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VPS35 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol VPS35
Entrez Gene ID 55737
Full Name VPS35, retromer complex component
Synonyms MEM3,PARK17
General protein information
Preferred Names

VPS35, retromer complex component

Names

vacuolar protein sorting-associated protein 35
hVPS35
maternal-embryonic 3
vacuolar protein sorting 35 homolog

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

16

16q11.2

Summary This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq, Jul 2008].
Disorder MIM:

601501


mRNA and Protein(s)

mRNA Protein Name
NM_018206.5 NP_060676.2 vacuolar protein sorting-associated protein 35
XM_005256045.3 XP_005256102.1 vacuolar protein sorting-associated protein 35 isoform X2
XM_011523227.2 XP_011521529.1 vacuolar protein sorting-associated protein 35 isoform X1
NM_018206.4 NP_060676.2 vacuolar protein sorting-associated protein 35



Homo sapiens (human) VPS35 NP_060676.2
Canis lupus familiaris (dog) VPS35 XP_532570.3
Eremothecium gossypii AGOS_AEL107W NP_984754.1
Arabidopsis thaliana (thale cress) VPS35B NP_177713.3
Rattus norvegicus (Norway rat) Vps35 NP_001099188.2
Danio rerio (zebrafish) vps35 NP_001020688.2
Macaca mulatta (rhesus monkey) VPS35 XP_001108828.2
Gallus gallus (chicken) VPS35 NP_001005842.1
Caenorhabditis elegans (roundworm) vps-35 NP_495180.2
Saccharomyces cerevisiae (baker's yeast) VPS35 NP_012381.1
Kluyveromyces lactis KLLA0B07535g XP_451864.1
Magnaporthe oryzae (rice blast fungus) MGG_05089 XP_003712611.1
Bos taurus (cattle) VPS35 NP_001039723.1
Drosophila melanogaster (fruit fly) Vps35 NP_611651.4
Anopheles gambiae (African malaria mosquito) AgaP_AGAP007683 XP_308188.4
Schizosaccharomyces pombe (fission yeast) vps35 NP_588260.2
Oryza sativa (rice) Os03g0801600 NP_001051593.1
Pan troglodytes (chimpanzee) VPS35 XP_001161536.1
Mus musculus (house mouse) Vps35 NP_075373.1
Neurospora crassa NCU06284 XP_962546.1


Related articles in PubMed

VPS35 regulates cell surface recycling and signaling of dopamine receptor D1.
Wang C, Niu M, Zhou Z, Zheng X, Zhang L, Tian Y, Yu X, Bu G, Xu H, Ma Q, Zhang YW
Neurobiology of aging4622-31(2016 Oct)

Parkinson Disease-linked Vps35 R524W Mutation Impairs the Endosomal Association of Retromer and Induces α-Synuclein Aggregation.
Follett J, Bugarcic A, Yang Z, Ariotti N, Norwood SJ, Collins BM, Parton RG, Teasdale RD
The Journal of biological chemistry291(35)18283-98(2016 Aug)

Parkinson's disease-associated mutant VPS35 causes mitochondrial dysfunction by recycling DLP1 complexes.
Wang W, Wang X, Fujioka H, Hoppel C, Whone AL, Caldwell MA, Cullen PJ, Liu J, Zhu X
Nature medicine22(1)54-63(2016 Jan)

An assessment of the frequency of mutations in the GBA and VPS35 genes in Hungarian patients with sporadic Parkinson's disease.
Török R, Zádori D, Török N, Csility É, Vécsei L, Klivényi P
Neuroscience letters610135-8(2016 Jan)

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA, Weisswange I, Mansfeld J, Buchholz F, Hyman AA, Mann M
Cell163(3)712-23(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results lend further support to the notion that VPS35-DLP1 interaction is key to the retromer-dependent recycling of mitochondrial DLP1 complex during mitochondrial fission and provide a novel therapeutic target to control excessive fission and associated mitochondrial deficits.
Title: A conserved retromer sorting motif is essential for mitochondrial DLP1 recycling by VPS35 in Parkinson's disease model.

X-ray crystallographic analysis of a 4-component complex comprising the VPS26 & VPS35 subunits of retromer, sorting nexin SNX3, & recycling signal from the divalent cation transporter DMT1-II; analysis identifies a binding site for canonical recycling signals at the interface between VPS26 & SNX3; shows cooperative interactions among the VPS subunits, SNX3 & cargo that couple signal-recognition to membrane recruitment.
Title: Structural Mechanism for Cargo Recognition by the Retromer Complex.

The absence of mutations in VPS35 genes reveals that they are uncommon causes of Parkinson disease in Brazil
Title: Autosomal dominant Parkinson's disease: Incidence of mutations in LRRK2, SNCA, VPS35 and GBA genes in Brazil.

Silencing VPS35 increased N-Ras's association with cytoplasmic vesicles, diminished GTP loading of Ras, and inhibited mitogen-activated protein kinase signaling and growth of N-Ras-dependent melanoma cells.
Title: VPS35 binds farnesylated N-Ras in the cytosol to regulate N-Ras trafficking.

the Vps35 R524W-containing retromer has a decreased endosomal association, which can be partially rescued by R55, a small molecule previously shown to stabilize the retromer complex, supporting the potential for future targeting of the retromer complex in the treatment of Parkinson disease.
Title: Parkinson Disease-linked Vps35 R524W Mutation Impairs the Endosomal Association of Retromer and Induces α-Synuclein Aggregation.

