HACE1 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following HACE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HACE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu17253 NM_020771.4
Latest version!
Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu101566 NM_001350560.2
Latest version!
Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $307.30
$439.00
OHu40894 NM_001321083.2
Latest version!
Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu40895 NM_001321080.2
Latest version!
Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu40896 NM_001350557.2
Latest version!
Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu40896 NM_001321084.2
Latest version!
Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu40896 NM_001350558.2
Latest version!
Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu70565 NM_001350556.2
Latest version!
Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu70566 NM_001350559.2
Latest version!
Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu40896 XM_017011118.1
Latest version!
Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu40896 XM_017011119.2
Latest version!
Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu70566 XM_017011122.1
Latest version!
Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu101567 NM_001350555.2
Latest version!
Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu96713 NM_001350554.2
Latest version!
Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu96713 XM_017011114.2
Latest version!
Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
View Old Accession Versions >>
OHu40896 XM_017011119.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu40894 NM_001321083.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu101567 NM_001350555.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu17253 NM_020771.3 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu70566 NM_001350559.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu40896 NM_001350558.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu96713 XM_017011114.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu70565 NM_001350556.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu40895 NM_001321080.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu40896 NM_001321084.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu96713 NM_001350554.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu101566 NM_001350560.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $307.30
$439.00
OHu40896 NM_001350557.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu17253
    Clone ID Related Accession (Same CDS sequence) NM_020771.3 , NM_020771.4
    Accession Version NM_020771.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2730bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-28
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HACE1 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK131207.1, BC034982.2, BC038119.1 and AJ420474.1. This sequence is a reference standard in the RefSeqGene project. On Oct 31, 2006 this sequence version replaced NM_020771.2. Transcript Variant: This variant (1) encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC034982.2, SRR1660807.129811.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGAGAG CGATGGAGCA ACTCAACCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGTTGC CCGAGGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTCGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC GGTGGAATGC
    TTGGTTTTGC TGTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGCTGTACA
    CCCCTTCATT TGGCAGCAAG AAATGGGCAG AAGAAATGTA TGAGTAAATT ATTAGAATAT
    AGCGCTGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAATACATTG GCTGGCTGTG
    AATGGGCGGA CAGAACTACT CCATGACCTT GTGCAGCATG TCAGTGATGT TGATGTTGAG
    GATGCCATGG GGCAGACAGC ACTGCATGTT GCCTGCCAGA ACGGTCACAA GACGACAGTG
    CAGTGCTTGC TAGACAGTGG TGCTGATATT AACAGGCCAA ATGTATCAGG AGCAACTCCA
    TTGTACTTTG CTTGCAGTCA TGGTCAGAGA GATACAGCAC AGATCCTACT ATTACGAGGA
    GCCAAATATC TGCCAGATAA AAATGGAGTA ACTCCTCTGG ATTTATGTGT ACAGGGTGGA
    TATGGAGAGA CTTGTGAAGT ATTAATTCAA TATCACCCGA GGCTTTTTCA GACTATTATT
    CAAATGACAC AGAATGAAGA CCTCCGAGAA AACATGTTAC GGCAAGTTCT GGAGCATTTG
    TCTCAGCAAA GTGAAAGCCA GTACCTAAAG ATTCTAACAA GCCTTGCTGA AGTTGCTACA
    ACAAATGGTC ATAAACTGCT TAGCCTCTCT AGCAATTATG ATGCTCAAAT GAAGAGCCTT
    TTAAGGATTG TGAGAATGTT TTGTCACGTC TTTCGAATTG GTCCATCCTC CCCCAGTAAT
    GGAATTGATA TGGGCTACAA TGGGAATAAA ACTCCAAGAA GCCAGGTGTT CAAGCCTCTG
    GAATTGCTTT GGCACTCGTT AGATGAATGG CTAGTTTTAA TAGCCACAGA ATTGATGAAA
    AACAAAAGAG ACTCAACAGA GATCACTTCT ATTTTACTGA AACAAAAAGG CCAAGATCAA
    GATGCTGCTT CCATTCCTCC ATTTGAACCT CCAGGACCTG GGAGCTATGA AAATCTGTCC
    ACTGGCACAA GGGAATCTAA ACCAGATGCT CTTGCAGGGA GACAGGAAGC CAGTGCAGAT
    TGTCAGGATG TTATTTCTAT GACAGCTAAC CGGCTAAGTG CTGTCATTCA AGCTTTTTAC
    ATGTGCTGTT CTTGTCAGAT GCCTCCGGGA ATGACTTCAC CTCGTTTCAT TGAATTTGTC
    TGCAAACATG ATGAAGTTTT AAAATGCTTT GTTAATAGAA ATCCCAAAAT TATATTTGAC
    CACTTTCACT TTCTCCTTGA ATGTCCTGAG TTGATGTCAA GATTCATGCA TATCATAAAA
    GCACAGCCTT TTAAAGATCG CTGTGAATGG TTCTATGAAC ATTTGCATTC AGGACAGCCA
    GATTCAGATA TGGTGCACAG GCCAGTGAAT GAAAATGATA TCCTGCTGGT TCACAGAGAT
    TCTATTTTTA GGAGTAGCTG TGAAGTTGTG TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA
    GGGATTGCTG TACGGTTCCA TGGAGAAGAA GGCATGGGTC AAGGTGTTGT GCGTGAGTGG
    TTTGATATTC TGTCCAATGA GATAGTCAAT CCTGATTATG CATTGTTTAC CCAGTCAGCT
    GATGGAACAA CTTTTCAGCC TAATAGCAAC TCTTATGTAA ATCCTGATCA CTTGAACTAT
    TTTCGGTTTG CTGGGCAGAT CTTGGGATTA GCGTTGAACC ACAGGCAGCT GGTCAATATT
    TACTTCACAC GATCCTTCTA CAAGCACATT CTTGGTATTC CTGTAAATTA CCAAGATGTG
    GCATCCATTG ATCCAGAATA TGCGAAAAAT TTGCAATGGA TTTTAGATAA TGATATAAGT
    GATCTGGGTC TAGAACTAAC TTTTTCTGTT GAGACTGATG TGTTTGGAGC AATGGAAGAG
    GTGCCTTTGA AACCTGGGGG TGGGAGTATT CTTGTGACAC AAAATAATAA AGCGGAGTAC
    GTCCAGCTTG TTACTGAACT TCGAATGACA AGAGCCATTC AGCCTCAGAT CAATGCTTTT
    TTACAGGGCT TTCATATGTT CATTCCACCC TCCCTCATAC AGCTTTTTGA TGAATATGAA
    TTGGAGCTAC TGCTTTCTGG CATGCCAGAA ATTGATGTGA GTGATTGGAT AAAAAATACA
    GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTA
    GAAGACATTA CTCAAGAGGA GAGAGTTCTT CTCTTACAGT TTGTTACGGG CAGTTCCAGG
    GTCCCACATG GTGGGTTTGC TAATATCATG GGTGGAAGTG GATTGCAAAA CTTTACAATC
    GCTGCTGTGC CATATACTCC AAATCTTTTA CCAACTTCAA GCACATGCAT CAACATGCTC
    AAGTTACCTG AATACCCAAG TAAAGAAATA CTCAAGGACA GACTTCTTGT GGCACTACAT
    TGTGGCAGCT ATGGTTACAC AATGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_065822.2
    CDS278..3007
    Translation

    Target ORF information:

    RefSeq Version NM_020771.3
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_020771.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGAGAG CGATGGAGCA ACTCAACCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGTTGC CCGAGGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTCGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC GGTGGAATGC
    TTGGTTTTGC TGTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGCTGTACA
    CCCCTTCATT TGGCAGCAAG AAATGGGCAG AAGAAATGTA TGAGTAAATT ATTAGAATAT
    AGCGCTGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAATACATTG GCTGGCTGTG
    AATGGGCGGA CAGAACTACT CCATGACCTT GTGCAGCATG TCAGTGATGT TGATGTTGAG
    GATGCCATGG GGCAGACAGC ACTGCATGTT GCCTGCCAGA ACGGTCACAA GACGACAGTG
    CAGTGCTTGC TAGACAGTGG TGCTGATATT AACAGGCCAA ATGTATCAGG AGCAACTCCA
    TTGTACTTTG CTTGCAGTCA TGGTCAGAGA GATACAGCAC AGATCCTACT ATTACGAGGA
    GCCAAATATC TGCCAGATAA AAATGGAGTA ACTCCTCTGG ATTTATGTGT ACAGGGTGGA
    TATGGAGAGA CTTGTGAAGT ATTAATTCAA TATCACCCGA GGCTTTTTCA GACTATTATT
    CAAATGACAC AGAATGAAGA CCTCCGAGAA AACATGTTAC GGCAAGTTCT GGAGCATTTG
    TCTCAGCAAA GTGAAAGCCA GTACCTAAAG ATTCTAACAA GCCTTGCTGA AGTTGCTACA
    ACAAATGGTC ATAAACTGCT TAGCCTCTCT AGCAATTATG ATGCTCAAAT GAAGAGCCTT
    TTAAGGATTG TGAGAATGTT TTGTCACGTC TTTCGAATTG GTCCATCCTC CCCCAGTAAT
    GGAATTGATA TGGGCTACAA TGGGAATAAA ACTCCAAGAA GCCAGGTGTT CAAGCCTCTG
    GAATTGCTTT GGCACTCGTT AGATGAATGG CTAGTTTTAA TAGCCACAGA ATTGATGAAA
    AACAAAAGAG ACTCAACAGA GATCACTTCT ATTTTACTGA AACAAAAAGG CCAAGATCAA
    GATGCTGCTT CCATTCCTCC ATTTGAACCT CCAGGACCTG GGAGCTATGA AAATCTGTCC
    ACTGGCACAA GGGAATCTAA ACCAGATGCT CTTGCAGGGA GACAGGAAGC CAGTGCAGAT
    TGTCAGGATG TTATTTCTAT GACAGCTAAC CGGCTAAGTG CTGTCATTCA AGCTTTTTAC
    ATGTGCTGTT CTTGTCAGAT GCCTCCGGGA ATGACTTCAC CTCGTTTCAT TGAATTTGTC
    TGCAAACATG ATGAAGTTTT AAAATGCTTT GTTAATAGAA ATCCCAAAAT TATATTTGAC
    CACTTTCACT TTCTCCTTGA ATGTCCTGAG TTGATGTCAA GATTCATGCA TATCATAAAA
    GCACAGCCTT TTAAAGATCG CTGTGAATGG TTCTATGAAC ATTTGCATTC AGGACAGCCA
    GATTCAGATA TGGTGCACAG GCCAGTGAAT GAAAATGATA TCCTGCTGGT TCACAGAGAT
    TCTATTTTTA GGAGTAGCTG TGAAGTTGTG TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA
    GGGATTGCTG TACGGTTCCA TGGAGAAGAA GGCATGGGTC AAGGTGTTGT GCGTGAGTGG
    TTTGATATTC TGTCCAATGA GATAGTCAAT CCTGATTATG CATTGTTTAC CCAGTCAGCT
    GATGGAACAA CTTTTCAGCC TAATAGCAAC TCTTATGTAA ATCCTGATCA CTTGAACTAT
    TTTCGGTTTG CTGGGCAGAT CTTGGGATTA GCGTTGAACC ACAGGCAGCT GGTCAATATT
    TACTTCACAC GATCCTTCTA CAAGCACATT CTTGGTATTC CTGTAAATTA CCAAGATGTG
    GCATCCATTG ATCCAGAATA TGCGAAAAAT TTGCAATGGA TTTTAGATAA TGATATAAGT
    GATCTGGGTC TAGAACTAAC TTTTTCTGTT GAGACTGATG TGTTTGGAGC AATGGAAGAG
    GTGCCTTTGA AACCTGGGGG TGGGAGTATT CTTGTGACAC AAAATAATAA AGCGGAGTAC
    GTCCAGCTTG TTACTGAACT TCGAATGACA AGAGCCATTC AGCCTCAGAT CAATGCTTTT
    TTACAGGGCT TTCATATGTT CATTCCACCC TCCCTCATAC AGCTTTTTGA TGAATATGAA
    TTGGAGCTAC TGCTTTCTGG CATGCCAGAA ATTGATGTGA GTGATTGGAT AAAAAATACA
    GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTA
    GAAGACATTA CTCAAGAGGA GAGAGTTCTT CTCTTACAGT TTGTTACGGG CAGTTCCAGG
    GTCCCACATG GTGGGTTTGC TAATATCATG GGTGGAAGTG GATTGCAAAA CTTTACAATC
    GCTGCTGTGC CATATACTCC AAATCTTTTA CCAACTTCAA GCACATGCAT CAACATGCTC
    AAGTTACCTG AATACCCAAG TAAAGAAATA CTCAAGGACA GACTTCTTGT GGCACTACAT
    TGTGGCAGCT ATGGTTACAC AATGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17253
    Clone ID Related Accession (Same CDS sequence) NM_020771.3 , NM_020771.4
    Accession Version NM_020771.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2730bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-04
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HACE1 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK131207.1, BC034982.2, BC038119.1 and AJ420474.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_020771.3. Transcript Variant: This variant (1) encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.248387.1, SRR1660807.129811.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000262903.9/ ENSP00000262903.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGAGAG CGATGGAGCA ACTCAACCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGTTGC CCGAGGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTCGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC GGTGGAATGC
    TTGGTTTTGC TGTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGCTGTACA
    CCCCTTCATT TGGCAGCAAG AAATGGGCAG AAGAAATGTA TGAGTAAATT ATTAGAATAT
    AGCGCTGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAATACATTG GCTGGCTGTG
    AATGGGCGGA CAGAACTACT CCATGACCTT GTGCAGCATG TCAGTGATGT TGATGTTGAG
    GATGCCATGG GGCAGACAGC ACTGCATGTT GCCTGCCAGA ACGGTCACAA GACGACAGTG
    CAGTGCTTGC TAGACAGTGG TGCTGATATT AACAGGCCAA ATGTATCAGG AGCAACTCCA
    TTGTACTTTG CTTGCAGTCA TGGTCAGAGA GATACAGCAC AGATCCTACT ATTACGAGGA
    GCCAAATATC TGCCAGATAA AAATGGAGTA ACTCCTCTGG ATTTATGTGT ACAGGGTGGA
    TATGGAGAGA CTTGTGAAGT ATTAATTCAA TATCACCCGA GGCTTTTTCA GACTATTATT
    CAAATGACAC AGAATGAAGA CCTCCGAGAA AACATGTTAC GGCAAGTTCT GGAGCATTTG
    TCTCAGCAAA GTGAAAGCCA GTACCTAAAG ATTCTAACAA GCCTTGCTGA AGTTGCTACA
    ACAAATGGTC ATAAACTGCT TAGCCTCTCT AGCAATTATG ATGCTCAAAT GAAGAGCCTT
    TTAAGGATTG TGAGAATGTT TTGTCACGTC TTTCGAATTG GTCCATCCTC CCCCAGTAAT
    GGAATTGATA TGGGCTACAA TGGGAATAAA ACTCCAAGAA GCCAGGTGTT CAAGCCTCTG
    GAATTGCTTT GGCACTCGTT AGATGAATGG CTAGTTTTAA TAGCCACAGA ATTGATGAAA
    AACAAAAGAG ACTCAACAGA GATCACTTCT ATTTTACTGA AACAAAAAGG CCAAGATCAA
    GATGCTGCTT CCATTCCTCC ATTTGAACCT CCAGGACCTG GGAGCTATGA AAATCTGTCC
    ACTGGCACAA GGGAATCTAA ACCAGATGCT CTTGCAGGGA GACAGGAAGC CAGTGCAGAT
    TGTCAGGATG TTATTTCTAT GACAGCTAAC CGGCTAAGTG CTGTCATTCA AGCTTTTTAC
    ATGTGCTGTT CTTGTCAGAT GCCTCCGGGA ATGACTTCAC CTCGTTTCAT TGAATTTGTC
    TGCAAACATG ATGAAGTTTT AAAATGCTTT GTTAATAGAA ATCCCAAAAT TATATTTGAC
    CACTTTCACT TTCTCCTTGA ATGTCCTGAG TTGATGTCAA GATTCATGCA TATCATAAAA
    GCACAGCCTT TTAAAGATCG CTGTGAATGG TTCTATGAAC ATTTGCATTC AGGACAGCCA
    GATTCAGATA TGGTGCACAG GCCAGTGAAT GAAAATGATA TCCTGCTGGT TCACAGAGAT
    TCTATTTTTA GGAGTAGCTG TGAAGTTGTG TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA
    GGGATTGCTG TACGGTTCCA TGGAGAAGAA GGCATGGGTC AAGGTGTTGT GCGTGAGTGG
    TTTGATATTC TGTCCAATGA GATAGTCAAT CCTGATTATG CATTGTTTAC CCAGTCAGCT
    GATGGAACAA CTTTTCAGCC TAATAGCAAC TCTTATGTAA ATCCTGATCA CTTGAACTAT
    TTTCGGTTTG CTGGGCAGAT CTTGGGATTA GCGTTGAACC ACAGGCAGCT GGTCAATATT
    TACTTCACAC GATCCTTCTA CAAGCACATT CTTGGTATTC CTGTAAATTA CCAAGATGTG
    GCATCCATTG ATCCAGAATA TGCGAAAAAT TTGCAATGGA TTTTAGATAA TGATATAAGT
    GATCTGGGTC TAGAACTAAC TTTTTCTGTT GAGACTGATG TGTTTGGAGC AATGGAAGAG
    GTGCCTTTGA AACCTGGGGG TGGGAGTATT CTTGTGACAC AAAATAATAA AGCGGAGTAC
    GTCCAGCTTG TTACTGAACT TCGAATGACA AGAGCCATTC AGCCTCAGAT CAATGCTTTT
    TTACAGGGCT TTCATATGTT CATTCCACCC TCCCTCATAC AGCTTTTTGA TGAATATGAA
    TTGGAGCTAC TGCTTTCTGG CATGCCAGAA ATTGATGTGA GTGATTGGAT AAAAAATACA
    GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTA
    GAAGACATTA CTCAAGAGGA GAGAGTTCTT CTCTTACAGT TTGTTACGGG CAGTTCCAGG
    GTCCCACATG GTGGGTTTGC TAATATCATG GGTGGAAGTG GATTGCAAAA CTTTACAATC
    GCTGCTGTGC CATATACTCC AAATCTTTTA CCAACTTCAA GCACATGCAT CAACATGCTC
    AAGTTACCTG AATACCCAAG TAAAGAAATA CTCAAGGACA GACTTCTTGT GGCACTACAT
    TGTGGCAGCT ATGGTTACAC AATGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_065822.2
    CDS278..3007
    Translation

    Target ORF information:

    RefSeq Version NM_020771.4
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_020771.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGAGAG CGATGGAGCA ACTCAACCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGTTGC CCGAGGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTCGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC GGTGGAATGC
    TTGGTTTTGC TGTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGCTGTACA
    CCCCTTCATT TGGCAGCAAG AAATGGGCAG AAGAAATGTA TGAGTAAATT ATTAGAATAT
    AGCGCTGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAATACATTG GCTGGCTGTG
    AATGGGCGGA CAGAACTACT CCATGACCTT GTGCAGCATG TCAGTGATGT TGATGTTGAG
    GATGCCATGG GGCAGACAGC ACTGCATGTT GCCTGCCAGA ACGGTCACAA GACGACAGTG
    CAGTGCTTGC TAGACAGTGG TGCTGATATT AACAGGCCAA ATGTATCAGG AGCAACTCCA
    TTGTACTTTG CTTGCAGTCA TGGTCAGAGA GATACAGCAC AGATCCTACT ATTACGAGGA
    GCCAAATATC TGCCAGATAA AAATGGAGTA ACTCCTCTGG ATTTATGTGT ACAGGGTGGA
    TATGGAGAGA CTTGTGAAGT ATTAATTCAA TATCACCCGA GGCTTTTTCA GACTATTATT
    CAAATGACAC AGAATGAAGA CCTCCGAGAA AACATGTTAC GGCAAGTTCT GGAGCATTTG
    TCTCAGCAAA GTGAAAGCCA GTACCTAAAG ATTCTAACAA GCCTTGCTGA AGTTGCTACA
    ACAAATGGTC ATAAACTGCT TAGCCTCTCT AGCAATTATG ATGCTCAAAT GAAGAGCCTT
    TTAAGGATTG TGAGAATGTT TTGTCACGTC TTTCGAATTG GTCCATCCTC CCCCAGTAAT
    GGAATTGATA TGGGCTACAA TGGGAATAAA ACTCCAAGAA GCCAGGTGTT CAAGCCTCTG
    GAATTGCTTT GGCACTCGTT AGATGAATGG CTAGTTTTAA TAGCCACAGA ATTGATGAAA
    AACAAAAGAG ACTCAACAGA GATCACTTCT ATTTTACTGA AACAAAAAGG CCAAGATCAA
    GATGCTGCTT CCATTCCTCC ATTTGAACCT CCAGGACCTG GGAGCTATGA AAATCTGTCC
    ACTGGCACAA GGGAATCTAA ACCAGATGCT CTTGCAGGGA GACAGGAAGC CAGTGCAGAT
    TGTCAGGATG TTATTTCTAT GACAGCTAAC CGGCTAAGTG CTGTCATTCA AGCTTTTTAC
    ATGTGCTGTT CTTGTCAGAT GCCTCCGGGA ATGACTTCAC CTCGTTTCAT TGAATTTGTC
    TGCAAACATG ATGAAGTTTT AAAATGCTTT GTTAATAGAA ATCCCAAAAT TATATTTGAC
    CACTTTCACT TTCTCCTTGA ATGTCCTGAG TTGATGTCAA GATTCATGCA TATCATAAAA
    GCACAGCCTT TTAAAGATCG CTGTGAATGG TTCTATGAAC ATTTGCATTC AGGACAGCCA
    GATTCAGATA TGGTGCACAG GCCAGTGAAT GAAAATGATA TCCTGCTGGT TCACAGAGAT
    TCTATTTTTA GGAGTAGCTG TGAAGTTGTG TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA
    GGGATTGCTG TACGGTTCCA TGGAGAAGAA GGCATGGGTC AAGGTGTTGT GCGTGAGTGG
    TTTGATATTC TGTCCAATGA GATAGTCAAT CCTGATTATG CATTGTTTAC CCAGTCAGCT
    GATGGAACAA CTTTTCAGCC TAATAGCAAC TCTTATGTAA ATCCTGATCA CTTGAACTAT
    TTTCGGTTTG CTGGGCAGAT CTTGGGATTA GCGTTGAACC ACAGGCAGCT GGTCAATATT
    TACTTCACAC GATCCTTCTA CAAGCACATT CTTGGTATTC CTGTAAATTA CCAAGATGTG
    GCATCCATTG ATCCAGAATA TGCGAAAAAT TTGCAATGGA TTTTAGATAA TGATATAAGT
    GATCTGGGTC TAGAACTAAC TTTTTCTGTT GAGACTGATG TGTTTGGAGC AATGGAAGAG
    GTGCCTTTGA AACCTGGGGG TGGGAGTATT CTTGTGACAC AAAATAATAA AGCGGAGTAC
    GTCCAGCTTG TTACTGAACT TCGAATGACA AGAGCCATTC AGCCTCAGAT CAATGCTTTT
    TTACAGGGCT TTCATATGTT CATTCCACCC TCCCTCATAC AGCTTTTTGA TGAATATGAA
    TTGGAGCTAC TGCTTTCTGG CATGCCAGAA ATTGATGTGA GTGATTGGAT AAAAAATACA
    GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTA
    GAAGACATTA CTCAAGAGGA GAGAGTTCTT CTCTTACAGT TTGTTACGGG CAGTTCCAGG
    GTCCCACATG GTGGGTTTGC TAATATCATG GGTGGAAGTG GATTGCAAAA CTTTACAATC
    GCTGCTGTGC CATATACTCC AAATCTTTTA CCAACTTCAA GCACATGCAT CAACATGCTC
    AAGTTACCTG AATACCCAAG TAAAGAAATA CTCAAGGACA GACTTCTTGT GGCACTACAT
    TGTGGCAGCT ATGGTTACAC AATGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu101566
    Clone ID Related Accession (Same CDS sequence) NM_001350560.2 , NM_001350560.1
    Accession Version NM_001350560.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-04
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HACE1 isoform i
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357315.14, AL513472.9 and AL590402.10. On Jun 1, 2019 this sequence version replaced NM_001350560.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.273495.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGACACAGA ATGAAGACCT CCGAGAAAAC ATGTTACGGC AAGTTCTGGA GCATTTGTCT 
    CAGCAAAGTG AAAGCCAGTA CCTAAAGATT CTAACAAGCC TTGCTGAAGT TGCTACAACA
    AATGGTCATA AACTGCTTAG CCTCTCTAGC AATTATGATG CTCAAATGAA GAGCCTTTTA
    AGGATTGTGA GAATGTTTTG TCACGTCTTT CGAATTGGTC CATCCTCCCC CAGTAATGGA
    ATTGATATGG GCTACAATGG GAATAAAACT CCAAGAAGCC AGGTGTTCAA GCCTCTGGAA
    TTGCTTTGGC ACTCGTTAGA TGAATGGCTA GTTTTAATAG CCACAGAATT GATGAAAAAC
    AAAAGAGACT CAACAGAGAT CACTTCTATT TTACTGAAAC AAAAAGGCCA AGATCAAGAT
    GCTGCTTCCA TTCCTCCATT TGAACCTCCA GGACCTGGGA GCTATGAAAA TCTGTCCACT
    GGCACAAGGG AATCTAAACC AGATGCTCTT GCAGGGAGAC AGGAAGCCAG TGCAGATTGT
    CAGGATGTTA TTTCTATGAC AGCTAACCGG CTAAGTGCTG TCATTCAAGC TTTTTACATG
    TGCTGTTCTT GTCAGATGCC TCCGGGAATG ACTTCACCTC GTTTCATTGA ATTTGTCTGC
    AAACATGATG AAGTTTTAAA ATGCTTTGTT AATAGAAATC CCAAAATTAT ATTTGACCAC
    TTTCACTTTC TCCTTGAATG TCCTGAGTTG ATGTCAAGAT TCATGCATAT CATAAAAGCA
    CAGCCTTTTA AAGATCGCTG TGAATGGTTC TATGAACATT TGCATTCAGG ACAGCCAGAT
    TCAGATATGG TGCACAGGCC AGTGAATGAA AATGATATCC TGCTGGTTCA CAGAGATTCT
    ATTTTTAGGA GTAGCTGTGA AGTTGTGTCA AAAGCAAATT GTGCAAAGCT AAAGCAAGGG
    ATTGCTGTAC GGTTCCATGG AGAAGAAGGC ATGGGTCAAG GTGTTGTGCG TGAGTGGTTT
    GATATTCTGT CCAATGAGAT AGTCAATCCT GATTATGCAT TGTTTACCCA GTCAGCTGAT
    GGAACAACTT TTCAGCCTAA TAGCAACTCT TATGTAAATC CTGATCACTT GAACTATTTT
    CGGTTTGCTG GGCAGATCTT GGGATTAGCG TTGAACCACA GGCAGCTGGT CAATATTTAC
    TTCACACGAT CCTTCTACAA GCACATTCTT GGTATTCCTG TAAATTACCA AGATGTGGCA
    TCCATTGATC CAGAATATGC GAAAAATTTG CAATGGATTT TAGATAATGA TATAAGTGAT
    CTGGGTCTAG AACTAACTTT TTCTGTTGAG ACTGATGTGT TTGGAGCAAT GGAAGAGGTG
    CCTTTGAAAC CTGGGGGTGG GAGTATTCTT GTGACACAAA ATAATAAAGC GGAGTACGTC
    CAGCTTGTTA CTGAACTTCG AATGACAAGA GCCATTCAGC CTCAGATCAA TGCTTTTTTA
    CAGGGCTTTC ATATGTTCAT TCCACCCTCC CTCATACAGC TTTTTGATGA ATATGAATTG
    GAGCTACTGC TTTCTGGCAT GCCAGAAATT GATGTGAGTG ATTGGATAAA AAATACAGAA
    TACACAAGTG GCTATGAAAG AGAAGATCCA GTTATTCAGT GGTTCTGGGA AGTTGTAGAA
    GACATTACTC AAGAGGAGAG AGTTCTTCTC TTACAGTTTG TTACGGGCAG TTCCAGGGTC
    CCACATGGTG GGTTTGCTAA TATCATGGGT GGAAGTGGAT TGCAAAACTT TACAATCGCT
    GCTGTGCCAT ATACTCCAAA TCTTTTACCA ACTTCAAGCA CATGCATCAA CATGCTCAAG
    TTACCTGAAT ACCCAAGTAA AGAAATACTC AAGGACAGAC TTCTTGTGGC ACTACATTGT
    GGCAGCTATG GTTACACAAT GGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337489.1
    CDS853..2799
    Translation

