VPS35 cDNA ORF clone, Homo sapiens(human)

The following VPS35 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS35 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu18510 NM_018206.5
Latest version!
Homo sapiens VPS35 retromer complex component (VPS35), mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu49428 XM_005256045.3
Latest version!
Homo sapiens VPS35 retromer complex component (VPS35), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu69690 XM_011523227.3
Latest version!
Homo sapiens VPS35 retromer complex component (VPS35), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu108594 NM_018206.6
Latest version!
Homo sapiens VPS35 retromer complex component (VPS35), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
View Old Accession Versions >>
OHu18510 NM_018206.4 Homo sapiens VPS35 retromer complex component (VPS35), mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu69690 XM_011523227.2 Homo sapiens VPS35 retromer complex component (VPS35), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu18510
    Clone ID Related Accession (Same CDS sequence) NM_018206.5 , NM_018206.4
    Accession Version NM_018206.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2391bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-24
    Organism Homo sapiens(human)
    Product vacuolar protein sorting-associated protein 35
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB049686.1, BC093036.1, BQ574179.1, AC012186.8 and BU621624.1. This sequence is a reference standard in the RefSeqGene project. On Apr 15, 2016 this sequence version replaced NM_018206.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.1800.1, SRR1803613.283710.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCTACAA CACAGCAGTC CCCTCAGGAT GAGCAGGAAA AGCTCTTGGA TGAAGCCATA 
    CAGGCTGTGA AGGTCCAGTC ATTCCAAATG AAGAGATGCC TGGACAAAAA CAAGCTTATG
    GATGCTCTAA AACATGCTTC TAATATGCTT GGTGAACTCC GGACTTCTAT GTTATCACCA
    AAGAGTTACT ATGAACTTTA TATGGCCATT TCTGATGAAC TGCACTACTT GGAGGTCTAC
    CTGACAGATG AGTTTGCTAA AGGAAGGAAA GTGGCAGATC TCTACGAACT TGTACAGTAT
    GCTGGAAACA TTATCCCAAG GCTTTACCTT TTGATCACAG TTGGAGTTGT ATATGTCAAG
    TCATTTCCTC AGTCCAGGAA GGATATTTTG AAAGATTTGG TAGAAATGTG CCGTGGTGTG
    CAACATCCCT TGAGGGGTCT GTTTCTTCGA AATTACCTTC TTCAGTGTAC CAGAAATATC
    TTACCTGATG AAGGAGAGCC AACAGATGAA GAAACAACTG GTGACATCAG TGATTCCATG
    GATTTTGTAC TGCTCAACTT TGCAGAAATG AACAAGCTCT GGGTGCGAAT GCAGCATCAG
    GGACATAGCC GAGATAGAGA AAAAAGAGAA CGAGAAAGAC AAGAACTGAG AATTTTAGTG
    GGAACAAATT TGGTGCGCCT CAGTCAGTTG GAAGGTGTAA ATGTGGAACG TTACAAACAG
    ATTGTTTTGA CTGGCATATT GGAGCAAGTT GTAAACTGTA GGGATGCTTT GGCTCAAGAA
    TATCTCATGG AGTGTATTAT TCAGGTTTTC CCTGATGAAT TTCACCTCCA GACTTTGAAT
    CCTTTTCTTC GGGCCTGTGC TGAGTTACAC CAGAATGTAA ATGTGAAGAA CATAATCATT
    GCTTTAATTG ATAGATTAGC TTTATTTGCT CACCGTGAAG ATGGACCTGG AATCCCAGCG
    GATATTAAAC TTTTTGATAT ATTTTCACAG CAGGTGGCTA CAGTGATACA GTCTAGACAA
    GACATGCCTT CAGAGGATGT TGTATCTTTA CAAGTCTCTC TGATTAATCT TGCCATGAAA
    TGTTACCCTG ATCGTGTGGA CTATGTTGAT AAAGTTCTAG AAACAACAGT GGAGATATTC
    AATAAGCTCA ACCTTGAACA TATTGCTACC AGTAGTGCAG TTTCAAAGGA ACTCACCAGA
    CTTTTGAAAA TACCAGTTGA CACTTACAAC AATATTTTAA CAGTCTTGAA ATTAAAACAT
    TTTCACCCAC TCTTTGAGTA CTTTGACTAC GAGTCCAGAA AGAGCATGAG TTGTTATGTG
    CTTAGTAATG TTCTGGATTA TAACACAGAA ATTGTCTCTC AAGACCAGGT GGATTCCATA
    ATGAATTTGG TATCCACGTT GATTCAAGAT CAGCCAGATC AACCTGTAGA AGACCCTGAT
    CCAGAAGATT TTGCTGATGA GCAGAGCCTT GTGGGCCGCT TCATTCATCT GCTGCGCTCT
    GAGGACCCTG ACCAGCAGTA CTTGATTTTG AACACAGCAC GAAAACATTT TGGAGCTGGT
    GGAAATCAGC GGATTCGCTT CACACTGCCA CCTTTGGTAT TTGCAGCTTA CCAGCTGGCT
    TTTCGATATA AAGAGAATTC TAAAGTGGAT GACAAATGGG AAAAGAAATG CCAGAAGATT
    TTTTCATTTG CCCACCAGAC TATCAGTGCT TTGATCAAAG CAGAGCTGGC AGAATTGCCC
    TTAAGACTTT TTCTTCAAGG AGCACTAGCT GCTGGGGAAA TTGGTTTTGA AAATCATGAG
    ACAGTCGCAT ATGAATTCAT GTCCCAGGCA TTTTCTCTGT ATGAAGATGA AATCAGCGAT
    TCCAAAGCAC AGCTAGCTGC CATCACCTTG ATCATTGGCA CTTTTGAAAG GATGAAGTGC
    TTCAGTGAAG AGAATCATGA ACCTCTGAGG ACTCAGTGTG CCCTTGCTGC ATCCAAACTT
    CTAAAGAAAC CTGATCAGGG CCGAGCTGTG AGCACCTGTG CACATCTCTT CTGGTCTGGC
    AGAAACACGG ACAAAAATGG GGAGGAGCTT CACGGAGGCA AGAGGGTAAT GGAGTGCCTA
    AAAAAAGCTC TAAAAATAGC AAATCAGTGC ATGGACCCCT CTCTACAAGT GCAGCTTTTT
    ATAGAAATTC TGAACAGATA TATCTATTTT TATGAAAAGG AAAATGATGC GGTAACAATT
    CAGGTTTTAA ACCAGCTTAT CCAAAAGATT CGAGAAGACC TCCCGAATCT TGAATCCAGT
    GAAGAAACAG AGCAGATTAA CAAACATTTT CATAACACAC TGGAGCATTT GCGCTTGCGG
    CGGGAATCAC CAGAATCCGA GGGGCCAATT TATGAAGGTC TCATCCTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_060676.2
    CDS100..2490
    Translation

    Target ORF information:

    RefSeq Version NM_018206.5
    Organism Homo sapiens(human)
    Definition Homo sapiens VPS35 retromer complex component (VPS35), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_018206.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCTACAA CACAGCAGTC CCCTCAGGAT GAGCAGGAAA AGCTCTTGGA TGAAGCCATA 
