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MBD5 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol MBD5
Entrez Gene ID 55777
Full Name methyl-CpG binding domain protein 5
Synonyms MRD1
General protein information
Preferred Names

methyl-CpG binding domain protein 5

Names

methyl-CpG-binding domain protein 5
methyl-CpG-binding protein MBD5
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

2

2q23.1
Summary This gene encodes a member of the methyl-CpG-binding domain (MBD) family. The MBD consists of about 70 residues and is the minimal region required for a methyl-CpG-binding protein binding specifically to methylated DNA. In addition to the MBD domain, this protein contains a PWWP domain (Pro-Trp-Trp-Pro motif), which consists of 100-150 amino acids and is found in numerous proteins that are involved in cell division, growth and differentiation. Mutations in this gene cause an autosomal dominant type of cognitive disability. The encoded protein interacts with the polycomb repressive complex PR-DUB which catalyzes the deubiquitination of a lysine residue of histone 2A. Haploinsufficiency of this gene is associated with a syndrome involving microcephaly, intellectual disabilities, severe speech impairment, and seizures. Alternatively spliced transcript variants have been found, but their full-length nature is not determined. [provided by RefSeq, Jul 2017].
Disorder MIM:

611472

Disorder Html: Mental retardation, autosomal dominant 1, 156200 (3)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_017004482.1 XP_016859971.1 methyl-CpG-binding domain protein 5 isoform X2
XM_017004484.1 XP_016859973.1 methyl-CpG-binding domain protein 5 isoform X4
XM_017004483.1 XP_016859972.1 methyl-CpG-binding domain protein 5 isoform X3
NM_018328.4 NP_060798.2 methyl-CpG-binding domain protein 5
XM_011511470.2 XP_011509772.1 methyl-CpG-binding domain protein 5 isoform X1
XM_017004485.1 XP_016859974.1 methyl-CpG-binding domain protein 5 isoform X5
XM_011511472.2 XP_011509774.1 methyl-CpG-binding domain protein 5 isoform X1

Pathways from BioSystems


Homologies


Homo sapiens (human) MBD5 NP_060798.2
Pan troglodytes (chimpanzee) MBD5 XP_003309303.1
Mus musculus (house mouse) Mbd5 NP_084200.1
Xenopus tropicalis (tropical clawed frog) mbd5 XP_002943521.2
MBD5 XP_002798916.1
Bos taurus (cattle) MBD5 NP_001178197.1
Gallus gallus (chicken) MBD5 XP_004943074.1
Canis lupus familiaris (dog) MBD5 XP_541030.2

Gene Ontology


Bibliography

Related articles in PubMed

Diagnostic exome sequencing identifies a heterozygous MBD5 frameshift mutation in a family with intellectual disability and epilepsy.
Han JY, Lee IG, Jang W, Kim M, Kim Y, Jang JH, Park J
European journal of medical genetics60(10)559-564(2017 Oct)

MBD5 haploinsufficiency is associated with sleep disturbance and disrupts circadian pathways common to Smith-Magenis and fragile X syndromes.
Mullegama SV, Pugliesi L, Burns B, Shah Z, Tahir R, Gu Y, Nelson DL, Elsea SH
European journal of human genetics : EJHG23(6)781-9(2015 Jun)

A molecular model for neurodevelopmental disorders.
Gigek CO, Chen ES, Ota VK, Maussion G, Peng H, Vaillancourt K, Diallo AB, Lopez JP, Crapper L, Vasuta C, Chen GG, Ernst C
Translational psychiatry5e565(2015 May)

Phenotypic and molecular convergence of 2q23.1 deletion syndrome with other neurodevelopmental syndromes associated with autism spectrum disorder.
Mullegama SV, Alaimo JT, Chen L, Elsea SH
International journal of molecular sciences16(4)7627-43(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A novel frameshift mutation c.254_255delGA (p.Arg85Asnfs*6) in the MBD5 gene was identified in a family with intellectual disability and epilepsy.
Title: Diagnostic exome sequencing identifies a heterozygous MBD5 frameshift mutation in a family with intellectual disability and epilepsy.

Circadian rhythm gene expression altered by haploinsufficiency of MBD5.
Title: MBD5 haploinsufficiency is associated with sleep disturbance and disrupts circadian pathways common to Smith-Magenis and fragile X syndromes.

Reduced MBD5 dosage leads to mRNA and microRNA expression patterns and DNA methylation patterns more characteristic of differentiating than proliferating neural stem cells. This balance change may underlie neurodevelopmental disorders.
Title: A molecular model for neurodevelopmental disorders.

