ASNSD1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Gene Symbol	ASNSD1
Entrez Gene ID	54529
Full Name	asparagine synthetase domain containing 1
Synonyms	NBLA00058,NS3TP1
General protein information	Preferred Names asparagine synthetase domain containing 1 Names asparagine synthetase domain-containing protein 1 HCV NS3-transactivated protein 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q32.2

mRNA and Protein(s)

mRNA	Protein	Name
NM_019048.4	NP_061921.2	asparagine synthetase domain-containing protein 1
XM_017004381.1	XP_016859870.1	asparagine synthetase domain-containing protein 1 isoform X1
NM_001353498.1	NP_001340427.1	asparagine synthetase domain-containing protein 1
NM_019048.3	NP_061921.2	asparagine synthetase domain-containing protein 1
XM_017004382.1	XP_016859871.1	asparagine synthetase domain-containing protein 1 isoform X1
NM_019048.2	NP_061921.1	asparagine synthetase domain-containing protein 1
NM_001353497.1	NP_001340426.1	asparagine synthetase domain-containing protein 1

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	YML096W	NP_013613.1
Magnaporthe oryzae (rice blast fungus)	MGG_05371	XP_003710217.1
Arabidopsis thaliana (thale cress)	AT2G03667	NP_001077875.2
Xenopus tropicalis (tropical clawed frog)	asnsd1	XP_002931571.1
Mus musculus (house mouse)	Asnsd1	NP_598489.2
	ASNSD1	XP_001106941.1
Kluyveromyces lactis	KLLA0D10285g	XP_453519.1
Oryza sativa (rice)	Os12g0574900	NP_001067104.1
Pan troglodytes (chimpanzee)	ASNSD1	XP_515983.3
Rattus norvegicus (Norway rat)	Asnsd1	NP_001100233.1
Danio rerio (zebrafish)	asnsd1	NP_956389.1
Schizosaccharomyces pombe (fission yeast)	SPBC4F6.11c	NP_596111.1
Homo sapiens (human)	ASNSD1	NP_061921.1
Canis lupus familiaris (dog)	ASNSD1	XP_005640497.1
Bos taurus (cattle)	ASNSD1	NP_001029852.1
Gallus gallus (chicken)	ASNSD1	NP_001006509.1
Drosophila melanogaster (fruit fly)	CG17486	NP_001036446.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010492	XP_001237560.2
Caenorhabditis elegans (roundworm)	M18.3	NP_502296.1
Eremothecium gossypii	AGOS_ADL350C	NP_983745.2
Neurospora crassa	NCU07300	XP_962154.1

Gene Ontology
Bibliography

Related articles in PubMed

Construction of eukaryotic expression plasmid containing HCV NS3 segment and protein expression in human HL-7702 hepatocytes.
Li JW, Li K, Jiang J, Xu XL, Huang ZQ
World journal of gastroenterology12(7)1038-42(2006 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Normal human HL-7702 hepatocytes can efficiently express specific HCV NS3 protein in vitro.
Title: Construction of eukaryotic expression plasmid containing HCV NS3 segment and protein expression in human HL-7702 hepatocytes.

The following ASNSD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ASNSD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31431	NM_019048.2 Latest version!	Homo sapiens asparagine synthetase domain containing 1 (ASNSD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu36369	NM_001353498.1 Latest version!	Homo sapiens asparagine synthetase domain containing 1 (ASNSD1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu36369	NM_019048.4 Latest version!	Homo sapiens asparagine synthetase domain containing 1 (ASNSD1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu36369	NM_001353497.1 Latest version!	Homo sapiens asparagine synthetase domain containing 1 (ASNSD1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu36369	XM_017004381.1 Latest version!	Homo sapiens asparagine synthetase domain containing 1 (ASNSD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu36369	XM_017004382.1 Latest version!	Homo sapiens asparagine synthetase domain containing 1 (ASNSD1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
View Old Accession Versions >>
OHu36369	NM_019048.3	Homo sapiens asparagine synthetase domain containing 1 (ASNSD1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ASNSD1 ( NM_019048.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_061921.1 Homo sapiens asparagine synthetase domain containing 1 (ASNSD1), mRNA.

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CloneID	OHu31431
Clone ID Related Accession (Same CDS sequence)	NM_019048.2
Accession Version	NM_019048.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-05
Organism	Homo sapiens(Human)
Product	asparagine synthetase domain-containing protein 1
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA771653.1, AK000759.1 and BC001243.1. On Mar 6, 2009 this sequence version replaced NM_019048.1. ##Evidence-Data-START## Transcript exon combination :: BC001243.1, SRR1660807.143569.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGTGGCA TTTGTTGTTC TGTAAACTTT TCTGCTGAGC ATTTCAGTCA AGATTTAAAA 
GAGGACTTAC TATATAATCT TAAACAGCGG GGACCCAATA GTAGTAAACA ATTGTTAAAG 
TCTGATGTTA ACTACCAGTG TTTATTTTCT GCTCACGTCC TACACTTGAG GGGTGTTTTG 
ACTACCCAGC CTGTGGAAGA TGAAAGAGGC AATGTGTTTC TATGGAATGG AGAAATTTTT 
AGTGGAATAA AGGTTGAAGC TGAAGAGAAT GACACTCAAA TTTTGTTTAA TTATCTTTCC 
TCCTGTAAGA ATGAATCTGA GATTTTGTCA CTCTTCTCAG AAGTACAAGG TCCCTGGTCA 
TTTATATATT ATCAAGCATC TAGTCATTAT TTATGGTTTG GTAGGGATTT TTTTGGTCGC 
CGTAGCTTGC TTTGGCATTT TAGTAATTTG GGCAAGAGTT TCTGCCTCTC TTCAGTTGGC 
ACCCAAACAT CTGGATTGGC AAATCAGTGG CAAGAAGTTC CAGCATCTGG ACTTTTCAGA 
ATTGATCTTA AGTCTACTGT CATTTCCAGA TGCATTATTT TACAACTGTA TCCTTGGAAA 
TATATTTCTA GGGAGAATAT TATTGAAGAA AATGTTAATA GCCTGAGTCA AATTTCAGCA 
GACTTACCAG CATTTGTATC AGTGGTAGCA AATGAAGCCA AACTGTATCT TGAAAAACCT 
GTTGTTCCTT TAAATATGAT GTTGCCACAA GCTGCATTGG AGACTCATTG CAGTAATATT 
TCCAATGTGC CACCTACAAG AGAGATACTT CAAGTCTTTC TTACTGATGT ACACATGAAG 
GAAGTAATTC AGCAGTTCAT TGATGTCCTG AGTGTAGCAG TCAAGAAACG TGTCTTGTGT 
TTACCTAGGG ATGAAAACCT GACAGCAAAT GAAGTTTTGA AAACGTGTGA TAGGAAAGCA 
AATGTTGCAA TCCTGTTTTC TGGGGGCATT GATTCCATGG TTATTGCAAC CCTTGCTGAC 
CGTCATATTC CTTTAGATGA ACCAATTGAT CTTCTTAATG TAGCTTTCAT AGCTGAAGAA 
AAGACCATGC CAACTACCTT TAACAGAGAA GGGAATAAAC AGAAAAATAA ATGTGAAATA 
CCTTCAGAAG AATTCTCTAA AGATGTTGCT GCTGCTGCTG CTGACAGTCC TAATAAACAT 
GTCAGTGTAC CAGATCGAAT CACAGGAAGG GCGGGACTAA AGGAACTACA AGCTGTTAGC 
CCTTCCCGAA TTTGGAATTT TGTTGAAATT AATGTTTCTA TGGAAGAACT GCAGAAATTA 
AGAAGAACTC GAATATGTCA CTTAATTCGG CCATTGGATA CAGTTTTGGA TGATAGCATT 
GGCTGTGCAG TCTGGTTTGC TTCTAGAGGA ATTGGTTGGT TAGTGGCCCA GGAAGGAGTG 
AAATCCTATC AGAGCAATGC AAAGGTAGTT CTCACTGGAA TTGGTGCAGA TGAGCAACTT 
GCAGGTTATT CTCGTCATCG TGTCCGCTTT CAGTCGCATG GGCTGGAAGG ATTGAATAAG 
GAAATAATGA TGGAACTGGG TCGAATTTCT TCTAGAAATC TTGGTCGTGA TGACAGAGTT 
ATTGGTGATC ATGGAAAAGA AGCAAGATTT CCTTTCCTGG ATGAAAATGT TGTCTCCTTT 
CTAAATTCTC TGCCGATTTG GGAAAAAGCA AACTTGACTT TACCCCGAGG AATTGGTGAA 
AAATTACTTT TACGCCTTGC AGCTGTGGAA CTTGGTCTTA CAGCCTCTGC TCTTCTGCCC 
AAACGGGCCA TGCAGTTTGG ATCAAGAATT GCAAAAATGG AAAAAATTAA TGAAAAGGCA 
TCTGATAAAT GTGGACGGCT CCAAATCATG TCCTTAGAAA ATCTTTCTAT TGAAAAGGAG 
ACTAAATTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061921.1
CDS	435..2366
Translation	MCGICCSVNFSAEHFSQDLKEDLLYNLKQRGPNSSKQLLKSDVNYQCLFSAHVLHLRGVLTTQPVEDERGNVFLWNGEIFSGIKVEAEENDTQILFNYLSSCKNESEILSLFSEVQGPWSFIYYQASSHYLWFGRDFFGRRSLLWHFSNLGKSFCLSSVGTQTSGLANQWQEVPASGLFRIDLKSTVISRCIILQLYPWKYISRENIIEENVNSLSQISADLPAFVSVVANEAKLYLEKPVVPLNMMLPQAALETHCSNISNVPPTREILQVFLTDVHMKEVIQQFIDVLSVAVKKRVLCLPRDENLTANEVLKTCDRKANVAILFSGGIDSMVIATLADRHIPLDEPIDLLNVAFIAEEKTMPTTFNREGNKQKNKCEIPSEEFSKDVAAAAADSPNKHVSVPDRITGRAGLKELQAVSPSRIWNFVEINVSMEELQKLRRTRICHLIRPLDTVLDDSIGCAVWFASRGIGWLVAQEGVKSYQSNAKVVLTGIGADEQLAGYSRHRVRFQSHGLEGLNKEIMMELGRISSRNLGRDDRVIGDHGKEARFPFLDENVVSFLNSLPIWEKANLTLPRGIGEKLLLRLAAVELGLTASALLPKRAMQFGSRIAKMEKINEKASDKCGRLQIMSLENLSIEKETKL

