RASGRF1 cDNA ORF clone, Homo sapiens(human)

The following RASGRF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASGRF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu08459 NM_153815.2
Latest version!
Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $286.30
$409.00
OHu85582 XM_017022455.2
Latest version!
Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu85583 XM_017022456.2
Latest version!
Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu85584 XM_017022457.2
Latest version!
Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu85585 XM_017022458.2
Latest version!
Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu85586 XM_017022459.2
Latest version!
Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu08312 NM_001145648.2
Latest version!
Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $888.30
$1269.00
OHu08508 NM_002891.5
Latest version!
Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu60207 XM_011521866.3
Latest version!
Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu60208 XM_011521867.3
Latest version!
Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
View Old Accession Versions >>
OHu08508 NM_002891.4 Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu60208 XM_011521867.2 Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu85585 XM_017022458.1 Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu85586 XM_017022459.1 Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu08312 NM_001145648.1 Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $888.30
$1269.00
OHu85582 XM_017022455.1 Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu85583 XM_017022456.1 Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu85584 XM_017022457.1 Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu60207 XM_011521866.2 Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu08459
    Clone ID Related Accession (Same CDS sequence) NM_153815.2
    Accession Version NM_153815.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1470bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-03
    Organism Homo sapiens(human)
    Product ras-specific guanine nucleotide-releasing factor 1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011944.12, AK226101.1 and AL355696.1. On Mar 6, 2009 this sequence version replaced NM_153815.1. Transcript Variant: This variant (2) lacks multiple 5' exon, but has an alternate 5' exon, as compared to variant 1. The resulting isoform (2) has a much shorter N-terminus, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: S62035.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTACTCGG CCATGTCACC CTTCAGCAAG GCCACGCTGG ACACCAGCAA GCTCTATGTG 