Results show that when MBD5 and RAI1 are haploinsufficient, they perturb several common pathways that are linked to neuronal and behavioral development.
Title: Phenotypic and molecular convergence of 2q23.1 deletion syndrome with other neurodevelopmental syndromes associated with autism spectrum disorder.

A genomic copy number variant analysis implicates the MBD5 and HNRNPU genes in Chinese children with infantile spasms and expands the clinical spectrum of 2q23.1 deletion.
Title: A genomic copy number variant analysis implicates the MBD5 and HNRNPU genes in Chinese children with infantile spasms and expands the clinical spectrum of 2q23.1 deletion.

The following MBD5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MBD5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu19437 NM_018328.4
Latest version!		 Homo sapiens methyl-CpG binding domain protein 5 (MBD5), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu36432 XM_017004482.1
Latest version!		 Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $951.30
$1359.00
OHu69742 XM_011511470.2
Latest version!		 Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $951.30
$1359.00
OHu69742 XM_011511472.2
Latest version!		 Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $951.30
$1359.00
OHu69743 XM_017004483.1
Latest version!		 Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $951.30
$1359.00
OHu69744 XM_017004484.1
Latest version!		 Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $951.30
$1359.00
OHu69745 XM_017004485.1
Latest version!		 Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $811.30
$1159.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu19437
Clone ID Related Accession (Same CDS sequence) NM_018328.4
Accession Version NM_018328.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 4485bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-12-11
Organism Homo sapiens(human)
Product methyl-CpG-binding domain protein 5
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA240785.1, EF542797.1, AB040894.1 and CA449443.1. This sequence is a reference standard in the RefSeqGene project. On or before Aug 31, 2013 this sequence version replaced XM_005263713.1, NM_018328.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: EF542797.1, BC150264.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGTGAT ATGTCATCAA TAAACAATAC TTTGAGTAAC 
CATCAACTGA CTCATCTACA GTCGCTGTTA AACAACAATC AGATGTTTCC TCCAAATCAG 
CAACAGCAGC AACTTCTCCA GGGGTACCAG AATCTCCAGG CGTTCCAAGG ACAGTCCACA 
ATTCCTTGCC CAGCTAACAA TAACCCCATG GCTTGTCTGT TTCAGAACTT TCAGGTGAGA 
ATGCAGGAAG ATGCAGCTCT CCTAAACAAA AGAATAAGCA CTCAGCCTGG GCTCACAGCA 
CTTCCTGAGA ATCCAAACAC TACACTTCCA CCTTTTCAAG ATACACCTTG TGAGTTGCAA 
CCGAGGATTG ACCCATCTCT TGGTCAACAG GTGAAGGATG GCCTCGTTGT GGGTGGCCCA 
GGTGATGCTT CCGTAGATGC CATTTACAAA GCAGTTGTCG ATGCAGCCAG CAAAGGAATG 
CAGGTTGTCA TCACCACTGC AGTCAACAGT ACAACTCAGA TCAGCCCCAT TCCAGCTCTG 
AGTGCCATGA GTGCCTTCAC TGCCTCAATT GGTGACCCAT TAAATCTCTC CAGTGCTGTC 
AGTGCGGTCA TTCATGGACG GAACATGGGA GGTGTTGATC ATGATGGTAG GCTGAGGAAT 
TCAAGAGGGG CTCGGCTGCC CAAGAATCTA GACCATGGGA AAAATGTGAA CGAAGGAGAT 
GGGTTTGAAT ATTTCAAGTC AGCAAGTTGC CACACATCCA AAAAACAGTG GGACGGGGAG 
CAAAGCCCCA GAGGGGAGCG AAACAGGTGG AAGTACGAGG AATTTTTAGA TCATCCAGGC 
CATATCCACA GTAGTCCTTG TCATGAAAGG CCCAACAATG TCTCTACACT GCCATTTCTG 
CCTGGGGAAC AGCACCCAAT ACTGTTACCA CCAAGAAACT GTCCAGGGGA TAAAATTCTA 
GAGGAAAATT TCAGGTATAA TAACTACAAA AGAACTATGA TGAGTTTTAA GGAGAGACTA 
GAGAACACTG TGGAAAGATG TGCACACATA AATGGGAATA GACCTCGACA GAGTCGGGGA 
TTTGGAGAGC TGCTAAGCAC TGCAAAGCAA GACCTGGTCC TAGAGGAGCA GTCTCCAAGT 
TCCTCAAATA GTTTGGAAAA TTCTCTGGTC AAAGACTACA TCCATTACAA TGGAGACTTT 
AATGCCAAAA GCGTTAATGG GTGTGTGCCT AGCCCTTCAG ATGCTAAAAG CATTAGTAGT 
GAAGATGACC TAAGGAACCC AGACTCCCCC TCTTCAAATG AATTGATACA TTATAGACCA 
AGGACGTTCA ATGTTGGCGA CTTGGTCTGG GGCCAAATCA AAGGACTGAC TTCCTGGCCT 
GGAAAATTAG TAAGAGAAGA CGACGTTCAC AATTCATGTC AACAAAGCCC CGAGGAAGGG 
AAGGTGGAGC CCGAGAAGTT GAAGACACTA ACAGAAGGTT TGGAAGCCTA CAGCCGTGTC 
CGGAAAAGGA ACAGAAAAAG TGGAAAGCTA AATAACCATT TAGAAGCTGC TATTCATGAG 
GCCATGAGTG AACTGGACAA AATGTCTGGG ACTGTACACC AAATCCCACA GGGTGACAGA 
CAAATGAGAC CCCCCAAACC CAAGAGGAGG AAGATCTCCA GATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_060798.2
CDS998..5482
Misc Feature(1)983..985
Misc Feature(2)1058..1234
Misc Feature(3)5144..5377
Misc Feature(4)order(5174..5176,5183..5185,5192..5194,5228..5230,5243..5245,5249..5251)
Exon (1)1..36
Exon (2)37..73
Exon (3)74..167
Exon (4)168..318
Exon (5)319..441
Exon (6)442..1110
Exon (7)1111..1213
Exon (8)1214..1394
Exon (9)1395..3515
Exon (10)3516..3842
Exon (11)3843..4051
Exon (12)4052..5260
Exon (13)5261..5334
Exon (14)5335..5410
Exon (15)5411..6018
Translation