Target ORF information:

RefSeq Version	NM_019048.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens asparagine synthetase domain containing 1 (ASNSD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019048.2

ORF Insert Sequence:

ATGTGTGGCA TTTGTTGTTC TGTAAACTTT TCTGCTGAGC ATTTCAGTCA AGATTTAAAA 
GAGGACTTAC TATATAATCT TAAACAGCGG GGACCCAATA GTAGTAAACA ATTGTTAAAG 
TCTGATGTTA ACTACCAGTG TTTATTTTCT GCTCACGTCC TACACTTGAG GGGTGTTTTG 
ACTACCCAGC CTGTGGAAGA TGAAAGAGGC AATGTGTTTC TATGGAATGG AGAAATTTTT 
AGTGGAATAA AGGTTGAAGC TGAAGAGAAT GACACTCAAA TTTTGTTTAA TTATCTTTCC 
TCCTGTAAGA ATGAATCTGA GATTTTGTCA CTCTTCTCAG AAGTACAAGG TCCCTGGTCA 
TTTATATATT ATCAAGCATC TAGTCATTAT TTATGGTTTG GTAGGGATTT TTTTGGTCGC 
CGTAGCTTGC TTTGGCATTT TAGTAATTTG GGCAAGAGTT TCTGCCTCTC TTCAGTTGGC 
ACCCAAACAT CTGGATTGGC AAATCAGTGG CAAGAAGTTC CAGCATCTGG ACTTTTCAGA 
ATTGATCTTA AGTCTACTGT CATTTCCAGA TGCATTATTT TACAACTGTA TCCTTGGAAA 
TATATTTCTA GGGAGAATAT TATTGAAGAA AATGTTAATA GCCTGAGTCA AATTTCAGCA 
GACTTACCAG CATTTGTATC AGTGGTAGCA AATGAAGCCA AACTGTATCT TGAAAAACCT 
GTTGTTCCTT TAAATATGAT GTTGCCACAA GCTGCATTGG AGACTCATTG CAGTAATATT 
TCCAATGTGC CACCTACAAG AGAGATACTT CAAGTCTTTC TTACTGATGT ACACATGAAG 
GAAGTAATTC AGCAGTTCAT TGATGTCCTG AGTGTAGCAG TCAAGAAACG TGTCTTGTGT 
TTACCTAGGG ATGAAAACCT GACAGCAAAT GAAGTTTTGA AAACGTGTGA TAGGAAAGCA 
AATGTTGCAA TCCTGTTTTC TGGGGGCATT GATTCCATGG TTATTGCAAC CCTTGCTGAC 
CGTCATATTC CTTTAGATGA ACCAATTGAT CTTCTTAATG TAGCTTTCAT AGCTGAAGAA 
AAGACCATGC CAACTACCTT TAACAGAGAA GGGAATAAAC AGAAAAATAA ATGTGAAATA 
CCTTCAGAAG AATTCTCTAA AGATGTTGCT GCTGCTGCTG CTGACAGTCC TAATAAACAT 
GTCAGTGTAC CAGATCGAAT CACAGGAAGG GCGGGACTAA AGGAACTACA AGCTGTTAGC 
CCTTCCCGAA TTTGGAATTT TGTTGAAATT AATGTTTCTA TGGAAGAACT GCAGAAATTA 
AGAAGAACTC GAATATGTCA CTTAATTCGG CCATTGGATA CAGTTTTGGA TGATAGCATT 
GGCTGTGCAG TCTGGTTTGC TTCTAGAGGA ATTGGTTGGT TAGTGGCCCA GGAAGGAGTG 
AAATCCTATC AGAGCAATGC AAAGGTAGTT CTCACTGGAA TTGGTGCAGA TGAGCAACTT 
GCAGGTTATT CTCGTCATCG TGTCCGCTTT CAGTCGCATG GGCTGGAAGG ATTGAATAAG 
GAAATAATGA TGGAACTGGG TCGAATTTCT TCTAGAAATC TTGGTCGTGA TGACAGAGTT 
ATTGGTGATC ATGGAAAAGA AGCAAGATTT CCTTTCCTGG ATGAAAATGT TGTCTCCTTT 
CTAAATTCTC TGCCGATTTG GGAAAAAGCA AACTTGACTT TACCCCGAGG AATTGGTGAA 
AAATTACTTT TACGCCTTGC AGCTGTGGAA CTTGGTCTTA CAGCCTCTGC TCTTCTGCCC 
AAACGGGCCA TGCAGTTTGG ATCAAGAATT GCAAAAATGG AAAAAATTAA TGAAAAGGCA 
TCTGATAAAT GTGGACGGCT CCAAATCATG TCCTTAGAAA ATCTTTCTAT TGAAAAGGAG 
ACTAAATTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASNSD1 ( NM_019048.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_061921.2 Homo sapiens asparagine synthetase domain containing 1 (ASNSD1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu36369
Clone ID Related Accession (Same CDS sequence)	NM_019048.4 , XM_017004381.1 , NM_001353498.1 , NM_019048.3 , XM_017004382.1 , NM_001353497.1
Accession Version	NM_019048.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	asparagine synthetase domain-containing protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK000759.1, AC012488.10 and BC001243.1. On Nov 23, 2018 this sequence version replaced NM_019048.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.345878.1, SRR1660803.56066.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000260952.9/ ENSP00000260952.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTGTGGCA TTTGTTGTTC TGTAAACTTT TCTGCTGAGC ATTTCAGTCA AGATTTAAAA 
GAGGACTTAC TATATAATCT TAAACAGCGG GGACCCAATA GTAGTAAACA ATTGTTAAAG 
TCTGATGTTA ACTACCAGTG TTTATTTTCT GCTCACGTCC TACACTTGAG GGGTGTTTTG 
ACTACCCAGC CTGTGGAAGA TGAAAGAGGC AATGTGTTTC TATGGAATGG AGAAATTTTT 
AGTGGAATAA AGGTTGAAGC TGAAGAGAAT GACACTCAAA TTTTGTTTAA TTATCTTTCC 
TCCTGTAAGA ATGAATCTGA GATTTTGTCA CTCTTCTCAG AAGTACAAGG TCCCTGGTCA 
TTTATATATT ATCAAGCATC TAGTCATTAT TTATGGTTTG GTAGGGATTT TTTTGGTCGC 
CGTAGCTTGC TTTGGCATTT TAGTAATTTG GGCAAGAGTT TCTGCCTCTC TTCAGTTGGC 
ACCCAAACAT CTGGATTGGC AAATCAGTGG CAAGAAGTTC CAGCATCTGG ACTTTTCAGA 
ATTGATCTTA AGTCTACTGT CATTTCCGGA TGCATTATTT TACAACTGTA TCCTTGGAAA 
TATATTTCTA GGGAGAATAT TATTGAAGAA AATGTTAATA GCCTGAGTCA AATTTCAGCA 
GACTTACCAG CATTTGTATC AGTGGTAGCA AATGAAGCCA AACTGTATCT TGAAAAACCT 
GTTGTTCCTT TAAATATGAT GTTGCCACAA GCTGCATTGG AGACTCATTG CAGTAATATT 
TCCAATGTGC CACCTACAAG AGAGATACTT CAAGTCTTTC TTACTGATGT ACACATGAAG 
GAAGTAATTC AGCAGTTCAT TGATGTCCTG AGTGTAGCAG TCAAGAAACG TGTCTTGTGT 
TTACCTAGGG ATGAAAACCT GACAGCAAAT GAAGTTTTGA AAACGTGTGA TAGGAAAGCA 
AATGTTGCAA TCCTGTTTTC TGGGGGCATT GATTCCATGG TTATTGCAAC CCTTGCTGAC 
CGTCATATTC CTTTAGATGA ACCAATTGAT CTTCTTAATG TAGCTTTCAT AGCTGAAGAA 
AAGACCATGC CAACTACCTT TAACAGAGAA GGGAATAAAC AGAAAAATAA ATGTGAAATA 
CCTTCAGAAG AATTCTCTAA AGATGTTGCT GCTGCTGCTG CTGACAGTCC TAATAAACAT 
GTCAGTGTAC CAGATCGAAT CACAGGAAGG GCGGGACTAA AGGAACTACA AGCTGTTAGC 
CCTTCCCGAA TTTGGAATTT TGTTGAAATT AATGTTTCTA TGGAAGAACT GCAGAAATTA 
AGAAGAACTC GAATATGTCA CTTAATTCGG CCATTGGATA CAGTTTTGGA TGATAGCATT 
GGCTGTGCAG TCTGGTTTGC TTCTAGAGGA ATTGGTTGGT TAGTGGCCCA GGAAGGAGTG 
AAATCCTATC AGAGCAATGC AAAGGTAGTT CTCACTGGAA TTGGTGCAGA TGAGCAACTT 
GCAGGTTATT CTCGTCATCG TGTCCGCTTT CAGTCGCATG GGCTGGAAGG ATTGAATAAG 
GAAATAATGA TGGAACTGGG TCGAATTTCT TCTAGAAATC TTGGTCGTGA TGACAGAGTT 
ATTGGTGATC ATGGAAAAGA AGCAAGATTT CCTTTCCTGG ATGAAAATGT TGTCTCCTTT 
CTAAATTCTC TGCCGATTTG GGAAAAAGCA AACTTGACTT TACCCCGAGG AATTGGTGAA 
AAATTACTTT TACGCCTTGC AGCTGTGGAA CTTGGTCTTA CAGCCTCTGC TCTTCTGCCC 
AAACGGGCCA TGCAGTTTGG ATCAAGAATT GCAAAAATGG AAAAAATTAA TGAAAAGGCA 
TCTGATAAAT GTGGACGGCT CCAAATCATG TCCTTAGAAA ATCTTTCTAT TGAAAAGGAG 
ACTAAATTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061921.2
CDS	375..2306
Translation	MCGICCSVNFSAEHFSQDLKEDLLYNLKQRGPNSSKQLLKSDVNYQCLFSAHVLHLRGVLTTQPVEDERGNVFLWNGEIFSGIKVEAEENDTQILFNYLSSCKNESEILSLFSEVQGPWSFIYYQASSHYLWFGRDFFGRRSLLWHFSNLGKSFCLSSVGTQTSGLANQWQEVPASGLFRIDLKSTVISGCIILQLYPWKYISRENIIEENVNSLSQISADLPAFVSVVANEAKLYLEKPVVPLNMMLPQAALETHCSNISNVPPTREILQVFLTDVHMKEVIQQFIDVLSVAVKKRVLCLPRDENLTANEVLKTCDRKANVAILFSGGIDSMVIATLADRHIPLDEPIDLLNVAFIAEEKTMPTTFNREGNKQKNKCEIPSEEFSKDVAAAAADSPNKHVSVPDRITGRAGLKELQAVSPSRIWNFVEINVSMEELQKLRRTRICHLIRPLDTVLDDSIGCAVWFASRGIGWLVAQEGVKSYQSNAKVVLTGIGADEQLAGYSRHRVRFQSHGLEGLNKEIMMELGRISSRNLGRDDRVIGDHGKEARFPFLDENVVSFLNSLPIWEKANLTLPRGIGEKLLLRLAAVELGLTASALLPKRAMQFGSRIAKMEKINEKASDKCGRLQIMSLENLSIEKETKL

