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POLR3B cDNA ORF clone, Homo sapiens(Human)

Gene Symbol POLR3B
Entrez Gene ID 55703
Full Name RNA polymerase III subunit B
Synonyms C128,HLD8,INMAP,RPC2
General protein information
Preferred Names

RNA polymerase III subunit B

Names

DNA-directed RNA polymerase III subunit RPC2
DNA-directed RNA polymerase III 127.6 kDa polypeptide
DNA-directed RNA polymerase III subunit B
RNA polymerase III subunit C2
interphase nucleus and mitotic apparatus associated protein
polymerase (RNA) III (DNA directed) polypeptide B
polymerase (RNA) III subunit B

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

12

12q23.3

Summary This gene encodes the second largest subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The largest subunit and the encoded protein form the catalytic center of RNA polymerase III. Mutations in this gene are a cause of hypomyelinating leukodystrophy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011].
Disorder MIM:

614366


mRNA and Protein(s)

mRNA Protein Name
NM_001160708.1 NP_001154180.1 DNA-directed RNA polymerase III subunit RPC2 isoform 2
NM_018082.5 NP_060552.4 DNA-directed RNA polymerase III subunit RPC2 isoform 1
XM_017019621.1 XP_016875110.1 DNA-directed RNA polymerase III subunit RPC2 isoform X1



Drosophila melanogaster (fruit fly) RpIII128 NP_523706.1
Kluyveromyces lactis KLLA0F01078g XP_455128.1
Schizosaccharomyces pombe (fission yeast) rpc2 NP_593690.1
Mus musculus (house mouse) Polr3b NP_081699.1
Rattus norvegicus (Norway rat) Polr3b NP_001178807.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP008151 XP_317312.4
Neurospora crassa NCU01772 XP_957195.1
Oryza sativa (rice) Os03g0403100 NP_001050323.1
Homo sapiens (human) POLR3B NP_060552.4
Gallus gallus (chicken) POLR3B XP_416307.3
Danio rerio (zebrafish) polr3b XP_690811.2
Magnaporthe oryzae (rice blast fungus) MGG_06984 XP_003709735.1
Xenopus tropicalis (tropical clawed frog) polr3b NP_001121521.1
Macaca mulatta (rhesus monkey) POLR3B XP_001099515.1
Pan troglodytes (chimpanzee) POLR3B XP_001161567.1
Canis lupus familiaris (dog) POLR3B XP_531760.2
Bos taurus (cattle) POLR3B NP_001179718.1
Caenorhabditis elegans (roundworm) F09F7.3 NP_498192.2
Saccharomyces cerevisiae (baker's yeast) RET1 NP_014850.1
Eremothecium gossypii AGOS_ADL275C NP_983821.2
Arabidopsis thaliana (thale cress) NRPC2 NP_199327.4


Related articles in PubMed

Diffuse hypomyelination is not obligate for POLR3-related disorders.
La Piana R, Cayami FK, Tran LT, Guerrero K, van Spaendonk R, Õunap K, Pajusalu S, Haack T, Wassmer E, Timmann D, Mierzewska H, Poll-Thé BT, Patel C, Cox H, Atik T, Onay H, Ozkınay F, Vanderver A, van der Knaap MS, Wolf NI, Bernard G
Neurology86(17)1622-6(2016 Apr)

Large exonic deletions in POLR3B gene cause POLR3-related leukodystrophy.
Gutierrez M, Thiffault I, Guerrero K, Martos-Moreno GÁ, Tran LT, Benko W, van der Knaap MS, van Spaendonk RM, Wolf NI, Bernard G
Orphanet journal of rare diseases1069(2015 Jun)

Recessive mutations in POLR3B, encoding the second largest subunit of Pol III, cause a rare hypomyelinating leukodystrophy.
Tétreault M, Choquet K, Orcesi S, Tonduti D, Balottin U, Teichmann M, Fribourg S, Schiffmann R, Brais B, Vanderver A, Bernard G
American journal of human genetics89(5)652-5(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Multicenter retrospective study to collect neuroradiologic, clinical, and molecular data of patients with mutations in POLR3A and POLR3B without the classic MRI phenotype: diffuse hypomyelination is not an obligatory feature of POLR3-related disorders; two distinct patterns, selective involvement of the corticospinal tracts and cerebellar atrophy, are added to the MRI presentation of POLR3-related disorders
Title: Diffuse hypomyelination is not obligate for POLR3-related disorders.

first reports of long deletions causing POLR3-related leukodystrophy, suggesting that deletions and duplications in POLR3A or POLR3B should be investigated in patients with a compatible phenotype
Title: Large exonic deletions in POLR3B gene cause POLR3-related leukodystrophy.

Mutations in POLR3A or POLR3B are rare in patients with unclassified hypomyelination.
Title: POLR3A and POLR3B Mutations in Unclassified Hypomyelination.

These results suggest that INMAP might function through p53/p21 pathways.
Title: INMAP overexpression inhibits cell proliferation, induces genomic instability and functions through p53/p21 pathways.

Most patients with 4H leukodystrophy carried the common c.1568T>A POLR3B mutation on one allele.
Title: Clinical spectrum of 4H leukodystrophy caused by POLR3A and POLR3B mutations.

The following POLR3B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLR3B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu22699 NM_001160708.1
Latest version!
Homo sapiens RNA polymerase III subunit B (POLR3B), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$399.50-$559.30
$799.00
OHu27279 NM_018082.5
Latest version!
Homo sapiens RNA polymerase III subunit B (POLR3B), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$399.50-$559.30
$799.00
OHu83968 XM_017019621.1
Latest version!
Homo sapiens polymerase (RNA) III subunit B (POLR3B), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $559.30
$799.00