    CAGGCTGTGA AGGTCCAGTC ATTCCAAATG AAGAGATGCC TGGACAAAAA CAAGCTTATG
    GATGCTCTAA AACATGCTTC TAATATGCTT GGTGAACTCC GGACTTCTAT GTTATCACCA
    AAGAGTTACT ATGAACTTTA TATGGCCATT TCTGATGAAC TGCACTACTT GGAGGTCTAC
    CTGACAGATG AGTTTGCTAA AGGAAGGAAA GTGGCAGATC TCTACGAACT TGTACAGTAT
    GCTGGAAACA TTATCCCAAG GCTTTACCTT TTGATCACAG TTGGAGTTGT ATATGTCAAG
    TCATTTCCTC AGTCCAGGAA GGATATTTTG AAAGATTTGG TAGAAATGTG CCGTGGTGTG
    CAACATCCCT TGAGGGGTCT GTTTCTTCGA AATTACCTTC TTCAGTGTAC CAGAAATATC
    TTACCTGATG AAGGAGAGCC AACAGATGAA GAAACAACTG GTGACATCAG TGATTCCATG
    GATTTTGTAC TGCTCAACTT TGCAGAAATG AACAAGCTCT GGGTGCGAAT GCAGCATCAG
    GGACATAGCC GAGATAGAGA AAAAAGAGAA CGAGAAAGAC AAGAACTGAG AATTTTAGTG
    GGAACAAATT TGGTGCGCCT CAGTCAGTTG GAAGGTGTAA ATGTGGAACG TTACAAACAG
    ATTGTTTTGA CTGGCATATT GGAGCAAGTT GTAAACTGTA GGGATGCTTT GGCTCAAGAA
    TATCTCATGG AGTGTATTAT TCAGGTTTTC CCTGATGAAT TTCACCTCCA GACTTTGAAT
    CCTTTTCTTC GGGCCTGTGC TGAGTTACAC CAGAATGTAA ATGTGAAGAA CATAATCATT
    GCTTTAATTG ATAGATTAGC TTTATTTGCT CACCGTGAAG ATGGACCTGG AATCCCAGCG
    GATATTAAAC TTTTTGATAT ATTTTCACAG CAGGTGGCTA CAGTGATACA GTCTAGACAA
    GACATGCCTT CAGAGGATGT TGTATCTTTA CAAGTCTCTC TGATTAATCT TGCCATGAAA
    TGTTACCCTG ATCGTGTGGA CTATGTTGAT AAAGTTCTAG AAACAACAGT GGAGATATTC
    AATAAGCTCA ACCTTGAACA TATTGCTACC AGTAGTGCAG TTTCAAAGGA ACTCACCAGA
    CTTTTGAAAA TACCAGTTGA CACTTACAAC AATATTTTAA CAGTCTTGAA ATTAAAACAT
    TTTCACCCAC TCTTTGAGTA CTTTGACTAC GAGTCCAGAA AGAGCATGAG TTGTTATGTG
    CTTAGTAATG TTCTGGATTA TAACACAGAA ATTGTCTCTC AAGACCAGGT GGATTCCATA
    ATGAATTTGG TATCCACGTT GATTCAAGAT CAGCCAGATC AACCTGTAGA AGACCCTGAT
    CCAGAAGATT TTGCTGATGA GCAGAGCCTT GTGGGCCGCT TCATTCATCT GCTGCGCTCT
    GAGGACCCTG ACCAGCAGTA CTTGATTTTG AACACAGCAC GAAAACATTT TGGAGCTGGT
    GGAAATCAGC GGATTCGCTT CACACTGCCA CCTTTGGTAT TTGCAGCTTA CCAGCTGGCT
    TTTCGATATA AAGAGAATTC TAAAGTGGAT GACAAATGGG AAAAGAAATG CCAGAAGATT
    TTTTCATTTG CCCACCAGAC TATCAGTGCT TTGATCAAAG CAGAGCTGGC AGAATTGCCC
    TTAAGACTTT TTCTTCAAGG AGCACTAGCT GCTGGGGAAA TTGGTTTTGA AAATCATGAG
    ACAGTCGCAT ATGAATTCAT GTCCCAGGCA TTTTCTCTGT ATGAAGATGA AATCAGCGAT
    TCCAAAGCAC AGCTAGCTGC CATCACCTTG ATCATTGGCA CTTTTGAAAG GATGAAGTGC
    TTCAGTGAAG AGAATCATGA ACCTCTGAGG ACTCAGTGTG CCCTTGCTGC ATCCAAACTT
    CTAAAGAAAC CTGATCAGGG CCGAGCTGTG AGCACCTGTG CACATCTCTT CTGGTCTGGC
    AGAAACACGG ACAAAAATGG GGAGGAGCTT CACGGAGGCA AGAGGGTAAT GGAGTGCCTA
    AAAAAAGCTC TAAAAATAGC AAATCAGTGC ATGGACCCCT CTCTACAAGT GCAGCTTTTT
    ATAGAAATTC TGAACAGATA TATCTATTTT TATGAAAAGG AAAATGATGC GGTAACAATT
    CAGGTTTTAA ACCAGCTTAT CCAAAAGATT CGAGAAGACC TCCCGAATCT TGAATCCAGT
    GAAGAAACAG AGCAGATTAA CAAACATTTT CATAACACAC TGGAGCATTT GCGCTTGCGG
    CGGGAATCAC CAGAATCCGA GGGGCCAATT TATGAAGGTC TCATCCTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18510
    Clone ID Related Accession (Same CDS sequence) NM_018206.5 , NM_018206.4
    Accession Version NM_018206.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2391bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-06-24
    Organism Homo sapiens(Human)
    Product vacuolar protein sorting-associated protein 35
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCTACAA CACAGCAGTC CCCTCAGGAT GAGCAGGAAA AGCTCTTGGA TGAAGCCATA 
    CAGGCTGTGA AGGTCCAGTC ATTCCAAATG AAGAGATGCC TGGACAAAAA CAAGCTTATG
    GATGCTCTAA AACATGCTTC TAATATGCTT GGTGAACTCC GGACTTCTAT GTTATCACCA
    AAGAGTTACT ATGAACTTTA TATGGCCATT TCTGATGAAC TGCACTACTT GGAGGTCTAC
    CTGACAGATG AGTTTGCTAA AGGAAGGAAA GTGGCAGATC TCTACGAACT TGTACAGTAT
    GCTGGAAACA TTATCCCAAG GCTTTACCTT TTGATCACAG TTGGAGTTGT ATATGTCAAG
    TCATTTCCTC AGTCCAGGAA GGATATTTTG AAAGATTTGG TAGAAATGTG CCGTGGTGTG
    CAACATCCCT TGAGGGGTCT GTTTCTTCGA AATTACCTTC TTCAGTGTAC CAGAAATATC
    TTACCTGATG AAGGAGAGCC AACAGATGAA GAAACAACTG GTGACATCAG TGATTCCATG
    GATTTTGTAC TGCTCAACTT TGCAGAAATG AACAAGCTCT GGGTGCGAAT GCAGCATCAG
    GGACATAGCC GAGATAGAGA AAAAAGAGAA CGAGAAAGAC AAGAACTGAG AATTTTAGTG
    GGAACAAATT TGGTGCGCCT CAGTCAGTTG GAAGGTGTAA ATGTGGAACG TTACAAACAG
    ATTGTTTTGA CTGGCATATT GGAGCAAGTT GTAAACTGTA GGGATGCTTT GGCTCAAGAA
    TATCTCATGG AGTGTATTAT TCAGGTTTTC CCTGATGAAT TTCACCTCCA GACTTTGAAT
    CCTTTTCTTC GGGCCTGTGC TGAGTTACAC CAGAATGTAA ATGTGAAGAA CATAATCATT
    GCTTTAATTG ATAGATTAGC TTTATTTGCT CACCGTGAAG ATGGACCTGG AATCCCAGCG
    GATATTAAAC TTTTTGATAT ATTTTCACAG CAGGTGGCTA CAGTGATACA GTCTAGACAA
    GACATGCCTT CAGAGGATGT TGTATCTTTA CAAGTCTCTC TGATTAATCT TGCCATGAAA
    TGTTACCCTG ATCGTGTGGA CTATGTTGAT AAAGTTCTAG AAACAACAGT GGAGATATTC
    AATAAGCTCA ACCTTGAACA TATTGCTACC AGTAGTGCAG TTTCAAAGGA ACTCACCAGA
    CTTTTGAAAA TACCAGTTGA CACTTACAAC AATATTTTAA CAGTCTTGAA ATTAAAACAT
    TTTCACCCAC TCTTTGAGTA CTTTGACTAC GAGTCCAGAA AGAGCATGAG TTGTTATGTG
    CTTAGTAATG TTCTGGATTA TAACACAGAA ATTGTCTCTC AAGACCAGGT GGATTCCATA
    ATGAATTTGG TATCCACGTT GATTCAAGAT CAGCCAGATC AACCTGTAGA AGACCCTGAT