    TCCAGCAGCT TCACCAACAA GATTCCAGAT GAGGGCGATA CGACCCCTGA GAAGCCCGAA
    GACCCTTCAG CGCTCAGCAA GCAGAGCTCA GAAGTCTCCA TGAGAGAGGA GTCAGATATT
    GATCAAAACC AGAGTGATGA TGGTGATACT GAAACATCAC CAACTAAATC TCCAACAACA
    CCCAAATCAG TCAAAAACAA AAATTCTTCA GAGTTCCCAC TCTTTTCCTA TAACAATGGA
    GTCGTCATGA CCTCCTGTCG TGAACTGGAC AATAACCGCA GTGCCTTGTC GGCCGCCTCT
    GCCTTTGCCA TAGCAACCGC CGGGGCCAAC GAGGGCACCC CAAACAAGGA GAAGTACCGG
    AGGATGTCCT TAGCCAGTGC AGGGTTTCCC CCAGACCAGA GGAATGGAGA CAAGGAGTTT
    GTGATCCGCA GAGCAGCCAC CAATCGTGTC TTGAACGTGC TCCGCCACTG GGTGTCCAAG
    CACTCTCAGG ACTTTGAGAC CAACGATGAG CTCAAATGCA AGGTGATCGG CTTCCTGGAA
    GAAGTCATGC ACGACCCGGA GCTCCTGACC CAGGAGCGGA AGGCTGCAGC CAACATCATC
    AGGACTCTGA CCCAGGAGGA CCCAGGTGAC AACCAGATCA CGCTGGAGGA GATCACGCAG
    ATGGCTGAAG GCGTGAAGGC TGAGCCCTTT GAAAACCACT CAGCCCTGGA GATCGCGGAG
    CAGCTGACCC TGCTAGATCA CCTCGTCTTC AAGAAGATTC CTTATGAGGA GTTCTTCGGA
    CAAGGATGGA TGAAACTGGA AAAGAATGAA AGGACCCCTT ATATCATGAA AACCACTAAG
    CACTTCAATG ACATCAGTAA CTTGATTGCT TCAGAAATCA TCCGCAATGA GGACATCAAC
    GCCAGGGTGA GCGCCATCGA GAAGTGGGTG GCCGTAGCTG ACATATGCCG CTGCCTCCAC
    AACTACAATG CCGTACTGGA GATCACCTCG TCCATGAACC GCAGTGCAAT CTTCCGGCTC
    AAAAAGACGT GGCTCAAAGT CTCTAAGCAG ACTAAAGCTT TGATTGATAA GCTCCAAAAG
    CTTGTGTCAT CTGAGGGCAG ATTTAAGAAT CTCAGAGAAG CTCTGAAAAA TTGTGACCCA
    CCCTGTGTCC CTTACCTGGG GATGTACCTC ACCGACCTGG CCTTCATCGA GGAGGGGACG
    CCCAATTACA CGGAAGACGG CCTGGTCAAC TTCTCCAAGA TGAGGATGAT ATCCCATATT
    ATCCGAGAGA TTCGCCAGTT TCAACAAACT GCCTACAAAA TAGAGCACCA AGCAAAGGTA
    ACGCAATATT TACTGGACCA ATCTTTTGTA ATGGATGAAG AAAGCCTCTA CGAGTCTTCT
    CTCCGAATAG AACCAAAACT CCCCACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_722522.1
    CDS1174..2643
    Translation

    Target ORF information:

    RefSeq Version NM_153815.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_153815.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTACTCGG CCATGTCACC CTTCAGCAAG GCCACGCTGG ACACCAGCAA GCTCTATGTG 
    TCCAGCAGCT TCACCAACAA GATTCCAGAT GAGGGCGATA CGACCCCTGA GAAGCCCGAA
    GACCCTTCAG CGCTCAGCAA GCAGAGCTCA GAAGTCTCCA TGAGAGAGGA GTCAGATATT
    GATCAAAACC AGAGTGATGA TGGTGATACT GAAACATCAC CAACTAAATC TCCAACAACA
    CCCAAATCAG TCAAAAACAA AAATTCTTCA GAGTTCCCAC TCTTTTCCTA TAACAATGGA
    GTCGTCATGA CCTCCTGTCG TGAACTGGAC AATAACCGCA GTGCCTTGTC GGCCGCCTCT