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2-4 Clone 40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu22699
Clone ID Related Accession (Same CDS sequence) NM_001160708.1
Accession Version NM_001160708.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3228bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Homo sapiens(Human)
Product DNA-directed RNA polymerase III subunit RPC2 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB094040.1, AK122713.1, AC078992.23 and BC033724.2. Transcript Variant: This variant (2) differs in the 5' UTR and initiates translation at an in-frame downstream AUG, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK122713.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2145240 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGAAAGCCA ATGAAAAGGT TACAAGTGAC GCTGACCCTA TGTGGTACTT AAAATATCTT 
AATATCTATG TTGGGCTTCC TGATGTTGAA GAAAGCTTCA ATGTAACTAG ACCAGTGTCC
CCTCATGAGT GCCGTTTGAG AGACATGACA TACTCTGCCC CTATTACAGT GGATATTGAA
TATACCCGAG GCAGCCAGAG GATCATCCGC AATGCCTTAC CTATCGGCAG AATGCCCATA
ATGCTACGTA GTTCAAACTG TGTTCTTACA GGAAAAACGC CAGCAGAATT TGCCAAACTG
AACGAATGTC CCTTAGATCC AGGTGGCTAC TTCATTGTTA AAGGAGTAGA AAAAGTTATT
CTTATCCAAG AGCAGCTGTC TAAGAACAGG ATCATCGTGG AGGCTGATAG AAAAGGGGCT
GTTGGAGCTT CAGTTACCAG CTCTACCCAT GAGAAAAAAA GCAGAACCAA TATGGCTGTG
AAACAAGGAC GATTTTATTT GAGGCATAAT ACTTTGTCAG AAGATATACC CATTGTCATC
ATATTTAAGG CCATGGGTGT TGAGAGTGAC CAGGAAATTG TGCAGATGAT TGGAACAGAG
GAGCACGTGA TGGCTGCATT TGGGCCCAGT CTGGAAGAGT GCCAGAAAGC TCAGATTTTC
ACACAGATGC AGGCATTAAA ATATATAGGG AACAAAGTAA GAAGGCAAAG GATGTGGGGA
GGTGGACCAA AGAAAACCAA AATAGAAGAA GCAAGAGAGC TCCTGGCTTC CACCATTCTG
ACCCATGTCC CAGTTAAGGA ATTCAATTTC CGAGCCAAAT GTATCTATAC TGCAGTGATG
GTGCGAAGAG TTATTCTGGC CCAAGGAGAT AATAAAGTTG ACGACAGAGA TTATTATGGT
AACAAGCGAC TGGAATTGGC AGGACAGCTT TTATCTCTTC TTTTTGAAGA CTTGTTCAAA
AAATTTAATT CTGAAATGAA AAAGATTGCC GACCAGGTGA TTCCTAAGCA AAGAGCAGCC
CAGTTTGATG TTGTCAAACA CATGCGCCAA GACCAGATCA CCAATGGCAT GGTGAATGCT
ATTTCTACCG GAAATTGGTC TTTAAAGAGA TTTAAAATGG ACCGCCAGGG TGTAACCCAA
GTGCTGTCTC GCTTGTCATA TATATCCGCA CTGGGCATGA TGACAAGAAT CTCTTCCCAG
TTTGAAAAAA CGAGAAAAGT GAGTGGTCCT CGCTCCCTCC AGCCATCTCA GTGGGGAATG
CTGTGTCCTT CGGACACTCC TGAAGGAGAG GCATGTGGTT TGGTTAAAAA CTTGGCCCTT
ATGACACACA TCACAACTGA TATGGAAGAT GGACCCATTG TTAAATTAGC CAGTAACTTG
GGAGTAGAAG ATGTGAATTT ATTATGTGGG GAAGAGCTCT CTTACCCAAA TGTGTTTCTT
GTCTTTCTTA ATGGTAACAT CTTAGGTGTC ATTCGAGACC ACAAAAAGCT AGTGAATACA
TTTCGACTCA TGAGAAGAGC AGGATATATC AATGAATTTG TTTCCATCTC AACAAATCTT
ACAGATCGAT GTGTCTATAT TTCTTCTGAT GGGGGAAGGC TATGCAGACC CTACATAATT
GTCAAGAAAC AGAAGCCAGC AGTCACAAAT AAACATATGG AAGAGCTGGC CCAAGGGTAC
AGGAATTTTG AAGATTTCTT ACATGAGAGT CTGGTTGAAT ATTTAGATGT GAATGAAGAA
AATGATTGTA ACATTGCACT GTACGAACAC ACAATTAATA AAGACACCAC CCACTTGGAG
ATTGAACCCT TCACTCTTCT CGGCGTGTGT GCTGGACTTA TCCCATACCC TCACCATAAC
CAGTCACCGA GAAACACTTA TCAGTGTGCC ATGGGGAAAC AAGCCATGGG TACTATAGGA
TACAACCAGC GAAACAGAAT TGATACTCTC ATGTATCTAC TAGCATATCC ACAAAAACCC
ATGGTTAAGA CAAAAACCAT TGAATTGATA GAATTTGAGA AACTGCCAGC TGGACAGAAT
GCAACAGTTG CTGTGATGAG CTATAGTGGC TATGATATTG AAGATGCTCT TGTTTTAAAC
AAGGCCTCTT TAGACAGAGG CTTTGGGCGT TGCCTTGTAT ATAAAAATGC TAAATGTACG
TTGAAACGAT ACACCAATCA GACTTTTGAT AAAGTGATGG GGCCCATGTT GGATGCTGCT
ACAAGGAAAC CTATCTGGCG ACATGAAATC TTAGATGCAG ATGGTATTTG TTCTCCAGGT
GAGAAAGTAG AAAACAAACA AGTGCTTGTA AATAAGTCCA TGCCCACAGT GACTCAGATT
CCTTTGGAAG GAAGTAATGT ACCACAGCAA CCACAGTACA AAGATGTACC CATAACCTAC
AAAGGAGCAA CAGACTCATA TATTGAAAAA GTGATGATAT CTTCAAATGC TGAAGATGCT
TTTCTGATCA AAATGCTGCT GAGACAGACA AGGCGTCCAG AAATTGGAGA CAAATTCAGC
AGTCGTCATG GGCAAAAAGG TGTTTGTGGC TTGATCGTCC CCCAGGAAGA CATGCCATTT
TGTGATTCTG GCATCTGTCC GGACATCATC ATGAACCCAC ACGGCTTCCC ATCACGAATG
ACGGTGGGGA AGCTCATTGA GCTGCTGGCT GGCAAGGCCG GTGTGCTGGA CGGCAGATTC
CACTACGGCA CTGCGTTTGG AGGCAGTAAA GTGAAGGATG TGTGTGAGGA CCTCGTTCGC
CATGGTTATA ACTACTTGGG GAAAGACTAT GTTACATCCG GCATCACAGG TGAGCCCTTA
GAAGCATACA TCTATTTTGG CCCCGTGTAC TATCAGAAGC TGAAACACAT GGTGCTAGAT
AAAATGCATG CCCGGGCCCG GGGCCCACGA GCCGTCCTTA CCAGGCAACC CACTGAAGGA
CGGTCTCGTG ATGGTGGCTT GCGTCTCGGG GAAATGGAAC GTGACTGTTT AATCGGTTAT
GGAGCCAGTA TGCTTTTGCT AGAGAGACTA ATGATTTCAA GTGATGCCTT TGAGGTTGAT
GTCTGTGGGC AGTGTGGACT TCTGGGGTAT TCTGGCTGGT GCCATTACTG CAAGTCATCC
TGCCACGTGT CTTCCCTCCG TATTCCGTAT GCCTGCAAGC TGCTCTTCCA GGAACTACAG
TCTATGAACA TCATCCCCAG GTTAAAACTG TCCAAGTACA ATGAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001154180.1
CDS244..3471
Misc Feature(1)139..141
Misc Feature(2)244..3450
Misc Feature(3)292..3456
Misc Feature(4)order(382..384,2371..2373,2497..2499,2503..2505,2515..2523,2527..2529)
Misc Feature(5)order(1468..1470,1474..1482,1486..1488,1495..1497,1510..1512,2041..2043,2095..2103,2107..2109,2296..2307,2314..2316,2320..2328,2779..2781,2785..2787,2791..2793,2857..2859,2863..2865,2872..2874,2881..2883,2896..2898,2908..2910,2959..2961,3040..3042,3064..3072,3076..3078,3115..3117,3124..3132,3136..3141,3202..3210,3220..3222,3226..3231,3235..3237,3241..3258,3262..3279,3289..3291,3388..3390,3400..3402,3406..3408,3412..3417,3421..3423,3427..3438,3442..3444,3448..3450)
Misc Feature(6)1684..1869
Misc Feature(7)1930..2052
Misc Feature(8)order(2215..2220,2281..2283,2287..2289,2350..2352,2359..2361,2806..2808,2845..2847,2926..2931,2935..2937,3028..3030,3079..3081)
Misc Feature(9)order(2314..2316,2356..2358,2803..2805,3046..3048)
Misc Feature(10)order(2356..2361,2371..2373,2725..2727,2731..2733,2806..2814,2821..2823,2827..2832,3043..3066,3070..3072)
Exon (1)1..141
Exon (2)142..174
Exon (3)175..231
Exon (4)232..296
Exon (5)297..372
Exon (6)373..473
Exon (7)474..565
Exon (8)566..683
Exon (9)684..792
Exon (10)793..915
Exon (11)916..1035
Exon (12)1036..1170
Exon (13)1171..1332
Exon (14)1333..1533
Exon (15)1534..1696
Exon (16)1697..1850
Exon (17)1851..1925
Exon (18)1926..2024
Exon (19)2025..2152
Exon (20)2153..2362
Exon (21)2363..2521
Exon (22)2522..2639
Exon (23)2640..2782
Exon (24)2783..2886
Exon (25)2887..3053
Exon (26)3054..3167
Exon (27)3168..3341
Exon (28)3342..4120
Translation

