USP53 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following USP53 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the USP53 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu38976 XM_011532038.2
Latest version!
Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu38976 XM_006714240.1
Latest version!
Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu38977 XM_005263077.4
Latest version!
Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu38977 XM_017008317.2
Latest version!
Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu38977 XM_017008318.2
Latest version!
Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu38977 NM_001371398.1
Latest version!
Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu38977 NM_001371397.1
Latest version!
Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu02420 NM_001371395.1
Latest version!
Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu02420 NM_001371399.1
Latest version!
Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu38975 NM_001371396.1
Latest version!
Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu02420 NM_019050.2
Latest version!
Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu02420 XM_017008315.2
Latest version!
Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu02420 XM_017008314.1
Latest version!
Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu02420 XM_017008313.1
Latest version!
Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu02420 XM_011532037.2
Latest version!
Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu02420 XM_017008312.2
Latest version!
Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu02420 XM_005263073.4
Latest version!
Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu38974 XM_005263075.3
Latest version!
Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu38975 XM_005263076.3
Latest version!
Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu38975 XM_017008316.2
Latest version!
Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
View Old Accession Versions >>
OHu38977 XM_017008318.1 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu02420 XM_005263073.3 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu38975 XM_017008316.1 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu02420 XM_017008312.1 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu38975 XM_005263076.2 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu38977 XM_005263077.3 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu38977 XM_017008317.1 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu38974 XM_005263075.2 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu02420 XM_017008315.1 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu38976
    Clone ID Related Accession (Same CDS sequence) XM_006714240.1 , XM_011532038.2
    Accession Version XM_006714240.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2874bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGATGATG CTGCGGAGTG CTTTGAAAAT ATGTTGGAGA GGATTCATTT TCACATAGTG 
    CCAAGCAGAG ATGCAGACAT GTGTACCTCT AAATCTTGTA TCACTCACCA GAAGTTTGCT
    ATGACTCTGT ATGAACAGTG TGTGTGTCGT AGCTGTGGAG CATCGTCAGA TCCTCTACCT
    TTTACAGAAT TTGTGCGGTA CATTTCTACA ACAGCCTTAT GCAATGAGGT TGAAAGAATG
    TTGGAAAGGC ATGAACGCTT TAAACCTGAA ATGTTTGCAG AATTGCTACA AGCAGCAAAT
    ACAACAGATG ACTATAGGAA ATGTCCTAGT AACTGTGGCC AAAAAATAAA AATTCGCCGT
    GTTTTAATGA ATTGCCCAGA GATTGTTACA ATTGGTTTAG TCTGGGACTC CGAGCATTCT
    GACTTGACCG AAGCTGTTGT TCGGAATCTA GCAACACATC TTTATCTTCC TGGGCTTTTT
    TATAGAGTTA CTGATGAAAA TGCCAAAAAT AGTGAACTTA ACCTTGTTGG TATGATCTGC
    TACACCAGCC AACATTATTG TGCCTTTGCA TTTCACACCA AAAGTTCCAA ATGGGTATTT
    TTTGATGATG CAAATGTGAA AGAGATTGGA ACTAGATGGA AAGATGTTGT CTCCAAATGC
    ATTCGATGCC ACTTTCAGCC ACTACTTTTG TTTTATGCAA ACCCAGATGG CACAGCAGTT
    TCTACTGAGG ATGCACTCAG GCAGGTCATC AGCTGGTCAC ATTACAAATC TGTTGCAGAA
    AATATGGGAT GTGAAAAGCC TGTAATTCAT AAGTCAGATA ATTTAAAAGA AAATGGATTT
    GGTGATCAGG CAAAGCAGAG AGAAAATCAG AAATTTCCAA CTGATAATAT TTCATCATCT
    AATCGGAGCC ACAGTCACAC AGGTGTAGGG AAAGGACCAG CTAAGTTAAG TCACATTGAT
    CAAAGGGAAA AGATAAAAGA CATTTCCAGA GAATGTGCTC TGAAAGCTAT TGAACAGAAA
    AACTTACTTT CTTCACAAAG GAAAGATTTA GAGAAGGGAC AAAGAAAAGA TTTAGGACGA
    CATAGAGATT TGGTTGATGA AGACCTTTCA CATTTCCAAT CTGGATCACC TCCTGCCCCA
    AATGGTTTTA AACAACATGG GAATCCACAT CTATATCATA GTCAAGGAAA AGGATCATAT
    AAACATGACC GAGTTGTACC TCAGAGTCGA GCTTCTGCAC AAATAATAAG TTCAAGTAAA
    TCCCAGATTC TTGCTCCAGG AGAGAAAATA ACTGGCAAAG TTAAGAGTGA CAATGGCACT
    GGATATGACA CAGACAGCAG CCAAGATTCT AGGGATAGAG GAAACAGCTG TGATAGCAGC
    AGTAAAAGCC GGAACCGAGG TTGGAAACCT ATGAGAGAAA CATTAAATGT TGATAGTATT
    TTTAGTGAAA GTGAAAAAAG ACAGCATAGT CCAAGACATA AACCAAATAT CAGTAATAAG
    CCTAAATCTA GCAAGGATCC GAGTTTTAGT AATTGGCCAA AAGAGAATCC AAAGCAAAAA
    GGTTTAATGA CCATATATGA AGATGAAATG AAGCAGGAAA TAGGAAGCAG AAGTTCCCTT
    GAATCTAATG GAAAAGGAGC AGAGAAAAAT AAAGGCCTTG TAGAGGGTAA AGTGCATGGT
    GATAATTGGC AGATGCAAAG GACTGAGTCT GGATATGAAA GCAGTGATCA CATCAGTAAT
    GGTTCTACTA ATTTGGACTC ACCTGTTATC GATGGAAATG GTACAGTAAT GGATATCAGT
    GGTGTTAAAG AAACAGTATG CTTCAGTGAC CAGATTACGA CAAGCAACCT AAATAAAGAA
    CGTGGGGACT GTACCTCCCT TCAGAGCCAA CATCACTTAG AAGGCTTTAG AAAAGAACTC
    AGGAATTTGG AAGCAGGCTA TAAATCTCAT GAATTCCACC CAGAATCACA TTTACAAATA
    AAAAATCATT TGATAAAAAG ATCACATGTA CATGAAGACA ATGGAAAGTT ATTTCCTTCA
    TCCAGTCTAC AAATACCCAA GGACCATAAT GCAAGAGAAC ATATCCACCA GTCAGATGAA
    CAGAAACTTG AAAAACCGAA TGAATGCAAA TTTTCTGAGT GGCTTAATAT AGAAAATTCT
    GAGAGAACAG GTTTGCCTTT TCACGTTGAT AACTCTGCTT CTGGGAAGAG AGTGAACAGT
    AATGAACCAT CTTCATTATG GTCTTCACAC CTAAGAACTG TTGGGTTAAA GCCAGAAACT
    GCTCCTCTCA TCCAGCAACA AAATATCATG GATCAATGTT ACTTTGAGAA CTCTCTATCC
    ACAGAATGTA TAATTCGGTC AGCCAGCAGA TCTGATGGGT GTCAGATGCC AAAACTTTTT
    TGCCAGAATC TACCACCCCC TTTGCCACCA AAGAAATATG CTATAACCAG TGTGCCACAG
    TCAGAGAAAA GCGAATCTAC ACCTGATGTC AAACTTACAG AGGTGTTTAA AGCTACCTCT
    CATCTTCCGA AGCACAGTTT AAGTACAGCT TCAGAACCAA GTTTAGAAGT GAGTACACAT
    ATGAATGATG AAAGACATAA AGAAACATTT CAAGTGAGAG AATGTTTTGG CAACACACCA
    AACTGTCCAT CCAGCTCCTC AACTAACGAT TTTCAGGCAA ACTCAGGTGC CATTGATGCA
    TTTTGCCAAC CAGAACTAGA CTCTATTTCT ACCTGTCCAA ATGAGACAGT TTCATTAACT
    ACCTATTTTT CAGTTGATAG CTGCATGACG GATACATATA GATTGAAATA CCATCAGAGG
    CCCAAGCTCT CTTTTCCAGA GAGCAGTGGC TTTTGTAATA ATTCACTATC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006714303.1
    CDS490..3363
    Translation

    Target ORF information:

    RefSeq Version XM_006714240.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006714240.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGATGATG CTGCGGAGTG CTTTGAAAAT ATGTTGGAGA GGATTCATTT TCACATAGTG 
    CCAAGCAGAG ATGCAGACAT GTGTACCTCT AAATCTTGTA TCACTCACCA GAAGTTTGCT
    ATGACTCTGT ATGAACAGTG TGTGTGTCGT AGCTGTGGAG CATCGTCAGA TCCTCTACCT
    TTTACAGAAT TTGTGCGGTA CATTTCTACA ACAGCCTTAT GCAATGAGGT TGAAAGAATG
    TTGGAAAGGC ATGAACGCTT TAAACCTGAA ATGTTTGCAG AATTGCTACA AGCAGCAAAT
    ACAACAGATG ACTATAGGAA ATGTCCTAGT AACTGTGGCC AAAAAATAAA AATTCGCCGT
    GTTTTAATGA ATTGCCCAGA GATTGTTACA ATTGGTTTAG TCTGGGACTC CGAGCATTCT
    GACTTGACCG AAGCTGTTGT TCGGAATCTA GCAACACATC TTTATCTTCC TGGGCTTTTT
    TATAGAGTTA CTGATGAAAA TGCCAAAAAT AGTGAACTTA ACCTTGTTGG TATGATCTGC
    TACACCAGCC AACATTATTG TGCCTTTGCA TTTCACACCA AAAGTTCCAA ATGGGTATTT
    TTTGATGATG CAAATGTGAA AGAGATTGGA ACTAGATGGA AAGATGTTGT CTCCAAATGC
    ATTCGATGCC ACTTTCAGCC ACTACTTTTG TTTTATGCAA ACCCAGATGG CACAGCAGTT
    TCTACTGAGG ATGCACTCAG GCAGGTCATC AGCTGGTCAC ATTACAAATC TGTTGCAGAA
    AATATGGGAT GTGAAAAGCC TGTAATTCAT AAGTCAGATA ATTTAAAAGA AAATGGATTT
    GGTGATCAGG CAAAGCAGAG AGAAAATCAG AAATTTCCAA CTGATAATAT TTCATCATCT
    AATCGGAGCC ACAGTCACAC AGGTGTAGGG AAAGGACCAG CTAAGTTAAG TCACATTGAT
    CAAAGGGAAA AGATAAAAGA CATTTCCAGA GAATGTGCTC TGAAAGCTAT TGAACAGAAA
    AACTTACTTT CTTCACAAAG GAAAGATTTA GAGAAGGGAC AAAGAAAAGA TTTAGGACGA
    CATAGAGATT TGGTTGATGA AGACCTTTCA CATTTCCAAT CTGGATCACC TCCTGCCCCA
    AATGGTTTTA AACAACATGG GAATCCACAT CTATATCATA GTCAAGGAAA AGGATCATAT
    AAACATGACC GAGTTGTACC TCAGAGTCGA GCTTCTGCAC AAATAATAAG TTCAAGTAAA
    TCCCAGATTC TTGCTCCAGG AGAGAAAATA ACTGGCAAAG TTAAGAGTGA CAATGGCACT
    GGATATGACA CAGACAGCAG CCAAGATTCT AGGGATAGAG GAAACAGCTG TGATAGCAGC
    AGTAAAAGCC GGAACCGAGG TTGGAAACCT ATGAGAGAAA CATTAAATGT TGATAGTATT
    TTTAGTGAAA GTGAAAAAAG ACAGCATAGT CCAAGACATA AACCAAATAT CAGTAATAAG
    CCTAAATCTA GCAAGGATCC GAGTTTTAGT AATTGGCCAA AAGAGAATCC AAAGCAAAAA
    GGTTTAATGA CCATATATGA AGATGAAATG AAGCAGGAAA TAGGAAGCAG AAGTTCCCTT
    GAATCTAATG GAAAAGGAGC AGAGAAAAAT AAAGGCCTTG TAGAGGGTAA AGTGCATGGT
    GATAATTGGC AGATGCAAAG GACTGAGTCT GGATATGAAA GCAGTGATCA CATCAGTAAT
    GGTTCTACTA ATTTGGACTC ACCTGTTATC GATGGAAATG GTACAGTAAT GGATATCAGT
    GGTGTTAAAG AAACAGTATG CTTCAGTGAC CAGATTACGA CAAGCAACCT AAATAAAGAA
    CGTGGGGACT GTACCTCCCT TCAGAGCCAA CATCACTTAG AAGGCTTTAG AAAAGAACTC
    AGGAATTTGG AAGCAGGCTA TAAATCTCAT GAATTCCACC CAGAATCACA TTTACAAATA
    AAAAATCATT TGATAAAAAG ATCACATGTA CATGAAGACA ATGGAAAGTT ATTTCCTTCA
    TCCAGTCTAC AAATACCCAA GGACCATAAT GCAAGAGAAC ATATCCACCA GTCAGATGAA
    CAGAAACTTG AAAAACCGAA TGAATGCAAA TTTTCTGAGT GGCTTAATAT AGAAAATTCT
    GAGAGAACAG GTTTGCCTTT TCACGTTGAT AACTCTGCTT CTGGGAAGAG AGTGAACAGT
    AATGAACCAT CTTCATTATG GTCTTCACAC CTAAGAACTG TTGGGTTAAA GCCAGAAACT
    GCTCCTCTCA TCCAGCAACA AAATATCATG GATCAATGTT ACTTTGAGAA CTCTCTATCC
    ACAGAATGTA TAATTCGGTC AGCCAGCAGA TCTGATGGGT GTCAGATGCC AAAACTTTTT
    TGCCAGAATC TACCACCCCC TTTGCCACCA AAGAAATATG CTATAACCAG TGTGCCACAG
    TCAGAGAAAA GCGAATCTAC ACCTGATGTC AAACTTACAG AGGTGTTTAA AGCTACCTCT
    CATCTTCCGA AGCACAGTTT AAGTACAGCT TCAGAACCAA GTTTAGAAGT GAGTACACAT
    ATGAATGATG AAAGACATAA AGAAACATTT CAAGTGAGAG AATGTTTTGG CAACACACCA
    AACTGTCCAT CCAGCTCCTC AACTAACGAT TTTCAGGCAA ACTCAGGTGC CATTGATGCA
    TTTTGCCAAC CAGAACTAGA CTCTATTTCT ACCTGTCCAA ATGAGACAGT TTCATTAACT
    ACCTATTTTT CAGTTGATAG CTGCATGACG GATACATATA GATTGAAATA CCATCAGAGG
    CCCAAGCTCT CTTTTCCAGA GAGCAGTGGC TTTTGTAATA ATTCACTATC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38976
    Clone ID Related Accession (Same CDS sequence) XM_006714240.1 , XM_011532038.2
    Accession Version XM_011532038.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2874bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011532038.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGATGATG CTGCGGAGTG CTTTGAAAAT ATGTTGGAGA GGATTCATTT TCACATAGTG 
    CCAAGCAGAG ATGCAGACAT GTGTACCTCT AAATCTTGTA TCACTCACCA GAAGTTTGCT
    ATGACTCTGT ATGAACAGTG TGTGTGTCGT AGCTGTGGAG CATCGTCAGA TCCTCTACCT
    TTTACAGAAT TTGTGCGGTA CATTTCTACA ACAGCCTTAT GCAATGAGGT TGAAAGAATG
    TTGGAAAGGC ATGAACGCTT TAAACCTGAA ATGTTTGCAG AATTGCTACA AGCAGCAAAT
    ACAACAGATG ACTATAGGAA ATGTCCTAGT AACTGTGGCC AAAAAATAAA AATTCGCCGT
    GTTTTAATGA ATTGCCCAGA GATTGTTACA ATTGGTTTAG TCTGGGACTC CGAGCATTCT
    GACTTGACCG AAGCTGTTGT TCGGAATCTA GCAACACATC TTTATCTTCC TGGGCTTTTT
    TATAGAGTTA CTGATGAAAA TGCCAAAAAT AGTGAACTTA ACCTTGTTGG TATGATCTGC
    TACACCAGCC AACATTATTG TGCCTTTGCA TTTCACACCA AAAGTTCCAA ATGGGTATTT
    TTTGATGATG CAAATGTGAA AGAGATTGGA ACTAGATGGA AAGATGTTGT CTCCAAATGC
    ATTCGATGCC ACTTTCAGCC ACTACTTTTG TTTTATGCAA ACCCAGATGG CACAGCAGTT
    TCTACTGAGG ATGCACTCAG GCAGGTCATC AGCTGGTCAC ATTACAAATC TGTTGCAGAA
    AATATGGGAT GTGAAAAGCC TGTAATTCAT AAGTCAGATA ATTTAAAAGA AAATGGATTT
    GGTGATCAGG CAAAGCAGAG AGAAAATCAG AAATTTCCAA CTGATAATAT TTCATCATCT
    AATCGGAGCC ACAGTCACAC AGGTGTAGGG AAAGGACCAG CTAAGTTAAG TCACATTGAT
    CAAAGGGAAA AGATAAAAGA CATTTCCAGA GAATGTGCTC TGAAAGCTAT TGAACAGAAA
    AACTTACTTT CTTCACAAAG GAAAGATTTA GAGAAGGGAC AAAGAAAAGA TTTAGGACGA
    CATAGAGATT TGGTTGATGA AGACCTTTCA CATTTCCAAT CTGGATCACC TCCTGCCCCA
    AATGGTTTTA AACAACATGG GAATCCACAT CTATATCATA GTCAAGGAAA AGGATCATAT
    AAACATGACC GAGTTGTACC TCAGAGTCGA GCTTCTGCAC AAATAATAAG TTCAAGTAAA
    TCCCAGATTC TTGCTCCAGG AGAGAAAATA ACTGGCAAAG TTAAGAGTGA CAATGGCACT
    GGATATGACA CAGACAGCAG CCAAGATTCT AGGGATAGAG GAAACAGCTG TGATAGCAGC
    AGTAAAAGCC GGAACCGAGG TTGGAAACCT ATGAGAGAAA CATTAAATGT TGATAGTATT
    TTTAGTGAAA GTGAAAAAAG ACAGCATAGT CCAAGACATA AACCAAATAT CAGTAATAAG
    CCTAAATCTA GCAAGGATCC GAGTTTTAGT AATTGGCCAA AAGAGAATCC AAAGCAAAAA
    GGTTTAATGA CCATATATGA AGATGAAATG AAGCAGGAAA TAGGAAGCAG AAGTTCCCTT
    GAATCTAATG GAAAAGGAGC AGAGAAAAAT AAAGGCCTTG TAGAGGGTAA AGTGCATGGT
    GATAATTGGC AGATGCAAAG GACTGAGTCT GGATATGAAA GCAGTGATCA CATCAGTAAT
    GGTTCTACTA ATTTGGACTC ACCTGTTATC GATGGAAATG GTACAGTAAT GGATATCAGT
    GGTGTTAAAG AAACAGTATG CTTCAGTGAC CAGATTACGA CAAGCAACCT AAATAAAGAA
    CGTGGGGACT GTACCTCCCT TCAGAGCCAA CATCACTTAG AAGGCTTTAG AAAAGAACTC
    AGGAATTTGG AAGCAGGCTA TAAATCTCAT GAATTCCACC CAGAATCACA TTTACAAATA
    AAAAATCATT TGATAAAAAG ATCACATGTA CATGAAGACA ATGGAAAGTT ATTTCCTTCA
    TCCAGTCTAC AAATACCCAA GGACCATAAT GCAAGAGAAC ATATCCACCA GTCAGATGAA
    CAGAAACTTG AAAAACCGAA TGAATGCAAA TTTTCTGAGT GGCTTAATAT AGAAAATTCT
    GAGAGAACAG GTTTGCCTTT TCACGTTGAT AACTCTGCTT CTGGGAAGAG AGTGAACAGT
    AATGAACCAT CTTCATTATG GTCTTCACAC CTAAGAACTG TTGGGTTAAA GCCAGAAACT
    GCTCCTCTCA TCCAGCAACA AAATATCATG GATCAATGTT ACTTTGAGAA CTCTCTATCC
    ACAGAATGTA TAATTCGGTC AGCCAGCAGA TCTGATGGGT GTCAGATGCC AAAACTTTTT
    TGCCAGAATC TACCACCCCC TTTGCCACCA AAGAAATATG CTATAACCAG TGTGCCACAG
    TCAGAGAAAA GCGAATCTAC ACCTGATGTC AAACTTACAG AGGTGTTTAA AGCTACCTCT
    CATCTTCCGA AGCACAGTTT AAGTACAGCT TCAGAACCAA GTTTAGAAGT GAGTACACAT
    ATGAATGATG AAAGACATAA AGAAACATTT CAAGTGAGAG AATGTTTTGG CAACACACCA
    AACTGTCCAT CCAGCTCCTC AACTAACGAT TTTCAGGCAA ACTCAGGTGC CATTGATGCA
    TTTTGCCAAC CAGAACTAGA CTCTATTTCT ACCTGTCCAA ATGAGACAGT TTCATTAACT
    ACCTATTTTT CAGTTGATAG CTGCATGACG GATACATATA GATTGAAATA CCATCAGAGG
    CCCAAGCTCT CTTTTCCAGA GAGCAGTGGC TTTTGTAATA ATTCACTATC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011530340.1
    CDS744..3617
    Translation

    Target ORF information:

    RefSeq Version XM_011532038.2
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011532038.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGATGATG CTGCGGAGTG CTTTGAAAAT ATGTTGGAGA GGATTCATTT TCACATAGTG 
    CCAAGCAGAG ATGCAGACAT GTGTACCTCT AAATCTTGTA TCACTCACCA GAAGTTTGCT
    ATGACTCTGT ATGAACAGTG TGTGTGTCGT AGCTGTGGAG CATCGTCAGA TCCTCTACCT
    TTTACAGAAT TTGTGCGGTA CATTTCTACA ACAGCCTTAT GCAATGAGGT TGAAAGAATG
    TTGGAAAGGC ATGAACGCTT TAAACCTGAA ATGTTTGCAG AATTGCTACA AGCAGCAAAT
    ACAACAGATG ACTATAGGAA ATGTCCTAGT AACTGTGGCC AAAAAATAAA AATTCGCCGT
    GTTTTAATGA ATTGCCCAGA GATTGTTACA ATTGGTTTAG TCTGGGACTC CGAGCATTCT
    GACTTGACCG AAGCTGTTGT TCGGAATCTA GCAACACATC TTTATCTTCC TGGGCTTTTT
    TATAGAGTTA CTGATGAAAA TGCCAAAAAT AGTGAACTTA ACCTTGTTGG TATGATCTGC
    TACACCAGCC AACATTATTG TGCCTTTGCA TTTCACACCA AAAGTTCCAA ATGGGTATTT
    TTTGATGATG CAAATGTGAA AGAGATTGGA ACTAGATGGA AAGATGTTGT CTCCAAATGC
    ATTCGATGCC ACTTTCAGCC ACTACTTTTG TTTTATGCAA ACCCAGATGG CACAGCAGTT
    TCTACTGAGG ATGCACTCAG GCAGGTCATC AGCTGGTCAC ATTACAAATC TGTTGCAGAA
    AATATGGGAT GTGAAAAGCC TGTAATTCAT AAGTCAGATA ATTTAAAAGA AAATGGATTT
    GGTGATCAGG CAAAGCAGAG AGAAAATCAG AAATTTCCAA CTGATAATAT TTCATCATCT
    AATCGGAGCC ACAGTCACAC AGGTGTAGGG AAAGGACCAG CTAAGTTAAG TCACATTGAT
    CAAAGGGAAA AGATAAAAGA CATTTCCAGA GAATGTGCTC TGAAAGCTAT TGAACAGAAA
    AACTTACTTT CTTCACAAAG GAAAGATTTA GAGAAGGGAC AAAGAAAAGA TTTAGGACGA
    CATAGAGATT TGGTTGATGA AGACCTTTCA CATTTCCAAT CTGGATCACC TCCTGCCCCA
    AATGGTTTTA AACAACATGG GAATCCACAT CTATATCATA GTCAAGGAAA AGGATCATAT
    AAACATGACC GAGTTGTACC TCAGAGTCGA GCTTCTGCAC AAATAATAAG TTCAAGTAAA
    TCCCAGATTC TTGCTCCAGG AGAGAAAATA ACTGGCAAAG TTAAGAGTGA CAATGGCACT
    GGATATGACA CAGACAGCAG CCAAGATTCT AGGGATAGAG GAAACAGCTG TGATAGCAGC
    AGTAAAAGCC GGAACCGAGG TTGGAAACCT ATGAGAGAAA CATTAAATGT TGATAGTATT
    TTTAGTGAAA GTGAAAAAAG ACAGCATAGT CCAAGACATA AACCAAATAT CAGTAATAAG
    CCTAAATCTA GCAAGGATCC GAGTTTTAGT AATTGGCCAA AAGAGAATCC AAAGCAAAAA
    GGTTTAATGA CCATATATGA AGATGAAATG AAGCAGGAAA TAGGAAGCAG AAGTTCCCTT
    GAATCTAATG GAAAAGGAGC AGAGAAAAAT AAAGGCCTTG TAGAGGGTAA AGTGCATGGT
    GATAATTGGC AGATGCAAAG GACTGAGTCT GGATATGAAA GCAGTGATCA CATCAGTAAT
    GGTTCTACTA ATTTGGACTC ACCTGTTATC GATGGAAATG GTACAGTAAT GGATATCAGT
    GGTGTTAAAG AAACAGTATG CTTCAGTGAC CAGATTACGA CAAGCAACCT AAATAAAGAA
    CGTGGGGACT GTACCTCCCT TCAGAGCCAA CATCACTTAG AAGGCTTTAG AAAAGAACTC
    AGGAATTTGG AAGCAGGCTA TAAATCTCAT GAATTCCACC CAGAATCACA TTTACAAATA
    AAAAATCATT TGATAAAAAG ATCACATGTA CATGAAGACA ATGGAAAGTT ATTTCCTTCA
    TCCAGTCTAC AAATACCCAA GGACCATAAT GCAAGAGAAC ATATCCACCA GTCAGATGAA
    CAGAAACTTG AAAAACCGAA TGAATGCAAA TTTTCTGAGT GGCTTAATAT AGAAAATTCT
    GAGAGAACAG GTTTGCCTTT TCACGTTGAT AACTCTGCTT CTGGGAAGAG AGTGAACAGT
    AATGAACCAT CTTCATTATG GTCTTCACAC CTAAGAACTG TTGGGTTAAA GCCAGAAACT
    GCTCCTCTCA TCCAGCAACA AAATATCATG GATCAATGTT ACTTTGAGAA CTCTCTATCC
    ACAGAATGTA TAATTCGGTC AGCCAGCAGA TCTGATGGGT GTCAGATGCC AAAACTTTTT
    TGCCAGAATC TACCACCCCC TTTGCCACCA AAGAAATATG CTATAACCAG TGTGCCACAG
    TCAGAGAAAA GCGAATCTAC ACCTGATGTC AAACTTACAG AGGTGTTTAA AGCTACCTCT
    CATCTTCCGA AGCACAGTTT AAGTACAGCT TCAGAACCAA GTTTAGAAGT GAGTACACAT
    ATGAATGATG AAAGACATAA AGAAACATTT CAAGTGAGAG AATGTTTTGG CAACACACCA
    AACTGTCCAT CCAGCTCCTC AACTAACGAT TTTCAGGCAA ACTCAGGTGC CATTGATGCA
    TTTTGCCAAC CAGAACTAGA CTCTATTTCT ACCTGTCCAA ATGAGACAGT TTCATTAACT
    ACCTATTTTT CAGTTGATAG CTGCATGACG GATACATATA GATTGAAATA CCATCAGAGG
    CCCAAGCTCT CTTTTCCAGA GAGCAGTGGC TTTTGTAATA ATTCACTATC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38977
    Clone ID Related Accession (Same CDS sequence) XM_017008318.2 , XM_005263077.3 , XM_017008318.1 , NM_001371398.1 , XM_017008317.1 , XM_005263077.4 , XM_017008317.2 , NM_001371397.1
    Accession Version XM_017008318.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2403bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-12
    Organism Homo sapiens(human)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017008318.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GATCACATGT ACATGAAGAC AATGGAAAGT
    TATTTCCTTC ATCCAGTCTA CAAATACCCA AGGACCATAA TGCAAGAGAA CATATCCACC
    AGTCAGATGA ACAGAAACTT GAAAAACCGA ATGAATGCAA ATTTTCTGAG TGGCTTAATA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863807.1
    CDS1051..3453
    Translation

    Target ORF information:

    RefSeq Version XM_017008318.2
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008318.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GATCACATGT ACATGAAGAC AATGGAAAGT
    TATTTCCTTC ATCCAGTCTA CAAATACCCA AGGACCATAA TGCAAGAGAA CATATCCACC
    AGTCAGATGA ACAGAAACTT GAAAAACCGA ATGAATGCAA ATTTTCTGAG TGGCTTAATA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38977
    Clone ID Related Accession (Same CDS sequence) XM_017008318.2 , XM_005263077.3 , XM_017008318.1 , NM_001371398.1 , XM_017008317.1 , XM_005263077.4 , XM_017008317.2 , NM_001371397.1
    Accession Version XM_005263077.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2403bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005263077.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GATCACATGT ACATGAAGAC AATGGAAAGT
    TATTTCCTTC ATCCAGTCTA CAAATACCCA AGGACCATAA TGCAAGAGAA CATATCCACC
    AGTCAGATGA ACAGAAACTT GAAAAACCGA ATGAATGCAA ATTTTCTGAG TGGCTTAATA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005263134.1
    CDS1106..3508
    Translation

    Target ORF information:

    RefSeq Version XM_005263077.3
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005263077.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GATCACATGT ACATGAAGAC AATGGAAAGT
    TATTTCCTTC ATCCAGTCTA CAAATACCCA AGGACCATAA TGCAAGAGAA CATATCCACC
    AGTCAGATGA ACAGAAACTT GAAAAACCGA ATGAATGCAA ATTTTCTGAG TGGCTTAATA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38977
    Clone ID Related Accession (Same CDS sequence) XM_017008318.2 , XM_005263077.3 , XM_017008318.1 , NM_001371398.1 , XM_017008317.1 , XM_005263077.4 , XM_017008317.2 , NM_001371397.1
    Accession Version XM_017008318.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2403bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GATCACATGT ACATGAAGAC AATGGAAAGT
    TATTTCCTTC ATCCAGTCTA CAAATACCCA AGGACCATAA TGCAAGAGAA CATATCCACC
    AGTCAGATGA ACAGAAACTT GAAAAACCGA ATGAATGCAA ATTTTCTGAG TGGCTTAATA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863807.1
    CDS1052..3454
    Translation

    Target ORF information:

    RefSeq Version XM_017008318.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008318.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GATCACATGT ACATGAAGAC AATGGAAAGT
    TATTTCCTTC ATCCAGTCTA CAAATACCCA AGGACCATAA TGCAAGAGAA CATATCCACC
    AGTCAGATGA ACAGAAACTT GAAAAACCGA ATGAATGCAA ATTTTCTGAG TGGCTTAATA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38977
    Clone ID Related Accession (Same CDS sequence) XM_017008318.2 , XM_005263077.3 , XM_017008318.1 , NM_001371398.1 , XM_017008317.1 , XM_005263077.4 , XM_017008317.2 , NM_001371397.1
    Accession Version XM_017008317.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2403bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GATCACATGT ACATGAAGAC AATGGAAAGT
    TATTTCCTTC ATCCAGTCTA CAAATACCCA AGGACCATAA TGCAAGAGAA CATATCCACC
    AGTCAGATGA ACAGAAACTT GAAAAACCGA ATGAATGCAA ATTTTCTGAG TGGCTTAATA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863806.1
    CDS930..3332
    Translation

    Target ORF information:

    RefSeq Version XM_017008317.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008317.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GATCACATGT ACATGAAGAC AATGGAAAGT
    TATTTCCTTC ATCCAGTCTA CAAATACCCA AGGACCATAA TGCAAGAGAA CATATCCACC
    AGTCAGATGA ACAGAAACTT GAAAAACCGA ATGAATGCAA ATTTTCTGAG TGGCTTAATA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38977
    Clone ID Related Accession (Same CDS sequence) XM_017008318.2 , XM_005263077.3 , XM_017008318.1 , NM_001371398.1 , XM_017008317.1 , XM_005263077.4 , XM_017008317.2 , NM_001371397.1
    Accession Version XM_005263077.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2403bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005263077.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GATCACATGT ACATGAAGAC AATGGAAAGT
    TATTTCCTTC ATCCAGTCTA CAAATACCCA AGGACCATAA TGCAAGAGAA CATATCCACC
    AGTCAGATGA ACAGAAACTT GAAAAACCGA ATGAATGCAA ATTTTCTGAG TGGCTTAATA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005263134.1
    CDS1067..3469
    Translation

    Target ORF information:

    RefSeq Version XM_005263077.4
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005263077.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GATCACATGT ACATGAAGAC AATGGAAAGT
    TATTTCCTTC ATCCAGTCTA CAAATACCCA AGGACCATAA TGCAAGAGAA CATATCCACC
    AGTCAGATGA ACAGAAACTT GAAAAACCGA ATGAATGCAA ATTTTCTGAG TGGCTTAATA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38977
    Clone ID Related Accession (Same CDS sequence) XM_017008318.2 , XM_005263077.3 , XM_017008318.1 , NM_001371398.1 , XM_017008317.1 , XM_005263077.4 , XM_017008317.2 , NM_001371397.1
    Accession Version XM_017008317.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2403bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-12
    Organism Homo sapiens(human)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017008317.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GATCACATGT ACATGAAGAC AATGGAAAGT
    TATTTCCTTC ATCCAGTCTA CAAATACCCA AGGACCATAA TGCAAGAGAA CATATCCACC
    AGTCAGATGA ACAGAAACTT GAAAAACCGA ATGAATGCAA ATTTTCTGAG TGGCTTAATA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863806.1
    CDS899..3301
    Translation

    Target ORF information:

    RefSeq Version XM_017008317.2
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008317.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GATCACATGT ACATGAAGAC AATGGAAAGT
    TATTTCCTTC ATCCAGTCTA CAAATACCCA AGGACCATAA TGCAAGAGAA CATATCCACC
    AGTCAGATGA ACAGAAACTT GAAAAACCGA ATGAATGCAA ATTTTCTGAG TGGCTTAATA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38977
    Clone ID Related Accession (Same CDS sequence) XM_017008318.2 , XM_005263077.3 , XM_017008318.1 , NM_001371398.1 , XM_017008317.1 , XM_005263077.4 , XM_017008317.2 , NM_001371397.1
    Accession Version NM_001371398.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2403bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-10
    Organism Homo sapiens(human)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092656.1. On Jul 11, 2019 this sequence version replaced XM_017008318.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.29668.1 [ECO:0000332] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GATCACATGT ACATGAAGAC AATGGAAAGT
    TATTTCCTTC ATCCAGTCTA CAAATACCCA AGGACCATAA TGCAAGAGAA CATATCCACC
    AGTCAGATGA ACAGAAACTT GAAAAACCGA ATGAATGCAA ATTTTCTGAG TGGCTTAATA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001358327.1
    CDS950..3352
    Translation

    Target ORF information:

    RefSeq Version NM_001371398.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001371398.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GATCACATGT ACATGAAGAC AATGGAAAGT
    TATTTCCTTC ATCCAGTCTA CAAATACCCA AGGACCATAA TGCAAGAGAA CATATCCACC
    AGTCAGATGA ACAGAAACTT GAAAAACCGA ATGAATGCAA ATTTTCTGAG TGGCTTAATA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38977
    Clone ID Related Accession (Same CDS sequence) XM_017008318.2 , XM_005263077.3 , XM_017008318.1 , NM_001371398.1 , XM_017008317.1 , XM_005263077.4 , XM_017008317.2 , NM_001371397.1
    Accession Version NM_001371397.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2403bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-10
    Organism Homo sapiens(human)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092656.1. On Jul 11, 2019 this sequence version replaced XM_017008317.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.80818.1 [ECO:0000332] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GATCACATGT ACATGAAGAC AATGGAAAGT
    TATTTCCTTC ATCCAGTCTA CAAATACCCA AGGACCATAA TGCAAGAGAA CATATCCACC
    AGTCAGATGA ACAGAAACTT GAAAAACCGA ATGAATGCAA ATTTTCTGAG TGGCTTAATA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001358326.1
    CDS828..3230
    Translation

    Target ORF information:

    RefSeq Version NM_001371397.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001371397.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GATCACATGT ACATGAAGAC AATGGAAAGT
    TATTTCCTTC ATCCAGTCTA CAAATACCCA AGGACCATAA TGCAAGAGAA CATATCCACC
    AGTCAGATGA ACAGAAACTT GAAAAACCGA ATGAATGCAA ATTTTCTGAG TGGCTTAATA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02420
    Clone ID Related Accession (Same CDS sequence) XM_017008312.2 , XM_005263073.4 , XM_005263073.3 , NM_001371395.1 , XM_017008313.1 , XM_011532037.2 , XM_017008314.1 , XM_017008312.1 , XM_017008315.1 , NM_001371399.1 , NM_019050.2 , XM_017008315.2
    Accession Version NM_001371395.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-10
    Organism Homo sapiens(human)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092656.1. On Jul 11, 2019 this sequence version replaced XM_017008312.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: CR749490.1, SRR1660803.241937.1 [ECO:0000332] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA
    GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG
    ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA
    ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA
    AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT
    TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT
    TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC
    CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA
    ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA
    CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC
    GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG
    CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA
    AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC
    AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA
    GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA
    GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT
    TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001358324.1
    CDS1103..4324
    Translation

    Target ORF information:

    RefSeq Version NM_001371395.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001371395.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA
    GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG
    ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA
    ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA
    AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT
    TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT
    TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC
    CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA
    ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA
    CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC
    GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG
    CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA
    AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC
    AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA
    GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA
    GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT
    TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02420
    Clone ID Related Accession (Same CDS sequence) XM_017008312.2 , XM_005263073.4 , XM_005263073.3 , NM_001371395.1 , XM_017008313.1 , XM_011532037.2 , XM_017008314.1 , XM_017008312.1 , XM_017008315.1 , NM_001371399.1 , NM_019050.2 , XM_017008315.2
    Accession Version NM_001371399.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-10
    Organism Homo sapiens(human)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092656.1. On Jul 11, 2019 this sequence version replaced XM_005263073.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.100920.1, SRR1803611.239951.1 [ECO:0000332] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA
    GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG
    ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA
    ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA
    AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT
    TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT
    TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC
    CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA
    ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA
    CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC
    GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG
    CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA
    AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC
    AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA
    GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA
    GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT
    TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001358328.1
    CDS828..4049
    Translation

    Target ORF information:

    RefSeq Version NM_001371399.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001371399.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA
    GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG
    ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA
    ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA
    AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT
    TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT
    TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC
    CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA
    ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA
    CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC
    GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG
    CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA
    AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC
    AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA
    GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA
    GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT
    TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38975
    Clone ID Related Accession (Same CDS sequence) NM_001371396.1 , XM_005263076.2 , XM_017008316.1 , XM_017008316.2 , XM_005263076.3
    Accession Version NM_001371396.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3069bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-10
    Organism Homo sapiens(human)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092656.1. On Jul 11, 2019 this sequence version replaced XM_017008316.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.74097.1 [ECO:0000332] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGCTAAG
    TTAAGTCACA TTGATCAAAG GGAAAAGATA AAAGACATTT CCAGAGAATG TGCTCTGAAA
    GCTATTGAAC AGAAAAACTT ACTTTCTTCA CAAAGGAAAG ATTTAGAGAA GGGACAAAGA
    AAAGATTTAG GACGACATAG AGATTTGGTT GATGAAGACC TTTCACATTT CCAATCTGGA
    TCACCTCCTG CCCCAAATGG TTTTAAACAA CATGGGAATC CACATCTATA TCATAGTCAA
    GGAAAAGGAT CATATAAACA TGACCGAGTT GTACCTCAGA GTCGAGCTTC TGCACAAATA
    ATAAGTTCAA GTAAATCCCA GATTCTTGCT CCAGGAGAGA AAATAACTGG CAAAGTTAAG
    AGTGACAATG GCACTGGATA TGACACAGAC AGCAGCCAAG ATTCTAGGGA TAGAGGAAAC
    AGCTGTGATA GCAGCAGTAA AAGCCGGAAC CGAGGTTGGA AACCTATGAG AGAAACATTA
    AATGTTGATA GTATTTTTAG TGAAAGTGAA AAAAGACAGC ATAGTCCAAG ACATAAACCA
    AATATCAGTA ATAAGCCTAA ATCTAGCAAG GATCCGAGTT TTAGTAATTG GCCAAAAGAG
    AATCCAAAGC AAAAAGGTTT AATGACCATA TATGAAGATG AAATGAAGCA GGAAATAGGA
    AGCAGAAGTT CCCTTGAATC TAATGGAAAA GGAGCAGAGA AAAATAAAGG CCTTGTAGAG
    GGTAAAGTGC ATGGTGATAA TTGGCAGATG CAAAGGACTG AGTCTGGATA TGAAAGCAGT
    GATCACATCA GTAATGGTTC TACTAATTTG GACTCACCTG TTATCGATGG AAATGGTACA
    GTAATGGATA TCAGTGGTGT TAAAGAAACA GTATGCTTCA GTGACCAGAT TACGACAAGC
    AACCTAAATA AAGAACGTGG GGACTGTACC TCCCTTCAGA GCCAACATCA CTTAGAAGGC
    TTTAGAAAAG AACTCAGGAA TTTGGAAGCA GGCTATAAAT CTCATGAATT CCACCCAGAA
    TCACATTTAC AAATAAAAAA TCATTTGATA AAAAGATCAC ATGTACATGA AGACAATGGA
    AAGTTATTTC CTTCATCCAG TCTACAAATA CCCAAGGACC ATAATGCAAG AGAACATATC
    CACCAGTCAG ATGAACAGAA ACTTGAAAAA CCGAATGAAT GCAAATTTTC TGAGTGGCTT
    AATATAGAAA ATTCTGAGAG AACAGGTTTG CCTTTTCACG TTGATAACTC TGCTTCTGGG
    AAGAGAGTGA ACAGTAATGA ACCATCTTCA TTATGGTCTT CACACCTAAG AACTGTTGGG
    TTAAAGCCAG AAACTGCTCC TCTCATCCAG CAACAAAATA TCATGGATCA ATGTTACTTT
    GAGAACTCTC TATCCACAGA ATGTATAATT CGGTCAGCCA GCAGATCTGA TGGGTGTCAG
    ATGCCAAAAC TTTTTTGCCA GAATCTACCA CCCCCTTTGC CACCAAAGAA ATATGCTATA
    ACCAGTGTGC CACAGTCAGA GAAAAGCGAA TCTACACCTG ATGTCAAACT TACAGAGGTG
    TTTAAAGCTA CCTCTCATCT TCCGAAGCAC AGTTTAAGTA CAGCTTCAGA ACCAAGTTTA
    GAAGTGAGTA CACATATGAA TGATGAAAGA CATAAAGAAA CATTTCAAGT GAGAGAATGT
    TTTGGCAACA CACCAAACTG TCCATCCAGC TCCTCAACTA ACGATTTTCA GGCAAACTCA
    GGTGCCATTG ATGCATTTTG CCAACCAGAA CTAGACTCTA TTTCTACCTG TCCAAATGAG
    ACAGTTTCAT TAACTACCTA TTTTTCAGTT GATAGCTGCA TGACGGATAC ATATAGATTG
    AAATACCATC AGAGGCCCAA GCTCTCTTTT CCAGAGAGCA GTGGCTTTTG TAATAATTCA
    CTATCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001358325.1
    CDS828..3896
    Translation

    Target ORF information:

    RefSeq Version NM_001371396.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001371396.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGCTAAG
    TTAAGTCACA TTGATCAAAG GGAAAAGATA AAAGACATTT CCAGAGAATG TGCTCTGAAA
    GCTATTGAAC AGAAAAACTT ACTTTCTTCA CAAAGGAAAG ATTTAGAGAA GGGACAAAGA
    AAAGATTTAG GACGACATAG AGATTTGGTT GATGAAGACC TTTCACATTT CCAATCTGGA
    TCACCTCCTG CCCCAAATGG TTTTAAACAA CATGGGAATC CACATCTATA TCATAGTCAA
    GGAAAAGGAT CATATAAACA TGACCGAGTT GTACCTCAGA GTCGAGCTTC TGCACAAATA
    ATAAGTTCAA GTAAATCCCA GATTCTTGCT CCAGGAGAGA AAATAACTGG CAAAGTTAAG
    AGTGACAATG GCACTGGATA TGACACAGAC AGCAGCCAAG ATTCTAGGGA TAGAGGAAAC
    AGCTGTGATA GCAGCAGTAA AAGCCGGAAC CGAGGTTGGA AACCTATGAG AGAAACATTA
    AATGTTGATA GTATTTTTAG TGAAAGTGAA AAAAGACAGC ATAGTCCAAG ACATAAACCA
    AATATCAGTA ATAAGCCTAA ATCTAGCAAG GATCCGAGTT TTAGTAATTG GCCAAAAGAG
    AATCCAAAGC AAAAAGGTTT AATGACCATA TATGAAGATG AAATGAAGCA GGAAATAGGA
    AGCAGAAGTT CCCTTGAATC TAATGGAAAA GGAGCAGAGA AAAATAAAGG CCTTGTAGAG
    GGTAAAGTGC ATGGTGATAA TTGGCAGATG CAAAGGACTG AGTCTGGATA TGAAAGCAGT
    GATCACATCA GTAATGGTTC TACTAATTTG GACTCACCTG TTATCGATGG AAATGGTACA
    GTAATGGATA TCAGTGGTGT TAAAGAAACA GTATGCTTCA GTGACCAGAT TACGACAAGC
    AACCTAAATA AAGAACGTGG GGACTGTACC TCCCTTCAGA GCCAACATCA CTTAGAAGGC
    TTTAGAAAAG AACTCAGGAA TTTGGAAGCA GGCTATAAAT CTCATGAATT CCACCCAGAA
    TCACATTTAC AAATAAAAAA TCATTTGATA AAAAGATCAC ATGTACATGA AGACAATGGA
    AAGTTATTTC CTTCATCCAG TCTACAAATA CCCAAGGACC ATAATGCAAG AGAACATATC
    CACCAGTCAG ATGAACAGAA ACTTGAAAAA CCGAATGAAT GCAAATTTTC TGAGTGGCTT
    AATATAGAAA ATTCTGAGAG AACAGGTTTG CCTTTTCACG TTGATAACTC TGCTTCTGGG
    AAGAGAGTGA ACAGTAATGA ACCATCTTCA TTATGGTCTT CACACCTAAG AACTGTTGGG
    TTAAAGCCAG AAACTGCTCC TCTCATCCAG CAACAAAATA TCATGGATCA ATGTTACTTT
    GAGAACTCTC TATCCACAGA ATGTATAATT CGGTCAGCCA GCAGATCTGA TGGGTGTCAG
    ATGCCAAAAC TTTTTTGCCA GAATCTACCA CCCCCTTTGC CACCAAAGAA ATATGCTATA
    ACCAGTGTGC CACAGTCAGA GAAAAGCGAA TCTACACCTG ATGTCAAACT TACAGAGGTG
    TTTAAAGCTA CCTCTCATCT TCCGAAGCAC AGTTTAAGTA CAGCTTCAGA ACCAAGTTTA
    GAAGTGAGTA CACATATGAA TGATGAAAGA CATAAAGAAA CATTTCAAGT GAGAGAATGT
    TTTGGCAACA CACCAAACTG TCCATCCAGC TCCTCAACTA ACGATTTTCA GGCAAACTCA
    GGTGCCATTG ATGCATTTTG CCAACCAGAA CTAGACTCTA TTTCTACCTG TCCAAATGAG
    ACAGTTTCAT TAACTACCTA TTTTTCAGTT GATAGCTGCA TGACGGATAC ATATAGATTG
    AAATACCATC AGAGGCCCAA GCTCTCTTTT CCAGAGAGCA GTGGCTTTTG TAATAATTCA
    CTATCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02420
    Clone ID Related Accession (Same CDS sequence) XM_017008312.2 , XM_005263073.4 , XM_005263073.3 , NM_001371395.1 , XM_017008313.1 , XM_011532037.2 , XM_017008314.1 , XM_017008312.1 , XM_017008315.1 , NM_001371399.1 , NM_019050.2 , XM_017008315.2
    Accession Version NM_019050.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-20
    Organism Homo sapiens(human)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA886370.1, DA859085.1, CR749490.1, AB037771.1, BX648339.1, AK022799.1, CD721951.1 and BQ019723.1. On Jun 2, 2007 this sequence version replaced NM_019050.1. ##Evidence-Data-START## CDS exon combination :: SRR1660807.47065.1, SRR1660803.100920.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA
    GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG
    ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA
    ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA
    AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT
    TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT
    TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC
    CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA
    ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA
    CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC
    GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG
    CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA
    AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC
    AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA
    GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA
    GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT
    TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_061923.2
    CDS1067..4288
    Translation

    Target ORF information:

    RefSeq Version NM_019050.2
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_019050.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA
    GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG
    ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA
    ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA
    AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT
    TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT
    TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC
    CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA
    ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA
    CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC
    GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG
    CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA
    AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC
    AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA
    GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA
    GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT
    TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02420
    Clone ID Related Accession (Same CDS sequence) XM_017008312.2 , XM_005263073.4 , XM_005263073.3 , NM_001371395.1 , XM_017008313.1 , XM_011532037.2 , XM_017008314.1 , XM_017008312.1 , XM_017008315.1 , NM_001371399.1 , NM_019050.2 , XM_017008315.2
    Accession Version XM_017008312.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-12
    Organism Homo sapiens(human)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017008312.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA
    GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG
    ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA
    ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA
    AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT
    TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT
    TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC
    CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA
    ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA
    CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC
    GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG
    CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA
    AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC
    AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA
    GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA
    GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT
    TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863801.1
    CDS1233..4454
    Translation

    Target ORF information:

    RefSeq Version XM_017008312.2
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008312.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA
    GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG
    ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA
    ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA
    AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT
    TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT
    TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC
    CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA
    ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA
    CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC
    GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG
    CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA
    AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC
    AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA
    GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA
    GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT
    TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02420
    Clone ID Related Accession (Same CDS sequence) XM_017008312.2 , XM_005263073.4 , XM_005263073.3 , NM_001371395.1 , XM_017008313.1 , XM_011532037.2 , XM_017008314.1 , XM_017008312.1 , XM_017008315.1 , NM_001371399.1 , NM_019050.2 , XM_017008315.2
    Accession Version XM_005263073.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-12
    Organism Homo sapiens(human)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005263073.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA
    GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG
    ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA
    ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA
    AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT
    TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT
    TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC
    CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA
    ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA
    CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC
    GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG
    CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA
    AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC
    AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA
    GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA
    GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT
    TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005263130.1
    CDS899..4120
    Translation

    Target ORF information:

    RefSeq Version XM_005263073.4
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005263073.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA
    GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG
    ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA
    ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA
    AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT
    TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT
    TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC
    CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA
    ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA
    CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC
    GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG
    CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA
    AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC
    AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA
    GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA
    GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT
    TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02420
    Clone ID Related Accession (Same CDS sequence) XM_017008312.2 , XM_005263073.4 , XM_005263073.3 , NM_001371395.1 , XM_017008313.1 , XM_011532037.2 , XM_017008314.1 , XM_017008312.1 , XM_017008315.1 , NM_001371399.1 , NM_019050.2 , XM_017008315.2
    Accession Version XM_005263073.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005263073.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA
    GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG
    ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA
    ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA
    AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT
    TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT
    TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC
    CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA
    ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA
    CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC
    GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG
    CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA
    AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC
    AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA
    GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA
    GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT
    TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005263130.1
    CDS941..4162
    Translation

    Target ORF information:

    RefSeq Version XM_005263073.3
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005263073.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA
    GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG
    ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA
    ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA
    AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT
    TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT
    TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC
    CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA
    ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA
    CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC
    GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG
    CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA
    AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC
    AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA
    GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA
    GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT
    TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02420
    Clone ID Related Accession (Same CDS sequence) XM_017008312.2 , XM_005263073.4 , XM_005263073.3 , NM_001371395.1 , XM_017008313.1 , XM_011532037.2 , XM_017008314.1 , XM_017008312.1 , XM_017008315.1 , NM_001371399.1 , NM_019050.2 , XM_017008315.2
    Accession Version XM_017008313.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA
    GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG
    ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA
    ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA
    AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT
    TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT
    TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC
    CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA
    ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA
    CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC
    GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG
    CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA
    AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC
    AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA
    GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA
    GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT
    TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863802.1
    CDS918..4139
    Translation

    Target ORF information:

    RefSeq Version XM_017008313.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008313.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA
    GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG
    ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA
    ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA
    AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT
    TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT
    TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC
    CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA
    ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA
    CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC
    GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG
    CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA
    AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC
    AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA
    GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA
    GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT
    TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02420
    Clone ID Related Accession (Same CDS sequence) XM_017008312.2 , XM_005263073.4 , XM_005263073.3 , NM_001371395.1 , XM_017008313.1 , XM_011532037.2 , XM_017008314.1 , XM_017008312.1 , XM_017008315.1 , NM_001371399.1 , NM_019050.2 , XM_017008315.2
    Accession Version XM_011532037.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011532037.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA
    GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG
    ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA
    ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA
    AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT
    TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT
    TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC
    CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA
    ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA
    CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC
    GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG
    CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA
    AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC
    AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA
    GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA
    GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT
    TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011530339.1
    CDS1119..4340
    Translation

    Target ORF information:

    RefSeq Version XM_011532037.2
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011532037.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA
    GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG
    ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA
    ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA
    AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT
    TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT
    TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC
    CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA
    ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA
    CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC
    GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG
    CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA
    AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC
    AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA
    GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA
    GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT
    TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02420
    Clone ID Related Accession (Same CDS sequence) XM_017008312.2 , XM_005263073.4 , XM_005263073.3 , NM_001371395.1 , XM_017008313.1 , XM_011532037.2 , XM_017008314.1 , XM_017008312.1 , XM_017008315.1 , NM_001371399.1 , NM_019050.2 , XM_017008315.2
    Accession Version XM_017008314.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA
    GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG
    ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA
    ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA
    AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT
    TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT
    TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC
    CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA
    ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA
    CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC
    GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG
    CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA
    AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC
    AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA
    GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA
    GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT
    TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863803.1
    CDS917..4138
    Translation

    Target ORF information:

    RefSeq Version XM_017008314.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008314.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA
    GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG
    ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA
    ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA
    AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT
    TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT
    TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC
    CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA
    ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA
    CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC
    GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG
    CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA
    AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC
    AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA
    GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA
    GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT
    TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02420
    Clone ID Related Accession (Same CDS sequence) XM_017008312.2 , XM_005263073.4 , XM_005263073.3 , NM_001371395.1 , XM_017008313.1 , XM_011532037.2 , XM_017008314.1 , XM_017008312.1 , XM_017008315.1 , NM_001371399.1 , NM_019050.2 , XM_017008315.2
    Accession Version XM_017008312.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA
    GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG
    ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA
    ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA
    AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT
    TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT
    TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC
    CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA
    ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA
    CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC
    GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG
    CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA
    AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC
    AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA
    GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA
    GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT
    TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863801.1
    CDS1233..4454
    Translation

    Target ORF information:

    RefSeq Version XM_017008312.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008312.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA
    GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG
    ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA
    ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA
    AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT
    TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT
    TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC
    CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA
    ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA
    CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC
    GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG
    CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA
    AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC
    AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA
    GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA
    GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT
    TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02420
    Clone ID Related Accession (Same CDS sequence) XM_017008312.2 , XM_005263073.4 , XM_005263073.3 , NM_001371395.1 , XM_017008313.1 , XM_011532037.2 , XM_017008314.1 , XM_017008312.1 , XM_017008315.1 , NM_001371399.1 , NM_019050.2 , XM_017008315.2
    Accession Version XM_017008315.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA
    GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG
    ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA
    ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA
    AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT
    TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT
    TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC
    CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA
    ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA
    CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC
    GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG
    CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA
    AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC
    AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA
    GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA
    GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT
    TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863804.1
    CDS987..4208
    Translation

    Target ORF information:

    RefSeq Version XM_017008315.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008315.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA
    GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG
    ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA
    ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA
    AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT
    TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT
    TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC
    CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA
    ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA
    CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC
    GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG
    CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA
    AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC
    AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA
    GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA
    GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT
    TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02420
    Clone ID Related Accession (Same CDS sequence) XM_017008312.2 , XM_005263073.4 , XM_005263073.3 , NM_001371395.1 , XM_017008313.1 , XM_011532037.2 , XM_017008314.1 , XM_017008312.1 , XM_017008315.1 , NM_001371399.1 , NM_019050.2 , XM_017008315.2
    Accession Version XM_017008315.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017008315.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA
    GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG
    ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA
    ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA
    AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT
    TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT
    TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC
    CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA
    ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA
    CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC
    GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG
    CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA
    AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC
    AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA
    GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA
    GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT
    TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863804.1
    CDS1115..4336
    Translation

    Target ORF information:

    RefSeq Version XM_017008315.2
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008315.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT
    GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA
    AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC
    AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG
    ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT
    TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG
    GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA
    CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA
    GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT
    GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA
    GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG
    AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT
    GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC
    AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC
    ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA
    AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG
    ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT
    TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA
    ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT
    ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA
    GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG
    ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA
    ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA
    AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT
    TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT
    TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC
    CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA
    ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA
    CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC
    GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG
    CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA
    AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC
    AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA
    GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA
    GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT
    TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38974
    Clone ID Related Accession (Same CDS sequence) XM_005263075.3 , XM_005263075.2
    Accession Version XM_005263075.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3072bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005263075.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGATTGGAAC TAGATGGAAA
    GATGTTGTCT CCAAATGCAT TCGATGCCAC TTTCAGCCAC TACTTTTGTT TTATGCAAAC
    CCAGATGGCA CAGCAGTTTC TACTGAGGAT GCACTCAGGC AGGTCATCAG CTGGTCACAT
    TACAAATCTG TTGCAGAAAA TATGGGATGT GAAAAGCCTG TAATTCATAA GTCAGATAAT
    TTAAAAGAAA ATGGATTTGG TGATCAGGCA AAGCAGAGAG AAAATCAGAA ATTTCCAACT
    GATAATATTT CATCATCTAA TCGGAGCCAC AGTCACACAG GTGTAGGGAA AGGACCAGCT
    AAGTTAAGTC ACATTGATCA AAGGGAAAAG ATAAAAGACA TTTCCAGAGA ATGTGCTCTG
    AAAGCTATTG AACAGAAAAA CTTACTTTCT TCACAAAGGA AAGATTTAGA GAAGGGACAA
    AGAAAAGATT TAGGACGACA TAGAGATTTG GTTGATGAAG ACCTTTCACA TTTCCAATCT
    GGATCACCTC CTGCCCCAAA TGGTTTTAAA CAACATGGGA ATCCACATCT ATATCATAGT
    CAAGGAAAAG GATCATATAA ACATGACCGA GTTGTACCTC AGAGTCGAGC TTCTGCACAA
    ATAATAAGTT CAAGTAAATC CCAGATTCTT GCTCCAGGAG AGAAAATAAC TGGCAAAGTT
    AAGAGTGACA ATGGCACTGG ATATGACACA GACAGCAGCC AAGATTCTAG GGATAGAGGA
    AACAGCTGTG ATAGCAGCAG TAAAAGCCGG AACCGAGGTT GGAAACCTAT GAGAGAAACA
    TTAAATGTTG ATAGTATTTT TAGTGAAAGT GAAAAAAGAC AGCATAGTCC AAGACATAAA
    CCAAATATCA GTAATAAGCC TAAATCTAGC AAGGATCCGA GTTTTAGTAA TTGGCCAAAA
    GAGAATCCAA AGCAAAAAGG TTTAATGACC ATATATGAAG ATGAAATGAA GCAGGAAATA
    GGAAGCAGAA GTTCCCTTGA ATCTAATGGA AAAGGAGCAG AGAAAAATAA AGGCCTTGTA
    GAGGGTAAAG TGCATGGTGA TAATTGGCAG ATGCAAAGGA CTGAGTCTGG ATATGAAAGC
    AGTGATCACA TCAGTAATGG TTCTACTAAT TTGGACTCAC CTGTTATCGA TGGAAATGGT
    ACAGTAATGG ATATCAGTGG TGTTAAAGAA ACAGTATGCT TCAGTGACCA GATTACGACA
    AGCAACCTAA ATAAAGAACG TGGGGACTGT ACCTCCCTTC AGAGCCAACA TCACTTAGAA
    GGCTTTAGAA AAGAACTCAG GAATTTGGAA GCAGGCTATA AATCTCATGA ATTCCACCCA
    GAATCACATT TACAAATAAA AAATCATTTG ATAAAAAGAT CACATGTACA TGAAGACAAT
    GGAAAGTTAT TTCCTTCATC CAGTCTACAA ATACCCAAGG ACCATAATGC AAGAGAACAT
    ATCCACCAGT CAGATGAACA GAAACTTGAA AAACCGAATG AATGCAAATT TTCTGAGTGG
    CTTAATATAG AAAATTCTGA GAGAACAGGT TTGCCTTTTC ACGTTGATAA CTCTGCTTCT
    GGGAAGAGAG TGAACAGTAA TGAACCATCT TCATTATGGT CTTCACACCT AAGAACTGTT
    GGGTTAAAGC CAGAAACTGC TCCTCTCATC CAGCAACAAA ATATCATGGA TCAATGTTAC
    TTTGAGAACT CTCTATCCAC AGAATGTATA ATTCGGTCAG CCAGCAGATC TGATGGGTGT
    CAGATGCCAA AACTTTTTTG CCAGAATCTA CCACCCCCTT TGCCACCAAA GAAATATGCT
    ATAACCAGTG TGCCACAGTC AGAGAAAAGC GAATCTACAC CTGATGTCAA ACTTACAGAG
    GTGTTTAAAG CTACCTCTCA TCTTCCGAAG CACAGTTTAA GTACAGCTTC AGAACCAAGT
    TTAGAAGTGA GTACACATAT GAATGATGAA AGACATAAAG AAACATTTCA AGTGAGAGAA
    TGTTTTGGCA ACACACCAAA CTGTCCATCC AGCTCCTCAA CTAACGATTT TCAGGCAAAC
    TCAGGTGCCA TTGATGCATT TTGCCAACCA GAACTAGACT CTATTTCTAC CTGTCCAAAT
    GAGACAGTTT CATTAACTAC CTATTTTTCA GTTGATAGCT GCATGACGGA TACATATAGA
    TTGAAATACC ATCAGAGGCC CAAGCTCTCT TTTCCAGAGA GCAGTGGCTT TTGTAATAAT
    TCACTATCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005263132.1
    CDS1067..4138
    Translation

    Target ORF information:

    RefSeq Version XM_005263075.3
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005263075.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGATTGGAAC TAGATGGAAA
    GATGTTGTCT CCAAATGCAT TCGATGCCAC TTTCAGCCAC TACTTTTGTT TTATGCAAAC
    CCAGATGGCA CAGCAGTTTC TACTGAGGAT GCACTCAGGC AGGTCATCAG CTGGTCACAT
    TACAAATCTG TTGCAGAAAA TATGGGATGT GAAAAGCCTG TAATTCATAA GTCAGATAAT
    TTAAAAGAAA ATGGATTTGG TGATCAGGCA AAGCAGAGAG AAAATCAGAA ATTTCCAACT
    GATAATATTT CATCATCTAA TCGGAGCCAC AGTCACACAG GTGTAGGGAA AGGACCAGCT
    AAGTTAAGTC ACATTGATCA AAGGGAAAAG ATAAAAGACA TTTCCAGAGA ATGTGCTCTG
    AAAGCTATTG AACAGAAAAA CTTACTTTCT TCACAAAGGA AAGATTTAGA GAAGGGACAA
    AGAAAAGATT TAGGACGACA TAGAGATTTG GTTGATGAAG ACCTTTCACA TTTCCAATCT
    GGATCACCTC CTGCCCCAAA TGGTTTTAAA CAACATGGGA ATCCACATCT ATATCATAGT
    CAAGGAAAAG GATCATATAA ACATGACCGA GTTGTACCTC AGAGTCGAGC TTCTGCACAA
    ATAATAAGTT CAAGTAAATC CCAGATTCTT GCTCCAGGAG AGAAAATAAC TGGCAAAGTT
    AAGAGTGACA ATGGCACTGG ATATGACACA GACAGCAGCC AAGATTCTAG GGATAGAGGA
    AACAGCTGTG ATAGCAGCAG TAAAAGCCGG AACCGAGGTT GGAAACCTAT GAGAGAAACA
    TTAAATGTTG ATAGTATTTT TAGTGAAAGT GAAAAAAGAC AGCATAGTCC AAGACATAAA
    CCAAATATCA GTAATAAGCC TAAATCTAGC AAGGATCCGA GTTTTAGTAA TTGGCCAAAA
    GAGAATCCAA AGCAAAAAGG TTTAATGACC ATATATGAAG ATGAAATGAA GCAGGAAATA
    GGAAGCAGAA GTTCCCTTGA ATCTAATGGA AAAGGAGCAG AGAAAAATAA AGGCCTTGTA
    GAGGGTAAAG TGCATGGTGA TAATTGGCAG ATGCAAAGGA CTGAGTCTGG ATATGAAAGC
    AGTGATCACA TCAGTAATGG TTCTACTAAT TTGGACTCAC CTGTTATCGA TGGAAATGGT
    ACAGTAATGG ATATCAGTGG TGTTAAAGAA ACAGTATGCT TCAGTGACCA GATTACGACA
    AGCAACCTAA ATAAAGAACG TGGGGACTGT ACCTCCCTTC AGAGCCAACA TCACTTAGAA
    GGCTTTAGAA AAGAACTCAG GAATTTGGAA GCAGGCTATA AATCTCATGA ATTCCACCCA
    GAATCACATT TACAAATAAA AAATCATTTG ATAAAAAGAT CACATGTACA TGAAGACAAT
    GGAAAGTTAT TTCCTTCATC CAGTCTACAA ATACCCAAGG ACCATAATGC AAGAGAACAT
    ATCCACCAGT CAGATGAACA GAAACTTGAA AAACCGAATG AATGCAAATT TTCTGAGTGG
    CTTAATATAG AAAATTCTGA GAGAACAGGT TTGCCTTTTC ACGTTGATAA CTCTGCTTCT
    GGGAAGAGAG TGAACAGTAA TGAACCATCT TCATTATGGT CTTCACACCT AAGAACTGTT
    GGGTTAAAGC CAGAAACTGC TCCTCTCATC CAGCAACAAA ATATCATGGA TCAATGTTAC
    TTTGAGAACT CTCTATCCAC AGAATGTATA ATTCGGTCAG CCAGCAGATC TGATGGGTGT
    CAGATGCCAA AACTTTTTTG CCAGAATCTA CCACCCCCTT TGCCACCAAA GAAATATGCT
    ATAACCAGTG TGCCACAGTC AGAGAAAAGC GAATCTACAC CTGATGTCAA ACTTACAGAG
    GTGTTTAAAG CTACCTCTCA TCTTCCGAAG CACAGTTTAA GTACAGCTTC AGAACCAAGT
    TTAGAAGTGA GTACACATAT GAATGATGAA AGACATAAAG AAACATTTCA AGTGAGAGAA
    TGTTTTGGCA ACACACCAAA CTGTCCATCC AGCTCCTCAA CTAACGATTT TCAGGCAAAC
    TCAGGTGCCA TTGATGCATT TTGCCAACCA GAACTAGACT CTATTTCTAC CTGTCCAAAT
    GAGACAGTTT CATTAACTAC CTATTTTTCA GTTGATAGCT GCATGACGGA TACATATAGA
    TTGAAATACC ATCAGAGGCC CAAGCTCTCT TTTCCAGAGA GCAGTGGCTT TTGTAATAAT
    TCACTATCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38974
    Clone ID Related Accession (Same CDS sequence) XM_005263075.3 , XM_005263075.2
    Accession Version XM_005263075.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3072bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005263075.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGATTGGAAC TAGATGGAAA
    GATGTTGTCT CCAAATGCAT TCGATGCCAC TTTCAGCCAC TACTTTTGTT TTATGCAAAC
    CCAGATGGCA CAGCAGTTTC TACTGAGGAT GCACTCAGGC AGGTCATCAG CTGGTCACAT
    TACAAATCTG TTGCAGAAAA TATGGGATGT GAAAAGCCTG TAATTCATAA GTCAGATAAT
    TTAAAAGAAA ATGGATTTGG TGATCAGGCA AAGCAGAGAG AAAATCAGAA ATTTCCAACT
    GATAATATTT CATCATCTAA TCGGAGCCAC AGTCACACAG GTGTAGGGAA AGGACCAGCT
    AAGTTAAGTC ACATTGATCA AAGGGAAAAG ATAAAAGACA TTTCCAGAGA ATGTGCTCTG
    AAAGCTATTG AACAGAAAAA CTTACTTTCT TCACAAAGGA AAGATTTAGA GAAGGGACAA
    AGAAAAGATT TAGGACGACA TAGAGATTTG GTTGATGAAG ACCTTTCACA TTTCCAATCT
    GGATCACCTC CTGCCCCAAA TGGTTTTAAA CAACATGGGA ATCCACATCT ATATCATAGT
    CAAGGAAAAG GATCATATAA ACATGACCGA GTTGTACCTC AGAGTCGAGC TTCTGCACAA
    ATAATAAGTT CAAGTAAATC CCAGATTCTT GCTCCAGGAG AGAAAATAAC TGGCAAAGTT
    AAGAGTGACA ATGGCACTGG ATATGACACA GACAGCAGCC AAGATTCTAG GGATAGAGGA
    AACAGCTGTG ATAGCAGCAG TAAAAGCCGG AACCGAGGTT GGAAACCTAT GAGAGAAACA
    TTAAATGTTG ATAGTATTTT TAGTGAAAGT GAAAAAAGAC AGCATAGTCC AAGACATAAA
    CCAAATATCA GTAATAAGCC TAAATCTAGC AAGGATCCGA GTTTTAGTAA TTGGCCAAAA
    GAGAATCCAA AGCAAAAAGG TTTAATGACC ATATATGAAG ATGAAATGAA GCAGGAAATA
    GGAAGCAGAA GTTCCCTTGA ATCTAATGGA AAAGGAGCAG AGAAAAATAA AGGCCTTGTA
    GAGGGTAAAG TGCATGGTGA TAATTGGCAG ATGCAAAGGA CTGAGTCTGG ATATGAAAGC
    AGTGATCACA TCAGTAATGG TTCTACTAAT TTGGACTCAC CTGTTATCGA TGGAAATGGT
    ACAGTAATGG ATATCAGTGG TGTTAAAGAA ACAGTATGCT TCAGTGACCA GATTACGACA
    AGCAACCTAA ATAAAGAACG TGGGGACTGT ACCTCCCTTC AGAGCCAACA TCACTTAGAA
    GGCTTTAGAA AAGAACTCAG GAATTTGGAA GCAGGCTATA AATCTCATGA ATTCCACCCA
    GAATCACATT TACAAATAAA AAATCATTTG ATAAAAAGAT CACATGTACA TGAAGACAAT
    GGAAAGTTAT TTCCTTCATC CAGTCTACAA ATACCCAAGG ACCATAATGC AAGAGAACAT
    ATCCACCAGT CAGATGAACA GAAACTTGAA AAACCGAATG AATGCAAATT TTCTGAGTGG
    CTTAATATAG AAAATTCTGA GAGAACAGGT TTGCCTTTTC ACGTTGATAA CTCTGCTTCT
    GGGAAGAGAG TGAACAGTAA TGAACCATCT TCATTATGGT CTTCACACCT AAGAACTGTT
    GGGTTAAAGC CAGAAACTGC TCCTCTCATC CAGCAACAAA ATATCATGGA TCAATGTTAC
    TTTGAGAACT CTCTATCCAC AGAATGTATA ATTCGGTCAG CCAGCAGATC TGATGGGTGT
    CAGATGCCAA AACTTTTTTG CCAGAATCTA CCACCCCCTT TGCCACCAAA GAAATATGCT
    ATAACCAGTG TGCCACAGTC AGAGAAAAGC GAATCTACAC CTGATGTCAA ACTTACAGAG
    GTGTTTAAAG CTACCTCTCA TCTTCCGAAG CACAGTTTAA GTACAGCTTC AGAACCAAGT
    TTAGAAGTGA GTACACATAT GAATGATGAA AGACATAAAG AAACATTTCA AGTGAGAGAA
    TGTTTTGGCA ACACACCAAA CTGTCCATCC AGCTCCTCAA CTAACGATTT TCAGGCAAAC
    TCAGGTGCCA TTGATGCATT TTGCCAACCA GAACTAGACT CTATTTCTAC CTGTCCAAAT
    GAGACAGTTT CATTAACTAC CTATTTTTCA GTTGATAGCT GCATGACGGA TACATATAGA
    TTGAAATACC ATCAGAGGCC CAAGCTCTCT TTTCCAGAGA GCAGTGGCTT TTGTAATAAT
    TCACTATCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005263132.1
    CDS1106..4177
    Translation