    GCCTTTGCCA TAGCAACCGC CGGGGCCAAC GAGGGCACCC CAAACAAGGA GAAGTACCGG
    AGGATGTCCT TAGCCAGTGC AGGGTTTCCC CCAGACCAGA GGAATGGAGA CAAGGAGTTT
    GTGATCCGCA GAGCAGCCAC CAATCGTGTC TTGAACGTGC TCCGCCACTG GGTGTCCAAG
    CACTCTCAGG ACTTTGAGAC CAACGATGAG CTCAAATGCA AGGTGATCGG CTTCCTGGAA
    GAAGTCATGC ACGACCCGGA GCTCCTGACC CAGGAGCGGA AGGCTGCAGC CAACATCATC
    AGGACTCTGA CCCAGGAGGA CCCAGGTGAC AACCAGATCA CGCTGGAGGA GATCACGCAG
    ATGGCTGAAG GCGTGAAGGC TGAGCCCTTT GAAAACCACT CAGCCCTGGA GATCGCGGAG
    CAGCTGACCC TGCTAGATCA CCTCGTCTTC AAGAAGATTC CTTATGAGGA GTTCTTCGGA
    CAAGGATGGA TGAAACTGGA AAAGAATGAA AGGACCCCTT ATATCATGAA AACCACTAAG
    CACTTCAATG ACATCAGTAA CTTGATTGCT TCAGAAATCA TCCGCAATGA GGACATCAAC
    GCCAGGGTGA GCGCCATCGA GAAGTGGGTG GCCGTAGCTG ACATATGCCG CTGCCTCCAC
    AACTACAATG CCGTACTGGA GATCACCTCG TCCATGAACC GCAGTGCAAT CTTCCGGCTC
    AAAAAGACGT GGCTCAAAGT CTCTAAGCAG ACTAAAGCTT TGATTGATAA GCTCCAAAAG
    CTTGTGTCAT CTGAGGGCAG ATTTAAGAAT CTCAGAGAAG CTCTGAAAAA TTGTGACCCA
    CCCTGTGTCC CTTACCTGGG GATGTACCTC ACCGACCTGG CCTTCATCGA GGAGGGGACG
    CCCAATTACA CGGAAGACGG CCTGGTCAAC TTCTCCAAGA TGAGGATGAT ATCCCATATT
    ATCCGAGAGA TTCGCCAGTT TCAACAAACT GCCTACAAAA TAGAGCACCA AGCAAAGGTA
    ACGCAATATT TACTGGACCA ATCTTTTGTA ATGGATGAAG AAAGCCTCTA CGAGTCTTCT
    CTCCGAATAG AACCAAAACT CCCCACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85582
    Clone ID Related Accession (Same CDS sequence) XM_017022455.2 , XM_017022455.1
    Accession Version XM_017022455.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3813bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ras-specific guanine nucleotide-releasing factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017022455.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGAGG ACCAGGGAGG GGACCAGGGA AGCAGAAATG
    AGCAGGTCCG ACGCCTCCTT ATATTGTGAT GATGTTGACA TTCGCTTCAG CAAAACCATG
    AACTCCTGCA AAGTGCTGCA GATCCGCTAC GCCAGTGTGG AGCGGCTGCT GGAGAGGCTG
    ACGGACCTGC GCTTCCTGAG CATCGACTTC CTCAACACCT TCCTGCACTC CTACCGCGTC
    TTCACCACCG CCATCGTGGT CCTGGACAAG CTCATTACCA TCTACAAGAA GCCTATCAGT
    GCCATTCCTG CCAGGTCGCT GGAGCTCCTG TTTGCCAGTG GCCAGAACAA TAAGCTCCTG
    TACGGTGAAC CCCCCAAGTC CCCGCGCGCC ACCCGCAAGT TCTCCTCGCC GCCACCTCTG
    TCCATCACCA AGACATCGTC ACCGAGCCGC CGGCGGAAGC TCTCCCTGAA CATCCCCATC
    ATCACTGGCG GCAAGGCCCT GGACCTGGCC GCCCTCAGCT GCAACTCCAA TGGCTACACC
    AGCATGTACT CGGCCATGTC ACCCTTCAGC AAGGCCACGC TGGACACCAG CAAGCTCTAT
    GTGTCCAGCA GCTTCACCAA CAAGATTCCA GATGAGGGCG ATACGACCCC TGAGAAGCCC
    GAAGACCCTT CAGCGCTCAG CAAGCAGAGC TCAGAAGTCT CCATGAGAGA GGAGTCAGAT
