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POU3F2 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol POU3F2
Entrez Gene ID 5454
Full Name POU class 3 homeobox 2
Synonyms BRN2,N-Oct3,OCT7,OTF-7,OTF7,POUF3,brn-2,oct-7
General protein information
Preferred Names

POU class 3 homeobox 2

Names

POU domain, class 3, transcription factor 2
brain-2
brain-specific homeobox/POU domain protein 2
nervous system-specific octamer-binding transcription factor N-Oct-3
octamer-binding protein 7
octamer-binding transcription factor 7

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

6

6q16.1

Summary This gene encodes a member of the POU-III class of neural transcription factors. The encoded protein is involved in neuronal differentiation and enhances the activation of corticotropin-releasing hormone regulated genes. Overexpression of this protein is associated with an increase in the proliferation of melanoma cells. [provided by RefSeq, Mar 2012].
Disorder MIM:

600494


mRNA and Protein(s)

mRNA Protein Name
NM_005604.3 NP_005595.2 POU domain, class 3, transcription factor 2



Pan troglodytes (chimpanzee) POU3F2 XP_003950948.1
Bos taurus (cattle) POU3F2 NP_001073817.1
Macaca mulatta (rhesus monkey) LOC694524 XP_001083202.1
Xenopus tropicalis (tropical clawed frog) LOC100486226 NP_001263306.1
Rattus norvegicus (Norway rat) Pou3f2 NP_742082.1
Danio rerio (zebrafish) pou3f2b NP_571235.1
Homo sapiens (human) POU3F2 NP_005595.2
Canis lupus familiaris (dog) LOC481931 XP_539052.3
Mus musculus (house mouse) Pou3f2 NP_032925.1


Related articles in PubMed

NFIB Mediates BRN2 Driven Melanoma Cell Migration and Invasion Through Regulation of EZH2 and MITF.
Fane ME, Chhabra Y, Hollingsworth DE, Simmons JL, Spoerri L, Oh TG, Chauhan J, Chin T, Harris L, Harvey TJ, Muscat GE, Goding CR, Sturm RA, Haass NK, Boyle GM, Piper M, Smith AG
EBioMedicine1663-75(2017 Feb)

Capsaicin Inhibited Aggressive Phenotypes through Downregulation of Tumor-Associated NADH Oxidase (tNOX) by POU Domain Transcription Factor POU3F2.
Chen HY, Lee YH, Chen HY, Yeh CA, Chueh PJ, Lin YM
Molecules (Basel, Switzerland)21(6)(2016 Jun)

Small 6q16.1 Deletions Encompassing POU3F2 Cause Susceptibility to Obesity and Variable Developmental Delay with Intellectual Disability.
Kasher PR, Schertz KE, Thomas M, Jackson A, Annunziata S, Ballesta-Martinez MJ, Campeau PM, Clayton PE, Eaton JL, Granata T, Guillén-Navarro E, Hernando C, Laverriere CE, Liedén A, Villa-Marcos O, McEntagart M, Nordgren A, Pantaleoni C, Pebrel-Richard C, Sarret C, Sciacca FL, Wright R, Kerr B, Glasgow E, Banka S
American journal of human genetics98(2)363-72(2016 Feb)

Common genetic variants associated with cognitive performance identified using the proxy-phenotype method.
Rietveld CA, Esko T, Davies G, Pers TH, Turley P, Benyamin B, Chabris CF, Emilsson V, Johnson AD, Lee JJ, de Leeuw C, Marioni RE, Medland SE, Miller MB, Rostapshova O, van der Lee SJ, Vinkhuyzen AA, Amin N, Conley D, Derringer J, van Duijn CM, Fehrmann R, Franke L, Glaeser EL, Hansell NK, Hayward C, Iacono WG, Ibrahim-Verbaas C, Jaddoe V, Karjalainen J, Laibson D, Lichtenstein P, Liewald DC, Magnusson PK, Martin NG, McGue M, McMahon G, Pedersen NL, Pinker S, Porteous DJ, Posthuma D, Rivadeneira F, Smith BH, Starr JM, Tiemeier H, Timpson NJ, Trzaskowski M, Uitterlinden AG, Verhulst FC, Ward ME, Wright MJ, Davey Smith G, Deary IJ, Johannesson M, Plomin R, Visscher PM, Benjamin DJ, Cesarini D, Koellinger PD
Proceedings of the National Academy of Sciences of the United States of America111(38)13790-4(2014 Sep)

cDNA cloning of human N-Oct3, a nervous-system specific POU domain transcription factor binding to the octamer DNA motif.
Schreiber E, Tobler A, Malipiero U, Schaffner W, Fontana A
Nucleic acids research21(2)253-8(1993 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that NFIB protein increases EZH2 protein expression downstream of BRN2 protein, which further decreases MITF protein levels.
Title: NFIB Mediates BRN2 Driven Melanoma Cell Migration and Invasion Through Regulation of EZH2 and MITF.

These findings not only shed light on the molecular mechanism of the anticancer properties of capsaicin, but also the transcription regulation of tNOX expression that may potentially explain how POU3F2 is associated with tumorigenesis.
Title: Capsaicin Inhibited Aggressive Phenotypes through Downregulation of Tumor-Associated NADH Oxidase (tNOX) by POU Domain Transcription Factor POU3F2.

We show that POU3F2 lies downstreamof SIM1 and controls oxytocin expression in the hypothalamic neuroendocrine preoptic area. We show that this finding is consistent with the expression patterns of POU3F2 and related genes in the human brain.
Title: Small 6q16.1 Deletions Encompassing POU3F2 Cause Susceptibility to Obesity and Variable Developmental Delay with Intellectual Disability.

TTF1 expression in small-cell lung cancer is a cell lineage-specific phenomenon that involves the developing neural cell-specific homeoprotein BRN2.
Title: Neural lineage-specific homeoprotein BRN2 is directly involved in TTF1 expression in small-cell lung cancer.

BRN2 is a higher level regulator than ASCL1 and ND1 and BRN2 might be involved in aggressiveness of small cell lung cancer.
Title: POU domain transcription factor BRN2 is crucial for expression of ASCL1, ND1 and neuroendocrine marker molecules and cell growth in small cell lung cancer.

The following POU3F2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POU3F2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu10724 NM_005604.3
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Homo sapiens POU class 3 homeobox 2 (POU3F2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $223.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu10724
Clone ID Related Accession (Same CDS sequence) NM_005604.3
Accession Version NM_005604.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1332bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product POU domain, class 3, transcription factor 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA491157.1, BC051699.1 and AL022395.2. On Mar 14, 2012 this sequence version replaced NM_005604.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: BC051699.1 [ECO:0000345] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCGACCG CAGCGTCTAA CCACTACAGC CTGCTCACCT CCAGCGCCTC CATCGTGCAC 
GCCGAGCCGC CCGGCGGCAT GCAGCAGGGC GCGGGGGGCT ACCGCGAAGC GCAGAGCCTG
GTGCAGGGCG ACTACGGCGC TCTGCAGAGC AACGGACACC CGCTCAGCCA CGCTCACCAG
TGGATCACCG CGCTGTCCCA CGGCGGCGGC GGCGGGGGCG GTGGCGGCGG CGGGGGGGGC
GGGGGCGGCG GCGGGGGCGG CGGCGACGGC TCCCCGTGGT CCACCAGCCC CCTGGGCCAG
CCGGACATCA AGCCCTCGGT GGTGGTGCAG CAGGGCGGCC GCGGAGACGA GCTGCACGGG
CCAGGCGCCC TGCAGCAGCA GCATCAGCAG CAGCAACAGC AACAGCAGCA GCAACAGCAG
CAACAGCAGC AGCAGCAGCA GCAACAGCGG CCGCCGCATC TGGTGCACCA CGCCGCTAAC
CACCACCCGG GACCCGGGGC ATGGCGGAGC GCGGCGGCTG CAGCGCACCT CCCACCCTCC
ATGGGAGCGT CCAACGGCGG CTTGCTCTAC TCGCAGCCCA GCTTCACGGT GAACGGCATG
CTGGGCGCCG GCGGGCAGCC GGCCGGTCTG CACCACCACG GCCTGCGGGA CGCGCACGAC
GAGCCACACC ATGCCGACCA CCACCCGCAC CCGCACTCGC ACCCACACCA GCAGCCGCCG
CCCCCGCCGC CCCCGCAGGG TCCGCCTGGC CACCCAGGCG CGCACCACGA CCCGCACTCG
GACGAGGACA CGCCGACCTC GGACGACCTG GAGCAGTTCG CCAAGCAGTT CAAGCAGCGG
CGGATCAAAC TGGGATTTAC CCAAGCGGAC GTGGGGCTGG CTCTGGGCAC CCTGTATGGC
AACGTGTTCT CGCAGACCAC CATCTGCAGG TTTGAGGCCC TGCAGCTGAG CTTCAAGAAC
ATGTGCAAGC TGAAGCCTTT GTTGAACAAG TGGTTGGAGG AGGCGGACTC GTCCTCGGGC
AGCCCCACGA GCATAGACAA GATCGCAGCG CAAGGGCGCA AGCGGAAAAA GCGGACCTCC
ATCGAGGTGA GCGTCAAGGG GGCTCTGGAG AGCCATTTCC TCAAATGCCC CAAGCCCTCG
GCCCAGGAGA TCACCTCCCT CGCGGACAGC TTACAGCTGG AGAAGGAGGT GGTGAGAGTT
TGGTTTTGTA ACAGGAGACA GAAAGAGAAA AGGATGACCC CTCCCGGAGG GACTCTGCCG
GGCGCCGAGG ATGTGTACGG GGGGAGTAGG GACACTCCAC CACACCACGG GGTGCAGACG
CCCGTCCAGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005595.2
CDS171..1502
Misc Feature(1)6..8
Misc Feature(2)954..1178
Misc Feature(3)1191..1193
Misc Feature(4)order(1233..1247,1251..1253,1302..1304,1320..1322,1359..1361,1365..1370,1377..1382,1386..1394,1398..1403)
Misc Feature(5)1239..1400
Misc Feature(6)order(1239..1241,1248..1250,1368..1370,1377..1382,1389..1391)
Exon (1)1..4087
Translation

Target ORF information:

RefSeq Version NM_005604.3
Organism Homo sapiens(Human)
Definition Homo sapiens POU class 3 homeobox 2 (POU3F2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005604.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCGACCG CAGCGTCTAA CCACTACAGC CTGCTCACCT CCAGCGCCTC CATCGTGCAC 
GCCGAGCCGC CCGGCGGCAT GCAGCAGGGC GCGGGGGGCT ACCGCGAAGC GCAGAGCCTG
GTGCAGGGCG ACTACGGCGC TCTGCAGAGC AACGGACACC CGCTCAGCCA CGCTCACCAG
TGGATCACCG CGCTGTCCCA CGGCGGCGGC GGCGGGGGCG GTGGCGGCGG CGGGGGGGGC
GGGGGCGGCG GCGGGGGCGG CGGCGACGGC TCCCCGTGGT CCACCAGCCC CCTGGGCCAG
CCGGACATCA AGCCCTCGGT GGTGGTGCAG CAGGGCGGCC GCGGAGACGA GCTGCACGGG
CCAGGCGCCC TGCAGCAGCA GCATCAGCAG CAGCAACAGC AACAGCAGCA GCAACAGCAG
CAACAGCAGC AGCAGCAGCA GCAACAGCGG CCGCCGCATC TGGTGCACCA CGCCGCTAAC
CACCACCCGG GACCCGGGGC ATGGCGGAGC GCGGCGGCTG CAGCGCACCT CCCACCCTCC
ATGGGAGCGT CCAACGGCGG CTTGCTCTAC TCGCAGCCCA GCTTCACGGT GAACGGCATG
CTGGGCGCCG GCGGGCAGCC GGCCGGTCTG CACCACCACG GCCTGCGGGA CGCGCACGAC
GAGCCACACC ATGCCGACCA CCACCCGCAC CCGCACTCGC ACCCACACCA GCAGCCGCCG
CCCCCGCCGC CCCCGCAGGG TCCGCCTGGC CACCCAGGCG CGCACCACGA CCCGCACTCG
GACGAGGACA CGCCGACCTC GGACGACCTG GAGCAGTTCG CCAAGCAGTT CAAGCAGCGG
CGGATCAAAC TGGGATTTAC CCAAGCGGAC GTGGGGCTGG CTCTGGGCAC CCTGTATGGC
AACGTGTTCT CGCAGACCAC CATCTGCAGG TTTGAGGCCC TGCAGCTGAG CTTCAAGAAC
ATGTGCAAGC TGAAGCCTTT GTTGAACAAG TGGTTGGAGG AGGCGGACTC GTCCTCGGGC
AGCCCCACGA GCATAGACAA GATCGCAGCG CAAGGGCGCA AGCGGAAAAA GCGGACCTCC
ATCGAGGTGA GCGTCAAGGG GGCTCTGGAG AGCCATTTCC TCAAATGCCC CAAGCCCTCG
GCCCAGGAGA TCACCTCCCT CGCGGACAGC TTACAGCTGG AGAAGGAGGT GGTGAGAGTT
TGGTTTTGTA ACAGGAGACA GAAAGAGAAA AGGATGACCC CTCCCGGAGG GACTCTGCCG
GGCGCCGAGG ATGTGTACGG GGGGAGTAGG GACACTCCAC CACACCACGG GGTGCAGACG
CCCGTCCAGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

NFIB Mediates BRN2 Driven Melanoma Cell Migration and Invasion Through Regulation of EZH2 and MITF.
EBioMedicine1663-75(2017 Feb)
Fane ME,Chhabra Y,Hollingsworth DE,Simmons JL,Spoerri L,Oh TG,Chauhan J,Chin T,Harris L,Harvey TJ,Muscat GE,Goding CR,Sturm RA,Haass NK,Boyle GM,Piper M,Smith AG


book

Capsaicin Inhibited Aggressive Phenotypes through Downregulation of Tumor-Associated NADH Oxidase (tNOX) by POU Domain Transcription Factor POU3F2.
Molecules (Basel, Switzerland)21(6)(2016 Jun)
Chen HY,Lee YH,Chen HY,Yeh CA,Chueh PJ,Lin YM


book

Small 6q16.1 Deletions Encompassing POU3F2 Cause Susceptibility to Obesity and Variable Developmental Delay with Intellectual Disability.
American journal of human genetics98(2)363-72(2016 Feb)
Kasher PR,Schertz KE,Thomas M,Jackson A,Annunziata S,Ballesta-Martinez MJ,Campeau PM,Clayton PE,Eaton JL,Granata T,Guillén-Navarro E,Hernando C,Laverriere CE,Liedén A,Villa-Marcos O,McEntagart M,Nordgren A,Pantaleoni C,Pebrel-Richard C,Sarret C,Sciacca FL,Wright R,Kerr B,Glasgow E,Banka S


book

Common genetic variants associated with cognitive performance identified using the proxy-phenotype method.
Proceedings of the National Academy of Sciences of the United States of America111(38)13790-4(2014 Sep)
Rietveld CA,Esko T,Davies G,Pers TH,Turley P,Benyamin B,Chabris CF,Emilsson V,Johnson AD,Lee JJ,de Leeuw C,Marioni RE,Medland SE,Miller MB,Rostapshova O,van der Lee SJ,Vinkhuyzen AA,Amin N,Conley D,Derringer J,van Duijn CM,Fehrmann R,Franke L,Glaeser EL,Hansell NK,Hayward C,Iacono WG,Ibrahim-Verbaas C,Jaddoe V,Karjalainen J,Laibson D,Lichtenstein P,Liewald DC,Magnusson PK,Martin NG,McGue M,McMahon G,Pedersen NL,Pinker S,Porteous DJ,Posthuma D,Rivadeneira F,Smith BH,Starr JM,Tiemeier H,Timpson NJ,Trzaskowski M,Uitterlinden AG,Verhulst FC,Ward ME,Wright MJ,Davey Smith G,Deary IJ,Johannesson M,Plomin R,Visscher PM,Benjamin DJ,Cesarini D,Koellinger PD


book

cDNA cloning of human N-Oct3, a nervous-system specific POU domain transcription factor binding to the octamer DNA motif.
Nucleic acids research21(2)253-8(1993 Jan)
Schreiber E,Tobler A,Malipiero U,Schaffner W,Fontana A


 
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