Target ORF information:

RefSeq Version	NM_019048.4
Organism	Homo sapiens(human)
Definition	Homo sapiens asparagine synthetase domain containing 1 (ASNSD1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019048.4

ORF Insert Sequence:

ATGTGTGGCA TTTGTTGTTC TGTAAACTTT TCTGCTGAGC ATTTCAGTCA AGATTTAAAA 
GAGGACTTAC TATATAATCT TAAACAGCGG GGACCCAATA GTAGTAAACA ATTGTTAAAG 
TCTGATGTTA ACTACCAGTG TTTATTTTCT GCTCACGTCC TACACTTGAG GGGTGTTTTG 
ACTACCCAGC CTGTGGAAGA TGAAAGAGGC AATGTGTTTC TATGGAATGG AGAAATTTTT 
AGTGGAATAA AGGTTGAAGC TGAAGAGAAT GACACTCAAA TTTTGTTTAA TTATCTTTCC 
TCCTGTAAGA ATGAATCTGA GATTTTGTCA CTCTTCTCAG AAGTACAAGG TCCCTGGTCA 
TTTATATATT ATCAAGCATC TAGTCATTAT TTATGGTTTG GTAGGGATTT TTTTGGTCGC 
CGTAGCTTGC TTTGGCATTT TAGTAATTTG GGCAAGAGTT TCTGCCTCTC TTCAGTTGGC 
ACCCAAACAT CTGGATTGGC AAATCAGTGG CAAGAAGTTC CAGCATCTGG ACTTTTCAGA 
ATTGATCTTA AGTCTACTGT CATTTCCGGA TGCATTATTT TACAACTGTA TCCTTGGAAA 
TATATTTCTA GGGAGAATAT TATTGAAGAA AATGTTAATA GCCTGAGTCA AATTTCAGCA 
GACTTACCAG CATTTGTATC AGTGGTAGCA AATGAAGCCA AACTGTATCT TGAAAAACCT 
GTTGTTCCTT TAAATATGAT GTTGCCACAA GCTGCATTGG AGACTCATTG CAGTAATATT 
TCCAATGTGC CACCTACAAG AGAGATACTT CAAGTCTTTC TTACTGATGT ACACATGAAG 
GAAGTAATTC AGCAGTTCAT TGATGTCCTG AGTGTAGCAG TCAAGAAACG TGTCTTGTGT 
TTACCTAGGG ATGAAAACCT GACAGCAAAT GAAGTTTTGA AAACGTGTGA TAGGAAAGCA 
AATGTTGCAA TCCTGTTTTC TGGGGGCATT GATTCCATGG TTATTGCAAC CCTTGCTGAC 
CGTCATATTC CTTTAGATGA ACCAATTGAT CTTCTTAATG TAGCTTTCAT AGCTGAAGAA 
AAGACCATGC CAACTACCTT TAACAGAGAA GGGAATAAAC AGAAAAATAA ATGTGAAATA 
CCTTCAGAAG AATTCTCTAA AGATGTTGCT GCTGCTGCTG CTGACAGTCC TAATAAACAT 
GTCAGTGTAC CAGATCGAAT CACAGGAAGG GCGGGACTAA AGGAACTACA AGCTGTTAGC 
CCTTCCCGAA TTTGGAATTT TGTTGAAATT AATGTTTCTA TGGAAGAACT GCAGAAATTA 
AGAAGAACTC GAATATGTCA CTTAATTCGG CCATTGGATA CAGTTTTGGA TGATAGCATT 
GGCTGTGCAG TCTGGTTTGC TTCTAGAGGA ATTGGTTGGT TAGTGGCCCA GGAAGGAGTG 
AAATCCTATC AGAGCAATGC AAAGGTAGTT CTCACTGGAA TTGGTGCAGA TGAGCAACTT 
GCAGGTTATT CTCGTCATCG TGTCCGCTTT CAGTCGCATG GGCTGGAAGG ATTGAATAAG 
GAAATAATGA TGGAACTGGG TCGAATTTCT TCTAGAAATC TTGGTCGTGA TGACAGAGTT 
ATTGGTGATC ATGGAAAAGA AGCAAGATTT CCTTTCCTGG ATGAAAATGT TGTCTCCTTT 
CTAAATTCTC TGCCGATTTG GGAAAAAGCA AACTTGACTT TACCCCGAGG AATTGGTGAA 
AAATTACTTT TACGCCTTGC AGCTGTGGAA CTTGGTCTTA CAGCCTCTGC TCTTCTGCCC 
AAACGGGCCA TGCAGTTTGG ATCAAGAATT GCAAAAATGG AAAAAATTAA TGAAAAGGCA 
TCTGATAAAT GTGGACGGCT CCAAATCATG TCCTTAGAAA ATCTTTCTAT TGAAAAGGAG 
ACTAAATTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASNSD1 ( NM_001353498.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001340427.1 Homo sapiens asparagine synthetase domain containing 1 (ASNSD1), transcript variant 3, mRNA.

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CloneID	OHu36369
Clone ID Related Accession (Same CDS sequence)	NM_019048.4 , XM_017004381.1 , NM_001353498.1 , NM_019048.3 , XM_017004382.1 , NM_001353497.1
Accession Version	NM_001353498.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-14
Organism	Homo sapiens(human)
Product	asparagine synthetase domain-containing protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC012488.10. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.214380.1, SRR1803617.156500.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2145240 [ECO:0000348] ##Evidence-Data-END##

ATGTGTGGCA TTTGTTGTTC TGTAAACTTT TCTGCTGAGC ATTTCAGTCA AGATTTAAAA 
GAGGACTTAC TATATAATCT TAAACAGCGG GGACCCAATA GTAGTAAACA ATTGTTAAAG 
TCTGATGTTA ACTACCAGTG TTTATTTTCT GCTCACGTCC TACACTTGAG GGGTGTTTTG 
ACTACCCAGC CTGTGGAAGA TGAAAGAGGC AATGTGTTTC TATGGAATGG AGAAATTTTT 
AGTGGAATAA AGGTTGAAGC TGAAGAGAAT GACACTCAAA TTTTGTTTAA TTATCTTTCC 
TCCTGTAAGA ATGAATCTGA GATTTTGTCA CTCTTCTCAG AAGTACAAGG TCCCTGGTCA 
TTTATATATT ATCAAGCATC TAGTCATTAT TTATGGTTTG GTAGGGATTT TTTTGGTCGC 
CGTAGCTTGC TTTGGCATTT TAGTAATTTG GGCAAGAGTT TCTGCCTCTC TTCAGTTGGC 
ACCCAAACAT CTGGATTGGC AAATCAGTGG CAAGAAGTTC CAGCATCTGG ACTTTTCAGA 
ATTGATCTTA AGTCTACTGT CATTTCCGGA TGCATTATTT TACAACTGTA TCCTTGGAAA 
TATATTTCTA GGGAGAATAT TATTGAAGAA AATGTTAATA GCCTGAGTCA AATTTCAGCA 
GACTTACCAG CATTTGTATC AGTGGTAGCA AATGAAGCCA AACTGTATCT TGAAAAACCT 
GTTGTTCCTT TAAATATGAT GTTGCCACAA GCTGCATTGG AGACTCATTG CAGTAATATT 
TCCAATGTGC CACCTACAAG AGAGATACTT CAAGTCTTTC TTACTGATGT ACACATGAAG 
GAAGTAATTC AGCAGTTCAT TGATGTCCTG AGTGTAGCAG TCAAGAAACG TGTCTTGTGT 
TTACCTAGGG ATGAAAACCT GACAGCAAAT GAAGTTTTGA AAACGTGTGA TAGGAAAGCA 
AATGTTGCAA TCCTGTTTTC TGGGGGCATT GATTCCATGG TTATTGCAAC CCTTGCTGAC 
CGTCATATTC CTTTAGATGA ACCAATTGAT CTTCTTAATG TAGCTTTCAT AGCTGAAGAA 
AAGACCATGC CAACTACCTT TAACAGAGAA GGGAATAAAC AGAAAAATAA ATGTGAAATA 
CCTTCAGAAG AATTCTCTAA AGATGTTGCT GCTGCTGCTG CTGACAGTCC TAATAAACAT 
GTCAGTGTAC CAGATCGAAT CACAGGAAGG GCGGGACTAA AGGAACTACA AGCTGTTAGC 
CCTTCCCGAA TTTGGAATTT TGTTGAAATT AATGTTTCTA TGGAAGAACT GCAGAAATTA 
AGAAGAACTC GAATATGTCA CTTAATTCGG CCATTGGATA CAGTTTTGGA TGATAGCATT 
GGCTGTGCAG TCTGGTTTGC TTCTAGAGGA ATTGGTTGGT TAGTGGCCCA GGAAGGAGTG 
AAATCCTATC AGAGCAATGC AAAGGTAGTT CTCACTGGAA TTGGTGCAGA TGAGCAACTT 
GCAGGTTATT CTCGTCATCG TGTCCGCTTT CAGTCGCATG GGCTGGAAGG ATTGAATAAG 
GAAATAATGA TGGAACTGGG TCGAATTTCT TCTAGAAATC TTGGTCGTGA TGACAGAGTT 
ATTGGTGATC ATGGAAAAGA AGCAAGATTT CCTTTCCTGG ATGAAAATGT TGTCTCCTTT 
CTAAATTCTC TGCCGATTTG GGAAAAAGCA AACTTGACTT TACCCCGAGG AATTGGTGAA 
AAATTACTTT TACGCCTTGC AGCTGTGGAA CTTGGTCTTA CAGCCTCTGC TCTTCTGCCC 
AAACGGGCCA TGCAGTTTGG ATCAAGAATT GCAAAAATGG AAAAAATTAA TGAAAAGGCA 
TCTGATAAAT GTGGACGGCT CCAAATCATG TCCTTAGAAA ATCTTTCTAT TGAAAAGGAG 
ACTAAATTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001340427.1
CDS	381..2312
Translation	MCGICCSVNFSAEHFSQDLKEDLLYNLKQRGPNSSKQLLKSDVNYQCLFSAHVLHLRGVLTTQPVEDERGNVFLWNGEIFSGIKVEAEENDTQILFNYLSSCKNESEILSLFSEVQGPWSFIYYQASSHYLWFGRDFFGRRSLLWHFSNLGKSFCLSSVGTQTSGLANQWQEVPASGLFRIDLKSTVISGCIILQLYPWKYISRENIIEENVNSLSQISADLPAFVSVVANEAKLYLEKPVVPLNMMLPQAALETHCSNISNVPPTREILQVFLTDVHMKEVIQQFIDVLSVAVKKRVLCLPRDENLTANEVLKTCDRKANVAILFSGGIDSMVIATLADRHIPLDEPIDLLNVAFIAEEKTMPTTFNREGNKQKNKCEIPSEEFSKDVAAAAADSPNKHVSVPDRITGRAGLKELQAVSPSRIWNFVEINVSMEELQKLRRTRICHLIRPLDTVLDDSIGCAVWFASRGIGWLVAQEGVKSYQSNAKVVLTGIGADEQLAGYSRHRVRFQSHGLEGLNKEIMMELGRISSRNLGRDDRVIGDHGKEARFPFLDENVVSFLNSLPIWEKANLTLPRGIGEKLLLRLAAVELGLTASALLPKRAMQFGSRIAKMEKINEKASDKCGRLQIMSLENLSIEKETKL

