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PHKB cDNA ORF clone, Homo sapiens (human)

Gene Symbol PHKB
Entrez Gene ID 5257
Full Name phosphorylase kinase, beta
General protein information
Preferred Names
phosphorylase b kinase regulatory subunit beta
Names
phosphorylase b kinase regulatory subunit beta
phosphorylase kinase beta-subunit
phosphorylase kinase subunit beta
NP_000284.1
NP_001027005.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

16

16q12-q13

Summary Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The beta subunit is the same in both the muscle and hepatic isoforms, encoded by this gene, which is a member of the phosphorylase b kinase regulatory subunit family. The gamma subunit also includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9B, also known as phosphorylase kinase deficiency of liver and muscle. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. Two pseudogenes have been found on chromosomes 14 and 20, respectively.[provided by RefSeq, Feb 2010]. lac of sum
Disorder MIM:

172490

Disorder Html: Phosphorylase kinase deficiency of liver and muscle, autosomal

mRNA and Protein(s)

mRNA Protein Name
XM_005255983 XP_005256040 phosphorylase b kinase regulatory subunit beta isoform X2
XM_005255984 XP_005256041 phosphorylase b kinase regulatory subunit beta isoform X1
XM_011523106 XP_011521408 phosphorylase b kinase regulatory subunit beta isoform X3
XM_011523107 XP_011521409 phosphorylase b kinase regulatory subunit beta isoform X4
NM_000293 NP_000284 phosphorylase b kinase regulatory subunit beta isoform a
NM_001031835 NP_001027005 phosphorylase b kinase regulatory subunit beta isoform b



Homo sapiens (human) PHKB NP_000284.1
Pan troglodytes (chimpanzee) PHKB XP_001162360.1
Macaca mulatta (Rhesus monkey) PHKB XP_001113858.1
Canis lupus familiaris (dog) PHKB XP_005617652.1
Bos taurus (cattle) PHKB XP_002694819.1
Mus musculus (house mouse) Phkb NP_955517.1
Rattus norvegicus (Norway rat) Phkb NP_001014174.1
Gallus gallus (chicken) PHKB NP_001007832.1
Danio rerio (zebrafish) phkb XP_698197.5
Drosophila melanogaster (fruit fly) CG8475 NP_001260230.1
Caenorhabditis elegans Y67D8A.2 NP_741355.3
Xenopus (Silurana) tropicalis (western clawed frog) phkb XP_004914116.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PHKB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PHKB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu49387 XM_005255983 PREDICTED: Homo sapiens phosphorylase kinase, beta (PHKB), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu16564 XM_005255984 PREDICTED: Homo sapiens phosphorylase kinase, beta (PHKB), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $384.30
$549.00
OHu59673 XM_011523106 PREDICTED: Homo sapiens phosphorylase kinase, beta (PHKB), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu59674 XM_011523107 PREDICTED: Homo sapiens phosphorylase kinase, beta (PHKB), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu18700 NM_000293 Homo sapiens phosphorylase kinase, beta (PHKB), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $776.30
$1109.00
OHu16564 NM_001031835 Homo sapiens phosphorylase kinase, beta (PHKB), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $384.30
$549.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu49387
Clone ID Related Accession (Same CDS sequence) XM_005255983
Accession Version XM_005255983.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3282bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product phosphorylase b kinase regulatory subunit beta isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010498.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578828992. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGGCGGGGG CGGCGGGACT CACGGCAGAA GTGAGCTGGA AGGTCTTGGA GCGAAGAGCT 
CGGACCAAGC GCTCAGGCTC AGTTTATGAA CCTCTTAAAA GCATTAATCT TCCAAGACCT
GATAATGAAA CTCTCTGGGA TAAGTTGGAC CATTATTACA GAATTGTCAA GTCAACATTG
CTGCTGTATC AAAGTCCAAC TACCGGTCTC TTTCCCACTA AAACATGCGG TGGTGACCAG
AAGGCCAAGA TCCAGGACAG CCTATACTGC GCTGCTGGGG CCTGGGCTTT GGCTCTTGCA
TACAGGCGAA TTGATGATGA CAAGGGAAGG ACCCATGAGC TGGAGCACTC AGCTATAAAA
TGCATGAGAG GAATTCTCTA CTGCTATATG CGTCAGGCCG ATAAGGTCCA GCAGTTTAAG
CAGGATCCAC GCCCAACAAC ATGTCTTCAC TCTGTTTTCA ATGTGCATAC AGGAGATGAG
TTGCTTTCCT ATGAGGAATA TGGTCATCTT CAGATAAATG CAGTGTCACT TTATCTCCTT
TACCTTGTGG AAATGATTTC CTCAGGACTC CAGATTATCT ACAACACTGA TGAGGTCTCT
TTTATTCAAA ACCTTGTATT TTGTGTGGAA AGAGTTTACC GTGTGCCTGA CTTTGGTGTC
TGGGAAAGAG GAAGCAAATA TAATAATGGC AGCACAGAGC TACATTCGAG CTCGGTTGGT
TTAGCAAAAG CAGCTCTAGA AGCAATTAAT GGATTCAACC TTTTTGGCAA CCAGGGCTGT
TCGTGGTCAG TTATATTTGT GGATCTCGAT GCTCACAATC GCAACAGGCA AACTTTGTGC
TCGCTGTTAC CCAGAGAATC AAGATCACAT AATACAGATG CTGCCCTGCT CCCCTGCATC
AGTTATCCTG CATTTGCCCT GGATGATGAA GTTCTTTTTA GCCAGACACT TGATAAAGTG
GTTAGAAAAT TAAAAGGAAA ATATGGATTT AAACGTTTCT TGAGAGATGG GTATAGAACA
TCATTGGAAG ATCCCAACAG ATGCTACTAC AAGCCAGCTG AAATTAAGCT ATTTGATGGC
ATTGAATGTG AATTTCCCAT ATTTTTCCTT TATATGATGA TTGATGGAGT TTTTAGAGGC
AATCCTAAGC AAGTACAGGA ATATCAGGAT CTTTTGACTC CAGTACTTCA TCATACCACA
GAAGGATATC CTGTTGTACC AAAGTACTAT TATGTGCCAG CTGACTTTGT AGAATATGAA
AAAAATAACC CTGGTAGTCA AAAACGATTT CCTAGCAACT GTGGCCGTGA TGGAAAACTG
TTTCTTTGGG GACAAGCACT TTATATCATC GCAAAACTCC TGGCTGATGA ACTTATTAGT
CCTAAAGACA TTGATCCTGT CCAGCGCTAT GTCCCACTAA AGGATCAACG TAACGTGAGC
ATGAGGTTTT CCAATCAGGG CCCACTGGAA AATGACTTGG TAGTTCATGT GGCACTTATA
GCAGAAAGCC AAAGACTTCA AGTTTTTCTG AACACATATG GTATTCAAAC TCAAACTCCT
CAACAAGTAG AACCCATTCA GATATGGCCT CAGCAGGAGC TTGTGAAAGC TTATTTGCAG
CTGGGTATCA ATGAAAAGTT AGGACTCTCT GGAAGGCCAG ACAGGCCCAT TGGCTGCCTC
GGGACATCAA AGATTTATCG CATTCTAGGA AAGACTGTGG TTTGTTACCC GATTATTTTC
GACCTAAGTG ATTTCTACAT GTCTCAGGAT GTTTTCCTGC TGATAGATGA CATAAAGAAT
GCGCTGCAGT TCATTAAACA ATATTGGAAA ATGCATGGAC GTCCACTTTT CCTTGTTCTC
ATCCGGGAAG ACAATATAAG AGGTAGCCGG TTCAACCCCA TATTAGATAT GCTGGCAGCC
CTTAAAAAAG GAATAATTGG AGGAGTCAAA GTTCATGTGG ATCGTCTACA GACACTAATA
TCTGGAGCTG TGGTAGAACA ACTTGATTTC CTACGAATCA GTGACACAGA AGAGCTTCCA
GAATTTAAGA GTTTTGAGGA ACTAGAACCT CCCAAACATT CAAAAGTCAA ACGGCAAAGC
AGCACCCCTA GTGCTCCTGA ACTGGGACAG CAGCCGGATG TCAACATTAG TGAATGGAAG
GACAAACCCA CCCACGAAAT TCTTCAAAAA CTGAATGATT GCAGTTGTCT GGCTAGCCAA
GCCATCCTGC TGGGTATACT GCTCAAAAGA GAAGGCCCCA ACTTCATCAC AAAGGAAGGT
ACCGTTTCTG ATCACATTGA GAGAGTCTAT AGAAGAGCTG GCAGCCAAAA ACTTTGGTCG
GTTGTACGCC GTGCAGCAAG TCTTTTAAGT AAAGTAGTGG ACAGCCTGGC CCCATCCATT
ACTAATGTTT TAGTGCAGGG CAAACAGGTA ACTCTGGGTG CCTTTGGGCA TGAAGAAGAA
GTTATCTCTA ATCCTTTGTC TCCAAGAGTG ATTCAAAACA TCATCTATTA TAAGTGTAAC
ACCCATGATG AGAGGGAAGC GGTCATTCAG CAAGAACTGG TCATCCATAT TGGCTGGATC
ATCTCCAATA ACCCTGAGTT ATTCAGTGGC ATGCTGAAAA TACGAATCGG GTGGATCATC
CATGCCATGG AGTATGAACT TCAGATCCGT GGCGGAGACA AGCCAGCCTT GGACTTGTAT
CAGCTGTCAC CTAGTGAAGT TAAACAGCTT CTGCTGGATA TTCTGCAGCC TCAACAGAAT
GGAAGATGTT GGCTGAACAG GCGTCAGATC GATGGGTCTT TGAATAGAAC TCCCACCGGG
TTCTATGACC GAGTGTGGCA GATTCTGGAG CGCACGCCCA ATGGGATCAT TGTTGCTGGG
AAGCATTTGC CTCAGCAACC AACCCTGTCA GATATGACCA TGTATGAGAT GAATTTCTCT
CTCCTTGTTG AAGACACGTT GGGAAATATT GACCAGCCAC AGTACAGACA GATCGTTGTA
GAGTTACTTA TGGTTGTATC CATTGTACTG GAAAGAAACC CCGAGCTAGA ATTTCAAGAC
AAAGTAGATC TAGACAGACT GGTCAAAGAA GCATTTAATG AATTTCAAAA AGATCAGAGT
CGGCTAAAGG AAATTGAAAA ACAAGATGAC ATGACTTCCT TTTACAACAC TCCTCCCCTG
GGAAAAAGAG GAACATGCAG CTATTTGACA AAGGCGGTGA TGAATCTGCT GCTGGAAGGA
GAAGTCAAGC CAAACAATGA TGACCCGTGT CTGATTAGCT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005256040.1
CDS16..3297
Misc Feature(1)160..>1425(+)
Misc Feature(2)2266..2649(+)
Translation

Target ORF information:

RefSeq Version XM_005255983
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phosphorylase kinase, beta (PHKB), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005255983

