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POU5F1 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol POU5F1
Entrez Gene ID 5460
Full Name POU class 5 homeobox 1
Synonyms OCT3,OCT4,OTF-3,OTF3,OTF4,Oct-3,Oct-4
General protein information
Preferred Names

POU class 5 homeobox 1

Names

POU domain, class 5, transcription factor 1
POU class 5 homeobox 1 transcript variant OCT4B1
POU class 5 homeobox 1 transcript variant OCT4B2
POU class 5 homeobox 1 transcript variant OCT4B3
POU class 5 homeobox 1 transcript variant OCT4B4
POU class 5 homeobox 1 transcript variant OCT4B5
POU class 5 homeobox 1 transcript variant OCT4B6
POU classV homeobox 1 variant 2
POU domain transcription factor OCT4
POU-type homeodomain-containing DNA-binding protein
octamer-binding protein 3
octamer-binding protein 4
octamer-binding transcription factor 3

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

6

6p21.33

Summary This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013].
Disorder MIM:

164177


mRNA and Protein(s)

mRNA Protein Name
NM_001285986.1 NP_001272915.1 POU domain, class 5, transcription factor 1 isoform 4
NM_001285987.1 NP_001272916.1 POU domain, class 5, transcription factor 1 isoform 3
NM_001173531.2 NP_001167002.1 POU domain, class 5, transcription factor 1 isoform 2
NM_203289.5 NP_976034.4 POU domain, class 5, transcription factor 1 isoform 2
NM_002701.5 NP_002692.2 POU domain, class 5, transcription factor 1 isoform 1



Homo sapiens (human) POU5F1 NP_002692.2
Pan troglodytes (chimpanzee) POU5F1 NP_001238970.1
Bos taurus (cattle) POU5F1 NP_777005.1
Canis lupus familiaris (dog) POU5F1 XP_538830.1
Mus musculus (house mouse) Pou5f1 NP_038661.2
Macaca mulatta (rhesus monkey) POU5F1 NP_001108427.1
Rattus norvegicus (Norway rat) Pou5f1 NP_001009178.1


Related articles in PubMed

Distinctive expression pattern of OCT4 variants in different types of breast cancer.
Soheili S, Asadi MH, Farsinejad A
Cancer biomarkers : section A of Disease markers18(1)69-76(2017)

Enhanced expression of the stemness-related factors OCT4, SOX15 and TWIST1 in ectopic endometrium of endometriosis patients.
Proestling K, Birner P, Balendran S, Nirtl N, Marton E, Yerlikaya G, Kuessel L, Reischer T, Wenzl R, Streubel B, Husslein H
Reproductive biology and endocrinology : RB&E14(1)81(2016 Nov)

Concise review: isoforms of OCT4 contribute to the confusing diversity in stem cell biology.
Wang X, Dai J
Stem cells (Dayton, Ohio)28(5)885-93(2010 May)

Mapping of the minimal internal ribosome entry site element in the human embryonic stem cell gene OCT4B mRNA.
Zhang W, Wang X, Xiao Z, Liu W, Chen B, Dai J
Biochemical and biophysical research communications394(3)750-4(2010 Apr)

Alternative translation of OCT4 by an internal ribosome entry site and its novel function in stress response.
Wang X, Zhao Y, Xiao Z, Chen B, Wei Z, Wang B, Zhang J, Han J, Gao Y, Li L, Zhao H, Zhao W, Lin H, Dai J
Stem cells (Dayton, Ohio)27(6)1265-75(2009 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the early response of pluripotency genes OCT4 and NANOG to the differentiation-inducing stimuli is mediated by dynamic changes in chromatin marks, while DNA methylation is acquired in the later stages of neurogenesis.
Title: Histone Modifications on the Promoters of Human OCT4 and NANOG Genes at the Onset of Neural Differentiation of NT2/D1 Cells.

Data show that Nanog homebox (NANOG) but not sex-determining region Y-box2 (SOX2) and octamer-binding protein 4 (OCT4) expression was overexpressed in the endometrium of women with intrauterine adhesion (IUA).
Title: Expression of SOX2, NANOG and OCT4 in a mouse model of lipopolysaccharide-induced acute uterine injury and intrauterine adhesions.

Increased expression of epithelial OCT4, SOX15 and TWIST1 was detected in endometriotic tissue compared to endometriosis patient endometrium. Epithelial expression of OCT4, SOX15, and TWIST1 was increased in the endometrium of endometriosis patients compared to controls.
Title: Enhanced expression of the stemness-related factors OCT4, SOX15 and TWIST1 in ectopic endometrium of endometriosis patients.

High Oct-4 expression was associated with Astrocytic Brain Tumors.
Title: Expression and Prognostic Value of Oct-4 in Astrocytic Brain Tumors.

OCT4 plays a role as a transcriptional activator for GDF3 transcription in pluripotent human embryonic carcinoma NCCIT cells and contributes to the understanding of the molecular networks of stem cell regulators in germ cell-derived pluripotency and tumorigenesis.
Title: Growth and Differentiation Factor 3 Is Transcriptionally Regulated by OCT4 in Human Embryonic Carcinoma Cells.

The following POU5F1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POU5F1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu20361
NM_002701.5
Latest version!
Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu20662 NM_001173531.2
Latest version!
Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $69.30
$99.00
OHu20662 NM_203289.5
Latest version!
Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $69.30
$99.00
OHu21050 NM_001285987.1
Latest version!
Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu21117 NM_001285986.1
Latest version!
Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu20361
Clone ID Related Accession (Same CDS sequence) NM_002701.5
Accession Version NM_002701.5 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1083bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-09
Organism Homo sapiens(Human)
Product POU domain, class 5, transcription factor 1 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD247724.1, BC117435.1, DQ486517.1 and AI811039.1. On Oct 26, 2013 this sequence version replaced NM_002701.4. Transcript Variant: This variant (1, also known as OCT4A) represents the shortest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Z11898.1, BC069246.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCGGGAC ACCTGGCTTC GGATTTCGCC TTCTCGCCCC CTCCAGGTGG TGGAGGTGAT 
GGGCCAGGGG GGCCGGAGCC GGGCTGGGTT GATCCTCGGA CCTGGCTAAG CTTCCAAGGC
CCTCCTGGAG GGCCAGGAAT CGGGCCGGGG GTTGGGCCAG GCTCTGAGGT GTGGGGGATT
CCCCCATGCC CCCCGCCGTA TGAGTTCTGT GGGGGGATGG CGTACTGTGG GCCCCAGGTT
GGAGTGGGGC TAGTGCCCCA AGGCGGCTTG GAGACCTCTC AGCCTGAGGG CGAAGCAGGA
GTCGGGGTGG AGAGCAACTC CGATGGGGCC TCCCCGGAGC CCTGCACCGT CACCCCTGGT
GCCGTGAAGC TGGAGAAGGA GAAGCTGGAG CAAAACCCGG AGGAGTCCCA GGACATCAAA
GCTCTGCAGA AAGAACTCGA GCAATTTGCC AAGCTCCTGA AGCAGAAGAG GATCACCCTG
GGATATACAC AGGCCGATGT GGGGCTCACC CTGGGGGTTC TATTTGGGAA GGTATTCAGC
CAAACGACCA TCTGCCGCTT TGAGGCTCTG CAGCTTAGCT TCAAGAACAT GTGTAAGCTG
CGGCCCTTGC TGCAGAAGTG GGTGGAGGAA GCTGACAACA ATGAAAATCT TCAGGAGATA
TGCAAAGCAG AAACCCTCGT GCAGGCCCGA AAGAGAAAGC GAACCAGTAT CGAGAACCGA
GTGAGAGGCA ACCTGGAGAA TTTGTTCCTG CAGTGCCCGA AACCCACACT GCAGCAGATC
AGCCACATCG CCCAGCAGCT TGGGCTCGAG AAGGATGTGG TCCGAGTGTG GTTCTGTAAC
CGGCGCCAGA AGGGCAAGCG ATCAAGCAGC GACTATGCAC AACGAGAGGA TTTTGAGGCT
GCTGGGTCTC CTTTCTCAGG GGGACCAGTG TCCTTTCCTC TGGCCCCAGG GCCCCATTTT
GGTACCCCAG GCTATGGGAG CCCTCACTTC ACTGCACTGT ACTCCTCGGT CCCTTTCCCT
GAGGGGGAAG CCTTTCCCCC TGTCTCCGTC ACCACTCTGG GCTCTCCCAT GCATTCAAAC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002692.2
CDS74..1156
Misc Feature(1)11..13
Misc Feature(2)404..406
Misc Feature(3)485..709
Misc Feature(4)order(764..778,782..784,833..835,851..853,890..892,896..901,908..913,917..925,929..934)
Misc Feature(5)770..931
Misc Feature(6)order(770..772,779..781,899..901,908..913,920..922)
Misc Feature(7)776..778
Misc Feature(8)779..781
Misc Feature(9)938..940
Misc Feature(10)941..943
Misc Feature(11)1136..1138
Exon (1)1..478
Exon (2)479..599
Exon (3)600..730
Exon (4)731..889
Exon (5)890..1420
Translation

Target ORF information:

RefSeq Version NM_002701.5
Organism Homo sapiens(Human)
Definition Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002701.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCGGGAC ACCTGGCTTC GGATTTCGCC TTCTCGCCCC CTCCAGGTGG TGGAGGTGAT 
GGGCCAGGGG GGCCGGAGCC GGGCTGGGTT GATCCTCGGA CCTGGCTAAG CTTCCAAGGC
CCTCCTGGAG GGCCAGGAAT CGGGCCGGGG GTTGGGCCAG GCTCTGAGGT GTGGGGGATT
CCCCCATGCC CCCCGCCGTA TGAGTTCTGT GGGGGGATGG CGTACTGTGG GCCCCAGGTT
GGAGTGGGGC TAGTGCCCCA AGGCGGCTTG GAGACCTCTC AGCCTGAGGG CGAAGCAGGA
GTCGGGGTGG AGAGCAACTC CGATGGGGCC TCCCCGGAGC CCTGCACCGT CACCCCTGGT
GCCGTGAAGC TGGAGAAGGA GAAGCTGGAG CAAAACCCGG AGGAGTCCCA GGACATCAAA
GCTCTGCAGA AAGAACTCGA GCAATTTGCC AAGCTCCTGA AGCAGAAGAG GATCACCCTG
GGATATACAC AGGCCGATGT GGGGCTCACC CTGGGGGTTC TATTTGGGAA GGTATTCAGC
CAAACGACCA TCTGCCGCTT TGAGGCTCTG CAGCTTAGCT TCAAGAACAT GTGTAAGCTG
CGGCCCTTGC TGCAGAAGTG GGTGGAGGAA GCTGACAACA ATGAAAATCT TCAGGAGATA
TGCAAAGCAG AAACCCTCGT GCAGGCCCGA AAGAGAAAGC GAACCAGTAT CGAGAACCGA
GTGAGAGGCA ACCTGGAGAA TTTGTTCCTG CAGTGCCCGA AACCCACACT GCAGCAGATC
AGCCACATCG CCCAGCAGCT TGGGCTCGAG AAGGATGTGG TCCGAGTGTG GTTCTGTAAC
CGGCGCCAGA AGGGCAAGCG ATCAAGCAGC GACTATGCAC AACGAGAGGA TTTTGAGGCT
GCTGGGTCTC CTTTCTCAGG GGGACCAGTG TCCTTTCCTC TGGCCCCAGG GCCCCATTTT
GGTACCCCAG GCTATGGGAG CCCTCACTTC ACTGCACTGT ACTCCTCGGT CCCTTTCCCT
GAGGGGGAAG CCTTTCCCCC TGTCTCCGTC ACCACTCTGG GCTCTCCCAT GCATTCAAAC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20662
Clone ID Related Accession (Same CDS sequence) NM_001173531.2 , NM_203289.5
Accession Version NM_001173531.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 573bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-30
Organism Homo sapiens(Human)
Product POU domain, class 5, transcription factor 1 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ486514.1, DQ486516.1, DQ486515.1 and AI811039.1. On Oct 26, 2013 this sequence version replaced NM_001173531.1. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream in-frame non-AUG (CUG) start codon, compared to variant 1. The resulting isoform (2, also known as OCT4B-190) is shorter at the N-terminus, compared to isoform 1. Variants 2 and 3 encode the same isoform (2). This variant may encode an additional isoform through the use of an alternative downstream AUG start codon. Use of alternate start codons and the non-AUG start codon is described in PMID:19489092. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: KF880691.1, DQ486516.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2150385, SAMEA2157511 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 19489092 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
CTGGGGGTTC TATTTGGGAA GGTATTCAGC CAAACGACCA TCTGCCGCTT TGAGGCTCTG 
CAGCTTAGCT TCAAGAACAT GTGTAAGCTG CGGCCCTTGC TGCAGAAGTG GGTGGAGGAA
GCTGACAACA ATGAAAATCT TCAGGAGATA TGCAAAGCAG AAACCCTCGT GCAGGCCCGA
AAGAGAAAGC GAACCAGTAT CGAGAACCGA GTGAGAGGCA ACCTGGAGAA TTTGTTCCTG
CAGTGCCCGA AACCCACACT GCAGCAGATC AGCCACATCG CCCAGCAGCT TGGGCTCGAG
AAGGATGTGG TCCGAGTGTG GTTCTGTAAC CGGCGCCAGA AGGGCAAGCG ATCAAGCAGC
GACTATGCAC AACGAGAGGA TTTTGAGGCT GCTGGGTCTC CTTTCTCAGG GGGACCAGTG
TCCTTTCCTC TGGCCCCAGG GCCCCATTTT GGTACCCCAG GCTATGGGAG CCCTCACTTC
ACTGCACTGT ACTCCTCGGT CCCTTTCCCT GAGGGGGAAG CCTTTCCCCC TGTCTCCGTC
ACCACTCTGG GCTCTCCCAT GCATTCAAAC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001167002.1
CDS743..1315
Misc Feature(1)623..625
Misc Feature(2)<743..868
Misc Feature(3)821..823
Misc Feature(4)order(923..937,941..943,992..994,1010..1012,1049..1051,1055..1060,1067..1072,1076..1084,1088..1093)
Misc Feature(5)929..1090
Misc Feature(6)order(929..931,938..940,1058..1060,1067..1072,1079..1081)
Exon (1)1..637
Exon (2)638..758
Exon (3)759..889
Exon (4)890..1048
Exon (5)1049..1579
Translation

Target ORF information:

RefSeq Version NM_001173531.2
Organism Homo sapiens(Human)
Definition Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001173531.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
CTGGGGGTTC TATTTGGGAA GGTATTCAGC CAAACGACCA TCTGCCGCTT TGAGGCTCTG 
CAGCTTAGCT TCAAGAACAT GTGTAAGCTG CGGCCCTTGC TGCAGAAGTG GGTGGAGGAA
GCTGACAACA ATGAAAATCT TCAGGAGATA TGCAAAGCAG AAACCCTCGT GCAGGCCCGA
AAGAGAAAGC GAACCAGTAT CGAGAACCGA GTGAGAGGCA ACCTGGAGAA TTTGTTCCTG
CAGTGCCCGA AACCCACACT GCAGCAGATC AGCCACATCG CCCAGCAGCT TGGGCTCGAG
AAGGATGTGG TCCGAGTGTG GTTCTGTAAC CGGCGCCAGA AGGGCAAGCG ATCAAGCAGC
GACTATGCAC AACGAGAGGA TTTTGAGGCT GCTGGGTCTC CTTTCTCAGG GGGACCAGTG
TCCTTTCCTC TGGCCCCAGG GCCCCATTTT GGTACCCCAG GCTATGGGAG CCCTCACTTC
ACTGCACTGT ACTCCTCGGT CCCTTTCCCT GAGGGGGAAG CCTTTCCCCC TGTCTCCGTC
ACCACTCTGG GCTCTCCCAT GCATTCAAAC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20662
Clone ID Related Accession (Same CDS sequence) NM_001173531.2 , NM_203289.5
Accession Version NM_203289.5 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 573bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-30
Organism Homo sapiens(Human)
Product POU domain, class 5, transcription factor 1 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ486514.1, DQ486515.1 and AI811039.1. On Oct 26, 2013 this sequence version replaced NM_203289.4. Transcript Variant: This variant (2, also known as OCT4B) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream in-frame non-AUG (CUG) start codon, compared to variant 1. The resulting isoform (2, also known as OCT4B-190) is shorter at the N-terminus, compared to isoform 1. Variants 2 and 3 encode the same isoform (2). This variant may encode additional isoforms through the use of an alternative downstream AUG start codon, as well as an alternative upstream AUG start codon, which is polymorphic in human populations (AGG allele represented in this RefSeq; see rs3130932). Use of alternate start codons and the non-AUG start codon is described in PMID:19489092. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: KX151172.1, DQ486515.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 19489092 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
CTGGGGGTTC TATTTGGGAA GGTATTCAGC CAAACGACCA TCTGCCGCTT TGAGGCTCTG 
CAGCTTAGCT TCAAGAACAT GTGTAAGCTG CGGCCCTTGC TGCAGAAGTG GGTGGAGGAA
GCTGACAACA ATGAAAATCT TCAGGAGATA TGCAAAGCAG AAACCCTCGT GCAGGCCCGA
AAGAGAAAGC GAACCAGTAT CGAGAACCGA GTGAGAGGCA ACCTGGAGAA TTTGTTCCTG
CAGTGCCCGA AACCCACACT GCAGCAGATC AGCCACATCG CCCAGCAGCT TGGGCTCGAG
AAGGATGTGG TCCGAGTGTG GTTCTGTAAC CGGCGCCAGA AGGGCAAGCG ATCAAGCAGC
GACTATGCAC AACGAGAGGA TTTTGAGGCT GCTGGGTCTC CTTTCTCAGG GGGACCAGTG
TCCTTTCCTC TGGCCCCAGG GCCCCATTTT GGTACCCCAG GCTATGGGAG CCCTCACTTC
ACTGCACTGT ACTCCTCGGT CCCTTTCCCT GAGGGGGAAG CCTTTCCCCC TGTCTCCGTC
ACCACTCTGG GCTCTCCCAT GCATTCAAAC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_976034.4
CDS1229..1801
Misc Feature(1)974..976
Misc Feature(2)1004..1006
Misc Feature(3)<1229..1354
Misc Feature(4)1307..1309
Misc Feature(5)order(1409..1423,1427..1429,1478..1480,1496..1498,1535..1537,1541..1546,1553..1558,1562..1570,1574..1579)
Misc Feature(6)1415..1576
Misc Feature(7)order(1415..1417,1424..1426,1544..1546,1553..1558,1565..1567)
Exon (1)1..1244
Exon (2)1245..1375
Exon (3)1376..1534
Exon (4)1535..2065
Translation

Target ORF information:

RefSeq Version NM_203289.5
Organism Homo sapiens(Human)
Definition Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_203289.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
CTGGGGGTTC TATTTGGGAA GGTATTCAGC CAAACGACCA TCTGCCGCTT TGAGGCTCTG 
CAGCTTAGCT TCAAGAACAT GTGTAAGCTG CGGCCCTTGC TGCAGAAGTG GGTGGAGGAA
GCTGACAACA ATGAAAATCT TCAGGAGATA TGCAAAGCAG AAACCCTCGT GCAGGCCCGA
AAGAGAAAGC GAACCAGTAT CGAGAACCGA GTGAGAGGCA ACCTGGAGAA TTTGTTCCTG
CAGTGCCCGA AACCCACACT GCAGCAGATC AGCCACATCG CCCAGCAGCT TGGGCTCGAG
AAGGATGTGG TCCGAGTGTG GTTCTGTAAC CGGCGCCAGA AGGGCAAGCG ATCAAGCAGC
GACTATGCAC AACGAGAGGA TTTTGAGGCT GCTGGGTCTC CTTTCTCAGG GGGACCAGTG
TCCTTTCCTC TGGCCCCAGG GCCCCATTTT GGTACCCCAG GCTATGGGAG CCCTCACTTC
ACTGCACTGT ACTCCTCGGT CCCTTTCCCT GAGGGGGAAG CCTTTCCCCC TGTCTCCGTC
ACCACTCTGG GCTCTCCCAT GCATTCAAAC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21050
Clone ID Related Accession (Same CDS sequence) NM_001285987.1
Accession Version NM_001285987.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 798bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-30
Organism Homo sapiens(Human)
Product POU domain, class 5, transcription factor 1 isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ486514.1, DQ486515.1, Z11899.1 and AI811039.1. Transcript Variant: This variant (5, also known as OCT4B) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate AUG start codon, compared to variant 1. The resulting isoform (3, also known as OCT4B-265) is shorter and has a distinct N-terminus, compared to isoform 1. This variant represents an allele of variant 2 that contains an AUG start codon that is polymorphic in human populations (see rs3130932). This variant may encode additional isoforms through the use of alternative downstream AUG and non-AUG start codons, as described in PMID:19489092. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: KX151172.1, DQ486515.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGCACTTCT ACAGACTATT CCTTGGGGCC ACACGTAGGT TCTTGAATCC CGAATGGAAA 
GGGGAGATTG ATAACTGGTG TGTTTATGTT CTTACAAGTC TTCTGCCTTT TAAAATCCAG
TCCCAGGACA TCAAAGCTCT GCAGAAAGAA CTCGAGCAAT TTGCCAAGCT CCTGAAGCAG
AAGAGGATCA CCCTGGGATA TACACAGGCC GATGTGGGGC TCACCCTGGG GGTTCTATTT
GGGAAGGTAT TCAGCCAAAC GACCATCTGC CGCTTTGAGG CTCTGCAGCT TAGCTTCAAG
AACATGTGTA AGCTGCGGCC CTTGCTGCAG AAGTGGGTGG AGGAAGCTGA CAACAATGAA
AATCTTCAGG AGATATGCAA AGCAGAAACC CTCGTGCAGG CCCGAAAGAG AAAGCGAACC
AGTATCGAGA ACCGAGTGAG AGGCAACCTG GAGAATTTGT TCCTGCAGTG CCCGAAACCC
ACACTGCAGC AGATCAGCCA CATCGCCCAG CAGCTTGGGC TCGAGAAGGA TGTGGTCCGA
GTGTGGTTCT GTAACCGGCG CCAGAAGGGC AAGCGATCAA GCAGCGACTA TGCACAACGA
GAGGATTTTG AGGCTGCTGG GTCTCCTTTC TCAGGGGGAC CAGTGTCCTT TCCTCTGGCC
CCAGGGCCCC ATTTTGGTAC CCCAGGCTAT GGGAGCCCTC ACTTCACTGC ACTGTACTCC
TCGGTCCCTT TCCCTGAGGG GGAAGCCTTT CCCCCTGTCT CCGTCACCAC TCTGGGCTCT
CCCATGCATT CAAACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001272916.1
CDS1004..1801
Misc Feature(1)974..976
Misc Feature(2)1130..1354
Misc Feature(3)1229..1231
Misc Feature(4)1307..1309
Misc Feature(5)order(1409..1423,1427..1429,1478..1480,1496..1498,1535..1537,1541..1546,1553..1558,1562..1570,1574..1579)
Misc Feature(6)1415..1576
Misc Feature(7)order(1415..1417,1424..1426,1544..1546,1553..1558,1565..1567)
Exon (1)1..1244
Exon (2)1245..1375
Exon (3)1376..1534
Exon (4)1535..2065
Translation

Target ORF information:

RefSeq Version NM_001285987.1
Organism Homo sapiens(Human)
Definition Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001285987.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGCACTTCT ACAGACTATT CCTTGGGGCC ACACGTAGGT TCTTGAATCC CGAATGGAAA 
GGGGAGATTG ATAACTGGTG TGTTTATGTT CTTACAAGTC TTCTGCCTTT TAAAATCCAG
TCCCAGGACA TCAAAGCTCT GCAGAAAGAA CTCGAGCAAT TTGCCAAGCT CCTGAAGCAG
AAGAGGATCA CCCTGGGATA TACACAGGCC GATGTGGGGC TCACCCTGGG GGTTCTATTT
GGGAAGGTAT TCAGCCAAAC GACCATCTGC CGCTTTGAGG CTCTGCAGCT TAGCTTCAAG
AACATGTGTA AGCTGCGGCC CTTGCTGCAG AAGTGGGTGG AGGAAGCTGA CAACAATGAA
AATCTTCAGG AGATATGCAA AGCAGAAACC CTCGTGCAGG CCCGAAAGAG AAAGCGAACC
AGTATCGAGA ACCGAGTGAG AGGCAACCTG GAGAATTTGT TCCTGCAGTG CCCGAAACCC
ACACTGCAGC AGATCAGCCA CATCGCCCAG CAGCTTGGGC TCGAGAAGGA TGTGGTCCGA
GTGTGGTTCT GTAACCGGCG CCAGAAGGGC AAGCGATCAA GCAGCGACTA TGCACAACGA
GAGGATTTTG AGGCTGCTGG GTCTCCTTTC TCAGGGGGAC CAGTGTCCTT TCCTCTGGCC
CCAGGGCCCC ATTTTGGTAC CCCAGGCTAT GGGAGCCCTC ACTTCACTGC ACTGTACTCC
TCGGTCCCTT TCCCTGAGGG GGAAGCCTTT CCCCCTGTCT CCGTCACCAC TCTGGGCTCT
CCCATGCATT CAAACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21117
Clone ID Related Accession (Same CDS sequence) NM_001285986.1
Accession Version NM_001285986.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 495bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-30
Organism Homo sapiens(Human)
Product POU domain, class 5, transcription factor 1 isoform 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ486514.1, DQ486515.1 and AI811039.1. Transcript Variant: This variant (4, also known as OCT4B1) contains multiple differences in the 5' UTR and the 5' coding region, compared to variant 1, and initiates translation at a downstream in-frame AUG start codon. The resulting isoform (4, also known as OCT4B-164) is shorter at the N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: KF880692.1, DR158398.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
ATGTGTAAGC TGCGGCCCTT GCTGCAGAAG TGGGTGGAGG AAGCTGACAA CAATGAAAAT 
CTTCAGGAGA TATGCAAAGC AGAAACCCTC GTGCAGGCCC GAAAGAGAAA GCGAACCAGT
ATCGAGAACC GAGTGAGAGG CAACCTGGAG AATTTGTTCC TGCAGTGCCC GAAACCCACA
CTGCAGCAGA TCAGCCACAT CGCCCAGCAG CTTGGGCTCG AGAAGGATGT GGTCCGAGTG
TGGTTCTGTA ACCGGCGCCA GAAGGGCAAG CGATCAAGCA GCGACTATGC ACAACGAGAG
GATTTTGAGG CTGCTGGGTC TCCTTTCTCA GGGGGACCAG TGTCCTTTCC TCTGGCCCCA
GGGCCCCATT TTGGTACCCC AGGCTATGGG AGCCCTCACT TCACTGCACT GTACTCCTCG
GTCCCTTTCC CTGAGGGGGA AGCCTTTCCC CCTGTCTCCG TCACCACTCT GGGCTCTCCC
ATGCATTCAA ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001272915.1
CDS1532..2026
Misc Feature(1)1349..1351
Misc Feature(2)<1532..1579
Misc Feature(3)order(1634..1648,1652..1654,1703..1705,1721..1723,1760..1762,1766..1771,1778..1783,1787..1795,1799..1804)
Misc Feature(4)1640..1801
Misc Feature(5)order(1640..1642,1649..1651,1769..1771,1778..1783,1790..1792)
Exon (1)1..1600
Exon (2)1601..1759
Exon (3)1760..2290
Translation

Target ORF information:

RefSeq Version NM_001285986.1
Organism Homo sapiens(Human)
Definition Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001285986.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGTGTAAGC TGCGGCCCTT GCTGCAGAAG TGGGTGGAGG AAGCTGACAA CAATGAAAAT 
CTTCAGGAGA TATGCAAAGC AGAAACCCTC GTGCAGGCCC GAAAGAGAAA GCGAACCAGT
ATCGAGAACC GAGTGAGAGG CAACCTGGAG AATTTGTTCC TGCAGTGCCC GAAACCCACA
CTGCAGCAGA TCAGCCACAT CGCCCAGCAG CTTGGGCTCG AGAAGGATGT GGTCCGAGTG
TGGTTCTGTA ACCGGCGCCA GAAGGGCAAG CGATCAAGCA GCGACTATGC ACAACGAGAG
GATTTTGAGG CTGCTGGGTC TCCTTTCTCA GGGGGACCAG TGTCCTTTCC TCTGGCCCCA
GGGCCCCATT TTGGTACCCC AGGCTATGGG AGCCCTCACT TCACTGCACT GTACTCCTCG
GTCCCTTTCC CTGAGGGGGA AGCCTTTCCC CCTGTCTCCG TCACCACTCT GGGCTCTCCC
ATGCATTCAA ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Distinctive expression pattern of OCT4 variants in different types of breast cancer.
Cancer biomarkers : section A of Disease markers18(1)69-76(2017)
Soheili S,Asadi MH,Farsinejad A


book

Enhanced expression of the stemness-related factors OCT4, SOX15 and TWIST1 in ectopic endometrium of endometriosis patients.
Reproductive biology and endocrinology : RB&E14(1)81(2016 Nov)
Proestling K,Birner P,Balendran S,Nirtl N,Marton E,Yerlikaya G,Kuessel L,Reischer T,Wenzl R,Streubel B,Husslein H


book

Concise review: isoforms of OCT4 contribute to the confusing diversity in stem cell biology.
Stem cells (Dayton, Ohio)28(5)885-93(2010 May)
Wang X,Dai J


book

Mapping of the minimal internal ribosome entry site element in the human embryonic stem cell gene OCT4B mRNA.
Biochemical and biophysical research communications394(3)750-4(2010 Apr)
Zhang W,Wang X,Xiao Z,Liu W,Chen B,Dai J


book

Alternative translation of OCT4 by an internal ribosome entry site and its novel function in stress response.
Stem cells (Dayton, Ohio)27(6)1265-75(2009 Jun)
Wang X,Zhao Y,Xiao Z,Chen B,Wei Z,Wang B,Zhang J,Han J,Gao Y,Li L,Zhao H,Zhao W,Lin H,Dai J


 
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