Target ORF information:

RefSeq Version	NM_001353498.1
Organism	Homo sapiens(human)
Definition	Homo sapiens asparagine synthetase domain containing 1 (ASNSD1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353498.1

ORF Insert Sequence:

ATGTGTGGCA TTTGTTGTTC TGTAAACTTT TCTGCTGAGC ATTTCAGTCA AGATTTAAAA 
GAGGACTTAC TATATAATCT TAAACAGCGG GGACCCAATA GTAGTAAACA ATTGTTAAAG 
TCTGATGTTA ACTACCAGTG TTTATTTTCT GCTCACGTCC TACACTTGAG GGGTGTTTTG 
ACTACCCAGC CTGTGGAAGA TGAAAGAGGC AATGTGTTTC TATGGAATGG AGAAATTTTT 
AGTGGAATAA AGGTTGAAGC TGAAGAGAAT GACACTCAAA TTTTGTTTAA TTATCTTTCC 
TCCTGTAAGA ATGAATCTGA GATTTTGTCA CTCTTCTCAG AAGTACAAGG TCCCTGGTCA 
TTTATATATT ATCAAGCATC TAGTCATTAT TTATGGTTTG GTAGGGATTT TTTTGGTCGC 
CGTAGCTTGC TTTGGCATTT TAGTAATTTG GGCAAGAGTT TCTGCCTCTC TTCAGTTGGC 
ACCCAAACAT CTGGATTGGC AAATCAGTGG CAAGAAGTTC CAGCATCTGG ACTTTTCAGA 
ATTGATCTTA AGTCTACTGT CATTTCCGGA TGCATTATTT TACAACTGTA TCCTTGGAAA 
TATATTTCTA GGGAGAATAT TATTGAAGAA AATGTTAATA GCCTGAGTCA AATTTCAGCA 
GACTTACCAG CATTTGTATC AGTGGTAGCA AATGAAGCCA AACTGTATCT TGAAAAACCT 
GTTGTTCCTT TAAATATGAT GTTGCCACAA GCTGCATTGG AGACTCATTG CAGTAATATT 
TCCAATGTGC CACCTACAAG AGAGATACTT CAAGTCTTTC TTACTGATGT ACACATGAAG 
GAAGTAATTC AGCAGTTCAT TGATGTCCTG AGTGTAGCAG TCAAGAAACG TGTCTTGTGT 
TTACCTAGGG ATGAAAACCT GACAGCAAAT GAAGTTTTGA AAACGTGTGA TAGGAAAGCA 
AATGTTGCAA TCCTGTTTTC TGGGGGCATT GATTCCATGG TTATTGCAAC CCTTGCTGAC 
CGTCATATTC CTTTAGATGA ACCAATTGAT CTTCTTAATG TAGCTTTCAT AGCTGAAGAA 
AAGACCATGC CAACTACCTT TAACAGAGAA GGGAATAAAC AGAAAAATAA ATGTGAAATA 
CCTTCAGAAG AATTCTCTAA AGATGTTGCT GCTGCTGCTG CTGACAGTCC TAATAAACAT 
GTCAGTGTAC CAGATCGAAT CACAGGAAGG GCGGGACTAA AGGAACTACA AGCTGTTAGC 
CCTTCCCGAA TTTGGAATTT TGTTGAAATT AATGTTTCTA TGGAAGAACT GCAGAAATTA 
AGAAGAACTC GAATATGTCA CTTAATTCGG CCATTGGATA CAGTTTTGGA TGATAGCATT 
GGCTGTGCAG TCTGGTTTGC TTCTAGAGGA ATTGGTTGGT TAGTGGCCCA GGAAGGAGTG 
AAATCCTATC AGAGCAATGC AAAGGTAGTT CTCACTGGAA TTGGTGCAGA TGAGCAACTT 
GCAGGTTATT CTCGTCATCG TGTCCGCTTT CAGTCGCATG GGCTGGAAGG ATTGAATAAG 
GAAATAATGA TGGAACTGGG TCGAATTTCT TCTAGAAATC TTGGTCGTGA TGACAGAGTT 
ATTGGTGATC ATGGAAAAGA AGCAAGATTT CCTTTCCTGG ATGAAAATGT TGTCTCCTTT 
CTAAATTCTC TGCCGATTTG GGAAAAAGCA AACTTGACTT TACCCCGAGG AATTGGTGAA 
AAATTACTTT TACGCCTTGC AGCTGTGGAA CTTGGTCTTA CAGCCTCTGC TCTTCTGCCC 
AAACGGGCCA TGCAGTTTGG ATCAAGAATT GCAAAAATGG AAAAAATTAA TGAAAAGGCA 
TCTGATAAAT GTGGACGGCT CCAAATCATG TCCTTAGAAA ATCTTTCTAT TGAAAAGGAG 
ACTAAATTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASNSD1 ( NM_019048.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_061921.2 Homo sapiens asparagine synthetase domain containing 1 (ASNSD1), transcript variant 1, mRNA.

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CloneID	OHu36369
Clone ID Related Accession (Same CDS sequence)	NM_019048.4 , XM_017004381.1 , NM_001353498.1 , NM_019048.3 , XM_017004382.1 , NM_001353497.1
Accession Version	NM_019048.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-09
Organism	Homo sapiens(human)
Product	asparagine synthetase domain-containing protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA771653.1, AK000759.1, AC012488.10 and BC001243.1. On Jul 19, 2017 this sequence version replaced NM_019048.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC001243.1, SRR1660807.143569.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGTGGCA TTTGTTGTTC TGTAAACTTT TCTGCTGAGC ATTTCAGTCA AGATTTAAAA 
GAGGACTTAC TATATAATCT TAAACAGCGG GGACCCAATA GTAGTAAACA ATTGTTAAAG 
TCTGATGTTA ACTACCAGTG TTTATTTTCT GCTCACGTCC TACACTTGAG GGGTGTTTTG 
ACTACCCAGC CTGTGGAAGA TGAAAGAGGC AATGTGTTTC TATGGAATGG AGAAATTTTT 
AGTGGAATAA AGGTTGAAGC TGAAGAGAAT GACACTCAAA TTTTGTTTAA TTATCTTTCC 
TCCTGTAAGA ATGAATCTGA GATTTTGTCA CTCTTCTCAG AAGTACAAGG TCCCTGGTCA 
TTTATATATT ATCAAGCATC TAGTCATTAT TTATGGTTTG GTAGGGATTT TTTTGGTCGC 
CGTAGCTTGC TTTGGCATTT TAGTAATTTG GGCAAGAGTT TCTGCCTCTC TTCAGTTGGC 
ACCCAAACAT CTGGATTGGC AAATCAGTGG CAAGAAGTTC CAGCATCTGG ACTTTTCAGA 
ATTGATCTTA AGTCTACTGT CATTTCCGGA TGCATTATTT TACAACTGTA TCCTTGGAAA 
TATATTTCTA GGGAGAATAT TATTGAAGAA AATGTTAATA GCCTGAGTCA AATTTCAGCA 
GACTTACCAG CATTTGTATC AGTGGTAGCA AATGAAGCCA AACTGTATCT TGAAAAACCT 
GTTGTTCCTT TAAATATGAT GTTGCCACAA GCTGCATTGG AGACTCATTG CAGTAATATT 
TCCAATGTGC CACCTACAAG AGAGATACTT CAAGTCTTTC TTACTGATGT ACACATGAAG 
GAAGTAATTC AGCAGTTCAT TGATGTCCTG AGTGTAGCAG TCAAGAAACG TGTCTTGTGT 
TTACCTAGGG ATGAAAACCT GACAGCAAAT GAAGTTTTGA AAACGTGTGA TAGGAAAGCA 
AATGTTGCAA TCCTGTTTTC TGGGGGCATT GATTCCATGG TTATTGCAAC CCTTGCTGAC 
CGTCATATTC CTTTAGATGA ACCAATTGAT CTTCTTAATG TAGCTTTCAT AGCTGAAGAA 
AAGACCATGC CAACTACCTT TAACAGAGAA GGGAATAAAC AGAAAAATAA ATGTGAAATA 
CCTTCAGAAG AATTCTCTAA AGATGTTGCT GCTGCTGCTG CTGACAGTCC TAATAAACAT 
GTCAGTGTAC CAGATCGAAT CACAGGAAGG GCGGGACTAA AGGAACTACA AGCTGTTAGC 
CCTTCCCGAA TTTGGAATTT TGTTGAAATT AATGTTTCTA TGGAAGAACT GCAGAAATTA 
AGAAGAACTC GAATATGTCA CTTAATTCGG CCATTGGATA CAGTTTTGGA TGATAGCATT 
GGCTGTGCAG TCTGGTTTGC TTCTAGAGGA ATTGGTTGGT TAGTGGCCCA GGAAGGAGTG 
AAATCCTATC AGAGCAATGC AAAGGTAGTT CTCACTGGAA TTGGTGCAGA TGAGCAACTT 
GCAGGTTATT CTCGTCATCG TGTCCGCTTT CAGTCGCATG GGCTGGAAGG ATTGAATAAG 
GAAATAATGA TGGAACTGGG TCGAATTTCT TCTAGAAATC TTGGTCGTGA TGACAGAGTT 
ATTGGTGATC ATGGAAAAGA AGCAAGATTT CCTTTCCTGG ATGAAAATGT TGTCTCCTTT 
CTAAATTCTC TGCCGATTTG GGAAAAAGCA AACTTGACTT TACCCCGAGG AATTGGTGAA 
AAATTACTTT TACGCCTTGC AGCTGTGGAA CTTGGTCTTA CAGCCTCTGC TCTTCTGCCC 
AAACGGGCCA TGCAGTTTGG ATCAAGAATT GCAAAAATGG AAAAAATTAA TGAAAAGGCA 
TCTGATAAAT GTGGACGGCT CCAAATCATG TCCTTAGAAA ATCTTTCTAT TGAAAAGGAG 
ACTAAATTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061921.2
CDS	435..2366
Translation	MCGICCSVNFSAEHFSQDLKEDLLYNLKQRGPNSSKQLLKSDVNYQCLFSAHVLHLRGVLTTQPVEDERGNVFLWNGEIFSGIKVEAEENDTQILFNYLSSCKNESEILSLFSEVQGPWSFIYYQASSHYLWFGRDFFGRRSLLWHFSNLGKSFCLSSVGTQTSGLANQWQEVPASGLFRIDLKSTVISGCIILQLYPWKYISRENIIEENVNSLSQISADLPAFVSVVANEAKLYLEKPVVPLNMMLPQAALETHCSNISNVPPTREILQVFLTDVHMKEVIQQFIDVLSVAVKKRVLCLPRDENLTANEVLKTCDRKANVAILFSGGIDSMVIATLADRHIPLDEPIDLLNVAFIAEEKTMPTTFNREGNKQKNKCEIPSEEFSKDVAAAAADSPNKHVSVPDRITGRAGLKELQAVSPSRIWNFVEINVSMEELQKLRRTRICHLIRPLDTVLDDSIGCAVWFASRGIGWLVAQEGVKSYQSNAKVVLTGIGADEQLAGYSRHRVRFQSHGLEGLNKEIMMELGRISSRNLGRDDRVIGDHGKEARFPFLDENVVSFLNSLPIWEKANLTLPRGIGEKLLLRLAAVELGLTASALLPKRAMQFGSRIAKMEKINEKASDKCGRLQIMSLENLSIEKETKL

