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PRKCA cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol PRKCA
Entrez Gene ID 5578
Full Name protein kinase C alpha
Synonyms AAG6,PKC-alpha,PKCA,PRKACA
General protein information
Preferred Names

protein kinase C alpha

Names

protein kinase C alpha type
PKC-A
aging-associated gene 6
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

17

17q24.2
Summary Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2008].
Disorder MIM:

176960

Disorder Html: Pituitary tumor, invasive (3)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_017024836.1 XP_016880325.1 protein kinase C alpha type isoform X1
XM_024450830.1 XP_024306598.1 protein kinase C alpha type isoform X3
XM_017024841.1 XP_016880330.1 protein kinase C alpha type isoform X4
NM_002737.3 NP_002728.2 protein kinase C alpha type
NM_002737.2 NP_002728.1 protein kinase C alpha type
XM_017024839.1 XP_016880328.1 protein kinase C alpha type isoform X3
XM_017024840.1 XP_016880329.1 protein kinase C alpha type isoform X3
XM_017024837.1 XP_016880326.1 protein kinase C alpha type isoform X2
XM_024450829.1 XP_024306597.1 protein kinase C alpha type isoform X3
XM_017024838.1 XP_016880327.1 protein kinase C alpha type isoform X3
XM_017024836.2 XP_016880325.1 protein kinase C alpha type isoform X1

Pathways from BioSystems


Homologies


Mus musculus (house mouse) Prkca NP_035231.2
Rattus norvegicus (Norway rat) Prkca NP_001099183.1
Drosophila melanogaster (fruit fly) Pkc53E NP_725626.1
Homo sapiens (human) PRKCA NP_002728.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP012252 XP_320290.4
Canis lupus familiaris (dog) PRKCA XP_005624328.1
Bos taurus (cattle) PRKCA NP_776860.1
Gallus gallus (chicken) PRKCA NP_001012822.1
Danio rerio (zebrafish) prkca NP_001243170.1
PRKCA XP_002800615.1
Gallus gallus (chicken) LOC101749801 XP_004946286.1
Caenorhabditis elegans (roundworm) pkc-2 NP_001024518.1
Pan troglodytes (chimpanzee) PRKCA XP_003315734.1
Xenopus tropicalis (tropical clawed frog) prkca NP_001072752.1

Gene Ontology

The following PRKCA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRKCA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu19098 NM_002737.2
Latest version!		 Homo sapiens protein kinase C alpha (PRKCA), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $189.50-$265.30
$379.00
OHu86982 XM_017024841.1
Latest version!		 Homo sapiens protein kinase C alpha (PRKCA), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $139.30
$199.00
OHu107780 NM_002737.3
Latest version!		 Homo sapiens protein kinase C alpha (PRKCA), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00
OHu86979 XM_017024836.2
Latest version!		 Homo sapiens protein kinase C alpha (PRKCA), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
OHu86980 XM_017024837.1
Latest version!		 Homo sapiens protein kinase C alpha (PRKCA), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
OHu86981 XM_024450829.1
Latest version!		 Homo sapiens protein kinase C alpha (PRKCA), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
OHu86981 XM_017024839.1
Latest version!		 Homo sapiens protein kinase C alpha (PRKCA), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
OHu86981 XM_017024838.1
Latest version!		 Homo sapiens protein kinase C alpha (PRKCA), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
OHu86981 XM_024450830.1
Latest version!		 Homo sapiens protein kinase C alpha (PRKCA), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
OHu86981 XM_017024840.1
Latest version!		 Homo sapiens protein kinase C alpha (PRKCA), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OHu86979 XM_017024836.1 Homo sapiens protein kinase C alpha (PRKCA), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu19098
Clone ID Related Accession (Same CDS sequence) NM_002737.2
Accession Version NM_002737.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2019bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-12-17
Organism Homo sapiens(human)
Product protein kinase C alpha type
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC053321.1, X52479.1, AL596674.1, BQ018414.1, AA017296.1, BM015483.1, AF035594.1, BX648954.1, AF086287.1, BU150006.1, AK055431.1, AK125425.1 and CA417000.1. This sequence is a reference standard in the RefSeqGene project. On May 13, 2004 this sequence version replaced NM_002737.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.10531.1, SRR1660807.61550.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

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1981
ATGGCTGACG TTTTCCCGGG CAACGACTCC ACGGCGTCTC AGGACGTGGC CAACCGCTTC 
GCCCGCAAAG GGGCGCTGAG GCAGAAGAAC GTGCACGAGG TGAAGGACCA CAAATTCATC 
GCGCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGGTTT 
GGGAAACAAG GCTTCCAGTG CCAAGTTTGC TGTTTTGTGG TCCACAAGAG GTGCCATGAA 
TTTGTTACTT TTTCTTGTCC GGGTGCGGAT AAGGGACCCG ACACTGATGA CCCCAGGAGC 
AAGCACAAGT TCAAAATCCA CACTTACGGA AGCCCCACCT TCTGCGATCA CTGTGGGTCA 
CTGCTCTATG GACTTATCCA TCAAGGGATG AAATGTGACA CCTGCGATAT GAACGTTCAC 
AAGCAATGCG TCATCAATGT CCCCAGCCTC TGCGGAATGG ATCACACTGA GAAGAGGGGG 
CGGATTTACC TAAAGGCTGA GGTTGCTGAT GAAAAGCTCC ATGTCACAGT ACGAGATGCA 
AAAAATCTAA TCCCTATGGA TCCAAACGGG CTTTCAGATC CTTATGTGAA GCTGAAACTT 
ATTCCTGATC CCAAGAATGA AAGCAAGCAA AAAACCAAAA CCATCCGCTC CACACTAAAT 
CCGCAGTGGA ATGAGTCCTT TACATTCAAA TTGAAACCTT CAGACAAAGA CCGACGACTG 
TCTGTAGAAA TCTGGGACTG GGATCGAACA ACAAGGAATG ACTTCATGGG ATCCCTTTCC 
TTTGGAGTTT CGGAGCTGAT GAAGATGCCG GCCAGTGGAT GGTACAAGTT GCTTAACCAA 
GAAGAAGGTG AGTACTACAA CGTACCCATT CCGGAAGGGG ACGAGGAAGG AAACATGGAA 
CTCAGGCAGA AATTCGAGAA AGCCAAACTT GGCCCTGCTG GCAACAAAGT CATCAGTCCC 
TCTGAAGACA GGAAACAACC TTCCAACAAC CTTGACCGAG TGAAACTCAC GGACTTCAAT 
TTCCTCATGG TGTTGGGAAA GGGGAGTTTT GGAAAGGTGA TGCTTGCCGA CAGGAAGGGC 
ACAGAAGAAC TGTATGCAAT CAAAATCCTG AAGAAGGATG TGGTGATTCA GGATGATGAC 
GTGGAGTGCA CCATGGTAGA AAAGCGAGTC TTGGCCCTGC TTGACAAACC CCCGTTCTTG 
ACGCAGCTGC ACTCCTGCTT CCAGACAGTG GATCGGCTGT ACTTCGTCAT GGAATATGTC 
AACGGTGGGG ACCTCATGTA CCACATTCAG CAAGTAGGAA AATTTAAGGA ACCACAAGCA 
GTATTCTATG CGGCAGAGAT TTCCATCGGA TTGTTCTTTC TTCATAAAAG AGGAATCATT 
TATAGGGATC TGAAGTTAGA TAACGTCATG TTGGATTCAG AAGGACATAT CAAAATTGCT 
GACTTTGGGA TGTGCAAGGA ACACATGATG GATGGAGTCA CGACCAGGAC CTTCTGTGGG 
ACTCCAGATT ATATCGCCCC AGAGATAATC GCTTATCAGC CGTATGGAAA ATCTGTGGAC 
TGGTGGGCCT ATGGCGTCCT GTTGTATGAA ATGCTTGCCG GGCAGCCTCC ATTTGATGGT 
GAAGATGAAG ACGAGCTATT TCAGTCTATC ATGGAGCACA ACGTTTCCTA TCCAAAATCC 
TTGTCCAAGG AGGCTGTTTC TATCTGCAAA GGACTGATGA CCAAACACCC AGCCAAGCGG 
CTGGGCTGTG GGCCTGAGGG GGAGAGGGAC GTGAGAGAGC ATGCCTTCTT CCGGAGGATC 
GACTGGGAAA AACTGGAGAA CAGGGAGATC CAGCCACCAT TCAAGCCCAA AGTGTGTGGC 
AAAGGAGCAG AGAACTTTGA CAAGTTCTTC ACACGAGGAC AGCCCGTCTT AACACCACCT 
GATCAGCTGG TTATTGCTAA CATAGACCAG TCTGATTTTG AAGGGTTCTC GTATGTCAAC 
CCCCAGTTTG TGCACCCCAT CTTACAGAGT GCAGTATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002728.1
CDS45..2063
Translation

Target ORF information:

RefSeq Version NM_002737.2
Organism Homo sapiens(human)
Definition Homo sapiens protein kinase C alpha (PRKCA), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_002737.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCTGACG TTTTCCCGGG CAACGACTCC ACGGCGTCTC AGGACGTGGC CAACCGCTTC 
GCCCGCAAAG GGGCGCTGAG GCAGAAGAAC GTGCACGAGG TGAAGGACCA CAAATTCATC 
GCGCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGGTTT 
GGGAAACAAG GCTTCCAGTG CCAAGTTTGC TGTTTTGTGG TCCACAAGAG GTGCCATGAA 
TTTGTTACTT TTTCTTGTCC GGGTGCGGAT AAGGGACCCG ACACTGATGA CCCCAGGAGC 
AAGCACAAGT TCAAAATCCA CACTTACGGA AGCCCCACCT TCTGCGATCA CTGTGGGTCA 
CTGCTCTATG GACTTATCCA TCAAGGGATG AAATGTGACA CCTGCGATAT GAACGTTCAC 
AAGCAATGCG TCATCAATGT CCCCAGCCTC TGCGGAATGG ATCACACTGA GAAGAGGGGG 
CGGATTTACC TAAAGGCTGA GGTTGCTGAT GAAAAGCTCC ATGTCACAGT ACGAGATGCA 
AAAAATCTAA TCCCTATGGA TCCAAACGGG CTTTCAGATC CTTATGTGAA GCTGAAACTT 
ATTCCTGATC CCAAGAATGA AAGCAAGCAA AAAACCAAAA CCATCCGCTC CACACTAAAT 
CCGCAGTGGA ATGAGTCCTT TACATTCAAA TTGAAACCTT CAGACAAAGA CCGACGACTG 
TCTGTAGAAA TCTGGGACTG GGATCGAACA ACAAGGAATG ACTTCATGGG ATCCCTTTCC 
TTTGGAGTTT CGGAGCTGAT GAAGATGCCG GCCAGTGGAT GGTACAAGTT GCTTAACCAA 
GAAGAAGGTG AGTACTACAA CGTACCCATT CCGGAAGGGG ACGAGGAAGG AAACATGGAA 
CTCAGGCAGA AATTCGAGAA AGCCAAACTT GGCCCTGCTG GCAACAAAGT CATCAGTCCC 
TCTGAAGACA GGAAACAACC TTCCAACAAC CTTGACCGAG TGAAACTCAC GGACTTCAAT 
TTCCTCATGG TGTTGGGAAA GGGGAGTTTT GGAAAGGTGA TGCTTGCCGA CAGGAAGGGC 
ACAGAAGAAC TGTATGCAAT CAAAATCCTG AAGAAGGATG TGGTGATTCA GGATGATGAC 
GTGGAGTGCA CCATGGTAGA AAAGCGAGTC TTGGCCCTGC TTGACAAACC CCCGTTCTTG 
ACGCAGCTGC ACTCCTGCTT CCAGACAGTG GATCGGCTGT ACTTCGTCAT GGAATATGTC 
AACGGTGGGG ACCTCATGTA CCACATTCAG CAAGTAGGAA AATTTAAGGA ACCACAAGCA 
GTATTCTATG CGGCAGAGAT TTCCATCGGA TTGTTCTTTC TTCATAAAAG AGGAATCATT 
TATAGGGATC TGAAGTTAGA TAACGTCATG TTGGATTCAG AAGGACATAT CAAAATTGCT 
GACTTTGGGA TGTGCAAGGA ACACATGATG GATGGAGTCA CGACCAGGAC CTTCTGTGGG 
ACTCCAGATT ATATCGCCCC AGAGATAATC GCTTATCAGC CGTATGGAAA ATCTGTGGAC 
TGGTGGGCCT ATGGCGTCCT GTTGTATGAA ATGCTTGCCG GGCAGCCTCC ATTTGATGGT 
GAAGATGAAG ACGAGCTATT TCAGTCTATC ATGGAGCACA ACGTTTCCTA TCCAAAATCC 
TTGTCCAAGG AGGCTGTTTC TATCTGCAAA GGACTGATGA CCAAACACCC AGCCAAGCGG 
CTGGGCTGTG GGCCTGAGGG GGAGAGGGAC GTGAGAGAGC ATGCCTTCTT CCGGAGGATC 
GACTGGGAAA AACTGGAGAA CAGGGAGATC CAGCCACCAT TCAAGCCCAA AGTGTGTGGC 
AAAGGAGCAG AGAACTTTGA CAAGTTCTTC ACACGAGGAC AGCCCGTCTT AACACCACCT 
GATCAGCTGG TTATTGCTAA CATAGACCAG TCTGATTTTG AAGGGTTCTC GTATGTCAAC 
CCCCAGTTTG TGCACCCCAT CTTACAGAGT GCAGTATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu86982
Clone ID Related Accession (Same CDS sequence) XM_017024841.1
Accession Version XM_017024841.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 975bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product protein kinase C alpha type isoform X4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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961
ATGGCTGACG TTTTCCCGGG CAACGACTCC ACGGCGTCTC AGGACGTGGC CAACCGCTTC 
GCCCGCAAAG GGGCGCTGAG GCAGAAGAAC GTGCACGAGG TGAAGGACCA CAAATTCATC 
GCGCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGGTTT 
GGGAAACAAG GCTTCCAGTG CCAAGTTTGC TGTTTTGTGG TCCACAAGAG GTGCCATGAA 
TTTGTTACTT TTTCTTGTCC GGGTGCGGAT AAGGGACCCG ACACTGATGA CCCCAGGAGC 
AAGCACAAGT TCAAAATCCA CACTTACGGA AGCCCCACCT TCTGCGATCA CTGTGGGTCA 
CTGCTCTATG GACTTATCCA TCAAGGGATG AAATGTGACA CCTGCGATAT GAACGTTCAC 
AAGCAATGCG TCATCAATGT CCCCAGCCTC TGCGGAATGG ATCACACTGA GAAGAGGGGG 
CGGATTTACC TAAAGGCTGA GGTTGCTGAT GAAAAGCTCC ATGTCACAGT ACGAGATGCA 
AAAAATCTAA TCCCTATGGA TCCAAACGGG CTTTCAGATC CTTATGTGAA GCTGAAACTT 
ATTCCTGATC CCAAGAATGA AAGCAAGCAA AAAACCAAAA CCATCCGCTC CACACTAAAT 
CCGCAGTGGA ATGAGTCCTT TACATTCAAA TTGAAACCTT CAGACAAAGA CCGACGACTG 
TCTGTAGAAA TCTGGGACTG GGATCGAACA ACAAGGAATG ACTTCATGGG ATCCCTTTCC 
TTTGGAGTTT CGGAGCTGAT GAAGATGCCG GCCAGTGGAT GGTACAAGTT GCTTAACCAA 
GAAGAAGGTG AGTACTACAA CGTACCCATT CCGGAAGGGG ACGAGGAAGG AAACATGGAA 
CTCAGGCAGA AATTCGAGCT GAGGATGCAG ACCTGGGGTA CTGGACAGGA CCACCTTCTT 
GAGCCCCATC CCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016880330.1
CDS213..1187
Translation

Target ORF information:

RefSeq Version XM_017024841.1
Organism Homo sapiens(human)
Definition Homo sapiens protein kinase C alpha (PRKCA), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017024841.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTGACG TTTTCCCGGG CAACGACTCC ACGGCGTCTC AGGACGTGGC CAACCGCTTC 
GCCCGCAAAG GGGCGCTGAG GCAGAAGAAC GTGCACGAGG TGAAGGACCA CAAATTCATC 
GCGCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGGTTT 
GGGAAACAAG GCTTCCAGTG CCAAGTTTGC TGTTTTGTGG TCCACAAGAG GTGCCATGAA 
TTTGTTACTT TTTCTTGTCC GGGTGCGGAT AAGGGACCCG ACACTGATGA CCCCAGGAGC 
AAGCACAAGT TCAAAATCCA CACTTACGGA AGCCCCACCT TCTGCGATCA CTGTGGGTCA 
CTGCTCTATG GACTTATCCA TCAAGGGATG AAATGTGACA CCTGCGATAT GAACGTTCAC 
AAGCAATGCG TCATCAATGT CCCCAGCCTC TGCGGAATGG ATCACACTGA GAAGAGGGGG 
CGGATTTACC TAAAGGCTGA GGTTGCTGAT GAAAAGCTCC ATGTCACAGT ACGAGATGCA 
AAAAATCTAA TCCCTATGGA TCCAAACGGG CTTTCAGATC CTTATGTGAA GCTGAAACTT 
ATTCCTGATC CCAAGAATGA AAGCAAGCAA AAAACCAAAA CCATCCGCTC CACACTAAAT 
CCGCAGTGGA ATGAGTCCTT TACATTCAAA TTGAAACCTT CAGACAAAGA CCGACGACTG 
TCTGTAGAAA TCTGGGACTG GGATCGAACA ACAAGGAATG ACTTCATGGG ATCCCTTTCC 
TTTGGAGTTT CGGAGCTGAT GAAGATGCCG GCCAGTGGAT GGTACAAGTT GCTTAACCAA 
GAAGAAGGTG AGTACTACAA CGTACCCATT CCGGAAGGGG ACGAGGAAGG AAACATGGAA 
CTCAGGCAGA AATTCGAGCT GAGGATGCAG ACCTGGGGTA CTGGACAGGA CCACCTTCTT 
GAGCCCCATC CCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu107780
Clone ID Related Accession (Same CDS sequence) NM_002737.3
Accession Version NM_002737.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2019bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-30
Organism Homo sapiens(human)
Product protein kinase C alpha type
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009452.21, AC006263.1, AC006947.2, AC005918.13 and AC005988.1. On Nov 23, 2018 this sequence version replaced NM_002737.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.10531.1, SRR1660807.61550.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000413366.8/ ENSP00000408695.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCTGACG TTTTCCCGGG CAACGACTCC ACGGCGTCTC AGGACGTGGC CAACCGCTTC 
GCCCGCAAAG GGGCGCTGAG GCAGAAGAAC GTGCACGAGG TGAAGGACCA CAAATTCATC 
GCGCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGGTTT 
GGGAAACAAG GCTTCCAGTG CCAAGTTTGC TGTTTTGTGG TCCACAAGAG GTGCCATGAA 
TTTGTTACTT TTTCTTGTCC GGGTGCGGAT AAGGGACCCG ACACTGATGA CCCCAGGAGC 
AAGCACAAGT TCAAAATCCA CACTTACGGA AGCCCCACCT TCTGCGATCA CTGTGGGTCA 
CTGCTCTATG GACTTATCCA TCAAGGGATG AAATGTGACA CCTGCGATAT GAACGTTCAC 
AAGCAATGCG TCATCAATGT CCCCAGCCTC TGCGGAATGG ATCACACTGA GAAGAGGGGG 
CGGATTTACC TAAAGGCTGA GGTTGCTGAT GAAAAGCTCC ATGTCACAGT ACGAGATGCA 
AAAAATCTAA TCCCTATGGA TCCAAACGGG CTTTCAGATC CTTATGTGAA GCTGAAACTT 
ATTCCTGATC CCAAGAATGA AAGCAAGCAA AAAACCAAAA CCATCCGCTC CACACTAAAT 
CCGCAGTGGA ATGAGTCCTT TACATTCAAA TTGAAACCTT CAGACAAAGA CCGACGACTG 
TCTGTAGAAA TCTGGGACTG GGATCGAACA ACAAGGAATG ACTTCATGGG ATCCCTTTCC 
TTTGGAGTTT CGGAGCTGAT GAAGATGCCG GCCAGTGGAT GGTACAAGTT GCTTAACCAA 
GAAGAAGGTG AGTACTACAA CGTACCCATT CCGGAAGGGG ACGAGGAAGG AAACATGGAA 
CTCAGGCAGA AATTCGAGAA AGCCAAACTT GGCCCTGCTG GCAACAAAGT CATCAGTCCC 
TCTGAAGACA GGAAACAACC TTCCAACAAC CTTGACCGAG TGAAACTCAC GGACTTCAAT 
TTCCTCATGG TGTTGGGAAA GGGGAGTTTT GGAAAGGTGA TGCTTGCCGA CAGGAAGGGC 
ACAGAAGAAC TGTATGCAAT CAAAATCCTG AAGAAGGATG TGGTGATTCA GGATGATGAC 
GTGGAGTGCA CCATGGTAGA AAAGCGAGTC TTGGCCCTGC TTGACAAACC CCCGTTCTTG 
ACGCAGCTGC ACTCCTGCTT CCAGACAGTG GATCGGCTGT ACTTCGTCAT GGAATATGTC 
AACGGTGGGG ACCTCATGTA CCACATTCAG CAAGTAGGAA AATTTAAGGA ACCACAAGCA 
GTATTCTATG CGGCAGAGAT TTCCATCGGA TTGTTCTTTC TTCATAAAAG AGGAATCATT 
TATAGGGATC TGAAGTTAGA TAACGTCATG TTGGATTCAG AAGGACATAT CAAAATTGCT 
GACTTTGGGA TGTGCAAGGA ACACATGATG GATGGAGTCA CGACCAGGAC CTTCTGTGGG 
ACTCCAGATT ATATCGCCCC AGAGATAATC GCTTATCAGC CGTATGGAAA ATCTGTGGAC 
TGGTGGGCCT ATGGCGTCCT GTTGTATGAA ATGCTTGCCG GGCAGCCTCC ATTTGATGGT 
GAAGATGAAG ACGAGCTATT TCAGTCTATC ATGGAGCACA ACGTTTCCTA TCCAAAATCC 
TTGTCCAAGG AGGCTGTTTC TGTCTGCAAA GGACTGATGA CCAAACACCC AGCCAAGCGG 
CTGGGCTGTG GGCCTGAGGG GGAGAGGGAC GTGAGAGAGC ATGCCTTCTT CCGGAGGATC 
GACTGGGAAA AACTGGAGAA CAGGGAGATC CAGCCACCAT TCAAGCCCAA AGTGTGTGGC 
AAAGGAGCAG AGAACTTTGA CAAGTTCTTC ACACGAGGAC AGCCCGTCTT AACACCACCT 
GATCAGCTGG TTATTGCTAA CATAGACCAG TCTGATTTTG AAGGGTTCTC GTATGTCAAC 
CCCCAGTTTG TGCACCCCAT CTTACAGAGT GCAGTATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002728.2
CDS240..2258
Translation

Target ORF information:

RefSeq Version NM_002737.3
Organism Homo sapiens(human)
Definition Homo sapiens protein kinase C alpha (PRKCA), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_002737.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCTGACG TTTTCCCGGG CAACGACTCC ACGGCGTCTC AGGACGTGGC CAACCGCTTC 
GCCCGCAAAG GGGCGCTGAG GCAGAAGAAC GTGCACGAGG TGAAGGACCA CAAATTCATC 
GCGCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGGTTT 
GGGAAACAAG GCTTCCAGTG CCAAGTTTGC TGTTTTGTGG TCCACAAGAG GTGCCATGAA 
TTTGTTACTT TTTCTTGTCC GGGTGCGGAT AAGGGACCCG ACACTGATGA CCCCAGGAGC 
AAGCACAAGT TCAAAATCCA CACTTACGGA AGCCCCACCT TCTGCGATCA CTGTGGGTCA 
CTGCTCTATG GACTTATCCA TCAAGGGATG AAATGTGACA CCTGCGATAT GAACGTTCAC 
AAGCAATGCG TCATCAATGT CCCCAGCCTC TGCGGAATGG ATCACACTGA GAAGAGGGGG 
CGGATTTACC TAAAGGCTGA GGTTGCTGAT GAAAAGCTCC ATGTCACAGT ACGAGATGCA 
AAAAATCTAA TCCCTATGGA TCCAAACGGG CTTTCAGATC CTTATGTGAA GCTGAAACTT 
ATTCCTGATC CCAAGAATGA AAGCAAGCAA AAAACCAAAA CCATCCGCTC CACACTAAAT 
CCGCAGTGGA ATGAGTCCTT TACATTCAAA TTGAAACCTT CAGACAAAGA CCGACGACTG 
TCTGTAGAAA TCTGGGACTG GGATCGAACA ACAAGGAATG ACTTCATGGG ATCCCTTTCC 
TTTGGAGTTT CGGAGCTGAT GAAGATGCCG GCCAGTGGAT GGTACAAGTT GCTTAACCAA 
GAAGAAGGTG AGTACTACAA CGTACCCATT CCGGAAGGGG ACGAGGAAGG AAACATGGAA 
CTCAGGCAGA AATTCGAGAA AGCCAAACTT GGCCCTGCTG GCAACAAAGT CATCAGTCCC 
TCTGAAGACA GGAAACAACC TTCCAACAAC CTTGACCGAG TGAAACTCAC GGACTTCAAT 
TTCCTCATGG TGTTGGGAAA GGGGAGTTTT GGAAAGGTGA TGCTTGCCGA CAGGAAGGGC 
ACAGAAGAAC TGTATGCAAT CAAAATCCTG AAGAAGGATG TGGTGATTCA GGATGATGAC 
GTGGAGTGCA CCATGGTAGA AAAGCGAGTC TTGGCCCTGC TTGACAAACC CCCGTTCTTG 
ACGCAGCTGC ACTCCTGCTT CCAGACAGTG GATCGGCTGT ACTTCGTCAT GGAATATGTC 
AACGGTGGGG ACCTCATGTA CCACATTCAG CAAGTAGGAA AATTTAAGGA ACCACAAGCA 
GTATTCTATG CGGCAGAGAT TTCCATCGGA TTGTTCTTTC TTCATAAAAG AGGAATCATT 
TATAGGGATC TGAAGTTAGA TAACGTCATG TTGGATTCAG AAGGACATAT CAAAATTGCT 
GACTTTGGGA TGTGCAAGGA ACACATGATG GATGGAGTCA CGACCAGGAC CTTCTGTGGG 
ACTCCAGATT ATATCGCCCC AGAGATAATC GCTTATCAGC CGTATGGAAA ATCTGTGGAC 
TGGTGGGCCT ATGGCGTCCT GTTGTATGAA ATGCTTGCCG GGCAGCCTCC ATTTGATGGT 
GAAGATGAAG ACGAGCTATT TCAGTCTATC ATGGAGCACA ACGTTTCCTA TCCAAAATCC 
TTGTCCAAGG AGGCTGTTTC TGTCTGCAAA GGACTGATGA CCAAACACCC AGCCAAGCGG 
CTGGGCTGTG GGCCTGAGGG GGAGAGGGAC GTGAGAGAGC ATGCCTTCTT CCGGAGGATC 
GACTGGGAAA AACTGGAGAA CAGGGAGATC CAGCCACCAT TCAAGCCCAA AGTGTGTGGC 
AAAGGAGCAG AGAACTTTGA CAAGTTCTTC ACACGAGGAC AGCCCGTCTT AACACCACCT 
GATCAGCTGG TTATTGCTAA CATAGACCAG TCTGATTTTG AAGGGTTCTC GTATGTCAAC 
CCCCAGTTTG TGCACCCCAT CTTACAGAGT GCAGTATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu86979
Clone ID Related Accession (Same CDS sequence) XM_017024836.1 , XM_017024836.2
Accession Version XM_017024836.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1938bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-05
Organism Homo sapiens(human)
Product protein kinase C alpha type isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCTGACG TTTTCCCGGG CAACGACTCC ACGGCGTCTC AGGACGTGGC CAACCGCTTC 
GCCCGCAAAG GGGCGCTGAG GCAGAAGAAC GTGCACGAGG TGAAGGACCA CAAATTCATC 
GCGCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGGTTT 
GGGAAACAAG GCTTCCAGTG CCAAGTTTGC TGTTTTGTGG TCCACAAGAG GTGCCATGAA 
TTTGTTACTT TTTCTTGTCC GGGTGCGGAT AAGGGACCCG ACACTGATGA CCCCAGGAGC 
AAGCACAAGT TCAAAATCCA CACTTACGGA AGCCCCACCT TCTGCGATCA CTGTGGGTCA 
CTGCTCTATG GACTTATCCA TCAAGGGATG AAATGTGACA CCTGCGATAT GAACGTTCAC 
AAGCAATGCG TCATCAATGT CCCCAGCCTC TGCGGAATGG ATCACACTGA GAAGAGGGGG 
CGGATTTACC TAAAGGCTGA GGTTGCTGAT GAAAAGCTCC ATGTCACAGT ACGAGATGCA 
AAAAATCTAA TCCCTATGGA TCCAAACGGG CTTTCAGATC CTTATGTGAA GCTGAAACTT 
ATTCCTGATC CCAAGAATGA AAGCAAGCAA AAAACCAAAA CCATCCGCTC CACACTAAAT 
CCGCAGTGGA ATGAGTCCTT TACATTCAAA TTGAAACCTT CAGACAAAGA CCGACGACTG 
TCTGTAGAAA TCTGGGACTG GGATCGAACA ACAAGGAATG ACTTCATGGG ATCCCTTTCC 
TTTGGAGTTT CGGAGCTGAT GAAGATGCCG GCCAGTGGAT GGTACAAGTT GCTTAACCAA 
GAAGAAGGTG AGTACTACAA CGTACCCATT CCGGAAGGGG ACGAGGAAGG AAACATGGAA 
CTCAGGCAGA AATTCGAGAA AGCCAAACTT GGCCCTGCTG GCAACAAAGT CATCAGTCCC 
TCTGAAGACA GGAAACAACC TTCCAACAAC CTTGACCGAG TGAAACTCAC GGACTTCAAT 
TTCCTCATGG TGTTGGGAAA GGGGAGTTTT GGAAAGGTGA TGCTTGCCGA CAGGAAGGGC 
ACAGAAGAAC TGTATGCAAT CAAAATCCTG AAGAAGGATG TGGTGATTCA GGATGATGAC 
GTGGAGTGCA CCATGGTAGA AAAGCGAGTC TTGGCCCTGC TTGACAAACC CCCGTTCTTG 
ACGCAGCTGC ACTCCTGCTT CCAGACAGTG GATCGGCTGT ACTTCGTCAT GGAATATGTC 
AACGGTGGGG ACCTCATGTA CCACATTCAG CAAGTAGGAA AATTTAAGGA ACCACAAGCA 
GTATTCTATG CGGCAGAGAT TTCCATCGGA TTGTTCTTTC TTCATAAAAG AGGAATCATT 
TATAGGGATC TGAAGTTAGA TAACGTCATG TTGGATTCAG AAGGACATAT CAAAATTGCT 
GACTTTGGGA TGTGCAAGGA ACACATGATG GATGGAGTCA CGACCAGGAC CTTCTGTGGG 
ACTCCAGATT ATATCGCCCC AGAGATAATC GCTTATCAGC CGTATGGAAA ATCTGTGGAC 
TGGTGGGCCT ATGGCGTCCT GTTGTATGAA ATGCTTGCCG GGCAGCCTCC ATTTGATGGT 
GAAGATGAAG ACGAGCTATT TCAGTCTATC ATGGAGCACA ACGTTTCCTA TCCAAAATCC 
TTGTCCAAGG AGGCTGTTTC TGTCTGCAAA GGACTGATGA CCAAACACCC AGCCAAGCGG 
CTGGGCTGTG GGCCTGAGGG GGAGAGGGAC GTGAGAGAGC ATGCCTTCTT CCGGAGGATC 
GACTGGGAAA AACTGGAGAA CAGGGAGATC CAGCCACCAT TCAAGCCCAA AGTGACTTTG 
TGTACCAAAA TGCACTGGCT TCAGTGGGCC TCCAGGTCTT CGGTGAGCAC GTTTCCAGAA 
CCTTGGGTGA CTAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016880325.1
CDS213..2150
Translation

Target ORF information:

RefSeq Version XM_017024836.1
Organism Homo sapiens(human)
Definition Homo sapiens protein kinase C alpha (PRKCA), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017024836.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCTGACG TTTTCCCGGG CAACGACTCC ACGGCGTCTC AGGACGTGGC CAACCGCTTC 
GCCCGCAAAG GGGCGCTGAG GCAGAAGAAC GTGCACGAGG TGAAGGACCA CAAATTCATC 
GCGCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGGTTT 
GGGAAACAAG GCTTCCAGTG CCAAGTTTGC TGTTTTGTGG TCCACAAGAG GTGCCATGAA 
TTTGTTACTT TTTCTTGTCC GGGTGCGGAT AAGGGACCCG ACACTGATGA CCCCAGGAGC 
AAGCACAAGT TCAAAATCCA CACTTACGGA AGCCCCACCT TCTGCGATCA CTGTGGGTCA 
CTGCTCTATG GACTTATCCA TCAAGGGATG AAATGTGACA CCTGCGATAT GAACGTTCAC 
AAGCAATGCG TCATCAATGT CCCCAGCCTC TGCGGAATGG ATCACACTGA GAAGAGGGGG 
CGGATTTACC TAAAGGCTGA GGTTGCTGAT GAAAAGCTCC ATGTCACAGT ACGAGATGCA 
AAAAATCTAA TCCCTATGGA TCCAAACGGG CTTTCAGATC CTTATGTGAA GCTGAAACTT 
ATTCCTGATC CCAAGAATGA AAGCAAGCAA AAAACCAAAA CCATCCGCTC CACACTAAAT 
CCGCAGTGGA ATGAGTCCTT TACATTCAAA TTGAAACCTT CAGACAAAGA CCGACGACTG 
TCTGTAGAAA TCTGGGACTG GGATCGAACA ACAAGGAATG ACTTCATGGG ATCCCTTTCC 
TTTGGAGTTT CGGAGCTGAT GAAGATGCCG GCCAGTGGAT GGTACAAGTT GCTTAACCAA 
GAAGAAGGTG AGTACTACAA CGTACCCATT CCGGAAGGGG ACGAGGAAGG AAACATGGAA 
CTCAGGCAGA AATTCGAGAA AGCCAAACTT GGCCCTGCTG GCAACAAAGT CATCAGTCCC 
TCTGAAGACA GGAAACAACC TTCCAACAAC CTTGACCGAG TGAAACTCAC GGACTTCAAT 
TTCCTCATGG TGTTGGGAAA GGGGAGTTTT GGAAAGGTGA TGCTTGCCGA CAGGAAGGGC 
ACAGAAGAAC TGTATGCAAT CAAAATCCTG AAGAAGGATG TGGTGATTCA GGATGATGAC 
GTGGAGTGCA CCATGGTAGA AAAGCGAGTC TTGGCCCTGC TTGACAAACC CCCGTTCTTG 
ACGCAGCTGC ACTCCTGCTT CCAGACAGTG GATCGGCTGT ACTTCGTCAT GGAATATGTC 
AACGGTGGGG ACCTCATGTA CCACATTCAG CAAGTAGGAA AATTTAAGGA ACCACAAGCA 
GTATTCTATG CGGCAGAGAT TTCCATCGGA TTGTTCTTTC TTCATAAAAG AGGAATCATT 
TATAGGGATC TGAAGTTAGA TAACGTCATG TTGGATTCAG AAGGACATAT CAAAATTGCT 
GACTTTGGGA TGTGCAAGGA ACACATGATG GATGGAGTCA CGACCAGGAC CTTCTGTGGG 
ACTCCAGATT ATATCGCCCC AGAGATAATC GCTTATCAGC CGTATGGAAA ATCTGTGGAC 
TGGTGGGCCT ATGGCGTCCT GTTGTATGAA ATGCTTGCCG GGCAGCCTCC ATTTGATGGT 
GAAGATGAAG ACGAGCTATT TCAGTCTATC ATGGAGCACA ACGTTTCCTA TCCAAAATCC 
TTGTCCAAGG AGGCTGTTTC TGTCTGCAAA GGACTGATGA CCAAACACCC AGCCAAGCGG 
CTGGGCTGTG GGCCTGAGGG GGAGAGGGAC GTGAGAGAGC ATGCCTTCTT CCGGAGGATC 
GACTGGGAAA AACTGGAGAA CAGGGAGATC CAGCCACCAT TCAAGCCCAA AGTGACTTTG 
TGTACCAAAA TGCACTGGCT TCAGTGGGCC TCCAGGTCTT CGGTGAGCAC GTTTCCAGAA 
CCTTGGGTGA CTAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu86979
Clone ID Related Accession (Same CDS sequence) XM_017024836.1 , XM_017024836.2
Accession Version XM_017024836.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1938bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product protein kinase C alpha type isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017024836.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCTGACG TTTTCCCGGG CAACGACTCC ACGGCGTCTC AGGACGTGGC CAACCGCTTC 
GCCCGCAAAG GGGCGCTGAG GCAGAAGAAC GTGCACGAGG TGAAGGACCA CAAATTCATC 
GCGCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGGTTT 
GGGAAACAAG GCTTCCAGTG CCAAGTTTGC TGTTTTGTGG TCCACAAGAG GTGCCATGAA 
TTTGTTACTT TTTCTTGTCC GGGTGCGGAT AAGGGACCCG ACACTGATGA CCCCAGGAGC 
AAGCACAAGT TCAAAATCCA CACTTACGGA AGCCCCACCT TCTGCGATCA CTGTGGGTCA 
CTGCTCTATG GACTTATCCA TCAAGGGATG AAATGTGACA CCTGCGATAT GAACGTTCAC 
AAGCAATGCG TCATCAATGT CCCCAGCCTC TGCGGAATGG ATCACACTGA GAAGAGGGGG 
CGGATTTACC TAAAGGCTGA GGTTGCTGAT GAAAAGCTCC ATGTCACAGT ACGAGATGCA 
AAAAATCTAA TCCCTATGGA TCCAAACGGG CTTTCAGATC CTTATGTGAA GCTGAAACTT 
ATTCCTGATC CCAAGAATGA AAGCAAGCAA AAAACCAAAA CCATCCGCTC CACACTAAAT 
CCGCAGTGGA ATGAGTCCTT TACATTCAAA TTGAAACCTT CAGACAAAGA CCGACGACTG 
TCTGTAGAAA TCTGGGACTG GGATCGAACA ACAAGGAATG ACTTCATGGG ATCCCTTTCC 
TTTGGAGTTT CGGAGCTGAT GAAGATGCCG GCCAGTGGAT GGTACAAGTT GCTTAACCAA 
GAAGAAGGTG AGTACTACAA CGTACCCATT CCGGAAGGGG ACGAGGAAGG AAACATGGAA 
CTCAGGCAGA AATTCGAGAA AGCCAAACTT GGCCCTGCTG GCAACAAAGT CATCAGTCCC 
TCTGAAGACA GGAAACAACC TTCCAACAAC CTTGACCGAG TGAAACTCAC GGACTTCAAT 
TTCCTCATGG TGTTGGGAAA GGGGAGTTTT GGAAAGGTGA TGCTTGCCGA CAGGAAGGGC 
ACAGAAGAAC TGTATGCAAT CAAAATCCTG AAGAAGGATG TGGTGATTCA GGATGATGAC 
GTGGAGTGCA CCATGGTAGA AAAGCGAGTC TTGGCCCTGC TTGACAAACC CCCGTTCTTG 
ACGCAGCTGC ACTCCTGCTT CCAGACAGTG GATCGGCTGT ACTTCGTCAT GGAATATGTC 
AACGGTGGGG ACCTCATGTA CCACATTCAG CAAGTAGGAA AATTTAAGGA ACCACAAGCA 
GTATTCTATG CGGCAGAGAT TTCCATCGGA TTGTTCTTTC TTCATAAAAG AGGAATCATT 
TATAGGGATC TGAAGTTAGA TAACGTCATG TTGGATTCAG AAGGACATAT CAAAATTGCT 
GACTTTGGGA TGTGCAAGGA ACACATGATG GATGGAGTCA CGACCAGGAC CTTCTGTGGG 
ACTCCAGATT ATATCGCCCC AGAGATAATC GCTTATCAGC CGTATGGAAA ATCTGTGGAC 
TGGTGGGCCT ATGGCGTCCT GTTGTATGAA ATGCTTGCCG GGCAGCCTCC ATTTGATGGT 
GAAGATGAAG ACGAGCTATT TCAGTCTATC ATGGAGCACA ACGTTTCCTA TCCAAAATCC 
TTGTCCAAGG AGGCTGTTTC TGTCTGCAAA GGACTGATGA CCAAACACCC AGCCAAGCGG 
CTGGGCTGTG GGCCTGAGGG GGAGAGGGAC GTGAGAGAGC ATGCCTTCTT CCGGAGGATC 
GACTGGGAAA AACTGGAGAA CAGGGAGATC CAGCCACCAT TCAAGCCCAA AGTGACTTTG 
TGTACCAAAA TGCACTGGCT TCAGTGGGCC TCCAGGTCTT CGGTGAGCAC GTTTCCAGAA 
CCTTGGGTGA CTAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016880325.1
CDS213..2150
Translation

Target ORF information:

RefSeq Version XM_017024836.2
Organism Homo sapiens(human)
Definition Homo sapiens protein kinase C alpha (PRKCA), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017024836.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCTGACG TTTTCCCGGG CAACGACTCC ACGGCGTCTC AGGACGTGGC CAACCGCTTC 
GCCCGCAAAG GGGCGCTGAG GCAGAAGAAC GTGCACGAGG TGAAGGACCA CAAATTCATC 
GCGCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGGTTT 
GGGAAACAAG GCTTCCAGTG CCAAGTTTGC TGTTTTGTGG TCCACAAGAG GTGCCATGAA 
TTTGTTACTT TTTCTTGTCC GGGTGCGGAT AAGGGACCCG ACACTGATGA CCCCAGGAGC 
AAGCACAAGT TCAAAATCCA CACTTACGGA AGCCCCACCT TCTGCGATCA CTGTGGGTCA 
CTGCTCTATG GACTTATCCA TCAAGGGATG AAATGTGACA CCTGCGATAT GAACGTTCAC 
AAGCAATGCG TCATCAATGT CCCCAGCCTC TGCGGAATGG ATCACACTGA GAAGAGGGGG 
CGGATTTACC TAAAGGCTGA GGTTGCTGAT GAAAAGCTCC ATGTCACAGT ACGAGATGCA 
AAAAATCTAA TCCCTATGGA TCCAAACGGG CTTTCAGATC CTTATGTGAA GCTGAAACTT 
ATTCCTGATC CCAAGAATGA AAGCAAGCAA AAAACCAAAA CCATCCGCTC CACACTAAAT 
CCGCAGTGGA ATGAGTCCTT TACATTCAAA TTGAAACCTT CAGACAAAGA CCGACGACTG 
TCTGTAGAAA TCTGGGACTG GGATCGAACA ACAAGGAATG ACTTCATGGG ATCCCTTTCC 
TTTGGAGTTT CGGAGCTGAT GAAGATGCCG GCCAGTGGAT GGTACAAGTT GCTTAACCAA 
GAAGAAGGTG AGTACTACAA CGTACCCATT CCGGAAGGGG ACGAGGAAGG AAACATGGAA 
CTCAGGCAGA AATTCGAGAA AGCCAAACTT GGCCCTGCTG GCAACAAAGT CATCAGTCCC 
TCTGAAGACA GGAAACAACC TTCCAACAAC CTTGACCGAG TGAAACTCAC GGACTTCAAT 
TTCCTCATGG TGTTGGGAAA GGGGAGTTTT GGAAAGGTGA TGCTTGCCGA CAGGAAGGGC 
ACAGAAGAAC TGTATGCAAT CAAAATCCTG AAGAAGGATG TGGTGATTCA GGATGATGAC 
GTGGAGTGCA CCATGGTAGA AAAGCGAGTC TTGGCCCTGC TTGACAAACC CCCGTTCTTG 
ACGCAGCTGC ACTCCTGCTT CCAGACAGTG GATCGGCTGT ACTTCGTCAT GGAATATGTC 
AACGGTGGGG ACCTCATGTA CCACATTCAG CAAGTAGGAA AATTTAAGGA ACCACAAGCA 
GTATTCTATG CGGCAGAGAT TTCCATCGGA TTGTTCTTTC TTCATAAAAG AGGAATCATT 
TATAGGGATC TGAAGTTAGA TAACGTCATG TTGGATTCAG AAGGACATAT CAAAATTGCT 
GACTTTGGGA TGTGCAAGGA ACACATGATG GATGGAGTCA CGACCAGGAC CTTCTGTGGG 
ACTCCAGATT ATATCGCCCC AGAGATAATC GCTTATCAGC CGTATGGAAA ATCTGTGGAC 
TGGTGGGCCT ATGGCGTCCT GTTGTATGAA ATGCTTGCCG GGCAGCCTCC ATTTGATGGT 
GAAGATGAAG ACGAGCTATT TCAGTCTATC ATGGAGCACA ACGTTTCCTA TCCAAAATCC 
TTGTCCAAGG AGGCTGTTTC TGTCTGCAAA GGACTGATGA CCAAACACCC AGCCAAGCGG 
CTGGGCTGTG GGCCTGAGGG GGAGAGGGAC GTGAGAGAGC ATGCCTTCTT CCGGAGGATC 
GACTGGGAAA AACTGGAGAA CAGGGAGATC CAGCCACCAT TCAAGCCCAA AGTGACTTTG 
TGTACCAAAA TGCACTGGCT TCAGTGGGCC TCCAGGTCTT CGGTGAGCAC GTTTCCAGAA 
CCTTGGGTGA CTAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu86980
Clone ID Related Accession (Same CDS sequence) XM_017024837.1
Accession Version XM_017024837.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1866bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product protein kinase C alpha type isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGCAGCCAG CGTCGGCCAA CCTCCAAGCT CCTCTTTCCT GTTCCCCTCA CGTTTGCTGT 
TTTGTGGTCC ACAAGAGGTG CCATGAATTT GTTACTTTTT CTTGTCCGGG TGCGGATAAG 
GGACCCGACA CTGATGACCC CAGGAGCAAG CACAAGTTCA AAATCCACAC TTACGGAAGC 
CCCACCTTCT GCGATCACTG TGGGTCACTG CTCTATGGAC TTATCCATCA AGGGATGAAA 
TGTGACACCT GCGATATGAA CGTTCACAAG CAATGCGTCA TCAATGTCCC CAGCCTCTGC 
GGAATGGATC ACACTGAGAA GAGGGGGCGG ATTTACCTAA AGGCTGAGGT TGCTGATGAA 
AAGCTCCATG TCACAGTACG AGATGCAAAA AATCTAATCC CTATGGATCC AAACGGGCTT 
TCAGATCCTT ATGTGAAGCT GAAACTTATT CCTGATCCCA AGAATGAAAG CAAGCAAAAA 
ACCAAAACCA TCCGCTCCAC ACTAAATCCG CAGTGGAATG AGTCCTTTAC ATTCAAATTG 
AAACCTTCAG ACAAAGACCG ACGACTGTCT GTAGAAATCT GGGACTGGGA TCGAACAACA 
AGGAATGACT TCATGGGATC CCTTTCCTTT GGAGTTTCGG AGCTGATGAA GATGCCGGCC 
AGTGGATGGT ACAAGTTGCT TAACCAAGAA GAAGGTGAGT ACTACAACGT ACCCATTCCG 
GAAGGGGACG AGGAAGGAAA CATGGAACTC AGGCAGAAAT TCGAGAAAGC CAAACTTGGC 
CCTGCTGGCA ACAAAGTCAT CAGTCCCTCT GAAGACAGGA AACAACCTTC CAACAACCTT 
GACCGAGTGA AACTCACGGA CTTCAATTTC CTCATGGTGT TGGGAAAGGG GAGTTTTGGA 
AAGGTGATGC TTGCCGACAG GAAGGGCACA GAAGAACTGT ATGCAATCAA AATCCTGAAG 
AAGGATGTGG TGATTCAGGA TGATGACGTG GAGTGCACCA TGGTAGAAAA GCGAGTCTTG 
GCCCTGCTTG ACAAACCCCC GTTCTTGACG CAGCTGCACT CCTGCTTCCA GACAGTGGAT 
CGGCTGTACT TCGTCATGGA ATATGTCAAC GGTGGGGACC TCATGTACCA CATTCAGCAA 
GTAGGAAAAT TTAAGGAACC ACAAGCAGTA TTCTATGCGG CAGAGATTTC CATCGGATTG 
TTCTTTCTTC ATAAAAGAGG AATCATTTAT AGGGATCTGA AGTTAGATAA CGTCATGTTG 
GATTCAGAAG GACATATCAA AATTGCTGAC TTTGGGATGT GCAAGGAACA CATGATGGAT 
GGAGTCACGA CCAGGACCTT CTGTGGGACT CCAGATTATA TCGCCCCAGA GATAATCGCT 
TATCAGCCGT ATGGAAAATC TGTGGACTGG TGGGCCTATG GCGTCCTGTT GTATGAAATG 
CTTGCCGGGC AGCCTCCATT TGATGGTGAA GATGAAGACG AGCTATTTCA GTCTATCATG 
GAGCACAACG TTTCCTATCC AAAATCCTTG TCCAAGGAGG CTGTTTCTGT CTGCAAAGGA 
CTGATGACCA AACACCCAGC CAAGCGGCTG GGCTGTGGGC CTGAGGGGGA GAGGGACGTG 
AGAGAGCATG CCTTCTTCCG GAGGATCGAC TGGGAAAAAC TGGAGAACAG GGAGATCCAG 
CCACCATTCA AGCCCAAAGT GTGTGGCAAA GGAGCAGAGA ACTTTGACAA GTTCTTCACA 
CGAGGACAGC CCGTCTTAAC ACCACCTGAT CAGCTGGTTA TTGCTAACAT AGACCAGTCT 
GATTTTGAAG GGTTCTCGTA TGTCAACCCC CAGTTTGTGC ACCCCATCTT ACAGAGTGCA 
GTATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016880326.1
CDS29..1894
Translation