The following VPS35 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS35 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu18510 NM_018206.5
Latest version!
Homo sapiens VPS35, retromer complex component (VPS35), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu49428 XM_005256045.3
Latest version!
Homo sapiens VPS35 retromer complex component (VPS35), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu69690 XM_011523227.2
Latest version!
Homo sapiens VPS35 retromer complex component (VPS35), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
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OHu18510 NM_018206.4 Homo sapiens VPS35 retromer complex component (VPS35), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18510
Clone ID Related Accession (Same CDS sequence) NM_018206.5 , NM_018206.4
Accession Version NM_018206.5 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2391bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product vacuolar protein sorting-associated protein 35
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB049686.1, BC093036.1, BQ574179.1, AC012186.8 and BU621624.1. This sequence is a reference standard in the RefSeqGene project. On Apr 15, 2016 this sequence version replaced NM_018206.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.1800.1, SRR1803613.283710.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGCCTACAA CACAGCAGTC CCCTCAGGAT GAGCAGGAAA AGCTCTTGGA TGAAGCCATA 
CAGGCTGTGA AGGTCCAGTC ATTCCAAATG AAGAGATGCC TGGACAAAAA CAAGCTTATG
GATGCTCTAA AACATGCTTC TAATATGCTT GGTGAACTCC GGACTTCTAT GTTATCACCA
AAGAGTTACT ATGAACTTTA TATGGCCATT TCTGATGAAC TGCACTACTT GGAGGTCTAC
CTGACAGATG AGTTTGCTAA AGGAAGGAAA GTGGCAGATC TCTACGAACT TGTACAGTAT
GCTGGAAACA TTATCCCAAG GCTTTACCTT TTGATCACAG TTGGAGTTGT ATATGTCAAG
TCATTTCCTC AGTCCAGGAA GGATATTTTG AAAGATTTGG TAGAAATGTG CCGTGGTGTG
CAACATCCCT TGAGGGGTCT GTTTCTTCGA AATTACCTTC TTCAGTGTAC CAGAAATATC
TTACCTGATG AAGGAGAGCC AACAGATGAA GAAACAACTG GTGACATCAG TGATTCCATG
GATTTTGTAC TGCTCAACTT TGCAGAAATG AACAAGCTCT GGGTGCGAAT GCAGCATCAG
GGACATAGCC GAGATAGAGA AAAAAGAGAA CGAGAAAGAC AAGAACTGAG AATTTTAGTG
GGAACAAATT TGGTGCGCCT CAGTCAGTTG GAAGGTGTAA ATGTGGAACG TTACAAACAG
ATTGTTTTGA CTGGCATATT GGAGCAAGTT GTAAACTGTA GGGATGCTTT GGCTCAAGAA
TATCTCATGG AGTGTATTAT TCAGGTTTTC CCTGATGAAT TTCACCTCCA GACTTTGAAT
CCTTTTCTTC GGGCCTGTGC TGAGTTACAC CAGAATGTAA ATGTGAAGAA CATAATCATT
GCTTTAATTG ATAGATTAGC TTTATTTGCT CACCGTGAAG ATGGACCTGG AATCCCAGCG
GATATTAAAC TTTTTGATAT ATTTTCACAG CAGGTGGCTA CAGTGATACA GTCTAGACAA
GACATGCCTT CAGAGGATGT TGTATCTTTA CAAGTCTCTC TGATTAATCT TGCCATGAAA
TGTTACCCTG ATCGTGTGGA CTATGTTGAT AAAGTTCTAG AAACAACAGT GGAGATATTC
AATAAGCTCA ACCTTGAACA TATTGCTACC AGTAGTGCAG TTTCAAAGGA ACTCACCAGA
CTTTTGAAAA TACCAGTTGA CACTTACAAC AATATTTTAA CAGTCTTGAA ATTAAAACAT
TTTCACCCAC TCTTTGAGTA CTTTGACTAC GAGTCCAGAA AGAGCATGAG TTGTTATGTG
CTTAGTAATG TTCTGGATTA TAACACAGAA ATTGTCTCTC AAGACCAGGT GGATTCCATA
ATGAATTTGG TATCCACGTT GATTCAAGAT CAGCCAGATC AACCTGTAGA AGACCCTGAT
CCAGAAGATT TTGCTGATGA GCAGAGCCTT GTGGGCCGCT TCATTCATCT GCTGCGCTCT
GAGGACCCTG ACCAGCAGTA CTTGATTTTG AACACAGCAC GAAAACATTT TGGAGCTGGT
GGAAATCAGC GGATTCGCTT CACACTGCCA CCTTTGGTAT TTGCAGCTTA CCAGCTGGCT
TTTCGATATA AAGAGAATTC TAAAGTGGAT GACAAATGGG AAAAGAAATG CCAGAAGATT
TTTTCATTTG CCCACCAGAC TATCAGTGCT TTGATCAAAG CAGAGCTGGC AGAATTGCCC
TTAAGACTTT TTCTTCAAGG AGCACTAGCT GCTGGGGAAA TTGGTTTTGA AAATCATGAG
ACAGTCGCAT ATGAATTCAT GTCCCAGGCA TTTTCTCTGT ATGAAGATGA AATCAGCGAT
TCCAAAGCAC AGCTAGCTGC CATCACCTTG ATCATTGGCA CTTTTGAAAG GATGAAGTGC
TTCAGTGAAG AGAATCATGA ACCTCTGAGG ACTCAGTGTG CCCTTGCTGC ATCCAAACTT
CTAAAGAAAC CTGATCAGGG CCGAGCTGTG AGCACCTGTG CACATCTCTT CTGGTCTGGC
AGAAACACGG ACAAAAATGG GGAGGAGCTT CACGGAGGCA AGAGGGTAAT GGAGTGCCTA
AAAAAAGCTC TAAAAATAGC AAATCAGTGC ATGGACCCCT CTCTACAAGT GCAGCTTTTT
ATAGAAATTC TGAACAGATA TATCTATTTT TATGAAAAGG AAAATGATGC GGTAACAATT
CAGGTTTTAA ACCAGCTTAT CCAAAAGATT CGAGAAGACC TCCCGAATCT TGAATCCAGT
GAAGAAACAG AGCAGATTAA CAAACATTTT CATAACACAC TGGAGCATTT GCGCTTGCGG
CGGGAATCAC CAGAATCCGA GGGGCCAATT TATGAAGGTC TCATCCTTTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_060676.2
CDS100..2490
Misc Feature(1)40..42
Misc Feature(2)118..120
Misc Feature(3)142..2355
Misc Feature(4)172..231
Misc Feature(5)712..744
Misc Feature(6)1411..2487
Misc Feature(7)1597..2178
Misc Feature(8)2446..2448
Misc Feature(9)2470..2472
Exon (1)1..102
Exon (2)103..201
Exon (3)202..298
Exon (4)299..422
Exon (5)423..605
Exon (6)606..819
Exon (7)820..903
Exon (8)904..1013
Exon (9)1014..1110
Exon (10)1111..1259
Exon (11)1260..1467
Exon (12)1468..1623
Exon (13)1624..1746
Exon (14)1747..1926
Exon (15)1927..2166
Exon (16)2167..2310
Exon (17)2311..4983
Translation

Target ORF information:

RefSeq Version NM_018206.5
Organism Homo sapiens(Human)
Definition Homo sapiens VPS35, retromer complex component (VPS35), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_018206.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGCCTACAA CACAGCAGTC CCCTCAGGAT GAGCAGGAAA AGCTCTTGGA TGAAGCCATA 
CAGGCTGTGA AGGTCCAGTC ATTCCAAATG AAGAGATGCC TGGACAAAAA CAAGCTTATG
GATGCTCTAA AACATGCTTC TAATATGCTT GGTGAACTCC GGACTTCTAT GTTATCACCA
AAGAGTTACT ATGAACTTTA TATGGCCATT TCTGATGAAC TGCACTACTT GGAGGTCTAC
CTGACAGATG AGTTTGCTAA AGGAAGGAAA GTGGCAGATC TCTACGAACT TGTACAGTAT
GCTGGAAACA TTATCCCAAG GCTTTACCTT TTGATCACAG TTGGAGTTGT ATATGTCAAG
TCATTTCCTC AGTCCAGGAA GGATATTTTG AAAGATTTGG TAGAAATGTG CCGTGGTGTG
CAACATCCCT TGAGGGGTCT GTTTCTTCGA AATTACCTTC TTCAGTGTAC CAGAAATATC
TTACCTGATG AAGGAGAGCC AACAGATGAA GAAACAACTG GTGACATCAG TGATTCCATG
GATTTTGTAC TGCTCAACTT TGCAGAAATG AACAAGCTCT GGGTGCGAAT GCAGCATCAG
GGACATAGCC GAGATAGAGA AAAAAGAGAA CGAGAAAGAC AAGAACTGAG AATTTTAGTG
GGAACAAATT TGGTGCGCCT CAGTCAGTTG GAAGGTGTAA ATGTGGAACG TTACAAACAG
ATTGTTTTGA CTGGCATATT GGAGCAAGTT GTAAACTGTA GGGATGCTTT GGCTCAAGAA
TATCTCATGG AGTGTATTAT TCAGGTTTTC CCTGATGAAT TTCACCTCCA GACTTTGAAT
CCTTTTCTTC GGGCCTGTGC TGAGTTACAC CAGAATGTAA ATGTGAAGAA CATAATCATT
GCTTTAATTG ATAGATTAGC TTTATTTGCT CACCGTGAAG ATGGACCTGG AATCCCAGCG
GATATTAAAC TTTTTGATAT ATTTTCACAG CAGGTGGCTA CAGTGATACA GTCTAGACAA
GACATGCCTT CAGAGGATGT TGTATCTTTA CAAGTCTCTC TGATTAATCT TGCCATGAAA
TGTTACCCTG ATCGTGTGGA CTATGTTGAT AAAGTTCTAG AAACAACAGT GGAGATATTC
AATAAGCTCA ACCTTGAACA TATTGCTACC AGTAGTGCAG TTTCAAAGGA ACTCACCAGA
CTTTTGAAAA TACCAGTTGA CACTTACAAC AATATTTTAA CAGTCTTGAA ATTAAAACAT
TTTCACCCAC TCTTTGAGTA CTTTGACTAC GAGTCCAGAA AGAGCATGAG TTGTTATGTG
CTTAGTAATG TTCTGGATTA TAACACAGAA ATTGTCTCTC AAGACCAGGT GGATTCCATA
ATGAATTTGG TATCCACGTT GATTCAAGAT CAGCCAGATC AACCTGTAGA AGACCCTGAT
CCAGAAGATT TTGCTGATGA GCAGAGCCTT GTGGGCCGCT TCATTCATCT GCTGCGCTCT
GAGGACCCTG ACCAGCAGTA CTTGATTTTG AACACAGCAC GAAAACATTT TGGAGCTGGT
GGAAATCAGC GGATTCGCTT CACACTGCCA CCTTTGGTAT TTGCAGCTTA CCAGCTGGCT
TTTCGATATA AAGAGAATTC TAAAGTGGAT GACAAATGGG AAAAGAAATG CCAGAAGATT
TTTTCATTTG CCCACCAGAC TATCAGTGCT TTGATCAAAG CAGAGCTGGC AGAATTGCCC
TTAAGACTTT TTCTTCAAGG AGCACTAGCT GCTGGGGAAA TTGGTTTTGA AAATCATGAG
ACAGTCGCAT ATGAATTCAT GTCCCAGGCA TTTTCTCTGT ATGAAGATGA AATCAGCGAT
TCCAAAGCAC AGCTAGCTGC CATCACCTTG ATCATTGGCA CTTTTGAAAG GATGAAGTGC
TTCAGTGAAG AGAATCATGA ACCTCTGAGG ACTCAGTGTG CCCTTGCTGC ATCCAAACTT
CTAAAGAAAC CTGATCAGGG CCGAGCTGTG AGCACCTGTG CACATCTCTT CTGGTCTGGC
AGAAACACGG ACAAAAATGG GGAGGAGCTT CACGGAGGCA AGAGGGTAAT GGAGTGCCTA
AAAAAAGCTC TAAAAATAGC AAATCAGTGC ATGGACCCCT CTCTACAAGT GCAGCTTTTT
ATAGAAATTC TGAACAGATA TATCTATTTT TATGAAAAGG AAAATGATGC GGTAACAATT
CAGGTTTTAA ACCAGCTTAT CCAAAAGATT CGAGAAGACC TCCCGAATCT TGAATCCAGT
GAAGAAACAG AGCAGATTAA CAAACATTTT CATAACACAC TGGAGCATTT GCGCTTGCGG
CGGGAATCAC CAGAATCCGA GGGGCCAATT TATGAAGGTC TCATCCTTTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18510
Clone ID Related Accession (Same CDS sequence) NM_018206.5 , NM_018206.4
Accession Version NM_018206.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2391bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-06-25
Organism Homo sapiens(Human)
Product vacuolar protein sorting-associated protein 35
Comment