    Target ORF information:

    RefSeq Version XM_005263075.2
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005263075.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGATTGGAAC TAGATGGAAA
    GATGTTGTCT CCAAATGCAT TCGATGCCAC TTTCAGCCAC TACTTTTGTT TTATGCAAAC
    CCAGATGGCA CAGCAGTTTC TACTGAGGAT GCACTCAGGC AGGTCATCAG CTGGTCACAT
    TACAAATCTG TTGCAGAAAA TATGGGATGT GAAAAGCCTG TAATTCATAA GTCAGATAAT
    TTAAAAGAAA ATGGATTTGG TGATCAGGCA AAGCAGAGAG AAAATCAGAA ATTTCCAACT
    GATAATATTT CATCATCTAA TCGGAGCCAC AGTCACACAG GTGTAGGGAA AGGACCAGCT
    AAGTTAAGTC ACATTGATCA AAGGGAAAAG ATAAAAGACA TTTCCAGAGA ATGTGCTCTG
    AAAGCTATTG AACAGAAAAA CTTACTTTCT TCACAAAGGA AAGATTTAGA GAAGGGACAA
    AGAAAAGATT TAGGACGACA TAGAGATTTG GTTGATGAAG ACCTTTCACA TTTCCAATCT
    GGATCACCTC CTGCCCCAAA TGGTTTTAAA CAACATGGGA ATCCACATCT ATATCATAGT
    CAAGGAAAAG GATCATATAA ACATGACCGA GTTGTACCTC AGAGTCGAGC TTCTGCACAA
    ATAATAAGTT CAAGTAAATC CCAGATTCTT GCTCCAGGAG AGAAAATAAC TGGCAAAGTT
    AAGAGTGACA ATGGCACTGG ATATGACACA GACAGCAGCC AAGATTCTAG GGATAGAGGA
    AACAGCTGTG ATAGCAGCAG TAAAAGCCGG AACCGAGGTT GGAAACCTAT GAGAGAAACA
    TTAAATGTTG ATAGTATTTT TAGTGAAAGT GAAAAAAGAC AGCATAGTCC AAGACATAAA
    CCAAATATCA GTAATAAGCC TAAATCTAGC AAGGATCCGA GTTTTAGTAA TTGGCCAAAA
    GAGAATCCAA AGCAAAAAGG TTTAATGACC ATATATGAAG ATGAAATGAA GCAGGAAATA
    GGAAGCAGAA GTTCCCTTGA ATCTAATGGA AAAGGAGCAG AGAAAAATAA AGGCCTTGTA
    GAGGGTAAAG TGCATGGTGA TAATTGGCAG ATGCAAAGGA CTGAGTCTGG ATATGAAAGC
    AGTGATCACA TCAGTAATGG TTCTACTAAT TTGGACTCAC CTGTTATCGA TGGAAATGGT
    ACAGTAATGG ATATCAGTGG TGTTAAAGAA ACAGTATGCT TCAGTGACCA GATTACGACA
    AGCAACCTAA ATAAAGAACG TGGGGACTGT ACCTCCCTTC AGAGCCAACA TCACTTAGAA
    GGCTTTAGAA AAGAACTCAG GAATTTGGAA GCAGGCTATA AATCTCATGA ATTCCACCCA
    GAATCACATT TACAAATAAA AAATCATTTG ATAAAAAGAT CACATGTACA TGAAGACAAT
    GGAAAGTTAT TTCCTTCATC CAGTCTACAA ATACCCAAGG ACCATAATGC AAGAGAACAT
    ATCCACCAGT CAGATGAACA GAAACTTGAA AAACCGAATG AATGCAAATT TTCTGAGTGG
    CTTAATATAG AAAATTCTGA GAGAACAGGT TTGCCTTTTC ACGTTGATAA CTCTGCTTCT
    GGGAAGAGAG TGAACAGTAA TGAACCATCT TCATTATGGT CTTCACACCT AAGAACTGTT
    GGGTTAAAGC CAGAAACTGC TCCTCTCATC CAGCAACAAA ATATCATGGA TCAATGTTAC
    TTTGAGAACT CTCTATCCAC AGAATGTATA ATTCGGTCAG CCAGCAGATC TGATGGGTGT
    CAGATGCCAA AACTTTTTTG CCAGAATCTA CCACCCCCTT TGCCACCAAA GAAATATGCT
    ATAACCAGTG TGCCACAGTC AGAGAAAAGC GAATCTACAC CTGATGTCAA ACTTACAGAG
    GTGTTTAAAG CTACCTCTCA TCTTCCGAAG CACAGTTTAA GTACAGCTTC AGAACCAAGT
    TTAGAAGTGA GTACACATAT GAATGATGAA AGACATAAAG AAACATTTCA AGTGAGAGAA
    TGTTTTGGCA ACACACCAAA CTGTCCATCC AGCTCCTCAA CTAACGATTT TCAGGCAAAC
    TCAGGTGCCA TTGATGCATT TTGCCAACCA GAACTAGACT CTATTTCTAC CTGTCCAAAT
    GAGACAGTTT CATTAACTAC CTATTTTTCA GTTGATAGCT GCATGACGGA TACATATAGA
    TTGAAATACC ATCAGAGGCC CAAGCTCTCT TTTCCAGAGA GCAGTGGCTT TTGTAATAAT
    TCACTATCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38975
    Clone ID Related Accession (Same CDS sequence) NM_001371396.1 , XM_005263076.2 , XM_017008316.1 , XM_017008316.2 , XM_005263076.3
    Accession Version XM_005263076.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3069bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005263076.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGCTAAG
    TTAAGTCACA TTGATCAAAG GGAAAAGATA AAAGACATTT CCAGAGAATG TGCTCTGAAA
    GCTATTGAAC AGAAAAACTT ACTTTCTTCA CAAAGGAAAG ATTTAGAGAA GGGACAAAGA
    AAAGATTTAG GACGACATAG AGATTTGGTT GATGAAGACC TTTCACATTT CCAATCTGGA
    TCACCTCCTG CCCCAAATGG TTTTAAACAA CATGGGAATC CACATCTATA TCATAGTCAA
    GGAAAAGGAT CATATAAACA TGACCGAGTT GTACCTCAGA GTCGAGCTTC TGCACAAATA
    ATAAGTTCAA GTAAATCCCA GATTCTTGCT CCAGGAGAGA AAATAACTGG CAAAGTTAAG
    AGTGACAATG GCACTGGATA TGACACAGAC AGCAGCCAAG ATTCTAGGGA TAGAGGAAAC
    AGCTGTGATA GCAGCAGTAA AAGCCGGAAC CGAGGTTGGA AACCTATGAG AGAAACATTA
    AATGTTGATA GTATTTTTAG TGAAAGTGAA AAAAGACAGC ATAGTCCAAG ACATAAACCA
    AATATCAGTA ATAAGCCTAA ATCTAGCAAG GATCCGAGTT TTAGTAATTG GCCAAAAGAG
    AATCCAAAGC AAAAAGGTTT AATGACCATA TATGAAGATG AAATGAAGCA GGAAATAGGA
    AGCAGAAGTT CCCTTGAATC TAATGGAAAA GGAGCAGAGA AAAATAAAGG CCTTGTAGAG
    GGTAAAGTGC ATGGTGATAA TTGGCAGATG CAAAGGACTG AGTCTGGATA TGAAAGCAGT
    GATCACATCA GTAATGGTTC TACTAATTTG GACTCACCTG TTATCGATGG AAATGGTACA
    GTAATGGATA TCAGTGGTGT TAAAGAAACA GTATGCTTCA GTGACCAGAT TACGACAAGC
    AACCTAAATA AAGAACGTGG GGACTGTACC TCCCTTCAGA GCCAACATCA CTTAGAAGGC
    TTTAGAAAAG AACTCAGGAA TTTGGAAGCA GGCTATAAAT CTCATGAATT CCACCCAGAA
    TCACATTTAC AAATAAAAAA TCATTTGATA AAAAGATCAC ATGTACATGA AGACAATGGA
    AAGTTATTTC CTTCATCCAG TCTACAAATA CCCAAGGACC ATAATGCAAG AGAACATATC
    CACCAGTCAG ATGAACAGAA ACTTGAAAAA CCGAATGAAT GCAAATTTTC TGAGTGGCTT
    AATATAGAAA ATTCTGAGAG AACAGGTTTG CCTTTTCACG TTGATAACTC TGCTTCTGGG
    AAGAGAGTGA ACAGTAATGA ACCATCTTCA TTATGGTCTT CACACCTAAG AACTGTTGGG
    TTAAAGCCAG AAACTGCTCC TCTCATCCAG CAACAAAATA TCATGGATCA ATGTTACTTT
    GAGAACTCTC TATCCACAGA ATGTATAATT CGGTCAGCCA GCAGATCTGA TGGGTGTCAG
    ATGCCAAAAC TTTTTTGCCA GAATCTACCA CCCCCTTTGC CACCAAAGAA ATATGCTATA
    ACCAGTGTGC CACAGTCAGA GAAAAGCGAA TCTACACCTG ATGTCAAACT TACAGAGGTG
    TTTAAAGCTA CCTCTCATCT TCCGAAGCAC AGTTTAAGTA CAGCTTCAGA ACCAAGTTTA
    GAAGTGAGTA CACATATGAA TGATGAAAGA CATAAAGAAA CATTTCAAGT GAGAGAATGT
    TTTGGCAACA CACCAAACTG TCCATCCAGC TCCTCAACTA ACGATTTTCA GGCAAACTCA
    GGTGCCATTG ATGCATTTTG CCAACCAGAA CTAGACTCTA TTTCTACCTG TCCAAATGAG
    ACAGTTTCAT TAACTACCTA TTTTTCAGTT GATAGCTGCA TGACGGATAC ATATAGATTG
    AAATACCATC AGAGGCCCAA GCTCTCTTTT CCAGAGAGCA GTGGCTTTTG TAATAATTCA
    CTATCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005263133.1
    CDS1106..4174
    Translation

    Target ORF information:

    RefSeq Version XM_005263076.2
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005263076.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGCTAAG
    TTAAGTCACA TTGATCAAAG GGAAAAGATA AAAGACATTT CCAGAGAATG TGCTCTGAAA
    GCTATTGAAC AGAAAAACTT ACTTTCTTCA CAAAGGAAAG ATTTAGAGAA GGGACAAAGA
    AAAGATTTAG GACGACATAG AGATTTGGTT GATGAAGACC TTTCACATTT CCAATCTGGA
    TCACCTCCTG CCCCAAATGG TTTTAAACAA CATGGGAATC CACATCTATA TCATAGTCAA
    GGAAAAGGAT CATATAAACA TGACCGAGTT GTACCTCAGA GTCGAGCTTC TGCACAAATA
    ATAAGTTCAA GTAAATCCCA GATTCTTGCT CCAGGAGAGA AAATAACTGG CAAAGTTAAG
    AGTGACAATG GCACTGGATA TGACACAGAC AGCAGCCAAG ATTCTAGGGA TAGAGGAAAC
    AGCTGTGATA GCAGCAGTAA AAGCCGGAAC CGAGGTTGGA AACCTATGAG AGAAACATTA
    AATGTTGATA GTATTTTTAG TGAAAGTGAA AAAAGACAGC ATAGTCCAAG ACATAAACCA
    AATATCAGTA ATAAGCCTAA ATCTAGCAAG GATCCGAGTT TTAGTAATTG GCCAAAAGAG
    AATCCAAAGC AAAAAGGTTT AATGACCATA TATGAAGATG AAATGAAGCA GGAAATAGGA
    AGCAGAAGTT CCCTTGAATC TAATGGAAAA GGAGCAGAGA AAAATAAAGG CCTTGTAGAG
    GGTAAAGTGC ATGGTGATAA TTGGCAGATG CAAAGGACTG AGTCTGGATA TGAAAGCAGT
    GATCACATCA GTAATGGTTC TACTAATTTG GACTCACCTG TTATCGATGG AAATGGTACA
    GTAATGGATA TCAGTGGTGT TAAAGAAACA GTATGCTTCA GTGACCAGAT TACGACAAGC
    AACCTAAATA AAGAACGTGG GGACTGTACC TCCCTTCAGA GCCAACATCA CTTAGAAGGC
    TTTAGAAAAG AACTCAGGAA TTTGGAAGCA GGCTATAAAT CTCATGAATT CCACCCAGAA
    TCACATTTAC AAATAAAAAA TCATTTGATA AAAAGATCAC ATGTACATGA AGACAATGGA
    AAGTTATTTC CTTCATCCAG TCTACAAATA CCCAAGGACC ATAATGCAAG AGAACATATC
    CACCAGTCAG ATGAACAGAA ACTTGAAAAA CCGAATGAAT GCAAATTTTC TGAGTGGCTT
    AATATAGAAA ATTCTGAGAG AACAGGTTTG CCTTTTCACG TTGATAACTC TGCTTCTGGG
    AAGAGAGTGA ACAGTAATGA ACCATCTTCA TTATGGTCTT CACACCTAAG AACTGTTGGG
    TTAAAGCCAG AAACTGCTCC TCTCATCCAG CAACAAAATA TCATGGATCA ATGTTACTTT
    GAGAACTCTC TATCCACAGA ATGTATAATT CGGTCAGCCA GCAGATCTGA TGGGTGTCAG
    ATGCCAAAAC TTTTTTGCCA GAATCTACCA CCCCCTTTGC CACCAAAGAA ATATGCTATA
    ACCAGTGTGC CACAGTCAGA GAAAAGCGAA TCTACACCTG ATGTCAAACT TACAGAGGTG
    TTTAAAGCTA CCTCTCATCT TCCGAAGCAC AGTTTAAGTA CAGCTTCAGA ACCAAGTTTA
    GAAGTGAGTA CACATATGAA TGATGAAAGA CATAAAGAAA CATTTCAAGT GAGAGAATGT
    TTTGGCAACA CACCAAACTG TCCATCCAGC TCCTCAACTA ACGATTTTCA GGCAAACTCA
    GGTGCCATTG ATGCATTTTG CCAACCAGAA CTAGACTCTA TTTCTACCTG TCCAAATGAG
    ACAGTTTCAT TAACTACCTA TTTTTCAGTT GATAGCTGCA TGACGGATAC ATATAGATTG
    AAATACCATC AGAGGCCCAA GCTCTCTTTT CCAGAGAGCA GTGGCTTTTG TAATAATTCA
    CTATCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38975
    Clone ID Related Accession (Same CDS sequence) NM_001371396.1 , XM_005263076.2 , XM_017008316.1 , XM_017008316.2 , XM_005263076.3
    Accession Version XM_017008316.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3069bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGCTAAG
    TTAAGTCACA TTGATCAAAG GGAAAAGATA AAAGACATTT CCAGAGAATG TGCTCTGAAA
    GCTATTGAAC AGAAAAACTT ACTTTCTTCA CAAAGGAAAG ATTTAGAGAA GGGACAAAGA
    AAAGATTTAG GACGACATAG AGATTTGGTT GATGAAGACC TTTCACATTT CCAATCTGGA
    TCACCTCCTG CCCCAAATGG TTTTAAACAA CATGGGAATC CACATCTATA TCATAGTCAA
    GGAAAAGGAT CATATAAACA TGACCGAGTT GTACCTCAGA GTCGAGCTTC TGCACAAATA
    ATAAGTTCAA GTAAATCCCA GATTCTTGCT CCAGGAGAGA AAATAACTGG CAAAGTTAAG
    AGTGACAATG GCACTGGATA TGACACAGAC AGCAGCCAAG ATTCTAGGGA TAGAGGAAAC
    AGCTGTGATA GCAGCAGTAA AAGCCGGAAC CGAGGTTGGA AACCTATGAG AGAAACATTA
    AATGTTGATA GTATTTTTAG TGAAAGTGAA AAAAGACAGC ATAGTCCAAG ACATAAACCA
    AATATCAGTA ATAAGCCTAA ATCTAGCAAG GATCCGAGTT TTAGTAATTG GCCAAAAGAG
    AATCCAAAGC AAAAAGGTTT AATGACCATA TATGAAGATG AAATGAAGCA GGAAATAGGA
    AGCAGAAGTT CCCTTGAATC TAATGGAAAA GGAGCAGAGA AAAATAAAGG CCTTGTAGAG
    GGTAAAGTGC ATGGTGATAA TTGGCAGATG CAAAGGACTG AGTCTGGATA TGAAAGCAGT
    GATCACATCA GTAATGGTTC TACTAATTTG GACTCACCTG TTATCGATGG AAATGGTACA
    GTAATGGATA TCAGTGGTGT TAAAGAAACA GTATGCTTCA GTGACCAGAT TACGACAAGC
    AACCTAAATA AAGAACGTGG GGACTGTACC TCCCTTCAGA GCCAACATCA CTTAGAAGGC
    TTTAGAAAAG AACTCAGGAA TTTGGAAGCA GGCTATAAAT CTCATGAATT CCACCCAGAA
    TCACATTTAC AAATAAAAAA TCATTTGATA AAAAGATCAC ATGTACATGA AGACAATGGA
    AAGTTATTTC CTTCATCCAG TCTACAAATA CCCAAGGACC ATAATGCAAG AGAACATATC
    CACCAGTCAG ATGAACAGAA ACTTGAAAAA CCGAATGAAT GCAAATTTTC TGAGTGGCTT
    AATATAGAAA ATTCTGAGAG AACAGGTTTG CCTTTTCACG TTGATAACTC TGCTTCTGGG
    AAGAGAGTGA ACAGTAATGA ACCATCTTCA TTATGGTCTT CACACCTAAG AACTGTTGGG
    TTAAAGCCAG AAACTGCTCC TCTCATCCAG CAACAAAATA TCATGGATCA ATGTTACTTT
    GAGAACTCTC TATCCACAGA ATGTATAATT CGGTCAGCCA GCAGATCTGA TGGGTGTCAG
    ATGCCAAAAC TTTTTTGCCA GAATCTACCA CCCCCTTTGC CACCAAAGAA ATATGCTATA
    ACCAGTGTGC CACAGTCAGA GAAAAGCGAA TCTACACCTG ATGTCAAACT TACAGAGGTG
    TTTAAAGCTA CCTCTCATCT TCCGAAGCAC AGTTTAAGTA CAGCTTCAGA ACCAAGTTTA
    GAAGTGAGTA CACATATGAA TGATGAAAGA CATAAAGAAA CATTTCAAGT GAGAGAATGT
    TTTGGCAACA CACCAAACTG TCCATCCAGC TCCTCAACTA ACGATTTTCA GGCAAACTCA
    GGTGCCATTG ATGCATTTTG CCAACCAGAA CTAGACTCTA TTTCTACCTG TCCAAATGAG
    ACAGTTTCAT TAACTACCTA TTTTTCAGTT GATAGCTGCA TGACGGATAC ATATAGATTG
    AAATACCATC AGAGGCCCAA GCTCTCTTTT CCAGAGAGCA GTGGCTTTTG TAATAATTCA
    CTATCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863805.1
    CDS958..4026
    Translation

    Target ORF information:

    RefSeq Version XM_017008316.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008316.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGCTAAG
    TTAAGTCACA TTGATCAAAG GGAAAAGATA AAAGACATTT CCAGAGAATG TGCTCTGAAA
    GCTATTGAAC AGAAAAACTT ACTTTCTTCA CAAAGGAAAG ATTTAGAGAA GGGACAAAGA
    AAAGATTTAG GACGACATAG AGATTTGGTT GATGAAGACC TTTCACATTT CCAATCTGGA
    TCACCTCCTG CCCCAAATGG TTTTAAACAA CATGGGAATC CACATCTATA TCATAGTCAA
    GGAAAAGGAT CATATAAACA TGACCGAGTT GTACCTCAGA GTCGAGCTTC TGCACAAATA
    ATAAGTTCAA GTAAATCCCA GATTCTTGCT CCAGGAGAGA AAATAACTGG CAAAGTTAAG
    AGTGACAATG GCACTGGATA TGACACAGAC AGCAGCCAAG ATTCTAGGGA TAGAGGAAAC
    AGCTGTGATA GCAGCAGTAA AAGCCGGAAC CGAGGTTGGA AACCTATGAG AGAAACATTA
    AATGTTGATA GTATTTTTAG TGAAAGTGAA AAAAGACAGC ATAGTCCAAG ACATAAACCA
    AATATCAGTA ATAAGCCTAA ATCTAGCAAG GATCCGAGTT TTAGTAATTG GCCAAAAGAG
    AATCCAAAGC AAAAAGGTTT AATGACCATA TATGAAGATG AAATGAAGCA GGAAATAGGA
    AGCAGAAGTT CCCTTGAATC TAATGGAAAA GGAGCAGAGA AAAATAAAGG CCTTGTAGAG
    GGTAAAGTGC ATGGTGATAA TTGGCAGATG CAAAGGACTG AGTCTGGATA TGAAAGCAGT
    GATCACATCA GTAATGGTTC TACTAATTTG GACTCACCTG TTATCGATGG AAATGGTACA
    GTAATGGATA TCAGTGGTGT TAAAGAAACA GTATGCTTCA GTGACCAGAT TACGACAAGC
    AACCTAAATA AAGAACGTGG GGACTGTACC TCCCTTCAGA GCCAACATCA CTTAGAAGGC
    TTTAGAAAAG AACTCAGGAA TTTGGAAGCA GGCTATAAAT CTCATGAATT CCACCCAGAA
    TCACATTTAC AAATAAAAAA TCATTTGATA AAAAGATCAC ATGTACATGA AGACAATGGA
    AAGTTATTTC CTTCATCCAG TCTACAAATA CCCAAGGACC ATAATGCAAG AGAACATATC
    CACCAGTCAG ATGAACAGAA ACTTGAAAAA CCGAATGAAT GCAAATTTTC TGAGTGGCTT
    AATATAGAAA ATTCTGAGAG AACAGGTTTG CCTTTTCACG TTGATAACTC TGCTTCTGGG
    AAGAGAGTGA ACAGTAATGA ACCATCTTCA TTATGGTCTT CACACCTAAG AACTGTTGGG
    TTAAAGCCAG AAACTGCTCC TCTCATCCAG CAACAAAATA TCATGGATCA ATGTTACTTT
    GAGAACTCTC TATCCACAGA ATGTATAATT CGGTCAGCCA GCAGATCTGA TGGGTGTCAG
    ATGCCAAAAC TTTTTTGCCA GAATCTACCA CCCCCTTTGC CACCAAAGAA ATATGCTATA
    ACCAGTGTGC CACAGTCAGA GAAAAGCGAA TCTACACCTG ATGTCAAACT TACAGAGGTG
    TTTAAAGCTA CCTCTCATCT TCCGAAGCAC AGTTTAAGTA CAGCTTCAGA ACCAAGTTTA
    GAAGTGAGTA CACATATGAA TGATGAAAGA CATAAAGAAA CATTTCAAGT GAGAGAATGT
    TTTGGCAACA CACCAAACTG TCCATCCAGC TCCTCAACTA ACGATTTTCA GGCAAACTCA
    GGTGCCATTG ATGCATTTTG CCAACCAGAA CTAGACTCTA TTTCTACCTG TCCAAATGAG
    ACAGTTTCAT TAACTACCTA TTTTTCAGTT GATAGCTGCA TGACGGATAC ATATAGATTG
    AAATACCATC AGAGGCCCAA GCTCTCTTTT CCAGAGAGCA GTGGCTTTTG TAATAATTCA
    CTATCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38975
    Clone ID Related Accession (Same CDS sequence) NM_001371396.1 , XM_005263076.2 , XM_017008316.1 , XM_017008316.2 , XM_005263076.3
    Accession Version XM_017008316.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3069bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-12
    Organism Homo sapiens(human)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017008316.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGCTAAG
    TTAAGTCACA TTGATCAAAG GGAAAAGATA AAAGACATTT CCAGAGAATG TGCTCTGAAA
    GCTATTGAAC AGAAAAACTT ACTTTCTTCA CAAAGGAAAG ATTTAGAGAA GGGACAAAGA
    AAAGATTTAG GACGACATAG AGATTTGGTT GATGAAGACC TTTCACATTT CCAATCTGGA
    TCACCTCCTG CCCCAAATGG TTTTAAACAA CATGGGAATC CACATCTATA TCATAGTCAA
    GGAAAAGGAT CATATAAACA TGACCGAGTT GTACCTCAGA GTCGAGCTTC TGCACAAATA
    ATAAGTTCAA GTAAATCCCA GATTCTTGCT CCAGGAGAGA AAATAACTGG CAAAGTTAAG
    AGTGACAATG GCACTGGATA TGACACAGAC AGCAGCCAAG ATTCTAGGGA TAGAGGAAAC
    AGCTGTGATA GCAGCAGTAA AAGCCGGAAC CGAGGTTGGA AACCTATGAG AGAAACATTA
    AATGTTGATA GTATTTTTAG TGAAAGTGAA AAAAGACAGC ATAGTCCAAG ACATAAACCA
    AATATCAGTA ATAAGCCTAA ATCTAGCAAG GATCCGAGTT TTAGTAATTG GCCAAAAGAG
    AATCCAAAGC AAAAAGGTTT AATGACCATA TATGAAGATG AAATGAAGCA GGAAATAGGA
    AGCAGAAGTT CCCTTGAATC TAATGGAAAA GGAGCAGAGA AAAATAAAGG CCTTGTAGAG
    GGTAAAGTGC ATGGTGATAA TTGGCAGATG CAAAGGACTG AGTCTGGATA TGAAAGCAGT
    GATCACATCA GTAATGGTTC TACTAATTTG GACTCACCTG TTATCGATGG AAATGGTACA
    GTAATGGATA TCAGTGGTGT TAAAGAAACA GTATGCTTCA GTGACCAGAT TACGACAAGC
    AACCTAAATA AAGAACGTGG GGACTGTACC TCCCTTCAGA GCCAACATCA CTTAGAAGGC
    TTTAGAAAAG AACTCAGGAA TTTGGAAGCA GGCTATAAAT CTCATGAATT CCACCCAGAA
    TCACATTTAC AAATAAAAAA TCATTTGATA AAAAGATCAC ATGTACATGA AGACAATGGA
    AAGTTATTTC CTTCATCCAG TCTACAAATA CCCAAGGACC ATAATGCAAG AGAACATATC
    CACCAGTCAG ATGAACAGAA ACTTGAAAAA CCGAATGAAT GCAAATTTTC TGAGTGGCTT
    AATATAGAAA ATTCTGAGAG AACAGGTTTG CCTTTTCACG TTGATAACTC TGCTTCTGGG
    AAGAGAGTGA ACAGTAATGA ACCATCTTCA TTATGGTCTT CACACCTAAG AACTGTTGGG
    TTAAAGCCAG AAACTGCTCC TCTCATCCAG CAACAAAATA TCATGGATCA ATGTTACTTT
    GAGAACTCTC TATCCACAGA ATGTATAATT CGGTCAGCCA GCAGATCTGA TGGGTGTCAG
    ATGCCAAAAC TTTTTTGCCA GAATCTACCA CCCCCTTTGC CACCAAAGAA ATATGCTATA
    ACCAGTGTGC CACAGTCAGA GAAAAGCGAA TCTACACCTG ATGTCAAACT TACAGAGGTG
    TTTAAAGCTA CCTCTCATCT TCCGAAGCAC AGTTTAAGTA CAGCTTCAGA ACCAAGTTTA
    GAAGTGAGTA CACATATGAA TGATGAAAGA CATAAAGAAA CATTTCAAGT GAGAGAATGT
    TTTGGCAACA CACCAAACTG TCCATCCAGC TCCTCAACTA ACGATTTTCA GGCAAACTCA
    GGTGCCATTG ATGCATTTTG CCAACCAGAA CTAGACTCTA TTTCTACCTG TCCAAATGAG
    ACAGTTTCAT TAACTACCTA TTTTTCAGTT GATAGCTGCA TGACGGATAC ATATAGATTG
    AAATACCATC AGAGGCCCAA GCTCTCTTTT CCAGAGAGCA GTGGCTTTTG TAATAATTCA
    CTATCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863805.1
    CDS899..3967
    Translation

