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POLH cDNA ORF clone, Homo sapiens (human)

Gene Symbol POLH
Entrez Gene ID 5429
Full Name polymerase (DNA directed), eta
Synonyms RAD30, RAD30A, XP-V, XPV
General protein information
Preferred Names
DNA polymerase eta
Names
DNA polymerase eta
RAD30 homolog A
xeroderma pigmentosum variant type protein
NP_006493.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

6

6p21.1

Summary This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]. lac of sum
Disorder MIM:

603968

Disorder Html: Xeroderma pigmentosum, variant type, 278750 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_005249186 XP_005249243 DNA polymerase eta isoform X2
XM_011514698 XP_011513000 DNA polymerase eta isoform X1
NM_006502 NP_006493 DNA polymerase eta isoform 1
NM_001291969 NP_001278898 DNA polymerase eta isoform 2
NM_001291970 NP_001278899 DNA polymerase eta isoform 3



Homo sapiens (human) POLH NP_006493.1
Pan troglodytes (chimpanzee) POLH XP_518497.1
Macaca mulatta (Rhesus monkey) POLH XP_002803805.1
Canis lupus familiaris (dog) POLH XP_532150.2
Bos taurus (cattle) POLH NP_001029622.1
Mus musculus (house mouse) Polh NP_109640.1
Rattus norvegicus (Norway rat) Polh NP_001101674.1
Gallus gallus (chicken) POLH NP_001001304.1
Danio rerio (zebrafish) polh NP_001035337.1
Caenorhabditis elegans polh-1 NP_497480.2
Xenopus (Silurana) tropicalis (western clawed frog) polh XP_002942198.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following POLH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu40354 XM_005249186 PREDICTED: Homo sapiens polymerase (DNA directed), eta (POLH), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $307.30
$439.00
OHu24571 XM_011514698 PREDICTED: Homo sapiens polymerase (DNA directed), eta (POLH), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu24497
NM_006502 Homo sapiens polymerase (DNA directed), eta (POLH), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$279.50-$391.30
$559.00
OHu24571 NM_001291969 Homo sapiens polymerase (DNA directed), eta (POLH), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu24572
NM_001291970 Homo sapiens polymerase (DNA directed), eta (POLH), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu40354
Clone ID Related Accession (Same CDS sequence) XM_005249186
Accession Version XM_005249186.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1956bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product DNA polymerase eta isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007592.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530382161. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGTGGGCAG ATGATGCTAA GAAGTTATGT CCAGATCTTC TACTGGCACA AGTTCGTGAG 
TCCCGTGGGA AAGCTAACCT CACCAAGTAC CGGGAAGCCA GTGTTGAAGT GATGGAGATA
ATGTCTCGTT TTGCTGTGAT TGAACGTGCC AGCATTGATG AGGCTTACGT AGATCTGACC
AGTGCTGTAC AAGAGAGACT ACAAAAGCTA CAAGGTCAGC CTATCTCGGC AGACTTGTTG
CCAAGCACTT ACATTGAAGG GTTGCCCCAA GGCCCTACAA CGGCAGAAGA GACTGTTCAG
AAAGAGGGGA TGCGAAAACA AGGCTTATTT CAATGGCTCG ATTCTCTTCA GATTGATAAC
CTCACCTCTC CAGACCTGCA GCTCACCGTG GGAGCAGTGA TTGTGGAGGA AATGAGAGCA
GCCATAGAGA GGGAGACTGG TTTTCAGTGT TCAGCTGGAA TTTCACACAA TAAGGTCCTG
GCAAAACTGG CCTGTGGACT AAACAAGCCC AACCGCCAAA CCCTGGTTTC ACATGGGTCA
GTCCCACAGC TCTTCAGCCA AATGCCCATT CGCAAAATCC GTAGTCTTGG AGGAAAGCTA
GGGGCCTCTG TCATTGAGAT CCTAGGGATA GAATACATGG GTGAACTGAC CCAGTTCACT
GAATCCCAGC TCCAGAGTCA TTTTGGGGAG AAGAATGGGT CTTGGCTATA TGCCATGTGC
CGAGGGATTG AACATGATCC AGTTAAACCC AGGCAACTAC CCAAAACCAT TGGCTGTAGT
AAGAACTTCC CAGGAAAAAC AGCTCTTGCT ACTCGGGAAC AGGTACAATG GTGGCTGTTG
CAATTAGCCC AGGAACTAGA GGAGAGACTG ACTAAAGACC GAAATGATAA TGACAGGGTA
GCCACCCAGC TGGTTGTGAG CATTCGTGTA CAAGGAGACA AACGCCTCAG CAGCCTGCGC
CGCTGCTGTG CCCTTACCCG CTATGATGCT CACAAGATGA GCCATGATGC ATTTACTGTC
ATCAAGAACT GTAATACTTC TGGAATCCAG ACAGAATGGT CTCCTCCTCT CACAATGCTT
TTCCTCTGTG CTACAAAATT TTCTGCCTCT GCCCCTTCAT CTTCTACAGA CATCACCAGC
TTCTTGAGCA GTGACCCAAG TTCTCTGCCA AAGGTGCCAG TTACCAGCTC AGAAGCTAAG
ACCCAGGGAA GTGGCCCAGC GGTGACAGCC ACTAAGAAAG CAACCACGTC TCTGGAATCA
TTCTTCCAAA AAGCTGCAGA AAGGCAGAAA GTTAAAGAAG CTTCGCTTTC ATCTCTTACT
GCTCCCACTC AGGCTCCCAT GAGCAATTCA CCATCCAAGC CCTCATTACC TTTTCAAACC
AGTCAAAGTA CAGGAACTGA GCCCTTCTTT AAGCAGAAAA GTCTGCTTCT AAAGCAGAAA
CAGCTTAATA ATTCTTCAGT TTCTTCCCCC CAACAAAACC CATGGTCCAA CTGTAAAGCA
TTACCAAACT CTTTACCAAC AGAGTATCCA GGGTGTGTCC CTGTTTGTGA AGGGGTGTCG
AAGCTAGAAG AATCCTCTAA AGCAACTCCT GCAGAGATGG ATTTGGCCCA CAACAGCCAA
AGCATGCACG CCTCTTCAGC TTCCAAATCT GTGCTGGAGG TGACTCAGAA AGCAACCCCA
AATCCAAGTC TTCTAGCTGC TGAGGACCAA GTGCCCTGTG AGAAGTGTGG CTCCCTGGTA
CCGGTATGGG ATATGCCAGA ACACATGGAC TATCATTTTG CATTGGAGTT GCAGAAATCC
TTTTTGCAGC CCCACTCTTC AAACCCCCAG GTTGTTTCTG CCGTATCTCA TCAAGGCAAA
AGAAATCCCA AGAGCCCTTT GGCCTGCACT AATAAACGCC CCAGGCCTGA GGGCATGCAA
ACATTGGAAT CATTTTTTAA GCCATTAACA CATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005249243.1
CDS344..2299
Misc Feature(1)344..1441(+)
Misc Feature(2)494..1315(+)
Translation

