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RAG1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol RAG1
Entrez Gene ID 5896
Full Name recombination activating gene 1
Synonyms RAG-1, RNF74
General protein information
Preferred Names
V(D)J recombination-activating protein 1
Names
V(D)J recombination-activating protein 1
RING finger protein 74
recombination activating protein 1
NP_000439.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11p13

Summary The protein encoded by this gene is involved in activation of immunoglobulin V-D-J recombination. The encoded protein is involved in recognition of the DNA substrate, but stable binding and cleavage activity also requires RAG2. Defects in this gene can be the cause of several diseases. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

179615

Disorder Html: Severe combined immunodeficiency, B cell-negative, 601457 (3);

mRNA and Protein(s)

mRNA Protein Name
XM_011520250 XP_011518552 V(D)J recombination-activating protein 1 isoform X1
XM_011520251 XP_011518553 V(D)J recombination-activating protein 1 isoform X1
XM_005253041 XP_005253098 V(D)J recombination-activating protein 1 isoform X1
NM_000448 NP_000439 V(D)J recombination-activating protein 1



Homo sapiens (human) RAG1 NP_000439.1
Pan troglodytes (chimpanzee) RAG1 XP_001154240.1
Macaca mulatta (Rhesus monkey) RAG1 XP_001084631.1
Canis lupus familiaris (dog) RAG1 XP_540538.1
Bos taurus (cattle) RAG1 XP_005216456.1
Mus musculus (house mouse) Rag1 NP_033045.2
Rattus norvegicus (Norway rat) Rag1 NP_445920.1
Gallus gallus (chicken) RAG1 NP_001026359.1
Danio rerio (zebrafish) rag1 NP_571464.1
Xenopus (Silurana) tropicalis (western clawed frog) rag1 XP_002937338.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RAG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Free ORF Clone
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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu44935 XM_011520250 PREDICTED: Homo sapiens recombination activating gene 1 (RAG1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu44935 XM_011520251 PREDICTED: Homo sapiens recombination activating gene 1 (RAG1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu44935 XM_005253041 PREDICTED: Homo sapiens recombination activating gene 1 (RAG1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu17854
NM_000448 Homo sapiens recombination activating gene 1 (RAG1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$399.50-$559.30
$799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu44935
Clone ID Related Accession (Same CDS sequence) XM_005253041 , XM_011520250 , XM_011520251
Accession Version XM_011520250.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3132bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product V(D)J recombination-activating protein 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009237.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGGCAGCCT CTTTCCCACC CACCTTGGGA CTCAGTTCTG CCCCAGATGA AATTCAGCAC 
CCACATATTA AATTTTCAGA ATGGAAATTT AAGCTGTTCC GGGTGAGATC CTTTGAAAAG
ACACCTGAAG AAGCTCAAAA GGAAAAGAAG GATTCCTTTG AGGGGAAACC CTCTCTGGAG
CAATCTCCAG CAGTCCTGGA CAAGGCTGAT GGTCAGAAGC CAGTCCCAAC TCAGCCATTG
TTAAAAGCCC ACCCTAAGTT TTCAAAGAAA TTTCACGACA ACGAGAAAGC AAGAGGCAAA
GCGATCCATC AAGCCAACCT TCGACATCTC TGCCGCATCT GTGGGAATTC TTTTAGAGCT
GATGAGCACA ACAGGAGATA TCCAGTCCAT GGTCCTGTGG ATGGTAAAAC CCTAGGCCTT
TTACGAAAGA AGGAAAAGAG AGCTACTTCC TGGCCGGACC TCATTGCCAA GGTTTTCCGG
ATCGATGTGA AGGCAGATGT TGACTCGATC CACCCCACTG AGTTCTGCCA TAACTGCTGG
AGCATCATGC ACAGGAAGTT TAGCAGTGCC CCATGTGAGG TTTACTTCCC GAGGAACGTG
ACCATGGAGT GGCACCCCCA CACACCATCC TGTGACATCT GCAACACTGC CCGTCGGGGA
CTCAAGAGGA AGAGTCTTCA GCCAAACTTG CAGCTCAGCA AAAAACTCAA AACTGTGCTT
GACCAAGCAA GACAAGCCCG TCAGCACAAG AGAAGAGCTC AGGCAAGGAT CAGCAGCAAG
GATGTCATGA AGAAGATCGC CAACTGCAGT AAGATACATC TTAGTACCAA GCTCCTTGCA
GTGGACTTCC CAGAGCACTT TGTGAAATCC ATCTCCTGCC AGATCTGTGA ACACATTCTG
GCTGACCCTG TGGAGACCAA CTGTAAGCAT GTCTTTTGCC GGGTCTGCAT TCTCAGATGC
CTCAAAGTCA TGGGCAGCTA TTGTCCCTCT TGCCGATATC CATGCTTCCC TACTGACCTG
GAGAGTCCAG TGAAGTCCTT TCTGAGCGTC TTGAATTCCC TGATGGTGAA ATGTCCAGCA
AAAGAGTGCA ATGAGGAGGT CAGTTTGGAA AAATATAATC ACCACATCTC AAGTCACAAG
GAATCAAAAG AGATTTTTGT GCACATTAAT AAAGGGGGCC GGCCCCGCCA ACATCTTCTG
TCGCTGACTC GGAGAGCTCA GAAGCACCGG CTGAGGGAGC TCAAGCTGCA AGTCAAAGCC
TTTGCTGACA AAGAAGAAGG TGGAGATGTG AAGTCCGTGT GCATGACCTT GTTCCTGCTG
GCTCTGAGGG CGAGGAATGA GCACAGGCAA GCTGATGAGC TGGAGGCCAT CATGCAGGGA
AAGGGCTCTG GCCTGCAGCC AGCTGTTTGC TTGGCCATCC GTGTCAACAC CTTCCTCAGC
TGCAGTCAGT ACCACAAGAT GTACAGGACT GTGAAAGCCA TCACAGGGAG ACAGATTTTT
CAGCCTTTGC ATGCCCTTCG GAATGCTGAG AAGGTACTTC TGCCAGGCTA CCACCACTTT
GAGTGGCAGC CACCTCTGAA GAATGTGTCT TCCAGCACTG ATGTTGGCAT TATTGATGGG
CTGTCTGGAC TATCATCCTC TGTGGATGAT TACCCAGTGG ACACCATTGC AAAGAGGTTC
CGCTATGATT CAGCTTTGGT GTCTGCTTTG ATGGACATGG AAGAAGACAT CTTGGAAGGC
ATGAGATCCC AAGACCTTGA TGATTACCTG AATGGCCCCT TCACTGTGGT GGTGAAGGAG
TCTTGTGATG GAATGGGAGA CGTGAGTGAG AAGCATGGGA GTGGGCCTGT AGTTCCAGAA
AAGGCAGTCC GTTTTTCATT CACAATCATG AAAATTACTA TTGCCCACAG CTCTCAGAAT
GTGAAAGTAT TTGAAGAAGC CAAACCTAAC TCTGAACTGT GTTGCAAGCC ATTGTGCCTT
ATGCTGGCAG ATGAGTCTGA CCACGAGACG CTGACTGCCA TCCTGAGTCC TCTCATTGCT
GAGAGGGAGG CCATGAAGAG CAGTGAATTA ATGCTTGAGC TGGGAGGCAT TCTCCGGACT
TTCAAGTTCA TCTTCAGGGG CACCGGCTAT GATGAAAAAC TTGTGCGGGA AGTGGAAGGC
CTCGAGGCTT CTGGCTCAGT CTACATTTGT ACTCTTTGTG ATGCCACCCG TCTGGAAGCC
TCTCAAAATC TTGTCTTCCA CTCTATAACC AGAAGCCATG CTGAGAACCT GGAACGTTAT
GAGGTCTGGC GTTCCAACCC TTACCATGAG TCTGTGGAAG AACTGCGGGA TCGGGTGAAA
GGGGTCTCAG CTAAACCTTT CATTGAGACA GTCCCTTCCA TAGATGCACT CCACTGTGAC
ATTGGCAATG CAGCTGAGTT CTACAAGATC TTCCAGCTAG AGATAGGGGA AGTGTATAAG
AATCCCAATG CTTCCAAAGA GGAAAGGAAA AGGTGGCAGG CCACACTGGA CAAGCATCTC
CGGAAGAAGA TGAACCTCAA ACCAATCATG AGGATGAATG GCAACTTTGC CAGGAAGCTC
ATGACCAAAG AGACTGTGGA TGCAGTTTGT GAGTTAATTC CTTCCGAGGA GAGGCACGAG
GCTCTGAGGG AGCTGATGGA TCTTTACCTG AAGATGAAAC CAGTATGGCG ATCATCATGC
CCTGCTAAAG AGTGCCCAGA ATCCCTCTGC CAGTACAGTT TCAATTCACA GCGTTTTGCT
GAGCTCCTTT CTACGAAGTT CAAGTATAGG TATGAGGGAA AAATCACCAA TTATTTTCAC
AAAACCCTGG CCCATGTTCC TGAAATTATT GAGAGGGATG GCTCCATTGG GGCATGGGCA
AGTGAGGGAA ATGAGTCTGG TAACAAACTG TTTAGGCGCT TCCGGAAAAT GAATGCCAGG
CAGTCCAAAT GCTATGAGAT GGAAGATGTC CTGAAACACC ACTGGTTGTA CACCTCCAAA
TACCTCCAGA AGTTTATGAA TGCTCATAAT GCATTAAAAA CCTCTGGGTT TACCATGAAC
CCTCAGGCAA GCTTAGGGGA CCCATTAGGC ATAGAGGACT CTCTGGAAAG CCAAGATTCA
ATGGAATTTT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011518552.1
CDS267..3398
Misc Feature(1)1143..1271(+)
Misc Feature(2)1143..1259(+)
Misc Feature(3)1326..1415(+)
Misc Feature(4)1803..3125(+)
Translation

