PPT1 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following PPT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu26235 NM_000310.4
Latest version!
Homo sapiens palmitoyl-protein thioesterase 1 (PPT1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$154.50-$216.30
$309.00
OHu26114 NM_001142604.2
Latest version!
Homo sapiens palmitoyl-protein thioesterase 1 (PPT1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $188.30
$269.00
OHu33593 NM_001363695.2
Latest version!
Homo sapiens palmitoyl-protein thioesterase 1 (PPT1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OHu33593 XM_005271008.1
Latest version!
Homo sapiens palmitoyl-protein thioesterase 1 (PPT1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
View Old Accession Versions >>
OHu26235 NM_000310.3 Homo sapiens palmitoyl-protein thioesterase 1 (PPT1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$154.50-$216.30
$309.00
OHu26114 NM_001142604.1 Homo sapiens palmitoyl-protein thioesterase 1 (PPT1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $188.30
$269.00
OHu33593 NM_001363695.1 Homo sapiens palmitoyl-protein thioesterase 1 (PPT1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu26235
    Clone ID Related Accession (Same CDS sequence) NM_000310.4 , NM_000310.3
    Accession Version NM_000310.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 921bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-20
    Organism Homo sapiens(human)
    Product palmitoyl-protein thioesterase 1 isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA504318.1, BC008426.1 and AL512599.33. On Nov 21, 2019 this sequence version replaced NM_000310.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC008426.1, U44772.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGCGTCGC CCGGCTGCCT GTGGCTCTTG GCTGTGGCTC TCCTGCCATG GACCTGCGCT 
    TCTCGGGCGC TGCAGCATCT GGACCCGCCG GCGCCGCTGC CGTTGGTGAT CTGGCATGGG
    ATGGGAGACA GCTGTTGCAA TCCCTTAAGC ATGGGTGCTA TTAAAAAAAT GGTGGAGAAG
    AAAATACCTG GAATTTACGT CTTATCTTTA GAGATTGGGA AGACCCTGAT GGAGGACGTG
    GAGAACAGCT TCTTCTTGAA TGTCAATTCC CAAGTAACAA CAGTGTGTCA GGCACTTGCT
    AAGGATCCTA AATTGCAGCA AGGCTACAAT GCTATGGGAT TCTCCCAGGG AGGCCAATTT
    CTGAGGGCAG TGGCTCAGAG ATGCCCTTCA CCTCCCATGA TCAATCTGAT CTCGGTTGGG
    GGACAACATC AAGGTGTTTT TGGACTCCCT CGATGCCCAG GAGAGAGCTC TCACATCTGT
    GACTTCATCC GAAAAACACT GAATGCTGGG GCGTACTCCA AAGTTGTTCA GGAACGCCTC
    GTGCAAGCCG AATACTGGCA TGACCCCATA AAGGAGGATG TGTATCGCAA CCACAGCATC
    TTCTTGGCAG ATATAAATCA GGAGCGGGGT ATCAATGAGT CCTACAAGAA AAACCTGATG
    GCCCTGAAGA AGTTTGTGAT GGTGAAATTC CTCAATGATT CCATTGTGGA CCCTGTAGAT
    TCGGAGTGGT TTGGATTTTA CAGAAGTGGC CAAGCCAAGG AAACCATTCC CTTACAGGAG