    Target ORF information:

    RefSeq Version NM_001350560.2
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001350560.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGACACAGA ATGAAGACCT CCGAGAAAAC ATGTTACGGC AAGTTCTGGA GCATTTGTCT 
    CAGCAAAGTG AAAGCCAGTA CCTAAAGATT CTAACAAGCC TTGCTGAAGT TGCTACAACA
    AATGGTCATA AACTGCTTAG CCTCTCTAGC AATTATGATG CTCAAATGAA GAGCCTTTTA
    AGGATTGTGA GAATGTTTTG TCACGTCTTT CGAATTGGTC CATCCTCCCC CAGTAATGGA
    ATTGATATGG GCTACAATGG GAATAAAACT CCAAGAAGCC AGGTGTTCAA GCCTCTGGAA
    TTGCTTTGGC ACTCGTTAGA TGAATGGCTA GTTTTAATAG CCACAGAATT GATGAAAAAC
    AAAAGAGACT CAACAGAGAT CACTTCTATT TTACTGAAAC AAAAAGGCCA AGATCAAGAT
    GCTGCTTCCA TTCCTCCATT TGAACCTCCA GGACCTGGGA GCTATGAAAA TCTGTCCACT
    GGCACAAGGG AATCTAAACC AGATGCTCTT GCAGGGAGAC AGGAAGCCAG TGCAGATTGT
    CAGGATGTTA TTTCTATGAC AGCTAACCGG CTAAGTGCTG TCATTCAAGC TTTTTACATG
    TGCTGTTCTT GTCAGATGCC TCCGGGAATG ACTTCACCTC GTTTCATTGA ATTTGTCTGC
    AAACATGATG AAGTTTTAAA ATGCTTTGTT AATAGAAATC CCAAAATTAT ATTTGACCAC
    TTTCACTTTC TCCTTGAATG TCCTGAGTTG ATGTCAAGAT TCATGCATAT CATAAAAGCA
    CAGCCTTTTA AAGATCGCTG TGAATGGTTC TATGAACATT TGCATTCAGG ACAGCCAGAT
    TCAGATATGG TGCACAGGCC AGTGAATGAA AATGATATCC TGCTGGTTCA CAGAGATTCT
    ATTTTTAGGA GTAGCTGTGA AGTTGTGTCA AAAGCAAATT GTGCAAAGCT AAAGCAAGGG
    ATTGCTGTAC GGTTCCATGG AGAAGAAGGC ATGGGTCAAG GTGTTGTGCG TGAGTGGTTT
    GATATTCTGT CCAATGAGAT AGTCAATCCT GATTATGCAT TGTTTACCCA GTCAGCTGAT
    GGAACAACTT TTCAGCCTAA TAGCAACTCT TATGTAAATC CTGATCACTT GAACTATTTT
    CGGTTTGCTG GGCAGATCTT GGGATTAGCG TTGAACCACA GGCAGCTGGT CAATATTTAC
    TTCACACGAT CCTTCTACAA GCACATTCTT GGTATTCCTG TAAATTACCA AGATGTGGCA
    TCCATTGATC CAGAATATGC GAAAAATTTG CAATGGATTT TAGATAATGA TATAAGTGAT
    CTGGGTCTAG AACTAACTTT TTCTGTTGAG ACTGATGTGT TTGGAGCAAT GGAAGAGGTG
    CCTTTGAAAC CTGGGGGTGG GAGTATTCTT GTGACACAAA ATAATAAAGC GGAGTACGTC
    CAGCTTGTTA CTGAACTTCG AATGACAAGA GCCATTCAGC CTCAGATCAA TGCTTTTTTA
    CAGGGCTTTC ATATGTTCAT TCCACCCTCC CTCATACAGC TTTTTGATGA ATATGAATTG
    GAGCTACTGC TTTCTGGCAT GCCAGAAATT GATGTGAGTG ATTGGATAAA AAATACAGAA
    TACACAAGTG GCTATGAAAG AGAAGATCCA GTTATTCAGT GGTTCTGGGA AGTTGTAGAA
    GACATTACTC AAGAGGAGAG AGTTCTTCTC TTACAGTTTG TTACGGGCAG TTCCAGGGTC
    CCACATGGTG GGTTTGCTAA TATCATGGGT GGAAGTGGAT TGCAAAACTT TACAATCGCT
    GCTGTGCCAT ATACTCCAAA TCTTTTACCA ACTTCAAGCA CATGCATCAA CATGCTCAAG
    TTACCTGAAT ACCCAAGTAA AGAAATACTC AAGGACAGAC TTCTTGTGGC ACTACATTGT
    GGCAGCTATG GTTACACAAT GGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu101566
    Clone ID Related Accession (Same CDS sequence) NM_001350560.2 , NM_001350560.1
    Accession Version NM_001350560.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-22
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HACE1 isoform i
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357315.14, AL513472.9 and AL590402.10. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.273495.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGACACAGA ATGAAGACCT CCGAGAAAAC ATGTTACGGC AAGTTCTGGA GCATTTGTCT 
    CAGCAAAGTG AAAGCCAGTA CCTAAAGATT CTAACAAGCC TTGCTGAAGT TGCTACAACA
    AATGGTCATA AACTGCTTAG CCTCTCTAGC AATTATGATG CTCAAATGAA GAGCCTTTTA
    AGGATTGTGA GAATGTTTTG TCACGTCTTT CGAATTGGTC CATCCTCCCC CAGTAATGGA
    ATTGATATGG GCTACAATGG GAATAAAACT CCAAGAAGCC AGGTGTTCAA GCCTCTGGAA
    TTGCTTTGGC ACTCGTTAGA TGAATGGCTA GTTTTAATAG CCACAGAATT GATGAAAAAC
    AAAAGAGACT CAACAGAGAT CACTTCTATT TTACTGAAAC AAAAAGGCCA AGATCAAGAT
    GCTGCTTCCA TTCCTCCATT TGAACCTCCA GGACCTGGGA GCTATGAAAA TCTGTCCACT
    GGCACAAGGG AATCTAAACC AGATGCTCTT GCAGGGAGAC AGGAAGCCAG TGCAGATTGT
    CAGGATGTTA TTTCTATGAC AGCTAACCGG CTAAGTGCTG TCATTCAAGC TTTTTACATG
    TGCTGTTCTT GTCAGATGCC TCCGGGAATG ACTTCACCTC GTTTCATTGA ATTTGTCTGC
    AAACATGATG AAGTTTTAAA ATGCTTTGTT AATAGAAATC CCAAAATTAT ATTTGACCAC
    TTTCACTTTC TCCTTGAATG TCCTGAGTTG ATGTCAAGAT TCATGCATAT CATAAAAGCA
    CAGCCTTTTA AAGATCGCTG TGAATGGTTC TATGAACATT TGCATTCAGG ACAGCCAGAT
    TCAGATATGG TGCACAGGCC AGTGAATGAA AATGATATCC TGCTGGTTCA CAGAGATTCT
    ATTTTTAGGA GTAGCTGTGA AGTTGTGTCA AAAGCAAATT GTGCAAAGCT AAAGCAAGGG
    ATTGCTGTAC GGTTCCATGG AGAAGAAGGC ATGGGTCAAG GTGTTGTGCG TGAGTGGTTT
    GATATTCTGT CCAATGAGAT AGTCAATCCT GATTATGCAT TGTTTACCCA GTCAGCTGAT
    GGAACAACTT TTCAGCCTAA TAGCAACTCT TATGTAAATC CTGATCACTT GAACTATTTT
    CGGTTTGCTG GGCAGATCTT GGGATTAGCG TTGAACCACA GGCAGCTGGT CAATATTTAC
    TTCACACGAT CCTTCTACAA GCACATTCTT GGTATTCCTG TAAATTACCA AGATGTGGCA
    TCCATTGATC CAGAATATGC GAAAAATTTG CAATGGATTT TAGATAATGA TATAAGTGAT
    CTGGGTCTAG AACTAACTTT TTCTGTTGAG ACTGATGTGT TTGGAGCAAT GGAAGAGGTG
    CCTTTGAAAC CTGGGGGTGG GAGTATTCTT GTGACACAAA ATAATAAAGC GGAGTACGTC
    CAGCTTGTTA CTGAACTTCG AATGACAAGA GCCATTCAGC CTCAGATCAA TGCTTTTTTA
    CAGGGCTTTC ATATGTTCAT TCCACCCTCC CTCATACAGC TTTTTGATGA ATATGAATTG
    GAGCTACTGC TTTCTGGCAT GCCAGAAATT GATGTGAGTG ATTGGATAAA AAATACAGAA
    TACACAAGTG GCTATGAAAG AGAAGATCCA GTTATTCAGT GGTTCTGGGA AGTTGTAGAA
    GACATTACTC AAGAGGAGAG AGTTCTTCTC TTACAGTTTG TTACGGGCAG TTCCAGGGTC
    CCACATGGTG GGTTTGCTAA TATCATGGGT GGAAGTGGAT TGCAAAACTT TACAATCGCT
    GCTGTGCCAT ATACTCCAAA TCTTTTACCA ACTTCAAGCA CATGCATCAA CATGCTCAAG
    TTACCTGAAT ACCCAAGTAA AGAAATACTC AAGGACAGAC TTCTTGTGGC ACTACATTGT
    GGCAGCTATG GTTACACAAT GGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337489.1
    CDS853..2799
    Translation

    Target ORF information:

    RefSeq Version NM_001350560.1
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001350560.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGACACAGA ATGAAGACCT CCGAGAAAAC ATGTTACGGC AAGTTCTGGA GCATTTGTCT 
    CAGCAAAGTG AAAGCCAGTA CCTAAAGATT CTAACAAGCC TTGCTGAAGT TGCTACAACA
    AATGGTCATA AACTGCTTAG CCTCTCTAGC AATTATGATG CTCAAATGAA GAGCCTTTTA
    AGGATTGTGA GAATGTTTTG TCACGTCTTT CGAATTGGTC CATCCTCCCC CAGTAATGGA
    ATTGATATGG GCTACAATGG GAATAAAACT CCAAGAAGCC AGGTGTTCAA GCCTCTGGAA
    TTGCTTTGGC ACTCGTTAGA TGAATGGCTA GTTTTAATAG CCACAGAATT GATGAAAAAC
    AAAAGAGACT CAACAGAGAT CACTTCTATT TTACTGAAAC AAAAAGGCCA AGATCAAGAT
    GCTGCTTCCA TTCCTCCATT TGAACCTCCA GGACCTGGGA GCTATGAAAA TCTGTCCACT
    GGCACAAGGG AATCTAAACC AGATGCTCTT GCAGGGAGAC AGGAAGCCAG TGCAGATTGT
    CAGGATGTTA TTTCTATGAC AGCTAACCGG CTAAGTGCTG TCATTCAAGC TTTTTACATG
    TGCTGTTCTT GTCAGATGCC TCCGGGAATG ACTTCACCTC GTTTCATTGA ATTTGTCTGC
    AAACATGATG AAGTTTTAAA ATGCTTTGTT AATAGAAATC CCAAAATTAT ATTTGACCAC
    TTTCACTTTC TCCTTGAATG TCCTGAGTTG ATGTCAAGAT TCATGCATAT CATAAAAGCA
    CAGCCTTTTA AAGATCGCTG TGAATGGTTC TATGAACATT TGCATTCAGG ACAGCCAGAT
    TCAGATATGG TGCACAGGCC AGTGAATGAA AATGATATCC TGCTGGTTCA CAGAGATTCT
    ATTTTTAGGA GTAGCTGTGA AGTTGTGTCA AAAGCAAATT GTGCAAAGCT AAAGCAAGGG
    ATTGCTGTAC GGTTCCATGG AGAAGAAGGC ATGGGTCAAG GTGTTGTGCG TGAGTGGTTT
    GATATTCTGT CCAATGAGAT AGTCAATCCT GATTATGCAT TGTTTACCCA GTCAGCTGAT
    GGAACAACTT TTCAGCCTAA TAGCAACTCT TATGTAAATC CTGATCACTT GAACTATTTT
    CGGTTTGCTG GGCAGATCTT GGGATTAGCG TTGAACCACA GGCAGCTGGT CAATATTTAC
    TTCACACGAT CCTTCTACAA GCACATTCTT GGTATTCCTG TAAATTACCA AGATGTGGCA
    TCCATTGATC CAGAATATGC GAAAAATTTG CAATGGATTT TAGATAATGA TATAAGTGAT
    CTGGGTCTAG AACTAACTTT TTCTGTTGAG ACTGATGTGT TTGGAGCAAT GGAAGAGGTG
    CCTTTGAAAC CTGGGGGTGG GAGTATTCTT GTGACACAAA ATAATAAAGC GGAGTACGTC
    CAGCTTGTTA CTGAACTTCG AATGACAAGA GCCATTCAGC CTCAGATCAA TGCTTTTTTA
    CAGGGCTTTC ATATGTTCAT TCCACCCTCC CTCATACAGC TTTTTGATGA ATATGAATTG
    GAGCTACTGC TTTCTGGCAT GCCAGAAATT GATGTGAGTG ATTGGATAAA AAATACAGAA
    TACACAAGTG GCTATGAAAG AGAAGATCCA GTTATTCAGT GGTTCTGGGA AGTTGTAGAA
    GACATTACTC AAGAGGAGAG AGTTCTTCTC TTACAGTTTG TTACGGGCAG TTCCAGGGTC
    CCACATGGTG GGTTTGCTAA TATCATGGGT GGAAGTGGAT TGCAAAACTT TACAATCGCT
    GCTGTGCCAT ATACTCCAAA TCTTTTACCA ACTTCAAGCA CATGCATCAA CATGCTCAAG
    TTACCTGAAT ACCCAAGTAA AGAAATACTC AAGGACAGAC TTCTTGTGGC ACTACATTGT
    GGCAGCTATG GTTACACAAT GGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu40894
    Clone ID Related Accession (Same CDS sequence) NM_001321083.2 , NM_001321083.1
    Accession Version NM_001321083.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2628bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-04
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HACE1 isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB094058.1, CN288032.1, BC034982.2, AL513472.9 and AL590402.10. On May 31, 2019 this sequence version replaced NM_001321083.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.42112.1, SRR1803614.223452.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCCAATGG TTATGGCTGA TCAACACAGG TCTGTTTCTG AACTACTATC AAATTCAAAA 
    TTTGATGTCA ATTATGCATT CGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT
    TGTGGATCGG TGGAATGCTT GGTTTTGCTG TTAAAGAAAG GAGCAAATCC TAACTATCAA
    GATATTTCAG GCTGTACACC CCTTCATTTG GCAGCAAGAA ATGGGCAGAA GAAATGTATG
    AGTAAATTAT TAGAATATAG CGCTGATGTC AACATTTGTA ATAATGAAGG CCTTACAGCA
    ATACATTGGC TGGCTGTGAA TGGGCGGACA GAACTACTCC ATGACCTTGT GCAGCATGTC
    AGTGATGTTG ATGTTGAGGA TGCCATGGGG CAGACAGCAC TGCATGTTGC CTGCCAGAAC
    GGTCACAAGA CGACAGTGCA GTGCTTGCTA GACAGTGGTG CTGATATTAA CAGGCCAAAT
    GTATCAGGAG CAACTCCATT GTACTTTGCT TGCAGTCATG GTCAGAGAGA TACAGCACAG
    ATCCTACTAT TACGAGGAGC CAAATATCTG CCAGATAAAA ATGGAGTAAC TCCTCTGGAT
    TTATGTGTAC AGGGTGGATA TGGAGAGACT TGTGAAGTAT TAATTCAATA TCACCCGAGG
    CTTTTTCAGA CTATTATTCA AATGACACAG AATGAAGACC TCCGAGAAAA CATGTTACGG
    CAAGTTCTGG AGCATTTGTC TCAGCAAAGT GAAAGCCAGT ACCTAAAGAT TCTAACAAGC
    CTTGCTGAAG TTGCTACAAC AAATGGTCAT AAACTGCTTA GCCTCTCTAG CAATTATGAT
    GCTCAAATGA AGAGCCTTTT AAGGATTGTG AGAATGTTTT GTCACGTCTT TCGAATTGGT
    CCATCCTCCC CCAGTAATGG AATTGATATG GGCTACAATG GGAATAAAAC TCCAAGAAGC
    CAGGTGTTCA AGCCTCTGGA ATTGCTTTGG CACTCGTTAG ATGAATGGCT AGTTTTAATA
    GCCACAGAAT TGATGAAAAA CAAAAGAGAC TCAACAGAGA TCACTTCTAT TTTACTGAAA
    CAAAAAGGCC AAGATCAAGA TGCTGCTTCC ATTCCTCCAT TTGAACCTCC AGGACCTGGG
    AGCTATGAAA ATCTGTCCAC TGGCACAAGG GAATCTAAAC CAGATGCTCT TGCAGGGAGA
    CAGGAAGCCA GTGCAGATTG TCAGGATGTT ATTTCTATGA CAGCTAACCG GCTAAGTGCT
    GTCATTCAAG CTTTTTACAT GTGCTGTTCT TGTCAGATGC CTCCGGGAAT GACTTCACCT
    CGTTTCATTG AATTTGTCTG CAAACATGAT GAAGTTTTAA AATGCTTTGT TAATAGAAAT
    CCCAAAATTA TATTTGACCA CTTTCACTTT CTCCTTGAAT GTCCTGAGTT GATGTCAAGA
    TTCATGCATA TCATAAAAGC ACAGCCTTTT AAAGATCGCT GTGAATGGTT CTATGAACAT
    TTGCATTCAG GACAGCCAGA TTCAGATATG GTGCACAGGC CAGTGAATGA AAATGATATC
    CTGCTGGTTC ACAGAGATTC TATTTTTAGG AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT
    TGTGCAAAGC TAAAGCAAGG GATTGCTGTA CGGTTCCATG GAGAAGAAGG CATGGGTCAA
    GGTGTTGTGC GTGAGTGGTT TGATATTCTG TCCAATGAGA TAGTCAATCC TGATTATGCA
    TTGTTTACCC AGTCAGCTGA TGGAACAACT TTTCAGCCTA ATAGCAACTC TTATGTAAAT
    CCTGATCACT TGAACTATTT TCGGTTTGCT GGGCAGATCT TGGGATTAGC GTTGAACCAC
    AGGCAGCTGG TCAATATTTA CTTCACACGA TCCTTCTACA AGCACATTCT TGGTATTCCT
    GTAAATTACC AAGATGTGGC ATCCATTGAT CCAGAATATG CGAAAAATTT GCAATGGATT
    TTAGATAATG ATATAAGTGA TCTGGGTCTA GAACTAACTT TTTCTGTTGA GACTGATGTG
    TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA CCTGGGGGTG GGAGTATTCT TGTGACACAA
    AATAATAAAG CGGAGTACGT CCAGCTTGTT ACTGAACTTC GAATGACAAG AGCCATTCAG
    CCTCAGATCA ATGCTTTTTT ACAGGGCTTT CATATGTTCA TTCCACCCTC CCTCATACAG
    CTTTTTGATG AATATGAATT GGAGCTACTG CTTTCTGGCA TGCCAGAAAT TGATGTGAGT
    GATTGGATAA AAAATACAGA ATACACAAGT GGCTATGAAA GAGAAGATCC AGTTATTCAG
    TGGTTCTGGG AAGTTGTAGA AGACATTACT CAAGAGGAGA GAGTTCTTCT CTTACAGTTT
    GTTACGGGCA GTTCCAGGGT CCCACATGGT GGGTTTGCTA ATATCATGGG TGGAAGTGGA
    TTGCAAAACT TTACAATCGC TGCTGTGCCA TATACTCCAA ATCTTTTACC AACTTCAAGC
    ACATGCATCA ACATGCTCAA GTTACCTGAA TACCCAAGTA AAGAAATACT CAAGGACAGA
    CTTCTTGTGG CACTACATTG TGGCAGCTAT GGTTACACAA TGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001308012.1
    CDS195..2822
    Translation

    Target ORF information:

    RefSeq Version NM_001321083.2
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001321083.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCCAATGG TTATGGCTGA TCAACACAGG TCTGTTTCTG AACTACTATC AAATTCAAAA 
    TTTGATGTCA ATTATGCATT CGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT
    TGTGGATCGG TGGAATGCTT GGTTTTGCTG TTAAAGAAAG GAGCAAATCC TAACTATCAA
    GATATTTCAG GCTGTACACC CCTTCATTTG GCAGCAAGAA ATGGGCAGAA GAAATGTATG
    AGTAAATTAT TAGAATATAG CGCTGATGTC AACATTTGTA ATAATGAAGG CCTTACAGCA
    ATACATTGGC TGGCTGTGAA TGGGCGGACA GAACTACTCC ATGACCTTGT GCAGCATGTC
    AGTGATGTTG ATGTTGAGGA TGCCATGGGG CAGACAGCAC TGCATGTTGC CTGCCAGAAC
    GGTCACAAGA CGACAGTGCA GTGCTTGCTA GACAGTGGTG CTGATATTAA CAGGCCAAAT
    GTATCAGGAG CAACTCCATT GTACTTTGCT TGCAGTCATG GTCAGAGAGA TACAGCACAG
    ATCCTACTAT TACGAGGAGC CAAATATCTG CCAGATAAAA ATGGAGTAAC TCCTCTGGAT
    TTATGTGTAC AGGGTGGATA TGGAGAGACT TGTGAAGTAT TAATTCAATA TCACCCGAGG
    CTTTTTCAGA CTATTATTCA AATGACACAG AATGAAGACC TCCGAGAAAA CATGTTACGG
    CAAGTTCTGG AGCATTTGTC TCAGCAAAGT GAAAGCCAGT ACCTAAAGAT TCTAACAAGC
    CTTGCTGAAG TTGCTACAAC AAATGGTCAT AAACTGCTTA GCCTCTCTAG CAATTATGAT
    GCTCAAATGA AGAGCCTTTT AAGGATTGTG AGAATGTTTT GTCACGTCTT TCGAATTGGT
    CCATCCTCCC CCAGTAATGG AATTGATATG GGCTACAATG GGAATAAAAC TCCAAGAAGC
    CAGGTGTTCA AGCCTCTGGA ATTGCTTTGG CACTCGTTAG ATGAATGGCT AGTTTTAATA
    GCCACAGAAT TGATGAAAAA CAAAAGAGAC TCAACAGAGA TCACTTCTAT TTTACTGAAA
    CAAAAAGGCC AAGATCAAGA TGCTGCTTCC ATTCCTCCAT TTGAACCTCC AGGACCTGGG
    AGCTATGAAA ATCTGTCCAC TGGCACAAGG GAATCTAAAC CAGATGCTCT TGCAGGGAGA
    CAGGAAGCCA GTGCAGATTG TCAGGATGTT ATTTCTATGA CAGCTAACCG GCTAAGTGCT
    GTCATTCAAG CTTTTTACAT GTGCTGTTCT TGTCAGATGC CTCCGGGAAT GACTTCACCT
    CGTTTCATTG AATTTGTCTG CAAACATGAT GAAGTTTTAA AATGCTTTGT TAATAGAAAT
    CCCAAAATTA TATTTGACCA CTTTCACTTT CTCCTTGAAT GTCCTGAGTT GATGTCAAGA
    TTCATGCATA TCATAAAAGC ACAGCCTTTT AAAGATCGCT GTGAATGGTT CTATGAACAT
    TTGCATTCAG GACAGCCAGA TTCAGATATG GTGCACAGGC CAGTGAATGA AAATGATATC
    CTGCTGGTTC ACAGAGATTC TATTTTTAGG AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT
    TGTGCAAAGC TAAAGCAAGG GATTGCTGTA CGGTTCCATG GAGAAGAAGG CATGGGTCAA
    GGTGTTGTGC GTGAGTGGTT TGATATTCTG TCCAATGAGA TAGTCAATCC TGATTATGCA
    TTGTTTACCC AGTCAGCTGA TGGAACAACT TTTCAGCCTA ATAGCAACTC TTATGTAAAT
    CCTGATCACT TGAACTATTT TCGGTTTGCT GGGCAGATCT TGGGATTAGC GTTGAACCAC
    AGGCAGCTGG TCAATATTTA CTTCACACGA TCCTTCTACA AGCACATTCT TGGTATTCCT
    GTAAATTACC AAGATGTGGC ATCCATTGAT CCAGAATATG CGAAAAATTT GCAATGGATT
    TTAGATAATG ATATAAGTGA TCTGGGTCTA GAACTAACTT TTTCTGTTGA GACTGATGTG
    TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA CCTGGGGGTG GGAGTATTCT TGTGACACAA
    AATAATAAAG CGGAGTACGT CCAGCTTGTT ACTGAACTTC GAATGACAAG AGCCATTCAG
    CCTCAGATCA ATGCTTTTTT ACAGGGCTTT CATATGTTCA TTCCACCCTC CCTCATACAG
    CTTTTTGATG AATATGAATT GGAGCTACTG CTTTCTGGCA TGCCAGAAAT TGATGTGAGT
    GATTGGATAA AAAATACAGA ATACACAAGT GGCTATGAAA GAGAAGATCC AGTTATTCAG
    TGGTTCTGGG AAGTTGTAGA AGACATTACT CAAGAGGAGA GAGTTCTTCT CTTACAGTTT
    GTTACGGGCA GTTCCAGGGT CCCACATGGT GGGTTTGCTA ATATCATGGG TGGAAGTGGA
    TTGCAAAACT TTACAATCGC TGCTGTGCCA TATACTCCAA ATCTTTTACC AACTTCAAGC
    ACATGCATCA ACATGCTCAA GTTACCTGAA TACCCAAGTA AAGAAATACT CAAGGACAGA
    CTTCTTGTGG CACTACATTG TGGCAGCTAT GGTTACACAA TGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu40894
    Clone ID Related Accession (Same CDS sequence) NM_001321083.2 , NM_001321083.1
    Accession Version NM_001321083.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2628bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-22
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HACE1 isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB094058.1, CN288032.1, BC034982.2, AL513472.9 and AL590402.10. On Mar 18, 2016 this sequence version replaced XM_006715528.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.42112.1, SRR1803614.223452.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCCAATGG TTATGGCTGA TCAACACAGG TCTGTTTCTG AACTACTATC AAATTCAAAA 
    TTTGATGTCA ATTATGCATT CGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT
    TGTGGATCGG TGGAATGCTT GGTTTTGCTG TTAAAGAAAG GAGCAAATCC TAACTATCAA
    GATATTTCAG GCTGTACACC CCTTCATTTG GCAGCAAGAA ATGGGCAGAA GAAATGTATG
    AGTAAATTAT TAGAATATAG CGCTGATGTC AACATTTGTA ATAATGAAGG CCTTACAGCA
    ATACATTGGC TGGCTGTGAA TGGGCGGACA GAACTACTCC ATGACCTTGT GCAGCATGTC
    AGTGATGTTG ATGTTGAGGA TGCCATGGGG CAGACAGCAC TGCATGTTGC CTGCCAGAAC
    GGTCACAAGA CGACAGTGCA GTGCTTGCTA GACAGTGGTG CTGATATTAA CAGGCCAAAT
    GTATCAGGAG CAACTCCATT GTACTTTGCT TGCAGTCATG GTCAGAGAGA TACAGCACAG
    ATCCTACTAT TACGAGGAGC CAAATATCTG CCAGATAAAA ATGGAGTAAC TCCTCTGGAT
    TTATGTGTAC AGGGTGGATA TGGAGAGACT TGTGAAGTAT TAATTCAATA TCACCCGAGG
    CTTTTTCAGA CTATTATTCA AATGACACAG AATGAAGACC TCCGAGAAAA CATGTTACGG
    CAAGTTCTGG AGCATTTGTC TCAGCAAAGT GAAAGCCAGT ACCTAAAGAT TCTAACAAGC
    CTTGCTGAAG TTGCTACAAC AAATGGTCAT AAACTGCTTA GCCTCTCTAG CAATTATGAT
    GCTCAAATGA AGAGCCTTTT AAGGATTGTG AGAATGTTTT GTCACGTCTT TCGAATTGGT
    CCATCCTCCC CCAGTAATGG AATTGATATG GGCTACAATG GGAATAAAAC TCCAAGAAGC
    CAGGTGTTCA AGCCTCTGGA ATTGCTTTGG CACTCGTTAG ATGAATGGCT AGTTTTAATA
    GCCACAGAAT TGATGAAAAA CAAAAGAGAC TCAACAGAGA TCACTTCTAT TTTACTGAAA
    CAAAAAGGCC AAGATCAAGA TGCTGCTTCC ATTCCTCCAT TTGAACCTCC AGGACCTGGG
    AGCTATGAAA ATCTGTCCAC TGGCACAAGG GAATCTAAAC CAGATGCTCT TGCAGGGAGA
    CAGGAAGCCA GTGCAGATTG TCAGGATGTT ATTTCTATGA CAGCTAACCG GCTAAGTGCT
    GTCATTCAAG CTTTTTACAT GTGCTGTTCT TGTCAGATGC CTCCGGGAAT GACTTCACCT
    CGTTTCATTG AATTTGTCTG CAAACATGAT GAAGTTTTAA AATGCTTTGT TAATAGAAAT
    CCCAAAATTA TATTTGACCA CTTTCACTTT CTCCTTGAAT GTCCTGAGTT GATGTCAAGA
    TTCATGCATA TCATAAAAGC ACAGCCTTTT AAAGATCGCT GTGAATGGTT CTATGAACAT
    TTGCATTCAG GACAGCCAGA TTCAGATATG GTGCACAGGC CAGTGAATGA AAATGATATC
    CTGCTGGTTC ACAGAGATTC TATTTTTAGG AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT
    TGTGCAAAGC TAAAGCAAGG GATTGCTGTA CGGTTCCATG GAGAAGAAGG CATGGGTCAA
    GGTGTTGTGC GTGAGTGGTT TGATATTCTG TCCAATGAGA TAGTCAATCC TGATTATGCA
    TTGTTTACCC AGTCAGCTGA TGGAACAACT TTTCAGCCTA ATAGCAACTC TTATGTAAAT
    CCTGATCACT TGAACTATTT TCGGTTTGCT GGGCAGATCT TGGGATTAGC GTTGAACCAC
    AGGCAGCTGG TCAATATTTA CTTCACACGA TCCTTCTACA AGCACATTCT TGGTATTCCT
    GTAAATTACC AAGATGTGGC ATCCATTGAT CCAGAATATG CGAAAAATTT GCAATGGATT
    TTAGATAATG ATATAAGTGA TCTGGGTCTA GAACTAACTT TTTCTGTTGA GACTGATGTG
    TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA CCTGGGGGTG GGAGTATTCT TGTGACACAA
    AATAATAAAG CGGAGTACGT CCAGCTTGTT ACTGAACTTC GAATGACAAG AGCCATTCAG
    CCTCAGATCA ATGCTTTTTT ACAGGGCTTT CATATGTTCA TTCCACCCTC CCTCATACAG
    CTTTTTGATG AATATGAATT GGAGCTACTG CTTTCTGGCA TGCCAGAAAT TGATGTGAGT
    GATTGGATAA AAAATACAGA ATACACAAGT GGCTATGAAA GAGAAGATCC AGTTATTCAG
    TGGTTCTGGG AAGTTGTAGA AGACATTACT CAAGAGGAGA GAGTTCTTCT CTTACAGTTT
    GTTACGGGCA GTTCCAGGGT CCCACATGGT GGGTTTGCTA ATATCATGGG TGGAAGTGGA
    TTGCAAAACT TTACAATCGC TGCTGTGCCA TATACTCCAA ATCTTTTACC AACTTCAAGC
    ACATGCATCA ACATGCTCAA GTTACCTGAA TACCCAAGTA AAGAAATACT CAAGGACAGA
    CTTCTTGTGG CACTACATTG TGGCAGCTAT GGTTACACAA TGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001308012.1
    CDS195..2822
    Translation

    Target ORF information:

    RefSeq Version NM_001321083.1
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001321083.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCCAATGG TTATGGCTGA TCAACACAGG TCTGTTTCTG AACTACTATC AAATTCAAAA 
    TTTGATGTCA ATTATGCATT CGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT
    TGTGGATCGG TGGAATGCTT GGTTTTGCTG TTAAAGAAAG GAGCAAATCC TAACTATCAA
    GATATTTCAG GCTGTACACC CCTTCATTTG GCAGCAAGAA ATGGGCAGAA GAAATGTATG
    AGTAAATTAT TAGAATATAG CGCTGATGTC AACATTTGTA ATAATGAAGG CCTTACAGCA
    ATACATTGGC TGGCTGTGAA TGGGCGGACA GAACTACTCC ATGACCTTGT GCAGCATGTC
    AGTGATGTTG ATGTTGAGGA TGCCATGGGG CAGACAGCAC TGCATGTTGC CTGCCAGAAC
    GGTCACAAGA CGACAGTGCA GTGCTTGCTA GACAGTGGTG CTGATATTAA CAGGCCAAAT
    GTATCAGGAG CAACTCCATT GTACTTTGCT TGCAGTCATG GTCAGAGAGA TACAGCACAG
    ATCCTACTAT TACGAGGAGC CAAATATCTG CCAGATAAAA ATGGAGTAAC TCCTCTGGAT
    TTATGTGTAC AGGGTGGATA TGGAGAGACT TGTGAAGTAT TAATTCAATA TCACCCGAGG
    CTTTTTCAGA CTATTATTCA AATGACACAG AATGAAGACC TCCGAGAAAA CATGTTACGG
    CAAGTTCTGG AGCATTTGTC TCAGCAAAGT GAAAGCCAGT ACCTAAAGAT TCTAACAAGC
    CTTGCTGAAG TTGCTACAAC AAATGGTCAT AAACTGCTTA GCCTCTCTAG CAATTATGAT
    GCTCAAATGA AGAGCCTTTT AAGGATTGTG AGAATGTTTT GTCACGTCTT TCGAATTGGT
    CCATCCTCCC CCAGTAATGG AATTGATATG GGCTACAATG GGAATAAAAC TCCAAGAAGC
    CAGGTGTTCA AGCCTCTGGA ATTGCTTTGG CACTCGTTAG ATGAATGGCT AGTTTTAATA
    GCCACAGAAT TGATGAAAAA CAAAAGAGAC TCAACAGAGA TCACTTCTAT TTTACTGAAA
    CAAAAAGGCC AAGATCAAGA TGCTGCTTCC ATTCCTCCAT TTGAACCTCC AGGACCTGGG
    AGCTATGAAA ATCTGTCCAC TGGCACAAGG GAATCTAAAC CAGATGCTCT TGCAGGGAGA
    CAGGAAGCCA GTGCAGATTG TCAGGATGTT ATTTCTATGA CAGCTAACCG GCTAAGTGCT
    GTCATTCAAG CTTTTTACAT GTGCTGTTCT TGTCAGATGC CTCCGGGAAT GACTTCACCT
    CGTTTCATTG AATTTGTCTG CAAACATGAT GAAGTTTTAA AATGCTTTGT TAATAGAAAT
    CCCAAAATTA TATTTGACCA CTTTCACTTT CTCCTTGAAT GTCCTGAGTT GATGTCAAGA
    TTCATGCATA TCATAAAAGC ACAGCCTTTT AAAGATCGCT GTGAATGGTT CTATGAACAT
    TTGCATTCAG GACAGCCAGA TTCAGATATG GTGCACAGGC CAGTGAATGA AAATGATATC
    CTGCTGGTTC ACAGAGATTC TATTTTTAGG AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT
    TGTGCAAAGC TAAAGCAAGG GATTGCTGTA CGGTTCCATG GAGAAGAAGG CATGGGTCAA
    GGTGTTGTGC GTGAGTGGTT TGATATTCTG TCCAATGAGA TAGTCAATCC TGATTATGCA
    TTGTTTACCC AGTCAGCTGA TGGAACAACT TTTCAGCCTA ATAGCAACTC TTATGTAAAT
    CCTGATCACT TGAACTATTT TCGGTTTGCT GGGCAGATCT TGGGATTAGC GTTGAACCAC
    AGGCAGCTGG TCAATATTTA CTTCACACGA TCCTTCTACA AGCACATTCT TGGTATTCCT
    GTAAATTACC AAGATGTGGC ATCCATTGAT CCAGAATATG CGAAAAATTT GCAATGGATT
    TTAGATAATG ATATAAGTGA TCTGGGTCTA GAACTAACTT TTTCTGTTGA GACTGATGTG
    TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA CCTGGGGGTG GGAGTATTCT TGTGACACAA
    AATAATAAAG CGGAGTACGT CCAGCTTGTT ACTGAACTTC GAATGACAAG AGCCATTCAG
    CCTCAGATCA ATGCTTTTTT ACAGGGCTTT CATATGTTCA TTCCACCCTC CCTCATACAG
    CTTTTTGATG AATATGAATT GGAGCTACTG CTTTCTGGCA TGCCAGAAAT TGATGTGAGT
    GATTGGATAA AAAATACAGA ATACACAAGT GGCTATGAAA GAGAAGATCC AGTTATTCAG
    TGGTTCTGGG AAGTTGTAGA AGACATTACT CAAGAGGAGA GAGTTCTTCT CTTACAGTTT
    GTTACGGGCA GTTCCAGGGT CCCACATGGT GGGTTTGCTA ATATCATGGG TGGAAGTGGA
    TTGCAAAACT TTACAATCGC TGCTGTGCCA TATACTCCAA ATCTTTTACC AACTTCAAGC
    ACATGCATCA ACATGCTCAA GTTACCTGAA TACCCAAGTA AAGAAATACT CAAGGACAGA
    CTTCTTGTGG CACTACATTG TGGCAGCTAT GGTTACACAA TGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu40895
    Clone ID Related Accession (Same CDS sequence) NM_001321080.1 , NM_001321080.2
    Accession Version NM_001321080.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2598bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-22
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HACE1 isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK131207.1, BC034982.2, AL513472.9 and AL590402.10. On Mar 18, 2016 this sequence version replaced XM_006715529.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.183999.1, SRR1660807.22692.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGAGAG CGATGGAGCA ACTCAACCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGTTGC CCGAGGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTCGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC GGTGGAATGC
    TTGGTTTTGC TGTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGCTGTACA
    CCCCTTCATT TGGCAGCAAG AAATGGGCAG AAGAAATGTA TGAGTAAATT ATTAGAATAT
    AGCGCTGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAACAGTGCA GTGCTTGCTA
    GACAGTGGTG CTGATATTAA CAGGCCAAAT GTATCAGGAG CAACTCCATT GTACTTTGCT
    TGCAGTCATG GTCAGAGAGA TACAGCACAG ATCCTACTAT TACGAGGAGC CAAATATCTG
    CCAGATAAAA ATGGAGTAAC TCCTCTGGAT TTATGTGTAC AGGGTGGATA TGGAGAGACT
    TGTGAAGTAT TAATTCAATA TCACCCGAGG CTTTTTCAGA CTATTATTCA AATGACACAG
    AATGAAGACC TCCGAGAAAA CATGTTACGG CAAGTTCTGG AGCATTTGTC TCAGCAAAGT
    GAAAGCCAGT ACCTAAAGAT TCTAACAAGC CTTGCTGAAG TTGCTACAAC AAATGGTCAT
    AAACTGCTTA GCCTCTCTAG CAATTATGAT GCTCAAATGA AGAGCCTTTT AAGGATTGTG
    AGAATGTTTT GTCACGTCTT TCGAATTGGT CCATCCTCCC CCAGTAATGG AATTGATATG
    GGCTACAATG GGAATAAAAC TCCAAGAAGC CAGGTGTTCA AGCCTCTGGA ATTGCTTTGG
    CACTCGTTAG ATGAATGGCT AGTTTTAATA GCCACAGAAT TGATGAAAAA CAAAAGAGAC
    TCAACAGAGA TCACTTCTAT TTTACTGAAA CAAAAAGGCC AAGATCAAGA TGCTGCTTCC
    ATTCCTCCAT TTGAACCTCC AGGACCTGGG AGCTATGAAA ATCTGTCCAC TGGCACAAGG
    GAATCTAAAC CAGATGCTCT TGCAGGGAGA CAGGAAGCCA GTGCAGATTG TCAGGATGTT
    ATTTCTATGA CAGCTAACCG GCTAAGTGCT GTCATTCAAG CTTTTTACAT GTGCTGTTCT
    TGTCAGATGC CTCCGGGAAT GACTTCACCT CGTTTCATTG AATTTGTCTG CAAACATGAT
    GAAGTTTTAA AATGCTTTGT TAATAGAAAT CCCAAAATTA TATTTGACCA CTTTCACTTT
    CTCCTTGAAT GTCCTGAGTT GATGTCAAGA TTCATGCATA TCATAAAAGC ACAGCCTTTT
    AAAGATCGCT GTGAATGGTT CTATGAACAT TTGCATTCAG GACAGCCAGA TTCAGATATG
    GTGCACAGGC CAGTGAATGA AAATGATATC CTGCTGGTTC ACAGAGATTC TATTTTTAGG
    AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT TGTGCAAAGC TAAAGCAAGG GATTGCTGTA
    CGGTTCCATG GAGAAGAAGG CATGGGTCAA GGTGTTGTGC GTGAGTGGTT TGATATTCTG
    TCCAATGAGA TAGTCAATCC TGATTATGCA TTGTTTACCC AGTCAGCTGA TGGAACAACT
    TTTCAGCCTA ATAGCAACTC TTATGTAAAT CCTGATCACT TGAACTATTT TCGGTTTGCT
    GGGCAGATCT TGGGATTAGC GTTGAACCAC AGGCAGCTGG TCAATATTTA CTTCACACGA
    TCCTTCTACA AGCACATTCT TGGTATTCCT GTAAATTACC AAGATGTGGC ATCCATTGAT
    CCAGAATATG CGAAAAATTT GCAATGGATT TTAGATAATG ATATAAGTGA TCTGGGTCTA
    GAACTAACTT TTTCTGTTGA GACTGATGTG TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA
    CCTGGGGGTG GGAGTATTCT TGTGACACAA AATAATAAAG CGGAGTACGT CCAGCTTGTT
    ACTGAACTTC GAATGACAAG AGCCATTCAG CCTCAGATCA ATGCTTTTTT ACAGGGCTTT
    CATATGTTCA TTCCACCCTC CCTCATACAG CTTTTTGATG AATATGAATT GGAGCTACTG
    CTTTCTGGCA TGCCAGAAAT TGATGTGAGT GATTGGATAA AAAATACAGA ATACACAAGT
    GGCTATGAAA GAGAAGATCC AGTTATTCAG TGGTTCTGGG AAGTTGTAGA AGACATTACT
    CAAGAGGAGA GAGTTCTTCT CTTACAGTTT GTTACGGGCA GTTCCAGGGT CCCACATGGT
    GGGTTTGCTA ATATCATGGG TGGAAGTGGA TTGCAAAACT TTACAATCGC TGCTGTGCCA
    TATACTCCAA ATCTTTTACC AACTTCAAGC ACATGCATCA ACATGCTCAA GTTACCTGAA
    TACCCAAGTA AAGAAATACT CAAGGACAGA CTTCTTGTGG CACTACATTG TGGCAGCTAT
    GGTTACACAA TGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001308009.1
    CDS278..2875
    Translation

    Target ORF information:

    RefSeq Version NM_001321080.1
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001321080.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGAGAG CGATGGAGCA ACTCAACCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGTTGC CCGAGGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTCGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC GGTGGAATGC
    TTGGTTTTGC TGTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGCTGTACA
    CCCCTTCATT TGGCAGCAAG AAATGGGCAG AAGAAATGTA TGAGTAAATT ATTAGAATAT
    AGCGCTGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAACAGTGCA GTGCTTGCTA
    GACAGTGGTG CTGATATTAA CAGGCCAAAT GTATCAGGAG CAACTCCATT GTACTTTGCT
    TGCAGTCATG GTCAGAGAGA TACAGCACAG ATCCTACTAT TACGAGGAGC CAAATATCTG
    CCAGATAAAA ATGGAGTAAC TCCTCTGGAT TTATGTGTAC AGGGTGGATA TGGAGAGACT
    TGTGAAGTAT TAATTCAATA TCACCCGAGG CTTTTTCAGA CTATTATTCA AATGACACAG
    AATGAAGACC TCCGAGAAAA CATGTTACGG CAAGTTCTGG AGCATTTGTC TCAGCAAAGT
    GAAAGCCAGT ACCTAAAGAT TCTAACAAGC CTTGCTGAAG TTGCTACAAC AAATGGTCAT
    AAACTGCTTA GCCTCTCTAG CAATTATGAT GCTCAAATGA AGAGCCTTTT AAGGATTGTG
    AGAATGTTTT GTCACGTCTT TCGAATTGGT CCATCCTCCC CCAGTAATGG AATTGATATG
    GGCTACAATG GGAATAAAAC TCCAAGAAGC CAGGTGTTCA AGCCTCTGGA ATTGCTTTGG
    CACTCGTTAG ATGAATGGCT AGTTTTAATA GCCACAGAAT TGATGAAAAA CAAAAGAGAC
    TCAACAGAGA TCACTTCTAT TTTACTGAAA CAAAAAGGCC AAGATCAAGA TGCTGCTTCC
    ATTCCTCCAT TTGAACCTCC AGGACCTGGG AGCTATGAAA ATCTGTCCAC TGGCACAAGG
    GAATCTAAAC CAGATGCTCT TGCAGGGAGA CAGGAAGCCA GTGCAGATTG TCAGGATGTT
    ATTTCTATGA CAGCTAACCG GCTAAGTGCT GTCATTCAAG CTTTTTACAT GTGCTGTTCT
    TGTCAGATGC CTCCGGGAAT GACTTCACCT CGTTTCATTG AATTTGTCTG CAAACATGAT
    GAAGTTTTAA AATGCTTTGT TAATAGAAAT CCCAAAATTA TATTTGACCA CTTTCACTTT
    CTCCTTGAAT GTCCTGAGTT GATGTCAAGA TTCATGCATA TCATAAAAGC ACAGCCTTTT
    AAAGATCGCT GTGAATGGTT CTATGAACAT TTGCATTCAG GACAGCCAGA TTCAGATATG
    GTGCACAGGC CAGTGAATGA AAATGATATC CTGCTGGTTC ACAGAGATTC TATTTTTAGG
    AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT TGTGCAAAGC TAAAGCAAGG GATTGCTGTA
    CGGTTCCATG GAGAAGAAGG CATGGGTCAA GGTGTTGTGC GTGAGTGGTT TGATATTCTG
    TCCAATGAGA TAGTCAATCC TGATTATGCA TTGTTTACCC AGTCAGCTGA TGGAACAACT
    TTTCAGCCTA ATAGCAACTC TTATGTAAAT CCTGATCACT TGAACTATTT TCGGTTTGCT
    GGGCAGATCT TGGGATTAGC GTTGAACCAC AGGCAGCTGG TCAATATTTA CTTCACACGA
    TCCTTCTACA AGCACATTCT TGGTATTCCT GTAAATTACC AAGATGTGGC ATCCATTGAT
    CCAGAATATG CGAAAAATTT GCAATGGATT TTAGATAATG ATATAAGTGA TCTGGGTCTA
    GAACTAACTT TTTCTGTTGA GACTGATGTG TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA
    CCTGGGGGTG GGAGTATTCT TGTGACACAA AATAATAAAG CGGAGTACGT CCAGCTTGTT
    ACTGAACTTC GAATGACAAG AGCCATTCAG CCTCAGATCA ATGCTTTTTT ACAGGGCTTT
    CATATGTTCA TTCCACCCTC CCTCATACAG CTTTTTGATG AATATGAATT GGAGCTACTG
    CTTTCTGGCA TGCCAGAAAT TGATGTGAGT GATTGGATAA AAAATACAGA ATACACAAGT
    GGCTATGAAA GAGAAGATCC AGTTATTCAG TGGTTCTGGG AAGTTGTAGA AGACATTACT
    CAAGAGGAGA GAGTTCTTCT CTTACAGTTT GTTACGGGCA GTTCCAGGGT CCCACATGGT
    GGGTTTGCTA ATATCATGGG TGGAAGTGGA TTGCAAAACT TTACAATCGC TGCTGTGCCA
    TATACTCCAA ATCTTTTACC AACTTCAAGC ACATGCATCA ACATGCTCAA GTTACCTGAA
    TACCCAAGTA AAGAAATACT CAAGGACAGA CTTCTTGTGG CACTACATTG TGGCAGCTAT
    GGTTACACAA TGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu40895
    Clone ID Related Accession (Same CDS sequence) NM_001321080.1 , NM_001321080.2
    Accession Version NM_001321080.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2598bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-04
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HACE1 isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK131207.1, BC034982.2, AL513472.9 and AL590402.10. On Jun 1, 2019 this sequence version replaced NM_001321080.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.22692.1, SRR1803617.253935.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGAGAG CGATGGAGCA ACTCAACCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGTTGC CCGAGGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTCGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC GGTGGAATGC
    TTGGTTTTGC TGTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGCTGTACA
    CCCCTTCATT TGGCAGCAAG AAATGGGCAG AAGAAATGTA TGAGTAAATT ATTAGAATAT
    AGCGCTGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAACAGTGCA GTGCTTGCTA
    GACAGTGGTG CTGATATTAA CAGGCCAAAT GTATCAGGAG CAACTCCATT GTACTTTGCT
    TGCAGTCATG GTCAGAGAGA TACAGCACAG ATCCTACTAT TACGAGGAGC CAAATATCTG
    CCAGATAAAA ATGGAGTAAC TCCTCTGGAT TTATGTGTAC AGGGTGGATA TGGAGAGACT
    TGTGAAGTAT TAATTCAATA TCACCCGAGG CTTTTTCAGA CTATTATTCA AATGACACAG
    AATGAAGACC TCCGAGAAAA CATGTTACGG CAAGTTCTGG AGCATTTGTC TCAGCAAAGT
    GAAAGCCAGT ACCTAAAGAT TCTAACAAGC CTTGCTGAAG TTGCTACAAC AAATGGTCAT
    AAACTGCTTA GCCTCTCTAG CAATTATGAT GCTCAAATGA AGAGCCTTTT AAGGATTGTG
    AGAATGTTTT GTCACGTCTT TCGAATTGGT CCATCCTCCC CCAGTAATGG AATTGATATG
    GGCTACAATG GGAATAAAAC TCCAAGAAGC CAGGTGTTCA AGCCTCTGGA ATTGCTTTGG
    CACTCGTTAG ATGAATGGCT AGTTTTAATA GCCACAGAAT TGATGAAAAA CAAAAGAGAC
    TCAACAGAGA TCACTTCTAT TTTACTGAAA CAAAAAGGCC AAGATCAAGA TGCTGCTTCC
    ATTCCTCCAT TTGAACCTCC AGGACCTGGG AGCTATGAAA ATCTGTCCAC TGGCACAAGG
    GAATCTAAAC CAGATGCTCT TGCAGGGAGA CAGGAAGCCA GTGCAGATTG TCAGGATGTT
    ATTTCTATGA CAGCTAACCG GCTAAGTGCT GTCATTCAAG CTTTTTACAT GTGCTGTTCT
    TGTCAGATGC CTCCGGGAAT GACTTCACCT CGTTTCATTG AATTTGTCTG CAAACATGAT
    GAAGTTTTAA AATGCTTTGT TAATAGAAAT CCCAAAATTA TATTTGACCA CTTTCACTTT
    CTCCTTGAAT GTCCTGAGTT GATGTCAAGA TTCATGCATA TCATAAAAGC ACAGCCTTTT
    AAAGATCGCT GTGAATGGTT CTATGAACAT TTGCATTCAG GACAGCCAGA TTCAGATATG
    GTGCACAGGC CAGTGAATGA AAATGATATC CTGCTGGTTC ACAGAGATTC TATTTTTAGG
    AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT TGTGCAAAGC TAAAGCAAGG GATTGCTGTA
    CGGTTCCATG GAGAAGAAGG CATGGGTCAA GGTGTTGTGC GTGAGTGGTT TGATATTCTG
    TCCAATGAGA TAGTCAATCC TGATTATGCA TTGTTTACCC AGTCAGCTGA TGGAACAACT
    TTTCAGCCTA ATAGCAACTC TTATGTAAAT CCTGATCACT TGAACTATTT TCGGTTTGCT
    GGGCAGATCT TGGGATTAGC GTTGAACCAC AGGCAGCTGG TCAATATTTA CTTCACACGA
    TCCTTCTACA AGCACATTCT TGGTATTCCT GTAAATTACC AAGATGTGGC ATCCATTGAT
    CCAGAATATG CGAAAAATTT GCAATGGATT TTAGATAATG ATATAAGTGA TCTGGGTCTA
    GAACTAACTT TTTCTGTTGA GACTGATGTG TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA
    CCTGGGGGTG GGAGTATTCT TGTGACACAA AATAATAAAG CGGAGTACGT CCAGCTTGTT
    ACTGAACTTC GAATGACAAG AGCCATTCAG CCTCAGATCA ATGCTTTTTT ACAGGGCTTT
    CATATGTTCA TTCCACCCTC CCTCATACAG CTTTTTGATG AATATGAATT GGAGCTACTG
    CTTTCTGGCA TGCCAGAAAT TGATGTGAGT GATTGGATAA AAAATACAGA ATACACAAGT
    GGCTATGAAA GAGAAGATCC AGTTATTCAG TGGTTCTGGG AAGTTGTAGA AGACATTACT
    CAAGAGGAGA GAGTTCTTCT CTTACAGTTT GTTACGGGCA GTTCCAGGGT CCCACATGGT
    GGGTTTGCTA ATATCATGGG TGGAAGTGGA TTGCAAAACT TTACAATCGC TGCTGTGCCA
    TATACTCCAA ATCTTTTACC AACTTCAAGC ACATGCATCA ACATGCTCAA GTTACCTGAA
    TACCCAAGTA AAGAAATACT CAAGGACAGA CTTCTTGTGG CACTACATTG TGGCAGCTAT
    GGTTACACAA TGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001308009.1
    CDS278..2875
    Translation

    Target ORF information:

    RefSeq Version NM_001321080.2
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001321080.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGAGAG CGATGGAGCA ACTCAACCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGTTGC CCGAGGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTCGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC GGTGGAATGC
    TTGGTTTTGC TGTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGCTGTACA
    CCCCTTCATT TGGCAGCAAG AAATGGGCAG AAGAAATGTA TGAGTAAATT ATTAGAATAT
    AGCGCTGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAACAGTGCA GTGCTTGCTA
    GACAGTGGTG CTGATATTAA CAGGCCAAAT GTATCAGGAG CAACTCCATT GTACTTTGCT
    TGCAGTCATG GTCAGAGAGA TACAGCACAG ATCCTACTAT TACGAGGAGC CAAATATCTG
    CCAGATAAAA ATGGAGTAAC TCCTCTGGAT TTATGTGTAC AGGGTGGATA TGGAGAGACT
    TGTGAAGTAT TAATTCAATA TCACCCGAGG CTTTTTCAGA CTATTATTCA AATGACACAG
    AATGAAGACC TCCGAGAAAA CATGTTACGG CAAGTTCTGG AGCATTTGTC TCAGCAAAGT
    GAAAGCCAGT ACCTAAAGAT TCTAACAAGC CTTGCTGAAG TTGCTACAAC AAATGGTCAT
    AAACTGCTTA GCCTCTCTAG CAATTATGAT GCTCAAATGA AGAGCCTTTT AAGGATTGTG
    AGAATGTTTT GTCACGTCTT TCGAATTGGT CCATCCTCCC CCAGTAATGG AATTGATATG
    GGCTACAATG GGAATAAAAC TCCAAGAAGC CAGGTGTTCA AGCCTCTGGA ATTGCTTTGG
    CACTCGTTAG ATGAATGGCT AGTTTTAATA GCCACAGAAT TGATGAAAAA CAAAAGAGAC
    TCAACAGAGA TCACTTCTAT TTTACTGAAA CAAAAAGGCC AAGATCAAGA TGCTGCTTCC
    ATTCCTCCAT TTGAACCTCC AGGACCTGGG AGCTATGAAA ATCTGTCCAC TGGCACAAGG
    GAATCTAAAC CAGATGCTCT TGCAGGGAGA CAGGAAGCCA GTGCAGATTG TCAGGATGTT
    ATTTCTATGA CAGCTAACCG GCTAAGTGCT GTCATTCAAG CTTTTTACAT GTGCTGTTCT
    TGTCAGATGC CTCCGGGAAT GACTTCACCT CGTTTCATTG AATTTGTCTG CAAACATGAT
    GAAGTTTTAA AATGCTTTGT TAATAGAAAT CCCAAAATTA TATTTGACCA CTTTCACTTT
    CTCCTTGAAT GTCCTGAGTT GATGTCAAGA TTCATGCATA TCATAAAAGC ACAGCCTTTT
    AAAGATCGCT GTGAATGGTT CTATGAACAT TTGCATTCAG GACAGCCAGA TTCAGATATG
    GTGCACAGGC CAGTGAATGA AAATGATATC CTGCTGGTTC ACAGAGATTC TATTTTTAGG
    AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT TGTGCAAAGC TAAAGCAAGG GATTGCTGTA
    CGGTTCCATG GAGAAGAAGG CATGGGTCAA GGTGTTGTGC GTGAGTGGTT TGATATTCTG
    TCCAATGAGA TAGTCAATCC TGATTATGCA TTGTTTACCC AGTCAGCTGA TGGAACAACT
    TTTCAGCCTA ATAGCAACTC TTATGTAAAT CCTGATCACT TGAACTATTT TCGGTTTGCT
    GGGCAGATCT TGGGATTAGC GTTGAACCAC AGGCAGCTGG TCAATATTTA CTTCACACGA
    TCCTTCTACA AGCACATTCT TGGTATTCCT GTAAATTACC AAGATGTGGC ATCCATTGAT
    CCAGAATATG CGAAAAATTT GCAATGGATT TTAGATAATG ATATAAGTGA TCTGGGTCTA
    GAACTAACTT TTTCTGTTGA GACTGATGTG TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA
    CCTGGGGGTG GGAGTATTCT TGTGACACAA AATAATAAAG CGGAGTACGT CCAGCTTGTT
    ACTGAACTTC GAATGACAAG AGCCATTCAG CCTCAGATCA ATGCTTTTTT ACAGGGCTTT
    CATATGTTCA TTCCACCCTC CCTCATACAG CTTTTTGATG AATATGAATT GGAGCTACTG
    CTTTCTGGCA TGCCAGAAAT TGATGTGAGT GATTGGATAA AAAATACAGA ATACACAAGT
    GGCTATGAAA GAGAAGATCC AGTTATTCAG TGGTTCTGGG AAGTTGTAGA AGACATTACT
    CAAGAGGAGA GAGTTCTTCT CTTACAGTTT GTTACGGGCA GTTCCAGGGT CCCACATGGT
    GGGTTTGCTA ATATCATGGG TGGAAGTGGA TTGCAAAACT TTACAATCGC TGCTGTGCCA
    TATACTCCAA ATCTTTTACC AACTTCAAGC ACATGCATCA ACATGCTCAA GTTACCTGAA
    TACCCAAGTA AAGAAATACT CAAGGACAGA CTTCTTGTGG CACTACATTG TGGCAGCTAT
    GGTTACACAA TGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu40896
    Clone ID Related Accession (Same CDS sequence) NM_001350558.1 , NM_001350557.2 , XM_017011119.1 , XM_017011119.2 , NM_001350558.2 , NM_001321084.1 , NM_001321084.2 , XM_017011118.1 , NM_001350557.1
    Accession Version NM_001350558.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2226bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-22
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HACE1 isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357315.14, AL513472.9 and AL590402.10. On Apr 20, 2017 this sequence version replaced XM_017011116.1. Transcript Variant: This variant (10), as well as variants 5 and 9, encodes isoform d. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.191812.1, SRR1803612.263449.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
    ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT
    CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT
    GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA
    ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC
    CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC
    CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC
    CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT
    CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG
    AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT
    GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC
    ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT
    GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA
    GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA
    GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT
    CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA
    TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT
    CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT
    GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA
    GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA
    GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA
    GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA
    GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT
    AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG
    GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG
    CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG
    AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT
    TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG
    AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA
    ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT
    CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG
    CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA
    GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA
    GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT
    ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT
    CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA
    GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG
    GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337487.1
    CDS699..2924
    Translation

    Target ORF information:

    RefSeq Version NM_001350558.1
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001350558.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
    ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT
    CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT
    GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA
    ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC
    CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC
    CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC
    CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT
    CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG
    AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT
    GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC
    ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT
    GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA
    GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA
    GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT
    CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA
    TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT
    CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT
    GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA
    GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA
    GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA
    GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA
    GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT
    AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG
    GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG
    CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG
    AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT
    TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG
    AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA
    ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT
    CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG
    CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA
    GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA
    GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT
    ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT
    CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA
    GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG
    GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu40896
    Clone ID Related Accession (Same CDS sequence) NM_001350558.1 , NM_001350557.2 , XM_017011119.1 , XM_017011119.2 , NM_001350558.2 , NM_001321084.1 , NM_001321084.2 , XM_017011118.1 , NM_001350557.1
    Accession Version NM_001350557.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2226bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-04
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HACE1 isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357315.14, AL513472.9 and AL590402.10. On May 31, 2019 this sequence version replaced NM_001350557.1. Transcript Variant: This variant (9), as well as variants 5 and 10, encodes isoform d. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.1296.1, SRR1660807.101354.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
    ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT
    CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT
    GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA
    ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC
    CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC
    CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC
    CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT
    CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG
    AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT
    GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC
    ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT
    GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA
    GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA
    GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT
    CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA
    TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT
    CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT
    GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA
    GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA
    GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA
    GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA
    GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT
    AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG
    GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG
    CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG
    AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT
    TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG
    AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA
    ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT
    CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG
    CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA
    GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA
    GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT
    ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT
    CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA
    GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG
    GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337486.1
    CDS623..2848
    Translation

    Target ORF information:

    RefSeq Version NM_001350557.2
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001350557.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
    ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT
    CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT
    GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA
    ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC
    CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC
    CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC
    CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT
    CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG
    AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT
    GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC
    ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT
    GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA
    GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA
    GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT
    CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA
    TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT
    CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT
    GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA
    GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA
    GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA
    GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA
    GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT
    AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG
    GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG
    CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG
    AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT
    TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG
    AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA
    ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT
    CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG
    CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA
    GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA
    GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT
    ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT
    CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA
    GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG
    GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu40896
    Clone ID Related Accession (Same CDS sequence) NM_001350558.1 , NM_001350557.2 , XM_017011119.1 , XM_017011119.2 , NM_001350558.2 , NM_001321084.1 , NM_001321084.2 , XM_017011118.1 , NM_001350557.1
    Accession Version NM_001350558.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2226bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-04
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HACE1 isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357315.14, AL513472.9 and AL590402.10. On May 31, 2019 this sequence version replaced NM_001350558.1. Transcript Variant: This variant (10), as well as variants 5 and 9, encodes isoform d. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.191812.1, SRR1803612.263449.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
    ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT
    CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT
    GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA
    ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC
    CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC
    CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC
    CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT
    CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG
    AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT
    GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC
    ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT
    GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA
    GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA
    GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT
    CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA
    TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT
    CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT
    GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA
    GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA
    GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA
    GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA
    GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT
    AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG
    GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG
    CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG
    AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT
    TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG
    AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA
    ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT
    CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG
    CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA
    GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA
    GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT
    ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT
    CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA
    GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG
    GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337487.1
    CDS699..2924
    Translation

    Target ORF information:

    RefSeq Version NM_001350558.2
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001350558.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
    ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT
    CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT
    GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA
    ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC
    CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC
    CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC
    CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT
    CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG
    AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT
    GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC
    ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT
    GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA
    GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA
    GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT
    CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA
    TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT
    CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT
    GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA
    GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA
    GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA
    GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA
    GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT
    AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG
    GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG
    CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG
    AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT
    TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG
    AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA
    ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT
    CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG
    CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA
    GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA
    GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT
    ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT
    CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA
    GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG
    GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu40896
    Clone ID Related Accession (Same CDS sequence) NM_001350558.1 , NM_001350557.2 , XM_017011119.1 , XM_017011119.2 , NM_001350558.2 , NM_001321084.1 , NM_001321084.2 , XM_017011118.1 , NM_001350557.1
    Accession Version NM_001321084.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2226bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-22
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HACE1 isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB094058.1, BC034982.2, AL513472.9 and AL590402.10. On Mar 18, 2016 this sequence version replaced XM_006715530.2. Transcript Variant: This variant (5), as well as variants 9 and 10, encodes isoform d. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.167363.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
    ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT
    CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT
    GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA
    ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC
    CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC
    CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC
    CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT
    CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG
    AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT
    GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC
    ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT
    GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA
    GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA
    GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT
    CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA
    TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT
    CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT
    GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA
    GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA
    GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA
    GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA
    GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT
    AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG
    GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG
    CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG
    AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT
    TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG
    AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA
    ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT
    CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG
    CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA
    GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA
    GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT
    ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT
    CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA
    GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG
    GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001308013.1
    CDS514..2739
    Translation

    Target ORF information:

    RefSeq Version NM_001321084.1
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001321084.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
    ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT
    CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT
    GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA
    ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC
    CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC
    CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC
    CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT
    CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG
    AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT
    GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC
    ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT
    GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA
    GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA
    GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT
    CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA
    TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT
    CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT
    GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA
    GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA
    GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA
    GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA
    GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT
    AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG
    GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG
    CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG
    AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT
    TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG
    AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA
    ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT
    CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG
    CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA
    GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA
    GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT
    ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT
    CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA
    GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG
    GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu40896
    Clone ID Related Accession (Same CDS sequence) NM_001350558.1 , NM_001350557.2 , XM_017011119.1 , XM_017011119.2 , NM_001350558.2 , NM_001321084.1 , NM_001321084.2 , XM_017011118.1 , NM_001350557.1
    Accession Version NM_001321084.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2226bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-04
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HACE1 isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB094058.1, BC034982.2, AL513472.9 and AL590402.10. On May 31, 2019 this sequence version replaced NM_001321084.1. Transcript Variant: This variant (5), as well as variants 9 and 10, encodes isoform d. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.167363.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
    ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT
    CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT
    GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA
    ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC
    CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC
    CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC
    CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT
    CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG
    AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT
    GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC
    ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT
    GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA
    GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA
    GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT
    CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA
    TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT
    CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT
    GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA
    GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA
    GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA
    GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA
    GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT
    AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG
    GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG
    CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG
    AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT
    TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG
    AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA
    ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT
    CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG
    CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA
    GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA
    GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT
    ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT
    CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA
    GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG
    GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001308013.1
    CDS514..2739
    Translation

    Target ORF information:

    RefSeq Version NM_001321084.2
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001321084.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
    ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT
    CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT
    GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA
    ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC
    CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC
    CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC
    CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT
    CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG
    AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT
    GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC
    ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT
    GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA
    GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA
    GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT
    CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA
    TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT
    CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT
    GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA
    GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA
    GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA
    GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA
    GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT
    AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG
    GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG
    CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG
    AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT
    TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG
    AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA
    ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT
    CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG
    CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA
    GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA
    GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT
    ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT
    CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA
    GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG
    GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu40896
    Clone ID Related Accession (Same CDS sequence) NM_001350558.1 , NM_001350557.2 , XM_017011119.1 , XM_017011119.2 , NM_001350558.2 , NM_001321084.1 , NM_001321084.2 , XM_017011118.1 , NM_001350557.1
    Accession Version NM_001350557.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2226bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-22
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HACE1 isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357315.14, AL513472.9 and AL590402.10. On Apr 20, 2017 this sequence version replaced XM_017011120.1. Transcript Variant: This variant (9), as well as variants 5 and 10, encodes isoform d. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.1296.1, SRR1660807.101354.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
    ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT
    CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT
    GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA
    ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC
    CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC
    CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC
    CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT
    CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG
    AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT
    GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC
    ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT
    GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA
    GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA
    GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT
    CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA
    TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT
    CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT
    GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA
    GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA
    GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA
    GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA
    GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT
    AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG
    GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG
    CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG
    AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT
    TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG
    AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA
    ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT
    CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG
    CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA
    GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA
    GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT
    ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT
    CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA
    GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG
    GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337486.1
    CDS623..2848
    Translation

    Target ORF information:

    RefSeq Version NM_001350557.1
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001350557.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
    ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT
    CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT
    GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA
    ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC
    CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC
    CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC
    CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT
    CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG
    AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT
    GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC
    ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT
    GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA
    GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA
    GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT
    CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA
    TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT
    CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT
    GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA
    GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA
    GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA
    GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA
    GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT
    AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG
    GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG
    CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG
    AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT
    TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG
    AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA
    ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT
    CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG
    CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA
    GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA
    GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT
    ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT
    CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA
    GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG
    GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu70565
    Clone ID Related Accession (Same CDS sequence) NM_001350556.2 , NM_001350556.1
    Accession Version NM_001350556.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2244bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-04
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HACE1 isoform g
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357315.14, AL513472.9 and AL590402.10. On May 31, 2019 this sequence version replaced NM_001350556.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.112650.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGGCAGA CAGCACTGCA TGTTGCCTGC CAGAACGGTC ACAAGACGAC AGTGCAGTGC 
    TTGCTAGACA GTGGTGCTGA TATTAACAGG CCAAATGTAT CAGGAGCAAC TCCATTGTAC
    TTTGCTTGCA GTCATGGTCA GAGAGATACA GCACAGATCC TACTATTACG AGGAGCCAAA
    TATCTGCCAG ATAAAAATGG AGTAACTCCT CTGGATTTAT GTGTACAGGG TGGATATGGA
    GAGACTTGTG AAGTATTAAT TCAATATCAC CCGAGGCTTT TTCAGACTAT TATTCAAATG
    ACACAGAATG AAGACCTCCG AGAAAACATG TTACGGCAAG TTCTGGAGCA TTTGTCTCAG
    CAAAGTGAAA GCCAGTACCT AAAGATTCTA ACAAGCCTTG CTGAAGTTGC TACAACAAAT
    GGTCATAAAC TGCTTAGCCT CTCTAGCAAT TATGATGCTC AAATGAAGAG CCTTTTAAGG
    ATTGTGAGAA TGTTTTGTCA CGTCTTTCGA ATTGGTCCAT CCTCCCCCAG TAATGGAATT
    GATATGGGCT ACAATGGGAA TAAAACTCCA AGAAGCCAGG TGTTCAAGCC TCTGGAATTG
    CTTTGGCACT CGTTAGATGA ATGGCTAGTT TTAATAGCCA CAGAATTGAT GAAAAACAAA
    AGAGACTCAA CAGAGATCAC TTCTATTTTA CTGAAACAAA AAGGCCAAGA TCAAGATGCT
    GCTTCCATTC CTCCATTTGA ACCTCCAGGA CCTGGGAGCT ATGAAAATCT GTCCACTGGC
    ACAAGGGAAT CTAAACCAGA TGCTCTTGCA GGGAGACAGG AAGCCAGTGC AGATTGTCAG
    GATGTTATTT CTATGACAGC TAACCGGCTA AGTGCTGTCA TTCAAGCTTT TTACATGTGC
    TGTTCTTGTC AGATGCCTCC GGGAATGACT TCACCTCGTT TCATTGAATT TGTCTGCAAA
    CATGATGAAG TTTTAAAATG CTTTGTTAAT AGAAATCCCA AAATTATATT TGACCACTTT
    CACTTTCTCC TTGAATGTCC TGAGTTGATG TCAAGATTCA TGCATATCAT AAAAGCACAG
    CCTTTTAAAG ATCGCTGTGA ATGGTTCTAT GAACATTTGC ATTCAGGACA GCCAGATTCA
    GATATGGTGC ACAGGCCAGT GAATGAAAAT GATATCCTGC TGGTTCACAG AGATTCTATT
    TTTAGGAGTA GCTGTGAAGT TGTGTCAAAA GCAAATTGTG CAAAGCTAAA GCAAGGGATT
    GCTGTACGGT TCCATGGAGA AGAAGGCATG GGTCAAGGTG TTGTGCGTGA GTGGTTTGAT
    ATTCTGTCCA ATGAGATAGT CAATCCTGAT TATGCATTGT TTACCCAGTC AGCTGATGGA
    ACAACTTTTC AGCCTAATAG CAACTCTTAT GTAAATCCTG ATCACTTGAA CTATTTTCGG
    TTTGCTGGGC AGATCTTGGG ATTAGCGTTG AACCACAGGC AGCTGGTCAA TATTTACTTC
    ACACGATCCT TCTACAAGCA CATTCTTGGT ATTCCTGTAA ATTACCAAGA TGTGGCATCC
    ATTGATCCAG AATATGCGAA AAATTTGCAA TGGATTTTAG ATAATGATAT AAGTGATCTG
    GGTCTAGAAC TAACTTTTTC TGTTGAGACT GATGTGTTTG GAGCAATGGA AGAGGTGCCT
    TTGAAACCTG GGGGTGGGAG TATTCTTGTG ACACAAAATA ATAAAGCGGA GTACGTCCAG
    CTTGTTACTG AACTTCGAAT GACAAGAGCC ATTCAGCCTC AGATCAATGC TTTTTTACAG
    GGCTTTCATA TGTTCATTCC ACCCTCCCTC ATACAGCTTT TTGATGAATA TGAATTGGAG
    CTACTGCTTT CTGGCATGCC AGAAATTGAT GTGAGTGATT GGATAAAAAA TACAGAATAC
    ACAAGTGGCT ATGAAAGAGA AGATCCAGTT ATTCAGTGGT TCTGGGAAGT TGTAGAAGAC
    ATTACTCAAG AGGAGAGAGT TCTTCTCTTA CAGTTTGTTA CGGGCAGTTC CAGGGTCCCA
    CATGGTGGGT TTGCTAATAT CATGGGTGGA AGTGGATTGC AAAACTTTAC AATCGCTGCT
    GTGCCATATA CTCCAAATCT TTTACCAACT TCAAGCACAT GCATCAACAT GCTCAAGTTA
    CCTGAATACC CAAGTAAAGA AATACTCAAG GACAGACTTC TTGTGGCACT ACATTGTGGC
    AGCTATGGTT ACACAATGGC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337485.1
    CDS688..2931
    Translation

    Target ORF information:

    RefSeq Version NM_001350556.2
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001350556.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGGCAGA CAGCACTGCA TGTTGCCTGC CAGAACGGTC ACAAGACGAC AGTGCAGTGC 
    TTGCTAGACA GTGGTGCTGA TATTAACAGG CCAAATGTAT CAGGAGCAAC TCCATTGTAC
    TTTGCTTGCA GTCATGGTCA GAGAGATACA GCACAGATCC TACTATTACG AGGAGCCAAA
    TATCTGCCAG ATAAAAATGG AGTAACTCCT CTGGATTTAT GTGTACAGGG TGGATATGGA
    GAGACTTGTG AAGTATTAAT TCAATATCAC CCGAGGCTTT TTCAGACTAT TATTCAAATG
    ACACAGAATG AAGACCTCCG AGAAAACATG TTACGGCAAG TTCTGGAGCA TTTGTCTCAG
    CAAAGTGAAA GCCAGTACCT AAAGATTCTA ACAAGCCTTG CTGAAGTTGC TACAACAAAT
    GGTCATAAAC TGCTTAGCCT CTCTAGCAAT TATGATGCTC AAATGAAGAG CCTTTTAAGG
    ATTGTGAGAA TGTTTTGTCA CGTCTTTCGA ATTGGTCCAT CCTCCCCCAG TAATGGAATT
    GATATGGGCT ACAATGGGAA TAAAACTCCA AGAAGCCAGG TGTTCAAGCC TCTGGAATTG
    CTTTGGCACT CGTTAGATGA ATGGCTAGTT TTAATAGCCA CAGAATTGAT GAAAAACAAA
    AGAGACTCAA CAGAGATCAC TTCTATTTTA CTGAAACAAA AAGGCCAAGA TCAAGATGCT
    GCTTCCATTC CTCCATTTGA ACCTCCAGGA CCTGGGAGCT ATGAAAATCT GTCCACTGGC
    ACAAGGGAAT CTAAACCAGA TGCTCTTGCA GGGAGACAGG AAGCCAGTGC AGATTGTCAG
    GATGTTATTT CTATGACAGC TAACCGGCTA AGTGCTGTCA TTCAAGCTTT TTACATGTGC
    TGTTCTTGTC AGATGCCTCC GGGAATGACT TCACCTCGTT TCATTGAATT TGTCTGCAAA
    CATGATGAAG TTTTAAAATG CTTTGTTAAT AGAAATCCCA AAATTATATT TGACCACTTT
    CACTTTCTCC TTGAATGTCC TGAGTTGATG TCAAGATTCA TGCATATCAT AAAAGCACAG
    CCTTTTAAAG ATCGCTGTGA ATGGTTCTAT GAACATTTGC ATTCAGGACA GCCAGATTCA
    GATATGGTGC ACAGGCCAGT GAATGAAAAT GATATCCTGC TGGTTCACAG AGATTCTATT
    TTTAGGAGTA GCTGTGAAGT TGTGTCAAAA GCAAATTGTG CAAAGCTAAA GCAAGGGATT
    GCTGTACGGT TCCATGGAGA AGAAGGCATG GGTCAAGGTG TTGTGCGTGA GTGGTTTGAT
    ATTCTGTCCA ATGAGATAGT CAATCCTGAT TATGCATTGT TTACCCAGTC AGCTGATGGA
    ACAACTTTTC AGCCTAATAG CAACTCTTAT GTAAATCCTG ATCACTTGAA CTATTTTCGG
    TTTGCTGGGC AGATCTTGGG ATTAGCGTTG AACCACAGGC AGCTGGTCAA TATTTACTTC
    ACACGATCCT TCTACAAGCA CATTCTTGGT ATTCCTGTAA ATTACCAAGA TGTGGCATCC
    ATTGATCCAG AATATGCGAA AAATTTGCAA TGGATTTTAG ATAATGATAT AAGTGATCTG
    GGTCTAGAAC TAACTTTTTC TGTTGAGACT GATGTGTTTG GAGCAATGGA AGAGGTGCCT
    TTGAAACCTG GGGGTGGGAG TATTCTTGTG ACACAAAATA ATAAAGCGGA GTACGTCCAG
    CTTGTTACTG AACTTCGAAT GACAAGAGCC ATTCAGCCTC AGATCAATGC TTTTTTACAG
    GGCTTTCATA TGTTCATTCC ACCCTCCCTC ATACAGCTTT TTGATGAATA TGAATTGGAG
    CTACTGCTTT CTGGCATGCC AGAAATTGAT GTGAGTGATT GGATAAAAAA TACAGAATAC
    ACAAGTGGCT ATGAAAGAGA AGATCCAGTT ATTCAGTGGT TCTGGGAAGT TGTAGAAGAC
    ATTACTCAAG AGGAGAGAGT TCTTCTCTTA CAGTTTGTTA CGGGCAGTTC CAGGGTCCCA
    CATGGTGGGT TTGCTAATAT CATGGGTGGA AGTGGATTGC AAAACTTTAC AATCGCTGCT
    GTGCCATATA CTCCAAATCT TTTACCAACT TCAAGCACAT GCATCAACAT GCTCAAGTTA
    CCTGAATACC CAAGTAAAGA AATACTCAAG GACAGACTTC TTGTGGCACT ACATTGTGGC
    AGCTATGGTT ACACAATGGC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu70565
    Clone ID Related Accession (Same CDS sequence) NM_001350556.2 , NM_001350556.1
    Accession Version NM_001350556.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2244bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-22
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HACE1 isoform g
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357315.14, AL513472.9 and AL590402.10. On Apr 20, 2017 this sequence version replaced XM_017011115.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.112650.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGGCAGA CAGCACTGCA TGTTGCCTGC CAGAACGGTC ACAAGACGAC AGTGCAGTGC 
    TTGCTAGACA GTGGTGCTGA TATTAACAGG CCAAATGTAT CAGGAGCAAC TCCATTGTAC
    TTTGCTTGCA GTCATGGTCA GAGAGATACA GCACAGATCC TACTATTACG AGGAGCCAAA
    TATCTGCCAG ATAAAAATGG AGTAACTCCT CTGGATTTAT GTGTACAGGG TGGATATGGA
    GAGACTTGTG AAGTATTAAT TCAATATCAC CCGAGGCTTT TTCAGACTAT TATTCAAATG
    ACACAGAATG AAGACCTCCG AGAAAACATG TTACGGCAAG TTCTGGAGCA TTTGTCTCAG
    CAAAGTGAAA GCCAGTACCT AAAGATTCTA ACAAGCCTTG CTGAAGTTGC TACAACAAAT
    GGTCATAAAC TGCTTAGCCT CTCTAGCAAT TATGATGCTC AAATGAAGAG CCTTTTAAGG
    ATTGTGAGAA TGTTTTGTCA CGTCTTTCGA ATTGGTCCAT CCTCCCCCAG TAATGGAATT
    GATATGGGCT ACAATGGGAA TAAAACTCCA AGAAGCCAGG TGTTCAAGCC TCTGGAATTG
    CTTTGGCACT CGTTAGATGA ATGGCTAGTT TTAATAGCCA CAGAATTGAT GAAAAACAAA
    AGAGACTCAA CAGAGATCAC TTCTATTTTA CTGAAACAAA AAGGCCAAGA TCAAGATGCT
    GCTTCCATTC CTCCATTTGA ACCTCCAGGA CCTGGGAGCT ATGAAAATCT GTCCACTGGC
    ACAAGGGAAT CTAAACCAGA TGCTCTTGCA GGGAGACAGG AAGCCAGTGC AGATTGTCAG
    GATGTTATTT CTATGACAGC TAACCGGCTA AGTGCTGTCA TTCAAGCTTT TTACATGTGC
    TGTTCTTGTC AGATGCCTCC GGGAATGACT TCACCTCGTT TCATTGAATT TGTCTGCAAA
    CATGATGAAG TTTTAAAATG CTTTGTTAAT AGAAATCCCA AAATTATATT TGACCACTTT
    CACTTTCTCC TTGAATGTCC TGAGTTGATG TCAAGATTCA TGCATATCAT AAAAGCACAG
    CCTTTTAAAG ATCGCTGTGA ATGGTTCTAT GAACATTTGC ATTCAGGACA GCCAGATTCA
    GATATGGTGC ACAGGCCAGT GAATGAAAAT GATATCCTGC TGGTTCACAG AGATTCTATT
    TTTAGGAGTA GCTGTGAAGT TGTGTCAAAA GCAAATTGTG CAAAGCTAAA GCAAGGGATT
    GCTGTACGGT TCCATGGAGA AGAAGGCATG GGTCAAGGTG TTGTGCGTGA GTGGTTTGAT
    ATTCTGTCCA ATGAGATAGT CAATCCTGAT TATGCATTGT TTACCCAGTC AGCTGATGGA
    ACAACTTTTC AGCCTAATAG CAACTCTTAT GTAAATCCTG ATCACTTGAA CTATTTTCGG
    TTTGCTGGGC AGATCTTGGG ATTAGCGTTG AACCACAGGC AGCTGGTCAA TATTTACTTC
    ACACGATCCT TCTACAAGCA CATTCTTGGT ATTCCTGTAA ATTACCAAGA TGTGGCATCC
    ATTGATCCAG AATATGCGAA AAATTTGCAA TGGATTTTAG ATAATGATAT AAGTGATCTG
    GGTCTAGAAC TAACTTTTTC TGTTGAGACT GATGTGTTTG GAGCAATGGA AGAGGTGCCT
    TTGAAACCTG GGGGTGGGAG TATTCTTGTG ACACAAAATA ATAAAGCGGA GTACGTCCAG
    CTTGTTACTG AACTTCGAAT GACAAGAGCC ATTCAGCCTC AGATCAATGC TTTTTTACAG
    GGCTTTCATA TGTTCATTCC ACCCTCCCTC ATACAGCTTT TTGATGAATA TGAATTGGAG
    CTACTGCTTT CTGGCATGCC AGAAATTGAT GTGAGTGATT GGATAAAAAA TACAGAATAC
    ACAAGTGGCT ATGAAAGAGA AGATCCAGTT ATTCAGTGGT TCTGGGAAGT TGTAGAAGAC
    ATTACTCAAG AGGAGAGAGT TCTTCTCTTA CAGTTTGTTA CGGGCAGTTC CAGGGTCCCA
    CATGGTGGGT TTGCTAATAT CATGGGTGGA AGTGGATTGC AAAACTTTAC AATCGCTGCT
    GTGCCATATA CTCCAAATCT TTTACCAACT TCAAGCACAT GCATCAACAT GCTCAAGTTA
    CCTGAATACC CAAGTAAAGA AATACTCAAG GACAGACTTC TTGTGGCACT ACATTGTGGC
    AGCTATGGTT ACACAATGGC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337485.1
    CDS688..2931
    Translation

    Target ORF information:

    RefSeq Version NM_001350556.1
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001350556.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGGCAGA CAGCACTGCA TGTTGCCTGC CAGAACGGTC ACAAGACGAC AGTGCAGTGC 
    TTGCTAGACA GTGGTGCTGA TATTAACAGG CCAAATGTAT CAGGAGCAAC TCCATTGTAC
    TTTGCTTGCA GTCATGGTCA GAGAGATACA GCACAGATCC TACTATTACG AGGAGCCAAA
    TATCTGCCAG ATAAAAATGG AGTAACTCCT CTGGATTTAT GTGTACAGGG TGGATATGGA
    GAGACTTGTG AAGTATTAAT TCAATATCAC CCGAGGCTTT TTCAGACTAT TATTCAAATG
    ACACAGAATG AAGACCTCCG AGAAAACATG TTACGGCAAG TTCTGGAGCA TTTGTCTCAG
    CAAAGTGAAA GCCAGTACCT AAAGATTCTA ACAAGCCTTG CTGAAGTTGC TACAACAAAT
    GGTCATAAAC TGCTTAGCCT CTCTAGCAAT TATGATGCTC AAATGAAGAG CCTTTTAAGG
    ATTGTGAGAA TGTTTTGTCA CGTCTTTCGA ATTGGTCCAT CCTCCCCCAG TAATGGAATT
    GATATGGGCT ACAATGGGAA TAAAACTCCA AGAAGCCAGG TGTTCAAGCC TCTGGAATTG
    CTTTGGCACT CGTTAGATGA ATGGCTAGTT TTAATAGCCA CAGAATTGAT GAAAAACAAA
    AGAGACTCAA CAGAGATCAC TTCTATTTTA CTGAAACAAA AAGGCCAAGA TCAAGATGCT
    GCTTCCATTC CTCCATTTGA ACCTCCAGGA CCTGGGAGCT ATGAAAATCT GTCCACTGGC
    ACAAGGGAAT CTAAACCAGA TGCTCTTGCA GGGAGACAGG AAGCCAGTGC AGATTGTCAG
    GATGTTATTT CTATGACAGC TAACCGGCTA AGTGCTGTCA TTCAAGCTTT TTACATGTGC
    TGTTCTTGTC AGATGCCTCC GGGAATGACT TCACCTCGTT TCATTGAATT TGTCTGCAAA
    CATGATGAAG TTTTAAAATG CTTTGTTAAT AGAAATCCCA AAATTATATT TGACCACTTT
    CACTTTCTCC TTGAATGTCC TGAGTTGATG TCAAGATTCA TGCATATCAT AAAAGCACAG
    CCTTTTAAAG ATCGCTGTGA ATGGTTCTAT GAACATTTGC ATTCAGGACA GCCAGATTCA
    GATATGGTGC ACAGGCCAGT GAATGAAAAT GATATCCTGC TGGTTCACAG AGATTCTATT
    TTTAGGAGTA GCTGTGAAGT TGTGTCAAAA GCAAATTGTG CAAAGCTAAA GCAAGGGATT
    GCTGTACGGT TCCATGGAGA AGAAGGCATG GGTCAAGGTG TTGTGCGTGA GTGGTTTGAT
    ATTCTGTCCA ATGAGATAGT CAATCCTGAT TATGCATTGT TTACCCAGTC AGCTGATGGA
    ACAACTTTTC AGCCTAATAG CAACTCTTAT GTAAATCCTG ATCACTTGAA CTATTTTCGG
    TTTGCTGGGC AGATCTTGGG ATTAGCGTTG AACCACAGGC AGCTGGTCAA TATTTACTTC
    ACACGATCCT TCTACAAGCA CATTCTTGGT ATTCCTGTAA ATTACCAAGA TGTGGCATCC
    ATTGATCCAG AATATGCGAA AAATTTGCAA TGGATTTTAG ATAATGATAT AAGTGATCTG
    GGTCTAGAAC TAACTTTTTC TGTTGAGACT GATGTGTTTG GAGCAATGGA AGAGGTGCCT
    TTGAAACCTG GGGGTGGGAG TATTCTTGTG ACACAAAATA ATAAAGCGGA GTACGTCCAG
    CTTGTTACTG AACTTCGAAT GACAAGAGCC ATTCAGCCTC AGATCAATGC TTTTTTACAG
    GGCTTTCATA TGTTCATTCC ACCCTCCCTC ATACAGCTTT TTGATGAATA TGAATTGGAG
    CTACTGCTTT CTGGCATGCC AGAAATTGAT GTGAGTGATT GGATAAAAAA TACAGAATAC
    ACAAGTGGCT ATGAAAGAGA AGATCCAGTT ATTCAGTGGT TCTGGGAAGT TGTAGAAGAC
    ATTACTCAAG AGGAGAGAGT TCTTCTCTTA CAGTTTGTTA CGGGCAGTTC CAGGGTCCCA
    CATGGTGGGT TTGCTAATAT CATGGGTGGA AGTGGATTGC AAAACTTTAC AATCGCTGCT
    GTGCCATATA CTCCAAATCT TTTACCAACT TCAAGCACAT GCATCAACAT GCTCAAGTTA
    CCTGAATACC CAAGTAAAGA AATACTCAAG GACAGACTTC TTGTGGCACT ACATTGTGGC
    AGCTATGGTT ACACAATGGC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu70566
    Clone ID Related Accession (Same CDS sequence) NM_001350559.1 , NM_001350559.2 , XM_017011122.1
    Accession Version NM_001350559.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2148bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-22
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HACE1 isoform h
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357315.14, AL513472.9 and AL590402.10. On Apr 20, 2017 this sequence version replaced XM_017011121.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.97975.1, SRR1803617.143672.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTCAACAT TTGTAATAAT GAAGGCCTTA CAGCATCATG GTCAGAGAGA TACAGCACAG 
    ATCCTACTAT TACGAGGAGC CAAATATCTG CCAGATAAAA ATGGAGTAAC TCCTCTGGAT
    TTATGTGTAC AGGGTGGATA TGGAGAGACT TGTGAAGTAT TAATTCAATA TCACCCGAGG
    CTTTTTCAGA CTATTATTCA AATGACACAG AATGAAGACC TCCGAGAAAA CATGTTACGG
    CAAGTTCTGG AGCATTTGTC TCAGCAAAGT GAAAGCCAGT ACCTAAAGAT TCTAACAAGC
    CTTGCTGAAG TTGCTACAAC AAATGGTCAT AAACTGCTTA GCCTCTCTAG CAATTATGAT
    GCTCAAATGA AGAGCCTTTT AAGGATTGTG AGAATGTTTT GTCACGTCTT TCGAATTGGT
    CCATCCTCCC CCAGTAATGG AATTGATATG GGCTACAATG GGAATAAAAC TCCAAGAAGC
    CAGGTGTTCA AGCCTCTGGA ATTGCTTTGG CACTCGTTAG ATGAATGGCT AGTTTTAATA
    GCCACAGAAT TGATGAAAAA CAAAAGAGAC TCAACAGAGA TCACTTCTAT TTTACTGAAA
    CAAAAAGGCC AAGATCAAGA TGCTGCTTCC ATTCCTCCAT TTGAACCTCC AGGACCTGGG
    AGCTATGAAA ATCTGTCCAC TGGCACAAGG GAATCTAAAC CAGATGCTCT TGCAGGGAGA
    CAGGAAGCCA GTGCAGATTG TCAGGATGTT ATTTCTATGA CAGCTAACCG GCTAAGTGCT
    GTCATTCAAG CTTTTTACAT GTGCTGTTCT TGTCAGATGC CTCCGGGAAT GACTTCACCT
    CGTTTCATTG AATTTGTCTG CAAACATGAT GAAGTTTTAA AATGCTTTGT TAATAGAAAT
    CCCAAAATTA TATTTGACCA CTTTCACTTT CTCCTTGAAT GTCCTGAGTT GATGTCAAGA
    TTCATGCATA TCATAAAAGC ACAGCCTTTT AAAGATCGCT GTGAATGGTT CTATGAACAT
    TTGCATTCAG GACAGCCAGA TTCAGATATG GTGCACAGGC CAGTGAATGA AAATGATATC
    CTGCTGGTTC ACAGAGATTC TATTTTTAGG AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT
    TGTGCAAAGC TAAAGCAAGG GATTGCTGTA CGGTTCCATG GAGAAGAAGG CATGGGTCAA
    GGTGTTGTGC GTGAGTGGTT TGATATTCTG TCCAATGAGA TAGTCAATCC TGATTATGCA
    TTGTTTACCC AGTCAGCTGA TGGAACAACT TTTCAGCCTA ATAGCAACTC TTATGTAAAT
    CCTGATCACT TGAACTATTT TCGGTTTGCT GGGCAGATCT TGGGATTAGC GTTGAACCAC
    AGGCAGCTGG TCAATATTTA CTTCACACGA TCCTTCTACA AGCACATTCT TGGTATTCCT
    GTAAATTACC AAGATGTGGC ATCCATTGAT CCAGAATATG CGAAAAATTT GCAATGGATT
    TTAGATAATG ATATAAGTGA TCTGGGTCTA GAACTAACTT TTTCTGTTGA GACTGATGTG
    TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA CCTGGGGGTG GGAGTATTCT TGTGACACAA
    AATAATAAAG CGGAGTACGT CCAGCTTGTT ACTGAACTTC GAATGACAAG AGCCATTCAG
    CCTCAGATCA ATGCTTTTTT ACAGGGCTTT CATATGTTCA TTCCACCCTC CCTCATACAG
    CTTTTTGATG AATATGAATT GGAGCTACTG CTTTCTGGCA TGCCAGAAAT TGATGTGAGT
    GATTGGATAA AAAATACAGA ATACACAAGT GGCTATGAAA GAGAAGATCC AGTTATTCAG
    TGGTTCTGGG AAGTTGTAGA AGACATTACT CAAGAGGAGA GAGTTCTTCT CTTACAGTTT
    GTTACGGGCA GTTCCAGGGT CCCACATGGT GGGTTTGCTA ATATCATGGG TGGAAGTGGA
    TTGCAAAACT TTACAATCGC TGCTGTGCCA TATACTCCAA ATCTTTTACC AACTTCAAGC
    ACATGCATCA ACATGCTCAA GTTACCTGAA TACCCAAGTA AAGAAATACT CAAGGACAGA
    CTTCTTGTGG CACTACATTG TGGCAGCTAT GGTTACACAA TGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337488.1
    CDS645..2792
    Translation

    Target ORF information:

    RefSeq Version NM_001350559.1
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001350559.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTCAACAT TTGTAATAAT GAAGGCCTTA CAGCATCATG GTCAGAGAGA TACAGCACAG 
    ATCCTACTAT TACGAGGAGC CAAATATCTG CCAGATAAAA ATGGAGTAAC TCCTCTGGAT
    TTATGTGTAC AGGGTGGATA TGGAGAGACT TGTGAAGTAT TAATTCAATA TCACCCGAGG
    CTTTTTCAGA CTATTATTCA AATGACACAG AATGAAGACC TCCGAGAAAA CATGTTACGG
    CAAGTTCTGG AGCATTTGTC TCAGCAAAGT GAAAGCCAGT ACCTAAAGAT TCTAACAAGC
    CTTGCTGAAG TTGCTACAAC AAATGGTCAT AAACTGCTTA GCCTCTCTAG CAATTATGAT
    GCTCAAATGA AGAGCCTTTT AAGGATTGTG AGAATGTTTT GTCACGTCTT TCGAATTGGT
    CCATCCTCCC CCAGTAATGG AATTGATATG GGCTACAATG GGAATAAAAC TCCAAGAAGC
    CAGGTGTTCA AGCCTCTGGA ATTGCTTTGG CACTCGTTAG ATGAATGGCT AGTTTTAATA
    GCCACAGAAT TGATGAAAAA CAAAAGAGAC TCAACAGAGA TCACTTCTAT TTTACTGAAA
    CAAAAAGGCC AAGATCAAGA TGCTGCTTCC ATTCCTCCAT TTGAACCTCC AGGACCTGGG
    AGCTATGAAA ATCTGTCCAC TGGCACAAGG GAATCTAAAC CAGATGCTCT TGCAGGGAGA
    CAGGAAGCCA GTGCAGATTG TCAGGATGTT ATTTCTATGA CAGCTAACCG GCTAAGTGCT
    GTCATTCAAG CTTTTTACAT GTGCTGTTCT TGTCAGATGC CTCCGGGAAT GACTTCACCT
    CGTTTCATTG AATTTGTCTG CAAACATGAT GAAGTTTTAA AATGCTTTGT TAATAGAAAT
    CCCAAAATTA TATTTGACCA CTTTCACTTT CTCCTTGAAT GTCCTGAGTT GATGTCAAGA
    TTCATGCATA TCATAAAAGC ACAGCCTTTT AAAGATCGCT GTGAATGGTT CTATGAACAT
    TTGCATTCAG GACAGCCAGA TTCAGATATG GTGCACAGGC CAGTGAATGA AAATGATATC
    CTGCTGGTTC ACAGAGATTC TATTTTTAGG AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT
    TGTGCAAAGC TAAAGCAAGG GATTGCTGTA CGGTTCCATG GAGAAGAAGG CATGGGTCAA
    GGTGTTGTGC GTGAGTGGTT TGATATTCTG TCCAATGAGA TAGTCAATCC TGATTATGCA
    TTGTTTACCC AGTCAGCTGA TGGAACAACT TTTCAGCCTA ATAGCAACTC TTATGTAAAT
    CCTGATCACT TGAACTATTT TCGGTTTGCT GGGCAGATCT TGGGATTAGC GTTGAACCAC
    AGGCAGCTGG TCAATATTTA CTTCACACGA TCCTTCTACA AGCACATTCT TGGTATTCCT
    GTAAATTACC AAGATGTGGC ATCCATTGAT CCAGAATATG CGAAAAATTT GCAATGGATT
    TTAGATAATG ATATAAGTGA TCTGGGTCTA GAACTAACTT TTTCTGTTGA GACTGATGTG
    TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA CCTGGGGGTG GGAGTATTCT TGTGACACAA
    AATAATAAAG CGGAGTACGT CCAGCTTGTT ACTGAACTTC GAATGACAAG AGCCATTCAG
    CCTCAGATCA ATGCTTTTTT ACAGGGCTTT CATATGTTCA TTCCACCCTC CCTCATACAG
    CTTTTTGATG AATATGAATT GGAGCTACTG CTTTCTGGCA TGCCAGAAAT TGATGTGAGT
    GATTGGATAA AAAATACAGA ATACACAAGT GGCTATGAAA GAGAAGATCC AGTTATTCAG
    TGGTTCTGGG AAGTTGTAGA AGACATTACT CAAGAGGAGA GAGTTCTTCT CTTACAGTTT
    GTTACGGGCA GTTCCAGGGT CCCACATGGT GGGTTTGCTA ATATCATGGG TGGAAGTGGA
    TTGCAAAACT TTACAATCGC TGCTGTGCCA TATACTCCAA ATCTTTTACC AACTTCAAGC
    ACATGCATCA ACATGCTCAA GTTACCTGAA TACCCAAGTA AAGAAATACT CAAGGACAGA
    CTTCTTGTGG CACTACATTG TGGCAGCTAT GGTTACACAA TGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu70566
    Clone ID Related Accession (Same CDS sequence) NM_001350559.1 , NM_001350559.2 , XM_017011122.1
    Accession Version NM_001350559.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2148bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-04
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HACE1 isoform h
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357315.14, AL513472.9 and AL590402.10. On May 31, 2019 this sequence version replaced NM_001350559.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.97975.1, SRR1803617.143672.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTCAACAT TTGTAATAAT GAAGGCCTTA CAGCATCATG GTCAGAGAGA TACAGCACAG 
    ATCCTACTAT TACGAGGAGC CAAATATCTG CCAGATAAAA ATGGAGTAAC TCCTCTGGAT
    TTATGTGTAC AGGGTGGATA TGGAGAGACT TGTGAAGTAT TAATTCAATA TCACCCGAGG
    CTTTTTCAGA CTATTATTCA AATGACACAG AATGAAGACC TCCGAGAAAA CATGTTACGG
    CAAGTTCTGG AGCATTTGTC TCAGCAAAGT GAAAGCCAGT ACCTAAAGAT TCTAACAAGC
    CTTGCTGAAG TTGCTACAAC AAATGGTCAT AAACTGCTTA GCCTCTCTAG CAATTATGAT
    GCTCAAATGA AGAGCCTTTT AAGGATTGTG AGAATGTTTT GTCACGTCTT TCGAATTGGT
    CCATCCTCCC CCAGTAATGG AATTGATATG GGCTACAATG GGAATAAAAC TCCAAGAAGC
    CAGGTGTTCA AGCCTCTGGA ATTGCTTTGG CACTCGTTAG ATGAATGGCT AGTTTTAATA
    GCCACAGAAT TGATGAAAAA CAAAAGAGAC TCAACAGAGA TCACTTCTAT TTTACTGAAA
    CAAAAAGGCC AAGATCAAGA TGCTGCTTCC ATTCCTCCAT TTGAACCTCC AGGACCTGGG
    AGCTATGAAA ATCTGTCCAC TGGCACAAGG GAATCTAAAC CAGATGCTCT TGCAGGGAGA
    CAGGAAGCCA GTGCAGATTG TCAGGATGTT ATTTCTATGA CAGCTAACCG GCTAAGTGCT
    GTCATTCAAG CTTTTTACAT GTGCTGTTCT TGTCAGATGC CTCCGGGAAT GACTTCACCT
    CGTTTCATTG AATTTGTCTG CAAACATGAT GAAGTTTTAA AATGCTTTGT TAATAGAAAT
    CCCAAAATTA TATTTGACCA CTTTCACTTT CTCCTTGAAT GTCCTGAGTT GATGTCAAGA
    TTCATGCATA TCATAAAAGC ACAGCCTTTT AAAGATCGCT GTGAATGGTT CTATGAACAT
    TTGCATTCAG GACAGCCAGA TTCAGATATG GTGCACAGGC CAGTGAATGA AAATGATATC
    CTGCTGGTTC ACAGAGATTC TATTTTTAGG AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT
    TGTGCAAAGC TAAAGCAAGG GATTGCTGTA CGGTTCCATG GAGAAGAAGG CATGGGTCAA
    GGTGTTGTGC GTGAGTGGTT TGATATTCTG TCCAATGAGA TAGTCAATCC TGATTATGCA
    TTGTTTACCC AGTCAGCTGA TGGAACAACT TTTCAGCCTA ATAGCAACTC TTATGTAAAT
    CCTGATCACT TGAACTATTT TCGGTTTGCT GGGCAGATCT TGGGATTAGC GTTGAACCAC
    AGGCAGCTGG TCAATATTTA CTTCACACGA TCCTTCTACA AGCACATTCT TGGTATTCCT
    GTAAATTACC AAGATGTGGC ATCCATTGAT CCAGAATATG CGAAAAATTT GCAATGGATT
    TTAGATAATG ATATAAGTGA TCTGGGTCTA GAACTAACTT TTTCTGTTGA GACTGATGTG
    TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA CCTGGGGGTG GGAGTATTCT TGTGACACAA
    AATAATAAAG CGGAGTACGT CCAGCTTGTT ACTGAACTTC GAATGACAAG AGCCATTCAG
    CCTCAGATCA ATGCTTTTTT ACAGGGCTTT CATATGTTCA TTCCACCCTC CCTCATACAG
    CTTTTTGATG AATATGAATT GGAGCTACTG CTTTCTGGCA TGCCAGAAAT TGATGTGAGT
    GATTGGATAA AAAATACAGA ATACACAAGT GGCTATGAAA GAGAAGATCC AGTTATTCAG
    TGGTTCTGGG AAGTTGTAGA AGACATTACT CAAGAGGAGA GAGTTCTTCT CTTACAGTTT
    GTTACGGGCA GTTCCAGGGT CCCACATGGT GGGTTTGCTA ATATCATGGG TGGAAGTGGA
    TTGCAAAACT TTACAATCGC TGCTGTGCCA TATACTCCAA ATCTTTTACC AACTTCAAGC
    ACATGCATCA ACATGCTCAA GTTACCTGAA TACCCAAGTA AAGAAATACT CAAGGACAGA
    CTTCTTGTGG CACTACATTG TGGCAGCTAT GGTTACACAA TGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337488.1
    CDS645..2792
    Translation

    Target ORF information:

    RefSeq Version NM_001350559.2
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001350559.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTCAACAT TTGTAATAAT GAAGGCCTTA CAGCATCATG GTCAGAGAGA TACAGCACAG 
    ATCCTACTAT TACGAGGAGC CAAATATCTG CCAGATAAAA ATGGAGTAAC TCCTCTGGAT
    TTATGTGTAC AGGGTGGATA TGGAGAGACT TGTGAAGTAT TAATTCAATA TCACCCGAGG
    CTTTTTCAGA CTATTATTCA AATGACACAG AATGAAGACC TCCGAGAAAA CATGTTACGG
    CAAGTTCTGG AGCATTTGTC TCAGCAAAGT GAAAGCCAGT ACCTAAAGAT TCTAACAAGC
    CTTGCTGAAG TTGCTACAAC AAATGGTCAT AAACTGCTTA GCCTCTCTAG CAATTATGAT
    GCTCAAATGA AGAGCCTTTT AAGGATTGTG AGAATGTTTT GTCACGTCTT TCGAATTGGT
    CCATCCTCCC CCAGTAATGG AATTGATATG GGCTACAATG GGAATAAAAC TCCAAGAAGC
    CAGGTGTTCA AGCCTCTGGA ATTGCTTTGG CACTCGTTAG ATGAATGGCT AGTTTTAATA
    GCCACAGAAT TGATGAAAAA CAAAAGAGAC TCAACAGAGA TCACTTCTAT TTTACTGAAA
    CAAAAAGGCC AAGATCAAGA TGCTGCTTCC ATTCCTCCAT TTGAACCTCC AGGACCTGGG
    AGCTATGAAA ATCTGTCCAC TGGCACAAGG GAATCTAAAC CAGATGCTCT TGCAGGGAGA
    CAGGAAGCCA GTGCAGATTG TCAGGATGTT ATTTCTATGA CAGCTAACCG GCTAAGTGCT
    GTCATTCAAG CTTTTTACAT GTGCTGTTCT TGTCAGATGC CTCCGGGAAT GACTTCACCT
    CGTTTCATTG AATTTGTCTG CAAACATGAT GAAGTTTTAA AATGCTTTGT TAATAGAAAT
    CCCAAAATTA TATTTGACCA CTTTCACTTT CTCCTTGAAT GTCCTGAGTT GATGTCAAGA
    TTCATGCATA TCATAAAAGC ACAGCCTTTT AAAGATCGCT GTGAATGGTT CTATGAACAT
    TTGCATTCAG GACAGCCAGA TTCAGATATG GTGCACAGGC CAGTGAATGA AAATGATATC
    CTGCTGGTTC ACAGAGATTC TATTTTTAGG AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT
    TGTGCAAAGC TAAAGCAAGG GATTGCTGTA CGGTTCCATG GAGAAGAAGG CATGGGTCAA
    GGTGTTGTGC GTGAGTGGTT TGATATTCTG TCCAATGAGA TAGTCAATCC TGATTATGCA
    TTGTTTACCC AGTCAGCTGA TGGAACAACT TTTCAGCCTA ATAGCAACTC TTATGTAAAT
    CCTGATCACT TGAACTATTT TCGGTTTGCT GGGCAGATCT TGGGATTAGC GTTGAACCAC
    AGGCAGCTGG TCAATATTTA CTTCACACGA TCCTTCTACA AGCACATTCT TGGTATTCCT
    GTAAATTACC AAGATGTGGC ATCCATTGAT CCAGAATATG CGAAAAATTT GCAATGGATT
    TTAGATAATG ATATAAGTGA TCTGGGTCTA GAACTAACTT TTTCTGTTGA GACTGATGTG
    TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA CCTGGGGGTG GGAGTATTCT TGTGACACAA
    AATAATAAAG CGGAGTACGT CCAGCTTGTT ACTGAACTTC GAATGACAAG AGCCATTCAG
    CCTCAGATCA ATGCTTTTTT ACAGGGCTTT CATATGTTCA TTCCACCCTC CCTCATACAG
    CTTTTTGATG AATATGAATT GGAGCTACTG CTTTCTGGCA TGCCAGAAAT TGATGTGAGT
    GATTGGATAA AAAATACAGA ATACACAAGT GGCTATGAAA GAGAAGATCC AGTTATTCAG
    TGGTTCTGGG AAGTTGTAGA AGACATTACT CAAGAGGAGA GAGTTCTTCT CTTACAGTTT
    GTTACGGGCA GTTCCAGGGT CCCACATGGT GGGTTTGCTA ATATCATGGG TGGAAGTGGA
    TTGCAAAACT TTACAATCGC TGCTGTGCCA TATACTCCAA ATCTTTTACC AACTTCAAGC
    ACATGCATCA ACATGCTCAA GTTACCTGAA TACCCAAGTA AAGAAATACT CAAGGACAGA
    CTTCTTGTGG CACTACATTG TGGCAGCTAT GGTTACACAA TGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu40896
    Clone ID Related Accession (Same CDS sequence) NM_001350558.1 , NM_001350557.2 , XM_017011119.1 , XM_017011119.2 , NM_001350558.2 , NM_001321084.1 , NM_001321084.2 , XM_017011118.1 , NM_001350557.1
    Accession Version XM_017011119.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2226bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HACE1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
    ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT
    CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT
    GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA
    ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC
    CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC
    CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC
    CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT
    CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG
    AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT
    GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC
    ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT
    GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA
    GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA
    GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT
    CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA
    TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT
    CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT
    GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA
    GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA
    GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA
    GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA
    GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT
    AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG
    GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG
    CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG
    AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT
    TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG
    AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA
    ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT
    CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG
    CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA
    GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA
    GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT
    ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT
    CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA
    GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG
    GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016866608.1
    CDS485..2710
    Translation

    Target ORF information:

    RefSeq Version XM_017011119.1
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017011119.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
    ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT
    CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT
    GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA
    ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC
    CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC
    CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC
    CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT
    CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG
    AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT
    GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC
    ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT
    GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA
    GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA
    GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT
    CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA
    TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT
    CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT
    GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA
    GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA
    GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA
    GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA
    GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT
    AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG
    GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG
    CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG
    AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT
    TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG
    AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA
    ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT
    CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG
    CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA
    GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA
    GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT
    ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT
    CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA
    GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG
    GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu40896
    Clone ID Related Accession (Same CDS sequence) NM_001350558.1 , NM_001350557.2 , XM_017011119.1 , XM_017011119.2 , NM_001350558.2 , NM_001321084.1 , NM_001321084.2 , XM_017011118.1 , NM_001350557.1
    Accession Version XM_017011119.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2226bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HACE1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017011119.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
    ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT
    CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT
    GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA
    ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC
    CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC
    CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC
    CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT
    CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG
    AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT
    GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC
    ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT
    GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA
    GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA
    GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT
    CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA
    TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT
    CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT
    GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA
    GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA
    GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA
    GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA
    GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT
    AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG
    GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG
    CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG
    AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT
    TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG
    AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA
    ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT
    CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG
    CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA
    GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA
    GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT
    ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT
    CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA
    GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG
    GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016866608.1
    CDS788..3013
    Translation

    Target ORF information:

    RefSeq Version XM_017011119.2
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017011119.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
    ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT
    CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT
    GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA
    ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC
    CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC
    CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC
    CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT
    CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG
    AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT
    GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC
    ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT
    GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA
    GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA
    GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT
    CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA
    TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT
    CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT
    GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA
    GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA
    GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA
    GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA
    GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT
    AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG
    GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG
    CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG
    AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT
    TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG
    AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA
    ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT
    CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG
    CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA
    GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA
    GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT
    ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT
    CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA
    GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG
    GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu40896
    Clone ID Related Accession (Same CDS sequence) NM_001350558.1 , NM_001350557.2 , XM_017011119.1 , XM_017011119.2 , NM_001350558.2 , NM_001321084.1 , NM_001321084.2 , XM_017011118.1 , NM_001350557.1
    Accession Version XM_017011118.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2226bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HACE1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
    ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT
    CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT
    GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA
    ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC
    CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC
    CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC
    CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT
    CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG
    AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT
    GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC
    ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT
    GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA
    GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA
    GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT
    CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA
    TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT
    CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT
    GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA
    GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA
    GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA
    GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA
    GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT
    AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG
    GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG
    CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG
    AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT
    TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG
    AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA
    ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT
    CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG
    CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA
    GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA
    GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT
    ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT
    CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA
    GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG
    GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016866607.1
    CDS202..2427
    Translation

    Target ORF information:

    RefSeq Version XM_017011118.1
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017011118.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
    ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT
    CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT
    GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA
    ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC
    CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC
    CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC
    CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT
    CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG
    AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT
    GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC
    ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT
    GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA
    GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA
    GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT
    CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA
    TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT
    CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT
    GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA
    GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA
    GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA
    GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA
    GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT
    AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG
    GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG
    CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG
    AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT
    TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG
    AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA
    ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT
    CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG
    CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA
    GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA
    GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT
    ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT
    CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA
    GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG
    GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu70566
    Clone ID Related Accession (Same CDS sequence) NM_001350559.1 , NM_001350559.2 , XM_017011122.1
    Accession Version XM_017011122.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2148bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HACE1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTCAACAT TTGTAATAAT GAAGGCCTTA CAGCATCATG GTCAGAGAGA TACAGCACAG 
    ATCCTACTAT TACGAGGAGC CAAATATCTG CCAGATAAAA ATGGAGTAAC TCCTCTGGAT
    TTATGTGTAC AGGGTGGATA TGGAGAGACT TGTGAAGTAT TAATTCAATA TCACCCGAGG
    CTTTTTCAGA CTATTATTCA AATGACACAG AATGAAGACC TCCGAGAAAA CATGTTACGG
    CAAGTTCTGG AGCATTTGTC TCAGCAAAGT GAAAGCCAGT ACCTAAAGAT TCTAACAAGC
    CTTGCTGAAG TTGCTACAAC AAATGGTCAT AAACTGCTTA GCCTCTCTAG CAATTATGAT
    GCTCAAATGA AGAGCCTTTT AAGGATTGTG AGAATGTTTT GTCACGTCTT TCGAATTGGT
    CCATCCTCCC CCAGTAATGG AATTGATATG GGCTACAATG GGAATAAAAC TCCAAGAAGC
    CAGGTGTTCA AGCCTCTGGA ATTGCTTTGG CACTCGTTAG ATGAATGGCT AGTTTTAATA
    GCCACAGAAT TGATGAAAAA CAAAAGAGAC TCAACAGAGA TCACTTCTAT TTTACTGAAA
    CAAAAAGGCC AAGATCAAGA TGCTGCTTCC ATTCCTCCAT TTGAACCTCC AGGACCTGGG
    AGCTATGAAA ATCTGTCCAC TGGCACAAGG GAATCTAAAC CAGATGCTCT TGCAGGGAGA
    CAGGAAGCCA GTGCAGATTG TCAGGATGTT ATTTCTATGA CAGCTAACCG GCTAAGTGCT
    GTCATTCAAG CTTTTTACAT GTGCTGTTCT TGTCAGATGC CTCCGGGAAT GACTTCACCT
    CGTTTCATTG AATTTGTCTG CAAACATGAT GAAGTTTTAA AATGCTTTGT TAATAGAAAT
    CCCAAAATTA TATTTGACCA CTTTCACTTT CTCCTTGAAT GTCCTGAGTT GATGTCAAGA
    TTCATGCATA TCATAAAAGC ACAGCCTTTT AAAGATCGCT GTGAATGGTT CTATGAACAT
    TTGCATTCAG GACAGCCAGA TTCAGATATG GTGCACAGGC CAGTGAATGA AAATGATATC
    CTGCTGGTTC ACAGAGATTC TATTTTTAGG AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT
    TGTGCAAAGC TAAAGCAAGG GATTGCTGTA CGGTTCCATG GAGAAGAAGG CATGGGTCAA
    GGTGTTGTGC GTGAGTGGTT TGATATTCTG TCCAATGAGA TAGTCAATCC TGATTATGCA
    TTGTTTACCC AGTCAGCTGA TGGAACAACT TTTCAGCCTA ATAGCAACTC TTATGTAAAT
    CCTGATCACT TGAACTATTT TCGGTTTGCT GGGCAGATCT TGGGATTAGC GTTGAACCAC
    AGGCAGCTGG TCAATATTTA CTTCACACGA TCCTTCTACA AGCACATTCT TGGTATTCCT
    GTAAATTACC AAGATGTGGC ATCCATTGAT CCAGAATATG CGAAAAATTT GCAATGGATT
    TTAGATAATG ATATAAGTGA TCTGGGTCTA GAACTAACTT TTTCTGTTGA GACTGATGTG
    TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA CCTGGGGGTG GGAGTATTCT TGTGACACAA
    AATAATAAAG CGGAGTACGT CCAGCTTGTT ACTGAACTTC GAATGACAAG AGCCATTCAG
    CCTCAGATCA ATGCTTTTTT ACAGGGCTTT CATATGTTCA TTCCACCCTC CCTCATACAG
    CTTTTTGATG AATATGAATT GGAGCTACTG CTTTCTGGCA TGCCAGAAAT TGATGTGAGT
    GATTGGATAA AAAATACAGA ATACACAAGT GGCTATGAAA GAGAAGATCC AGTTATTCAG
    TGGTTCTGGG AAGTTGTAGA AGACATTACT CAAGAGGAGA GAGTTCTTCT CTTACAGTTT
    GTTACGGGCA GTTCCAGGGT CCCACATGGT GGGTTTGCTA ATATCATGGG TGGAAGTGGA
    TTGCAAAACT TTACAATCGC TGCTGTGCCA TATACTCCAA ATCTTTTACC AACTTCAAGC
    ACATGCATCA ACATGCTCAA GTTACCTGAA TACCCAAGTA AAGAAATACT CAAGGACAGA
    CTTCTTGTGG CACTACATTG TGGCAGCTAT GGTTACACAA TGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016866611.1
    CDS397..2544
    Translation

    Target ORF information:

    RefSeq Version XM_017011122.1
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017011122.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTCAACAT TTGTAATAAT GAAGGCCTTA CAGCATCATG GTCAGAGAGA TACAGCACAG 
    ATCCTACTAT TACGAGGAGC CAAATATCTG CCAGATAAAA ATGGAGTAAC TCCTCTGGAT
    TTATGTGTAC AGGGTGGATA TGGAGAGACT TGTGAAGTAT TAATTCAATA TCACCCGAGG
    CTTTTTCAGA CTATTATTCA AATGACACAG AATGAAGACC TCCGAGAAAA CATGTTACGG
    CAAGTTCTGG AGCATTTGTC TCAGCAAAGT GAAAGCCAGT ACCTAAAGAT TCTAACAAGC
    CTTGCTGAAG TTGCTACAAC AAATGGTCAT AAACTGCTTA GCCTCTCTAG CAATTATGAT
    GCTCAAATGA AGAGCCTTTT AAGGATTGTG AGAATGTTTT GTCACGTCTT TCGAATTGGT
    CCATCCTCCC CCAGTAATGG AATTGATATG GGCTACAATG GGAATAAAAC TCCAAGAAGC
    CAGGTGTTCA AGCCTCTGGA ATTGCTTTGG CACTCGTTAG ATGAATGGCT AGTTTTAATA
    GCCACAGAAT TGATGAAAAA CAAAAGAGAC TCAACAGAGA TCACTTCTAT TTTACTGAAA
    CAAAAAGGCC AAGATCAAGA TGCTGCTTCC ATTCCTCCAT TTGAACCTCC AGGACCTGGG
    AGCTATGAAA ATCTGTCCAC TGGCACAAGG GAATCTAAAC CAGATGCTCT TGCAGGGAGA
    CAGGAAGCCA GTGCAGATTG TCAGGATGTT ATTTCTATGA CAGCTAACCG GCTAAGTGCT
    GTCATTCAAG CTTTTTACAT GTGCTGTTCT TGTCAGATGC CTCCGGGAAT GACTTCACCT
    CGTTTCATTG AATTTGTCTG CAAACATGAT GAAGTTTTAA AATGCTTTGT TAATAGAAAT
    CCCAAAATTA TATTTGACCA CTTTCACTTT CTCCTTGAAT GTCCTGAGTT GATGTCAAGA
    TTCATGCATA TCATAAAAGC ACAGCCTTTT AAAGATCGCT GTGAATGGTT CTATGAACAT
    TTGCATTCAG GACAGCCAGA TTCAGATATG GTGCACAGGC CAGTGAATGA AAATGATATC
    CTGCTGGTTC ACAGAGATTC TATTTTTAGG AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT
    TGTGCAAAGC TAAAGCAAGG GATTGCTGTA CGGTTCCATG GAGAAGAAGG CATGGGTCAA
    GGTGTTGTGC GTGAGTGGTT TGATATTCTG TCCAATGAGA TAGTCAATCC TGATTATGCA
    TTGTTTACCC AGTCAGCTGA TGGAACAACT TTTCAGCCTA ATAGCAACTC TTATGTAAAT
    CCTGATCACT TGAACTATTT TCGGTTTGCT GGGCAGATCT TGGGATTAGC GTTGAACCAC
    AGGCAGCTGG TCAATATTTA CTTCACACGA TCCTTCTACA AGCACATTCT TGGTATTCCT
    GTAAATTACC AAGATGTGGC ATCCATTGAT CCAGAATATG CGAAAAATTT GCAATGGATT
    TTAGATAATG ATATAAGTGA TCTGGGTCTA GAACTAACTT TTTCTGTTGA GACTGATGTG
    TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA CCTGGGGGTG GGAGTATTCT TGTGACACAA
    AATAATAAAG CGGAGTACGT CCAGCTTGTT ACTGAACTTC GAATGACAAG AGCCATTCAG
    CCTCAGATCA ATGCTTTTTT ACAGGGCTTT CATATGTTCA TTCCACCCTC CCTCATACAG
    CTTTTTGATG AATATGAATT GGAGCTACTG CTTTCTGGCA TGCCAGAAAT TGATGTGAGT
    GATTGGATAA AAAATACAGA ATACACAAGT GGCTATGAAA GAGAAGATCC AGTTATTCAG
    TGGTTCTGGG AAGTTGTAGA AGACATTACT CAAGAGGAGA GAGTTCTTCT CTTACAGTTT
    GTTACGGGCA GTTCCAGGGT CCCACATGGT GGGTTTGCTA ATATCATGGG TGGAAGTGGA
    TTGCAAAACT TTACAATCGC TGCTGTGCCA TATACTCCAA ATCTTTTACC AACTTCAAGC
    ACATGCATCA ACATGCTCAA GTTACCTGAA TACCCAAGTA AAGAAATACT CAAGGACAGA
    CTTCTTGTGG CACTACATTG TGGCAGCTAT GGTTACACAA TGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu101567
    Clone ID Related Accession (Same CDS sequence) NM_001350555.1 , NM_001350555.2
    Accession Version NM_001350555.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2439bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-22
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HACE1 isoform f
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357315.14, AL513472.9 and AL590402.10. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.14745.1, SRR1803613.279335.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGAGAGAG CGATGGAGCA ACTCAACCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGTTGC CCGAGGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTCGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC GGTGGAATGC
    TTGGTTTTGC TGTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGCTGTACA
    CCCCTTCATT TGGCAGCAAG AAATGGTCAT GGTCAGAGAG ATACAGCACA GATCCTACTA
    TTACGAGGAG CCAAATATCT GCCAGATAAA AATGGAGTAA CTCCTCTGGA TTTATGTGTA
    CAGGGTGGAT ATGGAGAGAC TTGTGAAGTA TTAATTCAAT ATCACCCGAG GCTTTTTCAG
    ACTATTATTC AAATGACACA GAATGAAGAC CTCCGAGAAA ACATGTTACG GCAAGTTCTG
    GAGCATTTGT CTCAGCAAAG TGAAAGCCAG TACCTAAAGA TTCTAACAAG CCTTGCTGAA
    GTTGCTACAA CAAATGGTCA TAAACTGCTT AGCCTCTCTA GCAATTATGA TGCTCAAATG
    AAGAGCCTTT TAAGGATTGT GAGAATGTTT TGTCACGTCT TTCGAATTGG TCCATCCTCC
    CCCAGTAATG GAATTGATAT GGGCTACAAT GGGAATAAAA CTCCAAGAAG CCAGGTGTTC
    AAGCCTCTGG AATTGCTTTG GCACTCGTTA GATGAATGGC TAGTTTTAAT AGCCACAGAA
    TTGATGAAAA ACAAAAGAGA CTCAACAGAG ATCACTTCTA TTTTACTGAA ACAAAAAGGC
    CAAGATCAAG ATGCTGCTTC CATTCCTCCA TTTGAACCTC CAGGACCTGG GAGCTATGAA
    AATCTGTCCA CTGGCACAAG GGAATCTAAA CCAGATGCTC TTGCAGGGAG ACAGGAAGCC
    AGTGCAGATT GTCAGGATGT TATTTCTATG ACAGCTAACC GGCTAAGTGC TGTCATTCAA
    GCTTTTTACA TGTGCTGTTC TTGTCAGATG CCTCCGGGAA TGACTTCACC TCGTTTCATT
    GAATTTGTCT GCAAACATGA TGAAGTTTTA AAATGCTTTG TTAATAGAAA TCCCAAAATT
    ATATTTGACC ACTTTCACTT TCTCCTTGAA TGTCCTGAGT TGATGTCAAG ATTCATGCAT
    ATCATAAAAG CACAGCCTTT TAAAGATCGC TGTGAATGGT TCTATGAACA TTTGCATTCA
    GGACAGCCAG ATTCAGATAT GGTGCACAGG CCAGTGAATG AAAATGATAT CCTGCTGGTT
    CACAGAGATT CTATTTTTAG GAGTAGCTGT GAAGTTGTGT CAAAAGCAAA TTGTGCAAAG
    CTAAAGCAAG GGATTGCTGT ACGGTTCCAT GGAGAAGAAG GCATGGGTCA AGGTGTTGTG
    CGTGAGTGGT TTGATATTCT GTCCAATGAG ATAGTCAATC CTGATTATGC ATTGTTTACC
    CAGTCAGCTG ATGGAACAAC TTTTCAGCCT AATAGCAACT CTTATGTAAA TCCTGATCAC
    TTGAACTATT TTCGGTTTGC TGGGCAGATC TTGGGATTAG CGTTGAACCA CAGGCAGCTG
    GTCAATATTT ACTTCACACG ATCCTTCTAC AAGCACATTC TTGGTATTCC TGTAAATTAC
    CAAGATGTGG CATCCATTGA TCCAGAATAT GCGAAAAATT TGCAATGGAT TTTAGATAAT
    GATATAAGTG ATCTGGGTCT AGAACTAACT TTTTCTGTTG AGACTGATGT GTTTGGAGCA
    ATGGAAGAGG TGCCTTTGAA ACCTGGGGGT GGGAGTATTC TTGTGACACA AAATAATAAA
    GCGGAGTACG TCCAGCTTGT TACTGAACTT CGAATGACAA GAGCCATTCA GCCTCAGATC
    AATGCTTTTT TACAGGGCTT TCATATGTTC ATTCCACCCT CCCTCATACA GCTTTTTGAT
    GAATATGAAT TGGAGCTACT GCTTTCTGGC ATGCCAGAAA TTGATGTGAG TGATTGGATA
    AAAAATACAG AATACACAAG TGGCTATGAA AGAGAAGATC CAGTTATTCA GTGGTTCTGG
    GAAGTTGTAG AAGACATTAC TCAAGAGGAG AGAGTTCTTC TCTTACAGTT TGTTACGGGC
    AGTTCCAGGG TCCCACATGG TGGGTTTGCT AATATCATGG GTGGAAGTGG ATTGCAAAAC
    TTTACAATCG CTGCTGTGCC ATATACTCCA AATCTTTTAC CAACTTCAAG CACATGCATC
    AACATGCTCA AGTTACCTGA ATACCCAAGT AAAGAAATAC TCAAGGACAG ACTTCTTGTG
    GCACTACATT GTGGCAGCTA TGGTTACACA ATGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337484.1
    CDS278..2716
    Translation

    Target ORF information:

    RefSeq Version NM_001350555.1
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001350555.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGAGAGAG CGATGGAGCA ACTCAACCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGTTGC CCGAGGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTCGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC GGTGGAATGC
    TTGGTTTTGC TGTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGCTGTACA
    CCCCTTCATT TGGCAGCAAG AAATGGTCAT GGTCAGAGAG ATACAGCACA GATCCTACTA
    TTACGAGGAG CCAAATATCT GCCAGATAAA AATGGAGTAA CTCCTCTGGA TTTATGTGTA
    CAGGGTGGAT ATGGAGAGAC TTGTGAAGTA TTAATTCAAT ATCACCCGAG GCTTTTTCAG
    ACTATTATTC AAATGACACA GAATGAAGAC CTCCGAGAAA ACATGTTACG GCAAGTTCTG
    GAGCATTTGT CTCAGCAAAG TGAAAGCCAG TACCTAAAGA TTCTAACAAG CCTTGCTGAA
    GTTGCTACAA CAAATGGTCA TAAACTGCTT AGCCTCTCTA GCAATTATGA TGCTCAAATG
    AAGAGCCTTT TAAGGATTGT GAGAATGTTT TGTCACGTCT TTCGAATTGG TCCATCCTCC
    CCCAGTAATG GAATTGATAT GGGCTACAAT GGGAATAAAA CTCCAAGAAG CCAGGTGTTC
    AAGCCTCTGG AATTGCTTTG GCACTCGTTA GATGAATGGC TAGTTTTAAT AGCCACAGAA
    TTGATGAAAA ACAAAAGAGA CTCAACAGAG ATCACTTCTA TTTTACTGAA ACAAAAAGGC
    CAAGATCAAG ATGCTGCTTC CATTCCTCCA TTTGAACCTC CAGGACCTGG GAGCTATGAA
    AATCTGTCCA CTGGCACAAG GGAATCTAAA CCAGATGCTC TTGCAGGGAG ACAGGAAGCC
    AGTGCAGATT GTCAGGATGT TATTTCTATG ACAGCTAACC GGCTAAGTGC TGTCATTCAA
    GCTTTTTACA TGTGCTGTTC TTGTCAGATG CCTCCGGGAA TGACTTCACC TCGTTTCATT
    GAATTTGTCT GCAAACATGA TGAAGTTTTA AAATGCTTTG TTAATAGAAA TCCCAAAATT
    ATATTTGACC ACTTTCACTT TCTCCTTGAA TGTCCTGAGT TGATGTCAAG ATTCATGCAT
    ATCATAAAAG CACAGCCTTT TAAAGATCGC TGTGAATGGT TCTATGAACA TTTGCATTCA
    GGACAGCCAG ATTCAGATAT GGTGCACAGG CCAGTGAATG AAAATGATAT CCTGCTGGTT
    CACAGAGATT CTATTTTTAG GAGTAGCTGT GAAGTTGTGT CAAAAGCAAA TTGTGCAAAG
    CTAAAGCAAG GGATTGCTGT ACGGTTCCAT GGAGAAGAAG GCATGGGTCA AGGTGTTGTG
    CGTGAGTGGT TTGATATTCT GTCCAATGAG ATAGTCAATC CTGATTATGC ATTGTTTACC
    CAGTCAGCTG ATGGAACAAC TTTTCAGCCT AATAGCAACT CTTATGTAAA TCCTGATCAC
    TTGAACTATT TTCGGTTTGC TGGGCAGATC TTGGGATTAG CGTTGAACCA CAGGCAGCTG
    GTCAATATTT ACTTCACACG ATCCTTCTAC AAGCACATTC TTGGTATTCC TGTAAATTAC
    CAAGATGTGG CATCCATTGA TCCAGAATAT GCGAAAAATT TGCAATGGAT TTTAGATAAT
    GATATAAGTG ATCTGGGTCT AGAACTAACT TTTTCTGTTG AGACTGATGT GTTTGGAGCA
    ATGGAAGAGG TGCCTTTGAA ACCTGGGGGT GGGAGTATTC TTGTGACACA AAATAATAAA
    GCGGAGTACG TCCAGCTTGT TACTGAACTT CGAATGACAA GAGCCATTCA GCCTCAGATC
    AATGCTTTTT TACAGGGCTT TCATATGTTC ATTCCACCCT CCCTCATACA GCTTTTTGAT
    GAATATGAAT TGGAGCTACT GCTTTCTGGC ATGCCAGAAA TTGATGTGAG TGATTGGATA
    AAAAATACAG AATACACAAG TGGCTATGAA AGAGAAGATC CAGTTATTCA GTGGTTCTGG
    GAAGTTGTAG AAGACATTAC TCAAGAGGAG AGAGTTCTTC TCTTACAGTT TGTTACGGGC
    AGTTCCAGGG TCCCACATGG TGGGTTTGCT AATATCATGG GTGGAAGTGG ATTGCAAAAC
    TTTACAATCG CTGCTGTGCC ATATACTCCA AATCTTTTAC CAACTTCAAG CACATGCATC
    AACATGCTCA AGTTACCTGA ATACCCAAGT AAAGAAATAC TCAAGGACAG ACTTCTTGTG
    GCACTACATT GTGGCAGCTA TGGTTACACA ATGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu101567
    Clone ID Related Accession (Same CDS sequence) NM_001350555.1 , NM_001350555.2
    Accession Version NM_001350555.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2439bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-04
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HACE1 isoform f
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357315.14, AL513472.9 and AL590402.10. On May 31, 2019 this sequence version replaced NM_001350555.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.14745.1, SRR1803613.279335.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGAGAGAG CGATGGAGCA ACTCAACCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGTTGC CCGAGGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTCGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC GGTGGAATGC
    TTGGTTTTGC TGTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGCTGTACA
    CCCCTTCATT TGGCAGCAAG AAATGGTCAT GGTCAGAGAG ATACAGCACA GATCCTACTA
    TTACGAGGAG CCAAATATCT GCCAGATAAA AATGGAGTAA CTCCTCTGGA TTTATGTGTA
    CAGGGTGGAT ATGGAGAGAC TTGTGAAGTA TTAATTCAAT ATCACCCGAG GCTTTTTCAG
    ACTATTATTC AAATGACACA GAATGAAGAC CTCCGAGAAA ACATGTTACG GCAAGTTCTG
    GAGCATTTGT CTCAGCAAAG TGAAAGCCAG TACCTAAAGA TTCTAACAAG CCTTGCTGAA
    GTTGCTACAA CAAATGGTCA TAAACTGCTT AGCCTCTCTA GCAATTATGA TGCTCAAATG
    AAGAGCCTTT TAAGGATTGT GAGAATGTTT TGTCACGTCT TTCGAATTGG TCCATCCTCC
    CCCAGTAATG GAATTGATAT GGGCTACAAT GGGAATAAAA CTCCAAGAAG CCAGGTGTTC
    AAGCCTCTGG AATTGCTTTG GCACTCGTTA GATGAATGGC TAGTTTTAAT AGCCACAGAA
    TTGATGAAAA ACAAAAGAGA CTCAACAGAG ATCACTTCTA TTTTACTGAA ACAAAAAGGC
    CAAGATCAAG ATGCTGCTTC CATTCCTCCA TTTGAACCTC CAGGACCTGG GAGCTATGAA
    AATCTGTCCA CTGGCACAAG GGAATCTAAA CCAGATGCTC TTGCAGGGAG ACAGGAAGCC
    AGTGCAGATT GTCAGGATGT TATTTCTATG ACAGCTAACC GGCTAAGTGC TGTCATTCAA
    GCTTTTTACA TGTGCTGTTC TTGTCAGATG CCTCCGGGAA TGACTTCACC TCGTTTCATT
    GAATTTGTCT GCAAACATGA TGAAGTTTTA AAATGCTTTG TTAATAGAAA TCCCAAAATT
    ATATTTGACC ACTTTCACTT TCTCCTTGAA TGTCCTGAGT TGATGTCAAG ATTCATGCAT
    ATCATAAAAG CACAGCCTTT TAAAGATCGC TGTGAATGGT TCTATGAACA TTTGCATTCA
    GGACAGCCAG ATTCAGATAT GGTGCACAGG CCAGTGAATG AAAATGATAT CCTGCTGGTT
    CACAGAGATT CTATTTTTAG GAGTAGCTGT GAAGTTGTGT CAAAAGCAAA TTGTGCAAAG
    CTAAAGCAAG GGATTGCTGT ACGGTTCCAT GGAGAAGAAG GCATGGGTCA AGGTGTTGTG
    CGTGAGTGGT TTGATATTCT GTCCAATGAG ATAGTCAATC CTGATTATGC ATTGTTTACC
    CAGTCAGCTG ATGGAACAAC TTTTCAGCCT AATAGCAACT CTTATGTAAA TCCTGATCAC
    TTGAACTATT TTCGGTTTGC TGGGCAGATC TTGGGATTAG CGTTGAACCA CAGGCAGCTG
    GTCAATATTT ACTTCACACG ATCCTTCTAC AAGCACATTC TTGGTATTCC TGTAAATTAC
    CAAGATGTGG CATCCATTGA TCCAGAATAT GCGAAAAATT TGCAATGGAT TTTAGATAAT
    GATATAAGTG ATCTGGGTCT AGAACTAACT TTTTCTGTTG AGACTGATGT GTTTGGAGCA
    ATGGAAGAGG TGCCTTTGAA ACCTGGGGGT GGGAGTATTC TTGTGACACA AAATAATAAA
    GCGGAGTACG TCCAGCTTGT TACTGAACTT CGAATGACAA GAGCCATTCA GCCTCAGATC
    AATGCTTTTT TACAGGGCTT TCATATGTTC ATTCCACCCT CCCTCATACA GCTTTTTGAT
    GAATATGAAT TGGAGCTACT GCTTTCTGGC ATGCCAGAAA TTGATGTGAG TGATTGGATA
    AAAAATACAG AATACACAAG TGGCTATGAA AGAGAAGATC CAGTTATTCA GTGGTTCTGG
    GAAGTTGTAG AAGACATTAC TCAAGAGGAG AGAGTTCTTC TCTTACAGTT TGTTACGGGC
    AGTTCCAGGG TCCCACATGG TGGGTTTGCT AATATCATGG GTGGAAGTGG ATTGCAAAAC
    TTTACAATCG CTGCTGTGCC ATATACTCCA AATCTTTTAC CAACTTCAAG CACATGCATC
    AACATGCTCA AGTTACCTGA ATACCCAAGT AAAGAAATAC TCAAGGACAG ACTTCTTGTG
    GCACTACATT GTGGCAGCTA TGGTTACACA ATGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337484.1
    CDS278..2716
    Translation