    ATTGATCAAA ACCAGAGTGA TGATGGTGAT ACTGAAACAT CACCAACTAA ATCTCCAACA
    ACACCCAAAT CAGTCAAAAA CAAAAATTCT TCAGAGTTCC CACTCTTTTC CTATAACAAT
    GGAGTCGTCA TGACCTCCTG TCGTGAACTG GACAATAACC GCAGTGCCTT GTCGGCCGCC
    TCTGCCTTTG CCATAGCAAC CGCCGGGGCC AACGAGGGCA CCCCAAACAA GGAGAAGTAC
    CGGAGGATGT CCTTAGCCAG TGCAGGGTTT CCCCCAGACC AGAGGAATGG AGACAAGGAG
    TTTGTGATCC GCAGAGCAGC CACCAATCGT GTCTTGAACG TGCTCCGCCA CTGGGTGTCC
    AAGCACTCTC AGGACTTTGA GACCAACGAT GAGCTCAAAT GCAAGGTGAT CGGCTTCCTG
    GAAGAAGTCA TGCACGACCC GGAGCTCCTG ACCCAGGAGC GGAAGGCTGC AGCCAACATC
    ATCAGGACTC TGACCCAGGA GGACCCAGGT GACAACCAGA TCACGCTGGA GGAGATCACG
    CAGATGGCTG AAGGCGTGAA GGCTGAGCCC TTTGAAAACC ACTCAGCCCT GGAGATCGCG
    GAGCAGCTGA CCCTGCTAGA TCACCTCGTC TTCAAGAAGA TTCCTTATGA GGAGTTCTTC
    GGACAAGGAT GGATGAAACT GGAAAAGAAT GAAAGGACCC CTTATATCAT GAAAACCACT
    AAGCACTTCA ATGACATCAG TAACTTGATT GCTTCAGAAA TCATCCGCAA TGAGGACATC
    AACGCCAGGG TGAGCGCCAT CGAGAAGTGG GTGGCCGTAG CTGACATATG CCGCTGCCTC
    CACAACTACA ATGCCGTACT GGAGATCACC TCGTCCATGA ACCGCAGTGC AATCTTCCGG
    CTCAAAAAGA CGTGGCTCAA AGTCTCTAAG CAGACTAAAG CTTTGATTGA TAAGCTCCAA
    AAGCTTGTGT CATCTGAGGG CAGATTTAAG AATCTCAGAG AAGCTCTGAA AAATTGTGAC
    CCACCCTGTG TCCCTTACCT GGGGATGTAC CTCACCGACC TGGCCTTCAT CGAGGAGGGG
    ACGCCCAATT ACACGGAAGA CGGCCTGGTC AACTTCTCCA AGATGAGGAT GATATCCCAT
    ATTATCCGAG AGATTCGCCA GTTTCAACAA ACTGCCTACA AAATAGAGCA CCAAGCAAAG
    GTAACGCAAT ATTTACTGGA CCAATCTTTT GTAATGGATG AAGAAAGCCT CTACGAGTCT
    TCTCTCCGAA TAGAACCAAA ACTCCCCACC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016877944.1
    CDS275..4087
    Translation

    Target ORF information:

    RefSeq Version XM_017022455.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022455.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGAGG ACCAGGGAGG GGACCAGGGA AGCAGAAATG
    AGCAGGTCCG ACGCCTCCTT ATATTGTGAT GATGTTGACA TTCGCTTCAG CAAAACCATG
    AACTCCTGCA AAGTGCTGCA GATCCGCTAC GCCAGTGTGG AGCGGCTGCT GGAGAGGCTG
    ACGGACCTGC GCTTCCTGAG CATCGACTTC CTCAACACCT TCCTGCACTC CTACCGCGTC
    TTCACCACCG CCATCGTGGT CCTGGACAAG CTCATTACCA TCTACAAGAA GCCTATCAGT
    GCCATTCCTG CCAGGTCGCT GGAGCTCCTG TTTGCCAGTG GCCAGAACAA TAAGCTCCTG
    TACGGTGAAC CCCCCAAGTC CCCGCGCGCC ACCCGCAAGT TCTCCTCGCC GCCACCTCTG
    TCCATCACCA AGACATCGTC ACCGAGCCGC CGGCGGAAGC TCTCCCTGAA CATCCCCATC
    ATCACTGGCG GCAAGGCCCT GGACCTGGCC GCCCTCAGCT GCAACTCCAA TGGCTACACC
    AGCATGTACT CGGCCATGTC ACCCTTCAGC AAGGCCACGC TGGACACCAG CAAGCTCTAT
    GTGTCCAGCA GCTTCACCAA CAAGATTCCA GATGAGGGCG ATACGACCCC TGAGAAGCCC