Target ORF information:

RefSeq Version NM_018328.4
Organism Homo sapiens(human)
Definition Homo sapiens methyl-CpG binding domain protein 5 (MBD5), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_018328.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGTGAT ATGTCATCAA TAAACAATAC TTTGAGTAAC 
CATCAACTGA CTCATCTACA GTCGCTGTTA AACAACAATC AGATGTTTCC TCCAAATCAG 
CAACAGCAGC AACTTCTCCA GGGGTACCAG AATCTCCAGG CGTTCCAAGG ACAGTCCACA 
ATTCCTTGCC CAGCTAACAA TAACCCCATG GCTTGTCTGT TTCAGAACTT TCAGGTGAGA 
ATGCAGGAAG ATGCAGCTCT CCTAAACAAA AGAATAAGCA CTCAGCCTGG GCTCACAGCA 
CTTCCTGAGA ATCCAAACAC TACACTTCCA CCTTTTCAAG ATACACCTTG TGAGTTGCAA 
CCGAGGATTG ACCCATCTCT TGGTCAACAG GTGAAGGATG GCCTCGTTGT GGGTGGCCCA 
GGTGATGCTT CCGTAGATGC CATTTACAAA GCAGTTGTCG ATGCAGCCAG CAAAGGAATG 
CAGGTTGTCA TCACCACTGC AGTCAACAGT ACAACTCAGA TCAGCCCCAT TCCAGCTCTG 
AGTGCCATGA GTGCCTTCAC TGCCTCAATT GGTGACCCAT TAAATCTCTC CAGTGCTGTC 
AGTGCGGTCA TTCATGGACG GAACATGGGA GGTGTTGATC ATGATGGTAG GCTGAGGAAT 
TCAAGAGGGG CTCGGCTGCC CAAGAATCTA GACCATGGGA AAAATGTGAA CGAAGGAGAT 
GGGTTTGAAT ATTTCAAGTC AGCAAGTTGC CACACATCCA AAAAACAGTG GGACGGGGAG 
CAAAGCCCCA GAGGGGAGCG AAACAGGTGG AAGTACGAGG AATTTTTAGA TCATCCAGGC 
CATATCCACA GTAGTCCTTG TCATGAAAGG CCCAACAATG TCTCTACACT GCCATTTCTG 
CCTGGGGAAC AGCACCCAAT ACTGTTACCA CCAAGAAACT GTCCAGGGGA TAAAATTCTA 
GAGGAAAATT TCAGGTATAA TAACTACAAA AGAACTATGA TGAGTTTTAA GGAGAGACTA 
GAGAACACTG TGGAAAGATG TGCACACATA AATGGGAATA GACCTCGACA GAGTCGGGGA 
TTTGGAGAGC TGCTAAGCAC TGCAAAGCAA GACCTGGTCC TAGAGGAGCA GTCTCCAAGT 
TCCTCAAATA GTTTGGAAAA TTCTCTGGTC AAAGACTACA TCCATTACAA TGGAGACTTT 
AATGCCAAAA GCGTTAATGG GTGTGTGCCT AGCCCTTCAG ATGCTAAAAG CATTAGTAGT 
GAAGATGACC TAAGGAACCC AGACTCCCCC TCTTCAAATG AATTGATACA TTATAGACCA 
AGGACGTTCA ATGTTGGCGA CTTGGTCTGG GGCCAAATCA AAGGACTGAC TTCCTGGCCT 
GGAAAATTAG TAAGAGAAGA CGACGTTCAC AATTCATGTC AACAAAGCCC CGAGGAAGGG 
AAGGTGGAGC CCGAGAAGTT GAAGACACTA ACAGAAGGTT TGGAAGCCTA CAGCCGTGTC 
CGGAAAAGGA ACAGAAAAAG TGGAAAGCTA AATAACCATT TAGAAGCTGC TATTCATGAG 
GCCATGAGTG AACTGGACAA AATGTCTGGG ACTGTACACC AAATCCCACA GGGTGACAGA 
CAAATGAGAC CCCCCAAACC CAAGAGGAGG AAGATCTCCA GATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu36432
Clone ID Related Accession (Same CDS sequence) XM_017004482.1
Accession Version XM_017004482.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 5184bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product methyl-CpG-binding domain protein 5 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT 
CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA 
TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT 
GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT 
ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG 
TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC 
TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA 
GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC 
ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC 
ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG 
TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT 
AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA 
CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG 
TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG 
GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT 
AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC 
CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT 
ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT 
GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC 
ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA 
GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT 
GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG 
AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC 
AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA 
GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA 
AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT 
CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA 
CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT 
AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT 
GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT 
GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT 
TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG 
TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA 
GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC 
TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC 
GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTGGAGCC CGAGAAGTTG 
AAGACACTAA CAGAAGGTTT GGAAGCCTAC AGCCGTGTCC GGAAAAGGAA CAGAAAAAGT 
GGAAAGCTAA ATAACCATTT AGAAGCTGCT ATTCATGAGG CCATGAGTGA ACTGGACAAA 
ATGTCTGGGA CTGTACACCA AATCCCACAG GGTGACAGAC AAATGAGACC CCCCAAACCC 
AAGAGGAGGA AGATCTCCAG ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016859971.1
CDS680..5863
Misc Feature(1)740..916
Misc Feature(2)5525..5758
Misc Feature(3)order(5555..5557,5564..5566,5573..5575,5609..5611,5624..5626,5630..5632)
Translation