Target ORF information:

RefSeq Version NM_001160708.1
Organism Homo sapiens(Human)
Definition Homo sapiens RNA polymerase III subunit B (POLR3B), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001160708.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGAAAGCCA ATGAAAAGGT TACAAGTGAC GCTGACCCTA TGTGGTACTT AAAATATCTT 
AATATCTATG TTGGGCTTCC TGATGTTGAA GAAAGCTTCA ATGTAACTAG ACCAGTGTCC
CCTCATGAGT GCCGTTTGAG AGACATGACA TACTCTGCCC CTATTACAGT GGATATTGAA
TATACCCGAG GCAGCCAGAG GATCATCCGC AATGCCTTAC CTATCGGCAG AATGCCCATA
ATGCTACGTA GTTCAAACTG TGTTCTTACA GGAAAAACGC CAGCAGAATT TGCCAAACTG
AACGAATGTC CCTTAGATCC AGGTGGCTAC TTCATTGTTA AAGGAGTAGA AAAAGTTATT
CTTATCCAAG AGCAGCTGTC TAAGAACAGG ATCATCGTGG AGGCTGATAG AAAAGGGGCT
GTTGGAGCTT CAGTTACCAG CTCTACCCAT GAGAAAAAAA GCAGAACCAA TATGGCTGTG
AAACAAGGAC GATTTTATTT GAGGCATAAT ACTTTGTCAG AAGATATACC CATTGTCATC
ATATTTAAGG CCATGGGTGT TGAGAGTGAC CAGGAAATTG TGCAGATGAT TGGAACAGAG
GAGCACGTGA TGGCTGCATT TGGGCCCAGT CTGGAAGAGT GCCAGAAAGC TCAGATTTTC
ACACAGATGC AGGCATTAAA ATATATAGGG AACAAAGTAA GAAGGCAAAG GATGTGGGGA
GGTGGACCAA AGAAAACCAA AATAGAAGAA GCAAGAGAGC TCCTGGCTTC CACCATTCTG
ACCCATGTCC CAGTTAAGGA ATTCAATTTC CGAGCCAAAT GTATCTATAC TGCAGTGATG
GTGCGAAGAG TTATTCTGGC CCAAGGAGAT AATAAAGTTG ACGACAGAGA TTATTATGGT
AACAAGCGAC TGGAATTGGC AGGACAGCTT TTATCTCTTC TTTTTGAAGA CTTGTTCAAA
AAATTTAATT CTGAAATGAA AAAGATTGCC GACCAGGTGA TTCCTAAGCA AAGAGCAGCC
CAGTTTGATG TTGTCAAACA CATGCGCCAA GACCAGATCA CCAATGGCAT GGTGAATGCT
ATTTCTACCG GAAATTGGTC TTTAAAGAGA TTTAAAATGG ACCGCCAGGG TGTAACCCAA
GTGCTGTCTC GCTTGTCATA TATATCCGCA CTGGGCATGA TGACAAGAAT CTCTTCCCAG
TTTGAAAAAA CGAGAAAAGT GAGTGGTCCT CGCTCCCTCC AGCCATCTCA GTGGGGAATG
CTGTGTCCTT CGGACACTCC TGAAGGAGAG GCATGTGGTT TGGTTAAAAA CTTGGCCCTT
ATGACACACA TCACAACTGA TATGGAAGAT GGACCCATTG TTAAATTAGC CAGTAACTTG
GGAGTAGAAG ATGTGAATTT ATTATGTGGG GAAGAGCTCT CTTACCCAAA TGTGTTTCTT
GTCTTTCTTA ATGGTAACAT CTTAGGTGTC ATTCGAGACC ACAAAAAGCT AGTGAATACA
TTTCGACTCA TGAGAAGAGC AGGATATATC AATGAATTTG TTTCCATCTC AACAAATCTT
ACAGATCGAT GTGTCTATAT TTCTTCTGAT GGGGGAAGGC TATGCAGACC CTACATAATT
GTCAAGAAAC AGAAGCCAGC AGTCACAAAT AAACATATGG AAGAGCTGGC CCAAGGGTAC
AGGAATTTTG AAGATTTCTT ACATGAGAGT CTGGTTGAAT ATTTAGATGT GAATGAAGAA
AATGATTGTA ACATTGCACT GTACGAACAC ACAATTAATA AAGACACCAC CCACTTGGAG
ATTGAACCCT TCACTCTTCT CGGCGTGTGT GCTGGACTTA TCCCATACCC TCACCATAAC
CAGTCACCGA GAAACACTTA TCAGTGTGCC ATGGGGAAAC AAGCCATGGG TACTATAGGA
TACAACCAGC GAAACAGAAT TGATACTCTC ATGTATCTAC TAGCATATCC ACAAAAACCC
ATGGTTAAGA CAAAAACCAT TGAATTGATA GAATTTGAGA AACTGCCAGC TGGACAGAAT
GCAACAGTTG CTGTGATGAG CTATAGTGGC TATGATATTG AAGATGCTCT TGTTTTAAAC
AAGGCCTCTT TAGACAGAGG CTTTGGGCGT TGCCTTGTAT ATAAAAATGC TAAATGTACG
TTGAAACGAT ACACCAATCA GACTTTTGAT AAAGTGATGG GGCCCATGTT GGATGCTGCT
ACAAGGAAAC CTATCTGGCG ACATGAAATC TTAGATGCAG ATGGTATTTG TTCTCCAGGT
GAGAAAGTAG AAAACAAACA AGTGCTTGTA AATAAGTCCA TGCCCACAGT GACTCAGATT
CCTTTGGAAG GAAGTAATGT ACCACAGCAA CCACAGTACA AAGATGTACC CATAACCTAC
AAAGGAGCAA CAGACTCATA TATTGAAAAA GTGATGATAT CTTCAAATGC TGAAGATGCT
TTTCTGATCA AAATGCTGCT GAGACAGACA AGGCGTCCAG AAATTGGAGA CAAATTCAGC
AGTCGTCATG GGCAAAAAGG TGTTTGTGGC TTGATCGTCC CCCAGGAAGA CATGCCATTT
TGTGATTCTG GCATCTGTCC GGACATCATC ATGAACCCAC ACGGCTTCCC ATCACGAATG
ACGGTGGGGA AGCTCATTGA GCTGCTGGCT GGCAAGGCCG GTGTGCTGGA CGGCAGATTC
CACTACGGCA CTGCGTTTGG AGGCAGTAAA GTGAAGGATG TGTGTGAGGA CCTCGTTCGC
