NSUN5 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following NSUN5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NSUN5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu15529 NM_001168347.3
Latest version!
Homo sapiens NOP2/Sun RNA methyltransferase 5 (NSUN5), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu15524 NM_018044.4
Latest version!
Homo sapiens NOP2/Sun RNA methyltransferase 5 (NSUN5), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu15526 NM_148956.4
Latest version!
Homo sapiens NOP2/Sun RNA methyltransferase 5 (NSUN5), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
OHu15538 NM_001168348.2
Latest version!
Homo sapiens NOP2/Sun RNA methyltransferase 5 (NSUN5), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu41659 XM_005277604.5
Latest version!
Homo sapiens NOP2/Sun RNA methyltransferase 5 (NSUN5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
View Old Accession Versions >>
OHu15526 NM_148956.3 Homo sapiens NOP2/Sun RNA methyltransferase 5 (NSUN5), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
OHu15529 NM_001168347.2 Homo sapiens NOP2/Sun RNA methyltransferase 5 (NSUN5), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu41659 XM_005277604.4 Homo sapiens NOP2/Sun RNA methyltransferase family member 5 (NSUN5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu15529
    Clone ID Related Accession (Same CDS sequence) NM_001168347.2 , NM_001168347.3
    Accession Version NM_001168347.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1413bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-30
    Organism Homo sapiens(human)
    Product probable 28S rRNA (cytosine-C(5))-methyltransferase isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB154882.1, AK298221.1, AK126375.1, EC442348.1, BM144254.1 and CX866878.1. On May 31, 2019 this sequence version replaced NM_001168347.2. Transcript Variant: This variant (3) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (3) is longer and has a distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.85692.1, AK298221.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGGGCTGT ATGCTGCAGC TGCAGGCGTG TTGGCCGGCG TGGAGAGCCG CCAGGGCTCT 
    ATCAAGGGGT TGGTGTACTC CAGCAACTTC CAGAACGTGA AGCAGCTGTA CGCGCTGGTG
    TGCGAAACGC AGCGCTACTC CGCCGTGCTG GATGCTGTGA TCGCCAGCGC CGGCCTCCTC
    CGTGCGGAGA AGAAGCTGCG GCCGCACCTG GCCAAGGTGC TAGTGTATGA GTTGTTGTTG
    GGAAAGGGCT TTCGAGGGGG TGGGGGCCGA TGGAAGGCTC TGTTGGGCCG GCACCAGGCG
    AGGCTCAAGG CTGAGTTGGC TCGGCTCAAG GTTCATCGGG GTGTGAGCCG GAATGAGGAC
    CTGTTGGAAG TGGGATCCAG GCCTGGTCCA GCCTCCCAGC TGCCTCGATT TGTGCGTGTG
    AACACTCTCA AGACCTGCTC CGATGATGTA GTTGATTATT TCAAGAGACA AGGTTTCTCC
    TATCAGGGTC GGGCTTCCAG CCTCGATGAC TTACGAGCCC TCAAGGGGAA GCATTTTCTC
    CTGGACCCCT TGATGCCGGA GCTGCTGGTG TTTCCCGCCC AGACAGATCT GCATGAACAC
    CCACTGTACC GGGCCGGACA CCTCATTCTG CAGGACAGGG CCAGCTGTCT CCCAGCCATG
    CTGCTGGACC CCCCGCCAGG CTCCCATGTC ATCGATGCCT GTGCCGCCCC AGGCAATAAG
    ACCAGTCACT TGGCTGCTCT TCTGAAGAAC CAAGGGAAGA TCTTTGCCTT TGACCTGGAT
    GCCAAGCGGC TGGCATCCAT GGCCACGCTG CTGGCCCGGG CTGGCGTCTC TTGCTGTGAA
    CTGGCTGAGG AGGACTTCCT GGCGGTCTCC CCCTCGGATC CACGCTACCA TGAGGTCCAC
    TACATCCTGC TGGATCCTTC CTGCAGTGGC TCGGGTATGC CGAGCAGACA GCTGGAGGAG
    CCCGGGGCAG GCACACCTAG CCCGGTGCGT CTGCATGCCC TGGCAGGGTT CCAGCAGCGA
    GCCCTGTGCC ACGCACTCAC TTTCCCTTCC CTGCAGCGGC TCGTCTACTC CACGTGCTCC
    CTCTGCCAGG AGGAGAATGA AGACGTGGTG CGAGATGCGC TGCAGCAGAA CCCGGGCGCC
    TTCAGGCTAG CTCCCGCCCT GCCTGCCTGG CCCCACCGAG GCCTGAGCAC GTTCCCGGGT
    GCCGAGCACT GCCTCCGGGC CTCCCCTGAG ACCACACTCA GCAGTGGCTT CTTCGTTGCT
    GTAATTGAAC GGGTCGAGGT GCCAAGCCTC ACAGGCCAAA GCATCAGCAC CAGAACGCAC
    ACCCAGCCCA GCCCCAAAGA GAAAGAAGAG ACAGCAAAGA GCCGCAGCCG GTGCTTGCAC
    ACCGCCTTGC ACATAGCAGA GGCTCCGGGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001161819.1
    CDS37..1449
    Translation

    Target ORF information:

    RefSeq Version NM_001168347.3
    Organism Homo sapiens(human)
    Definition Homo sapiens NOP2/Sun RNA methyltransferase 5 (NSUN5), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001168347.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGGGCTGT ATGCTGCAGC TGCAGGCGTG TTGGCCGGCG TGGAGAGCCG CCAGGGCTCT 
    ATCAAGGGGT TGGTGTACTC CAGCAACTTC CAGAACGTGA AGCAGCTGTA CGCGCTGGTG
    TGCGAAACGC AGCGCTACTC CGCCGTGCTG GATGCTGTGA TCGCCAGCGC CGGCCTCCTC
    CGTGCGGAGA AGAAGCTGCG GCCGCACCTG GCCAAGGTGC TAGTGTATGA GTTGTTGTTG
    GGAAAGGGCT TTCGAGGGGG TGGGGGCCGA TGGAAGGCTC TGTTGGGCCG GCACCAGGCG
    AGGCTCAAGG CTGAGTTGGC TCGGCTCAAG GTTCATCGGG GTGTGAGCCG GAATGAGGAC
    CTGTTGGAAG TGGGATCCAG GCCTGGTCCA GCCTCCCAGC TGCCTCGATT TGTGCGTGTG
    AACACTCTCA AGACCTGCTC CGATGATGTA GTTGATTATT TCAAGAGACA AGGTTTCTCC
    TATCAGGGTC GGGCTTCCAG CCTCGATGAC TTACGAGCCC TCAAGGGGAA GCATTTTCTC
    CTGGACCCCT TGATGCCGGA GCTGCTGGTG TTTCCCGCCC AGACAGATCT GCATGAACAC
    CCACTGTACC GGGCCGGACA CCTCATTCTG CAGGACAGGG CCAGCTGTCT CCCAGCCATG
    CTGCTGGACC CCCCGCCAGG CTCCCATGTC ATCGATGCCT GTGCCGCCCC AGGCAATAAG
    ACCAGTCACT TGGCTGCTCT TCTGAAGAAC CAAGGGAAGA TCTTTGCCTT TGACCTGGAT
    GCCAAGCGGC TGGCATCCAT GGCCACGCTG CTGGCCCGGG CTGGCGTCTC TTGCTGTGAA
    CTGGCTGAGG AGGACTTCCT GGCGGTCTCC CCCTCGGATC CACGCTACCA TGAGGTCCAC
    TACATCCTGC TGGATCCTTC CTGCAGTGGC TCGGGTATGC CGAGCAGACA GCTGGAGGAG
    CCCGGGGCAG GCACACCTAG CCCGGTGCGT CTGCATGCCC TGGCAGGGTT CCAGCAGCGA
    GCCCTGTGCC ACGCACTCAC TTTCCCTTCC CTGCAGCGGC TCGTCTACTC CACGTGCTCC
    CTCTGCCAGG AGGAGAATGA AGACGTGGTG CGAGATGCGC TGCAGCAGAA CCCGGGCGCC
    TTCAGGCTAG CTCCCGCCCT GCCTGCCTGG CCCCACCGAG GCCTGAGCAC GTTCCCGGGT
    GCCGAGCACT GCCTCCGGGC CTCCCCTGAG ACCACACTCA GCAGTGGCTT CTTCGTTGCT
    GTAATTGAAC GGGTCGAGGT GCCAAGCCTC ACAGGCCAAA GCATCAGCAC CAGAACGCAC
    ACCCAGCCCA GCCCCAAAGA GAAAGAAGAG ACAGCAAAGA GCCGCAGCCG GTGCTTGCAC
    ACCGCCTTGC ACATAGCAGA GGCTCCGGGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu15524
    Clone ID Related Accession (Same CDS sequence) NM_018044.4
    Accession Version NM_018044.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1290bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-26
    Organism Homo sapiens(human)
    Product probable 28S rRNA (cytosine-C(5))-methyltransferase isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB154882.1, AK001129.1, AK126375.1, EC442348.1, BM144254.1 and CX866878.1. On Jul 25, 2013 this sequence version replaced NM_018044.3. Transcript Variant: This variant (2) includes an alternate segment in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.90888.1, SRR1660803.159219.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGGGCTGT ATGCTGCAGC TGCAGGCGTG TTGGCCGGCG TGGAGAGCCG CCAGGGCTCT 
    ATCAAGGGGT TGGTGTACTC CAGCAACTTC CAGAACGTGA AGCAGCTGTA CGCGCTGGTG
    TGCGAAACGC AGCGCTACTC CGCCGTGCTG GATGCTGTGA TCGCCAGCGC CGGCCTCCTC
    CGTGCGGAGA AGAAGCTGCG GCCGCACCTG GCCAAGGTGC TAGTGTATGA GTTGTTGTTG
    GGAAAGGGCT TTCGAGGGGG TGGGGGCCGA TGGAAGGCTC TGTTGGGCCG GCACCAGGCG
    AGGCTCAAGG CTGAGTTGGC TCGGCTCAAG GTTCATCGGG GTGTGAGCCG GAATGAGGAC
    CTGTTGGAAG TGGGATCCAG GCCTGGTCCA GCCTCCCAGC TGCCTCGATT TGTGCGTGTG
    AACACTCTCA AGACCTGCTC CGATGATGTA GTTGATTATT TCAAGAGACA AGGTTTCTCC
    TATCAGGGTC GGGCTTCCAG CCTCGATGAC TTACGAGCCC TCAAGGGGAA GCATTTTCTC
    CTGGACCCCT TGATGCCGGA GCTGCTGGTG TTTCCCGCCC AGACAGATCT GCATGAACAC
    CCACTGTACC GGGCCGGACA CCTCATTCTG CAGGACAGGG CCAGCTGTCT CCCAGCCATG
    CTGCTGGACC CCCCGCCAGG CTCCCATGTC ATCGATGCCT GTGCCGCCCC AGGCAATAAG
    ACCAGTCACT TGGCTGCTCT TCTGAAGAAC CAAGGGAAGA TCTTTGCCTT TGACCTGGAT
    GCCAAGCGGC TGGCATCCAT GGCCACGCTG CTGGCCCGGG CTGGCGTCTC TTGCTGTGAA
    CTGGCTGAGG AGGACTTCCT GGCGGTCTCC CCCTCGGATC CACGCTACCA TGAGGTCCAC
    TACATCCTGC TGGATCCTTC CTGCAGTGGC TCGGGTATGC CGAGCAGACA GCTGGAGGAG
    CCCGGGGCAG GCACACCTAG CCCGGTGCGT CTGCATGCCC TGGCAGGGTT CCAGCAGCGA
    GCCCTGTGCC ACGCACTCAC TTTCCCTTCC CTGCAGCGGC TCGTCTACTC CACGTGCTCC
    CTCTGCCAGG AGGAGAATGA AGACGTGGTG CGAGATGCGC TGCAGCAGAA CCCGGGCGCC
    TTCAGGCTAG CTCCCGCCCT GCCTGCCTGG CCCCACCGAG GCCTGAGCAC GTTCCCGGGT
    GCCGAGCACT GCCTCCGGGC CTCCCCTGAG ACCACACTCA GCAGTGGCTT CTTCGTTGCT
    GTAATTGAAC GGGTCGAGGT GCCAAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_060514.1
    CDS78..1367
    Translation

    Target ORF information:

    RefSeq Version NM_018044.4
    Organism Homo sapiens(human)
    Definition Homo sapiens NOP2/Sun RNA methyltransferase 5 (NSUN5), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_018044.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGGGCTGT ATGCTGCAGC TGCAGGCGTG TTGGCCGGCG TGGAGAGCCG CCAGGGCTCT 
    ATCAAGGGGT TGGTGTACTC CAGCAACTTC CAGAACGTGA AGCAGCTGTA CGCGCTGGTG
    TGCGAAACGC AGCGCTACTC CGCCGTGCTG GATGCTGTGA TCGCCAGCGC CGGCCTCCTC
    CGTGCGGAGA AGAAGCTGCG GCCGCACCTG GCCAAGGTGC TAGTGTATGA GTTGTTGTTG
    GGAAAGGGCT TTCGAGGGGG TGGGGGCCGA TGGAAGGCTC TGTTGGGCCG GCACCAGGCG
    AGGCTCAAGG CTGAGTTGGC TCGGCTCAAG GTTCATCGGG GTGTGAGCCG GAATGAGGAC
    CTGTTGGAAG TGGGATCCAG GCCTGGTCCA GCCTCCCAGC TGCCTCGATT TGTGCGTGTG
    AACACTCTCA AGACCTGCTC CGATGATGTA GTTGATTATT TCAAGAGACA AGGTTTCTCC
    TATCAGGGTC GGGCTTCCAG CCTCGATGAC TTACGAGCCC TCAAGGGGAA GCATTTTCTC
    CTGGACCCCT TGATGCCGGA GCTGCTGGTG TTTCCCGCCC AGACAGATCT GCATGAACAC
    CCACTGTACC GGGCCGGACA CCTCATTCTG CAGGACAGGG CCAGCTGTCT CCCAGCCATG
    CTGCTGGACC CCCCGCCAGG CTCCCATGTC ATCGATGCCT GTGCCGCCCC AGGCAATAAG
    ACCAGTCACT TGGCTGCTCT TCTGAAGAAC CAAGGGAAGA TCTTTGCCTT TGACCTGGAT
    GCCAAGCGGC TGGCATCCAT GGCCACGCTG CTGGCCCGGG CTGGCGTCTC TTGCTGTGAA
    CTGGCTGAGG AGGACTTCCT GGCGGTCTCC CCCTCGGATC CACGCTACCA TGAGGTCCAC
    TACATCCTGC TGGATCCTTC CTGCAGTGGC TCGGGTATGC CGAGCAGACA GCTGGAGGAG
    CCCGGGGCAG GCACACCTAG CCCGGTGCGT CTGCATGCCC TGGCAGGGTT CCAGCAGCGA
    GCCCTGTGCC ACGCACTCAC TTTCCCTTCC CTGCAGCGGC TCGTCTACTC CACGTGCTCC
    CTCTGCCAGG AGGAGAATGA AGACGTGGTG CGAGATGCGC TGCAGCAGAA CCCGGGCGCC
    TTCAGGCTAG CTCCCGCCCT GCCTGCCTGG CCCCACCGAG GCCTGAGCAC GTTCCCGGGT
    GCCGAGCACT GCCTCCGGGC CTCCCCTGAG ACCACACTCA GCAGTGGCTT CTTCGTTGCT
    GTAATTGAAC GGGTCGAGGT GCCAAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu15526
    Clone ID Related Accession (Same CDS sequence) NM_148956.3 , NM_148956.4
    Accession Version NM_148956.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1401bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-26
    Organism Homo sapiens(human)
    Product probable 28S rRNA (cytosine-C(5))-methyltransferase isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB154882.1, BC008084.2, AK126375.1, EC442348.1, BM144254.1 and CX866878.1. On Jul 25, 2013 this sequence version replaced NM_148956.2. Transcript Variant: This variant (1) encodes isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.241875.1, SRR1660807.214717.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGGGCTGT ATGCTGCAGC TGCAGGCGTG TTGGCCGGCG TGGAGAGCCG CCAGGGCTCT 
    ATCAAGGGGT TGGTGTACTC CAGCAACTTC CAGAACGTGA AGCAGCTGTA CGCGCTGGTG
    TGCGAAACGC AGCGCTACTC CGCCGTGCTG GATGCTGTGA TCGCCAGCGC CGGCCTCCTC
    CGTGCGGAGA AGAAGCTGCG GCCGCACCTG GCCAAGGTGC TAGTGTATGA GTTGTTGTTG
    GGAAAGGGCT TTCGAGGGGG TGGGGGCCGA TGGAAGGCTC TGTTGGGCCG GCACCAGGCG
    AGGCTCAAGG CTGAGTTGGC TCGGCTCAAG GTTCATCGGG GTGTGAGCCG GAATGAGGAC
    CTGTTGGAAG TGGGATCCAG GCCTGGTCCA GCCTCCCAGC TGCCTCGATT TGTGCGTGTG
    AACACTCTCA AGACCTGCTC CGATGATGTA GTTGATTATT TCAAGAGACA AGGTTTCTCC
    TATCAGGGTC GGGCTTCCAG CCTCGATGAC TTACGAGCCC TCAAGGGGAA GCATTTTCTC
    CTGGACCCCT TGATGCCGGA GCTGCTGGTG TTTCCCGCCC AGACAGATCT GCATGAACAC
    CCACTGTACC GGGCCGGACA CCTCATTCTG CAGGACAGGG CCAGCTGTCT CCCAGCCATG
    CTGCTGGACC CCCCGCCAGG CTCCCATGTC ATCGATGCCT GTGCCGCCCC AGGCAATAAG
    ACCAGTCACT TGGCTGCTCT TCTGAAGAAC CAAGGGAAGA TCTTTGCCTT TGACCTGGAT
    GCCAAGCGGC TGGCATCCAT GGCCACGCTG CTGGCCCGGG CTGGCGTCTC TTGCTGTGAA
    CTGGCTGAGG AGGACTTCCT GGCGGTCTCC CCCTCGGATC CACGCTACCA TGAGGTCCAC
    TACATCCTGC TGGATCCTTC CTGCAGTGGC TCGGGTATGC CGAGCAGACA GCTGGAGGAG
    CCCGGGGCAG GCACACCTAG CCCGGTGCGT CTGCATGCCC TGGCAGGGTT CCAGCAGCGA
    GCCCTGTGCC ACGCACTCAC TTTCCCTTCC CTGCAGCGGC TCGTCTACTC CACGTGCTCC
    CTCTGCCAGG AGGAGAATGA AGACGTGGTG CGAGATGCGC TGCAGCAGAA CCCGGGCGCC
    TTCAGGCTAG CTCCCGCCCT GCCTGCCTGG CCCCACCGAG GCCTGAGCAC GTTCCCGGGT
    GCCGAGCACT GCCTCCGGGC CTCCCCTGAG ACCACACTCA GCAGTGGCTT CTTCGTTGCT
    GTAATTGAAC GGGTCGAGGT GCCAAGCTCA GCCTCACAGG CCAAAGCATC AGCACCAGAA
    CGCACACCCA GCCCAGCCCC AAAGAGAAAG AAGAGACAGC AAAGAGCCGC AGCCGGTGCT
    TGCACACCGC CTTGCACATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_683759.1
    CDS78..1478
    Translation

    Target ORF information:

    RefSeq Version NM_148956.3
    Organism Homo sapiens(human)
    Definition Homo sapiens NOP2/Sun RNA methyltransferase 5 (NSUN5), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_148956.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGGGCTGT ATGCTGCAGC TGCAGGCGTG TTGGCCGGCG TGGAGAGCCG CCAGGGCTCT 
    ATCAAGGGGT TGGTGTACTC CAGCAACTTC CAGAACGTGA AGCAGCTGTA CGCGCTGGTG
    TGCGAAACGC AGCGCTACTC CGCCGTGCTG GATGCTGTGA TCGCCAGCGC CGGCCTCCTC
    CGTGCGGAGA AGAAGCTGCG GCCGCACCTG GCCAAGGTGC TAGTGTATGA GTTGTTGTTG
    GGAAAGGGCT TTCGAGGGGG TGGGGGCCGA TGGAAGGCTC TGTTGGGCCG GCACCAGGCG
    AGGCTCAAGG CTGAGTTGGC TCGGCTCAAG GTTCATCGGG GTGTGAGCCG GAATGAGGAC
    CTGTTGGAAG TGGGATCCAG GCCTGGTCCA GCCTCCCAGC TGCCTCGATT TGTGCGTGTG
    AACACTCTCA AGACCTGCTC CGATGATGTA GTTGATTATT TCAAGAGACA AGGTTTCTCC
    TATCAGGGTC GGGCTTCCAG CCTCGATGAC TTACGAGCCC TCAAGGGGAA GCATTTTCTC
    CTGGACCCCT TGATGCCGGA GCTGCTGGTG TTTCCCGCCC AGACAGATCT GCATGAACAC
    CCACTGTACC GGGCCGGACA CCTCATTCTG CAGGACAGGG CCAGCTGTCT CCCAGCCATG
    CTGCTGGACC CCCCGCCAGG CTCCCATGTC ATCGATGCCT GTGCCGCCCC AGGCAATAAG
    ACCAGTCACT TGGCTGCTCT TCTGAAGAAC CAAGGGAAGA TCTTTGCCTT TGACCTGGAT
    GCCAAGCGGC TGGCATCCAT GGCCACGCTG CTGGCCCGGG CTGGCGTCTC TTGCTGTGAA
    CTGGCTGAGG AGGACTTCCT GGCGGTCTCC CCCTCGGATC CACGCTACCA TGAGGTCCAC
    TACATCCTGC TGGATCCTTC CTGCAGTGGC TCGGGTATGC CGAGCAGACA GCTGGAGGAG
    CCCGGGGCAG GCACACCTAG CCCGGTGCGT CTGCATGCCC TGGCAGGGTT CCAGCAGCGA
    GCCCTGTGCC ACGCACTCAC TTTCCCTTCC CTGCAGCGGC TCGTCTACTC CACGTGCTCC
    CTCTGCCAGG AGGAGAATGA AGACGTGGTG CGAGATGCGC TGCAGCAGAA CCCGGGCGCC
    TTCAGGCTAG CTCCCGCCCT GCCTGCCTGG CCCCACCGAG GCCTGAGCAC GTTCCCGGGT
    GCCGAGCACT GCCTCCGGGC CTCCCCTGAG ACCACACTCA GCAGTGGCTT CTTCGTTGCT
    GTAATTGAAC GGGTCGAGGT GCCAAGCTCA GCCTCACAGG CCAAAGCATC AGCACCAGAA
    CGCACACCCA GCCCAGCCCC AAAGAGAAAG AAGAGACAGC AAAGAGCCGC AGCCGGTGCT
    TGCACACCGC CTTGCACATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu15526
    Clone ID Related Accession (Same CDS sequence) NM_148956.3 , NM_148956.4
    Accession Version NM_148956.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1401bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-25
    Organism Homo sapiens(human)
    Product probable 28S rRNA (cytosine-C(5))-methyltransferase isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB154882.1, BC008084.2, AK126375.1, EC442348.1, BM144254.1 and CX866878.1. On May 17, 2019 this sequence version replaced NM_148956.3. Transcript Variant: This variant (1) encodes isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK098619.1, SRR1660807.214717.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000438747.7/ ENSP00000388464.2 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGGGCTGT ATGCTGCAGC TGCAGGCGTG TTGGCCGGCG TGGAGAGCCG CCAGGGCTCT 
    ATCAAGGGGT TGGTGTACTC CAGCAACTTC CAGAACGTGA AGCAGCTGTA CGCGCTGGTG
    TGCGAAACGC AGCGCTACTC CGCCGTGCTG GATGCTGTGA TCGCCAGCGC CGGCCTCCTC
    CGTGCGGAGA AGAAGCTGCG GCCGCACCTG GCCAAGGTGC TAGTGTATGA GTTGTTGTTG
    GGAAAGGGCT TTCGAGGGGG TGGGGGCCGA TGGAAGGCTC TGTTGGGCCG GCACCAGGCG
    AGGCTCAAGG CTGAGTTGGC TCGGCTCAAG GTTCATCGGG GTGTGAGCCG GAATGAGGAC
    CTGTTGGAAG TGGGATCCAG GCCTGGTCCA GCCTCCCAGC TGCCTCGATT TGTGCGTGTG
    AACACTCTCA AGACCTGCTC CGATGATGTA GTTGATTATT TCAAGAGACA AGGTTTCTCC
    TATCAGGGTC GGGCTTCCAG CCTCGATGAC TTACGAGCCC TCAAGGGGAA GCATTTTCTC
    CTGGACCCCT TGATGCCGGA GCTGCTGGTG TTTCCCGCCC AGACAGATCT GCATGAACAC
    CCACTGTACC GGGCCGGACA CCTCATTCTG CAGGACAGGG CCAGCTGTCT CCCAGCCATG
    CTGCTGGACC CCCCGCCAGG CTCCCATGTC ATCGATGCCT GTGCCGCCCC AGGCAATAAG
    ACCAGTCACT TGGCTGCTCT TCTGAAGAAC CAAGGGAAGA TCTTTGCCTT TGACCTGGAT
    GCCAAGCGGC TGGCATCCAT GGCCACGCTG CTGGCCCGGG CTGGCGTCTC TTGCTGTGAA
    CTGGCTGAGG AGGACTTCCT GGCGGTCTCC CCCTCGGATC CACGCTACCA TGAGGTCCAC
    TACATCCTGC TGGATCCTTC CTGCAGTGGC TCGGGTATGC CGAGCAGACA GCTGGAGGAG
    CCCGGGGCAG GCACACCTAG CCCGGTGCGT CTGCATGCCC TGGCAGGGTT CCAGCAGCGA
    GCCCTGTGCC ACGCACTCAC TTTCCCTTCC CTGCAGCGGC TCGTCTACTC CACGTGCTCC
    CTCTGCCAGG AGGAGAATGA AGACGTGGTG CGAGATGCGC TGCAGCAGAA CCCGGGCGCC
    TTCAGGCTAG CTCCCGCCCT GCCTGCCTGG CCCCACCGAG GCCTGAGCAC GTTCCCGGGT
    GCCGAGCACT GCCTCCGGGC CTCCCCTGAG ACCACACTCA GCAGTGGCTT CTTCGTTGCT
    GTAATTGAAC GGGTCGAGGT GCCAAGCTCA GCCTCACAGG CCAAAGCATC AGCACCAGAA
    CGCACACCCA GCCCAGCCCC AAAGAGAAAG AAGAGACAGC AAAGAGCCGC AGCCGGTGCT
    TGCACACCGC CTTGCACATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_683759.1
    CDS37..1437
    Translation

    Target ORF information:

    RefSeq Version NM_148956.4
    Organism Homo sapiens(human)
    Definition Homo sapiens NOP2/Sun RNA methyltransferase 5 (NSUN5), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_148956.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGGGCTGT ATGCTGCAGC TGCAGGCGTG TTGGCCGGCG TGGAGAGCCG CCAGGGCTCT 
    ATCAAGGGGT TGGTGTACTC CAGCAACTTC CAGAACGTGA AGCAGCTGTA CGCGCTGGTG
    TGCGAAACGC AGCGCTACTC CGCCGTGCTG GATGCTGTGA TCGCCAGCGC CGGCCTCCTC
    CGTGCGGAGA AGAAGCTGCG GCCGCACCTG GCCAAGGTGC TAGTGTATGA GTTGTTGTTG
    GGAAAGGGCT TTCGAGGGGG TGGGGGCCGA TGGAAGGCTC TGTTGGGCCG GCACCAGGCG
    AGGCTCAAGG CTGAGTTGGC TCGGCTCAAG GTTCATCGGG GTGTGAGCCG GAATGAGGAC
    CTGTTGGAAG TGGGATCCAG GCCTGGTCCA GCCTCCCAGC TGCCTCGATT TGTGCGTGTG
    AACACTCTCA AGACCTGCTC CGATGATGTA GTTGATTATT TCAAGAGACA AGGTTTCTCC
    TATCAGGGTC GGGCTTCCAG CCTCGATGAC TTACGAGCCC TCAAGGGGAA GCATTTTCTC
    CTGGACCCCT TGATGCCGGA GCTGCTGGTG TTTCCCGCCC AGACAGATCT GCATGAACAC
    CCACTGTACC GGGCCGGACA CCTCATTCTG CAGGACAGGG CCAGCTGTCT CCCAGCCATG
    CTGCTGGACC CCCCGCCAGG CTCCCATGTC ATCGATGCCT GTGCCGCCCC AGGCAATAAG
    ACCAGTCACT TGGCTGCTCT TCTGAAGAAC CAAGGGAAGA TCTTTGCCTT TGACCTGGAT
    GCCAAGCGGC TGGCATCCAT GGCCACGCTG CTGGCCCGGG CTGGCGTCTC TTGCTGTGAA
    CTGGCTGAGG AGGACTTCCT GGCGGTCTCC CCCTCGGATC CACGCTACCA TGAGGTCCAC
    TACATCCTGC TGGATCCTTC CTGCAGTGGC TCGGGTATGC CGAGCAGACA GCTGGAGGAG
    CCCGGGGCAG GCACACCTAG CCCGGTGCGT CTGCATGCCC TGGCAGGGTT CCAGCAGCGA
    GCCCTGTGCC ACGCACTCAC TTTCCCTTCC CTGCAGCGGC TCGTCTACTC CACGTGCTCC
    CTCTGCCAGG AGGAGAATGA AGACGTGGTG CGAGATGCGC TGCAGCAGAA CCCGGGCGCC
    TTCAGGCTAG CTCCCGCCCT GCCTGCCTGG CCCCACCGAG GCCTGAGCAC GTTCCCGGGT
    GCCGAGCACT GCCTCCGGGC CTCCCCTGAG ACCACACTCA GCAGTGGCTT CTTCGTTGCT
    GTAATTGAAC GGGTCGAGGT GCCAAGCTCA GCCTCACAGG CCAAAGCATC AGCACCAGAA
    CGCACACCCA GCCCAGCCCC AAAGAGAAAG AAGAGACAGC AAAGAGCCGC AGCCGGTGCT
    TGCACACCGC CTTGCACATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu15529
    Clone ID Related Accession (Same CDS sequence) NM_001168347.2 , NM_001168347.3
    Accession Version NM_001168347.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1413bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-26
    Organism Homo sapiens(human)
    Product probable 28S rRNA (cytosine-C(5))-methyltransferase isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB154882.1, AK298221.1, AK126375.1, EC442348.1, BM144254.1 and CX866878.1. On Jul 25, 2013 this sequence version replaced NM_001168347.1. Transcript Variant: This variant (3) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (3) is longer and has a distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.85692.1, AK298221.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGGGCTGT ATGCTGCAGC TGCAGGCGTG TTGGCCGGCG TGGAGAGCCG CCAGGGCTCT 
    ATCAAGGGGT TGGTGTACTC CAGCAACTTC CAGAACGTGA AGCAGCTGTA CGCGCTGGTG
    TGCGAAACGC AGCGCTACTC CGCCGTGCTG GATGCTGTGA TCGCCAGCGC CGGCCTCCTC
    CGTGCGGAGA AGAAGCTGCG GCCGCACCTG GCCAAGGTGC TAGTGTATGA GTTGTTGTTG
    GGAAAGGGCT TTCGAGGGGG TGGGGGCCGA TGGAAGGCTC TGTTGGGCCG GCACCAGGCG
    AGGCTCAAGG CTGAGTTGGC TCGGCTCAAG GTTCATCGGG GTGTGAGCCG GAATGAGGAC
    CTGTTGGAAG TGGGATCCAG GCCTGGTCCA GCCTCCCAGC TGCCTCGATT TGTGCGTGTG
    AACACTCTCA AGACCTGCTC CGATGATGTA GTTGATTATT TCAAGAGACA AGGTTTCTCC
    TATCAGGGTC GGGCTTCCAG CCTCGATGAC TTACGAGCCC TCAAGGGGAA GCATTTTCTC
    CTGGACCCCT TGATGCCGGA GCTGCTGGTG TTTCCCGCCC AGACAGATCT GCATGAACAC
    CCACTGTACC GGGCCGGACA CCTCATTCTG CAGGACAGGG CCAGCTGTCT CCCAGCCATG
    CTGCTGGACC CCCCGCCAGG CTCCCATGTC ATCGATGCCT GTGCCGCCCC AGGCAATAAG
    ACCAGTCACT TGGCTGCTCT TCTGAAGAAC CAAGGGAAGA TCTTTGCCTT TGACCTGGAT
    GCCAAGCGGC TGGCATCCAT GGCCACGCTG CTGGCCCGGG CTGGCGTCTC TTGCTGTGAA
    CTGGCTGAGG AGGACTTCCT GGCGGTCTCC CCCTCGGATC CACGCTACCA TGAGGTCCAC
    TACATCCTGC TGGATCCTTC CTGCAGTGGC TCGGGTATGC CGAGCAGACA GCTGGAGGAG
    CCCGGGGCAG GCACACCTAG CCCGGTGCGT CTGCATGCCC TGGCAGGGTT CCAGCAGCGA
    GCCCTGTGCC ACGCACTCAC TTTCCCTTCC CTGCAGCGGC TCGTCTACTC CACGTGCTCC
    CTCTGCCAGG AGGAGAATGA AGACGTGGTG CGAGATGCGC TGCAGCAGAA CCCGGGCGCC
    TTCAGGCTAG CTCCCGCCCT GCCTGCCTGG CCCCACCGAG GCCTGAGCAC GTTCCCGGGT
    GCCGAGCACT GCCTCCGGGC CTCCCCTGAG ACCACACTCA GCAGTGGCTT CTTCGTTGCT
    GTAATTGAAC GGGTCGAGGT GCCAAGCCTC ACAGGCCAAA GCATCAGCAC CAGAACGCAC
    ACCCAGCCCA GCCCCAAAGA GAAAGAAGAG ACAGCAAAGA GCCGCAGCCG GTGCTTGCAC
    ACCGCCTTGC ACATAGCAGA GGCTCCGGGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001161819.1
    CDS78..1490
    Translation

    Target ORF information:

    RefSeq Version NM_001168347.2
    Organism Homo sapiens(human)
    Definition Homo sapiens NOP2/Sun RNA methyltransferase 5 (NSUN5), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001168347.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGGGCTGT ATGCTGCAGC TGCAGGCGTG TTGGCCGGCG TGGAGAGCCG CCAGGGCTCT 
    ATCAAGGGGT TGGTGTACTC CAGCAACTTC CAGAACGTGA AGCAGCTGTA CGCGCTGGTG
    TGCGAAACGC AGCGCTACTC CGCCGTGCTG GATGCTGTGA TCGCCAGCGC CGGCCTCCTC
    CGTGCGGAGA AGAAGCTGCG GCCGCACCTG GCCAAGGTGC TAGTGTATGA GTTGTTGTTG
    GGAAAGGGCT TTCGAGGGGG TGGGGGCCGA TGGAAGGCTC TGTTGGGCCG GCACCAGGCG
    AGGCTCAAGG CTGAGTTGGC TCGGCTCAAG GTTCATCGGG GTGTGAGCCG GAATGAGGAC
    CTGTTGGAAG TGGGATCCAG GCCTGGTCCA GCCTCCCAGC TGCCTCGATT TGTGCGTGTG
    AACACTCTCA AGACCTGCTC CGATGATGTA GTTGATTATT TCAAGAGACA AGGTTTCTCC
    TATCAGGGTC GGGCTTCCAG CCTCGATGAC TTACGAGCCC TCAAGGGGAA GCATTTTCTC
    CTGGACCCCT TGATGCCGGA GCTGCTGGTG TTTCCCGCCC AGACAGATCT GCATGAACAC
    CCACTGTACC GGGCCGGACA CCTCATTCTG CAGGACAGGG CCAGCTGTCT CCCAGCCATG
    CTGCTGGACC CCCCGCCAGG CTCCCATGTC ATCGATGCCT GTGCCGCCCC AGGCAATAAG
    ACCAGTCACT TGGCTGCTCT TCTGAAGAAC CAAGGGAAGA TCTTTGCCTT TGACCTGGAT
    GCCAAGCGGC TGGCATCCAT GGCCACGCTG CTGGCCCGGG CTGGCGTCTC TTGCTGTGAA
    CTGGCTGAGG AGGACTTCCT GGCGGTCTCC CCCTCGGATC CACGCTACCA TGAGGTCCAC
    TACATCCTGC TGGATCCTTC CTGCAGTGGC TCGGGTATGC CGAGCAGACA GCTGGAGGAG
    CCCGGGGCAG GCACACCTAG CCCGGTGCGT CTGCATGCCC TGGCAGGGTT CCAGCAGCGA
    GCCCTGTGCC ACGCACTCAC TTTCCCTTCC CTGCAGCGGC TCGTCTACTC CACGTGCTCC
    CTCTGCCAGG AGGAGAATGA AGACGTGGTG CGAGATGCGC TGCAGCAGAA CCCGGGCGCC
    TTCAGGCTAG CTCCCGCCCT GCCTGCCTGG CCCCACCGAG GCCTGAGCAC GTTCCCGGGT
    GCCGAGCACT GCCTCCGGGC CTCCCCTGAG ACCACACTCA GCAGTGGCTT CTTCGTTGCT
    GTAATTGAAC GGGTCGAGGT GCCAAGCCTC ACAGGCCAAA GCATCAGCAC CAGAACGCAC
    ACCCAGCCCA GCCCCAAAGA GAAAGAAGAG ACAGCAAAGA GCCGCAGCCG GTGCTTGCAC
    ACCGCCTTGC ACATAGCAGA GGCTCCGGGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu15538
    Clone ID Related Accession (Same CDS sequence) NM_001168348.2
    Accession Version NM_001168348.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1176bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-26
    Organism Homo sapiens(human)
    Product probable 28S rRNA (cytosine-C(5))-methyltransferase isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB154882.1, AK126375.1, AK001129.1, EC442348.1, BM144254.1 and CX866878.1. On Jul 25, 2013 this sequence version replaced NM_001168348.1. Transcript Variant: This variant (4) uses an alternate in-frame splice site in the 5' coding region and includes an alternate segment in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (4) is shorter and has a distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK126375.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGGGCTGT ATGCTGCAGC TGCAGGCGTG TTGGCCGGCG TGGAGAGCCG CCAGGGCTCT 
    ATCAAGGGGT TGGTGTACTC CAGCAACTTC CAGAACGTGA AGCAGCTGTA CGCGCTGGTG
    TGCGAAACGC AGCGCTACTC CGCCGTGCTG GATGCTGTGA TCGCCAGCGC CGGCCTCCTC
    CGTGCGGAGA AGAAGCTGCG GCCGCACCTG GCCAAGGTTC ATCGGGGTGT GAGCCGGAAT
    GAGGACCTGT TGGAAGTGGG ATCCAGGCCT GGTCCAGCCT CCCAGCTGCC TCGATTTGTG
    CGTGTGAACA CTCTCAAGAC CTGCTCCGAT GATGTAGTTG ATTATTTCAA GAGACAAGGT
    TTCTCCTATC AGGGTCGGGC TTCCAGCCTC GATGACTTAC GAGCCCTCAA GGGGAAGCAT
    TTTCTCCTGG ACCCCTTGAT GCCGGAGCTG CTGGTGTTTC CCGCCCAGAC AGATCTGCAT
    GAACACCCAC TGTACCGGGC CGGACACCTC ATTCTGCAGG ACAGGGCCAG CTGTCTCCCA
    GCCATGCTGC TGGACCCCCC GCCAGGCTCC CATGTCATCG ATGCCTGTGC CGCCCCAGGC
    AATAAGACCA GTCACTTGGC TGCTCTTCTG AAGAACCAAG GGAAGATCTT TGCCTTTGAC
    CTGGATGCCA AGCGGCTGGC ATCCATGGCC ACGCTGCTGG CCCGGGCTGG CGTCTCTTGC
    TGTGAACTGG CTGAGGAGGA CTTCCTGGCG GTCTCCCCCT CGGATCCACG CTACCATGAG
    GTCCACTACA TCCTGCTGGA TCCTTCCTGC AGTGGCTCGG GTATGCCGAG CAGACAGCTG
    GAGGAGCCCG GGGCAGGCAC ACCTAGCCCG GTGCGTCTGC ATGCCCTGGC AGGGTTCCAG
    CAGCGAGCCC TGTGCCACGC ACTCACTTTC CCTTCCCTGC AGCGGCTCGT CTACTCCACG
    TGCTCCCTCT GCCAGGAGGA GAATGAAGAC GTGGTGCGAG ATGCGCTGCA GCAGAACCCG
    GGCGCCTTCA GGCTAGCTCC CGCCCTGCCT GCCTGGCCCC ACCGAGGCCT GAGCACGTTC
    CCGGGTGCCG AGCACTGCCT CCGGGCCTCC CCTGAGACCA CACTCAGCAG TGGCTTCTTC
    GTTGCTGTAA TTGAACGGGT CGAGGTGCCA AGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001161820.1
    CDS78..1253
    Translation

    Target ORF information:

    RefSeq Version NM_001168348.2
    Organism Homo sapiens(human)
    Definition Homo sapiens NOP2/Sun RNA methyltransferase 5 (NSUN5), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001168348.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGGGCTGT ATGCTGCAGC TGCAGGCGTG TTGGCCGGCG TGGAGAGCCG CCAGGGCTCT 
    ATCAAGGGGT TGGTGTACTC CAGCAACTTC CAGAACGTGA AGCAGCTGTA CGCGCTGGTG
    TGCGAAACGC AGCGCTACTC CGCCGTGCTG GATGCTGTGA TCGCCAGCGC CGGCCTCCTC
    CGTGCGGAGA AGAAGCTGCG GCCGCACCTG GCCAAGGTTC ATCGGGGTGT GAGCCGGAAT
    GAGGACCTGT TGGAAGTGGG ATCCAGGCCT GGTCCAGCCT CCCAGCTGCC TCGATTTGTG
    CGTGTGAACA CTCTCAAGAC CTGCTCCGAT GATGTAGTTG ATTATTTCAA GAGACAAGGT
    TTCTCCTATC AGGGTCGGGC TTCCAGCCTC GATGACTTAC GAGCCCTCAA GGGGAAGCAT
    TTTCTCCTGG ACCCCTTGAT GCCGGAGCTG CTGGTGTTTC CCGCCCAGAC AGATCTGCAT
    GAACACCCAC TGTACCGGGC CGGACACCTC ATTCTGCAGG ACAGGGCCAG CTGTCTCCCA
    GCCATGCTGC TGGACCCCCC GCCAGGCTCC CATGTCATCG ATGCCTGTGC CGCCCCAGGC
    AATAAGACCA GTCACTTGGC TGCTCTTCTG AAGAACCAAG GGAAGATCTT TGCCTTTGAC
    CTGGATGCCA AGCGGCTGGC ATCCATGGCC ACGCTGCTGG CCCGGGCTGG CGTCTCTTGC
    TGTGAACTGG CTGAGGAGGA CTTCCTGGCG GTCTCCCCCT CGGATCCACG CTACCATGAG
    GTCCACTACA TCCTGCTGGA TCCTTCCTGC AGTGGCTCGG GTATGCCGAG CAGACAGCTG
    GAGGAGCCCG GGGCAGGCAC ACCTAGCCCG GTGCGTCTGC ATGCCCTGGC AGGGTTCCAG
    CAGCGAGCCC TGTGCCACGC ACTCACTTTC CCTTCCCTGC AGCGGCTCGT CTACTCCACG
    TGCTCCCTCT GCCAGGAGGA GAATGAAGAC GTGGTGCGAG ATGCGCTGCA GCAGAACCCG
    GGCGCCTTCA GGCTAGCTCC CGCCCTGCCT GCCTGGCCCC ACCGAGGCCT GAGCACGTTC
    CCGGGTGCCG AGCACTGCCT CCGGGCCTCC CCTGAGACCA CACTCAGCAG TGGCTTCTTC
    GTTGCTGTAA TTGAACGGGT CGAGGTGCCA AGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu41659
    Clone ID Related Accession (Same CDS sequence) XM_005277604.5 , XM_005277604.4
    Accession Version XM_005277604.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1146bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product probable 28S rRNA (cytosine-C(5))-methyltransferase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005277604.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGGGCTGT ATGCTGCAGC TGCAGGCGTG TTGGCCGGCG TGGAGAGCCG CCAGGGCTCT 
    ATCAAGGGGT TGGTGTACTC CAGCAACTTC CAGAACGTGA AGCAGCTGTA CGCGCTGGTG
    TGCGAAACGC AGCGCTACTC CGCCGTGCTG GATGCTGTGA TCGCCAGCGC CGGCCTCCTC
    CGTGCGGAGA AGAAGCTGCG GCCGCACCTG GCCAAGGTGC TAGTGTATGA GTTGTTGTTG
    GGAAAGGGCT TTCGAGGGGG TGGGGGCCGA TGGAAGGCTC TGTTGGGCCG GCACCAGGCG
    AGGCTCAAGG CTGAGTTGGC TCGGCTCAAG GTTCATCGGG GTGTGAGCCG GAATGAGGAC
    CTGTTGGAAG TGGGATCCAG GCCTGGTCCA GCCTCCCAGC TGCCTCGATT TGTGCGTGTG
    AACACTCTCA AGACCTGCTC CGATGATGTA GTTGATTATT TCAAGAGACA AGGTTTCTCC
    TATCAGGGTC GGGCTTCCAG CCTCGATGAC TTACGAGCCC TCAAGGGGAA GCATTTTCTC
    CTGGACCCCT TGATGCCGGA GCTGCTGGTG TTTCCCGCCC AGACAGATCT GCATGAACAC
    CCACTGTACC GGGCCGGACA CCTCATTCTG CAGGACAGGG CCAGCTGTCT CCCAGCCATG
    CTGCTGGACC CCCCGCCAGG CTCCCATGTC ATCGATGCCT GTGCCGCCCC AGGCAATAAG
    ACCAGTCACT TGGCTGCTCT TCTGAAGAAC CAAGGATCTT TGCCTTTGAC CTGGATGCCA
    AGCGGCTGGC ATCCATGGCC ACGCTGCTGG CCCGGGCTGG CGTCTCTTGC TGTGAACTGG
    CTGAGGAGGA CTTCCTGGCG GTCTCCCCCT CGGATCCACG CTACCATGAG GTCCACTACA
    TCCTGCTGGA TCCTTCCTGC AGTGGCTCGG GTATGCCGAG CAGACAGCTG GAGGAGCCCG
    GGGCAGGCAC ACCTAGCCCG GTGCGTCTGC ATGCCCTGGC AGGGTTCCAG CAGCGAGCCC
    TGTGCCACGC ACTCACTTTC CCTTCCCTGC AGCGGCTCGT CTACTCCACG TGCTCCCTCT
    GCCAGGAGGA GAATGAAGAC GTGGTGCGAG ATGCGCTGCA GCAGAACCCG GGCGCCTTCA
    GGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005277661.1
    CDS37..1182
    Translation

    Target ORF information:

    RefSeq Version XM_005277604.5
    Organism Homo sapiens(human)
    Definition Homo sapiens NOP2/Sun RNA methyltransferase 5 (NSUN5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005277604.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGGGCTGT ATGCTGCAGC TGCAGGCGTG TTGGCCGGCG TGGAGAGCCG CCAGGGCTCT 
    ATCAAGGGGT TGGTGTACTC CAGCAACTTC CAGAACGTGA AGCAGCTGTA CGCGCTGGTG
    TGCGAAACGC AGCGCTACTC CGCCGTGCTG GATGCTGTGA TCGCCAGCGC CGGCCTCCTC
    CGTGCGGAGA AGAAGCTGCG GCCGCACCTG GCCAAGGTGC TAGTGTATGA GTTGTTGTTG
    GGAAAGGGCT TTCGAGGGGG TGGGGGCCGA TGGAAGGCTC TGTTGGGCCG GCACCAGGCG
    AGGCTCAAGG CTGAGTTGGC TCGGCTCAAG GTTCATCGGG GTGTGAGCCG GAATGAGGAC
    CTGTTGGAAG TGGGATCCAG GCCTGGTCCA GCCTCCCAGC TGCCTCGATT TGTGCGTGTG
    AACACTCTCA AGACCTGCTC CGATGATGTA GTTGATTATT TCAAGAGACA AGGTTTCTCC
    TATCAGGGTC GGGCTTCCAG CCTCGATGAC TTACGAGCCC TCAAGGGGAA GCATTTTCTC
    CTGGACCCCT TGATGCCGGA GCTGCTGGTG TTTCCCGCCC AGACAGATCT GCATGAACAC
    CCACTGTACC GGGCCGGACA CCTCATTCTG CAGGACAGGG CCAGCTGTCT CCCAGCCATG
    CTGCTGGACC CCCCGCCAGG CTCCCATGTC ATCGATGCCT GTGCCGCCCC AGGCAATAAG
    ACCAGTCACT TGGCTGCTCT TCTGAAGAAC CAAGGATCTT TGCCTTTGAC CTGGATGCCA
    AGCGGCTGGC ATCCATGGCC ACGCTGCTGG CCCGGGCTGG CGTCTCTTGC TGTGAACTGG
    CTGAGGAGGA CTTCCTGGCG GTCTCCCCCT CGGATCCACG CTACCATGAG GTCCACTACA
    TCCTGCTGGA TCCTTCCTGC AGTGGCTCGG GTATGCCGAG CAGACAGCTG GAGGAGCCCG
    GGGCAGGCAC ACCTAGCCCG GTGCGTCTGC ATGCCCTGGC AGGGTTCCAG CAGCGAGCCC
    TGTGCCACGC ACTCACTTTC CCTTCCCTGC AGCGGCTCGT CTACTCCACG TGCTCCCTCT
    GCCAGGAGGA GAATGAAGAC GTGGTGCGAG ATGCGCTGCA GCAGAACCCG GGCGCCTTCA
    GGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu41659
    Clone ID Related Accession (Same CDS sequence) XM_005277604.5 , XM_005277604.4
    Accession Version XM_005277604.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1146bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product probable 28S rRNA (cytosine-C(5))-methyltransferase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005277604.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGGGCTGT ATGCTGCAGC TGCAGGCGTG TTGGCCGGCG TGGAGAGCCG CCAGGGCTCT 
    ATCAAGGGGT TGGTGTACTC CAGCAACTTC CAGAACGTGA AGCAGCTGTA CGCGCTGGTG
    TGCGAAACGC AGCGCTACTC CGCCGTGCTG GATGCTGTGA TCGCCAGCGC CGGCCTCCTC
    CGTGCGGAGA AGAAGCTGCG GCCGCACCTG GCCAAGGTGC TAGTGTATGA GTTGTTGTTG
    GGAAAGGGCT TTCGAGGGGG TGGGGGCCGA TGGAAGGCTC TGTTGGGCCG GCACCAGGCG
    AGGCTCAAGG CTGAGTTGGC TCGGCTCAAG GTTCATCGGG GTGTGAGCCG GAATGAGGAC
    CTGTTGGAAG TGGGATCCAG GCCTGGTCCA GCCTCCCAGC TGCCTCGATT TGTGCGTGTG
    AACACTCTCA AGACCTGCTC CGATGATGTA GTTGATTATT TCAAGAGACA AGGTTTCTCC
    TATCAGGGTC GGGCTTCCAG CCTCGATGAC TTACGAGCCC TCAAGGGGAA GCATTTTCTC
    CTGGACCCCT TGATGCCGGA GCTGCTGGTG TTTCCCGCCC AGACAGATCT GCATGAACAC
    CCACTGTACC GGGCCGGACA CCTCATTCTG CAGGACAGGG CCAGCTGTCT CCCAGCCATG
    CTGCTGGACC CCCCGCCAGG CTCCCATGTC ATCGATGCCT GTGCCGCCCC AGGCAATAAG
    ACCAGTCACT TGGCTGCTCT TCTGAAGAAC CAAGGATCTT TGCCTTTGAC CTGGATGCCA
    AGCGGCTGGC ATCCATGGCC ACGCTGCTGG CCCGGGCTGG CGTCTCTTGC TGTGAACTGG
    CTGAGGAGGA CTTCCTGGCG GTCTCCCCCT CGGATCCACG CTACCATGAG GTCCACTACA
    TCCTGCTGGA TCCTTCCTGC AGTGGCTCGG GTATGCCGAG CAGACAGCTG GAGGAGCCCG
    GGGCAGGCAC ACCTAGCCCG GTGCGTCTGC ATGCCCTGGC AGGGTTCCAG CAGCGAGCCC
    TGTGCCACGC ACTCACTTTC CCTTCCCTGC AGCGGCTCGT CTACTCCACG TGCTCCCTCT
    GCCAGGAGGA GAATGAAGAC GTGGTGCGAG ATGCGCTGCA GCAGAACCCG GGCGCCTTCA
    GGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005277661.1
    CDS55..1200
    Translation

    Target ORF information:

    RefSeq Version XM_005277604.4
    Organism Homo sapiens(human)
    Definition Homo sapiens NOP2/Sun RNA methyltransferase family member 5 (NSUN5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005277604.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGGGCTGT ATGCTGCAGC TGCAGGCGTG TTGGCCGGCG TGGAGAGCCG CCAGGGCTCT 
    ATCAAGGGGT TGGTGTACTC CAGCAACTTC CAGAACGTGA AGCAGCTGTA CGCGCTGGTG
    TGCGAAACGC AGCGCTACTC CGCCGTGCTG GATGCTGTGA TCGCCAGCGC CGGCCTCCTC
    CGTGCGGAGA AGAAGCTGCG GCCGCACCTG GCCAAGGTGC TAGTGTATGA GTTGTTGTTG
    GGAAAGGGCT TTCGAGGGGG TGGGGGCCGA TGGAAGGCTC TGTTGGGCCG GCACCAGGCG
    AGGCTCAAGG CTGAGTTGGC TCGGCTCAAG GTTCATCGGG GTGTGAGCCG GAATGAGGAC
    CTGTTGGAAG TGGGATCCAG GCCTGGTCCA GCCTCCCAGC TGCCTCGATT TGTGCGTGTG
    AACACTCTCA AGACCTGCTC CGATGATGTA GTTGATTATT TCAAGAGACA AGGTTTCTCC
    TATCAGGGTC GGGCTTCCAG CCTCGATGAC TTACGAGCCC TCAAGGGGAA GCATTTTCTC
    CTGGACCCCT TGATGCCGGA GCTGCTGGTG TTTCCCGCCC AGACAGATCT GCATGAACAC
    CCACTGTACC GGGCCGGACA CCTCATTCTG CAGGACAGGG CCAGCTGTCT CCCAGCCATG
    CTGCTGGACC CCCCGCCAGG CTCCCATGTC ATCGATGCCT GTGCCGCCCC AGGCAATAAG
    ACCAGTCACT TGGCTGCTCT TCTGAAGAAC CAAGGATCTT TGCCTTTGAC CTGGATGCCA
    AGCGGCTGGC ATCCATGGCC ACGCTGCTGG CCCGGGCTGG CGTCTCTTGC TGTGAACTGG
    CTGAGGAGGA CTTCCTGGCG GTCTCCCCCT CGGATCCACG CTACCATGAG GTCCACTACA
    TCCTGCTGGA TCCTTCCTGC AGTGGCTCGG GTATGCCGAG CAGACAGCTG GAGGAGCCCG
    GGGCAGGCAC ACCTAGCCCG GTGCGTCTGC ATGCCCTGGC AGGGTTCCAG CAGCGAGCCC
    TGTGCCACGC ACTCACTTTC CCTTCCCTGC AGCGGCTCGT CTACTCCACG TGCTCCCTCT
    GCCAGGAGGA GAATGAAGAC GTGGTGCGAG ATGCGCTGCA GCAGAACCCG GGCGCCTTCA
    GGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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