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PLCB4 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol PLCB4
Entrez Gene ID 5332
Full Name phospholipase C beta 4
Synonyms ARCND2,PI-PLC
General protein information
Preferred Names

phospholipase C beta 4

Names

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4
1-phosphatidyl-D-myo-inositol-4,5-bisphosphate
PLC-beta-4
dJ1119D9.2 (Phopholipase C, beta 4 (1-Phosphatidylinositol-4,5-Bisphosphate Phosphodiesterase Beta 4))
inositoltrisphosphohydrolase
monophosphatidylinositol phosphodiesterase
phosphoinositidase C
phosphoinositide phospholipase C-beta-4
triphosphoinositide phosphodiesterase

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

20

20p12.3-p12.2

Summary The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals in the retina. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2010].
Disorder MIM:

600810


mRNA and Protein(s)

mRNA Protein Name
NM_001172646.1 NP_001166117.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform c
XM_006723569.2 XP_006723632.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
XM_017027882.1 XP_016883371.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X4
XM_005260724.2 XP_005260781.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
XM_017027881.1 XP_016883370.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X3
XM_017027883.1 XP_016883372.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X5
NM_000933.3 NP_000924.3 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform a
XM_005260728.2 XP_005260785.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X8
XM_017027886.1 XP_016883375.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X9
NM_182797.2 NP_877949.2 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform b
XM_017027880.1 XP_016883369.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
XM_017027885.1 XP_016883374.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X6
XM_006723568.3 XP_006723631.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
XM_017027884.1 XP_016883373.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X6
XM_011529254.2 XP_011527556.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X7



Macaca mulatta (rhesus monkey) PLCB4 XP_002798260.1
Canis lupus familiaris (dog) PLCB4 XP_859719.2
Bos taurus (cattle) PLCB4 NP_001159982.1
Mus musculus (house mouse) Plcb4 NP_038857.1
Rattus norvegicus (Norway rat) Plcb4 NP_077329.1
Gallus gallus (chicken) PLCB4 NP_001186364.1
Danio rerio (zebrafish) LOC100537538 XP_003200348.2
Homo sapiens (human) PLCB4 NP_877949.2
Anopheles gambiae (African malaria mosquito) AgaP_AGAP001936 XP_003435972.1
Drosophila melanogaster (fruit fly) norpA NP_001014720.1
Xenopus tropicalis (tropical clawed frog) plcb4 XP_004914780.1
Pan troglodytes (chimpanzee) PLCB4 XP_001168348.2
Caenorhabditis elegans (roundworm) egl-8 NP_001021346.1


Related articles in PubMed

Deep sequencing of uveal melanoma identifies a recurrent mutation in PLCB4.
Johansson P, Aoude LG, Wadt K, Glasson WJ, Warrier SK, Hewitt AW, Kiilgaard JF, Heegaard S, Isaacs T, Franchina M, Ingvar C, Vermeulen T, Whitehead KJ, Schmidt CW, Palmer JM, Symmons J, Gerdes AM, Jönsson G, Hayward NK
Oncotarget7(4)4624-31(2016 Jan)

Genetic variants in PLCB4/PLCB1 as susceptibility loci for coronary artery aneurysm formation in Kawasaki disease in Han Chinese in Taiwan.
Lin YJ, Chang JS, Liu X, Tsang H, Chien WK, Chen JH, Hsieh HY, Hsueh KC, Shiao YT, Li JP, Lin CW, Lai CH, Wu JY, Chen CH, Lin JG, Lin TH, Liao CC, Huang SM, Lan YC, Ho TJ, Liang WM, Yeh YC, Lin JC, Tsai FJ
Scientific reports514762(2015 Oct)

Gene expression profile in fibroblasts of Huntington's disease patients and controls.
Marchina E, Misasi S, Bozzato A, Ferraboli S, Agosti C, Rozzini L, Borsani G, Barlati S, Padovani A
Journal of the neurological sciences337(1-2)42-6(2014 Feb)

Phospholipase C-β1 and β4 contribute to non-genetic cell-to-cell variability in histamine-induced calcium signals in HeLa cells.
Ishida S, Matsu-Ura T, Fukami K, Michikawa T, Mikoshiba K
PloS one9(1)e86410(2014)

Further characterization of atypical features in auriculocondylar syndrome caused by recessive PLCB4 mutations.
Kido Y, Gordon CT, Sakazume S, Ben Bdira E, Dattani M, Wilson LC, Lyonnet S, Murakami N, Cunningham ML, Amiel J, Nagai T
American journal of medical genetics. Part A161A(9)2339-46(2013 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

recurrent mutation in PLCB4 is associated with uveal melanoma.
Title: Deep sequencing of uveal melanoma identifies a recurrent mutation in PLCB4.

Polymorphism of the PLCB4/B1 genes might be involved in the coronary artery aneurysm pathogenesis of Kawasaki disease.
Title: Genetic variants in PLCB4/PLCB1 as susceptibility loci for coronary artery aneurysm formation in Kawasaki disease in Han Chinese in Taiwan.

This study demonistrated by Gene expression profile that PLCB4 upregulaion in fibroblasts of Huntington's disease patients.
Title: Gene expression profile in fibroblasts of Huntington's disease patients and controls.

Phospholipase C-beta1 and beta4 contribute to non-genetic cell-to-cell variability in histamine-induced calcium signals in HeLa cells.
Title: Phospholipase C-β1 and β4 contribute to non-genetic cell-to-cell variability in histamine-induced calcium signals in HeLa cells.

Auriculocondylar syndrome is caused by PLCB4 mutations inherited not only in an autosomal dominant manner (catalytic domain missense mutations) but also inherited as autosomal recessive (complete loss of function).
Title: Further characterization of atypical features in auriculocondylar syndrome caused by recessive PLCB4 mutations.

The following PLCB4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLCB4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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Up to 50% OFF! Starting
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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu52720 XM_005260728.2
Latest version!
Homo sapiens phospholipase C beta 4 (PLCB4), transcript variant X11, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu93038 XM_017027886.1
Latest version!
Homo sapiens phospholipase C beta 4 (PLCB4), transcript variant X12, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $475.30
$679.00
OHu93035 XM_017027882.1
Latest version!
Homo sapiens phospholipase C beta 4 (PLCB4), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $559.30
$799.00
OHu93036 XM_017027883.1
Latest version!
Homo sapiens phospholipase C beta 4 (PLCB4), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $559.30
$799.00
OHu93037 XM_017027884.1
Latest version!
Homo sapiens phospholipase C beta 4 (PLCB4), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $559.30
$799.00
OHu93037 XM_017027885.1
Latest version!
Homo sapiens phospholipase C beta 4 (PLCB4), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $559.30
$799.00
OHu14145 NM_000933.3
Latest version!
Homo sapiens phospholipase C beta 4 (PLCB4), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu14162 NM_182797.2
Latest version!
Homo sapiens phospholipase C beta 4 (PLCB4), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu18159 NM_001172646.1
Latest version!
Homo sapiens phospholipase C beta 4 (PLCB4), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $839.30
$1199.00
OHu14145 XM_017027880.1
Latest version!
Homo sapiens phospholipase C beta 4 (PLCB4), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu14162 XM_017027881.1
Latest version!
Homo sapiens phospholipase C beta 4 (PLCB4), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu52719 XM_006723568.3
Latest version!
Homo sapiens phospholipase C beta 4 (PLCB4), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu52719 XM_006723569.2
Latest version!
Homo sapiens phospholipase C beta 4 (PLCB4), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu52719 XM_005260724.2
Latest version!
Homo sapiens phospholipase C beta 4 (PLCB4), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu59763 XM_011529254.2
Latest version!
Homo sapiens phospholipase C beta 4 (PLCB4), transcript variant X10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu52720
Clone ID Related Accession (Same CDS sequence) XM_005260728.2
Accession Version XM_005260728.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2817bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X8
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005260728.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
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961
1021
1081
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1381
1441
1501
1561
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1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
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2521
2581
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2701
2761
ATGCAGATCA TTGAGATGTA TGAACCTGAT GAAGATTTGA AGAAAAAAGG CCTTATATCA 
AGTGATGGGT TTTGCAGATA TCTGATGTCA GATGAAAACG CCCCAGTCTT CCTAGATCGT
TTAGAACTTT ACCAAGAAAT GGACCATCCT CTGGCTCACT ACTTCATCAG TTCTTCCCAT
AACACTTATC TCACTGGCAG ACAGTTCGGC GGGAAGTCTT CGGTAGAAAT GTACAGACAG
GTTCTCCTGG CTGGTTGCAG ATGTGTTGAA CTTGACTGCT GGGATGGAAA AGGTGAAGAC
CAAGAACCAA TAATAACTCA TGGAAAAGCA ATGTGTACAG ATATCCTTTT TAAGGATGTA
ATTCAAGCCA TCAAGGAAAC TGCATTTGTC ACATCAGAAT ATCCTGTAAT TCTCTCCTTT
GAAAATCACT GCAGCAAATA TCAACAGTAC AAGATGTCCA AATATTGCGA AGATCTATTT
GGGGATCTCC TGTTGAAACA AGCACTTGAA TCACATCCAC TTGAACCAGG CAGGGCTTTG
CCATCCCCCA ATGACCTCAA AAGAAAAATA CTCATAAAAA ACAAGCGGCT GAAACCTGAA
GTTGAAAAAA AACAGCTGGA AGCTTTGAGA AGCATGATGG AAGCTGGAGA ATCTGCCTCC
CCAGCAAACA TCTTAGAGGA CGATAATGAA GAGGAGATCG AAAGTGCTGA CCAAGAGGAG
GAAGCTCACC CCGAATTCAA ATTTGGAAAT GAACTTTCTG CTGATGACTT GGGTCACAAG
GAAGCTGTTG CAAATAGCGT CAAGAAGGCT TCAGATGACC TTGAACATGA AAACAACAAA
AAGGGCCTGG TCACTGTAGA AGATGAGCAG GCGTGGATGG CATCTTATAA ATATGTAGGT
GCTACCACTA ATATCCATCC ATATTTGTCC ACAATGATCA ACTACGCCCA GCCTGTAAAG
TTTCAAGGTT TCCATGTGGC AGAAGAACGC AATATTCATT ATAACATGTC TTCTTTTAAT
GAATCAGTCG GTCTTGGCTA CTTGAAGACA CATGCAATTG AATTTGTCAA TTATAACAAA
CGGCAAATGA GTCGCATTTA CCCCAAGGGA GGCCGAGTCG ATTCCAGTAA TTACATGCCT
CAGATTTTCT GGAACGCTGG CTGCCAGATG GTTTCACTGA ACTATCAAAC CCCAGATTTA
GCGATGCAAT TGAATCAGGG AAAATTTGAG TATAATGGAT CGTGCGGGTA CCTTCTCAAA
CCAGATTTCA TGAGGCGGCC TGATCGAACA TTTGACCCCT TCTCTGAAAC TCCTGTTGAT
GGTGTTATTG CAGCCACTTG CTCAGTGCAG GTTATATCAG GTCAATTCTT ATCAGATAAG
AAAATTGGCA CCTACGTAGA GGTGGATATG TATGGGTTGC CCACTGACAC CATACGTAAG
GAATTCCGAA CTCGCATGGT TATGAATAAT GGACTCAATC CAGTTTACAA TGAAGAGTCA
TTTGTATTTC GGAAGGTGAT CCTGCCGGAC CTGGCTGTCT TGAGAATAGC TGTGTATGAT
GATAACAACA AGCTGATTGG CCAGAGGATC CTCCCGCTTG ATGGCCTCCA AGCCGGATAT
CGACACATTT CCCTTCGAAA TGAGGGAAAT AAACCATTAT CACTACCAAC AATTTTCTGC
AATATTGTTC TTAAAACATA TGTGCCTGAT GGATTTGGAG ATATCGTGGA TGCTTTATCA
GATCCAAAGA AATTTCTCTC AATTACAGAA AAGAGAGCAG ACCAAATGAG AGCTATGGGC
ATTGAAACTA GTGACATAGC CGACGTGCCC AGTGACACTT CCAAAAATGA CAAGAAAGGA
AAGGCCAACA CCGCCAAAGC AAATGTGACC CCTCAGAGTA GCTCTGAGCT CAGACCAACC
ACCACGGCTG CCCTGGCCTC TGGTGTGGAA GCCAAGAAAG GTATTGAACT TATCCCTCAA
GTAAGGATAG AAGACTTAAA GCAGATGAAG GCTTACTTGA AGCATTTAAA GAAACAGCAG
AAGGAGCTAA ATTCTTTAAA GAAGAAACAT GCAAAGGAAC ACAGTACCAT GCAGAAGTTA
CACTGCACGC AAGTTGACAA AATTGTGGCA CAGTATGACA AAGAGAAGTC GACTCATGAG
AAAATCCTAG AGAAGGCAAT GAAGAAGAAG GGGGGAAGTA ATTGTCTCGA AATGAAAAAA
GAAACAGAAA TCAAAATTCA GACGCTGACA TCAGATCACA AATCTAAGGT CAAAGAGATT
GTAGCACAGC ACACAAAGGA ATGGTCAGAA ATGATCAATA CCCACAGTGC TGAGGAGCAA
GAAATCCGAG ACCTGCACCT CAGCCAGCAG TGTGAGCTGC TGAAAAAGCT ACTCATCAAT
GCCCACGAGC AGCAAACCCA GCAGCTGAAA CTGTCCCATG ACAGGGAAAG CAAGGAAATG
CGAGCACACC AGGCTAAGAT TTCTATGGAA AATAGCAAAG CCATCAGCCA AGATAAATCT
ATCAAGAATA AAGCAGAACG GGAAAGGCGA GTCAGGGAGT TAAACAGCAG CAACACTAAA
AAGTTTCTGG AAGAAAGAAA GAGACTTGCC ATGAAGCAGT CCAAAGAAAT GGATCAGTTG
AAAAAAGTCC AGCTTGAACA TCTAGAATTC CTAGAGAAAC AGAATGAGCA GCTTTTGAAA
TCCTGTCATG CAGTGTCCCA AACGCAAGGC GAAGGAGATG CAGCAGATGG TGAAATTGGA
AGCCGAGATG GACCGCAGAC CAGCAACAGT AGTATGAAAC TCCAAAATGC AAACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005260785.1
CDS244..3060
Misc Feature(1)<244..375
Misc Feature(2)373..1479
Misc Feature(3)order(421..426,511..513,517..519,562..564,664..666,820..822,826..828,1255..1257,1336..1338,1342..1344)
Misc Feature(4)order(421..423,562..564)
Misc Feature(5)order(424..426,511..513,517..519,664..666)
Misc Feature(6)1579..1929
Misc Feature(7)order(1630..1632,1708..1710,1801..1803,1807..1809,1813..1815,1819..1821)
Misc Feature(8)2215..2337
Misc Feature(9)2260..2973
Translation

Target ORF information:

RefSeq Version XM_005260728.2
Organism Homo sapiens(Human)
Definition Homo sapiens phospholipase C beta 4 (PLCB4), transcript variant X11, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005260728.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
ATGCAGATCA TTGAGATGTA TGAACCTGAT GAAGATTTGA AGAAAAAAGG CCTTATATCA 
AGTGATGGGT TTTGCAGATA TCTGATGTCA GATGAAAACG CCCCAGTCTT CCTAGATCGT
TTAGAACTTT ACCAAGAAAT GGACCATCCT CTGGCTCACT ACTTCATCAG TTCTTCCCAT
AACACTTATC TCACTGGCAG ACAGTTCGGC GGGAAGTCTT CGGTAGAAAT GTACAGACAG
GTTCTCCTGG CTGGTTGCAG ATGTGTTGAA CTTGACTGCT GGGATGGAAA AGGTGAAGAC
CAAGAACCAA TAATAACTCA TGGAAAAGCA ATGTGTACAG ATATCCTTTT TAAGGATGTA
ATTCAAGCCA TCAAGGAAAC TGCATTTGTC ACATCAGAAT ATCCTGTAAT TCTCTCCTTT
GAAAATCACT GCAGCAAATA TCAACAGTAC AAGATGTCCA AATATTGCGA AGATCTATTT
GGGGATCTCC TGTTGAAACA AGCACTTGAA TCACATCCAC TTGAACCAGG CAGGGCTTTG
CCATCCCCCA ATGACCTCAA AAGAAAAATA CTCATAAAAA ACAAGCGGCT GAAACCTGAA
GTTGAAAAAA AACAGCTGGA AGCTTTGAGA AGCATGATGG AAGCTGGAGA ATCTGCCTCC
CCAGCAAACA TCTTAGAGGA CGATAATGAA GAGGAGATCG AAAGTGCTGA CCAAGAGGAG
GAAGCTCACC CCGAATTCAA ATTTGGAAAT GAACTTTCTG CTGATGACTT GGGTCACAAG
GAAGCTGTTG CAAATAGCGT CAAGAAGGCT TCAGATGACC TTGAACATGA AAACAACAAA
AAGGGCCTGG TCACTGTAGA AGATGAGCAG GCGTGGATGG CATCTTATAA ATATGTAGGT
GCTACCACTA ATATCCATCC ATATTTGTCC ACAATGATCA ACTACGCCCA GCCTGTAAAG
TTTCAAGGTT TCCATGTGGC AGAAGAACGC AATATTCATT ATAACATGTC TTCTTTTAAT
GAATCAGTCG GTCTTGGCTA CTTGAAGACA CATGCAATTG AATTTGTCAA TTATAACAAA
CGGCAAATGA GTCGCATTTA CCCCAAGGGA GGCCGAGTCG ATTCCAGTAA TTACATGCCT
CAGATTTTCT GGAACGCTGG CTGCCAGATG GTTTCACTGA ACTATCAAAC CCCAGATTTA
GCGATGCAAT TGAATCAGGG AAAATTTGAG TATAATGGAT CGTGCGGGTA CCTTCTCAAA
CCAGATTTCA TGAGGCGGCC TGATCGAACA TTTGACCCCT TCTCTGAAAC TCCTGTTGAT
GGTGTTATTG CAGCCACTTG CTCAGTGCAG GTTATATCAG GTCAATTCTT ATCAGATAAG
AAAATTGGCA CCTACGTAGA GGTGGATATG TATGGGTTGC CCACTGACAC CATACGTAAG
GAATTCCGAA CTCGCATGGT TATGAATAAT GGACTCAATC CAGTTTACAA TGAAGAGTCA
TTTGTATTTC GGAAGGTGAT CCTGCCGGAC CTGGCTGTCT TGAGAATAGC TGTGTATGAT
GATAACAACA AGCTGATTGG CCAGAGGATC CTCCCGCTTG ATGGCCTCCA AGCCGGATAT
CGACACATTT CCCTTCGAAA TGAGGGAAAT AAACCATTAT CACTACCAAC AATTTTCTGC
AATATTGTTC TTAAAACATA TGTGCCTGAT GGATTTGGAG ATATCGTGGA TGCTTTATCA
GATCCAAAGA AATTTCTCTC AATTACAGAA AAGAGAGCAG ACCAAATGAG AGCTATGGGC
ATTGAAACTA GTGACATAGC CGACGTGCCC AGTGACACTT CCAAAAATGA CAAGAAAGGA
AAGGCCAACA CCGCCAAAGC AAATGTGACC CCTCAGAGTA GCTCTGAGCT CAGACCAACC
ACCACGGCTG CCCTGGCCTC TGGTGTGGAA GCCAAGAAAG GTATTGAACT TATCCCTCAA
GTAAGGATAG AAGACTTAAA GCAGATGAAG GCTTACTTGA AGCATTTAAA GAAACAGCAG
AAGGAGCTAA ATTCTTTAAA GAAGAAACAT GCAAAGGAAC ACAGTACCAT GCAGAAGTTA
CACTGCACGC AAGTTGACAA AATTGTGGCA CAGTATGACA AAGAGAAGTC GACTCATGAG
AAAATCCTAG AGAAGGCAAT GAAGAAGAAG GGGGGAAGTA ATTGTCTCGA AATGAAAAAA
GAAACAGAAA TCAAAATTCA GACGCTGACA TCAGATCACA AATCTAAGGT CAAAGAGATT
GTAGCACAGC ACACAAAGGA ATGGTCAGAA ATGATCAATA CCCACAGTGC TGAGGAGCAA
GAAATCCGAG ACCTGCACCT CAGCCAGCAG TGTGAGCTGC TGAAAAAGCT ACTCATCAAT
GCCCACGAGC AGCAAACCCA GCAGCTGAAA CTGTCCCATG ACAGGGAAAG CAAGGAAATG
CGAGCACACC AGGCTAAGAT TTCTATGGAA AATAGCAAAG CCATCAGCCA AGATAAATCT
ATCAAGAATA AAGCAGAACG GGAAAGGCGA GTCAGGGAGT TAAACAGCAG CAACACTAAA
AAGTTTCTGG AAGAAAGAAA GAGACTTGCC ATGAAGCAGT CCAAAGAAAT GGATCAGTTG
AAAAAAGTCC AGCTTGAACA TCTAGAATTC CTAGAGAAAC AGAATGAGCA GCTTTTGAAA
TCCTGTCATG CAGTGTCCCA AACGCAAGGC GAAGGAGATG CAGCAGATGG TGAAATTGGA
AGCCGAGATG GACCGCAGAC CAGCAACAGT AGTATGAAAC TCCAAAATGC AAACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu93038
Clone ID Related Accession (Same CDS sequence) XM_017027886.1
Accession Version XM_017027886.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2781bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X9
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
ATGCAGATCA TTGAGATGTA TGAACCTGAT GAAGATTTGA AGAAAAAAGG CCTTATATCA 
AGTGATGGGT TTTGCAGATA TCTGATGTCA GATGAAAACG CCCCAGTCTT CCTAGATCGT
TTAGAACTTT ACCAAGAAAT GGACCATCCT CTGGCTCACT ACTTCATCAG TTCTTCCCAT
AACACTTATC TCACTGGCAG ACAGTTCGGC GGGAAGTCTT CGGTAGAAAT GTACAGACAG
GTTCTCCTGG CTGGTTGCAG ATGTGTTGAA CTTGACTGCT GGGATGGAAA AGGTGAAGAC
CAAGAACCAA TAATAACTCA TGGAAAAGCA ATGTGTACAG ATATCCTTTT TAAGGATGTA
ATTCAAGCCA TCAAGGAAAC TGCATTTGTC ACATCAGAAT ATCCTGTAAT TCTCTCCTTT
GAAAATCACT GCAGCAAATA TCAACAGTAC AAGATGTCCA AATATTGCGA AGATCTATTT
GGGGATCTCC TGTTGAAACA AGCACTTGAA TCACATCCAC TTGAACCAGG CAGGGCTTTG
CCATCCCCCA ATGACCTCAA AAGAAAAATA CTCATAAAAA ACAAGCGGCT GAAACCTGAA
GTTGAAAAAA AACAGCTGGA AGCTTTGAGA AGCATGATGG AAGCTGGAGA ATCTGCCTCC
CCAGCAAACA TCTTAGAGGA CGATAATGAA GAGGAGATCG AAAGTGCTGA CCAAGAGGAG
GAAGCTCACC CCGAATTCAA ATTTGGAAAT GAACTTTCTG CTGATGACTT GGGTCACAAG
GAAGCTGTTG CAAATAGCGT CAAGAAGGGC CTGGTCACTG TAGAAGATGA GCAGGCGTGG
ATGGCATCTT ATAAATATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG
ATCAACTACG CCCAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCAGAAGA ACGCAATATT
CATTATAACA TGTCTTCTTT TAATGAATCA GTCGGTCTTG GCTACTTGAA GACACATGCA
ATTGAATTTG TCAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGA
GTCGATTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCA
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
GGATCGTGCG GGTACCTTCT CAAACCAGAT TTCATGAGGC GGCCTGATCG AACATTTGAC
CCCTTCTCTG AAACTCCTGT TGATGGTGTT ATTGCAGCCA CTTGCTCAGT GCAGGTTATA
TCAGGTCAAT TCTTATCAGA TAAGAAAATT GGCACCTACG TAGAGGTGGA TATGTATGGG
TTGCCCACTG ACACCATACG TAAGGAATTC CGAACTCGCA TGGTTATGAA TAATGGACTC
AATCCAGTTT ACAATGAAGA GTCATTTGTA TTTCGGAAGG TGATCCTGCC GGACCTGGCT
GTCTTGAGAA TAGCTGTGTA TGATGATAAC AACAAGCTGA TTGGCCAGAG GATCCTCCCG
CTTGATGGCC TCCAAGCCGG ATATCGACAC ATTTCCCTTC GAAATGAGGG AAATAAACCA
TTATCACTAC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
GGAGATATCG TGGATGCTTT ATCAGATCCA AAGAAATTTC TCTCAATTAC AGAAAAGAGA
GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCCGACGT GCCCAGTGAC
ACTTCCAAAA ATGACAAGAA AGGAAAGGCC AACACCGCCA AAGCAAATGT GACCCCTCAG
AGTAGCTCTG AGCTCAGACC AACCACCACG GCTGCCCTGG CCTCTGGTGT GGAAGCCAAG
AAAGGTATTG AACTTATCCC TCAAGTAAGG ATAGAAGACT TAAAGCAGAT GAAGGCTTAC
TTGAAGCATT TAAAGAAACA GCAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCAAAG
GAACACAGTA CCATGCAGAA GTTACACTGC ACGCAAGTTG ACAAAATTGT GGCACAGTAT
GACAAAGAGA AGTCGACTCA TGAGAAAATC CTAGAGAAGG CAATGAAGAA GAAGGGGGGA
AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATCAAAA TTCAGACGCT GACATCAGAT
CACAAATCTA AGGTCAAAGA GATTGTAGCA CAGCACACAA AGGAATGGTC AGAAATGATC
AATACCCACA GTGCTGAGGA GCAAGAAATC CGAGACCTGC ACCTCAGCCA GCAGTGTGAG
CTGCTGAAAA AGCTACTCAT CAATGCCCAC GAGCAGCAAA CCCAGCAGCT GAAACTGTCC
CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAATAGC
AAAGCCATCA GCCAAGATAA ATCTATCAAG AATAAAGCAG AACGGGAAAG GCGAGTCAGG
GAGTTAAACA GCAGCAACAC TAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCCATGAAG
CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACATCTAGA ATTCCTAGAG
AAACAGAATG AGCAGCTTTT GAAATCCTGT CATGCAGTGT CCCAAACGCA AGGCGAAGGA
GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTATG
AAACTCCAAA ATGCAAACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016883375.1
CDS245..3025
Translation