CATGGTTATA ACTACTTGGG GAAAGACTAT GTTACATCCG GCATCACAGG TGAGCCCTTA
GAAGCATACA TCTATTTTGG CCCCGTGTAC TATCAGAAGC TGAAACACAT GGTGCTAGAT
AAAATGCATG CCCGGGCCCG GGGCCCACGA GCCGTCCTTA CCAGGCAACC CACTGAAGGA
CGGTCTCGTG ATGGTGGCTT GCGTCTCGGG GAAATGGAAC GTGACTGTTT AATCGGTTAT
GGAGCCAGTA TGCTTTTGCT AGAGAGACTA ATGATTTCAA GTGATGCCTT TGAGGTTGAT
GTCTGTGGGC AGTGTGGACT TCTGGGGTAT TCTGGCTGGT GCCATTACTG CAAGTCATCC
TGCCACGTGT CTTCCCTCCG TATTCCGTAT GCCTGCAAGC TGCTCTTCCA GGAACTACAG
TCTATGAACA TCATCCCCAG GTTAAAACTG TCCAAGTACA ATGAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27279
Clone ID Related Accession (Same CDS sequence) NM_018082.5
Accession Version NM_018082.5 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3402bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Homo sapiens(Human)
Product DNA-directed RNA polymerase III subunit RPC2 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA236776.1, BX537447.1 and BC033724.2. This sequence is a reference standard in the RefSeqGene project. On Jun 5, 2009 this sequence version replaced NM_018082.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX537447.1, SRR1803615.228111.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGGACGTGC TAGCGGAGGA GTTTGGGAAC CTGACTCCGG AGCAGCTGGC GGCGCCGATC 
CCGACTGTAG AGGAAAAATG GAGGCTGCTT CCAGCATTTT TAAAGGTGAA AGGCCTTGTG
AAACAGCATA TAGATTCATT TAACTATTTC ATTAATGTAG AGATAAAGAA GATAATGAAA
GCCAATGAAA AGGTTACAAG TGACGCTGAC CCTATGTGGT ACTTAAAATA TCTTAATATC
TATGTTGGGC TTCCTGATGT TGAAGAAAGC TTCAATGTAA CTAGACCAGT GTCCCCTCAT
GAGTGCCGTT TGAGAGACAT GACATACTCT GCCCCTATTA CAGTGGATAT TGAATATACC
CGAGGCAGCC AGAGGATCAT CCGCAATGCC TTACCTATCG GCAGAATGCC CATAATGCTA
CGTAGTTCAA ACTGTGTTCT TACAGGAAAA ACGCCAGCAG AATTTGCCAA ACTGAACGAA
TGTCCCTTAG ATCCAGGTGG CTACTTCATT GTTAAAGGAG TAGAAAAAGT TATTCTTATC
CAAGAGCAGC TGTCTAAGAA CAGGATCATC GTGGAGGCTG ATAGAAAAGG GGCTGTTGGA
GCTTCAGTTA CCAGCTCTAC CCATGAGAAA AAAAGCAGAA CCAATATGGC TGTGAAACAA
GGACGATTTT ATTTGAGGCA TAATACTTTG TCAGAAGATA TACCCATTGT CATCATATTT
AAGGCCATGG GTGTTGAGAG TGACCAGGAA ATTGTGCAGA TGATTGGAAC AGAGGAGCAC
GTGATGGCTG CATTTGGGCC CAGTCTGGAA GAGTGCCAGA AAGCTCAGAT TTTCACACAG
ATGCAGGCAT TAAAATATAT AGGGAACAAA GTAAGAAGGC AAAGGATGTG GGGAGGTGGA
CCAAAGAAAA CCAAAATAGA AGAAGCAAGA GAGCTCCTGG CTTCCACCAT TCTGACCCAT
GTCCCAGTTA AGGAATTCAA TTTCCGAGCC AAATGTATCT ATACTGCAGT GATGGTGCGA
AGAGTTATTC TGGCCCAAGG AGATAATAAA GTTGACGACA GAGATTATTA TGGTAACAAG
CGACTGGAAT TGGCAGGACA GCTTTTATCT CTTCTTTTTG AAGACTTGTT CAAAAAATTT
AATTCTGAAA TGAAAAAGAT TGCCGACCAG GTGATTCCTA AGCAAAGAGC AGCCCAGTTT
GATGTTGTCA AACACATGCG CCAAGACCAG ATCACCAATG GCATGGTGAA TGCTATTTCT
ACCGGAAATT GGTCTTTAAA GAGATTTAAA ATGGACCGCC AGGGTGTAAC CCAAGTGCTG
TCTCGCTTGT CATATATATC CGCACTGGGC ATGATGACAA GAATCTCTTC CCAGTTTGAA
AAAACGAGAA AAGTGAGTGG TCCTCGCTCC CTCCAGCCAT CTCAGTGGGG AATGCTGTGT
CCTTCGGACA CTCCTGAAGG AGAGGCATGT GGTTTGGTTA AAAACTTGGC CCTTATGACA
CACATCACAA CTGATATGGA AGATGGACCC ATTGTTAAAT TAGCCAGTAA CTTGGGAGTA
GAAGATGTGA ATTTATTATG TGGGGAAGAG CTCTCTTACC CAAATGTGTT TCTTGTCTTT
CTTAATGGTA ACATCTTAGG TGTCATTCGA GACCACAAAA AGCTAGTGAA TACATTTCGA
CTCATGAGAA GAGCAGGATA TATCAATGAA TTTGTTTCCA TCTCAACAAA TCTTACAGAT
CGATGTGTCT ATATTTCTTC TGATGGGGGA AGGCTATGCA GACCCTACAT AATTGTCAAG
AAACAGAAGC CAGCAGTCAC AAATAAACAT ATGGAAGAGC TGGCCCAAGG GTACAGGAAT
TTTGAAGATT TCTTACATGA GAGTCTGGTT GAATATTTAG ATGTGAATGA AGAAAATGAT
