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EGLN1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol EGLN1
Entrez Gene ID 54583
Full Name egl-9 family hypoxia-inducible factor 1
Synonyms C1orf12, ECYT3, HALAH, HIF-PH2, HIFPH2, HPH-2, HPH2, PHD2, SM20, ZMYND6
General protein information
Preferred Names
egl nine homolog 1
Names
egl nine homolog 1
egl nine-like protein 1
HIF prolyl hydroxylase 2
HIF-prolyl hydroxylase 2
zinc finger MYND domain-containing protein 6
hypoxia-inducible factor prolyl hydroxylase 2
prolyl hydroxylase domain-containing protein 2
NP_071334.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1q42.1

Summary The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3). [provided by RefSeq, Nov 2009]. lac of sum
Disorder MIM:

606425

Disorder Html: Erythrocytosis, familial, 3, 609820 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_005273166 XP_005273223 egl nine homolog 1 isoform X1
XM_005273167 XP_005273224 egl nine homolog 1 isoform X2
NM_022051 NP_071334 egl nine homolog 1



Homo sapiens (human) EGLN1 NP_071334.1
Pan troglodytes (chimpanzee) EGLN1 XP_525092.2
Macaca mulatta (Rhesus monkey) EGLN1 XP_001104870.1
Canis lupus familiaris (dog) EGLN1 XP_546089.3
Mus musculus (house mouse) Egln1 NP_444437.2
Rattus norvegicus (Norway rat) Egln1 XP_002728657.3
Gallus gallus (chicken) LOC768374 XP_001231254.3
Danio rerio (zebrafish) egln1 NP_001002595.2
Danio rerio (zebrafish) si:ch1073-423n4.1 XP_002664281.2
Xenopus (Silurana) tropicalis (western clawed frog) egln1 NP_001015960.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EGLN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EGLN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu35042 XM_005273166 PREDICTED: Homo sapiens egl-9 family hypoxia-inducible factor 1 (EGLN1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $223.30
$319.00
OHu35043 XM_005273167 PREDICTED: Homo sapiens egl-9 family hypoxia-inducible factor 1 (EGLN1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $223.30
$319.00
OHu22997
NM_022051 Homo sapiens egl-9 family hypoxia-inducible factor 1 (EGLN1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$179.50-$251.30
$359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu35042
Clone ID Related Accession (Same CDS sequence) XM_005273166
Accession Version XM_005273166.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1152bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product egl nine homolog 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167186.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578802250. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGCCAATG ACAGCGGCGG GCCCGGCGGG CCGAGCCCGA GCGAGCGAGA CCGGCAGTAC 
TGCGAGCTGT GCGGGAAGAT GGAGAACCTG CTGCGCTGCA GCCGCTGCCG CAGCTCCTTC
TACTGCTGCA AGGAGCACCA GCGTCAGGAC TGGAAGAAGC ACAAGCTCGT GTGCCAGGGC
AGCGAGGGCG CCCTCGGCCA CGGAGTGGGC CCACACCAGC ATTCCGGCCC CGCGCCGCCG
GCTGCAGTGC CGCCGCCCAG GGCCGGGGCC CGGGAGCCCA GGAAGGCAGC GGCGCGCCGG
GACAACGCCT CCGGGGACGC GGCCAAGGGA AAAGTAAAGG CCAAGCCCCC GGCCGACCCA
GCGGCGGCCG CGTCGCCGTG TCGTGCGGCC GCCGGCGGCC AGGGCTCGGC GGTGGCTGCC
GAAGCCGAGC CCGGCAAGGA GGAGCCGCCG GCCCGCTCAT CGCTGTTCCA GGAGAAGGCG
AACCTGTACC CCCCAAGCAA CACGCCCGGG GATGCGCTGA GCCCCGGCGG CGGCCTGCGG
CCCAACGGGC AGACGAAGCC CCTGCCGGCG CTGAAGCTGG CGCTCGAGTA CATCGTGCCG
TGCATGAACA AGCACGGCAT CTGTGTGGTG GACGACTTCC TCGGCAAGGA GACCGGACAG
CAGATCGGCG ACGAGGTGCG CGCCCTGCAC GACACCGGGA AGTTCACGGA CGGGCAGCTG
GTCAGCCAGA AGAGTGACTC GTCCAAGGAC ATCCGAGGCG ATAAGATCAC CTGGATCGAG
GGCAAGGAGC CCGGCTGCGA AACCATTGGG CTGCTCATGA GCAGCATGGA CGACCTGATA
CGCCACTGTA ACGGGAAGCT GGGCAGCTAC AAAATCAATG GCCGGACGAA AGCCATGGTT
GCTTGTTATC CGGGCAATGG AACGGGTTAT GTACGTCATG TTGATAATCC AAATGGAGAT
GGAAGATGTG TGACATGTAT ATATTATCTT AATAAAGACT GGGATGCCAA GGTAAGTGGA
GGTATACTTC GAATTTTTCC AGAAGGCAAA GCCCAGTTTG CTGACATTGA ACCCAAATTT
GATAGACTGC TGTTTTTCTG GTCTGACCGT CGCAACCCTC ATGAAGTACA ACCAGCATAT
GCTACAAGGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005273223.1
CDS429..1580
Misc Feature(1)489..602(+)
Misc Feature(2)1086..1568(+)
Translation