Target ORF information:

RefSeq Version XM_005249186
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens polymerase (DNA directed), eta (POLH), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005249186

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGTGGGCAG ATGATGCTAA GAAGTTATGT CCAGATCTTC TACTGGCACA AGTTCGTGAG 
TCCCGTGGGA AAGCTAACCT CACCAAGTAC CGGGAAGCCA GTGTTGAAGT GATGGAGATA
ATGTCTCGTT TTGCTGTGAT TGAACGTGCC AGCATTGATG AGGCTTACGT AGATCTGACC
AGTGCTGTAC AAGAGAGACT ACAAAAGCTA CAAGGTCAGC CTATCTCGGC AGACTTGTTG
CCAAGCACTT ACATTGAAGG GTTGCCCCAA GGCCCTACAA CGGCAGAAGA GACTGTTCAG
AAAGAGGGGA TGCGAAAACA AGGCTTATTT CAATGGCTCG ATTCTCTTCA GATTGATAAC
CTCACCTCTC CAGACCTGCA GCTCACCGTG GGAGCAGTGA TTGTGGAGGA AATGAGAGCA
GCCATAGAGA GGGAGACTGG TTTTCAGTGT TCAGCTGGAA TTTCACACAA TAAGGTCCTG
GCAAAACTGG CCTGTGGACT AAACAAGCCC AACCGCCAAA CCCTGGTTTC ACATGGGTCA
GTCCCACAGC TCTTCAGCCA AATGCCCATT CGCAAAATCC GTAGTCTTGG AGGAAAGCTA
GGGGCCTCTG TCATTGAGAT CCTAGGGATA GAATACATGG GTGAACTGAC CCAGTTCACT
GAATCCCAGC TCCAGAGTCA TTTTGGGGAG AAGAATGGGT CTTGGCTATA TGCCATGTGC
CGAGGGATTG AACATGATCC AGTTAAACCC AGGCAACTAC CCAAAACCAT TGGCTGTAGT
AAGAACTTCC CAGGAAAAAC AGCTCTTGCT ACTCGGGAAC AGGTACAATG GTGGCTGTTG
CAATTAGCCC AGGAACTAGA GGAGAGACTG ACTAAAGACC GAAATGATAA TGACAGGGTA
GCCACCCAGC TGGTTGTGAG CATTCGTGTA CAAGGAGACA AACGCCTCAG CAGCCTGCGC
CGCTGCTGTG CCCTTACCCG CTATGATGCT CACAAGATGA GCCATGATGC ATTTACTGTC
ATCAAGAACT GTAATACTTC TGGAATCCAG ACAGAATGGT CTCCTCCTCT CACAATGCTT
TTCCTCTGTG CTACAAAATT TTCTGCCTCT GCCCCTTCAT CTTCTACAGA CATCACCAGC
TTCTTGAGCA GTGACCCAAG TTCTCTGCCA AAGGTGCCAG TTACCAGCTC AGAAGCTAAG
ACCCAGGGAA GTGGCCCAGC GGTGACAGCC ACTAAGAAAG CAACCACGTC TCTGGAATCA
TTCTTCCAAA AAGCTGCAGA AAGGCAGAAA GTTAAAGAAG CTTCGCTTTC ATCTCTTACT
GCTCCCACTC AGGCTCCCAT GAGCAATTCA CCATCCAAGC CCTCATTACC TTTTCAAACC
AGTCAAAGTA CAGGAACTGA GCCCTTCTTT AAGCAGAAAA GTCTGCTTCT AAAGCAGAAA
CAGCTTAATA ATTCTTCAGT TTCTTCCCCC CAACAAAACC CATGGTCCAA CTGTAAAGCA
TTACCAAACT CTTTACCAAC AGAGTATCCA GGGTGTGTCC CTGTTTGTGA AGGGGTGTCG
AAGCTAGAAG AATCCTCTAA AGCAACTCCT GCAGAGATGG ATTTGGCCCA CAACAGCCAA
AGCATGCACG CCTCTTCAGC TTCCAAATCT GTGCTGGAGG TGACTCAGAA AGCAACCCCA
AATCCAAGTC TTCTAGCTGC TGAGGACCAA GTGCCCTGTG AGAAGTGTGG CTCCCTGGTA
CCGGTATGGG ATATGCCAGA ACACATGGAC TATCATTTTG CATTGGAGTT GCAGAAATCC
TTTTTGCAGC CCCACTCTTC AAACCCCCAG GTTGTTTCTG CCGTATCTCA TCAAGGCAAA
AGAAATCCCA AGAGCCCTTT GGCCTGCACT AATAAACGCC CCAGGCCTGA GGGCATGCAA
ACATTGGAAT CATTTTTTAA GCCATTAACA CATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24571
Clone ID Related Accession (Same CDS sequence) NM_001291969 , XM_011514698
Accession Version XM_011514698.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1770bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product DNA polymerase eta isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007592.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGAAGCTCG TGCATTTGGA GTCACTAGAA GTATGTGGGC AGATGATGCT AAGAAGTTAT 
GTCCAGATCT TCTACTGGCA CAAGTTCGTG AGTCCCGTGG GAAAGCTAAC CTCACCAAAG
GGGATGCGAA AACAAGGCTT ATTTCAATGG CTCGATTCTC TTCAGATTGA TAACCTCACC
TCTCCAGACC TGCAGCTCAC CGTGGGAGCA GTGATTGTGG AGGAAATGAG AGCAGCCATA
GAGAGGGAGA CTGGTTTTCA GTGTTCAGCT GGAATTTCAC ACAATAAGGT CCTGGCAAAA
CTGGCCTGTG GACTAAACAA GCCCAACCGC CAAACCCTGG TTTCACATGG GTCAGTCCCA
CAGCTCTTCA GCCAAATGCC CATTCGCAAA ATCCGTAGTC TTGGAGGAAA GCTAGGGGCC
TCTGTCATTG AGATCCTAGG GATAGAATAC ATGGGTGAAC TGACCCAGTT CACTGAATCC
CAGCTCCAGA GTCATTTTGG GGAGAAGAAT GGGTCTTGGC TATATGCCAT GTGCCGAGGG
ATTGAACATG ATCCAGTTAA ACCCAGGCAA CTACCCAAAA CCATTGGCTG TAGTAAGAAC
TTCCCAGGAA AAACAGCTCT TGCTACTCGG GAACAGGTAC AATGGTGGCT GTTGCAATTA
GCCCAGGAAC TAGAGGAGAG ACTGACTAAA GACCGAAATG ATAATGACAG GGTAGCCACC
CAGCTGGTTG TGAGCATTCG TGTACAAGGA GACAAACGCC TCAGCAGCCT GCGCCGCTGC
TGTGCCCTTA CCCGCTATGA TGCTCACAAG ATGAGCCATG ATGCATTTAC TGTCATCAAG
AACTGTAATA CTTCTGGAAT CCAGACAGAA TGGTCTCCTC CTCTCACAAT GCTTTTCCTC
TGTGCTACAA AATTTTCTGC CTCTGCCCCT TCATCTTCTA CAGACATCAC CAGCTTCTTG
AGCAGTGACC CAAGTTCTCT GCCAAAGGTG CCAGTTACCA GCTCAGAAGC TAAGACCCAG
GGAAGTGGCC CAGCGGTGAC AGCCACTAAG AAAGCAACCA CGTCTCTGGA ATCATTCTTC
CAAAAAGCTG CAGAAAGGCA GAAAGTTAAA GAAGCTTCGC TTTCATCTCT TACTGCTCCC
ACTCAGGCTC CCATGAGCAA TTCACCATCC AAGCCCTCAT TACCTTTTCA AACCAGTCAA
AGTACAGGAA CTGAGCCCTT CTTTAAGCAG AAAAGTCTGC TTCTAAAGCA GAAACAGCTT
AATAATTCTT CAGTTTCTTC CCCCCAACAA AACCCATGGT CCAACTGTAA AGCATTACCA
AACTCTTTAC CAACAGAGTA TCCAGGGTGT GTCCCTGTTT GTGAAGGGGT GTCGAAGCTA
GAAGAATCCT CTAAAGCAAC TCCTGCAGAG ATGGATTTGG CCCACAACAG CCAAAGCATG
CACGCCTCTT CAGCTTCCAA ATCTGTGCTG GAGGTGACTC AGAAAGCAAC CCCAAATCCA
AGTCTTCTAG CTGCTGAGGA CCAAGTGCCC TGTGAGAAGT GTGGCTCCCT GGTACCGGTA
TGGGATATGC CAGAACACAT GGACTATCAT TTTGCATTGG AGTTGCAGAA ATCCTTTTTG
CAGCCCCACT CTTCAAACCC CCAGGTTGTT TCTGCCGTAT CTCATCAAGG CAAAAGAAAT
CCCAAGAGCC CTTTGGCCTG CACTAATAAA CGCCCCAGGC CTGAGGGCAT GCAAACATTG
GAATCATTTT TTAAGCCATT AACACATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011513000.1
CDS444..2213
Misc Feature(1)639..1355(+)
Misc Feature(2)837..1229(+)
Translation