    GAAGACCCTT CAGCGCTCAG CAAGCAGAGC TCAGAAGTCT CCATGAGAGA GGAGTCAGAT
    ATTGATCAAA ACCAGAGTGA TGATGGTGAT ACTGAAACAT CACCAACTAA ATCTCCAACA
    ACACCCAAAT CAGTCAAAAA CAAAAATTCT TCAGAGTTCC CACTCTTTTC CTATAACAAT
    GGAGTCGTCA TGACCTCCTG TCGTGAACTG GACAATAACC GCAGTGCCTT GTCGGCCGCC
    TCTGCCTTTG CCATAGCAAC CGCCGGGGCC AACGAGGGCA CCCCAAACAA GGAGAAGTAC
    CGGAGGATGT CCTTAGCCAG TGCAGGGTTT CCCCCAGACC AGAGGAATGG AGACAAGGAG
    TTTGTGATCC GCAGAGCAGC CACCAATCGT GTCTTGAACG TGCTCCGCCA CTGGGTGTCC
    AAGCACTCTC AGGACTTTGA GACCAACGAT GAGCTCAAAT GCAAGGTGAT CGGCTTCCTG
    GAAGAAGTCA TGCACGACCC GGAGCTCCTG ACCCAGGAGC GGAAGGCTGC AGCCAACATC
    ATCAGGACTC TGACCCAGGA GGACCCAGGT GACAACCAGA TCACGCTGGA GGAGATCACG
    CAGATGGCTG AAGGCGTGAA GGCTGAGCCC TTTGAAAACC ACTCAGCCCT GGAGATCGCG
    GAGCAGCTGA CCCTGCTAGA TCACCTCGTC TTCAAGAAGA TTCCTTATGA GGAGTTCTTC
    GGACAAGGAT GGATGAAACT GGAAAAGAAT GAAAGGACCC CTTATATCAT GAAAACCACT
    AAGCACTTCA ATGACATCAG TAACTTGATT GCTTCAGAAA TCATCCGCAA TGAGGACATC
    AACGCCAGGG TGAGCGCCAT CGAGAAGTGG GTGGCCGTAG CTGACATATG CCGCTGCCTC
    CACAACTACA ATGCCGTACT GGAGATCACC TCGTCCATGA ACCGCAGTGC AATCTTCCGG
    CTCAAAAAGA CGTGGCTCAA AGTCTCTAAG CAGACTAAAG CTTTGATTGA TAAGCTCCAA
    AAGCTTGTGT CATCTGAGGG CAGATTTAAG AATCTCAGAG AAGCTCTGAA AAATTGTGAC
    CCACCCTGTG TCCCTTACCT GGGGATGTAC CTCACCGACC TGGCCTTCAT CGAGGAGGGG
    ACGCCCAATT ACACGGAAGA CGGCCTGGTC AACTTCTCCA AGATGAGGAT GATATCCCAT
    ATTATCCGAG AGATTCGCCA GTTTCAACAA ACTGCCTACA AAATAGAGCA CCAAGCAAAG
    GTAACGCAAT ATTTACTGGA CCAATCTTTT GTAATGGATG AAGAAAGCCT CTACGAGTCT
    TCTCTCCGAA TAGAACCAAA ACTCCCCACC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85582
    Clone ID Related Accession (Same CDS sequence) XM_017022455.2 , XM_017022455.1
    Accession Version XM_017022455.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3813bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ras-specific guanine nucleotide-releasing factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGAGG ACCAGGGAGG GGACCAGGGA AGCAGAAATG
    AGCAGGTCCG ACGCCTCCTT ATATTGTGAT GATGTTGACA TTCGCTTCAG CAAAACCATG
    AACTCCTGCA AAGTGCTGCA GATCCGCTAC GCCAGTGTGG AGCGGCTGCT GGAGAGGCTG
    ACGGACCTGC GCTTCCTGAG CATCGACTTC CTCAACACCT TCCTGCACTC CTACCGCGTC
    TTCACCACCG CCATCGTGGT CCTGGACAAG CTCATTACCA TCTACAAGAA GCCTATCAGT
    GCCATTCCTG CCAGGTCGCT GGAGCTCCTG TTTGCCAGTG GCCAGAACAA TAAGCTCCTG
    TACGGTGAAC CCCCCAAGTC CCCGCGCGCC ACCCGCAAGT TCTCCTCGCC GCCACCTCTG
    TCCATCACCA AGACATCGTC ACCGAGCCGC CGGCGGAAGC TCTCCCTGAA CATCCCCATC