Target ORF information:

RefSeq Version XM_005273166
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens egl-9 family hypoxia-inducible factor 1 (EGLN1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005273166

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGCCAATG ACAGCGGCGG GCCCGGCGGG CCGAGCCCGA GCGAGCGAGA CCGGCAGTAC 
TGCGAGCTGT GCGGGAAGAT GGAGAACCTG CTGCGCTGCA GCCGCTGCCG CAGCTCCTTC
TACTGCTGCA AGGAGCACCA GCGTCAGGAC TGGAAGAAGC ACAAGCTCGT GTGCCAGGGC
AGCGAGGGCG CCCTCGGCCA CGGAGTGGGC CCACACCAGC ATTCCGGCCC CGCGCCGCCG
GCTGCAGTGC CGCCGCCCAG GGCCGGGGCC CGGGAGCCCA GGAAGGCAGC GGCGCGCCGG
GACAACGCCT CCGGGGACGC GGCCAAGGGA AAAGTAAAGG CCAAGCCCCC GGCCGACCCA
GCGGCGGCCG CGTCGCCGTG TCGTGCGGCC GCCGGCGGCC AGGGCTCGGC GGTGGCTGCC
GAAGCCGAGC CCGGCAAGGA GGAGCCGCCG GCCCGCTCAT CGCTGTTCCA GGAGAAGGCG
AACCTGTACC CCCCAAGCAA CACGCCCGGG GATGCGCTGA GCCCCGGCGG CGGCCTGCGG
CCCAACGGGC AGACGAAGCC CCTGCCGGCG CTGAAGCTGG CGCTCGAGTA CATCGTGCCG
TGCATGAACA AGCACGGCAT CTGTGTGGTG GACGACTTCC TCGGCAAGGA GACCGGACAG
CAGATCGGCG ACGAGGTGCG CGCCCTGCAC GACACCGGGA AGTTCACGGA CGGGCAGCTG
GTCAGCCAGA AGAGTGACTC GTCCAAGGAC ATCCGAGGCG ATAAGATCAC CTGGATCGAG
GGCAAGGAGC CCGGCTGCGA AACCATTGGG CTGCTCATGA GCAGCATGGA CGACCTGATA
CGCCACTGTA ACGGGAAGCT GGGCAGCTAC AAAATCAATG GCCGGACGAA AGCCATGGTT
GCTTGTTATC CGGGCAATGG AACGGGTTAT GTACGTCATG TTGATAATCC AAATGGAGAT
GGAAGATGTG TGACATGTAT ATATTATCTT AATAAAGACT GGGATGCCAA GGTAAGTGGA
GGTATACTTC GAATTTTTCC AGAAGGCAAA GCCCAGTTTG CTGACATTGA ACCCAAATTT
GATAGACTGC TGTTTTTCTG GTCTGACCGT CGCAACCCTC ATGAAGTACA ACCAGCATAT
GCTACAAGGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu35043
Clone ID Related Accession (Same CDS sequence) XM_005273167
Accession Version XM_005273167.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1038bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product egl nine homolog 1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167186.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578802251. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCCAATG ACAGCGGCGG GCCCGGCGGG CCGAGCCCGA GCGAGCGAGA CCGGCAGTAC 
TGCGAGCTGT GCGGGAAGAT GGAGAACCTG CTGCGCTGCA GCCGCTGCCG CAGCTCCTTC
TACTGCTGCA AGGAGCACCA GCGTCAGGAC TGGAAGAAGC ACAAGCTCGT GTGCCAGGGC
AGCGAGGGCG CCCTCGGCCA CGGAGTGGGC CCACACCAGC ATTCCGGCCC CGCGCCGCCG
GCTGCAGTGC CGCCGCCCAG GGCCGGGGCC CGGGAGCCCA GGAAGGCAGC GGCGCGCCGG
GACAACGCCT CCGGGGACGC GGCCAAGGGA AAAGTAAAGG CCAAGCCCCC GGCCGACCCA
GCGGCGGCCG CGTCGCCGTG TCGTGCGGCC GCCGGCGGCC AGGGCTCGGC GGTGGCTGCC
GAAGCCGAGC CCGGCAAGGA GGAGCCGCCG GCCCGCTCAT CGCTGTTCCA GGAGAAGGCG
AACCTGTACC CCCCAAGCAA CACGCCCGGG GATGCGCTGA GCCCCGGCGG CGGCCTGCGG
CCCAACGGGC AGACGAAGCC CCTGCCGGCG CTGAAGCTGG CGCTCGAGTA CATCGTGCCG
TGCATGAACA AGCACGGCAT CTGTGTGGTG GACGACTTCC TCGGCAAGGA GACCGGACAG
CAGATCGGCG ACGAGGTGCG CGCCCTGCAC GACACCGGGA AGTTCACGGA CGGGCAGCTG
GTCAGCCAGA AGAGTGACTC GTCCAAGGAC ATCCGAGGCG ATAAGATCAC CTGGATCGAG
GGCAAGGAGC CCGGCTGCGA AACCATTGGG CTGCTCATGA GCAGCATGGA CGACCTGATA
CGCCACTGTA ACGGGAAGCT GGGCAGCTAC AAAATCAATG GCCGGACGAA AGCCATGGTT
GCTTGTTATC CGGGCAATGG AACGGGTTAT GTACGTCATG TTGATAATCC AAATGGAGAT
GGAAGATGTG TGACATGTAT ATATTATCTT AATAAAGACT GGGATGCCAA GGTACGCAAT
AACTGTTTGG TATTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005273224.1
CDS429..1466
Misc Feature(1)489..602(+)
Misc Feature(2)1086..>1427(+)
Translation

Target ORF information:

RefSeq Version XM_005273167
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens egl-9 family hypoxia-inducible factor 1 (EGLN1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005273167

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCCAATG ACAGCGGCGG GCCCGGCGGG CCGAGCCCGA GCGAGCGAGA CCGGCAGTAC 
TGCGAGCTGT GCGGGAAGAT GGAGAACCTG CTGCGCTGCA GCCGCTGCCG CAGCTCCTTC
TACTGCTGCA AGGAGCACCA GCGTCAGGAC TGGAAGAAGC ACAAGCTCGT GTGCCAGGGC
AGCGAGGGCG CCCTCGGCCA CGGAGTGGGC CCACACCAGC ATTCCGGCCC CGCGCCGCCG
GCTGCAGTGC CGCCGCCCAG GGCCGGGGCC CGGGAGCCCA GGAAGGCAGC GGCGCGCCGG
GACAACGCCT CCGGGGACGC GGCCAAGGGA AAAGTAAAGG CCAAGCCCCC GGCCGACCCA
GCGGCGGCCG CGTCGCCGTG TCGTGCGGCC GCCGGCGGCC AGGGCTCGGC GGTGGCTGCC
GAAGCCGAGC CCGGCAAGGA GGAGCCGCCG GCCCGCTCAT CGCTGTTCCA GGAGAAGGCG
AACCTGTACC CCCCAAGCAA CACGCCCGGG GATGCGCTGA GCCCCGGCGG CGGCCTGCGG
CCCAACGGGC AGACGAAGCC CCTGCCGGCG CTGAAGCTGG CGCTCGAGTA CATCGTGCCG
TGCATGAACA AGCACGGCAT CTGTGTGGTG GACGACTTCC TCGGCAAGGA GACCGGACAG
CAGATCGGCG ACGAGGTGCG CGCCCTGCAC GACACCGGGA AGTTCACGGA CGGGCAGCTG
GTCAGCCAGA AGAGTGACTC GTCCAAGGAC ATCCGAGGCG ATAAGATCAC CTGGATCGAG
GGCAAGGAGC CCGGCTGCGA AACCATTGGG CTGCTCATGA GCAGCATGGA CGACCTGATA
CGCCACTGTA ACGGGAAGCT GGGCAGCTAC AAAATCAATG GCCGGACGAA AGCCATGGTT
GCTTGTTATC CGGGCAATGG AACGGGTTAT GTACGTCATG TTGATAATCC AAATGGAGAT
GGAAGATGTG TGACATGTAT ATATTATCTT AATAAAGACT GGGATGCCAA GGTACGCAAT
AACTGTTTGG TATTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22997
Clone ID Related Accession (Same CDS sequence) NM_022051
Accession Version NM_022051.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1281bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product egl nine homolog 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF229245.1, AL117352.12 and AI017372.1. This sequence is a reference standard in the RefSeqGene project. On May 15, 2009 this sequence version replaced gi:13489072. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF229245.1, AJ310543.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGCCAATG ACAGCGGCGG GCCCGGCGGG CCGAGCCCGA GCGAGCGAGA CCGGCAGTAC 
TGCGAGCTGT GCGGGAAGAT GGAGAACCTG CTGCGCTGCA GCCGCTGCCG CAGCTCCTTC
