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PRSS1 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol PRSS1
Entrez Gene ID 5644
Full Name protease, serine 1
Synonyms TRP1,TRY1,TRY4,TRYP1
General protein information
Preferred Names

protease, serine 1

Names

trypsin-1
TCR V beta 4.1
beta-trypsin
cationic trypsinogen
digestive zymogen
nonfunctional trypsin 1
trypsinogen 1
trypsinogen A

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

7

7q34

Summary This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is secreted by the pancreas and cleaved to its active form in the small intestine. It is active on peptide linkages involving the carboxyl group of lysine or arginine. Mutations in this gene are associated with hereditary pancreatitis. This gene and several other trypsinogen genes are localized to the T cell receptor beta locus on chromosome 7. [provided by RefSeq, Jul 2008].
Disorder MIM:

276000

Disorder Html: Trypsinogen deficiency (1); Pancreatitis, hereditary, 167800 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_002769.4 NP_002760.1 trypsin-1 preproprotein
XM_011516411.1 XP_011514713.1 trypsin-1 isoform X1



Pan troglodytes (chimpanzee) LOC742453 XP_003951210.1
Homo sapiens (human) PRSS1 NP_002760.1
Mus musculus (house mouse) Try10 NP_001034085.1
Pan troglodytes (chimpanzee) LOC100615987 XP_003318897.1
Drosophila melanogaster (fruit fly) CG10663 NP_001097592.1
Macaca mulatta (rhesus monkey) PRSS1 NP_001040586.1


Related articles in PubMed

Polymorphisms at PRSS1-PRSS2 and CLDN2-MORC4 loci associate with alcoholic and non-alcoholic chronic pancreatitis in a European replication study.
Derikx MH, Kovacs P, Scholz M, Masson E, Chen JM, Ruffert C, Lichtner P, Te Morsche RH, Cavestro GM, Férec C, Drenth JP, Witt H, Rosendahl J,
Gut64(9)1426-33(2015 Sep)

Gene conversion between cationic trypsinogen (PRSS1) and the pseudogene trypsinogen 6 (PRSS3P2) in patients with chronic pancreatitis.
Rygiel AM, Beer S, Simon P, Wertheim-Tysarowska K, Oracz G, Kucharzik T, Tysarowski A, Niepokój K, Kierkus J, Jurek M, Gawliński P, Poznański J, Bal J, Lerch MM, Sahin-Tóth M, Weiss FU
Human mutation36(3)350-6(2015 Mar)

PRSS1 and SPINK1 mutations in idiopathic chronic and recurrent acute pancreatitis.
Pelaez-Luna M, Robles-Diaz G, Canizales-Quinteros S, Tusié-Luna MT
World journal of gastroenterology20(33)11788-92(2014 Sep)

Tyrosine sulfation of human trypsin steers S2' subsite selectivity towards basic amino acids.
Szabó A, Salameh MA, Ludwig M, Radisky ES, Sahin-Tóth M
PloS one9(7)e102063(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Early-onset pancreatitis is associated strongly with PRSS1 or CTRC mutations and family history of pancreatitis.
Title: Early-Onset Acute Recurrent and Chronic Pancreatitis Is Associated with PRSS1 or CTRC Gene Mutations.

These findings suggest that IL-1beta plays a key role in trypsin upregulation and has a pathological role in multiple organ failure in influenza virus infection.
Title: IL-1β is a key cytokine that induces trypsin upregulation in the influenza virus-cytokine-trypsin cycle.

Three types of pathogenic PRSS1 variants was identified in 11 patients, including p.N29I (n=1), p.R122H (n=1), and p.G208A (n=9).
Title: PRSS1, SPINK1, CFTR, and CTRC Pathogenic Variants in Korean Patients With Idiopathic Pancreatitis.

Human anionic trypsinogen is controlled by CTRC in a manner that individual natural mutations are unlikely to increase stability enough to promote intra-pancreatic activation.
Title: Tighter Control by Chymotrypsin C (CTRC) Explains Lack of Association between Human Anionic Trypsinogen and Hereditary Pancreatitis.

PRSS1 variant p.L104P exhibits a variety of phenotypic changes that can increase risk for chronic pancreatitis. Mutation-induced misfolding and associated ER stress are the dominant effects that support a direct pathogenic role in chronic pancreatitis.
Title: Pathogenic cellular role of the p.L104P human cationic trypsinogen variant in chronic pancreatitis.