Target ORF information:

RefSeq Version XM_011514698
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens polymerase (DNA directed), eta (POLH), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011514698

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGAAGCTCG TGCATTTGGA GTCACTAGAA GTATGTGGGC AGATGATGCT AAGAAGTTAT 
GTCCAGATCT TCTACTGGCA CAAGTTCGTG AGTCCCGTGG GAAAGCTAAC CTCACCAAAG
GGGATGCGAA AACAAGGCTT ATTTCAATGG CTCGATTCTC TTCAGATTGA TAACCTCACC
TCTCCAGACC TGCAGCTCAC CGTGGGAGCA GTGATTGTGG AGGAAATGAG AGCAGCCATA
GAGAGGGAGA CTGGTTTTCA GTGTTCAGCT GGAATTTCAC ACAATAAGGT CCTGGCAAAA
CTGGCCTGTG GACTAAACAA GCCCAACCGC CAAACCCTGG TTTCACATGG GTCAGTCCCA
CAGCTCTTCA GCCAAATGCC CATTCGCAAA ATCCGTAGTC TTGGAGGAAA GCTAGGGGCC
TCTGTCATTG AGATCCTAGG GATAGAATAC ATGGGTGAAC TGACCCAGTT CACTGAATCC
CAGCTCCAGA GTCATTTTGG GGAGAAGAAT GGGTCTTGGC TATATGCCAT GTGCCGAGGG
ATTGAACATG ATCCAGTTAA ACCCAGGCAA CTACCCAAAA CCATTGGCTG TAGTAAGAAC
TTCCCAGGAA AAACAGCTCT TGCTACTCGG GAACAGGTAC AATGGTGGCT GTTGCAATTA
GCCCAGGAAC TAGAGGAGAG ACTGACTAAA GACCGAAATG ATAATGACAG GGTAGCCACC
CAGCTGGTTG TGAGCATTCG TGTACAAGGA GACAAACGCC TCAGCAGCCT GCGCCGCTGC
TGTGCCCTTA CCCGCTATGA TGCTCACAAG ATGAGCCATG ATGCATTTAC TGTCATCAAG
AACTGTAATA CTTCTGGAAT CCAGACAGAA TGGTCTCCTC CTCTCACAAT GCTTTTCCTC
TGTGCTACAA AATTTTCTGC CTCTGCCCCT TCATCTTCTA CAGACATCAC CAGCTTCTTG
AGCAGTGACC CAAGTTCTCT GCCAAAGGTG CCAGTTACCA GCTCAGAAGC TAAGACCCAG
GGAAGTGGCC CAGCGGTGAC AGCCACTAAG AAAGCAACCA CGTCTCTGGA ATCATTCTTC
CAAAAAGCTG CAGAAAGGCA GAAAGTTAAA GAAGCTTCGC TTTCATCTCT TACTGCTCCC
ACTCAGGCTC CCATGAGCAA TTCACCATCC AAGCCCTCAT TACCTTTTCA AACCAGTCAA
AGTACAGGAA CTGAGCCCTT CTTTAAGCAG AAAAGTCTGC TTCTAAAGCA GAAACAGCTT
AATAATTCTT CAGTTTCTTC CCCCCAACAA AACCCATGGT CCAACTGTAA AGCATTACCA
AACTCTTTAC CAACAGAGTA TCCAGGGTGT GTCCCTGTTT GTGAAGGGGT GTCGAAGCTA
GAAGAATCCT CTAAAGCAAC TCCTGCAGAG ATGGATTTGG CCCACAACAG CCAAAGCATG
CACGCCTCTT CAGCTTCCAA ATCTGTGCTG GAGGTGACTC AGAAAGCAAC CCCAAATCCA
AGTCTTCTAG CTGCTGAGGA CCAAGTGCCC TGTGAGAAGT GTGGCTCCCT GGTACCGGTA
TGGGATATGC CAGAACACAT GGACTATCAT TTTGCATTGG AGTTGCAGAA ATCCTTTTTG
CAGCCCCACT CTTCAAACCC CCAGGTTGTT TCTGCCGTAT CTCATCAAGG CAAAAGAAAT
CCCAAGAGCC CTTTGGCCTG CACTAATAAA CGCCCCAGGC CTGAGGGCAT GCAAACATTG
GAATCATTTT TTAAGCCATT AACACATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24497
Clone ID Related Accession (Same CDS sequence) NM_006502
Accession Version NM_006502.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2142bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product DNA polymerase eta isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC416114.1, AB024313.1 and AL353602.10. This sequence is a reference standard in the RefSeqGene project. On Mar 21, 2008 this sequence version replaced gi:5729981. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB024313.1, AK294430.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGGCTACTG GACAGGATCG AGTGGTTGCT CTCGTGGACA TGGACTGTTT TTTTGTTCAA 
GTGGAGCAGC GGCAAAATCC TCATTTGAGG AATAAACCTT GTGCAGTTGT ACAGTACAAA
TCATGGAAGG GTGGTGGAAT AATTGCAGTG AGTTATGAAG CTCGTGCATT TGGAGTCACT