Target ORF information:

RefSeq Version	NM_019048.3
Organism	Homo sapiens(human)
Definition	Homo sapiens asparagine synthetase domain containing 1 (ASNSD1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019048.3

ORF Insert Sequence:

ATGTGTGGCA TTTGTTGTTC TGTAAACTTT TCTGCTGAGC ATTTCAGTCA AGATTTAAAA 
GAGGACTTAC TATATAATCT TAAACAGCGG GGACCCAATA GTAGTAAACA ATTGTTAAAG 
TCTGATGTTA ACTACCAGTG TTTATTTTCT GCTCACGTCC TACACTTGAG GGGTGTTTTG 
ACTACCCAGC CTGTGGAAGA TGAAAGAGGC AATGTGTTTC TATGGAATGG AGAAATTTTT 
AGTGGAATAA AGGTTGAAGC TGAAGAGAAT GACACTCAAA TTTTGTTTAA TTATCTTTCC 
TCCTGTAAGA ATGAATCTGA GATTTTGTCA CTCTTCTCAG AAGTACAAGG TCCCTGGTCA 
TTTATATATT ATCAAGCATC TAGTCATTAT TTATGGTTTG GTAGGGATTT TTTTGGTCGC 
CGTAGCTTGC TTTGGCATTT TAGTAATTTG GGCAAGAGTT TCTGCCTCTC TTCAGTTGGC 
ACCCAAACAT CTGGATTGGC AAATCAGTGG CAAGAAGTTC CAGCATCTGG ACTTTTCAGA 
ATTGATCTTA AGTCTACTGT CATTTCCGGA TGCATTATTT TACAACTGTA TCCTTGGAAA 
TATATTTCTA GGGAGAATAT TATTGAAGAA AATGTTAATA GCCTGAGTCA AATTTCAGCA 
GACTTACCAG CATTTGTATC AGTGGTAGCA AATGAAGCCA AACTGTATCT TGAAAAACCT 
GTTGTTCCTT TAAATATGAT GTTGCCACAA GCTGCATTGG AGACTCATTG CAGTAATATT 
TCCAATGTGC CACCTACAAG AGAGATACTT CAAGTCTTTC TTACTGATGT ACACATGAAG 
GAAGTAATTC AGCAGTTCAT TGATGTCCTG AGTGTAGCAG TCAAGAAACG TGTCTTGTGT 
TTACCTAGGG ATGAAAACCT GACAGCAAAT GAAGTTTTGA AAACGTGTGA TAGGAAAGCA 
AATGTTGCAA TCCTGTTTTC TGGGGGCATT GATTCCATGG TTATTGCAAC CCTTGCTGAC 
CGTCATATTC CTTTAGATGA ACCAATTGAT CTTCTTAATG TAGCTTTCAT AGCTGAAGAA 
AAGACCATGC CAACTACCTT TAACAGAGAA GGGAATAAAC AGAAAAATAA ATGTGAAATA 
CCTTCAGAAG AATTCTCTAA AGATGTTGCT GCTGCTGCTG CTGACAGTCC TAATAAACAT 
GTCAGTGTAC CAGATCGAAT CACAGGAAGG GCGGGACTAA AGGAACTACA AGCTGTTAGC 
CCTTCCCGAA TTTGGAATTT TGTTGAAATT AATGTTTCTA TGGAAGAACT GCAGAAATTA 
AGAAGAACTC GAATATGTCA CTTAATTCGG CCATTGGATA CAGTTTTGGA TGATAGCATT 
GGCTGTGCAG TCTGGTTTGC TTCTAGAGGA ATTGGTTGGT TAGTGGCCCA GGAAGGAGTG 
AAATCCTATC AGAGCAATGC AAAGGTAGTT CTCACTGGAA TTGGTGCAGA TGAGCAACTT 
GCAGGTTATT CTCGTCATCG TGTCCGCTTT CAGTCGCATG GGCTGGAAGG ATTGAATAAG 
GAAATAATGA TGGAACTGGG TCGAATTTCT TCTAGAAATC TTGGTCGTGA TGACAGAGTT 
ATTGGTGATC ATGGAAAAGA AGCAAGATTT CCTTTCCTGG ATGAAAATGT TGTCTCCTTT 
CTAAATTCTC TGCCGATTTG GGAAAAAGCA AACTTGACTT TACCCCGAGG AATTGGTGAA 
AAATTACTTT TACGCCTTGC AGCTGTGGAA CTTGGTCTTA CAGCCTCTGC TCTTCTGCCC 
AAACGGGCCA TGCAGTTTGG ATCAAGAATT GCAAAAATGG AAAAAATTAA TGAAAAGGCA 
TCTGATAAAT GTGGACGGCT CCAAATCATG TCCTTAGAAA ATCTTTCTAT TGAAAAGGAG 
ACTAAATTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASNSD1 ( NM_001353497.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001340426.1 Homo sapiens asparagine synthetase domain containing 1 (ASNSD1), transcript variant 2, mRNA.

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CloneID	OHu36369
Clone ID Related Accession (Same CDS sequence)	NM_019048.4 , XM_017004381.1 , NM_001353498.1 , NM_019048.3 , XM_017004382.1 , NM_001353497.1
Accession Version	NM_001353497.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Homo sapiens(human)
Product	asparagine synthetase domain-containing protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC012488.10. On Mar 23, 2018 this sequence version replaced NM_001353498.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.214380.1, SRR1803617.156500.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2145240 [ECO:0000348] ##Evidence-Data-END##