Target ORF information:

RefSeq Version XM_011520250
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens recombination activating gene 1 (RAG1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011520250

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGGCAGCCT CTTTCCCACC CACCTTGGGA CTCAGTTCTG CCCCAGATGA AATTCAGCAC 
CCACATATTA AATTTTCAGA ATGGAAATTT AAGCTGTTCC GGGTGAGATC CTTTGAAAAG
ACACCTGAAG AAGCTCAAAA GGAAAAGAAG GATTCCTTTG AGGGGAAACC CTCTCTGGAG
CAATCTCCAG CAGTCCTGGA CAAGGCTGAT GGTCAGAAGC CAGTCCCAAC TCAGCCATTG
TTAAAAGCCC ACCCTAAGTT TTCAAAGAAA TTTCACGACA ACGAGAAAGC AAGAGGCAAA
GCGATCCATC AAGCCAACCT TCGACATCTC TGCCGCATCT GTGGGAATTC TTTTAGAGCT
GATGAGCACA ACAGGAGATA TCCAGTCCAT GGTCCTGTGG ATGGTAAAAC CCTAGGCCTT
TTACGAAAGA AGGAAAAGAG AGCTACTTCC TGGCCGGACC TCATTGCCAA GGTTTTCCGG
ATCGATGTGA AGGCAGATGT TGACTCGATC CACCCCACTG AGTTCTGCCA TAACTGCTGG
AGCATCATGC ACAGGAAGTT TAGCAGTGCC CCATGTGAGG TTTACTTCCC GAGGAACGTG
ACCATGGAGT GGCACCCCCA CACACCATCC TGTGACATCT GCAACACTGC CCGTCGGGGA
CTCAAGAGGA AGAGTCTTCA GCCAAACTTG CAGCTCAGCA AAAAACTCAA AACTGTGCTT
GACCAAGCAA GACAAGCCCG TCAGCACAAG AGAAGAGCTC AGGCAAGGAT CAGCAGCAAG
GATGTCATGA AGAAGATCGC CAACTGCAGT AAGATACATC TTAGTACCAA GCTCCTTGCA
GTGGACTTCC CAGAGCACTT TGTGAAATCC ATCTCCTGCC AGATCTGTGA ACACATTCTG
GCTGACCCTG TGGAGACCAA CTGTAAGCAT GTCTTTTGCC GGGTCTGCAT TCTCAGATGC
CTCAAAGTCA TGGGCAGCTA TTGTCCCTCT TGCCGATATC CATGCTTCCC TACTGACCTG
GAGAGTCCAG TGAAGTCCTT TCTGAGCGTC TTGAATTCCC TGATGGTGAA ATGTCCAGCA
AAAGAGTGCA ATGAGGAGGT CAGTTTGGAA AAATATAATC ACCACATCTC AAGTCACAAG
GAATCAAAAG AGATTTTTGT GCACATTAAT AAAGGGGGCC GGCCCCGCCA ACATCTTCTG
TCGCTGACTC GGAGAGCTCA GAAGCACCGG CTGAGGGAGC TCAAGCTGCA AGTCAAAGCC
TTTGCTGACA AAGAAGAAGG TGGAGATGTG AAGTCCGTGT GCATGACCTT GTTCCTGCTG
GCTCTGAGGG CGAGGAATGA GCACAGGCAA GCTGATGAGC TGGAGGCCAT CATGCAGGGA
AAGGGCTCTG GCCTGCAGCC AGCTGTTTGC TTGGCCATCC GTGTCAACAC CTTCCTCAGC
TGCAGTCAGT ACCACAAGAT GTACAGGACT GTGAAAGCCA TCACAGGGAG ACAGATTTTT
CAGCCTTTGC ATGCCCTTCG GAATGCTGAG AAGGTACTTC TGCCAGGCTA CCACCACTTT
GAGTGGCAGC CACCTCTGAA GAATGTGTCT TCCAGCACTG ATGTTGGCAT TATTGATGGG
CTGTCTGGAC TATCATCCTC TGTGGATGAT TACCCAGTGG ACACCATTGC AAAGAGGTTC
CGCTATGATT CAGCTTTGGT GTCTGCTTTG ATGGACATGG AAGAAGACAT CTTGGAAGGC
ATGAGATCCC AAGACCTTGA TGATTACCTG AATGGCCCCT TCACTGTGGT GGTGAAGGAG
TCTTGTGATG GAATGGGAGA CGTGAGTGAG AAGCATGGGA GTGGGCCTGT AGTTCCAGAA
AAGGCAGTCC GTTTTTCATT CACAATCATG AAAATTACTA TTGCCCACAG CTCTCAGAAT
GTGAAAGTAT TTGAAGAAGC CAAACCTAAC TCTGAACTGT GTTGCAAGCC ATTGTGCCTT
ATGCTGGCAG ATGAGTCTGA CCACGAGACG CTGACTGCCA TCCTGAGTCC TCTCATTGCT
GAGAGGGAGG CCATGAAGAG CAGTGAATTA ATGCTTGAGC TGGGAGGCAT TCTCCGGACT
TTCAAGTTCA TCTTCAGGGG CACCGGCTAT GATGAAAAAC TTGTGCGGGA AGTGGAAGGC
CTCGAGGCTT CTGGCTCAGT CTACATTTGT ACTCTTTGTG ATGCCACCCG TCTGGAAGCC
TCTCAAAATC TTGTCTTCCA CTCTATAACC AGAAGCCATG CTGAGAACCT GGAACGTTAT
GAGGTCTGGC GTTCCAACCC TTACCATGAG TCTGTGGAAG AACTGCGGGA TCGGGTGAAA
GGGGTCTCAG CTAAACCTTT CATTGAGACA GTCCCTTCCA TAGATGCACT CCACTGTGAC
ATTGGCAATG CAGCTGAGTT CTACAAGATC TTCCAGCTAG AGATAGGGGA AGTGTATAAG
AATCCCAATG CTTCCAAAGA GGAAAGGAAA AGGTGGCAGG CCACACTGGA CAAGCATCTC
CGGAAGAAGA TGAACCTCAA ACCAATCATG AGGATGAATG GCAACTTTGC CAGGAAGCTC
ATGACCAAAG AGACTGTGGA TGCAGTTTGT GAGTTAATTC CTTCCGAGGA GAGGCACGAG
GCTCTGAGGG AGCTGATGGA TCTTTACCTG AAGATGAAAC CAGTATGGCG ATCATCATGC
CCTGCTAAAG AGTGCCCAGA ATCCCTCTGC CAGTACAGTT TCAATTCACA GCGTTTTGCT
GAGCTCCTTT CTACGAAGTT CAAGTATAGG TATGAGGGAA AAATCACCAA TTATTTTCAC
AAAACCCTGG CCCATGTTCC TGAAATTATT GAGAGGGATG GCTCCATTGG GGCATGGGCA
AGTGAGGGAA ATGAGTCTGG TAACAAACTG TTTAGGCGCT TCCGGAAAAT GAATGCCAGG
CAGTCCAAAT GCTATGAGAT GGAAGATGTC CTGAAACACC ACTGGTTGTA CACCTCCAAA
TACCTCCAGA AGTTTATGAA TGCTCATAAT GCATTAAAAA CCTCTGGGTT TACCATGAAC
CCTCAGGCAA GCTTAGGGGA CCCATTAGGC ATAGAGGACT CTCTGGAAAG CCAAGATTCA
ATGGAATTTT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu44935
Clone ID Related Accession (Same CDS sequence) XM_005253041 , XM_011520250 , XM_011520251