Target ORF information:

RefSeq Version XM_017004482.1
Organism Homo sapiens(human)
Definition Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017004482.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT 
CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA 
TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT 
GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT 
ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG 
TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC 
TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA 
GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC 
ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC 
ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG 
TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT 
AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA 
CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG 
TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG 
GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT 
AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC 
CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT 
ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT 
GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC 
ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA 
GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT 
GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG 
AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC 
AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA 
GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA 
AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT 
CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA 
CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT 
AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT 
GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT 
GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT 
TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG 
TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA 
GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC 
TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC 
GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTGGAGCC CGAGAAGTTG 
AAGACACTAA CAGAAGGTTT GGAAGCCTAC AGCCGTGTCC GGAAAAGGAA CAGAAAAAGT 
GGAAAGCTAA ATAACCATTT AGAAGCTGCT ATTCATGAGG CCATGAGTGA ACTGGACAAA 
ATGTCTGGGA CTGTACACCA AATCCCACAG GGTGACAGAC AAATGAGACC CCCCAAACCC 
AAGAGGAGGA AGATCTCCAG ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu69742
Clone ID Related Accession (Same CDS sequence) XM_011511470.2 , XM_011511472.2
Accession Version XM_011511470.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 5223bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product methyl-CpG-binding domain protein 5 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511470.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT 
CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA 
TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT 
GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT 
ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG 
TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC 
TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA 
GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC 
ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC 
ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG 
TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT 
AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA 
CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG 
TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG 
GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT 
AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC 
CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT 
ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT 
GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC 
ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA 
GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT 
GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG 
AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC 
AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA 
GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA 
AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT 
CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA 
CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT 
AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT 
GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT 
GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT 
TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG 
TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA 
GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC 
TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC 
GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTCTGGGT AATGTGGTTT 
GGTCTTCATA CCTTCACTCA GGTGGAGCCC GAGAAGTTGA AGACACTAAC AGAAGGTTTG 
GAAGCCTACA GCCGTGTCCG GAAAAGGAAC AGAAAAAGTG GAAAGCTAAA TAACCATTTA 
GAAGCTGCTA TTCATGAGGC CATGAGTGAA CTGGACAAAA TGTCTGGGAC TGTACACCAA 
ATCCCACAGG GTGACAGACA AATGAGACCC CCCAAACCCA AGAGGAGGAA GATCTCCAGA 
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011509772.1
CDS1230..6452
Misc Feature(1)1290..1466
Misc Feature(2)6075..6347
Misc Feature(3)order(6105..6107,6114..6116,6123..6125,6204..6206,6213..6215,6219..6221)
Translation