Target ORF information:

RefSeq Version XM_017027886.1
Organism Homo sapiens(Human)
Definition Homo sapiens phospholipase C beta 4 (PLCB4), transcript variant X12, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017027886.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
ATGCAGATCA TTGAGATGTA TGAACCTGAT GAAGATTTGA AGAAAAAAGG CCTTATATCA 
AGTGATGGGT TTTGCAGATA TCTGATGTCA GATGAAAACG CCCCAGTCTT CCTAGATCGT
TTAGAACTTT ACCAAGAAAT GGACCATCCT CTGGCTCACT ACTTCATCAG TTCTTCCCAT
AACACTTATC TCACTGGCAG ACAGTTCGGC GGGAAGTCTT CGGTAGAAAT GTACAGACAG
GTTCTCCTGG CTGGTTGCAG ATGTGTTGAA CTTGACTGCT GGGATGGAAA AGGTGAAGAC
CAAGAACCAA TAATAACTCA TGGAAAAGCA ATGTGTACAG ATATCCTTTT TAAGGATGTA
ATTCAAGCCA TCAAGGAAAC TGCATTTGTC ACATCAGAAT ATCCTGTAAT TCTCTCCTTT
GAAAATCACT GCAGCAAATA TCAACAGTAC AAGATGTCCA AATATTGCGA AGATCTATTT
GGGGATCTCC TGTTGAAACA AGCACTTGAA TCACATCCAC TTGAACCAGG CAGGGCTTTG
CCATCCCCCA ATGACCTCAA AAGAAAAATA CTCATAAAAA ACAAGCGGCT GAAACCTGAA
GTTGAAAAAA AACAGCTGGA AGCTTTGAGA AGCATGATGG AAGCTGGAGA ATCTGCCTCC
CCAGCAAACA TCTTAGAGGA CGATAATGAA GAGGAGATCG AAAGTGCTGA CCAAGAGGAG
GAAGCTCACC CCGAATTCAA ATTTGGAAAT GAACTTTCTG CTGATGACTT GGGTCACAAG
GAAGCTGTTG CAAATAGCGT CAAGAAGGGC CTGGTCACTG TAGAAGATGA GCAGGCGTGG
ATGGCATCTT ATAAATATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG
ATCAACTACG CCCAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCAGAAGA ACGCAATATT
CATTATAACA TGTCTTCTTT TAATGAATCA GTCGGTCTTG GCTACTTGAA GACACATGCA
ATTGAATTTG TCAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGA
GTCGATTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCA
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
GGATCGTGCG GGTACCTTCT CAAACCAGAT TTCATGAGGC GGCCTGATCG AACATTTGAC
CCCTTCTCTG AAACTCCTGT TGATGGTGTT ATTGCAGCCA CTTGCTCAGT GCAGGTTATA
TCAGGTCAAT TCTTATCAGA TAAGAAAATT GGCACCTACG TAGAGGTGGA TATGTATGGG
TTGCCCACTG ACACCATACG TAAGGAATTC CGAACTCGCA TGGTTATGAA TAATGGACTC
AATCCAGTTT ACAATGAAGA GTCATTTGTA TTTCGGAAGG TGATCCTGCC GGACCTGGCT
GTCTTGAGAA TAGCTGTGTA TGATGATAAC AACAAGCTGA TTGGCCAGAG GATCCTCCCG
CTTGATGGCC TCCAAGCCGG ATATCGACAC ATTTCCCTTC GAAATGAGGG AAATAAACCA
TTATCACTAC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
GGAGATATCG TGGATGCTTT ATCAGATCCA AAGAAATTTC TCTCAATTAC AGAAAAGAGA
GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCCGACGT GCCCAGTGAC
ACTTCCAAAA ATGACAAGAA AGGAAAGGCC AACACCGCCA AAGCAAATGT GACCCCTCAG
AGTAGCTCTG AGCTCAGACC AACCACCACG GCTGCCCTGG CCTCTGGTGT GGAAGCCAAG
AAAGGTATTG AACTTATCCC TCAAGTAAGG ATAGAAGACT TAAAGCAGAT GAAGGCTTAC
TTGAAGCATT TAAAGAAACA GCAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCAAAG
GAACACAGTA CCATGCAGAA GTTACACTGC ACGCAAGTTG ACAAAATTGT GGCACAGTAT
GACAAAGAGA AGTCGACTCA TGAGAAAATC CTAGAGAAGG CAATGAAGAA GAAGGGGGGA
AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATCAAAA TTCAGACGCT GACATCAGAT
CACAAATCTA AGGTCAAAGA GATTGTAGCA CAGCACACAA AGGAATGGTC AGAAATGATC
AATACCCACA GTGCTGAGGA GCAAGAAATC CGAGACCTGC ACCTCAGCCA GCAGTGTGAG
CTGCTGAAAA AGCTACTCAT CAATGCCCAC GAGCAGCAAA CCCAGCAGCT GAAACTGTCC
CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAATAGC
AAAGCCATCA GCCAAGATAA ATCTATCAAG AATAAAGCAG AACGGGAAAG GCGAGTCAGG
GAGTTAAACA GCAGCAACAC TAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCCATGAAG
CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACATCTAGA ATTCCTAGAG
AAACAGAATG AGCAGCTTTT GAAATCCTGT CATGCAGTGT CCCAAACGCA AGGCGAAGGA
GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTATG
AAACTCCAAA ATGCAAACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu93035
Clone ID Related Accession (Same CDS sequence) XM_017027882.1
Accession Version XM_017027882.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3462bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGCATCA ACGAGATCCT
CGATTGAATG AAATTTTATT TCCATTTTAT GATGCCAAAA GGGCAATGCA GATCATTGAG
ATGTATGAAC CTGATGAAGA TTTGAAGAAA AAAGGCCTTA TATCAAGTGA TGGGTTTTGC
AGATATCTGA TGTCAGATGA AAACGCCCCA GTCTTCCTAG ATCGTTTAGA ACTTTACCAA
GAAATGGACC ATCCTCTGGC TCACTACTTC ATCAGTTCTT CCCATAACAC TTATCTCACT
GGCAGACAGT TCGGCGGGAA GTCTTCGGTA GAAATGTACA GACAGGTTCT CCTGGCTGGT
TGCAGATGTG TTGAACTTGA CTGCTGGGAT GGAAAAGGTG AAGACCAAGA ACCAATAATA
ACTCATGGAA AAGCAATGTG TACAGATATC CTTTTTAAGG ATGTAATTCA AGCCATCAAG
GAAACTGCAT TTGTCACATC AGAATATCCT GTAATTCTCT CCTTTGAAAA TCACTGCAGC
AAATATCAAC AGTACAAGAT GTCCAAATAT TGCGAAGATC TATTTGGGGA TCTCCTGTTG
AAACAAGCAC TTGAATCACA TCCACTTGAA CCAGGCAGGG CTTTGCCATC CCCCAATGAC
CTCAAAAGAA AAATACTCAT AAAAAACAAG CGGCTGAAAC CTGAAGTTGA AAAAAAACAG
CTGGAAGCTT TGAGAAGCAT GATGGAAGCT GGAGAATCTG CCTCCCCAGC AAACATCTTA
GAGGACGATA ATGAAGAGGA GATCGAAAGT GCTGACCAAG AGGAGGAAGC TCACCCCGAA
TTCAAATTTG GAAATGAACT TTCTGCTGAT GACTTGGGTC ACAAGGAAGC TGTTGCAAAT
AGCGTCAAGA AGGCTTCAGA TGACCTTGAA CATGAAAACA ACAAAAAGGG CCTGGTCACT
GTAGAAGATG AGCAGGCGTG GATGGCATCT TATAAATATG TAGGTGCTAC CACTAATATC
CATCCATATT TGTCCACAAT GATCAACTAC GCCCAGCCTG TAAAGTTTCA AGGTTTCCAT
GTGGCAGAAG AACGCAATAT TCATTATAAC ATGTCTTCTT TTAATGAATC AGTCGGTCTT
GGCTACTTGA AGACACATGC AATTGAATTT GTCAATTATA ACAAACGGCA AATGAGTCGC
ATTTACCCCA AGGGAGGCCG AGTCGATTCC AGTAATTACA TGCCTCAGAT TTTCTGGAAC
GCTGGCTGCC AGATGGTTTC ACTGAACTAT CAAACCCCAG ATTTAGCGAT GCAATTGAAT
CAGGGAAAAT TTGAGTATAA TGGATCGTGC GGGTACCTTC TCAAACCAGA TTTCATGAGG
CGGCCTGATC GAACATTTGA CCCCTTCTCT GAAACTCCTG TTGATGGTGT TATTGCAGCC
ACTTGCTCAG TGCAGGTTAT ATCAGGTCAA TTCTTATCAG ATAAGAAAAT TGGCACCTAC
GTAGAGGTGG ATATGTATGG GTTGCCCACT GACACCATAC GTAAGGAATT CCGAACTCGC
ATGGTTATGA ATAATGGACT CAATCCAGTT TACAATGAAG AGTCATTTGT ATTTCGGAAG
GTGATCCTGC CGGACCTGGC TGTCTTGAGA ATAGCTGTGT ATGATGATAA CAACAAGCTG
ATTGGCCAGA GGATCCTCCC GCTTGATGGC CTCCAAGCCG GATATCGACA CATTTCCCTT
CGAAATGAGG GAAATAAACC ATTATCACTA CCAACAATTT TCTGCAATAT TGTTCTTAAA
ACATATGTGC CTGATGGATT TGGAGATATC GTGGATGCTT TATCAGATCC AAAGAAATTT
CTCTCAATTA CAGAAAAGAG AGCAGACCAA ATGAGAGCTA TGGGCATTGA AACTAGTGAC
ATAGCCGACG TGCCCAGTGA CACTTCCAAA AATGACAAGA AAGGAAAGGC CAACACCGCC
AAAGCAAATG TGACCCCTCA GAGTAGCTCT GAGCTCAGAC CAACCACCAC GGCTGCCCTG
GCCTCTGGTG TGGAAGCCAA GAAAGGTATT GAACTTATCC CTCAAGTAAG GATAGAAGAC
TTAAAGCAGA TGAAGGCTTA CTTGAAGCAT TTAAAGAAAC AGCAGAAGGA GCTAAATTCT
TTAAAGAAGA AACATGCAAA GGAACACAGT ACCATGCAGA AGTTACACTG CACGCAAGTT
GACAAAATTG TGGCACAGTA TGACAAAGAG AAGTCGACTC ATGAGAAAAT CCTAGAGAAG
GCAATGAAGA AGAAGGGGGG AAGTAATTGT CTCGAAATGA AAAAAGAAAC AGAAATCAAA
ATTCAGACGC TGACATCAGA TCACAAATCT AAGGTCAAAG AGATTGTAGC ACAGCACACA
AAGGAATGGT CAGAAATGAT CAATACCCAC AGTGCTGAGG AGCAAGAAAT CCGAGACCTG
CACCTCAGCC AGCAGTGTGA GCTGCTGAAA AAGCTACTCA TCAATGCCCA CGAGCAGCAA
ACCCAGCAGC TGAAACTGTC CCATGACAGG GAAAGCAAGG AAATGCGAGC ACACCAGGCT
AAGATTTCTA TGGAAAATAG CAAAGCCATC AGCCAAGATA AATCTATCAA GAATAAAGCA
GAACGGGAAA GGCGAGTCAG GGAGTTAAAC AGCAGCAACA CTAAAAAGTT TCTGGAAGAA
AGAAAGAGAC TTGCCATGAA GCAGTCCAAA GAAATGGATC AGTTGAAAAA AGTCCAGCTT
GAACATCTAG AATTCCTAGA GAAACAGAAT GAGCAGCTTT TGAAATCCTG TCATGCAGTG
TCCCAAACGC AAGGCGAAGG AGATGCAGCA GATGGTGAAA TTGGAAGCCG AGATGGACCG
CAGACCAGCA ACAGTAGTAT GAAACTCCAA AATGCAAACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016883371.1
CDS16..3477
Translation

Target ORF information:

RefSeq Version XM_017027882.1
Organism Homo sapiens(Human)
Definition Homo sapiens phospholipase C beta 4 (PLCB4), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017027882.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGCATCA ACGAGATCCT
CGATTGAATG AAATTTTATT TCCATTTTAT GATGCCAAAA GGGCAATGCA GATCATTGAG
ATGTATGAAC CTGATGAAGA TTTGAAGAAA AAAGGCCTTA TATCAAGTGA TGGGTTTTGC
AGATATCTGA TGTCAGATGA AAACGCCCCA GTCTTCCTAG ATCGTTTAGA ACTTTACCAA
GAAATGGACC ATCCTCTGGC TCACTACTTC ATCAGTTCTT CCCATAACAC TTATCTCACT
GGCAGACAGT TCGGCGGGAA GTCTTCGGTA GAAATGTACA GACAGGTTCT CCTGGCTGGT
TGCAGATGTG TTGAACTTGA CTGCTGGGAT GGAAAAGGTG AAGACCAAGA ACCAATAATA
ACTCATGGAA AAGCAATGTG TACAGATATC CTTTTTAAGG ATGTAATTCA AGCCATCAAG
GAAACTGCAT TTGTCACATC AGAATATCCT GTAATTCTCT CCTTTGAAAA TCACTGCAGC
AAATATCAAC AGTACAAGAT GTCCAAATAT TGCGAAGATC TATTTGGGGA TCTCCTGTTG
AAACAAGCAC TTGAATCACA TCCACTTGAA CCAGGCAGGG CTTTGCCATC CCCCAATGAC
CTCAAAAGAA AAATACTCAT AAAAAACAAG CGGCTGAAAC CTGAAGTTGA AAAAAAACAG
CTGGAAGCTT TGAGAAGCAT GATGGAAGCT GGAGAATCTG CCTCCCCAGC AAACATCTTA
GAGGACGATA ATGAAGAGGA GATCGAAAGT GCTGACCAAG AGGAGGAAGC TCACCCCGAA
TTCAAATTTG GAAATGAACT TTCTGCTGAT GACTTGGGTC ACAAGGAAGC TGTTGCAAAT
AGCGTCAAGA AGGCTTCAGA TGACCTTGAA CATGAAAACA ACAAAAAGGG CCTGGTCACT
GTAGAAGATG AGCAGGCGTG GATGGCATCT TATAAATATG TAGGTGCTAC CACTAATATC
CATCCATATT TGTCCACAAT GATCAACTAC GCCCAGCCTG TAAAGTTTCA AGGTTTCCAT
GTGGCAGAAG AACGCAATAT TCATTATAAC ATGTCTTCTT TTAATGAATC AGTCGGTCTT
GGCTACTTGA AGACACATGC AATTGAATTT GTCAATTATA ACAAACGGCA AATGAGTCGC
ATTTACCCCA AGGGAGGCCG AGTCGATTCC AGTAATTACA TGCCTCAGAT TTTCTGGAAC
GCTGGCTGCC AGATGGTTTC ACTGAACTAT CAAACCCCAG ATTTAGCGAT GCAATTGAAT
CAGGGAAAAT TTGAGTATAA TGGATCGTGC GGGTACCTTC TCAAACCAGA TTTCATGAGG
CGGCCTGATC GAACATTTGA CCCCTTCTCT GAAACTCCTG TTGATGGTGT TATTGCAGCC
ACTTGCTCAG TGCAGGTTAT ATCAGGTCAA TTCTTATCAG ATAAGAAAAT TGGCACCTAC
GTAGAGGTGG ATATGTATGG GTTGCCCACT GACACCATAC GTAAGGAATT CCGAACTCGC
ATGGTTATGA ATAATGGACT CAATCCAGTT TACAATGAAG AGTCATTTGT ATTTCGGAAG
GTGATCCTGC CGGACCTGGC TGTCTTGAGA ATAGCTGTGT ATGATGATAA CAACAAGCTG
ATTGGCCAGA GGATCCTCCC GCTTGATGGC CTCCAAGCCG GATATCGACA CATTTCCCTT
CGAAATGAGG GAAATAAACC ATTATCACTA CCAACAATTT TCTGCAATAT TGTTCTTAAA
ACATATGTGC CTGATGGATT TGGAGATATC GTGGATGCTT TATCAGATCC AAAGAAATTT
CTCTCAATTA CAGAAAAGAG AGCAGACCAA ATGAGAGCTA TGGGCATTGA AACTAGTGAC
ATAGCCGACG TGCCCAGTGA CACTTCCAAA AATGACAAGA AAGGAAAGGC CAACACCGCC
AAAGCAAATG TGACCCCTCA GAGTAGCTCT GAGCTCAGAC CAACCACCAC GGCTGCCCTG
GCCTCTGGTG TGGAAGCCAA GAAAGGTATT GAACTTATCC CTCAAGTAAG GATAGAAGAC
TTAAAGCAGA TGAAGGCTTA CTTGAAGCAT TTAAAGAAAC AGCAGAAGGA GCTAAATTCT
TTAAAGAAGA AACATGCAAA GGAACACAGT ACCATGCAGA AGTTACACTG CACGCAAGTT
GACAAAATTG TGGCACAGTA TGACAAAGAG AAGTCGACTC ATGAGAAAAT CCTAGAGAAG
GCAATGAAGA AGAAGGGGGG AAGTAATTGT CTCGAAATGA AAAAAGAAAC AGAAATCAAA
ATTCAGACGC TGACATCAGA TCACAAATCT AAGGTCAAAG AGATTGTAGC ACAGCACACA
AAGGAATGGT CAGAAATGAT CAATACCCAC AGTGCTGAGG AGCAAGAAAT CCGAGACCTG
CACCTCAGCC AGCAGTGTGA GCTGCTGAAA AAGCTACTCA TCAATGCCCA CGAGCAGCAA
ACCCAGCAGC TGAAACTGTC CCATGACAGG GAAAGCAAGG AAATGCGAGC ACACCAGGCT
AAGATTTCTA TGGAAAATAG CAAAGCCATC AGCCAAGATA AATCTATCAA GAATAAAGCA
GAACGGGAAA GGCGAGTCAG GGAGTTAAAC AGCAGCAACA CTAAAAAGTT TCTGGAAGAA
AGAAAGAGAC TTGCCATGAA GCAGTCCAAA GAAATGGATC AGTTGAAAAA AGTCCAGCTT
GAACATCTAG AATTCCTAGA GAAACAGAAT GAGCAGCTTT TGAAATCCTG TCATGCAGTG
TCCCAAACGC AAGGCGAAGG AGATGCAGCA GATGGTGAAA TTGGAAGCCG AGATGGACCG
CAGACCAGCA ACAGTAGTAT GAAACTCCAA AATGCAAACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu93036
Clone ID Related Accession (Same CDS sequence) XM_017027883.1
Accession Version XM_017027883.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3426bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGCATCA ACGAGATCCT
CGATTGAATG AAATTTTATT TCCATTTTAT GATGCCAAAA GGGCAATGCA GATCATTGAG
ATGTATGAAC CTGATGAAGA TTTGAAGAAA AAAGGCCTTA TATCAAGTGA TGGGTTTTGC
AGATATCTGA TGTCAGATGA AAACGCCCCA GTCTTCCTAG ATCGTTTAGA ACTTTACCAA
GAAATGGACC ATCCTCTGGC TCACTACTTC ATCAGTTCTT CCCATAACAC TTATCTCACT
GGCAGACAGT TCGGCGGGAA GTCTTCGGTA GAAATGTACA GACAGGTTCT CCTGGCTGGT
TGCAGATGTG TTGAACTTGA CTGCTGGGAT GGAAAAGGTG AAGACCAAGA ACCAATAATA
ACTCATGGAA AAGCAATGTG TACAGATATC CTTTTTAAGG ATGTAATTCA AGCCATCAAG
GAAACTGCAT TTGTCACATC AGAATATCCT GTAATTCTCT CCTTTGAAAA TCACTGCAGC
AAATATCAAC AGTACAAGAT GTCCAAATAT TGCGAAGATC TATTTGGGGA TCTCCTGTTG
AAACAAGCAC TTGAATCACA TCCACTTGAA CCAGGCAGGG CTTTGCCATC CCCCAATGAC
CTCAAAAGAA AAATACTCAT AAAAAACAAG CGGCTGAAAC CTGAAGTTGA AAAAAAACAG
CTGGAAGCTT TGAGAAGCAT GATGGAAGCT GGAGAATCTG CCTCCCCAGC AAACATCTTA
GAGGACGATA ATGAAGAGGA GATCGAAAGT GCTGACCAAG AGGAGGAAGC TCACCCCGAA
TTCAAATTTG GAAATGAACT TTCTGCTGAT GACTTGGGTC ACAAGGAAGC TGTTGCAAAT
AGCGTCAAGA AGGGCCTGGT CACTGTAGAA GATGAGCAGG CGTGGATGGC ATCTTATAAA
TATGTAGGTG CTACCACTAA TATCCATCCA TATTTGTCCA CAATGATCAA CTACGCCCAG
CCTGTAAAGT TTCAAGGTTT CCATGTGGCA GAAGAACGCA ATATTCATTA TAACATGTCT
TCTTTTAATG AATCAGTCGG TCTTGGCTAC TTGAAGACAC ATGCAATTGA ATTTGTCAAT
TATAACAAAC GGCAAATGAG TCGCATTTAC CCCAAGGGAG GCCGAGTCGA TTCCAGTAAT
TACATGCCTC AGATTTTCTG GAACGCTGGC TGCCAGATGG TTTCACTGAA CTATCAAACC
CCAGATTTAG CGATGCAATT GAATCAGGGA AAATTTGAGT ATAATGGATC GTGCGGGTAC
CTTCTCAAAC CAGATTTCAT GAGGCGGCCT GATCGAACAT TTGACCCCTT CTCTGAAACT
CCTGTTGATG GTGTTATTGC AGCCACTTGC TCAGTGCAGG TTATATCAGG TCAATTCTTA
TCAGATAAGA AAATTGGCAC CTACGTAGAG GTGGATATGT ATGGGTTGCC CACTGACACC
ATACGTAAGG AATTCCGAAC TCGCATGGTT ATGAATAATG GACTCAATCC AGTTTACAAT
GAAGAGTCAT TTGTATTTCG GAAGGTGATC CTGCCGGACC TGGCTGTCTT GAGAATAGCT
GTGTATGATG ATAACAACAA GCTGATTGGC CAGAGGATCC TCCCGCTTGA TGGCCTCCAA
GCCGGATATC GACACATTTC CCTTCGAAAT GAGGGAAATA AACCATTATC ACTACCAACA
ATTTTCTGCA ATATTGTTCT TAAAACATAT GTGCCTGATG GATTTGGAGA TATCGTGGAT
GCTTTATCAG ATCCAAAGAA ATTTCTCTCA ATTACAGAAA AGAGAGCAGA CCAAATGAGA
GCTATGGGCA TTGAAACTAG TGACATAGCC GACGTGCCCA GTGACACTTC CAAAAATGAC
AAGAAAGGAA AGGCCAACAC CGCCAAAGCA AATGTGACCC CTCAGAGTAG CTCTGAGCTC
AGACCAACCA CCACGGCTGC CCTGGCCTCT GGTGTGGAAG CCAAGAAAGG TATTGAACTT
ATCCCTCAAG TAAGGATAGA AGACTTAAAG CAGATGAAGG CTTACTTGAA GCATTTAAAG
AAACAGCAGA AGGAGCTAAA TTCTTTAAAG AAGAAACATG CAAAGGAACA CAGTACCATG
CAGAAGTTAC ACTGCACGCA AGTTGACAAA ATTGTGGCAC AGTATGACAA AGAGAAGTCG
ACTCATGAGA AAATCCTAGA GAAGGCAATG AAGAAGAAGG GGGGAAGTAA TTGTCTCGAA
ATGAAAAAAG AAACAGAAAT CAAAATTCAG ACGCTGACAT CAGATCACAA ATCTAAGGTC
AAAGAGATTG TAGCACAGCA CACAAAGGAA TGGTCAGAAA TGATCAATAC CCACAGTGCT
GAGGAGCAAG AAATCCGAGA CCTGCACCTC AGCCAGCAGT GTGAGCTGCT GAAAAAGCTA
CTCATCAATG CCCACGAGCA GCAAACCCAG CAGCTGAAAC TGTCCCATGA CAGGGAAAGC
AAGGAAATGC GAGCACACCA GGCTAAGATT TCTATGGAAA ATAGCAAAGC CATCAGCCAA
GATAAATCTA TCAAGAATAA AGCAGAACGG GAAAGGCGAG TCAGGGAGTT AAACAGCAGC
AACACTAAAA AGTTTCTGGA AGAAAGAAAG AGACTTGCCA TGAAGCAGTC CAAAGAAATG
GATCAGTTGA AAAAAGTCCA GCTTGAACAT CTAGAATTCC TAGAGAAACA GAATGAGCAG
CTTTTGAAAT CCTGTCATGC AGTGTCCCAA ACGCAAGGCG AAGGAGATGC AGCAGATGGT
GAAATTGGAA GCCGAGATGG ACCGCAGACC AGCAACAGTA GTATGAAACT CCAAAATGCA
AACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016883372.1
CDS273..3698
Translation

Target ORF information:

RefSeq Version XM_017027883.1
Organism Homo sapiens(Human)
Definition Homo sapiens phospholipase C beta 4 (PLCB4), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017027883.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGCATCA ACGAGATCCT
CGATTGAATG AAATTTTATT TCCATTTTAT GATGCCAAAA GGGCAATGCA GATCATTGAG
ATGTATGAAC CTGATGAAGA TTTGAAGAAA AAAGGCCTTA TATCAAGTGA TGGGTTTTGC
AGATATCTGA TGTCAGATGA AAACGCCCCA GTCTTCCTAG ATCGTTTAGA ACTTTACCAA
GAAATGGACC ATCCTCTGGC TCACTACTTC ATCAGTTCTT CCCATAACAC TTATCTCACT
GGCAGACAGT TCGGCGGGAA GTCTTCGGTA GAAATGTACA GACAGGTTCT CCTGGCTGGT
TGCAGATGTG TTGAACTTGA CTGCTGGGAT GGAAAAGGTG AAGACCAAGA ACCAATAATA
ACTCATGGAA AAGCAATGTG TACAGATATC CTTTTTAAGG ATGTAATTCA AGCCATCAAG
GAAACTGCAT TTGTCACATC AGAATATCCT GTAATTCTCT CCTTTGAAAA TCACTGCAGC
AAATATCAAC AGTACAAGAT GTCCAAATAT TGCGAAGATC TATTTGGGGA TCTCCTGTTG
AAACAAGCAC TTGAATCACA TCCACTTGAA CCAGGCAGGG CTTTGCCATC CCCCAATGAC
CTCAAAAGAA AAATACTCAT AAAAAACAAG CGGCTGAAAC CTGAAGTTGA AAAAAAACAG
CTGGAAGCTT TGAGAAGCAT GATGGAAGCT GGAGAATCTG CCTCCCCAGC AAACATCTTA
GAGGACGATA ATGAAGAGGA GATCGAAAGT GCTGACCAAG AGGAGGAAGC TCACCCCGAA
TTCAAATTTG GAAATGAACT TTCTGCTGAT GACTTGGGTC ACAAGGAAGC TGTTGCAAAT
AGCGTCAAGA AGGGCCTGGT CACTGTAGAA GATGAGCAGG CGTGGATGGC ATCTTATAAA
TATGTAGGTG CTACCACTAA TATCCATCCA TATTTGTCCA CAATGATCAA CTACGCCCAG
CCTGTAAAGT TTCAAGGTTT CCATGTGGCA GAAGAACGCA ATATTCATTA TAACATGTCT
TCTTTTAATG AATCAGTCGG TCTTGGCTAC TTGAAGACAC ATGCAATTGA ATTTGTCAAT
TATAACAAAC GGCAAATGAG TCGCATTTAC CCCAAGGGAG GCCGAGTCGA TTCCAGTAAT
TACATGCCTC AGATTTTCTG GAACGCTGGC TGCCAGATGG TTTCACTGAA CTATCAAACC
CCAGATTTAG CGATGCAATT GAATCAGGGA AAATTTGAGT ATAATGGATC GTGCGGGTAC
CTTCTCAAAC CAGATTTCAT GAGGCGGCCT GATCGAACAT TTGACCCCTT CTCTGAAACT
CCTGTTGATG GTGTTATTGC AGCCACTTGC TCAGTGCAGG TTATATCAGG TCAATTCTTA
TCAGATAAGA AAATTGGCAC CTACGTAGAG GTGGATATGT ATGGGTTGCC CACTGACACC
ATACGTAAGG AATTCCGAAC TCGCATGGTT ATGAATAATG GACTCAATCC AGTTTACAAT
GAAGAGTCAT TTGTATTTCG GAAGGTGATC CTGCCGGACC TGGCTGTCTT GAGAATAGCT
GTGTATGATG ATAACAACAA GCTGATTGGC CAGAGGATCC TCCCGCTTGA TGGCCTCCAA
GCCGGATATC GACACATTTC CCTTCGAAAT GAGGGAAATA AACCATTATC ACTACCAACA
ATTTTCTGCA ATATTGTTCT TAAAACATAT GTGCCTGATG GATTTGGAGA TATCGTGGAT
GCTTTATCAG ATCCAAAGAA ATTTCTCTCA ATTACAGAAA AGAGAGCAGA CCAAATGAGA
GCTATGGGCA TTGAAACTAG TGACATAGCC GACGTGCCCA GTGACACTTC CAAAAATGAC
AAGAAAGGAA AGGCCAACAC CGCCAAAGCA AATGTGACCC CTCAGAGTAG CTCTGAGCTC
AGACCAACCA CCACGGCTGC CCTGGCCTCT GGTGTGGAAG CCAAGAAAGG TATTGAACTT
ATCCCTCAAG TAAGGATAGA AGACTTAAAG CAGATGAAGG CTTACTTGAA GCATTTAAAG
AAACAGCAGA AGGAGCTAAA TTCTTTAAAG AAGAAACATG CAAAGGAACA CAGTACCATG
CAGAAGTTAC ACTGCACGCA AGTTGACAAA ATTGTGGCAC AGTATGACAA AGAGAAGTCG
ACTCATGAGA AAATCCTAGA GAAGGCAATG AAGAAGAAGG GGGGAAGTAA TTGTCTCGAA
ATGAAAAAAG AAACAGAAAT CAAAATTCAG ACGCTGACAT CAGATCACAA ATCTAAGGTC
AAAGAGATTG TAGCACAGCA CACAAAGGAA TGGTCAGAAA TGATCAATAC CCACAGTGCT
GAGGAGCAAG AAATCCGAGA CCTGCACCTC AGCCAGCAGT GTGAGCTGCT GAAAAAGCTA
CTCATCAATG CCCACGAGCA GCAAACCCAG CAGCTGAAAC TGTCCCATGA CAGGGAAAGC
AAGGAAATGC GAGCACACCA GGCTAAGATT TCTATGGAAA ATAGCAAAGC CATCAGCCAA
GATAAATCTA TCAAGAATAA AGCAGAACGG GAAAGGCGAG TCAGGGAGTT AAACAGCAGC
AACACTAAAA AGTTTCTGGA AGAAAGAAAG AGACTTGCCA TGAAGCAGTC CAAAGAAATG
GATCAGTTGA AAAAAGTCCA GCTTGAACAT CTAGAATTCC TAGAGAAACA GAATGAGCAG
CTTTTGAAAT CCTGTCATGC AGTGTCCCAA ACGCAAGGCG AAGGAGATGC AGCAGATGGT
GAAATTGGAA GCCGAGATGG ACCGCAGACC AGCAACAGTA GTATGAAACT CCAAAATGCA
AACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu93037
Clone ID Related Accession (Same CDS sequence) XM_017027885.1 , XM_017027884.1
Accession Version XM_017027885.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3282bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGGTGGCTG AAAATCCAGA AGTAACTAAG CAATGGGTAG AAGGCCTGAG ATCAATCATA 
CACAACTTCA GGGCCAACAA CGTCAGTCCA ATGACATGCC TCAAGAAACA CTGGATGAAA
TTGGCATTTA TGACCAACAC AAATGGTAAA ATTCCAGTTA GGAGTATTAC TAGAACATTT
GCATCGGGAA AAACAGAAAA GGTGATCTTT CAAGCACTCA AGGAGTTAGG TCTTCCCAGT
GGAAAGAATG ATGAAATTGA GCCCACAGCA TTTTCTTATG AAAAGTTCTA TGAACTGACA
CAAAAGATTT GTCCTCGGAC AGATATAGAA GATCTTTTCA AAAAAATCAA TGGAGACAAA
ACTGATTATT TAACGGTAGA CCAATTAGTG AGCTTTCTAA ATGAACATCA ACGAGATCCT
CGATTGAATG AAATTTTATT TCCATTTTAT GATGCCAAAA GGGCAATGCA GATCATTGAG
ATGTATGAAC CTGATGAAGA TTTGAAGAAA AAAGGCCTTA TATCAAGTGA TGGGTTTTGC
AGATATCTGA TGTCAGATGA AAACGCCCCA GTCTTCCTAG ATCGTTTAGA ACTTTACCAA
GAAATGGACC ATCCTCTGGC TCACTACTTC ATCAGTTCTT CCCATAACAC TTATCTCACT
GGCAGACAGT TCGGCGGGAA GTCTTCGGTA GAAATGTACA GACAGGTTCT CCTGGCTGGT
TGCAGATGTG TTGAACTTGA CTGCTGGGAT GGAAAAGGTG AAGACCAAGA ACCAATAATA
ACTCATGGAA AAGCAATGTG TACAGATATC CTTTTTAAGG ATGTAATTCA AGCCATCAAG
GAAACTGCAT TTGTCACATC AGAATATCCT GTAATTCTCT CCTTTGAAAA TCACTGCAGC
AAATATCAAC AGTACAAGAT GTCCAAATAT TGCGAAGATC TATTTGGGGA TCTCCTGTTG
AAACAAGCAC TTGAATCACA TCCACTTGAA CCAGGCAGGG CTTTGCCATC CCCCAATGAC
CTCAAAAGAA AAATACTCAT AAAAAACAAG CGGCTGAAAC CTGAAGTTGA AAAAAAACAG
CTGGAAGCTT TGAGAAGCAT GATGGAAGCT GGAGAATCTG CCTCCCCAGC AAACATCTTA
GAGGACGATA ATGAAGAGGA GATCGAAAGT GCTGACCAAG AGGAGGAAGC TCACCCCGAA
TTCAAATTTG GAAATGAACT TTCTGCTGAT GACTTGGGTC ACAAGGAAGC TGTTGCAAAT
AGCGTCAAGA AGGCTTCAGA TGACCTTGAA CATGAAAACA ACAAAAAGGG CCTGGTCACT
GTAGAAGATG AGCAGGCGTG GATGGCATCT TATAAATATG TAGGTGCTAC CACTAATATC
CATCCATATT TGTCCACAAT GATCAACTAC GCCCAGCCTG TAAAGTTTCA AGGTTTCCAT
GTGGCAGAAG AACGCAATAT TCATTATAAC ATGTCTTCTT TTAATGAATC AGTCGGTCTT
GGCTACTTGA AGACACATGC AATTGAATTT GTCAATTATA ACAAACGGCA AATGAGTCGC
ATTTACCCCA AGGGAGGCCG AGTCGATTCC AGTAATTACA TGCCTCAGAT TTTCTGGAAC
GCTGGCTGCC AGATGGTTTC ACTGAACTAT CAAACCCCAG ATTTAGCGAT GCAATTGAAT
CAGGGAAAAT TTGAGTATAA TGGATCGTGC GGGTACCTTC TCAAACCAGA TTTCATGAGG
CGGCCTGATC GAACATTTGA CCCCTTCTCT GAAACTCCTG TTGATGGTGT TATTGCAGCC
ACTTGCTCAG TGCAGGTTAT ATCAGGTCAA TTCTTATCAG ATAAGAAAAT TGGCACCTAC
GTAGAGGTGG ATATGTATGG GTTGCCCACT GACACCATAC GTAAGGAATT CCGAACTCGC
ATGGTTATGA ATAATGGACT CAATCCAGTT TACAATGAAG AGTCATTTGT ATTTCGGAAG
GTGATCCTGC CGGACCTGGC TGTCTTGAGA ATAGCTGTGT ATGATGATAA CAACAAGCTG
ATTGGCCAGA GGATCCTCCC GCTTGATGGC CTCCAAGCCG GATATCGACA CATTTCCCTT
CGAAATGAGG GAAATAAACC ATTATCACTA CCAACAATTT TCTGCAATAT TGTTCTTAAA
ACATATGTGC CTGATGGATT TGGAGATATC GTGGATGCTT TATCAGATCC AAAGAAATTT
CTCTCAATTA CAGAAAAGAG AGCAGACCAA ATGAGAGCTA TGGGCATTGA AACTAGTGAC
ATAGCCGACG TGCCCAGTGA CACTTCCAAA AATGACAAGA AAGGAAAGGC CAACACCGCC
AAAGCAAATG TGACCCCTCA GAGTAGCTCT GAGCTCAGAC CAACCACCAC GGCTGCCCTG
GCCTCTGGTG TGGAAGCCAA GAAAGGTATT GAACTTATCC CTCAAGTAAG GATAGAAGAC
TTAAAGCAGA TGAAGGCTTA CTTGAAGCAT TTAAAGAAAC AGCAGAAGGA GCTAAATTCT
TTAAAGAAGA AACATGCAAA GGAACACAGT ACCATGCAGA AGTTACACTG CACGCAAGTT
GACAAAATTG TGGCACAGTA TGACAAAGAG AAGTCGACTC ATGAGAAAAT CCTAGAGAAG
GCAATGAAGA AGAAGGGGGG AAGTAATTGT CTCGAAATGA AAAAAGAAAC AGAAATCAAA
ATTCAGACGC TGACATCAGA TCACAAATCT AAGGTCAAAG AGATTGTAGC ACAGCACACA
AAGGAATGGT CAGAAATGAT CAATACCCAC AGTGCTGAGG AGCAAGAAAT CCGAGACCTG
CACCTCAGCC AGCAGTGTGA GCTGCTGAAA AAGCTACTCA TCAATGCCCA CGAGCAGCAA
ACCCAGCAGC TGAAACTGTC CCATGACAGG GAAAGCAAGG AAATGCGAGC ACACCAGGCT
AAGATTTCTA TGGAAAATAG CAAAGCCATC AGCCAAGATA AATCTATCAA GAATAAAGCA
GAACGGGAAA GGCGAGTCAG GGAGTTAAAC AGCAGCAACA CTAAAAAGTT TCTGGAAGAA
AGAAAGAGAC TTGCCATGAA GCAGTCCAAA GAAATGGATC AGTTGAAAAA AGTCCAGCTT
GAACATCTAG AATTCCTAGA GAAACAGAAT GAGCAGCTTT TGAAATCCTG TCATGCAGTG
TCCCAAACGC AAGGCGAAGG AGATGCAGCA GATGGTGAAA TTGGAAGCCG AGATGGACCG
CAGACCAGCA ACAGTAGTAT GAAACTCCAA AATGCAAACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016883374.1
CDS323..3604
Translation

Target ORF information:

RefSeq Version XM_017027885.1
Organism Homo sapiens(Human)
Definition Homo sapiens phospholipase C beta 4 (PLCB4), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017027885.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGGTGGCTG AAAATCCAGA AGTAACTAAG CAATGGGTAG AAGGCCTGAG ATCAATCATA 
CACAACTTCA GGGCCAACAA CGTCAGTCCA ATGACATGCC TCAAGAAACA CTGGATGAAA
TTGGCATTTA TGACCAACAC AAATGGTAAA ATTCCAGTTA GGAGTATTAC TAGAACATTT
GCATCGGGAA AAACAGAAAA GGTGATCTTT CAAGCACTCA AGGAGTTAGG TCTTCCCAGT
GGAAAGAATG ATGAAATTGA GCCCACAGCA TTTTCTTATG AAAAGTTCTA TGAACTGACA
CAAAAGATTT GTCCTCGGAC AGATATAGAA GATCTTTTCA AAAAAATCAA TGGAGACAAA
ACTGATTATT TAACGGTAGA CCAATTAGTG AGCTTTCTAA ATGAACATCA ACGAGATCCT
CGATTGAATG AAATTTTATT TCCATTTTAT GATGCCAAAA GGGCAATGCA GATCATTGAG
ATGTATGAAC CTGATGAAGA TTTGAAGAAA AAAGGCCTTA TATCAAGTGA TGGGTTTTGC
AGATATCTGA TGTCAGATGA AAACGCCCCA GTCTTCCTAG ATCGTTTAGA ACTTTACCAA
GAAATGGACC ATCCTCTGGC TCACTACTTC ATCAGTTCTT CCCATAACAC TTATCTCACT
GGCAGACAGT TCGGCGGGAA GTCTTCGGTA GAAATGTACA GACAGGTTCT CCTGGCTGGT
TGCAGATGTG TTGAACTTGA CTGCTGGGAT GGAAAAGGTG AAGACCAAGA ACCAATAATA
ACTCATGGAA AAGCAATGTG TACAGATATC CTTTTTAAGG ATGTAATTCA AGCCATCAAG
GAAACTGCAT TTGTCACATC AGAATATCCT GTAATTCTCT CCTTTGAAAA TCACTGCAGC
AAATATCAAC AGTACAAGAT GTCCAAATAT TGCGAAGATC TATTTGGGGA TCTCCTGTTG
AAACAAGCAC TTGAATCACA TCCACTTGAA CCAGGCAGGG CTTTGCCATC CCCCAATGAC
CTCAAAAGAA AAATACTCAT AAAAAACAAG CGGCTGAAAC CTGAAGTTGA AAAAAAACAG
CTGGAAGCTT TGAGAAGCAT GATGGAAGCT GGAGAATCTG CCTCCCCAGC AAACATCTTA
GAGGACGATA ATGAAGAGGA GATCGAAAGT GCTGACCAAG AGGAGGAAGC TCACCCCGAA
TTCAAATTTG GAAATGAACT TTCTGCTGAT GACTTGGGTC ACAAGGAAGC TGTTGCAAAT
AGCGTCAAGA AGGCTTCAGA TGACCTTGAA CATGAAAACA ACAAAAAGGG CCTGGTCACT
GTAGAAGATG AGCAGGCGTG GATGGCATCT TATAAATATG TAGGTGCTAC CACTAATATC
CATCCATATT TGTCCACAAT GATCAACTAC GCCCAGCCTG TAAAGTTTCA AGGTTTCCAT
GTGGCAGAAG AACGCAATAT TCATTATAAC ATGTCTTCTT TTAATGAATC AGTCGGTCTT
GGCTACTTGA AGACACATGC AATTGAATTT GTCAATTATA ACAAACGGCA AATGAGTCGC
ATTTACCCCA AGGGAGGCCG AGTCGATTCC AGTAATTACA TGCCTCAGAT TTTCTGGAAC
GCTGGCTGCC AGATGGTTTC ACTGAACTAT CAAACCCCAG ATTTAGCGAT GCAATTGAAT
CAGGGAAAAT TTGAGTATAA TGGATCGTGC GGGTACCTTC TCAAACCAGA TTTCATGAGG
CGGCCTGATC GAACATTTGA CCCCTTCTCT GAAACTCCTG TTGATGGTGT TATTGCAGCC
ACTTGCTCAG TGCAGGTTAT ATCAGGTCAA TTCTTATCAG ATAAGAAAAT TGGCACCTAC
GTAGAGGTGG ATATGTATGG GTTGCCCACT GACACCATAC GTAAGGAATT CCGAACTCGC
ATGGTTATGA ATAATGGACT CAATCCAGTT TACAATGAAG AGTCATTTGT ATTTCGGAAG
GTGATCCTGC CGGACCTGGC TGTCTTGAGA ATAGCTGTGT ATGATGATAA CAACAAGCTG
ATTGGCCAGA GGATCCTCCC GCTTGATGGC CTCCAAGCCG GATATCGACA CATTTCCCTT
CGAAATGAGG GAAATAAACC ATTATCACTA CCAACAATTT TCTGCAATAT TGTTCTTAAA
ACATATGTGC CTGATGGATT TGGAGATATC GTGGATGCTT TATCAGATCC AAAGAAATTT
CTCTCAATTA CAGAAAAGAG AGCAGACCAA ATGAGAGCTA TGGGCATTGA AACTAGTGAC
ATAGCCGACG TGCCCAGTGA CACTTCCAAA AATGACAAGA AAGGAAAGGC CAACACCGCC
AAAGCAAATG TGACCCCTCA GAGTAGCTCT GAGCTCAGAC CAACCACCAC GGCTGCCCTG
GCCTCTGGTG TGGAAGCCAA GAAAGGTATT GAACTTATCC CTCAAGTAAG GATAGAAGAC
TTAAAGCAGA TGAAGGCTTA CTTGAAGCAT TTAAAGAAAC AGCAGAAGGA GCTAAATTCT
TTAAAGAAGA AACATGCAAA GGAACACAGT ACCATGCAGA AGTTACACTG CACGCAAGTT
GACAAAATTG TGGCACAGTA TGACAAAGAG AAGTCGACTC ATGAGAAAAT CCTAGAGAAG
GCAATGAAGA AGAAGGGGGG AAGTAATTGT CTCGAAATGA AAAAAGAAAC AGAAATCAAA
ATTCAGACGC TGACATCAGA TCACAAATCT AAGGTCAAAG AGATTGTAGC ACAGCACACA
AAGGAATGGT CAGAAATGAT CAATACCCAC AGTGCTGAGG AGCAAGAAAT CCGAGACCTG
CACCTCAGCC AGCAGTGTGA GCTGCTGAAA AAGCTACTCA TCAATGCCCA CGAGCAGCAA
ACCCAGCAGC TGAAACTGTC CCATGACAGG GAAAGCAAGG AAATGCGAGC ACACCAGGCT
AAGATTTCTA TGGAAAATAG CAAAGCCATC AGCCAAGATA AATCTATCAA GAATAAAGCA
GAACGGGAAA GGCGAGTCAG GGAGTTAAAC AGCAGCAACA CTAAAAAGTT TCTGGAAGAA
AGAAAGAGAC TTGCCATGAA GCAGTCCAAA GAAATGGATC AGTTGAAAAA AGTCCAGCTT
GAACATCTAG AATTCCTAGA GAAACAGAAT GAGCAGCTTT TGAAATCCTG TCATGCAGTG
TCCCAAACGC AAGGCGAAGG AGATGCAGCA GATGGTGAAA TTGGAAGCCG AGATGGACCG
CAGACCAGCA ACAGTAGTAT GAAACTCCAA AATGCAAACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu93037
Clone ID Related Accession (Same CDS sequence) XM_017027885.1 , XM_017027884.1
Accession Version XM_017027884.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3282bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGGTGGCTG AAAATCCAGA AGTAACTAAG CAATGGGTAG AAGGCCTGAG ATCAATCATA 
CACAACTTCA GGGCCAACAA CGTCAGTCCA ATGACATGCC TCAAGAAACA CTGGATGAAA
TTGGCATTTA TGACCAACAC AAATGGTAAA ATTCCAGTTA GGAGTATTAC TAGAACATTT
GCATCGGGAA AAACAGAAAA GGTGATCTTT CAAGCACTCA AGGAGTTAGG TCTTCCCAGT
GGAAAGAATG ATGAAATTGA GCCCACAGCA TTTTCTTATG AAAAGTTCTA TGAACTGACA
CAAAAGATTT GTCCTCGGAC AGATATAGAA GATCTTTTCA AAAAAATCAA TGGAGACAAA
ACTGATTATT TAACGGTAGA CCAATTAGTG AGCTTTCTAA ATGAACATCA ACGAGATCCT
CGATTGAATG AAATTTTATT TCCATTTTAT GATGCCAAAA GGGCAATGCA GATCATTGAG
ATGTATGAAC CTGATGAAGA TTTGAAGAAA AAAGGCCTTA TATCAAGTGA TGGGTTTTGC
AGATATCTGA TGTCAGATGA AAACGCCCCA GTCTTCCTAG ATCGTTTAGA ACTTTACCAA
GAAATGGACC ATCCTCTGGC TCACTACTTC ATCAGTTCTT CCCATAACAC TTATCTCACT
GGCAGACAGT TCGGCGGGAA GTCTTCGGTA GAAATGTACA GACAGGTTCT CCTGGCTGGT
TGCAGATGTG TTGAACTTGA CTGCTGGGAT GGAAAAGGTG AAGACCAAGA ACCAATAATA
ACTCATGGAA AAGCAATGTG TACAGATATC CTTTTTAAGG ATGTAATTCA AGCCATCAAG
GAAACTGCAT TTGTCACATC AGAATATCCT GTAATTCTCT CCTTTGAAAA TCACTGCAGC
AAATATCAAC AGTACAAGAT GTCCAAATAT TGCGAAGATC TATTTGGGGA TCTCCTGTTG
AAACAAGCAC TTGAATCACA TCCACTTGAA CCAGGCAGGG CTTTGCCATC CCCCAATGAC
CTCAAAAGAA AAATACTCAT AAAAAACAAG CGGCTGAAAC CTGAAGTTGA AAAAAAACAG
CTGGAAGCTT TGAGAAGCAT GATGGAAGCT GGAGAATCTG CCTCCCCAGC AAACATCTTA
GAGGACGATA ATGAAGAGGA GATCGAAAGT GCTGACCAAG AGGAGGAAGC TCACCCCGAA
TTCAAATTTG GAAATGAACT TTCTGCTGAT GACTTGGGTC ACAAGGAAGC TGTTGCAAAT
AGCGTCAAGA AGGCTTCAGA TGACCTTGAA CATGAAAACA ACAAAAAGGG CCTGGTCACT
GTAGAAGATG AGCAGGCGTG GATGGCATCT TATAAATATG TAGGTGCTAC CACTAATATC
CATCCATATT TGTCCACAAT GATCAACTAC GCCCAGCCTG TAAAGTTTCA AGGTTTCCAT
GTGGCAGAAG AACGCAATAT TCATTATAAC ATGTCTTCTT TTAATGAATC AGTCGGTCTT
GGCTACTTGA AGACACATGC AATTGAATTT GTCAATTATA ACAAACGGCA AATGAGTCGC
ATTTACCCCA AGGGAGGCCG AGTCGATTCC AGTAATTACA TGCCTCAGAT TTTCTGGAAC
GCTGGCTGCC AGATGGTTTC ACTGAACTAT CAAACCCCAG ATTTAGCGAT GCAATTGAAT
CAGGGAAAAT TTGAGTATAA TGGATCGTGC GGGTACCTTC TCAAACCAGA TTTCATGAGG
CGGCCTGATC GAACATTTGA CCCCTTCTCT GAAACTCCTG TTGATGGTGT TATTGCAGCC
ACTTGCTCAG TGCAGGTTAT ATCAGGTCAA TTCTTATCAG ATAAGAAAAT TGGCACCTAC
GTAGAGGTGG ATATGTATGG GTTGCCCACT GACACCATAC GTAAGGAATT CCGAACTCGC
ATGGTTATGA ATAATGGACT CAATCCAGTT TACAATGAAG AGTCATTTGT ATTTCGGAAG
GTGATCCTGC CGGACCTGGC TGTCTTGAGA ATAGCTGTGT ATGATGATAA CAACAAGCTG
ATTGGCCAGA GGATCCTCCC GCTTGATGGC CTCCAAGCCG GATATCGACA CATTTCCCTT
CGAAATGAGG GAAATAAACC ATTATCACTA CCAACAATTT TCTGCAATAT TGTTCTTAAA
ACATATGTGC CTGATGGATT TGGAGATATC GTGGATGCTT TATCAGATCC AAAGAAATTT
CTCTCAATTA CAGAAAAGAG AGCAGACCAA ATGAGAGCTA TGGGCATTGA AACTAGTGAC
ATAGCCGACG TGCCCAGTGA CACTTCCAAA AATGACAAGA AAGGAAAGGC CAACACCGCC
AAAGCAAATG TGACCCCTCA GAGTAGCTCT GAGCTCAGAC CAACCACCAC GGCTGCCCTG
GCCTCTGGTG TGGAAGCCAA GAAAGGTATT GAACTTATCC CTCAAGTAAG GATAGAAGAC
TTAAAGCAGA TGAAGGCTTA CTTGAAGCAT TTAAAGAAAC AGCAGAAGGA GCTAAATTCT
TTAAAGAAGA AACATGCAAA GGAACACAGT ACCATGCAGA AGTTACACTG CACGCAAGTT
GACAAAATTG TGGCACAGTA TGACAAAGAG AAGTCGACTC ATGAGAAAAT CCTAGAGAAG
GCAATGAAGA AGAAGGGGGG AAGTAATTGT CTCGAAATGA AAAAAGAAAC AGAAATCAAA
ATTCAGACGC TGACATCAGA TCACAAATCT AAGGTCAAAG AGATTGTAGC ACAGCACACA
AAGGAATGGT CAGAAATGAT CAATACCCAC AGTGCTGAGG AGCAAGAAAT CCGAGACCTG
CACCTCAGCC AGCAGTGTGA GCTGCTGAAA AAGCTACTCA TCAATGCCCA CGAGCAGCAA
ACCCAGCAGC TGAAACTGTC CCATGACAGG GAAAGCAAGG AAATGCGAGC ACACCAGGCT
AAGATTTCTA TGGAAAATAG CAAAGCCATC AGCCAAGATA AATCTATCAA GAATAAAGCA
GAACGGGAAA GGCGAGTCAG GGAGTTAAAC AGCAGCAACA CTAAAAAGTT TCTGGAAGAA
AGAAAGAGAC TTGCCATGAA GCAGTCCAAA GAAATGGATC AGTTGAAAAA AGTCCAGCTT
GAACATCTAG AATTCCTAGA GAAACAGAAT GAGCAGCTTT TGAAATCCTG TCATGCAGTG
TCCCAAACGC AAGGCGAAGG AGATGCAGCA GATGGTGAAA TTGGAAGCCG AGATGGACCG
CAGACCAGCA ACAGTAGTAT GAAACTCCAA AATGCAAACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016883373.1
CDS646..3927
Translation

Target ORF information:

RefSeq Version XM_017027884.1
Organism Homo sapiens(Human)
Definition Homo sapiens phospholipase C beta 4 (PLCB4), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017027884.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGGTGGCTG AAAATCCAGA AGTAACTAAG CAATGGGTAG AAGGCCTGAG ATCAATCATA 
CACAACTTCA GGGCCAACAA CGTCAGTCCA ATGACATGCC TCAAGAAACA CTGGATGAAA
TTGGCATTTA TGACCAACAC AAATGGTAAA ATTCCAGTTA GGAGTATTAC TAGAACATTT
GCATCGGGAA AAACAGAAAA GGTGATCTTT CAAGCACTCA AGGAGTTAGG TCTTCCCAGT
GGAAAGAATG ATGAAATTGA GCCCACAGCA TTTTCTTATG AAAAGTTCTA TGAACTGACA
CAAAAGATTT GTCCTCGGAC AGATATAGAA GATCTTTTCA AAAAAATCAA TGGAGACAAA
ACTGATTATT TAACGGTAGA CCAATTAGTG AGCTTTCTAA ATGAACATCA ACGAGATCCT
CGATTGAATG AAATTTTATT TCCATTTTAT GATGCCAAAA GGGCAATGCA GATCATTGAG
ATGTATGAAC CTGATGAAGA TTTGAAGAAA AAAGGCCTTA TATCAAGTGA TGGGTTTTGC
AGATATCTGA TGTCAGATGA AAACGCCCCA GTCTTCCTAG ATCGTTTAGA ACTTTACCAA
GAAATGGACC ATCCTCTGGC TCACTACTTC ATCAGTTCTT CCCATAACAC TTATCTCACT
GGCAGACAGT TCGGCGGGAA GTCTTCGGTA GAAATGTACA GACAGGTTCT CCTGGCTGGT
TGCAGATGTG TTGAACTTGA CTGCTGGGAT GGAAAAGGTG AAGACCAAGA ACCAATAATA
ACTCATGGAA AAGCAATGTG TACAGATATC CTTTTTAAGG ATGTAATTCA AGCCATCAAG
GAAACTGCAT TTGTCACATC AGAATATCCT GTAATTCTCT CCTTTGAAAA TCACTGCAGC
AAATATCAAC AGTACAAGAT GTCCAAATAT TGCGAAGATC TATTTGGGGA TCTCCTGTTG
AAACAAGCAC TTGAATCACA TCCACTTGAA CCAGGCAGGG CTTTGCCATC CCCCAATGAC
CTCAAAAGAA AAATACTCAT AAAAAACAAG CGGCTGAAAC CTGAAGTTGA AAAAAAACAG
CTGGAAGCTT TGAGAAGCAT GATGGAAGCT GGAGAATCTG CCTCCCCAGC AAACATCTTA
GAGGACGATA ATGAAGAGGA GATCGAAAGT GCTGACCAAG AGGAGGAAGC TCACCCCGAA
TTCAAATTTG GAAATGAACT TTCTGCTGAT GACTTGGGTC ACAAGGAAGC TGTTGCAAAT
AGCGTCAAGA AGGCTTCAGA TGACCTTGAA CATGAAAACA ACAAAAAGGG CCTGGTCACT
GTAGAAGATG AGCAGGCGTG GATGGCATCT TATAAATATG TAGGTGCTAC CACTAATATC
CATCCATATT TGTCCACAAT GATCAACTAC GCCCAGCCTG TAAAGTTTCA AGGTTTCCAT
GTGGCAGAAG AACGCAATAT TCATTATAAC ATGTCTTCTT TTAATGAATC AGTCGGTCTT
GGCTACTTGA AGACACATGC AATTGAATTT GTCAATTATA ACAAACGGCA AATGAGTCGC
ATTTACCCCA AGGGAGGCCG AGTCGATTCC AGTAATTACA TGCCTCAGAT TTTCTGGAAC
GCTGGCTGCC AGATGGTTTC ACTGAACTAT CAAACCCCAG ATTTAGCGAT GCAATTGAAT
CAGGGAAAAT TTGAGTATAA TGGATCGTGC GGGTACCTTC TCAAACCAGA TTTCATGAGG
CGGCCTGATC GAACATTTGA CCCCTTCTCT GAAACTCCTG TTGATGGTGT TATTGCAGCC
ACTTGCTCAG TGCAGGTTAT ATCAGGTCAA TTCTTATCAG ATAAGAAAAT TGGCACCTAC
GTAGAGGTGG ATATGTATGG GTTGCCCACT GACACCATAC GTAAGGAATT CCGAACTCGC
ATGGTTATGA ATAATGGACT CAATCCAGTT TACAATGAAG AGTCATTTGT ATTTCGGAAG
GTGATCCTGC CGGACCTGGC TGTCTTGAGA ATAGCTGTGT ATGATGATAA CAACAAGCTG
ATTGGCCAGA GGATCCTCCC GCTTGATGGC CTCCAAGCCG GATATCGACA CATTTCCCTT
CGAAATGAGG GAAATAAACC ATTATCACTA CCAACAATTT TCTGCAATAT TGTTCTTAAA
ACATATGTGC CTGATGGATT TGGAGATATC GTGGATGCTT TATCAGATCC AAAGAAATTT
CTCTCAATTA CAGAAAAGAG AGCAGACCAA ATGAGAGCTA TGGGCATTGA AACTAGTGAC
ATAGCCGACG TGCCCAGTGA CACTTCCAAA AATGACAAGA AAGGAAAGGC CAACACCGCC
AAAGCAAATG TGACCCCTCA GAGTAGCTCT GAGCTCAGAC CAACCACCAC GGCTGCCCTG
GCCTCTGGTG TGGAAGCCAA GAAAGGTATT GAACTTATCC CTCAAGTAAG GATAGAAGAC
TTAAAGCAGA TGAAGGCTTA CTTGAAGCAT TTAAAGAAAC AGCAGAAGGA GCTAAATTCT
TTAAAGAAGA AACATGCAAA GGAACACAGT ACCATGCAGA AGTTACACTG CACGCAAGTT
GACAAAATTG TGGCACAGTA TGACAAAGAG AAGTCGACTC ATGAGAAAAT CCTAGAGAAG
GCAATGAAGA AGAAGGGGGG AAGTAATTGT CTCGAAATGA AAAAAGAAAC AGAAATCAAA
ATTCAGACGC TGACATCAGA TCACAAATCT AAGGTCAAAG AGATTGTAGC ACAGCACACA
AAGGAATGGT CAGAAATGAT CAATACCCAC AGTGCTGAGG AGCAAGAAAT CCGAGACCTG
CACCTCAGCC AGCAGTGTGA GCTGCTGAAA AAGCTACTCA TCAATGCCCA CGAGCAGCAA
ACCCAGCAGC TGAAACTGTC CCATGACAGG GAAAGCAAGG AAATGCGAGC ACACCAGGCT
AAGATTTCTA TGGAAAATAG CAAAGCCATC AGCCAAGATA AATCTATCAA GAATAAAGCA
GAACGGGAAA GGCGAGTCAG GGAGTTAAAC AGCAGCAACA CTAAAAAGTT TCTGGAAGAA
AGAAAGAGAC TTGCCATGAA GCAGTCCAAA GAAATGGATC AGTTGAAAAA AGTCCAGCTT
GAACATCTAG AATTCCTAGA GAAACAGAAT GAGCAGCTTT TGAAATCCTG TCATGCAGTG
TCCCAAACGC AAGGCGAAGG AGATGCAGCA GATGGTGAAA TTGGAAGCCG AGATGGACCG
CAGACCAGCA ACAGTAGTAT GAAACTCCAA AATGCAAACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14145
Clone ID Related Accession (Same CDS sequence) NM_000933.3 , XM_017027880.1
Accession Version NM_000933.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3585bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-17
Organism Homo sapiens(Human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform a
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB270238.1, BX648680.1, BC117458.1, DR761451.1 and AL117633.1. On Feb 24, 2010 this sequence version replaced NM_000933.2. Transcript Variant: This variant (1) lacks an alternate in-frame exon in the central coding region and includes an alternate exon in the 3' coding region that results in a frameshift, compared to variant 3. The complete exon combination of the 5' UTR for this variant has not been determined. The resulting isoform (a) lacks an internal segment and has a longer and distinct C-terminus, compared to isoform c. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGGCCTGGTC
ACTGTAGAAG ATGAGCAGGC GTGGATGGCA TCTTATAAAT ATGTAGGTGC TACCACTAAT
ATCCATCCAT ATTTGTCCAC AATGATCAAC TACGCCCAGC CTGTAAAGTT TCAAGGTTTC
CATGTGGCAG AAGAACGCAA TATTCATTAT AACATGTCTT CTTTTAATGA ATCAGTCGGT
CTTGGCTACT TGAAGACACA TGCAATTGAA TTTGTCAATT ATAACAAACG GCAAATGAGT
CGCATTTACC CCAAGGGAGG CCGAGTCGAT TCCAGTAATT ACATGCCTCA GATTTTCTGG
AACGCTGGCT GCCAGATGGT TTCACTGAAC TATCAAACCC CAGATTTAGC GATGCAATTG
AATCAGGGAA AATTTGAGTA TAATGGATCG TGCGGGTACC TTCTCAAACC AGATTTCATG
AGGCGGCCTG ATCGAACATT TGACCCCTTC TCTGAAACTC CTGTTGATGG TGTTATTGCA
GCCACTTGCT CAGTGCAGGT TATATCAGGT CAATTCTTAT CAGATAAGAA AATTGGCACC
TACGTAGAGG TGGATATGTA TGGGTTGCCC ACTGACACCA TACGTAAGGA ATTCCGAACT
CGCATGGTTA TGAATAATGG ACTCAATCCA GTTTACAATG AAGAGTCATT TGTATTTCGG
AAGGTGATCC TGCCGGACCT GGCTGTCTTG AGAATAGCTG TGTATGATGA TAACAACAAG
CTGATTGGCC AGAGGATCCT CCCGCTTGAT GGCCTCCAAG CCGGATATCG ACACATTTCC
CTTCGAAATG AGGGAAATAA ACCATTATCA CTACCAACAA TTTTCTGCAA TATTGTTCTT
AAAACATATG TGCCTGATGG ATTTGGAGAT ATCGTGGATG CTTTATCAGA TCCAAAGAAA
TTTCTCTCAA TTACAGAAAA GAGAGCAGAC CAAATGAGAG CTATGGGCAT TGAAACTAGT
GACATAGCCG ACGTGCCCAG TGACACTTCC AAAAATGACA AGAAAGGAAA GGCCAACACC
GCCAAAGCAA ATGTGACCCC TCAGAGTAGC TCTGAGCTCA GACCAACCAC CACGGCTGCC
CTGGCCTCTG GTGTGGAAGC CAAGAAAGGT ATTGAACTTA TCCCTCAAGT AAGGATAGAA
GACTTAAAGC AGATGAAGGC TTACTTGAAG CATTTAAAGA AACAGCAGAA GGAGCTAAAT
TCTTTAAAGA AGAAACATGC AAAGGAACAC AGTACCATGC AGAAGTTACA CTGCACGCAA
GTTGACAAAA TTGTGGCACA GTATGACAAA GAGAAGTCGA CTCATGAGAA AATCCTAGAG
AAGGCAATGA AGAAGAAGGG GGGAAGTAAT TGTCTCGAAA TGAAAAAAGA AACAGAAATC
AAAATTCAGA CGCTGACATC AGATCACAAA TCTAAGGTCA AAGAGATTGT AGCACAGCAC
ACAAAGGAAT GGTCAGAAAT GATCAATACC CACAGTGCTG AGGAGCAAGA AATCCGAGAC
CTGCACCTCA GCCAGCAGTG TGAGCTGCTG AAAAAGCTAC TCATCAATGC CCACGAGCAG
CAAACCCAGC AGCTGAAACT GTCCCATGAC AGGGAAAGCA AGGAAATGCG AGCACACCAG
GCTAAGATTT CTATGGAAAA TAGCAAAGCC ATCAGCCAAG ATAAATCTAT CAAGAATAAA
GCAGAACGGG AAAGGCGAGT CAGGGAGTTA AACAGCAGCA ACACTAAAAA GTTTCTGGAA
GAAAGAAAGA GACTTGCCAT GAAGCAGTCC AAAGAAATGG ATCAGTTGAA AAAAGTCCAG
CTTGAACATC TAGAATTCCT AGAGAAACAG AATGAGCAGC TTTTGAAATC CTGTCATGCA
GTGTCCCAAA CGCAAGGCGA AGGAGATGCA GCAGATGGTG AAATTGGAAG CCGAGATGGA
CCGCAGACCA GCAACAGTAG TATGAAACTC CAAAATGCAA ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000924.3
CDS16..3600
Misc Feature(1)19..21
Misc Feature(2)64..444
Misc Feature(3)order(79..81,85..87,121..123,172..174,364..366)
Misc Feature(4)order(88..90,94..96,112..114,118..120,124..126,169..174)
Misc Feature(5)667..2436
Misc Feature(6)676..951
Misc Feature(7)949..2019
Misc Feature(8)order(997..1002,1087..1089,1093..1095,1138..1140,1240..1242,1396..1398,1402..1404,1795..1797,1876..1878,1882..1884)
Misc Feature(9)order(997..999,1138..1140)
Misc Feature(10)order(1000..1002,1087..1089,1093..1095,1240..1242)
Misc Feature(11)2119..2469
Misc Feature(12)order(2170..2172,2248..2250,2341..2343,2347..2349,2353..2355,2359..2361)
Misc Feature(13)2671..2673
Misc Feature(14)2755..2877
Misc Feature(15)2800..3513
Exon (1)1..99
Exon (2)100..180
Exon (3)181..240
Exon (4)241..384
Exon (5)385..464
Exon (6)465..518
Exon (7)519..600
Exon (8)601..701
Exon (9)702..759
Exon (10)760..868
Exon (11)869..1079
Exon (12)1080..1173
Exon (13)1174..1253
Exon (14)1254..1338
Exon (15)1339..1429
Exon (16)1430..1525
Exon (17)1526..1626
Exon (18)1627..1768
Exon (19)1769..1853
Exon (20)1854..1978
Exon (21)1979..2030
Exon (22)2031..2133
Exon (23)2134..2298
Exon (24)2299..2503
Exon (25)2504..2592
Exon (26)2593..2743
Exon (27)2744..2793
Exon (28)2794..2859
Exon (29)2860..2975
Exon (30)2976..3051
Exon (31)3052..3227
Exon (32)3228..3329
Exon (33)3330..3387
Exon (34)3388..3474
Exon (35)3475..3511
Exon (36)3512..5407
Translation

Target ORF information:

RefSeq Version NM_000933.3
Organism Homo sapiens(Human)
Definition Homo sapiens phospholipase C beta 4 (PLCB4), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000933.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGGCCTGGTC
ACTGTAGAAG ATGAGCAGGC GTGGATGGCA TCTTATAAAT ATGTAGGTGC TACCACTAAT
ATCCATCCAT ATTTGTCCAC AATGATCAAC TACGCCCAGC CTGTAAAGTT TCAAGGTTTC
CATGTGGCAG AAGAACGCAA TATTCATTAT AACATGTCTT CTTTTAATGA ATCAGTCGGT
CTTGGCTACT TGAAGACACA TGCAATTGAA TTTGTCAATT ATAACAAACG GCAAATGAGT
CGCATTTACC CCAAGGGAGG CCGAGTCGAT TCCAGTAATT ACATGCCTCA GATTTTCTGG
AACGCTGGCT GCCAGATGGT TTCACTGAAC TATCAAACCC CAGATTTAGC GATGCAATTG
AATCAGGGAA AATTTGAGTA TAATGGATCG TGCGGGTACC TTCTCAAACC AGATTTCATG
AGGCGGCCTG ATCGAACATT TGACCCCTTC TCTGAAACTC CTGTTGATGG TGTTATTGCA
GCCACTTGCT CAGTGCAGGT TATATCAGGT CAATTCTTAT CAGATAAGAA AATTGGCACC
TACGTAGAGG TGGATATGTA TGGGTTGCCC ACTGACACCA TACGTAAGGA ATTCCGAACT
CGCATGGTTA TGAATAATGG ACTCAATCCA GTTTACAATG AAGAGTCATT TGTATTTCGG
AAGGTGATCC TGCCGGACCT GGCTGTCTTG AGAATAGCTG TGTATGATGA TAACAACAAG
CTGATTGGCC AGAGGATCCT CCCGCTTGAT GGCCTCCAAG CCGGATATCG ACACATTTCC
CTTCGAAATG AGGGAAATAA ACCATTATCA CTACCAACAA TTTTCTGCAA TATTGTTCTT
AAAACATATG TGCCTGATGG ATTTGGAGAT ATCGTGGATG CTTTATCAGA TCCAAAGAAA
TTTCTCTCAA TTACAGAAAA GAGAGCAGAC CAAATGAGAG CTATGGGCAT TGAAACTAGT
GACATAGCCG ACGTGCCCAG TGACACTTCC AAAAATGACA AGAAAGGAAA GGCCAACACC
GCCAAAGCAA ATGTGACCCC TCAGAGTAGC TCTGAGCTCA GACCAACCAC CACGGCTGCC
CTGGCCTCTG GTGTGGAAGC CAAGAAAGGT ATTGAACTTA TCCCTCAAGT AAGGATAGAA
GACTTAAAGC AGATGAAGGC TTACTTGAAG CATTTAAAGA AACAGCAGAA GGAGCTAAAT
TCTTTAAAGA AGAAACATGC AAAGGAACAC AGTACCATGC AGAAGTTACA CTGCACGCAA
GTTGACAAAA TTGTGGCACA GTATGACAAA GAGAAGTCGA CTCATGAGAA AATCCTAGAG
AAGGCAATGA AGAAGAAGGG GGGAAGTAAT TGTCTCGAAA TGAAAAAAGA AACAGAAATC
AAAATTCAGA CGCTGACATC AGATCACAAA TCTAAGGTCA AAGAGATTGT AGCACAGCAC
ACAAAGGAAT GGTCAGAAAT GATCAATACC CACAGTGCTG AGGAGCAAGA AATCCGAGAC
CTGCACCTCA GCCAGCAGTG TGAGCTGCTG AAAAAGCTAC TCATCAATGC CCACGAGCAG
CAAACCCAGC AGCTGAAACT GTCCCATGAC AGGGAAAGCA AGGAAATGCG AGCACACCAG
GCTAAGATTT CTATGGAAAA TAGCAAAGCC ATCAGCCAAG ATAAATCTAT CAAGAATAAA
GCAGAACGGG AAAGGCGAGT CAGGGAGTTA AACAGCAGCA ACACTAAAAA GTTTCTGGAA
GAAAGAAAGA GACTTGCCAT GAAGCAGTCC AAAGAAATGG ATCAGTTGAA AAAAGTCCAG
CTTGAACATC TAGAATTCCT AGAGAAACAG AATGAGCAGC TTTTGAAATC CTGTCATGCA
GTGTCCCAAA CGCAAGGCGA AGGAGATGCA GCAGATGGTG AAATTGGAAG CCGAGATGGA
CCGCAGACCA GCAACAGTAG TATGAAACTC CAAAATGCAA ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14162
Clone ID Related Accession (Same CDS sequence) XM_017027881.1 , NM_182797.2
Accession Version NM_182797.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3528bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-08
Organism Homo sapiens(Human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB270238.1, BC117458.1, DR761451.1 and AL117633.1. On or before Aug 31, 2013 this sequence version replaced XM_005260727.1, NM_182797.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 3. The complete exon combination of the 5' UTR for this variant has not been determined. The resulting isoform (b) lacks an internal segment, compared to isoform c. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.222164.1, SRR1803617.16265.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGGCCTGGTC
ACTGTAGAAG ATGAGCAGGC GTGGATGGCA TCTTATAAAT ATGTAGGTGC TACCACTAAT
ATCCATCCAT ATTTGTCCAC AATGATCAAC TACGCCCAGC CTGTAAAGTT TCAAGGTTTC
CATGTGGCAG AAGAACGCAA TATTCATTAT AACATGTCTT CTTTTAATGA ATCAGTCGGT
CTTGGCTACT TGAAGACACA TGCAATTGAA TTTGTCAATT ATAACAAACG GCAAATGAGT
CGCATTTACC CCAAGGGAGG CCGAGTCGAT TCCAGTAATT ACATGCCTCA GATTTTCTGG
AACGCTGGCT GCCAGATGGT TTCACTGAAC TATCAAACCC CAGATTTAGC GATGCAATTG
AATCAGGGAA AATTTGAGTA TAATGGATCG TGCGGGTACC TTCTCAAACC AGATTTCATG
AGGCGGCCTG ATCGAACATT TGACCCCTTC TCTGAAACTC CTGTTGATGG TGTTATTGCA
GCCACTTGCT CAGTGCAGGT TATATCAGGT CAATTCTTAT CAGATAAGAA AATTGGCACC
TACGTAGAGG TGGATATGTA TGGGTTGCCC ACTGACACCA TACGTAAGGA ATTCCGAACT
CGCATGGTTA TGAATAATGG ACTCAATCCA GTTTACAATG AAGAGTCATT TGTATTTCGG
AAGGTGATCC TGCCGGACCT GGCTGTCTTG AGAATAGCTG TGTATGATGA TAACAACAAG
CTGATTGGCC AGAGGATCCT CCCGCTTGAT GGCCTCCAAG CCGGATATCG ACACATTTCC
CTTCGAAATG AGGGAAATAA ACCATTATCA CTACCAACAA TTTTCTGCAA TATTGTTCTT
AAAACATATG TGCCTGATGG ATTTGGAGAT ATCGTGGATG CTTTATCAGA TCCAAAGAAA
TTTCTCTCAA TTACAGAAAA GAGAGCAGAC CAAATGAGAG CTATGGGCAT TGAAACTAGT
GACATAGCCG ACGTGCCCAG TGACACTTCC AAAAATGACA AGAAAGGAAA GGCCAACACC
GCCAAAGCAA ATGTGACCCC TCAGAGTAGC TCTGAGCTCA GACCAACCAC CACGGCTGCC
CTGGCCTCTG GTGTGGAAGC CAAGAAAGGT ATTGAACTTA TCCCTCAAGT AAGGATAGAA
GACTTAAAGC AGATGAAGGC TTACTTGAAG CATTTAAAGA AACAGCAGAA GGAGCTAAAT
TCTTTAAAGA AGAAACATGC AAAGGAACAC AGTACCATGC AGAAGTTACA CTGCACGCAA
GTTGACAAAA TTGTGGCACA GTATGACAAA GAGAAGTCGA CTCATGAGAA AATCCTAGAG
AAGGCAATGA AGAAGAAGGG GGGAAGTAAT TGTCTCGAAA TGAAAAAAGA AACAGAAATC
AAAATTCAGA CGCTGACATC AGATCACAAA TCTAAGGTCA AAGAGATTGT AGCACAGCAC
ACAAAGGAAT GGTCAGAAAT GATCAATACC CACAGTGCTG AGGAGCAAGA AATCCGAGAC
CTGCACCTCA GCCAGCAGTG TGAGCTGCTG AAAAAGCTAC TCATCAATGC CCACGAGCAG
CAAACCCAGC AGCTGAAACT GTCCCATGAC AGGGAAAGCA AGGAAATGCG AGCACACCAG
GCTAAGATTT CTATGGAAAA TAGCAAAGCC ATCAGCCAAG ATAAATCTAT CAAGAATAAA
GCAGAACGGG AAAGGCGAGT CAGGGAGTTA AACAGCAGCA ACACTAAAAA GTTTCTGGAA
GAAAGAAAGA GACTTGCCAT GAAGCAGTCC AAAGAAATGG ATCAGTTGAA AAAAGTCCAG
CTTGAACATC TAGAATTCCT AGAGAAACAG AATGAGCAGG CGAAGGAGAT GCAGCAGATG
GTGAAATTGG AAGCCGAGAT GGACCGCAGA CCAGCAACAG TAGTATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_877949.2
CDS79..3606
Misc Feature(1)22..24
Misc Feature(2)82..84
Misc Feature(3)127..507
Misc Feature(4)order(142..144,148..150,184..186,235..237,427..429)
Misc Feature(5)order(151..153,157..159,175..177,181..183,187..189,232..237)
Misc Feature(6)730..2499
Misc Feature(7)739..1014
Misc Feature(8)1012..2082
Misc Feature(9)order(1060..1065,1150..1152,1156..1158,1201..1203,1303..1305,1459..1461,1465..1467,1858..1860,1939..1941,1945..1947)
Misc Feature(10)order(1060..1062,1201..1203)
Misc Feature(11)order(1063..1065,1150..1152,1156..1158,1303..1305)
Misc Feature(12)2182..2532
Misc Feature(13)order(2233..2235,2311..2313,2404..2406,2410..2412,2416..2418,2422..2424)
Misc Feature(14)2734..2736
Misc Feature(15)2818..2940
Misc Feature(16)3220..3558
Exon (1)1..63
Exon (2)64..162
Exon (3)163..243
Exon (4)244..303
Exon (5)304..447
Exon (6)448..527
Exon (7)528..581
Exon (8)582..663
Exon (9)664..764
Exon (10)765..822
Exon (11)823..931
Exon (12)932..1142
Exon (13)1143..1236
Exon (14)1237..1316
Exon (15)1317..1401
Exon (16)1402..1492
Exon (17)1493..1588
Exon (18)1589..1689
Exon (19)1690..1831
Exon (20)1832..1916
Exon (21)1917..2041
Exon (22)2042..2093
Exon (23)2094..2196
Exon (24)2197..2361
Exon (25)2362..2566
Exon (26)2567..2655
Exon (27)2656..2806
Exon (28)2807..2856
Exon (29)2857..2922
Exon (30)2923..3038
Exon (31)3039..3114
Exon (32)3115..3290
Exon (33)3291..3392
Exon (34)3393..3450
Exon (35)3451..3537
Exon (36)3538..5433
Translation

Target ORF information:

RefSeq Version NM_182797.2
Organism Homo sapiens(Human)
Definition Homo sapiens phospholipase C beta 4 (PLCB4), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_182797.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGGCCTGGTC
ACTGTAGAAG ATGAGCAGGC GTGGATGGCA TCTTATAAAT ATGTAGGTGC TACCACTAAT
ATCCATCCAT ATTTGTCCAC AATGATCAAC TACGCCCAGC CTGTAAAGTT TCAAGGTTTC
CATGTGGCAG AAGAACGCAA TATTCATTAT AACATGTCTT CTTTTAATGA ATCAGTCGGT
CTTGGCTACT TGAAGACACA TGCAATTGAA TTTGTCAATT ATAACAAACG GCAAATGAGT
CGCATTTACC CCAAGGGAGG CCGAGTCGAT TCCAGTAATT ACATGCCTCA GATTTTCTGG
AACGCTGGCT GCCAGATGGT TTCACTGAAC TATCAAACCC CAGATTTAGC GATGCAATTG
AATCAGGGAA AATTTGAGTA TAATGGATCG TGCGGGTACC TTCTCAAACC AGATTTCATG
AGGCGGCCTG ATCGAACATT TGACCCCTTC TCTGAAACTC CTGTTGATGG TGTTATTGCA
GCCACTTGCT CAGTGCAGGT TATATCAGGT CAATTCTTAT CAGATAAGAA AATTGGCACC
TACGTAGAGG TGGATATGTA TGGGTTGCCC ACTGACACCA TACGTAAGGA ATTCCGAACT
CGCATGGTTA TGAATAATGG ACTCAATCCA GTTTACAATG AAGAGTCATT TGTATTTCGG
AAGGTGATCC TGCCGGACCT GGCTGTCTTG AGAATAGCTG TGTATGATGA TAACAACAAG
CTGATTGGCC AGAGGATCCT CCCGCTTGAT GGCCTCCAAG CCGGATATCG ACACATTTCC
CTTCGAAATG AGGGAAATAA ACCATTATCA CTACCAACAA TTTTCTGCAA TATTGTTCTT
AAAACATATG TGCCTGATGG ATTTGGAGAT ATCGTGGATG CTTTATCAGA TCCAAAGAAA
TTTCTCTCAA TTACAGAAAA GAGAGCAGAC CAAATGAGAG CTATGGGCAT TGAAACTAGT
GACATAGCCG ACGTGCCCAG TGACACTTCC AAAAATGACA AGAAAGGAAA GGCCAACACC
GCCAAAGCAA ATGTGACCCC TCAGAGTAGC TCTGAGCTCA GACCAACCAC CACGGCTGCC
CTGGCCTCTG GTGTGGAAGC CAAGAAAGGT ATTGAACTTA TCCCTCAAGT AAGGATAGAA
GACTTAAAGC AGATGAAGGC TTACTTGAAG CATTTAAAGA AACAGCAGAA GGAGCTAAAT
TCTTTAAAGA AGAAACATGC AAAGGAACAC AGTACCATGC AGAAGTTACA CTGCACGCAA
GTTGACAAAA TTGTGGCACA GTATGACAAA GAGAAGTCGA CTCATGAGAA AATCCTAGAG
AAGGCAATGA AGAAGAAGGG GGGAAGTAAT TGTCTCGAAA TGAAAAAAGA AACAGAAATC
AAAATTCAGA CGCTGACATC AGATCACAAA TCTAAGGTCA AAGAGATTGT AGCACAGCAC
ACAAAGGAAT GGTCAGAAAT GATCAATACC CACAGTGCTG AGGAGCAAGA AATCCGAGAC
CTGCACCTCA GCCAGCAGTG TGAGCTGCTG AAAAAGCTAC TCATCAATGC CCACGAGCAG
CAAACCCAGC AGCTGAAACT GTCCCATGAC AGGGAAAGCA AGGAAATGCG AGCACACCAG
GCTAAGATTT CTATGGAAAA TAGCAAAGCC ATCAGCCAAG ATAAATCTAT CAAGAATAAA
GCAGAACGGG AAAGGCGAGT CAGGGAGTTA AACAGCAGCA ACACTAAAAA GTTTCTGGAA
GAAAGAAAGA GACTTGCCAT GAAGCAGTCC AAAGAAATGG ATCAGTTGAA AAAAGTCCAG
CTTGAACATC TAGAATTCCT AGAGAAACAG AATGAGCAGG CGAAGGAGAT GCAGCAGATG
GTGAAATTGG AAGCCGAGAT GGACCGCAGA CCAGCAACAG TAGTATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18159
Clone ID Related Accession (Same CDS sequence) NM_001172646.1
Accession Version NM_001172646.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3564bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-17
Organism Homo sapiens(Human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform c
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK307837.1, DB270238.1, BC143868.1, DN913865.1 and AL117633.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (3) represents the longest transcript and encodes isoform c. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2145240 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGCTTCAGAT
GACCTTGAAC ATGAAAACAA CAAAAAGGGC CTGGTCACTG TAGAAGATGA GCAGGCGTGG
ATGGCATCTT ATAAATATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG
ATCAACTACG CCCAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCAGAAGA ACGCAATATT
CATTATAACA TGTCTTCTTT TAATGAATCA GTCGGTCTTG GCTACTTGAA GACACATGCA
ATTGAATTTG TCAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGA
GTCGATTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCA
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
GGATCGTGCG GGTACCTTCT CAAACCAGAT TTCATGAGGC GGCCTGATCG AACATTTGAC
CCCTTCTCTG AAACTCCTGT TGATGGTGTT ATTGCAGCCA CTTGCTCAGT GCAGGTTATA
TCAGGTCAAT TCTTATCAGA TAAGAAAATT GGCACCTACG TAGAGGTGGA TATGTATGGG
TTGCCCACTG ACACCATACG TAAGGAATTC CGAACTCGCA TGGTTATGAA TAATGGACTC
AATCCAGTTT ACAATGAAGA GTCATTTGTA TTTCGGAAGG TGATCCTGCC GGACCTGGCT
GTCTTGAGAA TAGCTGTGTA TGATGATAAC AACAAGCTGA TTGGCCAGAG GATCCTCCCG
CTTGATGGCC TCCAAGCCGG ATATCGACAC ATTTCCCTTC GAAATGAGGG AAATAAACCA
TTATCACTAC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
GGAGATATCG TGGATGCTTT ATCAGATCCA AAGAAATTTC TCTCAATTAC AGAAAAGAGA
GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCCGACGT GCCCAGTGAC
ACTTCCAAAA ATGACAAGAA AGGAAAGGCC AACACCGCCA AAGCAAATGT GACCCCTCAG
AGTAGCTCTG AGCTCAGACC AACCACCACG GCTGCCCTGG CCTCTGGTGT GGAAGCCAAG
AAAGGTATTG AACTTATCCC TCAAGTAAGG ATAGAAGACT TAAAGCAGAT GAAGGCTTAC
TTGAAGCATT TAAAGAAACA GCAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCAAAG
GAACACAGTA CCATGCAGAA GTTACACTGC ACGCAAGTTG ACAAAATTGT GGCACAGTAT
GACAAAGAGA AGTCGACTCA TGAGAAAATC CTAGAGAAGG CAATGAAGAA GAAGGGGGGA
AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATCAAAA TTCAGACGCT GACATCAGAT
CACAAATCTA AGGTCAAAGA GATTGTAGCA CAGCACACAA AGGAATGGTC AGAAATGATC
AATACCCACA GTGCTGAGGA GCAAGAAATC CGAGACCTGC ACCTCAGCCA GCAGTGTGAG
CTGCTGAAAA AGCTACTCAT CAATGCCCAC GAGCAGCAAA CCCAGCAGCT GAAACTGTCC
CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAATAGC
AAAGCCATCA GCCAAGATAA ATCTATCAAG AATAAAGCAG AACGGGAAAG GCGAGTCAGG
GAGTTAAACA GCAGCAACAC TAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCCATGAAG
CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACATCTAGA ATTCCTAGAG
AAACAGAATG AGCAGGCGAA GGAGATGCAG CAGATGGTGA AATTGGAAGC CGAGATGGAC
CGCAGACCAG CAACAGTAGT ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001166117.1
CDS232..3795
Misc Feature(1)175..177
Misc Feature(2)235..237
Misc Feature(3)280..660
Misc Feature(4)order(295..297,301..303,337..339,388..390,580..582)
Misc Feature(5)order(304..306,310..312,328..330,334..336,340..342,385..390)
Misc Feature(6)883..2688
Misc Feature(7)892..1167
Misc Feature(8)1165..2271
Misc Feature(9)order(1213..1218,1303..1305,1309..1311,1354..1356,1456..1458,1612..1614,1618..1620,2047..2049,2128..2130,2134..2136)
Misc Feature(10)order(1213..1215,1354..1356)
Misc Feature(11)order(1216..1218,1303..1305,1309..1311,1456..1458)
Misc Feature(12)2371..2721
Misc Feature(13)order(2422..2424,2500..2502,2593..2595,2599..2601,2605..2607,2611..2613)
Misc Feature(14)2923..2925
Misc Feature(15)3007..3129
Misc Feature(16)3409..3747
Exon (1)1..153
Exon (2)154..216
Exon (3)217..315
Exon (4)316..396
Exon (5)397..456
Exon (6)457..600
Exon (7)601..680
Exon (8)681..734
Exon (9)735..816
Exon (10)817..917
Exon (11)918..975
Exon (12)976..1084
Exon (13)1085..1295
Exon (14)1296..1389
Exon (15)1390..1469
Exon (16)1470..1554
Exon (17)1555..1645
Exon (18)1646..1741
Exon (19)1742..1842
Exon (20)1843..1878
Exon (21)1879..2020
Exon (22)2021..2105
Exon (23)2106..2230
Exon (24)2231..2282
Exon (25)2283..2385
Exon (26)2386..2550
Exon (27)2551..2755
Exon (28)2756..2844
Exon (29)2845..2995
Exon (30)2996..3045
Exon (31)3046..3111
Exon (32)3112..3227
Exon (33)3228..3303
Exon (34)3304..3479
Exon (35)3480..3581
Exon (36)3582..3639
Exon (37)3640..3726
Exon (38)3727..5622
Translation

Target ORF information:

RefSeq Version NM_001172646.1
Organism Homo sapiens(Human)
Definition Homo sapiens phospholipase C beta 4 (PLCB4), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001172646.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGCTTCAGAT
GACCTTGAAC ATGAAAACAA CAAAAAGGGC CTGGTCACTG TAGAAGATGA GCAGGCGTGG
ATGGCATCTT ATAAATATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG
ATCAACTACG CCCAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCAGAAGA ACGCAATATT
CATTATAACA TGTCTTCTTT TAATGAATCA GTCGGTCTTG GCTACTTGAA GACACATGCA
ATTGAATTTG TCAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGA
GTCGATTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCA
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
GGATCGTGCG GGTACCTTCT CAAACCAGAT TTCATGAGGC GGCCTGATCG AACATTTGAC
CCCTTCTCTG AAACTCCTGT TGATGGTGTT ATTGCAGCCA CTTGCTCAGT GCAGGTTATA
TCAGGTCAAT TCTTATCAGA TAAGAAAATT GGCACCTACG TAGAGGTGGA TATGTATGGG
TTGCCCACTG ACACCATACG TAAGGAATTC CGAACTCGCA TGGTTATGAA TAATGGACTC
AATCCAGTTT ACAATGAAGA GTCATTTGTA TTTCGGAAGG TGATCCTGCC GGACCTGGCT
GTCTTGAGAA TAGCTGTGTA TGATGATAAC AACAAGCTGA TTGGCCAGAG GATCCTCCCG
CTTGATGGCC TCCAAGCCGG ATATCGACAC ATTTCCCTTC GAAATGAGGG AAATAAACCA
TTATCACTAC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
GGAGATATCG TGGATGCTTT ATCAGATCCA AAGAAATTTC TCTCAATTAC AGAAAAGAGA
GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCCGACGT GCCCAGTGAC
ACTTCCAAAA ATGACAAGAA AGGAAAGGCC AACACCGCCA AAGCAAATGT GACCCCTCAG
AGTAGCTCTG AGCTCAGACC AACCACCACG GCTGCCCTGG CCTCTGGTGT GGAAGCCAAG
AAAGGTATTG AACTTATCCC TCAAGTAAGG ATAGAAGACT TAAAGCAGAT GAAGGCTTAC
TTGAAGCATT TAAAGAAACA GCAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCAAAG
GAACACAGTA CCATGCAGAA GTTACACTGC ACGCAAGTTG ACAAAATTGT GGCACAGTAT
GACAAAGAGA AGTCGACTCA TGAGAAAATC CTAGAGAAGG CAATGAAGAA GAAGGGGGGA
AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATCAAAA TTCAGACGCT GACATCAGAT
CACAAATCTA AGGTCAAAGA GATTGTAGCA CAGCACACAA AGGAATGGTC AGAAATGATC
AATACCCACA GTGCTGAGGA GCAAGAAATC CGAGACCTGC ACCTCAGCCA GCAGTGTGAG
CTGCTGAAAA AGCTACTCAT CAATGCCCAC GAGCAGCAAA CCCAGCAGCT GAAACTGTCC
CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAATAGC
AAAGCCATCA GCCAAGATAA ATCTATCAAG AATAAAGCAG AACGGGAAAG GCGAGTCAGG
GAGTTAAACA GCAGCAACAC TAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCCATGAAG
CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACATCTAGA ATTCCTAGAG
AAACAGAATG AGCAGGCGAA GGAGATGCAG CAGATGGTGA AATTGGAAGC CGAGATGGAC
CGCAGACCAG CAACAGTAGT ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14145
Clone ID Related Accession (Same CDS sequence) NM_000933.3 , XM_017027880.1
Accession Version XM_017027880.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3585bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGGCCTGGTC
ACTGTAGAAG ATGAGCAGGC GTGGATGGCA TCTTATAAAT ATGTAGGTGC TACCACTAAT
ATCCATCCAT ATTTGTCCAC AATGATCAAC TACGCCCAGC CTGTAAAGTT TCAAGGTTTC
CATGTGGCAG AAGAACGCAA TATTCATTAT AACATGTCTT CTTTTAATGA ATCAGTCGGT
CTTGGCTACT TGAAGACACA TGCAATTGAA TTTGTCAATT ATAACAAACG GCAAATGAGT
CGCATTTACC CCAAGGGAGG CCGAGTCGAT TCCAGTAATT ACATGCCTCA GATTTTCTGG
AACGCTGGCT GCCAGATGGT TTCACTGAAC TATCAAACCC CAGATTTAGC GATGCAATTG
AATCAGGGAA AATTTGAGTA TAATGGATCG TGCGGGTACC TTCTCAAACC AGATTTCATG
AGGCGGCCTG ATCGAACATT TGACCCCTTC TCTGAAACTC CTGTTGATGG TGTTATTGCA
GCCACTTGCT CAGTGCAGGT TATATCAGGT CAATTCTTAT CAGATAAGAA AATTGGCACC
TACGTAGAGG TGGATATGTA TGGGTTGCCC ACTGACACCA TACGTAAGGA ATTCCGAACT
CGCATGGTTA TGAATAATGG ACTCAATCCA GTTTACAATG AAGAGTCATT TGTATTTCGG
AAGGTGATCC TGCCGGACCT GGCTGTCTTG AGAATAGCTG TGTATGATGA TAACAACAAG
CTGATTGGCC AGAGGATCCT CCCGCTTGAT GGCCTCCAAG CCGGATATCG ACACATTTCC
CTTCGAAATG AGGGAAATAA ACCATTATCA CTACCAACAA TTTTCTGCAA TATTGTTCTT
AAAACATATG TGCCTGATGG ATTTGGAGAT ATCGTGGATG CTTTATCAGA TCCAAAGAAA
TTTCTCTCAA TTACAGAAAA GAGAGCAGAC CAAATGAGAG CTATGGGCAT TGAAACTAGT
GACATAGCCG ACGTGCCCAG TGACACTTCC AAAAATGACA AGAAAGGAAA GGCCAACACC
GCCAAAGCAA ATGTGACCCC TCAGAGTAGC TCTGAGCTCA GACCAACCAC CACGGCTGCC
CTGGCCTCTG GTGTGGAAGC CAAGAAAGGT ATTGAACTTA TCCCTCAAGT AAGGATAGAA
GACTTAAAGC AGATGAAGGC TTACTTGAAG CATTTAAAGA AACAGCAGAA GGAGCTAAAT
TCTTTAAAGA AGAAACATGC AAAGGAACAC AGTACCATGC AGAAGTTACA CTGCACGCAA
GTTGACAAAA TTGTGGCACA GTATGACAAA GAGAAGTCGA CTCATGAGAA AATCCTAGAG
AAGGCAATGA AGAAGAAGGG GGGAAGTAAT TGTCTCGAAA TGAAAAAAGA AACAGAAATC
AAAATTCAGA CGCTGACATC AGATCACAAA TCTAAGGTCA AAGAGATTGT AGCACAGCAC
ACAAAGGAAT GGTCAGAAAT GATCAATACC CACAGTGCTG AGGAGCAAGA AATCCGAGAC
CTGCACCTCA GCCAGCAGTG TGAGCTGCTG AAAAAGCTAC TCATCAATGC CCACGAGCAG
CAAACCCAGC AGCTGAAACT GTCCCATGAC AGGGAAAGCA AGGAAATGCG AGCACACCAG
GCTAAGATTT CTATGGAAAA TAGCAAAGCC ATCAGCCAAG ATAAATCTAT CAAGAATAAA
GCAGAACGGG AAAGGCGAGT CAGGGAGTTA AACAGCAGCA ACACTAAAAA GTTTCTGGAA
GAAAGAAAGA GACTTGCCAT GAAGCAGTCC AAAGAAATGG ATCAGTTGAA AAAAGTCCAG
CTTGAACATC TAGAATTCCT AGAGAAACAG AATGAGCAGC TTTTGAAATC CTGTCATGCA
GTGTCCCAAA CGCAAGGCGA AGGAGATGCA GCAGATGGTG AAATTGGAAG CCGAGATGGA
CCGCAGACCA GCAACAGTAG TATGAAACTC CAAAATGCAA ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016883369.1
CDS273..3857
Misc Feature(1)321..701
Misc Feature(2)order(336..338,342..344,378..380,429..431,621..623)
Misc Feature(3)order(345..347,351..353,369..371,375..377,381..383,426..431)
Misc Feature(4)924..2693
Misc Feature(5)933..1208
Misc Feature(6)1206..2276
Misc Feature(7)order(1254..1259,1344..1346,1350..1352,1395..1397,1497..1499,1653..1655,1659..1661,2052..2054,2133..2135,2139..2141)
Misc Feature(8)order(1254..1256,1395..1397)
Misc Feature(9)order(1257..1259,1344..1346,1350..1352,1497..1499)
Misc Feature(10)2376..2726
Misc Feature(11)order(2427..2429,2505..2507,2598..2600,2604..2606,2610..2612,2616..2618)
Misc Feature(12)3012..3134
Misc Feature(13)3057..3770
Translation

Target ORF information:

RefSeq Version XM_017027880.1
Organism Homo sapiens(Human)
Definition Homo sapiens phospholipase C beta 4 (PLCB4), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017027880.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGGCCTGGTC
ACTGTAGAAG ATGAGCAGGC GTGGATGGCA TCTTATAAAT ATGTAGGTGC TACCACTAAT
ATCCATCCAT ATTTGTCCAC AATGATCAAC TACGCCCAGC CTGTAAAGTT TCAAGGTTTC
CATGTGGCAG AAGAACGCAA TATTCATTAT AACATGTCTT CTTTTAATGA ATCAGTCGGT
CTTGGCTACT TGAAGACACA TGCAATTGAA TTTGTCAATT ATAACAAACG GCAAATGAGT
CGCATTTACC CCAAGGGAGG CCGAGTCGAT TCCAGTAATT ACATGCCTCA GATTTTCTGG
AACGCTGGCT GCCAGATGGT TTCACTGAAC TATCAAACCC CAGATTTAGC GATGCAATTG
AATCAGGGAA AATTTGAGTA TAATGGATCG TGCGGGTACC TTCTCAAACC AGATTTCATG
AGGCGGCCTG ATCGAACATT TGACCCCTTC TCTGAAACTC CTGTTGATGG TGTTATTGCA
GCCACTTGCT CAGTGCAGGT TATATCAGGT CAATTCTTAT CAGATAAGAA AATTGGCACC
TACGTAGAGG TGGATATGTA TGGGTTGCCC ACTGACACCA TACGTAAGGA ATTCCGAACT
CGCATGGTTA TGAATAATGG ACTCAATCCA GTTTACAATG AAGAGTCATT TGTATTTCGG
AAGGTGATCC TGCCGGACCT GGCTGTCTTG AGAATAGCTG TGTATGATGA TAACAACAAG
CTGATTGGCC AGAGGATCCT CCCGCTTGAT GGCCTCCAAG CCGGATATCG ACACATTTCC
CTTCGAAATG AGGGAAATAA ACCATTATCA CTACCAACAA TTTTCTGCAA TATTGTTCTT
AAAACATATG TGCCTGATGG ATTTGGAGAT ATCGTGGATG CTTTATCAGA TCCAAAGAAA
TTTCTCTCAA TTACAGAAAA GAGAGCAGAC CAAATGAGAG CTATGGGCAT TGAAACTAGT
GACATAGCCG ACGTGCCCAG TGACACTTCC AAAAATGACA AGAAAGGAAA GGCCAACACC
GCCAAAGCAA ATGTGACCCC TCAGAGTAGC TCTGAGCTCA GACCAACCAC CACGGCTGCC
CTGGCCTCTG GTGTGGAAGC CAAGAAAGGT ATTGAACTTA TCCCTCAAGT AAGGATAGAA
GACTTAAAGC AGATGAAGGC TTACTTGAAG CATTTAAAGA AACAGCAGAA GGAGCTAAAT
TCTTTAAAGA AGAAACATGC AAAGGAACAC AGTACCATGC AGAAGTTACA CTGCACGCAA
GTTGACAAAA TTGTGGCACA GTATGACAAA GAGAAGTCGA CTCATGAGAA AATCCTAGAG
AAGGCAATGA AGAAGAAGGG GGGAAGTAAT TGTCTCGAAA TGAAAAAAGA AACAGAAATC
AAAATTCAGA CGCTGACATC AGATCACAAA TCTAAGGTCA AAGAGATTGT AGCACAGCAC
ACAAAGGAAT GGTCAGAAAT GATCAATACC CACAGTGCTG AGGAGCAAGA AATCCGAGAC
CTGCACCTCA GCCAGCAGTG TGAGCTGCTG AAAAAGCTAC TCATCAATGC CCACGAGCAG
CAAACCCAGC AGCTGAAACT GTCCCATGAC AGGGAAAGCA AGGAAATGCG AGCACACCAG
GCTAAGATTT CTATGGAAAA TAGCAAAGCC ATCAGCCAAG ATAAATCTAT CAAGAATAAA
GCAGAACGGG AAAGGCGAGT CAGGGAGTTA AACAGCAGCA ACACTAAAAA GTTTCTGGAA
GAAAGAAAGA GACTTGCCAT GAAGCAGTCC AAAGAAATGG ATCAGTTGAA AAAAGTCCAG
CTTGAACATC TAGAATTCCT AGAGAAACAG AATGAGCAGC TTTTGAAATC CTGTCATGCA
GTGTCCCAAA CGCAAGGCGA AGGAGATGCA GCAGATGGTG AAATTGGAAG CCGAGATGGA
CCGCAGACCA GCAACAGTAG TATGAAACTC CAAAATGCAA ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14162
Clone ID Related Accession (Same CDS sequence) XM_017027881.1 , NM_182797.2
Accession Version XM_017027881.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3528bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGGCCTGGTC
ACTGTAGAAG ATGAGCAGGC GTGGATGGCA TCTTATAAAT ATGTAGGTGC TACCACTAAT
ATCCATCCAT ATTTGTCCAC AATGATCAAC TACGCCCAGC CTGTAAAGTT TCAAGGTTTC
CATGTGGCAG AAGAACGCAA TATTCATTAT AACATGTCTT CTTTTAATGA ATCAGTCGGT
CTTGGCTACT TGAAGACACA TGCAATTGAA TTTGTCAATT ATAACAAACG GCAAATGAGT
CGCATTTACC CCAAGGGAGG CCGAGTCGAT TCCAGTAATT ACATGCCTCA GATTTTCTGG
AACGCTGGCT GCCAGATGGT TTCACTGAAC TATCAAACCC CAGATTTAGC GATGCAATTG
AATCAGGGAA AATTTGAGTA TAATGGATCG TGCGGGTACC TTCTCAAACC AGATTTCATG
AGGCGGCCTG ATCGAACATT TGACCCCTTC TCTGAAACTC CTGTTGATGG TGTTATTGCA
GCCACTTGCT CAGTGCAGGT TATATCAGGT CAATTCTTAT CAGATAAGAA AATTGGCACC
TACGTAGAGG TGGATATGTA TGGGTTGCCC ACTGACACCA TACGTAAGGA ATTCCGAACT
CGCATGGTTA TGAATAATGG ACTCAATCCA GTTTACAATG AAGAGTCATT TGTATTTCGG
AAGGTGATCC TGCCGGACCT GGCTGTCTTG AGAATAGCTG TGTATGATGA TAACAACAAG
CTGATTGGCC AGAGGATCCT CCCGCTTGAT GGCCTCCAAG CCGGATATCG ACACATTTCC
CTTCGAAATG AGGGAAATAA ACCATTATCA CTACCAACAA TTTTCTGCAA TATTGTTCTT
AAAACATATG TGCCTGATGG ATTTGGAGAT ATCGTGGATG CTTTATCAGA TCCAAAGAAA
TTTCTCTCAA TTACAGAAAA GAGAGCAGAC CAAATGAGAG CTATGGGCAT TGAAACTAGT
GACATAGCCG ACGTGCCCAG TGACACTTCC AAAAATGACA AGAAAGGAAA GGCCAACACC
GCCAAAGCAA ATGTGACCCC TCAGAGTAGC TCTGAGCTCA GACCAACCAC CACGGCTGCC
CTGGCCTCTG GTGTGGAAGC CAAGAAAGGT ATTGAACTTA TCCCTCAAGT AAGGATAGAA
GACTTAAAGC AGATGAAGGC TTACTTGAAG CATTTAAAGA AACAGCAGAA GGAGCTAAAT
TCTTTAAAGA AGAAACATGC AAAGGAACAC AGTACCATGC AGAAGTTACA CTGCACGCAA
GTTGACAAAA TTGTGGCACA GTATGACAAA GAGAAGTCGA CTCATGAGAA AATCCTAGAG
AAGGCAATGA AGAAGAAGGG GGGAAGTAAT TGTCTCGAAA TGAAAAAAGA AACAGAAATC
AAAATTCAGA CGCTGACATC AGATCACAAA TCTAAGGTCA AAGAGATTGT AGCACAGCAC
ACAAAGGAAT GGTCAGAAAT GATCAATACC CACAGTGCTG AGGAGCAAGA AATCCGAGAC
CTGCACCTCA GCCAGCAGTG TGAGCTGCTG AAAAAGCTAC TCATCAATGC CCACGAGCAG
CAAACCCAGC AGCTGAAACT GTCCCATGAC AGGGAAAGCA AGGAAATGCG AGCACACCAG
GCTAAGATTT CTATGGAAAA TAGCAAAGCC ATCAGCCAAG ATAAATCTAT CAAGAATAAA
GCAGAACGGG AAAGGCGAGT CAGGGAGTTA AACAGCAGCA ACACTAAAAA GTTTCTGGAA
GAAAGAAAGA GACTTGCCAT GAAGCAGTCC AAAGAAATGG ATCAGTTGAA AAAAGTCCAG
CTTGAACATC TAGAATTCCT AGAGAAACAG AATGAGCAGG CGAAGGAGAT GCAGCAGATG
GTGAAATTGG AAGCCGAGAT GGACCGCAGA CCAGCAACAG TAGTATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016883370.1
CDS255..3782
Misc Feature(1)303..683
Misc Feature(2)order(318..320,324..326,360..362,411..413,603..605)
Misc Feature(3)order(327..329,333..335,351..353,357..359,363..365,408..413)
Misc Feature(4)906..2675
Misc Feature(5)915..1190
Misc Feature(6)1188..2258
Misc Feature(7)order(1236..1241,1326..1328,1332..1334,1377..1379,1479..1481,1635..1637,1641..1643,2034..2036,2115..2117,2121..2123)
Misc Feature(8)order(1236..1238,1377..1379)
Misc Feature(9)order(1239..1241,1326..1328,1332..1334,1479..1481)
Misc Feature(10)2358..2708
Misc Feature(11)order(2409..2411,2487..2489,2580..2582,2586..2588,2592..2594,2598..2600)
Misc Feature(12)2994..3116
Misc Feature(13)3396..3734
Translation