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGCCTACAA CACAGCAGTC CCCTCAGGAT GAGCAGGAAA AGCTCTTGGA TGAAGCCATA 
CAGGCTGTGA AGGTCCAGTC ATTCCAAATG AAGAGATGCC TGGACAAAAA CAAGCTTATG
GATGCTCTAA AACATGCTTC TAATATGCTT GGTGAACTCC GGACTTCTAT GTTATCACCA
AAGAGTTACT ATGAACTTTA TATGGCCATT TCTGATGAAC TGCACTACTT GGAGGTCTAC
CTGACAGATG AGTTTGCTAA AGGAAGGAAA GTGGCAGATC TCTACGAACT TGTACAGTAT
GCTGGAAACA TTATCCCAAG GCTTTACCTT TTGATCACAG TTGGAGTTGT ATATGTCAAG
TCATTTCCTC AGTCCAGGAA GGATATTTTG AAAGATTTGG TAGAAATGTG CCGTGGTGTG
CAACATCCCT TGAGGGGTCT GTTTCTTCGA AATTACCTTC TTCAGTGTAC CAGAAATATC
TTACCTGATG AAGGAGAGCC AACAGATGAA GAAACAACTG GTGACATCAG TGATTCCATG
GATTTTGTAC TGCTCAACTT TGCAGAAATG AACAAGCTCT GGGTGCGAAT GCAGCATCAG
GGACATAGCC GAGATAGAGA AAAAAGAGAA CGAGAAAGAC AAGAACTGAG AATTTTAGTG
GGAACAAATT TGGTGCGCCT CAGTCAGTTG GAAGGTGTAA ATGTGGAACG TTACAAACAG
ATTGTTTTGA CTGGCATATT GGAGCAAGTT GTAAACTGTA GGGATGCTTT GGCTCAAGAA
TATCTCATGG AGTGTATTAT TCAGGTTTTC CCTGATGAAT TTCACCTCCA GACTTTGAAT
CCTTTTCTTC GGGCCTGTGC TGAGTTACAC CAGAATGTAA ATGTGAAGAA CATAATCATT
GCTTTAATTG ATAGATTAGC TTTATTTGCT CACCGTGAAG ATGGACCTGG AATCCCAGCG
GATATTAAAC TTTTTGATAT ATTTTCACAG CAGGTGGCTA CAGTGATACA GTCTAGACAA
GACATGCCTT CAGAGGATGT TGTATCTTTA CAAGTCTCTC TGATTAATCT TGCCATGAAA
TGTTACCCTG ATCGTGTGGA CTATGTTGAT AAAGTTCTAG AAACAACAGT GGAGATATTC
AATAAGCTCA ACCTTGAACA TATTGCTACC AGTAGTGCAG TTTCAAAGGA ACTCACCAGA
CTTTTGAAAA TACCAGTTGA CACTTACAAC AATATTTTAA CAGTCTTGAA ATTAAAACAT
TTTCACCCAC TCTTTGAGTA CTTTGACTAC GAGTCCAGAA AGAGCATGAG TTGTTATGTG
CTTAGTAATG TTCTGGATTA TAACACAGAA ATTGTCTCTC AAGACCAGGT GGATTCCATA
ATGAATTTGG TATCCACGTT GATTCAAGAT CAGCCAGATC AACCTGTAGA AGACCCTGAT
CCAGAAGATT TTGCTGATGA GCAGAGCCTT GTGGGCCGCT TCATTCATCT GCTGCGCTCT
GAGGACCCTG ACCAGCAGTA CTTGATTTTG AACACAGCAC GAAAACATTT TGGAGCTGGT
GGAAATCAGC GGATTCGCTT CACACTGCCA CCTTTGGTAT TTGCAGCTTA CCAGCTGGCT
TTTCGATATA AAGAGAATTC TAAAGTGGAT GACAAATGGG AAAAGAAATG CCAGAAGATT
TTTTCATTTG CCCACCAGAC TATCAGTGCT TTGATCAAAG CAGAGCTGGC AGAATTGCCC
TTAAGACTTT TTCTTCAAGG AGCACTAGCT GCTGGGGAAA TTGGTTTTGA AAATCATGAG
ACAGTCGCAT ATGAATTCAT GTCCCAGGCA TTTTCTCTGT ATGAAGATGA AATCAGCGAT
TCCAAAGCAC AGCTAGCTGC CATCACCTTG ATCATTGGCA CTTTTGAAAG GATGAAGTGC
TTCAGTGAAG AGAATCATGA ACCTCTGAGG ACTCAGTGTG CCCTTGCTGC ATCCAAACTT
CTAAAGAAAC CTGATCAGGG CCGAGCTGTG AGCACCTGTG CACATCTCTT CTGGTCTGGC
AGAAACACGG ACAAAAATGG GGAGGAGCTT CACGGAGGCA AGAGGGTAAT GGAGTGCCTA
AAAAAAGCTC TAAAAATAGC AAATCAGTGC ATGGACCCCT CTCTACAAGT GCAGCTTTTT
ATAGAAATTC TGAACAGATA TATCTATTTT TATGAAAAGG AAAATGATGC GGTAACAATT
CAGGTTTTAA ACCAGCTTAT CCAAAAGATT CGAGAAGACC TCCCGAATCT TGAATCCAGT
GAAGAAACAG AGCAGATTAA CAAACATTTT CATAACACAC TGGAGCATTT GCGCTTGCGG
CGGGAATCAC CAGAATCCGA GGGGCCAATT TATGAAGGTC TCATCCTTTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_060676.2
Translation

Target ORF information:

RefSeq Version NM_018206.4
Organism Homo sapiens(Human)
Definition Homo sapiens VPS35 retromer complex component (VPS35), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_018206.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGCCTACAA CACAGCAGTC CCCTCAGGAT GAGCAGGAAA AGCTCTTGGA TGAAGCCATA 
CAGGCTGTGA AGGTCCAGTC ATTCCAAATG AAGAGATGCC TGGACAAAAA CAAGCTTATG
GATGCTCTAA AACATGCTTC TAATATGCTT GGTGAACTCC GGACTTCTAT GTTATCACCA
AAGAGTTACT ATGAACTTTA TATGGCCATT TCTGATGAAC TGCACTACTT GGAGGTCTAC
CTGACAGATG AGTTTGCTAA AGGAAGGAAA GTGGCAGATC TCTACGAACT TGTACAGTAT
GCTGGAAACA TTATCCCAAG GCTTTACCTT TTGATCACAG TTGGAGTTGT ATATGTCAAG
TCATTTCCTC AGTCCAGGAA GGATATTTTG AAAGATTTGG TAGAAATGTG CCGTGGTGTG
CAACATCCCT TGAGGGGTCT GTTTCTTCGA AATTACCTTC TTCAGTGTAC CAGAAATATC
TTACCTGATG AAGGAGAGCC AACAGATGAA GAAACAACTG GTGACATCAG TGATTCCATG
GATTTTGTAC TGCTCAACTT TGCAGAAATG AACAAGCTCT GGGTGCGAAT GCAGCATCAG
GGACATAGCC GAGATAGAGA AAAAAGAGAA CGAGAAAGAC AAGAACTGAG AATTTTAGTG
GGAACAAATT TGGTGCGCCT CAGTCAGTTG GAAGGTGTAA ATGTGGAACG TTACAAACAG
ATTGTTTTGA CTGGCATATT GGAGCAAGTT GTAAACTGTA GGGATGCTTT GGCTCAAGAA
TATCTCATGG AGTGTATTAT TCAGGTTTTC CCTGATGAAT TTCACCTCCA GACTTTGAAT
CCTTTTCTTC GGGCCTGTGC TGAGTTACAC CAGAATGTAA ATGTGAAGAA CATAATCATT
GCTTTAATTG ATAGATTAGC TTTATTTGCT CACCGTGAAG ATGGACCTGG AATCCCAGCG
GATATTAAAC TTTTTGATAT ATTTTCACAG CAGGTGGCTA CAGTGATACA GTCTAGACAA
GACATGCCTT CAGAGGATGT TGTATCTTTA CAAGTCTCTC TGATTAATCT TGCCATGAAA
TGTTACCCTG ATCGTGTGGA CTATGTTGAT AAAGTTCTAG AAACAACAGT GGAGATATTC
AATAAGCTCA ACCTTGAACA TATTGCTACC AGTAGTGCAG TTTCAAAGGA ACTCACCAGA
CTTTTGAAAA TACCAGTTGA CACTTACAAC AATATTTTAA CAGTCTTGAA ATTAAAACAT
TTTCACCCAC TCTTTGAGTA CTTTGACTAC GAGTCCAGAA AGAGCATGAG TTGTTATGTG
CTTAGTAATG TTCTGGATTA TAACACAGAA ATTGTCTCTC AAGACCAGGT GGATTCCATA
ATGAATTTGG TATCCACGTT GATTCAAGAT CAGCCAGATC AACCTGTAGA AGACCCTGAT
CCAGAAGATT TTGCTGATGA GCAGAGCCTT GTGGGCCGCT TCATTCATCT GCTGCGCTCT
GAGGACCCTG ACCAGCAGTA CTTGATTTTG AACACAGCAC GAAAACATTT TGGAGCTGGT
GGAAATCAGC GGATTCGCTT CACACTGCCA CCTTTGGTAT TTGCAGCTTA CCAGCTGGCT
TTTCGATATA AAGAGAATTC TAAAGTGGAT GACAAATGGG AAAAGAAATG CCAGAAGATT
TTTTCATTTG CCCACCAGAC TATCAGTGCT TTGATCAAAG CAGAGCTGGC AGAATTGCCC
TTAAGACTTT TTCTTCAAGG AGCACTAGCT GCTGGGGAAA TTGGTTTTGA AAATCATGAG
ACAGTCGCAT ATGAATTCAT GTCCCAGGCA TTTTCTCTGT ATGAAGATGA AATCAGCGAT
TCCAAAGCAC AGCTAGCTGC CATCACCTTG ATCATTGGCA CTTTTGAAAG GATGAAGTGC
TTCAGTGAAG AGAATCATGA ACCTCTGAGG ACTCAGTGTG CCCTTGCTGC ATCCAAACTT
CTAAAGAAAC CTGATCAGGG CCGAGCTGTG AGCACCTGTG CACATCTCTT CTGGTCTGGC
AGAAACACGG ACAAAAATGG GGAGGAGCTT CACGGAGGCA AGAGGGTAAT GGAGTGCCTA
AAAAAAGCTC TAAAAATAGC AAATCAGTGC ATGGACCCCT CTCTACAAGT GCAGCTTTTT
ATAGAAATTC TGAACAGATA TATCTATTTT TATGAAAAGG AAAATGATGC GGTAACAATT
CAGGTTTTAA ACCAGCTTAT CCAAAAGATT CGAGAAGACC TCCCGAATCT TGAATCCAGT
GAAGAAACAG AGCAGATTAA CAAACATTTT CATAACACAC TGGAGCATTT GCGCTTGCGG
CGGGAATCAC CAGAATCCGA GGGGCCAATT TATGAAGGTC TCATCCTTTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu49428