Accession Version XM_011520251.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3132bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product V(D)J recombination-activating protein 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009237.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGGCAGCCT CTTTCCCACC CACCTTGGGA CTCAGTTCTG CCCCAGATGA AATTCAGCAC 
CCACATATTA AATTTTCAGA ATGGAAATTT AAGCTGTTCC GGGTGAGATC CTTTGAAAAG
ACACCTGAAG AAGCTCAAAA GGAAAAGAAG GATTCCTTTG AGGGGAAACC CTCTCTGGAG
CAATCTCCAG CAGTCCTGGA CAAGGCTGAT GGTCAGAAGC CAGTCCCAAC TCAGCCATTG
TTAAAAGCCC ACCCTAAGTT TTCAAAGAAA TTTCACGACA ACGAGAAAGC AAGAGGCAAA
GCGATCCATC AAGCCAACCT TCGACATCTC TGCCGCATCT GTGGGAATTC TTTTAGAGCT
GATGAGCACA ACAGGAGATA TCCAGTCCAT GGTCCTGTGG ATGGTAAAAC CCTAGGCCTT
TTACGAAAGA AGGAAAAGAG AGCTACTTCC TGGCCGGACC TCATTGCCAA GGTTTTCCGG
ATCGATGTGA AGGCAGATGT TGACTCGATC CACCCCACTG AGTTCTGCCA TAACTGCTGG
AGCATCATGC ACAGGAAGTT TAGCAGTGCC CCATGTGAGG TTTACTTCCC GAGGAACGTG
ACCATGGAGT GGCACCCCCA CACACCATCC TGTGACATCT GCAACACTGC CCGTCGGGGA
CTCAAGAGGA AGAGTCTTCA GCCAAACTTG CAGCTCAGCA AAAAACTCAA AACTGTGCTT
GACCAAGCAA GACAAGCCCG TCAGCACAAG AGAAGAGCTC AGGCAAGGAT CAGCAGCAAG
GATGTCATGA AGAAGATCGC CAACTGCAGT AAGATACATC TTAGTACCAA GCTCCTTGCA
GTGGACTTCC CAGAGCACTT TGTGAAATCC ATCTCCTGCC AGATCTGTGA ACACATTCTG
GCTGACCCTG TGGAGACCAA CTGTAAGCAT GTCTTTTGCC GGGTCTGCAT TCTCAGATGC
CTCAAAGTCA TGGGCAGCTA TTGTCCCTCT TGCCGATATC CATGCTTCCC TACTGACCTG
GAGAGTCCAG TGAAGTCCTT TCTGAGCGTC TTGAATTCCC TGATGGTGAA ATGTCCAGCA
AAAGAGTGCA ATGAGGAGGT CAGTTTGGAA AAATATAATC ACCACATCTC AAGTCACAAG
GAATCAAAAG AGATTTTTGT GCACATTAAT AAAGGGGGCC GGCCCCGCCA ACATCTTCTG
TCGCTGACTC GGAGAGCTCA GAAGCACCGG CTGAGGGAGC TCAAGCTGCA AGTCAAAGCC
TTTGCTGACA AAGAAGAAGG TGGAGATGTG AAGTCCGTGT GCATGACCTT GTTCCTGCTG
GCTCTGAGGG CGAGGAATGA GCACAGGCAA GCTGATGAGC TGGAGGCCAT CATGCAGGGA
AAGGGCTCTG GCCTGCAGCC AGCTGTTTGC TTGGCCATCC GTGTCAACAC CTTCCTCAGC
TGCAGTCAGT ACCACAAGAT GTACAGGACT GTGAAAGCCA TCACAGGGAG ACAGATTTTT
CAGCCTTTGC ATGCCCTTCG GAATGCTGAG AAGGTACTTC TGCCAGGCTA CCACCACTTT
GAGTGGCAGC CACCTCTGAA GAATGTGTCT TCCAGCACTG ATGTTGGCAT TATTGATGGG
CTGTCTGGAC TATCATCCTC TGTGGATGAT TACCCAGTGG ACACCATTGC AAAGAGGTTC
CGCTATGATT CAGCTTTGGT GTCTGCTTTG ATGGACATGG AAGAAGACAT CTTGGAAGGC
ATGAGATCCC AAGACCTTGA TGATTACCTG AATGGCCCCT TCACTGTGGT GGTGAAGGAG
TCTTGTGATG GAATGGGAGA CGTGAGTGAG AAGCATGGGA GTGGGCCTGT AGTTCCAGAA
AAGGCAGTCC GTTTTTCATT CACAATCATG AAAATTACTA TTGCCCACAG CTCTCAGAAT
GTGAAAGTAT TTGAAGAAGC CAAACCTAAC TCTGAACTGT GTTGCAAGCC ATTGTGCCTT
ATGCTGGCAG ATGAGTCTGA CCACGAGACG CTGACTGCCA TCCTGAGTCC TCTCATTGCT
GAGAGGGAGG CCATGAAGAG CAGTGAATTA ATGCTTGAGC TGGGAGGCAT TCTCCGGACT
TTCAAGTTCA TCTTCAGGGG CACCGGCTAT GATGAAAAAC TTGTGCGGGA AGTGGAAGGC
CTCGAGGCTT CTGGCTCAGT CTACATTTGT ACTCTTTGTG ATGCCACCCG TCTGGAAGCC
TCTCAAAATC TTGTCTTCCA CTCTATAACC AGAAGCCATG CTGAGAACCT GGAACGTTAT
GAGGTCTGGC GTTCCAACCC TTACCATGAG TCTGTGGAAG AACTGCGGGA TCGGGTGAAA
GGGGTCTCAG CTAAACCTTT CATTGAGACA GTCCCTTCCA TAGATGCACT CCACTGTGAC
ATTGGCAATG CAGCTGAGTT CTACAAGATC TTCCAGCTAG AGATAGGGGA AGTGTATAAG
AATCCCAATG CTTCCAAAGA GGAAAGGAAA AGGTGGCAGG CCACACTGGA CAAGCATCTC
CGGAAGAAGA TGAACCTCAA ACCAATCATG AGGATGAATG GCAACTTTGC CAGGAAGCTC
ATGACCAAAG AGACTGTGGA TGCAGTTTGT GAGTTAATTC CTTCCGAGGA GAGGCACGAG
GCTCTGAGGG AGCTGATGGA TCTTTACCTG AAGATGAAAC CAGTATGGCG ATCATCATGC
CCTGCTAAAG AGTGCCCAGA ATCCCTCTGC CAGTACAGTT TCAATTCACA GCGTTTTGCT
GAGCTCCTTT CTACGAAGTT CAAGTATAGG TATGAGGGAA AAATCACCAA TTATTTTCAC
AAAACCCTGG CCCATGTTCC TGAAATTATT GAGAGGGATG GCTCCATTGG GGCATGGGCA
AGTGAGGGAA ATGAGTCTGG TAACAAACTG TTTAGGCGCT TCCGGAAAAT GAATGCCAGG
CAGTCCAAAT GCTATGAGAT GGAAGATGTC CTGAAACACC ACTGGTTGTA CACCTCCAAA
TACCTCCAGA AGTTTATGAA TGCTCATAAT GCATTAAAAA CCTCTGGGTT TACCATGAAC
CCTCAGGCAA GCTTAGGGGA CCCATTAGGC ATAGAGGACT CTCTGGAAAG CCAAGATTCA
ATGGAATTTT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011518553.1
CDS365..3496
Misc Feature(1)1241..1369(+)
Misc Feature(2)1241..1357(+)
Misc Feature(3)1424..1513(+)
Misc Feature(4)1901..3223(+)
Translation