AGAAGTATGT GGGCAGATGA TGCTAAGAAG TTATGTCCAG ATCTTCTACT GGCACAAGTT
CGTGAGTCCC GTGGGAAAGC TAACCTCACC AAGTACCGGG AAGCCAGTGT TGAAGTGATG
GAGATAATGT CTCGTTTTGC TGTGATTGAA CGTGCCAGCA TTGATGAGGC TTACGTAGAT
CTGACCAGTG CTGTACAAGA GAGACTACAA AAGCTACAAG GTCAGCCTAT CTCGGCAGAC
TTGTTGCCAA GCACTTACAT TGAAGGGTTG CCCCAAGGCC CTACAACGGC AGAAGAGACT
GTTCAGAAAG AGGGGATGCG AAAACAAGGC TTATTTCAAT GGCTCGATTC TCTTCAGATT
GATAACCTCA CCTCTCCAGA CCTGCAGCTC ACCGTGGGAG CAGTGATTGT GGAGGAAATG
AGAGCAGCCA TAGAGAGGGA GACTGGTTTT CAGTGTTCAG CTGGAATTTC ACACAATAAG
GTCCTGGCAA AACTGGCCTG TGGACTAAAC AAGCCCAACC GCCAAACCCT GGTTTCACAT
GGGTCAGTCC CACAGCTCTT CAGCCAAATG CCCATTCGCA AAATCCGTAG TCTTGGAGGA
AAGCTAGGGG CCTCTGTCAT TGAGATCCTA GGGATAGAAT ACATGGGTGA ACTGACCCAG
TTCACTGAAT CCCAGCTCCA GAGTCATTTT GGGGAGAAGA ATGGGTCTTG GCTATATGCC
ATGTGCCGAG GGATTGAACA TGATCCAGTT AAACCCAGGC AACTACCCAA AACCATTGGC
TGTAGTAAGA ACTTCCCAGG AAAAACAGCT CTTGCTACTC GGGAACAGGT ACAATGGTGG
CTGTTGCAAT TAGCCCAGGA ACTAGAGGAG AGACTGACTA AAGACCGAAA TGATAATGAC
AGGGTAGCCA CCCAGCTGGT TGTGAGCATT CGTGTACAAG GAGACAAACG CCTCAGCAGC
CTGCGCCGCT GCTGTGCCCT TACCCGCTAT GATGCTCACA AGATGAGCCA TGATGCATTT
ACTGTCATCA AGAACTGTAA TACTTCTGGA ATCCAGACAG AATGGTCTCC TCCTCTCACA
ATGCTTTTCC TCTGTGCTAC AAAATTTTCT GCCTCTGCCC CTTCATCTTC TACAGACATC
ACCAGCTTCT TGAGCAGTGA CCCAAGTTCT CTGCCAAAGG TGCCAGTTAC CAGCTCAGAA
GCTAAGACCC AGGGAAGTGG CCCAGCGGTG ACAGCCACTA AGAAAGCAAC CACGTCTCTG
GAATCATTCT TCCAAAAAGC TGCAGAAAGG CAGAAAGTTA AAGAAGCTTC GCTTTCATCT
CTTACTGCTC CCACTCAGGC TCCCATGAGC AATTCACCAT CCAAGCCCTC ATTACCTTTT
CAAACCAGTC AAAGTACAGG AACTGAGCCC TTCTTTAAGC AGAAAAGTCT GCTTCTAAAG
CAGAAACAGC TTAATAATTC TTCAGTTTCT TCCCCCCAAC AAAACCCATG GTCCAACTGT
AAAGCATTAC CAAACTCTTT ACCAACAGAG TATCCAGGGT GTGTCCCTGT TTGTGAAGGG
GTGTCGAAGC TAGAAGAATC CTCTAAAGCA ACTCCTGCAG AGATGGATTT GGCCCACAAC
AGCCAAAGCA TGCACGCCTC TTCAGCTTCC AAATCTGTGC TGGAGGTGAC TCAGAAAGCA
ACCCCAAATC CAAGTCTTCT AGCTGCTGAG GACCAAGTGC CCTGTGAGAA GTGTGGCTCC
CTGGTACCGG TATGGGATAT GCCAGAACAC ATGGACTATC ATTTTGCATT GGAGTTGCAG
AAATCCTTTT TGCAGCCCCA CTCTTCAAAC CCCCAGGTTG TTTCTGCCGT ATCTCATCAA
GGCAAAAGAA ATCCCAAGAG CCCTTTGGCC TGCACTAATA AACGCCCCAG GCCTGAGGGC
ATGCAAACAT TGGAATCATT TTTTAAGCCA TTAACACATT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_006493.1
CDS305..2446
Misc Feature(1)299..301(+)
Misc Feature(2)323..>1366(+)
Misc Feature(3)332..1588(+)
Misc Feature(4)341..997(+)
Misc Feature(5)416..1450(+)
Misc Feature(6)1439..1441(+)
Misc Feature(7)1442..1444(+)
Exon (1)1..300
Gene:POLH
Gene Synonym:
Exon (2)301..441
Gene:POLH
Gene Synonym:
Exon (3)442..576
Gene:POLH
Gene Synonym:
Exon (4)577..794
Gene:POLH
Gene Synonym:
Exon (5)795..964
Gene:POLH
Gene Synonym:
Exon (6)965..1068
Gene:POLH
Gene Synonym:
Exon (7)1069..1188
Gene:POLH
Gene Synonym:
Exon (8)1189..1312
Gene:POLH
Gene Synonym:
Exon (9)1313..1378
Gene:POLH
Gene Synonym:
Exon (10)1379..1548
Gene:POLH
Gene Synonym:
Exon (11)1549..8412
Gene:POLH
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_006502
Organism Homo sapiens (human)
Definition Homo sapiens polymerase (DNA directed), eta (POLH), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_006502