    CCAGAAGATT TTGCTGATGA GCAGAGCCTT GTGGGCCGCT TCATTCATCT GCTGCGCTCT
    GAGGACCCTG ACCAGCAGTA CTTGATTTTG AACACAGCAC GAAAACATTT TGGAGCTGGT
    GGAAATCAGC GGATTCGCTT CACACTGCCA CCTTTGGTAT TTGCAGCTTA CCAGCTGGCT
    TTTCGATATA AAGAGAATTC TAAAGTGGAT GACAAATGGG AAAAGAAATG CCAGAAGATT
    TTTTCATTTG CCCACCAGAC TATCAGTGCT TTGATCAAAG CAGAGCTGGC AGAATTGCCC
    TTAAGACTTT TTCTTCAAGG AGCACTAGCT GCTGGGGAAA TTGGTTTTGA AAATCATGAG
    ACAGTCGCAT ATGAATTCAT GTCCCAGGCA TTTTCTCTGT ATGAAGATGA AATCAGCGAT
    TCCAAAGCAC AGCTAGCTGC CATCACCTTG ATCATTGGCA CTTTTGAAAG GATGAAGTGC
    TTCAGTGAAG AGAATCATGA ACCTCTGAGG ACTCAGTGTG CCCTTGCTGC ATCCAAACTT
    CTAAAGAAAC CTGATCAGGG CCGAGCTGTG AGCACCTGTG CACATCTCTT CTGGTCTGGC
    AGAAACACGG ACAAAAATGG GGAGGAGCTT CACGGAGGCA AGAGGGTAAT GGAGTGCCTA
    AAAAAAGCTC TAAAAATAGC AAATCAGTGC ATGGACCCCT CTCTACAAGT GCAGCTTTTT
    ATAGAAATTC TGAACAGATA TATCTATTTT TATGAAAAGG AAAATGATGC GGTAACAATT
    CAGGTTTTAA ACCAGCTTAT CCAAAAGATT CGAGAAGACC TCCCGAATCT TGAATCCAGT
    GAAGAAACAG AGCAGATTAA CAAACATTTT CATAACACAC TGGAGCATTT GCGCTTGCGG
    CGGGAATCAC CAGAATCCGA GGGGCCAATT TATGAAGGTC TCATCCTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_060676.2
    Translation

    Target ORF information:

    RefSeq Version NM_018206.4
    Organism Homo sapiens(Human)
    Definition Homo sapiens VPS35 retromer complex component (VPS35), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_018206.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCTACAA CACAGCAGTC CCCTCAGGAT GAGCAGGAAA AGCTCTTGGA TGAAGCCATA 
    CAGGCTGTGA AGGTCCAGTC ATTCCAAATG AAGAGATGCC TGGACAAAAA CAAGCTTATG
    GATGCTCTAA AACATGCTTC TAATATGCTT GGTGAACTCC GGACTTCTAT GTTATCACCA
    AAGAGTTACT ATGAACTTTA TATGGCCATT TCTGATGAAC TGCACTACTT GGAGGTCTAC
    CTGACAGATG AGTTTGCTAA AGGAAGGAAA GTGGCAGATC TCTACGAACT TGTACAGTAT
    GCTGGAAACA TTATCCCAAG GCTTTACCTT TTGATCACAG TTGGAGTTGT ATATGTCAAG
    TCATTTCCTC AGTCCAGGAA GGATATTTTG AAAGATTTGG TAGAAATGTG CCGTGGTGTG
    CAACATCCCT TGAGGGGTCT GTTTCTTCGA AATTACCTTC TTCAGTGTAC CAGAAATATC
    TTACCTGATG AAGGAGAGCC AACAGATGAA GAAACAACTG GTGACATCAG TGATTCCATG
    GATTTTGTAC TGCTCAACTT TGCAGAAATG AACAAGCTCT GGGTGCGAAT GCAGCATCAG
    GGACATAGCC GAGATAGAGA AAAAAGAGAA CGAGAAAGAC AAGAACTGAG AATTTTAGTG
    GGAACAAATT TGGTGCGCCT CAGTCAGTTG GAAGGTGTAA ATGTGGAACG TTACAAACAG
    ATTGTTTTGA CTGGCATATT GGAGCAAGTT GTAAACTGTA GGGATGCTTT GGCTCAAGAA
    TATCTCATGG AGTGTATTAT TCAGGTTTTC CCTGATGAAT TTCACCTCCA GACTTTGAAT
    CCTTTTCTTC GGGCCTGTGC TGAGTTACAC CAGAATGTAA ATGTGAAGAA CATAATCATT
    GCTTTAATTG ATAGATTAGC TTTATTTGCT CACCGTGAAG ATGGACCTGG AATCCCAGCG
    GATATTAAAC TTTTTGATAT ATTTTCACAG CAGGTGGCTA CAGTGATACA GTCTAGACAA
    GACATGCCTT CAGAGGATGT TGTATCTTTA CAAGTCTCTC TGATTAATCT TGCCATGAAA
    TGTTACCCTG ATCGTGTGGA CTATGTTGAT AAAGTTCTAG AAACAACAGT GGAGATATTC
    AATAAGCTCA ACCTTGAACA TATTGCTACC AGTAGTGCAG TTTCAAAGGA ACTCACCAGA
    CTTTTGAAAA TACCAGTTGA CACTTACAAC AATATTTTAA CAGTCTTGAA ATTAAAACAT
    TTTCACCCAC TCTTTGAGTA CTTTGACTAC GAGTCCAGAA AGAGCATGAG TTGTTATGTG
    CTTAGTAATG TTCTGGATTA TAACACAGAA ATTGTCTCTC AAGACCAGGT GGATTCCATA
    ATGAATTTGG TATCCACGTT GATTCAAGAT CAGCCAGATC AACCTGTAGA AGACCCTGAT
    CCAGAAGATT TTGCTGATGA GCAGAGCCTT GTGGGCCGCT TCATTCATCT GCTGCGCTCT
    GAGGACCCTG ACCAGCAGTA CTTGATTTTG AACACAGCAC GAAAACATTT TGGAGCTGGT
    GGAAATCAGC GGATTCGCTT CACACTGCCA CCTTTGGTAT TTGCAGCTTA CCAGCTGGCT
    TTTCGATATA AAGAGAATTC TAAAGTGGAT GACAAATGGG AAAAGAAATG CCAGAAGATT
    TTTTCATTTG CCCACCAGAC TATCAGTGCT TTGATCAAAG CAGAGCTGGC AGAATTGCCC
    TTAAGACTTT TTCTTCAAGG AGCACTAGCT GCTGGGGAAA TTGGTTTTGA AAATCATGAG
    ACAGTCGCAT ATGAATTCAT GTCCCAGGCA TTTTCTCTGT ATGAAGATGA AATCAGCGAT
    TCCAAAGCAC AGCTAGCTGC CATCACCTTG ATCATTGGCA CTTTTGAAAG GATGAAGTGC
    TTCAGTGAAG AGAATCATGA ACCTCTGAGG ACTCAGTGTG CCCTTGCTGC ATCCAAACTT
    CTAAAGAAAC CTGATCAGGG CCGAGCTGTG AGCACCTGTG CACATCTCTT CTGGTCTGGC
    AGAAACACGG ACAAAAATGG GGAGGAGCTT CACGGAGGCA AGAGGGTAAT GGAGTGCCTA
    AAAAAAGCTC TAAAAATAGC AAATCAGTGC ATGGACCCCT CTCTACAAGT GCAGCTTTTT
    ATAGAAATTC TGAACAGATA TATCTATTTT TATGAAAAGG AAAATGATGC GGTAACAATT
    CAGGTTTTAA ACCAGCTTAT CCAAAAGATT CGAGAAGACC TCCCGAATCT TGAATCCAGT
    GAAGAAACAG AGCAGATTAA CAAACATTTT CATAACACAC TGGAGCATTT GCGCTTGCGG
    CGGGAATCAC CAGAATCCGA GGGGCCAATT TATGAAGGTC TCATCCTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu49428
    Clone ID Related Accession (Same CDS sequence) XM_005256045.3
    Accession Version XM_005256045.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2190bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product vacuolar protein sorting-associated protein 35 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005256045.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCATTT CTGATGAACT GCACTACTTG GAGGTCTACC TGACAGATGA GTTTGCTAAA 
    GGAAGGAAAG TGGCAGATCT CTACGAACTT GTACAGTATG CTGGAAACAT TATCCCAAGG
    CTTTACCTTT TGATCACAGT TGGAGTTGTA TATGTCAAGT CATTTCCTCA GTCCAGGAAG