Target ORF information:

RefSeq Version XM_017027881.1
Organism Homo sapiens(Human)
Definition Homo sapiens phospholipase C beta 4 (PLCB4), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017027881.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGGCCTGGTC
ACTGTAGAAG ATGAGCAGGC GTGGATGGCA TCTTATAAAT ATGTAGGTGC TACCACTAAT
ATCCATCCAT ATTTGTCCAC AATGATCAAC TACGCCCAGC CTGTAAAGTT TCAAGGTTTC
CATGTGGCAG AAGAACGCAA TATTCATTAT AACATGTCTT CTTTTAATGA ATCAGTCGGT
CTTGGCTACT TGAAGACACA TGCAATTGAA TTTGTCAATT ATAACAAACG GCAAATGAGT
CGCATTTACC CCAAGGGAGG CCGAGTCGAT TCCAGTAATT ACATGCCTCA GATTTTCTGG
AACGCTGGCT GCCAGATGGT TTCACTGAAC TATCAAACCC CAGATTTAGC GATGCAATTG
AATCAGGGAA AATTTGAGTA TAATGGATCG TGCGGGTACC TTCTCAAACC AGATTTCATG
AGGCGGCCTG ATCGAACATT TGACCCCTTC TCTGAAACTC CTGTTGATGG TGTTATTGCA
GCCACTTGCT CAGTGCAGGT TATATCAGGT CAATTCTTAT CAGATAAGAA AATTGGCACC
TACGTAGAGG TGGATATGTA TGGGTTGCCC ACTGACACCA TACGTAAGGA ATTCCGAACT
CGCATGGTTA TGAATAATGG ACTCAATCCA GTTTACAATG AAGAGTCATT TGTATTTCGG
AAGGTGATCC TGCCGGACCT GGCTGTCTTG AGAATAGCTG TGTATGATGA TAACAACAAG
CTGATTGGCC AGAGGATCCT CCCGCTTGAT GGCCTCCAAG CCGGATATCG ACACATTTCC
CTTCGAAATG AGGGAAATAA ACCATTATCA CTACCAACAA TTTTCTGCAA TATTGTTCTT
AAAACATATG TGCCTGATGG ATTTGGAGAT ATCGTGGATG CTTTATCAGA TCCAAAGAAA
TTTCTCTCAA TTACAGAAAA GAGAGCAGAC CAAATGAGAG CTATGGGCAT TGAAACTAGT
GACATAGCCG ACGTGCCCAG TGACACTTCC AAAAATGACA AGAAAGGAAA GGCCAACACC
GCCAAAGCAA ATGTGACCCC TCAGAGTAGC TCTGAGCTCA GACCAACCAC CACGGCTGCC
CTGGCCTCTG GTGTGGAAGC CAAGAAAGGT ATTGAACTTA TCCCTCAAGT AAGGATAGAA
GACTTAAAGC AGATGAAGGC TTACTTGAAG CATTTAAAGA AACAGCAGAA GGAGCTAAAT
TCTTTAAAGA AGAAACATGC AAAGGAACAC AGTACCATGC AGAAGTTACA CTGCACGCAA
GTTGACAAAA TTGTGGCACA GTATGACAAA GAGAAGTCGA CTCATGAGAA AATCCTAGAG
AAGGCAATGA AGAAGAAGGG GGGAAGTAAT TGTCTCGAAA TGAAAAAAGA AACAGAAATC
AAAATTCAGA CGCTGACATC AGATCACAAA TCTAAGGTCA AAGAGATTGT AGCACAGCAC
ACAAAGGAAT GGTCAGAAAT GATCAATACC CACAGTGCTG AGGAGCAAGA AATCCGAGAC
CTGCACCTCA GCCAGCAGTG TGAGCTGCTG AAAAAGCTAC TCATCAATGC CCACGAGCAG
CAAACCCAGC AGCTGAAACT GTCCCATGAC AGGGAAAGCA AGGAAATGCG AGCACACCAG
GCTAAGATTT CTATGGAAAA TAGCAAAGCC ATCAGCCAAG ATAAATCTAT CAAGAATAAA
GCAGAACGGG AAAGGCGAGT CAGGGAGTTA AACAGCAGCA ACACTAAAAA GTTTCTGGAA
GAAAGAAAGA GACTTGCCAT GAAGCAGTCC AAAGAAATGG ATCAGTTGAA AAAAGTCCAG
CTTGAACATC TAGAATTCCT AGAGAAACAG AATGAGCAGG CGAAGGAGAT GCAGCAGATG
GTGAAATTGG AAGCCGAGAT GGACCGCAGA CCAGCAACAG TAGTATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu52719
Clone ID Related Accession (Same CDS sequence) XM_006723569.2 , XM_005260724.2 , XM_006723568.3
Accession Version XM_006723569.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3621bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006723569.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGCTTCAGAT
GACCTTGAAC ATGAAAACAA CAAAAAGGGC CTGGTCACTG TAGAAGATGA GCAGGCGTGG
ATGGCATCTT ATAAATATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG
ATCAACTACG CCCAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCAGAAGA ACGCAATATT
CATTATAACA TGTCTTCTTT TAATGAATCA GTCGGTCTTG GCTACTTGAA GACACATGCA
ATTGAATTTG TCAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGA
GTCGATTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCA
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
GGATCGTGCG GGTACCTTCT CAAACCAGAT TTCATGAGGC GGCCTGATCG AACATTTGAC
CCCTTCTCTG AAACTCCTGT TGATGGTGTT ATTGCAGCCA CTTGCTCAGT GCAGGTTATA
TCAGGTCAAT TCTTATCAGA TAAGAAAATT GGCACCTACG TAGAGGTGGA TATGTATGGG
TTGCCCACTG ACACCATACG TAAGGAATTC CGAACTCGCA TGGTTATGAA TAATGGACTC
AATCCAGTTT ACAATGAAGA GTCATTTGTA TTTCGGAAGG TGATCCTGCC GGACCTGGCT
GTCTTGAGAA TAGCTGTGTA TGATGATAAC AACAAGCTGA TTGGCCAGAG GATCCTCCCG
CTTGATGGCC TCCAAGCCGG ATATCGACAC ATTTCCCTTC GAAATGAGGG AAATAAACCA
TTATCACTAC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
GGAGATATCG TGGATGCTTT ATCAGATCCA AAGAAATTTC TCTCAATTAC AGAAAAGAGA
GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCCGACGT GCCCAGTGAC
ACTTCCAAAA ATGACAAGAA AGGAAAGGCC AACACCGCCA AAGCAAATGT GACCCCTCAG
AGTAGCTCTG AGCTCAGACC AACCACCACG GCTGCCCTGG CCTCTGGTGT GGAAGCCAAG
AAAGGTATTG AACTTATCCC TCAAGTAAGG ATAGAAGACT TAAAGCAGAT GAAGGCTTAC
TTGAAGCATT TAAAGAAACA GCAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCAAAG
GAACACAGTA CCATGCAGAA GTTACACTGC ACGCAAGTTG ACAAAATTGT GGCACAGTAT
GACAAAGAGA AGTCGACTCA TGAGAAAATC CTAGAGAAGG CAATGAAGAA GAAGGGGGGA
AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATCAAAA TTCAGACGCT GACATCAGAT
CACAAATCTA AGGTCAAAGA GATTGTAGCA CAGCACACAA AGGAATGGTC AGAAATGATC
AATACCCACA GTGCTGAGGA GCAAGAAATC CGAGACCTGC ACCTCAGCCA GCAGTGTGAG
CTGCTGAAAA AGCTACTCAT CAATGCCCAC GAGCAGCAAA CCCAGCAGCT GAAACTGTCC
CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAATAGC
AAAGCCATCA GCCAAGATAA ATCTATCAAG AATAAAGCAG AACGGGAAAG GCGAGTCAGG
GAGTTAAACA GCAGCAACAC TAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCCATGAAG
CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACATCTAGA ATTCCTAGAG
AAACAGAATG AGCAGCTTTT GAAATCCTGT CATGCAGTGT CCCAAACGCA AGGCGAAGGA
GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTATG
AAACTCCAAA ATGCAAACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006723632.1
CDS353..3973
Misc Feature(1)401..781
Misc Feature(2)order(416..418,422..424,458..460,509..511,701..703)
Misc Feature(3)order(425..427,431..433,449..451,455..457,461..463,506..511)
Misc Feature(4)1004..2809
Misc Feature(5)1013..1288
Misc Feature(6)1286..2392
Misc Feature(7)order(1334..1339,1424..1426,1430..1432,1475..1477,1577..1579,1733..1735,1739..1741,2168..2170,2249..2251,2255..2257)
Misc Feature(8)order(1334..1336,1475..1477)
Misc Feature(9)order(1337..1339,1424..1426,1430..1432,1577..1579)
Misc Feature(10)2492..2842
Misc Feature(11)order(2543..2545,2621..2623,2714..2716,2720..2722,2726..2728,2732..2734)
Misc Feature(12)3128..3250
Misc Feature(13)3173..3886
Translation

Target ORF information:

RefSeq Version XM_006723569.2
Organism Homo sapiens(Human)
Definition Homo sapiens phospholipase C beta 4 (PLCB4), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006723569.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGCTTCAGAT
GACCTTGAAC ATGAAAACAA CAAAAAGGGC CTGGTCACTG TAGAAGATGA GCAGGCGTGG
ATGGCATCTT ATAAATATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG
ATCAACTACG CCCAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCAGAAGA ACGCAATATT
CATTATAACA TGTCTTCTTT TAATGAATCA GTCGGTCTTG GCTACTTGAA GACACATGCA
ATTGAATTTG TCAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGA
GTCGATTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCA
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
GGATCGTGCG GGTACCTTCT CAAACCAGAT TTCATGAGGC GGCCTGATCG AACATTTGAC
CCCTTCTCTG AAACTCCTGT TGATGGTGTT ATTGCAGCCA CTTGCTCAGT GCAGGTTATA
TCAGGTCAAT TCTTATCAGA TAAGAAAATT GGCACCTACG TAGAGGTGGA TATGTATGGG
TTGCCCACTG ACACCATACG TAAGGAATTC CGAACTCGCA TGGTTATGAA TAATGGACTC
AATCCAGTTT ACAATGAAGA GTCATTTGTA TTTCGGAAGG TGATCCTGCC GGACCTGGCT
GTCTTGAGAA TAGCTGTGTA TGATGATAAC AACAAGCTGA TTGGCCAGAG GATCCTCCCG
CTTGATGGCC TCCAAGCCGG ATATCGACAC ATTTCCCTTC GAAATGAGGG AAATAAACCA
TTATCACTAC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
GGAGATATCG TGGATGCTTT ATCAGATCCA AAGAAATTTC TCTCAATTAC AGAAAAGAGA
GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCCGACGT GCCCAGTGAC
ACTTCCAAAA ATGACAAGAA AGGAAAGGCC AACACCGCCA AAGCAAATGT GACCCCTCAG
AGTAGCTCTG AGCTCAGACC AACCACCACG GCTGCCCTGG CCTCTGGTGT GGAAGCCAAG
AAAGGTATTG AACTTATCCC TCAAGTAAGG ATAGAAGACT TAAAGCAGAT GAAGGCTTAC
TTGAAGCATT TAAAGAAACA GCAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCAAAG
GAACACAGTA CCATGCAGAA GTTACACTGC ACGCAAGTTG ACAAAATTGT GGCACAGTAT
GACAAAGAGA AGTCGACTCA TGAGAAAATC CTAGAGAAGG CAATGAAGAA GAAGGGGGGA
AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATCAAAA TTCAGACGCT GACATCAGAT
CACAAATCTA AGGTCAAAGA GATTGTAGCA CAGCACACAA AGGAATGGTC AGAAATGATC
AATACCCACA GTGCTGAGGA GCAAGAAATC CGAGACCTGC ACCTCAGCCA GCAGTGTGAG
CTGCTGAAAA AGCTACTCAT CAATGCCCAC GAGCAGCAAA CCCAGCAGCT GAAACTGTCC
CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAATAGC
AAAGCCATCA GCCAAGATAA ATCTATCAAG AATAAAGCAG AACGGGAAAG GCGAGTCAGG
GAGTTAAACA GCAGCAACAC TAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCCATGAAG
CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACATCTAGA ATTCCTAGAG
AAACAGAATG AGCAGCTTTT GAAATCCTGT CATGCAGTGT CCCAAACGCA AGGCGAAGGA
GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTATG
AAACTCCAAA ATGCAAACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu52719
Clone ID Related Accession (Same CDS sequence) XM_006723569.2 , XM_005260724.2 , XM_006723568.3
Accession Version XM_005260724.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3621bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005260724.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGCTTCAGAT
GACCTTGAAC ATGAAAACAA CAAAAAGGGC CTGGTCACTG TAGAAGATGA GCAGGCGTGG
ATGGCATCTT ATAAATATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG
ATCAACTACG CCCAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCAGAAGA ACGCAATATT
CATTATAACA TGTCTTCTTT TAATGAATCA GTCGGTCTTG GCTACTTGAA GACACATGCA
ATTGAATTTG TCAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGA
GTCGATTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCA
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
GGATCGTGCG GGTACCTTCT CAAACCAGAT TTCATGAGGC GGCCTGATCG AACATTTGAC
CCCTTCTCTG AAACTCCTGT TGATGGTGTT ATTGCAGCCA CTTGCTCAGT GCAGGTTATA
TCAGGTCAAT TCTTATCAGA TAAGAAAATT GGCACCTACG TAGAGGTGGA TATGTATGGG
TTGCCCACTG ACACCATACG TAAGGAATTC CGAACTCGCA TGGTTATGAA TAATGGACTC
AATCCAGTTT ACAATGAAGA GTCATTTGTA TTTCGGAAGG TGATCCTGCC GGACCTGGCT
GTCTTGAGAA TAGCTGTGTA TGATGATAAC AACAAGCTGA TTGGCCAGAG GATCCTCCCG
CTTGATGGCC TCCAAGCCGG ATATCGACAC ATTTCCCTTC GAAATGAGGG AAATAAACCA
TTATCACTAC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
GGAGATATCG TGGATGCTTT ATCAGATCCA AAGAAATTTC TCTCAATTAC AGAAAAGAGA
GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCCGACGT GCCCAGTGAC
ACTTCCAAAA ATGACAAGAA AGGAAAGGCC AACACCGCCA AAGCAAATGT GACCCCTCAG
AGTAGCTCTG AGCTCAGACC AACCACCACG GCTGCCCTGG CCTCTGGTGT GGAAGCCAAG
AAAGGTATTG AACTTATCCC TCAAGTAAGG ATAGAAGACT TAAAGCAGAT GAAGGCTTAC
TTGAAGCATT TAAAGAAACA GCAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCAAAG
GAACACAGTA CCATGCAGAA GTTACACTGC ACGCAAGTTG ACAAAATTGT GGCACAGTAT
GACAAAGAGA AGTCGACTCA TGAGAAAATC CTAGAGAAGG CAATGAAGAA GAAGGGGGGA
AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATCAAAA TTCAGACGCT GACATCAGAT
CACAAATCTA AGGTCAAAGA GATTGTAGCA CAGCACACAA AGGAATGGTC AGAAATGATC
AATACCCACA GTGCTGAGGA GCAAGAAATC CGAGACCTGC ACCTCAGCCA GCAGTGTGAG
CTGCTGAAAA AGCTACTCAT CAATGCCCAC GAGCAGCAAA CCCAGCAGCT GAAACTGTCC
CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAATAGC
AAAGCCATCA GCCAAGATAA ATCTATCAAG AATAAAGCAG AACGGGAAAG GCGAGTCAGG
GAGTTAAACA GCAGCAACAC TAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCCATGAAG
CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACATCTAGA ATTCCTAGAG
AAACAGAATG AGCAGCTTTT GAAATCCTGT CATGCAGTGT CCCAAACGCA AGGCGAAGGA
GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTATG
AAACTCCAAA ATGCAAACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005260781.1
CDS273..3893
Misc Feature(1)321..701
Misc Feature(2)order(336..338,342..344,378..380,429..431,621..623)
Misc Feature(3)order(345..347,351..353,369..371,375..377,381..383,426..431)
Misc Feature(4)924..2729
Misc Feature(5)933..1208
Misc Feature(6)1206..2312
Misc Feature(7)order(1254..1259,1344..1346,1350..1352,1395..1397,1497..1499,1653..1655,1659..1661,2088..2090,2169..2171,2175..2177)
Misc Feature(8)order(1254..1256,1395..1397)
Misc Feature(9)order(1257..1259,1344..1346,1350..1352,1497..1499)
Misc Feature(10)2412..2762
Misc Feature(11)order(2463..2465,2541..2543,2634..2636,2640..2642,2646..2648,2652..2654)
Misc Feature(12)3048..3170
Misc Feature(13)3093..3806
Translation

Target ORF information:

RefSeq Version XM_005260724.2
Organism Homo sapiens(Human)
Definition Homo sapiens phospholipase C beta 4 (PLCB4), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005260724.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGCTTCAGAT
GACCTTGAAC ATGAAAACAA CAAAAAGGGC CTGGTCACTG TAGAAGATGA GCAGGCGTGG
ATGGCATCTT ATAAATATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG
ATCAACTACG CCCAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCAGAAGA ACGCAATATT
CATTATAACA TGTCTTCTTT TAATGAATCA GTCGGTCTTG GCTACTTGAA GACACATGCA
ATTGAATTTG TCAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGA
GTCGATTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCA
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
GGATCGTGCG GGTACCTTCT CAAACCAGAT TTCATGAGGC GGCCTGATCG AACATTTGAC
CCCTTCTCTG AAACTCCTGT TGATGGTGTT ATTGCAGCCA CTTGCTCAGT GCAGGTTATA
TCAGGTCAAT TCTTATCAGA TAAGAAAATT GGCACCTACG TAGAGGTGGA TATGTATGGG
TTGCCCACTG ACACCATACG TAAGGAATTC CGAACTCGCA TGGTTATGAA TAATGGACTC
AATCCAGTTT ACAATGAAGA GTCATTTGTA TTTCGGAAGG TGATCCTGCC GGACCTGGCT
GTCTTGAGAA TAGCTGTGTA TGATGATAAC AACAAGCTGA TTGGCCAGAG GATCCTCCCG
CTTGATGGCC TCCAAGCCGG ATATCGACAC ATTTCCCTTC GAAATGAGGG AAATAAACCA
TTATCACTAC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
GGAGATATCG TGGATGCTTT ATCAGATCCA AAGAAATTTC TCTCAATTAC AGAAAAGAGA
GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCCGACGT GCCCAGTGAC
ACTTCCAAAA ATGACAAGAA AGGAAAGGCC AACACCGCCA AAGCAAATGT GACCCCTCAG
AGTAGCTCTG AGCTCAGACC AACCACCACG GCTGCCCTGG CCTCTGGTGT GGAAGCCAAG
AAAGGTATTG AACTTATCCC TCAAGTAAGG ATAGAAGACT TAAAGCAGAT GAAGGCTTAC
TTGAAGCATT TAAAGAAACA GCAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCAAAG
GAACACAGTA CCATGCAGAA GTTACACTGC ACGCAAGTTG ACAAAATTGT GGCACAGTAT
GACAAAGAGA AGTCGACTCA TGAGAAAATC CTAGAGAAGG CAATGAAGAA GAAGGGGGGA
AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATCAAAA TTCAGACGCT GACATCAGAT
CACAAATCTA AGGTCAAAGA GATTGTAGCA CAGCACACAA AGGAATGGTC AGAAATGATC
AATACCCACA GTGCTGAGGA GCAAGAAATC CGAGACCTGC ACCTCAGCCA GCAGTGTGAG
CTGCTGAAAA AGCTACTCAT CAATGCCCAC GAGCAGCAAA CCCAGCAGCT GAAACTGTCC
CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAATAGC
AAAGCCATCA GCCAAGATAA ATCTATCAAG AATAAAGCAG AACGGGAAAG GCGAGTCAGG
GAGTTAAACA GCAGCAACAC TAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCCATGAAG
CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACATCTAGA ATTCCTAGAG
AAACAGAATG AGCAGCTTTT GAAATCCTGT CATGCAGTGT CCCAAACGCA AGGCGAAGGA
GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTATG
AAACTCCAAA ATGCAAACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu52719
Clone ID Related Accession (Same CDS sequence) XM_006723569.2 , XM_005260724.2 , XM_006723568.3
Accession Version XM_006723568.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3621bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006723568.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGCTTCAGAT
GACCTTGAAC ATGAAAACAA CAAAAAGGGC CTGGTCACTG TAGAAGATGA GCAGGCGTGG
ATGGCATCTT ATAAATATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG
ATCAACTACG CCCAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCAGAAGA ACGCAATATT
CATTATAACA TGTCTTCTTT TAATGAATCA GTCGGTCTTG GCTACTTGAA GACACATGCA
ATTGAATTTG TCAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGA
GTCGATTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCA
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
GGATCGTGCG GGTACCTTCT CAAACCAGAT TTCATGAGGC GGCCTGATCG AACATTTGAC
CCCTTCTCTG AAACTCCTGT TGATGGTGTT ATTGCAGCCA CTTGCTCAGT GCAGGTTATA
TCAGGTCAAT TCTTATCAGA TAAGAAAATT GGCACCTACG TAGAGGTGGA TATGTATGGG
TTGCCCACTG ACACCATACG TAAGGAATTC CGAACTCGCA TGGTTATGAA TAATGGACTC
AATCCAGTTT ACAATGAAGA GTCATTTGTA TTTCGGAAGG TGATCCTGCC GGACCTGGCT
GTCTTGAGAA TAGCTGTGTA TGATGATAAC AACAAGCTGA TTGGCCAGAG GATCCTCCCG
CTTGATGGCC TCCAAGCCGG ATATCGACAC ATTTCCCTTC GAAATGAGGG AAATAAACCA
TTATCACTAC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
GGAGATATCG TGGATGCTTT ATCAGATCCA AAGAAATTTC TCTCAATTAC AGAAAAGAGA
GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCCGACGT GCCCAGTGAC
ACTTCCAAAA ATGACAAGAA AGGAAAGGCC AACACCGCCA AAGCAAATGT GACCCCTCAG
AGTAGCTCTG AGCTCAGACC AACCACCACG GCTGCCCTGG CCTCTGGTGT GGAAGCCAAG
AAAGGTATTG AACTTATCCC TCAAGTAAGG ATAGAAGACT TAAAGCAGAT GAAGGCTTAC
TTGAAGCATT TAAAGAAACA GCAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCAAAG
GAACACAGTA CCATGCAGAA GTTACACTGC ACGCAAGTTG ACAAAATTGT GGCACAGTAT
GACAAAGAGA AGTCGACTCA TGAGAAAATC CTAGAGAAGG CAATGAAGAA GAAGGGGGGA
AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATCAAAA TTCAGACGCT GACATCAGAT
CACAAATCTA AGGTCAAAGA GATTGTAGCA CAGCACACAA AGGAATGGTC AGAAATGATC
AATACCCACA GTGCTGAGGA GCAAGAAATC CGAGACCTGC ACCTCAGCCA GCAGTGTGAG
CTGCTGAAAA AGCTACTCAT CAATGCCCAC GAGCAGCAAA CCCAGCAGCT GAAACTGTCC
CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAATAGC
AAAGCCATCA GCCAAGATAA ATCTATCAAG AATAAAGCAG AACGGGAAAG GCGAGTCAGG
GAGTTAAACA GCAGCAACAC TAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCCATGAAG
CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACATCTAGA ATTCCTAGAG
AAACAGAATG AGCAGCTTTT GAAATCCTGT CATGCAGTGT CCCAAACGCA AGGCGAAGGA
GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTATG
AAACTCCAAA ATGCAAACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006723631.1
CDS255..3875
Misc Feature(1)303..683
Misc Feature(2)order(318..320,324..326,360..362,411..413,603..605)
Misc Feature(3)order(327..329,333..335,351..353,357..359,363..365,408..413)
Misc Feature(4)906..2711
Misc Feature(5)915..1190
Misc Feature(6)1188..2294
Misc Feature(7)order(1236..1241,1326..1328,1332..1334,1377..1379,1479..1481,1635..1637,1641..1643,2070..2072,2151..2153,2157..2159)
Misc Feature(8)order(1236..1238,1377..1379)
Misc Feature(9)order(1239..1241,1326..1328,1332..1334,1479..1481)
Misc Feature(10)2394..2744
Misc Feature(11)order(2445..2447,2523..2525,2616..2618,2622..2624,2628..2630,2634..2636)
Misc Feature(12)3030..3152
Misc Feature(13)3075..3788
Translation

Target ORF information:

RefSeq Version XM_006723568.3
Organism Homo sapiens(Human)
Definition Homo sapiens phospholipase C beta 4 (PLCB4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006723568.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGGCCAAAC CTTATGAATT TAACTGGCAG AAGGAAGTTC CCTCCTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATACGA GGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
GTGGATGAGT TTGGCTTCTT TCTGACATGG AGAAGTGAAG GCAAGGAAGG ACAGGTGCTA
GAATGCTCCC TCATCAACAG TATTCGGTCG GGAGCCATAC CAAAGGATCC CAAAATCTTG
GCTGCTCTTG AAGCTGTTGG AAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGTGTC
TGCAGTGGCA CAGATCTAGT GAACATTAGT TTTACCTACA TGGTGGCTGA AAATCCAGAA
GTAACTAAGC AATGGGTAGA AGGCCTGAGA TCAATCATAC ACAACTTCAG GGCCAACAAC
GTCAGTCCAA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
AATGGTAAAA TTCCAGTTAG GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGCTTCAGAT
GACCTTGAAC ATGAAAACAA CAAAAAGGGC CTGGTCACTG TAGAAGATGA GCAGGCGTGG
ATGGCATCTT ATAAATATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG
ATCAACTACG CCCAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCAGAAGA ACGCAATATT
CATTATAACA TGTCTTCTTT TAATGAATCA GTCGGTCTTG GCTACTTGAA GACACATGCA
ATTGAATTTG TCAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGA
GTCGATTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCA
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
GGATCGTGCG GGTACCTTCT CAAACCAGAT TTCATGAGGC GGCCTGATCG AACATTTGAC
CCCTTCTCTG AAACTCCTGT TGATGGTGTT ATTGCAGCCA CTTGCTCAGT GCAGGTTATA
TCAGGTCAAT TCTTATCAGA TAAGAAAATT GGCACCTACG TAGAGGTGGA TATGTATGGG
TTGCCCACTG ACACCATACG TAAGGAATTC CGAACTCGCA TGGTTATGAA TAATGGACTC
AATCCAGTTT ACAATGAAGA GTCATTTGTA TTTCGGAAGG TGATCCTGCC GGACCTGGCT
GTCTTGAGAA TAGCTGTGTA TGATGATAAC AACAAGCTGA TTGGCCAGAG GATCCTCCCG
CTTGATGGCC TCCAAGCCGG ATATCGACAC ATTTCCCTTC GAAATGAGGG AAATAAACCA
TTATCACTAC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
GGAGATATCG TGGATGCTTT ATCAGATCCA AAGAAATTTC TCTCAATTAC AGAAAAGAGA
GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCCGACGT GCCCAGTGAC
ACTTCCAAAA ATGACAAGAA AGGAAAGGCC AACACCGCCA AAGCAAATGT GACCCCTCAG
AGTAGCTCTG AGCTCAGACC AACCACCACG GCTGCCCTGG CCTCTGGTGT GGAAGCCAAG
AAAGGTATTG AACTTATCCC TCAAGTAAGG ATAGAAGACT TAAAGCAGAT GAAGGCTTAC
TTGAAGCATT TAAAGAAACA GCAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCAAAG
GAACACAGTA CCATGCAGAA GTTACACTGC ACGCAAGTTG ACAAAATTGT GGCACAGTAT
GACAAAGAGA AGTCGACTCA TGAGAAAATC CTAGAGAAGG CAATGAAGAA GAAGGGGGGA
AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATCAAAA TTCAGACGCT GACATCAGAT
CACAAATCTA AGGTCAAAGA GATTGTAGCA CAGCACACAA AGGAATGGTC AGAAATGATC
AATACCCACA GTGCTGAGGA GCAAGAAATC CGAGACCTGC ACCTCAGCCA GCAGTGTGAG
CTGCTGAAAA AGCTACTCAT CAATGCCCAC GAGCAGCAAA CCCAGCAGCT GAAACTGTCC
CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAATAGC
AAAGCCATCA GCCAAGATAA ATCTATCAAG AATAAAGCAG AACGGGAAAG GCGAGTCAGG
GAGTTAAACA GCAGCAACAC TAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCCATGAAG
CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACATCTAGA ATTCCTAGAG
AAACAGAATG AGCAGCTTTT GAAATCCTGT CATGCAGTGT CCCAAACGCA AGGCGAAGGA
GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTATG
AAACTCCAAA ATGCAAACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59763
Clone ID Related Accession (Same CDS sequence) XM_011529254.2
Accession Version XM_011529254.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3141bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X7
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011529254.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGAAAAACA TCTGCAATGA GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG 
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGCTTCAGAT
GACCTTGAAC ATGAAAACAA CAAAAAGGGC CTGGTCACTG TAGAAGATGA GCAGGCGTGG
ATGGCATCTT ATAAATATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG
ATCAACTACG CCCAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCAGAAGA ACGCAATATT
CATTATAACA TGTCTTCTTT TAATGAATCA GTCGGTCTTG GCTACTTGAA GACACATGCA
ATTGAATTTG TCAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGA
GTCGATTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCA
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
GGATCGTGCG GGTACCTTCT CAAACCAGAT TTCATGAGGC GGCCTGATCG AACATTTGAC
CCCTTCTCTG AAACTCCTGT TGATGGTGTT ATTGCAGCCA CTTGCTCAGT GCAGGTTATA
TCAGGTCAAT TCTTATCAGA TAAGAAAATT GGCACCTACG TAGAGGTGGA TATGTATGGG
TTGCCCACTG ACACCATACG TAAGGAATTC CGAACTCGCA TGGTTATGAA TAATGGACTC
AATCCAGTTT ACAATGAAGA GTCATTTGTA TTTCGGAAGG TGATCCTGCC GGACCTGGCT
GTCTTGAGAA TAGCTGTGTA TGATGATAAC AACAAGCTGA TTGGCCAGAG GATCCTCCCG
CTTGATGGCC TCCAAGCCGG ATATCGACAC ATTTCCCTTC GAAATGAGGG AAATAAACCA
TTATCACTAC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
GGAGATATCG TGGATGCTTT ATCAGATCCA AAGAAATTTC TCTCAATTAC AGAAAAGAGA
GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCCGACGT GCCCAGTGAC
ACTTCCAAAA ATGACAAGAA AGGAAAGGCC AACACCGCCA AAGCAAATGT GACCCCTCAG
AGTAGCTCTG AGCTCAGACC AACCACCACG GCTGCCCTGG CCTCTGGTGT GGAAGCCAAG
AAAGGTATTG AACTTATCCC TCAAGTAAGG ATAGAAGACT TAAAGCAGAT GAAGGCTTAC
TTGAAGCATT TAAAGAAACA GCAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCAAAG
GAACACAGTA CCATGCAGAA GTTACACTGC ACGCAAGTTG ACAAAATTGT GGCACAGTAT
GACAAAGAGA AGTCGACTCA TGAGAAAATC CTAGAGAAGG CAATGAAGAA GAAGGGGGGA
AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATCAAAA TTCAGACGCT GACATCAGAT
CACAAATCTA AGGTCAAAGA GATTGTAGCA CAGCACACAA AGGAATGGTC AGAAATGATC
AATACCCACA GTGCTGAGGA GCAAGAAATC CGAGACCTGC ACCTCAGCCA GCAGTGTGAG
CTGCTGAAAA AGCTACTCAT CAATGCCCAC GAGCAGCAAA CCCAGCAGCT GAAACTGTCC
CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAATAGC
AAAGCCATCA GCCAAGATAA ATCTATCAAG AATAAAGCAG AACGGGAAAG GCGAGTCAGG
GAGTTAAACA GCAGCAACAC TAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCCATGAAG
CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACATCTAGA ATTCCTAGAG
AAACAGAATG AGCAGCTTTT GAAATCCTGT CATGCAGTGT CCCAAACGCA AGGCGAAGGA
GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTATG
AAACTCCAAA ATGCAAACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011527556.1
CDS3855..6995
Misc Feature(1)4026..5831
Misc Feature(2)4035..4310
Misc Feature(3)4308..5414
Misc Feature(4)order(4356..4361,4446..4448,4452..4454,4497..4499,4599..4601,4755..4757,4761..4763,5190..5192,5271..5273,5277..5279)
Misc Feature(5)order(4356..4358,4497..4499)
Misc Feature(6)order(4359..4361,4446..4448,4452..4454,4599..4601)
Misc Feature(7)5514..5864
Misc Feature(8)order(5565..5567,5643..5645,5736..5738,5742..5744,5748..5750,5754..5756)
Misc Feature(9)6150..6272
Misc Feature(10)6195..6908
Translation

Target ORF information:

RefSeq Version XM_011529254.2
Organism Homo sapiens(Human)
Definition Homo sapiens phospholipase C beta 4 (PLCB4), transcript variant X10, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011529254.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGAAAAACA TCTGCAATGA GAGTATTACT AGAACATTTG CATCGGGAAA AACAGAAAAG 
GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
CCCACAGCAT TTTCTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
GATATAGAAG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTT
CCATTTTATG ATGCCAAAAG GGCAATGCAG ATCATTGAGA TGTATGAACC TGATGAAGAT
TTGAAGAAAA AAGGCCTTAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
AACGCCCCAG TCTTCCTAGA TCGTTTAGAA CTTTACCAAG AAATGGACCA TCCTCTGGCT
CACTACTTCA TCAGTTCTTC CCATAACACT TATCTCACTG GCAGACAGTT CGGCGGGAAG
TCTTCGGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
TGCTGGGATG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGT
ACAGATATCC TTTTTAAGGA TGTAATTCAA GCCATCAAGG AAACTGCATT TGTCACATCA
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
TCCAAATATT GCGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
CCACTTGAAC CAGGCAGGGC TTTGCCATCC CCCAATGACC TCAAAAGAAA AATACTCATA
AAAAACAAGC GGCTGAAACC TGAAGTTGAA AAAAAACAGC TGGAAGCTTT GAGAAGCATG
ATGGAAGCTG GAGAATCTGC CTCCCCAGCA AACATCTTAG AGGACGATAA TGAAGAGGAG
ATCGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCCGAAT TCAAATTTGG AAATGAACTT
TCTGCTGATG ACTTGGGTCA CAAGGAAGCT GTTGCAAATA GCGTCAAGAA GGCTTCAGAT
GACCTTGAAC ATGAAAACAA CAAAAAGGGC CTGGTCACTG TAGAAGATGA GCAGGCGTGG
ATGGCATCTT ATAAATATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCCACAATG
ATCAACTACG CCCAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCAGAAGA ACGCAATATT
CATTATAACA TGTCTTCTTT TAATGAATCA GTCGGTCTTG GCTACTTGAA GACACATGCA
ATTGAATTTG TCAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGA
GTCGATTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCA
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
GGATCGTGCG GGTACCTTCT CAAACCAGAT TTCATGAGGC GGCCTGATCG AACATTTGAC
CCCTTCTCTG AAACTCCTGT TGATGGTGTT ATTGCAGCCA CTTGCTCAGT GCAGGTTATA
TCAGGTCAAT TCTTATCAGA TAAGAAAATT GGCACCTACG TAGAGGTGGA TATGTATGGG
TTGCCCACTG ACACCATACG TAAGGAATTC CGAACTCGCA TGGTTATGAA TAATGGACTC
AATCCAGTTT ACAATGAAGA GTCATTTGTA TTTCGGAAGG TGATCCTGCC GGACCTGGCT
GTCTTGAGAA TAGCTGTGTA TGATGATAAC AACAAGCTGA TTGGCCAGAG GATCCTCCCG
CTTGATGGCC TCCAAGCCGG ATATCGACAC ATTTCCCTTC GAAATGAGGG AAATAAACCA
TTATCACTAC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
GGAGATATCG TGGATGCTTT ATCAGATCCA AAGAAATTTC TCTCAATTAC AGAAAAGAGA
GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCCGACGT GCCCAGTGAC
ACTTCCAAAA ATGACAAGAA AGGAAAGGCC AACACCGCCA AAGCAAATGT GACCCCTCAG
AGTAGCTCTG AGCTCAGACC AACCACCACG GCTGCCCTGG CCTCTGGTGT GGAAGCCAAG
AAAGGTATTG AACTTATCCC TCAAGTAAGG ATAGAAGACT TAAAGCAGAT GAAGGCTTAC
TTGAAGCATT TAAAGAAACA GCAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCAAAG
GAACACAGTA CCATGCAGAA GTTACACTGC ACGCAAGTTG ACAAAATTGT GGCACAGTAT
GACAAAGAGA AGTCGACTCA TGAGAAAATC CTAGAGAAGG CAATGAAGAA GAAGGGGGGA
AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATCAAAA TTCAGACGCT GACATCAGAT
CACAAATCTA AGGTCAAAGA GATTGTAGCA CAGCACACAA AGGAATGGTC AGAAATGATC
AATACCCACA GTGCTGAGGA GCAAGAAATC CGAGACCTGC ACCTCAGCCA GCAGTGTGAG
CTGCTGAAAA AGCTACTCAT CAATGCCCAC GAGCAGCAAA CCCAGCAGCT GAAACTGTCC
CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAATAGC
AAAGCCATCA GCCAAGATAA ATCTATCAAG AATAAAGCAG AACGGGAAAG GCGAGTCAGG
GAGTTAAACA GCAGCAACAC TAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCCATGAAG
CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACATCTAGA ATTCCTAGAG
AAACAGAATG AGCAGCTTTT GAAATCCTGT CATGCAGTGT CCCAAACGCA AGGCGAAGGA
GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTATG
AAACTCCAAA ATGCAAACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Deep sequencing of uveal melanoma identifies a recurrent mutation in PLCB4.
Oncotarget7(4)4624-31(2016 Jan)
Johansson P,Aoude LG,Wadt K,Glasson WJ,Warrier SK,Hewitt AW,Kiilgaard JF,Heegaard S,Isaacs T,Franchina M,Ingvar C,Vermeulen T,Whitehead KJ,Schmidt CW,Palmer JM,Symmons J,Gerdes AM,Jönsson G,Hayward NK


book

Genetic variants in PLCB4/PLCB1 as susceptibility loci for coronary artery aneurysm formation in Kawasaki disease in Han Chinese in Taiwan.
Scientific reports514762(2015 Oct)
Lin YJ,Chang JS,Liu X,Tsang H,Chien WK,Chen JH,Hsieh HY,Hsueh KC,Shiao YT,Li JP,Lin CW,Lai CH,Wu JY,Chen CH,Lin JG,Lin TH,Liao CC,Huang SM,Lan YC,Ho TJ,Liang WM,Yeh YC,Lin JC,Tsai FJ


book

Gene expression profile in fibroblasts of Huntington's disease patients and controls.
Journal of the neurological sciences337(1-2)42-6(2014 Feb)
Marchina E,Misasi S,Bozzato A,Ferraboli S,Agosti C,Rozzini L,Borsani G,Barlati S,Padovani A


book

Phospholipase C-β1 and β4 contribute to non-genetic cell-to-cell variability in histamine-induced calcium signals in HeLa cells.
PloS one9(1)e86410(2014)
Ishida S,Matsu-Ura T,Fukami K,Michikawa T,Mikoshiba K


book

Further characterization of atypical features in auriculocondylar syndrome caused by recessive PLCB4 mutations.
American journal of medical genetics. Part A161A(9)2339-46(2013 Sep)
Kido Y,Gordon CT,Sakazume S,Ben Bdira E,Dattani M,Wilson LC,Lyonnet S,Murakami N,Cunningham ML,Amiel J,Nagai T


 
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