TGTAACATTG CACTGTACGA ACACACAATT AATAAAGACA CCACCCACTT GGAGATTGAA
CCCTTCACTC TTCTCGGCGT GTGTGCTGGA CTTATCCCAT ACCCTCACCA TAACCAGTCA
CCGAGAAACA CTTATCAGTG TGCCATGGGG AAACAAGCCA TGGGTACTAT AGGATACAAC
CAGCGAAACA GAATTGATAC TCTCATGTAT CTACTAGCAT ATCCACAAAA ACCCATGGTT
AAGACAAAAA CCATTGAATT GATAGAATTT GAGAAACTGC CAGCTGGACA GAATGCAACA
GTTGCTGTGA TGAGCTATAG TGGCTATGAT ATTGAAGATG CTCTTGTTTT AAACAAGGCC
TCTTTAGACA GAGGCTTTGG GCGTTGCCTT GTATATAAAA ATGCTAAATG TACGTTGAAA
CGATACACCA ATCAGACTTT TGATAAAGTG ATGGGGCCCA TGTTGGATGC TGCTACAAGG
AAACCTATCT GGCGACATGA AATCTTAGAT GCAGATGGTA TTTGTTCTCC AGGTGAGAAA
GTAGAAAACA AACAAGTGCT TGTAAATAAG TCCATGCCCA CAGTGACTCA GATTCCTTTG
GAAGGAAGTA ATGTACCACA GCAACCACAG TACAAAGATG TACCCATAAC CTACAAAGGA
GCAACAGACT CATATATTGA AAAAGTGATG ATATCTTCAA ATGCTGAAGA TGCTTTTCTG
ATCAAAATGC TGCTGAGACA GACAAGGCGT CCAGAAATTG GAGACAAATT CAGCAGTCGT
CATGGGCAAA AAGGTGTTTG TGGCTTGATC GTCCCCCAGG AAGACATGCC ATTTTGTGAT
TCTGGCATCT GTCCGGACAT CATCATGAAC CCACACGGCT TCCCATCACG AATGACGGTG
GGGAAGCTCA TTGAGCTGCT GGCTGGCAAG GCCGGTGTGC TGGACGGCAG ATTCCACTAC
GGCACTGCGT TTGGAGGCAG TAAAGTGAAG GATGTGTGTG AGGACCTCGT TCGCCATGGT
TATAACTACT TGGGGAAAGA CTATGTTACA TCCGGCATCA CAGGTGAGCC CTTAGAAGCA
TACATCTATT TTGGCCCCGT GTACTATCAG AAGCTGAAAC ACATGGTGCT AGATAAAATG
CATGCCCGGG CCCGGGGCCC ACGAGCCGTC CTTACCAGGC AACCCACTGA AGGACGGTCT
CGTGATGGTG GCTTGCGTCT CGGGGAAATG GAACGTGACT GTTTAATCGG TTATGGAGCC
AGTATGCTTT TGCTAGAGAG ACTAATGATT TCAAGTGATG CCTTTGAGGT TGATGTCTGT
GGGCAGTGTG GACTTCTGGG GTATTCTGGC TGGTGCCATT ACTGCAAGTC ATCCTGCCAC
GTGTCTTCCC TCCGTATTCC GTATGCCTGC AAGCTGCTCT TCCAGGAACT ACAGTCTATG
AACATCATCC CCAGGTTAAA ACTGTCCAAG TACAATGAAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_060552.4
CDS223..3624
Misc Feature(1)100..102
Misc Feature(2)298..3609
Misc Feature(3)337..3603
Misc Feature(4)order(535..537,2524..2526,2650..2652,2656..2658,2668..2676,2680..2682)
Misc Feature(5)order(1621..1623,1627..1635,1639..1641,1648..1650,1663..1665,2194..2196,2248..2256,2260..2262,2449..2460,2467..2469,2473..2481,2932..2934,2938..2940,2944..2946,3010..3012,3016..3018,3025..3027,3034..3036,3049..3051,3061..3063,3112..3114,3193..3195,3217..3225,3229..3231,3268..3270,3277..3285,3289..3294,3355..3363,3373..3375,3379..3384,3388..3390,3394..3411,3415..3432,3442..3444,3541..3543,3553..3555,3559..3561,3565..3570,3574..3576,3580..3591,3595..3597,3601..3603)
Misc Feature(6)1837..2022
Misc Feature(7)2083..2205
Misc Feature(8)order(2368..2373,2434..2436,2440..2442,2503..2505,2512..2514,2959..2961,2998..3000,3079..3084,3088..3090,3181..3183,3232..3234)
Misc Feature(9)order(2467..2469,2509..2511,2956..2958,3199..3201)
Misc Feature(10)order(2509..2514,2524..2526,2878..2880,2884..2886,2959..2967,2974..2976,2980..2985,3196..3219,3223..3225)
Exon (1)1..294
Exon (2)295..327
Exon (3)328..384
Exon (4)385..449
Exon (5)450..525
Exon (6)526..626
Exon (7)627..718
Exon (8)719..836
Exon (9)837..945
Exon (10)946..1068
Exon (11)1069..1188
Exon (12)1189..1323
Exon (13)1324..1485
Exon (14)1486..1686
Exon (15)1687..1849
Exon (16)1850..2003
Exon (17)2004..2078
Exon (18)2079..2177
Exon (19)2178..2305
Exon (20)2306..2515
Exon (21)2516..2674
Exon (22)2675..2792
Exon (23)2793..2935
Exon (24)2936..3039
Exon (25)3040..3206
Exon (26)3207..3320
Exon (27)3321..3494
Exon (28)3495..4273
Translation