    GATATTTTGA AAGATTTGGT AGAAATGTGC CGTGGTGTGC AACATCCCTT GAGGGGTCTG
    TTTCTTCGAA ATTACCTTCT TCAGTGTACC AGAAATATCT TACCTGATGA AGGAGAGCCA
    ACAGATGAAG AAACAACTGG TGACATCAGT GATTCCATGG ATTTTGTACT GCTCAACTTT
    GCAGAAATGA ACAAGCTCTG GGTGCGAATG CAGCATCAGG GACATAGCCG AGATAGAGAA
    AAAAGAGAAC GAGAAAGACA AGAACTGAGA ATTTTAGTGG GAACAAATTT GGTGCGCCTC
    AGTCAGTTGG AAGGTGTAAA TGTGGAACGT TACAAACAGA TTGTTTTGAC TGGCATATTG
    GAGCAAGTTG TAAACTGTAG GGATGCTTTG GCTCAAGAAT ATCTCATGGA GTGTATTATT
    CAGGTTTTCC CTGATGAATT TCACCTCCAG ACTTTGAATC CTTTTCTTCG GGCCTGTGCT
    GAGTTACACC AGAATGTAAA TGTGAAGAAC ATAATCATTG CTTTAATTGA TAGATTAGCT
    TTATTTGCTC ACCGTGAAGA TGGACCTGGA ATCCCAGCGG ATATTAAACT TTTTGATATA
    TTTTCACAGC AGGTGGCTAC AGTGATACAG TCTAGACAAG ACATGCCTTC AGAGGATGTT
    GTATCTTTAC AAGTCTCTCT GATTAATCTT GCCATGAAAT GTTACCCTGA TCGTGTGGAC
    TATGTTGATA AAGTTCTAGA AACAACAGTG GAGATATTCA ATAAGCTCAA CCTTGAACAT
    ATTGCTACCA GTAGTGCAGT TTCAAAGGAA CTCACCAGAC TTTTGAAAAT ACCAGTTGAC
    ACTTACAACA ATATTTTAAC AGTCTTGAAA TTAAAACATT TTCACCCACT CTTTGAGTAC
    TTTGACTACG AGTCCAGAAA GAGCATGAGT TGTTATGTGC TTAGTAATGT TCTGGATTAT
    AACACAGAAA TTGTCTCTCA AGACCAGGTG GATTCCATAA TGAATTTGGT ATCCACGTTG
    ATTCAAGATC AGCCAGATCA ACCTGTAGAA GACCCTGATC CAGAAGATTT TGCTGATGAG
    CAGAGCCTTG TGGGCCGCTT CATTCATCTG CTGCGCTCTG AGGACCCTGA CCAGCAGTAC
    TTGATTTTGA ACACAGCACG AAAACATTTT GGAGCTGGTG GAAATCAGCG GATTCGCTTC
    ACACTGCCAC CTTTGGTATT TGCAGCTTAC CAGCTGGCTT TTCGATATAA AGAGAATTCT
    AAAGTGGATG ACAAATGGGA AAAGAAATGC CAGAAGATTT TTTCATTTGC CCACCAGACT
    ATCAGTGCTT TGATCAAAGC AGAGCTGGCA GAATTGCCCT TAAGACTTTT TCTTCAAGGA
    GCACTAGCTG CTGGGGAAAT TGGTTTTGAA AATCATGAGA CAGTCGCATA TGAATTCATG
    TCCCAGGCAT TTTCTCTGTA TGAAGATGAA ATCAGCGATT CCAAAGCACA GCTAGCTGCC
    ATCACCTTGA TCATTGGCAC TTTTGAAAGG ATGAAGTGCT TCAGTGAAGA GAATCACGAA
    CCTCTGAGGA CTCAGTGTGC CCTTGCTGCA TCCAAACTTC TAAAGAAACC TGATCAGGGC
    CGAGCTGTGA GCACCTGTGC ACATCTCTTC TGGTCTGGCA GAAACACGGA CAAAAATGGG
    GAGGAGCTTC ACGGAGGCAA GAGGGTAATG GAGTGCCTAA AAAAAGCTCT AAAAATAGCA
    AATCAGTGCA TGGACCCCTC TCTACAAGTG CAGCTTTTTA TAGAAATTCT GAACAGATAT
    ATCTATTTTT ATGAAAAGGA AAATGATGCG GTAACAATTC AGGTTTTAAA CCAGCTTATC
    CAAAAGATTC GAGAAGACCT CCCGAATCTT GAATCCAGTG AAGAAACAGA GCAGATTAAC
    AAACATTTTC ATAACACACT GGAGCATTTG CGCTTGCGGC GGGAATCACC AGAATCCGAG
    GGGCCAATTT ATGAAGGTCT CATCCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005256102.1
    CDS186..2375
    Translation

    Target ORF information:

    RefSeq Version XM_005256045.3
    Organism Homo sapiens(human)
    Definition Homo sapiens VPS35 retromer complex component (VPS35), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005256045.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCATTT CTGATGAACT GCACTACTTG GAGGTCTACC TGACAGATGA GTTTGCTAAA 
    GGAAGGAAAG TGGCAGATCT CTACGAACTT GTACAGTATG CTGGAAACAT TATCCCAAGG
    CTTTACCTTT TGATCACAGT TGGAGTTGTA TATGTCAAGT CATTTCCTCA GTCCAGGAAG
    GATATTTTGA AAGATTTGGT AGAAATGTGC CGTGGTGTGC AACATCCCTT GAGGGGTCTG
    TTTCTTCGAA ATTACCTTCT TCAGTGTACC AGAAATATCT TACCTGATGA AGGAGAGCCA
    ACAGATGAAG AAACAACTGG TGACATCAGT GATTCCATGG ATTTTGTACT GCTCAACTTT
    GCAGAAATGA ACAAGCTCTG GGTGCGAATG CAGCATCAGG GACATAGCCG AGATAGAGAA
    AAAAGAGAAC GAGAAAGACA AGAACTGAGA ATTTTAGTGG GAACAAATTT GGTGCGCCTC
    AGTCAGTTGG AAGGTGTAAA TGTGGAACGT TACAAACAGA TTGTTTTGAC TGGCATATTG
    GAGCAAGTTG TAAACTGTAG GGATGCTTTG GCTCAAGAAT ATCTCATGGA GTGTATTATT
    CAGGTTTTCC CTGATGAATT TCACCTCCAG ACTTTGAATC CTTTTCTTCG GGCCTGTGCT
    GAGTTACACC AGAATGTAAA TGTGAAGAAC ATAATCATTG CTTTAATTGA TAGATTAGCT
    TTATTTGCTC ACCGTGAAGA TGGACCTGGA ATCCCAGCGG ATATTAAACT TTTTGATATA
    TTTTCACAGC AGGTGGCTAC AGTGATACAG TCTAGACAAG ACATGCCTTC AGAGGATGTT
    GTATCTTTAC AAGTCTCTCT GATTAATCTT GCCATGAAAT GTTACCCTGA TCGTGTGGAC
    TATGTTGATA AAGTTCTAGA AACAACAGTG GAGATATTCA ATAAGCTCAA CCTTGAACAT
    ATTGCTACCA GTAGTGCAGT TTCAAAGGAA CTCACCAGAC TTTTGAAAAT ACCAGTTGAC
    ACTTACAACA ATATTTTAAC AGTCTTGAAA TTAAAACATT TTCACCCACT CTTTGAGTAC
    TTTGACTACG AGTCCAGAAA GAGCATGAGT TGTTATGTGC TTAGTAATGT TCTGGATTAT
    AACACAGAAA TTGTCTCTCA AGACCAGGTG GATTCCATAA TGAATTTGGT ATCCACGTTG
    ATTCAAGATC AGCCAGATCA ACCTGTAGAA GACCCTGATC CAGAAGATTT TGCTGATGAG
    CAGAGCCTTG TGGGCCGCTT CATTCATCTG CTGCGCTCTG AGGACCCTGA CCAGCAGTAC
    TTGATTTTGA ACACAGCACG AAAACATTTT GGAGCTGGTG GAAATCAGCG GATTCGCTTC
    ACACTGCCAC CTTTGGTATT TGCAGCTTAC CAGCTGGCTT TTCGATATAA AGAGAATTCT
    AAAGTGGATG ACAAATGGGA AAAGAAATGC CAGAAGATTT TTTCATTTGC CCACCAGACT
    ATCAGTGCTT TGATCAAAGC AGAGCTGGCA GAATTGCCCT TAAGACTTTT TCTTCAAGGA
    GCACTAGCTG CTGGGGAAAT TGGTTTTGAA AATCATGAGA CAGTCGCATA TGAATTCATG
    TCCCAGGCAT TTTCTCTGTA TGAAGATGAA ATCAGCGATT CCAAAGCACA GCTAGCTGCC
    ATCACCTTGA TCATTGGCAC TTTTGAAAGG ATGAAGTGCT TCAGTGAAGA GAATCACGAA
    CCTCTGAGGA CTCAGTGTGC CCTTGCTGCA TCCAAACTTC TAAAGAAACC TGATCAGGGC
    CGAGCTGTGA GCACCTGTGC ACATCTCTTC TGGTCTGGCA GAAACACGGA CAAAAATGGG