Target ORF information:

RefSeq Version XM_011520251
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens recombination activating gene 1 (RAG1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011520251

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGGCAGCCT CTTTCCCACC CACCTTGGGA CTCAGTTCTG CCCCAGATGA AATTCAGCAC 
CCACATATTA AATTTTCAGA ATGGAAATTT AAGCTGTTCC GGGTGAGATC CTTTGAAAAG
ACACCTGAAG AAGCTCAAAA GGAAAAGAAG GATTCCTTTG AGGGGAAACC CTCTCTGGAG
CAATCTCCAG CAGTCCTGGA CAAGGCTGAT GGTCAGAAGC CAGTCCCAAC TCAGCCATTG
TTAAAAGCCC ACCCTAAGTT TTCAAAGAAA TTTCACGACA ACGAGAAAGC AAGAGGCAAA
GCGATCCATC AAGCCAACCT TCGACATCTC TGCCGCATCT GTGGGAATTC TTTTAGAGCT
GATGAGCACA ACAGGAGATA TCCAGTCCAT GGTCCTGTGG ATGGTAAAAC CCTAGGCCTT
TTACGAAAGA AGGAAAAGAG AGCTACTTCC TGGCCGGACC TCATTGCCAA GGTTTTCCGG
ATCGATGTGA AGGCAGATGT TGACTCGATC CACCCCACTG AGTTCTGCCA TAACTGCTGG
AGCATCATGC ACAGGAAGTT TAGCAGTGCC CCATGTGAGG TTTACTTCCC GAGGAACGTG
ACCATGGAGT GGCACCCCCA CACACCATCC TGTGACATCT GCAACACTGC CCGTCGGGGA
CTCAAGAGGA AGAGTCTTCA GCCAAACTTG CAGCTCAGCA AAAAACTCAA AACTGTGCTT
GACCAAGCAA GACAAGCCCG TCAGCACAAG AGAAGAGCTC AGGCAAGGAT CAGCAGCAAG
GATGTCATGA AGAAGATCGC CAACTGCAGT AAGATACATC TTAGTACCAA GCTCCTTGCA
GTGGACTTCC CAGAGCACTT TGTGAAATCC ATCTCCTGCC AGATCTGTGA ACACATTCTG
GCTGACCCTG TGGAGACCAA CTGTAAGCAT GTCTTTTGCC GGGTCTGCAT TCTCAGATGC
CTCAAAGTCA TGGGCAGCTA TTGTCCCTCT TGCCGATATC CATGCTTCCC TACTGACCTG
GAGAGTCCAG TGAAGTCCTT TCTGAGCGTC TTGAATTCCC TGATGGTGAA ATGTCCAGCA
AAAGAGTGCA ATGAGGAGGT CAGTTTGGAA AAATATAATC ACCACATCTC AAGTCACAAG
GAATCAAAAG AGATTTTTGT GCACATTAAT AAAGGGGGCC GGCCCCGCCA ACATCTTCTG
TCGCTGACTC GGAGAGCTCA GAAGCACCGG CTGAGGGAGC TCAAGCTGCA AGTCAAAGCC
TTTGCTGACA AAGAAGAAGG TGGAGATGTG AAGTCCGTGT GCATGACCTT GTTCCTGCTG
GCTCTGAGGG CGAGGAATGA GCACAGGCAA GCTGATGAGC TGGAGGCCAT CATGCAGGGA
AAGGGCTCTG GCCTGCAGCC AGCTGTTTGC TTGGCCATCC GTGTCAACAC CTTCCTCAGC
TGCAGTCAGT ACCACAAGAT GTACAGGACT GTGAAAGCCA TCACAGGGAG ACAGATTTTT
CAGCCTTTGC ATGCCCTTCG GAATGCTGAG AAGGTACTTC TGCCAGGCTA CCACCACTTT
GAGTGGCAGC CACCTCTGAA GAATGTGTCT TCCAGCACTG ATGTTGGCAT TATTGATGGG
CTGTCTGGAC TATCATCCTC TGTGGATGAT TACCCAGTGG ACACCATTGC AAAGAGGTTC
CGCTATGATT CAGCTTTGGT GTCTGCTTTG ATGGACATGG AAGAAGACAT CTTGGAAGGC
ATGAGATCCC AAGACCTTGA TGATTACCTG AATGGCCCCT TCACTGTGGT GGTGAAGGAG
TCTTGTGATG GAATGGGAGA CGTGAGTGAG AAGCATGGGA GTGGGCCTGT AGTTCCAGAA
AAGGCAGTCC GTTTTTCATT CACAATCATG AAAATTACTA TTGCCCACAG CTCTCAGAAT
GTGAAAGTAT TTGAAGAAGC CAAACCTAAC TCTGAACTGT GTTGCAAGCC ATTGTGCCTT
ATGCTGGCAG ATGAGTCTGA CCACGAGACG CTGACTGCCA TCCTGAGTCC TCTCATTGCT
GAGAGGGAGG CCATGAAGAG CAGTGAATTA ATGCTTGAGC TGGGAGGCAT TCTCCGGACT
TTCAAGTTCA TCTTCAGGGG CACCGGCTAT GATGAAAAAC TTGTGCGGGA AGTGGAAGGC
CTCGAGGCTT CTGGCTCAGT CTACATTTGT ACTCTTTGTG ATGCCACCCG TCTGGAAGCC
TCTCAAAATC TTGTCTTCCA CTCTATAACC AGAAGCCATG CTGAGAACCT GGAACGTTAT
GAGGTCTGGC GTTCCAACCC TTACCATGAG TCTGTGGAAG AACTGCGGGA TCGGGTGAAA
GGGGTCTCAG CTAAACCTTT CATTGAGACA GTCCCTTCCA TAGATGCACT CCACTGTGAC
ATTGGCAATG CAGCTGAGTT CTACAAGATC TTCCAGCTAG AGATAGGGGA AGTGTATAAG
AATCCCAATG CTTCCAAAGA GGAAAGGAAA AGGTGGCAGG CCACACTGGA CAAGCATCTC
CGGAAGAAGA TGAACCTCAA ACCAATCATG AGGATGAATG GCAACTTTGC CAGGAAGCTC
ATGACCAAAG AGACTGTGGA TGCAGTTTGT GAGTTAATTC CTTCCGAGGA GAGGCACGAG
GCTCTGAGGG AGCTGATGGA TCTTTACCTG AAGATGAAAC CAGTATGGCG ATCATCATGC
CCTGCTAAAG AGTGCCCAGA ATCCCTCTGC CAGTACAGTT TCAATTCACA GCGTTTTGCT
GAGCTCCTTT CTACGAAGTT CAAGTATAGG TATGAGGGAA AAATCACCAA TTATTTTCAC
AAAACCCTGG CCCATGTTCC TGAAATTATT GAGAGGGATG GCTCCATTGG GGCATGGGCA
AGTGAGGGAA ATGAGTCTGG TAACAAACTG TTTAGGCGCT TCCGGAAAAT GAATGCCAGG
CAGTCCAAAT GCTATGAGAT GGAAGATGTC CTGAAACACC ACTGGTTGTA CACCTCCAAA
TACCTCCAGA AGTTTATGAA TGCTCATAAT GCATTAAAAA CCTCTGGGTT TACCATGAAC
CCTCAGGCAA GCTTAGGGGA CCCATTAGGC ATAGAGGACT CTCTGGAAAG CCAAGATTCA
ATGGAATTTT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu44935
Clone ID Related Accession (Same CDS sequence) XM_005253041 , XM_011520250 , XM_011520251