    ACCTCCCTGT ACACACAGGA CCGCCTGGGG CTAAAGGAAA TGGACAATGC AGGACAGCTA
    GTGTTTCTGG CTACAGAAGG GGACCATCTT CAGTTGTCTG AAGAATGGTT TTATGCCCAC
    ATCATACCAT TCCTTGGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000301.1
    CDS15..935
    Translation

    Target ORF information:

    RefSeq Version NM_000310.4
    Organism Homo sapiens(human)
    Definition Homo sapiens palmitoyl-protein thioesterase 1 (PPT1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000310.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGCGTCGC CCGGCTGCCT GTGGCTCTTG GCTGTGGCTC TCCTGCCATG GACCTGCGCT 
    TCTCGGGCGC TGCAGCATCT GGACCCGCCG GCGCCGCTGC CGTTGGTGAT CTGGCATGGG
    ATGGGAGACA GCTGTTGCAA TCCCTTAAGC ATGGGTGCTA TTAAAAAAAT GGTGGAGAAG
    AAAATACCTG GAATTTACGT CTTATCTTTA GAGATTGGGA AGACCCTGAT GGAGGACGTG
    GAGAACAGCT TCTTCTTGAA TGTCAATTCC CAAGTAACAA CAGTGTGTCA GGCACTTGCT
    AAGGATCCTA AATTGCAGCA AGGCTACAAT GCTATGGGAT TCTCCCAGGG AGGCCAATTT
    CTGAGGGCAG TGGCTCAGAG ATGCCCTTCA CCTCCCATGA TCAATCTGAT CTCGGTTGGG
    GGACAACATC AAGGTGTTTT TGGACTCCCT CGATGCCCAG GAGAGAGCTC TCACATCTGT
    GACTTCATCC GAAAAACACT GAATGCTGGG GCGTACTCCA AAGTTGTTCA GGAACGCCTC
    GTGCAAGCCG AATACTGGCA TGACCCCATA AAGGAGGATG TGTATCGCAA CCACAGCATC
    TTCTTGGCAG ATATAAATCA GGAGCGGGGT ATCAATGAGT CCTACAAGAA AAACCTGATG
    GCCCTGAAGA AGTTTGTGAT GGTGAAATTC CTCAATGATT CCATTGTGGA CCCTGTAGAT
    TCGGAGTGGT TTGGATTTTA CAGAAGTGGC CAAGCCAAGG AAACCATTCC CTTACAGGAG
    ACCTCCCTGT ACACACAGGA CCGCCTGGGG CTAAAGGAAA TGGACAATGC AGGACAGCTA
    GTGTTTCTGG CTACAGAAGG GGACCATCTT CAGTTGTCTG AAGAATGGTT TTATGCCCAC
    ATCATACCAT TCCTTGGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26235
    Clone ID Related Accession (Same CDS sequence) NM_000310.4 , NM_000310.3
    Accession Version NM_000310.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 921bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-03
    Organism Homo sapiens(human)
    Product palmitoyl-protein thioesterase 1 isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA504318.1, AL512599.33 and BC008426.1. This sequence is a reference standard in the RefSeqGene project. On Dec 11, 2008 this sequence version replaced NM_000310.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.337638.1, SRR1803611.208044.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142670, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGCGTCGC CCGGCTGCCT GTGGCTCTTG GCTGTGGCTC TCCTGCCATG GACCTGCGCT 
    TCTCGGGCGC TGCAGCATCT GGACCCGCCG GCGCCGCTGC CGTTGGTGAT CTGGCATGGG
    ATGGGAGACA GCTGTTGCAA TCCCTTAAGC ATGGGTGCTA TTAAAAAAAT GGTGGAGAAG
    AAAATACCTG GAATTTACGT CTTATCTTTA GAGATTGGGA AGACCCTGAT GGAGGACGTG
    GAGAACAGCT TCTTCTTGAA TGTCAATTCC CAAGTAACAA CAGTGTGTCA GGCACTTGCT
    AAGGATCCTA AATTGCAGCA AGGCTACAAT GCTATGGGAT TCTCCCAGGG AGGCCAATTT
    CTGAGGGCAG TGGCTCAGAG ATGCCCTTCA CCTCCCATGA TCAATCTGAT CTCGGTTGGG
    GGACAACATC AAGGTGTTTT TGGACTCCCT CGATGCCCAG GAGAGAGCTC TCACATCTGT
    GACTTCATCC GAAAAACACT GAATGCTGGG GCGTACTCCA AAGTTGTTCA GGAACGCCTC
    GTGCAAGCCG AATACTGGCA TGACCCCATA AAGGAGGATG TGTATCGCAA CCACAGCATC
    TTCTTGGCAG ATATAAATCA GGAGCGGGGT ATCAATGAGT CCTACAAGAA AAACCTGATG
    GCCCTGAAGA AGTTTGTGAT GGTGAAATTC CTCAATGATT CCATTGTGGA CCCTGTAGAT
    TCGGAGTGGT TTGGATTTTA CAGAAGTGGC CAAGCCAAGG AAACCATTCC CTTACAGGAG
    ACCTCCCTGT ACACACAGGA CCGCCTGGGG CTAAAGGAAA TGGACAATGC AGGACAGCTA
    GTGTTTCTGG CTACAGAAGG GGACCATCTT CAGTTGTCTG AAGAATGGTT TTATGCCCAC
    ATCATACCAT TCCTTGGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000301.1
    CDS233..1153
    Translation

    Target ORF information:

    RefSeq Version NM_000310.3
    Organism Homo sapiens(human)
    Definition Homo sapiens palmitoyl-protein thioesterase 1 (PPT1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000310.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGCGTCGC CCGGCTGCCT GTGGCTCTTG GCTGTGGCTC TCCTGCCATG GACCTGCGCT 
    TCTCGGGCGC TGCAGCATCT GGACCCGCCG GCGCCGCTGC CGTTGGTGAT CTGGCATGGG
    ATGGGAGACA GCTGTTGCAA TCCCTTAAGC ATGGGTGCTA TTAAAAAAAT GGTGGAGAAG
    AAAATACCTG GAATTTACGT CTTATCTTTA GAGATTGGGA AGACCCTGAT GGAGGACGTG
    GAGAACAGCT TCTTCTTGAA TGTCAATTCC CAAGTAACAA CAGTGTGTCA GGCACTTGCT
    AAGGATCCTA AATTGCAGCA AGGCTACAAT GCTATGGGAT TCTCCCAGGG AGGCCAATTT
    CTGAGGGCAG TGGCTCAGAG ATGCCCTTCA CCTCCCATGA TCAATCTGAT CTCGGTTGGG
    GGACAACATC AAGGTGTTTT TGGACTCCCT CGATGCCCAG GAGAGAGCTC TCACATCTGT
    GACTTCATCC GAAAAACACT GAATGCTGGG GCGTACTCCA AAGTTGTTCA GGAACGCCTC
    GTGCAAGCCG AATACTGGCA TGACCCCATA AAGGAGGATG TGTATCGCAA CCACAGCATC
    TTCTTGGCAG ATATAAATCA GGAGCGGGGT ATCAATGAGT CCTACAAGAA AAACCTGATG
    GCCCTGAAGA AGTTTGTGAT GGTGAAATTC CTCAATGATT CCATTGTGGA CCCTGTAGAT
    TCGGAGTGGT TTGGATTTTA CAGAAGTGGC CAAGCCAAGG AAACCATTCC CTTACAGGAG
    ACCTCCCTGT ACACACAGGA CCGCCTGGGG CTAAAGGAAA TGGACAATGC AGGACAGCTA
    GTGTTTCTGG CTACAGAAGG GGACCATCTT CAGTTGTCTG AAGAATGGTT TTATGCCCAC
    ATCATACCAT TCCTTGGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26114
    Clone ID Related Accession (Same CDS sequence) NM_001142604.1 , NM_001142604.2
    Accession Version NM_001142604.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 612bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-19
    Organism Homo sapiens(human)
    Product palmitoyl-protein thioesterase 1 isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA504318.1, AL512599.33, AK302232.1 and BC008426.1. Transcript Variant: This variant (2) lacks an in-frame segment compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.3175.1, SRR1660805.321030.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820691 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGCGTCGC CCGGCTGCCT GTGGCTCTTG GCTGTGGCTC TCCTGCCATG GACCTGCGCT 
    TCTCGGGCGC TGCAGCATCT GGACCCGCCG GCGCCGCTGC CGTTGGTGAT CTGGCATGGG
    ATGGGTGTTT TTGGACTCCC TCGATGCCCA GGAGAGAGCT CTCACATCTG TGACTTCATC
    CGAAAAACAC TGAATGCTGG GGCGTACTCC AAAGTTGTTC AGGAACGCCT CGTGCAAGCC
    GAATACTGGC ATGACCCCAT AAAGGAGGAT GTGTATCGCA ACCACAGCAT CTTCTTGGCA
    GATATAAATC AGGAGCGGGG TATCAATGAG TCCTACAAGA AAAACCTGAT GGCCCTGAAG
    AAGTTTGTGA TGGTGAAATT CCTCAATGAT TCCATTGTGG ACCCTGTAGA TTCGGAGTGG
    TTTGGATTTT ACAGAAGTGG CCAAGCCAAG GAAACCATTC CCTTACAGGA GACCTCCCTG
    TACACACAGG ACCGCCTGGG GCTAAAGGAA ATGGACAATG CAGGACAGCT AGTGTTTCTG
    GCTACAGAAG GGGACCATCT TCAGTTGTCT GAAGAATGGT TTTATGCCCA CATCATACCA
    TTCCTTGGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001136076.1
    CDS233..844
    Translation

    Target ORF information:

    RefSeq Version NM_001142604.1
    Organism Homo sapiens(human)
    Definition Homo sapiens palmitoyl-protein thioesterase 1 (PPT1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001142604.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGCGTCGC CCGGCTGCCT GTGGCTCTTG GCTGTGGCTC TCCTGCCATG GACCTGCGCT 
    TCTCGGGCGC TGCAGCATCT GGACCCGCCG GCGCCGCTGC CGTTGGTGAT CTGGCATGGG
    ATGGGTGTTT TTGGACTCCC TCGATGCCCA GGAGAGAGCT CTCACATCTG TGACTTCATC
    CGAAAAACAC TGAATGCTGG GGCGTACTCC AAAGTTGTTC AGGAACGCCT CGTGCAAGCC
    GAATACTGGC ATGACCCCAT AAAGGAGGAT GTGTATCGCA ACCACAGCAT CTTCTTGGCA
    GATATAAATC AGGAGCGGGG TATCAATGAG TCCTACAAGA AAAACCTGAT GGCCCTGAAG
    AAGTTTGTGA TGGTGAAATT CCTCAATGAT TCCATTGTGG ACCCTGTAGA TTCGGAGTGG
    TTTGGATTTT ACAGAAGTGG CCAAGCCAAG GAAACCATTC CCTTACAGGA GACCTCCCTG
    TACACACAGG ACCGCCTGGG GCTAAAGGAA ATGGACAATG CAGGACAGCT AGTGTTTCTG
    GCTACAGAAG GGGACCATCT TCAGTTGTCT GAAGAATGGT TTTATGCCCA CATCATACCA
    TTCCTTGGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26114
    Clone ID Related Accession (Same CDS sequence) NM_001142604.1 , NM_001142604.2
    Accession Version NM_001142604.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 612bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-01
    Organism Homo sapiens(human)
    Product palmitoyl-protein thioesterase 1 isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK302232.1, BC008426.1 and AL512599.33. On Jun 2, 2019 this sequence version replaced NM_001142604.1. Transcript Variant: This variant (2) lacks an in-frame segment compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.3175.1, AK302232.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820691 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGCGTCGC CCGGCTGCCT GTGGCTCTTG GCTGTGGCTC TCCTGCCATG GACCTGCGCT 