Target ORF information:

RefSeq Version XM_017024837.1
Organism Homo sapiens(human)
Definition Homo sapiens protein kinase C alpha (PRKCA), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017024837.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGCAGCCAG CGTCGGCCAA CCTCCAAGCT CCTCTTTCCT GTTCCCCTCA CGTTTGCTGT 
TTTGTGGTCC ACAAGAGGTG CCATGAATTT GTTACTTTTT CTTGTCCGGG TGCGGATAAG 
GGACCCGACA CTGATGACCC CAGGAGCAAG CACAAGTTCA AAATCCACAC TTACGGAAGC 
CCCACCTTCT GCGATCACTG TGGGTCACTG CTCTATGGAC TTATCCATCA AGGGATGAAA 
TGTGACACCT GCGATATGAA CGTTCACAAG CAATGCGTCA TCAATGTCCC CAGCCTCTGC 
GGAATGGATC ACACTGAGAA GAGGGGGCGG ATTTACCTAA AGGCTGAGGT TGCTGATGAA 
AAGCTCCATG TCACAGTACG AGATGCAAAA AATCTAATCC CTATGGATCC AAACGGGCTT 
TCAGATCCTT ATGTGAAGCT GAAACTTATT CCTGATCCCA AGAATGAAAG CAAGCAAAAA 
ACCAAAACCA TCCGCTCCAC ACTAAATCCG CAGTGGAATG AGTCCTTTAC ATTCAAATTG 
AAACCTTCAG ACAAAGACCG ACGACTGTCT GTAGAAATCT GGGACTGGGA TCGAACAACA 
AGGAATGACT TCATGGGATC CCTTTCCTTT GGAGTTTCGG AGCTGATGAA GATGCCGGCC 
AGTGGATGGT ACAAGTTGCT TAACCAAGAA GAAGGTGAGT ACTACAACGT ACCCATTCCG 
GAAGGGGACG AGGAAGGAAA CATGGAACTC AGGCAGAAAT TCGAGAAAGC CAAACTTGGC 
CCTGCTGGCA ACAAAGTCAT CAGTCCCTCT GAAGACAGGA AACAACCTTC CAACAACCTT 
GACCGAGTGA AACTCACGGA CTTCAATTTC CTCATGGTGT TGGGAAAGGG GAGTTTTGGA 
AAGGTGATGC TTGCCGACAG GAAGGGCACA GAAGAACTGT ATGCAATCAA AATCCTGAAG 
AAGGATGTGG TGATTCAGGA TGATGACGTG GAGTGCACCA TGGTAGAAAA GCGAGTCTTG 
GCCCTGCTTG ACAAACCCCC GTTCTTGACG CAGCTGCACT CCTGCTTCCA GACAGTGGAT 
CGGCTGTACT TCGTCATGGA ATATGTCAAC GGTGGGGACC TCATGTACCA CATTCAGCAA 
GTAGGAAAAT TTAAGGAACC ACAAGCAGTA TTCTATGCGG CAGAGATTTC CATCGGATTG 
TTCTTTCTTC ATAAAAGAGG AATCATTTAT AGGGATCTGA AGTTAGATAA CGTCATGTTG 
GATTCAGAAG GACATATCAA AATTGCTGAC TTTGGGATGT GCAAGGAACA CATGATGGAT 
GGAGTCACGA CCAGGACCTT CTGTGGGACT CCAGATTATA TCGCCCCAGA GATAATCGCT 
TATCAGCCGT ATGGAAAATC TGTGGACTGG TGGGCCTATG GCGTCCTGTT GTATGAAATG 
CTTGCCGGGC AGCCTCCATT TGATGGTGAA GATGAAGACG AGCTATTTCA GTCTATCATG 
GAGCACAACG TTTCCTATCC AAAATCCTTG TCCAAGGAGG CTGTTTCTGT CTGCAAAGGA 
CTGATGACCA AACACCCAGC CAAGCGGCTG GGCTGTGGGC CTGAGGGGGA GAGGGACGTG 
AGAGAGCATG CCTTCTTCCG GAGGATCGAC TGGGAAAAAC TGGAGAACAG GGAGATCCAG 
CCACCATTCA AGCCCAAAGT GTGTGGCAAA GGAGCAGAGA ACTTTGACAA GTTCTTCACA 
CGAGGACAGC CCGTCTTAAC ACCACCTGAT CAGCTGGTTA TTGCTAACAT AGACCAGTCT 
GATTTTGAAG GGTTCTCGTA TGTCAACCCC CAGTTTGTGC ACCCCATCTT ACAGAGTGCA 
GTATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu86981
Clone ID Related Accession (Same CDS sequence) XM_024450830.1 , XM_017024839.1 , XM_017024840.1 , XM_024450829.1 , XM_017024838.1
Accession Version XM_024450830.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1761bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product protein kinase C alpha type isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGCTAAGTG TGTCATCCTG GGATTCAAAC GACCCCAGGA GCAAGCACAA GTTCAAAATC 
CACACTTACG GAAGCCCCAC CTTCTGCGAT CACTGTGGGT CACTGCTCTA TGGACTTATC 
CATCAAGGGA TGAAATGTGA CACCTGCGAT ATGAACGTTC ACAAGCAATG CGTCATCAAT 
GTCCCCAGCC TCTGCGGAAT GGATCACACT GAGAAGAGGG GGCGGATTTA CCTAAAGGCT 
GAGGTTGCTG ATGAAAAGCT CCATGTCACA GTACGAGATG CAAAAAATCT AATCCCTATG 
GATCCAAACG GGCTTTCAGA TCCTTATGTG AAGCTGAAAC TTATTCCTGA TCCCAAGAAT 
GAAAGCAAGC AAAAAACCAA AACCATCCGC TCCACACTAA ATCCGCAGTG GAATGAGTCC 
TTTACATTCA AATTGAAACC TTCAGACAAA GACCGACGAC TGTCTGTAGA AATCTGGGAC 
TGGGATCGAA CAACAAGGAA TGACTTCATG GGATCCCTTT CCTTTGGAGT TTCGGAGCTG 
ATGAAGATGC CGGCCAGTGG ATGGTACAAG TTGCTTAACC AAGAAGAAGG TGAGTACTAC 
AACGTACCCA TTCCGGAAGG GGACGAGGAA GGAAACATGG AACTCAGGCA GAAATTCGAG 
AAAGCCAAAC TTGGCCCTGC TGGCAACAAA GTCATCAGTC CCTCTGAAGA CAGGAAACAA 
CCTTCCAACA ACCTTGACCG AGTGAAACTC ACGGACTTCA ATTTCCTCAT GGTGTTGGGA 
AAGGGGAGTT TTGGAAAGGT GATGCTTGCC GACAGGAAGG GCACAGAAGA ACTGTATGCA 
ATCAAAATCC TGAAGAAGGA TGTGGTGATT CAGGATGATG ACGTGGAGTG CACCATGGTA 
GAAAAGCGAG TCTTGGCCCT GCTTGACAAA CCCCCGTTCT TGACGCAGCT GCACTCCTGC 
TTCCAGACAG TGGATCGGCT GTACTTCGTC ATGGAATATG TCAACGGTGG GGACCTCATG 
TACCACATTC AGCAAGTAGG AAAATTTAAG GAACCACAAG CAGTATTCTA TGCGGCAGAG 
ATTTCCATCG GATTGTTCTT TCTTCATAAA AGAGGAATCA TTTATAGGGA TCTGAAGTTA 
GATAACGTCA TGTTGGATTC AGAAGGACAT ATCAAAATTG CTGACTTTGG GATGTGCAAG 
GAACACATGA TGGATGGAGT CACGACCAGG ACCTTCTGTG GGACTCCAGA TTATATCGCC 
CCAGAGATAA TCGCTTATCA GCCGTATGGA AAATCTGTGG ACTGGTGGGC CTATGGCGTC 
CTGTTGTATG AAATGCTTGC CGGGCAGCCT CCATTTGATG GTGAAGATGA AGACGAGCTA 
TTTCAGTCTA TCATGGAGCA CAACGTTTCC TATCCAAAAT CCTTGTCCAA GGAGGCTGTT 
TCTGTCTGCA AAGGACTGAT GACCAAACAC CCAGCCAAGC GGCTGGGCTG TGGGCCTGAG 
GGGGAGAGGG ACGTGAGAGA GCATGCCTTC TTCCGGAGGA TCGACTGGGA AAAACTGGAG 
AACAGGGAGA TCCAGCCACC ATTCAAGCCC AAAGTGTGTG GCAAAGGAGC AGAGAACTTT 
GACAAGTTCT TCACACGAGG ACAGCCCGTC TTAACACCAC CTGATCAGCT GGTTATTGCT 
AACATAGACC AGTCTGATTT TGAAGGGTTC TCGTATGTCA ACCCCCAGTT TGTGCACCCC 
ATCTTACAGA GTGCAGTATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_024306598.1
CDS151..1911
Translation

Target ORF information:

RefSeq Version XM_024450830.1
Organism Homo sapiens(human)
Definition Homo sapiens protein kinase C alpha (PRKCA), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_024450830.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGCTAAGTG TGTCATCCTG GGATTCAAAC GACCCCAGGA GCAAGCACAA GTTCAAAATC 
CACACTTACG GAAGCCCCAC CTTCTGCGAT CACTGTGGGT CACTGCTCTA TGGACTTATC 
CATCAAGGGA TGAAATGTGA CACCTGCGAT ATGAACGTTC ACAAGCAATG CGTCATCAAT 
GTCCCCAGCC TCTGCGGAAT GGATCACACT GAGAAGAGGG GGCGGATTTA CCTAAAGGCT 
GAGGTTGCTG ATGAAAAGCT CCATGTCACA GTACGAGATG CAAAAAATCT AATCCCTATG 
GATCCAAACG GGCTTTCAGA TCCTTATGTG AAGCTGAAAC TTATTCCTGA TCCCAAGAAT 
GAAAGCAAGC AAAAAACCAA AACCATCCGC TCCACACTAA ATCCGCAGTG GAATGAGTCC 
TTTACATTCA AATTGAAACC TTCAGACAAA GACCGACGAC TGTCTGTAGA AATCTGGGAC 
TGGGATCGAA CAACAAGGAA TGACTTCATG GGATCCCTTT CCTTTGGAGT TTCGGAGCTG 
ATGAAGATGC CGGCCAGTGG ATGGTACAAG TTGCTTAACC AAGAAGAAGG TGAGTACTAC 
AACGTACCCA TTCCGGAAGG GGACGAGGAA GGAAACATGG AACTCAGGCA GAAATTCGAG 
AAAGCCAAAC TTGGCCCTGC TGGCAACAAA GTCATCAGTC CCTCTGAAGA CAGGAAACAA 
CCTTCCAACA ACCTTGACCG AGTGAAACTC ACGGACTTCA ATTTCCTCAT GGTGTTGGGA 
AAGGGGAGTT TTGGAAAGGT GATGCTTGCC GACAGGAAGG GCACAGAAGA ACTGTATGCA 
ATCAAAATCC TGAAGAAGGA TGTGGTGATT CAGGATGATG ACGTGGAGTG CACCATGGTA 
GAAAAGCGAG TCTTGGCCCT GCTTGACAAA CCCCCGTTCT TGACGCAGCT GCACTCCTGC 
TTCCAGACAG TGGATCGGCT GTACTTCGTC ATGGAATATG TCAACGGTGG GGACCTCATG 
TACCACATTC AGCAAGTAGG AAAATTTAAG GAACCACAAG CAGTATTCTA TGCGGCAGAG 
ATTTCCATCG GATTGTTCTT TCTTCATAAA AGAGGAATCA TTTATAGGGA TCTGAAGTTA 
GATAACGTCA TGTTGGATTC AGAAGGACAT ATCAAAATTG CTGACTTTGG GATGTGCAAG 
GAACACATGA TGGATGGAGT CACGACCAGG ACCTTCTGTG GGACTCCAGA TTATATCGCC 
CCAGAGATAA TCGCTTATCA GCCGTATGGA AAATCTGTGG ACTGGTGGGC CTATGGCGTC 
CTGTTGTATG AAATGCTTGC CGGGCAGCCT CCATTTGATG GTGAAGATGA AGACGAGCTA 
TTTCAGTCTA TCATGGAGCA CAACGTTTCC TATCCAAAAT CCTTGTCCAA GGAGGCTGTT 
TCTGTCTGCA AAGGACTGAT GACCAAACAC CCAGCCAAGC GGCTGGGCTG TGGGCCTGAG 
GGGGAGAGGG ACGTGAGAGA GCATGCCTTC TTCCGGAGGA TCGACTGGGA AAAACTGGAG 
AACAGGGAGA TCCAGCCACC ATTCAAGCCC AAAGTGTGTG GCAAAGGAGC AGAGAACTTT 
GACAAGTTCT TCACACGAGG ACAGCCCGTC TTAACACCAC CTGATCAGCT GGTTATTGCT 
AACATAGACC AGTCTGATTT TGAAGGGTTC TCGTATGTCA ACCCCCAGTT TGTGCACCCC 
ATCTTACAGA GTGCAGTATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu86981
Clone ID Related Accession (Same CDS sequence) XM_024450830.1 , XM_017024839.1 , XM_017024840.1 , XM_024450829.1 , XM_017024838.1
Accession Version XM_017024839.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1761bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-05
Organism Homo sapiens(human)
Product protein kinase C alpha type isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGCTAAGTG TGTCATCCTG GGATTCAAAC GACCCCAGGA GCAAGCACAA GTTCAAAATC 
CACACTTACG GAAGCCCCAC CTTCTGCGAT CACTGTGGGT CACTGCTCTA TGGACTTATC 
CATCAAGGGA TGAAATGTGA CACCTGCGAT ATGAACGTTC ACAAGCAATG CGTCATCAAT 
GTCCCCAGCC TCTGCGGAAT GGATCACACT GAGAAGAGGG GGCGGATTTA CCTAAAGGCT 
GAGGTTGCTG ATGAAAAGCT CCATGTCACA GTACGAGATG CAAAAAATCT AATCCCTATG 
GATCCAAACG GGCTTTCAGA TCCTTATGTG AAGCTGAAAC TTATTCCTGA TCCCAAGAAT 
GAAAGCAAGC AAAAAACCAA AACCATCCGC TCCACACTAA ATCCGCAGTG GAATGAGTCC 
TTTACATTCA AATTGAAACC TTCAGACAAA GACCGACGAC TGTCTGTAGA AATCTGGGAC 
TGGGATCGAA CAACAAGGAA TGACTTCATG GGATCCCTTT CCTTTGGAGT TTCGGAGCTG 
ATGAAGATGC CGGCCAGTGG ATGGTACAAG TTGCTTAACC AAGAAGAAGG TGAGTACTAC 
AACGTACCCA TTCCGGAAGG GGACGAGGAA GGAAACATGG AACTCAGGCA GAAATTCGAG 
AAAGCCAAAC TTGGCCCTGC TGGCAACAAA GTCATCAGTC CCTCTGAAGA CAGGAAACAA 
CCTTCCAACA ACCTTGACCG AGTGAAACTC ACGGACTTCA ATTTCCTCAT GGTGTTGGGA 
AAGGGGAGTT TTGGAAAGGT GATGCTTGCC GACAGGAAGG GCACAGAAGA ACTGTATGCA 
ATCAAAATCC TGAAGAAGGA TGTGGTGATT CAGGATGATG ACGTGGAGTG CACCATGGTA 
GAAAAGCGAG TCTTGGCCCT GCTTGACAAA CCCCCGTTCT TGACGCAGCT GCACTCCTGC 
TTCCAGACAG TGGATCGGCT GTACTTCGTC ATGGAATATG TCAACGGTGG GGACCTCATG 
TACCACATTC AGCAAGTAGG AAAATTTAAG GAACCACAAG CAGTATTCTA TGCGGCAGAG 
ATTTCCATCG GATTGTTCTT TCTTCATAAA AGAGGAATCA TTTATAGGGA TCTGAAGTTA 
GATAACGTCA TGTTGGATTC AGAAGGACAT ATCAAAATTG CTGACTTTGG GATGTGCAAG 
GAACACATGA TGGATGGAGT CACGACCAGG ACCTTCTGTG GGACTCCAGA TTATATCGCC 
CCAGAGATAA TCGCTTATCA GCCGTATGGA AAATCTGTGG ACTGGTGGGC CTATGGCGTC 
CTGTTGTATG AAATGCTTGC CGGGCAGCCT CCATTTGATG GTGAAGATGA AGACGAGCTA 
TTTCAGTCTA TCATGGAGCA CAACGTTTCC TATCCAAAAT CCTTGTCCAA GGAGGCTGTT 
TCTGTCTGCA AAGGACTGAT GACCAAACAC CCAGCCAAGC GGCTGGGCTG TGGGCCTGAG 
GGGGAGAGGG ACGTGAGAGA GCATGCCTTC TTCCGGAGGA TCGACTGGGA AAAACTGGAG 
AACAGGGAGA TCCAGCCACC ATTCAAGCCC AAAGTGTGTG GCAAAGGAGC AGAGAACTTT 
GACAAGTTCT TCACACGAGG ACAGCCCGTC TTAACACCAC CTGATCAGCT GGTTATTGCT 
AACATAGACC AGTCTGATTT TGAAGGGTTC TCGTATGTCA ACCCCCAGTT TGTGCACCCC 
ATCTTACAGA GTGCAGTATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016880328.1
CDS1169..2929
Translation

Target ORF information:

RefSeq Version XM_017024839.1
Organism Homo sapiens(human)
Definition Homo sapiens protein kinase C alpha (PRKCA), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017024839.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGCTAAGTG TGTCATCCTG GGATTCAAAC GACCCCAGGA GCAAGCACAA GTTCAAAATC 
CACACTTACG GAAGCCCCAC CTTCTGCGAT CACTGTGGGT CACTGCTCTA TGGACTTATC 
CATCAAGGGA TGAAATGTGA CACCTGCGAT ATGAACGTTC ACAAGCAATG CGTCATCAAT 
GTCCCCAGCC TCTGCGGAAT GGATCACACT GAGAAGAGGG GGCGGATTTA CCTAAAGGCT 
GAGGTTGCTG ATGAAAAGCT CCATGTCACA GTACGAGATG CAAAAAATCT AATCCCTATG 
GATCCAAACG GGCTTTCAGA TCCTTATGTG AAGCTGAAAC TTATTCCTGA TCCCAAGAAT 
GAAAGCAAGC AAAAAACCAA AACCATCCGC TCCACACTAA ATCCGCAGTG GAATGAGTCC 
TTTACATTCA AATTGAAACC TTCAGACAAA GACCGACGAC TGTCTGTAGA AATCTGGGAC 
TGGGATCGAA CAACAAGGAA TGACTTCATG GGATCCCTTT CCTTTGGAGT TTCGGAGCTG 
ATGAAGATGC CGGCCAGTGG ATGGTACAAG TTGCTTAACC AAGAAGAAGG TGAGTACTAC 
AACGTACCCA TTCCGGAAGG GGACGAGGAA GGAAACATGG AACTCAGGCA GAAATTCGAG 
AAAGCCAAAC TTGGCCCTGC TGGCAACAAA GTCATCAGTC CCTCTGAAGA CAGGAAACAA 
CCTTCCAACA ACCTTGACCG AGTGAAACTC ACGGACTTCA ATTTCCTCAT GGTGTTGGGA 
AAGGGGAGTT TTGGAAAGGT GATGCTTGCC GACAGGAAGG GCACAGAAGA ACTGTATGCA 
ATCAAAATCC TGAAGAAGGA TGTGGTGATT CAGGATGATG ACGTGGAGTG CACCATGGTA 
GAAAAGCGAG TCTTGGCCCT GCTTGACAAA CCCCCGTTCT TGACGCAGCT GCACTCCTGC 
TTCCAGACAG TGGATCGGCT GTACTTCGTC ATGGAATATG TCAACGGTGG GGACCTCATG 
TACCACATTC AGCAAGTAGG AAAATTTAAG GAACCACAAG CAGTATTCTA TGCGGCAGAG 
ATTTCCATCG GATTGTTCTT TCTTCATAAA AGAGGAATCA TTTATAGGGA TCTGAAGTTA 
GATAACGTCA TGTTGGATTC AGAAGGACAT ATCAAAATTG CTGACTTTGG GATGTGCAAG 
GAACACATGA TGGATGGAGT CACGACCAGG ACCTTCTGTG GGACTCCAGA TTATATCGCC 
CCAGAGATAA TCGCTTATCA GCCGTATGGA AAATCTGTGG ACTGGTGGGC CTATGGCGTC 
CTGTTGTATG AAATGCTTGC CGGGCAGCCT CCATTTGATG GTGAAGATGA AGACGAGCTA 
TTTCAGTCTA TCATGGAGCA CAACGTTTCC TATCCAAAAT CCTTGTCCAA GGAGGCTGTT 
TCTGTCTGCA AAGGACTGAT GACCAAACAC CCAGCCAAGC GGCTGGGCTG TGGGCCTGAG 
GGGGAGAGGG ACGTGAGAGA GCATGCCTTC TTCCGGAGGA TCGACTGGGA AAAACTGGAG 
AACAGGGAGA TCCAGCCACC ATTCAAGCCC AAAGTGTGTG GCAAAGGAGC AGAGAACTTT 
GACAAGTTCT TCACACGAGG ACAGCCCGTC TTAACACCAC CTGATCAGCT GGTTATTGCT 
AACATAGACC AGTCTGATTT TGAAGGGTTC TCGTATGTCA ACCCCCAGTT TGTGCACCCC 
ATCTTACAGA GTGCAGTATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu86981
Clone ID Related Accession (Same CDS sequence) XM_024450830.1 , XM_017024839.1 , XM_017024840.1 , XM_024450829.1 , XM_017024838.1
Accession Version XM_017024840.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1761bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-05
Organism Homo sapiens(human)
Product protein kinase C alpha type isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGCTAAGTG TGTCATCCTG GGATTCAAAC GACCCCAGGA GCAAGCACAA GTTCAAAATC 
CACACTTACG GAAGCCCCAC CTTCTGCGAT CACTGTGGGT CACTGCTCTA TGGACTTATC 
CATCAAGGGA TGAAATGTGA CACCTGCGAT ATGAACGTTC ACAAGCAATG CGTCATCAAT 
GTCCCCAGCC TCTGCGGAAT GGATCACACT GAGAAGAGGG GGCGGATTTA CCTAAAGGCT 
GAGGTTGCTG ATGAAAAGCT CCATGTCACA GTACGAGATG CAAAAAATCT AATCCCTATG 
GATCCAAACG GGCTTTCAGA TCCTTATGTG AAGCTGAAAC TTATTCCTGA TCCCAAGAAT 
GAAAGCAAGC AAAAAACCAA AACCATCCGC TCCACACTAA ATCCGCAGTG GAATGAGTCC 
TTTACATTCA AATTGAAACC TTCAGACAAA GACCGACGAC TGTCTGTAGA AATCTGGGAC 
TGGGATCGAA CAACAAGGAA TGACTTCATG GGATCCCTTT CCTTTGGAGT TTCGGAGCTG 
ATGAAGATGC CGGCCAGTGG ATGGTACAAG TTGCTTAACC AAGAAGAAGG TGAGTACTAC 
AACGTACCCA TTCCGGAAGG GGACGAGGAA GGAAACATGG AACTCAGGCA GAAATTCGAG 
AAAGCCAAAC TTGGCCCTGC TGGCAACAAA GTCATCAGTC CCTCTGAAGA CAGGAAACAA 
CCTTCCAACA ACCTTGACCG AGTGAAACTC ACGGACTTCA ATTTCCTCAT GGTGTTGGGA 
AAGGGGAGTT TTGGAAAGGT GATGCTTGCC GACAGGAAGG GCACAGAAGA ACTGTATGCA 
ATCAAAATCC TGAAGAAGGA TGTGGTGATT CAGGATGATG ACGTGGAGTG CACCATGGTA 
GAAAAGCGAG TCTTGGCCCT GCTTGACAAA CCCCCGTTCT TGACGCAGCT GCACTCCTGC 
TTCCAGACAG TGGATCGGCT GTACTTCGTC ATGGAATATG TCAACGGTGG GGACCTCATG 
TACCACATTC AGCAAGTAGG AAAATTTAAG GAACCACAAG CAGTATTCTA TGCGGCAGAG 
ATTTCCATCG GATTGTTCTT TCTTCATAAA AGAGGAATCA TTTATAGGGA TCTGAAGTTA 
GATAACGTCA TGTTGGATTC AGAAGGACAT ATCAAAATTG CTGACTTTGG GATGTGCAAG 
GAACACATGA TGGATGGAGT CACGACCAGG ACCTTCTGTG GGACTCCAGA TTATATCGCC 
CCAGAGATAA TCGCTTATCA GCCGTATGGA AAATCTGTGG ACTGGTGGGC CTATGGCGTC 
CTGTTGTATG AAATGCTTGC CGGGCAGCCT CCATTTGATG GTGAAGATGA AGACGAGCTA 
TTTCAGTCTA TCATGGAGCA CAACGTTTCC TATCCAAAAT CCTTGTCCAA GGAGGCTGTT 
TCTGTCTGCA AAGGACTGAT GACCAAACAC CCAGCCAAGC GGCTGGGCTG TGGGCCTGAG 
GGGGAGAGGG ACGTGAGAGA GCATGCCTTC TTCCGGAGGA TCGACTGGGA AAAACTGGAG 
AACAGGGAGA TCCAGCCACC ATTCAAGCCC AAAGTGTGTG GCAAAGGAGC AGAGAACTTT 
GACAAGTTCT TCACACGAGG ACAGCCCGTC TTAACACCAC CTGATCAGCT GGTTATTGCT 
AACATAGACC AGTCTGATTT TGAAGGGTTC TCGTATGTCA ACCCCCAGTT TGTGCACCCC 
ATCTTACAGA GTGCAGTATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016880329.1
CDS212..1972
Translation