Target ORF information:

RefSeq Version XM_011511470.2
Organism Homo sapiens(human)
Definition Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011511470.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT 
CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA 
TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT 
GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT 
ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG 
TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC 
TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA 
GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC 
ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC 
ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG 
TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT 
AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA 
CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG 
TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG 
GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT 
AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC 
CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT 
ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT 
GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC 
ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA 
GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT 
GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG 
AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC 
AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA 
GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA 
AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT 
CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA 
CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT 
AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT 
GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT 
GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT 
TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG 
TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA 
GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC 
TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC 
GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTCTGGGT AATGTGGTTT 
GGTCTTCATA CCTTCACTCA GGTGGAGCCC GAGAAGTTGA AGACACTAAC AGAAGGTTTG 
GAAGCCTACA GCCGTGTCCG GAAAAGGAAC AGAAAAAGTG GAAAGCTAAA TAACCATTTA 
GAAGCTGCTA TTCATGAGGC CATGAGTGAA CTGGACAAAA TGTCTGGGAC TGTACACCAA 
ATCCCACAGG GTGACAGACA AATGAGACCC CCCAAACCCA AGAGGAGGAA GATCTCCAGA 
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu69742
Clone ID Related Accession (Same CDS sequence) XM_011511470.2 , XM_011511472.2
Accession Version XM_011511472.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 5223bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product methyl-CpG-binding domain protein 5 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511472.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT 
CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA 
TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT 
GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT 
ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG 
TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC 
TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA 
GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC 
ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC 
ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG 
TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT 
AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA 
CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG 
TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG 
GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT 
AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC 
CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT 
ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT 
GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC 
ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA 
GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT 
GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG 
AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC 
AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA 
GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA 
AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT 
CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA 
CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT 
AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT 
GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT 
GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT 
TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG 
TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA 
GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC 
TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC 
GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTCTGGGT AATGTGGTTT 
GGTCTTCATA CCTTCACTCA GGTGGAGCCC GAGAAGTTGA AGACACTAAC AGAAGGTTTG 
GAAGCCTACA GCCGTGTCCG GAAAAGGAAC AGAAAAAGTG GAAAGCTAAA TAACCATTTA 
GAAGCTGCTA TTCATGAGGC CATGAGTGAA CTGGACAAAA TGTCTGGGAC TGTACACCAA 
ATCCCACAGG GTGACAGACA AATGAGACCC CCCAAACCCA AGAGGAGGAA GATCTCCAGA 
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011509774.1
CDS810..6032
Misc Feature(1)870..1046
Misc Feature(2)5655..5927
Misc Feature(3)order(5685..5687,5694..5696,5703..5705,5784..5786,5793..5795,5799..5801)
Translation

Target ORF information:

RefSeq Version XM_011511472.2
Organism Homo sapiens(human)
Definition Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011511472.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT 
CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA 
TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT 
GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT 
ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG 
TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC 
TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA 
GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC 
ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC 
ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG 
TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT 
AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA 
CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG 
TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG 
GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT 
AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC 
CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT 
ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT 
GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC 
ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA 
GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT 
GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG 
AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC 
AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA 
GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA 
AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT 
CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA 
CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT 
AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT 
GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT 
GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT 
TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG 
TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA 
GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC 
TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC 
GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTCTGGGT AATGTGGTTT 
GGTCTTCATA CCTTCACTCA GGTGGAGCCC GAGAAGTTGA AGACACTAAC AGAAGGTTTG 
GAAGCCTACA GCCGTGTCCG GAAAAGGAAC AGAAAAAGTG GAAAGCTAAA TAACCATTTA 
GAAGCTGCTA TTCATGAGGC CATGAGTGAA CTGGACAAAA TGTCTGGGAC TGTACACCAA 
ATCCCACAGG GTGACAGACA AATGAGACCC CCCAAACCCA AGAGGAGGAA GATCTCCAGA 
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu69743
Clone ID Related Accession (Same CDS sequence) XM_017004483.1
Accession Version XM_017004483.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 5142bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product methyl-CpG-binding domain protein 5 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT 
CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA 
TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT 
GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT 
ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG 
TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC 
TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA 
GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC 
ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC 
ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG 
TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT 
AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA 
CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG 
TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG 
GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT 
AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC 
CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT 
ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT 
GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC 
ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA 
GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT 
GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG 
AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC 
AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA 
GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA 
AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT 
CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA 
CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT 
AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT 
GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT 
GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT 
TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG 
TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA 
GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC 
TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC 
GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTCTGGGT AATGTGGTTT 
GGTCTTCATA CCTTCACTCA GGTGGAGCCC GAGAAGTTGA AGACACTAAC AGAAGGTTTG 
GAAGCCTACA GCCGTGTCCG GAAAAGGAAC AGAAAACTGA GGGGAAGAAG AAAAGAGGAA 
AAGGCTGAAA AAGGAAGGTG TCATTTGGAG CAAGATAATT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016859972.1
CDS680..5821
Misc Feature(1)740..916
Misc Feature(2)5525..5755
Misc Feature(3)order(5555..5557,5564..5566,5573..5575,5654..5656,5663..5665,5669..5671)
Translation

