RFWD3 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following RFWD3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RFWD3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu02691 NM_018124.4
Latest version!
Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu108103 NM_001370543.1
Latest version!
Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu108103 NM_001370537.1
Latest version!
Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu108103 NM_001370542.1
Latest version!
Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu108103 NM_001370540.1
Latest version!
Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu108103 NM_001370539.1
Latest version!
Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu02691 NM_001370534.1
Latest version!
Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $265.30
$379.00
OHu02691 NM_001370535.1
Latest version!
Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $265.30
$379.00
OHu49413 NM_001370536.1
Latest version!
Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu02691 XM_011523191.3
Latest version!
Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $265.30
$379.00
OHu02691 XM_005256021.4
Latest version!
Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $265.30
$379.00
OHu02691 XM_017023391.1
Latest version!
Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $265.30
$379.00
OHu02691 XM_005256022.4
Latest version!
Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $265.30
$379.00
OHu49413 XM_006721228.3
Latest version!
Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu49413 XM_017023392.1
Latest version!
Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
View Old Accession Versions >>
OHu02691 NM_018124.3 Homo sapiens ring finger and WD repeat domain 3 (RFWD3), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $265.30
$379.00
OHu02691 XM_011523191.2 Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $265.30
$379.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu02691
    Clone ID Related Accession (Same CDS sequence) NM_018124.3 , NM_001370534.1 , XM_017023391.1 , NM_018124.4 , XM_011523191.2 , XM_011523191.3 , XM_005256021.4 , NM_001370535.1 , XM_005256022.4
    Accession Version NM_018124.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2325bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-06
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase RFWD3 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BP872110.1, BC059371.1, BC002574.2 and BM467919.1. On Nov 22, 2018 this sequence version replaced NM_018124.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.21672.1, SRR1660805.195981.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000361070.9/ ENSP00000354361.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
    CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT
    GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG
    ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG
    ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA
    CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG
    ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA
    TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG
    GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT
    TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT
    AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC
    TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA
    GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC
    CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT
    GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC
    CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT
    AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC
    GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT
    TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA
    CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG
    CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG
    GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG
    ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC
    TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG
    ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC
    CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC
    ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT
    CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC
    AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA
    GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT
    GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG
    AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC
    TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC
    ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA
    ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC
    AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT
    GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA
    CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG
    GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_060594.3
    CDS95..2419
    Translation

    Target ORF information:

    RefSeq Version NM_018124.4
    Organism Homo sapiens(human)
    Definition Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_018124.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
    CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT
    GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG
    ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG
    ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA
    CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG
    ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA
    TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG
    GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT
    TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT
    AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC
    TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA
    GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC
    CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT
    GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC
    CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT
    AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC
    GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT
    TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA
    CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG
    CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG
    GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG
    ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC
    TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG
    ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC
    CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC
    ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT
    CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC
    AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA
    GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT
    GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG
    AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC
    TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC
    ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA
    ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC
    AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT
    GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA
    CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG
    GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu108103
    Clone ID Related Accession (Same CDS sequence) NM_001370537.1 , NM_001370539.1 , NM_001370540.1 , NM_001370543.1 , NM_001370542.1
    Accession Version NM_001370537.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1491bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-06
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase RFWD3 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC109599.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.4859.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGATGAGG AAGAAGGGGA CACTTGTACA ATATGTCTGG AACAGTGGAC CAATGCTGGG 
    GACCACCGGC TCTCAGCATT ACGCTGTGGG CATCTCTTTG GGTATAGGTG CATTTCCACG
    TGGCTTAAAG GACAAGTACG AAAATGTCCC CAGTGCAACA AGAAAGCCAG GCACAGTGAC
    ATTGTCGTCC TTTATGCCCG AACCCTGAGA GCTTTGGACA CTAGTGAACA GGAGCGCATG
    AAAAGTTCCC TACTGAAGGA ACAGATGCTA AGGAAACAGG CCGAGTTAGA ATCAGCACAG
    TGCCGACTCC AACTGCAGGT CCTCACTGAT AAGTGCACTA GGCTTCAAAG GCGTGTTCAG
    GACTTGCAAA AACTTACGTC ACATCAAAGT CAGAATTTAC AGCAACCCAG GGGCTCCCAA
    GCATGGGTCC TGAGCTGCTC ACCCTCCAGC CAGGGCCAGC ACAAGCACAA GTACCACTTC
    CAAAAGACCT TCACAGTATC TCAGGCAGGA AACTGCCGGA TCATGGCATA CTGTGATGCT
    CTGAGCTGCC TGGTGATATC ACAGCCTTCT CCTCAGGCCT CTTTTCTTCC AGGCTTTGGT
    GTTAAGATGT TGAGTACTGC CAACATGAAG AGCAGTCAGT ACATTCCGAT GCATGGCAAA
    CAGATCCGTG GACTGGCGTT TAGCAGTTAC CTCAGAGGCT TGCTACTCTC TGCTTCCCTA
    GACAACACTA TTAAACTGAC CAGCCTGGAG ACAAATACCG TGGTCCAGAC TTATAATGCT
    GGACGTCCTG TCTGGAGCTG TTGCTGGTGT CTTGATGAGG CTAACTACAT CTATGCTGGA
    CTGGCCAATG GTTCAATTCT GGTATATGAC GTGCGAAACA CGAGCAGTCA TGTGCAGGAG
    TTAGTAGCTC AGAAAGCCAG ATGCCCACTG GTCTCCCTGT CATACATGCC CAGAGCTGCC
    TCAGCTGCAT TTCCATATGG TGGGGTGCTG GCTGGAACCT TGGAGGATGC TTCATTCTGG
    GAACAGAAAA TGGACTTTTC TCATTGGCCT CATGTGCTGC CCTTGGAGCC AGGGGGCTGC
    ATAGACTTTC AGACAGAGAA CAGCTCCCGG CACTGTCTTG TGACCTACAG GCCTGATAAA
    AATCACACCA CCATACGAAG TGTGCTGATG GAAATGTCCT ACCGACTGGA TGACACTGGA
    AATCCAATCT GCTCCTGCCA GCCTGTACAT ACATTTTTTG GAGGACCTAC TTGCAAACTA
    TTGACCAAAA ATGCCATTTT CCAAAGCCCA GAGAATGATG GCAACATCCT GGTGTGTACT
    GGGGATGAAG CAGCAAATTC TGCCCTGCTG TGGGATGCTG CCAGTGGCTC GTTGCTCCAG
    GACCTACAGA CCGATCAGCC TGTGTTGGAC ATCTGCCCAT TTGAGGTGAA CCGTAACAGC
    TACTTGGCTA CCTTAACAGA GAAGATGGTC CACATCTATA AGTGGGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001357466.1
    CDS1103..2593
    Translation

    Target ORF information:

    RefSeq Version NM_001370537.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001370537.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGATGAGG AAGAAGGGGA CACTTGTACA ATATGTCTGG AACAGTGGAC CAATGCTGGG 
    GACCACCGGC TCTCAGCATT ACGCTGTGGG CATCTCTTTG GGTATAGGTG CATTTCCACG
    TGGCTTAAAG GACAAGTACG AAAATGTCCC CAGTGCAACA AGAAAGCCAG GCACAGTGAC
    ATTGTCGTCC TTTATGCCCG AACCCTGAGA GCTTTGGACA CTAGTGAACA GGAGCGCATG
    AAAAGTTCCC TACTGAAGGA ACAGATGCTA AGGAAACAGG CCGAGTTAGA ATCAGCACAG
    TGCCGACTCC AACTGCAGGT CCTCACTGAT AAGTGCACTA GGCTTCAAAG GCGTGTTCAG
    GACTTGCAAA AACTTACGTC ACATCAAAGT CAGAATTTAC AGCAACCCAG GGGCTCCCAA
    GCATGGGTCC TGAGCTGCTC ACCCTCCAGC CAGGGCCAGC ACAAGCACAA GTACCACTTC
    CAAAAGACCT TCACAGTATC TCAGGCAGGA AACTGCCGGA TCATGGCATA CTGTGATGCT
    CTGAGCTGCC TGGTGATATC ACAGCCTTCT CCTCAGGCCT CTTTTCTTCC AGGCTTTGGT
    GTTAAGATGT TGAGTACTGC CAACATGAAG AGCAGTCAGT ACATTCCGAT GCATGGCAAA
    CAGATCCGTG GACTGGCGTT TAGCAGTTAC CTCAGAGGCT TGCTACTCTC TGCTTCCCTA
    GACAACACTA TTAAACTGAC CAGCCTGGAG ACAAATACCG TGGTCCAGAC TTATAATGCT
    GGACGTCCTG TCTGGAGCTG TTGCTGGTGT CTTGATGAGG CTAACTACAT CTATGCTGGA
    CTGGCCAATG GTTCAATTCT GGTATATGAC GTGCGAAACA CGAGCAGTCA TGTGCAGGAG
    TTAGTAGCTC AGAAAGCCAG ATGCCCACTG GTCTCCCTGT CATACATGCC CAGAGCTGCC
    TCAGCTGCAT TTCCATATGG TGGGGTGCTG GCTGGAACCT TGGAGGATGC TTCATTCTGG
    GAACAGAAAA TGGACTTTTC TCATTGGCCT CATGTGCTGC CCTTGGAGCC AGGGGGCTGC
    ATAGACTTTC AGACAGAGAA CAGCTCCCGG CACTGTCTTG TGACCTACAG GCCTGATAAA
    AATCACACCA CCATACGAAG TGTGCTGATG GAAATGTCCT ACCGACTGGA TGACACTGGA
    AATCCAATCT GCTCCTGCCA GCCTGTACAT ACATTTTTTG GAGGACCTAC TTGCAAACTA
    TTGACCAAAA ATGCCATTTT CCAAAGCCCA GAGAATGATG GCAACATCCT GGTGTGTACT
    GGGGATGAAG CAGCAAATTC TGCCCTGCTG TGGGATGCTG CCAGTGGCTC GTTGCTCCAG
    GACCTACAGA CCGATCAGCC TGTGTTGGAC ATCTGCCCAT TTGAGGTGAA CCGTAACAGC
    TACTTGGCTA CCTTAACAGA GAAGATGGTC CACATCTATA AGTGGGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu108103
    Clone ID Related Accession (Same CDS sequence) NM_001370537.1 , NM_001370539.1 , NM_001370540.1 , NM_001370543.1 , NM_001370542.1
    Accession Version NM_001370539.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1491bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase RFWD3 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC109599.4. On May 20, 2019 this sequence version replaced NM_001370541.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.91940.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGATGAGG AAGAAGGGGA CACTTGTACA ATATGTCTGG AACAGTGGAC CAATGCTGGG 
    GACCACCGGC TCTCAGCATT ACGCTGTGGG CATCTCTTTG GGTATAGGTG CATTTCCACG
    TGGCTTAAAG GACAAGTACG AAAATGTCCC CAGTGCAACA AGAAAGCCAG GCACAGTGAC
    ATTGTCGTCC TTTATGCCCG AACCCTGAGA GCTTTGGACA CTAGTGAACA GGAGCGCATG
    AAAAGTTCCC TACTGAAGGA ACAGATGCTA AGGAAACAGG CCGAGTTAGA ATCAGCACAG
    TGCCGACTCC AACTGCAGGT CCTCACTGAT AAGTGCACTA GGCTTCAAAG GCGTGTTCAG
    GACTTGCAAA AACTTACGTC ACATCAAAGT CAGAATTTAC AGCAACCCAG GGGCTCCCAA
    GCATGGGTCC TGAGCTGCTC ACCCTCCAGC CAGGGCCAGC ACAAGCACAA GTACCACTTC
    CAAAAGACCT TCACAGTATC TCAGGCAGGA AACTGCCGGA TCATGGCATA CTGTGATGCT
    CTGAGCTGCC TGGTGATATC ACAGCCTTCT CCTCAGGCCT CTTTTCTTCC AGGCTTTGGT
    GTTAAGATGT TGAGTACTGC CAACATGAAG AGCAGTCAGT ACATTCCGAT GCATGGCAAA
    CAGATCCGTG GACTGGCGTT TAGCAGTTAC CTCAGAGGCT TGCTACTCTC TGCTTCCCTA
    GACAACACTA TTAAACTGAC CAGCCTGGAG ACAAATACCG TGGTCCAGAC TTATAATGCT
    GGACGTCCTG TCTGGAGCTG TTGCTGGTGT CTTGATGAGG CTAACTACAT CTATGCTGGA
    CTGGCCAATG GTTCAATTCT GGTATATGAC GTGCGAAACA CGAGCAGTCA TGTGCAGGAG
    TTAGTAGCTC AGAAAGCCAG ATGCCCACTG GTCTCCCTGT CATACATGCC CAGAGCTGCC
    TCAGCTGCAT TTCCATATGG TGGGGTGCTG GCTGGAACCT TGGAGGATGC TTCATTCTGG
    GAACAGAAAA TGGACTTTTC TCATTGGCCT CATGTGCTGC CCTTGGAGCC AGGGGGCTGC
    ATAGACTTTC AGACAGAGAA CAGCTCCCGG CACTGTCTTG TGACCTACAG GCCTGATAAA
    AATCACACCA CCATACGAAG TGTGCTGATG GAAATGTCCT ACCGACTGGA TGACACTGGA
    AATCCAATCT GCTCCTGCCA GCCTGTACAT ACATTTTTTG GAGGACCTAC TTGCAAACTA
    TTGACCAAAA ATGCCATTTT CCAAAGCCCA GAGAATGATG GCAACATCCT GGTGTGTACT
    GGGGATGAAG CAGCAAATTC TGCCCTGCTG TGGGATGCTG CCAGTGGCTC GTTGCTCCAG
    GACCTACAGA CCGATCAGCC TGTGTTGGAC ATCTGCCCAT TTGAGGTGAA CCGTAACAGC
    TACTTGGCTA CCTTAACAGA GAAGATGGTC CACATCTATA AGTGGGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001357468.1
    CDS726..2216
    Translation

    Target ORF information:

    RefSeq Version NM_001370539.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001370539.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGATGAGG AAGAAGGGGA CACTTGTACA ATATGTCTGG AACAGTGGAC CAATGCTGGG 
    GACCACCGGC TCTCAGCATT ACGCTGTGGG CATCTCTTTG GGTATAGGTG CATTTCCACG
    TGGCTTAAAG GACAAGTACG AAAATGTCCC CAGTGCAACA AGAAAGCCAG GCACAGTGAC
    ATTGTCGTCC TTTATGCCCG AACCCTGAGA GCTTTGGACA CTAGTGAACA GGAGCGCATG
    AAAAGTTCCC TACTGAAGGA ACAGATGCTA AGGAAACAGG CCGAGTTAGA ATCAGCACAG
    TGCCGACTCC AACTGCAGGT CCTCACTGAT AAGTGCACTA GGCTTCAAAG GCGTGTTCAG
    GACTTGCAAA AACTTACGTC ACATCAAAGT CAGAATTTAC AGCAACCCAG GGGCTCCCAA
    GCATGGGTCC TGAGCTGCTC ACCCTCCAGC CAGGGCCAGC ACAAGCACAA GTACCACTTC
    CAAAAGACCT TCACAGTATC TCAGGCAGGA AACTGCCGGA TCATGGCATA CTGTGATGCT
    CTGAGCTGCC TGGTGATATC ACAGCCTTCT CCTCAGGCCT CTTTTCTTCC AGGCTTTGGT
    GTTAAGATGT TGAGTACTGC CAACATGAAG AGCAGTCAGT ACATTCCGAT GCATGGCAAA
    CAGATCCGTG GACTGGCGTT TAGCAGTTAC CTCAGAGGCT TGCTACTCTC TGCTTCCCTA
    GACAACACTA TTAAACTGAC CAGCCTGGAG ACAAATACCG TGGTCCAGAC TTATAATGCT
    GGACGTCCTG TCTGGAGCTG TTGCTGGTGT CTTGATGAGG CTAACTACAT CTATGCTGGA
    CTGGCCAATG GTTCAATTCT GGTATATGAC GTGCGAAACA CGAGCAGTCA TGTGCAGGAG
    TTAGTAGCTC AGAAAGCCAG ATGCCCACTG GTCTCCCTGT CATACATGCC CAGAGCTGCC
    TCAGCTGCAT TTCCATATGG TGGGGTGCTG GCTGGAACCT TGGAGGATGC TTCATTCTGG
    GAACAGAAAA TGGACTTTTC TCATTGGCCT CATGTGCTGC CCTTGGAGCC AGGGGGCTGC
    ATAGACTTTC AGACAGAGAA CAGCTCCCGG CACTGTCTTG TGACCTACAG GCCTGATAAA
    AATCACACCA CCATACGAAG TGTGCTGATG GAAATGTCCT ACCGACTGGA TGACACTGGA
    AATCCAATCT GCTCCTGCCA GCCTGTACAT ACATTTTTTG GAGGACCTAC TTGCAAACTA
    TTGACCAAAA ATGCCATTTT CCAAAGCCCA GAGAATGATG GCAACATCCT GGTGTGTACT
    GGGGATGAAG CAGCAAATTC TGCCCTGCTG TGGGATGCTG CCAGTGGCTC GTTGCTCCAG
    GACCTACAGA CCGATCAGCC TGTGTTGGAC ATCTGCCCAT TTGAGGTGAA CCGTAACAGC
    TACTTGGCTA CCTTAACAGA GAAGATGGTC CACATCTATA AGTGGGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu108103
    Clone ID Related Accession (Same CDS sequence) NM_001370537.1 , NM_001370539.1 , NM_001370540.1 , NM_001370543.1 , NM_001370542.1
    Accession Version NM_001370540.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1491bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase RFWD3 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC109599.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.19411.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGATGAGG AAGAAGGGGA CACTTGTACA ATATGTCTGG AACAGTGGAC CAATGCTGGG 
    GACCACCGGC TCTCAGCATT ACGCTGTGGG CATCTCTTTG GGTATAGGTG CATTTCCACG
    TGGCTTAAAG GACAAGTACG AAAATGTCCC CAGTGCAACA AGAAAGCCAG GCACAGTGAC
    ATTGTCGTCC TTTATGCCCG AACCCTGAGA GCTTTGGACA CTAGTGAACA GGAGCGCATG
    AAAAGTTCCC TACTGAAGGA ACAGATGCTA AGGAAACAGG CCGAGTTAGA ATCAGCACAG
    TGCCGACTCC AACTGCAGGT CCTCACTGAT AAGTGCACTA GGCTTCAAAG GCGTGTTCAG
    GACTTGCAAA AACTTACGTC ACATCAAAGT CAGAATTTAC AGCAACCCAG GGGCTCCCAA
    GCATGGGTCC TGAGCTGCTC ACCCTCCAGC CAGGGCCAGC ACAAGCACAA GTACCACTTC
    CAAAAGACCT TCACAGTATC TCAGGCAGGA AACTGCCGGA TCATGGCATA CTGTGATGCT
    CTGAGCTGCC TGGTGATATC ACAGCCTTCT CCTCAGGCCT CTTTTCTTCC AGGCTTTGGT
    GTTAAGATGT TGAGTACTGC CAACATGAAG AGCAGTCAGT ACATTCCGAT GCATGGCAAA
    CAGATCCGTG GACTGGCGTT TAGCAGTTAC CTCAGAGGCT TGCTACTCTC TGCTTCCCTA
    GACAACACTA TTAAACTGAC CAGCCTGGAG ACAAATACCG TGGTCCAGAC TTATAATGCT
    GGACGTCCTG TCTGGAGCTG TTGCTGGTGT CTTGATGAGG CTAACTACAT CTATGCTGGA
    CTGGCCAATG GTTCAATTCT GGTATATGAC GTGCGAAACA CGAGCAGTCA TGTGCAGGAG
    TTAGTAGCTC AGAAAGCCAG ATGCCCACTG GTCTCCCTGT CATACATGCC CAGAGCTGCC
    TCAGCTGCAT TTCCATATGG TGGGGTGCTG GCTGGAACCT TGGAGGATGC TTCATTCTGG
    GAACAGAAAA TGGACTTTTC TCATTGGCCT CATGTGCTGC CCTTGGAGCC AGGGGGCTGC
    ATAGACTTTC AGACAGAGAA CAGCTCCCGG CACTGTCTTG TGACCTACAG GCCTGATAAA
    AATCACACCA CCATACGAAG TGTGCTGATG GAAATGTCCT ACCGACTGGA TGACACTGGA
    AATCCAATCT GCTCCTGCCA GCCTGTACAT ACATTTTTTG GAGGACCTAC TTGCAAACTA
    TTGACCAAAA ATGCCATTTT CCAAAGCCCA GAGAATGATG GCAACATCCT GGTGTGTACT
    GGGGATGAAG CAGCAAATTC TGCCCTGCTG TGGGATGCTG CCAGTGGCTC GTTGCTCCAG
    GACCTACAGA CCGATCAGCC TGTGTTGGAC ATCTGCCCAT TTGAGGTGAA CCGTAACAGC
    TACTTGGCTA CCTTAACAGA GAAGATGGTC CACATCTATA AGTGGGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001357469.1
    CDS579..2069
    Translation

    Target ORF information:

    RefSeq Version NM_001370540.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001370540.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGATGAGG AAGAAGGGGA CACTTGTACA ATATGTCTGG AACAGTGGAC CAATGCTGGG 
    GACCACCGGC TCTCAGCATT ACGCTGTGGG CATCTCTTTG GGTATAGGTG CATTTCCACG
    TGGCTTAAAG GACAAGTACG AAAATGTCCC CAGTGCAACA AGAAAGCCAG GCACAGTGAC
    ATTGTCGTCC TTTATGCCCG AACCCTGAGA GCTTTGGACA CTAGTGAACA GGAGCGCATG
    AAAAGTTCCC TACTGAAGGA ACAGATGCTA AGGAAACAGG CCGAGTTAGA ATCAGCACAG
    TGCCGACTCC AACTGCAGGT CCTCACTGAT AAGTGCACTA GGCTTCAAAG GCGTGTTCAG
    GACTTGCAAA AACTTACGTC ACATCAAAGT CAGAATTTAC AGCAACCCAG GGGCTCCCAA
    GCATGGGTCC TGAGCTGCTC ACCCTCCAGC CAGGGCCAGC ACAAGCACAA GTACCACTTC
    CAAAAGACCT TCACAGTATC TCAGGCAGGA AACTGCCGGA TCATGGCATA CTGTGATGCT
    CTGAGCTGCC TGGTGATATC ACAGCCTTCT CCTCAGGCCT CTTTTCTTCC AGGCTTTGGT
    GTTAAGATGT TGAGTACTGC CAACATGAAG AGCAGTCAGT ACATTCCGAT GCATGGCAAA
    CAGATCCGTG GACTGGCGTT TAGCAGTTAC CTCAGAGGCT TGCTACTCTC TGCTTCCCTA
    GACAACACTA TTAAACTGAC CAGCCTGGAG ACAAATACCG TGGTCCAGAC TTATAATGCT
    GGACGTCCTG TCTGGAGCTG TTGCTGGTGT CTTGATGAGG CTAACTACAT CTATGCTGGA
    CTGGCCAATG GTTCAATTCT GGTATATGAC GTGCGAAACA CGAGCAGTCA TGTGCAGGAG
    TTAGTAGCTC AGAAAGCCAG ATGCCCACTG GTCTCCCTGT CATACATGCC CAGAGCTGCC
    TCAGCTGCAT TTCCATATGG TGGGGTGCTG GCTGGAACCT TGGAGGATGC TTCATTCTGG
    GAACAGAAAA TGGACTTTTC TCATTGGCCT CATGTGCTGC CCTTGGAGCC AGGGGGCTGC
    ATAGACTTTC AGACAGAGAA CAGCTCCCGG CACTGTCTTG TGACCTACAG GCCTGATAAA
    AATCACACCA CCATACGAAG TGTGCTGATG GAAATGTCCT ACCGACTGGA TGACACTGGA
    AATCCAATCT GCTCCTGCCA GCCTGTACAT ACATTTTTTG GAGGACCTAC TTGCAAACTA
    TTGACCAAAA ATGCCATTTT CCAAAGCCCA GAGAATGATG GCAACATCCT GGTGTGTACT
    GGGGATGAAG CAGCAAATTC TGCCCTGCTG TGGGATGCTG CCAGTGGCTC GTTGCTCCAG
    GACCTACAGA CCGATCAGCC TGTGTTGGAC ATCTGCCCAT TTGAGGTGAA CCGTAACAGC
    TACTTGGCTA CCTTAACAGA GAAGATGGTC CACATCTATA AGTGGGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu108103
    Clone ID Related Accession (Same CDS sequence) NM_001370537.1 , NM_001370539.1 , NM_001370540.1 , NM_001370543.1 , NM_001370542.1
    Accession Version NM_001370543.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1491bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-06
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase RFWD3 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC109599.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.234889.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGATGAGG AAGAAGGGGA CACTTGTACA ATATGTCTGG AACAGTGGAC CAATGCTGGG 
    GACCACCGGC TCTCAGCATT ACGCTGTGGG CATCTCTTTG GGTATAGGTG CATTTCCACG
    TGGCTTAAAG GACAAGTACG AAAATGTCCC CAGTGCAACA AGAAAGCCAG GCACAGTGAC
    ATTGTCGTCC TTTATGCCCG AACCCTGAGA GCTTTGGACA CTAGTGAACA GGAGCGCATG
    AAAAGTTCCC TACTGAAGGA ACAGATGCTA AGGAAACAGG CCGAGTTAGA ATCAGCACAG
    TGCCGACTCC AACTGCAGGT CCTCACTGAT AAGTGCACTA GGCTTCAAAG GCGTGTTCAG
    GACTTGCAAA AACTTACGTC ACATCAAAGT CAGAATTTAC AGCAACCCAG GGGCTCCCAA
    GCATGGGTCC TGAGCTGCTC ACCCTCCAGC CAGGGCCAGC ACAAGCACAA GTACCACTTC
    CAAAAGACCT TCACAGTATC TCAGGCAGGA AACTGCCGGA TCATGGCATA CTGTGATGCT
    CTGAGCTGCC TGGTGATATC ACAGCCTTCT CCTCAGGCCT CTTTTCTTCC AGGCTTTGGT
    GTTAAGATGT TGAGTACTGC CAACATGAAG AGCAGTCAGT ACATTCCGAT GCATGGCAAA
    CAGATCCGTG GACTGGCGTT TAGCAGTTAC CTCAGAGGCT TGCTACTCTC TGCTTCCCTA
    GACAACACTA TTAAACTGAC CAGCCTGGAG ACAAATACCG TGGTCCAGAC TTATAATGCT
    GGACGTCCTG TCTGGAGCTG TTGCTGGTGT CTTGATGAGG CTAACTACAT CTATGCTGGA
    CTGGCCAATG GTTCAATTCT GGTATATGAC GTGCGAAACA CGAGCAGTCA TGTGCAGGAG
    TTAGTAGCTC AGAAAGCCAG ATGCCCACTG GTCTCCCTGT CATACATGCC CAGAGCTGCC
    TCAGCTGCAT TTCCATATGG TGGGGTGCTG GCTGGAACCT TGGAGGATGC TTCATTCTGG
    GAACAGAAAA TGGACTTTTC TCATTGGCCT CATGTGCTGC CCTTGGAGCC AGGGGGCTGC
    ATAGACTTTC AGACAGAGAA CAGCTCCCGG CACTGTCTTG TGACCTACAG GCCTGATAAA
    AATCACACCA CCATACGAAG TGTGCTGATG GAAATGTCCT ACCGACTGGA TGACACTGGA
    AATCCAATCT GCTCCTGCCA GCCTGTACAT ACATTTTTTG GAGGACCTAC TTGCAAACTA
    TTGACCAAAA ATGCCATTTT CCAAAGCCCA GAGAATGATG GCAACATCCT GGTGTGTACT
    GGGGATGAAG CAGCAAATTC TGCCCTGCTG TGGGATGCTG CCAGTGGCTC GTTGCTCCAG
    GACCTACAGA CCGATCAGCC TGTGTTGGAC ATCTGCCCAT TTGAGGTGAA CCGTAACAGC
    TACTTGGCTA CCTTAACAGA GAAGATGGTC CACATCTATA AGTGGGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001357472.1
    CDS980..2470
    Translation

    Target ORF information:

    RefSeq Version NM_001370543.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001370543.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGATGAGG AAGAAGGGGA CACTTGTACA ATATGTCTGG AACAGTGGAC CAATGCTGGG 
    GACCACCGGC TCTCAGCATT ACGCTGTGGG CATCTCTTTG GGTATAGGTG CATTTCCACG
    TGGCTTAAAG GACAAGTACG AAAATGTCCC CAGTGCAACA AGAAAGCCAG GCACAGTGAC
    ATTGTCGTCC TTTATGCCCG AACCCTGAGA GCTTTGGACA CTAGTGAACA GGAGCGCATG
    AAAAGTTCCC TACTGAAGGA ACAGATGCTA AGGAAACAGG CCGAGTTAGA ATCAGCACAG
    TGCCGACTCC AACTGCAGGT CCTCACTGAT AAGTGCACTA GGCTTCAAAG GCGTGTTCAG
    GACTTGCAAA AACTTACGTC ACATCAAAGT CAGAATTTAC AGCAACCCAG GGGCTCCCAA
    GCATGGGTCC TGAGCTGCTC ACCCTCCAGC CAGGGCCAGC ACAAGCACAA GTACCACTTC
    CAAAAGACCT TCACAGTATC TCAGGCAGGA AACTGCCGGA TCATGGCATA CTGTGATGCT
    CTGAGCTGCC TGGTGATATC ACAGCCTTCT CCTCAGGCCT CTTTTCTTCC AGGCTTTGGT
    GTTAAGATGT TGAGTACTGC CAACATGAAG AGCAGTCAGT ACATTCCGAT GCATGGCAAA
    CAGATCCGTG GACTGGCGTT TAGCAGTTAC CTCAGAGGCT TGCTACTCTC TGCTTCCCTA
    GACAACACTA TTAAACTGAC CAGCCTGGAG ACAAATACCG TGGTCCAGAC TTATAATGCT
    GGACGTCCTG TCTGGAGCTG TTGCTGGTGT CTTGATGAGG CTAACTACAT CTATGCTGGA
    CTGGCCAATG GTTCAATTCT GGTATATGAC GTGCGAAACA CGAGCAGTCA TGTGCAGGAG
    TTAGTAGCTC AGAAAGCCAG ATGCCCACTG GTCTCCCTGT CATACATGCC CAGAGCTGCC
    TCAGCTGCAT TTCCATATGG TGGGGTGCTG GCTGGAACCT TGGAGGATGC TTCATTCTGG
    GAACAGAAAA TGGACTTTTC TCATTGGCCT CATGTGCTGC CCTTGGAGCC AGGGGGCTGC
    ATAGACTTTC AGACAGAGAA CAGCTCCCGG CACTGTCTTG TGACCTACAG GCCTGATAAA
    AATCACACCA CCATACGAAG TGTGCTGATG GAAATGTCCT ACCGACTGGA TGACACTGGA
    AATCCAATCT GCTCCTGCCA GCCTGTACAT ACATTTTTTG GAGGACCTAC TTGCAAACTA
    TTGACCAAAA ATGCCATTTT CCAAAGCCCA GAGAATGATG GCAACATCCT GGTGTGTACT
    GGGGATGAAG CAGCAAATTC TGCCCTGCTG TGGGATGCTG CCAGTGGCTC GTTGCTCCAG
    GACCTACAGA CCGATCAGCC TGTGTTGGAC ATCTGCCCAT TTGAGGTGAA CCGTAACAGC
    TACTTGGCTA CCTTAACAGA GAAGATGGTC CACATCTATA AGTGGGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu108103
    Clone ID Related Accession (Same CDS sequence) NM_001370537.1 , NM_001370539.1 , NM_001370540.1 , NM_001370543.1 , NM_001370542.1
    Accession Version NM_001370542.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1491bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase RFWD3 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC109599.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.152782.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGATGAGG AAGAAGGGGA CACTTGTACA ATATGTCTGG AACAGTGGAC CAATGCTGGG 
    GACCACCGGC TCTCAGCATT ACGCTGTGGG CATCTCTTTG GGTATAGGTG CATTTCCACG
    TGGCTTAAAG GACAAGTACG AAAATGTCCC CAGTGCAACA AGAAAGCCAG GCACAGTGAC
    ATTGTCGTCC TTTATGCCCG AACCCTGAGA GCTTTGGACA CTAGTGAACA GGAGCGCATG
    AAAAGTTCCC TACTGAAGGA ACAGATGCTA AGGAAACAGG CCGAGTTAGA ATCAGCACAG
    TGCCGACTCC AACTGCAGGT CCTCACTGAT AAGTGCACTA GGCTTCAAAG GCGTGTTCAG
    GACTTGCAAA AACTTACGTC ACATCAAAGT CAGAATTTAC AGCAACCCAG GGGCTCCCAA
    GCATGGGTCC TGAGCTGCTC ACCCTCCAGC CAGGGCCAGC ACAAGCACAA GTACCACTTC
    CAAAAGACCT TCACAGTATC TCAGGCAGGA AACTGCCGGA TCATGGCATA CTGTGATGCT
    CTGAGCTGCC TGGTGATATC ACAGCCTTCT CCTCAGGCCT CTTTTCTTCC AGGCTTTGGT
    GTTAAGATGT TGAGTACTGC CAACATGAAG AGCAGTCAGT ACATTCCGAT GCATGGCAAA
    CAGATCCGTG GACTGGCGTT TAGCAGTTAC CTCAGAGGCT TGCTACTCTC TGCTTCCCTA
    GACAACACTA TTAAACTGAC CAGCCTGGAG ACAAATACCG TGGTCCAGAC TTATAATGCT
    GGACGTCCTG TCTGGAGCTG TTGCTGGTGT CTTGATGAGG CTAACTACAT CTATGCTGGA
    CTGGCCAATG GTTCAATTCT GGTATATGAC GTGCGAAACA CGAGCAGTCA TGTGCAGGAG
    TTAGTAGCTC AGAAAGCCAG ATGCCCACTG GTCTCCCTGT CATACATGCC CAGAGCTGCC
    TCAGCTGCAT TTCCATATGG TGGGGTGCTG GCTGGAACCT TGGAGGATGC TTCATTCTGG
    GAACAGAAAA TGGACTTTTC TCATTGGCCT CATGTGCTGC CCTTGGAGCC AGGGGGCTGC
    ATAGACTTTC AGACAGAGAA CAGCTCCCGG CACTGTCTTG TGACCTACAG GCCTGATAAA
    AATCACACCA CCATACGAAG TGTGCTGATG GAAATGTCCT ACCGACTGGA TGACACTGGA
    AATCCAATCT GCTCCTGCCA GCCTGTACAT ACATTTTTTG GAGGACCTAC TTGCAAACTA
    TTGACCAAAA ATGCCATTTT CCAAAGCCCA GAGAATGATG GCAACATCCT GGTGTGTACT
    GGGGATGAAG CAGCAAATTC TGCCCTGCTG TGGGATGCTG CCAGTGGCTC GTTGCTCCAG
    GACCTACAGA CCGATCAGCC TGTGTTGGAC ATCTGCCCAT TTGAGGTGAA CCGTAACAGC
    TACTTGGCTA CCTTAACAGA GAAGATGGTC CACATCTATA AGTGGGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001357471.1
    CDS1102..2592
    Translation

    Target ORF information:

    RefSeq Version NM_001370542.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001370542.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGATGAGG AAGAAGGGGA CACTTGTACA ATATGTCTGG AACAGTGGAC CAATGCTGGG 
    GACCACCGGC TCTCAGCATT ACGCTGTGGG CATCTCTTTG GGTATAGGTG CATTTCCACG
    TGGCTTAAAG GACAAGTACG AAAATGTCCC CAGTGCAACA AGAAAGCCAG GCACAGTGAC
    ATTGTCGTCC TTTATGCCCG AACCCTGAGA GCTTTGGACA CTAGTGAACA GGAGCGCATG
    AAAAGTTCCC TACTGAAGGA ACAGATGCTA AGGAAACAGG CCGAGTTAGA ATCAGCACAG
    TGCCGACTCC AACTGCAGGT CCTCACTGAT AAGTGCACTA GGCTTCAAAG GCGTGTTCAG
    GACTTGCAAA AACTTACGTC ACATCAAAGT CAGAATTTAC AGCAACCCAG GGGCTCCCAA
    GCATGGGTCC TGAGCTGCTC ACCCTCCAGC CAGGGCCAGC ACAAGCACAA GTACCACTTC
    CAAAAGACCT TCACAGTATC TCAGGCAGGA AACTGCCGGA TCATGGCATA CTGTGATGCT
    CTGAGCTGCC TGGTGATATC ACAGCCTTCT CCTCAGGCCT CTTTTCTTCC AGGCTTTGGT
    GTTAAGATGT TGAGTACTGC CAACATGAAG AGCAGTCAGT ACATTCCGAT GCATGGCAAA
    CAGATCCGTG GACTGGCGTT TAGCAGTTAC CTCAGAGGCT TGCTACTCTC TGCTTCCCTA
    GACAACACTA TTAAACTGAC CAGCCTGGAG ACAAATACCG TGGTCCAGAC TTATAATGCT
    GGACGTCCTG TCTGGAGCTG TTGCTGGTGT CTTGATGAGG CTAACTACAT CTATGCTGGA
    CTGGCCAATG GTTCAATTCT GGTATATGAC GTGCGAAACA CGAGCAGTCA TGTGCAGGAG
    TTAGTAGCTC AGAAAGCCAG ATGCCCACTG GTCTCCCTGT CATACATGCC CAGAGCTGCC
    TCAGCTGCAT TTCCATATGG TGGGGTGCTG GCTGGAACCT TGGAGGATGC TTCATTCTGG
    GAACAGAAAA TGGACTTTTC TCATTGGCCT CATGTGCTGC CCTTGGAGCC AGGGGGCTGC
    ATAGACTTTC AGACAGAGAA CAGCTCCCGG CACTGTCTTG TGACCTACAG GCCTGATAAA
    AATCACACCA CCATACGAAG TGTGCTGATG GAAATGTCCT ACCGACTGGA TGACACTGGA
    AATCCAATCT GCTCCTGCCA GCCTGTACAT ACATTTTTTG GAGGACCTAC TTGCAAACTA
    TTGACCAAAA ATGCCATTTT CCAAAGCCCA GAGAATGATG GCAACATCCT GGTGTGTACT
    GGGGATGAAG CAGCAAATTC TGCCCTGCTG TGGGATGCTG CCAGTGGCTC GTTGCTCCAG
    GACCTACAGA CCGATCAGCC TGTGTTGGAC ATCTGCCCAT TTGAGGTGAA CCGTAACAGC
    TACTTGGCTA CCTTAACAGA GAAGATGGTC CACATCTATA AGTGGGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02691
    Clone ID Related Accession (Same CDS sequence) NM_018124.3 , NM_001370534.1 , XM_017023391.1 , NM_018124.4 , XM_011523191.2 , XM_011523191.3 , XM_005256021.4 , NM_001370535.1 , XM_005256022.4
    Accession Version NM_018124.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2325bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-26
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase RFWD3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BP872110.1, BC059371.1, BC002574.2 and BM467919.1. On Jul 26, 2005 this sequence version replaced NM_018124.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.21672.1, SRR1660805.195981.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
    CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT
    GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG
    ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG
    ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA
    CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG
    ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA
    TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG
    GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT
    TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT
    AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC
    TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA
    GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC
    CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT
    GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC
    CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT
    AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC
    GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT
    TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA
    CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG
    CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG
    GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG
    ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC
    TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG
    ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC
    CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC
    ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT
    CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC
    AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA
    GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT
    GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG
    AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC
    TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC
    ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA
    ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC
    AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT
    GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA
    CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG
    GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_060594.3
    CDS99..2423
    Translation

    Target ORF information:

    RefSeq Version NM_018124.3
    Organism Homo sapiens(human)
    Definition Homo sapiens ring finger and WD repeat domain 3 (RFWD3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_018124.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
    CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT
    GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG
    ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG
    ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA
    CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG
    ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA
    TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG
    GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT
    TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT
    AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC
    TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA
    GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC
    CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT
    GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC
    CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT
    AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC
    GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT
    TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA
    CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG
    CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG
    GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG
    ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC
    TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG
    ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC
    CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC
    ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT
    CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC
    AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA
    GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT
    GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG
    AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC
    TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC
    ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA
    ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC
    AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT
    GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA
    CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG
    GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02691
    Clone ID Related Accession (Same CDS sequence) NM_018124.3 , NM_001370534.1 , XM_017023391.1 , NM_018124.4 , XM_011523191.2 , XM_011523191.3 , XM_005256021.4 , NM_001370535.1 , XM_005256022.4
    Accession Version NM_001370534.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2325bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-06
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase RFWD3 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC109599.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.255154.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
    CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT
    GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG
    ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG
    ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA
    CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG
    ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA
    TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG
    GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT
    TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT
    AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC
    TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA
    GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC
    CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT
    GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC
    CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT
    AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC
    GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT
    TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA
    CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG
    CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG
    GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG
    ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC
    TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG
    ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC
    CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC
    ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT
    CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC
    AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA
    GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT
    GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG
    AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC
    TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC
    ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA
    ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC
    AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT
    GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA
    CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG
    GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001357463.1
    CDS39..2363
    Translation

    Target ORF information:

    RefSeq Version NM_001370534.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001370534.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
    CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT
    GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG
    ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG
    ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA
    CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG
    ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA
    TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG
    GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT
    TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT
    AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC
    TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA
    GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC
    CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT
    GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC
    CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT
    AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC
    GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT
    TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA
    CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG
    CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG
    GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG
    ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC
    TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG
    ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC
    CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC
    ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT
    CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC
    AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA
    GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT
    GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG
    AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC
    TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC
    ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA
    ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC
    AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT
    GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA
    CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG
    GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02691
    Clone ID Related Accession (Same CDS sequence) NM_018124.3 , NM_001370534.1 , XM_017023391.1 , NM_018124.4 , XM_011523191.2 , XM_011523191.3 , XM_005256021.4 , NM_001370535.1 , XM_005256022.4
    Accession Version NM_001370535.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2325bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase RFWD3 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC109599.4. On May 9, 2019 this sequence version replaced XM_005256021.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.110044.1, SRR1803611.149781.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
    CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT
    GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG
    ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG
    ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA
    CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG
    ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA
    TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG
    GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT
    TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT
    AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC
    TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA
    GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC
    CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT
    GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC
    CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT
    AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC
    GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT
    TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA
    CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG
    CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG
    GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG
    ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC
    TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG
    ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC
    CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC
    ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT
    CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC
    AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA
    GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT
    GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG
    AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC
    TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC
    ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA
    ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC
    AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT
    GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA
    CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG
    GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001357464.1
    CDS217..2541
    Translation

    Target ORF information:

    RefSeq Version NM_001370535.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001370535.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
    CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT
    GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG
    ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG
    ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA
    CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG
    ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA
    TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG
    GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT
    TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT
    AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC
    TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA
    GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC
    CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT
    GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC
    CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT
    AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC
    GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT
    TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA
    CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG
    CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG
    GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG
    ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC
    TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG
    ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC
    CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC
    ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT
    CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC
    AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA
    GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT
    GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG
    AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC
    TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC
    ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA
    ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC
    AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT
    GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA
    CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG
    GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu49413
    Clone ID Related Accession (Same CDS sequence) NM_001370536.1 , XM_006721228.3 , XM_017023392.1
    Accession Version NM_001370536.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2148bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase RFWD3 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC109599.4. On May 9, 2019 this sequence version replaced XM_017023392.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.31049.1, SRR1803613.79167.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
    CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT
    GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG
    ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG
    ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA
    CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG
    ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA
    TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG
    GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT
    TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT
    AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC
    TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA
    GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC
    CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT
    GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC
    CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT
    AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC
    GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT
    TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA
    CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG
    CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG
    GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG
    ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC
    TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG
    ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC
    CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC
    ACTATTAAAC TGACCAGATG CCCACTGGTC TCCCTGTCAT ACATGCCCAG AGCTGCCTCA
    GCTGCATTTC CATATGGTGG GGTGCTGGCT GGAACCTTGG AGGATGCTTC ATTCTGGGAA
    CAGAAAATGG ACTTTTCTCA TTGGCCTCAT GTGCTGCCCT TGGAGCCAGG GGGCTGCATA
    GACTTTCAGA CAGAGAACAG CTCCCGGCAC TGTCTTGTGA CCTACAGGCC TGATAAAAAT
    CACACCACCA TACGAAGTGT GCTGATGGAA ATGTCCTACC GACTGGATGA CACTGGAAAT
    CCAATCTGCT CCTGCCAGCC TGTACATACA TTTTTTGGAG GACCTACTTG CAAACTATTG
    ACCAAAAATG CCATTTTCCA AAGCCCAGAG AATGATGGCA ACATCCTGGT GTGTACTGGG
    GATGAAGCAG CAAATTCTGC CCTGCTGTGG GATGCTGCCA GTGGCTCGTT GCTCCAGGAC
    CTACAGACCG ATCAGCCTGT GTTGGACATC TGCCCATTTG AGGTGAACCG TAACAGCTAC
    TTGGCTACCT TAACAGAGAA GATGGTCCAC ATCTATAAGT GGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001357465.1
    CDS95..2242
    Translation

    Target ORF information:

    RefSeq Version NM_001370536.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001370536.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
    CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT
    GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG
    ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG
    ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA
    CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG
    ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA
    TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG
    GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT
    TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT
    AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC
    TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA
    GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC
    CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT
    GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC
    CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT
    AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC
    GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT
    TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA
    CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG
    CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG
    GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG
    ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC
    TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG
    ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC
    CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC
    ACTATTAAAC TGACCAGATG CCCACTGGTC TCCCTGTCAT ACATGCCCAG AGCTGCCTCA
    GCTGCATTTC CATATGGTGG GGTGCTGGCT GGAACCTTGG AGGATGCTTC ATTCTGGGAA
    CAGAAAATGG ACTTTTCTCA TTGGCCTCAT GTGCTGCCCT TGGAGCCAGG GGGCTGCATA
    GACTTTCAGA CAGAGAACAG CTCCCGGCAC TGTCTTGTGA CCTACAGGCC TGATAAAAAT
    CACACCACCA TACGAAGTGT GCTGATGGAA ATGTCCTACC GACTGGATGA CACTGGAAAT
    CCAATCTGCT CCTGCCAGCC TGTACATACA TTTTTTGGAG GACCTACTTG CAAACTATTG
    ACCAAAAATG CCATTTTCCA AAGCCCAGAG AATGATGGCA ACATCCTGGT GTGTACTGGG
    GATGAAGCAG CAAATTCTGC CCTGCTGTGG GATGCTGCCA GTGGCTCGTT GCTCCAGGAC
    CTACAGACCG ATCAGCCTGT GTTGGACATC TGCCCATTTG AGGTGAACCG TAACAGCTAC
    TTGGCTACCT TAACAGAGAA GATGGTCCAC ATCTATAAGT GGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02691
    Clone ID Related Accession (Same CDS sequence) NM_018124.3 , NM_001370534.1 , XM_017023391.1 , NM_018124.4 , XM_011523191.2 , XM_011523191.3 , XM_005256021.4 , NM_001370535.1 , XM_005256022.4
    Accession Version XM_017023391.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2325bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase RFWD3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
    CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT
    GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG
    ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG
    ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA
    CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG
    ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA
    TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG
    GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT
    TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT
    AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC
    TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA
    GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC
    CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT
    GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC
    CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT
    AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC
    GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT
    TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA
    CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG
    CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG
    GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG
    ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC
    TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG
    ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC
    CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC
    ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT
    CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC
    AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA
    GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT
    GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG
    AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC
    TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC
    ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA
    ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC
    AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT
    GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA
    CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG
    GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016878880.1
    CDS148..2472
    Translation

    Target ORF information:

    RefSeq Version XM_017023391.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017023391.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
    CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT
    GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG
    ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG
    ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA
    CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG
    ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA
    TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG
    GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT
    TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT
    AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC
    TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA
    GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC
    CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT
    GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC
    CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT
    AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC
    GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT
    TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA
    CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG
    CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG
    GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG
    ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC
    TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG
    ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC
    CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC
    ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT
    CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC
    AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA
    GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT
    GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG
    AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC
    TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC
    ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA
    ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC
    AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT
    GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA
    CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG
    GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02691
    Clone ID Related Accession (Same CDS sequence) NM_018124.3 , NM_001370534.1 , XM_017023391.1 , NM_018124.4 , XM_011523191.2 , XM_011523191.3 , XM_005256021.4 , NM_001370535.1 , XM_005256022.4
    Accession Version XM_011523191.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2325bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase RFWD3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011523191.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
    CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT
    GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG
    ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG
    ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA
    CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG
    ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA
    TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG
    GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT
    TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT
    AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC
    TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA
    GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC
    CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT
    GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC
    CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT
    AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC
    GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT
    TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA
    CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG
    CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG
    GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG
    ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC
    TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG
    ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC
    CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC
    ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT
    CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC
    AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA
    GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT
    GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG
    AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC
    TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC
    ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA
    ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC
    AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT
    GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA
    CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG
    GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011521493.1
    CDS52..2376
    Translation

    Target ORF information:

    RefSeq Version XM_011523191.2
    Organism Homo sapiens(human)
    Definition Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011523191.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
    CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT
    GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG
    ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG
    ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA
    CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG
    ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA
    TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG
    GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT
    TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT
    AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC
    TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA
    GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC
    CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT
    GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC
    CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT
    AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC
    GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT
    TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA
    CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG
    CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG
    GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG
    ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC
    TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG
    ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC
    CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC
    ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT
    CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC
    AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA
    GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT
    GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG
    AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC
    TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC
    ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA
    ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC
    AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT
    GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA
    CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG
    GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02691
    Clone ID Related Accession (Same CDS sequence) NM_018124.3 , NM_001370534.1 , XM_017023391.1 , NM_018124.4 , XM_011523191.2 , XM_011523191.3 , XM_005256021.4 , NM_001370535.1 , XM_005256022.4
    Accession Version XM_011523191.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2325bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase RFWD3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011523191.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
    CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT
    GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG
    ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG
    ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA
    CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG
    ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA
    TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG
    GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT
    TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT
    AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC
    TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA
    GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC
    CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT
    GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC
    CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT
    AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC
    GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT
    TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA
    CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG
    CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG
    GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG
    ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC
    TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG
    ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC
    CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC
    ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT
    CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC
    AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA
    GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT
    GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG
    AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC
    TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC
    ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA
    ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC
    AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT
    GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA
    CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG
    GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011521493.1
    CDS64..2388
    Translation

    Target ORF information:

    RefSeq Version XM_011523191.3
    Organism Homo sapiens(human)
    Definition Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011523191.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
    CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT
    GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG
    ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG
    ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA
    CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG
    ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA
    TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG
    GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT
    TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT
    AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC
    TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA
    GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC
    CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT
    GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC
    CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT
    AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC
    GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT
    TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA
    CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG
    CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG
    GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG
    ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC
    TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG
    ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC
    CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC
    ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT
    CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC
    AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA
    GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT
    GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG
    AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC
    TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC
    ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA
    ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC
    AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT
    GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA
    CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG
    GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02691
    Clone ID Related Accession (Same CDS sequence) NM_018124.3 , NM_001370534.1 , XM_017023391.1 , NM_018124.4 , XM_011523191.2 , XM_011523191.3 , XM_005256021.4 , NM_001370535.1 , XM_005256022.4
    Accession Version XM_005256021.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2325bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase RFWD3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005256021.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
    CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT
    GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG
    ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG
    ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA
    CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG
    ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA
    TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG
    GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT
    TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT
    AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC
    TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA
    GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC
    CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT
    GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC
    CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT
    AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC
    GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT
    TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA
    CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG
    CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG
    GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG
    ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC
    TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG
    ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC
    CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC
    ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT
    CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC
    AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA
    GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT
    GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG
    AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC
    TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC
    ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA
    ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC
    AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT
    GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA
    CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG
    GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005256078.1
    CDS232..2556
    Translation

    Target ORF information:

    RefSeq Version XM_005256021.4
    Organism Homo sapiens(human)
    Definition Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005256021.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
    CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT
    GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG
    ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG
    ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA
    CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG
    ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA
    TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG
    GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT
    TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT
    AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC
    TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA
    GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC
    CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT
    GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC
    CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT
    AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC
    GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT
    TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA
    CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG
    CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG
    GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG
    ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC
    TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG
    ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC
    CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC
    ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT
    CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC
    AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA
    GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT
    GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG
    AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC
    TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC
    ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA
    ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC
    AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT
    GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA
    CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG
    GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02691
    Clone ID Related Accession (Same CDS sequence) NM_018124.3 , NM_001370534.1 , XM_017023391.1 , NM_018124.4 , XM_011523191.2 , XM_011523191.3 , XM_005256021.4 , NM_001370535.1 , XM_005256022.4
    Accession Version XM_005256022.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2325bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase RFWD3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005256022.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
    CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT
    GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG
    ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG
    ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA
    CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG
    ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA
    TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG
    GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT
    TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT
    AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC
    TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA
    GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC
    CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT
    GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC
    CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT
    AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC
    GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT
    TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA
    CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG
    CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG
    GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG
    ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC
    TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG
    ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC
    CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC
    ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT
    CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC
    AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA
    GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT
    GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG
    AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC
    TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC
    ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA
    ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC
    AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT
    GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA
    CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG
    GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005256079.1
    CDS982..3306
    Translation

    Target ORF information:

    RefSeq Version XM_005256022.4
    Organism Homo sapiens(human)
    Definition Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005256022.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
    CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT
    GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG
    ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG
    ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA
    CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG
    ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA
    TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG
    GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT
    TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT
    AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC
    TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA
    GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC
    CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT
    GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC
    CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT
    AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC
    GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT
    TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA
    CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG
    CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG
    GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG
    ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC
    TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG
    ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC
    CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC
    ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT
    CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC
    AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA
    GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT
    GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG
    AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC
    TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC
    ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA
    ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC
    AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT
    GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA
    CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG
    GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu49413
    Clone ID Related Accession (Same CDS sequence) NM_001370536.1 , XM_006721228.3 , XM_017023392.1
    Accession Version XM_006721228.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2148bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase RFWD3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006721228.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
    CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT
    GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG
    ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG
    ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA
    CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG
    ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA
    TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG
    GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT
    TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT
    AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC
    TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA
    GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC
    CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT
    GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC
    CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT
    AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC
    GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT
    TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA
    CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG
    CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG
    GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG
    ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC
    TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG
    ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC
    CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC
    ACTATTAAAC TGACCAGATG CCCACTGGTC TCCCTGTCAT ACATGCCCAG AGCTGCCTCA
    GCTGCATTTC CATATGGTGG GGTGCTGGCT GGAACCTTGG AGGATGCTTC ATTCTGGGAA
    CAGAAAATGG ACTTTTCTCA TTGGCCTCAT GTGCTGCCCT TGGAGCCAGG GGGCTGCATA
    GACTTTCAGA CAGAGAACAG CTCCCGGCAC TGTCTTGTGA CCTACAGGCC TGATAAAAAT
    CACACCACCA TACGAAGTGT GCTGATGGAA ATGTCCTACC GACTGGATGA CACTGGAAAT
    CCAATCTGCT CCTGCCAGCC TGTACATACA TTTTTTGGAG GACCTACTTG CAAACTATTG
    ACCAAAAATG CCATTTTCCA AAGCCCAGAG AATGATGGCA ACATCCTGGT GTGTACTGGG
    GATGAAGCAG CAAATTCTGC CCTGCTGTGG GATGCTGCCA GTGGCTCGTT GCTCCAGGAC
    CTACAGACCG ATCAGCCTGT GTTGGACATC TGCCCATTTG AGGTGAACCG TAACAGCTAC
    TTGGCTACCT TAACAGAGAA GATGGTCCAC ATCTATAAGT GGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006721291.1
    CDS232..2379
    Translation

    Target ORF information:

    RefSeq Version XM_006721228.3
    Organism Homo sapiens(human)
    Definition Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006721228.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
    CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT
    GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG
    ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG
    ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA
    CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG
    ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA
    TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG
    GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT
    TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT
    AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC
    TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA
    GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC
    CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT
    GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC
    CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT
    AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC
    GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT
    TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA
    CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG
    CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG
    GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG
    ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC
    TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG
    ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC
    CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC
    ACTATTAAAC TGACCAGATG CCCACTGGTC TCCCTGTCAT ACATGCCCAG AGCTGCCTCA
    GCTGCATTTC CATATGGTGG GGTGCTGGCT GGAACCTTGG AGGATGCTTC ATTCTGGGAA
    CAGAAAATGG ACTTTTCTCA TTGGCCTCAT GTGCTGCCCT TGGAGCCAGG GGGCTGCATA
    GACTTTCAGA CAGAGAACAG CTCCCGGCAC TGTCTTGTGA CCTACAGGCC TGATAAAAAT
    CACACCACCA TACGAAGTGT GCTGATGGAA ATGTCCTACC GACTGGATGA CACTGGAAAT
    CCAATCTGCT CCTGCCAGCC TGTACATACA TTTTTTGGAG GACCTACTTG CAAACTATTG
    ACCAAAAATG CCATTTTCCA AAGCCCAGAG AATGATGGCA ACATCCTGGT GTGTACTGGG
    GATGAAGCAG CAAATTCTGC CCTGCTGTGG GATGCTGCCA GTGGCTCGTT GCTCCAGGAC
    CTACAGACCG ATCAGCCTGT GTTGGACATC TGCCCATTTG AGGTGAACCG TAACAGCTAC
    TTGGCTACCT TAACAGAGAA GATGGTCCAC ATCTATAAGT GGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu49413
    Clone ID Related Accession (Same CDS sequence) NM_001370536.1 , XM_006721228.3 , XM_017023392.1
    Accession Version XM_017023392.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2148bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase RFWD3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
    CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT
    GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG
    ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG
    ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA
    CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG
    ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA
    TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG
    GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT
    TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT
    AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC
    TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA
    GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC
    CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT
    GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC
    CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT
    AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC
    GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT
    TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA
    CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG
    CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG
    GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG
    ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC
    TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG
    ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC
    CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC
    ACTATTAAAC TGACCAGATG CCCACTGGTC TCCCTGTCAT ACATGCCCAG AGCTGCCTCA
    GCTGCATTTC CATATGGTGG GGTGCTGGCT GGAACCTTGG AGGATGCTTC ATTCTGGGAA
    CAGAAAATGG ACTTTTCTCA TTGGCCTCAT GTGCTGCCCT TGGAGCCAGG GGGCTGCATA
    GACTTTCAGA CAGAGAACAG CTCCCGGCAC TGTCTTGTGA CCTACAGGCC TGATAAAAAT
    CACACCACCA TACGAAGTGT GCTGATGGAA ATGTCCTACC GACTGGATGA CACTGGAAAT
    CCAATCTGCT CCTGCCAGCC TGTACATACA TTTTTTGGAG GACCTACTTG CAAACTATTG
    ACCAAAAATG CCATTTTCCA AAGCCCAGAG AATGATGGCA ACATCCTGGT GTGTACTGGG
    GATGAAGCAG CAAATTCTGC CCTGCTGTGG GATGCTGCCA GTGGCTCGTT GCTCCAGGAC
    CTACAGACCG ATCAGCCTGT GTTGGACATC TGCCCATTTG AGGTGAACCG TAACAGCTAC
    TTGGCTACCT TAACAGAGAA GATGGTCCAC ATCTATAAGT GGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016878881.1
    CDS96..2243
    Translation

    Target ORF information:

    RefSeq Version XM_017023392.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017023392.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
    CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT
    GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG
    ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG
    ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA
    CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG
    ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA
    TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG
    GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT
    TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT
    AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC
    TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA
    GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC
    CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT
    GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC
    CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT
    AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC
    GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT
    TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA
    CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG
    CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG
    GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG
    ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC
    TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG
    ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC
    CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC
    ACTATTAAAC TGACCAGATG CCCACTGGTC TCCCTGTCAT ACATGCCCAG AGCTGCCTCA
    GCTGCATTTC CATATGGTGG GGTGCTGGCT GGAACCTTGG AGGATGCTTC ATTCTGGGAA
    CAGAAAATGG ACTTTTCTCA TTGGCCTCAT GTGCTGCCCT TGGAGCCAGG GGGCTGCATA
    GACTTTCAGA CAGAGAACAG CTCCCGGCAC TGTCTTGTGA CCTACAGGCC TGATAAAAAT
    CACACCACCA TACGAAGTGT GCTGATGGAA ATGTCCTACC GACTGGATGA CACTGGAAAT
    CCAATCTGCT CCTGCCAGCC TGTACATACA TTTTTTGGAG GACCTACTTG CAAACTATTG
    ACCAAAAATG CCATTTTCCA AAGCCCAGAG AATGATGGCA ACATCCTGGT GTGTACTGGG
    GATGAAGCAG CAAATTCTGC CCTGCTGTGG GATGCTGCCA GTGGCTCGTT GCTCCAGGAC
    CTACAGACCG ATCAGCCTGT GTTGGACATC TGCCCATTTG AGGTGAACCG TAACAGCTAC
    TTGGCTACCT TAACAGAGAA GATGGTCCAC ATCTATAAGT GGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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