
GTGGTGAAGC TGATGCGAGG TCTTCTCCAG TGCATGATGA GACAGGTGGC CAAAGTGGAG
AAGTTCAAAC ACACTCAGAG CACCAAGGAC AGCCTGCACG CCAAGTACAA CACCGCCACC
TGTGGCACGG TGGTGGGCGA CGACCAGTGG GGCCACCTCC AGGTGGATGC CACCTCTCTC
TTCCTCCTGT TCCTGGCCCA GATGACCGCC TCAGGCTTAC GTATCATTTT CACTCTCGAT
GAGGTGGCCT TCATACAGAA TCTTGTCTTT TACATAGAAG CTGCATATAA AGTCGCTGAT
TATGGAATGT GGGAGCGTGG AGATAAGACT AATCAGGGCA TCCCGGAATT GAATGCAAGC
TCCGTAGGAA TGGCCAAGTC TATTCTGTTC TCCATGCTGC CAAGAGCGTC GACATCTAAA
GAAATTGATG CTGGACTTCT TTCCATTATT TCCTTCCCGG CCTTTGCAGT GGAAGATGTA
AACCTTGTAA ATGTGACCAA AAATGAAATT ATTTCTAAGC TCCAGGGGCG TTATGGATGC
TGTCGCTTCC TTCGAGATGG TTATAAAACT CCAAGAGAGG ACCCTAATCG ACTGCATTAT
GACCCTGCTG AACTCAAGCT CTTCGAAAAC ATTGAATGTG AGTGGCCTGT GTTTTGGACA
TATTTTATAA TAGATGGAGT CTTCAGTGGT GATGCTGTTC AGGTCCAAGA ATACCGAGAG
GCCCTGGAGG GAATACTCAT CAGAGGCAAG AATGGGATCC GCCTGGTGCC TGAACTCTAC
GCTGTCCCGC CTAACAAGGT AGATGAAGAG TACAAGAATC CTCACACAGT AGACCGAGTT
CCTATGGGGA AGGTGCCTCA TCTGTGGGGC CAATCCTTGT ACATCCTCAG CTCGCTGTTG
GCAGAGGGAT TCCTTGCCGC TGGTGAAATC GATCCCTTAA ATAGAAGATT TTCCACTTCA
GTCAAACCTG ATGTTGTAGT ACAAGTTACT GTTTTGGCAG AAAACAATCA CATTAAGGAC
TTATTGAGGA AACACGGGGT GAACGTCCAG AGTATCGCGG ACATTCATCC AATTCAAGTC
CAGCCGGGCC GGATTCTTAG TCACATATAT GCCAAGCTTG GACGGAATAA GAATATGAAT
TTGAGTGGGC GACCGTATCG ACATATTGGT GTCCTTGGAA CCTCTAAACT ATATGTGATT
AGGAACCAAA TCTTTACTTT TACACCCCAG TTCACCGACC AGCATCACTT CTACCTGGCC
CTCGACAATG AGATGATCGT GGAGATGCTA AGGATCGAGC TGGCCTACCT GTGCACCTGC
TGGAGGATGA CGGGCAGACC CACACTCACC TTCCCCATCA GTCGCACCAT GCTCACAAAT
GATGGCTCAG ACATTCATTC TGCTGTGCTC TCCACAATTA GAAAACTAGA GGATGGATAT
TTTGGAGGAG CCAGAGTAAA ATTAGGGAAC CTTTCGGAAT TTCTCACCAC ATCGTTCTAC
ACATATCTGA CTTTTCTGGA TCCAGACTGT GATGAGAAGT TGTTTGACAA TGCCAGCGAA
GGGACTTTCA GTCCTGATAG TGATTCAGAT TTGGTAGGAT ATCTGGAAGA CACCTGTAAT
CAAGAAAGCC AAGACGAACT TGACCATTAT ATCAACCACC TTCTGCAAAG CACATCGTTG
AGGTCCTATC TGCCTCCTCT TTGTAAGAAC ACAGAAGACC GCCATGTCTT CAGTGCTATC
CACTCCACGC GGGACATACT TTCTGTGATG GCAAAAGCAA AGGGTTTGGA AGTTCCATTT
GTTCCCATGA CTTTGCCGAC TAAAGTTCTA AGTGCCCACC GTAAATCACT GAATCTTGTT
GATTCTCCTC AGCCACTCCT AGAAAAGGTT CCTGAAAGTG ACTTTCAGTG GCCCAGAGAT
GACCATGGTG ACGTGGACTG TGAGAAGCTG GTTGAGCAGC TAAAAGATTG TTCGAACCTA
CAGGACCAAG CAGACATTCT GTACATTCTT TATGTCATAA AGGGTCCCAG CTGGGACACA
AATCTCTCTG GACAGCACGG GGTCACCGTT CAAAACCTTC TTGGTGAGCT CTATGGGAAA
GCCGGCTTGA ACCAGGAGTG GGGTCTGATT CGCTACATCT CAGGCCTTCT CAGGAAGAAA
GTGGAGGTCC TGGCTGAGGC CTGCACAGAC CTGCTTTCGC ACCAGAAGCA GCTCACCGTG
GGCCTGCCGC CCGAGCCCCG GGAGAAGATC ATCTCTGCGC CCCTTCCCCC AGAGGAGCTC
ACAAAACTCA TCTACGAGGC CAGTGGGCAG GACATCAGCA TTGCCGTCCT CACGCAGGAG
ATTGTGGTTT ACCTGGCCAT GTATGTCAGG GCGCAGCCCA GCCTCTTTGT GGAGATGCTG
AGACTCCGGA TTGGACTGAT CATTCAGGTG ATGGCCACGG AGCTGGCACG GAGCCTGAAC
TGCTCAGGAG AAGAGGCTTC TGAAAGTTTG ATGAACCTCA GCCCTTTCGA TATGAAAAAT
CTCCTGCACC ATATTCTAAG TGGGAAAGAG TTTGGCGTTG AAAGAAGTGT GCGCCCTATC
CACTCCTCCA CATCCAGCCC TACCATCTCC ATCCACGAGG TGGGCCATAC CGGAGTCACC
AAAACTGAGA GGAGTGGCAT TAACAGACTG AGGAGTGAAA TGAAACAGAT GACTAGGCGG
TTTAGTGCTG ATGAACAGTT CTTTTCTGTG GGCCAGGCCG CGTCCAGCAG TGCGCATTCC
TCCAAGTCTG CGGTAACAGT GCCTCGGGAT TATTGTAGGT CCAGCACCCC ATCCTCGCCC
ACTGGCACGT CATCCTCAGA CTCGGGAGGA CATCACATCG GCTGGGGTGA GCGGCAGGGC
CAGTGGCTGC GCAGGAGAAG GCTGGATGGG GCCATCAACA GGGTCCCCGT GGGATTCTAC
CAGAGGGTGT GGAAGATCCT CCAGAAGTGC CACGGTCTCT CCATCGATGG TTATGTCCTC
CCATCCTCGA CGACCCGAGA GATGACCCCG CATGAGATCA AGTTTGCTGT CCATGTCGAA
TCGGTGCTGA ACCGCGTGCC GCAGCCCGAG TACCGGCAGC TGCTGGTGGA AGCCATCATG
GTGCTGACGC TGCTCTCGGA CACGGAGATG ACCAGCATCG GGGGCATCAT CCACGTGGAC
CAGATCGTGC AGATGGCCAG TCAGCTGTTC TTGCAGGACC AGGTGTCAAT TGGTGCCATG
GACACCCTGG AGAAAGACCA AGCCACAGGA ATCTGCCACT TCTTTTATGA CAGCGCTCCG
AGTGGGGCTT ATGGGACGAT GACCTACCTA ACAAGAGCAG TGGCTTCTTA TTTGCAGGAA
TTGTTGCCCA ATTCGGGCTG CCAGATGCAA TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu53932
Clone ID Related Accession (Same CDS sequence) XM_005274550
Accession Version XM_005274550.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3624bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167197.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578837918. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGCGGAGCA GGAGCAATTC CGGGGTCCGC TTGGACGGGT ACGCGCGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA TCCCGTCACG GGGCTGCTGT CAGCCAGCCA TGAGCAGAAG
GATGCCTGGG TGCGGGATAA CATCTACAGT ATCCTGGCCG TGTGGGGCCT GGGCATGGCC
TACCGTAAGA ATGCAGACCG CGATGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAAC
GTGGTGAAGC TGATGCGAGG TCTTCTCCAG TGCATGATGA GACAGGTGGC CAAAGTGGAG
AAGTTCAAAC ACACTCAGAG CACCAAGGAC AGCCTGCACG CCAAGTACAA CACCGCCACC
TGTGGCACGG TGGTGGGCGA CGACCAGTGG GGCCACCTCC AGGTGGATGC CACCTCTCTC
TTCCTCCTGT TCCTGGCCCA GATGACCGCC TCAGGCTTAC GTATCATTTT CACTCTCGAT
GAGGTGGCCT TCATACAGAA TCTTGTCTTT TACATAGAAG CTGCATATAA AGTCGCTGAT