Target ORF information:

RefSeq Version NM_018082.5
Organism Homo sapiens(Human)
Definition Homo sapiens RNA polymerase III subunit B (POLR3B), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_018082.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGGACGTGC TAGCGGAGGA GTTTGGGAAC CTGACTCCGG AGCAGCTGGC GGCGCCGATC 
CCGACTGTAG AGGAAAAATG GAGGCTGCTT CCAGCATTTT TAAAGGTGAA AGGCCTTGTG
AAACAGCATA TAGATTCATT TAACTATTTC ATTAATGTAG AGATAAAGAA GATAATGAAA
GCCAATGAAA AGGTTACAAG TGACGCTGAC CCTATGTGGT ACTTAAAATA TCTTAATATC
TATGTTGGGC TTCCTGATGT TGAAGAAAGC TTCAATGTAA CTAGACCAGT GTCCCCTCAT
GAGTGCCGTT TGAGAGACAT GACATACTCT GCCCCTATTA CAGTGGATAT TGAATATACC
CGAGGCAGCC AGAGGATCAT CCGCAATGCC TTACCTATCG GCAGAATGCC CATAATGCTA
CGTAGTTCAA ACTGTGTTCT TACAGGAAAA ACGCCAGCAG AATTTGCCAA ACTGAACGAA
TGTCCCTTAG ATCCAGGTGG CTACTTCATT GTTAAAGGAG TAGAAAAAGT TATTCTTATC
CAAGAGCAGC TGTCTAAGAA CAGGATCATC GTGGAGGCTG ATAGAAAAGG GGCTGTTGGA
GCTTCAGTTA CCAGCTCTAC CCATGAGAAA AAAAGCAGAA CCAATATGGC TGTGAAACAA
GGACGATTTT ATTTGAGGCA TAATACTTTG TCAGAAGATA TACCCATTGT CATCATATTT
AAGGCCATGG GTGTTGAGAG TGACCAGGAA ATTGTGCAGA TGATTGGAAC AGAGGAGCAC
GTGATGGCTG CATTTGGGCC CAGTCTGGAA GAGTGCCAGA AAGCTCAGAT TTTCACACAG
ATGCAGGCAT TAAAATATAT AGGGAACAAA GTAAGAAGGC AAAGGATGTG GGGAGGTGGA
CCAAAGAAAA CCAAAATAGA AGAAGCAAGA GAGCTCCTGG CTTCCACCAT TCTGACCCAT
GTCCCAGTTA AGGAATTCAA TTTCCGAGCC AAATGTATCT ATACTGCAGT GATGGTGCGA
AGAGTTATTC TGGCCCAAGG AGATAATAAA GTTGACGACA GAGATTATTA TGGTAACAAG
CGACTGGAAT TGGCAGGACA GCTTTTATCT CTTCTTTTTG AAGACTTGTT CAAAAAATTT
AATTCTGAAA TGAAAAAGAT TGCCGACCAG GTGATTCCTA AGCAAAGAGC AGCCCAGTTT
GATGTTGTCA AACACATGCG CCAAGACCAG ATCACCAATG GCATGGTGAA TGCTATTTCT
ACCGGAAATT GGTCTTTAAA GAGATTTAAA ATGGACCGCC AGGGTGTAAC CCAAGTGCTG
TCTCGCTTGT CATATATATC CGCACTGGGC ATGATGACAA GAATCTCTTC CCAGTTTGAA
AAAACGAGAA AAGTGAGTGG TCCTCGCTCC CTCCAGCCAT CTCAGTGGGG AATGCTGTGT
CCTTCGGACA CTCCTGAAGG AGAGGCATGT GGTTTGGTTA AAAACTTGGC CCTTATGACA
CACATCACAA CTGATATGGA AGATGGACCC ATTGTTAAAT TAGCCAGTAA CTTGGGAGTA
GAAGATGTGA ATTTATTATG TGGGGAAGAG CTCTCTTACC CAAATGTGTT TCTTGTCTTT
CTTAATGGTA ACATCTTAGG TGTCATTCGA GACCACAAAA AGCTAGTGAA TACATTTCGA
CTCATGAGAA GAGCAGGATA TATCAATGAA TTTGTTTCCA TCTCAACAAA TCTTACAGAT
CGATGTGTCT ATATTTCTTC TGATGGGGGA AGGCTATGCA GACCCTACAT AATTGTCAAG
AAACAGAAGC CAGCAGTCAC AAATAAACAT ATGGAAGAGC TGGCCCAAGG GTACAGGAAT
TTTGAAGATT TCTTACATGA GAGTCTGGTT GAATATTTAG ATGTGAATGA AGAAAATGAT
TGTAACATTG CACTGTACGA ACACACAATT AATAAAGACA CCACCCACTT GGAGATTGAA
CCCTTCACTC TTCTCGGCGT GTGTGCTGGA CTTATCCCAT ACCCTCACCA TAACCAGTCA
CCGAGAAACA CTTATCAGTG TGCCATGGGG AAACAAGCCA TGGGTACTAT AGGATACAAC
CAGCGAAACA GAATTGATAC TCTCATGTAT CTACTAGCAT ATCCACAAAA ACCCATGGTT
AAGACAAAAA CCATTGAATT GATAGAATTT GAGAAACTGC CAGCTGGACA GAATGCAACA
GTTGCTGTGA TGAGCTATAG TGGCTATGAT ATTGAAGATG CTCTTGTTTT AAACAAGGCC
TCTTTAGACA GAGGCTTTGG GCGTTGCCTT GTATATAAAA ATGCTAAATG TACGTTGAAA
CGATACACCA ATCAGACTTT TGATAAAGTG ATGGGGCCCA TGTTGGATGC TGCTACAAGG
AAACCTATCT GGCGACATGA AATCTTAGAT GCAGATGGTA TTTGTTCTCC AGGTGAGAAA
GTAGAAAACA AACAAGTGCT TGTAAATAAG TCCATGCCCA CAGTGACTCA GATTCCTTTG
GAAGGAAGTA ATGTACCACA GCAACCACAG TACAAAGATG TACCCATAAC CTACAAAGGA
GCAACAGACT CATATATTGA AAAAGTGATG ATATCTTCAA ATGCTGAAGA TGCTTTTCTG
ATCAAAATGC TGCTGAGACA GACAAGGCGT CCAGAAATTG GAGACAAATT CAGCAGTCGT
CATGGGCAAA AAGGTGTTTG TGGCTTGATC GTCCCCCAGG AAGACATGCC ATTTTGTGAT
TCTGGCATCT GTCCGGACAT CATCATGAAC CCACACGGCT TCCCATCACG AATGACGGTG
GGGAAGCTCA TTGAGCTGCT GGCTGGCAAG GCCGGTGTGC TGGACGGCAG ATTCCACTAC
GGCACTGCGT TTGGAGGCAG TAAAGTGAAG GATGTGTGTG AGGACCTCGT TCGCCATGGT
TATAACTACT TGGGGAAAGA CTATGTTACA TCCGGCATCA CAGGTGAGCC CTTAGAAGCA
TACATCTATT TTGGCCCCGT GTACTATCAG AAGCTGAAAC ACATGGTGCT AGATAAAATG
CATGCCCGGG CCCGGGGCCC ACGAGCCGTC CTTACCAGGC AACCCACTGA AGGACGGTCT
CGTGATGGTG GCTTGCGTCT CGGGGAAATG GAACGTGACT GTTTAATCGG TTATGGAGCC
AGTATGCTTT TGCTAGAGAG ACTAATGATT TCAAGTGATG CCTTTGAGGT TGATGTCTGT
GGGCAGTGTG GACTTCTGGG GTATTCTGGC TGGTGCCATT ACTGCAAGTC ATCCTGCCAC