    Target ORF information:

    RefSeq Version XM_017008316.2
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008316.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGCTAAG
    TTAAGTCACA TTGATCAAAG GGAAAAGATA AAAGACATTT CCAGAGAATG TGCTCTGAAA
    GCTATTGAAC AGAAAAACTT ACTTTCTTCA CAAAGGAAAG ATTTAGAGAA GGGACAAAGA
    AAAGATTTAG GACGACATAG AGATTTGGTT GATGAAGACC TTTCACATTT CCAATCTGGA
    TCACCTCCTG CCCCAAATGG TTTTAAACAA CATGGGAATC CACATCTATA TCATAGTCAA
    GGAAAAGGAT CATATAAACA TGACCGAGTT GTACCTCAGA GTCGAGCTTC TGCACAAATA
    ATAAGTTCAA GTAAATCCCA GATTCTTGCT CCAGGAGAGA AAATAACTGG CAAAGTTAAG
    AGTGACAATG GCACTGGATA TGACACAGAC AGCAGCCAAG ATTCTAGGGA TAGAGGAAAC
    AGCTGTGATA GCAGCAGTAA AAGCCGGAAC CGAGGTTGGA AACCTATGAG AGAAACATTA
    AATGTTGATA GTATTTTTAG TGAAAGTGAA AAAAGACAGC ATAGTCCAAG ACATAAACCA
    AATATCAGTA ATAAGCCTAA ATCTAGCAAG GATCCGAGTT TTAGTAATTG GCCAAAAGAG
    AATCCAAAGC AAAAAGGTTT AATGACCATA TATGAAGATG AAATGAAGCA GGAAATAGGA
    AGCAGAAGTT CCCTTGAATC TAATGGAAAA GGAGCAGAGA AAAATAAAGG CCTTGTAGAG
    GGTAAAGTGC ATGGTGATAA TTGGCAGATG CAAAGGACTG AGTCTGGATA TGAAAGCAGT
    GATCACATCA GTAATGGTTC TACTAATTTG GACTCACCTG TTATCGATGG AAATGGTACA
    GTAATGGATA TCAGTGGTGT TAAAGAAACA GTATGCTTCA GTGACCAGAT TACGACAAGC
    AACCTAAATA AAGAACGTGG GGACTGTACC TCCCTTCAGA GCCAACATCA CTTAGAAGGC
    TTTAGAAAAG AACTCAGGAA TTTGGAAGCA GGCTATAAAT CTCATGAATT CCACCCAGAA
    TCACATTTAC AAATAAAAAA TCATTTGATA AAAAGATCAC ATGTACATGA AGACAATGGA
    AAGTTATTTC CTTCATCCAG TCTACAAATA CCCAAGGACC ATAATGCAAG AGAACATATC
    CACCAGTCAG ATGAACAGAA ACTTGAAAAA CCGAATGAAT GCAAATTTTC TGAGTGGCTT
    AATATAGAAA ATTCTGAGAG AACAGGTTTG CCTTTTCACG TTGATAACTC TGCTTCTGGG
    AAGAGAGTGA ACAGTAATGA ACCATCTTCA TTATGGTCTT CACACCTAAG AACTGTTGGG
    TTAAAGCCAG AAACTGCTCC TCTCATCCAG CAACAAAATA TCATGGATCA ATGTTACTTT
    GAGAACTCTC TATCCACAGA ATGTATAATT CGGTCAGCCA GCAGATCTGA TGGGTGTCAG
    ATGCCAAAAC TTTTTTGCCA GAATCTACCA CCCCCTTTGC CACCAAAGAA ATATGCTATA
    ACCAGTGTGC CACAGTCAGA GAAAAGCGAA TCTACACCTG ATGTCAAACT TACAGAGGTG
    TTTAAAGCTA CCTCTCATCT TCCGAAGCAC AGTTTAAGTA CAGCTTCAGA ACCAAGTTTA
    GAAGTGAGTA CACATATGAA TGATGAAAGA CATAAAGAAA CATTTCAAGT GAGAGAATGT
    TTTGGCAACA CACCAAACTG TCCATCCAGC TCCTCAACTA ACGATTTTCA GGCAAACTCA
    GGTGCCATTG ATGCATTTTG CCAACCAGAA CTAGACTCTA TTTCTACCTG TCCAAATGAG
    ACAGTTTCAT TAACTACCTA TTTTTCAGTT GATAGCTGCA TGACGGATAC ATATAGATTG
    AAATACCATC AGAGGCCCAA GCTCTCTTTT CCAGAGAGCA GTGGCTTTTG TAATAATTCA
    CTATCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38975
    Clone ID Related Accession (Same CDS sequence) NM_001371396.1 , XM_005263076.2 , XM_017008316.1 , XM_017008316.2 , XM_005263076.3
    Accession Version XM_005263076.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3069bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005263076.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGCTAAG
    TTAAGTCACA TTGATCAAAG GGAAAAGATA AAAGACATTT CCAGAGAATG TGCTCTGAAA
    GCTATTGAAC AGAAAAACTT ACTTTCTTCA CAAAGGAAAG ATTTAGAGAA GGGACAAAGA
    AAAGATTTAG GACGACATAG AGATTTGGTT GATGAAGACC TTTCACATTT CCAATCTGGA
    TCACCTCCTG CCCCAAATGG TTTTAAACAA CATGGGAATC CACATCTATA TCATAGTCAA
    GGAAAAGGAT CATATAAACA TGACCGAGTT GTACCTCAGA GTCGAGCTTC TGCACAAATA
    ATAAGTTCAA GTAAATCCCA GATTCTTGCT CCAGGAGAGA AAATAACTGG CAAAGTTAAG
    AGTGACAATG GCACTGGATA TGACACAGAC AGCAGCCAAG ATTCTAGGGA TAGAGGAAAC
    AGCTGTGATA GCAGCAGTAA AAGCCGGAAC CGAGGTTGGA AACCTATGAG AGAAACATTA
    AATGTTGATA GTATTTTTAG TGAAAGTGAA AAAAGACAGC ATAGTCCAAG ACATAAACCA
    AATATCAGTA ATAAGCCTAA ATCTAGCAAG GATCCGAGTT TTAGTAATTG GCCAAAAGAG
    AATCCAAAGC AAAAAGGTTT AATGACCATA TATGAAGATG AAATGAAGCA GGAAATAGGA
    AGCAGAAGTT CCCTTGAATC TAATGGAAAA GGAGCAGAGA AAAATAAAGG CCTTGTAGAG
    GGTAAAGTGC ATGGTGATAA TTGGCAGATG CAAAGGACTG AGTCTGGATA TGAAAGCAGT
    GATCACATCA GTAATGGTTC TACTAATTTG GACTCACCTG TTATCGATGG AAATGGTACA
    GTAATGGATA TCAGTGGTGT TAAAGAAACA GTATGCTTCA GTGACCAGAT TACGACAAGC
    AACCTAAATA AAGAACGTGG GGACTGTACC TCCCTTCAGA GCCAACATCA CTTAGAAGGC
    TTTAGAAAAG AACTCAGGAA TTTGGAAGCA GGCTATAAAT CTCATGAATT CCACCCAGAA
    TCACATTTAC AAATAAAAAA TCATTTGATA AAAAGATCAC ATGTACATGA AGACAATGGA
    AAGTTATTTC CTTCATCCAG TCTACAAATA CCCAAGGACC ATAATGCAAG AGAACATATC
    CACCAGTCAG ATGAACAGAA ACTTGAAAAA CCGAATGAAT GCAAATTTTC TGAGTGGCTT
    AATATAGAAA ATTCTGAGAG AACAGGTTTG CCTTTTCACG TTGATAACTC TGCTTCTGGG
    AAGAGAGTGA ACAGTAATGA ACCATCTTCA TTATGGTCTT CACACCTAAG AACTGTTGGG
    TTAAAGCCAG AAACTGCTCC TCTCATCCAG CAACAAAATA TCATGGATCA ATGTTACTTT
    GAGAACTCTC TATCCACAGA ATGTATAATT CGGTCAGCCA GCAGATCTGA TGGGTGTCAG
    ATGCCAAAAC TTTTTTGCCA GAATCTACCA CCCCCTTTGC CACCAAAGAA ATATGCTATA
    ACCAGTGTGC CACAGTCAGA GAAAAGCGAA TCTACACCTG ATGTCAAACT TACAGAGGTG
    TTTAAAGCTA CCTCTCATCT TCCGAAGCAC AGTTTAAGTA CAGCTTCAGA ACCAAGTTTA
    GAAGTGAGTA CACATATGAA TGATGAAAGA CATAAAGAAA CATTTCAAGT GAGAGAATGT
    TTTGGCAACA CACCAAACTG TCCATCCAGC TCCTCAACTA ACGATTTTCA GGCAAACTCA
    GGTGCCATTG ATGCATTTTG CCAACCAGAA CTAGACTCTA TTTCTACCTG TCCAAATGAG
    ACAGTTTCAT TAACTACCTA TTTTTCAGTT GATAGCTGCA TGACGGATAC ATATAGATTG
    AAATACCATC AGAGGCCCAA GCTCTCTTTT CCAGAGAGCA GTGGCTTTTG TAATAATTCA
    CTATCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005263133.1
    CDS1067..4135
    Translation

    Target ORF information:

    RefSeq Version XM_005263076.3
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005263076.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
    GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC
    TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG
    CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG
    ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT
    GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT
    GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT
    GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT
    GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT
    GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT
    GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC
    TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT
    TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA
    GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT
    GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA
    CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA
    AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC
    TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT
    GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGCTAAG
    TTAAGTCACA TTGATCAAAG GGAAAAGATA AAAGACATTT CCAGAGAATG TGCTCTGAAA
    GCTATTGAAC AGAAAAACTT ACTTTCTTCA CAAAGGAAAG ATTTAGAGAA GGGACAAAGA
    AAAGATTTAG GACGACATAG AGATTTGGTT GATGAAGACC TTTCACATTT CCAATCTGGA
    TCACCTCCTG CCCCAAATGG TTTTAAACAA CATGGGAATC CACATCTATA TCATAGTCAA
    GGAAAAGGAT CATATAAACA TGACCGAGTT GTACCTCAGA GTCGAGCTTC TGCACAAATA
    ATAAGTTCAA GTAAATCCCA GATTCTTGCT CCAGGAGAGA AAATAACTGG CAAAGTTAAG
    AGTGACAATG GCACTGGATA TGACACAGAC AGCAGCCAAG ATTCTAGGGA TAGAGGAAAC
    AGCTGTGATA GCAGCAGTAA AAGCCGGAAC CGAGGTTGGA AACCTATGAG AGAAACATTA
    AATGTTGATA GTATTTTTAG TGAAAGTGAA AAAAGACAGC ATAGTCCAAG ACATAAACCA
    AATATCAGTA ATAAGCCTAA ATCTAGCAAG GATCCGAGTT TTAGTAATTG GCCAAAAGAG
    AATCCAAAGC AAAAAGGTTT AATGACCATA TATGAAGATG AAATGAAGCA GGAAATAGGA
    AGCAGAAGTT CCCTTGAATC TAATGGAAAA GGAGCAGAGA AAAATAAAGG CCTTGTAGAG
    GGTAAAGTGC ATGGTGATAA TTGGCAGATG CAAAGGACTG AGTCTGGATA TGAAAGCAGT
    GATCACATCA GTAATGGTTC TACTAATTTG GACTCACCTG TTATCGATGG AAATGGTACA
    GTAATGGATA TCAGTGGTGT TAAAGAAACA GTATGCTTCA GTGACCAGAT TACGACAAGC
    AACCTAAATA AAGAACGTGG GGACTGTACC TCCCTTCAGA GCCAACATCA CTTAGAAGGC
    TTTAGAAAAG AACTCAGGAA TTTGGAAGCA GGCTATAAAT CTCATGAATT CCACCCAGAA
    TCACATTTAC AAATAAAAAA TCATTTGATA AAAAGATCAC ATGTACATGA AGACAATGGA
    AAGTTATTTC CTTCATCCAG TCTACAAATA CCCAAGGACC ATAATGCAAG AGAACATATC
    CACCAGTCAG ATGAACAGAA ACTTGAAAAA CCGAATGAAT GCAAATTTTC TGAGTGGCTT
    AATATAGAAA ATTCTGAGAG AACAGGTTTG CCTTTTCACG TTGATAACTC TGCTTCTGGG
    AAGAGAGTGA ACAGTAATGA ACCATCTTCA TTATGGTCTT CACACCTAAG AACTGTTGGG
    TTAAAGCCAG AAACTGCTCC TCTCATCCAG CAACAAAATA TCATGGATCA ATGTTACTTT
    GAGAACTCTC TATCCACAGA ATGTATAATT CGGTCAGCCA GCAGATCTGA TGGGTGTCAG
    ATGCCAAAAC TTTTTTGCCA GAATCTACCA CCCCCTTTGC CACCAAAGAA ATATGCTATA
    ACCAGTGTGC CACAGTCAGA GAAAAGCGAA TCTACACCTG ATGTCAAACT TACAGAGGTG
    TTTAAAGCTA CCTCTCATCT TCCGAAGCAC AGTTTAAGTA CAGCTTCAGA ACCAAGTTTA
    GAAGTGAGTA CACATATGAA TGATGAAAGA CATAAAGAAA CATTTCAAGT GAGAGAATGT
    TTTGGCAACA CACCAAACTG TCCATCCAGC TCCTCAACTA ACGATTTTCA GGCAAACTCA
    GGTGCCATTG ATGCATTTTG CCAACCAGAA CTAGACTCTA TTTCTACCTG TCCAAATGAG
    ACAGTTTCAT TAACTACCTA TTTTTCAGTT GATAGCTGCA TGACGGATAC ATATAGATTG
    AAATACCATC AGAGGCCCAA GCTCTCTTTT CCAGAGAGCA GTGGCTTTTG TAATAATTCA
    CTATCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.