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGGCGGGGG CGGCGGGACT CACGGCAGAA GTGAGCTGGA AGGTCTTGGA GCGAAGAGCT 
CGGACCAAGC GCTCAGGCTC AGTTTATGAA CCTCTTAAAA GCATTAATCT TCCAAGACCT
GATAATGAAA CTCTCTGGGA TAAGTTGGAC CATTATTACA GAATTGTCAA GTCAACATTG
CTGCTGTATC AAAGTCCAAC TACCGGTCTC TTTCCCACTA AAACATGCGG TGGTGACCAG
AAGGCCAAGA TCCAGGACAG CCTATACTGC GCTGCTGGGG CCTGGGCTTT GGCTCTTGCA
TACAGGCGAA TTGATGATGA CAAGGGAAGG ACCCATGAGC TGGAGCACTC AGCTATAAAA
TGCATGAGAG GAATTCTCTA CTGCTATATG CGTCAGGCCG ATAAGGTCCA GCAGTTTAAG
CAGGATCCAC GCCCAACAAC ATGTCTTCAC TCTGTTTTCA ATGTGCATAC AGGAGATGAG
TTGCTTTCCT ATGAGGAATA TGGTCATCTT CAGATAAATG CAGTGTCACT TTATCTCCTT
TACCTTGTGG AAATGATTTC CTCAGGACTC CAGATTATCT ACAACACTGA TGAGGTCTCT
TTTATTCAAA ACCTTGTATT TTGTGTGGAA AGAGTTTACC GTGTGCCTGA CTTTGGTGTC
TGGGAAAGAG GAAGCAAATA TAATAATGGC AGCACAGAGC TACATTCGAG CTCGGTTGGT
TTAGCAAAAG CAGCTCTAGA AGCAATTAAT GGATTCAACC TTTTTGGCAA CCAGGGCTGT
TCGTGGTCAG TTATATTTGT GGATCTCGAT GCTCACAATC GCAACAGGCA AACTTTGTGC
TCGCTGTTAC CCAGAGAATC AAGATCACAT AATACAGATG CTGCCCTGCT CCCCTGCATC
AGTTATCCTG CATTTGCCCT GGATGATGAA GTTCTTTTTA GCCAGACACT TGATAAAGTG
GTTAGAAAAT TAAAAGGAAA ATATGGATTT AAACGTTTCT TGAGAGATGG GTATAGAACA
TCATTGGAAG ATCCCAACAG ATGCTACTAC AAGCCAGCTG AAATTAAGCT ATTTGATGGC
ATTGAATGTG AATTTCCCAT ATTTTTCCTT TATATGATGA TTGATGGAGT TTTTAGAGGC
AATCCTAAGC AAGTACAGGA ATATCAGGAT CTTTTGACTC CAGTACTTCA TCATACCACA
GAAGGATATC CTGTTGTACC AAAGTACTAT TATGTGCCAG CTGACTTTGT AGAATATGAA
AAAAATAACC CTGGTAGTCA AAAACGATTT CCTAGCAACT GTGGCCGTGA TGGAAAACTG
TTTCTTTGGG GACAAGCACT TTATATCATC GCAAAACTCC TGGCTGATGA ACTTATTAGT
CCTAAAGACA TTGATCCTGT CCAGCGCTAT GTCCCACTAA AGGATCAACG TAACGTGAGC
ATGAGGTTTT CCAATCAGGG CCCACTGGAA AATGACTTGG TAGTTCATGT GGCACTTATA
GCAGAAAGCC AAAGACTTCA AGTTTTTCTG AACACATATG GTATTCAAAC TCAAACTCCT
CAACAAGTAG AACCCATTCA GATATGGCCT CAGCAGGAGC TTGTGAAAGC TTATTTGCAG
CTGGGTATCA ATGAAAAGTT AGGACTCTCT GGAAGGCCAG ACAGGCCCAT TGGCTGCCTC
GGGACATCAA AGATTTATCG CATTCTAGGA AAGACTGTGG TTTGTTACCC GATTATTTTC
GACCTAAGTG ATTTCTACAT GTCTCAGGAT GTTTTCCTGC TGATAGATGA CATAAAGAAT
GCGCTGCAGT TCATTAAACA ATATTGGAAA ATGCATGGAC GTCCACTTTT CCTTGTTCTC
ATCCGGGAAG ACAATATAAG AGGTAGCCGG TTCAACCCCA TATTAGATAT GCTGGCAGCC
CTTAAAAAAG GAATAATTGG AGGAGTCAAA GTTCATGTGG ATCGTCTACA GACACTAATA
TCTGGAGCTG TGGTAGAACA ACTTGATTTC CTACGAATCA GTGACACAGA AGAGCTTCCA
GAATTTAAGA GTTTTGAGGA ACTAGAACCT CCCAAACATT CAAAAGTCAA ACGGCAAAGC
AGCACCCCTA GTGCTCCTGA ACTGGGACAG CAGCCGGATG TCAACATTAG TGAATGGAAG
GACAAACCCA CCCACGAAAT TCTTCAAAAA CTGAATGATT GCAGTTGTCT GGCTAGCCAA
GCCATCCTGC TGGGTATACT GCTCAAAAGA GAAGGCCCCA ACTTCATCAC AAAGGAAGGT
ACCGTTTCTG ATCACATTGA GAGAGTCTAT AGAAGAGCTG GCAGCCAAAA ACTTTGGTCG
GTTGTACGCC GTGCAGCAAG TCTTTTAAGT AAAGTAGTGG ACAGCCTGGC CCCATCCATT
ACTAATGTTT TAGTGCAGGG CAAACAGGTA ACTCTGGGTG CCTTTGGGCA TGAAGAAGAA
GTTATCTCTA ATCCTTTGTC TCCAAGAGTG ATTCAAAACA TCATCTATTA TAAGTGTAAC
ACCCATGATG AGAGGGAAGC GGTCATTCAG CAAGAACTGG TCATCCATAT TGGCTGGATC
ATCTCCAATA ACCCTGAGTT ATTCAGTGGC ATGCTGAAAA TACGAATCGG GTGGATCATC
CATGCCATGG AGTATGAACT TCAGATCCGT GGCGGAGACA AGCCAGCCTT GGACTTGTAT
CAGCTGTCAC CTAGTGAAGT TAAACAGCTT CTGCTGGATA TTCTGCAGCC TCAACAGAAT
GGAAGATGTT GGCTGAACAG GCGTCAGATC GATGGGTCTT TGAATAGAAC TCCCACCGGG
TTCTATGACC GAGTGTGGCA GATTCTGGAG CGCACGCCCA ATGGGATCAT TGTTGCTGGG
AAGCATTTGC CTCAGCAACC AACCCTGTCA GATATGACCA TGTATGAGAT GAATTTCTCT
CTCCTTGTTG AAGACACGTT GGGAAATATT GACCAGCCAC AGTACAGACA GATCGTTGTA
GAGTTACTTA TGGTTGTATC CATTGTACTG GAAAGAAACC CCGAGCTAGA ATTTCAAGAC
AAAGTAGATC TAGACAGACT GGTCAAAGAA GCATTTAATG AATTTCAAAA AGATCAGAGT
CGGCTAAAGG AAATTGAAAA ACAAGATGAC ATGACTTCCT TTTACAACAC TCCTCCCCTG
GGAAAAAGAG GAACATGCAG CTATTTGACA AAGGCGGTGA TGAATCTGCT GCTGGAAGGA
GAAGTCAAGC CAAACAATGA TGACCCGTGT CTGATTAGCT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16564
Clone ID Related Accession (Same CDS sequence) NM_001031835 , XM_005255984
Accession Version XM_005255984.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product phosphorylase b kinase regulatory subunit beta isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010498.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578828993. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGGCCTGCT CACCTGATGC AGTCGTCTCT CCGTCTTCCG CTTTCTTAAG GTCTGGCTCA 
GTTTATGAAC CTCTTAAAAG CATTAATCTT CCAAGACCTG ATAATGAAAC TCTCTGGGAT
AAGTTGGACC ATTATTACAG AATTGTCAAG TCAACATTGC TGCTGTATCA AAGTCCAACT
ACCGGTCTCT TTCCCACTAA AACATGCGGT GGTGACCAGA AGGCCAAGAT CCAGGACAGC
CTATACTGCG CTGCTGGGGC CTGGGCTTTG GCTCTTGCAT ACAGGCGAAT TGATGATGAC
AAGGGAAGGA CCCATGAGCT GGAGCACTCA GCTATAAAAT GCATGAGAGG AATTCTCTAC
TGCTATATGC GTCAGGCCGA TAAGGTCCAG CAGTTTAAGC AGGATCCACG CCCAACAACA
TGTCTTCACT CTGTTTTCAA TGTGCATACA GGAGATGAGT TGCTTTCCTA TGAGGAATAT
GGTCATCTTC AGATAAATGC AGTGTCACTT TATCTCCTTT ACCTTGTGGA AATGATTTCC
TCAGGACTCC AGATTATCTA CAACACTGAT GAGGTCTCTT TTATTCAAAA CCTTGTATTT
TGTGTGGAAA GAGTTTACCG TGTGCCTGAC TTTGGTGTCT GGGAAAGAGG AAGCAAATAT
AATAATGGCA GCACAGAGCT ACATTCGAGC TCGGTTGGTT TAGCAAAAGC AGCTCTAGAA
GCAATTAATG GATTCAACCT TTTTGGCAAC CAGGGCTGTT CGTGGTCAGT TATATTTGTG
GATCTCGATG CTCACAATCG CAACAGGCAA ACTTTGTGCT CGCTGTTACC CAGAGAATCA
AGATCACATA ATACAGATGC TGCCCTGCTC CCCTGCATCA GTTATCCTGC ATTTGCCCTG
GATGATGAAG TTCTTTTTAG CCAGACACTT GATAAAGTGG TTAGAAAATT AAAAGGAAAA
TATGGATTTA AACGTTTCTT GAGAGATGGG TATAGAACAT CATTGGAAGA TCCCAACAGA
TGCTACTACA AGCCAGCTGA AATTAAGCTA TTTGATGGCA TTGAATGTGA ATTTCCCATA
TTTTTCCTTT ATATGATGAT TGATGGAGTT TTTAGAGGCA ATCCTAAGCA AGTACAGGAA
TATCAGGATC TTTTGACTCC AGTACTTCAT CATACCACAG AAGGATATCC TGTTGTACCA
AAGTACTATT ATGTGCCAGC TGACTTTGTA GAATATGAAA AAAATAACCC TGGTAGTCAA
AAACGATTTC CTAGCAACTG TGGCCGTGAT GGAAAACTGT TTCTTTGGGG ACAAGCACTT
TATATCATCG CAAAACTCCT GGCTGATGAA CTTATTAGTC CTAAAGACAT TGATCCTGTC
CAGCGCTATG TCCCACTAAA GGATCAACGT AACGTGAGCA TGAGGTTTTC CAATCAGGGC
CCACTGGAAA ATGACTTGGT AGTTCATGTG GCACTTATAG CAGAAAGCCA AAGACTTCAA
GTTTTTCTGA ACACATATGG TATTCAAACT CAAACTCCTC AACAAGTAGA ACCCATTCAG
ATATGGCCTC AGCAGGAGCT TGTGAAAGCT TATTTGCAGC TGGGTATCAA TGAAAAGTTA
GGACTCTCTG GAAGGCCAGA CAGGCCCATT GGCTGCCTCG GGACATCAAA GATTTATCGC
ATTCTAGGAA AGACTGTGGT TTGTTACCCG ATTATTTTCG ACCTAAGTGA TTTCTACATG
TCTCAGGATG TTTTCCTGCT GATAGATGAC ATAAAGAATG CGCTGCAGTT CATTAAACAA
TATTGGAAAA TGCATGGACG TCCACTTTTC CTTGTTCTCA TCCGGGAAGA CAATATAAGA
GGTAGCCGGT TCAACCCCAT ATTAGATATG CTGGCAGCCC TTAAAAAAGG AATAATTGGA
GGAGTCAAAG TTCATGTGGA TCGTCTACAG ACACTAATAT CTGGAGCTGT GGTAGAACAA
CTTGATTTCC TACGAATCAG TGACACAGAA GAGCTTCCAG AATTTAAGAG TTTTGAGGAA
CTAGAACCTC CCAAACATTC AAAAGTCAAA CGGCAAAGCA GCACCCCTAG TGCTCCTGAA
CTGGGACAGC AGCCGGATGT CAACATTAGT GAATGGAAGG ACAAACCCAC CCACGAAATT
CTTCAAAAAC TGAATGATTG CAGTTGTCTG GCTAGCCAAG CCATCCTGCT GGGTATACTG
CTCAAAAGAG AAGGCCCCAA CTTCATCACA AAGGAAGGTA CCGTTTCTGA TCACATTGAG
AGAGTCTATA GAAGAGCTGG CAGCCAAAAA CTTTGGTCGG TTGTACGCCG TGCAGCAAGT
CTTTTAAGTA AAGTAGTGGA CAGCCTGGCC CCATCCATTA CTAATGTTTT AGTGCAGGGC
AAACAGGTAA CTCTGGGTGC CTTTGGGCAT GAAGAAGAAG TTATCTCTAA TCCTTTGTCT
CCAAGAGTGA TTCAAAACAT CATCTATTAT AAGTGTAACA CCCATGATGA GAGGGAAGCG
GTCATTCAGC AAGAACTGGT CATCCATATT GGCTGGATCA TCTCCAATAA CCCTGAGTTA
TTCAGTGGCA TGCTGAAAAT ACGAATCGGG TGGATCATCC ATGCCATGGA GTATGAACTT
CAGATCCGTG GCGGAGACAA GCCAGCCTTG GACTTGTATC AGCTGTCACC TAGTGAAGTT
AAACAGCTTC TGCTGGATAT TCTGCAGCCT CAACAGAATG GAAGATGTTG GCTGAACAGG
CGTCAGATCG ATGGGTCTTT GAATAGAACT CCCACCGGGT TCTATGACCG AGTGTGGCAG
ATTCTGGAGC GCACGCCCAA TGGGATCATT GTTGCTGGGA AGCATTTGCC TCAGCAACCA
ACCCTGTCAG ATATGACCAT GTATGAGATG AATTTCTCTC TCCTTGTTGA AGACACGTTG
GGAAATATTG ACCAGCCACA GTACAGACAG ATCGTTGTAG AGTTACTTAT GGTTGTATCC
ATTGTACTGG AAAGAAACCC CGAGCTAGAA TTTCAAGACA AAGTAGATCT AGACAGACTG
GTCAAAGAAG CATTTAATGA ATTTCAAAAA GATCAGAGTC GGCTAAAGGA AATTGAAAAA
CAAGATGACA TGACTTCCTT TTACAACACT CCTCCCCTGG GAAAAAGAGG AACATGCAGC
TATTTGACAA AGGCGGTGAT GAATCTGCTG CTGGAAGGAG AAGTCAAGCC AAACAATGAT
GACCCGTGTC TGATTAGCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005256041.1
CDS200..3460
Misc Feature(1)323..>1588(+)
Misc Feature(2)2429..2812(+)
Translation