    GAGGAGCTTC ACGGAGGCAA GAGGGTAATG GAGTGCCTAA AAAAAGCTCT AAAAATAGCA
    AATCAGTGCA TGGACCCCTC TCTACAAGTG CAGCTTTTTA TAGAAATTCT GAACAGATAT
    ATCTATTTTT ATGAAAAGGA AAATGATGCG GTAACAATTC AGGTTTTAAA CCAGCTTATC
    CAAAAGATTC GAGAAGACCT CCCGAATCTT GAATCCAGTG AAGAAACAGA GCAGATTAAC
    AAACATTTTC ATAACACACT GGAGCATTTG CGCTTGCGGC GGGAATCACC AGAATCCGAG
    GGGCCAATTT ATGAAGGTCT CATCCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu69690
    Clone ID Related Accession (Same CDS sequence) XM_011523227.2 , XM_011523227.3
    Accession Version XM_011523227.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2304bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product vacuolar protein sorting-associated protein 35 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011523227.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAAGAGAT GCCTGGACAA AAACAAGCTT ATGGATGCTC TAAAACATGC TTCTAATATG 
    CTTGGTGAAC TCCGGACTTC TATGTTATCA CCAAAGAGTT ACTATGAACT TTATATGGCC
    ATTTCTGATG AACTGCACTA CTTGGAGGTC TACCTGACAG ATGAGTTTGC TAAAGGAAGG
    AAAGTGGCAG ATCTCTACGA ACTTGTACAG TATGCTGGAA ACATTATCCC AAGGCTTTAC
    CTTTTGATCA CAGTTGGAGT TGTATATGTC AAGTCATTTC CTCAGTCCAG GAAGGATATT
    TTGAAAGATT TGGTAGAAAT GTGCCGTGGT GTGCAACATC CCTTGAGGGG TCTGTTTCTT
    CGAAATTACC TTCTTCAGTG TACCAGAAAT ATCTTACCTG ATGAAGGAGA GCCAACAGAT
    GAAGAAACAA CTGGTGACAT CAGTGATTCC ATGGATTTTG TACTGCTCAA CTTTGCAGAA
    ATGAACAAGC TCTGGGTGCG AATGCAGCAT CAGGGACATA GCCGAGATAG AGAAAAAAGA
    GAACGAGAAA GACAAGAACT GAGAATTTTA GTGGGAACAA ATTTGGTGCG CCTCAGTCAG
    TTGGAAGGTG TAAATGTGGA ACGTTACAAA CAGATTGTTT TGACTGGCAT ATTGGAGCAA
    GTTGTAAACT GTAGGGATGC TTTGGCTCAA GAATATCTCA TGGAGTGTAT TATTCAGGTT
    TTCCCTGATG AATTTCACCT CCAGACTTTG AATCCTTTTC TTCGGGCCTG TGCTGAGTTA
    CACCAGAATG TAAATGTGAA GAACATAATC ATTGCTTTAA TTGATAGATT AGCTTTATTT
    GCTCACCGTG AAGATGGACC TGGAATCCCA GCGGATATTA AACTTTTTGA TATATTTTCA
    CAGCAGGTGG CTACAGTGAT ACAGTCTAGA CAAGACATGC CTTCAGAGGA TGTTGTATCT
    TTACAAGTCT CTCTGATTAA TCTTGCCATG AAATGTTACC CTGATCGTGT GGACTATGTT
    GATAAAGTTC TAGAAACAAC AGTGGAGATA TTCAATAAGC TCAACCTTGA ACATATTGCT
    ACCAGTAGTG CAGTTTCAAA GGAACTCACC AGACTTTTGA AAATACCAGT TGACACTTAC
    AACAATATTT TAACAGTCTT GAAATTAAAA CATTTTCACC CACTCTTTGA GTACTTTGAC
    TACGAGTCCA GAAAGAGCAT GAGTTGTTAT GTGCTTAGTA ATGTTCTGGA TTATAACACA
    GAAATTGTCT CTCAAGACCA GGTGGATTCC ATAATGAATT TGGTATCCAC GTTGATTCAA
    GATCAGCCAG ATCAACCTGT AGAAGACCCT GATCCAGAAG ATTTTGCTGA TGAGCAGAGC
    CTTGTGGGCC GCTTCATTCA TCTGCTGCGC TCTGAGGACC CTGACCAGCA GTACTTGATT
    TTGAACACAG CACGAAAACA TTTTGGAGCT GGTGGAAATC AGCGGATTCG CTTCACACTG
    CCACCTTTGG TATTTGCAGC TTACCAGCTG GCTTTTCGAT ATAAAGAGAA TTCTAAAGTG
    GATGACAAAT GGGAAAAGAA ATGCCAGAAG ATTTTTTCAT TTGCCCACCA GACTATCAGT
    GCTTTGATCA AAGCAGAGCT GGCAGAATTG CCCTTAAGAC TTTTTCTTCA AGGAGCACTA
    GCTGCTGGGG AAATTGGTTT TGAAAATCAT GAGACAGTCG CATATGAATT CATGTCCCAG
    GCATTTTCTC TGTATGAAGA TGAAATCAGC GATTCCAAAG CACAGCTAGC TGCCATCACC
    TTGATCATTG GCACTTTTGA AAGGATGAAG TGCTTCAGTG AAGAGAATCA CGAACCTCTG
    AGGACTCAGT GTGCCCTTGC TGCATCCAAA CTTCTAAAGA AACCTGATCA GGGCCGAGCT
    GTGAGCACCT GTGCACATCT CTTCTGGTCT GGCAGAAACA CGGACAAAAA TGGGGAGGAG
    CTTCACGGAG GCAAGAGGGT AATGGAGTGC CTAAAAAAAG CTCTAAAAAT AGCAAATCAG
    TGCATGGACC CCTCTCTACA AGTGCAGCTT TTTATAGAAA TTCTGAACAG ATATATCTAT
    TTTTATGAAA AGGAAAATGA TGCGGTAACA ATTCAGGTTT TAAACCAGCT TATCCAAAAG
    ATTCGAGAAG ACCTCCCGAA TCTTGAATCC AGTGAAGAAA CAGAGCAGAT TAACAAACAT
    TTTCATAACA CACTGGAGCA TTTGCGCTTG CGGCGGGAAT CACCAGAATC CGAGGGGCCA
    ATTTATGAAG GTCTCATCCT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011521529.1
    CDS917..3220
    Translation

    Target ORF information:

    RefSeq Version XM_011523227.2
    Organism Homo sapiens(human)
    Definition Homo sapiens VPS35 retromer complex component (VPS35), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011523227.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAAGAGAT GCCTGGACAA AAACAAGCTT ATGGATGCTC TAAAACATGC TTCTAATATG 
    CTTGGTGAAC TCCGGACTTC TATGTTATCA CCAAAGAGTT ACTATGAACT TTATATGGCC
    ATTTCTGATG AACTGCACTA CTTGGAGGTC TACCTGACAG ATGAGTTTGC TAAAGGAAGG
    AAAGTGGCAG ATCTCTACGA ACTTGTACAG TATGCTGGAA ACATTATCCC AAGGCTTTAC
    CTTTTGATCA CAGTTGGAGT TGTATATGTC AAGTCATTTC CTCAGTCCAG GAAGGATATT
    TTGAAAGATT TGGTAGAAAT GTGCCGTGGT GTGCAACATC CCTTGAGGGG TCTGTTTCTT
    CGAAATTACC TTCTTCAGTG TACCAGAAAT ATCTTACCTG ATGAAGGAGA GCCAACAGAT
    GAAGAAACAA CTGGTGACAT CAGTGATTCC ATGGATTTTG TACTGCTCAA CTTTGCAGAA
    ATGAACAAGC TCTGGGTGCG AATGCAGCAT CAGGGACATA GCCGAGATAG AGAAAAAAGA
    GAACGAGAAA GACAAGAACT GAGAATTTTA GTGGGAACAA ATTTGGTGCG CCTCAGTCAG
    TTGGAAGGTG TAAATGTGGA ACGTTACAAA CAGATTGTTT TGACTGGCAT ATTGGAGCAA
    GTTGTAAACT GTAGGGATGC TTTGGCTCAA GAATATCTCA TGGAGTGTAT TATTCAGGTT
    TTCCCTGATG AATTTCACCT CCAGACTTTG AATCCTTTTC TTCGGGCCTG TGCTGAGTTA
    CACCAGAATG TAAATGTGAA GAACATAATC ATTGCTTTAA TTGATAGATT AGCTTTATTT
    GCTCACCGTG AAGATGGACC TGGAATCCCA GCGGATATTA AACTTTTTGA TATATTTTCA
    CAGCAGGTGG CTACAGTGAT ACAGTCTAGA CAAGACATGC CTTCAGAGGA TGTTGTATCT
    TTACAAGTCT CTCTGATTAA TCTTGCCATG AAATGTTACC CTGATCGTGT GGACTATGTT
    GATAAAGTTC TAGAAACAAC AGTGGAGATA TTCAATAAGC TCAACCTTGA ACATATTGCT
    ACCAGTAGTG CAGTTTCAAA GGAACTCACC AGACTTTTGA AAATACCAGT TGACACTTAC
    AACAATATTT TAACAGTCTT GAAATTAAAA CATTTTCACC CACTCTTTGA GTACTTTGAC
    TACGAGTCCA GAAAGAGCAT GAGTTGTTAT GTGCTTAGTA ATGTTCTGGA TTATAACACA
    GAAATTGTCT CTCAAGACCA GGTGGATTCC ATAATGAATT TGGTATCCAC GTTGATTCAA
    GATCAGCCAG ATCAACCTGT AGAAGACCCT GATCCAGAAG ATTTTGCTGA TGAGCAGAGC
    CTTGTGGGCC GCTTCATTCA TCTGCTGCGC TCTGAGGACC CTGACCAGCA GTACTTGATT
    TTGAACACAG CACGAAAACA TTTTGGAGCT GGTGGAAATC AGCGGATTCG CTTCACACTG
    CCACCTTTGG TATTTGCAGC TTACCAGCTG GCTTTTCGAT ATAAAGAGAA TTCTAAAGTG
    GATGACAAAT GGGAAAAGAA ATGCCAGAAG ATTTTTTCAT TTGCCCACCA GACTATCAGT
    GCTTTGATCA AAGCAGAGCT GGCAGAATTG CCCTTAAGAC TTTTTCTTCA AGGAGCACTA
    GCTGCTGGGG AAATTGGTTT TGAAAATCAT GAGACAGTCG CATATGAATT CATGTCCCAG
    GCATTTTCTC TGTATGAAGA TGAAATCAGC GATTCCAAAG CACAGCTAGC TGCCATCACC
    TTGATCATTG GCACTTTTGA AAGGATGAAG TGCTTCAGTG AAGAGAATCA CGAACCTCTG
    AGGACTCAGT GTGCCCTTGC TGCATCCAAA CTTCTAAAGA AACCTGATCA GGGCCGAGCT
    GTGAGCACCT GTGCACATCT CTTCTGGTCT GGCAGAAACA CGGACAAAAA TGGGGAGGAG
    CTTCACGGAG GCAAGAGGGT AATGGAGTGC CTAAAAAAAG CTCTAAAAAT AGCAAATCAG
    TGCATGGACC CCTCTCTACA AGTGCAGCTT TTTATAGAAA TTCTGAACAG ATATATCTAT
    TTTTATGAAA AGGAAAATGA TGCGGTAACA ATTCAGGTTT TAAACCAGCT TATCCAAAAG
    ATTCGAGAAG ACCTCCCGAA TCTTGAATCC AGTGAAGAAA CAGAGCAGAT TAACAAACAT
    TTTCATAACA CACTGGAGCA TTTGCGCTTG CGGCGGGAAT CACCAGAATC CGAGGGGCCA
    ATTTATGAAG GTCTCATCCT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu69690
    Clone ID Related Accession (Same CDS sequence) XM_011523227.2 , XM_011523227.3
    Accession Version XM_011523227.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2304bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product vacuolar protein sorting-associated protein 35 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011523227.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAAGAGAT GCCTGGACAA AAACAAGCTT ATGGATGCTC TAAAACATGC TTCTAATATG 
    CTTGGTGAAC TCCGGACTTC TATGTTATCA CCAAAGAGTT ACTATGAACT TTATATGGCC
    ATTTCTGATG AACTGCACTA CTTGGAGGTC TACCTGACAG ATGAGTTTGC TAAAGGAAGG
    AAAGTGGCAG ATCTCTACGA ACTTGTACAG TATGCTGGAA ACATTATCCC AAGGCTTTAC
    CTTTTGATCA CAGTTGGAGT TGTATATGTC AAGTCATTTC CTCAGTCCAG GAAGGATATT
    TTGAAAGATT TGGTAGAAAT GTGCCGTGGT GTGCAACATC CCTTGAGGGG TCTGTTTCTT
    CGAAATTACC TTCTTCAGTG TACCAGAAAT ATCTTACCTG ATGAAGGAGA GCCAACAGAT
    GAAGAAACAA CTGGTGACAT CAGTGATTCC ATGGATTTTG TACTGCTCAA CTTTGCAGAA
    ATGAACAAGC TCTGGGTGCG AATGCAGCAT CAGGGACATA GCCGAGATAG AGAAAAAAGA
    GAACGAGAAA GACAAGAACT GAGAATTTTA GTGGGAACAA ATTTGGTGCG CCTCAGTCAG
    TTGGAAGGTG TAAATGTGGA ACGTTACAAA CAGATTGTTT TGACTGGCAT ATTGGAGCAA
    GTTGTAAACT GTAGGGATGC TTTGGCTCAA GAATATCTCA TGGAGTGTAT TATTCAGGTT
    TTCCCTGATG AATTTCACCT CCAGACTTTG AATCCTTTTC TTCGGGCCTG TGCTGAGTTA
    CACCAGAATG TAAATGTGAA GAACATAATC ATTGCTTTAA TTGATAGATT AGCTTTATTT
    GCTCACCGTG AAGATGGACC TGGAATCCCA GCGGATATTA AACTTTTTGA TATATTTTCA
    CAGCAGGTGG CTACAGTGAT ACAGTCTAGA CAAGACATGC CTTCAGAGGA TGTTGTATCT
    TTACAAGTCT CTCTGATTAA TCTTGCCATG AAATGTTACC CTGATCGTGT GGACTATGTT
    GATAAAGTTC TAGAAACAAC AGTGGAGATA TTCAATAAGC TCAACCTTGA ACATATTGCT
    ACCAGTAGTG CAGTTTCAAA GGAACTCACC AGACTTTTGA AAATACCAGT TGACACTTAC
    AACAATATTT TAACAGTCTT GAAATTAAAA CATTTTCACC CACTCTTTGA GTACTTTGAC
    TACGAGTCCA GAAAGAGCAT GAGTTGTTAT GTGCTTAGTA ATGTTCTGGA TTATAACACA
    GAAATTGTCT CTCAAGACCA GGTGGATTCC ATAATGAATT TGGTATCCAC GTTGATTCAA
    GATCAGCCAG ATCAACCTGT AGAAGACCCT GATCCAGAAG ATTTTGCTGA TGAGCAGAGC
    CTTGTGGGCC GCTTCATTCA TCTGCTGCGC TCTGAGGACC CTGACCAGCA GTACTTGATT
    TTGAACACAG CACGAAAACA TTTTGGAGCT GGTGGAAATC AGCGGATTCG CTTCACACTG
    CCACCTTTGG TATTTGCAGC TTACCAGCTG GCTTTTCGAT ATAAAGAGAA TTCTAAAGTG
    GATGACAAAT GGGAAAAGAA ATGCCAGAAG ATTTTTTCAT TTGCCCACCA GACTATCAGT
    GCTTTGATCA AAGCAGAGCT GGCAGAATTG CCCTTAAGAC TTTTTCTTCA AGGAGCACTA
    GCTGCTGGGG AAATTGGTTT TGAAAATCAT GAGACAGTCG CATATGAATT CATGTCCCAG
    GCATTTTCTC TGTATGAAGA TGAAATCAGC GATTCCAAAG CACAGCTAGC TGCCATCACC
    TTGATCATTG GCACTTTTGA AAGGATGAAG TGCTTCAGTG AAGAGAATCA CGAACCTCTG
    AGGACTCAGT GTGCCCTTGC TGCATCCAAA CTTCTAAAGA AACCTGATCA GGGCCGAGCT
    GTGAGCACCT GTGCACATCT CTTCTGGTCT GGCAGAAACA CGGACAAAAA TGGGGAGGAG
    CTTCACGGAG GCAAGAGGGT AATGGAGTGC CTAAAAAAAG CTCTAAAAAT AGCAAATCAG
    TGCATGGACC CCTCTCTACA AGTGCAGCTT TTTATAGAAA TTCTGAACAG ATATATCTAT
    TTTTATGAAA AGGAAAATGA TGCGGTAACA ATTCAGGTTT TAAACCAGCT TATCCAAAAG
    ATTCGAGAAG ACCTCCCGAA TCTTGAATCC AGTGAAGAAA CAGAGCAGAT TAACAAACAT
    TTTCATAACA CACTGGAGCA TTTGCGCTTG CGGCGGGAAT CACCAGAATC CGAGGGGCCA
    ATTTATGAAG GTCTCATCCT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011521529.1
    CDS916..3219
    Translation

    Target ORF information:

    RefSeq Version XM_011523227.3
    Organism Homo sapiens(human)
    Definition Homo sapiens VPS35 retromer complex component (VPS35), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011523227.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAAGAGAT GCCTGGACAA AAACAAGCTT ATGGATGCTC TAAAACATGC TTCTAATATG 
    CTTGGTGAAC TCCGGACTTC TATGTTATCA CCAAAGAGTT ACTATGAACT TTATATGGCC
    ATTTCTGATG AACTGCACTA CTTGGAGGTC TACCTGACAG ATGAGTTTGC TAAAGGAAGG
    AAAGTGGCAG ATCTCTACGA ACTTGTACAG TATGCTGGAA ACATTATCCC AAGGCTTTAC
    CTTTTGATCA CAGTTGGAGT TGTATATGTC AAGTCATTTC CTCAGTCCAG GAAGGATATT
    TTGAAAGATT TGGTAGAAAT GTGCCGTGGT GTGCAACATC CCTTGAGGGG TCTGTTTCTT
    CGAAATTACC TTCTTCAGTG TACCAGAAAT ATCTTACCTG ATGAAGGAGA GCCAACAGAT
    GAAGAAACAA CTGGTGACAT CAGTGATTCC ATGGATTTTG TACTGCTCAA CTTTGCAGAA
    ATGAACAAGC TCTGGGTGCG AATGCAGCAT CAGGGACATA GCCGAGATAG AGAAAAAAGA
    GAACGAGAAA GACAAGAACT GAGAATTTTA GTGGGAACAA ATTTGGTGCG CCTCAGTCAG
    TTGGAAGGTG TAAATGTGGA ACGTTACAAA CAGATTGTTT TGACTGGCAT ATTGGAGCAA
    GTTGTAAACT GTAGGGATGC TTTGGCTCAA GAATATCTCA TGGAGTGTAT TATTCAGGTT
    TTCCCTGATG AATTTCACCT CCAGACTTTG AATCCTTTTC TTCGGGCCTG TGCTGAGTTA
    CACCAGAATG TAAATGTGAA GAACATAATC ATTGCTTTAA TTGATAGATT AGCTTTATTT
    GCTCACCGTG AAGATGGACC TGGAATCCCA GCGGATATTA AACTTTTTGA TATATTTTCA
    CAGCAGGTGG CTACAGTGAT ACAGTCTAGA CAAGACATGC CTTCAGAGGA TGTTGTATCT
    TTACAAGTCT CTCTGATTAA TCTTGCCATG AAATGTTACC CTGATCGTGT GGACTATGTT
    GATAAAGTTC TAGAAACAAC AGTGGAGATA TTCAATAAGC TCAACCTTGA ACATATTGCT
    ACCAGTAGTG CAGTTTCAAA GGAACTCACC AGACTTTTGA AAATACCAGT TGACACTTAC
    AACAATATTT TAACAGTCTT GAAATTAAAA CATTTTCACC CACTCTTTGA GTACTTTGAC
    TACGAGTCCA GAAAGAGCAT GAGTTGTTAT GTGCTTAGTA ATGTTCTGGA TTATAACACA
    GAAATTGTCT CTCAAGACCA GGTGGATTCC ATAATGAATT TGGTATCCAC GTTGATTCAA
    GATCAGCCAG ATCAACCTGT AGAAGACCCT GATCCAGAAG ATTTTGCTGA TGAGCAGAGC
    CTTGTGGGCC GCTTCATTCA TCTGCTGCGC TCTGAGGACC CTGACCAGCA GTACTTGATT
    TTGAACACAG CACGAAAACA TTTTGGAGCT GGTGGAAATC AGCGGATTCG CTTCACACTG
    CCACCTTTGG TATTTGCAGC TTACCAGCTG GCTTTTCGAT ATAAAGAGAA TTCTAAAGTG
    GATGACAAAT GGGAAAAGAA ATGCCAGAAG ATTTTTTCAT TTGCCCACCA GACTATCAGT
    GCTTTGATCA AAGCAGAGCT GGCAGAATTG CCCTTAAGAC TTTTTCTTCA AGGAGCACTA
    GCTGCTGGGG AAATTGGTTT TGAAAATCAT GAGACAGTCG CATATGAATT CATGTCCCAG
    GCATTTTCTC TGTATGAAGA TGAAATCAGC GATTCCAAAG CACAGCTAGC TGCCATCACC
    TTGATCATTG GCACTTTTGA AAGGATGAAG TGCTTCAGTG AAGAGAATCA CGAACCTCTG
    AGGACTCAGT GTGCCCTTGC TGCATCCAAA CTTCTAAAGA AACCTGATCA GGGCCGAGCT
    GTGAGCACCT GTGCACATCT CTTCTGGTCT GGCAGAAACA CGGACAAAAA TGGGGAGGAG
    CTTCACGGAG GCAAGAGGGT AATGGAGTGC CTAAAAAAAG CTCTAAAAAT AGCAAATCAG
    TGCATGGACC CCTCTCTACA AGTGCAGCTT TTTATAGAAA TTCTGAACAG ATATATCTAT
    TTTTATGAAA AGGAAAATGA TGCGGTAACA ATTCAGGTTT TAAACCAGCT TATCCAAAAG
    ATTCGAGAAG ACCTCCCGAA TCTTGAATCC AGTGAAGAAA CAGAGCAGAT TAACAAACAT
    TTTCATAACA CACTGGAGCA TTTGCGCTTG CGGCGGGAAT CACCAGAATC CGAGGGGCCA
    ATTTATGAAG GTCTCATCCT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu108594
    Clone ID Related Accession (Same CDS sequence) NM_018206.6
    Accession Version NM_018206.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2391bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-10
    Organism Homo sapiens(human)
    Product vacuolar protein sorting-associated protein 35
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC007225.2 and AC012186.8. On May 17, 2019 this sequence version replaced NM_018206.5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.283710.1, SRR1803611.161623.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000299138.12/ ENSP00000299138.7 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCTACAA CACAGCAGTC CCCTCAGGAT GAGCAGGAAA AGCTCTTGGA TGAAGCCATA 
    CAGGCTGTGA AGGTCCAGTC ATTCCAAATG AAGAGATGCC TGGACAAAAA CAAGCTTATG
    GATGCTCTAA AACATGCTTC TAATATGCTT GGTGAACTCC GGACTTCTAT GTTATCACCA
    AAGAGTTACT ATGAACTTTA TATGGCCATT TCTGATGAAC TGCACTACTT GGAGGTCTAC
    CTGACAGATG AGTTTGCTAA AGGAAGGAAA GTGGCAGATC TCTACGAACT TGTACAGTAT
    GCTGGAAACA TTATCCCAAG GCTTTACCTT TTGATCACAG TTGGAGTTGT ATATGTCAAG
    TCATTTCCTC AGTCCAGGAA GGATATTTTG AAAGATTTGG TAGAAATGTG CCGTGGTGTG
    CAACATCCCT TGAGGGGTCT GTTTCTTCGA AATTACCTTC TTCAGTGTAC CAGAAATATC
    TTACCTGATG AAGGAGAGCC AACAGATGAA GAAACAACTG GTGACATCAG TGATTCCATG
    GATTTTGTAC TGCTCAACTT TGCAGAAATG AACAAGCTCT GGGTGCGAAT GCAGCATCAG
    GGACATAGCC GAGATAGAGA AAAAAGAGAA CGAGAAAGAC AAGAACTGAG AATTTTAGTG
    GGAACAAATT TGGTGCGCCT CAGTCAGTTG GAAGGTGTAA ATGTGGAACG TTACAAACAG
    ATTGTTTTGA CTGGCATATT GGAGCAAGTT GTAAACTGTA GGGATGCTTT GGCTCAAGAA
    TATCTCATGG AGTGTATTAT TCAGGTTTTC CCTGATGAAT TTCACCTCCA GACTTTGAAT
    CCTTTTCTTC GGGCCTGTGC TGAGTTACAC CAGAATGTAA ATGTGAAGAA CATAATCATT
    GCTTTAATTG ATAGATTAGC TTTATTTGCT CACCGTGAAG ATGGACCTGG AATCCCAGCG
    GATATTAAAC TTTTTGATAT ATTTTCACAG CAGGTGGCTA CAGTGATACA GTCTAGACAA