ATGTGTGGCA TTTGTTGTTC TGTAAACTTT TCTGCTGAGC ATTTCAGTCA AGATTTAAAA 
GAGGACTTAC TATATAATCT TAAACAGCGG GGACCCAATA GTAGTAAACA ATTGTTAAAG 
TCTGATGTTA ACTACCAGTG TTTATTTTCT GCTCACGTCC TACACTTGAG GGGTGTTTTG 
ACTACCCAGC CTGTGGAAGA TGAAAGAGGC AATGTGTTTC TATGGAATGG AGAAATTTTT 
AGTGGAATAA AGGTTGAAGC TGAAGAGAAT GACACTCAAA TTTTGTTTAA TTATCTTTCC 
TCCTGTAAGA ATGAATCTGA GATTTTGTCA CTCTTCTCAG AAGTACAAGG TCCCTGGTCA 
TTTATATATT ATCAAGCATC TAGTCATTAT TTATGGTTTG GTAGGGATTT TTTTGGTCGC 
CGTAGCTTGC TTTGGCATTT TAGTAATTTG GGCAAGAGTT TCTGCCTCTC TTCAGTTGGC 
ACCCAAACAT CTGGATTGGC AAATCAGTGG CAAGAAGTTC CAGCATCTGG ACTTTTCAGA 
ATTGATCTTA AGTCTACTGT CATTTCCGGA TGCATTATTT TACAACTGTA TCCTTGGAAA 
TATATTTCTA GGGAGAATAT TATTGAAGAA AATGTTAATA GCCTGAGTCA AATTTCAGCA 
GACTTACCAG CATTTGTATC AGTGGTAGCA AATGAAGCCA AACTGTATCT TGAAAAACCT 
GTTGTTCCTT TAAATATGAT GTTGCCACAA GCTGCATTGG AGACTCATTG CAGTAATATT 
TCCAATGTGC CACCTACAAG AGAGATACTT CAAGTCTTTC TTACTGATGT ACACATGAAG 
GAAGTAATTC AGCAGTTCAT TGATGTCCTG AGTGTAGCAG TCAAGAAACG TGTCTTGTGT 
TTACCTAGGG ATGAAAACCT GACAGCAAAT GAAGTTTTGA AAACGTGTGA TAGGAAAGCA 
AATGTTGCAA TCCTGTTTTC TGGGGGCATT GATTCCATGG TTATTGCAAC CCTTGCTGAC 
CGTCATATTC CTTTAGATGA ACCAATTGAT CTTCTTAATG TAGCTTTCAT AGCTGAAGAA 
AAGACCATGC CAACTACCTT TAACAGAGAA GGGAATAAAC AGAAAAATAA ATGTGAAATA 
CCTTCAGAAG AATTCTCTAA AGATGTTGCT GCTGCTGCTG CTGACAGTCC TAATAAACAT 
GTCAGTGTAC CAGATCGAAT CACAGGAAGG GCGGGACTAA AGGAACTACA AGCTGTTAGC 
CCTTCCCGAA TTTGGAATTT TGTTGAAATT AATGTTTCTA TGGAAGAACT GCAGAAATTA 
AGAAGAACTC GAATATGTCA CTTAATTCGG CCATTGGATA CAGTTTTGGA TGATAGCATT 
GGCTGTGCAG TCTGGTTTGC TTCTAGAGGA ATTGGTTGGT TAGTGGCCCA GGAAGGAGTG 
AAATCCTATC AGAGCAATGC AAAGGTAGTT CTCACTGGAA TTGGTGCAGA TGAGCAACTT 
GCAGGTTATT CTCGTCATCG TGTCCGCTTT CAGTCGCATG GGCTGGAAGG ATTGAATAAG 
GAAATAATGA TGGAACTGGG TCGAATTTCT TCTAGAAATC TTGGTCGTGA TGACAGAGTT 
ATTGGTGATC ATGGAAAAGA AGCAAGATTT CCTTTCCTGG ATGAAAATGT TGTCTCCTTT 
CTAAATTCTC TGCCGATTTG GGAAAAAGCA AACTTGACTT TACCCCGAGG AATTGGTGAA 
AAATTACTTT TACGCCTTGC AGCTGTGGAA CTTGGTCTTA CAGCCTCTGC TCTTCTGCCC 
AAACGGGCCA TGCAGTTTGG ATCAAGAATT GCAAAAATGG AAAAAATTAA TGAAAAGGCA 
TCTGATAAAT GTGGACGGCT CCAAATCATG TCCTTAGAAA ATCTTTCTAT TGAAAAGGAG 
ACTAAATTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001340426.1
CDS	381..2312
Translation	MCGICCSVNFSAEHFSQDLKEDLLYNLKQRGPNSSKQLLKSDVNYQCLFSAHVLHLRGVLTTQPVEDERGNVFLWNGEIFSGIKVEAEENDTQILFNYLSSCKNESEILSLFSEVQGPWSFIYYQASSHYLWFGRDFFGRRSLLWHFSNLGKSFCLSSVGTQTSGLANQWQEVPASGLFRIDLKSTVISGCIILQLYPWKYISRENIIEENVNSLSQISADLPAFVSVVANEAKLYLEKPVVPLNMMLPQAALETHCSNISNVPPTREILQVFLTDVHMKEVIQQFIDVLSVAVKKRVLCLPRDENLTANEVLKTCDRKANVAILFSGGIDSMVIATLADRHIPLDEPIDLLNVAFIAEEKTMPTTFNREGNKQKNKCEIPSEEFSKDVAAAAADSPNKHVSVPDRITGRAGLKELQAVSPSRIWNFVEINVSMEELQKLRRTRICHLIRPLDTVLDDSIGCAVWFASRGIGWLVAQEGVKSYQSNAKVVLTGIGADEQLAGYSRHRVRFQSHGLEGLNKEIMMELGRISSRNLGRDDRVIGDHGKEARFPFLDENVVSFLNSLPIWEKANLTLPRGIGEKLLLRLAAVELGLTASALLPKRAMQFGSRIAKMEKINEKASDKCGRLQIMSLENLSIEKETKL

Target ORF information:

RefSeq Version	NM_001353497.1
Organism	Homo sapiens(human)
Definition	Homo sapiens asparagine synthetase domain containing 1 (ASNSD1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353497.1

ORF Insert Sequence:

ATGTGTGGCA TTTGTTGTTC TGTAAACTTT TCTGCTGAGC ATTTCAGTCA AGATTTAAAA 
GAGGACTTAC TATATAATCT TAAACAGCGG GGACCCAATA GTAGTAAACA ATTGTTAAAG 
TCTGATGTTA ACTACCAGTG TTTATTTTCT GCTCACGTCC TACACTTGAG GGGTGTTTTG 
ACTACCCAGC CTGTGGAAGA TGAAAGAGGC AATGTGTTTC TATGGAATGG AGAAATTTTT 
AGTGGAATAA AGGTTGAAGC TGAAGAGAAT GACACTCAAA TTTTGTTTAA TTATCTTTCC 
TCCTGTAAGA ATGAATCTGA GATTTTGTCA CTCTTCTCAG AAGTACAAGG TCCCTGGTCA 
TTTATATATT ATCAAGCATC TAGTCATTAT TTATGGTTTG GTAGGGATTT TTTTGGTCGC 
CGTAGCTTGC TTTGGCATTT TAGTAATTTG GGCAAGAGTT TCTGCCTCTC TTCAGTTGGC 
ACCCAAACAT CTGGATTGGC AAATCAGTGG CAAGAAGTTC CAGCATCTGG ACTTTTCAGA 
ATTGATCTTA AGTCTACTGT CATTTCCGGA TGCATTATTT TACAACTGTA TCCTTGGAAA 
TATATTTCTA GGGAGAATAT TATTGAAGAA AATGTTAATA GCCTGAGTCA AATTTCAGCA 
GACTTACCAG CATTTGTATC AGTGGTAGCA AATGAAGCCA AACTGTATCT TGAAAAACCT 
GTTGTTCCTT TAAATATGAT GTTGCCACAA GCTGCATTGG AGACTCATTG CAGTAATATT 
TCCAATGTGC CACCTACAAG AGAGATACTT CAAGTCTTTC TTACTGATGT ACACATGAAG 
GAAGTAATTC AGCAGTTCAT TGATGTCCTG AGTGTAGCAG TCAAGAAACG TGTCTTGTGT 
TTACCTAGGG ATGAAAACCT GACAGCAAAT GAAGTTTTGA AAACGTGTGA TAGGAAAGCA 
AATGTTGCAA TCCTGTTTTC TGGGGGCATT GATTCCATGG TTATTGCAAC CCTTGCTGAC 
CGTCATATTC CTTTAGATGA ACCAATTGAT CTTCTTAATG TAGCTTTCAT AGCTGAAGAA 
AAGACCATGC CAACTACCTT TAACAGAGAA GGGAATAAAC AGAAAAATAA ATGTGAAATA 
CCTTCAGAAG AATTCTCTAA AGATGTTGCT GCTGCTGCTG CTGACAGTCC TAATAAACAT 
GTCAGTGTAC CAGATCGAAT CACAGGAAGG GCGGGACTAA AGGAACTACA AGCTGTTAGC 
CCTTCCCGAA TTTGGAATTT TGTTGAAATT AATGTTTCTA TGGAAGAACT GCAGAAATTA 
AGAAGAACTC GAATATGTCA CTTAATTCGG CCATTGGATA CAGTTTTGGA TGATAGCATT 
GGCTGTGCAG TCTGGTTTGC TTCTAGAGGA ATTGGTTGGT TAGTGGCCCA GGAAGGAGTG 
AAATCCTATC AGAGCAATGC AAAGGTAGTT CTCACTGGAA TTGGTGCAGA TGAGCAACTT 
GCAGGTTATT CTCGTCATCG TGTCCGCTTT CAGTCGCATG GGCTGGAAGG ATTGAATAAG 
GAAATAATGA TGGAACTGGG TCGAATTTCT TCTAGAAATC TTGGTCGTGA TGACAGAGTT 
ATTGGTGATC ATGGAAAAGA AGCAAGATTT CCTTTCCTGG ATGAAAATGT TGTCTCCTTT 
CTAAATTCTC TGCCGATTTG GGAAAAAGCA AACTTGACTT TACCCCGAGG AATTGGTGAA 
AAATTACTTT TACGCCTTGC AGCTGTGGAA CTTGGTCTTA CAGCCTCTGC TCTTCTGCCC 
AAACGGGCCA TGCAGTTTGG ATCAAGAATT GCAAAAATGG AAAAAATTAA TGAAAAGGCA 
TCTGATAAAT GTGGACGGCT CCAAATCATG TCCTTAGAAA ATCTTTCTAT TGAAAAGGAG 
ACTAAATTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASNSD1 ( XM_017004381.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859870.1 Homo sapiens asparagine synthetase domain containing 1 (ASNSD1), transcript variant X1, mRNA.