TACTGCTGCA AGGAGCACCA GCGTCAGGAC TGGAAGAAGC ACAAGCTCGT GTGCCAGGGC
AGCGAGGGCG CCCTCGGCCA CGGAGTGGGC CCACACCAGC ATTCCGGCCC CGCGCCGCCG
GCTGCAGTGC CGCCGCCCAG GGCCGGGGCC CGGGAGCCCA GGAAGGCAGC GGCGCGCCGG
GACAACGCCT CCGGGGACGC GGCCAAGGGA AAAGTAAAGG CCAAGCCCCC GGCCGACCCA
GCGGCGGCCG CGTCGCCGTG TCGTGCGGCC GCCGGCGGCC AGGGCTCGGC GGTGGCTGCC
GAAGCCGAGC CCGGCAAGGA GGAGCCGCCG GCCCGCTCAT CGCTGTTCCA GGAGAAGGCG
AACCTGTACC CCCCAAGCAA CACGCCCGGG GATGCGCTGA GCCCCGGCGG CGGCCTGCGG
CCCAACGGGC AGACGAAGCC CCTGCCGGCG CTGAAGCTGG CGCTCGAGTA CATCGTGCCG
TGCATGAACA AGCACGGCAT CTGTGTGGTG GACGACTTCC TCGGCAAGGA GACCGGACAG
CAGATCGGCG ACGAGGTGCG CGCCCTGCAC GACACCGGGA AGTTCACGGA CGGGCAGCTG
GTCAGCCAGA AGAGTGACTC GTCCAAGGAC ATCCGAGGCG ATAAGATCAC CTGGATCGAG
GGCAAGGAGC CCGGCTGCGA AACCATTGGG CTGCTCATGA GCAGCATGGA CGACCTGATA
CGCCACTGTA ACGGGAAGCT GGGCAGCTAC AAAATCAATG GCCGGACGAA AGCCATGGTT
GCTTGTTATC CGGGCAATGG AACGGGTTAT GTACGTCATG TTGATAATCC AAATGGAGAT
GGAAGATGTG TGACATGTAT ATATTATCTT AATAAAGACT GGGATGCCAA GGTAAGTGGA
GGTATACTTC GAATTTTTCC AGAAGGCAAA GCCCAGTTTG CTGACATTGA ACCCAAATTT
GATAGACTGC TGTTTTTCTG GTCTGACCGT CGCAACCCTC ATGAAGTACA ACCAGCATAT
GCTACAAGGT ACGCAATAAC TGTTTGGTAT TTTGATGCAG ATGAGAGAGC ACGAGCTAAA
GTAAAATATC TAACAGGTGA AAAAGGTGTG AGGGTTGAAC TCAATAAACC TTCAGATTCG
GTCGGTAAAG ACGTCTTCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_071334.1
CDS3157..4437
Misc Feature(1)3031..3033(+)
Misc Feature(2)3160..3162(+)
Misc Feature(3)3172..3216(+)
Misc Feature(4)3217..3330(+)
Misc Feature(5)3529..3531(+)
Misc Feature(6)3757..3759(+)
Misc Feature(7)3778..3780(+)
Misc Feature(8)3814..4329(+)
Misc Feature(9)3877..3909(+)
Misc Feature(10)4060..4062(+)
Misc Feature(11)4123..4125(+)
Misc Feature(12)4132..4134(+)
Exon (1)1..4047
Gene:EGLN1
Gene Synonym:
Exon (2)4048..4167
Gene:EGLN1
Gene Synonym:
Exon (3)4168..4304
Gene:EGLN1
Gene Synonym:
Exon (4)4305..4372
Gene:EGLN1
Gene Synonym:
Exon (5)4373..7097
Gene:EGLN1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_022051
Organism Homo sapiens (human)
Definition Homo sapiens egl-9 family hypoxia-inducible factor 1 (EGLN1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_022051