Accession Version XM_005253041.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3132bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product V(D)J recombination-activating protein 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009237.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578820674. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGGCAGCCT CTTTCCCACC CACCTTGGGA CTCAGTTCTG CCCCAGATGA AATTCAGCAC 
CCACATATTA AATTTTCAGA ATGGAAATTT AAGCTGTTCC GGGTGAGATC CTTTGAAAAG
ACACCTGAAG AAGCTCAAAA GGAAAAGAAG GATTCCTTTG AGGGGAAACC CTCTCTGGAG
CAATCTCCAG CAGTCCTGGA CAAGGCTGAT GGTCAGAAGC CAGTCCCAAC TCAGCCATTG
TTAAAAGCCC ACCCTAAGTT TTCAAAGAAA TTTCACGACA ACGAGAAAGC AAGAGGCAAA
GCGATCCATC AAGCCAACCT TCGACATCTC TGCCGCATCT GTGGGAATTC TTTTAGAGCT
GATGAGCACA ACAGGAGATA TCCAGTCCAT GGTCCTGTGG ATGGTAAAAC CCTAGGCCTT
TTACGAAAGA AGGAAAAGAG AGCTACTTCC TGGCCGGACC TCATTGCCAA GGTTTTCCGG
ATCGATGTGA AGGCAGATGT TGACTCGATC CACCCCACTG AGTTCTGCCA TAACTGCTGG
AGCATCATGC ACAGGAAGTT TAGCAGTGCC CCATGTGAGG TTTACTTCCC GAGGAACGTG
ACCATGGAGT GGCACCCCCA CACACCATCC TGTGACATCT GCAACACTGC CCGTCGGGGA
CTCAAGAGGA AGAGTCTTCA GCCAAACTTG CAGCTCAGCA AAAAACTCAA AACTGTGCTT
GACCAAGCAA GACAAGCCCG TCAGCACAAG AGAAGAGCTC AGGCAAGGAT CAGCAGCAAG
GATGTCATGA AGAAGATCGC CAACTGCAGT AAGATACATC TTAGTACCAA GCTCCTTGCA
GTGGACTTCC CAGAGCACTT TGTGAAATCC ATCTCCTGCC AGATCTGTGA ACACATTCTG
GCTGACCCTG TGGAGACCAA CTGTAAGCAT GTCTTTTGCC GGGTCTGCAT TCTCAGATGC
CTCAAAGTCA TGGGCAGCTA TTGTCCCTCT TGCCGATATC CATGCTTCCC TACTGACCTG
GAGAGTCCAG TGAAGTCCTT TCTGAGCGTC TTGAATTCCC TGATGGTGAA ATGTCCAGCA
AAAGAGTGCA ATGAGGAGGT CAGTTTGGAA AAATATAATC ACCACATCTC AAGTCACAAG
GAATCAAAAG AGATTTTTGT GCACATTAAT AAAGGGGGCC GGCCCCGCCA ACATCTTCTG
TCGCTGACTC GGAGAGCTCA GAAGCACCGG CTGAGGGAGC TCAAGCTGCA AGTCAAAGCC
TTTGCTGACA AAGAAGAAGG TGGAGATGTG AAGTCCGTGT GCATGACCTT GTTCCTGCTG
GCTCTGAGGG CGAGGAATGA GCACAGGCAA GCTGATGAGC TGGAGGCCAT CATGCAGGGA
AAGGGCTCTG GCCTGCAGCC AGCTGTTTGC TTGGCCATCC GTGTCAACAC CTTCCTCAGC
TGCAGTCAGT ACCACAAGAT GTACAGGACT GTGAAAGCCA TCACAGGGAG ACAGATTTTT
CAGCCTTTGC ATGCCCTTCG GAATGCTGAG AAGGTACTTC TGCCAGGCTA CCACCACTTT
GAGTGGCAGC CACCTCTGAA GAATGTGTCT TCCAGCACTG ATGTTGGCAT TATTGATGGG
CTGTCTGGAC TATCATCCTC TGTGGATGAT TACCCAGTGG ACACCATTGC AAAGAGGTTC
CGCTATGATT CAGCTTTGGT GTCTGCTTTG ATGGACATGG AAGAAGACAT CTTGGAAGGC
ATGAGATCCC AAGACCTTGA TGATTACCTG AATGGCCCCT TCACTGTGGT GGTGAAGGAG
TCTTGTGATG GAATGGGAGA CGTGAGTGAG AAGCATGGGA GTGGGCCTGT AGTTCCAGAA
AAGGCAGTCC GTTTTTCATT CACAATCATG AAAATTACTA TTGCCCACAG CTCTCAGAAT
GTGAAAGTAT TTGAAGAAGC CAAACCTAAC TCTGAACTGT GTTGCAAGCC ATTGTGCCTT
ATGCTGGCAG ATGAGTCTGA CCACGAGACG CTGACTGCCA TCCTGAGTCC TCTCATTGCT
GAGAGGGAGG CCATGAAGAG CAGTGAATTA ATGCTTGAGC TGGGAGGCAT TCTCCGGACT
TTCAAGTTCA TCTTCAGGGG CACCGGCTAT GATGAAAAAC TTGTGCGGGA AGTGGAAGGC
CTCGAGGCTT CTGGCTCAGT CTACATTTGT ACTCTTTGTG ATGCCACCCG TCTGGAAGCC
TCTCAAAATC TTGTCTTCCA CTCTATAACC AGAAGCCATG CTGAGAACCT GGAACGTTAT
GAGGTCTGGC GTTCCAACCC TTACCATGAG TCTGTGGAAG AACTGCGGGA TCGGGTGAAA
GGGGTCTCAG CTAAACCTTT CATTGAGACA GTCCCTTCCA TAGATGCACT CCACTGTGAC
ATTGGCAATG CAGCTGAGTT CTACAAGATC TTCCAGCTAG AGATAGGGGA AGTGTATAAG
AATCCCAATG CTTCCAAAGA GGAAAGGAAA AGGTGGCAGG CCACACTGGA CAAGCATCTC
CGGAAGAAGA TGAACCTCAA ACCAATCATG AGGATGAATG GCAACTTTGC CAGGAAGCTC
ATGACCAAAG AGACTGTGGA TGCAGTTTGT GAGTTAATTC CTTCCGAGGA GAGGCACGAG
GCTCTGAGGG AGCTGATGGA TCTTTACCTG AAGATGAAAC CAGTATGGCG ATCATCATGC
CCTGCTAAAG AGTGCCCAGA ATCCCTCTGC CAGTACAGTT TCAATTCACA GCGTTTTGCT
GAGCTCCTTT CTACGAAGTT CAAGTATAGG TATGAGGGAA AAATCACCAA TTATTTTCAC
AAAACCCTGG CCCATGTTCC TGAAATTATT GAGAGGGATG GCTCCATTGG GGCATGGGCA
AGTGAGGGAA ATGAGTCTGG TAACAAACTG TTTAGGCGCT TCCGGAAAAT GAATGCCAGG
CAGTCCAAAT GCTATGAGAT GGAAGATGTC CTGAAACACC ACTGGTTGTA CACCTCCAAA
TACCTCCAGA AGTTTATGAA TGCTCATAAT GCATTAAAAA CCTCTGGGTT TACCATGAAC
CCTCAGGCAA GCTTAGGGGA CCCATTAGGC ATAGAGGACT CTCTGGAAAG CCAAGATTCA
ATGGAATTTT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005253098.1
CDS153..3284
Misc Feature(1)1029..1157(+)
Misc Feature(2)1029..1145(+)
Misc Feature(3)1212..1301(+)
Misc Feature(4)1689..3011(+)
Translation