The following PRSS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRSS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu19842 NM_002769.4
Latest version!
Homo sapiens protease, serine 1 (PRSS1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$134.50-$188.30
$269.00
OHu59966 XM_011516411.1
Latest version!
Homo sapiens protease, serine 1 (PRSS1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00

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2-4 Clone 40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu19842
Clone ID Related Accession (Same CDS sequence) NM_002769.4
Accession Version NM_002769.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 744bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-11-18
Organism Homo sapiens(Human)
Product trypsin-1 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP321999.1, BC128226.1 and CA948204.1. This sequence is a reference standard in the RefSeqGene project. On Nov 3, 2010 this sequence version replaced NM_002769.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CA777765.1, BC128226.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGAATCCAC TCCTGATCCT TACCTTTGTG GCAGCTGCTC TTGCTGCCCC CTTTGATGAT 
GATGACAAGA TCGTTGGGGG CTACAACTGT GAGGAGAATT CTGTCCCCTA CCAGGTGTCC
CTGAATTCTG GCTACCACTT CTGTGGTGGC TCCCTCATCA ACGAACAGTG GGTGGTATCA
GCAGGCCACT GCTACAAGTC CCGCATCCAG GTGAGACTGG GAGAGCACAA CATCGAAGTC
CTGGAGGGGA ATGAGCAGTT CATCAATGCA GCCAAGATCA TCCGCCACCC CCAATACGAC
AGGAAGACTC TGAACAATGA CATCATGTTA ATCAAGCTCT CCTCACGTGC AGTAATCAAC
GCCCGCGTGT CCACCATCTC TCTGCCCACC GCCCCTCCAG CCACTGGCAC GAAGTGCCTC
ATCTCTGGCT GGGGCAACAC TGCGAGCTCT GGCGCCGACT ACCCAGACGA GCTGCAGTGC
CTGGATGCTC CTGTGCTGAG CCAGGCTAAG TGTGAAGCCT CCTACCCTGG AAAGATTACC
AGCAACATGT TCTGTGTGGG CTTCCTTGAG GGAGGCAAGG ATTCATGTCA GGGTGATTCT
GGTGGCCCTG TGGTCTGCAA TGGACAGCTC CAAGGAGTTG TCTCCTGGGG TGATGGCTGT
GCCCAGAAGA ACAAGCCTGG AGTCTACACC AAGGTCTACA ACTATGTGAA ATGGATTAAG
AACACCATAG CTGCCAATAG CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002760.1
CDS18..761
Misc Feature(1)84..734
Misc Feature(2)87..743
Misc Feature(3)87..89
Misc Feature(4)order(204..206,336..338,615..617)
Misc Feature(5)381..383
Misc Feature(6)477..479
Misc Feature(7)477..479
Misc Feature(8)order(597..599,660..662,666..668)
Misc Feature(9)597..599
Exon (1)1..57
Exon (2)58..217
Exon (3)218..471
Exon (4)472..608
Exon (5)609..817
Translation

Target ORF information:

RefSeq Version NM_002769.4
Organism Homo sapiens(Human)
Definition Homo sapiens protease, serine 1 (PRSS1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002769.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGAATCCAC TCCTGATCCT TACCTTTGTG GCAGCTGCTC TTGCTGCCCC CTTTGATGAT 
GATGACAAGA TCGTTGGGGG CTACAACTGT GAGGAGAATT CTGTCCCCTA CCAGGTGTCC
CTGAATTCTG GCTACCACTT CTGTGGTGGC TCCCTCATCA ACGAACAGTG GGTGGTATCA
GCAGGCCACT GCTACAAGTC CCGCATCCAG GTGAGACTGG GAGAGCACAA CATCGAAGTC
CTGGAGGGGA ATGAGCAGTT CATCAATGCA GCCAAGATCA TCCGCCACCC CCAATACGAC
AGGAAGACTC TGAACAATGA CATCATGTTA ATCAAGCTCT CCTCACGTGC AGTAATCAAC
GCCCGCGTGT CCACCATCTC TCTGCCCACC GCCCCTCCAG CCACTGGCAC GAAGTGCCTC
ATCTCTGGCT GGGGCAACAC TGCGAGCTCT GGCGCCGACT ACCCAGACGA GCTGCAGTGC
CTGGATGCTC CTGTGCTGAG CCAGGCTAAG TGTGAAGCCT CCTACCCTGG AAAGATTACC
AGCAACATGT TCTGTGTGGG CTTCCTTGAG GGAGGCAAGG ATTCATGTCA GGGTGATTCT
GGTGGCCCTG TGGTCTGCAA TGGACAGCTC CAAGGAGTTG TCTCCTGGGG TGATGGCTGT
GCCCAGAAGA ACAAGCCTGG AGTCTACACC AAGGTCTACA ACTATGTGAA ATGGATTAAG
AACACCATAG CTGCCAATAG CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59966
Clone ID Related Accession (Same CDS sequence) XM_011516411.1
Accession Version XM_011516411.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1419bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product trypsin-1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCTGAGTC TTCTGCTCCT TCTCCTGGGA CTAGGCTCTG TGTTCAGTGC TGTCATCTCT 
CAAAAGCCAA GCAGGGATAT CTGTCAACGT GGAACCTCCC TGACGATCCA GTGTCAAGTC
GATAGCCAAG TCACCATGAT GTTCTGGTAC CGTCAGCAAC CTGGACAGAG CCTGACACTG
ATCGCAACTG CAAATCAGGG CTCTGAGGCC ACATATGAGA GTGGATTTGT CATTGACAAG
TTTCCCATCA GCCGCCCAAA CCTAACATTC TCAACTCTGA CTGTGAGCAA CATGAGCCCT
GAAGACAGCA GCATATATCT CTGCAGCGTT GAAGACACAG TGCGGGGCAC AGATCAAAGA
TCTGAGCAAG AACCTCAGCT CTCTCCTACC CAGCTCCTCT CACACGAGCC TGAAGGCCCT
GCCAAGTATC CTTGCAGGAA ACAGCCGGTC TCGCTCTCTG CTCTCAGAAG GAAAGTTTCC
TTATCACCTG TGAATCACAA ACCCAGAGAG TGGCCAAACA TAGCCAGGCT GATGCAAGAC
CCTGGGAAGA GGAAAGCTGC AGGTGTGTTT GTGCTGGGAG GAGTGGTGAC CCTCACCTCA
CAGTCACCTC CTCTCTGGAT CCTCGTGAGG TATAAAGACG AGTCCTCCAC CACCAGTCAG
GCACACTCTA CCACCATGAA TCCACTCCTG ATCCTTACCT TTGTGGCAGC TGCTCTTGCT
GCCCCCTTTG ATGATGATGA CAAGATCGTT GGGGGCTACA ACTGTGAGGA GAATTCTGTC
CCCTACCAGG TGTCCCTGAA TTCTGGCTAC CACTTCTGTG GTGGCTCCCT CATCAACGAA
CAGTGGGTGG TATCAGCAGG CCACTGCTAC AAGTCCCGCA TCCAGGTGAG ACTGGGAGAG
CACAACATCG AAGTCCTGGA GGGGAATGAG CAGTTCATCA ATGCAGCCAA GATCATCCGC
CACCCCCAAT ACGACAGGAA GACTCTGAAC AATGACATCA TGTTAATCAA GCTCTCCTCA
CGTGCAGTAA TCAACGCCCG CGTGTCCACC ATCTCTCTGC CCACCGCCCC TCCAGCCACT
GGCACGAAGT GCCTCATCTC TGGCTGGGGC AACACTGCGA GCTCTGGCGC CGACTACCCA
GACGAGCTGC AGTGCCTGGA TGCTCCTGTG CTGAGCCAGG CTAAGTGTGA AGCCTCCTAC
CCTGGAAAGA TTACCAGCAA CATGTTCTGT GTGGGCTTCC TTGAGGGAGG CAAGGATTCA
TGTCAGGGTG ATTCTGGTGG CCCTGTGGTC TGCAATGGAC AGCTCCAAGG AGTTGTCTCC
TGGGGTGATG GCTGTGCCCA GAAGAACAAG CCTGGAGTCT ACACCAAGGT CTACAACTAT
GTGAAATGGA TTAAGAACAC CATAGCTGCC AATAGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011514713.1
CDS39..1457
Misc Feature(1)93..395
Misc Feature(2)123..377
Misc Feature(3)780..1430
Misc Feature(4)783..1439
Misc Feature(5)783..785
Misc Feature(6)order(900..902,1032..1034,1311..1313)
Misc Feature(7)order(1293..1295,1356..1358,1362..1364)
Translation

Target ORF information:

RefSeq Version XM_011516411.1
Organism Homo sapiens(Human)
Definition Homo sapiens protease, serine 1 (PRSS1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011516411.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCTGAGTC TTCTGCTCCT TCTCCTGGGA CTAGGCTCTG TGTTCAGTGC TGTCATCTCT 
CAAAAGCCAA GCAGGGATAT CTGTCAACGT GGAACCTCCC TGACGATCCA GTGTCAAGTC
GATAGCCAAG TCACCATGAT GTTCTGGTAC CGTCAGCAAC CTGGACAGAG CCTGACACTG
ATCGCAACTG CAAATCAGGG CTCTGAGGCC ACATATGAGA GTGGATTTGT CATTGACAAG
TTTCCCATCA GCCGCCCAAA CCTAACATTC TCAACTCTGA CTGTGAGCAA CATGAGCCCT
GAAGACAGCA GCATATATCT CTGCAGCGTT GAAGACACAG TGCGGGGCAC AGATCAAAGA
TCTGAGCAAG AACCTCAGCT CTCTCCTACC CAGCTCCTCT CACACGAGCC TGAAGGCCCT
GCCAAGTATC CTTGCAGGAA ACAGCCGGTC TCGCTCTCTG CTCTCAGAAG GAAAGTTTCC
TTATCACCTG TGAATCACAA ACCCAGAGAG TGGCCAAACA TAGCCAGGCT GATGCAAGAC
CCTGGGAAGA GGAAAGCTGC AGGTGTGTTT GTGCTGGGAG GAGTGGTGAC CCTCACCTCA
CAGTCACCTC CTCTCTGGAT CCTCGTGAGG TATAAAGACG AGTCCTCCAC CACCAGTCAG
GCACACTCTA CCACCATGAA TCCACTCCTG ATCCTTACCT TTGTGGCAGC TGCTCTTGCT
GCCCCCTTTG ATGATGATGA CAAGATCGTT GGGGGCTACA ACTGTGAGGA GAATTCTGTC
CCCTACCAGG TGTCCCTGAA TTCTGGCTAC CACTTCTGTG GTGGCTCCCT CATCAACGAA
CAGTGGGTGG TATCAGCAGG CCACTGCTAC AAGTCCCGCA TCCAGGTGAG ACTGGGAGAG
CACAACATCG AAGTCCTGGA GGGGAATGAG CAGTTCATCA ATGCAGCCAA GATCATCCGC
CACCCCCAAT ACGACAGGAA GACTCTGAAC AATGACATCA TGTTAATCAA GCTCTCCTCA
CGTGCAGTAA TCAACGCCCG CGTGTCCACC ATCTCTCTGC CCACCGCCCC TCCAGCCACT
GGCACGAAGT GCCTCATCTC TGGCTGGGGC AACACTGCGA GCTCTGGCGC CGACTACCCA
GACGAGCTGC AGTGCCTGGA TGCTCCTGTG CTGAGCCAGG CTAAGTGTGA AGCCTCCTAC
CCTGGAAAGA TTACCAGCAA CATGTTCTGT GTGGGCTTCC TTGAGGGAGG CAAGGATTCA
TGTCAGGGTG ATTCTGGTGG CCCTGTGGTC TGCAATGGAC AGCTCCAAGG AGTTGTCTCC
TGGGGTGATG GCTGTGCCCA GAAGAACAAG CCTGGAGTCT ACACCAAGGT CTACAACTAT
GTGAAATGGA TTAAGAACAC CATAGCTGCC AATAGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Polymorphisms at PRSS1-PRSS2 and CLDN2-MORC4 loci associate with alcoholic and non-alcoholic chronic pancreatitis in a European replication study.
Gut64(9)1426-33(2015 Sep)
Derikx MH,Kovacs P,Scholz M,Masson E,Chen JM,Ruffert C,Lichtner P,Te Morsche RH,Cavestro GM,Férec C,Drenth JP,Witt H,Rosendahl J,


book

Gene conversion between cationic trypsinogen (PRSS1) and the pseudogene trypsinogen 6 (PRSS3P2) in patients with chronic pancreatitis.
Human mutation36(3)350-6(2015 Mar)
Rygiel AM,Beer S,Simon P,Wertheim-Tysarowska K,Oracz G,Kucharzik T,Tysarowski A,Niepokój K,Kierkus J,Jurek M,Gawliński P,Poznański J,Bal J,Lerch MM,Sahin-Tóth M,Weiss FU


book

PRSS1 and SPINK1 mutations in idiopathic chronic and recurrent acute pancreatitis.
World journal of gastroenterology20(33)11788-92(2014 Sep)
Pelaez-Luna M,Robles-Diaz G,Canizales-Quinteros S,Tusié-Luna MT


book

Tyrosine sulfation of human trypsin steers S2' subsite selectivity towards basic amino acids.
PloS one9(7)e102063(2014)
Szabó A,Salameh MA,Ludwig M,Radisky ES,Sahin-Tóth M


 
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