SPDL1 cDNA ORF clone, Homo sapiens(human)

The following SPDL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPDL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu68773 NM_001329640.2
Latest version!
Homo sapiens spindle apparatus coiled-coil protein 1 (SPDL1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu68773 NM_001329639.2
Latest version!
Homo sapiens spindle apparatus coiled-coil protein 1 (SPDL1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu68773 XM_011534587.2
Latest version!
Homo sapiens spindle apparatus coiled-coil protein 1 (SPDL1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu68773 XM_011534588.2
Latest version!
Homo sapiens spindle apparatus coiled-coil protein 1 (SPDL1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu68773 XM_011534589.2
Latest version!
Homo sapiens spindle apparatus coiled-coil protein 1 (SPDL1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu31741 NM_001329641.2
Latest version!
Homo sapiens spindle apparatus coiled-coil protein 1 (SPDL1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $265.30
$379.00
OHu31741 NM_017785.5
Latest version!
Homo sapiens spindle apparatus coiled-coil protein 1 (SPDL1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $265.30
$379.00
OHu68774 NM_001329642.2
Latest version!
Homo sapiens spindle apparatus coiled-coil protein 1 (SPDL1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu101265 NM_001329643.2
Latest version!
Homo sapiens spindle apparatus coiled-coil protein 1 (SPDL1), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OHu101265 NM_001329643.1 Homo sapiens spindle apparatus coiled-coil protein 1 (SPDL1), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OHu31741 NM_017785.4 Homo sapiens spindle apparatus coiled-coil protein 1 (SPDL1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $265.30
$379.00
OHu68773 NM_001329639.1 Homo sapiens spindle apparatus coiled-coil protein 1 (SPDL1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu68774 NM_001329642.1 Homo sapiens spindle apparatus coiled-coil protein 1 (SPDL1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu68773 NM_001329640.1 Homo sapiens spindle apparatus coiled-coil protein 1 (SPDL1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu31741 NM_001329641.1 Homo sapiens spindle apparatus coiled-coil protein 1 (SPDL1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $265.30
$379.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu68773
    Clone ID Related Accession (Same CDS sequence) XM_011534589.2 , XM_011534587.2 , NM_001329640.1 , NM_001329639.2 , NM_001329639.1 , NM_001329640.2 , XM_011534588.2
    Accession Version NM_001329640.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1869bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-24
    Organism Homo sapiens(human)
    Product protein Spindly isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008680.5 and BU616897.1. On Jul 21, 2016 this sequence version replaced XM_011534586.2. Transcript Variant: Variants 1 and 2 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.255751.1, SRR1660803.205705.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGAGGCAG ATATAATCAC AAATCTTCGA TGCAGGCTCA AAGAGGCTGA AGAAGAGCGA 
    CTAAAAGCTG CACAGTATGG TTTACAACTA GTAGAGAGTC AAAATGAATT ACAGAATCAA
    TTGGATAAAT GTCGTAATGA AATGATGACC ATGACTGAGA GTTATGAACA AGAAAAATAT
    ACCCTTCAAA GAGAAGTTGA ACTCAAGAGT CGAATGTTAG AAAGTTTGAG CTGCGAATGT
    GAAGCTATTA AACAACAACA AAAAATGCAC CTGGAGAAAT TGGAAGAACA ACTAAGCAGA
    AGCCATGGAC AGGAAGTGAA TGAACTAAAA ACTAAGATAG AAAAACTGAA AGTGGAATTA
    GATGAAGCCA GGCTTAGTGA AAAGCAGCTG AAGCACCAAG TAGATCATCA GAAGGAACTC
    CTCTCTTGTA AATCAGAGGA ACTGCGCGTA ATGTCTGAAC GTGTGCAGGA AAGCATGTCT
    TCAGAGATGC TGGCTCTTCA AATTGAGCTG ACAGAAATGG AGAGTATGAA GACCACCCTC
    AAAGAAGAAG TGAATGAACT ACAATACAGA CAAGAACAGC TAGAACTTCT TATTACTAAC
    CTAATGCGCC AGGTAGACCG GCTTAAAGAG GAAAAAGAGG AGCGAGAGAA AGAAGCAGTT
    TCTTACTATA ATGCCCTAGA GAAAGCTCGT GTAGCAAATC AAGATCTTCA GGTACAGTTG
    GACCAGGCAC TCCAGCAAGC CTTGGATCCC AATAGTAAAG GCAACTCTTT GTTTGCAGAG
    GTGGAAGATC GAAGGGCAGC AATGGAACGT CAGCTCATCA GTATGAAAGT CAAGTATCAG
    TCACTAAAGA AGCAAAATGT ATTTAACAGA GAACAGATGC AGAGAATGAA GTTACAAATT
    GCCACGTTGC TACAGATGAA AGGGTCTCAA ACTGAATTTG AGCAGCAGGA ACGGTTGCTT
    GCCATGTTGG AGCAGAAGAA TGGTGAAATA AAACATCTTT TAGGTGAAAT TAGAAATCTG
    GAGAAATTTA AGAATTTATA TGACAGTATG GAATCTAAGC CTTCAGTCGA CTCTGGTACT
    CTGGAAGATA ACACCTATTA TACAGATTTA CTTCAGATGA AGCTGGATAA CTTAAACAAA
    GAAATTGAAA GCACTAAAGG TGAATTGTCC ATACAGCGAA TGAAAGCATT ATTTGAGAGC
    CAGCGGGCTC TAGATATTGA GCGAAAACTT TTTGCAAATG AAAGATGCCT CCAGCTTTCA
    GAAAGTGAAA ATATGAAACT GAGAGCTAAA CTAGATGAAT TGAAACTAAA ATATGAACCT
    GAAGAGACAG TTGAAGTGCC TGTACTGAAA AAGAGGCGTG AGGTGCTCCC TGTGGATATA
    ACCACCGCTA AAGATGCATG TGTCAACAAC AGTGCTCTCG GGGGAGAAGT TTATCGATTA
    CCGCCTCAGA AAGAGGAGAC ACAGTCCTGC CCTAACAGTT TAGAAGATAA CAACTTGCAA
    TTAGAAAAAT CAGTTTCTAT ATACACACCA GTAGTCAGTC TCTCTCCTCA CAAAAATCTG
    CCCGTGGATA TGCAGCTGAA GAAGGAAAAG AAATGTGTGA AACTCATAGG AGTTCCCGCT
    GACGCTGAGG CCTTAAGTGA AAGAAGTGGA AACACCCCTA ACTCTCCCAG GTCAGTGTCC
    TCTTTTCCTC CAGGCAGCCA GCAGACCTCT CCATCTCTCC TCTCTCGCTG CATGAACTGT
    GCTGTCTGTT TCTTTATCTA CTTTCTTACA ATTGCATGCA GTATAATTCC TCAGTTTCAT
    CTACCTACCT TCAACTTTTC CAGAACTTTA AGAAAGACTA AACTGATTGC AAAGGGAAAG
    GACTCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001316569.1
    CDS111..1979
    Translation

    Target ORF information:

    RefSeq Version NM_001329640.1
    Organism Homo sapiens(human)
    Definition Homo sapiens spindle apparatus coiled-coil protein 1 (SPDL1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001329640.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGAGGCAG ATATAATCAC AAATCTTCGA TGCAGGCTCA AAGAGGCTGA AGAAGAGCGA 
    CTAAAAGCTG CACAGTATGG TTTACAACTA GTAGAGAGTC AAAATGAATT ACAGAATCAA
    TTGGATAAAT GTCGTAATGA AATGATGACC ATGACTGAGA GTTATGAACA AGAAAAATAT
    ACCCTTCAAA GAGAAGTTGA ACTCAAGAGT CGAATGTTAG AAAGTTTGAG CTGCGAATGT
    GAAGCTATTA AACAACAACA AAAAATGCAC CTGGAGAAAT TGGAAGAACA ACTAAGCAGA
    AGCCATGGAC AGGAAGTGAA TGAACTAAAA ACTAAGATAG AAAAACTGAA AGTGGAATTA
    GATGAAGCCA GGCTTAGTGA AAAGCAGCTG AAGCACCAAG TAGATCATCA GAAGGAACTC
    CTCTCTTGTA AATCAGAGGA ACTGCGCGTA ATGTCTGAAC GTGTGCAGGA AAGCATGTCT
    TCAGAGATGC TGGCTCTTCA AATTGAGCTG ACAGAAATGG AGAGTATGAA GACCACCCTC
    AAAGAAGAAG TGAATGAACT ACAATACAGA CAAGAACAGC TAGAACTTCT TATTACTAAC
    CTAATGCGCC AGGTAGACCG GCTTAAAGAG GAAAAAGAGG AGCGAGAGAA AGAAGCAGTT
    TCTTACTATA ATGCCCTAGA GAAAGCTCGT GTAGCAAATC AAGATCTTCA GGTACAGTTG
    GACCAGGCAC TCCAGCAAGC CTTGGATCCC AATAGTAAAG GCAACTCTTT GTTTGCAGAG
    GTGGAAGATC GAAGGGCAGC AATGGAACGT CAGCTCATCA GTATGAAAGT CAAGTATCAG
    TCACTAAAGA AGCAAAATGT ATTTAACAGA GAACAGATGC AGAGAATGAA GTTACAAATT
    GCCACGTTGC TACAGATGAA AGGGTCTCAA ACTGAATTTG AGCAGCAGGA ACGGTTGCTT
    GCCATGTTGG AGCAGAAGAA TGGTGAAATA AAACATCTTT TAGGTGAAAT TAGAAATCTG
    GAGAAATTTA AGAATTTATA TGACAGTATG GAATCTAAGC CTTCAGTCGA CTCTGGTACT
    CTGGAAGATA ACACCTATTA TACAGATTTA CTTCAGATGA AGCTGGATAA CTTAAACAAA
    GAAATTGAAA GCACTAAAGG TGAATTGTCC ATACAGCGAA TGAAAGCATT ATTTGAGAGC
    CAGCGGGCTC TAGATATTGA GCGAAAACTT TTTGCAAATG AAAGATGCCT CCAGCTTTCA
    GAAAGTGAAA ATATGAAACT GAGAGCTAAA CTAGATGAAT TGAAACTAAA ATATGAACCT
    GAAGAGACAG TTGAAGTGCC TGTACTGAAA AAGAGGCGTG AGGTGCTCCC TGTGGATATA
    ACCACCGCTA AAGATGCATG TGTCAACAAC AGTGCTCTCG GGGGAGAAGT TTATCGATTA
    CCGCCTCAGA AAGAGGAGAC ACAGTCCTGC CCTAACAGTT TAGAAGATAA CAACTTGCAA
    TTAGAAAAAT CAGTTTCTAT ATACACACCA GTAGTCAGTC TCTCTCCTCA CAAAAATCTG
    CCCGTGGATA TGCAGCTGAA GAAGGAAAAG AAATGTGTGA AACTCATAGG AGTTCCCGCT
    GACGCTGAGG CCTTAAGTGA AAGAAGTGGA AACACCCCTA ACTCTCCCAG GTCAGTGTCC
    TCTTTTCCTC CAGGCAGCCA GCAGACCTCT CCATCTCTCC TCTCTCGCTG CATGAACTGT
    GCTGTCTGTT TCTTTATCTA CTTTCTTACA ATTGCATGCA GTATAATTCC TCAGTTTCAT
    CTACCTACCT TCAACTTTTC CAGAACTTTA AGAAAGACTA AACTGATTGC AAAGGGAAAG
    GACTCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu68773
    Clone ID Related Accession (Same CDS sequence) XM_011534589.2 , XM_011534587.2 , NM_001329640.1 , NM_001329639.2 , NM_001329639.1 , NM_001329640.2 , XM_011534588.2
    Accession Version NM_001329639.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1869bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product protein Spindly isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008680.5 and BU616897.1. On May 31, 2019 this sequence version replaced NM_001329639.1. Transcript Variant: Variants 1 and 2 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.90559.1, SRR1803614.77703.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGAGGCAG ATATAATCAC AAATCTTCGA TGCAGGCTCA AAGAGGCTGA AGAAGAGCGA 
    CTAAAAGCTG CACAGTATGG TTTACAACTA GTAGAGAGTC AAAATGAATT ACAGAATCAA
    TTGGATAAAT GTCGTAATGA AATGATGACC ATGACTGAGA GTTATGAACA AGAAAAATAT
    ACCCTTCAAA GAGAAGTTGA ACTCAAGAGT CGAATGTTAG AAAGTTTGAG CTGCGAATGT
    GAAGCTATTA AACAACAACA AAAAATGCAC CTGGAGAAAT TGGAAGAACA ACTAAGCAGA
    AGCCATGGAC AGGAAGTGAA TGAACTAAAA ACTAAGATAG AAAAACTGAA AGTGGAATTA
    GATGAAGCCA GGCTTAGTGA AAAGCAGCTG AAGCACCAAG TAGATCATCA GAAGGAACTC
    CTCTCTTGTA AATCAGAGGA ACTGCGCGTA ATGTCTGAAC GTGTGCAGGA AAGCATGTCT
    TCAGAGATGC TGGCTCTTCA AATTGAGCTG ACAGAAATGG AGAGTATGAA GACCACCCTC
    AAAGAAGAAG TGAATGAACT ACAATACAGA CAAGAACAGC TAGAACTTCT TATTACTAAC
    CTAATGCGCC AGGTAGACCG GCTTAAAGAG GAAAAAGAGG AGCGAGAGAA AGAAGCAGTT
    TCTTACTATA ATGCCCTAGA GAAAGCTCGT GTAGCAAATC AAGATCTTCA GGTACAGTTG
    GACCAGGCAC TCCAGCAAGC CTTGGATCCC AATAGTAAAG GCAACTCTTT GTTTGCAGAG
    GTGGAAGATC GAAGGGCAGC AATGGAACGT CAGCTCATCA GTATGAAAGT CAAGTATCAG
    TCACTAAAGA AGCAAAATGT ATTTAACAGA GAACAGATGC AGAGAATGAA GTTACAAATT
    GCCACGTTGC TACAGATGAA AGGGTCTCAA ACTGAATTTG AGCAGCAGGA ACGGTTGCTT
    GCCATGTTGG AGCAGAAGAA TGGTGAAATA AAACATCTTT TAGGTGAAAT TAGAAATCTG
    GAGAAATTTA AGAATTTATA TGACAGTATG GAATCTAAGC CTTCAGTCGA CTCTGGTACT
    CTGGAAGATA ACACCTATTA TACAGATTTA CTTCAGATGA AGCTGGATAA CTTAAACAAA
    GAAATTGAAA GCACTAAAGG TGAATTGTCC ATACAGCGAA TGAAAGCATT ATTTGAGAGC
    CAGCGGGCTC TAGATATTGA GCGAAAACTT TTTGCAAATG AAAGATGCCT CCAGCTTTCA
    GAAAGTGAAA ATATGAAACT GAGAGCTAAA CTAGATGAAT TGAAACTAAA ATATGAACCT
    GAAGAGACAG TTGAAGTGCC TGTACTGAAA AAGAGGCGTG AGGTGCTCCC TGTGGATATA
    ACCACCGCTA AAGATGCATG TGTCAACAAC AGTGCTCTCG GGGGAGAAGT TTATCGATTA
    CCGCCTCAGA AAGAGGAGAC ACAGTCCTGC CCTAACAGTT TAGAAGATAA CAACTTGCAA
    TTAGAAAAAT CAGTTTCTAT ATACACACCA GTAGTCAGTC TCTCTCCTCA CAAAAATCTG
    CCCGTGGATA TGCAGCTGAA GAAGGAAAAG AAATGTGTGA AACTCATAGG AGTTCCCGCT
    GACGCTGAGG CCTTAAGTGA AAGAAGTGGA AACACCCCTA ACTCTCCCAG GTCAGTGTCC
    TCTTTTCCTC CAGGCAGCCA GCAGACCTCT CCATCTCTCC TCTCTCGCTG CATGAACTGT
    GCTGTCTGTT TCTTTATCTA CTTTCTTACA ATTGCATGCA GTATAATTCC TCAGTTTCAT
    CTACCTACCT TCAACTTTTC CAGAACTTTA AGAAAGACTA AACTGATTGC AAAGGGAAAG
    GACTCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001316568.1
    CDS141..2009
    Translation

    Target ORF information:

    RefSeq Version NM_001329639.2
    Organism Homo sapiens(human)
    Definition Homo sapiens spindle apparatus coiled-coil protein 1 (SPDL1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001329639.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGAGGCAG ATATAATCAC AAATCTTCGA TGCAGGCTCA AAGAGGCTGA AGAAGAGCGA 
    CTAAAAGCTG CACAGTATGG TTTACAACTA GTAGAGAGTC AAAATGAATT ACAGAATCAA
    TTGGATAAAT GTCGTAATGA AATGATGACC ATGACTGAGA GTTATGAACA AGAAAAATAT
    ACCCTTCAAA GAGAAGTTGA ACTCAAGAGT CGAATGTTAG AAAGTTTGAG CTGCGAATGT
    GAAGCTATTA AACAACAACA AAAAATGCAC CTGGAGAAAT TGGAAGAACA ACTAAGCAGA
    AGCCATGGAC AGGAAGTGAA TGAACTAAAA ACTAAGATAG AAAAACTGAA AGTGGAATTA
    GATGAAGCCA GGCTTAGTGA AAAGCAGCTG AAGCACCAAG TAGATCATCA GAAGGAACTC
    CTCTCTTGTA AATCAGAGGA ACTGCGCGTA ATGTCTGAAC GTGTGCAGGA AAGCATGTCT
    TCAGAGATGC TGGCTCTTCA AATTGAGCTG ACAGAAATGG AGAGTATGAA GACCACCCTC
    AAAGAAGAAG TGAATGAACT ACAATACAGA CAAGAACAGC TAGAACTTCT TATTACTAAC
    CTAATGCGCC AGGTAGACCG GCTTAAAGAG GAAAAAGAGG AGCGAGAGAA AGAAGCAGTT
    TCTTACTATA ATGCCCTAGA GAAAGCTCGT GTAGCAAATC AAGATCTTCA GGTACAGTTG
    GACCAGGCAC TCCAGCAAGC CTTGGATCCC AATAGTAAAG GCAACTCTTT GTTTGCAGAG
    GTGGAAGATC GAAGGGCAGC AATGGAACGT CAGCTCATCA GTATGAAAGT CAAGTATCAG
    TCACTAAAGA AGCAAAATGT ATTTAACAGA GAACAGATGC AGAGAATGAA GTTACAAATT
    GCCACGTTGC TACAGATGAA AGGGTCTCAA ACTGAATTTG AGCAGCAGGA ACGGTTGCTT
    GCCATGTTGG AGCAGAAGAA TGGTGAAATA AAACATCTTT TAGGTGAAAT TAGAAATCTG
    GAGAAATTTA AGAATTTATA TGACAGTATG GAATCTAAGC CTTCAGTCGA CTCTGGTACT
    CTGGAAGATA ACACCTATTA TACAGATTTA CTTCAGATGA AGCTGGATAA CTTAAACAAA
    GAAATTGAAA GCACTAAAGG TGAATTGTCC ATACAGCGAA TGAAAGCATT ATTTGAGAGC
    CAGCGGGCTC TAGATATTGA GCGAAAACTT TTTGCAAATG AAAGATGCCT CCAGCTTTCA
    GAAAGTGAAA ATATGAAACT GAGAGCTAAA CTAGATGAAT TGAAACTAAA ATATGAACCT
    GAAGAGACAG TTGAAGTGCC TGTACTGAAA AAGAGGCGTG AGGTGCTCCC TGTGGATATA
    ACCACCGCTA AAGATGCATG TGTCAACAAC AGTGCTCTCG GGGGAGAAGT TTATCGATTA
    CCGCCTCAGA AAGAGGAGAC ACAGTCCTGC CCTAACAGTT TAGAAGATAA CAACTTGCAA
    TTAGAAAAAT CAGTTTCTAT ATACACACCA GTAGTCAGTC TCTCTCCTCA CAAAAATCTG
    CCCGTGGATA TGCAGCTGAA GAAGGAAAAG AAATGTGTGA AACTCATAGG AGTTCCCGCT
    GACGCTGAGG CCTTAAGTGA AAGAAGTGGA AACACCCCTA ACTCTCCCAG GTCAGTGTCC
    TCTTTTCCTC CAGGCAGCCA GCAGACCTCT CCATCTCTCC TCTCTCGCTG CATGAACTGT
    GCTGTCTGTT TCTTTATCTA CTTTCTTACA ATTGCATGCA GTATAATTCC TCAGTTTCAT
    CTACCTACCT TCAACTTTTC CAGAACTTTA AGAAAGACTA AACTGATTGC AAAGGGAAAG
    GACTCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu68773
    Clone ID Related Accession (Same CDS sequence) XM_011534589.2 , XM_011534587.2 , NM_001329640.1 , NM_001329639.2 , NM_001329639.1 , NM_001329640.2 , XM_011534588.2
    Accession Version NM_001329639.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1869bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-25
    Organism Homo sapiens(human)
    Product protein Spindly isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008680.5 and BU616897.1. On Jul 21, 2016 this sequence version replaced XM_011534585.1. Transcript Variant: Variants 1 and 2 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.153717.1, SRR1803614.171625.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGAGGCAG ATATAATCAC AAATCTTCGA TGCAGGCTCA AAGAGGCTGA AGAAGAGCGA 
    CTAAAAGCTG CACAGTATGG TTTACAACTA GTAGAGAGTC AAAATGAATT ACAGAATCAA
    TTGGATAAAT GTCGTAATGA AATGATGACC ATGACTGAGA GTTATGAACA AGAAAAATAT
    ACCCTTCAAA GAGAAGTTGA ACTCAAGAGT CGAATGTTAG AAAGTTTGAG CTGCGAATGT
    GAAGCTATTA AACAACAACA AAAAATGCAC CTGGAGAAAT TGGAAGAACA ACTAAGCAGA
    AGCCATGGAC AGGAAGTGAA TGAACTAAAA ACTAAGATAG AAAAACTGAA AGTGGAATTA
    GATGAAGCCA GGCTTAGTGA AAAGCAGCTG AAGCACCAAG TAGATCATCA GAAGGAACTC
    CTCTCTTGTA AATCAGAGGA ACTGCGCGTA ATGTCTGAAC GTGTGCAGGA AAGCATGTCT
    TCAGAGATGC TGGCTCTTCA AATTGAGCTG ACAGAAATGG AGAGTATGAA GACCACCCTC
    AAAGAAGAAG TGAATGAACT ACAATACAGA CAAGAACAGC TAGAACTTCT TATTACTAAC
    CTAATGCGCC AGGTAGACCG GCTTAAAGAG GAAAAAGAGG AGCGAGAGAA AGAAGCAGTT
    TCTTACTATA ATGCCCTAGA GAAAGCTCGT GTAGCAAATC AAGATCTTCA GGTACAGTTG
    GACCAGGCAC TCCAGCAAGC CTTGGATCCC AATAGTAAAG GCAACTCTTT GTTTGCAGAG
    GTGGAAGATC GAAGGGCAGC AATGGAACGT CAGCTCATCA GTATGAAAGT CAAGTATCAG
    TCACTAAAGA AGCAAAATGT ATTTAACAGA GAACAGATGC AGAGAATGAA GTTACAAATT
    GCCACGTTGC TACAGATGAA AGGGTCTCAA ACTGAATTTG AGCAGCAGGA ACGGTTGCTT
    GCCATGTTGG AGCAGAAGAA TGGTGAAATA AAACATCTTT TAGGTGAAAT TAGAAATCTG
    GAGAAATTTA AGAATTTATA TGACAGTATG GAATCTAAGC CTTCAGTCGA CTCTGGTACT
    CTGGAAGATA ACACCTATTA TACAGATTTA CTTCAGATGA AGCTGGATAA CTTAAACAAA
    GAAATTGAAA GCACTAAAGG TGAATTGTCC ATACAGCGAA TGAAAGCATT ATTTGAGAGC
    CAGCGGGCTC TAGATATTGA GCGAAAACTT TTTGCAAATG AAAGATGCCT CCAGCTTTCA
    GAAAGTGAAA ATATGAAACT GAGAGCTAAA CTAGATGAAT TGAAACTAAA ATATGAACCT
    GAAGAGACAG TTGAAGTGCC TGTACTGAAA AAGAGGCGTG AGGTGCTCCC TGTGGATATA
    ACCACCGCTA AAGATGCATG TGTCAACAAC AGTGCTCTCG GGGGAGAAGT TTATCGATTA
    CCGCCTCAGA AAGAGGAGAC ACAGTCCTGC CCTAACAGTT TAGAAGATAA CAACTTGCAA
    TTAGAAAAAT CAGTTTCTAT ATACACACCA GTAGTCAGTC TCTCTCCTCA CAAAAATCTG
    CCCGTGGATA TGCAGCTGAA GAAGGAAAAG AAATGTGTGA AACTCATAGG AGTTCCCGCT
    GACGCTGAGG CCTTAAGTGA AAGAAGTGGA AACACCCCTA ACTCTCCCAG GTCAGTGTCC
    TCTTTTCCTC CAGGCAGCCA GCAGACCTCT CCATCTCTCC TCTCTCGCTG CATGAACTGT
    GCTGTCTGTT TCTTTATCTA CTTTCTTACA ATTGCATGCA GTATAATTCC TCAGTTTCAT
    CTACCTACCT TCAACTTTTC CAGAACTTTA AGAAAGACTA AACTGATTGC AAAGGGAAAG
    GACTCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001316568.1
    CDS280..2148
    Translation

    Target ORF information:

    RefSeq Version NM_001329639.1
    Organism Homo sapiens(human)
    Definition Homo sapiens spindle apparatus coiled-coil protein 1 (SPDL1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001329639.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGAGGCAG ATATAATCAC AAATCTTCGA TGCAGGCTCA AAGAGGCTGA AGAAGAGCGA 
    CTAAAAGCTG CACAGTATGG TTTACAACTA GTAGAGAGTC AAAATGAATT ACAGAATCAA
    TTGGATAAAT GTCGTAATGA AATGATGACC ATGACTGAGA GTTATGAACA AGAAAAATAT
    ACCCTTCAAA GAGAAGTTGA ACTCAAGAGT CGAATGTTAG AAAGTTTGAG CTGCGAATGT
    GAAGCTATTA AACAACAACA AAAAATGCAC CTGGAGAAAT TGGAAGAACA ACTAAGCAGA
    AGCCATGGAC AGGAAGTGAA TGAACTAAAA ACTAAGATAG AAAAACTGAA AGTGGAATTA
    GATGAAGCCA GGCTTAGTGA AAAGCAGCTG AAGCACCAAG TAGATCATCA GAAGGAACTC
    CTCTCTTGTA AATCAGAGGA ACTGCGCGTA ATGTCTGAAC GTGTGCAGGA AAGCATGTCT
    TCAGAGATGC TGGCTCTTCA AATTGAGCTG ACAGAAATGG AGAGTATGAA GACCACCCTC
    AAAGAAGAAG TGAATGAACT ACAATACAGA CAAGAACAGC TAGAACTTCT TATTACTAAC
    CTAATGCGCC AGGTAGACCG GCTTAAAGAG GAAAAAGAGG AGCGAGAGAA AGAAGCAGTT
    TCTTACTATA ATGCCCTAGA GAAAGCTCGT GTAGCAAATC AAGATCTTCA GGTACAGTTG
    GACCAGGCAC TCCAGCAAGC CTTGGATCCC AATAGTAAAG GCAACTCTTT GTTTGCAGAG
    GTGGAAGATC GAAGGGCAGC AATGGAACGT CAGCTCATCA GTATGAAAGT CAAGTATCAG
    TCACTAAAGA AGCAAAATGT ATTTAACAGA GAACAGATGC AGAGAATGAA GTTACAAATT
    GCCACGTTGC TACAGATGAA AGGGTCTCAA ACTGAATTTG AGCAGCAGGA ACGGTTGCTT
    GCCATGTTGG AGCAGAAGAA TGGTGAAATA AAACATCTTT TAGGTGAAAT TAGAAATCTG
    GAGAAATTTA AGAATTTATA TGACAGTATG GAATCTAAGC CTTCAGTCGA CTCTGGTACT
    CTGGAAGATA ACACCTATTA TACAGATTTA CTTCAGATGA AGCTGGATAA CTTAAACAAA
    GAAATTGAAA GCACTAAAGG TGAATTGTCC ATACAGCGAA TGAAAGCATT ATTTGAGAGC
    CAGCGGGCTC TAGATATTGA GCGAAAACTT TTTGCAAATG AAAGATGCCT CCAGCTTTCA
    GAAAGTGAAA ATATGAAACT GAGAGCTAAA CTAGATGAAT TGAAACTAAA ATATGAACCT
    GAAGAGACAG TTGAAGTGCC TGTACTGAAA AAGAGGCGTG AGGTGCTCCC TGTGGATATA
    ACCACCGCTA AAGATGCATG TGTCAACAAC AGTGCTCTCG GGGGAGAAGT TTATCGATTA
    CCGCCTCAGA AAGAGGAGAC ACAGTCCTGC CCTAACAGTT TAGAAGATAA CAACTTGCAA
    TTAGAAAAAT CAGTTTCTAT ATACACACCA GTAGTCAGTC TCTCTCCTCA CAAAAATCTG
    CCCGTGGATA TGCAGCTGAA GAAGGAAAAG AAATGTGTGA AACTCATAGG AGTTCCCGCT
    GACGCTGAGG CCTTAAGTGA AAGAAGTGGA AACACCCCTA ACTCTCCCAG GTCAGTGTCC
    TCTTTTCCTC CAGGCAGCCA GCAGACCTCT CCATCTCTCC TCTCTCGCTG CATGAACTGT
    GCTGTCTGTT TCTTTATCTA CTTTCTTACA ATTGCATGCA GTATAATTCC TCAGTTTCAT
    CTACCTACCT TCAACTTTTC CAGAACTTTA AGAAAGACTA AACTGATTGC AAAGGGAAAG
    GACTCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu68773
    Clone ID Related Accession (Same CDS sequence) XM_011534589.2 , XM_011534587.2 , NM_001329640.1 , NM_001329639.2 , NM_001329639.1 , NM_001329640.2 , XM_011534588.2
    Accession Version NM_001329640.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1869bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product protein Spindly isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008680.5 and BU616897.1. On May 31, 2019 this sequence version replaced NM_001329640.1. Transcript Variant: Variants 1 and 2 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.181901.1, SRR1660809.255751.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGAGGCAG ATATAATCAC AAATCTTCGA TGCAGGCTCA AAGAGGCTGA AGAAGAGCGA 
    CTAAAAGCTG CACAGTATGG TTTACAACTA GTAGAGAGTC AAAATGAATT ACAGAATCAA
    TTGGATAAAT GTCGTAATGA AATGATGACC ATGACTGAGA GTTATGAACA AGAAAAATAT
    ACCCTTCAAA GAGAAGTTGA ACTCAAGAGT CGAATGTTAG AAAGTTTGAG CTGCGAATGT
    GAAGCTATTA AACAACAACA AAAAATGCAC CTGGAGAAAT TGGAAGAACA ACTAAGCAGA
    AGCCATGGAC AGGAAGTGAA TGAACTAAAA ACTAAGATAG AAAAACTGAA AGTGGAATTA
    GATGAAGCCA GGCTTAGTGA AAAGCAGCTG AAGCACCAAG TAGATCATCA GAAGGAACTC
    CTCTCTTGTA AATCAGAGGA ACTGCGCGTA ATGTCTGAAC GTGTGCAGGA AAGCATGTCT
    TCAGAGATGC TGGCTCTTCA AATTGAGCTG ACAGAAATGG AGAGTATGAA GACCACCCTC
    AAAGAAGAAG TGAATGAACT ACAATACAGA CAAGAACAGC TAGAACTTCT TATTACTAAC
    CTAATGCGCC AGGTAGACCG GCTTAAAGAG GAAAAAGAGG AGCGAGAGAA AGAAGCAGTT
    TCTTACTATA ATGCCCTAGA GAAAGCTCGT GTAGCAAATC AAGATCTTCA GGTACAGTTG
    GACCAGGCAC TCCAGCAAGC CTTGGATCCC AATAGTAAAG GCAACTCTTT GTTTGCAGAG
    GTGGAAGATC GAAGGGCAGC AATGGAACGT CAGCTCATCA GTATGAAAGT CAAGTATCAG
    TCACTAAAGA AGCAAAATGT ATTTAACAGA GAACAGATGC AGAGAATGAA GTTACAAATT
    GCCACGTTGC TACAGATGAA AGGGTCTCAA ACTGAATTTG AGCAGCAGGA ACGGTTGCTT
    GCCATGTTGG AGCAGAAGAA TGGTGAAATA AAACATCTTT TAGGTGAAAT TAGAAATCTG
    GAGAAATTTA AGAATTTATA TGACAGTATG GAATCTAAGC CTTCAGTCGA CTCTGGTACT
    CTGGAAGATA ACACCTATTA TACAGATTTA CTTCAGATGA AGCTGGATAA CTTAAACAAA
    GAAATTGAAA GCACTAAAGG TGAATTGTCC ATACAGCGAA TGAAAGCATT ATTTGAGAGC
    CAGCGGGCTC TAGATATTGA GCGAAAACTT TTTGCAAATG AAAGATGCCT CCAGCTTTCA
    GAAAGTGAAA ATATGAAACT GAGAGCTAAA CTAGATGAAT TGAAACTAAA ATATGAACCT
    GAAGAGACAG TTGAAGTGCC TGTACTGAAA AAGAGGCGTG AGGTGCTCCC TGTGGATATA
    ACCACCGCTA AAGATGCATG TGTCAACAAC AGTGCTCTCG GGGGAGAAGT TTATCGATTA
    CCGCCTCAGA AAGAGGAGAC ACAGTCCTGC CCTAACAGTT TAGAAGATAA CAACTTGCAA
    TTAGAAAAAT CAGTTTCTAT ATACACACCA GTAGTCAGTC TCTCTCCTCA CAAAAATCTG
    CCCGTGGATA TGCAGCTGAA GAAGGAAAAG AAATGTGTGA AACTCATAGG AGTTCCCGCT
    GACGCTGAGG CCTTAAGTGA AAGAAGTGGA AACACCCCTA ACTCTCCCAG GTCAGTGTCC
    TCTTTTCCTC CAGGCAGCCA GCAGACCTCT CCATCTCTCC TCTCTCGCTG CATGAACTGT
    GCTGTCTGTT TCTTTATCTA CTTTCTTACA ATTGCATGCA GTATAATTCC TCAGTTTCAT
    CTACCTACCT TCAACTTTTC CAGAACTTTA AGAAAGACTA AACTGATTGC AAAGGGAAAG
    GACTCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001316569.1
    CDS111..1979
    Translation