    TCTCGGGCGC TGCAGCATCT GGACCCGCCG GCGCCGCTGC CGTTGGTGAT CTGGCATGGG
    ATGGGTGTTT TTGGACTCCC TCGATGCCCA GGAGAGAGCT CTCACATCTG TGACTTCATC
    CGAAAAACAC TGAATGCTGG GGCGTACTCC AAAGTTGTTC AGGAACGCCT CGTGCAAGCC
    GAATACTGGC ATGACCCCAT AAAGGAGGAT GTGTATCGCA ACCACAGCAT CTTCTTGGCA
    GATATAAATC AGGAGCGGGG TATCAATGAG TCCTACAAGA AAAACCTGAT GGCCCTGAAG
    AAGTTTGTGA TGGTGAAATT CCTCAATGAT TCCATTGTGG ACCCTGTAGA TTCGGAGTGG
    TTTGGATTTT ACAGAAGTGG CCAAGCCAAG GAAACCATTC CCTTACAGGA GACCTCCCTG
    TACACACAGG ACCGCCTGGG GCTAAAGGAA ATGGACAATG CAGGACAGCT AGTGTTTCTG
    GCTACAGAAG GGGACCATCT TCAGTTGTCT GAAGAATGGT TTTATGCCCA CATCATACCA
    TTCCTTGGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001136076.1
    CDS15..626
    Translation

    Target ORF information:

    RefSeq Version NM_001142604.2
    Organism Homo sapiens(human)
    Definition Homo sapiens palmitoyl-protein thioesterase 1 (PPT1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001142604.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGCGTCGC CCGGCTGCCT GTGGCTCTTG GCTGTGGCTC TCCTGCCATG GACCTGCGCT 
    TCTCGGGCGC TGCAGCATCT GGACCCGCCG GCGCCGCTGC CGTTGGTGAT CTGGCATGGG
    ATGGGTGTTT TTGGACTCCC TCGATGCCCA GGAGAGAGCT CTCACATCTG TGACTTCATC
    CGAAAAACAC TGAATGCTGG GGCGTACTCC AAAGTTGTTC AGGAACGCCT CGTGCAAGCC
    GAATACTGGC ATGACCCCAT AAAGGAGGAT GTGTATCGCA ACCACAGCAT CTTCTTGGCA
    GATATAAATC AGGAGCGGGG TATCAATGAG TCCTACAAGA AAAACCTGAT GGCCCTGAAG
    AAGTTTGTGA TGGTGAAATT CCTCAATGAT TCCATTGTGG ACCCTGTAGA TTCGGAGTGG
    TTTGGATTTT ACAGAAGTGG CCAAGCCAAG GAAACCATTC CCTTACAGGA GACCTCCCTG
    TACACACAGG ACCGCCTGGG GCTAAAGGAA ATGGACAATG CAGGACAGCT AGTGTTTCTG
    GCTACAGAAG GGGACCATCT TCAGTTGTCT GAAGAATGGT TTTATGCCCA CATCATACCA
    TTCCTTGGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu33593
    Clone ID Related Accession (Same CDS sequence) XM_005271008.1 , NM_001363695.1 , NM_001363695.2
    Accession Version NM_001363695.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 849bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-27
    Organism Homo sapiens(human)
    Product palmitoyl-protein thioesterase 1 isoform 3 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL512599.33. On May 25, 2018 this sequence version replaced XM_005271008.2. Transcript Variant: This variant (3) lacks an in-frame exon in the 3' coding region compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.9152.1, SRR3476690.252968.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGCGTCGC CCGGCTGCCT GTGGCTCTTG GCTGTGGCTC TCCTGCCATG GACCTGCGCT 
    TCTCGGGCGC TGCAGCATCT GGACCCGCCG GCGCCGCTGC CGTTGGTGAT CTGGCATGGG