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CloneID	OHu36369
Clone ID Related Accession (Same CDS sequence)	NM_019048.4 , XM_017004381.1 , NM_001353498.1 , NM_019048.3 , XM_017004382.1 , NM_001353497.1
Accession Version	XM_017004381.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	asparagine synthetase domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCA TTTGTTGTTC TGTAAACTTT TCTGCTGAGC ATTTCAGTCA AGATTTAAAA 
GAGGACTTAC TATATAATCT TAAACAGCGG GGACCCAATA GTAGTAAACA ATTGTTAAAG 
TCTGATGTTA ACTACCAGTG TTTATTTTCT GCTCACGTCC TACACTTGAG GGGTGTTTTG 
ACTACCCAGC CTGTGGAAGA TGAAAGAGGC AATGTGTTTC TATGGAATGG AGAAATTTTT 
AGTGGAATAA AGGTTGAAGC TGAAGAGAAT GACACTCAAA TTTTGTTTAA TTATCTTTCC 
TCCTGTAAGA ATGAATCTGA GATTTTGTCA CTCTTCTCAG AAGTACAAGG TCCCTGGTCA 
TTTATATATT ATCAAGCATC TAGTCATTAT TTATGGTTTG GTAGGGATTT TTTTGGTCGC 
CGTAGCTTGC TTTGGCATTT TAGTAATTTG GGCAAGAGTT TCTGCCTCTC TTCAGTTGGC 
ACCCAAACAT CTGGATTGGC AAATCAGTGG CAAGAAGTTC CAGCATCTGG ACTTTTCAGA 
ATTGATCTTA AGTCTACTGT CATTTCCGGA TGCATTATTT TACAACTGTA TCCTTGGAAA 
TATATTTCTA GGGAGAATAT TATTGAAGAA AATGTTAATA GCCTGAGTCA AATTTCAGCA 
GACTTACCAG CATTTGTATC AGTGGTAGCA AATGAAGCCA AACTGTATCT TGAAAAACCT 
GTTGTTCCTT TAAATATGAT GTTGCCACAA GCTGCATTGG AGACTCATTG CAGTAATATT 
TCCAATGTGC CACCTACAAG AGAGATACTT CAAGTCTTTC TTACTGATGT ACACATGAAG 
GAAGTAATTC AGCAGTTCAT TGATGTCCTG AGTGTAGCAG TCAAGAAACG TGTCTTGTGT 
TTACCTAGGG ATGAAAACCT GACAGCAAAT GAAGTTTTGA AAACGTGTGA TAGGAAAGCA 
AATGTTGCAA TCCTGTTTTC TGGGGGCATT GATTCCATGG TTATTGCAAC CCTTGCTGAC 
CGTCATATTC CTTTAGATGA ACCAATTGAT CTTCTTAATG TAGCTTTCAT AGCTGAAGAA 
AAGACCATGC CAACTACCTT TAACAGAGAA GGGAATAAAC AGAAAAATAA ATGTGAAATA 
CCTTCAGAAG AATTCTCTAA AGATGTTGCT GCTGCTGCTG CTGACAGTCC TAATAAACAT 
GTCAGTGTAC CAGATCGAAT CACAGGAAGG GCGGGACTAA AGGAACTACA AGCTGTTAGC 
CCTTCCCGAA TTTGGAATTT TGTTGAAATT AATGTTTCTA TGGAAGAACT GCAGAAATTA 
AGAAGAACTC GAATATGTCA CTTAATTCGG CCATTGGATA CAGTTTTGGA TGATAGCATT 
GGCTGTGCAG TCTGGTTTGC TTCTAGAGGA ATTGGTTGGT TAGTGGCCCA GGAAGGAGTG 
AAATCCTATC AGAGCAATGC AAAGGTAGTT CTCACTGGAA TTGGTGCAGA TGAGCAACTT 
GCAGGTTATT CTCGTCATCG TGTCCGCTTT CAGTCGCATG GGCTGGAAGG ATTGAATAAG 
GAAATAATGA TGGAACTGGG TCGAATTTCT TCTAGAAATC TTGGTCGTGA TGACAGAGTT 
ATTGGTGATC ATGGAAAAGA AGCAAGATTT CCTTTCCTGG ATGAAAATGT TGTCTCCTTT 
CTAAATTCTC TGCCGATTTG GGAAAAAGCA AACTTGACTT TACCCCGAGG AATTGGTGAA 
AAATTACTTT TACGCCTTGC AGCTGTGGAA CTTGGTCTTA CAGCCTCTGC TCTTCTGCCC 
AAACGGGCCA TGCAGTTTGG ATCAAGAATT GCAAAAATGG AAAAAATTAA TGAAAAGGCA 
TCTGATAAAT GTGGACGGCT CCAAATCATG TCCTTAGAAA ATCTTTCTAT TGAAAAGGAG 
ACTAAATTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859870.1
CDS	166..2097
Translation	MCGICCSVNFSAEHFSQDLKEDLLYNLKQRGPNSSKQLLKSDVNYQCLFSAHVLHLRGVLTTQPVEDERGNVFLWNGEIFSGIKVEAEENDTQILFNYLSSCKNESEILSLFSEVQGPWSFIYYQASSHYLWFGRDFFGRRSLLWHFSNLGKSFCLSSVGTQTSGLANQWQEVPASGLFRIDLKSTVISGCIILQLYPWKYISRENIIEENVNSLSQISADLPAFVSVVANEAKLYLEKPVVPLNMMLPQAALETHCSNISNVPPTREILQVFLTDVHMKEVIQQFIDVLSVAVKKRVLCLPRDENLTANEVLKTCDRKANVAILFSGGIDSMVIATLADRHIPLDEPIDLLNVAFIAEEKTMPTTFNREGNKQKNKCEIPSEEFSKDVAAAAADSPNKHVSVPDRITGRAGLKELQAVSPSRIWNFVEINVSMEELQKLRRTRICHLIRPLDTVLDDSIGCAVWFASRGIGWLVAQEGVKSYQSNAKVVLTGIGADEQLAGYSRHRVRFQSHGLEGLNKEIMMELGRISSRNLGRDDRVIGDHGKEARFPFLDENVVSFLNSLPIWEKANLTLPRGIGEKLLLRLAAVELGLTASALLPKRAMQFGSRIAKMEKINEKASDKCGRLQIMSLENLSIEKETKL

Target ORF information:

RefSeq Version	XM_017004381.1
Organism	Homo sapiens(human)
Definition	Homo sapiens asparagine synthetase domain containing 1 (ASNSD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004381.1

ORF Insert Sequence:

ATGTGTGGCA TTTGTTGTTC TGTAAACTTT TCTGCTGAGC ATTTCAGTCA AGATTTAAAA 
GAGGACTTAC TATATAATCT TAAACAGCGG GGACCCAATA GTAGTAAACA ATTGTTAAAG 
TCTGATGTTA ACTACCAGTG TTTATTTTCT GCTCACGTCC TACACTTGAG GGGTGTTTTG 
ACTACCCAGC CTGTGGAAGA TGAAAGAGGC AATGTGTTTC TATGGAATGG AGAAATTTTT 
AGTGGAATAA AGGTTGAAGC TGAAGAGAAT GACACTCAAA TTTTGTTTAA TTATCTTTCC 
TCCTGTAAGA ATGAATCTGA GATTTTGTCA CTCTTCTCAG AAGTACAAGG TCCCTGGTCA 
TTTATATATT ATCAAGCATC TAGTCATTAT TTATGGTTTG GTAGGGATTT TTTTGGTCGC 
CGTAGCTTGC TTTGGCATTT TAGTAATTTG GGCAAGAGTT TCTGCCTCTC TTCAGTTGGC 
ACCCAAACAT CTGGATTGGC AAATCAGTGG CAAGAAGTTC CAGCATCTGG ACTTTTCAGA 
ATTGATCTTA AGTCTACTGT CATTTCCGGA TGCATTATTT TACAACTGTA TCCTTGGAAA 
TATATTTCTA GGGAGAATAT TATTGAAGAA AATGTTAATA GCCTGAGTCA AATTTCAGCA 
GACTTACCAG CATTTGTATC AGTGGTAGCA AATGAAGCCA AACTGTATCT TGAAAAACCT 
GTTGTTCCTT TAAATATGAT GTTGCCACAA GCTGCATTGG AGACTCATTG CAGTAATATT 
TCCAATGTGC CACCTACAAG AGAGATACTT CAAGTCTTTC TTACTGATGT ACACATGAAG 
GAAGTAATTC AGCAGTTCAT TGATGTCCTG AGTGTAGCAG TCAAGAAACG TGTCTTGTGT 
TTACCTAGGG ATGAAAACCT GACAGCAAAT GAAGTTTTGA AAACGTGTGA TAGGAAAGCA 
AATGTTGCAA TCCTGTTTTC TGGGGGCATT GATTCCATGG TTATTGCAAC CCTTGCTGAC 
CGTCATATTC CTTTAGATGA ACCAATTGAT CTTCTTAATG TAGCTTTCAT AGCTGAAGAA 
AAGACCATGC CAACTACCTT TAACAGAGAA GGGAATAAAC AGAAAAATAA ATGTGAAATA 
CCTTCAGAAG AATTCTCTAA AGATGTTGCT GCTGCTGCTG CTGACAGTCC TAATAAACAT 
GTCAGTGTAC CAGATCGAAT CACAGGAAGG GCGGGACTAA AGGAACTACA AGCTGTTAGC 
CCTTCCCGAA TTTGGAATTT TGTTGAAATT AATGTTTCTA TGGAAGAACT GCAGAAATTA 
AGAAGAACTC GAATATGTCA CTTAATTCGG CCATTGGATA CAGTTTTGGA TGATAGCATT 
GGCTGTGCAG TCTGGTTTGC TTCTAGAGGA ATTGGTTGGT TAGTGGCCCA GGAAGGAGTG 
AAATCCTATC AGAGCAATGC AAAGGTAGTT CTCACTGGAA TTGGTGCAGA TGAGCAACTT 
GCAGGTTATT CTCGTCATCG TGTCCGCTTT CAGTCGCATG GGCTGGAAGG ATTGAATAAG 
GAAATAATGA TGGAACTGGG TCGAATTTCT TCTAGAAATC TTGGTCGTGA TGACAGAGTT 
ATTGGTGATC ATGGAAAAGA AGCAAGATTT CCTTTCCTGG ATGAAAATGT TGTCTCCTTT 
CTAAATTCTC TGCCGATTTG GGAAAAAGCA AACTTGACTT TACCCCGAGG AATTGGTGAA 
AAATTACTTT TACGCCTTGC AGCTGTGGAA CTTGGTCTTA CAGCCTCTGC TCTTCTGCCC 
AAACGGGCCA TGCAGTTTGG ATCAAGAATT GCAAAAATGG AAAAAATTAA TGAAAAGGCA 
TCTGATAAAT GTGGACGGCT CCAAATCATG TCCTTAGAAA ATCTTTCTAT TGAAAAGGAG 
ACTAAATTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASNSD1 ( XM_017004382.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859871.1 Homo sapiens asparagine synthetase domain containing 1 (ASNSD1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu36369
Clone ID Related Accession (Same CDS sequence)	NM_019048.4 , XM_017004381.1 , NM_001353498.1 , NM_019048.3 , XM_017004382.1 , NM_001353497.1
Accession Version	XM_017004382.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	asparagine synthetase domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCA TTTGTTGTTC TGTAAACTTT TCTGCTGAGC ATTTCAGTCA AGATTTAAAA 
GAGGACTTAC TATATAATCT TAAACAGCGG GGACCCAATA GTAGTAAACA ATTGTTAAAG 
TCTGATGTTA ACTACCAGTG TTTATTTTCT GCTCACGTCC TACACTTGAG GGGTGTTTTG 
ACTACCCAGC CTGTGGAAGA TGAAAGAGGC AATGTGTTTC TATGGAATGG AGAAATTTTT 
AGTGGAATAA AGGTTGAAGC TGAAGAGAAT GACACTCAAA TTTTGTTTAA TTATCTTTCC 
TCCTGTAAGA ATGAATCTGA GATTTTGTCA CTCTTCTCAG AAGTACAAGG TCCCTGGTCA 
TTTATATATT ATCAAGCATC TAGTCATTAT TTATGGTTTG GTAGGGATTT TTTTGGTCGC 
CGTAGCTTGC TTTGGCATTT TAGTAATTTG GGCAAGAGTT TCTGCCTCTC TTCAGTTGGC 
ACCCAAACAT CTGGATTGGC AAATCAGTGG CAAGAAGTTC CAGCATCTGG ACTTTTCAGA 
ATTGATCTTA AGTCTACTGT CATTTCCGGA TGCATTATTT TACAACTGTA TCCTTGGAAA 
TATATTTCTA GGGAGAATAT TATTGAAGAA AATGTTAATA GCCTGAGTCA AATTTCAGCA 
GACTTACCAG CATTTGTATC AGTGGTAGCA AATGAAGCCA AACTGTATCT TGAAAAACCT 
GTTGTTCCTT TAAATATGAT GTTGCCACAA GCTGCATTGG AGACTCATTG CAGTAATATT 
TCCAATGTGC CACCTACAAG AGAGATACTT CAAGTCTTTC TTACTGATGT ACACATGAAG 
GAAGTAATTC AGCAGTTCAT TGATGTCCTG AGTGTAGCAG TCAAGAAACG TGTCTTGTGT 
TTACCTAGGG ATGAAAACCT GACAGCAAAT GAAGTTTTGA AAACGTGTGA TAGGAAAGCA 
AATGTTGCAA TCCTGTTTTC TGGGGGCATT GATTCCATGG TTATTGCAAC CCTTGCTGAC 
CGTCATATTC CTTTAGATGA ACCAATTGAT CTTCTTAATG TAGCTTTCAT AGCTGAAGAA 
AAGACCATGC CAACTACCTT TAACAGAGAA GGGAATAAAC AGAAAAATAA ATGTGAAATA 
CCTTCAGAAG AATTCTCTAA AGATGTTGCT GCTGCTGCTG CTGACAGTCC TAATAAACAT 
GTCAGTGTAC CAGATCGAAT CACAGGAAGG GCGGGACTAA AGGAACTACA AGCTGTTAGC 
CCTTCCCGAA TTTGGAATTT TGTTGAAATT AATGTTTCTA TGGAAGAACT GCAGAAATTA 
AGAAGAACTC GAATATGTCA CTTAATTCGG CCATTGGATA CAGTTTTGGA TGATAGCATT 
GGCTGTGCAG TCTGGTTTGC TTCTAGAGGA ATTGGTTGGT TAGTGGCCCA GGAAGGAGTG 
AAATCCTATC AGAGCAATGC AAAGGTAGTT CTCACTGGAA TTGGTGCAGA TGAGCAACTT 
GCAGGTTATT CTCGTCATCG TGTCCGCTTT CAGTCGCATG GGCTGGAAGG ATTGAATAAG 
GAAATAATGA TGGAACTGGG TCGAATTTCT TCTAGAAATC TTGGTCGTGA TGACAGAGTT 
ATTGGTGATC ATGGAAAAGA AGCAAGATTT CCTTTCCTGG ATGAAAATGT TGTCTCCTTT 
CTAAATTCTC TGCCGATTTG GGAAAAAGCA AACTTGACTT TACCCCGAGG AATTGGTGAA 
AAATTACTTT TACGCCTTGC AGCTGTGGAA CTTGGTCTTA CAGCCTCTGC TCTTCTGCCC 
AAACGGGCCA TGCAGTTTGG ATCAAGAATT GCAAAAATGG AAAAAATTAA TGAAAAGGCA 
TCTGATAAAT GTGGACGGCT CCAAATCATG TCCTTAGAAA ATCTTTCTAT TGAAAAGGAG 
ACTAAATTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859871.1
CDS	951..2882
Translation	MCGICCSVNFSAEHFSQDLKEDLLYNLKQRGPNSSKQLLKSDVNYQCLFSAHVLHLRGVLTTQPVEDERGNVFLWNGEIFSGIKVEAEENDTQILFNYLSSCKNESEILSLFSEVQGPWSFIYYQASSHYLWFGRDFFGRRSLLWHFSNLGKSFCLSSVGTQTSGLANQWQEVPASGLFRIDLKSTVISGCIILQLYPWKYISRENIIEENVNSLSQISADLPAFVSVVANEAKLYLEKPVVPLNMMLPQAALETHCSNISNVPPTREILQVFLTDVHMKEVIQQFIDVLSVAVKKRVLCLPRDENLTANEVLKTCDRKANVAILFSGGIDSMVIATLADRHIPLDEPIDLLNVAFIAEEKTMPTTFNREGNKQKNKCEIPSEEFSKDVAAAAADSPNKHVSVPDRITGRAGLKELQAVSPSRIWNFVEINVSMEELQKLRRTRICHLIRPLDTVLDDSIGCAVWFASRGIGWLVAQEGVKSYQSNAKVVLTGIGADEQLAGYSRHRVRFQSHGLEGLNKEIMMELGRISSRNLGRDDRVIGDHGKEARFPFLDENVVSFLNSLPIWEKANLTLPRGIGEKLLLRLAAVELGLTASALLPKRAMQFGSRIAKMEKINEKASDKCGRLQIMSLENLSIEKETKL

