This gene encodes a member of the serpin family of proteins, a group of proteins that inhibit serine proteases. Thrombin, urokinase, plasmin and trypsin are among the proteases that this family member can inhibit. This gene is a susceptibility gene for chronic obstructive pulmonary disease and for emphysema. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012].
The following SERPINE2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SERPINE2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC366668.1, AK225823.1, AC073641.3, AI754402.1 and DB325994.1.
Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in an isoform (b) that is 1 aa shorter than isoform a.
Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: SRR1803614.240949.1, SRR1660805.157452.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMEA2142853, SAMEA2145245 [ECO:0000348]
##Evidence-Data-END##
##RefSeq-Attributes-START##
RefSeq Select criteria :: based on conservation, expression
##RefSeq-Attributes-END##
COMPLETENESS: complete on the 3' end.
Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC366668.1, BC015663.2, BX360097.2, AI754402.1 and DB325994.1. This sequence is a reference standard in the RefSeqGene project. On Nov 4, 2008 this sequence version replaced NM_006216.2.
Transcript Variant: This variant (1) encodes isoform a.
Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: SRR1803613.12678.1, SRR1660807.52910.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMEA2145245, SAMEA2145743 [ECO:0000348]
##Evidence-Data-END##
COMPLETENESS: complete on the 3' end.
Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process
##Genome-Annotation-Data-START##
Annotation Provider :: NCBI
Annotation Status :: Full annotation
Annotation Version :: Homo sapiens Annotation Release 108
Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline
Annotation Software Version :: 7.0
Annotation Method :: Best-placed RefSeq; Gnomon
Features Annotated :: Gene; mRNA; CDS; ncRNA
##Genome-Annotation-Data-END##
Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process
##Genome-Annotation-Data-START##
Annotation Provider :: NCBI
Annotation Status :: Full annotation
Annotation Version :: Homo sapiens Annotation Release 108
Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline
Annotation Software Version :: 7.0
Annotation Method :: Best-placed RefSeq; Gnomon
Features Annotated :: Gene; mRNA; CDS; ncRNA
##Genome-Annotation-Data-END##
Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process
On Mar 26, 2018 this sequence version replaced XM_017004332.1.
##Genome-Annotation-Data-START##
Annotation Provider :: NCBI
Annotation Status :: Updated annotation
Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607
Annotation Version :: 109.20190607
Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline
Annotation Software Version :: 8.2
Annotation Method :: Best-placed RefSeq; propagated RefSeq model
Features Annotated :: Gene; mRNA; CDS; ncRNA
Annotation Freeze :: 20190607
##Genome-Annotation-Data-END##
Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process
##Genome-Annotation-Data-START##
Annotation Provider :: NCBI
Annotation Status :: Full annotation
Annotation Version :: Homo sapiens Annotation Release 108
Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline
Annotation Software Version :: 7.0
Annotation Method :: Best-placed RefSeq; Gnomon
Features Annotated :: Gene; mRNA; CDS; ncRNA
##Genome-Annotation-Data-END##
Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process
##Genome-Annotation-Data-START##
Annotation Provider :: NCBI
Annotation Status :: Updated annotation
Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607
Annotation Version :: 109.20190607
Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline
Annotation Software Version :: 8.2
Annotation Method :: Best-placed RefSeq; propagated RefSeq model
Features Annotated :: Gene; mRNA; CDS; ncRNA
Annotation Freeze :: 20190607
##Genome-Annotation-Data-END##
Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC304261.1, AK295564.1, BX360097.2, AI754402.1 and DB325994.1.
Transcript Variant: This variant (4) differs in the 5' UTR and 5' coding region, uses an alternate start codon, and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The resulting isoform (c) has a distinct N-terminus and is longer than isoform a.
CCDS Note: This CCDS representation is supported by the mRNA AK295564.1. It contains an alternative 5' exon compared to other variants at this locus, as represented by CCDS2460.1 and CCDS46526.1. It should be noted that the annotated start codon in this CCDS has a weak Kozak signal and is only conserved among primate species. It is possible that leaky scanning by ribosomes could occur to allow a downstream start codon to be used at least some of the time. The downstream start codon has a stronger Kozak signal and is much better conserved, and it would result in a protein that is 12 aa shorter at the N-terminus. There is no experimental evidence indicating which start codon is preferentially used by this variant in vivo. Signal peptides can be predicted for both the longer and shorter N-termini (cleavage following aa 31 or aa 19, respectively), but it should be noted that the probability of signal peptide occurrence is reduced for the longer N-terminus, as predicted by SignalP 3.0 and Phobius. It is unknown if a signal peptide is cleaved from the longer protein in vivo.
Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: SRR1660809.123330.1, SRR1660803.201569.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMEA2142853, SAMEA2145245 [ECO:0000348]
##Evidence-Data-END##
COMPLETENESS: complete on the 3' end.
Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process
On Mar 12, 2015 this sequence version replaced XM_005246641.1.
##Genome-Annotation-Data-START##
Annotation Provider :: NCBI
Annotation Status :: Updated annotation
Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607
Annotation Version :: 109.20190607
Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline
Annotation Software Version :: 8.2
Annotation Method :: Best-placed RefSeq; propagated RefSeq model
Features Annotated :: Gene; mRNA; CDS; ncRNA
Annotation Freeze :: 20190607
##Genome-Annotation-Data-END##
User Manual
NM_001136528.1