    ATGGGAGACA GCTGTTGCAA TCCCTTAAGC ATGGGTGCTA TTAAAAAAAT GGTGGAGAAG
    AAAATACCTG GAATTTACGT CTTATCTTTA GAGATTGGGA AGACCCTGAT GGAGGACGTG
    GAGAACAGCT TCTTCTTGAA TGTCAATTCC CAAGTAACAA CAGTGTGTCA GGCACTTGCT
    AAGGATCCTA AATTGCAGCA AGGCTACAAT GCTATGGGAT TCTCCCAGGG AGGCCAATTT
    CTGAGGGCAG TGGCTCAGAG ATGCCCTTCA CCTCCCATGA TCAATCTGAT CTCGGTTGGG
    GGACAACATC AAGGTGTTTT TGGACTCCCT CGATGCCCAG GAGAGAGCTC TCACATCTGT
    GACTTCATCC GAAAAACACT GAATGCTGGG GCGTACTCCA AAGTTGTTCA GGAACGCCTC
    GTGCAAGCCG AATACTGGCA TGACCCCATA AAGGAGGATG TGTATCGCAA CCACAGCATC
    TTCTTGGCAG ATATAAATCA GGAGCGGGGT ATCAATGAGT CCTACAAGAA AAACCTGATG
    GCCCTGAAGA AGTTTGTGAT GGTGAAATTC CTCAATGATT CCATTGTGGA CCCTGTAGAT
    TCGGAGGACC GCCTGGGGCT AAAGGAAATG GACAATGCAG GACAGCTAGT GTTTCTGGCT
    ACAGAAGGGG ACCATCTTCA GTTGTCTGAA GAATGGTTTT ATGCCCACAT CATACCATTC
    CTTGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001350624.1
    CDS233..1081
    Translation

    Target ORF information:

    RefSeq Version NM_001363695.1
    Organism Homo sapiens(human)
    Definition Homo sapiens palmitoyl-protein thioesterase 1 (PPT1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001363695.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGCGTCGC CCGGCTGCCT GTGGCTCTTG GCTGTGGCTC TCCTGCCATG GACCTGCGCT 
    TCTCGGGCGC TGCAGCATCT GGACCCGCCG GCGCCGCTGC CGTTGGTGAT CTGGCATGGG
    ATGGGAGACA GCTGTTGCAA TCCCTTAAGC ATGGGTGCTA TTAAAAAAAT GGTGGAGAAG
    AAAATACCTG GAATTTACGT CTTATCTTTA GAGATTGGGA AGACCCTGAT GGAGGACGTG
    GAGAACAGCT TCTTCTTGAA TGTCAATTCC CAAGTAACAA CAGTGTGTCA GGCACTTGCT
    AAGGATCCTA AATTGCAGCA AGGCTACAAT GCTATGGGAT TCTCCCAGGG AGGCCAATTT
    CTGAGGGCAG TGGCTCAGAG ATGCCCTTCA CCTCCCATGA TCAATCTGAT CTCGGTTGGG
    GGACAACATC AAGGTGTTTT TGGACTCCCT CGATGCCCAG GAGAGAGCTC TCACATCTGT
    GACTTCATCC GAAAAACACT GAATGCTGGG GCGTACTCCA AAGTTGTTCA GGAACGCCTC
    GTGCAAGCCG AATACTGGCA TGACCCCATA AAGGAGGATG TGTATCGCAA CCACAGCATC
    TTCTTGGCAG ATATAAATCA GGAGCGGGGT ATCAATGAGT CCTACAAGAA AAACCTGATG
    GCCCTGAAGA AGTTTGTGAT GGTGAAATTC CTCAATGATT CCATTGTGGA CCCTGTAGAT
    TCGGAGGACC GCCTGGGGCT AAAGGAAATG GACAATGCAG GACAGCTAGT GTTTCTGGCT
    ACAGAAGGGG ACCATCTTCA GTTGTCTGAA GAATGGTTTT ATGCCCACAT CATACCATTC
    CTTGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu33593
    Clone ID Related Accession (Same CDS sequence) XM_005271008.1 , NM_001363695.1 , NM_001363695.2
    Accession Version NM_001363695.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 849bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-01
    Organism Homo sapiens(human)