Clone ID Related Accession (Same CDS sequence) XM_005256045.3
Accession Version XM_005256045.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2190bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product vacuolar protein sorting-associated protein 35 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005256045.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCCATTT CTGATGAACT GCACTACTTG GAGGTCTACC TGACAGATGA GTTTGCTAAA 
GGAAGGAAAG TGGCAGATCT CTACGAACTT GTACAGTATG CTGGAAACAT TATCCCAAGG
CTTTACCTTT TGATCACAGT TGGAGTTGTA TATGTCAAGT CATTTCCTCA GTCCAGGAAG
GATATTTTGA AAGATTTGGT AGAAATGTGC CGTGGTGTGC AACATCCCTT GAGGGGTCTG
TTTCTTCGAA ATTACCTTCT TCAGTGTACC AGAAATATCT TACCTGATGA AGGAGAGCCA
ACAGATGAAG AAACAACTGG TGACATCAGT GATTCCATGG ATTTTGTACT GCTCAACTTT
GCAGAAATGA ACAAGCTCTG GGTGCGAATG CAGCATCAGG GACATAGCCG AGATAGAGAA
AAAAGAGAAC GAGAAAGACA AGAACTGAGA ATTTTAGTGG GAACAAATTT GGTGCGCCTC
AGTCAGTTGG AAGGTGTAAA TGTGGAACGT TACAAACAGA TTGTTTTGAC TGGCATATTG
GAGCAAGTTG TAAACTGTAG GGATGCTTTG GCTCAAGAAT ATCTCATGGA GTGTATTATT
CAGGTTTTCC CTGATGAATT TCACCTCCAG ACTTTGAATC CTTTTCTTCG GGCCTGTGCT
GAGTTACACC AGAATGTAAA TGTGAAGAAC ATAATCATTG CTTTAATTGA TAGATTAGCT
TTATTTGCTC ACCGTGAAGA TGGACCTGGA ATCCCAGCGG ATATTAAACT TTTTGATATA
TTTTCACAGC AGGTGGCTAC AGTGATACAG TCTAGACAAG ACATGCCTTC AGAGGATGTT
GTATCTTTAC AAGTCTCTCT GATTAATCTT GCCATGAAAT GTTACCCTGA TCGTGTGGAC
TATGTTGATA AAGTTCTAGA AACAACAGTG GAGATATTCA ATAAGCTCAA CCTTGAACAT
ATTGCTACCA GTAGTGCAGT TTCAAAGGAA CTCACCAGAC TTTTGAAAAT ACCAGTTGAC
ACTTACAACA ATATTTTAAC AGTCTTGAAA TTAAAACATT TTCACCCACT CTTTGAGTAC
TTTGACTACG AGTCCAGAAA GAGCATGAGT TGTTATGTGC TTAGTAATGT TCTGGATTAT
AACACAGAAA TTGTCTCTCA AGACCAGGTG GATTCCATAA TGAATTTGGT ATCCACGTTG
ATTCAAGATC AGCCAGATCA ACCTGTAGAA GACCCTGATC CAGAAGATTT TGCTGATGAG
CAGAGCCTTG TGGGCCGCTT CATTCATCTG CTGCGCTCTG AGGACCCTGA CCAGCAGTAC
TTGATTTTGA ACACAGCACG AAAACATTTT GGAGCTGGTG GAAATCAGCG GATTCGCTTC
ACACTGCCAC CTTTGGTATT TGCAGCTTAC CAGCTGGCTT TTCGATATAA AGAGAATTCT
AAAGTGGATG ACAAATGGGA AAAGAAATGC CAGAAGATTT TTTCATTTGC CCACCAGACT
ATCAGTGCTT TGATCAAAGC AGAGCTGGCA GAATTGCCCT TAAGACTTTT TCTTCAAGGA
GCACTAGCTG CTGGGGAAAT TGGTTTTGAA AATCATGAGA CAGTCGCATA TGAATTCATG
TCCCAGGCAT TTTCTCTGTA TGAAGATGAA ATCAGCGATT CCAAAGCACA GCTAGCTGCC
ATCACCTTGA TCATTGGCAC TTTTGAAAGG ATGAAGTGCT TCAGTGAAGA GAATCACGAA
CCTCTGAGGA CTCAGTGTGC CCTTGCTGCA TCCAAACTTC TAAAGAAACC TGATCAGGGC
CGAGCTGTGA GCACCTGTGC ACATCTCTTC TGGTCTGGCA GAAACACGGA CAAAAATGGG
GAGGAGCTTC ACGGAGGCAA GAGGGTAATG GAGTGCCTAA AAAAAGCTCT AAAAATAGCA
AATCAGTGCA TGGACCCCTC TCTACAAGTG CAGCTTTTTA TAGAAATTCT GAACAGATAT
ATCTATTTTT ATGAAAAGGA AAATGATGCG GTAACAATTC AGGTTTTAAA CCAGCTTATC
CAAAAGATTC GAGAAGACCT CCCGAATCTT GAATCCAGTG AAGAAACAGA GCAGATTAAC
AAACATTTTC ATAACACACT GGAGCATTTG CGCTTGCGGC GGGAATCACC AGAATCCGAG
GGGCCAATTT ATGAAGGTCT CATCCTTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005256102.1
CDS186..2375
Misc Feature(1)186..2240
Translation