TATGGAATGT GGGAGCGTGG AGATAAGACT AATCAGGGCA TCCCGGAATT GAATGCAAGC
TCCGTAGGAA TGGCCAAGGC AGCTCTTGAG GCAATTGATG AACTGGACCT TTTTGGAGCC
CATGGAGGAC GCAAGTCAGT GATTCATGTT CTGCCAGATG AGGTCGAGCA CTGCCAGTCT
ATTCTGTTCT CCATGCTGCC AAGAGCGTCG ACATCTAAAG AAATTGATGC TGGACTTCTT
TCCATTATTT CCTTCCCGGC CTTTGCAGTG GAAGATGTAA ACCTTGTAAA TGTGACCAAA
AATGAAATTA TTTCTAAGCT CCAGGGGCGT TATGGATGCT GTCGCTTCCT TCGAGATGGT
TATAAAACTC CAAGAGAGGA CCCTAATCGA CTGCATTATG ACCCTGCTGA ACTCAAGCTC
TTCGAAAACA TTGAATGTGA GTGGCCTGTG TTTTGGACAT ATTTTATAAT AGATGGAGTC
TTCAGTGGTG ATGCTGTTCA GGTCCAAGAA TACCGAGAGG CCCTGGAGGG AATACTCATC
AGAGGCAAGA ATGGGATCCG CCTGGTGCCT GAACTCTACG CTGTCCCGCC TAACAAGGTA
GATGAAGAGT ACAAGAATCC TCACACAGTA GACCGAGTTC CTATGGGGAA GGTGCCTCAT
CTGTGGGGCC AATCCTTGTA CATCCTCAGC TCGCTGTTGG CAGAGGGATT CCTTGCCGCT
GGTGAAATCG ATCCCTTAAA TAGAAGATTT TCCACTTCAG TCAAACCTGA TGTTGTAGTA
CAAGTTACTG TTTTGGCAGA AAACAATCAC ATTAAGGACT TATTGAGGAA ACACGGGGTG
AACGTCCAGA GTATCGCGGA CATTCATCCA ATTCAAGTCC AGCCGGGCCG GATTCTTAGT
CACATATATG CCAAGCTTGG ACGGAATAAG AATATGAATT TGAGTGGGCG ACCGTATCGA
CATATTGGTG TCCTTGGAAC CTCTAAACTA TATGTGATTA GGAACCAAAT CTTTACTTTT
ACACCCCAGT TCACCGACCA GCATCACTTC TACCTGGCCC TCGACAATGA GATGATCGTG
GAGATGCTAA GGATCGAGCT GGCCTACCTG TGCACCTGCT GGAGGATGAC GGGCAGACCC
ACACTCACCT TCCCCATCAG TCGCACCATG CTCACAAATG ATGGCTCAGA CATTCATTCT
GCTGTGCTCT CCACAATTAG AAAACTAGAG GATGGATATT TTGGAGGAGC CAGAGTAAAA
TTAGGGAACC TTTCGGAATT TCTCACCACA TCGTTCTACA CATATCTGAC TTTTCTGGAT
CCAGACTGTG ATGAGAAGTT GTTTGACAAT GCCAGCGAAG GGACTTTCAG TCCTGATAGT
GATTCAGATT TGGTAGGATA TCTGGAAGAC ACCTGTAATC AAGAAAGCCA AGACGAACTT
GACCATTATA TCAACCACCT TCTGCAAAGC ACATCGTTGA GGTCCTATCT GCCTCCTCTT
TGTAAGAACA CAGAAGACCG CCATGTCTTC AGTGCTATCC ACTCCACGCG GGACATACTT
TCTGTGATGG CAAAAGCAAA GGGTTTGGAA GTTCCATTTG TTCCCATGAC TTTGCCGACT
AAAGTTCTAA GTGCCCACCG TAAATCACTG AATCTTGTTG ATTCTCCTCA GCCACTCCTA
GAAAAGGTTC CTGAAAGTGA CTTTCAGTGG CCCAGAGATG ACCATGGTGA CGTGGACTGT
GAGAAGCTGG TTGAGCAGCT AAAAGATTGT TCGAACCTAC AGGACCAAGC AGACATTCTG
TACATTCTTT ATGTCATAAA GGGTCCCAGC TGGGACACAA ATCTCTCTGG ACAGCACGGG
GTCACCGTTC AAAACCTTCT TGGTGAGCTC TATGGGAAAG CCGGCTTGAA CCAGGAGTGG
GGTCTGATTC GCTACATCTC AGGCCTTCTC AGGAAGAAAG TGGAGGTCCT GGCTGAGGCC
TGCACAGACC TGCTTTCGCA CCAGAAGCAG CTCACCGTGG GCCTGCCGCC CGAGCCCCGG
GAGAAGATCA TCTCTGCGCC CCTTCCCCCA GAGGAGCTCA CAAAACTCAT CTACGAGGCC
AGTGGGCAGG ACATCAGCAT TGCCGTCCTC ACGCAGGAGA TTGTGGTTTA CCTGGCCATG
TATGTCAGGG CGCAGCCCAG CCTCTTTGTG GAGATGCTGA GACTCCGGAT TGGACTGATC
ATTCAGGTGA TGGCCACGGA GCTGGCACGG AGCCTGAACT GCTCAGGAGA AGAGGCTTCT
GAAAGTTTGA TGAACCTCAG CCCTTTCGAT ATGAAAAATC TCCTGCACCA TATTCTAAGT
GGGAAAGAGT TTGGCGTTGA AAGAAGTGTG CGCCCTATCC ACTCCTCCAC ATCCAGCCCT
ACCATCTCCA TCCACGAGGT GGGCCATACC GGAGTCACCA AAACTGAGAG GAGTGGCATT
AACAGACTGA GGAGTGAAAT GAAACAGAGG TCCAGCACCC CATCCTCGCC CACTGGCACG
TCATCCTCAG ACTCGGGAGG ACATCACATC GGCTGGGGTG AGCGGCAGGG CCAGTGGCTG
CGCAGGAGAA GGCTGGATGG GGCCATCAAC AGGGTCCCCG TGGGATTCTA CCAGAGGGTG
TGGAAGATCC TCCAGAAGTG CCACGGTCTC TCCATCGATG GTTATGTCCT CCCATCCTCG
ACGACCCGAG AGATGACCCC GCATGAGATC AAGTTTGCTG TCCATGTCGA ATCGGTGCTG
AACCGCGTGC CGCAGCCCGA GTACCGGCAG CTGCTGGTGG AAGCCATCAT GGTGCTGACG
CTGCTCTCGG ACACGGAGAT GACCAGCATC GGGGGCATCA TCCACGTGGA CCAGATCGTG
CAGATGGCCA GTCAGCTGTT CTTGCAGGAC CAGGTGTCAA TTGGTGCCAT GGACACCCTG
GAGAAAGACC AAGCCACAGG AATCTGCCAC TTCTTTTATG ACAGCGCTCC GAGTGGGGCT
TATGGGACGA TGACCTACCT AACAAGAGCA GTGGCTTCTT ATTTGCAGGA ATTGTTGCCC
AATTCGGGCT GCCAGATGCA ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005274607.1
CDS185..3808
Misc Feature(1)206..>1501(+)
Misc Feature(2)2588..2941(+)
Translation

Target ORF information:

RefSeq Version XM_005274550
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phosphorylase kinase, alpha 2 (liver) (PHKA2), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005274550

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGCGGAGCA GGAGCAATTC CGGGGTCCGC TTGGACGGGT ACGCGCGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA TCCCGTCACG GGGCTGCTGT CAGCCAGCCA TGAGCAGAAG
GATGCCTGGG TGCGGGATAA CATCTACAGT ATCCTGGCCG TGTGGGGCCT GGGCATGGCC
TACCGTAAGA ATGCAGACCG CGATGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAAC
GTGGTGAAGC TGATGCGAGG TCTTCTCCAG TGCATGATGA GACAGGTGGC CAAAGTGGAG
AAGTTCAAAC ACACTCAGAG CACCAAGGAC AGCCTGCACG CCAAGTACAA CACCGCCACC
TGTGGCACGG TGGTGGGCGA CGACCAGTGG GGCCACCTCC AGGTGGATGC CACCTCTCTC
TTCCTCCTGT TCCTGGCCCA GATGACCGCC TCAGGCTTAC GTATCATTTT CACTCTCGAT
GAGGTGGCCT TCATACAGAA TCTTGTCTTT TACATAGAAG CTGCATATAA AGTCGCTGAT
TATGGAATGT GGGAGCGTGG AGATAAGACT AATCAGGGCA TCCCGGAATT GAATGCAAGC
TCCGTAGGAA TGGCCAAGGC AGCTCTTGAG GCAATTGATG AACTGGACCT TTTTGGAGCC
CATGGAGGAC GCAAGTCAGT GATTCATGTT CTGCCAGATG AGGTCGAGCA CTGCCAGTCT
ATTCTGTTCT CCATGCTGCC AAGAGCGTCG ACATCTAAAG AAATTGATGC TGGACTTCTT
TCCATTATTT CCTTCCCGGC CTTTGCAGTG GAAGATGTAA ACCTTGTAAA TGTGACCAAA