Target ORF information:

RefSeq Version XM_005253041
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens recombination activating gene 1 (RAG1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005253041

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGGCAGCCT CTTTCCCACC CACCTTGGGA CTCAGTTCTG CCCCAGATGA AATTCAGCAC 
CCACATATTA AATTTTCAGA ATGGAAATTT AAGCTGTTCC GGGTGAGATC CTTTGAAAAG
ACACCTGAAG AAGCTCAAAA GGAAAAGAAG GATTCCTTTG AGGGGAAACC CTCTCTGGAG
CAATCTCCAG CAGTCCTGGA CAAGGCTGAT GGTCAGAAGC CAGTCCCAAC TCAGCCATTG
TTAAAAGCCC ACCCTAAGTT TTCAAAGAAA TTTCACGACA ACGAGAAAGC AAGAGGCAAA
GCGATCCATC AAGCCAACCT TCGACATCTC TGCCGCATCT GTGGGAATTC TTTTAGAGCT
GATGAGCACA ACAGGAGATA TCCAGTCCAT GGTCCTGTGG ATGGTAAAAC CCTAGGCCTT
TTACGAAAGA AGGAAAAGAG AGCTACTTCC TGGCCGGACC TCATTGCCAA GGTTTTCCGG
ATCGATGTGA AGGCAGATGT TGACTCGATC CACCCCACTG AGTTCTGCCA TAACTGCTGG
AGCATCATGC ACAGGAAGTT TAGCAGTGCC CCATGTGAGG TTTACTTCCC GAGGAACGTG
ACCATGGAGT GGCACCCCCA CACACCATCC TGTGACATCT GCAACACTGC CCGTCGGGGA
CTCAAGAGGA AGAGTCTTCA GCCAAACTTG CAGCTCAGCA AAAAACTCAA AACTGTGCTT
GACCAAGCAA GACAAGCCCG TCAGCACAAG AGAAGAGCTC AGGCAAGGAT CAGCAGCAAG
GATGTCATGA AGAAGATCGC CAACTGCAGT AAGATACATC TTAGTACCAA GCTCCTTGCA
GTGGACTTCC CAGAGCACTT TGTGAAATCC ATCTCCTGCC AGATCTGTGA ACACATTCTG
GCTGACCCTG TGGAGACCAA CTGTAAGCAT GTCTTTTGCC GGGTCTGCAT TCTCAGATGC
CTCAAAGTCA TGGGCAGCTA TTGTCCCTCT TGCCGATATC CATGCTTCCC TACTGACCTG
GAGAGTCCAG TGAAGTCCTT TCTGAGCGTC TTGAATTCCC TGATGGTGAA ATGTCCAGCA
AAAGAGTGCA ATGAGGAGGT CAGTTTGGAA AAATATAATC ACCACATCTC AAGTCACAAG
GAATCAAAAG AGATTTTTGT GCACATTAAT AAAGGGGGCC GGCCCCGCCA ACATCTTCTG
TCGCTGACTC GGAGAGCTCA GAAGCACCGG CTGAGGGAGC TCAAGCTGCA AGTCAAAGCC
TTTGCTGACA AAGAAGAAGG TGGAGATGTG AAGTCCGTGT GCATGACCTT GTTCCTGCTG
GCTCTGAGGG CGAGGAATGA GCACAGGCAA GCTGATGAGC TGGAGGCCAT CATGCAGGGA
AAGGGCTCTG GCCTGCAGCC AGCTGTTTGC TTGGCCATCC GTGTCAACAC CTTCCTCAGC
TGCAGTCAGT ACCACAAGAT GTACAGGACT GTGAAAGCCA TCACAGGGAG ACAGATTTTT
CAGCCTTTGC ATGCCCTTCG GAATGCTGAG AAGGTACTTC TGCCAGGCTA CCACCACTTT
GAGTGGCAGC CACCTCTGAA GAATGTGTCT TCCAGCACTG ATGTTGGCAT TATTGATGGG
CTGTCTGGAC TATCATCCTC TGTGGATGAT TACCCAGTGG ACACCATTGC AAAGAGGTTC
CGCTATGATT CAGCTTTGGT GTCTGCTTTG ATGGACATGG AAGAAGACAT CTTGGAAGGC
ATGAGATCCC AAGACCTTGA TGATTACCTG AATGGCCCCT TCACTGTGGT GGTGAAGGAG
TCTTGTGATG GAATGGGAGA CGTGAGTGAG AAGCATGGGA GTGGGCCTGT AGTTCCAGAA
AAGGCAGTCC GTTTTTCATT CACAATCATG AAAATTACTA TTGCCCACAG CTCTCAGAAT
GTGAAAGTAT TTGAAGAAGC CAAACCTAAC TCTGAACTGT GTTGCAAGCC ATTGTGCCTT
ATGCTGGCAG ATGAGTCTGA CCACGAGACG CTGACTGCCA TCCTGAGTCC TCTCATTGCT
GAGAGGGAGG CCATGAAGAG CAGTGAATTA ATGCTTGAGC TGGGAGGCAT TCTCCGGACT
TTCAAGTTCA TCTTCAGGGG CACCGGCTAT GATGAAAAAC TTGTGCGGGA AGTGGAAGGC
CTCGAGGCTT CTGGCTCAGT CTACATTTGT ACTCTTTGTG ATGCCACCCG TCTGGAAGCC
TCTCAAAATC TTGTCTTCCA CTCTATAACC AGAAGCCATG CTGAGAACCT GGAACGTTAT
GAGGTCTGGC GTTCCAACCC TTACCATGAG TCTGTGGAAG AACTGCGGGA TCGGGTGAAA
GGGGTCTCAG CTAAACCTTT CATTGAGACA GTCCCTTCCA TAGATGCACT CCACTGTGAC
ATTGGCAATG CAGCTGAGTT CTACAAGATC TTCCAGCTAG AGATAGGGGA AGTGTATAAG
AATCCCAATG CTTCCAAAGA GGAAAGGAAA AGGTGGCAGG CCACACTGGA CAAGCATCTC
CGGAAGAAGA TGAACCTCAA ACCAATCATG AGGATGAATG GCAACTTTGC CAGGAAGCTC
ATGACCAAAG AGACTGTGGA TGCAGTTTGT GAGTTAATTC CTTCCGAGGA GAGGCACGAG
GCTCTGAGGG AGCTGATGGA TCTTTACCTG AAGATGAAAC CAGTATGGCG ATCATCATGC
CCTGCTAAAG AGTGCCCAGA ATCCCTCTGC CAGTACAGTT TCAATTCACA GCGTTTTGCT
GAGCTCCTTT CTACGAAGTT CAAGTATAGG TATGAGGGAA AAATCACCAA TTATTTTCAC
AAAACCCTGG CCCATGTTCC TGAAATTATT GAGAGGGATG GCTCCATTGG GGCATGGGCA
AGTGAGGGAA ATGAGTCTGG TAACAAACTG TTTAGGCGCT TCCGGAAAAT GAATGCCAGG
CAGTCCAAAT GCTATGAGAT GGAAGATGTC CTGAAACACC ACTGGTTGTA CACCTCCAAA
TACCTCCAGA AGTTTATGAA TGCTCATAAT GCATTAAAAA CCTCTGGGTT TACCATGAAC
CCTCAGGCAA GCTTAGGGGA CCCATTAGGC ATAGAGGACT CTCTGGAAAG CCAAGATTCA
ATGGAATTTT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17854
Clone ID Related Accession (Same CDS sequence) NM_000448
Accession Version NM_000448.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3132bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product V(D)J recombination-activating protein 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC139427.3 and M29474.1. This sequence is a reference standard in the RefSeqGene project. On Apr 4, 2008 this sequence version replaced gi:4557840. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HY099452.1, M29474.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142853, SAMEA2144120 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGGCAGCCT CTTTCCCACC CACCTTGGGA CTCAGTTCTG CCCCAGATGA AATTCAGCAC 
CCACATATTA AATTTTCAGA ATGGAAATTT AAGCTGTTCC GGGTGAGATC CTTTGAAAAG
ACACCTGAAG AAGCTCAAAA GGAAAAGAAG GATTCCTTTG AGGGGAAACC CTCTCTGGAG
CAATCTCCAG CAGTCCTGGA CAAGGCTGAT GGTCAGAAGC CAGTCCCAAC TCAGCCATTG
TTAAAAGCCC ACCCTAAGTT TTCAAAGAAA TTTCACGACA ACGAGAAAGC AAGAGGCAAA
GCGATCCATC AAGCCAACCT TCGACATCTC TGCCGCATCT GTGGGAATTC TTTTAGAGCT
GATGAGCACA ACAGGAGATA TCCAGTCCAT GGTCCTGTGG ATGGTAAAAC CCTAGGCCTT
TTACGAAAGA AGGAAAAGAG AGCTACTTCC TGGCCGGACC TCATTGCCAA GGTTTTCCGG
ATCGATGTGA AGGCAGATGT TGACTCGATC CACCCCACTG AGTTCTGCCA TAACTGCTGG
AGCATCATGC ACAGGAAGTT TAGCAGTGCC CCATGTGAGG TTTACTTCCC GAGGAACGTG
ACCATGGAGT GGCACCCCCA CACACCATCC TGTGACATCT GCAACACTGC CCGTCGGGGA
CTCAAGAGGA AGAGTCTTCA GCCAAACTTG CAGCTCAGCA AAAAACTCAA AACTGTGCTT
GACCAAGCAA GACAAGCCCG TCAGCGCAAG AGAAGAGCTC AGGCAAGGAT CAGCAGCAAG
GATGTCATGA AGAAGATCGC CAACTGCAGT AAGATACATC TTAGTACCAA GCTCCTTGCA
GTGGACTTCC CAGAGCACTT TGTGAAATCC ATCTCCTGCC AGATCTGTGA ACACATTCTG
GCTGACCCTG TGGAGACCAA CTGTAAGCAT GTCTTTTGCC GGGTCTGCAT TCTCAGATGC
CTCAAAGTCA TGGGCAGCTA TTGTCCCTCT TGCCGATATC CATGCTTCCC TACTGACCTG
GAGAGTCCAG TGAAGTCCTT TCTGAGCGTC TTGAATTCCC TGATGGTGAA ATGTCCAGCA
AAAGAGTGCA ATGAGGAGGT CAGTTTGGAA AAATATAATC ACCACATCTC AAGTCACAAG
GAATCAAAAG AGATTTTTGT GCACATTAAT AAAGGGGGCC GGCCCCGCCA ACATCTTCTG
TCGCTGACTC GGAGAGCTCA GAAGCACCGG CTGAGGGAGC TCAAGCTGCA AGTCAAAGCC
TTTGCTGACA AAGAAGAAGG TGGAGATGTG AAGTCCGTGT GCATGACCTT GTTCCTGCTG
GCTCTGAGGG CGAGGAATGA GCACAGGCAA GCTGATGAGC TGGAGGCCAT CATGCAGGGA
AAGGGCTCTG GCCTGCAGCC AGCTGTTTGC TTGGCCATCC GTGTCAACAC CTTCCTCAGC
TGCAGTCAGT ACCACAAGAT GTACAGGACT GTGAAAGCCA TCACAGGGAG ACAGATTTTT
CAGCCTTTGC ATGCCCTTCG GAATGCTGAG AAGGTACTTC TGCCAGGCTA CCACCACTTT
GAGTGGCAGC CACCTCTGAA GAATGTGTCT TCCAGCACTG ATGTTGGCAT TATTGATGGG
CTGTCTGGAC TATCATCCTC TGTGGATGAT TACCCAGTGG ACACCATTGC AAAGAGGTTC
CGCTATGATT CAGCTTTGGT GTCTGCTTTG ATGGACATGG AAGAAGACAT CTTGGAAGGC
ATGAGATCCC AAGACCTTGA TGATTACCTG AATGGCCCCT TCACTGTGGT GGTGAAGGAG
TCTTGTGATG GAATGGGAGA CGTGAGTGAG AAGCATGGGA GTGGGCCTGT AGTTCCAGAA
AAGGCAGTCC GTTTTTCATT CACAATCATG AAAATTACTA TTGCCCACAG CTCTCAGAAT
GTGAAAGTAT TTGAAGAAGC CAAACCTAAC TCTGAACTGT GTTGCAAGCC ATTGTGCCTT
ATGCTGGCAG ATGAGTCTGA CCACGAGACG CTGACTGCCA TCCTGAGTCC TCTCATTGCT
GAGAGGGAGG CCATGAAGAG CAGTGAATTA ATGCTTGAGC TGGGAGGCAT TCTCCGGACT
TTCAAGTTCA TCTTCAGGGG CACCGGCTAT GATGAAAAAC TTGTGCGGGA AGTGGAAGGC
CTCGAGGCTT CTGGCTCAGT CTACATTTGT ACTCTTTGTG ATGCCACCCG TCTGGAAGCC
TCTCAAAATC TTGTCTTCCA CTCTATAACC AGAAGCCATG CTGAGAACCT GGAACGTTAT
GAGGTCTGGC GTTCCAACCC TTACCATGAG TCTGTGGAAG AACTGCGGGA TCGGGTGAAA
GGGGTCTCAG CTAAACCTTT CATTGAGACA GTCCCTTCCA TAGATGCACT CCACTGTGAC
ATTGGCAATG CAGCTGAGTT CTACAAGATC TTCCAGCTAG AGATAGGGGA AGTGTATAAG
AATCCCAATG CTTCCAAAGA GGAAAGGAAA AGGTGGCAGG CCACACTGGA CAAGCATCTC
CGGAAGAAGA TGAACCTCAA ACCAATCATG AGGATGAATG GCAACTTTGC CAGGAAGCTC
ATGACCAAAG AGACTGTGGA TGCAGTTTGT GAGTTAATTC CTTCCGAGGA GAGGCACGAG
GCTCTGAGGG AGCTGATGGA TCTTTACCTG AAGATGAAAC CAGTATGGCG ATCATCATGC
CCTGCTAAAG AGTGCCCAGA ATCCCTCTGC CAGTACAGTT TCAATTCACA GCGTTTTGCT
GAGCTCCTTT CTACGAAGTT CAAGTATAGG TATGAGGGAA AAATCACCAA TTATTTTCAC
AAAACCCTGG CCCATGTTCC TGAAATTATT GAGAGGGATG GCTCCATTGG GGCATGGGCA
AGTGAGGGAA ATGAGTCTGG TAACAAACTG TTTAGGCGCT TCCGGAAAAT GAATGCCAGG
CAGTCCAAAT GCTATGAGAT GGAAGATGTC CTGAAACACC ACTGGTTGTA CACCTCCAAA
TACCTCCAGA AGTTTATGAA TGCTCATAAT GCATTAAAAA CCTCTGGGTT TACCATGAAC
CCTCAGGCAA GCTTAGGGGA CCCATTAGGC ATAGAGGACT CTCTGGAAAG CCAAGATTCA
ATGGAATTTT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000439.1
CDS125..3256
Misc Feature(1)53..55(+)
Misc Feature(2)125..988(+)
Misc Feature(3)1001..1129(+)
Misc Feature(4)1001..1117(+)
Misc Feature(5)1184..1273(+)
Misc Feature(6)1661..2983(+)
Misc Feature(7)2810..2812(+)
Exon (1)1..110
Gene:RAG1
Gene Synonym:
Exon (2)111..6582
Gene:RAG1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000448
Organism Homo sapiens (human)
Definition Homo sapiens recombination activating gene 1 (RAG1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000448

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGGCAGCCT CTTTCCCACC CACCTTGGGA CTCAGTTCTG CCCCAGATGA AATTCAGCAC 
CCACATATTA AATTTTCAGA ATGGAAATTT AAGCTGTTCC GGGTGAGATC CTTTGAAAAG
ACACCTGAAG AAGCTCAAAA GGAAAAGAAG GATTCCTTTG AGGGGAAACC CTCTCTGGAG
CAATCTCCAG CAGTCCTGGA CAAGGCTGAT GGTCAGAAGC CAGTCCCAAC TCAGCCATTG
TTAAAAGCCC ACCCTAAGTT TTCAAAGAAA TTTCACGACA ACGAGAAAGC AAGAGGCAAA
GCGATCCATC AAGCCAACCT TCGACATCTC TGCCGCATCT GTGGGAATTC TTTTAGAGCT
GATGAGCACA ACAGGAGATA TCCAGTCCAT GGTCCTGTGG ATGGTAAAAC CCTAGGCCTT
TTACGAAAGA AGGAAAAGAG AGCTACTTCC TGGCCGGACC TCATTGCCAA GGTTTTCCGG
ATCGATGTGA AGGCAGATGT TGACTCGATC CACCCCACTG AGTTCTGCCA TAACTGCTGG
AGCATCATGC ACAGGAAGTT TAGCAGTGCC CCATGTGAGG TTTACTTCCC GAGGAACGTG
ACCATGGAGT GGCACCCCCA CACACCATCC TGTGACATCT GCAACACTGC CCGTCGGGGA
CTCAAGAGGA AGAGTCTTCA GCCAAACTTG CAGCTCAGCA AAAAACTCAA AACTGTGCTT
GACCAAGCAA GACAAGCCCG TCAGCGCAAG AGAAGAGCTC AGGCAAGGAT CAGCAGCAAG
GATGTCATGA AGAAGATCGC CAACTGCAGT AAGATACATC TTAGTACCAA GCTCCTTGCA
GTGGACTTCC CAGAGCACTT TGTGAAATCC ATCTCCTGCC AGATCTGTGA ACACATTCTG
GCTGACCCTG TGGAGACCAA CTGTAAGCAT GTCTTTTGCC GGGTCTGCAT TCTCAGATGC
CTCAAAGTCA TGGGCAGCTA TTGTCCCTCT TGCCGATATC CATGCTTCCC TACTGACCTG
GAGAGTCCAG TGAAGTCCTT TCTGAGCGTC TTGAATTCCC TGATGGTGAA ATGTCCAGCA
AAAGAGTGCA ATGAGGAGGT CAGTTTGGAA AAATATAATC ACCACATCTC AAGTCACAAG
GAATCAAAAG AGATTTTTGT GCACATTAAT AAAGGGGGCC GGCCCCGCCA ACATCTTCTG
TCGCTGACTC GGAGAGCTCA GAAGCACCGG CTGAGGGAGC TCAAGCTGCA AGTCAAAGCC
TTTGCTGACA AAGAAGAAGG TGGAGATGTG AAGTCCGTGT GCATGACCTT GTTCCTGCTG
GCTCTGAGGG CGAGGAATGA GCACAGGCAA GCTGATGAGC TGGAGGCCAT CATGCAGGGA
AAGGGCTCTG GCCTGCAGCC AGCTGTTTGC TTGGCCATCC GTGTCAACAC CTTCCTCAGC
TGCAGTCAGT ACCACAAGAT GTACAGGACT GTGAAAGCCA TCACAGGGAG ACAGATTTTT
CAGCCTTTGC ATGCCCTTCG GAATGCTGAG AAGGTACTTC TGCCAGGCTA CCACCACTTT
GAGTGGCAGC CACCTCTGAA GAATGTGTCT TCCAGCACTG ATGTTGGCAT TATTGATGGG
CTGTCTGGAC TATCATCCTC TGTGGATGAT TACCCAGTGG ACACCATTGC AAAGAGGTTC
CGCTATGATT CAGCTTTGGT GTCTGCTTTG ATGGACATGG AAGAAGACAT CTTGGAAGGC
ATGAGATCCC AAGACCTTGA TGATTACCTG AATGGCCCCT TCACTGTGGT GGTGAAGGAG
TCTTGTGATG GAATGGGAGA CGTGAGTGAG AAGCATGGGA GTGGGCCTGT AGTTCCAGAA
AAGGCAGTCC GTTTTTCATT CACAATCATG AAAATTACTA TTGCCCACAG CTCTCAGAAT
GTGAAAGTAT TTGAAGAAGC CAAACCTAAC TCTGAACTGT GTTGCAAGCC ATTGTGCCTT
ATGCTGGCAG ATGAGTCTGA CCACGAGACG CTGACTGCCA TCCTGAGTCC TCTCATTGCT
GAGAGGGAGG CCATGAAGAG CAGTGAATTA ATGCTTGAGC TGGGAGGCAT TCTCCGGACT
TTCAAGTTCA TCTTCAGGGG CACCGGCTAT GATGAAAAAC TTGTGCGGGA AGTGGAAGGC
CTCGAGGCTT CTGGCTCAGT CTACATTTGT ACTCTTTGTG ATGCCACCCG TCTGGAAGCC
TCTCAAAATC TTGTCTTCCA CTCTATAACC AGAAGCCATG CTGAGAACCT GGAACGTTAT
GAGGTCTGGC GTTCCAACCC TTACCATGAG TCTGTGGAAG AACTGCGGGA TCGGGTGAAA
GGGGTCTCAG CTAAACCTTT CATTGAGACA GTCCCTTCCA TAGATGCACT CCACTGTGAC
ATTGGCAATG CAGCTGAGTT CTACAAGATC TTCCAGCTAG AGATAGGGGA AGTGTATAAG
AATCCCAATG CTTCCAAAGA GGAAAGGAAA AGGTGGCAGG CCACACTGGA CAAGCATCTC
CGGAAGAAGA TGAACCTCAA ACCAATCATG AGGATGAATG GCAACTTTGC CAGGAAGCTC
ATGACCAAAG AGACTGTGGA TGCAGTTTGT GAGTTAATTC CTTCCGAGGA GAGGCACGAG
GCTCTGAGGG AGCTGATGGA TCTTTACCTG AAGATGAAAC CAGTATGGCG ATCATCATGC
CCTGCTAAAG AGTGCCCAGA ATCCCTCTGC CAGTACAGTT TCAATTCACA GCGTTTTGCT
GAGCTCCTTT CTACGAAGTT CAAGTATAGG TATGAGGGAA AAATCACCAA TTATTTTCAC
AAAACCCTGG CCCATGTTCC TGAAATTATT GAGAGGGATG GCTCCATTGG GGCATGGGCA
AGTGAGGGAA ATGAGTCTGG TAACAAACTG TTTAGGCGCT TCCGGAAAAT GAATGCCAGG
CAGTCCAAAT GCTATGAGAT GGAAGATGTC CTGAAACACC ACTGGTTGTA CACCTCCAAA
TACCTCCAGA AGTTTATGAA TGCTCATAAT GCATTAAAAA CCTCTGGGTT TACCATGAAC
CCTCAGGCAA GCTTAGGGGA CCCATTAGGC ATAGAGGACT CTCTGGAAAG CCAAGATTCA
ATGGAATTTT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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