Target ORF information:

RefSeq Version XM_017004483.1
Organism Homo sapiens(human)
Definition Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017004483.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT 
CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA 
TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT 
GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT 
ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG 
TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC 
TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA 
GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC 
ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC 
ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG 
TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT 
AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA 
CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG 
TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG 
GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT 
AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC 
CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT 
ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT 
GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC 
ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA 
GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT 
GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG 
AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC 
AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA 
GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA 
AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT 
CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA 
CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT 
AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT 
GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT 
GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT 
TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG 
TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA 
GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC 
TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC 
GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTCTGGGT AATGTGGTTT 
GGTCTTCATA CCTTCACTCA GGTGGAGCCC GAGAAGTTGA AGACACTAAC AGAAGGTTTG 
GAAGCCTACA GCCGTGTCCG GAAAAGGAAC AGAAAACTGA GGGGAAGAAG AAAAGAGGAA 
AAGGCTGAAA AAGGAAGGTG TCATTTGGAG CAAGATAATT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu69744
Clone ID Related Accession (Same CDS sequence) XM_017004484.1
Accession Version XM_017004484.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 5103bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product methyl-CpG-binding domain protein 5 isoform X4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT 
CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA 
TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT 
GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT 
ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG 
TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC 
TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA 
GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC 
ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC 
ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG 
TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT 
AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA 
CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG 
TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG 
GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT 
AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC 
CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT 
ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT 
GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC 
ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA 
GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT 
GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG 
AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC 
AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA 
GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA 
AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT 
CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA 
CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT 
AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT 
GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT 
GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT 
TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG 
TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA 
GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC 
TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC 
GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTGGAGCC CGAGAAGTTG 
AAGACACTAA CAGAAGGTTT GGAAGCCTAC AGCCGTGTCC GGAAAAGGAA CAGAAAACTG 
AGGGGAAGAA GAAAAGAGGA AAAGGCTGAA AAAGGAAGGT GTCATTTGGA GCAAGATAAT 
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016859973.1
CDS680..5782
Misc Feature(1)740..916
Misc Feature(2)5525..5716
Misc Feature(3)order(5555..5557,5564..5566,5573..5575,5609..5611,5624..5626,5630..5632)
Translation

Target ORF information:

RefSeq Version XM_017004484.1
Organism Homo sapiens(human)
Definition Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017004484.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT 
CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA 
TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT 
GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT 
ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG 
TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC 
TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA 
GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC 
ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC 
ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG 
TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT 
AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA 
CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG 
TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG 
GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT 
AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC 
CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT 
ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT 
GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC 
ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA 
GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT 
GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG 
AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC 
AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA 
GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA 
AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT 
CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA 
CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT 
AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT 
GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT 
GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT 
TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG 
TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA 
GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC 
TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC 
GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTGGAGCC CGAGAAGTTG 
AAGACACTAA CAGAAGGTTT GGAAGCCTAC AGCCGTGTCC GGAAAAGGAA CAGAAAACTG 
AGGGGAAGAA GAAAAGAGGA AAAGGCTGAA AAAGGAAGGT GTCATTTGGA GCAAGATAAT 
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu69745
Clone ID Related Accession (Same CDS sequence) XM_017004485.1
Accession Version XM_017004485.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 4524bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product methyl-CpG-binding domain protein 5 isoform X5
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGTGAT ATGTCATCAA TAAACAATAC TTTGAGTAAC 
CATCAACTGA CTCATCTACA GTCGCTGTTA AACAACAATC AGATGTTTCC TCCAAATCAG 
CAACAGCAGC AACTTCTCCA GGGGTACCAG AATCTCCAGG CGTTCCAAGG ACAGTCCACA 
ATTCCTTGCC CAGCTAACAA TAACCCCATG GCTTGTCTGT TTCAGAACTT TCAGGTGAGA 
ATGCAGGAAG ATGCAGCTCT CCTAAACAAA AGAATAAGCA CTCAGCCTGG GCTCACAGCA 
CTTCCTGAGA ATCCAAACAC TACACTTCCA CCTTTTCAAG ATACACCTTG TGAGTTGCAA 
CCGAGGATTG ACCCATCTCT TGGTCAACAG GTGAAGGATG GCCTCGTTGT GGGTGGCCCA 
GGTGATGCTT CCGTAGATGC CATTTACAAA GCAGTTGTCG ATGCAGCCAG CAAAGGAATG 
CAGGTTGTCA TCACCACTGC AGTCAACAGT ACAACTCAGA TCAGCCCCAT TCCAGCTCTG 
AGTGCCATGA GTGCCTTCAC TGCCTCAATT GGTGACCCAT TAAATCTCTC CAGTGCTGTC 
AGTGCGGTCA TTCATGGACG GAACATGGGA GGTGTTGATC ATGATGGTAG GCTGAGGAAT 
TCAAGAGGGG CTCGGCTGCC CAAGAATCTA GACCATGGGA AAAATGTGAA CGAAGGAGAT 
GGGTTTGAAT ATTTCAAGTC AGCAAGTTGC CACACATCCA AAAAACAGTG GGACGGGGAG 
CAAAGCCCCA GAGGGGAGCG AAACAGGTGG AAGTACGAGG AATTTTTAGA TCATCCAGGC 
CATATCCACA GTAGTCCTTG TCATGAAAGG CCCAACAATG TCTCTACACT GCCATTTCTG 
CCTGGGGAAC AGCACCCAAT ACTGTTACCA CCAAGAAACT GTCCAGGGGA TAAAATTCTA 
GAGGAAAATT TCAGGTATAA TAACTACAAA AGAACTATGA TGAGTTTTAA GGAGAGACTA 
GAGAACACTG TGGAAAGATG TGCACACATA AATGGGAATA GACCTCGACA GAGTCGGGGA 
TTTGGAGAGC TGCTAAGCAC TGCAAAGCAA GACCTGGTCC TAGAGGAGCA GTCTCCAAGT 
TCCTCAAATA GTTTGGAAAA TTCTCTGGTC AAAGACTACA TCCATTACAA TGGAGACTTT 
AATGCCAAAA GCGTTAATGG GTGTGTGCCT AGCCCTTCAG ATGCTAAAAG CATTAGTAGT 
GAAGATGACC TAAGGAACCC AGACTCCCCC TCTTCAAATG AATTGATACA TTATAGACCA 
AGGACGTTCA ATGTTGGCGA CTTGGTCTGG GGCCAAATCA AAGGACTGAC TTCCTGGCCT 
GGAAAATTAG TAAGAGAAGA CGACGTTCAC AATTCATGTC AACAAAGCCC CGAGGAAGGG 
AAGGTCTGGG TAATGTGGTT TGGTCTTCAT ACCTTCACTC AGGTGGAGCC CGAGAAGTTG 
AAGACACTAA CAGAAGGTTT GGAAGCCTAC AGCCGTGTCC GGAAAAGGAA CAGAAAAAGT 
GGAAAGCTAA ATAACCATTT AGAAGCTGCT ATTCATGAGG CCATGAGTGA ACTGGACAAA 
ATGTCTGGGA CTGTACACCA AATCCCACAG GGTGACAGAC AAATGAGACC CCCCAAACCC 
AAGAGGAGGA AGATCTCCAG ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016859974.1
CDS680..5203
Misc Feature(1)740..916
Misc Feature(2)4826..5098
Misc Feature(3)order(4856..4858,4865..4867,4874..4876,4955..4957,4964..4966,4970..4972)
Translation

Target ORF information:

RefSeq Version XM_017004485.1
Organism Homo sapiens(human)
Definition Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017004485.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT 
GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC 
AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT 
GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC 
ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT 
CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT 
CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA 
ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG 
CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA 
CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC 
CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG 
ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT 
CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA 
CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC 
CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT 
ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT 
CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT 
CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT 
TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT 
GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG 
AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA 
TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA 
CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG 
GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA 
AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT 
ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT 
TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG 
CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC 
TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC 
AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA 
AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT 
CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA 
CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT 
CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA 
ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC 
CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA 
GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC 
TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT 
GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC 
ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC 
ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC 
GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG 
ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG 
AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT 
CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT 
TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT 
ATCCTCCAGC CTTCAGCAGG AGAAGGTGAT ATGTCATCAA TAAACAATAC TTTGAGTAAC 
CATCAACTGA CTCATCTACA GTCGCTGTTA AACAACAATC AGATGTTTCC TCCAAATCAG 
CAACAGCAGC AACTTCTCCA GGGGTACCAG AATCTCCAGG CGTTCCAAGG ACAGTCCACA 
ATTCCTTGCC CAGCTAACAA TAACCCCATG GCTTGTCTGT TTCAGAACTT TCAGGTGAGA 
ATGCAGGAAG ATGCAGCTCT CCTAAACAAA AGAATAAGCA CTCAGCCTGG GCTCACAGCA 
CTTCCTGAGA ATCCAAACAC TACACTTCCA CCTTTTCAAG ATACACCTTG TGAGTTGCAA 
CCGAGGATTG ACCCATCTCT TGGTCAACAG GTGAAGGATG GCCTCGTTGT GGGTGGCCCA 
GGTGATGCTT CCGTAGATGC CATTTACAAA GCAGTTGTCG ATGCAGCCAG CAAAGGAATG 
CAGGTTGTCA TCACCACTGC AGTCAACAGT ACAACTCAGA TCAGCCCCAT TCCAGCTCTG 
AGTGCCATGA GTGCCTTCAC TGCCTCAATT GGTGACCCAT TAAATCTCTC CAGTGCTGTC 
AGTGCGGTCA TTCATGGACG GAACATGGGA GGTGTTGATC ATGATGGTAG GCTGAGGAAT 
TCAAGAGGGG CTCGGCTGCC CAAGAATCTA GACCATGGGA AAAATGTGAA CGAAGGAGAT 
GGGTTTGAAT ATTTCAAGTC AGCAAGTTGC CACACATCCA AAAAACAGTG GGACGGGGAG 
CAAAGCCCCA GAGGGGAGCG AAACAGGTGG AAGTACGAGG AATTTTTAGA TCATCCAGGC 
CATATCCACA GTAGTCCTTG TCATGAAAGG CCCAACAATG TCTCTACACT GCCATTTCTG 
CCTGGGGAAC AGCACCCAAT ACTGTTACCA CCAAGAAACT GTCCAGGGGA TAAAATTCTA 
GAGGAAAATT TCAGGTATAA TAACTACAAA AGAACTATGA TGAGTTTTAA GGAGAGACTA 
GAGAACACTG TGGAAAGATG TGCACACATA AATGGGAATA GACCTCGACA GAGTCGGGGA 
TTTGGAGAGC TGCTAAGCAC TGCAAAGCAA GACCTGGTCC TAGAGGAGCA GTCTCCAAGT 
TCCTCAAATA GTTTGGAAAA TTCTCTGGTC AAAGACTACA TCCATTACAA TGGAGACTTT 
AATGCCAAAA GCGTTAATGG GTGTGTGCCT AGCCCTTCAG ATGCTAAAAG CATTAGTAGT 
GAAGATGACC TAAGGAACCC AGACTCCCCC TCTTCAAATG AATTGATACA TTATAGACCA 
AGGACGTTCA ATGTTGGCGA CTTGGTCTGG GGCCAAATCA AAGGACTGAC TTCCTGGCCT 
GGAAAATTAG TAAGAGAAGA CGACGTTCAC AATTCATGTC AACAAAGCCC CGAGGAAGGG 
AAGGTCTGGG TAATGTGGTT TGGTCTTCAT ACCTTCACTC AGGTGGAGCC CGAGAAGTTG 
AAGACACTAA CAGAAGGTTT GGAAGCCTAC AGCCGTGTCC GGAAAAGGAA CAGAAAAAGT 
GGAAAGCTAA ATAACCATTT AGAAGCTGCT ATTCATGAGG CCATGAGTGA ACTGGACAAA 
ATGTCTGGGA CTGTACACCA AATCCCACAG GGTGACAGAC AAATGAGACC CCCCAAACCC 
AAGAGGAGGA AGATCTCCAG ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Diagnostic exome sequencing identifies a heterozygous MBD5 frameshift mutation in a family with intellectual disability and epilepsy.
European journal of medical genetics60(10)559-564(2017 Oct)
Han JY,Lee IG,Jang W,Kim M,Kim Y,Jang JH,Park J


book

MBD5 haploinsufficiency is associated with sleep disturbance and disrupts circadian pathways common to Smith-Magenis and fragile X syndromes.
European journal of human genetics : EJHG23(6)781-9(2015 Jun)
Mullegama SV,Pugliesi L,Burns B,Shah Z,Tahir R,Gu Y,Nelson DL,Elsea SH


book

A molecular model for neurodevelopmental disorders.
Translational psychiatry5e565(2015 May)
Gigek CO,Chen ES,Ota VK,Maussion G,Peng H,Vaillancourt K,Diallo AB,Lopez JP,Crapper L,Vasuta C,Chen GG,Ernst C


book

Phenotypic and molecular convergence of 2q23.1 deletion syndrome with other neurodevelopmental syndromes associated with autism spectrum disorder.
International journal of molecular sciences16(4)7627-43(2015 Apr)
Mullegama SV,Alaimo JT,Chen L,Elsea SH