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PPAT cDNA ORF clone, Homo sapiens (human)

Gene Symbol PPAT
Entrez Gene ID 5471
Full Name phosphoribosyl pyrophosphate amidotransferase
Synonyms ATASE, GPAT, PRAT
General protein information
Preferred Names
amidophosphoribosyltransferase
Names
amidophosphoribosyltransferase
glutamine PRPP amidotransferase
glutamine phosphoribosylpyrophosphate amidotransferase
glutamine phosphoribosylpyrophosphatate amidotransferase
NP_002694.3
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

4

4q12

Summary The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region. [provided by RefSeq, Mar 2011]. lac of sum
Disorder MIM:

172450


mRNA and Protein(s)

mRNA Protein Name
XM_011534387 XP_011532689 amidophosphoribosyltransferase isoform X1
NM_002703 NP_002694 amidophosphoribosyltransferase proprotein



Homo sapiens (human) PPAT NP_002694.3
Pan troglodytes (chimpanzee) PPAT XP_001140991.1
Macaca mulatta (Rhesus monkey) PPAT XP_001085606.1
Canis lupus familiaris (dog) PPAT XP_854170.1
Bos taurus (cattle) PPAT NP_001095845.1
Mus musculus (house mouse) Ppat NP_742158.1
Rattus norvegicus (Norway rat) Ppat NP_476546.1
Gallus gallus (chicken) PPAT NP_001004401.1
Danio rerio (zebrafish) ppat NP_001076346.2
Drosophila melanogaster (fruit fly) Prat2 NP_523949.2
Drosophila melanogaster (fruit fly) Prat NP_524271.2
Caenorhabditis elegans CELE_T04A8.5 NP_497958.1
Arabidopsis thaliana (thale cress) ASE3 NP_195599.1
Arabidopsis thaliana (thale cress) ASE1 NP_179247.1
Arabidopsis thaliana (thale cress) ASE2 NP_195200.1
Xenopus (Silurana) tropicalis (western clawed frog) ppat NP_989313.1


The following PPAT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPAT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu59843 XM_011534387 PREDICTED: Homo sapiens phosphoribosyl pyrophosphate amidotransferase (PPAT), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu03961
NM_002703 Homo sapiens phosphoribosyl pyrophosphate amidotransferase (PPAT), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$219.50-$307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu59843
Clone ID Related Accession (Same CDS sequence) XM_011534387
Accession Version XM_011534387.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1311bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product amidophosphoribosyltransferase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022853.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGGAGTTC GGTGCCAACA TTCAAATCAC ACAAGGTATG CCACCACAGG AAAATGTGAA 
CTAGAAAATT GTCAGCCCTT CGTTGTTGAA ACACTTCATG GGAAGATAGC TGTGGCACAT
AATGGCGAAT TGGTAAATGC TGCTCGATTA AGGAAAAAGC TTCTGCGTCA TGGTATTGGT
CTGTCTACAA GTTCTGATAG TGAAATGATT ACCCAGTTAC TGGCGTATAC CCCTCCTCAG
GAACAAGATG ACACCCCAGA CTGGGTAGCC AGGATTAAAA ACTTGATGAA GGAAGCACCC
ACAGCATACT CCCTGCTTAT AATGCACAGA GATGTTATTT ATGCAGTACG AGATCCTTAT
GGAAATCGTC CCTTATGCAT TGGTCGTCTT ATTCCAGTGT CTGATATAAA TGACAAAGAG
AAAAAAACAT CAGAAACAGA AGGATGGGTG GTGTCTTCAG AATCTTGTAG CTTCTTATCT
ATTGGTGCAA GATATTACCG TGAAGTCTTG CCTGGAGAAA TTGTGGAAAT ATCCAGACAC
AATGTCCAAA CTCTTGATAT TATATCAAGG TCTGAAGGAA ACCCAGTGGC TTTTTGTATC
TTTGAATATG TTTATTTTGC AAGACCAGAC AGTATGTTCG AAGACCAAAT GGTTTATACA
GTAAGATACC GTTGTGGCCA GCAGCTAGCG ATTGAAGCAC CTGTGGATGC AGATTTGGTT
AGCACTGTTC CAGAATCTGC TACGCCTGCT GCTCTTGCTT ACGCAGGAAA GTGTGGACTT
CCATATGTGG AGGTGCTGTG TAAAAACCGG TATGTAGGGA GAACCTTCAT TCAGCCAAAC
ATGAGGTTAA GACAACTTGG TGTTGCAAAA AAATTTGGAG TATTGTCAGA CAACTTTAAA
GGCAAAAGAA TTGTTCTTGT AGATGATTCA ATTGTCAGAG GCAATACCAT CTCACCTATA
ATAAAACTGC TCAAAGAATC TGGTGCAAAA GAGGTACACA TTCGAGTAGC TTCACCACCA
ATTAAATATC CATGCTTCAT GGGAATAAAC ATTCCTACAA AAGAAGAGCT CATTGCCAAT
AAACCAGAAT TTGATCACCT TGCAGAATAT CTAGGAGCAA ACAGTGTTGT GTATCTGTCA
GTAGAAGGAC TGGTTTCATC TGTACAAGAA GGGATAAAGT TTAAAAAACA GAAAGAGAAA
AAGCACGATA TTATGATCCA AGAAAATGGA AATGGTCTGG AATGTTTTGA AAAGAGTGGT
CATTGTACAG CTTGTCTCAC TGGAAAATAT CCTGTAGAAT TAGAATGGTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011532689.1
CDS180..1490
Misc Feature(1)207..1481(+)
Misc Feature(2)207..821(+)
Misc Feature(3)213..377(+)
Misc Feature(4)852..1262(+)
Misc Feature(5)909..1202(+)
Translation

Target ORF information:

RefSeq Version XM_011534387
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phosphoribosyl pyrophosphate amidotransferase (PPAT), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011534387

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGGAGTTC GGTGCCAACA TTCAAATCAC ACAAGGTATG CCACCACAGG AAAATGTGAA 
CTAGAAAATT GTCAGCCCTT CGTTGTTGAA ACACTTCATG GGAAGATAGC TGTGGCACAT
AATGGCGAAT TGGTAAATGC TGCTCGATTA AGGAAAAAGC TTCTGCGTCA TGGTATTGGT
CTGTCTACAA GTTCTGATAG TGAAATGATT ACCCAGTTAC TGGCGTATAC CCCTCCTCAG
GAACAAGATG ACACCCCAGA CTGGGTAGCC AGGATTAAAA ACTTGATGAA GGAAGCACCC
ACAGCATACT CCCTGCTTAT AATGCACAGA GATGTTATTT ATGCAGTACG AGATCCTTAT
GGAAATCGTC CCTTATGCAT TGGTCGTCTT ATTCCAGTGT CTGATATAAA TGACAAAGAG
AAAAAAACAT CAGAAACAGA AGGATGGGTG GTGTCTTCAG AATCTTGTAG CTTCTTATCT
ATTGGTGCAA GATATTACCG TGAAGTCTTG CCTGGAGAAA TTGTGGAAAT ATCCAGACAC
AATGTCCAAA CTCTTGATAT TATATCAAGG TCTGAAGGAA ACCCAGTGGC TTTTTGTATC
TTTGAATATG TTTATTTTGC AAGACCAGAC AGTATGTTCG AAGACCAAAT GGTTTATACA
GTAAGATACC GTTGTGGCCA GCAGCTAGCG ATTGAAGCAC CTGTGGATGC AGATTTGGTT
AGCACTGTTC CAGAATCTGC TACGCCTGCT GCTCTTGCTT ACGCAGGAAA GTGTGGACTT
CCATATGTGG AGGTGCTGTG TAAAAACCGG TATGTAGGGA GAACCTTCAT TCAGCCAAAC
ATGAGGTTAA GACAACTTGG TGTTGCAAAA AAATTTGGAG TATTGTCAGA CAACTTTAAA
GGCAAAAGAA TTGTTCTTGT AGATGATTCA ATTGTCAGAG GCAATACCAT CTCACCTATA
ATAAAACTGC TCAAAGAATC TGGTGCAAAA GAGGTACACA TTCGAGTAGC TTCACCACCA
ATTAAATATC CATGCTTCAT GGGAATAAAC ATTCCTACAA AAGAAGAGCT CATTGCCAAT
AAACCAGAAT TTGATCACCT TGCAGAATAT CTAGGAGCAA ACAGTGTTGT GTATCTGTCA
GTAGAAGGAC TGGTTTCATC TGTACAAGAA GGGATAAAGT TTAAAAAACA GAAAGAGAAA
AAGCACGATA TTATGATCCA AGAAAATGGA AATGGTCTGG AATGTTTTGA AAAGAGTGGT
CATTGTACAG CTTGTCTCAC TGGAAAATAT CCTGTAGAAT TAGAATGGTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu03961
Clone ID Related Accession (Same CDS sequence) NM_002703
Accession Version NM_002703.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1554bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product amidophosphoribosyltransferase proprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB039655.1, BC004200.2, AB209246.1 and U00238.1. On Mar 4, 2011 this sequence version replaced gi:29570797. ##Evidence-Data-START## Transcript exon combination :: U00238.1, D13757.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGAGCTGG AGGAGTTGGG GATCCGAGAG GAATGTGGCG TGTTCGGGTG CATCGCCTCA 
GGAGAGTGGC CCACGCAGCT GGATGTACCG CATGTGATCA CTCTGGGACT CGTGGGGCTG
CAGCACCGGG GTCAGGAGAG TGCTGGTATT GTGACTAGTG ATGGGAGTTC GGTGCCAACA