Target ORF information:

RefSeq Version XM_005256045.3
Organism Homo sapiens(Human)
Definition Homo sapiens VPS35 retromer complex component (VPS35), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005256045.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCCATTT CTGATGAACT GCACTACTTG GAGGTCTACC TGACAGATGA GTTTGCTAAA 
GGAAGGAAAG TGGCAGATCT CTACGAACTT GTACAGTATG CTGGAAACAT TATCCCAAGG
CTTTACCTTT TGATCACAGT TGGAGTTGTA TATGTCAAGT CATTTCCTCA GTCCAGGAAG
GATATTTTGA AAGATTTGGT AGAAATGTGC CGTGGTGTGC AACATCCCTT GAGGGGTCTG
TTTCTTCGAA ATTACCTTCT TCAGTGTACC AGAAATATCT TACCTGATGA AGGAGAGCCA
ACAGATGAAG AAACAACTGG TGACATCAGT GATTCCATGG ATTTTGTACT GCTCAACTTT
GCAGAAATGA ACAAGCTCTG GGTGCGAATG CAGCATCAGG GACATAGCCG AGATAGAGAA
AAAAGAGAAC GAGAAAGACA AGAACTGAGA ATTTTAGTGG GAACAAATTT GGTGCGCCTC
AGTCAGTTGG AAGGTGTAAA TGTGGAACGT TACAAACAGA TTGTTTTGAC TGGCATATTG
GAGCAAGTTG TAAACTGTAG GGATGCTTTG GCTCAAGAAT ATCTCATGGA GTGTATTATT
CAGGTTTTCC CTGATGAATT TCACCTCCAG ACTTTGAATC CTTTTCTTCG GGCCTGTGCT
GAGTTACACC AGAATGTAAA TGTGAAGAAC ATAATCATTG CTTTAATTGA TAGATTAGCT
TTATTTGCTC ACCGTGAAGA TGGACCTGGA ATCCCAGCGG ATATTAAACT TTTTGATATA
TTTTCACAGC AGGTGGCTAC AGTGATACAG TCTAGACAAG ACATGCCTTC AGAGGATGTT
GTATCTTTAC AAGTCTCTCT GATTAATCTT GCCATGAAAT GTTACCCTGA TCGTGTGGAC
TATGTTGATA AAGTTCTAGA AACAACAGTG GAGATATTCA ATAAGCTCAA CCTTGAACAT
ATTGCTACCA GTAGTGCAGT TTCAAAGGAA CTCACCAGAC TTTTGAAAAT ACCAGTTGAC
ACTTACAACA ATATTTTAAC AGTCTTGAAA TTAAAACATT TTCACCCACT CTTTGAGTAC
TTTGACTACG AGTCCAGAAA GAGCATGAGT TGTTATGTGC TTAGTAATGT TCTGGATTAT
AACACAGAAA TTGTCTCTCA AGACCAGGTG GATTCCATAA TGAATTTGGT ATCCACGTTG
ATTCAAGATC AGCCAGATCA ACCTGTAGAA GACCCTGATC CAGAAGATTT TGCTGATGAG
CAGAGCCTTG TGGGCCGCTT CATTCATCTG CTGCGCTCTG AGGACCCTGA CCAGCAGTAC
TTGATTTTGA ACACAGCACG AAAACATTTT GGAGCTGGTG GAAATCAGCG GATTCGCTTC
ACACTGCCAC CTTTGGTATT TGCAGCTTAC CAGCTGGCTT TTCGATATAA AGAGAATTCT
AAAGTGGATG ACAAATGGGA AAAGAAATGC CAGAAGATTT TTTCATTTGC CCACCAGACT
ATCAGTGCTT TGATCAAAGC AGAGCTGGCA GAATTGCCCT TAAGACTTTT TCTTCAAGGA
GCACTAGCTG CTGGGGAAAT TGGTTTTGAA AATCATGAGA CAGTCGCATA TGAATTCATG
TCCCAGGCAT TTTCTCTGTA TGAAGATGAA ATCAGCGATT CCAAAGCACA GCTAGCTGCC
ATCACCTTGA TCATTGGCAC TTTTGAAAGG ATGAAGTGCT TCAGTGAAGA GAATCACGAA
CCTCTGAGGA CTCAGTGTGC CCTTGCTGCA TCCAAACTTC TAAAGAAACC TGATCAGGGC
CGAGCTGTGA GCACCTGTGC ACATCTCTTC TGGTCTGGCA GAAACACGGA CAAAAATGGG
GAGGAGCTTC ACGGAGGCAA GAGGGTAATG GAGTGCCTAA AAAAAGCTCT AAAAATAGCA
AATCAGTGCA TGGACCCCTC TCTACAAGTG CAGCTTTTTA TAGAAATTCT GAACAGATAT
ATCTATTTTT ATGAAAAGGA AAATGATGCG GTAACAATTC AGGTTTTAAA CCAGCTTATC
CAAAAGATTC GAGAAGACCT CCCGAATCTT GAATCCAGTG AAGAAACAGA GCAGATTAAC
AAACATTTTC ATAACACACT GGAGCATTTG CGCTTGCGGC GGGAATCACC AGAATCCGAG
GGGCCAATTT ATGAAGGTCT CATCCTTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu69690
Clone ID Related Accession (Same CDS sequence) XM_011523227.2
Accession Version XM_011523227.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2304bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product vacuolar protein sorting-associated protein 35 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011523227.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGAAGAGAT GCCTGGACAA AAACAAGCTT ATGGATGCTC TAAAACATGC TTCTAATATG 
CTTGGTGAAC TCCGGACTTC TATGTTATCA CCAAAGAGTT ACTATGAACT TTATATGGCC
ATTTCTGATG AACTGCACTA CTTGGAGGTC TACCTGACAG ATGAGTTTGC TAAAGGAAGG
AAAGTGGCAG ATCTCTACGA ACTTGTACAG TATGCTGGAA ACATTATCCC AAGGCTTTAC
CTTTTGATCA CAGTTGGAGT TGTATATGTC AAGTCATTTC CTCAGTCCAG GAAGGATATT
TTGAAAGATT TGGTAGAAAT GTGCCGTGGT GTGCAACATC CCTTGAGGGG TCTGTTTCTT
CGAAATTACC TTCTTCAGTG TACCAGAAAT ATCTTACCTG ATGAAGGAGA GCCAACAGAT
GAAGAAACAA CTGGTGACAT CAGTGATTCC ATGGATTTTG TACTGCTCAA CTTTGCAGAA
ATGAACAAGC TCTGGGTGCG AATGCAGCAT CAGGGACATA GCCGAGATAG AGAAAAAAGA
GAACGAGAAA GACAAGAACT GAGAATTTTA GTGGGAACAA ATTTGGTGCG CCTCAGTCAG
TTGGAAGGTG TAAATGTGGA ACGTTACAAA CAGATTGTTT TGACTGGCAT ATTGGAGCAA
GTTGTAAACT GTAGGGATGC TTTGGCTCAA GAATATCTCA TGGAGTGTAT TATTCAGGTT
TTCCCTGATG AATTTCACCT CCAGACTTTG AATCCTTTTC TTCGGGCCTG TGCTGAGTTA
CACCAGAATG TAAATGTGAA GAACATAATC ATTGCTTTAA TTGATAGATT AGCTTTATTT
GCTCACCGTG AAGATGGACC TGGAATCCCA GCGGATATTA AACTTTTTGA TATATTTTCA
CAGCAGGTGG CTACAGTGAT ACAGTCTAGA CAAGACATGC CTTCAGAGGA TGTTGTATCT
TTACAAGTCT CTCTGATTAA TCTTGCCATG AAATGTTACC CTGATCGTGT GGACTATGTT
GATAAAGTTC TAGAAACAAC AGTGGAGATA TTCAATAAGC TCAACCTTGA ACATATTGCT
ACCAGTAGTG CAGTTTCAAA GGAACTCACC AGACTTTTGA AAATACCAGT TGACACTTAC
AACAATATTT TAACAGTCTT GAAATTAAAA CATTTTCACC CACTCTTTGA GTACTTTGAC
TACGAGTCCA GAAAGAGCAT GAGTTGTTAT GTGCTTAGTA ATGTTCTGGA TTATAACACA
GAAATTGTCT CTCAAGACCA GGTGGATTCC ATAATGAATT TGGTATCCAC GTTGATTCAA
GATCAGCCAG ATCAACCTGT AGAAGACCCT GATCCAGAAG ATTTTGCTGA TGAGCAGAGC
CTTGTGGGCC GCTTCATTCA TCTGCTGCGC TCTGAGGACC CTGACCAGCA GTACTTGATT
TTGAACACAG CACGAAAACA TTTTGGAGCT GGTGGAAATC AGCGGATTCG CTTCACACTG
CCACCTTTGG TATTTGCAGC TTACCAGCTG GCTTTTCGAT ATAAAGAGAA TTCTAAAGTG
GATGACAAAT GGGAAAAGAA ATGCCAGAAG ATTTTTTCAT TTGCCCACCA GACTATCAGT
GCTTTGATCA AAGCAGAGCT GGCAGAATTG CCCTTAAGAC TTTTTCTTCA AGGAGCACTA
GCTGCTGGGG AAATTGGTTT TGAAAATCAT GAGACAGTCG CATATGAATT CATGTCCCAG
GCATTTTCTC TGTATGAAGA TGAAATCAGC GATTCCAAAG CACAGCTAGC TGCCATCACC
TTGATCATTG GCACTTTTGA AAGGATGAAG TGCTTCAGTG AAGAGAATCA CGAACCTCTG
AGGACTCAGT GTGCCCTTGC TGCATCCAAA CTTCTAAAGA AACCTGATCA GGGCCGAGCT
GTGAGCACCT GTGCACATCT CTTCTGGTCT GGCAGAAACA CGGACAAAAA TGGGGAGGAG
CTTCACGGAG GCAAGAGGGT AATGGAGTGC CTAAAAAAAG CTCTAAAAAT AGCAAATCAG
TGCATGGACC CCTCTCTACA AGTGCAGCTT TTTATAGAAA TTCTGAACAG ATATATCTAT
TTTTATGAAA AGGAAAATGA TGCGGTAACA ATTCAGGTTT TAAACCAGCT TATCCAAAAG
ATTCGAGAAG ACCTCCCGAA TCTTGAATCC AGTGAAGAAA CAGAGCAGAT TAACAAACAT
TTTCATAACA CACTGGAGCA TTTGCGCTTG CGGCGGGAAT CACCAGAATC CGAGGGGCCA
ATTTATGAAG GTCTCATCCT TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011521529.1
CDS917..3220
Misc Feature(1)917..3085
Translation

Target ORF information:

RefSeq Version XM_011523227.2
Organism Homo sapiens(Human)
Definition Homo sapiens VPS35 retromer complex component (VPS35), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011523227.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGAAGAGAT GCCTGGACAA AAACAAGCTT ATGGATGCTC TAAAACATGC TTCTAATATG 
CTTGGTGAAC TCCGGACTTC TATGTTATCA CCAAAGAGTT ACTATGAACT TTATATGGCC
ATTTCTGATG AACTGCACTA CTTGGAGGTC TACCTGACAG ATGAGTTTGC TAAAGGAAGG
AAAGTGGCAG ATCTCTACGA ACTTGTACAG TATGCTGGAA ACATTATCCC AAGGCTTTAC
CTTTTGATCA CAGTTGGAGT TGTATATGTC AAGTCATTTC CTCAGTCCAG GAAGGATATT
TTGAAAGATT TGGTAGAAAT GTGCCGTGGT GTGCAACATC CCTTGAGGGG TCTGTTTCTT
CGAAATTACC TTCTTCAGTG TACCAGAAAT ATCTTACCTG ATGAAGGAGA GCCAACAGAT
GAAGAAACAA CTGGTGACAT CAGTGATTCC ATGGATTTTG TACTGCTCAA CTTTGCAGAA
ATGAACAAGC TCTGGGTGCG AATGCAGCAT CAGGGACATA GCCGAGATAG AGAAAAAAGA
GAACGAGAAA GACAAGAACT GAGAATTTTA GTGGGAACAA ATTTGGTGCG CCTCAGTCAG
TTGGAAGGTG TAAATGTGGA ACGTTACAAA CAGATTGTTT TGACTGGCAT ATTGGAGCAA
GTTGTAAACT GTAGGGATGC TTTGGCTCAA GAATATCTCA TGGAGTGTAT TATTCAGGTT
TTCCCTGATG AATTTCACCT CCAGACTTTG AATCCTTTTC TTCGGGCCTG TGCTGAGTTA
CACCAGAATG TAAATGTGAA GAACATAATC ATTGCTTTAA TTGATAGATT AGCTTTATTT
GCTCACCGTG AAGATGGACC TGGAATCCCA GCGGATATTA AACTTTTTGA TATATTTTCA
CAGCAGGTGG CTACAGTGAT ACAGTCTAGA CAAGACATGC CTTCAGAGGA TGTTGTATCT
TTACAAGTCT CTCTGATTAA TCTTGCCATG AAATGTTACC CTGATCGTGT GGACTATGTT
GATAAAGTTC TAGAAACAAC AGTGGAGATA TTCAATAAGC TCAACCTTGA ACATATTGCT
ACCAGTAGTG CAGTTTCAAA GGAACTCACC AGACTTTTGA AAATACCAGT TGACACTTAC
AACAATATTT TAACAGTCTT GAAATTAAAA CATTTTCACC CACTCTTTGA GTACTTTGAC
TACGAGTCCA GAAAGAGCAT GAGTTGTTAT GTGCTTAGTA ATGTTCTGGA TTATAACACA
GAAATTGTCT CTCAAGACCA GGTGGATTCC ATAATGAATT TGGTATCCAC GTTGATTCAA
GATCAGCCAG ATCAACCTGT AGAAGACCCT GATCCAGAAG ATTTTGCTGA TGAGCAGAGC
CTTGTGGGCC GCTTCATTCA TCTGCTGCGC TCTGAGGACC CTGACCAGCA GTACTTGATT
TTGAACACAG CACGAAAACA TTTTGGAGCT GGTGGAAATC AGCGGATTCG CTTCACACTG
CCACCTTTGG TATTTGCAGC TTACCAGCTG GCTTTTCGAT ATAAAGAGAA TTCTAAAGTG
GATGACAAAT GGGAAAAGAA ATGCCAGAAG ATTTTTTCAT TTGCCCACCA GACTATCAGT
GCTTTGATCA AAGCAGAGCT GGCAGAATTG CCCTTAAGAC TTTTTCTTCA AGGAGCACTA
GCTGCTGGGG AAATTGGTTT TGAAAATCAT GAGACAGTCG CATATGAATT CATGTCCCAG
GCATTTTCTC TGTATGAAGA TGAAATCAGC GATTCCAAAG CACAGCTAGC TGCCATCACC
TTGATCATTG GCACTTTTGA AAGGATGAAG TGCTTCAGTG AAGAGAATCA CGAACCTCTG
AGGACTCAGT GTGCCCTTGC TGCATCCAAA CTTCTAAAGA AACCTGATCA GGGCCGAGCT
GTGAGCACCT GTGCACATCT CTTCTGGTCT GGCAGAAACA CGGACAAAAA TGGGGAGGAG
CTTCACGGAG GCAAGAGGGT AATGGAGTGC CTAAAAAAAG CTCTAAAAAT AGCAAATCAG
TGCATGGACC CCTCTCTACA AGTGCAGCTT TTTATAGAAA TTCTGAACAG ATATATCTAT
TTTTATGAAA AGGAAAATGA TGCGGTAACA ATTCAGGTTT TAAACCAGCT TATCCAAAAG
ATTCGAGAAG ACCTCCCGAA TCTTGAATCC AGTGAAGAAA CAGAGCAGAT TAACAAACAT
TTTCATAACA CACTGGAGCA TTTGCGCTTG CGGCGGGAAT CACCAGAATC CGAGGGGCCA
ATTTATGAAG GTCTCATCCT TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

VPS35 regulates cell surface recycling and signaling of dopamine receptor D1.
Neurobiology of aging4622-31(2016 Oct)
Wang C,Niu M,Zhou Z,Zheng X,Zhang L,Tian Y,Yu X,Bu G,Xu H,Ma Q,Zhang YW


book

Parkinson Disease-linked Vps35 R524W Mutation Impairs the Endosomal Association of Retromer and Induces α-Synuclein Aggregation.
The Journal of biological chemistry291(35)18283-98(2016 Aug)
Follett J,Bugarcic A,Yang Z,Ariotti N,Norwood SJ,Collins BM,Parton RG,Teasdale RD


book

Parkinson's disease-associated mutant VPS35 causes mitochondrial dysfunction by recycling DLP1 complexes.
Nature medicine22(1)54-63(2016 Jan)
Wang W,Wang X,Fujioka H,Hoppel C,Whone AL,Caldwell MA,Cullen PJ,Liu J,Zhu X


book

An assessment of the frequency of mutations in the GBA and VPS35 genes in Hungarian patients with sporadic Parkinson's disease.
Neuroscience letters610135-8(2016 Jan)
Török R,Zádori D,Török N,Csility É,Vécsei L,Klivényi P


book

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Cell163(3)712-23(2015 Oct)
Hein MY,Hubner NC,Poser I,Cox J,Nagaraj N,Toyoda Y,Gak IA,Weisswange I,Mansfeld J,Buchholz F,Hyman AA,Mann M


 
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