Target ORF information:

RefSeq Version XM_005255984
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phosphorylase kinase, beta (PHKB), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005255984

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGGCCTGCT CACCTGATGC AGTCGTCTCT CCGTCTTCCG CTTTCTTAAG GTCTGGCTCA 
GTTTATGAAC CTCTTAAAAG CATTAATCTT CCAAGACCTG ATAATGAAAC TCTCTGGGAT
AAGTTGGACC ATTATTACAG AATTGTCAAG TCAACATTGC TGCTGTATCA AAGTCCAACT
ACCGGTCTCT TTCCCACTAA AACATGCGGT GGTGACCAGA AGGCCAAGAT CCAGGACAGC
CTATACTGCG CTGCTGGGGC CTGGGCTTTG GCTCTTGCAT ACAGGCGAAT TGATGATGAC
AAGGGAAGGA CCCATGAGCT GGAGCACTCA GCTATAAAAT GCATGAGAGG AATTCTCTAC
TGCTATATGC GTCAGGCCGA TAAGGTCCAG CAGTTTAAGC AGGATCCACG CCCAACAACA
TGTCTTCACT CTGTTTTCAA TGTGCATACA GGAGATGAGT TGCTTTCCTA TGAGGAATAT
GGTCATCTTC AGATAAATGC AGTGTCACTT TATCTCCTTT ACCTTGTGGA AATGATTTCC
TCAGGACTCC AGATTATCTA CAACACTGAT GAGGTCTCTT TTATTCAAAA CCTTGTATTT
TGTGTGGAAA GAGTTTACCG TGTGCCTGAC TTTGGTGTCT GGGAAAGAGG AAGCAAATAT
AATAATGGCA GCACAGAGCT ACATTCGAGC TCGGTTGGTT TAGCAAAAGC AGCTCTAGAA
GCAATTAATG GATTCAACCT TTTTGGCAAC CAGGGCTGTT CGTGGTCAGT TATATTTGTG
GATCTCGATG CTCACAATCG CAACAGGCAA ACTTTGTGCT CGCTGTTACC CAGAGAATCA
AGATCACATA ATACAGATGC TGCCCTGCTC CCCTGCATCA GTTATCCTGC ATTTGCCCTG
GATGATGAAG TTCTTTTTAG CCAGACACTT GATAAAGTGG TTAGAAAATT AAAAGGAAAA
TATGGATTTA AACGTTTCTT GAGAGATGGG TATAGAACAT CATTGGAAGA TCCCAACAGA
TGCTACTACA AGCCAGCTGA AATTAAGCTA TTTGATGGCA TTGAATGTGA ATTTCCCATA
TTTTTCCTTT ATATGATGAT TGATGGAGTT TTTAGAGGCA ATCCTAAGCA AGTACAGGAA
TATCAGGATC TTTTGACTCC AGTACTTCAT CATACCACAG AAGGATATCC TGTTGTACCA
AAGTACTATT ATGTGCCAGC TGACTTTGTA GAATATGAAA AAAATAACCC TGGTAGTCAA
AAACGATTTC CTAGCAACTG TGGCCGTGAT GGAAAACTGT TTCTTTGGGG ACAAGCACTT
TATATCATCG CAAAACTCCT GGCTGATGAA CTTATTAGTC CTAAAGACAT TGATCCTGTC
CAGCGCTATG TCCCACTAAA GGATCAACGT AACGTGAGCA TGAGGTTTTC CAATCAGGGC
CCACTGGAAA ATGACTTGGT AGTTCATGTG GCACTTATAG CAGAAAGCCA AAGACTTCAA
GTTTTTCTGA ACACATATGG TATTCAAACT CAAACTCCTC AACAAGTAGA ACCCATTCAG
ATATGGCCTC AGCAGGAGCT TGTGAAAGCT TATTTGCAGC TGGGTATCAA TGAAAAGTTA
GGACTCTCTG GAAGGCCAGA CAGGCCCATT GGCTGCCTCG GGACATCAAA GATTTATCGC
ATTCTAGGAA AGACTGTGGT TTGTTACCCG ATTATTTTCG ACCTAAGTGA TTTCTACATG
TCTCAGGATG TTTTCCTGCT GATAGATGAC ATAAAGAATG CGCTGCAGTT CATTAAACAA
TATTGGAAAA TGCATGGACG TCCACTTTTC CTTGTTCTCA TCCGGGAAGA CAATATAAGA
GGTAGCCGGT TCAACCCCAT ATTAGATATG CTGGCAGCCC TTAAAAAAGG AATAATTGGA
GGAGTCAAAG TTCATGTGGA TCGTCTACAG ACACTAATAT CTGGAGCTGT GGTAGAACAA
CTTGATTTCC TACGAATCAG TGACACAGAA GAGCTTCCAG AATTTAAGAG TTTTGAGGAA
CTAGAACCTC CCAAACATTC AAAAGTCAAA CGGCAAAGCA GCACCCCTAG TGCTCCTGAA
CTGGGACAGC AGCCGGATGT CAACATTAGT GAATGGAAGG ACAAACCCAC CCACGAAATT
CTTCAAAAAC TGAATGATTG CAGTTGTCTG GCTAGCCAAG CCATCCTGCT GGGTATACTG
CTCAAAAGAG AAGGCCCCAA CTTCATCACA AAGGAAGGTA CCGTTTCTGA TCACATTGAG
AGAGTCTATA GAAGAGCTGG CAGCCAAAAA CTTTGGTCGG TTGTACGCCG TGCAGCAAGT
CTTTTAAGTA AAGTAGTGGA CAGCCTGGCC CCATCCATTA CTAATGTTTT AGTGCAGGGC
AAACAGGTAA CTCTGGGTGC CTTTGGGCAT GAAGAAGAAG TTATCTCTAA TCCTTTGTCT
CCAAGAGTGA TTCAAAACAT CATCTATTAT AAGTGTAACA CCCATGATGA GAGGGAAGCG
GTCATTCAGC AAGAACTGGT CATCCATATT GGCTGGATCA TCTCCAATAA CCCTGAGTTA
TTCAGTGGCA TGCTGAAAAT ACGAATCGGG TGGATCATCC ATGCCATGGA GTATGAACTT
CAGATCCGTG GCGGAGACAA GCCAGCCTTG GACTTGTATC AGCTGTCACC TAGTGAAGTT
AAACAGCTTC TGCTGGATAT TCTGCAGCCT CAACAGAATG GAAGATGTTG GCTGAACAGG
CGTCAGATCG ATGGGTCTTT GAATAGAACT CCCACCGGGT TCTATGACCG AGTGTGGCAG
ATTCTGGAGC GCACGCCCAA TGGGATCATT GTTGCTGGGA AGCATTTGCC TCAGCAACCA
ACCCTGTCAG ATATGACCAT GTATGAGATG AATTTCTCTC TCCTTGTTGA AGACACGTTG
GGAAATATTG ACCAGCCACA GTACAGACAG ATCGTTGTAG AGTTACTTAT GGTTGTATCC
ATTGTACTGG AAAGAAACCC CGAGCTAGAA TTTCAAGACA AAGTAGATCT AGACAGACTG
GTCAAAGAAG CATTTAATGA ATTTCAAAAA GATCAGAGTC GGCTAAAGGA AATTGAAAAA
CAAGATGACA TGACTTCCTT TTACAACACT CCTCCCCTGG GAAAAAGAGG AACATGCAGC
TATTTGACAA AGGCGGTGAT GAATCTGCTG CTGGAAGGAG AAGTCAAGCC AAACAATGAT
GACCCGTGTC TGATTAGCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59673
Clone ID Related Accession (Same CDS sequence) XM_011523106
Accession Version XM_011523106.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2340bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product phosphorylase b kinase regulatory subunit beta isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010498.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCGGGGG CGGCGGGACT CACGGCAGAA GTGAGCTGGA AGGTCTTGGA GCGAAGAGCT 
CGGACCAAGC GCTCAGGCTC AGTTTATGAA CCTCTTAAAA GCATTAATCT TCCAAGACCT
GATAATGAAA CTCTCTGGGA TAAGTTGGAC CATTATTACA GAATTGTCAA GTCAACATTG
CTGCTGTATC AAAGTCCAAC TACCGGTCTC TTTCCCACTA AAACATGCGG TGGTGACCAG
AAGGCCAAGA TCCAGGACAG CCTATACTGC GCTGCTGGGG CCTGGGCTTT GGCTCTTGCA
TACAGGCGAA TTGATGATGA CAAGGGAAGG ACCCATGAGC TGGAGCACTC AGCTATAAAA
TGCATGAGAG GAATTCTCTA CTGCTATATG CGTCAGGCCG ATAAGGTCCA GCAGTTTAAG
CAGGATCCAC GCCCAACAAC ATGTCTTCAC TCTGTTTTCA ATGTGCATAC AGGAGATGAG
TTGCTTTCCT ATGAGGAATA TGGTCATCTT CAGATAAATG CAGTGTCACT TTATCTCCTT
TACCTTGTGG AAATGATTTC CTCAGGACTC CAGATTATCT ACAACACTGA TGAGGTCTCT
TTTATTCAAA ACCTTGTATT TTGTGTGGAA AGAGTTTACC GTGTGCCTGA CTTTGGTGTC
TGGGAAAGAG GAAGCAAATA TAATAATGGC AGCACAGAGC TACATTCGAG CTCGGTTGGT
TTAGCAAAAG CAGCTCTAGA AGCAATTAAT GGATTCAACC TTTTTGGCAA CCAGGGCTGT
TCGTGGTCAG TTATATTTGT GGATCTCGAT GCTCACAATC GCAACAGGCA AACTTTGTGC
TCGCTGTTAC CCAGAGAATC AAGATCACAT AATACAGATG CTGCCCTGCT CCCCTGCATC
AGTTATCCTG CATTTGCCCT GGATGATGAA GTTCTTTTTA GCCAGACACT TGATAAAGTG
GTTAGAAAAT TAAAAGGAAA ATATGGATTT AAACGTTTCT TGAGAGATGG GTATAGAACA
TCATTGGAAG ATCCCAACAG ATGCTACTAC AAGCCAGCTG AAATTAAGCT ATTTGATGGC
ATTGAATGTG AATTTCCCAT ATTTTTCCTT TATATGATGA TTGATGGAGT TTTTAGAGGC
AATCCTAAGC AAGTACAGGA ATATCAGGAT CTTTTGACTC CAGTACTTCA TCATACCACA
GAAGGATATC CTGTTGTACC AAAGTACTAT TATGTGCCAG CTGACTTTGT AGAATATGAA
AAAAATAACC CTGGTAGTCA AAAACGATTT CCTAGCAACT GTGGCCGTGA TGGAAAACTG
TTTCTTTGGG GACAAGCACT TTATATCATC GCAAAACTCC TGGCTGATGA ACTTATTAGT
CCTAAAGACA TTGATCCTGT CCAGCGCTAT GTCCCACTAA AGGATCAACG TAACGTGAGC
ATGAGGTTTT CCAATCAGGG CCCACTGGAA AATGACTTGG TAGTTCATGT GGCACTTATA
GCAGAAAGCC AAAGACTTCA AGTTTTTCTG AACACATATG GTATTCAAAC TCAAACTCCT
CAACAAGTAG AACCCATTCA GATATGGCCT CAGCAGGAGC TTGTGAAAGC TTATTTGCAG
CTGGGTATCA ATGAAAAGTT AGGACTCTCT GGAAGGCCAG ACAGGCCCAT TGGCTGCCTC
GGGACATCAA AGATTTATCG CATTCTAGGA AAGACTGTGG TTTGTTACCC GATTATTTTC
GACCTAAGTG ATTTCTACAT GTCTCAGGAT GTTTTCCTGC TGATAGATGA CATAAAGAAT
GCGCTGCAGT TCATTAAACA ATATTGGAAA ATGCATGGAC GTCCACTTTT CCTTGTTCTC
ATCCGGGAAG ACAATATAAG AGGTAGCCGG TTCAACCCCA TATTAGATAT GCTGGCAGCC
CTTAAAAAAG GAATAATTGG AGGAGTCAAA GTTCATGTGG ATCGTCTACA GACACTAATA
TCTGGAGCTG TGGTAGAACA ACTTGATTTC CTACGAATCA GTGACACAGA AGAGCTTCCA
GAATTTAAGA GTTTTGAGGA ACTAGAACCT CCCAAACATT CAAAAGTCAA ACGGCAAAGC
AGCACCCCTA GTGCTCCTGA ACTGGGACAG CAGCCGGATG TCAACATTAG TGAATGGAAG
GACAAACCCA CCCACGAAAT TCTTCAAAAA CTGAATGATT GCAGTTGTCT GGCTAGCCAA
GCCATCCTGC TGGGTATACT GCTCAAAAGA GAAGGCCCCA ACTTCATCAC AAAGGAAGGT
ACCGTTTCTG ATCACATTGA GAGAGTCTAT AGAAGAGCTG GCAGCCAAAA ACTTTGGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011521408.1
CDS16..2355
Misc Feature(1)160..>1425(+)
Translation