AATGAAATTA TTTCTAAGCT CCAGGGGCGT TATGGATGCT GTCGCTTCCT TCGAGATGGT
TATAAAACTC CAAGAGAGGA CCCTAATCGA CTGCATTATG ACCCTGCTGA ACTCAAGCTC
TTCGAAAACA TTGAATGTGA GTGGCCTGTG TTTTGGACAT ATTTTATAAT AGATGGAGTC
TTCAGTGGTG ATGCTGTTCA GGTCCAAGAA TACCGAGAGG CCCTGGAGGG AATACTCATC
AGAGGCAAGA ATGGGATCCG CCTGGTGCCT GAACTCTACG CTGTCCCGCC TAACAAGGTA
GATGAAGAGT ACAAGAATCC TCACACAGTA GACCGAGTTC CTATGGGGAA GGTGCCTCAT
CTGTGGGGCC AATCCTTGTA CATCCTCAGC TCGCTGTTGG CAGAGGGATT CCTTGCCGCT
GGTGAAATCG ATCCCTTAAA TAGAAGATTT TCCACTTCAG TCAAACCTGA TGTTGTAGTA
CAAGTTACTG TTTTGGCAGA AAACAATCAC ATTAAGGACT TATTGAGGAA ACACGGGGTG
AACGTCCAGA GTATCGCGGA CATTCATCCA ATTCAAGTCC AGCCGGGCCG GATTCTTAGT
CACATATATG CCAAGCTTGG ACGGAATAAG AATATGAATT TGAGTGGGCG ACCGTATCGA
CATATTGGTG TCCTTGGAAC CTCTAAACTA TATGTGATTA GGAACCAAAT CTTTACTTTT
ACACCCCAGT TCACCGACCA GCATCACTTC TACCTGGCCC TCGACAATGA GATGATCGTG
GAGATGCTAA GGATCGAGCT GGCCTACCTG TGCACCTGCT GGAGGATGAC GGGCAGACCC
ACACTCACCT TCCCCATCAG TCGCACCATG CTCACAAATG ATGGCTCAGA CATTCATTCT
GCTGTGCTCT CCACAATTAG AAAACTAGAG GATGGATATT TTGGAGGAGC CAGAGTAAAA
TTAGGGAACC TTTCGGAATT TCTCACCACA TCGTTCTACA CATATCTGAC TTTTCTGGAT
CCAGACTGTG ATGAGAAGTT GTTTGACAAT GCCAGCGAAG GGACTTTCAG TCCTGATAGT
GATTCAGATT TGGTAGGATA TCTGGAAGAC ACCTGTAATC AAGAAAGCCA AGACGAACTT
GACCATTATA TCAACCACCT TCTGCAAAGC ACATCGTTGA GGTCCTATCT GCCTCCTCTT
TGTAAGAACA CAGAAGACCG CCATGTCTTC AGTGCTATCC ACTCCACGCG GGACATACTT
TCTGTGATGG CAAAAGCAAA GGGTTTGGAA GTTCCATTTG TTCCCATGAC TTTGCCGACT
AAAGTTCTAA GTGCCCACCG TAAATCACTG AATCTTGTTG ATTCTCCTCA GCCACTCCTA
GAAAAGGTTC CTGAAAGTGA CTTTCAGTGG CCCAGAGATG ACCATGGTGA CGTGGACTGT
GAGAAGCTGG TTGAGCAGCT AAAAGATTGT TCGAACCTAC AGGACCAAGC AGACATTCTG
TACATTCTTT ATGTCATAAA GGGTCCCAGC TGGGACACAA ATCTCTCTGG ACAGCACGGG
GTCACCGTTC AAAACCTTCT TGGTGAGCTC TATGGGAAAG CCGGCTTGAA CCAGGAGTGG
GGTCTGATTC GCTACATCTC AGGCCTTCTC AGGAAGAAAG TGGAGGTCCT GGCTGAGGCC
TGCACAGACC TGCTTTCGCA CCAGAAGCAG CTCACCGTGG GCCTGCCGCC CGAGCCCCGG
GAGAAGATCA TCTCTGCGCC CCTTCCCCCA GAGGAGCTCA CAAAACTCAT CTACGAGGCC
AGTGGGCAGG ACATCAGCAT TGCCGTCCTC ACGCAGGAGA TTGTGGTTTA CCTGGCCATG
TATGTCAGGG CGCAGCCCAG CCTCTTTGTG GAGATGCTGA GACTCCGGAT TGGACTGATC
ATTCAGGTGA TGGCCACGGA GCTGGCACGG AGCCTGAACT GCTCAGGAGA AGAGGCTTCT
GAAAGTTTGA TGAACCTCAG CCCTTTCGAT ATGAAAAATC TCCTGCACCA TATTCTAAGT
GGGAAAGAGT TTGGCGTTGA AAGAAGTGTG CGCCCTATCC ACTCCTCCAC ATCCAGCCCT
ACCATCTCCA TCCACGAGGT GGGCCATACC GGAGTCACCA AAACTGAGAG GAGTGGCATT
AACAGACTGA GGAGTGAAAT GAAACAGAGG TCCAGCACCC CATCCTCGCC CACTGGCACG
TCATCCTCAG ACTCGGGAGG ACATCACATC GGCTGGGGTG AGCGGCAGGG CCAGTGGCTG
CGCAGGAGAA GGCTGGATGG GGCCATCAAC AGGGTCCCCG TGGGATTCTA CCAGAGGGTG
TGGAAGATCC TCCAGAAGTG CCACGGTCTC TCCATCGATG GTTATGTCCT CCCATCCTCG
ACGACCCGAG AGATGACCCC GCATGAGATC AAGTTTGCTG TCCATGTCGA ATCGGTGCTG
AACCGCGTGC CGCAGCCCGA GTACCGGCAG CTGCTGGTGG AAGCCATCAT GGTGCTGACG
CTGCTCTCGG ACACGGAGAT GACCAGCATC GGGGGCATCA TCCACGTGGA CCAGATCGTG
CAGATGGCCA GTCAGCTGTT CTTGCAGGAC CAGGTGTCAA TTGGTGCCAT GGACACCCTG
GAGAAAGACC AAGCCACAGG AATCTGCCAC TTCTTTTATG ACAGCGCTCC GAGTGGGGCT
TATGGGACGA TGACCTACCT AACAAGAGCA GTGGCTTCTT ATTTGCAGGA ATTGTTGCCC
AATTCGGGCT GCCAGATGCA ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu53933
Clone ID Related Accession (Same CDS sequence) XM_006724498
Accession Version XM_006724498.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3186bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167197.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578837919. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGTGGGAGC GTGGAGATAA GACTAATCAG GGCATCCCGG AATTGAATGC AAGCTCCGTA 
GGAATGGCCA AGGCAGCTCT TGAGGCAATT GATGAACTGG ACCTTTTTGG AGCCCATGGA
GGACGCAAGT CAGTGATTCA TGTTCTGCCA GATGAGGTCG AGCACTGCCA GTCTATTCTG
TTCTCCATGC TGCCAAGAGC GTCGACATCT AAAGAAATTG ATGCTGGACT TCTTTCCATT
ATTTCCTTCC CGGCCTTTGC AGTGGAAGAT GTAAACCTTG TAAATGTGAC CAAAAATGAA
ATTATTTCTA AGCTCCAGGG GCGTTATGGA TGCTGTCGCT TCCTTCGAGA TGGTTATAAA
ACTCCAAGAG AGGACCCTAA TCGACTGCAT TATGACCCTG CTGAACTCAA GCTCTTCGAA
AACATTGAAT GTGAGTGGCC TGTGTTTTGG ACATATTTTA TAATAGATGG AGTCTTCAGT
GGTGATGCTG TTCAGGTCCA AGAATACCGA GAGGCCCTGG AGGGAATACT CATCAGAGGC
AAGAATGGGA TCCGCCTGGT GCCTGAACTC TACGCTGTCC CGCCTAACAA GGTAGATGAA
GAGTACAAGA ATCCTCACAC AGTAGACCGA GTTCCTATGG GGAAGGTGCC TCATCTGTGG
GGCCAATCCT TGTACATCCT CAGCTCGCTG TTGGCAGAGG GATTCCTTGC CGCTGGTGAA
ATCGATCCCT TAAATAGAAG ATTTTCCACT TCAGTCAAAC CTGATGTTGT AGTACAAGTT
ACTGTTTTGG CAGAAAACAA TCACATTAAG GACTTATTGA GGAAACACGG GGTGAACGTC
CAGAGTATCG CGGACATTCA TCCAATTCAA GTCCAGCCGG GCCGGATTCT TAGTCACATA
TATGCCAAGC TTGGACGGAA TAAGAATATG AATTTGAGTG GGCGACCGTA TCGACATATT
GGTGTCCTTG GAACCTCTAA ACTATATGTG ATTAGGAACC AAATCTTTAC TTTTACACCC
CAGTTCACCG ACCAGCATCA CTTCTACCTG GCCCTCGACA ATGAGATGAT CGTGGAGATG
CTAAGGATCG AGCTGGCCTA CCTGTGCACC TGCTGGAGGA TGACGGGCAG ACCCACACTC
ACCTTCCCCA TCAGTCGCAC CATGCTCACA AATGATGGCT CAGACATTCA TTCTGCTGTG
CTCTCCACAA TTAGAAAACT AGAGGATGGA TATTTTGGAG GAGCCAGAGT AAAATTAGGG
AACCTTTCGG AATTTCTCAC CACATCGTTC TACACATATC TGACTTTTCT GGATCCAGAC
TGTGATGAGA AGTTGTTTGA CAATGCCAGC GAAGGGACTT TCAGTCCTGA TAGTGATTCA
GATTTGGTAG GATATCTGGA AGACACCTGT AATCAAGAAA GCCAAGACGA ACTTGACCAT
TATATCAACC ACCTTCTGCA AAGCACATCG TTGAGGTCCT ATCTGCCTCC TCTTTGTAAG
AACACAGAAG ACCGCCATGT CTTCAGTGCT ATCCACTCCA CGCGGGACAT ACTTTCTGTG
ATGGCAAAAG CAAAGGGTTT GGAAGTTCCA TTTGTTCCCA TGACTTTGCC GACTAAAGTT
CTAAGTGCCC ACCGTAAATC ACTGAATCTT GTTGATTCTC CTCAGCCACT CCTAGAAAAG
GTTCCTGAAA GTGACTTTCA GTGGCCCAGA GATGACCATG GTGACGTGGA CTGTGAGAAG
CTGGTTGAGC AGCTAAAAGA TTGTTCGAAC CTACAGGACC AAGCAGACAT TCTGTACATT
CTTTATGTCA TAAAGGGTCC CAGCTGGGAC ACAAATCTCT CTGGACAGCA CGGGGTCACC
GTTCAAAACC TTCTTGGTGA GCTCTATGGG AAAGCCGGCT TGAACCAGGA GTGGGGTCTG
ATTCGCTACA TCTCAGGCCT TCTCAGGAAG AAAGTGGAGG TCCTGGCTGA GGCCTGCACA
GACCTGCTTT CGCACCAGAA GCAGCTCACC GTGGGCCTGC CGCCCGAGCC CCGGGAGAAG
ATCATCTCTG CGCCCCTTCC CCCAGAGGAG CTCACAAAAC TCATCTACGA GGCCAGTGGG
CAGGACATCA GCATTGCCGT CCTCACGCAG GAGATTGTGG TTTACCTGGC CATGTATGTC
AGGGCGCAGC CCAGCCTCTT TGTGGAGATG CTGAGACTCC GGATTGGACT GATCATTCAG
GTGATGGCCA CGGAGCTGGC ACGGAGCCTG AACTGCTCAG GAGAAGAGGC TTCTGAAAGT
TTGATGAACC TCAGCCCTTT CGATATGAAA AATCTCCTGC ACCATATTCT AAGTGGGAAA
GAGTTTGGCG TTGAAAGAAG TGTGCGCCCT ATCCACTCCT CCACATCCAG CCCTACCATC
TCCATCCACG AGGTGGGCCA TACCGGAGTC ACCAAAACTG AGAGGAGTGG CATTAACAGA
CTGAGGAGTG AAATGAAACA GATGACTAGG CGGTTTAGTG CTGATGAACA GTTCTTTTCT
GTGGGCCAGG CCGCGTCCAG CAGTGCGCAT TCCTCCAAGT CTGCGGTAAC AGTGCCTCGG
GATTATTGTA GGTCCAGCAC CCCATCCTCG CCCACTGGCA CGTCATCCTC AGACTCGGGA
GGACATCACA TCGGCTGGGG TGAGCGGCAG GGCCAGTGGC TGCGCAGGAG AAGGCTGGAT
GGGGCCATCA ACAGGGTCCC CGTGGGATTC TACCAGAGGG TGTGGAAGAT CCTCCAGAAG
TGCCACGGTC TCTCCATCGA TGGTTATGTC CTCCCATCCT CGACGACCCG AGAGATGACC
CCGCATGAGA TCAAGTTTGC TGTCCATGTC GAATCGGTGC TGAACCGCGT GCCGCAGCCC
GAGTACCGGC AGCTGCTGGT GGAAGCCATC ATGGTGCTGA CGCTGCTCTC GGACACGGAG
ATGACCAGCA TCGGGGGCAT CATCCACGTG GACCAGATCG TGCAGATGGC CAGTCAGCTG
TTCTTGCAGG ACCAGGTGTC AATTGGTGCC ATGGACACCC TGGAGAAAGA CCAAGCCACA
GGAATCTGCC ACTTCTTTTA TGACAGCGCT CCGAGTGGGG CTTATGGGAC GATGACCTAC
CTAACAAGAG CAGTGGCTTC TTATTTGCAG GAATTGTTGC CCAATTCGGG CTGCCAGATG
CAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006724561.1
CDS401..3586
Misc Feature(1)401..>1171(+)
Misc Feature(2)2258..2611(+)
Translation

Target ORF information:

RefSeq Version XM_006724498
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phosphorylase kinase, alpha 2 (liver) (PHKA2), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006724498

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGTGGGAGC GTGGAGATAA GACTAATCAG GGCATCCCGG AATTGAATGC AAGCTCCGTA 
GGAATGGCCA AGGCAGCTCT TGAGGCAATT GATGAACTGG ACCTTTTTGG AGCCCATGGA
GGACGCAAGT CAGTGATTCA TGTTCTGCCA GATGAGGTCG AGCACTGCCA GTCTATTCTG
TTCTCCATGC TGCCAAGAGC GTCGACATCT AAAGAAATTG ATGCTGGACT TCTTTCCATT
ATTTCCTTCC CGGCCTTTGC AGTGGAAGAT GTAAACCTTG TAAATGTGAC CAAAAATGAA
ATTATTTCTA AGCTCCAGGG GCGTTATGGA TGCTGTCGCT TCCTTCGAGA TGGTTATAAA
ACTCCAAGAG AGGACCCTAA TCGACTGCAT TATGACCCTG CTGAACTCAA GCTCTTCGAA
AACATTGAAT GTGAGTGGCC TGTGTTTTGG ACATATTTTA TAATAGATGG AGTCTTCAGT
GGTGATGCTG TTCAGGTCCA AGAATACCGA GAGGCCCTGG AGGGAATACT CATCAGAGGC
AAGAATGGGA TCCGCCTGGT GCCTGAACTC TACGCTGTCC CGCCTAACAA GGTAGATGAA
GAGTACAAGA ATCCTCACAC AGTAGACCGA GTTCCTATGG GGAAGGTGCC TCATCTGTGG
GGCCAATCCT TGTACATCCT CAGCTCGCTG TTGGCAGAGG GATTCCTTGC CGCTGGTGAA
ATCGATCCCT TAAATAGAAG ATTTTCCACT TCAGTCAAAC CTGATGTTGT AGTACAAGTT
ACTGTTTTGG CAGAAAACAA TCACATTAAG GACTTATTGA GGAAACACGG GGTGAACGTC
CAGAGTATCG CGGACATTCA TCCAATTCAA GTCCAGCCGG GCCGGATTCT TAGTCACATA
TATGCCAAGC TTGGACGGAA TAAGAATATG AATTTGAGTG GGCGACCGTA TCGACATATT
GGTGTCCTTG GAACCTCTAA ACTATATGTG ATTAGGAACC AAATCTTTAC TTTTACACCC
CAGTTCACCG ACCAGCATCA CTTCTACCTG GCCCTCGACA ATGAGATGAT CGTGGAGATG
CTAAGGATCG AGCTGGCCTA CCTGTGCACC TGCTGGAGGA TGACGGGCAG ACCCACACTC
ACCTTCCCCA TCAGTCGCAC CATGCTCACA AATGATGGCT CAGACATTCA TTCTGCTGTG
CTCTCCACAA TTAGAAAACT AGAGGATGGA TATTTTGGAG GAGCCAGAGT AAAATTAGGG
AACCTTTCGG AATTTCTCAC CACATCGTTC TACACATATC TGACTTTTCT GGATCCAGAC
TGTGATGAGA AGTTGTTTGA CAATGCCAGC GAAGGGACTT TCAGTCCTGA TAGTGATTCA
GATTTGGTAG GATATCTGGA AGACACCTGT AATCAAGAAA GCCAAGACGA ACTTGACCAT
TATATCAACC ACCTTCTGCA AAGCACATCG TTGAGGTCCT ATCTGCCTCC TCTTTGTAAG
AACACAGAAG ACCGCCATGT CTTCAGTGCT ATCCACTCCA CGCGGGACAT ACTTTCTGTG
ATGGCAAAAG CAAAGGGTTT GGAAGTTCCA TTTGTTCCCA TGACTTTGCC GACTAAAGTT
CTAAGTGCCC ACCGTAAATC ACTGAATCTT GTTGATTCTC CTCAGCCACT CCTAGAAAAG
GTTCCTGAAA GTGACTTTCA GTGGCCCAGA GATGACCATG GTGACGTGGA CTGTGAGAAG
CTGGTTGAGC AGCTAAAAGA TTGTTCGAAC CTACAGGACC AAGCAGACAT TCTGTACATT
CTTTATGTCA TAAAGGGTCC CAGCTGGGAC ACAAATCTCT CTGGACAGCA CGGGGTCACC
GTTCAAAACC TTCTTGGTGA GCTCTATGGG AAAGCCGGCT TGAACCAGGA GTGGGGTCTG
ATTCGCTACA TCTCAGGCCT TCTCAGGAAG AAAGTGGAGG TCCTGGCTGA GGCCTGCACA
GACCTGCTTT CGCACCAGAA GCAGCTCACC GTGGGCCTGC CGCCCGAGCC CCGGGAGAAG
ATCATCTCTG CGCCCCTTCC CCCAGAGGAG CTCACAAAAC TCATCTACGA GGCCAGTGGG
CAGGACATCA GCATTGCCGT CCTCACGCAG GAGATTGTGG TTTACCTGGC CATGTATGTC
AGGGCGCAGC CCAGCCTCTT TGTGGAGATG CTGAGACTCC GGATTGGACT GATCATTCAG
GTGATGGCCA CGGAGCTGGC ACGGAGCCTG AACTGCTCAG GAGAAGAGGC TTCTGAAAGT
TTGATGAACC TCAGCCCTTT CGATATGAAA AATCTCCTGC ACCATATTCT AAGTGGGAAA
GAGTTTGGCG TTGAAAGAAG TGTGCGCCCT ATCCACTCCT CCACATCCAG CCCTACCATC
TCCATCCACG AGGTGGGCCA TACCGGAGTC ACCAAAACTG AGAGGAGTGG CATTAACAGA
CTGAGGAGTG AAATGAAACA GATGACTAGG CGGTTTAGTG CTGATGAACA GTTCTTTTCT
GTGGGCCAGG CCGCGTCCAG CAGTGCGCAT TCCTCCAAGT CTGCGGTAAC AGTGCCTCGG
GATTATTGTA GGTCCAGCAC CCCATCCTCG CCCACTGGCA CGTCATCCTC AGACTCGGGA
GGACATCACA TCGGCTGGGG TGAGCGGCAG GGCCAGTGGC TGCGCAGGAG AAGGCTGGAT
GGGGCCATCA ACAGGGTCCC CGTGGGATTC TACCAGAGGG TGTGGAAGAT CCTCCAGAAG
TGCCACGGTC TCTCCATCGA TGGTTATGTC CTCCCATCCT CGACGACCCG AGAGATGACC
CCGCATGAGA TCAAGTTTGC TGTCCATGTC GAATCGGTGC TGAACCGCGT GCCGCAGCCC
GAGTACCGGC AGCTGCTGGT GGAAGCCATC ATGGTGCTGA CGCTGCTCTC GGACACGGAG
ATGACCAGCA TCGGGGGCAT CATCCACGTG GACCAGATCG TGCAGATGGC CAGTCAGCTG
TTCTTGCAGG ACCAGGTGTC AATTGGTGCC ATGGACACCC TGGAGAAAGA CCAAGCCACA
GGAATCTGCC ACTTCTTTTA TGACAGCGCT CCGAGTGGGG CTTATGGGAC GATGACCTAC
CTAACAAGAG CAGTGGCTTC TTATTTGCAG GAATTGTTGC CCAATTCGGG CTGCCAGATG
CAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59672
Clone ID Related Accession (Same CDS sequence) XM_011545538
Accession Version XM_011545538.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2715bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167197.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGATGATG GGGCTGTTTT CCAGGTCCAA GAATACCGAG AGGCCCTGGA GGGAATACTC 
ATCAGAGGCA AGAATGGGAT CCGCCTGGTG CCTGAACTCT ACGCTGTCCC GCCTAACAAG
GTAGATGAAG AGTACAAGAA TCCTCACACA GTAGACCGAG TTCCTATGGG GAAGGTGCCT
CATCTGTGGG GCCAATCCTT GTACATCCTC AGCTCGCTGT TGGCAGAGGG ATTCCTTGCC
GCTGGTGAAA TCGATCCCTT AAATAGAAGA TTTTCCACTT CAGTCAAACC TGATGTTGTA
GTACAAGTTA CTGTTTTGGC AGAAAACAAT CACATTAAGG ACTTATTGAG GAAACACGGG
GTGAACGTCC AGAGTATCGC GGACATTCAT CCAATTCAAG TCCAGCCGGG CCGGATTCTT
AGTCACATAT ATGCCAAGCT TGGACGGAAT AAGAATATGA ATTTGAGTGG GCGACCGTAT
CGACATATTG GTGTCCTTGG AACCTCTAAA CTATATGTGA TTAGGAACCA AATCTTTACT
TTTACACCCC AGTTCACCGA CCAGCATCAC TTCTACCTGG CCCTCGACAA TGAGATGATC
GTGGAGATGC TAAGGATCGA GCTGGCCTAC CTGTGCACCT GCTGGAGGAT GACGGGCAGA
CCCACACTCA CCTTCCCCAT CAGTCGCACC ATGCTCACAA ATGATGGCTC AGACATTCAT
TCTGCTGTGC TCTCCACAAT TAGAAAACTA GAGGATGGAT ATTTTGGAGG AGCCAGAGTA
AAATTAGGGA ACCTTTCGGA ATTTCTCACC ACATCGTTCT ACACATATCT GACTTTTCTG
GATCCAGACT GTGATGAGAA GTTGTTTGAC AATGCCAGCG AAGGGACTTT CAGTCCTGAT
AGTGATTCAG ATTTGGTAGG ATATCTGGAA GACACCTGTA ATCAAGAAAG CCAAGACGAA
CTTGACCATT ATATCAACCA CCTTCTGCAA AGCACATCGT TGAGGTCCTA TCTGCCTCCT
CTTTGTAAGA ACACAGAAGA CCGCCATGTC TTCAGTGCTA TCCACTCCAC GCGGGACATA
CTTTCTGTGA TGGCAAAAGC AAAGGGTTTG GAAGTTCCAT TTGTTCCCAT GACTTTGCCG
ACTAAAGTTC TAAGTGCCCA CCGTAAATCA CTGAATCTTG TTGATTCTCC TCAGCCACTC
CTAGAAAAGG TTCCTGAAAG TGACTTTCAG TGGCCCAGAG ATGACCATGG TGACGTGGAC
TGTGAGAAGC TGGTTGAGCA GCTAAAAGAT TGTTCGAACC TACAGGACCA AGCAGACATT
CTGTACATTC TTTATGTCAT AAAGGGTCCC AGCTGGGACA CAAATCTCTC TGGACAGCAC
GGGGTCACCG TTCAAAACCT TCTTGGTGAG CTCTATGGGA AAGCCGGCTT GAACCAGGAG
TGGGGTCTGA TTCGCTACAT CTCAGGCCTT CTCAGGAAGA AAGTGGAGGT CCTGGCTGAG
GCCTGCACAG ACCTGCTTTC GCACCAGAAG CAGCTCACCG TGGGCCTGCC GCCCGAGCCC
CGGGAGAAGA TCATCTCTGC GCCCCTTCCC CCAGAGGAGC TCACAAAACT CATCTACGAG
GCCAGTGGGC AGGACATCAG CATTGCCGTC CTCACGCAGG AGATTGTGGT TTACCTGGCC
ATGTATGTCA GGGCGCAGCC CAGCCTCTTT GTGGAGATGC TGAGACTCCG GATTGGACTG
ATCATTCAGG TGATGGCCAC GGAGCTGGCA CGGAGCCTGA ACTGCTCAGG AGAAGAGGCT
TCTGAAAGTT TGATGAACCT CAGCCCTTTC GATATGAAAA ATCTCCTGCA CCATATTCTA
AGTGGGAAAG AGTTTGGCGT TGAAAGAAGT GTGCGCCCTA TCCACTCCTC CACATCCAGC
CCTACCATCT CCATCCACGA GGTGGGCCAT ACCGGAGTCA CCAAAACTGA GAGGAGTGGC
ATTAACAGAC TGAGGAGTGA AATGAAACAG ATGACTAGGC GGTTTAGTGC TGATGAACAG
TTCTTTTCTG TGGGCCAGGC CGCGTCCAGC AGTGCGCATT CCTCCAAGTC TGCGGTAACA
GTGCCTCGGG ATTATTGTAG GTCCAGCACC CCATCCTCGC CCACTGGCAC GTCATCCTCA
GACTCGGGAG GACATCACAT CGGCTGGGGT GAGCGGCAGG GCCAGTGGCT GCGCAGGAGA
AGGCTGGATG GGGCCATCAA CAGGGTCCCC GTGGGATTCT ACCAGAGGGT GTGGAAGATC
CTCCAGAAGT GCCACGGTCT CTCCATCGAT GGTTATGTCC TCCCATCCTC GACGACCCGA
GAGATGACCC CGCATGAGAT CAAGTTTGCT GTCCATGTCG AATCGGTGCT GAACCGCGTG
CCGCAGCCCG AGTACCGGCA GCTGCTGGTG GAAGCCATCA TGGTGCTGAC GCTGCTCTCG
GACACGGAGA TGACCAGCAT CGGGGGCATC ATCCACGTGG ACCAGATCGT GCAGATGGCC
AGTCAGCTGT TCTTGCAGGA CCAGGTGTCA ATTGGTGCCA TGGACACCCT GGAGAAAGAC
CAAGCCACAG GAATCTGCCA CTTCTTTTAT GACAGCGCTC CGAGTGGGGC TTATGGGACG
ATGACCTACC TAACAAGAGC AGTGGCTTCT TATTTGCAGG AATTGTTGCC CAATTCGGGC
TGCCAGATGC AATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011543840.1
CDS118..2832
Misc Feature(1)148..>417(+)
Misc Feature(2)1504..1857(+)
Translation

Target ORF information:

RefSeq Version XM_011545538
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phosphorylase kinase, alpha 2 (liver) (PHKA2), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011545538

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGATGATG GGGCTGTTTT CCAGGTCCAA GAATACCGAG AGGCCCTGGA GGGAATACTC 
ATCAGAGGCA AGAATGGGAT CCGCCTGGTG CCTGAACTCT ACGCTGTCCC GCCTAACAAG
GTAGATGAAG AGTACAAGAA TCCTCACACA GTAGACCGAG TTCCTATGGG GAAGGTGCCT
CATCTGTGGG GCCAATCCTT GTACATCCTC AGCTCGCTGT TGGCAGAGGG ATTCCTTGCC
GCTGGTGAAA TCGATCCCTT AAATAGAAGA TTTTCCACTT CAGTCAAACC TGATGTTGTA
GTACAAGTTA CTGTTTTGGC AGAAAACAAT CACATTAAGG ACTTATTGAG GAAACACGGG
GTGAACGTCC AGAGTATCGC GGACATTCAT CCAATTCAAG TCCAGCCGGG CCGGATTCTT
AGTCACATAT ATGCCAAGCT TGGACGGAAT AAGAATATGA ATTTGAGTGG GCGACCGTAT
CGACATATTG GTGTCCTTGG AACCTCTAAA CTATATGTGA TTAGGAACCA AATCTTTACT
TTTACACCCC AGTTCACCGA CCAGCATCAC TTCTACCTGG CCCTCGACAA TGAGATGATC
GTGGAGATGC TAAGGATCGA GCTGGCCTAC CTGTGCACCT GCTGGAGGAT GACGGGCAGA
CCCACACTCA CCTTCCCCAT CAGTCGCACC ATGCTCACAA ATGATGGCTC AGACATTCAT
TCTGCTGTGC TCTCCACAAT TAGAAAACTA GAGGATGGAT ATTTTGGAGG AGCCAGAGTA
AAATTAGGGA ACCTTTCGGA ATTTCTCACC ACATCGTTCT ACACATATCT GACTTTTCTG
GATCCAGACT GTGATGAGAA GTTGTTTGAC AATGCCAGCG AAGGGACTTT CAGTCCTGAT
AGTGATTCAG ATTTGGTAGG ATATCTGGAA GACACCTGTA ATCAAGAAAG CCAAGACGAA
CTTGACCATT ATATCAACCA CCTTCTGCAA AGCACATCGT TGAGGTCCTA TCTGCCTCCT
CTTTGTAAGA ACACAGAAGA CCGCCATGTC TTCAGTGCTA TCCACTCCAC GCGGGACATA
CTTTCTGTGA TGGCAAAAGC AAAGGGTTTG GAAGTTCCAT TTGTTCCCAT GACTTTGCCG
ACTAAAGTTC TAAGTGCCCA CCGTAAATCA CTGAATCTTG TTGATTCTCC TCAGCCACTC
CTAGAAAAGG TTCCTGAAAG TGACTTTCAG TGGCCCAGAG ATGACCATGG TGACGTGGAC
TGTGAGAAGC TGGTTGAGCA GCTAAAAGAT TGTTCGAACC TACAGGACCA AGCAGACATT
CTGTACATTC TTTATGTCAT AAAGGGTCCC AGCTGGGACA CAAATCTCTC TGGACAGCAC
GGGGTCACCG TTCAAAACCT TCTTGGTGAG CTCTATGGGA AAGCCGGCTT GAACCAGGAG
TGGGGTCTGA TTCGCTACAT CTCAGGCCTT CTCAGGAAGA AAGTGGAGGT CCTGGCTGAG
GCCTGCACAG ACCTGCTTTC GCACCAGAAG CAGCTCACCG TGGGCCTGCC GCCCGAGCCC
CGGGAGAAGA TCATCTCTGC GCCCCTTCCC CCAGAGGAGC TCACAAAACT CATCTACGAG
GCCAGTGGGC AGGACATCAG CATTGCCGTC CTCACGCAGG AGATTGTGGT TTACCTGGCC
ATGTATGTCA GGGCGCAGCC CAGCCTCTTT GTGGAGATGC TGAGACTCCG GATTGGACTG
ATCATTCAGG TGATGGCCAC GGAGCTGGCA CGGAGCCTGA ACTGCTCAGG AGAAGAGGCT
TCTGAAAGTT TGATGAACCT CAGCCCTTTC GATATGAAAA ATCTCCTGCA CCATATTCTA
AGTGGGAAAG AGTTTGGCGT TGAAAGAAGT GTGCGCCCTA TCCACTCCTC CACATCCAGC
CCTACCATCT CCATCCACGA GGTGGGCCAT ACCGGAGTCA CCAAAACTGA GAGGAGTGGC
ATTAACAGAC TGAGGAGTGA AATGAAACAG ATGACTAGGC GGTTTAGTGC TGATGAACAG
TTCTTTTCTG TGGGCCAGGC CGCGTCCAGC AGTGCGCATT CCTCCAAGTC TGCGGTAACA
GTGCCTCGGG ATTATTGTAG GTCCAGCACC CCATCCTCGC CCACTGGCAC GTCATCCTCA
GACTCGGGAG GACATCACAT CGGCTGGGGT GAGCGGCAGG GCCAGTGGCT GCGCAGGAGA
AGGCTGGATG GGGCCATCAA CAGGGTCCCC GTGGGATTCT ACCAGAGGGT GTGGAAGATC
CTCCAGAAGT GCCACGGTCT CTCCATCGAT GGTTATGTCC TCCCATCCTC GACGACCCGA
GAGATGACCC CGCATGAGAT CAAGTTTGCT GTCCATGTCG AATCGGTGCT GAACCGCGTG
CCGCAGCCCG AGTACCGGCA GCTGCTGGTG GAAGCCATCA TGGTGCTGAC GCTGCTCTCG
GACACGGAGA TGACCAGCAT CGGGGGCATC ATCCACGTGG ACCAGATCGT GCAGATGGCC
AGTCAGCTGT TCTTGCAGGA CCAGGTGTCA ATTGGTGCCA TGGACACCCT GGAGAAAGAC
CAAGCCACAG GAATCTGCCA CTTCTTTTAT GACAGCGCTC CGAGTGGGGC TTATGGGACG
ATGACCTACC TAACAAGAGC AGTGGCTTCT TATTTGCAGG AATTGTTGCC CAATTCGGGC
TGCCAGATGC AATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18889
Clone ID Related Accession (Same CDS sequence) NM_000292
Accession Version NM_000292.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3708bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product phosphorylase b kinase regulatory subunit alpha, liver isoform
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB458123.1, BC014036.2, X80497.1, CD722555.1 and W63591.1. This sequence is a reference standard in the RefSeqGene project. On Mar 12, 2008 this sequence version replaced gi:4505780. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X80497.1, BC014036.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGCGGAGCA GGAGCAATTC CGGGGTCCGC TTGGACGGGT ACGCGCGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA TCCCGTCACG GGGCTGCTGT CAGCCAGCCA TGAGCAGAAG
GATGCCTGGG TGCGGGATAA CATCTACAGT ATCCTGGCCG TGTGGGGCCT GGGCATGGCC
TACCGTAAGA ATGCAGACCG CGATGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAAC
GTGGTGAAGC TGATGCGAGG TCTTCTCCAG TGCATGATGA GACAGGTGGC CAAAGTGGAG
AAGTTCAAAC ACACTCAGAG CACCAAGGAC AGCCTGCACG CCAAGTACAA CACCGCCACC
TGTGGCACGG TGGTGGGCGA CGACCAGTGG GGCCACCTCC AGGTGGATGC CACCTCTCTC
TTCCTCCTGT TCCTGGCCCA GATGACCGCC TCAGGCTTAC GTATCATTTT CACTCTCGAT
GAGGTGGCCT TCATACAGAA TCTTGTCTTT TACATAGAAG CTGCATATAA AGTCGCTGAT
TATGGAATGT GGGAGCGTGG AGATAAGACT AATCAGGGCA TCCCGGAATT GAATGCAAGC
TCCGTAGGAA TGGCCAAGGC AGCTCTTGAG GCAATTGATG AACTGGACCT TTTTGGAGCC
CATGGAGGAC GCAAGTCAGT GATTCATGTT CTGCCAGATG AGGTCGAGCA CTGCCAGTCT
ATTCTGTTCT CCATGCTGCC AAGAGCGTCG ACATCTAAAG AAATTGATGC TGGACTTCTT
TCCATTATTT CCTTCCCGGC CTTTGCAGTG GAAGATGTAA ACCTTGTAAA TGTGACCAAA
AATGAAATTA TTTCTAAGCT CCAGGGGCGT TATGGATGCT GTCGCTTCCT TCGAGATGGT
TATAAAACTC CAAGAGAGGA CCCTAATCGA CTGCATTATG ACCCTGCTGA ACTCAAGCTC
TTCGAAAACA TTGAATGTGA GTGGCCTGTG TTTTGGACAT ATTTTATAAT AGATGGAGTC
TTCAGTGGTG ATGCTGTTCA GGTCCAAGAA TACCGAGAGG CCCTGGAGGG AATACTCATC
AGAGGCAAGA ATGGGATCCG CCTGGTGCCT GAACTCTACG CTGTCCCGCC TAACAAGGTA
GATGAAGAGT ACAAGAATCC TCACACAGTA GACCGAGTTC CTATGGGGAA GGTGCCTCAT
CTGTGGGGCC AATCCTTGTA CATCCTCAGC TCGCTGTTGG CAGAGGGATT CCTTGCCGCT
GGTGAAATCG ATCCCTTAAA TAGAAGATTT TCCACTTCAG TCAAACCTGA TGTTGTAGTA
CAAGTTACTG TTTTGGCAGA AAACAATCAC ATTAAGGACT TATTGAGGAA ACACGGGGTG
AACGTCCAGA GTATCGCGGA CATTCATCCA ATTCAAGTCC AGCCGGGCCG GATTCTTAGT
CACATATATG CCAAGCTTGG ACGGAATAAG AATATGAATT TGAGTGGGCG ACCGTATCGA
CATATTGGTG TCCTTGGAAC CTCTAAACTA TATGTGATTA GGAACCAAAT CTTTACTTTT
ACACCCCAGT TCACCGACCA GCATCACTTC TACCTGGCCC TCGACAATGA GATGATCGTG
GAGATGCTAA GGATCGAGCT GGCCTACCTG TGCACCTGCT GGAGGATGAC GGGCAGACCC
ACACTCACCT TCCCCATCAG TCGCACCATG CTCACAAATG ATGGCTCAGA CATTCATTCT
GCTGTGCTCT CCACAATTAG AAAACTAGAG GATGGATATT TTGGAGGAGC CAGAGTAAAA
TTAGGGAACC TTTCGGAATT TCTCACCACA TCGTTCTACA CATATCTGAC TTTTCTGGAT
CCAGACTGTG ATGAGAAGTT GTTTGACAAT GCCAGCGAAG GGACTTTCAG TCCTGATAGT
GATTCAGATT TGGTAGGATA TCTGGAAGAC ACCTGTAATC AAGAAAGCCA AGACGAACTT
GACCATTATA TCAACCACCT TCTGCAAAGC ACATCGTTGA GGTCCTATCT GCCTCCTCTT
TGTAAGAACA CAGAAGACCG CCATGTCTTC AGTGCTATCC ACTCCACGCG GGACATACTT
TCTGTGATGG CAAAAGCAAA GGGTTTGGAA GTTCCATTTG TTCCCATGAC TTTGCCGACT
AAAGTTCTAA GTGCCCACCG TAAATCACTG AATCTTGTTG ATTCTCCTCA GCCACTCCTA
GAAAAGGTTC CTGAAAGTGA CTTTCAGTGG CCCAGAGATG ACCATGGTGA CGTGGACTGT
GAGAAGCTGG TTGAGCAGCT AAAAGATTGT TCGAACCTAC AGGACCAAGC AGACATTCTG
TACATTCTTT ATGTCATAAA GGGTCCCAGC TGGGACACAA ATCTCTCTGG ACAGCACGGG
GTCACCGTTC AAAACCTTCT TGGTGAGCTC TATGGGAAAG CCGGCTTGAA CCAGGAGTGG
GGTCTGATTC GCTACATCTC AGGCCTTCTC AGGAAGAAAG TGGAGGTCCT GGCTGAGGCC
TGCACAGACC TGCTTTCGCA CCAGAAGCAG CTCACCGTGG GCCTGCCGCC CGAGCCCCGG
GAGAAGATCA TCTCTGCGCC CCTTCCCCCA GAGGAGCTCA CAAAACTCAT CTACGAGGCC
AGTGGGCAGG ACATCAGCAT TGCCGTCCTC ACGCAGGAGA TTGTGGTTTA CCTGGCCATG
TATGTCAGGG CGCAGCCCAG CCTCTTTGTG GAGATGCTGA GACTCCGGAT TGGACTGATC
ATTCAGGTGA TGGCCACGGA GCTGGCACGG AGCCTGAACT GCTCAGGAGA AGAGGCTTCT
GAAAGTTTGA TGAACCTCAG CCCTTTCGAT ATGAAAAATC TCCTGCACCA TATTCTAAGT
GGGAAAGAGT TTGGCGTTGA AAGAAGTGTG CGCCCTATCC ACTCCTCCAC ATCCAGCCCT
ACCATCTCCA TCCACGAGGT GGGCCATACC GGAGTCACCA AAACTGAGAG GAGTGGCATT
AACAGACTGA GGAGTGAAAT GAAACAGATG ACTAGGCGGT TTAGTGCTGA TGAACAGTTC
TTTTCTGTGG GCCAGGCCGC GTCCAGCAGT GCGCATTCCT CCAAGTCTGC GAGGTCCAGC
ACCCCATCCT CGCCCACTGG CACGTCATCC TCAGACTCGG GAGGACATCA CATCGGCTGG
GGTGAGCGGC AGGGCCAGTG GCTGCGCAGG AGAAGGCTGG ATGGGGCCAT CAACAGGGTC
CCCGTGGGAT TCTACCAGAG GGTGTGGAAG ATCCTCCAGA AGTGCCACGG TCTCTCCATC
GATGGTTATG TCCTCCCATC CTCGACGACC CGAGAGATGA CCCCGCATGA GATCAAGTTT
GCTGTCCATG TCGAATCGGT GCTGAACCGC GTGCCGCAGC CCGAGTACCG GCAGCTGCTG
GTGGAAGCCA TCATGGTGCT GACGCTGCTC TCGGACACGG AGATGACCAG CATCGGGGGC
ATCATCCACG TGGACCAGAT CGTGCAGATG GCCAGTCAGC TGTTCTTGCA GGACCAGGTG
TCAATTGGTG CCATGGACAC CCTGGAGAAA GACCAAGCCA CAGGAATCTG CCACTTCTTT
TATGACAGCG CTCCGAGTGG GGCTTATGGG ACGATGACCT ACCTAACAAG AGCAGTGGCT
TCTTATTTGC AGGAATTGTT GCCCAATTCG GGCTGCCAGA TGCAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000283.1
CDS431..4138
Misc Feature(1)335..337(+)
Misc Feature(2)452..>1747(+)
Misc Feature(3)2615..2617(+)
Misc Feature(4)2633..2635(+)
Misc Feature(5)2633..2635(+)
Misc Feature(6)2834..3187(+)
Misc Feature(7)2849..2941(+)
Misc Feature(8)3473..3475(+)
Misc Feature(9)3605..3727(+)
Exon (1)1..508
Gene:PHKA2
Gene Synonym:
Exon (2)509..667
Gene:PHKA2
Gene Synonym:
Exon (3)668..715
Gene:PHKA2
Gene Synonym:
Exon (4)716..884
Gene:PHKA2
Gene Synonym:
Exon (5)885..967
Gene:PHKA2
Gene Synonym:
Exon (6)968..1048
Gene:PHKA2
Gene Synonym:
Exon (7)1049..1147
Gene:PHKA2
Gene Synonym:
Exon (8)1148..1294
Gene:PHKA2
Gene Synonym:
Exon (9)1295..1348
Gene:PHKA2
Gene Synonym:
Exon (10)1349..1471
Gene:PHKA2
Gene Synonym:
Exon (11)1472..1567
Gene:PHKA2
Gene Synonym:
Exon (12)1568..1675
Gene:PHKA2
Gene Synonym:
Exon (13)1676..1754
Gene:PHKA2
Gene Synonym:
Exon (14)1755..1889
Gene:PHKA2
Gene Synonym:
Exon (15)1890..1999
Gene:PHKA2
Gene Synonym:
Exon (16)2000..2144
Gene:PHKA2
Gene Synonym:
Exon (17)2145..2223
Gene:PHKA2
Gene Synonym:
Exon (18)2224..2393
Gene:PHKA2
Gene Synonym:
Exon (19)2394..2567
Gene:PHKA2
Gene Synonym:
Exon (20)2568..2656
Gene:PHKA2
Gene Synonym:
Exon (21)2657..2790
Gene:PHKA2
Gene Synonym:
Exon (22)2791..2947
Gene:PHKA2
Gene Synonym:
Exon (23)2948..3027
Gene:PHKA2
Gene Synonym:
Exon (24)3028..3106
Gene:PHKA2
Gene Synonym:
Exon (25)3107..3236
Gene:PHKA2
Gene Synonym:
Exon (26)3237..3338
Gene:PHKA2
Gene Synonym:
Exon (27)3339..3457