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGGCTACTG GACAGGATCG AGTGGTTGCT CTCGTGGACA TGGACTGTTT TTTTGTTCAA 
GTGGAGCAGC GGCAAAATCC TCATTTGAGG AATAAACCTT GTGCAGTTGT ACAGTACAAA
TCATGGAAGG GTGGTGGAAT AATTGCAGTG AGTTATGAAG CTCGTGCATT TGGAGTCACT
AGAAGTATGT GGGCAGATGA TGCTAAGAAG TTATGTCCAG ATCTTCTACT GGCACAAGTT
CGTGAGTCCC GTGGGAAAGC TAACCTCACC AAGTACCGGG AAGCCAGTGT TGAAGTGATG
GAGATAATGT CTCGTTTTGC TGTGATTGAA CGTGCCAGCA TTGATGAGGC TTACGTAGAT
CTGACCAGTG CTGTACAAGA GAGACTACAA AAGCTACAAG GTCAGCCTAT CTCGGCAGAC
TTGTTGCCAA GCACTTACAT TGAAGGGTTG CCCCAAGGCC CTACAACGGC AGAAGAGACT
GTTCAGAAAG AGGGGATGCG AAAACAAGGC TTATTTCAAT GGCTCGATTC TCTTCAGATT
GATAACCTCA CCTCTCCAGA CCTGCAGCTC ACCGTGGGAG CAGTGATTGT GGAGGAAATG
AGAGCAGCCA TAGAGAGGGA GACTGGTTTT CAGTGTTCAG CTGGAATTTC ACACAATAAG
GTCCTGGCAA AACTGGCCTG TGGACTAAAC AAGCCCAACC GCCAAACCCT GGTTTCACAT
GGGTCAGTCC CACAGCTCTT CAGCCAAATG CCCATTCGCA AAATCCGTAG TCTTGGAGGA
AAGCTAGGGG CCTCTGTCAT TGAGATCCTA GGGATAGAAT ACATGGGTGA ACTGACCCAG
TTCACTGAAT CCCAGCTCCA GAGTCATTTT GGGGAGAAGA ATGGGTCTTG GCTATATGCC
ATGTGCCGAG GGATTGAACA TGATCCAGTT AAACCCAGGC AACTACCCAA AACCATTGGC
TGTAGTAAGA ACTTCCCAGG AAAAACAGCT CTTGCTACTC GGGAACAGGT ACAATGGTGG
CTGTTGCAAT TAGCCCAGGA ACTAGAGGAG AGACTGACTA AAGACCGAAA TGATAATGAC
AGGGTAGCCA CCCAGCTGGT TGTGAGCATT CGTGTACAAG GAGACAAACG CCTCAGCAGC
CTGCGCCGCT GCTGTGCCCT TACCCGCTAT GATGCTCACA AGATGAGCCA TGATGCATTT
ACTGTCATCA AGAACTGTAA TACTTCTGGA ATCCAGACAG AATGGTCTCC TCCTCTCACA
ATGCTTTTCC TCTGTGCTAC AAAATTTTCT GCCTCTGCCC CTTCATCTTC TACAGACATC
ACCAGCTTCT TGAGCAGTGA CCCAAGTTCT CTGCCAAAGG TGCCAGTTAC CAGCTCAGAA
GCTAAGACCC AGGGAAGTGG CCCAGCGGTG ACAGCCACTA AGAAAGCAAC CACGTCTCTG
GAATCATTCT TCCAAAAAGC TGCAGAAAGG CAGAAAGTTA AAGAAGCTTC GCTTTCATCT
CTTACTGCTC CCACTCAGGC TCCCATGAGC AATTCACCAT CCAAGCCCTC ATTACCTTTT
CAAACCAGTC AAAGTACAGG AACTGAGCCC TTCTTTAAGC AGAAAAGTCT GCTTCTAAAG
CAGAAACAGC TTAATAATTC TTCAGTTTCT TCCCCCCAAC AAAACCCATG GTCCAACTGT
AAAGCATTAC CAAACTCTTT ACCAACAGAG TATCCAGGGT GTGTCCCTGT TTGTGAAGGG
GTGTCGAAGC TAGAAGAATC CTCTAAAGCA ACTCCTGCAG AGATGGATTT GGCCCACAAC
AGCCAAAGCA TGCACGCCTC TTCAGCTTCC AAATCTGTGC TGGAGGTGAC TCAGAAAGCA
ACCCCAAATC CAAGTCTTCT AGCTGCTGAG GACCAAGTGC CCTGTGAGAA GTGTGGCTCC
CTGGTACCGG TATGGGATAT GCCAGAACAC ATGGACTATC ATTTTGCATT GGAGTTGCAG
AAATCCTTTT TGCAGCCCCA CTCTTCAAAC CCCCAGGTTG TTTCTGCCGT ATCTCATCAA
GGCAAAAGAA ATCCCAAGAG CCCTTTGGCC TGCACTAATA AACGCCCCAG GCCTGAGGGC
ATGCAAACAT TGGAATCATT TTTTAAGCCA TTAACACATT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24571
Clone ID Related Accession (Same CDS sequence) NM_001291969 , XM_011514698
Accession Version NM_001291969.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1770bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product DNA polymerase eta isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC416114.1, AK307660.1, AK023893.1 and AL353602.10. Transcript Variant: This variant (2) lacks two alternate exons compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK307660.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGAAGCTCG TGCATTTGGA GTCACTAGAA GTATGTGGGC AGATGATGCT AAGAAGTTAT 
GTCCAGATCT TCTACTGGCA CAAGTTCGTG AGTCCCGTGG GAAAGCTAAC CTCACCAAAG
GGGATGCGAA AACAAGGCTT ATTTCAATGG CTCGATTCTC TTCAGATTGA TAACCTCACC
TCTCCAGACC TGCAGCTCAC CGTGGGAGCA GTGATTGTGG AGGAAATGAG AGCAGCCATA
GAGAGGGAGA CTGGTTTTCA GTGTTCAGCT GGAATTTCAC ACAATAAGGT CCTGGCAAAA
CTGGCCTGTG GACTAAACAA GCCCAACCGC CAAACCCTGG TTTCACATGG GTCAGTCCCA
CAGCTCTTCA GCCAAATGCC CATTCGCAAA ATCCGTAGTC TTGGAGGAAA GCTAGGGGCC
TCTGTCATTG AGATCCTAGG GATAGAATAC ATGGGTGAAC TGACCCAGTT CACTGAATCC
CAGCTCCAGA GTCATTTTGG GGAGAAGAAT GGGTCTTGGC TATATGCCAT GTGCCGAGGG
ATTGAACATG ATCCAGTTAA ACCCAGGCAA CTACCCAAAA CCATTGGCTG TAGTAAGAAC
TTCCCAGGAA AAACAGCTCT TGCTACTCGG GAACAGGTAC AATGGTGGCT GTTGCAATTA
GCCCAGGAAC TAGAGGAGAG ACTGACTAAA GACCGAAATG ATAATGACAG GGTAGCCACC
CAGCTGGTTG TGAGCATTCG TGTACAAGGA GACAAACGCC TCAGCAGCCT GCGCCGCTGC
TGTGCCCTTA CCCGCTATGA TGCTCACAAG ATGAGCCATG ATGCATTTAC TGTCATCAAG
AACTGTAATA CTTCTGGAAT CCAGACAGAA TGGTCTCCTC CTCTCACAAT GCTTTTCCTC
TGTGCTACAA AATTTTCTGC CTCTGCCCCT TCATCTTCTA CAGACATCAC CAGCTTCTTG
AGCAGTGACC CAAGTTCTCT GCCAAAGGTG CCAGTTACCA GCTCAGAAGC TAAGACCCAG
GGAAGTGGCC CAGCGGTGAC AGCCACTAAG AAAGCAACCA CGTCTCTGGA ATCATTCTTC
CAAAAAGCTG CAGAAAGGCA GAAAGTTAAA GAAGCTTCGC TTTCATCTCT TACTGCTCCC
ACTCAGGCTC CCATGAGCAA TTCACCATCC AAGCCCTCAT TACCTTTTCA AACCAGTCAA
AGTACAGGAA CTGAGCCCTT CTTTAAGCAG AAAAGTCTGC TTCTAAAGCA GAAACAGCTT
AATAATTCTT CAGTTTCTTC CCCCCAACAA AACCCATGGT CCAACTGTAA AGCATTACCA
AACTCTTTAC CAACAGAGTA TCCAGGGTGT GTCCCTGTTT GTGAAGGGGT GTCGAAGCTA
GAAGAATCCT CTAAAGCAAC TCCTGCAGAG ATGGATTTGG CCCACAACAG CCAAAGCATG
CACGCCTCTT CAGCTTCCAA ATCTGTGCTG GAGGTGACTC AGAAAGCAAC CCCAAATCCA
AGTCTTCTAG CTGCTGAGGA CCAAGTGCCC TGTGAGAAGT GTGGCTCCCT GGTACCGGTA
TGGGATATGC CAGAACACAT GGACTATCAT TTTGCATTGG AGTTGCAGAA ATCCTTTTTG
CAGCCCCACT CTTCAAACCC CCAGGTTGTT TCTGCCGTAT CTCATCAAGG CAAAAGAAAT
CCCAAGAGCC CTTTGGCCTG CACTAATAAA CGCCCCAGGC CTGAGGGCAT GCAAACATTG
GAATCATTTT TTAAGCCATT AACACATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001278898.1
CDS318..2087
Misc Feature(1)312..314(+)
Misc Feature(2)513..1229(+)
Misc Feature(3)711..1103(+)
Exon (1)1..300
Gene:POLH
Gene Synonym:
Exon (2)301..435
Gene:POLH
Gene Synonym:
Exon (3)436..605
Gene:POLH
Gene Synonym:
Exon (4)606..709
Gene:POLH
Gene Synonym:
Exon (5)710..829
Gene:POLH
Gene Synonym:
Exon (6)830..953
Gene:POLH
Gene Synonym:
Exon (7)954..1019
Gene:POLH
Gene Synonym:
Exon (8)1020..1189
Gene:POLH
Gene Synonym:
Exon (9)1190..8053
Gene:POLH
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001291969
Organism Homo sapiens (human)
Definition Homo sapiens polymerase (DNA directed), eta (POLH), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001291969

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGAAGCTCG TGCATTTGGA GTCACTAGAA GTATGTGGGC AGATGATGCT AAGAAGTTAT 
GTCCAGATCT TCTACTGGCA CAAGTTCGTG AGTCCCGTGG GAAAGCTAAC CTCACCAAAG
GGGATGCGAA AACAAGGCTT ATTTCAATGG CTCGATTCTC TTCAGATTGA TAACCTCACC
TCTCCAGACC TGCAGCTCAC CGTGGGAGCA GTGATTGTGG AGGAAATGAG AGCAGCCATA
GAGAGGGAGA CTGGTTTTCA GTGTTCAGCT GGAATTTCAC ACAATAAGGT CCTGGCAAAA
CTGGCCTGTG GACTAAACAA GCCCAACCGC CAAACCCTGG TTTCACATGG GTCAGTCCCA
CAGCTCTTCA GCCAAATGCC CATTCGCAAA ATCCGTAGTC TTGGAGGAAA GCTAGGGGCC
TCTGTCATTG AGATCCTAGG GATAGAATAC ATGGGTGAAC TGACCCAGTT CACTGAATCC
CAGCTCCAGA GTCATTTTGG GGAGAAGAAT GGGTCTTGGC TATATGCCAT GTGCCGAGGG
ATTGAACATG ATCCAGTTAA ACCCAGGCAA CTACCCAAAA CCATTGGCTG TAGTAAGAAC
TTCCCAGGAA AAACAGCTCT TGCTACTCGG GAACAGGTAC AATGGTGGCT GTTGCAATTA
GCCCAGGAAC TAGAGGAGAG ACTGACTAAA GACCGAAATG ATAATGACAG GGTAGCCACC
CAGCTGGTTG TGAGCATTCG TGTACAAGGA GACAAACGCC TCAGCAGCCT GCGCCGCTGC
TGTGCCCTTA CCCGCTATGA TGCTCACAAG ATGAGCCATG ATGCATTTAC TGTCATCAAG
AACTGTAATA CTTCTGGAAT CCAGACAGAA TGGTCTCCTC CTCTCACAAT GCTTTTCCTC
TGTGCTACAA AATTTTCTGC CTCTGCCCCT TCATCTTCTA CAGACATCAC CAGCTTCTTG
AGCAGTGACC CAAGTTCTCT GCCAAAGGTG CCAGTTACCA GCTCAGAAGC TAAGACCCAG
GGAAGTGGCC CAGCGGTGAC AGCCACTAAG AAAGCAACCA CGTCTCTGGA ATCATTCTTC
CAAAAAGCTG CAGAAAGGCA GAAAGTTAAA GAAGCTTCGC TTTCATCTCT TACTGCTCCC
ACTCAGGCTC CCATGAGCAA TTCACCATCC AAGCCCTCAT TACCTTTTCA AACCAGTCAA
AGTACAGGAA CTGAGCCCTT CTTTAAGCAG AAAAGTCTGC TTCTAAAGCA GAAACAGCTT
AATAATTCTT CAGTTTCTTC CCCCCAACAA AACCCATGGT CCAACTGTAA AGCATTACCA
AACTCTTTAC CAACAGAGTA TCCAGGGTGT GTCCCTGTTT GTGAAGGGGT GTCGAAGCTA
GAAGAATCCT CTAAAGCAAC TCCTGCAGAG ATGGATTTGG CCCACAACAG CCAAAGCATG
CACGCCTCTT CAGCTTCCAA ATCTGTGCTG GAGGTGACTC AGAAAGCAAC CCCAAATCCA
AGTCTTCTAG CTGCTGAGGA CCAAGTGCCC TGTGAGAAGT GTGGCTCCCT GGTACCGGTA
TGGGATATGC CAGAACACAT GGACTATCAT TTTGCATTGG AGTTGCAGAA ATCCTTTTTG
CAGCCCCACT CTTCAAACCC CCAGGTTGTT TCTGCCGTAT CTCATCAAGG CAAAAGAAAT
CCCAAGAGCC CTTTGGCCTG CACTAATAAA CGCCCCAGGC CTGAGGGCAT GCAAACATTG
GAATCATTTT TTAAGCCATT AACACATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24572
Clone ID Related Accession (Same CDS sequence) NM_001291970
Accession Version NM_001291970.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1245bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 13-MAY-2015
Organism Homo sapiens (human)
Product DNA polymerase eta isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC416114.1, BC015742.1 and AL353602.10. Transcript Variant: This variant (3) uses an alternate splice junction at the 3' end of an exon compared to variant 1. The resulting isoform (3) is shorter at the C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC015742.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGCTACTG GACAGGATCG AGTGGTTGCT CTCGTGGACA TGGACTGTTT TTTTGTTCAA 
GTGGAGCAGC GGCAAAATCC TCATTTGAGG AATAAACCTT GTGCAGTTGT ACAGTACAAA
TCATGGAAGG GTGGTGGAAT AATTGCAGTG AGTTATGAAG CTCGTGCATT TGGAGTCACT
AGAAGTATGT GGGCAGATGA TGCTAAGAAG TTATGTCCAG ATCTTCTACT GGCACAAGTT
CGTGAGTCCC GTGGGAAAGC TAACCTCACC AAGTACCGGG AAGCCAGTGT TGAAGTGATG
GAGATAATGT CTCGTTTTGC TGTGATTGAA CGTGCCAGCA TTGATGAGGC TTACGTAGAT
CTGACCAGTG CTGTACAAGA GAGACTACAA AAGCTACAAG GTCAGCCTAT CTCGGCAGAC
TTGTTGCCAA GCACTTACAT TGAAGGGTTG CCCCAAGGCC CTACAACGGC AGAAGAGACT
GTTCAGAAAG AGGGGATGCG AAAACAAGGC TTATTTCAAT GGCTCGATTC TCTTCAGATT
GATAACCTCA CCTCTCCAGA CCTGCAGCTC ACCGTGGGAG CAGTGATTGT GGAGGAAATG
AGAGCAGCCA TAGAGAGGGA GACTGGTTTT CAGTGTTCAG CTGGAATTTC ACACAATAAG
GTCCTGGCAA AACTGGCCTG TGGACTAAAC AAGCCCAACC GCCAAACCCT GGTTTCACAT
GGGTCAGTCC CACAGCTCTT CAGCCAAATG CCCATTCGCA AAATCCGTAG TCTTGGAGGA
AAGCTAGGGG CCTCTGTCAT TGAGATCCTA GGGATAGAAT ACATGGGTGA ACTGACCCAG
TTCACTGAAT CCCAGCTCCA GAGTCATTTT GGGGAGAAGA ATGGGTCTTG GCTATATGCC
ATGTGCCGAG GGATTGAACA TGATCCAGTT AAACCCAGGC AACTACCCAA AACCATTGGC
TGTAGTAAGA ACTTCCCAGG AAAAACAGCT CTTGCTACTC GGGAACAGGT ACAATGGTGG
CTGTTGCAAT TAGCCCAGGA ACTAGAGGAG AGACTGACTA AAGACCGAAA TGATAATGAC
AGGGTAGCCA CCCAGCTGGT TGTGAGCATT CGTGTACAAG GAGACAAACG CCTCAGCAGC
CTGCGCCGCT GCTGTGCCCT TACCCGCTAT GATGCTCACA AGATGAGCCA TGATGCATTT
ACTGTCATCA AGAACTGTAA TACTTCTGGA ATCCAGACAG AATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001278899.1
CDS305..1549
Misc Feature(1)299..301(+)
Misc Feature(2)323..>1366(+)
Misc Feature(3)332..1546(+)
Misc Feature(4)341..997(+)
Misc Feature(5)416..1450(+)
Exon (1)1..300
Gene:POLH
Gene Synonym:
Exon (2)301..441
Gene:POLH
Gene Synonym:
Exon (3)442..576
Gene:POLH
Gene Synonym:
Exon (4)577..794
Gene:POLH
Gene Synonym:
Exon (5)795..964
Gene:POLH
Gene Synonym:
Exon (6)965..1068
Gene:POLH
Gene Synonym:
Exon (7)1069..1188
Gene:POLH
Gene Synonym:
Exon (8)1189..1312
Gene:POLH
Gene Synonym:
Exon (9)1313..1378
Gene:POLH
Gene Synonym:
Exon (10)1379..1548
Gene:POLH
Gene Synonym:
Exon (11)1549..8341
Gene:POLH
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001291970
Organism Homo sapiens (human)
Definition Homo sapiens polymerase (DNA directed), eta (POLH), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001291970

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGCTACTG GACAGGATCG AGTGGTTGCT CTCGTGGACA TGGACTGTTT TTTTGTTCAA 
GTGGAGCAGC GGCAAAATCC TCATTTGAGG AATAAACCTT GTGCAGTTGT ACAGTACAAA
TCATGGAAGG GTGGTGGAAT AATTGCAGTG AGTTATGAAG CTCGTGCATT TGGAGTCACT
AGAAGTATGT GGGCAGATGA TGCTAAGAAG TTATGTCCAG ATCTTCTACT GGCACAAGTT
CGTGAGTCCC GTGGGAAAGC TAACCTCACC AAGTACCGGG AAGCCAGTGT TGAAGTGATG
GAGATAATGT CTCGTTTTGC TGTGATTGAA CGTGCCAGCA TTGATGAGGC TTACGTAGAT
CTGACCAGTG CTGTACAAGA GAGACTACAA AAGCTACAAG GTCAGCCTAT CTCGGCAGAC
TTGTTGCCAA GCACTTACAT TGAAGGGTTG CCCCAAGGCC CTACAACGGC AGAAGAGACT
GTTCAGAAAG AGGGGATGCG AAAACAAGGC TTATTTCAAT GGCTCGATTC TCTTCAGATT
GATAACCTCA CCTCTCCAGA CCTGCAGCTC ACCGTGGGAG CAGTGATTGT GGAGGAAATG
AGAGCAGCCA TAGAGAGGGA GACTGGTTTT CAGTGTTCAG CTGGAATTTC ACACAATAAG
GTCCTGGCAA AACTGGCCTG TGGACTAAAC AAGCCCAACC GCCAAACCCT GGTTTCACAT
GGGTCAGTCC CACAGCTCTT CAGCCAAATG CCCATTCGCA AAATCCGTAG TCTTGGAGGA
AAGCTAGGGG CCTCTGTCAT TGAGATCCTA GGGATAGAAT ACATGGGTGA ACTGACCCAG
TTCACTGAAT CCCAGCTCCA GAGTCATTTT GGGGAGAAGA ATGGGTCTTG GCTATATGCC
ATGTGCCGAG GGATTGAACA TGATCCAGTT AAACCCAGGC AACTACCCAA AACCATTGGC
TGTAGTAAGA ACTTCCCAGG AAAAACAGCT CTTGCTACTC GGGAACAGGT ACAATGGTGG
CTGTTGCAAT TAGCCCAGGA ACTAGAGGAG AGACTGACTA AAGACCGAAA TGATAATGAC
AGGGTAGCCA CCCAGCTGGT TGTGAGCATT CGTGTACAAG GAGACAAACG CCTCAGCAGC
CTGCGCCGCT GCTGTGCCCT TACCCGCTAT GATGCTCACA AGATGAGCCA TGATGCATTT
ACTGTCATCA AGAACTGTAA TACTTCTGGA ATCCAGACAG AATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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