Target ORF information:

RefSeq Version XM_017024840.1
Organism Homo sapiens(human)
Definition Homo sapiens protein kinase C alpha (PRKCA), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017024840.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGCTAAGTG TGTCATCCTG GGATTCAAAC GACCCCAGGA GCAAGCACAA GTTCAAAATC 
CACACTTACG GAAGCCCCAC CTTCTGCGAT CACTGTGGGT CACTGCTCTA TGGACTTATC 
CATCAAGGGA TGAAATGTGA CACCTGCGAT ATGAACGTTC ACAAGCAATG CGTCATCAAT 
GTCCCCAGCC TCTGCGGAAT GGATCACACT GAGAAGAGGG GGCGGATTTA CCTAAAGGCT 
GAGGTTGCTG ATGAAAAGCT CCATGTCACA GTACGAGATG CAAAAAATCT AATCCCTATG 
GATCCAAACG GGCTTTCAGA TCCTTATGTG AAGCTGAAAC TTATTCCTGA TCCCAAGAAT 
GAAAGCAAGC AAAAAACCAA AACCATCCGC TCCACACTAA ATCCGCAGTG GAATGAGTCC 
TTTACATTCA AATTGAAACC TTCAGACAAA GACCGACGAC TGTCTGTAGA AATCTGGGAC 
TGGGATCGAA CAACAAGGAA TGACTTCATG GGATCCCTTT CCTTTGGAGT TTCGGAGCTG 
ATGAAGATGC CGGCCAGTGG ATGGTACAAG TTGCTTAACC AAGAAGAAGG TGAGTACTAC 
AACGTACCCA TTCCGGAAGG GGACGAGGAA GGAAACATGG AACTCAGGCA GAAATTCGAG 
AAAGCCAAAC TTGGCCCTGC TGGCAACAAA GTCATCAGTC CCTCTGAAGA CAGGAAACAA 
CCTTCCAACA ACCTTGACCG AGTGAAACTC ACGGACTTCA ATTTCCTCAT GGTGTTGGGA 
AAGGGGAGTT TTGGAAAGGT GATGCTTGCC GACAGGAAGG GCACAGAAGA ACTGTATGCA 
ATCAAAATCC TGAAGAAGGA TGTGGTGATT CAGGATGATG ACGTGGAGTG CACCATGGTA 
GAAAAGCGAG TCTTGGCCCT GCTTGACAAA CCCCCGTTCT TGACGCAGCT GCACTCCTGC 
TTCCAGACAG TGGATCGGCT GTACTTCGTC ATGGAATATG TCAACGGTGG GGACCTCATG 
TACCACATTC AGCAAGTAGG AAAATTTAAG GAACCACAAG CAGTATTCTA TGCGGCAGAG 
ATTTCCATCG GATTGTTCTT TCTTCATAAA AGAGGAATCA TTTATAGGGA TCTGAAGTTA 
GATAACGTCA TGTTGGATTC AGAAGGACAT ATCAAAATTG CTGACTTTGG GATGTGCAAG 
GAACACATGA TGGATGGAGT CACGACCAGG ACCTTCTGTG GGACTCCAGA TTATATCGCC 
CCAGAGATAA TCGCTTATCA GCCGTATGGA AAATCTGTGG ACTGGTGGGC CTATGGCGTC 
CTGTTGTATG AAATGCTTGC CGGGCAGCCT CCATTTGATG GTGAAGATGA AGACGAGCTA 
TTTCAGTCTA TCATGGAGCA CAACGTTTCC TATCCAAAAT CCTTGTCCAA GGAGGCTGTT 
TCTGTCTGCA AAGGACTGAT GACCAAACAC CCAGCCAAGC GGCTGGGCTG TGGGCCTGAG 
GGGGAGAGGG ACGTGAGAGA GCATGCCTTC TTCCGGAGGA TCGACTGGGA AAAACTGGAG 
AACAGGGAGA TCCAGCCACC ATTCAAGCCC AAAGTGTGTG GCAAAGGAGC AGAGAACTTT 
GACAAGTTCT TCACACGAGG ACAGCCCGTC TTAACACCAC CTGATCAGCT GGTTATTGCT 
AACATAGACC AGTCTGATTT TGAAGGGTTC TCGTATGTCA ACCCCCAGTT TGTGCACCCC 
ATCTTACAGA GTGCAGTATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu86981
Clone ID Related Accession (Same CDS sequence) XM_024450830.1 , XM_017024839.1 , XM_017024840.1 , XM_024450829.1 , XM_017024838.1
Accession Version XM_024450829.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1761bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product protein kinase C alpha type isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGCTAAGTG TGTCATCCTG GGATTCAAAC GACCCCAGGA GCAAGCACAA GTTCAAAATC 
CACACTTACG GAAGCCCCAC CTTCTGCGAT CACTGTGGGT CACTGCTCTA TGGACTTATC 
CATCAAGGGA TGAAATGTGA CACCTGCGAT ATGAACGTTC ACAAGCAATG CGTCATCAAT 
GTCCCCAGCC TCTGCGGAAT GGATCACACT GAGAAGAGGG GGCGGATTTA CCTAAAGGCT 
GAGGTTGCTG ATGAAAAGCT CCATGTCACA GTACGAGATG CAAAAAATCT AATCCCTATG 
GATCCAAACG GGCTTTCAGA TCCTTATGTG AAGCTGAAAC TTATTCCTGA TCCCAAGAAT 
GAAAGCAAGC AAAAAACCAA AACCATCCGC TCCACACTAA ATCCGCAGTG GAATGAGTCC 
TTTACATTCA AATTGAAACC TTCAGACAAA GACCGACGAC TGTCTGTAGA AATCTGGGAC 
TGGGATCGAA CAACAAGGAA TGACTTCATG GGATCCCTTT CCTTTGGAGT TTCGGAGCTG 
ATGAAGATGC CGGCCAGTGG ATGGTACAAG TTGCTTAACC AAGAAGAAGG TGAGTACTAC 
AACGTACCCA TTCCGGAAGG GGACGAGGAA GGAAACATGG AACTCAGGCA GAAATTCGAG 
AAAGCCAAAC TTGGCCCTGC TGGCAACAAA GTCATCAGTC CCTCTGAAGA CAGGAAACAA 
CCTTCCAACA ACCTTGACCG AGTGAAACTC ACGGACTTCA ATTTCCTCAT GGTGTTGGGA 
AAGGGGAGTT TTGGAAAGGT GATGCTTGCC GACAGGAAGG GCACAGAAGA ACTGTATGCA 
ATCAAAATCC TGAAGAAGGA TGTGGTGATT CAGGATGATG ACGTGGAGTG CACCATGGTA 
GAAAAGCGAG TCTTGGCCCT GCTTGACAAA CCCCCGTTCT TGACGCAGCT GCACTCCTGC 
TTCCAGACAG TGGATCGGCT GTACTTCGTC ATGGAATATG TCAACGGTGG GGACCTCATG 
TACCACATTC AGCAAGTAGG AAAATTTAAG GAACCACAAG CAGTATTCTA TGCGGCAGAG 
ATTTCCATCG GATTGTTCTT TCTTCATAAA AGAGGAATCA TTTATAGGGA TCTGAAGTTA 
GATAACGTCA TGTTGGATTC AGAAGGACAT ATCAAAATTG CTGACTTTGG GATGTGCAAG 
GAACACATGA TGGATGGAGT CACGACCAGG ACCTTCTGTG GGACTCCAGA TTATATCGCC 
CCAGAGATAA TCGCTTATCA GCCGTATGGA AAATCTGTGG ACTGGTGGGC CTATGGCGTC 
CTGTTGTATG AAATGCTTGC CGGGCAGCCT CCATTTGATG GTGAAGATGA AGACGAGCTA 
TTTCAGTCTA TCATGGAGCA CAACGTTTCC TATCCAAAAT CCTTGTCCAA GGAGGCTGTT 
TCTGTCTGCA AAGGACTGAT GACCAAACAC CCAGCCAAGC GGCTGGGCTG TGGGCCTGAG 
GGGGAGAGGG ACGTGAGAGA GCATGCCTTC TTCCGGAGGA TCGACTGGGA AAAACTGGAG 
AACAGGGAGA TCCAGCCACC ATTCAAGCCC AAAGTGTGTG GCAAAGGAGC AGAGAACTTT 
GACAAGTTCT TCACACGAGG ACAGCCCGTC TTAACACCAC CTGATCAGCT GGTTATTGCT 
AACATAGACC AGTCTGATTT TGAAGGGTTC TCGTATGTCA ACCCCCAGTT TGTGCACCCC 
ATCTTACAGA GTGCAGTATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_024306597.1
CDS1069..2829
Translation

Target ORF information:

RefSeq Version XM_024450829.1
Organism Homo sapiens(human)
Definition Homo sapiens protein kinase C alpha (PRKCA), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_024450829.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGCTAAGTG TGTCATCCTG GGATTCAAAC GACCCCAGGA GCAAGCACAA GTTCAAAATC 
CACACTTACG GAAGCCCCAC CTTCTGCGAT CACTGTGGGT CACTGCTCTA TGGACTTATC 
CATCAAGGGA TGAAATGTGA CACCTGCGAT ATGAACGTTC ACAAGCAATG CGTCATCAAT 
GTCCCCAGCC TCTGCGGAAT GGATCACACT GAGAAGAGGG GGCGGATTTA CCTAAAGGCT 
GAGGTTGCTG ATGAAAAGCT CCATGTCACA GTACGAGATG CAAAAAATCT AATCCCTATG 
GATCCAAACG GGCTTTCAGA TCCTTATGTG AAGCTGAAAC TTATTCCTGA TCCCAAGAAT 
GAAAGCAAGC AAAAAACCAA AACCATCCGC TCCACACTAA ATCCGCAGTG GAATGAGTCC 
TTTACATTCA AATTGAAACC TTCAGACAAA GACCGACGAC TGTCTGTAGA AATCTGGGAC 
TGGGATCGAA CAACAAGGAA TGACTTCATG GGATCCCTTT CCTTTGGAGT TTCGGAGCTG 
ATGAAGATGC CGGCCAGTGG ATGGTACAAG TTGCTTAACC AAGAAGAAGG TGAGTACTAC 
AACGTACCCA TTCCGGAAGG GGACGAGGAA GGAAACATGG AACTCAGGCA GAAATTCGAG 
AAAGCCAAAC TTGGCCCTGC TGGCAACAAA GTCATCAGTC CCTCTGAAGA CAGGAAACAA 
CCTTCCAACA ACCTTGACCG AGTGAAACTC ACGGACTTCA ATTTCCTCAT GGTGTTGGGA 
AAGGGGAGTT TTGGAAAGGT GATGCTTGCC GACAGGAAGG GCACAGAAGA ACTGTATGCA 
ATCAAAATCC TGAAGAAGGA TGTGGTGATT CAGGATGATG ACGTGGAGTG CACCATGGTA 
GAAAAGCGAG TCTTGGCCCT GCTTGACAAA CCCCCGTTCT TGACGCAGCT GCACTCCTGC 
TTCCAGACAG TGGATCGGCT GTACTTCGTC ATGGAATATG TCAACGGTGG GGACCTCATG 
TACCACATTC AGCAAGTAGG AAAATTTAAG GAACCACAAG CAGTATTCTA TGCGGCAGAG 
ATTTCCATCG GATTGTTCTT TCTTCATAAA AGAGGAATCA TTTATAGGGA TCTGAAGTTA 
GATAACGTCA TGTTGGATTC AGAAGGACAT ATCAAAATTG CTGACTTTGG GATGTGCAAG 
GAACACATGA TGGATGGAGT CACGACCAGG ACCTTCTGTG GGACTCCAGA TTATATCGCC 
CCAGAGATAA TCGCTTATCA GCCGTATGGA AAATCTGTGG ACTGGTGGGC CTATGGCGTC 
CTGTTGTATG AAATGCTTGC CGGGCAGCCT CCATTTGATG GTGAAGATGA AGACGAGCTA 
TTTCAGTCTA TCATGGAGCA CAACGTTTCC TATCCAAAAT CCTTGTCCAA GGAGGCTGTT 
TCTGTCTGCA AAGGACTGAT GACCAAACAC CCAGCCAAGC GGCTGGGCTG TGGGCCTGAG 
GGGGAGAGGG ACGTGAGAGA GCATGCCTTC TTCCGGAGGA TCGACTGGGA AAAACTGGAG 
AACAGGGAGA TCCAGCCACC ATTCAAGCCC AAAGTGTGTG GCAAAGGAGC AGAGAACTTT 
GACAAGTTCT TCACACGAGG ACAGCCCGTC TTAACACCAC CTGATCAGCT GGTTATTGCT 
AACATAGACC AGTCTGATTT TGAAGGGTTC TCGTATGTCA ACCCCCAGTT TGTGCACCCC 
ATCTTACAGA GTGCAGTATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu86981
Clone ID Related Accession (Same CDS sequence) XM_024450830.1 , XM_017024839.1 , XM_017024840.1 , XM_024450829.1 , XM_017024838.1
Accession Version XM_017024838.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1761bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-05
Organism Homo sapiens(human)
Product protein kinase C alpha type isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGCTAAGTG TGTCATCCTG GGATTCAAAC GACCCCAGGA GCAAGCACAA GTTCAAAATC 
CACACTTACG GAAGCCCCAC CTTCTGCGAT CACTGTGGGT CACTGCTCTA TGGACTTATC 
CATCAAGGGA TGAAATGTGA CACCTGCGAT ATGAACGTTC ACAAGCAATG CGTCATCAAT 
GTCCCCAGCC TCTGCGGAAT GGATCACACT GAGAAGAGGG GGCGGATTTA CCTAAAGGCT 
GAGGTTGCTG ATGAAAAGCT CCATGTCACA GTACGAGATG CAAAAAATCT AATCCCTATG 
GATCCAAACG GGCTTTCAGA TCCTTATGTG AAGCTGAAAC TTATTCCTGA TCCCAAGAAT 
GAAAGCAAGC AAAAAACCAA AACCATCCGC TCCACACTAA ATCCGCAGTG GAATGAGTCC 
TTTACATTCA AATTGAAACC TTCAGACAAA GACCGACGAC TGTCTGTAGA AATCTGGGAC 
TGGGATCGAA CAACAAGGAA TGACTTCATG GGATCCCTTT CCTTTGGAGT TTCGGAGCTG 
ATGAAGATGC CGGCCAGTGG ATGGTACAAG TTGCTTAACC AAGAAGAAGG TGAGTACTAC 
AACGTACCCA TTCCGGAAGG GGACGAGGAA GGAAACATGG AACTCAGGCA GAAATTCGAG 
AAAGCCAAAC TTGGCCCTGC TGGCAACAAA GTCATCAGTC CCTCTGAAGA CAGGAAACAA 
CCTTCCAACA ACCTTGACCG AGTGAAACTC ACGGACTTCA ATTTCCTCAT GGTGTTGGGA 
AAGGGGAGTT TTGGAAAGGT GATGCTTGCC GACAGGAAGG GCACAGAAGA ACTGTATGCA 
ATCAAAATCC TGAAGAAGGA TGTGGTGATT CAGGATGATG ACGTGGAGTG CACCATGGTA 
GAAAAGCGAG TCTTGGCCCT GCTTGACAAA CCCCCGTTCT TGACGCAGCT GCACTCCTGC 
TTCCAGACAG TGGATCGGCT GTACTTCGTC ATGGAATATG TCAACGGTGG GGACCTCATG 
TACCACATTC AGCAAGTAGG AAAATTTAAG GAACCACAAG CAGTATTCTA TGCGGCAGAG 
ATTTCCATCG GATTGTTCTT TCTTCATAAA AGAGGAATCA TTTATAGGGA TCTGAAGTTA 
GATAACGTCA TGTTGGATTC AGAAGGACAT ATCAAAATTG CTGACTTTGG GATGTGCAAG 
GAACACATGA TGGATGGAGT CACGACCAGG ACCTTCTGTG GGACTCCAGA TTATATCGCC 
CCAGAGATAA TCGCTTATCA GCCGTATGGA AAATCTGTGG ACTGGTGGGC CTATGGCGTC 
CTGTTGTATG AAATGCTTGC CGGGCAGCCT CCATTTGATG GTGAAGATGA AGACGAGCTA 
TTTCAGTCTA TCATGGAGCA CAACGTTTCC TATCCAAAAT CCTTGTCCAA GGAGGCTGTT 
TCTGTCTGCA AAGGACTGAT GACCAAACAC CCAGCCAAGC GGCTGGGCTG TGGGCCTGAG 
GGGGAGAGGG ACGTGAGAGA GCATGCCTTC TTCCGGAGGA TCGACTGGGA AAAACTGGAG 
AACAGGGAGA TCCAGCCACC ATTCAAGCCC AAAGTGTGTG GCAAAGGAGC AGAGAACTTT 
GACAAGTTCT TCACACGAGG ACAGCCCGTC TTAACACCAC CTGATCAGCT GGTTATTGCT 
AACATAGACC AGTCTGATTT TGAAGGGTTC TCGTATGTCA ACCCCCAGTT TGTGCACCCC 
ATCTTACAGA GTGCAGTATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016880327.1
CDS230..1990
Translation

Target ORF information:

RefSeq Version XM_017024838.1
Organism Homo sapiens(human)
Definition Homo sapiens protein kinase C alpha (PRKCA), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017024838.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGCTAAGTG TGTCATCCTG GGATTCAAAC GACCCCAGGA GCAAGCACAA GTTCAAAATC 
CACACTTACG GAAGCCCCAC CTTCTGCGAT CACTGTGGGT CACTGCTCTA TGGACTTATC 
CATCAAGGGA TGAAATGTGA CACCTGCGAT ATGAACGTTC ACAAGCAATG CGTCATCAAT 
GTCCCCAGCC TCTGCGGAAT GGATCACACT GAGAAGAGGG GGCGGATTTA CCTAAAGGCT 
GAGGTTGCTG ATGAAAAGCT CCATGTCACA GTACGAGATG CAAAAAATCT AATCCCTATG 
GATCCAAACG GGCTTTCAGA TCCTTATGTG AAGCTGAAAC TTATTCCTGA TCCCAAGAAT 
GAAAGCAAGC AAAAAACCAA AACCATCCGC TCCACACTAA ATCCGCAGTG GAATGAGTCC 
TTTACATTCA AATTGAAACC TTCAGACAAA GACCGACGAC TGTCTGTAGA AATCTGGGAC 
TGGGATCGAA CAACAAGGAA TGACTTCATG GGATCCCTTT CCTTTGGAGT TTCGGAGCTG 
ATGAAGATGC CGGCCAGTGG ATGGTACAAG TTGCTTAACC AAGAAGAAGG TGAGTACTAC 
AACGTACCCA TTCCGGAAGG GGACGAGGAA GGAAACATGG AACTCAGGCA GAAATTCGAG 
AAAGCCAAAC TTGGCCCTGC TGGCAACAAA GTCATCAGTC CCTCTGAAGA CAGGAAACAA 
CCTTCCAACA ACCTTGACCG AGTGAAACTC ACGGACTTCA ATTTCCTCAT GGTGTTGGGA 
AAGGGGAGTT TTGGAAAGGT GATGCTTGCC GACAGGAAGG GCACAGAAGA ACTGTATGCA 
ATCAAAATCC TGAAGAAGGA TGTGGTGATT CAGGATGATG ACGTGGAGTG CACCATGGTA 
GAAAAGCGAG TCTTGGCCCT GCTTGACAAA CCCCCGTTCT TGACGCAGCT GCACTCCTGC 
TTCCAGACAG TGGATCGGCT GTACTTCGTC ATGGAATATG TCAACGGTGG GGACCTCATG 
TACCACATTC AGCAAGTAGG AAAATTTAAG GAACCACAAG CAGTATTCTA TGCGGCAGAG 
ATTTCCATCG GATTGTTCTT TCTTCATAAA AGAGGAATCA TTTATAGGGA TCTGAAGTTA 
GATAACGTCA TGTTGGATTC AGAAGGACAT ATCAAAATTG CTGACTTTGG GATGTGCAAG 
GAACACATGA TGGATGGAGT CACGACCAGG ACCTTCTGTG GGACTCCAGA TTATATCGCC 
CCAGAGATAA TCGCTTATCA GCCGTATGGA AAATCTGTGG ACTGGTGGGC CTATGGCGTC 
CTGTTGTATG AAATGCTTGC CGGGCAGCCT CCATTTGATG GTGAAGATGA AGACGAGCTA 
TTTCAGTCTA TCATGGAGCA CAACGTTTCC TATCCAAAAT CCTTGTCCAA GGAGGCTGTT 
TCTGTCTGCA AAGGACTGAT GACCAAACAC CCAGCCAAGC GGCTGGGCTG TGGGCCTGAG 
GGGGAGAGGG ACGTGAGAGA GCATGCCTTC TTCCGGAGGA TCGACTGGGA AAAACTGGAG 
AACAGGGAGA TCCAGCCACC ATTCAAGCCC AAAGTGTGTG GCAAAGGAGC AGAGAACTTT 
GACAAGTTCT TCACACGAGG ACAGCCCGTC TTAACACCAC CTGATCAGCT GGTTATTGCT 
AACATAGACC AGTCTGATTT TGAAGGGTTC TCGTATGTCA ACCCCCAGTT TGTGCACCCC 
ATCTTACAGA GTGCAGTATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed