PTEN cDNA ORF clone, Homo sapiens(human)

The following PTEN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PTEN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu27709 NM_000314.8
Latest version!
Homo sapiens phosphatase and tensin homolog (PTEN), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu59999 NM_001304717.5
Latest version!
Homo sapiens phosphatase and tensin homolog (PTEN), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu60002 NM_001304718.2
Latest version!
Homo sapiens phosphatase and tensin homolog (PTEN), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$99.50-$139.30
$199.00
View Old Accession Versions >>
OHu59999 NM_001304717.2 Homo sapiens phosphatase and tensin homolog (PTEN), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu27709 NM_000314.7 Homo sapiens phosphatase and tensin homolog (PTEN), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu60002 NM_001304718.1 Homo sapiens phosphatase and tensin homolog (PTEN), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$99.50-$139.30
$199.00
OHu27709 NM_000314.6 Homo sapiens phosphatase and tensin homolog (PTEN), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu27709
    Clone ID Related Accession (Same CDS sequence) NM_000314.8 , NM_000314.7 , NM_000314.6
    Accession Version NM_000314.8 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1212bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN isoform PTEN
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC063965.8. On Aug 31, 2019 this sequence version replaced NM_000314.7. Transcript Variant: This variant (1) encodes multiple isoforms due to the use of alternative translation initiation codons. The longest isoform (PTEN-L, PMID:23744781; also known as PTENalpha, PMID: 24768297) is derived from the use of an upstream non-AUG (CUG) start codon, while two shorter isoforms are derived from downstream AUG start codons. The most abundant isoform (PTEN), which is derived from the use of the 5'-most AUG start codon, is represented in this RefSeq. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U92436.1, BC005821.2 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:24768297) MANE Ensembl match :: ENST00000371953.8/ ENSP00000361021.3 RefSeq Select criteria :: based on expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGACAGCCA TCATCAAAGA GATCGTTAGC AGAAACAAAA GGAGATATCA AGAGGATGGA 
    TTCGACTTAG ACTTGACCTA TATTTATCCA AACATTATTG CTATGGGATT TCCTGCAGAA
    AGACTTGAAG GCGTATACAG GAACAATATT GATGATGTAG TAAGGTTTTT GGATTCAAAG
    CATAAAAACC ATTACAAGAT ATACAATCTT TGTGCTGAAA GACATTATGA CACCGCCAAA
    TTTAATTGCA GAGTTGCACA ATATCCTTTT GAAGACCATA ACCCACCACA GCTAGAACTT
    ATCAAACCCT TTTGTGAAGA TCTTGACCAA TGGCTAAGTG AAGATGACAA TCATGTTGCA
    GCAATTCACT GTAAAGCTGG AAAGGGACGA ACTGGTGTAA TGATATGTGC ATATTTATTA
    CATCGGGGCA AATTTTTAAA GGCACAAGAG GCCCTAGATT TCTATGGGGA AGTAAGGACC
    AGAGACAAAA AGGGAGTAAC TATTCCCAGT CAGAGGCGCT ATGTGTATTA TTATAGCTAC
    CTGTTAAAGA ATCATCTGGA TTATAGACCA GTGGCACTGT TGTTTCACAA GATGATGTTT
    GAAACTATTC CAATGTTCAG TGGCGGAACT TGCAATCCTC AGTTTGTGGT CTGCCAGCTA
    AAGGTGAAGA TATATTCCTC CAATTCAGGA CCCACACGAC GGGAAGACAA GTTCATGTAC
    TTTGAGTTCC CTCAGCCGTT ACCTGTGTGT GGTGATATCA AAGTAGAGTT CTTCCACAAA
    CAGAACAAGA TGCTAAAAAA GGACAAAATG TTTCACTTTT GGGTAAATAC ATTCTTCATA
    CCAGGACCAG AGGAAACCTC AGAAAAAGTA GAAAATGGAA GTCTATGTGA TCAAGAAATC
    GATAGCATTT GCAGTATAGA GCGTGCAGAT AATGACAAGG AATATCTAGT ACTTACTTTA
    ACAAAAAATG ATCTTGACAA AGCAAATAAA GACAAAGCCA ACCGATACTT TTCTCCAAAT
    TTTAAGGTGA AGCTGTACTT CACAAAAACA GTAGAGGAGC CGTCAAATCC AGAGGCTAGC
    AGTTCAACTT CTGTAACACC AGATGTTAGT GACAATGAAC CTGATCATTA TAGATATTCT
    GACACCACTG ACTCTGATCC AGAGAATGAA CCTTTTGATG AAGATCAGCA TACACAAATT
    ACAAAAGTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000305.3
    CDS846..2057
    Translation

    Target ORF information:

    RefSeq Version NM_000314.8
    Organism Homo sapiens(human)
    Definition Homo sapiens phosphatase and tensin homolog (PTEN), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000314.8

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGACAGCCA TCATCAAAGA GATCGTTAGC AGAAACAAAA GGAGATATCA AGAGGATGGA 
    TTCGACTTAG ACTTGACCTA TATTTATCCA AACATTATTG CTATGGGATT TCCTGCAGAA
    AGACTTGAAG GCGTATACAG GAACAATATT GATGATGTAG TAAGGTTTTT GGATTCAAAG
    CATAAAAACC ATTACAAGAT ATACAATCTT TGTGCTGAAA GACATTATGA CACCGCCAAA
    TTTAATTGCA GAGTTGCACA ATATCCTTTT GAAGACCATA ACCCACCACA GCTAGAACTT
    ATCAAACCCT TTTGTGAAGA TCTTGACCAA TGGCTAAGTG AAGATGACAA TCATGTTGCA
    GCAATTCACT GTAAAGCTGG AAAGGGACGA ACTGGTGTAA TGATATGTGC ATATTTATTA
    CATCGGGGCA AATTTTTAAA GGCACAAGAG GCCCTAGATT TCTATGGGGA AGTAAGGACC
    AGAGACAAAA AGGGAGTAAC TATTCCCAGT CAGAGGCGCT ATGTGTATTA TTATAGCTAC
    CTGTTAAAGA ATCATCTGGA TTATAGACCA GTGGCACTGT TGTTTCACAA GATGATGTTT
    GAAACTATTC CAATGTTCAG TGGCGGAACT TGCAATCCTC AGTTTGTGGT CTGCCAGCTA
    AAGGTGAAGA TATATTCCTC CAATTCAGGA CCCACACGAC GGGAAGACAA GTTCATGTAC
    TTTGAGTTCC CTCAGCCGTT ACCTGTGTGT GGTGATATCA AAGTAGAGTT CTTCCACAAA
    CAGAACAAGA TGCTAAAAAA GGACAAAATG TTTCACTTTT GGGTAAATAC ATTCTTCATA
    CCAGGACCAG AGGAAACCTC AGAAAAAGTA GAAAATGGAA GTCTATGTGA TCAAGAAATC
    GATAGCATTT GCAGTATAGA GCGTGCAGAT AATGACAAGG AATATCTAGT ACTTACTTTA
    ACAAAAAATG ATCTTGACAA AGCAAATAAA GACAAAGCCA ACCGATACTT TTCTCCAAAT
    TTTAAGGTGA AGCTGTACTT CACAAAAACA GTAGAGGAGC CGTCAAATCC AGAGGCTAGC
    AGTTCAACTT CTGTAACACC AGATGTTAGT GACAATGAAC CTGATCATTA TAGATATTCT
    GACACCACTG ACTCTGATCC AGAGAATGAA CCTTTTGATG AAGATCAGCA TACACAAATT
    ACAAAAGTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27709
    Clone ID Related Accession (Same CDS sequence) NM_000314.8 , NM_000314.7 , NM_000314.6
    Accession Version NM_000314.7 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1212bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-01
    Organism Homo sapiens(human)
    Product phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN isoform PTEN
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U92436.1, BC005821.2, AC063965.8 and AA836562.1. On Nov 21, 2018 this sequence version replaced NM_000314.6. Transcript Variant: This variant (1) encodes multiple isoforms due to the use of alternative translation initiation codons. The longest isoform (PTEN-L, PMID:23744781; also known as PTENalpha, PMID: 24768297) is derived from the use of an upstream non-AUG (CUG) start codon, while two shorter isoforms are derived from downstream AUG start codons. The most abundant isoform (PTEN), which is derived from the use of the 5'-most AUG start codon, is represented in this RefSeq. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.107470.1, SRR1660807.191185.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:24768297) RefSeq Select criteria :: based on expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGACAGCCA TCATCAAAGA GATCGTTAGC AGAAACAAAA GGAGATATCA AGAGGATGGA 
    TTCGACTTAG ACTTGACCTA TATTTATCCA AACATTATTG CTATGGGATT TCCTGCAGAA
    AGACTTGAAG GCGTATACAG GAACAATATT GATGATGTAG TAAGGTTTTT GGATTCAAAG
    CATAAAAACC ATTACAAGAT ATACAATCTT TGTGCTGAAA GACATTATGA CACCGCCAAA
    TTTAATTGCA GAGTTGCACA ATATCCTTTT GAAGACCATA ACCCACCACA GCTAGAACTT
    ATCAAACCCT TTTGTGAAGA TCTTGACCAA TGGCTAAGTG AAGATGACAA TCATGTTGCA
    GCAATTCACT GTAAAGCTGG AAAGGGACGA ACTGGTGTAA TGATATGTGC ATATTTATTA
    CATCGGGGCA AATTTTTAAA GGCACAAGAG GCCCTAGATT TCTATGGGGA AGTAAGGACC
    AGAGACAAAA AGGGAGTAAC TATTCCCAGT CAGAGGCGCT ATGTGTATTA TTATAGCTAC
    CTGTTAAAGA ATCATCTGGA TTATAGACCA GTGGCACTGT TGTTTCACAA GATGATGTTT
    GAAACTATTC CAATGTTCAG TGGCGGAACT TGCAATCCTC AGTTTGTGGT CTGCCAGCTA
    AAGGTGAAGA TATATTCCTC CAATTCAGGA CCCACACGAC GGGAAGACAA GTTCATGTAC
    TTTGAGTTCC CTCAGCCGTT ACCTGTGTGT GGTGATATCA AAGTAGAGTT CTTCCACAAA
    CAGAACAAGA TGCTAAAAAA GGACAAAATG TTTCACTTTT GGGTAAATAC ATTCTTCATA
    CCAGGACCAG AGGAAACCTC AGAAAAAGTA GAAAATGGAA GTCTATGTGA TCAAGAAATC
    GATAGCATTT GCAGTATAGA GCGTGCAGAT AATGACAAGG AATATCTAGT ACTTACTTTA
    ACAAAAAATG ATCTTGACAA AGCAAATAAA GACAAAGCCA ACCGATACTT TTCTCCAAAT
    TTTAAGGTGA AGCTGTACTT CACAAAAACA GTAGAGGAGC CGTCAAATCC AGAGGCTAGC
    AGTTCAACTT CTGTAACACC AGATGTTAGT GACAATGAAC CTGATCATTA TAGATATTCT
    GACACCACTG ACTCTGATCC AGAGAATGAA CCTTTTGATG AAGATCAGCA TACACAAATT
    ACAAAAGTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000305.3
    CDS845..2056
    Translation

    Target ORF information:

    RefSeq Version NM_000314.7
    Organism Homo sapiens(human)
    Definition Homo sapiens phosphatase and tensin homolog (PTEN), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000314.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGACAGCCA TCATCAAAGA GATCGTTAGC AGAAACAAAA GGAGATATCA AGAGGATGGA 
    TTCGACTTAG ACTTGACCTA TATTTATCCA AACATTATTG CTATGGGATT TCCTGCAGAA
    AGACTTGAAG GCGTATACAG GAACAATATT GATGATGTAG TAAGGTTTTT GGATTCAAAG
    CATAAAAACC ATTACAAGAT ATACAATCTT TGTGCTGAAA GACATTATGA CACCGCCAAA
    TTTAATTGCA GAGTTGCACA ATATCCTTTT GAAGACCATA ACCCACCACA GCTAGAACTT
    ATCAAACCCT TTTGTGAAGA TCTTGACCAA TGGCTAAGTG AAGATGACAA TCATGTTGCA
    GCAATTCACT GTAAAGCTGG AAAGGGACGA ACTGGTGTAA TGATATGTGC ATATTTATTA
    CATCGGGGCA AATTTTTAAA GGCACAAGAG GCCCTAGATT TCTATGGGGA AGTAAGGACC
    AGAGACAAAA AGGGAGTAAC TATTCCCAGT CAGAGGCGCT ATGTGTATTA TTATAGCTAC
    CTGTTAAAGA ATCATCTGGA TTATAGACCA GTGGCACTGT TGTTTCACAA GATGATGTTT
    GAAACTATTC CAATGTTCAG TGGCGGAACT TGCAATCCTC AGTTTGTGGT CTGCCAGCTA
    AAGGTGAAGA TATATTCCTC CAATTCAGGA CCCACACGAC GGGAAGACAA GTTCATGTAC
    TTTGAGTTCC CTCAGCCGTT ACCTGTGTGT GGTGATATCA AAGTAGAGTT CTTCCACAAA
    CAGAACAAGA TGCTAAAAAA GGACAAAATG TTTCACTTTT GGGTAAATAC ATTCTTCATA
    CCAGGACCAG AGGAAACCTC AGAAAAAGTA GAAAATGGAA GTCTATGTGA TCAAGAAATC
    GATAGCATTT GCAGTATAGA GCGTGCAGAT AATGACAAGG AATATCTAGT ACTTACTTTA
    ACAAAAAATG ATCTTGACAA AGCAAATAAA GACAAAGCCA ACCGATACTT TTCTCCAAAT
    TTTAAGGTGA AGCTGTACTT CACAAAAACA GTAGAGGAGC CGTCAAATCC AGAGGCTAGC
    AGTTCAACTT CTGTAACACC AGATGTTAGT GACAATGAAC CTGATCATTA TAGATATTCT
    GACACCACTG ACTCTGATCC AGAGAATGAA CCTTTTGATG AAGATCAGCA TACACAAATT
    ACAAAAGTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27709
    Clone ID Related Accession (Same CDS sequence) NM_000314.8 , NM_000314.7 , NM_000314.6
    Accession Version NM_000314.6 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1212bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-31
    Organism Homo sapiens(human)
    Product phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN isoform PTEN
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U92436.1, AC063965.8, BC005821.2 and AA836562.1. On Mar 25, 2015 this sequence version replaced NM_000314.5. Transcript Variant: This variant (1) encodes multiple isoforms due to the use of alternative translation initiation codons. The longest isoform (PTEN-L, PMID:23744781; also known as PTENalpha, PMID: 24768297) is derived from the use of an upstream non-AUG (CUG) start codon, while two shorter isoforms are derived from downstream AUG start codons. The most abundant isoform (PTEN), which is derived from the use of the 5'-most AUG start codon, is represented in this RefSeq. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U92436.1, SRR1660807.191185.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:24768297) ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGACAGCCA TCATCAAAGA GATCGTTAGC AGAAACAAAA GGAGATATCA AGAGGATGGA 
    TTCGACTTAG ACTTGACCTA TATTTATCCA AACATTATTG CTATGGGATT TCCTGCAGAA
    AGACTTGAAG GCGTATACAG GAACAATATT GATGATGTAG TAAGGTTTTT GGATTCAAAG
    CATAAAAACC ATTACAAGAT ATACAATCTT TGTGCTGAAA GACATTATGA CACCGCCAAA
    TTTAATTGCA GAGTTGCACA ATATCCTTTT GAAGACCATA ACCCACCACA GCTAGAACTT
    ATCAAACCCT TTTGTGAAGA TCTTGACCAA TGGCTAAGTG AAGATGACAA TCATGTTGCA
    GCAATTCACT GTAAAGCTGG AAAGGGACGA ACTGGTGTAA TGATATGTGC ATATTTATTA
    CATCGGGGCA AATTTTTAAA GGCACAAGAG GCCCTAGATT TCTATGGGGA AGTAAGGACC
    AGAGACAAAA AGGGAGTAAC TATTCCCAGT CAGAGGCGCT ATGTGTATTA TTATAGCTAC
    CTGTTAAAGA ATCATCTGGA TTATAGACCA GTGGCACTGT TGTTTCACAA GATGATGTTT
    GAAACTATTC CAATGTTCAG TGGCGGAACT TGCAATCCTC AGTTTGTGGT CTGCCAGCTA
    AAGGTGAAGA TATATTCCTC CAATTCAGGA CCCACACGAC GGGAAGACAA GTTCATGTAC
    TTTGAGTTCC CTCAGCCGTT ACCTGTGTGT GGTGATATCA AAGTAGAGTT CTTCCACAAA
    CAGAACAAGA TGCTAAAAAA GGACAAAATG TTTCACTTTT GGGTAAATAC ATTCTTCATA
    CCAGGACCAG AGGAAACCTC AGAAAAAGTA GAAAATGGAA GTCTATGTGA TCAAGAAATC
    GATAGCATTT GCAGTATAGA GCGTGCAGAT AATGACAAGG AATATCTAGT ACTTACTTTA
    ACAAAAAATG ATCTTGACAA AGCAAATAAA GACAAAGCCA ACCGATACTT TTCTCCAAAT
    TTTAAGGTGA AGCTGTACTT CACAAAAACA GTAGAGGAGC CGTCAAATCC AGAGGCTAGC
    AGTTCAACTT CTGTAACACC AGATGTTAGT GACAATGAAC CTGATCATTA TAGATATTCT
    GACACCACTG ACTCTGATCC AGAGAATGAA CCTTTTGATG AAGATCAGCA TACACAAATT
    ACAAAAGTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000305.3
    CDS1032..2243
    Translation

    Target ORF information:

    RefSeq Version NM_000314.6
    Organism Homo sapiens(human)
    Definition Homo sapiens phosphatase and tensin homolog (PTEN), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000314.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGACAGCCA TCATCAAAGA GATCGTTAGC AGAAACAAAA GGAGATATCA AGAGGATGGA 
    TTCGACTTAG ACTTGACCTA TATTTATCCA AACATTATTG CTATGGGATT TCCTGCAGAA
    AGACTTGAAG GCGTATACAG GAACAATATT GATGATGTAG TAAGGTTTTT GGATTCAAAG
    CATAAAAACC ATTACAAGAT ATACAATCTT TGTGCTGAAA GACATTATGA CACCGCCAAA
    TTTAATTGCA GAGTTGCACA ATATCCTTTT GAAGACCATA ACCCACCACA GCTAGAACTT
    ATCAAACCCT TTTGTGAAGA TCTTGACCAA TGGCTAAGTG AAGATGACAA TCATGTTGCA
    GCAATTCACT GTAAAGCTGG AAAGGGACGA ACTGGTGTAA TGATATGTGC ATATTTATTA
    CATCGGGGCA AATTTTTAAA GGCACAAGAG GCCCTAGATT TCTATGGGGA AGTAAGGACC
    AGAGACAAAA AGGGAGTAAC TATTCCCAGT CAGAGGCGCT ATGTGTATTA TTATAGCTAC
    CTGTTAAAGA ATCATCTGGA TTATAGACCA GTGGCACTGT TGTTTCACAA GATGATGTTT
    GAAACTATTC CAATGTTCAG TGGCGGAACT TGCAATCCTC AGTTTGTGGT CTGCCAGCTA
    AAGGTGAAGA TATATTCCTC CAATTCAGGA CCCACACGAC GGGAAGACAA GTTCATGTAC
    TTTGAGTTCC CTCAGCCGTT ACCTGTGTGT GGTGATATCA AAGTAGAGTT CTTCCACAAA
    CAGAACAAGA TGCTAAAAAA GGACAAAATG TTTCACTTTT GGGTAAATAC ATTCTTCATA
    CCAGGACCAG AGGAAACCTC AGAAAAAGTA GAAAATGGAA GTCTATGTGA TCAAGAAATC
    GATAGCATTT GCAGTATAGA GCGTGCAGAT AATGACAAGG AATATCTAGT ACTTACTTTA
    ACAAAAAATG ATCTTGACAA AGCAAATAAA GACAAAGCCA ACCGATACTT TTCTCCAAAT
    TTTAAGGTGA AGCTGTACTT CACAAAAACA GTAGAGGAGC CGTCAAATCC AGAGGCTAGC
    AGTTCAACTT CTGTAACACC AGATGTTAGT GACAATGAAC CTGATCATTA TAGATATTCT
    GACACCACTG ACTCTGATCC AGAGAATGAA CCTTTTGATG AAGATCAGCA TACACAAATT
    ACAAAAGTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu59999
    Clone ID Related Accession (Same CDS sequence) NM_001304717.2 , NM_001304717.5
    Accession Version NM_001304717.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1731bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-31
    Organism Homo sapiens(human)
    Product phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN isoform PTEN-L
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U92436.1, AC063965.8, BC005821.2 and AA836562.1. On Apr 1, 2015 this sequence version replaced NM_001304717.1. Transcript Variant: This variant (1) encodes multiple isoforms due to the use of alternative translation initiation codons. The longest isoform (PTEN-L, PMID:23744781; also known as PTENalpha, PMID: 24768297) is derived from the use of an upstream non-AUG (CUG) start codon, while two shorter isoforms are derived from downstream AUG start codons. PTEN-L, initiates with a Leucine, rather than a Methionine, is thought to be preferentially associated with the mitochondrial inner membrane (PMID: 24768297), and is represented in this RefSeq. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U92436.1, SRR1660807.191185.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 23744781, 24768297 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    CTGGAGCGGG GGGGAGAAGC GGCGGCGGCG GCGGCCGCGG CGGCTGCAGC TCCAGGGAGG 
    GGGTCTGAGT CGCCTGTCAC CATTTCCAGG GCTGGGAACG CCGGAGAGTT GGTCTCTCCC
    CTTCTACTGC CTCCAACACG GCGGCGGCGG CGGCGGCACA TCCAGGGACC CGGGCCGGTT
    TTAAACCTCC CGTCCGCCGC CGCCGCACCC CCCGTGGCCC GGGCTCCGGA GGCCGCCGGC
    GGAGGCAGCC GTTCGGAGGA TTATTCGTCT TCTCCCCATT CCGCTGCCGC CGCTGCCAGG
    CCTCTGGCTG CTGAGGAGAA GCAGGCCCAG TCGCTGCAAC CATCCAGCAG CCGCCGCAGC
    AGCCATTACC CGGCTGCGGT CCAGAGCCAA GCGGCGGCAG AGCGAGGGGC ATCAGCTACC
    GCCAAGTCCA GAGCCATTTC CATCCTGCAG AAGAAGCCCC GCCACCAGCA GCTTCTGCCA
    TCTCTCTCCT CCTTTTTCTT CAGCCACAGG CTCCCAGACA TGACAGCCAT CATCAAAGAG
    ATCGTTAGCA GAAACAAAAG GAGATATCAA GAGGATGGAT TCGACTTAGA CTTGACCTAT
    ATTTATCCAA ACATTATTGC TATGGGATTT CCTGCAGAAA GACTTGAAGG CGTATACAGG
    AACAATATTG ATGATGTAGT AAGGTTTTTG GATTCAAAGC ATAAAAACCA TTACAAGATA
    TACAATCTTT GTGCTGAAAG ACATTATGAC ACCGCCAAAT TTAATTGCAG AGTTGCACAA
    TATCCTTTTG AAGACCATAA CCCACCACAG CTAGAACTTA TCAAACCCTT TTGTGAAGAT
    CTTGACCAAT GGCTAAGTGA AGATGACAAT CATGTTGCAG CAATTCACTG TAAAGCTGGA
    AAGGGACGAA CTGGTGTAAT GATATGTGCA TATTTATTAC ATCGGGGCAA ATTTTTAAAG
    GCACAAGAGG CCCTAGATTT CTATGGGGAA GTAAGGACCA GAGACAAAAA GGGAGTAACT
    ATTCCCAGTC AGAGGCGCTA TGTGTATTAT TATAGCTACC TGTTAAAGAA TCATCTGGAT
    TATAGACCAG TGGCACTGTT GTTTCACAAG ATGATGTTTG AAACTATTCC AATGTTCAGT
    GGCGGAACTT GCAATCCTCA GTTTGTGGTC TGCCAGCTAA AGGTGAAGAT ATATTCCTCC
    AATTCAGGAC CCACACGACG GGAAGACAAG TTCATGTACT TTGAGTTCCC TCAGCCGTTA
    CCTGTGTGTG GTGATATCAA AGTAGAGTTC TTCCACAAAC AGAACAAGAT GCTAAAAAAG
    GACAAAATGT TTCACTTTTG GGTAAATACA TTCTTCATAC CAGGACCAGA GGAAACCTCA
    GAAAAAGTAG AAAATGGAAG TCTATGTGAT CAAGAAATCG ATAGCATTTG CAGTATAGAG
    CGTGCAGATA ATGACAAGGA ATATCTAGTA CTTACTTTAA CAAAAAATGA TCTTGACAAA
    GCAAATAAAG ACAAAGCCAA CCGATACTTT TCTCCAAATT TTAAGGTGAA GCTGTACTTC
    ACAAAAACAG TAGAGGAGCC GTCAAATCCA GAGGCTAGCA GTTCAACTTC TGTAACACCA
    GATGTTAGTG ACAATGAACC TGATCATTAT AGATATTCTG ACACCACTGA CTCTGATCCA
    GAGAATGAAC CTTTTGATGA AGATCAGCAT ACACAAATTA CAAAAGTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001291646.2
    CDS513..2243
    Translation

    Target ORF information:

    RefSeq Version NM_001304717.2
    Organism Homo sapiens(human)
    Definition Homo sapiens phosphatase and tensin homolog (PTEN), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001304717.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    CTGGAGCGGG GGGGAGAAGC GGCGGCGGCG GCGGCCGCGG CGGCTGCAGC TCCAGGGAGG 
    GGGTCTGAGT CGCCTGTCAC CATTTCCAGG GCTGGGAACG CCGGAGAGTT GGTCTCTCCC
    CTTCTACTGC CTCCAACACG GCGGCGGCGG CGGCGGCACA TCCAGGGACC CGGGCCGGTT
    TTAAACCTCC CGTCCGCCGC CGCCGCACCC CCCGTGGCCC GGGCTCCGGA GGCCGCCGGC
    GGAGGCAGCC GTTCGGAGGA TTATTCGTCT TCTCCCCATT CCGCTGCCGC CGCTGCCAGG
    CCTCTGGCTG CTGAGGAGAA GCAGGCCCAG TCGCTGCAAC CATCCAGCAG CCGCCGCAGC
    AGCCATTACC CGGCTGCGGT CCAGAGCCAA GCGGCGGCAG AGCGAGGGGC ATCAGCTACC
    GCCAAGTCCA GAGCCATTTC CATCCTGCAG AAGAAGCCCC GCCACCAGCA GCTTCTGCCA
    TCTCTCTCCT CCTTTTTCTT CAGCCACAGG CTCCCAGACA TGACAGCCAT CATCAAAGAG
    ATCGTTAGCA GAAACAAAAG GAGATATCAA GAGGATGGAT TCGACTTAGA CTTGACCTAT
    ATTTATCCAA ACATTATTGC TATGGGATTT CCTGCAGAAA GACTTGAAGG CGTATACAGG
    AACAATATTG ATGATGTAGT AAGGTTTTTG GATTCAAAGC ATAAAAACCA TTACAAGATA
    TACAATCTTT GTGCTGAAAG ACATTATGAC ACCGCCAAAT TTAATTGCAG AGTTGCACAA
    TATCCTTTTG AAGACCATAA CCCACCACAG CTAGAACTTA TCAAACCCTT TTGTGAAGAT
    CTTGACCAAT GGCTAAGTGA AGATGACAAT CATGTTGCAG CAATTCACTG TAAAGCTGGA
    AAGGGACGAA CTGGTGTAAT GATATGTGCA TATTTATTAC ATCGGGGCAA ATTTTTAAAG
    GCACAAGAGG CCCTAGATTT CTATGGGGAA GTAAGGACCA GAGACAAAAA GGGAGTAACT
    ATTCCCAGTC AGAGGCGCTA TGTGTATTAT TATAGCTACC TGTTAAAGAA TCATCTGGAT
    TATAGACCAG TGGCACTGTT GTTTCACAAG ATGATGTTTG AAACTATTCC AATGTTCAGT
    GGCGGAACTT GCAATCCTCA GTTTGTGGTC TGCCAGCTAA AGGTGAAGAT ATATTCCTCC
    AATTCAGGAC CCACACGACG GGAAGACAAG TTCATGTACT TTGAGTTCCC TCAGCCGTTA
    CCTGTGTGTG GTGATATCAA AGTAGAGTTC TTCCACAAAC AGAACAAGAT GCTAAAAAAG
    GACAAAATGT TTCACTTTTG GGTAAATACA TTCTTCATAC CAGGACCAGA GGAAACCTCA
    GAAAAAGTAG AAAATGGAAG TCTATGTGAT CAAGAAATCG ATAGCATTTG CAGTATAGAG
    CGTGCAGATA ATGACAAGGA ATATCTAGTA CTTACTTTAA CAAAAAATGA TCTTGACAAA
    GCAAATAAAG ACAAAGCCAA CCGATACTTT TCTCCAAATT TTAAGGTGAA GCTGTACTTC
    ACAAAAACAG TAGAGGAGCC GTCAAATCCA GAGGCTAGCA GTTCAACTTC TGTAACACCA
    GATGTTAGTG ACAATGAACC TGATCATTAT AGATATTCTG ACACCACTGA CTCTGATCCA
    GAGAATGAAC CTTTTGATGA AGATCAGCAT ACACAAATTA CAAAAGTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu59999
    Clone ID Related Accession (Same CDS sequence) NM_001304717.2 , NM_001304717.5
    Accession Version NM_001304717.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1731bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-31
    Organism Homo sapiens(human)
    Product phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN isoform PTEN-L
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U92436.1, BC005821.2, AC063965.8 and AA836562.1. On Nov 26, 2018 this sequence version replaced NM_001304717.4. Transcript Variant: This variant (1) encodes multiple isoforms due to the use of alternative translation initiation codons. The longest isoform (PTEN-L, PMID:23744781; also known as PTENalpha, PMID: 24768297) is derived from the use of an upstream non-AUG (CUG) start codon, while two shorter isoforms are derived from downstream AUG start codons. PTEN-L, initiates with a Leucine, rather than a Methionine, is thought to be preferentially associated with the mitochondrial inner membrane (PMID: 24768297), and is represented in this RefSeq. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U92436.1, SRR1660807.191185.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 23744781, 24768297 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    CTGGAGCGGG GGGGAGAAGC GGCGGCGGCG GCGGCCGCGG CGGCTGCAGC TCCAGGGAGG 
    GGGTCTGAGT CGCCTGTCAC CATTTCCAGG GCTGGGAACG CCGGAGAGTT GGTCTCTCCC
    CTTCTACTGC CTCCAACACG GCGGCGGCGG CGGCGGCACA TCCAGGGACC CGGGCCGGTT
    TTAAACCTCC CGTCCGCCGC CGCCGCACCC CCCGTGGCCC GGGCTCCGGA GGCCGCCGGC
    GGAGGCAGCC GTTCGGAGGA TTATTCGTCT TCTCCCCATT CCGCTGCCGC CGCTGCCAGG
    CCTCTGGCTG CTGAGGAGAA GCAGGCCCAG TCGCTGCAAC CATCCAGCAG CCGCCGCAGC
    AGCCATTACC CGGCTGCGGT CCAGAGCCAA GCGGCGGCAG AGCGAGGGGC ATCAGCTACC
    GCCAAGTCCA GAGCCATTTC CATCCTGCAG AAGAAGCCCC GCCACCAGCA GCTTCTGCCA
    TCTCTCTCCT CCTTTTTCTT CAGCCACAGG CTCCCAGACA TGACAGCCAT CATCAAAGAG
    ATCGTTAGCA GAAACAAAAG GAGATATCAA GAGGATGGAT TCGACTTAGA CTTGACCTAT
    ATTTATCCAA ACATTATTGC TATGGGATTT CCTGCAGAAA GACTTGAAGG CGTATACAGG
    AACAATATTG ATGATGTAGT AAGGTTTTTG GATTCAAAGC ATAAAAACCA TTACAAGATA
    TACAATCTTT GTGCTGAAAG ACATTATGAC ACCGCCAAAT TTAATTGCAG AGTTGCACAA
    TATCCTTTTG AAGACCATAA CCCACCACAG CTAGAACTTA TCAAACCCTT TTGTGAAGAT
    CTTGACCAAT GGCTAAGTGA AGATGACAAT CATGTTGCAG CAATTCACTG TAAAGCTGGA
    AAGGGACGAA CTGGTGTAAT GATATGTGCA TATTTATTAC ATCGGGGCAA ATTTTTAAAG
    GCACAAGAGG CCCTAGATTT CTATGGGGAA GTAAGGACCA GAGACAAAAA GGGAGTAACT
    ATTCCCAGTC AGAGGCGCTA TGTGTATTAT TATAGCTACC TGTTAAAGAA TCATCTGGAT
    TATAGACCAG TGGCACTGTT GTTTCACAAG ATGATGTTTG AAACTATTCC AATGTTCAGT
    GGCGGAACTT GCAATCCTCA GTTTGTGGTC TGCCAGCTAA AGGTGAAGAT ATATTCCTCC
    AATTCAGGAC CCACACGACG GGAAGACAAG TTCATGTACT TTGAGTTCCC TCAGCCGTTA
    CCTGTGTGTG GTGATATCAA AGTAGAGTTC TTCCACAAAC AGAACAAGAT GCTAAAAAAG
    GACAAAATGT TTCACTTTTG GGTAAATACA TTCTTCATAC CAGGACCAGA GGAAACCTCA
    GAAAAAGTAG AAAATGGAAG TCTATGTGAT CAAGAAATCG ATAGCATTTG CAGTATAGAG
    CGTGCAGATA ATGACAAGGA ATATCTAGTA CTTACTTTAA CAAAAAATGA TCTTGACAAA
    GCAAATAAAG ACAAAGCCAA CCGATACTTT TCTCCAAATT TTAAGGTGAA GCTGTACTTC
    ACAAAAACAG TAGAGGAGCC GTCAAATCCA GAGGCTAGCA GTTCAACTTC TGTAACACCA
    GATGTTAGTG ACAATGAACC TGATCATTAT AGATATTCTG ACACCACTGA CTCTGATCCA
    GAGAATGAAC CTTTTGATGA AGATCAGCAT ACACAAATTA CAAAAGTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001291646.4
    CDS326..2056
    Translation

    Target ORF information:

    RefSeq Version NM_001304717.5
    Organism Homo sapiens(human)
    Definition Homo sapiens phosphatase and tensin homolog (PTEN), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001304717.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    CTGGAGCGGG GGGGAGAAGC GGCGGCGGCG GCGGCCGCGG CGGCTGCAGC TCCAGGGAGG 
    GGGTCTGAGT CGCCTGTCAC CATTTCCAGG GCTGGGAACG CCGGAGAGTT GGTCTCTCCC
    CTTCTACTGC CTCCAACACG GCGGCGGCGG CGGCGGCACA TCCAGGGACC CGGGCCGGTT
    TTAAACCTCC CGTCCGCCGC CGCCGCACCC CCCGTGGCCC GGGCTCCGGA GGCCGCCGGC
    GGAGGCAGCC GTTCGGAGGA TTATTCGTCT TCTCCCCATT CCGCTGCCGC CGCTGCCAGG
    CCTCTGGCTG CTGAGGAGAA GCAGGCCCAG TCGCTGCAAC CATCCAGCAG CCGCCGCAGC
    AGCCATTACC CGGCTGCGGT CCAGAGCCAA GCGGCGGCAG AGCGAGGGGC ATCAGCTACC
    GCCAAGTCCA GAGCCATTTC CATCCTGCAG AAGAAGCCCC GCCACCAGCA GCTTCTGCCA
    TCTCTCTCCT CCTTTTTCTT CAGCCACAGG CTCCCAGACA TGACAGCCAT CATCAAAGAG
    ATCGTTAGCA GAAACAAAAG GAGATATCAA GAGGATGGAT TCGACTTAGA CTTGACCTAT
    ATTTATCCAA ACATTATTGC TATGGGATTT CCTGCAGAAA GACTTGAAGG CGTATACAGG
    AACAATATTG ATGATGTAGT AAGGTTTTTG GATTCAAAGC ATAAAAACCA TTACAAGATA
    TACAATCTTT GTGCTGAAAG ACATTATGAC ACCGCCAAAT TTAATTGCAG AGTTGCACAA
    TATCCTTTTG AAGACCATAA CCCACCACAG CTAGAACTTA TCAAACCCTT TTGTGAAGAT
    CTTGACCAAT GGCTAAGTGA AGATGACAAT CATGTTGCAG CAATTCACTG TAAAGCTGGA
    AAGGGACGAA CTGGTGTAAT GATATGTGCA TATTTATTAC ATCGGGGCAA ATTTTTAAAG
    GCACAAGAGG CCCTAGATTT CTATGGGGAA GTAAGGACCA GAGACAAAAA GGGAGTAACT
    ATTCCCAGTC AGAGGCGCTA TGTGTATTAT TATAGCTACC TGTTAAAGAA TCATCTGGAT
    TATAGACCAG TGGCACTGTT GTTTCACAAG ATGATGTTTG AAACTATTCC AATGTTCAGT
    GGCGGAACTT GCAATCCTCA GTTTGTGGTC TGCCAGCTAA AGGTGAAGAT ATATTCCTCC
    AATTCAGGAC CCACACGACG GGAAGACAAG TTCATGTACT TTGAGTTCCC TCAGCCGTTA
    CCTGTGTGTG GTGATATCAA AGTAGAGTTC TTCCACAAAC AGAACAAGAT GCTAAAAAAG
    GACAAAATGT TTCACTTTTG GGTAAATACA TTCTTCATAC CAGGACCAGA GGAAACCTCA
    GAAAAAGTAG AAAATGGAAG TCTATGTGAT CAAGAAATCG ATAGCATTTG CAGTATAGAG
    CGTGCAGATA ATGACAAGGA ATATCTAGTA CTTACTTTAA CAAAAAATGA TCTTGACAAA
    GCAAATAAAG ACAAAGCCAA CCGATACTTT TCTCCAAATT TTAAGGTGAA GCTGTACTTC
    ACAAAAACAG TAGAGGAGCC GTCAAATCCA GAGGCTAGCA GTTCAACTTC TGTAACACCA
    GATGTTAGTG ACAATGAACC TGATCATTAT AGATATTCTG ACACCACTGA CTCTGATCCA
    GAGAATGAAC CTTTTGATGA AGATCAGCAT ACACAAATTA CAAAAGTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60002
    Clone ID Related Accession (Same CDS sequence) NM_001304718.2 , NM_001304718.1
    Accession Version NM_001304718.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 621bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-22
    Organism Homo sapiens(human)
    Product phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U92436.1, BC005821.2, JF268690.1, AC063965.8 and AA836562.1. On Nov 21, 2018 this sequence version replaced NM_001304718.1. Transcript Variant: This variant (2) both contains and lacks alternate exons in its 5' UTR compared to variant 1. This variant represents translation initiation at a downstream AUG compared to the more upstream CUG and AUG start codons, as used in variant 1. Use of the more 5' initiation codons, as used in variant 1, is associated with a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the downstream AUG to encode an isoform (3) that has a shorter N-terminus, compared to isoform PTENalpha and PTEN. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: JF268690.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGATGTTTG AAACTATTCC AATGTTCAGT GGCGGAACTT GCAATCCTCA GTTTGTGGTC 
    TGCCAGCTAA AGGTGAAGAT ATATTCCTCC AATTCAGGAC CCACACGACG GGAAGACAAG
    TTCATGTACT TTGAGTTCCC TCAGCCGTTA CCTGTGTGTG GTGATATCAA AGTAGAGTTC
    TTCCACAAAC AGAACAAGAT GCTAAAAAAG GACAAAATGT TTCACTTTTG GGTAAATACA
    TTCTTCATAC CAGGACCAGA GGAAACCTCA GAAAAAGTAG AAAATGGAAG TCTATGTGAT
    CAAGAAATCG ATAGCATTTG CAGTATAGAG CGTGCAGATA ATGACAAGGA ATATCTAGTA
    CTTACTTTAA CAAAAAATGA TCTTGACAAA GCAAATAAAG ACAAAGCCAA CCGATACTTT
    TCTCCAAATT TTAAGGTGAA GCTGTACTTC ACAAAAACAG TAGAGGAGCC GTCAAATCCA
    GAGGCTAGCA GTTCAACTTC TGTAACACCA GATGTTAGTG ACAATGAACC TGATCATTAT
    AGATATTCTG ACACCACTGA CTCTGATCCA GAGAATGAAC CTTTTGATGA AGATCAGCAT
    ACACAAATTA CAAAAGTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001291647.1
    CDS1550..2170
    Translation

    Target ORF information:

    RefSeq Version NM_001304718.2
    Organism Homo sapiens(human)
    Definition Homo sapiens phosphatase and tensin homolog (PTEN), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001304718.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGATGTTTG AAACTATTCC AATGTTCAGT GGCGGAACTT GCAATCCTCA GTTTGTGGTC 
    TGCCAGCTAA AGGTGAAGAT ATATTCCTCC AATTCAGGAC CCACACGACG GGAAGACAAG
    TTCATGTACT TTGAGTTCCC TCAGCCGTTA CCTGTGTGTG GTGATATCAA AGTAGAGTTC
    TTCCACAAAC AGAACAAGAT GCTAAAAAAG GACAAAATGT TTCACTTTTG GGTAAATACA
    TTCTTCATAC CAGGACCAGA GGAAACCTCA GAAAAAGTAG AAAATGGAAG TCTATGTGAT
    CAAGAAATCG ATAGCATTTG CAGTATAGAG CGTGCAGATA ATGACAAGGA ATATCTAGTA
    CTTACTTTAA CAAAAAATGA TCTTGACAAA GCAAATAAAG ACAAAGCCAA CCGATACTTT
    TCTCCAAATT TTAAGGTGAA GCTGTACTTC ACAAAAACAG TAGAGGAGCC GTCAAATCCA
    GAGGCTAGCA GTTCAACTTC TGTAACACCA GATGTTAGTG ACAATGAACC TGATCATTAT
    AGATATTCTG ACACCACTGA CTCTGATCCA GAGAATGAAC CTTTTGATGA AGATCAGCAT
    ACACAAATTA CAAAAGTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60002
    Clone ID Related Accession (Same CDS sequence) NM_001304718.2 , NM_001304718.1
    Accession Version NM_001304718.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 621bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-31
    Organism Homo sapiens(human)
    Product phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U92436.1, AC063965.8, BC005821.2, JF268690.1 and AA836562.1. Transcript Variant: This variant (2) both contains and lacks alternate exons in its 5' UTR compared to variant 1. This variant represents translation initiation at a downstream AUG compared to the more upstream CUG and AUG start codons, as used in variant 1. Use of the more 5' initiation codons, as used in variant 1, is associated with a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the downstream AUG to encode an isoform (3) that has a shorter N-terminus, compared to isoform PTENalpha and PTEN. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: JF268690.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGATGTTTG AAACTATTCC AATGTTCAGT GGCGGAACTT GCAATCCTCA GTTTGTGGTC 
    TGCCAGCTAA AGGTGAAGAT ATATTCCTCC AATTCAGGAC CCACACGACG GGAAGACAAG
    TTCATGTACT TTGAGTTCCC TCAGCCGTTA CCTGTGTGTG GTGATATCAA AGTAGAGTTC
    TTCCACAAAC AGAACAAGAT GCTAAAAAAG GACAAAATGT TTCACTTTTG GGTAAATACA
    TTCTTCATAC CAGGACCAGA GGAAACCTCA GAAAAAGTAG AAAATGGAAG TCTATGTGAT
    CAAGAAATCG ATAGCATTTG CAGTATAGAG CGTGCAGATA ATGACAAGGA ATATCTAGTA
    CTTACTTTAA CAAAAAATGA TCTTGACAAA GCAAATAAAG ACAAAGCCAA CCGATACTTT
    TCTCCAAATT TTAAGGTGAA GCTGTACTTC ACAAAAACAG TAGAGGAGCC GTCAAATCCA
    GAGGCTAGCA GTTCAACTTC TGTAACACCA GATGTTAGTG ACAATGAACC TGATCATTAT
    AGATATTCTG ACACCACTGA CTCTGATCCA GAGAATGAAC CTTTTGATGA AGATCAGCAT
    ACACAAATTA CAAAAGTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001291647.1
    CDS1737..2357
    Translation

    Target ORF information:

    RefSeq Version NM_001304718.1
    Organism Homo sapiens(human)
    Definition Homo sapiens phosphatase and tensin homolog (PTEN), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001304718.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGATGTTTG AAACTATTCC AATGTTCAGT GGCGGAACTT GCAATCCTCA GTTTGTGGTC 
    TGCCAGCTAA AGGTGAAGAT ATATTCCTCC AATTCAGGAC CCACACGACG GGAAGACAAG
    TTCATGTACT TTGAGTTCCC TCAGCCGTTA CCTGTGTGTG GTGATATCAA AGTAGAGTTC
    TTCCACAAAC AGAACAAGAT GCTAAAAAAG GACAAAATGT TTCACTTTTG GGTAAATACA
    TTCTTCATAC CAGGACCAGA GGAAACCTCA GAAAAAGTAG AAAATGGAAG TCTATGTGAT
    CAAGAAATCG ATAGCATTTG CAGTATAGAG CGTGCAGATA ATGACAAGGA ATATCTAGTA
    CTTACTTTAA CAAAAAATGA TCTTGACAAA GCAAATAAAG ACAAAGCCAA CCGATACTTT
    TCTCCAAATT TTAAGGTGAA GCTGTACTTC ACAAAAACAG TAGAGGAGCC GTCAAATCCA
    GAGGCTAGCA GTTCAACTTC TGTAACACCA GATGTTAGTG ACAATGAACC TGATCATTAT
    AGATATTCTG ACACCACTGA CTCTGATCCA GAGAATGAAC CTTTTGATGA AGATCAGCAT
    ACACAAATTA CAAAAGTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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