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGCCAATG ACAGCGGCGG GCCCGGCGGG CCGAGCCCGA GCGAGCGAGA CCGGCAGTAC 
TGCGAGCTGT GCGGGAAGAT GGAGAACCTG CTGCGCTGCA GCCGCTGCCG CAGCTCCTTC
TACTGCTGCA AGGAGCACCA GCGTCAGGAC TGGAAGAAGC ACAAGCTCGT GTGCCAGGGC
AGCGAGGGCG CCCTCGGCCA CGGAGTGGGC CCACACCAGC ATTCCGGCCC CGCGCCGCCG
GCTGCAGTGC CGCCGCCCAG GGCCGGGGCC CGGGAGCCCA GGAAGGCAGC GGCGCGCCGG
GACAACGCCT CCGGGGACGC GGCCAAGGGA AAAGTAAAGG CCAAGCCCCC GGCCGACCCA
GCGGCGGCCG CGTCGCCGTG TCGTGCGGCC GCCGGCGGCC AGGGCTCGGC GGTGGCTGCC
GAAGCCGAGC CCGGCAAGGA GGAGCCGCCG GCCCGCTCAT CGCTGTTCCA GGAGAAGGCG
AACCTGTACC CCCCAAGCAA CACGCCCGGG GATGCGCTGA GCCCCGGCGG CGGCCTGCGG
CCCAACGGGC AGACGAAGCC CCTGCCGGCG CTGAAGCTGG CGCTCGAGTA CATCGTGCCG
TGCATGAACA AGCACGGCAT CTGTGTGGTG GACGACTTCC TCGGCAAGGA GACCGGACAG
CAGATCGGCG ACGAGGTGCG CGCCCTGCAC GACACCGGGA AGTTCACGGA CGGGCAGCTG
GTCAGCCAGA AGAGTGACTC GTCCAAGGAC ATCCGAGGCG ATAAGATCAC CTGGATCGAG
GGCAAGGAGC CCGGCTGCGA AACCATTGGG CTGCTCATGA GCAGCATGGA CGACCTGATA
CGCCACTGTA ACGGGAAGCT GGGCAGCTAC AAAATCAATG GCCGGACGAA AGCCATGGTT
GCTTGTTATC CGGGCAATGG AACGGGTTAT GTACGTCATG TTGATAATCC AAATGGAGAT
GGAAGATGTG TGACATGTAT ATATTATCTT AATAAAGACT GGGATGCCAA GGTAAGTGGA
GGTATACTTC GAATTTTTCC AGAAGGCAAA GCCCAGTTTG CTGACATTGA ACCCAAATTT
GATAGACTGC TGTTTTTCTG GTCTGACCGT CGCAACCCTC ATGAAGTACA ACCAGCATAT
GCTACAAGGT ACGCAATAAC TGTTTGGTAT TTTGATGCAG ATGAGAGAGC ACGAGCTAAA
GTAAAATATC TAACAGGTGA AAAAGGTGTG AGGGTTGAAC TCAATAAACC TTCAGATTCG
GTCGGTAAAG ACGTCTTCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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