GTGTCTTCCC TCCGTATTCC GTATGCCTGC AAGCTGCTCT TCCAGGAACT ACAGTCTATG
AACATCATCC CCAGGTTAAA ACTGTCCAAG TACAATGAAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu83968
Clone ID Related Accession (Same CDS sequence) XM_017019621.1
Accession Version XM_017019621.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3075bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product DNA-directed RNA polymerase III subunit RPC2 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
ATGGACGTGC TAGCGGAGGA GTTTGGGAAC CTGACTCCGG AGCAGCTGGC GGCGCCGATC 
CCGACTGTAG AGGAAAAATG GAGGCTGCTT CCAGCATTTT TAAAGGTGAA AGGCCTTGTG
AAACAGCATA TAGATTCATT TAACTATTTC ATTAATGTAG AGATAAAGAA GATAATGAAA
GCCAATGAAA AGGTTACAAG TGACGCTGAC CCTATGTGGT ACTTAAAATA TCTTAATATC
TATGTTGGGC TTCCTGATGT TGAAGAAAGC TTCAATGTAA CTAGACCAGT GTCCCCTCAT
GAGTGCCGTT TGAGAGACAT GACATACTCT GCCCCTATTA CAGTGGATAT TGAATATACC
CGAGGCAGCC AGAGGATCAT CCGCAATGCC TTACCTATCG GCAGAATGCC CATAATGCTA
CGTAGTTCAA ACTGTGTTCT TACAGGAAAA ACGCCAGCAG AATTTGCCAA ACTGAACGAA
TGTCCCTTAG ATCCAGGTGG CTACTTCATT GTTAAAGGAG TAGAAAAAGT TATTCTTATC
CAAGAGCAGC TGTCTAAGAA CAGGATCATC GTGGAGGCTG ATAGAAAAGG GGCTGTTGGA
GCTTCAGTTA CCAGCTCTAC CCATGAGAAA AAAAGCAGAA CCAATATGGC TGTGAAACAA
GGACGATTTT ATTTGAGGCA TAATACTTTG TCAGAAGATA TACCCATTGT CATCATATTT
AAGGCCATGG GTGTTGAGAG TGACCAGGAA ATTGTGCAGA TGATTGGAAC AGAGGAGCAC
GTGATGGCTG CATTTGGGCC CAGTCTGGAA GAGTGCCAGA AAGCTCAGAT TTTCACACAG
ATGCAGGCAT TAAAATATAT AGGGAACAAA GTAAGAAGGC AAAGGATGTG GGGAGGTGGA
CCAAAGAAAA CCAAAATAGA AGAAGCAAGA GAGCTCCTGG CTTCCACCAT TCTGACCCAT
GTCCCAGTTA AGGAATTCAA TTTCCGAGCC AAATGTATCT ATACTGCAGT GATGGTGCGA
AGAGTTATTC TGGCCCAAGG AGATAATAAA GTTGACGACA GAGATTATTA TGGTAACAAG
CGACTGGAAT TGGCAGGACA GCTTTTATCT CTTCTTTTTG AAGACTTGTT CAAAAAATTT
AATTCTGAAA TGAAAAAGAT TGCCGACCAG GTGATTCCTA AGCAAAGAGC AGCCCAGTTT
GATGTTGTCA AACACATGCG CCAAGACCAG ATCACCAATG GCATGGTGAA TGCTATTTCT
ACCGGAAATT GGTCTTTAAA GAGATTTAAA ATGGACCGCC AGGGTGTAAC CCAAGTGCTG
TCTCGCTTGT CATATATATC CGCACTGGGC ATGATGACAA GAATCTCTTC CCAGTTTGAA
AAAACGAGAA AAGTGAGTGG TCCTCGCTCC CTCCAGCCAT CTCAGTGGGG AATGCTGTGT
CCTTCGGACA CTCCTGAAGG AGAGGCATGT GGTTTGGTTA AAAACTTGGC CCTTATGACA
CACATCACAA CTGATATGGA AGATGGACCC ATTGTTAAAT TAGCCAGTAA CTTGGGAGTA
GAAGATGTGA ATTTATTATG TGGGGAAGAG CTCTCTTACC CAAATGTGTT TCTTGTCTTT
CTTAATGGTA ACATCTTAGG TGTCATTCGA GACCACAAAA AGCTAGTGAA TACATTTCGA
CTCATGAGAA GAGCAGGATA TATCAATGAA TTTGTTTCCA TCTCAACAAA TCTTACAGAT
CGATGTGTCT ATATTTCTTC TGATGGGGGA AGGCTATGCA GACCCTACAT AATTGTCAAG
AAACAGAAGC CAGCAGTCAC AAATAAACAT ATGGAAGAGC TGGCCCAAGG GTACAGGAAT
TTTGAAGATT TCTTACATGA GAGTCTGGTT GAATATTTAG ATGTGAATGA AGAAAATGAT
TGTAACATTG CACTGTACGA ACACACAATT AATAAAGACA CCACCCACTT GGAGATTGAA
CCCTTCACTC TTCTCGGCGT GTGTGCTGGA CTTATCCCAT ACCCTCACCA TAACCAGTCA
CCGAGAAACA CTTATCAGTG TGCCATGGGG AAACAAGCCA TGGGTACTAT AGGATACAAC
CAGCGAAACA GAATTGATAC TCTCATGTAT CTACTAGCAT ATCCACAAAA ACCCATGGTT
AAGACAAAAA CCATTGAATT GATAGAATTT GAGAAACTGC CAGCTGGACA GAATGCAACA
GTTGCTGTGA TGAGCTATAG TGGCTATGAT ATTGAAGATG CTCTTGTTTT AAACAAGGCC
TCTTTAGACA GAGGCTTTGG GCGTTGCCTT GTATATAAAA ATGCTAAATG TACGTTGAAA
CGATACACCA ATCAGACTTT TGATAAAGTG ATGGGGCCCA TGTTGGATGC TGCTACAAGG
AAACCTATCT GGCGACATGA AATCTTAGAT GCAGATGGTA TTTGTTCTCC AGGTGAGAAA
GTAGAAAACA AACAAGTGCT TGTAAATAAG TCCATGCCCA CAGTGACTCA GATTCCTTTG
GAAGGAAGTA ATGTACCACA GCAACCACAG TACAAAGATG TACCCATAAC CTACAAAGGA
GCAACAGACT CATATATTGA AAAAGTGATG ATATCTTCAA ATGCTGAAGA TGCTTTTCTG
ATCAAAATGC TGCTGAGACA GACAAGGCGT CCAGAAATTG GAGACAAATT CAGCAGTCGT
CATGGGCAAA AAGGTGTTTG TGGCTTGATC GTCCCCCAGG AAGACATGCC ATTTTGTGAT
TCTGGCATCT GTCCGGACAT CATCATGAAC CCACACGGCT TCCCATCACG AATGACGGTG
GGGAAGCTCA TTGAGCTGCT GGCTGGCAAG GCCGGTGTGC TGGACGGCAG ATTCCACTAC
GGCACTGCGT TTGGAGGCAG TAAAGTGAAG GATGTGTGTG AGGACCTCGT TCGCCATGGT
TATAACTACT TGGGGAAAGA CTATGTTACA TCCGGCATCA CAGGAAATAA GGCACATTTT
TGGCCAGGTG TTGACCAGAA AGTGCAAAAC CATAGGATCA ATTTGGTGGA CCTTCGTGAA
CATAATTTGT ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016875110.1
CDS188..3262
Translation