Gene:PHKA2
Gene Synonym:
Exon (28)3458..3487
Gene:PHKA2
Gene Synonym:
Exon (29)3488..3541
Gene:PHKA2
Gene Synonym:
Exon (30)3542..3712
Gene:PHKA2
Gene Synonym:
Exon (31)3713..3766
Gene:PHKA2
Gene Synonym:
Exon (32)3767..3967
Gene:PHKA2
Gene Synonym:
Exon (33)3968..5325
Gene:PHKA2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000292
Organism Homo sapiens (human)
Definition Homo sapiens phosphorylase kinase, alpha 2 (liver) (PHKA2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000292

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGCGGAGCA GGAGCAATTC CGGGGTCCGC TTGGACGGGT ACGCGCGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA TCCCGTCACG GGGCTGCTGT CAGCCAGCCA TGAGCAGAAG
GATGCCTGGG TGCGGGATAA CATCTACAGT ATCCTGGCCG TGTGGGGCCT GGGCATGGCC
TACCGTAAGA ATGCAGACCG CGATGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAAC
GTGGTGAAGC TGATGCGAGG TCTTCTCCAG TGCATGATGA GACAGGTGGC CAAAGTGGAG
AAGTTCAAAC ACACTCAGAG CACCAAGGAC AGCCTGCACG CCAAGTACAA CACCGCCACC
TGTGGCACGG TGGTGGGCGA CGACCAGTGG GGCCACCTCC AGGTGGATGC CACCTCTCTC
TTCCTCCTGT TCCTGGCCCA GATGACCGCC TCAGGCTTAC GTATCATTTT CACTCTCGAT
GAGGTGGCCT TCATACAGAA TCTTGTCTTT TACATAGAAG CTGCATATAA AGTCGCTGAT
TATGGAATGT GGGAGCGTGG AGATAAGACT AATCAGGGCA TCCCGGAATT GAATGCAAGC
TCCGTAGGAA TGGCCAAGGC AGCTCTTGAG GCAATTGATG AACTGGACCT TTTTGGAGCC
CATGGAGGAC GCAAGTCAGT GATTCATGTT CTGCCAGATG AGGTCGAGCA CTGCCAGTCT
ATTCTGTTCT CCATGCTGCC AAGAGCGTCG ACATCTAAAG AAATTGATGC TGGACTTCTT
TCCATTATTT CCTTCCCGGC CTTTGCAGTG GAAGATGTAA ACCTTGTAAA TGTGACCAAA
AATGAAATTA TTTCTAAGCT CCAGGGGCGT TATGGATGCT GTCGCTTCCT TCGAGATGGT
TATAAAACTC CAAGAGAGGA CCCTAATCGA CTGCATTATG ACCCTGCTGA ACTCAAGCTC
TTCGAAAACA TTGAATGTGA GTGGCCTGTG TTTTGGACAT ATTTTATAAT AGATGGAGTC
TTCAGTGGTG ATGCTGTTCA GGTCCAAGAA TACCGAGAGG CCCTGGAGGG AATACTCATC
AGAGGCAAGA ATGGGATCCG CCTGGTGCCT GAACTCTACG CTGTCCCGCC TAACAAGGTA
GATGAAGAGT ACAAGAATCC TCACACAGTA GACCGAGTTC CTATGGGGAA GGTGCCTCAT
CTGTGGGGCC AATCCTTGTA CATCCTCAGC TCGCTGTTGG CAGAGGGATT CCTTGCCGCT
GGTGAAATCG ATCCCTTAAA TAGAAGATTT TCCACTTCAG TCAAACCTGA TGTTGTAGTA
CAAGTTACTG TTTTGGCAGA AAACAATCAC ATTAAGGACT TATTGAGGAA ACACGGGGTG
AACGTCCAGA GTATCGCGGA CATTCATCCA ATTCAAGTCC AGCCGGGCCG GATTCTTAGT
CACATATATG CCAAGCTTGG ACGGAATAAG AATATGAATT TGAGTGGGCG ACCGTATCGA
CATATTGGTG TCCTTGGAAC CTCTAAACTA TATGTGATTA GGAACCAAAT CTTTACTTTT
ACACCCCAGT TCACCGACCA GCATCACTTC TACCTGGCCC TCGACAATGA GATGATCGTG
GAGATGCTAA GGATCGAGCT GGCCTACCTG TGCACCTGCT GGAGGATGAC GGGCAGACCC
ACACTCACCT TCCCCATCAG TCGCACCATG CTCACAAATG ATGGCTCAGA CATTCATTCT
GCTGTGCTCT CCACAATTAG AAAACTAGAG GATGGATATT TTGGAGGAGC CAGAGTAAAA
TTAGGGAACC TTTCGGAATT TCTCACCACA TCGTTCTACA CATATCTGAC TTTTCTGGAT
CCAGACTGTG ATGAGAAGTT GTTTGACAAT GCCAGCGAAG GGACTTTCAG TCCTGATAGT
GATTCAGATT TGGTAGGATA TCTGGAAGAC ACCTGTAATC AAGAAAGCCA AGACGAACTT
GACCATTATA TCAACCACCT TCTGCAAAGC ACATCGTTGA GGTCCTATCT GCCTCCTCTT
TGTAAGAACA CAGAAGACCG CCATGTCTTC AGTGCTATCC ACTCCACGCG GGACATACTT
TCTGTGATGG CAAAAGCAAA GGGTTTGGAA GTTCCATTTG TTCCCATGAC TTTGCCGACT
AAAGTTCTAA GTGCCCACCG TAAATCACTG AATCTTGTTG ATTCTCCTCA GCCACTCCTA
GAAAAGGTTC CTGAAAGTGA CTTTCAGTGG CCCAGAGATG ACCATGGTGA CGTGGACTGT
GAGAAGCTGG TTGAGCAGCT AAAAGATTGT TCGAACCTAC AGGACCAAGC AGACATTCTG
TACATTCTTT ATGTCATAAA GGGTCCCAGC TGGGACACAA ATCTCTCTGG ACAGCACGGG
GTCACCGTTC AAAACCTTCT TGGTGAGCTC TATGGGAAAG CCGGCTTGAA CCAGGAGTGG
GGTCTGATTC GCTACATCTC AGGCCTTCTC AGGAAGAAAG TGGAGGTCCT GGCTGAGGCC
TGCACAGACC TGCTTTCGCA CCAGAAGCAG CTCACCGTGG GCCTGCCGCC CGAGCCCCGG
GAGAAGATCA TCTCTGCGCC CCTTCCCCCA GAGGAGCTCA CAAAACTCAT CTACGAGGCC
AGTGGGCAGG ACATCAGCAT TGCCGTCCTC ACGCAGGAGA TTGTGGTTTA CCTGGCCATG
TATGTCAGGG CGCAGCCCAG CCTCTTTGTG GAGATGCTGA GACTCCGGAT TGGACTGATC
ATTCAGGTGA TGGCCACGGA GCTGGCACGG AGCCTGAACT GCTCAGGAGA AGAGGCTTCT
GAAAGTTTGA TGAACCTCAG CCCTTTCGAT ATGAAAAATC TCCTGCACCA TATTCTAAGT
GGGAAAGAGT TTGGCGTTGA AAGAAGTGTG CGCCCTATCC ACTCCTCCAC ATCCAGCCCT
ACCATCTCCA TCCACGAGGT GGGCCATACC GGAGTCACCA AAACTGAGAG GAGTGGCATT
AACAGACTGA GGAGTGAAAT GAAACAGATG ACTAGGCGGT TTAGTGCTGA TGAACAGTTC
TTTTCTGTGG GCCAGGCCGC GTCCAGCAGT GCGCATTCCT CCAAGTCTGC GAGGTCCAGC
ACCCCATCCT CGCCCACTGG CACGTCATCC TCAGACTCGG GAGGACATCA CATCGGCTGG
GGTGAGCGGC AGGGCCAGTG GCTGCGCAGG AGAAGGCTGG ATGGGGCCAT CAACAGGGTC
CCCGTGGGAT TCTACCAGAG GGTGTGGAAG ATCCTCCAGA AGTGCCACGG TCTCTCCATC
GATGGTTATG TCCTCCCATC CTCGACGACC CGAGAGATGA CCCCGCATGA GATCAAGTTT
GCTGTCCATG TCGAATCGGT GCTGAACCGC GTGCCGCAGC CCGAGTACCG GCAGCTGCTG
GTGGAAGCCA TCATGGTGCT GACGCTGCTC TCGGACACGG AGATGACCAG CATCGGGGGC
ATCATCCACG TGGACCAGAT CGTGCAGATG GCCAGTCAGC TGTTCTTGCA GGACCAGGTG
TCAATTGGTG CCATGGACAC CCTGGAGAAA GACCAAGCCA CAGGAATCTG CCACTTCTTT
TATGACAGCG CTCCGAGTGG GGCTTATGGG ACGATGACCT ACCTAACAAG AGCAGTGGCT
TCTTATTTGC AGGAATTGTT GCCCAATTCG GGCTGCCAGA TGCAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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