    ATCACTGGCG GCAAGGCCCT GGACCTGGCC GCCCTCAGCT GCAACTCCAA TGGCTACACC
    AGCATGTACT CGGCCATGTC ACCCTTCAGC AAGGCCACGC TGGACACCAG CAAGCTCTAT
    GTGTCCAGCA GCTTCACCAA CAAGATTCCA GATGAGGGCG ATACGACCCC TGAGAAGCCC
    GAAGACCCTT CAGCGCTCAG CAAGCAGAGC TCAGAAGTCT CCATGAGAGA GGAGTCAGAT
    ATTGATCAAA ACCAGAGTGA TGATGGTGAT ACTGAAACAT CACCAACTAA ATCTCCAACA
    ACACCCAAAT CAGTCAAAAA CAAAAATTCT TCAGAGTTCC CACTCTTTTC CTATAACAAT
    GGAGTCGTCA TGACCTCCTG TCGTGAACTG GACAATAACC GCAGTGCCTT GTCGGCCGCC
    TCTGCCTTTG CCATAGCAAC CGCCGGGGCC AACGAGGGCA CCCCAAACAA GGAGAAGTAC
    CGGAGGATGT CCTTAGCCAG TGCAGGGTTT CCCCCAGACC AGAGGAATGG AGACAAGGAG
    TTTGTGATCC GCAGAGCAGC CACCAATCGT GTCTTGAACG TGCTCCGCCA CTGGGTGTCC
    AAGCACTCTC AGGACTTTGA GACCAACGAT GAGCTCAAAT GCAAGGTGAT CGGCTTCCTG
    GAAGAAGTCA TGCACGACCC GGAGCTCCTG ACCCAGGAGC GGAAGGCTGC AGCCAACATC
    ATCAGGACTC TGACCCAGGA GGACCCAGGT GACAACCAGA TCACGCTGGA GGAGATCACG
    CAGATGGCTG AAGGCGTGAA GGCTGAGCCC TTTGAAAACC ACTCAGCCCT GGAGATCGCG
    GAGCAGCTGA CCCTGCTAGA TCACCTCGTC TTCAAGAAGA TTCCTTATGA GGAGTTCTTC
    GGACAAGGAT GGATGAAACT GGAAAAGAAT GAAAGGACCC CTTATATCAT GAAAACCACT
    AAGCACTTCA ATGACATCAG TAACTTGATT GCTTCAGAAA TCATCCGCAA TGAGGACATC
    AACGCCAGGG TGAGCGCCAT CGAGAAGTGG GTGGCCGTAG CTGACATATG CCGCTGCCTC
    CACAACTACA ATGCCGTACT GGAGATCACC TCGTCCATGA ACCGCAGTGC AATCTTCCGG
    CTCAAAAAGA CGTGGCTCAA AGTCTCTAAG CAGACTAAAG CTTTGATTGA TAAGCTCCAA
    AAGCTTGTGT CATCTGAGGG CAGATTTAAG AATCTCAGAG AAGCTCTGAA AAATTGTGAC
    CCACCCTGTG TCCCTTACCT GGGGATGTAC CTCACCGACC TGGCCTTCAT CGAGGAGGGG
    ACGCCCAATT ACACGGAAGA CGGCCTGGTC AACTTCTCCA AGATGAGGAT GATATCCCAT
    ATTATCCGAG AGATTCGCCA GTTTCAACAA ACTGCCTACA AAATAGAGCA CCAAGCAAAG
    GTAACGCAAT ATTTACTGGA CCAATCTTTT GTAATGGATG AAGAAAGCCT CTACGAGTCT
    TCTCTCCGAA TAGAACCAAA ACTCCCCACC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016877944.1
    CDS276..4088
    Translation

    Target ORF information:

    RefSeq Version XM_017022455.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022455.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGAGG ACCAGGGAGG GGACCAGGGA AGCAGAAATG
    AGCAGGTCCG ACGCCTCCTT ATATTGTGAT GATGTTGACA TTCGCTTCAG CAAAACCATG
    AACTCCTGCA AAGTGCTGCA GATCCGCTAC GCCAGTGTGG AGCGGCTGCT GGAGAGGCTG
    ACGGACCTGC GCTTCCTGAG CATCGACTTC CTCAACACCT TCCTGCACTC CTACCGCGTC
    TTCACCACCG CCATCGTGGT CCTGGACAAG CTCATTACCA TCTACAAGAA GCCTATCAGT
    GCCATTCCTG CCAGGTCGCT GGAGCTCCTG TTTGCCAGTG GCCAGAACAA TAAGCTCCTG
    TACGGTGAAC CCCCCAAGTC CCCGCGCGCC ACCCGCAAGT TCTCCTCGCC GCCACCTCTG