    Product palmitoyl-protein thioesterase 1 isoform 3 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL512599.33. On Jun 2, 2019 this sequence version replaced NM_001363695.1. Transcript Variant: This variant (3) lacks an in-frame exon in the 3' coding region compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.208036.1, SRR1803611.132724.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGCGTCGC CCGGCTGCCT GTGGCTCTTG GCTGTGGCTC TCCTGCCATG GACCTGCGCT 
    TCTCGGGCGC TGCAGCATCT GGACCCGCCG GCGCCGCTGC CGTTGGTGAT CTGGCATGGG
    ATGGGAGACA GCTGTTGCAA TCCCTTAAGC ATGGGTGCTA TTAAAAAAAT GGTGGAGAAG
    AAAATACCTG GAATTTACGT CTTATCTTTA GAGATTGGGA AGACCCTGAT GGAGGACGTG
    GAGAACAGCT TCTTCTTGAA TGTCAATTCC CAAGTAACAA CAGTGTGTCA GGCACTTGCT
    AAGGATCCTA AATTGCAGCA AGGCTACAAT GCTATGGGAT TCTCCCAGGG AGGCCAATTT
    CTGAGGGCAG TGGCTCAGAG ATGCCCTTCA CCTCCCATGA TCAATCTGAT CTCGGTTGGG
    GGACAACATC AAGGTGTTTT TGGACTCCCT CGATGCCCAG GAGAGAGCTC TCACATCTGT
    GACTTCATCC GAAAAACACT GAATGCTGGG GCGTACTCCA AAGTTGTTCA GGAACGCCTC
    GTGCAAGCCG AATACTGGCA TGACCCCATA AAGGAGGATG TGTATCGCAA CCACAGCATC
    TTCTTGGCAG ATATAAATCA GGAGCGGGGT ATCAATGAGT CCTACAAGAA AAACCTGATG
    GCCCTGAAGA AGTTTGTGAT GGTGAAATTC CTCAATGATT CCATTGTGGA CCCTGTAGAT
    TCGGAGGACC GCCTGGGGCT AAAGGAAATG GACAATGCAG GACAGCTAGT GTTTCTGGCT
    ACAGAAGGGG ACCATCTTCA GTTGTCTGAA GAATGGTTTT ATGCCCACAT CATACCATTC
    CTTGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001350624.1
    CDS15..863
    Translation

    Target ORF information:

    RefSeq Version NM_001363695.2
    Organism Homo sapiens(human)
    Definition Homo sapiens palmitoyl-protein thioesterase 1 (PPT1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001363695.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGCGTCGC CCGGCTGCCT GTGGCTCTTG GCTGTGGCTC TCCTGCCATG GACCTGCGCT 
    TCTCGGGCGC TGCAGCATCT GGACCCGCCG GCGCCGCTGC CGTTGGTGAT CTGGCATGGG
    ATGGGAGACA GCTGTTGCAA TCCCTTAAGC ATGGGTGCTA TTAAAAAAAT GGTGGAGAAG
    AAAATACCTG GAATTTACGT CTTATCTTTA GAGATTGGGA AGACCCTGAT GGAGGACGTG
    GAGAACAGCT TCTTCTTGAA TGTCAATTCC CAAGTAACAA CAGTGTGTCA GGCACTTGCT
    AAGGATCCTA AATTGCAGCA AGGCTACAAT GCTATGGGAT TCTCCCAGGG AGGCCAATTT
    CTGAGGGCAG TGGCTCAGAG ATGCCCTTCA CCTCCCATGA TCAATCTGAT CTCGGTTGGG
    GGACAACATC AAGGTGTTTT TGGACTCCCT CGATGCCCAG GAGAGAGCTC TCACATCTGT
    GACTTCATCC GAAAAACACT GAATGCTGGG GCGTACTCCA AAGTTGTTCA GGAACGCCTC
    GTGCAAGCCG AATACTGGCA TGACCCCATA AAGGAGGATG TGTATCGCAA CCACAGCATC
    TTCTTGGCAG ATATAAATCA GGAGCGGGGT ATCAATGAGT CCTACAAGAA AAACCTGATG
    GCCCTGAAGA AGTTTGTGAT GGTGAAATTC CTCAATGATT CCATTGTGGA CCCTGTAGAT
    TCGGAGGACC GCCTGGGGCT AAAGGAAATG GACAATGCAG GACAGCTAGT GTTTCTGGCT
    ACAGAAGGGG ACCATCTTCA GTTGTCTGAA GAATGGTTTT ATGCCCACAT CATACCATTC
    CTTGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu33593
    Clone ID Related Accession (Same CDS sequence) XM_005271008.1 , NM_001363695.1 , NM_001363695.2