    Target ORF information:

    RefSeq Version NM_001350555.2
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001350555.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGAGAGAG CGATGGAGCA ACTCAACCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGTTGC CCGAGGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTCGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC GGTGGAATGC
    TTGGTTTTGC TGTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGCTGTACA
    CCCCTTCATT TGGCAGCAAG AAATGGTCAT GGTCAGAGAG ATACAGCACA GATCCTACTA
    TTACGAGGAG CCAAATATCT GCCAGATAAA AATGGAGTAA CTCCTCTGGA TTTATGTGTA
    CAGGGTGGAT ATGGAGAGAC TTGTGAAGTA TTAATTCAAT ATCACCCGAG GCTTTTTCAG
    ACTATTATTC AAATGACACA GAATGAAGAC CTCCGAGAAA ACATGTTACG GCAAGTTCTG
    GAGCATTTGT CTCAGCAAAG TGAAAGCCAG TACCTAAAGA TTCTAACAAG CCTTGCTGAA
    GTTGCTACAA CAAATGGTCA TAAACTGCTT AGCCTCTCTA GCAATTATGA TGCTCAAATG
    AAGAGCCTTT TAAGGATTGT GAGAATGTTT TGTCACGTCT TTCGAATTGG TCCATCCTCC
    CCCAGTAATG GAATTGATAT GGGCTACAAT GGGAATAAAA CTCCAAGAAG CCAGGTGTTC
    AAGCCTCTGG AATTGCTTTG GCACTCGTTA GATGAATGGC TAGTTTTAAT AGCCACAGAA
    TTGATGAAAA ACAAAAGAGA CTCAACAGAG ATCACTTCTA TTTTACTGAA ACAAAAAGGC
    CAAGATCAAG ATGCTGCTTC CATTCCTCCA TTTGAACCTC CAGGACCTGG GAGCTATGAA
    AATCTGTCCA CTGGCACAAG GGAATCTAAA CCAGATGCTC TTGCAGGGAG ACAGGAAGCC
    AGTGCAGATT GTCAGGATGT TATTTCTATG ACAGCTAACC GGCTAAGTGC TGTCATTCAA
    GCTTTTTACA TGTGCTGTTC TTGTCAGATG CCTCCGGGAA TGACTTCACC TCGTTTCATT
    GAATTTGTCT GCAAACATGA TGAAGTTTTA AAATGCTTTG TTAATAGAAA TCCCAAAATT
    ATATTTGACC ACTTTCACTT TCTCCTTGAA TGTCCTGAGT TGATGTCAAG ATTCATGCAT
    ATCATAAAAG CACAGCCTTT TAAAGATCGC TGTGAATGGT TCTATGAACA TTTGCATTCA
    GGACAGCCAG ATTCAGATAT GGTGCACAGG CCAGTGAATG AAAATGATAT CCTGCTGGTT
    CACAGAGATT CTATTTTTAG GAGTAGCTGT GAAGTTGTGT CAAAAGCAAA TTGTGCAAAG
    CTAAAGCAAG GGATTGCTGT ACGGTTCCAT GGAGAAGAAG GCATGGGTCA AGGTGTTGTG
    CGTGAGTGGT TTGATATTCT GTCCAATGAG ATAGTCAATC CTGATTATGC ATTGTTTACC
    CAGTCAGCTG ATGGAACAAC TTTTCAGCCT AATAGCAACT CTTATGTAAA TCCTGATCAC
    TTGAACTATT TTCGGTTTGC TGGGCAGATC TTGGGATTAG CGTTGAACCA CAGGCAGCTG
    GTCAATATTT ACTTCACACG ATCCTTCTAC AAGCACATTC TTGGTATTCC TGTAAATTAC
    CAAGATGTGG CATCCATTGA TCCAGAATAT GCGAAAAATT TGCAATGGAT TTTAGATAAT
    GATATAAGTG ATCTGGGTCT AGAACTAACT TTTTCTGTTG AGACTGATGT GTTTGGAGCA
    ATGGAAGAGG TGCCTTTGAA ACCTGGGGGT GGGAGTATTC TTGTGACACA AAATAATAAA
    GCGGAGTACG TCCAGCTTGT TACTGAACTT CGAATGACAA GAGCCATTCA GCCTCAGATC
    AATGCTTTTT TACAGGGCTT TCATATGTTC ATTCCACCCT CCCTCATACA GCTTTTTGAT
    GAATATGAAT TGGAGCTACT GCTTTCTGGC ATGCCAGAAA TTGATGTGAG TGATTGGATA
    AAAAATACAG AATACACAAG TGGCTATGAA AGAGAAGATC CAGTTATTCA GTGGTTCTGG
    GAAGTTGTAG AAGACATTAC TCAAGAGGAG AGAGTTCTTC TCTTACAGTT TGTTACGGGC
    AGTTCCAGGG TCCCACATGG TGGGTTTGCT AATATCATGG GTGGAAGTGG ATTGCAAAAC
    TTTACAATCG CTGCTGTGCC ATATACTCCA AATCTTTTAC CAACTTCAAG CACATGCATC
    AACATGCTCA AGTTACCTGA ATACCCAAGT AAAGAAATAC TCAAGGACAG ACTTCTTGTG
    GCACTACATT GTGGCAGCTA TGGTTACACA ATGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96713
    Clone ID Related Accession (Same CDS sequence) NM_001350554.1 , XM_017011114.2 , XM_017011114.1 , NM_001350554.2
    Accession Version NM_001350554.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2496bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-22
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HACE1 isoform e
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357315.14, AL513472.9 and AL590402.10. On Apr 20, 2017 this sequence version replaced XM_017011113.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.96954.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCCAATGG TTATGGCTGA TCAACACAGG TCTGTTTCTG AACTACTATC AAATTCAAAA 
    TTTGATGTCA ATTATGCATT CGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT
    TGTGGATCGG TGGAATGCTT GGTTTTGCTG TTAAAGAAAG GAGCAAATCC TAACTATCAA
    GATATTTCAG GCTGTACACC CCTTCATTTG GCAGCAAGAA ATGGGCAGAA GAAATGTATG
    AGTAAATTAT TAGAATATAG CGCTGATGTC AACATTTGTA ATAATGAAGG CCTTACAGCA
    ACAGTGCAGT GCTTGCTAGA CAGTGGTGCT GATATTAACA GGCCAAATGT ATCAGGAGCA
    ACTCCATTGT ACTTTGCTTG CAGTCATGGT CAGAGAGATA CAGCACAGAT CCTACTATTA
    CGAGGAGCCA AATATCTGCC AGATAAAAAT GGAGTAACTC CTCTGGATTT ATGTGTACAG
    GGTGGATATG GAGAGACTTG TGAAGTATTA ATTCAATATC ACCCGAGGCT TTTTCAGACT
    ATTATTCAAA TGACACAGAA TGAAGACCTC CGAGAAAACA TGTTACGGCA AGTTCTGGAG
    CATTTGTCTC AGCAAAGTGA AAGCCAGTAC CTAAAGATTC TAACAAGCCT TGCTGAAGTT
    GCTACAACAA ATGGTCATAA ACTGCTTAGC CTCTCTAGCA ATTATGATGC TCAAATGAAG
    AGCCTTTTAA GGATTGTGAG AATGTTTTGT CACGTCTTTC GAATTGGTCC ATCCTCCCCC
    AGTAATGGAA TTGATATGGG CTACAATGGG AATAAAACTC CAAGAAGCCA GGTGTTCAAG
    CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT GAATGGCTAG TTTTAATAGC CACAGAATTG
    ATGAAAAACA AAAGAGACTC AACAGAGATC ACTTCTATTT TACTGAAACA AAAAGGCCAA
    GATCAAGATG CTGCTTCCAT TCCTCCATTT GAACCTCCAG GACCTGGGAG CTATGAAAAT
    CTGTCCACTG GCACAAGGGA ATCTAAACCA GATGCTCTTG CAGGGAGACA GGAAGCCAGT
    GCAGATTGTC AGGATGTTAT TTCTATGACA GCTAACCGGC TAAGTGCTGT CATTCAAGCT
    TTTTACATGT GCTGTTCTTG TCAGATGCCT CCGGGAATGA CTTCACCTCG TTTCATTGAA
    TTTGTCTGCA AACATGATGA AGTTTTAAAA TGCTTTGTTA ATAGAAATCC CAAAATTATA
    TTTGACCACT TTCACTTTCT CCTTGAATGT CCTGAGTTGA TGTCAAGATT CATGCATATC
    ATAAAAGCAC AGCCTTTTAA AGATCGCTGT GAATGGTTCT ATGAACATTT GCATTCAGGA
    CAGCCAGATT CAGATATGGT GCACAGGCCA GTGAATGAAA ATGATATCCT GCTGGTTCAC
    AGAGATTCTA TTTTTAGGAG TAGCTGTGAA GTTGTGTCAA AAGCAAATTG TGCAAAGCTA
    AAGCAAGGGA TTGCTGTACG GTTCCATGGA GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT
    GAGTGGTTTG ATATTCTGTC CAATGAGATA GTCAATCCTG ATTATGCATT GTTTACCCAG
    TCAGCTGATG GAACAACTTT TCAGCCTAAT AGCAACTCTT ATGTAAATCC TGATCACTTG
    AACTATTTTC GGTTTGCTGG GCAGATCTTG GGATTAGCGT TGAACCACAG GCAGCTGGTC
    AATATTTACT TCACACGATC CTTCTACAAG CACATTCTTG GTATTCCTGT AAATTACCAA
    GATGTGGCAT CCATTGATCC AGAATATGCG AAAAATTTGC AATGGATTTT AGATAATGAT
    ATAAGTGATC TGGGTCTAGA ACTAACTTTT TCTGTTGAGA CTGATGTGTT TGGAGCAATG
    GAAGAGGTGC CTTTGAAACC TGGGGGTGGG AGTATTCTTG TGACACAAAA TAATAAAGCG
    GAGTACGTCC AGCTTGTTAC TGAACTTCGA ATGACAAGAG CCATTCAGCC TCAGATCAAT
    GCTTTTTTAC AGGGCTTTCA TATGTTCATT CCACCCTCCC TCATACAGCT TTTTGATGAA
    TATGAATTGG AGCTACTGCT TTCTGGCATG CCAGAAATTG ATGTGAGTGA TTGGATAAAA
    AATACAGAAT ACACAAGTGG CTATGAAAGA GAAGATCCAG TTATTCAGTG GTTCTGGGAA
    GTTGTAGAAG ACATTACTCA AGAGGAGAGA GTTCTTCTCT TACAGTTTGT TACGGGCAGT
    TCCAGGGTCC CACATGGTGG GTTTGCTAAT ATCATGGGTG GAAGTGGATT GCAAAACTTT
    ACAATCGCTG CTGTGCCATA TACTCCAAAT CTTTTACCAA CTTCAAGCAC ATGCATCAAC
    ATGCTCAAGT TACCTGAATA CCCAAGTAAA GAAATACTCA AGGACAGACT TCTTGTGGCA
    CTACATTGTG GCAGCTATGG TTACACAATG GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337483.1
    CDS438..2933
    Translation

    Target ORF information:

    RefSeq Version NM_001350554.1
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001350554.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCCAATGG TTATGGCTGA TCAACACAGG TCTGTTTCTG AACTACTATC AAATTCAAAA 
    TTTGATGTCA ATTATGCATT CGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT
    TGTGGATCGG TGGAATGCTT GGTTTTGCTG TTAAAGAAAG GAGCAAATCC TAACTATCAA
    GATATTTCAG GCTGTACACC CCTTCATTTG GCAGCAAGAA ATGGGCAGAA GAAATGTATG
    AGTAAATTAT TAGAATATAG CGCTGATGTC AACATTTGTA ATAATGAAGG CCTTACAGCA
    ACAGTGCAGT GCTTGCTAGA CAGTGGTGCT GATATTAACA GGCCAAATGT ATCAGGAGCA
    ACTCCATTGT ACTTTGCTTG CAGTCATGGT CAGAGAGATA CAGCACAGAT CCTACTATTA
    CGAGGAGCCA AATATCTGCC AGATAAAAAT GGAGTAACTC CTCTGGATTT ATGTGTACAG
    GGTGGATATG GAGAGACTTG TGAAGTATTA ATTCAATATC ACCCGAGGCT TTTTCAGACT
    ATTATTCAAA TGACACAGAA TGAAGACCTC CGAGAAAACA TGTTACGGCA AGTTCTGGAG
    CATTTGTCTC AGCAAAGTGA AAGCCAGTAC CTAAAGATTC TAACAAGCCT TGCTGAAGTT
    GCTACAACAA ATGGTCATAA ACTGCTTAGC CTCTCTAGCA ATTATGATGC TCAAATGAAG
    AGCCTTTTAA GGATTGTGAG AATGTTTTGT CACGTCTTTC GAATTGGTCC ATCCTCCCCC
    AGTAATGGAA TTGATATGGG CTACAATGGG AATAAAACTC CAAGAAGCCA GGTGTTCAAG
    CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT GAATGGCTAG TTTTAATAGC CACAGAATTG
    ATGAAAAACA AAAGAGACTC AACAGAGATC ACTTCTATTT TACTGAAACA AAAAGGCCAA
    GATCAAGATG CTGCTTCCAT TCCTCCATTT GAACCTCCAG GACCTGGGAG CTATGAAAAT
    CTGTCCACTG GCACAAGGGA ATCTAAACCA GATGCTCTTG CAGGGAGACA GGAAGCCAGT
    GCAGATTGTC AGGATGTTAT TTCTATGACA GCTAACCGGC TAAGTGCTGT CATTCAAGCT
    TTTTACATGT GCTGTTCTTG TCAGATGCCT CCGGGAATGA CTTCACCTCG TTTCATTGAA
    TTTGTCTGCA AACATGATGA AGTTTTAAAA TGCTTTGTTA ATAGAAATCC CAAAATTATA
    TTTGACCACT TTCACTTTCT CCTTGAATGT CCTGAGTTGA TGTCAAGATT CATGCATATC
    ATAAAAGCAC AGCCTTTTAA AGATCGCTGT GAATGGTTCT ATGAACATTT GCATTCAGGA
    CAGCCAGATT CAGATATGGT GCACAGGCCA GTGAATGAAA ATGATATCCT GCTGGTTCAC
    AGAGATTCTA TTTTTAGGAG TAGCTGTGAA GTTGTGTCAA AAGCAAATTG TGCAAAGCTA
    AAGCAAGGGA TTGCTGTACG GTTCCATGGA GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT
    GAGTGGTTTG ATATTCTGTC CAATGAGATA GTCAATCCTG ATTATGCATT GTTTACCCAG
    TCAGCTGATG GAACAACTTT TCAGCCTAAT AGCAACTCTT ATGTAAATCC TGATCACTTG
    AACTATTTTC GGTTTGCTGG GCAGATCTTG GGATTAGCGT TGAACCACAG GCAGCTGGTC
    AATATTTACT TCACACGATC CTTCTACAAG CACATTCTTG GTATTCCTGT AAATTACCAA
    GATGTGGCAT CCATTGATCC AGAATATGCG AAAAATTTGC AATGGATTTT AGATAATGAT
    ATAAGTGATC TGGGTCTAGA ACTAACTTTT TCTGTTGAGA CTGATGTGTT TGGAGCAATG
    GAAGAGGTGC CTTTGAAACC TGGGGGTGGG AGTATTCTTG TGACACAAAA TAATAAAGCG
    GAGTACGTCC AGCTTGTTAC TGAACTTCGA ATGACAAGAG CCATTCAGCC TCAGATCAAT
    GCTTTTTTAC AGGGCTTTCA TATGTTCATT CCACCCTCCC TCATACAGCT TTTTGATGAA
    TATGAATTGG AGCTACTGCT TTCTGGCATG CCAGAAATTG ATGTGAGTGA TTGGATAAAA
    AATACAGAAT ACACAAGTGG CTATGAAAGA GAAGATCCAG TTATTCAGTG GTTCTGGGAA
    GTTGTAGAAG ACATTACTCA AGAGGAGAGA GTTCTTCTCT TACAGTTTGT TACGGGCAGT
    TCCAGGGTCC CACATGGTGG GTTTGCTAAT ATCATGGGTG GAAGTGGATT GCAAAACTTT
    ACAATCGCTG CTGTGCCATA TACTCCAAAT CTTTTACCAA CTTCAAGCAC ATGCATCAAC
    ATGCTCAAGT TACCTGAATA CCCAAGTAAA GAAATACTCA AGGACAGACT TCTTGTGGCA
    CTACATTGTG GCAGCTATGG TTACACAATG GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96713
    Clone ID Related Accession (Same CDS sequence) NM_001350554.1 , XM_017011114.2 , XM_017011114.1 , NM_001350554.2
    Accession Version NM_001350554.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2496bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-04
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HACE1 isoform e
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357315.14, AL513472.9 and AL590402.10. On May 31, 2019 this sequence version replaced NM_001350554.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.96954.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCCAATGG TTATGGCTGA TCAACACAGG TCTGTTTCTG AACTACTATC AAATTCAAAA 
    TTTGATGTCA ATTATGCATT CGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT
    TGTGGATCGG TGGAATGCTT GGTTTTGCTG TTAAAGAAAG GAGCAAATCC TAACTATCAA
    GATATTTCAG GCTGTACACC CCTTCATTTG GCAGCAAGAA ATGGGCAGAA GAAATGTATG
    AGTAAATTAT TAGAATATAG CGCTGATGTC AACATTTGTA ATAATGAAGG CCTTACAGCA
    ACAGTGCAGT GCTTGCTAGA CAGTGGTGCT GATATTAACA GGCCAAATGT ATCAGGAGCA
    ACTCCATTGT ACTTTGCTTG CAGTCATGGT CAGAGAGATA CAGCACAGAT CCTACTATTA
    CGAGGAGCCA AATATCTGCC AGATAAAAAT GGAGTAACTC CTCTGGATTT ATGTGTACAG
    GGTGGATATG GAGAGACTTG TGAAGTATTA ATTCAATATC ACCCGAGGCT TTTTCAGACT
    ATTATTCAAA TGACACAGAA TGAAGACCTC CGAGAAAACA TGTTACGGCA AGTTCTGGAG
    CATTTGTCTC AGCAAAGTGA AAGCCAGTAC CTAAAGATTC TAACAAGCCT TGCTGAAGTT
    GCTACAACAA ATGGTCATAA ACTGCTTAGC CTCTCTAGCA ATTATGATGC TCAAATGAAG
    AGCCTTTTAA GGATTGTGAG AATGTTTTGT CACGTCTTTC GAATTGGTCC ATCCTCCCCC
    AGTAATGGAA TTGATATGGG CTACAATGGG AATAAAACTC CAAGAAGCCA GGTGTTCAAG
    CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT GAATGGCTAG TTTTAATAGC CACAGAATTG
    ATGAAAAACA AAAGAGACTC AACAGAGATC ACTTCTATTT TACTGAAACA AAAAGGCCAA
    GATCAAGATG CTGCTTCCAT TCCTCCATTT GAACCTCCAG GACCTGGGAG CTATGAAAAT
    CTGTCCACTG GCACAAGGGA ATCTAAACCA GATGCTCTTG CAGGGAGACA GGAAGCCAGT
    GCAGATTGTC AGGATGTTAT TTCTATGACA GCTAACCGGC TAAGTGCTGT CATTCAAGCT
    TTTTACATGT GCTGTTCTTG TCAGATGCCT CCGGGAATGA CTTCACCTCG TTTCATTGAA
    TTTGTCTGCA AACATGATGA AGTTTTAAAA TGCTTTGTTA ATAGAAATCC CAAAATTATA
    TTTGACCACT TTCACTTTCT CCTTGAATGT CCTGAGTTGA TGTCAAGATT CATGCATATC
    ATAAAAGCAC AGCCTTTTAA AGATCGCTGT GAATGGTTCT ATGAACATTT GCATTCAGGA
    CAGCCAGATT CAGATATGGT GCACAGGCCA GTGAATGAAA ATGATATCCT GCTGGTTCAC
    AGAGATTCTA TTTTTAGGAG TAGCTGTGAA GTTGTGTCAA AAGCAAATTG TGCAAAGCTA
    AAGCAAGGGA TTGCTGTACG GTTCCATGGA GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT
    GAGTGGTTTG ATATTCTGTC CAATGAGATA GTCAATCCTG ATTATGCATT GTTTACCCAG
    TCAGCTGATG GAACAACTTT TCAGCCTAAT AGCAACTCTT ATGTAAATCC TGATCACTTG
    AACTATTTTC GGTTTGCTGG GCAGATCTTG GGATTAGCGT TGAACCACAG GCAGCTGGTC
    AATATTTACT TCACACGATC CTTCTACAAG CACATTCTTG GTATTCCTGT AAATTACCAA
    GATGTGGCAT CCATTGATCC AGAATATGCG AAAAATTTGC AATGGATTTT AGATAATGAT
    ATAAGTGATC TGGGTCTAGA ACTAACTTTT TCTGTTGAGA CTGATGTGTT TGGAGCAATG
    GAAGAGGTGC CTTTGAAACC TGGGGGTGGG AGTATTCTTG TGACACAAAA TAATAAAGCG
    GAGTACGTCC AGCTTGTTAC TGAACTTCGA ATGACAAGAG CCATTCAGCC TCAGATCAAT
    GCTTTTTTAC AGGGCTTTCA TATGTTCATT CCACCCTCCC TCATACAGCT TTTTGATGAA
    TATGAATTGG AGCTACTGCT TTCTGGCATG CCAGAAATTG ATGTGAGTGA TTGGATAAAA
    AATACAGAAT ACACAAGTGG CTATGAAAGA GAAGATCCAG TTATTCAGTG GTTCTGGGAA
    GTTGTAGAAG ACATTACTCA AGAGGAGAGA GTTCTTCTCT TACAGTTTGT TACGGGCAGT
    TCCAGGGTCC CACATGGTGG GTTTGCTAAT ATCATGGGTG GAAGTGGATT GCAAAACTTT
    ACAATCGCTG CTGTGCCATA TACTCCAAAT CTTTTACCAA CTTCAAGCAC ATGCATCAAC
    ATGCTCAAGT TACCTGAATA CCCAAGTAAA GAAATACTCA AGGACAGACT TCTTGTGGCA
    CTACATTGTG GCAGCTATGG TTACACAATG GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337483.1
    CDS438..2933
    Translation

    Target ORF information:

    RefSeq Version NM_001350554.2
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001350554.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCCAATGG TTATGGCTGA TCAACACAGG TCTGTTTCTG AACTACTATC AAATTCAAAA 
    TTTGATGTCA ATTATGCATT CGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT
    TGTGGATCGG TGGAATGCTT GGTTTTGCTG TTAAAGAAAG GAGCAAATCC TAACTATCAA
    GATATTTCAG GCTGTACACC CCTTCATTTG GCAGCAAGAA ATGGGCAGAA GAAATGTATG
    AGTAAATTAT TAGAATATAG CGCTGATGTC AACATTTGTA ATAATGAAGG CCTTACAGCA
    ACAGTGCAGT GCTTGCTAGA CAGTGGTGCT GATATTAACA GGCCAAATGT ATCAGGAGCA
    ACTCCATTGT ACTTTGCTTG CAGTCATGGT CAGAGAGATA CAGCACAGAT CCTACTATTA
    CGAGGAGCCA AATATCTGCC AGATAAAAAT GGAGTAACTC CTCTGGATTT ATGTGTACAG
    GGTGGATATG GAGAGACTTG TGAAGTATTA ATTCAATATC ACCCGAGGCT TTTTCAGACT
    ATTATTCAAA TGACACAGAA TGAAGACCTC CGAGAAAACA TGTTACGGCA AGTTCTGGAG
    CATTTGTCTC AGCAAAGTGA AAGCCAGTAC CTAAAGATTC TAACAAGCCT TGCTGAAGTT
    GCTACAACAA ATGGTCATAA ACTGCTTAGC CTCTCTAGCA ATTATGATGC TCAAATGAAG
    AGCCTTTTAA GGATTGTGAG AATGTTTTGT CACGTCTTTC GAATTGGTCC ATCCTCCCCC
    AGTAATGGAA TTGATATGGG CTACAATGGG AATAAAACTC CAAGAAGCCA GGTGTTCAAG
    CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT GAATGGCTAG TTTTAATAGC CACAGAATTG
    ATGAAAAACA AAAGAGACTC AACAGAGATC ACTTCTATTT TACTGAAACA AAAAGGCCAA
    GATCAAGATG CTGCTTCCAT TCCTCCATTT GAACCTCCAG GACCTGGGAG CTATGAAAAT
    CTGTCCACTG GCACAAGGGA ATCTAAACCA GATGCTCTTG CAGGGAGACA GGAAGCCAGT
    GCAGATTGTC AGGATGTTAT TTCTATGACA GCTAACCGGC TAAGTGCTGT CATTCAAGCT
    TTTTACATGT GCTGTTCTTG TCAGATGCCT CCGGGAATGA CTTCACCTCG TTTCATTGAA
    TTTGTCTGCA AACATGATGA AGTTTTAAAA TGCTTTGTTA ATAGAAATCC CAAAATTATA
    TTTGACCACT TTCACTTTCT CCTTGAATGT CCTGAGTTGA TGTCAAGATT CATGCATATC
    ATAAAAGCAC AGCCTTTTAA AGATCGCTGT GAATGGTTCT ATGAACATTT GCATTCAGGA
    CAGCCAGATT CAGATATGGT GCACAGGCCA GTGAATGAAA ATGATATCCT GCTGGTTCAC
    AGAGATTCTA TTTTTAGGAG TAGCTGTGAA GTTGTGTCAA AAGCAAATTG TGCAAAGCTA
    AAGCAAGGGA TTGCTGTACG GTTCCATGGA GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT
    GAGTGGTTTG ATATTCTGTC CAATGAGATA GTCAATCCTG ATTATGCATT GTTTACCCAG
    TCAGCTGATG GAACAACTTT TCAGCCTAAT AGCAACTCTT ATGTAAATCC TGATCACTTG
    AACTATTTTC GGTTTGCTGG GCAGATCTTG GGATTAGCGT TGAACCACAG GCAGCTGGTC
    AATATTTACT TCACACGATC CTTCTACAAG CACATTCTTG GTATTCCTGT AAATTACCAA
    GATGTGGCAT CCATTGATCC AGAATATGCG AAAAATTTGC AATGGATTTT AGATAATGAT
    ATAAGTGATC TGGGTCTAGA ACTAACTTTT TCTGTTGAGA CTGATGTGTT TGGAGCAATG
    GAAGAGGTGC CTTTGAAACC TGGGGGTGGG AGTATTCTTG TGACACAAAA TAATAAAGCG
    GAGTACGTCC AGCTTGTTAC TGAACTTCGA ATGACAAGAG CCATTCAGCC TCAGATCAAT
    GCTTTTTTAC AGGGCTTTCA TATGTTCATT CCACCCTCCC TCATACAGCT TTTTGATGAA
    TATGAATTGG AGCTACTGCT TTCTGGCATG CCAGAAATTG ATGTGAGTGA TTGGATAAAA
    AATACAGAAT ACACAAGTGG CTATGAAAGA GAAGATCCAG TTATTCAGTG GTTCTGGGAA
    GTTGTAGAAG ACATTACTCA AGAGGAGAGA GTTCTTCTCT TACAGTTTGT TACGGGCAGT
    TCCAGGGTCC CACATGGTGG GTTTGCTAAT ATCATGGGTG GAAGTGGATT GCAAAACTTT
    ACAATCGCTG CTGTGCCATA TACTCCAAAT CTTTTACCAA CTTCAAGCAC ATGCATCAAC
    ATGCTCAAGT TACCTGAATA CCCAAGTAAA GAAATACTCA AGGACAGACT TCTTGTGGCA
    CTACATTGTG GCAGCTATGG TTACACAATG GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96713
    Clone ID Related Accession (Same CDS sequence) NM_001350554.1 , XM_017011114.2 , XM_017011114.1 , NM_001350554.2
    Accession Version XM_017011114.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2496bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HACE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017011114.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCCAATGG TTATGGCTGA TCAACACAGG TCTGTTTCTG AACTACTATC AAATTCAAAA 
    TTTGATGTCA ATTATGCATT CGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT
    TGTGGATCGG TGGAATGCTT GGTTTTGCTG TTAAAGAAAG GAGCAAATCC TAACTATCAA
    GATATTTCAG GCTGTACACC CCTTCATTTG GCAGCAAGAA ATGGGCAGAA GAAATGTATG
    AGTAAATTAT TAGAATATAG CGCTGATGTC AACATTTGTA ATAATGAAGG CCTTACAGCA
    ACAGTGCAGT GCTTGCTAGA CAGTGGTGCT GATATTAACA GGCCAAATGT ATCAGGAGCA
    ACTCCATTGT ACTTTGCTTG CAGTCATGGT CAGAGAGATA CAGCACAGAT CCTACTATTA
    CGAGGAGCCA AATATCTGCC AGATAAAAAT GGAGTAACTC CTCTGGATTT ATGTGTACAG
    GGTGGATATG GAGAGACTTG TGAAGTATTA ATTCAATATC ACCCGAGGCT TTTTCAGACT
    ATTATTCAAA TGACACAGAA TGAAGACCTC CGAGAAAACA TGTTACGGCA AGTTCTGGAG
    CATTTGTCTC AGCAAAGTGA AAGCCAGTAC CTAAAGATTC TAACAAGCCT TGCTGAAGTT
    GCTACAACAA ATGGTCATAA ACTGCTTAGC CTCTCTAGCA ATTATGATGC TCAAATGAAG
    AGCCTTTTAA GGATTGTGAG AATGTTTTGT CACGTCTTTC GAATTGGTCC ATCCTCCCCC
    AGTAATGGAA TTGATATGGG CTACAATGGG AATAAAACTC CAAGAAGCCA GGTGTTCAAG
    CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT GAATGGCTAG TTTTAATAGC CACAGAATTG
    ATGAAAAACA AAAGAGACTC AACAGAGATC ACTTCTATTT TACTGAAACA AAAAGGCCAA
    GATCAAGATG CTGCTTCCAT TCCTCCATTT GAACCTCCAG GACCTGGGAG CTATGAAAAT
    CTGTCCACTG GCACAAGGGA ATCTAAACCA GATGCTCTTG CAGGGAGACA GGAAGCCAGT
    GCAGATTGTC AGGATGTTAT TTCTATGACA GCTAACCGGC TAAGTGCTGT CATTCAAGCT
    TTTTACATGT GCTGTTCTTG TCAGATGCCT CCGGGAATGA CTTCACCTCG TTTCATTGAA
    TTTGTCTGCA AACATGATGA AGTTTTAAAA TGCTTTGTTA ATAGAAATCC CAAAATTATA
    TTTGACCACT TTCACTTTCT CCTTGAATGT CCTGAGTTGA TGTCAAGATT CATGCATATC
    ATAAAAGCAC AGCCTTTTAA AGATCGCTGT GAATGGTTCT ATGAACATTT GCATTCAGGA
    CAGCCAGATT CAGATATGGT GCACAGGCCA GTGAATGAAA ATGATATCCT GCTGGTTCAC
    AGAGATTCTA TTTTTAGGAG TAGCTGTGAA GTTGTGTCAA AAGCAAATTG TGCAAAGCTA
    AAGCAAGGGA TTGCTGTACG GTTCCATGGA GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT
    GAGTGGTTTG ATATTCTGTC CAATGAGATA GTCAATCCTG ATTATGCATT GTTTACCCAG
    TCAGCTGATG GAACAACTTT TCAGCCTAAT AGCAACTCTT ATGTAAATCC TGATCACTTG
    AACTATTTTC GGTTTGCTGG GCAGATCTTG GGATTAGCGT TGAACCACAG GCAGCTGGTC
    AATATTTACT TCACACGATC CTTCTACAAG CACATTCTTG GTATTCCTGT AAATTACCAA
    GATGTGGCAT CCATTGATCC AGAATATGCG AAAAATTTGC AATGGATTTT AGATAATGAT
    ATAAGTGATC TGGGTCTAGA ACTAACTTTT TCTGTTGAGA CTGATGTGTT TGGAGCAATG
    GAAGAGGTGC CTTTGAAACC TGGGGGTGGG AGTATTCTTG TGACACAAAA TAATAAAGCG
    GAGTACGTCC AGCTTGTTAC TGAACTTCGA ATGACAAGAG CCATTCAGCC TCAGATCAAT
    GCTTTTTTAC AGGGCTTTCA TATGTTCATT CCACCCTCCC TCATACAGCT TTTTGATGAA
    TATGAATTGG AGCTACTGCT TTCTGGCATG CCAGAAATTG ATGTGAGTGA TTGGATAAAA
    AATACAGAAT ACACAAGTGG CTATGAAAGA GAAGATCCAG TTATTCAGTG GTTCTGGGAA
    GTTGTAGAAG ACATTACTCA AGAGGAGAGA GTTCTTCTCT TACAGTTTGT TACGGGCAGT
    TCCAGGGTCC CACATGGTGG GTTTGCTAAT ATCATGGGTG GAAGTGGATT GCAAAACTTT
    ACAATCGCTG CTGTGCCATA TACTCCAAAT CTTTTACCAA CTTCAAGCAC ATGCATCAAC
    ATGCTCAAGT TACCTGAATA CCCAAGTAAA GAAATACTCA AGGACAGACT TCTTGTGGCA
    CTACATTGTG GCAGCTATGG TTACACAATG GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016866603.1
    CDS529..3024
    Translation

    Target ORF information:

    RefSeq Version XM_017011114.2
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017011114.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCCAATGG TTATGGCTGA TCAACACAGG TCTGTTTCTG AACTACTATC AAATTCAAAA 
    TTTGATGTCA ATTATGCATT CGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT
    TGTGGATCGG TGGAATGCTT GGTTTTGCTG TTAAAGAAAG GAGCAAATCC TAACTATCAA
    GATATTTCAG GCTGTACACC CCTTCATTTG GCAGCAAGAA ATGGGCAGAA GAAATGTATG
    AGTAAATTAT TAGAATATAG CGCTGATGTC AACATTTGTA ATAATGAAGG CCTTACAGCA
    ACAGTGCAGT GCTTGCTAGA CAGTGGTGCT GATATTAACA GGCCAAATGT ATCAGGAGCA
    ACTCCATTGT ACTTTGCTTG CAGTCATGGT CAGAGAGATA CAGCACAGAT CCTACTATTA
    CGAGGAGCCA AATATCTGCC AGATAAAAAT GGAGTAACTC CTCTGGATTT ATGTGTACAG
    GGTGGATATG GAGAGACTTG TGAAGTATTA ATTCAATATC ACCCGAGGCT TTTTCAGACT
    ATTATTCAAA TGACACAGAA TGAAGACCTC CGAGAAAACA TGTTACGGCA AGTTCTGGAG
    CATTTGTCTC AGCAAAGTGA AAGCCAGTAC CTAAAGATTC TAACAAGCCT TGCTGAAGTT
    GCTACAACAA ATGGTCATAA ACTGCTTAGC CTCTCTAGCA ATTATGATGC TCAAATGAAG
    AGCCTTTTAA GGATTGTGAG AATGTTTTGT CACGTCTTTC GAATTGGTCC ATCCTCCCCC
    AGTAATGGAA TTGATATGGG CTACAATGGG AATAAAACTC CAAGAAGCCA GGTGTTCAAG
    CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT GAATGGCTAG TTTTAATAGC CACAGAATTG
    ATGAAAAACA AAAGAGACTC AACAGAGATC ACTTCTATTT TACTGAAACA AAAAGGCCAA
    GATCAAGATG CTGCTTCCAT TCCTCCATTT GAACCTCCAG GACCTGGGAG CTATGAAAAT
    CTGTCCACTG GCACAAGGGA ATCTAAACCA GATGCTCTTG CAGGGAGACA GGAAGCCAGT
    GCAGATTGTC AGGATGTTAT TTCTATGACA GCTAACCGGC TAAGTGCTGT CATTCAAGCT
    TTTTACATGT GCTGTTCTTG TCAGATGCCT CCGGGAATGA CTTCACCTCG TTTCATTGAA
    TTTGTCTGCA AACATGATGA AGTTTTAAAA TGCTTTGTTA ATAGAAATCC CAAAATTATA
    TTTGACCACT TTCACTTTCT CCTTGAATGT CCTGAGTTGA TGTCAAGATT CATGCATATC
    ATAAAAGCAC AGCCTTTTAA AGATCGCTGT GAATGGTTCT ATGAACATTT GCATTCAGGA
    CAGCCAGATT CAGATATGGT GCACAGGCCA GTGAATGAAA ATGATATCCT GCTGGTTCAC
    AGAGATTCTA TTTTTAGGAG TAGCTGTGAA GTTGTGTCAA AAGCAAATTG TGCAAAGCTA
    AAGCAAGGGA TTGCTGTACG GTTCCATGGA GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT
    GAGTGGTTTG ATATTCTGTC CAATGAGATA GTCAATCCTG ATTATGCATT GTTTACCCAG
    TCAGCTGATG GAACAACTTT TCAGCCTAAT AGCAACTCTT ATGTAAATCC TGATCACTTG
    AACTATTTTC GGTTTGCTGG GCAGATCTTG GGATTAGCGT TGAACCACAG GCAGCTGGTC
    AATATTTACT TCACACGATC CTTCTACAAG CACATTCTTG GTATTCCTGT AAATTACCAA
    GATGTGGCAT CCATTGATCC AGAATATGCG AAAAATTTGC AATGGATTTT AGATAATGAT
    ATAAGTGATC TGGGTCTAGA ACTAACTTTT TCTGTTGAGA CTGATGTGTT TGGAGCAATG
    GAAGAGGTGC CTTTGAAACC TGGGGGTGGG AGTATTCTTG TGACACAAAA TAATAAAGCG
    GAGTACGTCC AGCTTGTTAC TGAACTTCGA ATGACAAGAG CCATTCAGCC TCAGATCAAT
    GCTTTTTTAC AGGGCTTTCA TATGTTCATT CCACCCTCCC TCATACAGCT TTTTGATGAA
    TATGAATTGG AGCTACTGCT TTCTGGCATG CCAGAAATTG ATGTGAGTGA TTGGATAAAA
    AATACAGAAT ACACAAGTGG CTATGAAAGA GAAGATCCAG TTATTCAGTG GTTCTGGGAA
    GTTGTAGAAG ACATTACTCA AGAGGAGAGA GTTCTTCTCT TACAGTTTGT TACGGGCAGT
    TCCAGGGTCC CACATGGTGG GTTTGCTAAT ATCATGGGTG GAAGTGGATT GCAAAACTTT
    ACAATCGCTG CTGTGCCATA TACTCCAAAT CTTTTACCAA CTTCAAGCAC ATGCATCAAC
    ATGCTCAAGT TACCTGAATA CCCAAGTAAA GAAATACTCA AGGACAGACT TCTTGTGGCA
    CTACATTGTG GCAGCTATGG TTACACAATG GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96713
    Clone ID Related Accession (Same CDS sequence) NM_001350554.1 , XM_017011114.2 , XM_017011114.1 , NM_001350554.2
    Accession Version XM_017011114.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2496bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase HACE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCCAATGG TTATGGCTGA TCAACACAGG TCTGTTTCTG AACTACTATC AAATTCAAAA 
    TTTGATGTCA ATTATGCATT CGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT
    TGTGGATCGG TGGAATGCTT GGTTTTGCTG TTAAAGAAAG GAGCAAATCC TAACTATCAA
    GATATTTCAG GCTGTACACC CCTTCATTTG GCAGCAAGAA ATGGGCAGAA GAAATGTATG
    AGTAAATTAT TAGAATATAG CGCTGATGTC AACATTTGTA ATAATGAAGG CCTTACAGCA
    ACAGTGCAGT GCTTGCTAGA CAGTGGTGCT GATATTAACA GGCCAAATGT ATCAGGAGCA
    ACTCCATTGT ACTTTGCTTG CAGTCATGGT CAGAGAGATA CAGCACAGAT CCTACTATTA
    CGAGGAGCCA AATATCTGCC AGATAAAAAT GGAGTAACTC CTCTGGATTT ATGTGTACAG
    GGTGGATATG GAGAGACTTG TGAAGTATTA ATTCAATATC ACCCGAGGCT TTTTCAGACT
    ATTATTCAAA TGACACAGAA TGAAGACCTC CGAGAAAACA TGTTACGGCA AGTTCTGGAG
    CATTTGTCTC AGCAAAGTGA AAGCCAGTAC CTAAAGATTC TAACAAGCCT TGCTGAAGTT
    GCTACAACAA ATGGTCATAA ACTGCTTAGC CTCTCTAGCA ATTATGATGC TCAAATGAAG
    AGCCTTTTAA GGATTGTGAG AATGTTTTGT CACGTCTTTC GAATTGGTCC ATCCTCCCCC
    AGTAATGGAA TTGATATGGG CTACAATGGG AATAAAACTC CAAGAAGCCA GGTGTTCAAG
    CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT GAATGGCTAG TTTTAATAGC CACAGAATTG
    ATGAAAAACA AAAGAGACTC AACAGAGATC ACTTCTATTT TACTGAAACA AAAAGGCCAA
    GATCAAGATG CTGCTTCCAT TCCTCCATTT GAACCTCCAG GACCTGGGAG CTATGAAAAT
    CTGTCCACTG GCACAAGGGA ATCTAAACCA GATGCTCTTG CAGGGAGACA GGAAGCCAGT
    GCAGATTGTC AGGATGTTAT TTCTATGACA GCTAACCGGC TAAGTGCTGT CATTCAAGCT
    TTTTACATGT GCTGTTCTTG TCAGATGCCT CCGGGAATGA CTTCACCTCG TTTCATTGAA
    TTTGTCTGCA AACATGATGA AGTTTTAAAA TGCTTTGTTA ATAGAAATCC CAAAATTATA
    TTTGACCACT TTCACTTTCT CCTTGAATGT CCTGAGTTGA TGTCAAGATT CATGCATATC
    ATAAAAGCAC AGCCTTTTAA AGATCGCTGT GAATGGTTCT ATGAACATTT GCATTCAGGA
    CAGCCAGATT CAGATATGGT GCACAGGCCA GTGAATGAAA ATGATATCCT GCTGGTTCAC
    AGAGATTCTA TTTTTAGGAG TAGCTGTGAA GTTGTGTCAA AAGCAAATTG TGCAAAGCTA
    AAGCAAGGGA TTGCTGTACG GTTCCATGGA GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT
    GAGTGGTTTG ATATTCTGTC CAATGAGATA GTCAATCCTG ATTATGCATT GTTTACCCAG
    TCAGCTGATG GAACAACTTT TCAGCCTAAT AGCAACTCTT ATGTAAATCC TGATCACTTG
    AACTATTTTC GGTTTGCTGG GCAGATCTTG GGATTAGCGT TGAACCACAG GCAGCTGGTC
    AATATTTACT TCACACGATC CTTCTACAAG CACATTCTTG GTATTCCTGT AAATTACCAA
    GATGTGGCAT CCATTGATCC AGAATATGCG AAAAATTTGC AATGGATTTT AGATAATGAT
    ATAAGTGATC TGGGTCTAGA ACTAACTTTT TCTGTTGAGA CTGATGTGTT TGGAGCAATG
    GAAGAGGTGC CTTTGAAACC TGGGGGTGGG AGTATTCTTG TGACACAAAA TAATAAAGCG
    GAGTACGTCC AGCTTGTTAC TGAACTTCGA ATGACAAGAG CCATTCAGCC TCAGATCAAT
    GCTTTTTTAC AGGGCTTTCA TATGTTCATT CCACCCTCCC TCATACAGCT TTTTGATGAA
    TATGAATTGG AGCTACTGCT TTCTGGCATG CCAGAAATTG ATGTGAGTGA TTGGATAAAA
    AATACAGAAT ACACAAGTGG CTATGAAAGA GAAGATCCAG TTATTCAGTG GTTCTGGGAA
    GTTGTAGAAG ACATTACTCA AGAGGAGAGA GTTCTTCTCT TACAGTTTGT TACGGGCAGT
    TCCAGGGTCC CACATGGTGG GTTTGCTAAT ATCATGGGTG GAAGTGGATT GCAAAACTTT
    ACAATCGCTG CTGTGCCATA TACTCCAAAT CTTTTACCAA CTTCAAGCAC ATGCATCAAC
    ATGCTCAAGT TACCTGAATA CCCAAGTAAA GAAATACTCA AGGACAGACT TCTTGTGGCA
    CTACATTGTG GCAGCTATGG TTACACAATG GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016866603.1
    CDS198..2693
    Translation

    Target ORF information:

    RefSeq Version XM_017011114.1
    Organism Homo sapiens(human)
    Definition Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017011114.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCCAATGG TTATGGCTGA TCAACACAGG TCTGTTTCTG AACTACTATC AAATTCAAAA 
    TTTGATGTCA ATTATGCATT CGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT
    TGTGGATCGG TGGAATGCTT GGTTTTGCTG TTAAAGAAAG GAGCAAATCC TAACTATCAA
    GATATTTCAG GCTGTACACC CCTTCATTTG GCAGCAAGAA ATGGGCAGAA GAAATGTATG
    AGTAAATTAT TAGAATATAG CGCTGATGTC AACATTTGTA ATAATGAAGG CCTTACAGCA
    ACAGTGCAGT GCTTGCTAGA CAGTGGTGCT GATATTAACA GGCCAAATGT ATCAGGAGCA
    ACTCCATTGT ACTTTGCTTG CAGTCATGGT CAGAGAGATA CAGCACAGAT CCTACTATTA
    CGAGGAGCCA AATATCTGCC AGATAAAAAT GGAGTAACTC CTCTGGATTT ATGTGTACAG
    GGTGGATATG GAGAGACTTG TGAAGTATTA ATTCAATATC ACCCGAGGCT TTTTCAGACT
    ATTATTCAAA TGACACAGAA TGAAGACCTC CGAGAAAACA TGTTACGGCA AGTTCTGGAG
    CATTTGTCTC AGCAAAGTGA AAGCCAGTAC CTAAAGATTC TAACAAGCCT TGCTGAAGTT
    GCTACAACAA ATGGTCATAA ACTGCTTAGC CTCTCTAGCA ATTATGATGC TCAAATGAAG
    AGCCTTTTAA GGATTGTGAG AATGTTTTGT CACGTCTTTC GAATTGGTCC ATCCTCCCCC
    AGTAATGGAA TTGATATGGG CTACAATGGG AATAAAACTC CAAGAAGCCA GGTGTTCAAG
    CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT GAATGGCTAG TTTTAATAGC CACAGAATTG
    ATGAAAAACA AAAGAGACTC AACAGAGATC ACTTCTATTT TACTGAAACA AAAAGGCCAA
    GATCAAGATG CTGCTTCCAT TCCTCCATTT GAACCTCCAG GACCTGGGAG CTATGAAAAT
    CTGTCCACTG GCACAAGGGA ATCTAAACCA GATGCTCTTG CAGGGAGACA GGAAGCCAGT
    GCAGATTGTC AGGATGTTAT TTCTATGACA GCTAACCGGC TAAGTGCTGT CATTCAAGCT
    TTTTACATGT GCTGTTCTTG TCAGATGCCT CCGGGAATGA CTTCACCTCG TTTCATTGAA
    TTTGTCTGCA AACATGATGA AGTTTTAAAA TGCTTTGTTA ATAGAAATCC CAAAATTATA
    TTTGACCACT TTCACTTTCT CCTTGAATGT CCTGAGTTGA TGTCAAGATT CATGCATATC
    ATAAAAGCAC AGCCTTTTAA AGATCGCTGT GAATGGTTCT ATGAACATTT GCATTCAGGA
    CAGCCAGATT CAGATATGGT GCACAGGCCA GTGAATGAAA ATGATATCCT GCTGGTTCAC
    AGAGATTCTA TTTTTAGGAG TAGCTGTGAA GTTGTGTCAA AAGCAAATTG TGCAAAGCTA
    AAGCAAGGGA TTGCTGTACG GTTCCATGGA GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT
    GAGTGGTTTG ATATTCTGTC CAATGAGATA GTCAATCCTG ATTATGCATT GTTTACCCAG
    TCAGCTGATG GAACAACTTT TCAGCCTAAT AGCAACTCTT ATGTAAATCC TGATCACTTG
    AACTATTTTC GGTTTGCTGG GCAGATCTTG GGATTAGCGT TGAACCACAG GCAGCTGGTC
    AATATTTACT TCACACGATC CTTCTACAAG CACATTCTTG GTATTCCTGT AAATTACCAA
    GATGTGGCAT CCATTGATCC AGAATATGCG AAAAATTTGC AATGGATTTT AGATAATGAT
    ATAAGTGATC TGGGTCTAGA ACTAACTTTT TCTGTTGAGA CTGATGTGTT TGGAGCAATG
    GAAGAGGTGC CTTTGAAACC TGGGGGTGGG AGTATTCTTG TGACACAAAA TAATAAAGCG
    GAGTACGTCC AGCTTGTTAC TGAACTTCGA ATGACAAGAG CCATTCAGCC TCAGATCAAT
    GCTTTTTTAC AGGGCTTTCA TATGTTCATT CCACCCTCCC TCATACAGCT TTTTGATGAA
    TATGAATTGG AGCTACTGCT TTCTGGCATG CCAGAAATTG ATGTGAGTGA TTGGATAAAA
    AATACAGAAT ACACAAGTGG CTATGAAAGA GAAGATCCAG TTATTCAGTG GTTCTGGGAA
    GTTGTAGAAG ACATTACTCA AGAGGAGAGA GTTCTTCTCT TACAGTTTGT TACGGGCAGT
    TCCAGGGTCC CACATGGTGG GTTTGCTAAT ATCATGGGTG GAAGTGGATT GCAAAACTTT
    ACAATCGCTG CTGTGCCATA TACTCCAAAT CTTTTACCAA CTTCAAGCAC ATGCATCAAC
    ATGCTCAAGT TACCTGAATA CCCAAGTAAA GAAATACTCA AGGACAGACT TCTTGTGGCA
    CTACATTGTG GCAGCTATGG TTACACAATG GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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