TTCAAATCAC ACAAGGGAAT GGGTCTTGTA AATCACGTCT TTACTGAAGA CAATTTGAAA
AAATTATATG TTTCAAATCT TGGAATTGGA CACACCAGGT ATGCCACCAC AGGAAAATGT
GAACTAGAAA ATTGTCAGCC CTTCGTTGTT GAAACACTTC ATGGGAAGAT AGCTGTGGCA
CATAATGGCG AATTGGTAAA TGCTGCTCGA TTAAGGAAAA AGCTTCTGCG TCATGGTATT
GGTCTGTCTA CAAGTTCTGA TAGTGAAATG ATTACCCAGT TACTGGCGTA TACCCCTCCT
CAGGAACAAG ATGACACCCC AGACTGGGTA GCCAGGATTA AAAACTTGAT GAAGGAAGCA
CCCACAGCAT ACTCCCTGCT TATAATGCAC AGAGATGTTA TTTATGCAGT ACGAGATCCT
TATGGAAATC GTCCCTTATG CATTGGTCGT CTTATTCCAG TGTCTGATAT AAATGACAAA
GAGAAAAAAA CATCAGAAAC AGAAGGATGG GTGGTGTCTT CAGAATCTTG TAGCTTCTTA
TCTATTGGTG CAAGATATTA CCGTGAAGTC TTGCCTGGAG AAATTGTGGA AATATCCAGA
CACAATGTCC AAACTCTTGA TATTATATCA AGGTCTGAAG GAAACCCAGT GGCTTTTTGT
ATCTTTGAAT ATGTTTATTT TGCAAGACCA GACAGTATGT TCGAAGACCA AATGGTTTAT
ACAGTAAGAT ACCGTTGTGG CCAGCAGCTA GCGATTGAAG CACCTGTGGA TGCAGATTTG
GTTAGCACTG TTCCAGAATC TGCTACGCCT GCTGCTCTTG CTTACGCAGG AAAGTGTGGA
CTTCCATATG TGGAGGTGCT GTGTAAAAAC CGGTATGTAG GGAGAACCTT CATTCAGCCA
AACATGAGGT TAAGACAACT TGGTGTTGCA AAAAAATTTG GAGTATTGTC AGACAACTTT
AAAGGCAAAA GAATTGTTCT TGTAGATGAT TCAATTGTCA GAGGCAATAC CATCTCACCT
ATAATAAAAC TGCTCAAAGA ATCTGGTGCA AAAGAGGTAC ACATTCGAGT AGCTTCACCA
CCAATTAAAT ATCCATGCTT CATGGGAATA AACATTCCTA CAAAAGAAGA GCTCATTGCC
AATAAACCAG AATTTGATCA CCTTGCAGAA TATCTAGGAG CAAACAGTGT TGTGTATCTG
TCAGTAGAAG GACTGGTTTC ATCTGTACAA GAAGGGATAA AGTTTAAAAA ACAGAAAGAG
AAAAAGCACG ATATTATGAT CCAAGAAAAT GGAAATGGTC TGGAATGTTT TGAAAAGAGT
GGTCATTGTA CAGCTTGTCT CACTGGAAAA TATCCTGTAG AATTAGAATG GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002694.3
CDS160..1713
Misc Feature(1)160..162(+)
Misc Feature(2)181..1704(+)
Misc Feature(3)193..1044(+)
Misc Feature(4)193..600(+)
Misc Feature(5)259..630(+)
Misc Feature(6)1075..1485(+)
Misc Feature(7)1132..1425(+)
Exon (1)1..287
Gene:PPAT
Gene Synonym:
Exon (2)288..354
Gene:PPAT
Gene Synonym:
Exon (3)355..561
Gene:PPAT
Gene Synonym:
Exon (4)562..674
Gene:PPAT
Gene Synonym:
Exon (5)675..820
Gene:PPAT
Gene Synonym:
Exon (6)821..893
Gene:PPAT
Gene Synonym:
Exon (7)894..1045
Gene:PPAT
Gene Synonym:
Exon (8)1046..1173
Gene:PPAT
Gene Synonym:
Exon (9)1174..1395
Gene:PPAT
Gene Synonym:
Exon (10)1396..1516
Gene:PPAT
Gene Synonym:
Exon (11)1517..3703
Gene:PPAT
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_002703
Organism Homo sapiens (human)
Definition Homo sapiens phosphoribosyl pyrophosphate amidotransferase (PPAT), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002703