Target ORF information:

RefSeq Version XM_011523106
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phosphorylase kinase, beta (PHKB), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011523106

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCGGGGG CGGCGGGACT CACGGCAGAA GTGAGCTGGA AGGTCTTGGA GCGAAGAGCT 
CGGACCAAGC GCTCAGGCTC AGTTTATGAA CCTCTTAAAA GCATTAATCT TCCAAGACCT
GATAATGAAA CTCTCTGGGA TAAGTTGGAC CATTATTACA GAATTGTCAA GTCAACATTG
CTGCTGTATC AAAGTCCAAC TACCGGTCTC TTTCCCACTA AAACATGCGG TGGTGACCAG
AAGGCCAAGA TCCAGGACAG CCTATACTGC GCTGCTGGGG CCTGGGCTTT GGCTCTTGCA
TACAGGCGAA TTGATGATGA CAAGGGAAGG ACCCATGAGC TGGAGCACTC AGCTATAAAA
TGCATGAGAG GAATTCTCTA CTGCTATATG CGTCAGGCCG ATAAGGTCCA GCAGTTTAAG
CAGGATCCAC GCCCAACAAC ATGTCTTCAC TCTGTTTTCA ATGTGCATAC AGGAGATGAG
TTGCTTTCCT ATGAGGAATA TGGTCATCTT CAGATAAATG CAGTGTCACT TTATCTCCTT
TACCTTGTGG AAATGATTTC CTCAGGACTC CAGATTATCT ACAACACTGA TGAGGTCTCT
TTTATTCAAA ACCTTGTATT TTGTGTGGAA AGAGTTTACC GTGTGCCTGA CTTTGGTGTC
TGGGAAAGAG GAAGCAAATA TAATAATGGC AGCACAGAGC TACATTCGAG CTCGGTTGGT
TTAGCAAAAG CAGCTCTAGA AGCAATTAAT GGATTCAACC TTTTTGGCAA CCAGGGCTGT
TCGTGGTCAG TTATATTTGT GGATCTCGAT GCTCACAATC GCAACAGGCA AACTTTGTGC
TCGCTGTTAC CCAGAGAATC AAGATCACAT AATACAGATG CTGCCCTGCT CCCCTGCATC
AGTTATCCTG CATTTGCCCT GGATGATGAA GTTCTTTTTA GCCAGACACT TGATAAAGTG
GTTAGAAAAT TAAAAGGAAA ATATGGATTT AAACGTTTCT TGAGAGATGG GTATAGAACA
TCATTGGAAG ATCCCAACAG ATGCTACTAC AAGCCAGCTG AAATTAAGCT ATTTGATGGC
ATTGAATGTG AATTTCCCAT ATTTTTCCTT TATATGATGA TTGATGGAGT TTTTAGAGGC
AATCCTAAGC AAGTACAGGA ATATCAGGAT CTTTTGACTC CAGTACTTCA TCATACCACA
GAAGGATATC CTGTTGTACC AAAGTACTAT TATGTGCCAG CTGACTTTGT AGAATATGAA
AAAAATAACC CTGGTAGTCA AAAACGATTT CCTAGCAACT GTGGCCGTGA TGGAAAACTG
TTTCTTTGGG GACAAGCACT TTATATCATC GCAAAACTCC TGGCTGATGA ACTTATTAGT
CCTAAAGACA TTGATCCTGT CCAGCGCTAT GTCCCACTAA AGGATCAACG TAACGTGAGC
ATGAGGTTTT CCAATCAGGG CCCACTGGAA AATGACTTGG TAGTTCATGT GGCACTTATA
GCAGAAAGCC AAAGACTTCA AGTTTTTCTG AACACATATG GTATTCAAAC TCAAACTCCT
CAACAAGTAG AACCCATTCA GATATGGCCT CAGCAGGAGC TTGTGAAAGC TTATTTGCAG
CTGGGTATCA ATGAAAAGTT AGGACTCTCT GGAAGGCCAG ACAGGCCCAT TGGCTGCCTC
GGGACATCAA AGATTTATCG CATTCTAGGA AAGACTGTGG TTTGTTACCC GATTATTTTC
GACCTAAGTG ATTTCTACAT GTCTCAGGAT GTTTTCCTGC TGATAGATGA CATAAAGAAT
GCGCTGCAGT TCATTAAACA ATATTGGAAA ATGCATGGAC GTCCACTTTT CCTTGTTCTC
ATCCGGGAAG ACAATATAAG AGGTAGCCGG TTCAACCCCA TATTAGATAT GCTGGCAGCC
CTTAAAAAAG GAATAATTGG AGGAGTCAAA GTTCATGTGG ATCGTCTACA GACACTAATA
TCTGGAGCTG TGGTAGAACA ACTTGATTTC CTACGAATCA GTGACACAGA AGAGCTTCCA
GAATTTAAGA GTTTTGAGGA ACTAGAACCT CCCAAACATT CAAAAGTCAA ACGGCAAAGC
AGCACCCCTA GTGCTCCTGA ACTGGGACAG CAGCCGGATG TCAACATTAG TGAATGGAAG
GACAAACCCA CCCACGAAAT TCTTCAAAAA CTGAATGATT GCAGTTGTCT GGCTAGCCAA
GCCATCCTGC TGGGTATACT GCTCAAAAGA GAAGGCCCCA ACTTCATCAC AAAGGAAGGT
ACCGTTTCTG ATCACATTGA GAGAGTCTAT AGAAGAGCTG GCAGCCAAAA ACTTTGGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59674
Clone ID Related Accession (Same CDS sequence) XM_011523107
Accession Version XM_011523107.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1860bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product phosphorylase b kinase regulatory subunit beta isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010498.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGAACCTGA CCATAAATCG CAGACAATGG GCAAAGGGCC CACTGGAAAA TGACTTGGTA 
GTTCATGTGG CACTTATAGC AGAAAGCCAA AGACTTCAAG TTTTTCTGAA CACATATGGT
ATTCAAACTC AAACTCCTCA ACAAGTAGAA CCCATTCAGA TATGGCCTCA GCAGGAGCTT
GTGAAAGCTT ATTTGCAGCT GGGTATCAAT GAAAAGTTAG GACTCTCTGG AAGGCCAGAC
AGGCCCATTG GCTGCCTCGG GACATCAAAG ATTTATCGCA TTCTAGGAAA GACTGTGGTT
TGTTACCCGA TTATTTTCGA CCTAAGTGAT TTCTACATGT CTCAGGATGT TTTCCTGCTG
ATAGATGACA TAAAGAATGC GCTGCAGTTC ATTAAACAAT ATTGGAAAAT GCATGGACGT
CCACTTTTCC TTGTTCTCAT CCGGGAAGAC AATATAAGAG GTAGCCGGTT CAACCCCATA
TTAGATATGC TGGCAGCCCT TAAAAAAGGA ATAATTGGAG GAGTCAAAGT TCATGTGGAT
CGTCTACAGA CACTAATATC TGGAGCTGTG GTAGAACAAC TTGATTTCCT ACGAATCAGT
GACACAGAAG AGCTTCCAGA ATTTAAGAGT TTTGAGGAAC TAGAACCTCC CAAACATTCA
AAAGTCAAAC GGCAAAGCAG CACCCCTAGT GCTCCTGAAC TGGGACAGCA GCCGGATGTC
AACATTAGTG AATGGAAGGA CAAACCCACC CACGAAATTC TTCAAAAACT GAATGATTGC
AGTTGTCTGG CTAGCCAAGC CATCCTGCTG GGTATACTGC TCAAAAGAGA AGGCCCCAAC
TTCATCACAA AGGAAGGTAC CGTTTCTGAT CACATTGAGA GAGTCTATAG AAGAGCTGGC
AGCCAAAAAC TTTGGTCGGT TGTACGCCGT GCAGCAAGTC TTTTAAGTAA AGTAGTGGAC
AGCCTGGCCC CATCCATTAC TAATGTTTTA GTGCAGGGCA AACAGGTAAC TCTGGGTGCC
TTTGGGCATG AAGAAGAAGT TATCTCTAAT CCTTTGTCTC CAAGAGTGAT TCAAAACATC
ATCTATTATA AGTGTAACAC CCATGATGAG AGGGAAGCGG TCATTCAGCA AGAACTGGTC
ATCCATATTG GCTGGATCAT CTCCAATAAC CCTGAGTTAT TCAGTGGCAT GCTGAAAATA
CGAATCGGGT GGATCATCCA TGCCATGGAG TATGAACTTC AGATCCGTGG CGGAGACAAG
CCAGCCTTGG ACTTGTATCA GCTGTCACCT AGTGAAGTTA AACAGCTTCT GCTGGATATT
CTGCAGCCTC AACAGAATGG AAGATGTTGG CTGAACAGGC GTCAGATCGA TGGGTCTTTG
AATAGAACTC CCACCGGGTT CTATGACCGA GTGTGGCAGA TTCTGGAGCG CACGCCCAAT
GGGATCATTG TTGCTGGGAA GCATTTGCCT CAGCAACCAA CCCTGTCAGA TATGACCATG
TATGAGATGA ATTTCTCTCT CCTTGTTGAA GACACGTTGG GAAATATTGA CCAGCCACAG
TACAGACAGA TCGTTGTAGA GTTACTTATG GTTGTATCCA TTGTACTGGA AAGAAACCCC
GAGCTAGAAT TTCAAGACAA AGTAGATCTA GACAGACTGG TCAAAGAAGC ATTTAATGAA
TTTCAAAAAG ATCAGAGTCG GCTAAAGGAA ATTGAAAAAC AAGATGACAT GACTTCCTTT
TACAACACTC CTCCCCTGGG AAAAAGAGGA ACATGCAGCT ATTTGACAAA GGCGGTGATG
AATCTGCTGC TGGAAGGAGA AGTCAAGCCA AACAATGATG ACCCGTGTCT GATTAGCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011521409.1
CDS125..1984
Misc Feature(1)953..1336(+)
Translation