    GACATGCCTT CAGAGGATGT TGTATCTTTA CAAGTCTCTC TGATTAATCT TGCCATGAAA
    TGTTACCCTG ATCGTGTGGA CTATGTTGAT AAAGTTCTAG AAACAACAGT GGAGATATTC
    AATAAGCTCA ACCTTGAACA TATTGCTACC AGTAGTGCAG TTTCAAAGGA ACTCACCAGA
    CTTTTGAAAA TACCAGTTGA CACTTACAAC AATATTTTAA CAGTCTTGAA ATTAAAACAT
    TTTCACCCAC TCTTTGAGTA CTTTGACTAC GAGTCCAGAA AGAGCATGAG TTGTTATGTG
    CTTAGTAATG TTCTGGATTA TAACACAGAA ATTGTCTCTC AAGACCAGGT GGATTCCATA
    ATGAATTTGG TATCCACGTT GATTCAAGAT CAGCCAGATC AACCTGTAGA AGACCCTGAT
    CCAGAAGATT TTGCTGATGA GCAGAGCCTT GTGGGCCGCT TCATTCATCT GCTGCGCTCT
    GAGGACCCTG ACCAGCAGTA CTTGATTTTG AACACAGCAC GAAAACATTT TGGAGCTGGT
    GGAAATCAGC GGATTCGCTT CACACTGCCA CCTTTGGTAT TTGCAGCTTA CCAGCTGGCT
    TTTCGATATA AAGAGAATTC TAAAGTGGAT GACAAATGGG AAAAGAAATG CCAGAAGATT
    TTTTCATTTG CCCACCAGAC TATCAGTGCT TTGATCAAAG CAGAGCTGGC AGAATTGCCC
    TTAAGACTTT TTCTTCAAGG AGCACTAGCT GCTGGGGAAA TTGGTTTTGA AAATCATGAG
    ACAGTCGCAT ATGAATTCAT GTCCCAGGCA TTTTCTCTGT ATGAAGATGA AATCAGCGAT
    TCCAAAGCAC AGCTAGCTGC CATCACCTTG ATCATTGGCA CTTTTGAAAG GATGAAGTGC
    TTCAGTGAAG AGAATCACGA ACCTCTGAGG ACTCAGTGTG CCCTTGCTGC ATCCAAACTT
    CTAAAGAAAC CTGATCAGGG CCGAGCTGTG AGCACCTGTG CACATCTCTT CTGGTCTGGC
    AGAAACACGG ACAAAAATGG GGAGGAGCTT CACGGAGGCA AGAGGGTAAT GGAGTGCCTA
    AAAAAAGCTC TAAAAATAGC AAATCAGTGC ATGGACCCCT CTCTACAAGT GCAGCTTTTT
    ATAGAAATTC TGAACAGATA TATCTATTTT TATGAAAAGG AAAATGATGC GGTAACAATT
    CAGGTTTTAA ACCAGCTTAT CCAAAAGATT CGAGAAGACC TCCCGAATCT TGAATCCAGT
    GAAGAAACAG AGCAGATTAA CAAACATTTT CATAACACAC TGGAGCATTT GCGCTTGCGG
    CGGGAATCAC CAGAATCCGA GGGGCCAATT TATGAAGGTC TCATCCTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_060676.2
    CDS46..2436
    Translation

    Target ORF information:

    RefSeq Version NM_018206.6
    Organism Homo sapiens(human)
    Definition Homo sapiens VPS35 retromer complex component (VPS35), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_018206.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCTACAA CACAGCAGTC CCCTCAGGAT GAGCAGGAAA AGCTCTTGGA TGAAGCCATA 
    CAGGCTGTGA AGGTCCAGTC ATTCCAAATG AAGAGATGCC TGGACAAAAA CAAGCTTATG
    GATGCTCTAA AACATGCTTC TAATATGCTT GGTGAACTCC GGACTTCTAT GTTATCACCA
    AAGAGTTACT ATGAACTTTA TATGGCCATT TCTGATGAAC TGCACTACTT GGAGGTCTAC
    CTGACAGATG AGTTTGCTAA AGGAAGGAAA GTGGCAGATC TCTACGAACT TGTACAGTAT
    GCTGGAAACA TTATCCCAAG GCTTTACCTT TTGATCACAG TTGGAGTTGT ATATGTCAAG
    TCATTTCCTC AGTCCAGGAA GGATATTTTG AAAGATTTGG TAGAAATGTG CCGTGGTGTG
    CAACATCCCT TGAGGGGTCT GTTTCTTCGA AATTACCTTC TTCAGTGTAC CAGAAATATC
    TTACCTGATG AAGGAGAGCC AACAGATGAA GAAACAACTG GTGACATCAG TGATTCCATG
    GATTTTGTAC TGCTCAACTT TGCAGAAATG AACAAGCTCT GGGTGCGAAT GCAGCATCAG
    GGACATAGCC GAGATAGAGA AAAAAGAGAA CGAGAAAGAC AAGAACTGAG AATTTTAGTG
    GGAACAAATT TGGTGCGCCT CAGTCAGTTG GAAGGTGTAA ATGTGGAACG TTACAAACAG
    ATTGTTTTGA CTGGCATATT GGAGCAAGTT GTAAACTGTA GGGATGCTTT GGCTCAAGAA
    TATCTCATGG AGTGTATTAT TCAGGTTTTC CCTGATGAAT TTCACCTCCA GACTTTGAAT
    CCTTTTCTTC GGGCCTGTGC TGAGTTACAC CAGAATGTAA ATGTGAAGAA CATAATCATT
    GCTTTAATTG ATAGATTAGC TTTATTTGCT CACCGTGAAG ATGGACCTGG AATCCCAGCG
    GATATTAAAC TTTTTGATAT ATTTTCACAG CAGGTGGCTA CAGTGATACA GTCTAGACAA
    GACATGCCTT CAGAGGATGT TGTATCTTTA CAAGTCTCTC TGATTAATCT TGCCATGAAA
    TGTTACCCTG ATCGTGTGGA CTATGTTGAT AAAGTTCTAG AAACAACAGT GGAGATATTC
    AATAAGCTCA ACCTTGAACA TATTGCTACC AGTAGTGCAG TTTCAAAGGA ACTCACCAGA
    CTTTTGAAAA TACCAGTTGA CACTTACAAC AATATTTTAA CAGTCTTGAA ATTAAAACAT
    TTTCACCCAC TCTTTGAGTA CTTTGACTAC GAGTCCAGAA AGAGCATGAG TTGTTATGTG
    CTTAGTAATG TTCTGGATTA TAACACAGAA ATTGTCTCTC AAGACCAGGT GGATTCCATA
    ATGAATTTGG TATCCACGTT GATTCAAGAT CAGCCAGATC AACCTGTAGA AGACCCTGAT
    CCAGAAGATT TTGCTGATGA GCAGAGCCTT GTGGGCCGCT TCATTCATCT GCTGCGCTCT
    GAGGACCCTG ACCAGCAGTA CTTGATTTTG AACACAGCAC GAAAACATTT TGGAGCTGGT
    GGAAATCAGC GGATTCGCTT CACACTGCCA CCTTTGGTAT TTGCAGCTTA CCAGCTGGCT
    TTTCGATATA AAGAGAATTC TAAAGTGGAT GACAAATGGG AAAAGAAATG CCAGAAGATT
    TTTTCATTTG CCCACCAGAC TATCAGTGCT TTGATCAAAG CAGAGCTGGC AGAATTGCCC
    TTAAGACTTT TTCTTCAAGG AGCACTAGCT GCTGGGGAAA TTGGTTTTGA AAATCATGAG
    ACAGTCGCAT ATGAATTCAT GTCCCAGGCA TTTTCTCTGT ATGAAGATGA AATCAGCGAT
    TCCAAAGCAC AGCTAGCTGC CATCACCTTG ATCATTGGCA CTTTTGAAAG GATGAAGTGC
    TTCAGTGAAG AGAATCACGA ACCTCTGAGG ACTCAGTGTG CCCTTGCTGC ATCCAAACTT
    CTAAAGAAAC CTGATCAGGG CCGAGCTGTG AGCACCTGTG CACATCTCTT CTGGTCTGGC
    AGAAACACGG ACAAAAATGG GGAGGAGCTT CACGGAGGCA AGAGGGTAAT GGAGTGCCTA
    AAAAAAGCTC TAAAAATAGC AAATCAGTGC ATGGACCCCT CTCTACAAGT GCAGCTTTTT
    ATAGAAATTC TGAACAGATA TATCTATTTT TATGAAAAGG AAAATGATGC GGTAACAATT
    CAGGTTTTAA ACCAGCTTAT CCAAAAGATT CGAGAAGACC TCCCGAATCT TGAATCCAGT
    GAAGAAACAG AGCAGATTAA CAAACATTTT CATAACACAC TGGAGCATTT GCGCTTGCGG
    CGGGAATCAC CAGAATCCGA GGGGCCAATT TATGAAGGTC TCATCCTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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