    Target ORF information:

    RefSeq Version NM_001329640.2
    Organism Homo sapiens(human)
    Definition Homo sapiens spindle apparatus coiled-coil protein 1 (SPDL1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001329640.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGAGGCAG ATATAATCAC AAATCTTCGA TGCAGGCTCA AAGAGGCTGA AGAAGAGCGA 
    CTAAAAGCTG CACAGTATGG TTTACAACTA GTAGAGAGTC AAAATGAATT ACAGAATCAA
    TTGGATAAAT GTCGTAATGA AATGATGACC ATGACTGAGA GTTATGAACA AGAAAAATAT
    ACCCTTCAAA GAGAAGTTGA ACTCAAGAGT CGAATGTTAG AAAGTTTGAG CTGCGAATGT
    GAAGCTATTA AACAACAACA AAAAATGCAC CTGGAGAAAT TGGAAGAACA ACTAAGCAGA
    AGCCATGGAC AGGAAGTGAA TGAACTAAAA ACTAAGATAG AAAAACTGAA AGTGGAATTA
    GATGAAGCCA GGCTTAGTGA AAAGCAGCTG AAGCACCAAG TAGATCATCA GAAGGAACTC
    CTCTCTTGTA AATCAGAGGA ACTGCGCGTA ATGTCTGAAC GTGTGCAGGA AAGCATGTCT
    TCAGAGATGC TGGCTCTTCA AATTGAGCTG ACAGAAATGG AGAGTATGAA GACCACCCTC
    AAAGAAGAAG TGAATGAACT ACAATACAGA CAAGAACAGC TAGAACTTCT TATTACTAAC
    CTAATGCGCC AGGTAGACCG GCTTAAAGAG GAAAAAGAGG AGCGAGAGAA AGAAGCAGTT
    TCTTACTATA ATGCCCTAGA GAAAGCTCGT GTAGCAAATC AAGATCTTCA GGTACAGTTG
    GACCAGGCAC TCCAGCAAGC CTTGGATCCC AATAGTAAAG GCAACTCTTT GTTTGCAGAG
    GTGGAAGATC GAAGGGCAGC AATGGAACGT CAGCTCATCA GTATGAAAGT CAAGTATCAG
    TCACTAAAGA AGCAAAATGT ATTTAACAGA GAACAGATGC AGAGAATGAA GTTACAAATT
    GCCACGTTGC TACAGATGAA AGGGTCTCAA ACTGAATTTG AGCAGCAGGA ACGGTTGCTT
    GCCATGTTGG AGCAGAAGAA TGGTGAAATA AAACATCTTT TAGGTGAAAT TAGAAATCTG
    GAGAAATTTA AGAATTTATA TGACAGTATG GAATCTAAGC CTTCAGTCGA CTCTGGTACT
    CTGGAAGATA ACACCTATTA TACAGATTTA CTTCAGATGA AGCTGGATAA CTTAAACAAA
    GAAATTGAAA GCACTAAAGG TGAATTGTCC ATACAGCGAA TGAAAGCATT ATTTGAGAGC
    CAGCGGGCTC TAGATATTGA GCGAAAACTT TTTGCAAATG AAAGATGCCT CCAGCTTTCA
    GAAAGTGAAA ATATGAAACT GAGAGCTAAA CTAGATGAAT TGAAACTAAA ATATGAACCT
    GAAGAGACAG TTGAAGTGCC TGTACTGAAA AAGAGGCGTG AGGTGCTCCC TGTGGATATA
    ACCACCGCTA AAGATGCATG TGTCAACAAC AGTGCTCTCG GGGGAGAAGT TTATCGATTA
    CCGCCTCAGA AAGAGGAGAC ACAGTCCTGC CCTAACAGTT TAGAAGATAA CAACTTGCAA
    TTAGAAAAAT CAGTTTCTAT ATACACACCA GTAGTCAGTC TCTCTCCTCA CAAAAATCTG
    CCCGTGGATA TGCAGCTGAA GAAGGAAAAG AAATGTGTGA AACTCATAGG AGTTCCCGCT
    GACGCTGAGG CCTTAAGTGA AAGAAGTGGA AACACCCCTA ACTCTCCCAG GTCAGTGTCC
    TCTTTTCCTC CAGGCAGCCA GCAGACCTCT CCATCTCTCC TCTCTCGCTG CATGAACTGT
    GCTGTCTGTT TCTTTATCTA CTTTCTTACA ATTGCATGCA GTATAATTCC TCAGTTTCAT
    CTACCTACCT TCAACTTTTC CAGAACTTTA AGAAAGACTA AACTGATTGC AAAGGGAAAG
    GACTCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu68773
    Clone ID Related Accession (Same CDS sequence) XM_011534589.2 , XM_011534587.2 , NM_001329640.1 , NM_001329639.2 , NM_001329639.1 , NM_001329640.2 , XM_011534588.2
    Accession Version XM_011534589.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1869bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein Spindly isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011534589.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGAGGCAG ATATAATCAC AAATCTTCGA TGCAGGCTCA AAGAGGCTGA AGAAGAGCGA 
    CTAAAAGCTG CACAGTATGG TTTACAACTA GTAGAGAGTC AAAATGAATT ACAGAATCAA
    TTGGATAAAT GTCGTAATGA AATGATGACC ATGACTGAGA GTTATGAACA AGAAAAATAT
    ACCCTTCAAA GAGAAGTTGA ACTCAAGAGT CGAATGTTAG AAAGTTTGAG CTGCGAATGT
    GAAGCTATTA AACAACAACA AAAAATGCAC CTGGAGAAAT TGGAAGAACA ACTAAGCAGA
    AGCCATGGAC AGGAAGTGAA TGAACTAAAA ACTAAGATAG AAAAACTGAA AGTGGAATTA
    GATGAAGCCA GGCTTAGTGA AAAGCAGCTG AAGCACCAAG TAGATCATCA GAAGGAACTC
    CTCTCTTGTA AATCAGAGGA ACTGCGCGTA ATGTCTGAAC GTGTGCAGGA AAGCATGTCT
    TCAGAGATGC TGGCTCTTCA AATTGAGCTG ACAGAAATGG AGAGTATGAA GACCACCCTC
    AAAGAAGAAG TGAATGAACT ACAATACAGA CAAGAACAGC TAGAACTTCT TATTACTAAC
    CTAATGCGCC AGGTAGACCG GCTTAAAGAG GAAAAAGAGG AGCGAGAGAA AGAAGCAGTT
    TCTTACTATA ATGCCCTAGA GAAAGCTCGT GTAGCAAATC AAGATCTTCA GGTACAGTTG
    GACCAGGCAC TCCAGCAAGC CTTGGATCCC AATAGTAAAG GCAACTCTTT GTTTGCAGAG
    GTGGAAGATC GAAGGGCAGC AATGGAACGT CAGCTCATCA GTATGAAAGT CAAGTATCAG
    TCACTAAAGA AGCAAAATGT ATTTAACAGA GAACAGATGC AGAGAATGAA GTTACAAATT
    GCCACGTTGC TACAGATGAA AGGGTCTCAA ACTGAATTTG AGCAGCAGGA ACGGTTGCTT
    GCCATGTTGG AGCAGAAGAA TGGTGAAATA AAACATCTTT TAGGTGAAAT TAGAAATCTG
    GAGAAATTTA AGAATTTATA TGACAGTATG GAATCTAAGC CTTCAGTCGA CTCTGGTACT
    CTGGAAGATA ACACCTATTA TACAGATTTA CTTCAGATGA AGCTGGATAA CTTAAACAAA
    GAAATTGAAA GCACTAAAGG TGAATTGTCC ATACAGCGAA TGAAAGCATT ATTTGAGAGC
    CAGCGGGCTC TAGATATTGA GCGAAAACTT TTTGCAAATG AAAGATGCCT CCAGCTTTCA
    GAAAGTGAAA ATATGAAACT GAGAGCTAAA CTAGATGAAT TGAAACTAAA ATATGAACCT
    GAAGAGACAG TTGAAGTGCC TGTACTGAAA AAGAGGCGTG AGGTGCTCCC TGTGGATATA
    ACCACCGCTA AAGATGCATG TGTCAACAAC AGTGCTCTCG GGGGAGAAGT TTATCGATTA
    CCGCCTCAGA AAGAGGAGAC ACAGTCCTGC CCTAACAGTT TAGAAGATAA CAACTTGCAA
    TTAGAAAAAT CAGTTTCTAT ATACACACCA GTAGTCAGTC TCTCTCCTCA CAAAAATCTG
    CCCGTGGATA TGCAGCTGAA GAAGGAAAAG AAATGTGTGA AACTCATAGG AGTTCCCGCT
    GACGCTGAGG CCTTAAGTGA AAGAAGTGGA AACACCCCTA ACTCTCCCAG GTCAGTGTCC
    TCTTTTCCTC CAGGCAGCCA GCAGACCTCT CCATCTCTCC TCTCTCGCTG CATGAACTGT
    GCTGTCTGTT TCTTTATCTA CTTTCTTACA ATTGCATGCA GTATAATTCC TCAGTTTCAT
    CTACCTACCT TCAACTTTTC CAGAACTTTA AGAAAGACTA AACTGATTGC AAAGGGAAAG
    GACTCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011532891.1
    CDS175..2043
    Translation

    Target ORF information:

    RefSeq Version XM_011534589.2
    Organism Homo sapiens(human)
    Definition Homo sapiens spindle apparatus coiled-coil protein 1 (SPDL1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011534589.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGAGGCAG ATATAATCAC AAATCTTCGA TGCAGGCTCA AAGAGGCTGA AGAAGAGCGA 
    CTAAAAGCTG CACAGTATGG TTTACAACTA GTAGAGAGTC AAAATGAATT ACAGAATCAA
    TTGGATAAAT GTCGTAATGA AATGATGACC ATGACTGAGA GTTATGAACA AGAAAAATAT
    ACCCTTCAAA GAGAAGTTGA ACTCAAGAGT CGAATGTTAG AAAGTTTGAG CTGCGAATGT
    GAAGCTATTA AACAACAACA AAAAATGCAC CTGGAGAAAT TGGAAGAACA ACTAAGCAGA
    AGCCATGGAC AGGAAGTGAA TGAACTAAAA ACTAAGATAG AAAAACTGAA AGTGGAATTA
    GATGAAGCCA GGCTTAGTGA AAAGCAGCTG AAGCACCAAG TAGATCATCA GAAGGAACTC
    CTCTCTTGTA AATCAGAGGA ACTGCGCGTA ATGTCTGAAC GTGTGCAGGA AAGCATGTCT
    TCAGAGATGC TGGCTCTTCA AATTGAGCTG ACAGAAATGG AGAGTATGAA GACCACCCTC
    AAAGAAGAAG TGAATGAACT ACAATACAGA CAAGAACAGC TAGAACTTCT TATTACTAAC
    CTAATGCGCC AGGTAGACCG GCTTAAAGAG GAAAAAGAGG AGCGAGAGAA AGAAGCAGTT
    TCTTACTATA ATGCCCTAGA GAAAGCTCGT GTAGCAAATC AAGATCTTCA GGTACAGTTG
    GACCAGGCAC TCCAGCAAGC CTTGGATCCC AATAGTAAAG GCAACTCTTT GTTTGCAGAG
    GTGGAAGATC GAAGGGCAGC AATGGAACGT CAGCTCATCA GTATGAAAGT CAAGTATCAG
    TCACTAAAGA AGCAAAATGT ATTTAACAGA GAACAGATGC AGAGAATGAA GTTACAAATT
    GCCACGTTGC TACAGATGAA AGGGTCTCAA ACTGAATTTG AGCAGCAGGA ACGGTTGCTT
    GCCATGTTGG AGCAGAAGAA TGGTGAAATA AAACATCTTT TAGGTGAAAT TAGAAATCTG
    GAGAAATTTA AGAATTTATA TGACAGTATG GAATCTAAGC CTTCAGTCGA CTCTGGTACT
    CTGGAAGATA ACACCTATTA TACAGATTTA CTTCAGATGA AGCTGGATAA CTTAAACAAA
    GAAATTGAAA GCACTAAAGG TGAATTGTCC ATACAGCGAA TGAAAGCATT ATTTGAGAGC
    CAGCGGGCTC TAGATATTGA GCGAAAACTT TTTGCAAATG AAAGATGCCT CCAGCTTTCA
    GAAAGTGAAA ATATGAAACT GAGAGCTAAA CTAGATGAAT TGAAACTAAA ATATGAACCT
    GAAGAGACAG TTGAAGTGCC TGTACTGAAA AAGAGGCGTG AGGTGCTCCC TGTGGATATA
    ACCACCGCTA AAGATGCATG TGTCAACAAC AGTGCTCTCG GGGGAGAAGT TTATCGATTA
    CCGCCTCAGA AAGAGGAGAC ACAGTCCTGC CCTAACAGTT TAGAAGATAA CAACTTGCAA
    TTAGAAAAAT CAGTTTCTAT ATACACACCA GTAGTCAGTC TCTCTCCTCA CAAAAATCTG
    CCCGTGGATA TGCAGCTGAA GAAGGAAAAG AAATGTGTGA AACTCATAGG AGTTCCCGCT
    GACGCTGAGG CCTTAAGTGA AAGAAGTGGA AACACCCCTA ACTCTCCCAG GTCAGTGTCC
    TCTTTTCCTC CAGGCAGCCA GCAGACCTCT CCATCTCTCC TCTCTCGCTG CATGAACTGT
    GCTGTCTGTT TCTTTATCTA CTTTCTTACA ATTGCATGCA GTATAATTCC TCAGTTTCAT
    CTACCTACCT TCAACTTTTC CAGAACTTTA AGAAAGACTA AACTGATTGC AAAGGGAAAG
    GACTCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu68773
    Clone ID Related Accession (Same CDS sequence) XM_011534589.2 , XM_011534587.2 , NM_001329640.1 , NM_001329639.2 , NM_001329639.1 , NM_001329640.2 , XM_011534588.2
    Accession Version XM_011534587.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1869bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein Spindly isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011534587.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGAGGCAG ATATAATCAC AAATCTTCGA TGCAGGCTCA AAGAGGCTGA AGAAGAGCGA 
    CTAAAAGCTG CACAGTATGG TTTACAACTA GTAGAGAGTC AAAATGAATT ACAGAATCAA
    TTGGATAAAT GTCGTAATGA AATGATGACC ATGACTGAGA GTTATGAACA AGAAAAATAT
    ACCCTTCAAA GAGAAGTTGA ACTCAAGAGT CGAATGTTAG AAAGTTTGAG CTGCGAATGT
    GAAGCTATTA AACAACAACA AAAAATGCAC CTGGAGAAAT TGGAAGAACA ACTAAGCAGA
    AGCCATGGAC AGGAAGTGAA TGAACTAAAA ACTAAGATAG AAAAACTGAA AGTGGAATTA
    GATGAAGCCA GGCTTAGTGA AAAGCAGCTG AAGCACCAAG TAGATCATCA GAAGGAACTC
    CTCTCTTGTA AATCAGAGGA ACTGCGCGTA ATGTCTGAAC GTGTGCAGGA AAGCATGTCT
    TCAGAGATGC TGGCTCTTCA AATTGAGCTG ACAGAAATGG AGAGTATGAA GACCACCCTC
    AAAGAAGAAG TGAATGAACT ACAATACAGA CAAGAACAGC TAGAACTTCT TATTACTAAC
    CTAATGCGCC AGGTAGACCG GCTTAAAGAG GAAAAAGAGG AGCGAGAGAA AGAAGCAGTT
    TCTTACTATA ATGCCCTAGA GAAAGCTCGT GTAGCAAATC AAGATCTTCA GGTACAGTTG
    GACCAGGCAC TCCAGCAAGC CTTGGATCCC AATAGTAAAG GCAACTCTTT GTTTGCAGAG
    GTGGAAGATC GAAGGGCAGC AATGGAACGT CAGCTCATCA GTATGAAAGT CAAGTATCAG
    TCACTAAAGA AGCAAAATGT ATTTAACAGA GAACAGATGC AGAGAATGAA GTTACAAATT
    GCCACGTTGC TACAGATGAA AGGGTCTCAA ACTGAATTTG AGCAGCAGGA ACGGTTGCTT
    GCCATGTTGG AGCAGAAGAA TGGTGAAATA AAACATCTTT TAGGTGAAAT TAGAAATCTG
    GAGAAATTTA AGAATTTATA TGACAGTATG GAATCTAAGC CTTCAGTCGA CTCTGGTACT
    CTGGAAGATA ACACCTATTA TACAGATTTA CTTCAGATGA AGCTGGATAA CTTAAACAAA
    GAAATTGAAA GCACTAAAGG TGAATTGTCC ATACAGCGAA TGAAAGCATT ATTTGAGAGC
    CAGCGGGCTC TAGATATTGA GCGAAAACTT TTTGCAAATG AAAGATGCCT CCAGCTTTCA
    GAAAGTGAAA ATATGAAACT GAGAGCTAAA CTAGATGAAT TGAAACTAAA ATATGAACCT
    GAAGAGACAG TTGAAGTGCC TGTACTGAAA AAGAGGCGTG AGGTGCTCCC TGTGGATATA
    ACCACCGCTA AAGATGCATG TGTCAACAAC AGTGCTCTCG GGGGAGAAGT TTATCGATTA
    CCGCCTCAGA AAGAGGAGAC ACAGTCCTGC CCTAACAGTT TAGAAGATAA CAACTTGCAA
    TTAGAAAAAT CAGTTTCTAT ATACACACCA GTAGTCAGTC TCTCTCCTCA CAAAAATCTG
    CCCGTGGATA TGCAGCTGAA GAAGGAAAAG AAATGTGTGA AACTCATAGG AGTTCCCGCT
    GACGCTGAGG CCTTAAGTGA AAGAAGTGGA AACACCCCTA ACTCTCCCAG GTCAGTGTCC
    TCTTTTCCTC CAGGCAGCCA GCAGACCTCT CCATCTCTCC TCTCTCGCTG CATGAACTGT
    GCTGTCTGTT TCTTTATCTA CTTTCTTACA ATTGCATGCA GTATAATTCC TCAGTTTCAT
    CTACCTACCT TCAACTTTTC CAGAACTTTA AGAAAGACTA AACTGATTGC AAAGGGAAAG
    GACTCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011532889.1
    CDS427..2295
    Translation