Target ORF information:

RefSeq Version XM_011523107
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phosphorylase kinase, beta (PHKB), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011523107

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGAACCTGA CCATAAATCG CAGACAATGG GCAAAGGGCC CACTGGAAAA TGACTTGGTA 
GTTCATGTGG CACTTATAGC AGAAAGCCAA AGACTTCAAG TTTTTCTGAA CACATATGGT
ATTCAAACTC AAACTCCTCA ACAAGTAGAA CCCATTCAGA TATGGCCTCA GCAGGAGCTT
GTGAAAGCTT ATTTGCAGCT GGGTATCAAT GAAAAGTTAG GACTCTCTGG AAGGCCAGAC
AGGCCCATTG GCTGCCTCGG GACATCAAAG ATTTATCGCA TTCTAGGAAA GACTGTGGTT
TGTTACCCGA TTATTTTCGA CCTAAGTGAT TTCTACATGT CTCAGGATGT TTTCCTGCTG
ATAGATGACA TAAAGAATGC GCTGCAGTTC ATTAAACAAT ATTGGAAAAT GCATGGACGT
CCACTTTTCC TTGTTCTCAT CCGGGAAGAC AATATAAGAG GTAGCCGGTT CAACCCCATA
TTAGATATGC TGGCAGCCCT TAAAAAAGGA ATAATTGGAG GAGTCAAAGT TCATGTGGAT
CGTCTACAGA CACTAATATC TGGAGCTGTG GTAGAACAAC TTGATTTCCT ACGAATCAGT
GACACAGAAG AGCTTCCAGA ATTTAAGAGT TTTGAGGAAC TAGAACCTCC CAAACATTCA
AAAGTCAAAC GGCAAAGCAG CACCCCTAGT GCTCCTGAAC TGGGACAGCA GCCGGATGTC
AACATTAGTG AATGGAAGGA CAAACCCACC CACGAAATTC TTCAAAAACT GAATGATTGC
AGTTGTCTGG CTAGCCAAGC CATCCTGCTG GGTATACTGC TCAAAAGAGA AGGCCCCAAC
TTCATCACAA AGGAAGGTAC CGTTTCTGAT CACATTGAGA GAGTCTATAG AAGAGCTGGC
AGCCAAAAAC TTTGGTCGGT TGTACGCCGT GCAGCAAGTC TTTTAAGTAA AGTAGTGGAC
AGCCTGGCCC CATCCATTAC TAATGTTTTA GTGCAGGGCA AACAGGTAAC TCTGGGTGCC
TTTGGGCATG AAGAAGAAGT TATCTCTAAT CCTTTGTCTC CAAGAGTGAT TCAAAACATC
ATCTATTATA AGTGTAACAC CCATGATGAG AGGGAAGCGG TCATTCAGCA AGAACTGGTC
ATCCATATTG GCTGGATCAT CTCCAATAAC CCTGAGTTAT TCAGTGGCAT GCTGAAAATA
CGAATCGGGT GGATCATCCA TGCCATGGAG TATGAACTTC AGATCCGTGG CGGAGACAAG
CCAGCCTTGG ACTTGTATCA GCTGTCACCT AGTGAAGTTA AACAGCTTCT GCTGGATATT
CTGCAGCCTC AACAGAATGG AAGATGTTGG CTGAACAGGC GTCAGATCGA TGGGTCTTTG
AATAGAACTC CCACCGGGTT CTATGACCGA GTGTGGCAGA TTCTGGAGCG CACGCCCAAT
GGGATCATTG TTGCTGGGAA GCATTTGCCT CAGCAACCAA CCCTGTCAGA TATGACCATG
TATGAGATGA ATTTCTCTCT CCTTGTTGAA GACACGTTGG GAAATATTGA CCAGCCACAG
TACAGACAGA TCGTTGTAGA GTTACTTATG GTTGTATCCA TTGTACTGGA AAGAAACCCC
GAGCTAGAAT TTCAAGACAA AGTAGATCTA GACAGACTGG TCAAAGAAGC ATTTAATGAA
TTTCAAAAAG ATCAGAGTCG GCTAAAGGAA ATTGAAAAAC AAGATGACAT GACTTCCTTT
TACAACACTC CTCCCCTGGG AAAAAGAGGA ACATGCAGCT ATTTGACAAA GGCGGTGATG
AATCTGCTGC TGGAAGGAGA AGTCAAGCCA AACAATGATG ACCCGTGTCT GATTAGCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18700
Clone ID Related Accession (Same CDS sequence) NM_000293
Accession Version NM_000293.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3282bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product phosphorylase b kinase regulatory subunit beta isoform a
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC007599.6, X84908.1 and AC092374.4. This sequence is a reference standard in the RefSeqGene project. On Mar 13, 2008 this sequence version replaced gi:4505782. Transcript Variant: This variant (1) encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X84908.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2146411, SAMEA2151741 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGGCGGGGG CGGCGGGACT CACGGCAGAA GTGAGCTGGA AGGTCTTGGA GCGAAGAGCT 
CGGACCAAGC GCTCAGGCTC AGTTTATGAA CCTCTTAAAA GCATTAATCT TCCAAGACCT
GATAATGAAA CTCTCTGGGA TAAGTTGGAC CATTATTACA GAATTGTCAA GTCAACATTG
CTGCTGTATC AAAGTCCAAC TACCGGTCTC TTTCCCACTA AAACATGCGG TGGTGACCAG
AAGGCCAAGA TCCAGGACAG CCTATACTGC GCTGCTGGGG CCTGGGCTTT GGCTCTTGCA
TACAGGCGAA TTGATGATGA CAAGGGAAGG ACCCATGAGC TGGAGCACTC AGCTATAAAA
TGCATGAGAG GAATTCTCTA CTGCTATATG CGTCAGGCCG ATAAGGTCCA GCAGTTTAAG
CAGGATCCAC GCCCAACAAC ATGTCTTCAC TCTGTTTTCA ATGTGCATAC AGGAGATGAG
TTGCTTTCCT ATGAGGAATA TGGTCATCTT CAGATAAATG CAGTGTCACT TTATCTCCTT
TACCTTGTGG AAATGATTTC CTCAGGACTC CAGATTATCT ACAACACTGA TGAGGTCTCT
TTTATTCAAA ACCTTGTATT TTGTGTGGAA AGAGTTTACC GTGTGCCTGA CTTTGGTGTC
TGGGAAAGAG GAAGCAAATA TAATAATGGC AGCACAGAGC TACATTCGAG CTCGGTTGGT
TTAGCAAAAG CAGCTCTAGA AGCAATTAAT GGATTCAACC TTTTTGGCAA CCAGGGCTGT
TCGTGGTCAG TTATATTTGT GGATCTCGAT GCTCACAATC GCAACAGGCA AACTTTGTGC
TCGCTGTTAC CCAGAGAATC AAGATCACAT AATACAGATG CTGCCCTGCT CCCCTGCATC
AGTTATCCTG CATTTGCCCT GGATGATGAA GTTCTTTTTA GCCAGACACT TGATAAAGTG
GTTAGAAAAT TAAAAGGAAA ATATGGATTT AAACGTTTCT TGAGAGATGG GTATAGAACA
TCATTGGAAG ATCCCAACAG ATGCTACTAC AAGCCAGCTG AAATTAAGCT ATTTGATGGC
ATTGAATGTG AATTTCCCAT ATTTTTCCTT TATATGATGA TTGATGGAGT TTTTAGAGGC
AATCCTAAGC AAGTACAGGA ATATCAGGAT CTTTTGACTC CAGTACTTCA TCATACCACA
GAAGGATATC CTGTTGTACC AAAGTACTAT TATGTGCCAG CTGACTTTGT AGAATATGAA
AAAAATAACC CTGGTAGTCA AAAACGATTT CCTAGCAACT GTGGCCGTGA TGGAAAACTG
TTTCTTTGGG GACAAGCACT TTATATCATC GCAAAACTCC TGGCTGATGA ACTTATTAGT
CCTAAAGACA TTGATCCTGT CCAGCGCTAT GTCCCACTAA AGGATCAACG TAACGTGAGC
ATGAGGTTTT CCAATCAGGG CCCACTGGAA AATGACTTGG TAGTTCATGT GGCACTTATA
GCAGAAAGCC AAAGACTTCA AGTTTTTCTG AACACATATG GTATTCAAAC TCAAACTCCT
CAACAAGTAG AACCCATTCA GATATGGCCT CAGCAGGAGC TTGTGAAAGC TTATTTGCAG
CTGGGTATCA ATGAAAAGTT AGGACTCTCT GGAAGGCCAG ACAGGCCCAT TGGCTGCCTC
GGGACATCAA AGATTTATCG CATTCTAGGA AAGACTGTGG TTTGTTACCC GATTATTTTC
GACCTAAGTG ATTTCTACAT GTCTCAGGAT GTTTTCCTGC TGATAGATGA CATAAAGAAT
GCGCTGCAGT TCATTAAACA ATATTGGAAA ATGCATGGAC GTCCACTTTT CCTTGTTCTC
ATCCGGGAAG ACAATATAAG AGGTAGCCGG TTCAACCCCA TATTAGATAT GCTGGCAGCC
CTTAAAAAAG GAATAATTGG AGGAGTCAAA GTTCATGTGG ATCGTCTACA GACACTAATA
TCTGGAGCTG TGGTAGAACA ACTTGATTTC CTACGAATCA GTGACACAGA AGAGCTTCCA
GAATTTAAGA GTTTTGAGGA ACTAGAACCT CCCAAACATT CAAAAGTCAA ACGGCAAAGC
AGCACCCCTA GTGCTCCTGA ACTGGGACAG CAGCCGGATG TCAACATTAG TGAATGGAAG
GACAAACCCA CCCACGAAAT TCTTCAAAAA CTGAATGATT GCAGTTGTCT GGCTAGCCAA
GCCATCCTGC TGGGTATACT GCTCAAAAGA GAAGGCCCCA ACTTCATCAC AAAGGAAGGT
ACCGTTTCTG ATCACATTGA GAGAGTCTAT AGAAGAGCTG GCAGCCAAAA ACTTTGGTTG
GCGGTGCGCT ACGGGGCTGC ATTTACCCAG AAATTTTCTT CCTCTATAGC CCCACACATT
ACTACTTTTC TGGTACATGG GAAACAGGTA ACTCTGGGTG CCTTTGGGCA TGAAGAAGAA
GTTATCTCTA ATCCTTTGTC TCCAAGAGTG ATTCAAAACA TCATCTATTA TAAGTGTAAC
ACCCATGATG AGAGGGAAGC GGTCATTCAG CAAGAACTGG TCATCCATAT TGGCTGGATC
ATCTCCAATA ACCCTGAGTT ATTCAGTGGC ATGCTGAAAA TACGAATCGG GTGGATCATC
CATGCCATGG AGTATGAACT TCAGATCCGT GGCGGAGACA AGCCAGCCTT GGACTTGTAT
CAGCTGTCAC CTAGTGAAGT TAAACAGCTT CTGCTGGATA TTCTGCAGCC TCAACAGAAT
GGAAGATGTT GGCTGAACAG GCGTCAGATC GATGGGTCTT TGAATAGAAC TCCCACCGGG
TTCTATGACC GAGTGTGGCA GATTCTGGAG CGCACGCCCA ATGGGATCAT TGTTGCTGGG
AAGCATTTGC CTCAGCAACC AACCCTGTCA GATATGACCA TGTATGAGAT GAATTTCTCT
CTCCTTGTTG AAGACACGTT GGGAAATATT GACCAGCCAC AGTACAGACA GATCGTTGTA
GAGTTACTTA TGGTTGTATC CATTGTACTG GAAAGAAACC CCGAGCTAGA ATTTCAAGAC
AAAGTAGATC TAGACAGACT GGTCAAAGAA GCATTTAATG AATTTCAAAA AGATCAGAGT
CGGCTAAAGG AAATTGAAAA ACAAGATGAC ATGACTTCCT TTTACAACAC TCCTCCCCTG
GGAAAAAGAG GAACATGCAG CTATTTGACA AAGGCGGTGA TGAATCTGCT GCTGGAAGGA
GAAGTCAAGC CAAACAATGA TGACCCGTGT CTGATTAGCT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000284.1
CDS53..3334
Misc Feature(1)71..139(+)
Misc Feature(2)131..133(+)
Misc Feature(3)197..>1462(+)
Misc Feature(4)2303..2686(+)
Misc Feature(5)2354..2437(+)
Misc Feature(6)2810..2905(+)
Exon (1)1..128
Gene:PHKB
Gene Synonym:
Exon (2)129..218
Gene:PHKB
Gene Synonym:
Exon (3)219..357
Gene:PHKB
Gene Synonym:
Exon (4)358..457
Gene:PHKB
Gene Synonym:
Exon (5)458..565
Gene:PHKB
Gene Synonym:
Exon (6)566..646
Gene:PHKB
Gene Synonym:
Exon (7)647..762
Gene:PHKB
Gene Synonym:
Exon (8)763..826
Gene:PHKB
Gene Synonym:
Exon (9)827..922
Gene:PHKB
Gene Synonym:
Exon (10)923..1120
Gene:PHKB
Gene Synonym:
Exon (11)1121..1178
Gene:PHKB
Gene Synonym:
Exon (12)1179..1256
Gene:PHKB
Gene Synonym:
Exon (13)1257..1415
Gene:PHKB
Gene Synonym:
Exon (14)1416..1510
Gene:PHKB
Gene Synonym:
Exon (15)1511..1566
Gene:PHKB
Gene Synonym:
Exon (16)1567..1660
Gene:PHKB
Gene Synonym:
Exon (17)1661..1744
Gene:PHKB
Gene Synonym:
Exon (18)1745..1849
Gene:PHKB
Gene Synonym:
Exon (19)1850..1932
Gene:PHKB
Gene Synonym:
Exon (20)1933..2023
Gene:PHKB
Gene Synonym:
Exon (21)2024..2085
Gene:PHKB
Gene Synonym:
Exon (22)2086..2248
Gene:PHKB
Gene Synonym:
Exon (23)2249..2330
Gene:PHKB
Gene Synonym:
Exon (24)2331..2388
Gene:PHKB
Gene Synonym:
Exon (25)2389..2479
Gene:PHKB
Gene Synonym:
Exon (26)2480..2682
Gene:PHKB
Gene Synonym:
Exon (27)2683..2817
Gene:PHKB
Gene Synonym:
Exon (28)2818..2947
Gene:PHKB
Gene Synonym:
Exon (29)2948..3055
Gene:PHKB
Gene Synonym:
Exon (30)3056..3196
Gene:PHKB
Gene Synonym:
Exon (31)3197..5491
Gene:PHKB
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000293
Organism Homo sapiens (human)
Definition Homo sapiens phosphorylase kinase, beta (PHKB), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000293

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGGCGGGGG CGGCGGGACT CACGGCAGAA GTGAGCTGGA AGGTCTTGGA GCGAAGAGCT 
CGGACCAAGC GCTCAGGCTC AGTTTATGAA CCTCTTAAAA GCATTAATCT TCCAAGACCT
GATAATGAAA CTCTCTGGGA TAAGTTGGAC CATTATTACA GAATTGTCAA GTCAACATTG
CTGCTGTATC AAAGTCCAAC TACCGGTCTC TTTCCCACTA AAACATGCGG TGGTGACCAG
AAGGCCAAGA TCCAGGACAG CCTATACTGC GCTGCTGGGG CCTGGGCTTT GGCTCTTGCA
TACAGGCGAA TTGATGATGA CAAGGGAAGG ACCCATGAGC TGGAGCACTC AGCTATAAAA
TGCATGAGAG GAATTCTCTA CTGCTATATG CGTCAGGCCG ATAAGGTCCA GCAGTTTAAG
CAGGATCCAC GCCCAACAAC ATGTCTTCAC TCTGTTTTCA ATGTGCATAC AGGAGATGAG
TTGCTTTCCT ATGAGGAATA TGGTCATCTT CAGATAAATG CAGTGTCACT TTATCTCCTT
TACCTTGTGG AAATGATTTC CTCAGGACTC CAGATTATCT ACAACACTGA TGAGGTCTCT
TTTATTCAAA ACCTTGTATT TTGTGTGGAA AGAGTTTACC GTGTGCCTGA CTTTGGTGTC
TGGGAAAGAG GAAGCAAATA TAATAATGGC AGCACAGAGC TACATTCGAG CTCGGTTGGT
TTAGCAAAAG CAGCTCTAGA AGCAATTAAT GGATTCAACC TTTTTGGCAA CCAGGGCTGT
TCGTGGTCAG TTATATTTGT GGATCTCGAT GCTCACAATC GCAACAGGCA AACTTTGTGC
TCGCTGTTAC CCAGAGAATC AAGATCACAT AATACAGATG CTGCCCTGCT CCCCTGCATC
AGTTATCCTG CATTTGCCCT GGATGATGAA GTTCTTTTTA GCCAGACACT TGATAAAGTG
GTTAGAAAAT TAAAAGGAAA ATATGGATTT AAACGTTTCT TGAGAGATGG GTATAGAACA
TCATTGGAAG ATCCCAACAG ATGCTACTAC AAGCCAGCTG AAATTAAGCT ATTTGATGGC
ATTGAATGTG AATTTCCCAT ATTTTTCCTT TATATGATGA TTGATGGAGT TTTTAGAGGC
AATCCTAAGC AAGTACAGGA ATATCAGGAT CTTTTGACTC CAGTACTTCA TCATACCACA
GAAGGATATC CTGTTGTACC AAAGTACTAT TATGTGCCAG CTGACTTTGT AGAATATGAA
AAAAATAACC CTGGTAGTCA AAAACGATTT CCTAGCAACT GTGGCCGTGA TGGAAAACTG
TTTCTTTGGG GACAAGCACT TTATATCATC GCAAAACTCC TGGCTGATGA ACTTATTAGT
CCTAAAGACA TTGATCCTGT CCAGCGCTAT GTCCCACTAA AGGATCAACG TAACGTGAGC
ATGAGGTTTT CCAATCAGGG CCCACTGGAA AATGACTTGG TAGTTCATGT GGCACTTATA
GCAGAAAGCC AAAGACTTCA AGTTTTTCTG AACACATATG GTATTCAAAC TCAAACTCCT
CAACAAGTAG AACCCATTCA GATATGGCCT CAGCAGGAGC TTGTGAAAGC TTATTTGCAG
CTGGGTATCA ATGAAAAGTT AGGACTCTCT GGAAGGCCAG ACAGGCCCAT TGGCTGCCTC
GGGACATCAA AGATTTATCG CATTCTAGGA AAGACTGTGG TTTGTTACCC GATTATTTTC
GACCTAAGTG ATTTCTACAT GTCTCAGGAT GTTTTCCTGC TGATAGATGA CATAAAGAAT
GCGCTGCAGT TCATTAAACA ATATTGGAAA ATGCATGGAC GTCCACTTTT CCTTGTTCTC
ATCCGGGAAG ACAATATAAG AGGTAGCCGG TTCAACCCCA TATTAGATAT GCTGGCAGCC
CTTAAAAAAG GAATAATTGG AGGAGTCAAA GTTCATGTGG ATCGTCTACA GACACTAATA
TCTGGAGCTG TGGTAGAACA ACTTGATTTC CTACGAATCA GTGACACAGA AGAGCTTCCA
GAATTTAAGA GTTTTGAGGA ACTAGAACCT CCCAAACATT CAAAAGTCAA ACGGCAAAGC
AGCACCCCTA GTGCTCCTGA ACTGGGACAG CAGCCGGATG TCAACATTAG TGAATGGAAG
GACAAACCCA CCCACGAAAT TCTTCAAAAA CTGAATGATT GCAGTTGTCT GGCTAGCCAA
GCCATCCTGC TGGGTATACT GCTCAAAAGA GAAGGCCCCA ACTTCATCAC AAAGGAAGGT
ACCGTTTCTG ATCACATTGA GAGAGTCTAT AGAAGAGCTG GCAGCCAAAA ACTTTGGTTG
GCGGTGCGCT ACGGGGCTGC ATTTACCCAG AAATTTTCTT CCTCTATAGC CCCACACATT
ACTACTTTTC TGGTACATGG GAAACAGGTA ACTCTGGGTG CCTTTGGGCA TGAAGAAGAA
GTTATCTCTA ATCCTTTGTC TCCAAGAGTG ATTCAAAACA TCATCTATTA TAAGTGTAAC
ACCCATGATG AGAGGGAAGC GGTCATTCAG CAAGAACTGG TCATCCATAT TGGCTGGATC
ATCTCCAATA ACCCTGAGTT ATTCAGTGGC ATGCTGAAAA TACGAATCGG GTGGATCATC
CATGCCATGG AGTATGAACT TCAGATCCGT GGCGGAGACA AGCCAGCCTT GGACTTGTAT
CAGCTGTCAC CTAGTGAAGT TAAACAGCTT CTGCTGGATA TTCTGCAGCC TCAACAGAAT
GGAAGATGTT GGCTGAACAG GCGTCAGATC GATGGGTCTT TGAATAGAAC TCCCACCGGG
TTCTATGACC GAGTGTGGCA GATTCTGGAG CGCACGCCCA ATGGGATCAT TGTTGCTGGG
AAGCATTTGC CTCAGCAACC AACCCTGTCA GATATGACCA TGTATGAGAT GAATTTCTCT
CTCCTTGTTG AAGACACGTT GGGAAATATT GACCAGCCAC AGTACAGACA GATCGTTGTA
GAGTTACTTA TGGTTGTATC CATTGTACTG GAAAGAAACC CCGAGCTAGA ATTTCAAGAC
AAAGTAGATC TAGACAGACT GGTCAAAGAA GCATTTAATG AATTTCAAAA AGATCAGAGT
CGGCTAAAGG AAATTGAAAA ACAAGATGAC ATGACTTCCT TTTACAACAC TCCTCCCCTG
GGAAAAAGAG GAACATGCAG CTATTTGACA AAGGCGGTGA TGAATCTGCT GCTGGAAGGA
GAAGTCAAGC CAAACAATGA TGACCCGTGT CTGATTAGCT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16564
Clone ID Related Accession (Same CDS sequence) NM_001031835 , XM_005255984
Accession Version NM_001031835.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product phosphorylase b kinase regulatory subunit beta isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC007599.6, BC033657.1 and AC092374.4. On Mar 13, 2008 this sequence version replaced gi:73611905. Transcript Variant: This variant (2) has an additional exon in the 5' region and an alternate exon in the 3' region, as compared to variant 1. The resulting isoform (b) is shorter and has an alternate N-terminus and a different segment in the C-terminal region, as compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC033657.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGGCCTGCT CACCTGATGC AGTCGTCTCT CCGTCTTCCG CTTTCTTAAG GTCTGGCTCA 
GTTTATGAAC CTCTTAAAAG CATTAATCTT CCAAGACCTG ATAATGAAAC TCTCTGGGAT
AAGTTGGACC ATTATTACAG AATTGTCAAG TCAACATTGC TGCTGTATCA AAGTCCAACT
ACCGGTCTCT TTCCCACTAA AACATGCGGT GGTGACCAGA AGGCCAAGAT CCAGGACAGC
CTATACTGCG CTGCTGGGGC CTGGGCTTTG GCTCTTGCAT ACAGGCGAAT TGATGATGAC
AAGGGAAGGA CCCATGAGCT GGAGCACTCA GCTATAAAAT GCATGAGAGG AATTCTCTAC
TGCTATATGC GTCAGGCCGA TAAGGTCCAG CAGTTTAAGC AGGATCCACG CCCAACAACA
TGTCTTCACT CTGTTTTCAA TGTGCATACA GGAGATGAGT TGCTTTCCTA TGAGGAATAT
GGTCATCTTC AGATAAATGC AGTGTCACTT TATCTCCTTT ACCTTGTGGA AATGATTTCC
TCAGGACTCC AGATTATCTA CAACACTGAT GAGGTCTCTT TTATTCAAAA CCTTGTATTT
TGTGTGGAAA GAGTTTACCG TGTGCCTGAC TTTGGTGTCT GGGAAAGAGG AAGCAAATAT
AATAATGGCA GCACAGAGCT ACATTCGAGC TCGGTTGGTT TAGCAAAAGC AGCTCTAGAA
GCAATTAATG GATTCAACCT TTTTGGCAAC CAGGGCTGTT CGTGGTCAGT TATATTTGTG
GATCTCGATG CTCACAATCG CAACAGGCAA ACTTTGTGCT CGCTGTTACC CAGAGAATCA
AGATCACATA ATACAGATGC TGCCCTGCTC CCCTGCATCA GTTATCCTGC ATTTGCCCTG
GATGATGAAG TTCTTTTTAG CCAGACACTT GATAAAGTGG TTAGAAAATT AAAAGGAAAA
TATGGATTTA AACGTTTCTT GAGAGATGGG TATAGAACAT CATTGGAAGA TCCCAACAGA
TGCTACTACA AGCCAGCTGA AATTAAGCTA TTTGATGGCA TTGAATGTGA ATTTCCCATA
TTTTTCCTTT ATATGATGAT TGATGGAGTT TTTAGAGGCA ATCCTAAGCA AGTACAGGAA
TATCAGGATC TTTTGACTCC AGTACTTCAT CATACCACAG AAGGATATCC TGTTGTACCA
AAGTACTATT ATGTGCCAGC TGACTTTGTA GAATATGAAA AAAATAACCC TGGTAGTCAA
AAACGATTTC CTAGCAACTG TGGCCGTGAT GGAAAACTGT TTCTTTGGGG ACAAGCACTT
TATATCATCG CAAAACTCCT GGCTGATGAA CTTATTAGTC CTAAAGACAT TGATCCTGTC
CAGCGCTATG TCCCACTAAA GGATCAACGT AACGTGAGCA TGAGGTTTTC CAATCAGGGC
CCACTGGAAA ATGACTTGGT AGTTCATGTG GCACTTATAG CAGAAAGCCA AAGACTTCAA
GTTTTTCTGA ACACATATGG TATTCAAACT CAAACTCCTC AACAAGTAGA ACCCATTCAG
ATATGGCCTC AGCAGGAGCT TGTGAAAGCT TATTTGCAGC TGGGTATCAA TGAAAAGTTA
GGACTCTCTG GAAGGCCAGA CAGGCCCATT GGCTGCCTCG GGACATCAAA GATTTATCGC
ATTCTAGGAA AGACTGTGGT TTGTTACCCG ATTATTTTCG ACCTAAGTGA TTTCTACATG
TCTCAGGATG TTTTCCTGCT GATAGATGAC ATAAAGAATG CGCTGCAGTT CATTAAACAA
TATTGGAAAA TGCATGGACG TCCACTTTTC CTTGTTCTCA TCCGGGAAGA CAATATAAGA
GGTAGCCGGT TCAACCCCAT ATTAGATATG CTGGCAGCCC TTAAAAAAGG AATAATTGGA
GGAGTCAAAG TTCATGTGGA TCGTCTACAG ACACTAATAT CTGGAGCTGT GGTAGAACAA
CTTGATTTCC TACGAATCAG TGACACAGAA GAGCTTCCAG AATTTAAGAG TTTTGAGGAA
CTAGAACCTC CCAAACATTC AAAAGTCAAA CGGCAAAGCA GCACCCCTAG TGCTCCTGAA
CTGGGACAGC AGCCGGATGT CAACATTAGT GAATGGAAGG ACAAACCCAC CCACGAAATT
CTTCAAAAAC TGAATGATTG CAGTTGTCTG GCTAGCCAAG CCATCCTGCT GGGTATACTG
CTCAAAAGAG AAGGCCCCAA CTTCATCACA AAGGAAGGTA CCGTTTCTGA TCACATTGAG
AGAGTCTATA GAAGAGCTGG CAGCCAAAAA CTTTGGTCGG TTGTACGCCG TGCAGCAAGT
CTTTTAAGTA AAGTAGTGGA CAGCCTGGCC CCATCCATTA CTAATGTTTT AGTGCAGGGC
AAACAGGTAA CTCTGGGTGC CTTTGGGCAT GAAGAAGAAG TTATCTCTAA TCCTTTGTCT
CCAAGAGTGA TTCAAAACAT CATCTATTAT AAGTGTAACA CCCATGATGA GAGGGAAGCG
GTCATTCAGC AAGAACTGGT CATCCATATT GGCTGGATCA TCTCCAATAA CCCTGAGTTA
TTCAGTGGCA TGCTGAAAAT ACGAATCGGG TGGATCATCC ATGCCATGGA GTATGAACTT
CAGATCCGTG GCGGAGACAA GCCAGCCTTG GACTTGTATC AGCTGTCACC TAGTGAAGTT
AAACAGCTTC TGCTGGATAT TCTGCAGCCT CAACAGAATG GAAGATGTTG GCTGAACAGG
CGTCAGATCG ATGGGTCTTT GAATAGAACT CCCACCGGGT TCTATGACCG AGTGTGGCAG
ATTCTGGAGC GCACGCCCAA TGGGATCATT GTTGCTGGGA AGCATTTGCC TCAGCAACCA
ACCCTGTCAG ATATGACCAT GTATGAGATG AATTTCTCTC TCCTTGTTGA AGACACGTTG
GGAAATATTG ACCAGCCACA GTACAGACAG ATCGTTGTAG AGTTACTTAT GGTTGTATCC
ATTGTACTGG AAAGAAACCC CGAGCTAGAA TTTCAAGACA AAGTAGATCT AGACAGACTG
GTCAAAGAAG CATTTAATGA ATTTCAAAAA GATCAGAGTC GGCTAAAGGA AATTGAAAAA
CAAGATGACA TGACTTCCTT TTACAACACT CCTCCCCTGG GAAAAAGAGG AACATGCAGC
TATTTGACAA AGGCGGTGAT GAATCTGCTG CTGGAAGGAG AAGTCAAGCC AAACAATGAT
GACCCGTGTC TGATTAGCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001027005.1
CDS186..3446
Misc Feature(1)168..170(+)
Misc Feature(2)309..>1574(+)
Misc Feature(3)2415..2798(+)
Exon (1)1..128
Gene:PHKB
Gene Synonym:
Exon (2)129..240
Gene:PHKB
Gene Synonym:
Exon (3)241..330
Gene:PHKB
Gene Synonym:
Exon (4)331..469
Gene:PHKB
Gene Synonym:
Exon (5)470..569
Gene:PHKB
Gene Synonym:
Exon (6)570..677
Gene:PHKB
Gene Synonym:
Exon (7)678..758
Gene:PHKB
Gene Synonym:
Exon (8)759..874
Gene:PHKB
Gene Synonym:
Exon (9)875..938
Gene:PHKB
Gene Synonym:
Exon (10)939..1034
Gene:PHKB
Gene Synonym:
Exon (11)1035..1232
Gene:PHKB
Gene Synonym:
Exon (12)1233..1290
Gene:PHKB
Gene Synonym:
Exon (13)1291..1368
Gene:PHKB
Gene Synonym:
Exon (14)1369..1527
Gene:PHKB
Gene Synonym:
Exon (15)1528..1622
Gene:PHKB
Gene Synonym:
Exon (16)1623..1678
Gene:PHKB
Gene Synonym:
Exon (17)1679..1772
Gene:PHKB
Gene Synonym:
Exon (18)1773..1856
Gene:PHKB
Gene Synonym:
Exon (19)1857..1961
Gene:PHKB
Gene Synonym:
Exon (20)1962..2044
Gene:PHKB
Gene Synonym:
Exon (21)2045..2135
Gene:PHKB
Gene Synonym:
Exon (22)2136..2197
Gene:PHKB
Gene Synonym:
Exon (23)2198..2360
Gene:PHKB
Gene Synonym:
Exon (24)2361..2442
Gene:PHKB
Gene Synonym:
Exon (25)2443..2500
Gene:PHKB
Gene Synonym:
Exon (26)2501..2591
Gene:PHKB
Gene Synonym:
Exon (27)2592..2794
Gene:PHKB
Gene Synonym:
Exon (28)2795..2929
Gene:PHKB
Gene Synonym:
Exon (29)2930..3059
Gene:PHKB
Gene Synonym:
Exon (30)3060..3167
Gene:PHKB
Gene Synonym:
Exon (31)3168..3308
Gene:PHKB
Gene Synonym:
Exon (32)3309..5603
Gene:PHKB
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001031835
Organism Homo sapiens (human)
Definition Homo sapiens phosphorylase kinase, beta (PHKB), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001031835