Target ORF information:

RefSeq Version XM_017019621.1
Organism Homo sapiens(Human)
Definition Homo sapiens polymerase (RNA) III subunit B (POLR3B), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017019621.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
ATGGACGTGC TAGCGGAGGA GTTTGGGAAC CTGACTCCGG AGCAGCTGGC GGCGCCGATC 
CCGACTGTAG AGGAAAAATG GAGGCTGCTT CCAGCATTTT TAAAGGTGAA AGGCCTTGTG
AAACAGCATA TAGATTCATT TAACTATTTC ATTAATGTAG AGATAAAGAA GATAATGAAA
GCCAATGAAA AGGTTACAAG TGACGCTGAC CCTATGTGGT ACTTAAAATA TCTTAATATC
TATGTTGGGC TTCCTGATGT TGAAGAAAGC TTCAATGTAA CTAGACCAGT GTCCCCTCAT
GAGTGCCGTT TGAGAGACAT GACATACTCT GCCCCTATTA CAGTGGATAT TGAATATACC
CGAGGCAGCC AGAGGATCAT CCGCAATGCC TTACCTATCG GCAGAATGCC CATAATGCTA
CGTAGTTCAA ACTGTGTTCT TACAGGAAAA ACGCCAGCAG AATTTGCCAA ACTGAACGAA
TGTCCCTTAG ATCCAGGTGG CTACTTCATT GTTAAAGGAG TAGAAAAAGT TATTCTTATC
CAAGAGCAGC TGTCTAAGAA CAGGATCATC GTGGAGGCTG ATAGAAAAGG GGCTGTTGGA
GCTTCAGTTA CCAGCTCTAC CCATGAGAAA AAAAGCAGAA CCAATATGGC TGTGAAACAA
GGACGATTTT ATTTGAGGCA TAATACTTTG TCAGAAGATA TACCCATTGT CATCATATTT
AAGGCCATGG GTGTTGAGAG TGACCAGGAA ATTGTGCAGA TGATTGGAAC AGAGGAGCAC
GTGATGGCTG CATTTGGGCC CAGTCTGGAA GAGTGCCAGA AAGCTCAGAT TTTCACACAG
ATGCAGGCAT TAAAATATAT AGGGAACAAA GTAAGAAGGC AAAGGATGTG GGGAGGTGGA
CCAAAGAAAA CCAAAATAGA AGAAGCAAGA GAGCTCCTGG CTTCCACCAT TCTGACCCAT
GTCCCAGTTA AGGAATTCAA TTTCCGAGCC AAATGTATCT ATACTGCAGT GATGGTGCGA
AGAGTTATTC TGGCCCAAGG AGATAATAAA GTTGACGACA GAGATTATTA TGGTAACAAG
CGACTGGAAT TGGCAGGACA GCTTTTATCT CTTCTTTTTG AAGACTTGTT CAAAAAATTT
AATTCTGAAA TGAAAAAGAT TGCCGACCAG GTGATTCCTA AGCAAAGAGC AGCCCAGTTT
GATGTTGTCA AACACATGCG CCAAGACCAG ATCACCAATG GCATGGTGAA TGCTATTTCT
ACCGGAAATT GGTCTTTAAA GAGATTTAAA ATGGACCGCC AGGGTGTAAC CCAAGTGCTG
TCTCGCTTGT CATATATATC CGCACTGGGC ATGATGACAA GAATCTCTTC CCAGTTTGAA
AAAACGAGAA AAGTGAGTGG TCCTCGCTCC CTCCAGCCAT CTCAGTGGGG AATGCTGTGT
CCTTCGGACA CTCCTGAAGG AGAGGCATGT GGTTTGGTTA AAAACTTGGC CCTTATGACA
CACATCACAA CTGATATGGA AGATGGACCC ATTGTTAAAT TAGCCAGTAA CTTGGGAGTA
GAAGATGTGA ATTTATTATG TGGGGAAGAG CTCTCTTACC CAAATGTGTT TCTTGTCTTT
CTTAATGGTA ACATCTTAGG TGTCATTCGA GACCACAAAA AGCTAGTGAA TACATTTCGA
CTCATGAGAA GAGCAGGATA TATCAATGAA TTTGTTTCCA TCTCAACAAA TCTTACAGAT
CGATGTGTCT ATATTTCTTC TGATGGGGGA AGGCTATGCA GACCCTACAT AATTGTCAAG
AAACAGAAGC CAGCAGTCAC AAATAAACAT ATGGAAGAGC TGGCCCAAGG GTACAGGAAT
TTTGAAGATT TCTTACATGA GAGTCTGGTT GAATATTTAG ATGTGAATGA AGAAAATGAT
TGTAACATTG CACTGTACGA ACACACAATT AATAAAGACA CCACCCACTT GGAGATTGAA
CCCTTCACTC TTCTCGGCGT GTGTGCTGGA CTTATCCCAT ACCCTCACCA TAACCAGTCA
CCGAGAAACA CTTATCAGTG TGCCATGGGG AAACAAGCCA TGGGTACTAT AGGATACAAC
CAGCGAAACA GAATTGATAC TCTCATGTAT CTACTAGCAT ATCCACAAAA ACCCATGGTT
AAGACAAAAA CCATTGAATT GATAGAATTT GAGAAACTGC CAGCTGGACA GAATGCAACA
GTTGCTGTGA TGAGCTATAG TGGCTATGAT ATTGAAGATG CTCTTGTTTT AAACAAGGCC
TCTTTAGACA GAGGCTTTGG GCGTTGCCTT GTATATAAAA ATGCTAAATG TACGTTGAAA
CGATACACCA ATCAGACTTT TGATAAAGTG ATGGGGCCCA TGTTGGATGC TGCTACAAGG
AAACCTATCT GGCGACATGA AATCTTAGAT GCAGATGGTA TTTGTTCTCC AGGTGAGAAA
GTAGAAAACA AACAAGTGCT TGTAAATAAG TCCATGCCCA CAGTGACTCA GATTCCTTTG
GAAGGAAGTA ATGTACCACA GCAACCACAG TACAAAGATG TACCCATAAC CTACAAAGGA
GCAACAGACT CATATATTGA AAAAGTGATG ATATCTTCAA ATGCTGAAGA TGCTTTTCTG
ATCAAAATGC TGCTGAGACA GACAAGGCGT CCAGAAATTG GAGACAAATT CAGCAGTCGT
CATGGGCAAA AAGGTGTTTG TGGCTTGATC GTCCCCCAGG AAGACATGCC ATTTTGTGAT
TCTGGCATCT GTCCGGACAT CATCATGAAC CCACACGGCT TCCCATCACG AATGACGGTG
GGGAAGCTCA TTGAGCTGCT GGCTGGCAAG GCCGGTGTGC TGGACGGCAG ATTCCACTAC
GGCACTGCGT TTGGAGGCAG TAAAGTGAAG GATGTGTGTG AGGACCTCGT TCGCCATGGT
TATAACTACT TGGGGAAAGA CTATGTTACA TCCGGCATCA CAGGAAATAA GGCACATTTT
TGGCCAGGTG TTGACCAGAA AGTGCAAAAC CATAGGATCA ATTTGGTGGA CCTTCGTGAA
CATAATTTGT ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Diffuse hypomyelination is not obligate for POLR3-related disorders.
Neurology86(17)1622-6(2016 Apr)
La Piana R,Cayami FK,Tran LT,Guerrero K,van Spaendonk R,Õunap K,Pajusalu S,Haack T,Wassmer E,Timmann D,Mierzewska H,Poll-Thé BT,Patel C,Cox H,Atik T,Onay H,Ozkınay F,Vanderver A,van der Knaap MS,Wolf NI,Bernard G


book

Large exonic deletions in POLR3B gene cause POLR3-related leukodystrophy.
Orphanet journal of rare diseases1069(2015 Jun)
Gutierrez M,Thiffault I,Guerrero K,Martos-Moreno GÁ,Tran LT,Benko W,van der Knaap MS,van Spaendonk RM,Wolf NI,Bernard G


book

Recessive mutations in POLR3B, encoding the second largest subunit of Pol III, cause a rare hypomyelinating leukodystrophy.
American journal of human genetics89(5)652-5(2011 Nov)
Tétreault M,Choquet K,Orcesi S,Tonduti D,Balottin U,Teichmann M,Fribourg S,Schiffmann R,Brais B,Vanderver A,Bernard G


 
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