    Accession Version XM_005271008.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 849bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product palmitoyl-protein thioesterase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGCGTCGC CCGGCTGCCT GTGGCTCTTG GCTGTGGCTC TCCTGCCATG GACCTGCGCT 
    TCTCGGGCGC TGCAGCATCT GGACCCGCCG GCGCCGCTGC CGTTGGTGAT CTGGCATGGG
    ATGGGAGACA GCTGTTGCAA TCCCTTAAGC ATGGGTGCTA TTAAAAAAAT GGTGGAGAAG
    AAAATACCTG GAATTTACGT CTTATCTTTA GAGATTGGGA AGACCCTGAT GGAGGACGTG
    GAGAACAGCT TCTTCTTGAA TGTCAATTCC CAAGTAACAA CAGTGTGTCA GGCACTTGCT
    AAGGATCCTA AATTGCAGCA AGGCTACAAT GCTATGGGAT TCTCCCAGGG AGGCCAATTT
    CTGAGGGCAG TGGCTCAGAG ATGCCCTTCA CCTCCCATGA TCAATCTGAT CTCGGTTGGG
    GGACAACATC AAGGTGTTTT TGGACTCCCT CGATGCCCAG GAGAGAGCTC TCACATCTGT
    GACTTCATCC GAAAAACACT GAATGCTGGG GCGTACTCCA AAGTTGTTCA GGAACGCCTC
    GTGCAAGCCG AATACTGGCA TGACCCCATA AAGGAGGATG TGTATCGCAA CCACAGCATC
    TTCTTGGCAG ATATAAATCA GGAGCGGGGT ATCAATGAGT CCTACAAGAA AAACCTGATG
    GCCCTGAAGA AGTTTGTGAT GGTGAAATTC CTCAATGATT CCATTGTGGA CCCTGTAGAT
    TCGGAGGACC GCCTGGGGCT AAAGGAAATG GACAATGCAG GACAGCTAGT GTTTCTGGCT
    ACAGAAGGGG ACCATCTTCA GTTGTCTGAA GAATGGTTTT ATGCCCACAT CATACCATTC
    CTTGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005271065.1
    CDS17..865
    Translation

    Target ORF information:

    RefSeq Version XM_005271008.1
    Organism Homo sapiens(human)
    Definition Homo sapiens palmitoyl-protein thioesterase 1 (PPT1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005271008.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGCGTCGC CCGGCTGCCT GTGGCTCTTG GCTGTGGCTC TCCTGCCATG GACCTGCGCT 
    TCTCGGGCGC TGCAGCATCT GGACCCGCCG GCGCCGCTGC CGTTGGTGAT CTGGCATGGG
    ATGGGAGACA GCTGTTGCAA TCCCTTAAGC ATGGGTGCTA TTAAAAAAAT GGTGGAGAAG
    AAAATACCTG GAATTTACGT CTTATCTTTA GAGATTGGGA AGACCCTGAT GGAGGACGTG
    GAGAACAGCT TCTTCTTGAA TGTCAATTCC CAAGTAACAA CAGTGTGTCA GGCACTTGCT
    AAGGATCCTA AATTGCAGCA AGGCTACAAT GCTATGGGAT TCTCCCAGGG AGGCCAATTT
    CTGAGGGCAG TGGCTCAGAG ATGCCCTTCA CCTCCCATGA TCAATCTGAT CTCGGTTGGG
    GGACAACATC AAGGTGTTTT TGGACTCCCT CGATGCCCAG GAGAGAGCTC TCACATCTGT
    GACTTCATCC GAAAAACACT GAATGCTGGG GCGTACTCCA AAGTTGTTCA GGAACGCCTC
    GTGCAAGCCG AATACTGGCA TGACCCCATA AAGGAGGATG TGTATCGCAA CCACAGCATC
    TTCTTGGCAG ATATAAATCA GGAGCGGGGT ATCAATGAGT CCTACAAGAA AAACCTGATG
    GCCCTGAAGA AGTTTGTGAT GGTGAAATTC CTCAATGATT CCATTGTGGA CCCTGTAGAT
    TCGGAGGACC GCCTGGGGCT AAAGGAAATG GACAATGCAG GACAGCTAGT GTTTCTGGCT
    ACAGAAGGGG ACCATCTTCA GTTGTCTGAA GAATGGTTTT ATGCCCACAT CATACCATTC
    CTTGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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