    Target ORF information:

    RefSeq Version XM_011534587.2
    Organism Homo sapiens(human)
    Definition Homo sapiens spindle apparatus coiled-coil protein 1 (SPDL1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011534587.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGAGGCAG ATATAATCAC AAATCTTCGA TGCAGGCTCA AAGAGGCTGA AGAAGAGCGA 
    CTAAAAGCTG CACAGTATGG TTTACAACTA GTAGAGAGTC AAAATGAATT ACAGAATCAA
    TTGGATAAAT GTCGTAATGA AATGATGACC ATGACTGAGA GTTATGAACA AGAAAAATAT
    ACCCTTCAAA GAGAAGTTGA ACTCAAGAGT CGAATGTTAG AAAGTTTGAG CTGCGAATGT
    GAAGCTATTA AACAACAACA AAAAATGCAC CTGGAGAAAT TGGAAGAACA ACTAAGCAGA
    AGCCATGGAC AGGAAGTGAA TGAACTAAAA ACTAAGATAG AAAAACTGAA AGTGGAATTA
    GATGAAGCCA GGCTTAGTGA AAAGCAGCTG AAGCACCAAG TAGATCATCA GAAGGAACTC
    CTCTCTTGTA AATCAGAGGA ACTGCGCGTA ATGTCTGAAC GTGTGCAGGA AAGCATGTCT
    TCAGAGATGC TGGCTCTTCA AATTGAGCTG ACAGAAATGG AGAGTATGAA GACCACCCTC
    AAAGAAGAAG TGAATGAACT ACAATACAGA CAAGAACAGC TAGAACTTCT TATTACTAAC
    CTAATGCGCC AGGTAGACCG GCTTAAAGAG GAAAAAGAGG AGCGAGAGAA AGAAGCAGTT
    TCTTACTATA ATGCCCTAGA GAAAGCTCGT GTAGCAAATC AAGATCTTCA GGTACAGTTG
    GACCAGGCAC TCCAGCAAGC CTTGGATCCC AATAGTAAAG GCAACTCTTT GTTTGCAGAG
    GTGGAAGATC GAAGGGCAGC AATGGAACGT CAGCTCATCA GTATGAAAGT CAAGTATCAG
    TCACTAAAGA AGCAAAATGT ATTTAACAGA GAACAGATGC AGAGAATGAA GTTACAAATT
    GCCACGTTGC TACAGATGAA AGGGTCTCAA ACTGAATTTG AGCAGCAGGA ACGGTTGCTT
    GCCATGTTGG AGCAGAAGAA TGGTGAAATA AAACATCTTT TAGGTGAAAT TAGAAATCTG
    GAGAAATTTA AGAATTTATA TGACAGTATG GAATCTAAGC CTTCAGTCGA CTCTGGTACT
    CTGGAAGATA ACACCTATTA TACAGATTTA CTTCAGATGA AGCTGGATAA CTTAAACAAA
    GAAATTGAAA GCACTAAAGG TGAATTGTCC ATACAGCGAA TGAAAGCATT ATTTGAGAGC
    CAGCGGGCTC TAGATATTGA GCGAAAACTT TTTGCAAATG AAAGATGCCT CCAGCTTTCA
    GAAAGTGAAA ATATGAAACT GAGAGCTAAA CTAGATGAAT TGAAACTAAA ATATGAACCT
    GAAGAGACAG TTGAAGTGCC TGTACTGAAA AAGAGGCGTG AGGTGCTCCC TGTGGATATA
    ACCACCGCTA AAGATGCATG TGTCAACAAC AGTGCTCTCG GGGGAGAAGT TTATCGATTA
    CCGCCTCAGA AAGAGGAGAC ACAGTCCTGC CCTAACAGTT TAGAAGATAA CAACTTGCAA
    TTAGAAAAAT CAGTTTCTAT ATACACACCA GTAGTCAGTC TCTCTCCTCA CAAAAATCTG
    CCCGTGGATA TGCAGCTGAA GAAGGAAAAG AAATGTGTGA AACTCATAGG AGTTCCCGCT
    GACGCTGAGG CCTTAAGTGA AAGAAGTGGA AACACCCCTA ACTCTCCCAG GTCAGTGTCC
    TCTTTTCCTC CAGGCAGCCA GCAGACCTCT CCATCTCTCC TCTCTCGCTG CATGAACTGT
    GCTGTCTGTT TCTTTATCTA CTTTCTTACA ATTGCATGCA GTATAATTCC TCAGTTTCAT
    CTACCTACCT TCAACTTTTC CAGAACTTTA AGAAAGACTA AACTGATTGC AAAGGGAAAG
    GACTCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu68773
    Clone ID Related Accession (Same CDS sequence) XM_011534589.2 , XM_011534587.2 , NM_001329640.1 , NM_001329639.2 , NM_001329639.1 , NM_001329640.2 , XM_011534588.2
    Accession Version XM_011534588.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1869bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein Spindly isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011534588.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGAGGCAG ATATAATCAC AAATCTTCGA TGCAGGCTCA AAGAGGCTGA AGAAGAGCGA 
    CTAAAAGCTG CACAGTATGG TTTACAACTA GTAGAGAGTC AAAATGAATT ACAGAATCAA
    TTGGATAAAT GTCGTAATGA AATGATGACC ATGACTGAGA GTTATGAACA AGAAAAATAT
    ACCCTTCAAA GAGAAGTTGA ACTCAAGAGT CGAATGTTAG AAAGTTTGAG CTGCGAATGT
    GAAGCTATTA AACAACAACA AAAAATGCAC CTGGAGAAAT TGGAAGAACA ACTAAGCAGA
    AGCCATGGAC AGGAAGTGAA TGAACTAAAA ACTAAGATAG AAAAACTGAA AGTGGAATTA
    GATGAAGCCA GGCTTAGTGA AAAGCAGCTG AAGCACCAAG TAGATCATCA GAAGGAACTC
    CTCTCTTGTA AATCAGAGGA ACTGCGCGTA ATGTCTGAAC GTGTGCAGGA AAGCATGTCT
    TCAGAGATGC TGGCTCTTCA AATTGAGCTG ACAGAAATGG AGAGTATGAA GACCACCCTC
    AAAGAAGAAG TGAATGAACT ACAATACAGA CAAGAACAGC TAGAACTTCT TATTACTAAC
    CTAATGCGCC AGGTAGACCG GCTTAAAGAG GAAAAAGAGG AGCGAGAGAA AGAAGCAGTT
    TCTTACTATA ATGCCCTAGA GAAAGCTCGT GTAGCAAATC AAGATCTTCA GGTACAGTTG
    GACCAGGCAC TCCAGCAAGC CTTGGATCCC AATAGTAAAG GCAACTCTTT GTTTGCAGAG
    GTGGAAGATC GAAGGGCAGC AATGGAACGT CAGCTCATCA GTATGAAAGT CAAGTATCAG
    TCACTAAAGA AGCAAAATGT ATTTAACAGA GAACAGATGC AGAGAATGAA GTTACAAATT
    GCCACGTTGC TACAGATGAA AGGGTCTCAA ACTGAATTTG AGCAGCAGGA ACGGTTGCTT
    GCCATGTTGG AGCAGAAGAA TGGTGAAATA AAACATCTTT TAGGTGAAAT TAGAAATCTG
    GAGAAATTTA AGAATTTATA TGACAGTATG GAATCTAAGC CTTCAGTCGA CTCTGGTACT
    CTGGAAGATA ACACCTATTA TACAGATTTA CTTCAGATGA AGCTGGATAA CTTAAACAAA
    GAAATTGAAA GCACTAAAGG TGAATTGTCC ATACAGCGAA TGAAAGCATT ATTTGAGAGC
    CAGCGGGCTC TAGATATTGA GCGAAAACTT TTTGCAAATG AAAGATGCCT CCAGCTTTCA
    GAAAGTGAAA ATATGAAACT GAGAGCTAAA CTAGATGAAT TGAAACTAAA ATATGAACCT
    GAAGAGACAG TTGAAGTGCC TGTACTGAAA AAGAGGCGTG AGGTGCTCCC TGTGGATATA
    ACCACCGCTA AAGATGCATG TGTCAACAAC AGTGCTCTCG GGGGAGAAGT TTATCGATTA
    CCGCCTCAGA AAGAGGAGAC ACAGTCCTGC CCTAACAGTT TAGAAGATAA CAACTTGCAA
    TTAGAAAAAT CAGTTTCTAT ATACACACCA GTAGTCAGTC TCTCTCCTCA CAAAAATCTG
    CCCGTGGATA TGCAGCTGAA GAAGGAAAAG AAATGTGTGA AACTCATAGG AGTTCCCGCT
    GACGCTGAGG CCTTAAGTGA AAGAAGTGGA AACACCCCTA ACTCTCCCAG GTCAGTGTCC
    TCTTTTCCTC CAGGCAGCCA GCAGACCTCT CCATCTCTCC TCTCTCGCTG CATGAACTGT
    GCTGTCTGTT TCTTTATCTA CTTTCTTACA ATTGCATGCA GTATAATTCC TCAGTTTCAT
    CTACCTACCT TCAACTTTTC CAGAACTTTA AGAAAGACTA AACTGATTGC AAAGGGAAAG
    GACTCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011532890.1
    CDS365..2233
    Translation

    Target ORF information:

    RefSeq Version XM_011534588.2
    Organism Homo sapiens(human)
    Definition Homo sapiens spindle apparatus coiled-coil protein 1 (SPDL1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011534588.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGAGGCAG ATATAATCAC AAATCTTCGA TGCAGGCTCA AAGAGGCTGA AGAAGAGCGA 
    CTAAAAGCTG CACAGTATGG TTTACAACTA GTAGAGAGTC AAAATGAATT ACAGAATCAA
    TTGGATAAAT GTCGTAATGA AATGATGACC ATGACTGAGA GTTATGAACA AGAAAAATAT
    ACCCTTCAAA GAGAAGTTGA ACTCAAGAGT CGAATGTTAG AAAGTTTGAG CTGCGAATGT
    GAAGCTATTA AACAACAACA AAAAATGCAC CTGGAGAAAT TGGAAGAACA ACTAAGCAGA
    AGCCATGGAC AGGAAGTGAA TGAACTAAAA ACTAAGATAG AAAAACTGAA AGTGGAATTA
    GATGAAGCCA GGCTTAGTGA AAAGCAGCTG AAGCACCAAG TAGATCATCA GAAGGAACTC
    CTCTCTTGTA AATCAGAGGA ACTGCGCGTA ATGTCTGAAC GTGTGCAGGA AAGCATGTCT
    TCAGAGATGC TGGCTCTTCA AATTGAGCTG ACAGAAATGG AGAGTATGAA GACCACCCTC
    AAAGAAGAAG TGAATGAACT ACAATACAGA CAAGAACAGC TAGAACTTCT TATTACTAAC
    CTAATGCGCC AGGTAGACCG GCTTAAAGAG GAAAAAGAGG AGCGAGAGAA AGAAGCAGTT
    TCTTACTATA ATGCCCTAGA GAAAGCTCGT GTAGCAAATC AAGATCTTCA GGTACAGTTG
    GACCAGGCAC TCCAGCAAGC CTTGGATCCC AATAGTAAAG GCAACTCTTT GTTTGCAGAG
    GTGGAAGATC GAAGGGCAGC AATGGAACGT CAGCTCATCA GTATGAAAGT CAAGTATCAG
    TCACTAAAGA AGCAAAATGT ATTTAACAGA GAACAGATGC AGAGAATGAA GTTACAAATT
    GCCACGTTGC TACAGATGAA AGGGTCTCAA ACTGAATTTG AGCAGCAGGA ACGGTTGCTT
    GCCATGTTGG AGCAGAAGAA TGGTGAAATA AAACATCTTT TAGGTGAAAT TAGAAATCTG
    GAGAAATTTA AGAATTTATA TGACAGTATG GAATCTAAGC CTTCAGTCGA CTCTGGTACT
    CTGGAAGATA ACACCTATTA TACAGATTTA CTTCAGATGA AGCTGGATAA CTTAAACAAA
    GAAATTGAAA GCACTAAAGG TGAATTGTCC ATACAGCGAA TGAAAGCATT ATTTGAGAGC
    CAGCGGGCTC TAGATATTGA GCGAAAACTT TTTGCAAATG AAAGATGCCT CCAGCTTTCA
    GAAAGTGAAA ATATGAAACT GAGAGCTAAA CTAGATGAAT TGAAACTAAA ATATGAACCT
    GAAGAGACAG TTGAAGTGCC TGTACTGAAA AAGAGGCGTG AGGTGCTCCC TGTGGATATA
    ACCACCGCTA AAGATGCATG TGTCAACAAC AGTGCTCTCG GGGGAGAAGT TTATCGATTA
    CCGCCTCAGA AAGAGGAGAC ACAGTCCTGC CCTAACAGTT TAGAAGATAA CAACTTGCAA
    TTAGAAAAAT CAGTTTCTAT ATACACACCA GTAGTCAGTC TCTCTCCTCA CAAAAATCTG
    CCCGTGGATA TGCAGCTGAA GAAGGAAAAG AAATGTGTGA AACTCATAGG AGTTCCCGCT
    GACGCTGAGG CCTTAAGTGA AAGAAGTGGA AACACCCCTA ACTCTCCCAG GTCAGTGTCC
    TCTTTTCCTC CAGGCAGCCA GCAGACCTCT CCATCTCTCC TCTCTCGCTG CATGAACTGT
    GCTGTCTGTT TCTTTATCTA CTTTCTTACA ATTGCATGCA GTATAATTCC TCAGTTTCAT
    CTACCTACCT TCAACTTTTC CAGAACTTTA AGAAAGACTA AACTGATTGC AAAGGGAAAG
    GACTCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu31741
    Clone ID Related Accession (Same CDS sequence) NM_017785.4 , NM_001329641.1 , NM_017785.5 , NM_001329641.2
    Accession Version NM_017785.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1818bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-11-26
    Organism Homo sapiens(human)
    Product protein Spindly isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC379275.1, BC012568.1, BU166207.1, CD653505.1, BP295943.1 and CA417197.1. This sequence is a reference standard in the RefSeqGene project. On Sep 20, 2007 this sequence version replaced NM_017785.3. Transcript Variant: Variants 3 and 4 encode the same isoform (b). ##Evidence-Data-START## Transcript exon combination :: SRR1803616.269176.1, SRR1803613.384.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGAGGCAG ATATAATCAC AAATCTTCGA TGCAGGCTCA AAGAGGCTGA AGAAGAGCGA 
    CTAAAAGCTG CACAGTATGG TTTACAACTA GTAGAGAGTC AAAATGAATT ACAGAATCAA
    TTGGATAAAT GTCGTAATGA AATGATGACC ATGACTGAGA GTTATGAACA AGAAAAATAT
    ACCCTTCAAA GAGAAGTTGA ACTCAAGAGT CGAATGTTAG AAAGTTTGAG CTGCGAATGT
    GAAGCTATTA AACAACAACA AAAAATGCAC CTGGAGAAAT TGGAAGAACA ACTAAGCAGA
    AGCCATGGAC AGGAAGTGAA TGAACTAAAA ACTAAGATAG AAAAACTGAA AGTGGAATTA
    GATGAAGCCA GGCTTAGTGA AAAGCAGCTG AAGCACCAAG TAGATCATCA GAAGGAACTC
    CTCTCTTGTA AATCAGAGGA ACTGCGCGTA ATGTCTGAAC GTGTGCAGGA AAGCATGTCT
    TCAGAGATGC TGGCTCTTCA AATTGAGCTG ACAGAAATGG AGAGTATGAA GACCACCCTC
    AAAGAAGAAG TGAATGAACT ACAATACAGA CAAGAACAGC TAGAACTTCT TATTACTAAC
    CTAATGCGCC AGGTAGACCG GCTTAAAGAG GAAAAAGAGG AGCGAGAGAA AGAAGCAGTT
    TCTTACTATA ATGCCCTAGA GAAAGCTCGT GTAGCAAATC AAGATCTTCA GGTACAGTTG
    GACCAGGCAC TCCAGCAAGC CTTGGATCCC AATAGTAAAG GCAACTCTTT GTTTGCAGAG
    GTGGAAGATC GAAGGGCAGC AATGGAACGT CAGCTCATCA GTATGAAAGT CAAGTATCAG
    TCACTAAAGA AGCAAAATGT ATTTAACAGA GAACAGATGC AGAGAATGAA GTTACAAATT
    GCCACGTTGC TACAGATGAA AGGGTCTCAA ACTGAATTTG AGCAGCAGGA ACGGTTGCTT
    GCCATGTTGG AGCAGAAGAA TGGTGAAATA AAACATCTTT TAGGTGAAAT TAGAAATCTG
    GAGAAATTTA AGAATTTATA TGACAGTATG GAATCTAAGC CTTCAGTCGA CTCTGGTACT
    CTGGAAGATA ACACCTATTA TACAGATTTA CTTCAGATGA AGCTGGATAA CTTAAACAAA
    GAAATTGAAA GCACTAAAGG TGAATTGTCC ATACAGCGAA TGAAAGCATT ATTTGAGAGC
    CAGCGGGCTC TAGATATTGA GCGAAAACTT TTTGCAAATG AAAGATGCCT CCAGCTTTCA
    GAAAGTGAAA ATATGAAACT GAGAGCTAAA CTAGATGAAT TGAAACTAAA ATATGAACCT
    GAAGAGACAG TTGAAGTGCC TGTACTGAAA AAGAGGCGTG AGGTGCTCCC TGTGGATATA
    ACCACCGCTA AAGATGCATG TGTCAACAAC AGTGCTCTCG GGGGAGAAGT TTATCGATTA
    CCGCCTCAGA AAGAGGAGAC ACAGTCCTGC CCTAACAGTT TAGAAGATAA CAACTTGCAA
    TTAGAAAAAT CAGTTTCTAT ATACACACCA GTAGTCAGTC TCTCTCCTCA CAAAAATCTG
    CCCGTGGATA TGCAGCTGAA GAAGGAAAAG AAATGTGTGA AACTCATAGG AGTTCCCGCT
    GACGCTGAGG CCTTAAGTGA AAGAAGTGGA AACACCCCTA ACTCTCCCAG GTTAGCTGCT
    GAATCAAAGC TTCAAACAGA AGTTAAAGAA GGAAAAGAAA CTTCAAGCAA ATTGGAAAAA
    GAAACTTGTA AGAAATTACA CCCTATTCTA TATGTGTCTT CTAAATCTAC TCCAGAGACC
    CAGTGCCCTC AACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_060255.3
    CDS280..2097
    Translation