Target ORF information:

RefSeq Version	XM_017004382.1
Organism	Homo sapiens(human)
Definition	Homo sapiens asparagine synthetase domain containing 1 (ASNSD1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004382.1

ORF Insert Sequence:

ATGTGTGGCA TTTGTTGTTC TGTAAACTTT TCTGCTGAGC ATTTCAGTCA AGATTTAAAA 
GAGGACTTAC TATATAATCT TAAACAGCGG GGACCCAATA GTAGTAAACA ATTGTTAAAG 
TCTGATGTTA ACTACCAGTG TTTATTTTCT GCTCACGTCC TACACTTGAG GGGTGTTTTG 
ACTACCCAGC CTGTGGAAGA TGAAAGAGGC AATGTGTTTC TATGGAATGG AGAAATTTTT 
AGTGGAATAA AGGTTGAAGC TGAAGAGAAT GACACTCAAA TTTTGTTTAA TTATCTTTCC 
TCCTGTAAGA ATGAATCTGA GATTTTGTCA CTCTTCTCAG AAGTACAAGG TCCCTGGTCA 
TTTATATATT ATCAAGCATC TAGTCATTAT TTATGGTTTG GTAGGGATTT TTTTGGTCGC 
CGTAGCTTGC TTTGGCATTT TAGTAATTTG GGCAAGAGTT TCTGCCTCTC TTCAGTTGGC 
ACCCAAACAT CTGGATTGGC AAATCAGTGG CAAGAAGTTC CAGCATCTGG ACTTTTCAGA 
ATTGATCTTA AGTCTACTGT CATTTCCGGA TGCATTATTT TACAACTGTA TCCTTGGAAA 
TATATTTCTA GGGAGAATAT TATTGAAGAA AATGTTAATA GCCTGAGTCA AATTTCAGCA 
GACTTACCAG CATTTGTATC AGTGGTAGCA AATGAAGCCA AACTGTATCT TGAAAAACCT 
GTTGTTCCTT TAAATATGAT GTTGCCACAA GCTGCATTGG AGACTCATTG CAGTAATATT 
TCCAATGTGC CACCTACAAG AGAGATACTT CAAGTCTTTC TTACTGATGT ACACATGAAG 
GAAGTAATTC AGCAGTTCAT TGATGTCCTG AGTGTAGCAG TCAAGAAACG TGTCTTGTGT 
TTACCTAGGG ATGAAAACCT GACAGCAAAT GAAGTTTTGA AAACGTGTGA TAGGAAAGCA 
AATGTTGCAA TCCTGTTTTC TGGGGGCATT GATTCCATGG TTATTGCAAC CCTTGCTGAC 
CGTCATATTC CTTTAGATGA ACCAATTGAT CTTCTTAATG TAGCTTTCAT AGCTGAAGAA 
AAGACCATGC CAACTACCTT TAACAGAGAA GGGAATAAAC AGAAAAATAA ATGTGAAATA 
CCTTCAGAAG AATTCTCTAA AGATGTTGCT GCTGCTGCTG CTGACAGTCC TAATAAACAT 
GTCAGTGTAC CAGATCGAAT CACAGGAAGG GCGGGACTAA AGGAACTACA AGCTGTTAGC 
CCTTCCCGAA TTTGGAATTT TGTTGAAATT AATGTTTCTA TGGAAGAACT GCAGAAATTA 
AGAAGAACTC GAATATGTCA CTTAATTCGG CCATTGGATA CAGTTTTGGA TGATAGCATT 
GGCTGTGCAG TCTGGTTTGC TTCTAGAGGA ATTGGTTGGT TAGTGGCCCA GGAAGGAGTG 
AAATCCTATC AGAGCAATGC AAAGGTAGTT CTCACTGGAA TTGGTGCAGA TGAGCAACTT 
GCAGGTTATT CTCGTCATCG TGTCCGCTTT CAGTCGCATG GGCTGGAAGG ATTGAATAAG 
GAAATAATGA TGGAACTGGG TCGAATTTCT TCTAGAAATC TTGGTCGTGA TGACAGAGTT 
ATTGGTGATC ATGGAAAAGA AGCAAGATTT CCTTTCCTGG ATGAAAATGT TGTCTCCTTT 
CTAAATTCTC TGCCGATTTG GGAAAAAGCA AACTTGACTT TACCCCGAGG AATTGGTGAA 
AAATTACTTT TACGCCTTGC AGCTGTGGAA CTTGGTCTTA CAGCCTCTGC TCTTCTGCCC 
AAACGGGCCA TGCAGTTTGG ATCAAGAATT GCAAAAATGG AAAAAATTAA TGAAAAGGCA 
TCTGATAAAT GTGGACGGCT CCAAATCATG TCCTTAGAAA ATCTTTCTAT TGAAAAGGAG 
ACTAAATTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Construction of eukaryotic expression plasmid containing HCV NS3 segment and protein expression in human HL-7702 hepatocytes.
World journal of gastroenterology12(7)1038-42(2006 Feb)
Li JW,Li K,Jiang J,Xu XL,Huang ZQ

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ASNSD1 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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