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGAGCTGG AGGAGTTGGG GATCCGAGAG GAATGTGGCG TGTTCGGGTG CATCGCCTCA 
GGAGAGTGGC CCACGCAGCT GGATGTACCG CATGTGATCA CTCTGGGACT CGTGGGGCTG
CAGCACCGGG GTCAGGAGAG TGCTGGTATT GTGACTAGTG ATGGGAGTTC GGTGCCAACA
TTCAAATCAC ACAAGGGAAT GGGTCTTGTA AATCACGTCT TTACTGAAGA CAATTTGAAA
AAATTATATG TTTCAAATCT TGGAATTGGA CACACCAGGT ATGCCACCAC AGGAAAATGT
GAACTAGAAA ATTGTCAGCC CTTCGTTGTT GAAACACTTC ATGGGAAGAT AGCTGTGGCA
CATAATGGCG AATTGGTAAA TGCTGCTCGA TTAAGGAAAA AGCTTCTGCG TCATGGTATT
GGTCTGTCTA CAAGTTCTGA TAGTGAAATG ATTACCCAGT TACTGGCGTA TACCCCTCCT
CAGGAACAAG ATGACACCCC AGACTGGGTA GCCAGGATTA AAAACTTGAT GAAGGAAGCA
CCCACAGCAT ACTCCCTGCT TATAATGCAC AGAGATGTTA TTTATGCAGT ACGAGATCCT
TATGGAAATC GTCCCTTATG CATTGGTCGT CTTATTCCAG TGTCTGATAT AAATGACAAA
GAGAAAAAAA CATCAGAAAC AGAAGGATGG GTGGTGTCTT CAGAATCTTG TAGCTTCTTA
TCTATTGGTG CAAGATATTA CCGTGAAGTC TTGCCTGGAG AAATTGTGGA AATATCCAGA
CACAATGTCC AAACTCTTGA TATTATATCA AGGTCTGAAG GAAACCCAGT GGCTTTTTGT
ATCTTTGAAT ATGTTTATTT TGCAAGACCA GACAGTATGT TCGAAGACCA AATGGTTTAT
ACAGTAAGAT ACCGTTGTGG CCAGCAGCTA GCGATTGAAG CACCTGTGGA TGCAGATTTG
GTTAGCACTG TTCCAGAATC TGCTACGCCT GCTGCTCTTG CTTACGCAGG AAAGTGTGGA
CTTCCATATG TGGAGGTGCT GTGTAAAAAC CGGTATGTAG GGAGAACCTT CATTCAGCCA
AACATGAGGT TAAGACAACT TGGTGTTGCA AAAAAATTTG GAGTATTGTC AGACAACTTT
AAAGGCAAAA GAATTGTTCT TGTAGATGAT TCAATTGTCA GAGGCAATAC CATCTCACCT
ATAATAAAAC TGCTCAAAGA ATCTGGTGCA AAAGAGGTAC ACATTCGAGT AGCTTCACCA
CCAATTAAAT ATCCATGCTT CATGGGAATA AACATTCCTA CAAAAGAAGA GCTCATTGCC
AATAAACCAG AATTTGATCA CCTTGCAGAA TATCTAGGAG CAAACAGTGT TGTGTATCTG
TCAGTAGAAG GACTGGTTTC ATCTGTACAA GAAGGGATAA AGTTTAAAAA ACAGAAAGAG
AAAAAGCACG ATATTATGAT CCAAGAAAAT GGAAATGGTC TGGAATGTTT TGAAAAGAGT
GGTCATTGTA CAGCTTGTCT CACTGGAAAA TATCCTGTAG AATTAGAATG GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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