    Target ORF information:

    RefSeq Version NM_017785.4
    Organism Homo sapiens(human)
    Definition Homo sapiens spindle apparatus coiled-coil protein 1 (SPDL1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_017785.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGAGGCAG ATATAATCAC AAATCTTCGA TGCAGGCTCA AAGAGGCTGA AGAAGAGCGA 
    CTAAAAGCTG CACAGTATGG TTTACAACTA GTAGAGAGTC AAAATGAATT ACAGAATCAA
    TTGGATAAAT GTCGTAATGA AATGATGACC ATGACTGAGA GTTATGAACA AGAAAAATAT
    ACCCTTCAAA GAGAAGTTGA ACTCAAGAGT CGAATGTTAG AAAGTTTGAG CTGCGAATGT
    GAAGCTATTA AACAACAACA AAAAATGCAC CTGGAGAAAT TGGAAGAACA ACTAAGCAGA
    AGCCATGGAC AGGAAGTGAA TGAACTAAAA ACTAAGATAG AAAAACTGAA AGTGGAATTA
    GATGAAGCCA GGCTTAGTGA AAAGCAGCTG AAGCACCAAG TAGATCATCA GAAGGAACTC
    CTCTCTTGTA AATCAGAGGA ACTGCGCGTA ATGTCTGAAC GTGTGCAGGA AAGCATGTCT
    TCAGAGATGC TGGCTCTTCA AATTGAGCTG ACAGAAATGG AGAGTATGAA GACCACCCTC
    AAAGAAGAAG TGAATGAACT ACAATACAGA CAAGAACAGC TAGAACTTCT TATTACTAAC
    CTAATGCGCC AGGTAGACCG GCTTAAAGAG GAAAAAGAGG AGCGAGAGAA AGAAGCAGTT
    TCTTACTATA ATGCCCTAGA GAAAGCTCGT GTAGCAAATC AAGATCTTCA GGTACAGTTG
    GACCAGGCAC TCCAGCAAGC CTTGGATCCC AATAGTAAAG GCAACTCTTT GTTTGCAGAG
    GTGGAAGATC GAAGGGCAGC AATGGAACGT CAGCTCATCA GTATGAAAGT CAAGTATCAG
    TCACTAAAGA AGCAAAATGT ATTTAACAGA GAACAGATGC AGAGAATGAA GTTACAAATT
    GCCACGTTGC TACAGATGAA AGGGTCTCAA ACTGAATTTG AGCAGCAGGA ACGGTTGCTT
    GCCATGTTGG AGCAGAAGAA TGGTGAAATA AAACATCTTT TAGGTGAAAT TAGAAATCTG
    GAGAAATTTA AGAATTTATA TGACAGTATG GAATCTAAGC CTTCAGTCGA CTCTGGTACT
    CTGGAAGATA ACACCTATTA TACAGATTTA CTTCAGATGA AGCTGGATAA CTTAAACAAA
    GAAATTGAAA GCACTAAAGG TGAATTGTCC ATACAGCGAA TGAAAGCATT ATTTGAGAGC
    CAGCGGGCTC TAGATATTGA GCGAAAACTT TTTGCAAATG AAAGATGCCT CCAGCTTTCA
    GAAAGTGAAA ATATGAAACT GAGAGCTAAA CTAGATGAAT TGAAACTAAA ATATGAACCT
    GAAGAGACAG TTGAAGTGCC TGTACTGAAA AAGAGGCGTG AGGTGCTCCC TGTGGATATA
    ACCACCGCTA AAGATGCATG TGTCAACAAC AGTGCTCTCG GGGGAGAAGT TTATCGATTA
    CCGCCTCAGA AAGAGGAGAC ACAGTCCTGC CCTAACAGTT TAGAAGATAA CAACTTGCAA
    TTAGAAAAAT CAGTTTCTAT ATACACACCA GTAGTCAGTC TCTCTCCTCA CAAAAATCTG
    CCCGTGGATA TGCAGCTGAA GAAGGAAAAG AAATGTGTGA AACTCATAGG AGTTCCCGCT
    GACGCTGAGG CCTTAAGTGA AAGAAGTGGA AACACCCCTA ACTCTCCCAG GTTAGCTGCT
    GAATCAAAGC TTCAAACAGA AGTTAAAGAA GGAAAAGAAA CTTCAAGCAA ATTGGAAAAA
    GAAACTTGTA AGAAATTACA CCCTATTCTA TATGTGTCTT CTAAATCTAC TCCAGAGACC
    CAGTGCCCTC AACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu31741
    Clone ID Related Accession (Same CDS sequence) NM_017785.4 , NM_001329641.1 , NM_017785.5 , NM_001329641.2
    Accession Version NM_001329641.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1818bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-25
    Organism Homo sapiens(human)
    Product protein Spindly isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008680.5 and BU616897.1. On Jul 21, 2016 this sequence version replaced XM_017009601.1. Transcript Variant: Variants 3 and 4 encode the same isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.126268.1, SRR1803616.78644.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGAGGCAG ATATAATCAC AAATCTTCGA TGCAGGCTCA AAGAGGCTGA AGAAGAGCGA 
    CTAAAAGCTG CACAGTATGG TTTACAACTA GTAGAGAGTC AAAATGAATT ACAGAATCAA
    TTGGATAAAT GTCGTAATGA AATGATGACC ATGACTGAGA GTTATGAACA AGAAAAATAT
    ACCCTTCAAA GAGAAGTTGA ACTCAAGAGT CGAATGTTAG AAAGTTTGAG CTGCGAATGT
    GAAGCTATTA AACAACAACA AAAAATGCAC CTGGAGAAAT TGGAAGAACA ACTAAGCAGA
    AGCCATGGAC AGGAAGTGAA TGAACTAAAA ACTAAGATAG AAAAACTGAA AGTGGAATTA
    GATGAAGCCA GGCTTAGTGA AAAGCAGCTG AAGCACCAAG TAGATCATCA GAAGGAACTC
    CTCTCTTGTA AATCAGAGGA ACTGCGCGTA ATGTCTGAAC GTGTGCAGGA AAGCATGTCT
    TCAGAGATGC TGGCTCTTCA AATTGAGCTG ACAGAAATGG AGAGTATGAA GACCACCCTC
    AAAGAAGAAG TGAATGAACT ACAATACAGA CAAGAACAGC TAGAACTTCT TATTACTAAC
    CTAATGCGCC AGGTAGACCG GCTTAAAGAG GAAAAAGAGG AGCGAGAGAA AGAAGCAGTT
    TCTTACTATA ATGCCCTAGA GAAAGCTCGT GTAGCAAATC AAGATCTTCA GGTACAGTTG
    GACCAGGCAC TCCAGCAAGC CTTGGATCCC AATAGTAAAG GCAACTCTTT GTTTGCAGAG
    GTGGAAGATC GAAGGGCAGC AATGGAACGT CAGCTCATCA GTATGAAAGT CAAGTATCAG
    TCACTAAAGA AGCAAAATGT ATTTAACAGA GAACAGATGC AGAGAATGAA GTTACAAATT
    GCCACGTTGC TACAGATGAA AGGGTCTCAA ACTGAATTTG AGCAGCAGGA ACGGTTGCTT
    GCCATGTTGG AGCAGAAGAA TGGTGAAATA AAACATCTTT TAGGTGAAAT TAGAAATCTG
    GAGAAATTTA AGAATTTATA TGACAGTATG GAATCTAAGC CTTCAGTCGA CTCTGGTACT
    CTGGAAGATA ACACCTATTA TACAGATTTA CTTCAGATGA AGCTGGATAA CTTAAACAAA
    GAAATTGAAA GCACTAAAGG TGAATTGTCC ATACAGCGAA TGAAAGCATT ATTTGAGAGC
    CAGCGGGCTC TAGATATTGA GCGAAAACTT TTTGCAAATG AAAGATGCCT CCAGCTTTCA
    GAAAGTGAAA ATATGAAACT GAGAGCTAAA CTAGATGAAT TGAAACTAAA ATATGAACCT
    GAAGAGACAG TTGAAGTGCC TGTACTGAAA AAGAGGCGTG AGGTGCTCCC TGTGGATATA
    ACCACCGCTA AAGATGCATG TGTCAACAAC AGTGCTCTCG GGGGAGAAGT TTATCGATTA
    CCGCCTCAGA AAGAGGAGAC ACAGTCCTGC CCTAACAGTT TAGAAGATAA CAACTTGCAA
    TTAGAAAAAT CAGTTTCTAT ATACACACCA GTAGTCAGTC TCTCTCCTCA CAAAAATCTG
    CCCGTGGATA TGCAGCTGAA GAAGGAAAAG AAATGTGTGA AACTCATAGG AGTTCCCGCT
    GACGCTGAGG CCTTAAGTGA AAGAAGTGGA AACACCCCTA ACTCTCCCAG GTTAGCTGCT
    GAATCAAAGC TTCAAACAGA AGTTAAAGAA GGAAAAGAAA CTTCAAGCAA ATTGGAAAAA
    GAAACTTGTA AGAAATTACA CCCTATTCTA TATGTGTCTT CTAAATCTAC TCCAGAGACC
    CAGTGCCCTC AACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001316570.1
    CDS111..1928
    Translation

    Target ORF information:

    RefSeq Version NM_001329641.1
    Organism Homo sapiens(human)
    Definition Homo sapiens spindle apparatus coiled-coil protein 1 (SPDL1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001329641.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGAGGCAG ATATAATCAC AAATCTTCGA TGCAGGCTCA AAGAGGCTGA AGAAGAGCGA 
    CTAAAAGCTG CACAGTATGG TTTACAACTA GTAGAGAGTC AAAATGAATT ACAGAATCAA
    TTGGATAAAT GTCGTAATGA AATGATGACC ATGACTGAGA GTTATGAACA AGAAAAATAT
    ACCCTTCAAA GAGAAGTTGA ACTCAAGAGT CGAATGTTAG AAAGTTTGAG CTGCGAATGT
    GAAGCTATTA AACAACAACA AAAAATGCAC CTGGAGAAAT TGGAAGAACA ACTAAGCAGA
    AGCCATGGAC AGGAAGTGAA TGAACTAAAA ACTAAGATAG AAAAACTGAA AGTGGAATTA
    GATGAAGCCA GGCTTAGTGA AAAGCAGCTG AAGCACCAAG TAGATCATCA GAAGGAACTC
    CTCTCTTGTA AATCAGAGGA ACTGCGCGTA ATGTCTGAAC GTGTGCAGGA AAGCATGTCT
    TCAGAGATGC TGGCTCTTCA AATTGAGCTG ACAGAAATGG AGAGTATGAA GACCACCCTC
    AAAGAAGAAG TGAATGAACT ACAATACAGA CAAGAACAGC TAGAACTTCT TATTACTAAC
    CTAATGCGCC AGGTAGACCG GCTTAAAGAG GAAAAAGAGG AGCGAGAGAA AGAAGCAGTT
    TCTTACTATA ATGCCCTAGA GAAAGCTCGT GTAGCAAATC AAGATCTTCA GGTACAGTTG
    GACCAGGCAC TCCAGCAAGC CTTGGATCCC AATAGTAAAG GCAACTCTTT GTTTGCAGAG
    GTGGAAGATC GAAGGGCAGC AATGGAACGT CAGCTCATCA GTATGAAAGT CAAGTATCAG
    TCACTAAAGA AGCAAAATGT ATTTAACAGA GAACAGATGC AGAGAATGAA GTTACAAATT
    GCCACGTTGC TACAGATGAA AGGGTCTCAA ACTGAATTTG AGCAGCAGGA ACGGTTGCTT
    GCCATGTTGG AGCAGAAGAA TGGTGAAATA AAACATCTTT TAGGTGAAAT TAGAAATCTG
    GAGAAATTTA AGAATTTATA TGACAGTATG GAATCTAAGC CTTCAGTCGA CTCTGGTACT
    CTGGAAGATA ACACCTATTA TACAGATTTA CTTCAGATGA AGCTGGATAA CTTAAACAAA
    GAAATTGAAA GCACTAAAGG TGAATTGTCC ATACAGCGAA TGAAAGCATT ATTTGAGAGC
    CAGCGGGCTC TAGATATTGA GCGAAAACTT TTTGCAAATG AAAGATGCCT CCAGCTTTCA
    GAAAGTGAAA ATATGAAACT GAGAGCTAAA CTAGATGAAT TGAAACTAAA ATATGAACCT
    GAAGAGACAG TTGAAGTGCC TGTACTGAAA AAGAGGCGTG AGGTGCTCCC TGTGGATATA
    ACCACCGCTA AAGATGCATG TGTCAACAAC AGTGCTCTCG GGGGAGAAGT TTATCGATTA
    CCGCCTCAGA AAGAGGAGAC ACAGTCCTGC CCTAACAGTT TAGAAGATAA CAACTTGCAA
    TTAGAAAAAT CAGTTTCTAT ATACACACCA GTAGTCAGTC TCTCTCCTCA CAAAAATCTG
    CCCGTGGATA TGCAGCTGAA GAAGGAAAAG AAATGTGTGA AACTCATAGG AGTTCCCGCT
    GACGCTGAGG CCTTAAGTGA AAGAAGTGGA AACACCCCTA ACTCTCCCAG GTTAGCTGCT
    GAATCAAAGC TTCAAACAGA AGTTAAAGAA GGAAAAGAAA CTTCAAGCAA ATTGGAAAAA
    GAAACTTGTA AGAAATTACA CCCTATTCTA TATGTGTCTT CTAAATCTAC TCCAGAGACC
    CAGTGCCCTC AACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu31741
    Clone ID Related Accession (Same CDS sequence) NM_017785.4 , NM_001329641.1 , NM_017785.5 , NM_001329641.2
    Accession Version NM_017785.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1818bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product protein Spindly isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC379275.1, BC012568.1, BU166207.1, CD653505.1, BP295943.1 and CA417197.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_017785.4. Transcript Variant: Variants 3 and 4 encode the same isoform (b). ##Evidence-Data-START## Transcript exon combination :: SRR5189658.167767.1, BC012568.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000265295.9/ ENSP00000265295.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGAGGCAG ATATAATCAC AAATCTTCGA TGCAGGCTCA AAGAGGCTGA AGAAGAGCGA 
    CTAAAAGCTG CACAGTATGG TTTACAACTA GTAGAGAGTC AAAATGAATT ACAGAATCAA
    TTGGATAAAT GTCGTAATGA AATGATGACC ATGACTGAGA GTTATGAACA AGAAAAATAT
    ACCCTTCAAA GAGAAGTTGA ACTCAAGAGT CGAATGTTAG AAAGTTTGAG CTGCGAATGT
    GAAGCTATTA AACAACAACA AAAAATGCAC CTGGAGAAAT TGGAAGAACA ACTAAGCAGA
    AGCCATGGAC AGGAAGTGAA TGAACTAAAA ACTAAGATAG AAAAACTGAA AGTGGAATTA
    GATGAAGCCA GGCTTAGTGA AAAGCAGCTG AAGCACCAAG TAGATCATCA GAAGGAACTC
    CTCTCTTGTA AATCAGAGGA ACTGCGCGTA ATGTCTGAAC GTGTGCAGGA AAGCATGTCT
    TCAGAGATGC TGGCTCTTCA AATTGAGCTG ACAGAAATGG AGAGTATGAA GACCACCCTC
    AAAGAAGAAG TGAATGAACT ACAATACAGA CAAGAACAGC TAGAACTTCT TATTACTAAC
    CTAATGCGCC AGGTAGACCG GCTTAAAGAG GAAAAAGAGG AGCGAGAGAA AGAAGCAGTT
    TCTTACTATA ATGCCCTAGA GAAAGCTCGT GTAGCAAATC AAGATCTTCA GGTACAGTTG
    GACCAGGCAC TCCAGCAAGC CTTGGATCCC AATAGTAAAG GCAACTCTTT GTTTGCAGAG
    GTGGAAGATC GAAGGGCAGC AATGGAACGT CAGCTCATCA GTATGAAAGT CAAGTATCAG
    TCACTAAAGA AGCAAAATGT ATTTAACAGA GAACAGATGC AGAGAATGAA GTTACAAATT
    GCCACGTTGC TACAGATGAA AGGGTCTCAA ACTGAATTTG AGCAGCAGGA ACGGTTGCTT
    GCCATGTTGG AGCAGAAGAA TGGTGAAATA AAACATCTTT TAGGTGAAAT TAGAAATCTG
    GAGAAATTTA AGAATTTATA TGACAGTATG GAATCTAAGC CTTCAGTCGA CTCTGGTACT
    CTGGAAGATA ACACCTATTA TACAGATTTA CTTCAGATGA AGCTGGATAA CTTAAACAAA
    GAAATTGAAA GCACTAAAGG TGAATTGTCC ATACAGCGAA TGAAAGCATT ATTTGAGAGC
    CAGCGGGCTC TAGATATTGA GCGAAAACTT TTTGCAAATG AAAGATGCCT CCAGCTTTCA
    GAAAGTGAAA ATATGAAACT GAGAGCTAAA CTAGATGAAT TGAAACTAAA ATATGAACCT
    GAAGAGACAG TTGAAGTGCC TGTACTGAAA AAGAGGCGTG AGGTGCTCCC TGTGGATATA
    ACCACCGCTA AAGATGCATG TGTCAACAAC AGTGCTCTCG GGGGAGAAGT TTATCGATTA
    CCGCCTCAGA AAGAGGAGAC ACAGTCCTGC CCTAACAGTT TAGAAGATAA CAACTTGCAA
    TTAGAAAAAT CAGTTTCTAT ATACACACCA GTAGTCAGTC TCTCTCCTCA CAAAAATCTG
    CCCGTGGATA TGCAGCTGAA GAAGGAAAAG AAATGTGTGA AACTCATAGG AGTTCCCGCT
    GACGCTGAGG CCTTAAGTGA AAGAAGTGGA AACACCCCTA ACTCTCCCAG GTTAGCTGCT
    GAATCAAAGC TTCAAACAGA AGTTAAAGAA GGAAAAGAAA CTTCAAGCAA ATTGGAAAAA
    GAAACTTGTA AGAAATTACA CCCTATTCTA TATGTGTCTT CTAAATCTAC TCCAGAGACC
    CAGTGCCCTC AACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_060255.3
    CDS141..1958
    Translation

    Target ORF information:

    RefSeq Version NM_017785.5
    Organism Homo sapiens(human)
    Definition Homo sapiens spindle apparatus coiled-coil protein 1 (SPDL1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_017785.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGAGGCAG ATATAATCAC AAATCTTCGA TGCAGGCTCA AAGAGGCTGA AGAAGAGCGA 
    CTAAAAGCTG CACAGTATGG TTTACAACTA GTAGAGAGTC AAAATGAATT ACAGAATCAA
    TTGGATAAAT GTCGTAATGA AATGATGACC ATGACTGAGA GTTATGAACA AGAAAAATAT
    ACCCTTCAAA GAGAAGTTGA ACTCAAGAGT CGAATGTTAG AAAGTTTGAG CTGCGAATGT
    GAAGCTATTA AACAACAACA AAAAATGCAC CTGGAGAAAT TGGAAGAACA ACTAAGCAGA
    AGCCATGGAC AGGAAGTGAA TGAACTAAAA ACTAAGATAG AAAAACTGAA AGTGGAATTA
    GATGAAGCCA GGCTTAGTGA AAAGCAGCTG AAGCACCAAG TAGATCATCA GAAGGAACTC
    CTCTCTTGTA AATCAGAGGA ACTGCGCGTA ATGTCTGAAC GTGTGCAGGA AAGCATGTCT
    TCAGAGATGC TGGCTCTTCA AATTGAGCTG ACAGAAATGG AGAGTATGAA GACCACCCTC
    AAAGAAGAAG TGAATGAACT ACAATACAGA CAAGAACAGC TAGAACTTCT TATTACTAAC
    CTAATGCGCC AGGTAGACCG GCTTAAAGAG GAAAAAGAGG AGCGAGAGAA AGAAGCAGTT
    TCTTACTATA ATGCCCTAGA GAAAGCTCGT GTAGCAAATC AAGATCTTCA GGTACAGTTG
    GACCAGGCAC TCCAGCAAGC CTTGGATCCC AATAGTAAAG GCAACTCTTT GTTTGCAGAG
    GTGGAAGATC GAAGGGCAGC AATGGAACGT CAGCTCATCA GTATGAAAGT CAAGTATCAG
    TCACTAAAGA AGCAAAATGT ATTTAACAGA GAACAGATGC AGAGAATGAA GTTACAAATT
    GCCACGTTGC TACAGATGAA AGGGTCTCAA ACTGAATTTG AGCAGCAGGA ACGGTTGCTT
    GCCATGTTGG AGCAGAAGAA TGGTGAAATA AAACATCTTT TAGGTGAAAT TAGAAATCTG
    GAGAAATTTA AGAATTTATA TGACAGTATG GAATCTAAGC CTTCAGTCGA CTCTGGTACT
    CTGGAAGATA ACACCTATTA TACAGATTTA CTTCAGATGA AGCTGGATAA CTTAAACAAA
    GAAATTGAAA GCACTAAAGG TGAATTGTCC ATACAGCGAA TGAAAGCATT ATTTGAGAGC
    CAGCGGGCTC TAGATATTGA GCGAAAACTT TTTGCAAATG AAAGATGCCT CCAGCTTTCA
    GAAAGTGAAA ATATGAAACT GAGAGCTAAA CTAGATGAAT TGAAACTAAA ATATGAACCT
    GAAGAGACAG TTGAAGTGCC TGTACTGAAA AAGAGGCGTG AGGTGCTCCC TGTGGATATA
    ACCACCGCTA AAGATGCATG TGTCAACAAC AGTGCTCTCG GGGGAGAAGT TTATCGATTA
    CCGCCTCAGA AAGAGGAGAC ACAGTCCTGC CCTAACAGTT TAGAAGATAA CAACTTGCAA
    TTAGAAAAAT CAGTTTCTAT ATACACACCA GTAGTCAGTC TCTCTCCTCA CAAAAATCTG
    CCCGTGGATA TGCAGCTGAA GAAGGAAAAG AAATGTGTGA AACTCATAGG AGTTCCCGCT
    GACGCTGAGG CCTTAAGTGA AAGAAGTGGA AACACCCCTA ACTCTCCCAG GTTAGCTGCT
    GAATCAAAGC TTCAAACAGA AGTTAAAGAA GGAAAAGAAA CTTCAAGCAA ATTGGAAAAA
    GAAACTTGTA AGAAATTACA CCCTATTCTA TATGTGTCTT CTAAATCTAC TCCAGAGACC
    CAGTGCCCTC AACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu31741
    Clone ID Related Accession (Same CDS sequence) NM_017785.4 , NM_001329641.1 , NM_017785.5 , NM_001329641.2
    Accession Version NM_001329641.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1818bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product protein Spindly isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008680.5 and BU616897.1. On May 31, 2019 this sequence version replaced NM_001329641.1. Transcript Variant: Variants 3 and 4 encode the same isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.30776.1, SRR1803613.250487.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGAGGCAG ATATAATCAC AAATCTTCGA TGCAGGCTCA AAGAGGCTGA AGAAGAGCGA 
    CTAAAAGCTG CACAGTATGG TTTACAACTA GTAGAGAGTC AAAATGAATT ACAGAATCAA
    TTGGATAAAT GTCGTAATGA AATGATGACC ATGACTGAGA GTTATGAACA AGAAAAATAT
    ACCCTTCAAA GAGAAGTTGA ACTCAAGAGT CGAATGTTAG AAAGTTTGAG CTGCGAATGT
    GAAGCTATTA AACAACAACA AAAAATGCAC CTGGAGAAAT TGGAAGAACA ACTAAGCAGA
    AGCCATGGAC AGGAAGTGAA TGAACTAAAA ACTAAGATAG AAAAACTGAA AGTGGAATTA
    GATGAAGCCA GGCTTAGTGA AAAGCAGCTG AAGCACCAAG TAGATCATCA GAAGGAACTC
    CTCTCTTGTA AATCAGAGGA ACTGCGCGTA ATGTCTGAAC GTGTGCAGGA AAGCATGTCT
    TCAGAGATGC TGGCTCTTCA AATTGAGCTG ACAGAAATGG AGAGTATGAA GACCACCCTC
    AAAGAAGAAG TGAATGAACT ACAATACAGA CAAGAACAGC TAGAACTTCT TATTACTAAC
    CTAATGCGCC AGGTAGACCG GCTTAAAGAG GAAAAAGAGG AGCGAGAGAA AGAAGCAGTT
    TCTTACTATA ATGCCCTAGA GAAAGCTCGT GTAGCAAATC AAGATCTTCA GGTACAGTTG
    GACCAGGCAC TCCAGCAAGC CTTGGATCCC AATAGTAAAG GCAACTCTTT GTTTGCAGAG
    GTGGAAGATC GAAGGGCAGC AATGGAACGT CAGCTCATCA GTATGAAAGT CAAGTATCAG
    TCACTAAAGA AGCAAAATGT ATTTAACAGA GAACAGATGC AGAGAATGAA GTTACAAATT
    GCCACGTTGC TACAGATGAA AGGGTCTCAA ACTGAATTTG AGCAGCAGGA ACGGTTGCTT
    GCCATGTTGG AGCAGAAGAA TGGTGAAATA AAACATCTTT TAGGTGAAAT TAGAAATCTG
    GAGAAATTTA AGAATTTATA TGACAGTATG GAATCTAAGC CTTCAGTCGA CTCTGGTACT
    CTGGAAGATA ACACCTATTA TACAGATTTA CTTCAGATGA AGCTGGATAA CTTAAACAAA
    GAAATTGAAA GCACTAAAGG TGAATTGTCC ATACAGCGAA TGAAAGCATT ATTTGAGAGC
    CAGCGGGCTC TAGATATTGA GCGAAAACTT TTTGCAAATG AAAGATGCCT CCAGCTTTCA
    GAAAGTGAAA ATATGAAACT GAGAGCTAAA CTAGATGAAT TGAAACTAAA ATATGAACCT
    GAAGAGACAG TTGAAGTGCC TGTACTGAAA AAGAGGCGTG AGGTGCTCCC TGTGGATATA
    ACCACCGCTA AAGATGCATG TGTCAACAAC AGTGCTCTCG GGGGAGAAGT TTATCGATTA
    CCGCCTCAGA AAGAGGAGAC ACAGTCCTGC CCTAACAGTT TAGAAGATAA CAACTTGCAA
    TTAGAAAAAT CAGTTTCTAT ATACACACCA GTAGTCAGTC TCTCTCCTCA CAAAAATCTG
    CCCGTGGATA TGCAGCTGAA GAAGGAAAAG AAATGTGTGA AACTCATAGG AGTTCCCGCT
    GACGCTGAGG CCTTAAGTGA AAGAAGTGGA AACACCCCTA ACTCTCCCAG GTTAGCTGCT
    GAATCAAAGC TTCAAACAGA AGTTAAAGAA GGAAAAGAAA CTTCAAGCAA ATTGGAAAAA
    GAAACTTGTA AGAAATTACA CCCTATTCTA TATGTGTCTT CTAAATCTAC TCCAGAGACC
    CAGTGCCCTC AACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001316570.1
    CDS111..1928
    Translation

    Target ORF information:

    RefSeq Version NM_001329641.2
    Organism Homo sapiens(human)
    Definition Homo sapiens spindle apparatus coiled-coil protein 1 (SPDL1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001329641.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGAGGCAG ATATAATCAC AAATCTTCGA TGCAGGCTCA AAGAGGCTGA AGAAGAGCGA 
    CTAAAAGCTG CACAGTATGG TTTACAACTA GTAGAGAGTC AAAATGAATT ACAGAATCAA
    TTGGATAAAT GTCGTAATGA AATGATGACC ATGACTGAGA GTTATGAACA AGAAAAATAT
    ACCCTTCAAA GAGAAGTTGA ACTCAAGAGT CGAATGTTAG AAAGTTTGAG CTGCGAATGT
    GAAGCTATTA AACAACAACA AAAAATGCAC CTGGAGAAAT TGGAAGAACA ACTAAGCAGA
    AGCCATGGAC AGGAAGTGAA TGAACTAAAA ACTAAGATAG AAAAACTGAA AGTGGAATTA
    GATGAAGCCA GGCTTAGTGA AAAGCAGCTG AAGCACCAAG TAGATCATCA GAAGGAACTC
    CTCTCTTGTA AATCAGAGGA ACTGCGCGTA ATGTCTGAAC GTGTGCAGGA AAGCATGTCT
    TCAGAGATGC TGGCTCTTCA AATTGAGCTG ACAGAAATGG AGAGTATGAA GACCACCCTC
    AAAGAAGAAG TGAATGAACT ACAATACAGA CAAGAACAGC TAGAACTTCT TATTACTAAC
    CTAATGCGCC AGGTAGACCG GCTTAAAGAG GAAAAAGAGG AGCGAGAGAA AGAAGCAGTT
    TCTTACTATA ATGCCCTAGA GAAAGCTCGT GTAGCAAATC AAGATCTTCA GGTACAGTTG
    GACCAGGCAC TCCAGCAAGC CTTGGATCCC AATAGTAAAG GCAACTCTTT GTTTGCAGAG
    GTGGAAGATC GAAGGGCAGC AATGGAACGT CAGCTCATCA GTATGAAAGT CAAGTATCAG
    TCACTAAAGA AGCAAAATGT ATTTAACAGA GAACAGATGC AGAGAATGAA GTTACAAATT
    GCCACGTTGC TACAGATGAA AGGGTCTCAA ACTGAATTTG AGCAGCAGGA ACGGTTGCTT
    GCCATGTTGG AGCAGAAGAA TGGTGAAATA AAACATCTTT TAGGTGAAAT TAGAAATCTG
    GAGAAATTTA AGAATTTATA TGACAGTATG GAATCTAAGC CTTCAGTCGA CTCTGGTACT
    CTGGAAGATA ACACCTATTA TACAGATTTA CTTCAGATGA AGCTGGATAA CTTAAACAAA
    GAAATTGAAA GCACTAAAGG TGAATTGTCC ATACAGCGAA TGAAAGCATT ATTTGAGAGC
    CAGCGGGCTC TAGATATTGA GCGAAAACTT TTTGCAAATG AAAGATGCCT CCAGCTTTCA
    GAAAGTGAAA ATATGAAACT GAGAGCTAAA CTAGATGAAT TGAAACTAAA ATATGAACCT
    GAAGAGACAG TTGAAGTGCC TGTACTGAAA AAGAGGCGTG AGGTGCTCCC TGTGGATATA
    ACCACCGCTA AAGATGCATG TGTCAACAAC AGTGCTCTCG GGGGAGAAGT TTATCGATTA
    CCGCCTCAGA AAGAGGAGAC ACAGTCCTGC CCTAACAGTT TAGAAGATAA CAACTTGCAA
    TTAGAAAAAT CAGTTTCTAT ATACACACCA GTAGTCAGTC TCTCTCCTCA CAAAAATCTG
    CCCGTGGATA TGCAGCTGAA GAAGGAAAAG AAATGTGTGA AACTCATAGG AGTTCCCGCT
    GACGCTGAGG CCTTAAGTGA AAGAAGTGGA AACACCCCTA ACTCTCCCAG GTTAGCTGCT
    GAATCAAAGC TTCAAACAGA AGTTAAAGAA GGAAAAGAAA CTTCAAGCAA ATTGGAAAAA
    GAAACTTGTA AGAAATTACA CCCTATTCTA TATGTGTCTT CTAAATCTAC TCCAGAGACC
    CAGTGCCCTC AACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu68774
    Clone ID Related Accession (Same CDS sequence) NM_001329642.1 , NM_001329642.2
    Accession Version NM_001329642.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1656bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-30
    Organism Homo sapiens(human)
    Product protein Spindly isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008680.5 and BU616897.1. On Jul 21, 2016 this sequence version replaced XM_011534590.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.204765.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTTAGAAA GTTTGAGCTG CGAATGTGAA GCTATTAAAC AACAACAAAA AATGCACCTG 
    GAGAAATTGG AAGAACAACT AAGCAGAAGC CATGGACAGG AAGTGAATGA ACTAAAAACT
    AAGATAGAAA AACTGAAAGT GGAATTAGAT GAAGCCAGGC TTAGTGAAAA GCAGCTGAAG
    CACCAAGTAG ATCATCAGAA GGAACTCCTC TCTTGTAAAT CAGAGGAACT GCGCGTAATG
    TCTGAACGTG TGCAGGAAAG CATGTCTTCA GAGATGCTGG CTCTTCAAAT TGAGCTGACA
    GAAATGGAGA GTATGAAGAC CACCCTCAAA GAAGAAGTGA ATGAACTACA ATACAGACAA
    GAACAGCTAG AACTTCTTAT TACTAACCTA ATGCGCCAGG TAGACCGGCT TAAAGAGGAA
    AAAGAGGAGC GAGAGAAAGA AGCAGTTTCT TACTATAATG CCCTAGAGAA AGCTCGTGTA
    GCAAATCAAG ATCTTCAGGT ACAGTTGGAC CAGGCACTCC AGCAAGCCTT GGATCCCAAT
    AGTAAAGGCA ACTCTTTGTT TGCAGAGGTG GAAGATCGAA GGGCAGCAAT GGAACGTCAG
    CTCATCAGTA TGAAAGTCAA GTATCAGTCA CTAAAGAAGC AAAATGTATT TAACAGAGAA
    CAGATGCAGA GAATGAAGTT ACAAATTGCC ACGTTGCTAC AGATGAAAGG GTCTCAAACT
    GAATTTGAGC AGCAGGAACG GTTGCTTGCC ATGTTGGAGC AGAAGAATGG TGAAATAAAA
    CATCTTTTAG GTGAAATTAG AAATCTGGAG AAATTTAAGA ATTTATATGA CAGTATGGAA
    TCTAAGCCTT CAGTCGACTC TGGTACTCTG GAAGATAACA CCTATTATAC AGATTTACTT
    CAGATGAAGC TGGATAACTT AAACAAAGAA ATTGAAAGCA CTAAAGGTGA ATTGTCCATA
    CAGCGAATGA AAGCATTATT TGAGAGCCAG CGGGCTCTAG ATATTGAGCG AAAACTTTTT
    GCAAATGAAA GATGCCTCCA GCTTTCAGAA AGTGAAAATA TGAAACTGAG AGCTAAACTA
    GATGAATTGA AACTAAAATA TGAACCTGAA GAGACAGTTG AAGTGCCTGT ACTGAAAAAG
    AGGCGTGAGG TGCTCCCTGT GGATATAACC ACCGCTAAAG ATGCATGTGT CAACAACAGT
    GCTCTCGGGG GAGAAGTTTA TCGATTACCG CCTCAGAAAG AGGAGACACA GTCCTGCCCT
    AACAGTTTAG AAGATAACAA CTTGCAATTA GAAAAATCAG TTTCTATATA CACACCAGTA
    GTCAGTCTCT CTCCTCACAA AAATCTGCCC GTGGATATGC AGCTGAAGAA GGAAAAGAAA
    TGTGTGAAAC TCATAGGAGT TCCCGCTGAC GCTGAGGCCT TAAGTGAAAG AAGTGGAAAC
    ACCCCTAACT CTCCCAGGTC AGTGTCCTCT TTTCCTCCAG GCAGCCAGCA GACCTCTCCA
    TCTCTCCTCT CTCGCTGCAT GAACTGTGCT GTCTGTTTCT TTATCTACTT TCTTACAATT
    GCATGCAGTA TAATTCCTCA GTTTCATCTA CCTACCTTCA ACTTTTCCAG AACTTTAAGA
    AAGACTAAAC TGATTGCAAA GGGAAAGGAC TCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001316571.1
    CDS567..2222
    Translation

    Target ORF information:

    RefSeq Version NM_001329642.1
    Organism Homo sapiens(human)
    Definition Homo sapiens spindle apparatus coiled-coil protein 1 (SPDL1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001329642.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTTAGAAA GTTTGAGCTG CGAATGTGAA GCTATTAAAC AACAACAAAA AATGCACCTG 
    GAGAAATTGG AAGAACAACT AAGCAGAAGC CATGGACAGG AAGTGAATGA ACTAAAAACT
    AAGATAGAAA AACTGAAAGT GGAATTAGAT GAAGCCAGGC TTAGTGAAAA GCAGCTGAAG
    CACCAAGTAG ATCATCAGAA GGAACTCCTC TCTTGTAAAT CAGAGGAACT GCGCGTAATG
    TCTGAACGTG TGCAGGAAAG CATGTCTTCA GAGATGCTGG CTCTTCAAAT TGAGCTGACA
    GAAATGGAGA GTATGAAGAC CACCCTCAAA GAAGAAGTGA ATGAACTACA ATACAGACAA
    GAACAGCTAG AACTTCTTAT TACTAACCTA ATGCGCCAGG TAGACCGGCT TAAAGAGGAA
    AAAGAGGAGC GAGAGAAAGA AGCAGTTTCT TACTATAATG CCCTAGAGAA AGCTCGTGTA
    GCAAATCAAG ATCTTCAGGT ACAGTTGGAC CAGGCACTCC AGCAAGCCTT GGATCCCAAT
    AGTAAAGGCA ACTCTTTGTT TGCAGAGGTG GAAGATCGAA GGGCAGCAAT GGAACGTCAG
    CTCATCAGTA TGAAAGTCAA GTATCAGTCA CTAAAGAAGC AAAATGTATT TAACAGAGAA
    CAGATGCAGA GAATGAAGTT ACAAATTGCC ACGTTGCTAC AGATGAAAGG GTCTCAAACT
    GAATTTGAGC AGCAGGAACG GTTGCTTGCC ATGTTGGAGC AGAAGAATGG TGAAATAAAA
    CATCTTTTAG GTGAAATTAG AAATCTGGAG AAATTTAAGA ATTTATATGA CAGTATGGAA
    TCTAAGCCTT CAGTCGACTC TGGTACTCTG GAAGATAACA CCTATTATAC AGATTTACTT
    CAGATGAAGC TGGATAACTT AAACAAAGAA ATTGAAAGCA CTAAAGGTGA ATTGTCCATA
    CAGCGAATGA AAGCATTATT TGAGAGCCAG CGGGCTCTAG ATATTGAGCG AAAACTTTTT
    GCAAATGAAA GATGCCTCCA GCTTTCAGAA AGTGAAAATA TGAAACTGAG AGCTAAACTA
    GATGAATTGA AACTAAAATA TGAACCTGAA GAGACAGTTG AAGTGCCTGT ACTGAAAAAG
    AGGCGTGAGG TGCTCCCTGT GGATATAACC ACCGCTAAAG ATGCATGTGT CAACAACAGT
    GCTCTCGGGG GAGAAGTTTA TCGATTACCG CCTCAGAAAG AGGAGACACA GTCCTGCCCT
    AACAGTTTAG AAGATAACAA CTTGCAATTA GAAAAATCAG TTTCTATATA CACACCAGTA
    GTCAGTCTCT CTCCTCACAA AAATCTGCCC GTGGATATGC AGCTGAAGAA GGAAAAGAAA
    TGTGTGAAAC TCATAGGAGT TCCCGCTGAC GCTGAGGCCT TAAGTGAAAG AAGTGGAAAC
    ACCCCTAACT CTCCCAGGTC AGTGTCCTCT TTTCCTCCAG GCAGCCAGCA GACCTCTCCA
    TCTCTCCTCT CTCGCTGCAT GAACTGTGCT GTCTGTTTCT TTATCTACTT TCTTACAATT
    GCATGCAGTA TAATTCCTCA GTTTCATCTA CCTACCTTCA ACTTTTCCAG AACTTTAAGA
    AAGACTAAAC TGATTGCAAA GGGAAAGGAC TCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu68774
    Clone ID Related Accession (Same CDS sequence) NM_001329642.1 , NM_001329642.2
    Accession Version NM_001329642.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1656bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product protein Spindly isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008680.5 and BU616897.1. On May 31, 2019 this sequence version replaced NM_001329642.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.204765.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTTAGAAA GTTTGAGCTG CGAATGTGAA GCTATTAAAC AACAACAAAA AATGCACCTG 
    GAGAAATTGG AAGAACAACT AAGCAGAAGC CATGGACAGG AAGTGAATGA ACTAAAAACT
    AAGATAGAAA AACTGAAAGT GGAATTAGAT GAAGCCAGGC TTAGTGAAAA GCAGCTGAAG
    CACCAAGTAG ATCATCAGAA GGAACTCCTC TCTTGTAAAT CAGAGGAACT GCGCGTAATG
    TCTGAACGTG TGCAGGAAAG CATGTCTTCA GAGATGCTGG CTCTTCAAAT TGAGCTGACA
    GAAATGGAGA GTATGAAGAC CACCCTCAAA GAAGAAGTGA ATGAACTACA ATACAGACAA
    GAACAGCTAG AACTTCTTAT TACTAACCTA ATGCGCCAGG TAGACCGGCT TAAAGAGGAA
    AAAGAGGAGC GAGAGAAAGA AGCAGTTTCT TACTATAATG CCCTAGAGAA AGCTCGTGTA
    GCAAATCAAG ATCTTCAGGT ACAGTTGGAC CAGGCACTCC AGCAAGCCTT GGATCCCAAT
    AGTAAAGGCA ACTCTTTGTT TGCAGAGGTG GAAGATCGAA GGGCAGCAAT GGAACGTCAG
    CTCATCAGTA TGAAAGTCAA GTATCAGTCA CTAAAGAAGC AAAATGTATT TAACAGAGAA
    CAGATGCAGA GAATGAAGTT ACAAATTGCC ACGTTGCTAC AGATGAAAGG GTCTCAAACT
    GAATTTGAGC AGCAGGAACG GTTGCTTGCC ATGTTGGAGC AGAAGAATGG TGAAATAAAA
    CATCTTTTAG GTGAAATTAG AAATCTGGAG AAATTTAAGA ATTTATATGA CAGTATGGAA
    TCTAAGCCTT CAGTCGACTC TGGTACTCTG GAAGATAACA CCTATTATAC AGATTTACTT
    CAGATGAAGC TGGATAACTT AAACAAAGAA ATTGAAAGCA CTAAAGGTGA ATTGTCCATA
    CAGCGAATGA AAGCATTATT TGAGAGCCAG CGGGCTCTAG ATATTGAGCG AAAACTTTTT
    GCAAATGAAA GATGCCTCCA GCTTTCAGAA AGTGAAAATA TGAAACTGAG AGCTAAACTA
    GATGAATTGA AACTAAAATA TGAACCTGAA GAGACAGTTG AAGTGCCTGT ACTGAAAAAG
    AGGCGTGAGG TGCTCCCTGT GGATATAACC ACCGCTAAAG ATGCATGTGT CAACAACAGT
    GCTCTCGGGG GAGAAGTTTA TCGATTACCG CCTCAGAAAG AGGAGACACA GTCCTGCCCT
    AACAGTTTAG AAGATAACAA CTTGCAATTA GAAAAATCAG TTTCTATATA CACACCAGTA
    GTCAGTCTCT CTCCTCACAA AAATCTGCCC GTGGATATGC AGCTGAAGAA GGAAAAGAAA
    TGTGTGAAAC TCATAGGAGT TCCCGCTGAC GCTGAGGCCT TAAGTGAAAG AAGTGGAAAC
    ACCCCTAACT CTCCCAGGTC AGTGTCCTCT TTTCCTCCAG GCAGCCAGCA GACCTCTCCA
    TCTCTCCTCT CTCGCTGCAT GAACTGTGCT GTCTGTTTCT TTATCTACTT TCTTACAATT
    GCATGCAGTA TAATTCCTCA GTTTCATCTA CCTACCTTCA ACTTTTCCAG AACTTTAAGA
    AAGACTAAAC TGATTGCAAA GGGAAAGGAC TCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001316571.1
    CDS428..2083
    Translation

    Target ORF information:

    RefSeq Version NM_001329642.2
    Organism Homo sapiens(human)
    Definition Homo sapiens spindle apparatus coiled-coil protein 1 (SPDL1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001329642.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTTAGAAA GTTTGAGCTG CGAATGTGAA GCTATTAAAC AACAACAAAA AATGCACCTG 
    GAGAAATTGG AAGAACAACT AAGCAGAAGC CATGGACAGG AAGTGAATGA ACTAAAAACT
    AAGATAGAAA AACTGAAAGT GGAATTAGAT GAAGCCAGGC TTAGTGAAAA GCAGCTGAAG
    CACCAAGTAG ATCATCAGAA GGAACTCCTC TCTTGTAAAT CAGAGGAACT GCGCGTAATG
    TCTGAACGTG TGCAGGAAAG CATGTCTTCA GAGATGCTGG CTCTTCAAAT TGAGCTGACA
    GAAATGGAGA GTATGAAGAC CACCCTCAAA GAAGAAGTGA ATGAACTACA ATACAGACAA
    GAACAGCTAG AACTTCTTAT TACTAACCTA ATGCGCCAGG TAGACCGGCT TAAAGAGGAA
    AAAGAGGAGC GAGAGAAAGA AGCAGTTTCT TACTATAATG CCCTAGAGAA AGCTCGTGTA
    GCAAATCAAG ATCTTCAGGT ACAGTTGGAC CAGGCACTCC AGCAAGCCTT GGATCCCAAT
    AGTAAAGGCA ACTCTTTGTT TGCAGAGGTG GAAGATCGAA GGGCAGCAAT GGAACGTCAG
    CTCATCAGTA TGAAAGTCAA GTATCAGTCA CTAAAGAAGC AAAATGTATT TAACAGAGAA
    CAGATGCAGA GAATGAAGTT ACAAATTGCC ACGTTGCTAC AGATGAAAGG GTCTCAAACT
    GAATTTGAGC AGCAGGAACG GTTGCTTGCC ATGTTGGAGC AGAAGAATGG TGAAATAAAA
    CATCTTTTAG GTGAAATTAG AAATCTGGAG AAATTTAAGA ATTTATATGA CAGTATGGAA
    TCTAAGCCTT CAGTCGACTC TGGTACTCTG GAAGATAACA CCTATTATAC AGATTTACTT
    CAGATGAAGC TGGATAACTT AAACAAAGAA ATTGAAAGCA CTAAAGGTGA ATTGTCCATA
    CAGCGAATGA AAGCATTATT TGAGAGCCAG CGGGCTCTAG ATATTGAGCG AAAACTTTTT
    GCAAATGAAA GATGCCTCCA GCTTTCAGAA AGTGAAAATA TGAAACTGAG AGCTAAACTA
    GATGAATTGA AACTAAAATA TGAACCTGAA GAGACAGTTG AAGTGCCTGT ACTGAAAAAG
    AGGCGTGAGG TGCTCCCTGT GGATATAACC ACCGCTAAAG ATGCATGTGT CAACAACAGT
    GCTCTCGGGG GAGAAGTTTA TCGATTACCG CCTCAGAAAG AGGAGACACA GTCCTGCCCT
    AACAGTTTAG AAGATAACAA CTTGCAATTA GAAAAATCAG TTTCTATATA CACACCAGTA
    GTCAGTCTCT CTCCTCACAA AAATCTGCCC GTGGATATGC AGCTGAAGAA GGAAAAGAAA
    TGTGTGAAAC TCATAGGAGT TCCCGCTGAC GCTGAGGCCT TAAGTGAAAG AAGTGGAAAC
    ACCCCTAACT CTCCCAGGTC AGTGTCCTCT TTTCCTCCAG GCAGCCAGCA GACCTCTCCA
    TCTCTCCTCT CTCGCTGCAT GAACTGTGCT GTCTGTTTCT TTATCTACTT TCTTACAATT
    GCATGCAGTA TAATTCCTCA GTTTCATCTA CCTACCTTCA ACTTTTCCAG AACTTTAAGA
    AAGACTAAAC TGATTGCAAA GGGAAAGGAC TCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu101265
    Clone ID Related Accession (Same CDS sequence) NM_001329643.1 , NM_001329643.2
    Accession Version NM_001329643.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1605bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-30
    Organism Homo sapiens(human)
    Product protein Spindly isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008680.5 and BU616897.1. On Jul 21, 2016 this sequence version replaced XM_017009602.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.214818.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTTAGAAA GTTTGAGCTG CGAATGTGAA GCTATTAAAC AACAACAAAA AATGCACCTG 
    GAGAAATTGG AAGAACAACT AAGCAGAAGC CATGGACAGG AAGTGAATGA ACTAAAAACT
    AAGATAGAAA AACTGAAAGT GGAATTAGAT GAAGCCAGGC TTAGTGAAAA GCAGCTGAAG
    CACCAAGTAG ATCATCAGAA GGAACTCCTC TCTTGTAAAT CAGAGGAACT GCGCGTAATG
    TCTGAACGTG TGCAGGAAAG CATGTCTTCA GAGATGCTGG CTCTTCAAAT TGAGCTGACA
    GAAATGGAGA GTATGAAGAC CACCCTCAAA GAAGAAGTGA ATGAACTACA ATACAGACAA
    GAACAGCTAG AACTTCTTAT TACTAACCTA ATGCGCCAGG TAGACCGGCT TAAAGAGGAA
    AAAGAGGAGC GAGAGAAAGA AGCAGTTTCT TACTATAATG CCCTAGAGAA AGCTCGTGTA
    GCAAATCAAG ATCTTCAGGT ACAGTTGGAC CAGGCACTCC AGCAAGCCTT GGATCCCAAT
    AGTAAAGGCA ACTCTTTGTT TGCAGAGGTG GAAGATCGAA GGGCAGCAAT GGAACGTCAG
    CTCATCAGTA TGAAAGTCAA GTATCAGTCA CTAAAGAAGC AAAATGTATT TAACAGAGAA
    CAGATGCAGA GAATGAAGTT ACAAATTGCC ACGTTGCTAC AGATGAAAGG GTCTCAAACT
    GAATTTGAGC AGCAGGAACG GTTGCTTGCC ATGTTGGAGC AGAAGAATGG TGAAATAAAA
    CATCTTTTAG GTGAAATTAG AAATCTGGAG AAATTTAAGA ATTTATATGA CAGTATGGAA
    TCTAAGCCTT CAGTCGACTC TGGTACTCTG GAAGATAACA CCTATTATAC AGATTTACTT
    CAGATGAAGC TGGATAACTT AAACAAAGAA ATTGAAAGCA CTAAAGGTGA ATTGTCCATA
    CAGCGAATGA AAGCATTATT TGAGAGCCAG CGGGCTCTAG ATATTGAGCG AAAACTTTTT
    GCAAATGAAA GATGCCTCCA GCTTTCAGAA AGTGAAAATA TGAAACTGAG AGCTAAACTA
    GATGAATTGA AACTAAAATA TGAACCTGAA GAGACAGTTG AAGTGCCTGT ACTGAAAAAG
    AGGCGTGAGG TGCTCCCTGT GGATATAACC ACCGCTAAAG ATGCATGTGT CAACAACAGT
    GCTCTCGGGG GAGAAGTTTA TCGATTACCG CCTCAGAAAG AGGAGACACA GTCCTGCCCT
    AACAGTTTAG AAGATAACAA CTTGCAATTA GAAAAATCAG TTTCTATATA CACACCAGTA
    GTCAGTCTCT CTCCTCACAA AAATCTGCCC GTGGATATGC AGCTGAAGAA GGAAAAGAAA
    TGTGTGAAAC TCATAGGAGT TCCCGCTGAC GCTGAGGCCT TAAGTGAAAG AAGTGGAAAC
    ACCCCTAACT CTCCCAGGTT AGCTGCTGAA TCAAAGCTTC AAACAGAAGT TAAAGAAGGA
    AAAGAAACTT CAAGCAAATT GGAAAAAGAA ACTTGTAAGA AATTACACCC TATTCTATAT
    GTGTCTTCTA AATCTACTCC AGAGACCCAG TGCCCTCAAC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001316572.1
    CDS570..2174
    Translation

    Target ORF information:

    RefSeq Version NM_001329643.1
    Organism Homo sapiens(human)
    Definition Homo sapiens spindle apparatus coiled-coil protein 1 (SPDL1), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001329643.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTTAGAAA GTTTGAGCTG CGAATGTGAA GCTATTAAAC AACAACAAAA AATGCACCTG 
    GAGAAATTGG AAGAACAACT AAGCAGAAGC CATGGACAGG AAGTGAATGA ACTAAAAACT
    AAGATAGAAA AACTGAAAGT GGAATTAGAT GAAGCCAGGC TTAGTGAAAA GCAGCTGAAG
    CACCAAGTAG ATCATCAGAA GGAACTCCTC TCTTGTAAAT CAGAGGAACT GCGCGTAATG
    TCTGAACGTG TGCAGGAAAG CATGTCTTCA GAGATGCTGG CTCTTCAAAT TGAGCTGACA
    GAAATGGAGA GTATGAAGAC CACCCTCAAA GAAGAAGTGA ATGAACTACA ATACAGACAA
    GAACAGCTAG AACTTCTTAT TACTAACCTA ATGCGCCAGG TAGACCGGCT TAAAGAGGAA
    AAAGAGGAGC GAGAGAAAGA AGCAGTTTCT TACTATAATG CCCTAGAGAA AGCTCGTGTA
    GCAAATCAAG ATCTTCAGGT ACAGTTGGAC CAGGCACTCC AGCAAGCCTT GGATCCCAAT
    AGTAAAGGCA ACTCTTTGTT TGCAGAGGTG GAAGATCGAA GGGCAGCAAT GGAACGTCAG
    CTCATCAGTA TGAAAGTCAA GTATCAGTCA CTAAAGAAGC AAAATGTATT TAACAGAGAA
    CAGATGCAGA GAATGAAGTT ACAAATTGCC ACGTTGCTAC AGATGAAAGG GTCTCAAACT
    GAATTTGAGC AGCAGGAACG GTTGCTTGCC ATGTTGGAGC AGAAGAATGG TGAAATAAAA
    CATCTTTTAG GTGAAATTAG AAATCTGGAG AAATTTAAGA ATTTATATGA CAGTATGGAA
    TCTAAGCCTT CAGTCGACTC TGGTACTCTG GAAGATAACA CCTATTATAC AGATTTACTT
    CAGATGAAGC TGGATAACTT AAACAAAGAA ATTGAAAGCA CTAAAGGTGA ATTGTCCATA
    CAGCGAATGA AAGCATTATT TGAGAGCCAG CGGGCTCTAG ATATTGAGCG AAAACTTTTT
    GCAAATGAAA GATGCCTCCA GCTTTCAGAA AGTGAAAATA TGAAACTGAG AGCTAAACTA
    GATGAATTGA AACTAAAATA TGAACCTGAA GAGACAGTTG AAGTGCCTGT ACTGAAAAAG
    AGGCGTGAGG TGCTCCCTGT GGATATAACC ACCGCTAAAG ATGCATGTGT CAACAACAGT
    GCTCTCGGGG GAGAAGTTTA TCGATTACCG CCTCAGAAAG AGGAGACACA GTCCTGCCCT
    AACAGTTTAG AAGATAACAA CTTGCAATTA GAAAAATCAG TTTCTATATA CACACCAGTA
    GTCAGTCTCT CTCCTCACAA AAATCTGCCC GTGGATATGC AGCTGAAGAA GGAAAAGAAA
    TGTGTGAAAC TCATAGGAGT TCCCGCTGAC GCTGAGGCCT TAAGTGAAAG AAGTGGAAAC
    ACCCCTAACT CTCCCAGGTT AGCTGCTGAA TCAAAGCTTC AAACAGAAGT TAAAGAAGGA
    AAAGAAACTT CAAGCAAATT GGAAAAAGAA ACTTGTAAGA AATTACACCC TATTCTATAT
    GTGTCTTCTA AATCTACTCC AGAGACCCAG TGCCCTCAAC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu101265
    Clone ID Related Accession (Same CDS sequence) NM_001329643.1 , NM_001329643.2
    Accession Version NM_001329643.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1605bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product protein Spindly isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008680.5 and BU616897.1. On May 31, 2019 this sequence version replaced NM_001329643.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.214818.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTTAGAAA GTTTGAGCTG CGAATGTGAA GCTATTAAAC AACAACAAAA AATGCACCTG 
    GAGAAATTGG AAGAACAACT AAGCAGAAGC CATGGACAGG AAGTGAATGA ACTAAAAACT
    AAGATAGAAA AACTGAAAGT GGAATTAGAT GAAGCCAGGC TTAGTGAAAA GCAGCTGAAG
    CACCAAGTAG ATCATCAGAA GGAACTCCTC TCTTGTAAAT CAGAGGAACT GCGCGTAATG
    TCTGAACGTG TGCAGGAAAG CATGTCTTCA GAGATGCTGG CTCTTCAAAT TGAGCTGACA
    GAAATGGAGA GTATGAAGAC CACCCTCAAA GAAGAAGTGA ATGAACTACA ATACAGACAA
    GAACAGCTAG AACTTCTTAT TACTAACCTA ATGCGCCAGG TAGACCGGCT TAAAGAGGAA
    AAAGAGGAGC GAGAGAAAGA AGCAGTTTCT TACTATAATG CCCTAGAGAA AGCTCGTGTA
    GCAAATCAAG ATCTTCAGGT ACAGTTGGAC CAGGCACTCC AGCAAGCCTT GGATCCCAAT
    AGTAAAGGCA ACTCTTTGTT TGCAGAGGTG GAAGATCGAA GGGCAGCAAT GGAACGTCAG
    CTCATCAGTA TGAAAGTCAA GTATCAGTCA CTAAAGAAGC AAAATGTATT TAACAGAGAA
    CAGATGCAGA GAATGAAGTT ACAAATTGCC ACGTTGCTAC AGATGAAAGG GTCTCAAACT
    GAATTTGAGC AGCAGGAACG GTTGCTTGCC ATGTTGGAGC AGAAGAATGG TGAAATAAAA
    CATCTTTTAG GTGAAATTAG AAATCTGGAG AAATTTAAGA ATTTATATGA CAGTATGGAA
    TCTAAGCCTT CAGTCGACTC TGGTACTCTG GAAGATAACA CCTATTATAC AGATTTACTT
    CAGATGAAGC TGGATAACTT AAACAAAGAA ATTGAAAGCA CTAAAGGTGA ATTGTCCATA
    CAGCGAATGA AAGCATTATT TGAGAGCCAG CGGGCTCTAG ATATTGAGCG AAAACTTTTT
    GCAAATGAAA GATGCCTCCA GCTTTCAGAA AGTGAAAATA TGAAACTGAG AGCTAAACTA
    GATGAATTGA AACTAAAATA TGAACCTGAA GAGACAGTTG AAGTGCCTGT ACTGAAAAAG
    AGGCGTGAGG TGCTCCCTGT GGATATAACC ACCGCTAAAG ATGCATGTGT CAACAACAGT
    GCTCTCGGGG GAGAAGTTTA TCGATTACCG CCTCAGAAAG AGGAGACACA GTCCTGCCCT
    AACAGTTTAG AAGATAACAA CTTGCAATTA GAAAAATCAG TTTCTATATA CACACCAGTA
    GTCAGTCTCT CTCCTCACAA AAATCTGCCC GTGGATATGC AGCTGAAGAA GGAAAAGAAA
    TGTGTGAAAC TCATAGGAGT TCCCGCTGAC GCTGAGGCCT TAAGTGAAAG AAGTGGAAAC
    ACCCCTAACT CTCCCAGGTT AGCTGCTGAA TCAAAGCTTC AAACAGAAGT TAAAGAAGGA
    AAAGAAACTT CAAGCAAATT GGAAAAAGAA ACTTGTAAGA AATTACACCC TATTCTATAT
    GTGTCTTCTA AATCTACTCC AGAGACCCAG TGCCCTCAAC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001316572.1
    CDS431..2035
    Translation

    Target ORF information:

    RefSeq Version NM_001329643.2
    Organism Homo sapiens(human)
    Definition Homo sapiens spindle apparatus coiled-coil protein 1 (SPDL1), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001329643.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTTAGAAA GTTTGAGCTG CGAATGTGAA GCTATTAAAC AACAACAAAA AATGCACCTG 
    GAGAAATTGG AAGAACAACT AAGCAGAAGC CATGGACAGG AAGTGAATGA ACTAAAAACT
    AAGATAGAAA AACTGAAAGT GGAATTAGAT GAAGCCAGGC TTAGTGAAAA GCAGCTGAAG
    CACCAAGTAG ATCATCAGAA GGAACTCCTC TCTTGTAAAT CAGAGGAACT GCGCGTAATG
    TCTGAACGTG TGCAGGAAAG CATGTCTTCA GAGATGCTGG CTCTTCAAAT TGAGCTGACA
    GAAATGGAGA GTATGAAGAC CACCCTCAAA GAAGAAGTGA ATGAACTACA ATACAGACAA
    GAACAGCTAG AACTTCTTAT TACTAACCTA ATGCGCCAGG TAGACCGGCT TAAAGAGGAA
    AAAGAGGAGC GAGAGAAAGA AGCAGTTTCT TACTATAATG CCCTAGAGAA AGCTCGTGTA
    GCAAATCAAG ATCTTCAGGT ACAGTTGGAC CAGGCACTCC AGCAAGCCTT GGATCCCAAT
    AGTAAAGGCA ACTCTTTGTT TGCAGAGGTG GAAGATCGAA GGGCAGCAAT GGAACGTCAG
    CTCATCAGTA TGAAAGTCAA GTATCAGTCA CTAAAGAAGC AAAATGTATT TAACAGAGAA
    CAGATGCAGA GAATGAAGTT ACAAATTGCC ACGTTGCTAC AGATGAAAGG GTCTCAAACT
    GAATTTGAGC AGCAGGAACG GTTGCTTGCC ATGTTGGAGC AGAAGAATGG TGAAATAAAA
    CATCTTTTAG GTGAAATTAG AAATCTGGAG AAATTTAAGA ATTTATATGA CAGTATGGAA
    TCTAAGCCTT CAGTCGACTC TGGTACTCTG GAAGATAACA CCTATTATAC AGATTTACTT
    CAGATGAAGC TGGATAACTT AAACAAAGAA ATTGAAAGCA CTAAAGGTGA ATTGTCCATA
    CAGCGAATGA AAGCATTATT TGAGAGCCAG CGGGCTCTAG ATATTGAGCG AAAACTTTTT
    GCAAATGAAA GATGCCTCCA GCTTTCAGAA AGTGAAAATA TGAAACTGAG AGCTAAACTA
    GATGAATTGA AACTAAAATA TGAACCTGAA GAGACAGTTG AAGTGCCTGT ACTGAAAAAG
    AGGCGTGAGG TGCTCCCTGT GGATATAACC ACCGCTAAAG ATGCATGTGT CAACAACAGT
    GCTCTCGGGG GAGAAGTTTA TCGATTACCG CCTCAGAAAG AGGAGACACA GTCCTGCCCT
    AACAGTTTAG AAGATAACAA CTTGCAATTA GAAAAATCAG TTTCTATATA CACACCAGTA
    GTCAGTCTCT CTCCTCACAA AAATCTGCCC GTGGATATGC AGCTGAAGAA GGAAAAGAAA
    TGTGTGAAAC TCATAGGAGT TCCCGCTGAC GCTGAGGCCT TAAGTGAAAG AAGTGGAAAC
    ACCCCTAACT CTCCCAGGTT AGCTGCTGAA TCAAAGCTTC AAACAGAAGT TAAAGAAGGA
    AAAGAAACTT CAAGCAAATT GGAAAAAGAA ACTTGTAAGA AATTACACCC TATTCTATAT
    GTGTCTTCTA AATCTACTCC AGAGACCCAG TGCCCTCAAC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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