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGGCCTGCT CACCTGATGC AGTCGTCTCT CCGTCTTCCG CTTTCTTAAG GTCTGGCTCA 
GTTTATGAAC CTCTTAAAAG CATTAATCTT CCAAGACCTG ATAATGAAAC TCTCTGGGAT
AAGTTGGACC ATTATTACAG AATTGTCAAG TCAACATTGC TGCTGTATCA AAGTCCAACT
ACCGGTCTCT TTCCCACTAA AACATGCGGT GGTGACCAGA AGGCCAAGAT CCAGGACAGC
CTATACTGCG CTGCTGGGGC CTGGGCTTTG GCTCTTGCAT ACAGGCGAAT TGATGATGAC
AAGGGAAGGA CCCATGAGCT GGAGCACTCA GCTATAAAAT GCATGAGAGG AATTCTCTAC
TGCTATATGC GTCAGGCCGA TAAGGTCCAG CAGTTTAAGC AGGATCCACG CCCAACAACA
TGTCTTCACT CTGTTTTCAA TGTGCATACA GGAGATGAGT TGCTTTCCTA TGAGGAATAT
GGTCATCTTC AGATAAATGC AGTGTCACTT TATCTCCTTT ACCTTGTGGA AATGATTTCC
TCAGGACTCC AGATTATCTA CAACACTGAT GAGGTCTCTT TTATTCAAAA CCTTGTATTT
TGTGTGGAAA GAGTTTACCG TGTGCCTGAC TTTGGTGTCT GGGAAAGAGG AAGCAAATAT
AATAATGGCA GCACAGAGCT ACATTCGAGC TCGGTTGGTT TAGCAAAAGC AGCTCTAGAA
GCAATTAATG GATTCAACCT TTTTGGCAAC CAGGGCTGTT CGTGGTCAGT TATATTTGTG
GATCTCGATG CTCACAATCG CAACAGGCAA ACTTTGTGCT CGCTGTTACC CAGAGAATCA
AGATCACATA ATACAGATGC TGCCCTGCTC CCCTGCATCA GTTATCCTGC ATTTGCCCTG
GATGATGAAG TTCTTTTTAG CCAGACACTT GATAAAGTGG TTAGAAAATT AAAAGGAAAA
TATGGATTTA AACGTTTCTT GAGAGATGGG TATAGAACAT CATTGGAAGA TCCCAACAGA
TGCTACTACA AGCCAGCTGA AATTAAGCTA TTTGATGGCA TTGAATGTGA ATTTCCCATA
TTTTTCCTTT ATATGATGAT TGATGGAGTT TTTAGAGGCA ATCCTAAGCA AGTACAGGAA
TATCAGGATC TTTTGACTCC AGTACTTCAT CATACCACAG AAGGATATCC TGTTGTACCA
AAGTACTATT ATGTGCCAGC TGACTTTGTA GAATATGAAA AAAATAACCC TGGTAGTCAA
AAACGATTTC CTAGCAACTG TGGCCGTGAT GGAAAACTGT TTCTTTGGGG ACAAGCACTT
TATATCATCG CAAAACTCCT GGCTGATGAA CTTATTAGTC CTAAAGACAT TGATCCTGTC
CAGCGCTATG TCCCACTAAA GGATCAACGT AACGTGAGCA TGAGGTTTTC CAATCAGGGC
CCACTGGAAA ATGACTTGGT AGTTCATGTG GCACTTATAG CAGAAAGCCA AAGACTTCAA
GTTTTTCTGA ACACATATGG TATTCAAACT CAAACTCCTC AACAAGTAGA ACCCATTCAG
ATATGGCCTC AGCAGGAGCT TGTGAAAGCT TATTTGCAGC TGGGTATCAA TGAAAAGTTA
GGACTCTCTG GAAGGCCAGA CAGGCCCATT GGCTGCCTCG GGACATCAAA GATTTATCGC
ATTCTAGGAA AGACTGTGGT TTGTTACCCG ATTATTTTCG ACCTAAGTGA TTTCTACATG
TCTCAGGATG TTTTCCTGCT GATAGATGAC ATAAAGAATG CGCTGCAGTT CATTAAACAA
TATTGGAAAA TGCATGGACG TCCACTTTTC CTTGTTCTCA TCCGGGAAGA CAATATAAGA
GGTAGCCGGT TCAACCCCAT ATTAGATATG CTGGCAGCCC TTAAAAAAGG AATAATTGGA
GGAGTCAAAG TTCATGTGGA TCGTCTACAG ACACTAATAT CTGGAGCTGT GGTAGAACAA
CTTGATTTCC TACGAATCAG TGACACAGAA GAGCTTCCAG AATTTAAGAG TTTTGAGGAA
CTAGAACCTC CCAAACATTC AAAAGTCAAA CGGCAAAGCA GCACCCCTAG TGCTCCTGAA
CTGGGACAGC AGCCGGATGT CAACATTAGT GAATGGAAGG ACAAACCCAC CCACGAAATT
CTTCAAAAAC TGAATGATTG CAGTTGTCTG GCTAGCCAAG CCATCCTGCT GGGTATACTG
CTCAAAAGAG AAGGCCCCAA CTTCATCACA AAGGAAGGTA CCGTTTCTGA TCACATTGAG
AGAGTCTATA GAAGAGCTGG CAGCCAAAAA CTTTGGTCGG TTGTACGCCG TGCAGCAAGT
CTTTTAAGTA AAGTAGTGGA CAGCCTGGCC CCATCCATTA CTAATGTTTT AGTGCAGGGC
AAACAGGTAA CTCTGGGTGC CTTTGGGCAT GAAGAAGAAG TTATCTCTAA TCCTTTGTCT
CCAAGAGTGA TTCAAAACAT CATCTATTAT AAGTGTAACA CCCATGATGA GAGGGAAGCG
GTCATTCAGC AAGAACTGGT CATCCATATT GGCTGGATCA TCTCCAATAA CCCTGAGTTA
TTCAGTGGCA TGCTGAAAAT ACGAATCGGG TGGATCATCC ATGCCATGGA GTATGAACTT
CAGATCCGTG GCGGAGACAA GCCAGCCTTG GACTTGTATC AGCTGTCACC TAGTGAAGTT
AAACAGCTTC TGCTGGATAT TCTGCAGCCT CAACAGAATG GAAGATGTTG GCTGAACAGG
CGTCAGATCG ATGGGTCTTT GAATAGAACT CCCACCGGGT TCTATGACCG AGTGTGGCAG
ATTCTGGAGC GCACGCCCAA TGGGATCATT GTTGCTGGGA AGCATTTGCC TCAGCAACCA
ACCCTGTCAG ATATGACCAT GTATGAGATG AATTTCTCTC TCCTTGTTGA AGACACGTTG
GGAAATATTG ACCAGCCACA GTACAGACAG ATCGTTGTAG AGTTACTTAT GGTTGTATCC
ATTGTACTGG AAAGAAACCC CGAGCTAGAA TTTCAAGACA AAGTAGATCT AGACAGACTG
GTCAAAGAAG CATTTAATGA ATTTCAAAAA GATCAGAGTC GGCTAAAGGA AATTGAAAAA
CAAGATGACA TGACTTCCTT TTACAACACT CCTCCCCTGG GAAAAAGAGG AACATGCAGC
TATTTGACAA AGGCGGTGAT GAATCTGCTG CTGGAAGGAG AAGTCAAGCC AAACAATGAT
GACCCGTGTC TGATTAGCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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