    TCCATCACCA AGACATCGTC ACCGAGCCGC CGGCGGAAGC TCTCCCTGAA CATCCCCATC
    ATCACTGGCG GCAAGGCCCT GGACCTGGCC GCCCTCAGCT GCAACTCCAA TGGCTACACC
    AGCATGTACT CGGCCATGTC ACCCTTCAGC AAGGCCACGC TGGACACCAG CAAGCTCTAT
    GTGTCCAGCA GCTTCACCAA CAAGATTCCA GATGAGGGCG ATACGACCCC TGAGAAGCCC
    GAAGACCCTT CAGCGCTCAG CAAGCAGAGC TCAGAAGTCT CCATGAGAGA GGAGTCAGAT
    ATTGATCAAA ACCAGAGTGA TGATGGTGAT ACTGAAACAT CACCAACTAA ATCTCCAACA
    ACACCCAAAT CAGTCAAAAA CAAAAATTCT TCAGAGTTCC CACTCTTTTC CTATAACAAT
    GGAGTCGTCA TGACCTCCTG TCGTGAACTG GACAATAACC GCAGTGCCTT GTCGGCCGCC
    TCTGCCTTTG CCATAGCAAC CGCCGGGGCC AACGAGGGCA CCCCAAACAA GGAGAAGTAC
    CGGAGGATGT CCTTAGCCAG TGCAGGGTTT CCCCCAGACC AGAGGAATGG AGACAAGGAG
    TTTGTGATCC GCAGAGCAGC CACCAATCGT GTCTTGAACG TGCTCCGCCA CTGGGTGTCC
    AAGCACTCTC AGGACTTTGA GACCAACGAT GAGCTCAAAT GCAAGGTGAT CGGCTTCCTG
    GAAGAAGTCA TGCACGACCC GGAGCTCCTG ACCCAGGAGC GGAAGGCTGC AGCCAACATC
    ATCAGGACTC TGACCCAGGA GGACCCAGGT GACAACCAGA TCACGCTGGA GGAGATCACG
    CAGATGGCTG AAGGCGTGAA GGCTGAGCCC TTTGAAAACC ACTCAGCCCT GGAGATCGCG
    GAGCAGCTGA CCCTGCTAGA TCACCTCGTC TTCAAGAAGA TTCCTTATGA GGAGTTCTTC
    GGACAAGGAT GGATGAAACT GGAAAAGAAT GAAAGGACCC CTTATATCAT GAAAACCACT
    AAGCACTTCA ATGACATCAG TAACTTGATT GCTTCAGAAA TCATCCGCAA TGAGGACATC
    AACGCCAGGG TGAGCGCCAT CGAGAAGTGG GTGGCCGTAG CTGACATATG CCGCTGCCTC
    CACAACTACA ATGCCGTACT GGAGATCACC TCGTCCATGA ACCGCAGTGC AATCTTCCGG
    CTCAAAAAGA CGTGGCTCAA AGTCTCTAAG CAGACTAAAG CTTTGATTGA TAAGCTCCAA
    AAGCTTGTGT CATCTGAGGG CAGATTTAAG AATCTCAGAG AAGCTCTGAA AAATTGTGAC
    CCACCCTGTG TCCCTTACCT GGGGATGTAC CTCACCGACC TGGCCTTCAT CGAGGAGGGG
    ACGCCCAATT ACACGGAAGA CGGCCTGGTC AACTTCTCCA AGATGAGGAT GATATCCCAT
    ATTATCCGAG AGATTCGCCA GTTTCAACAA ACTGCCTACA AAATAGAGCA CCAAGCAAAG
    GTAACGCAAT ATTTACTGGA CCAATCTTTT GTAATGGATG AAGAAAGCCT CTACGAGTCT
    TCTCTCCGAA TAGAACCAAA ACTCCCCACC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85583
    Clone ID Related Accession (Same CDS sequence) XM_017022456.2 , XM_017022456.1
    Accession Version XM_017022456.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3783bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ras-specific guanine nucleotide-releasing factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017022456.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TATATTGTGA TGATGTTGAC
    ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGCTA CGCCAGTGTG
    GAGCGGCTGC TGGAGAGGCT GACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC
    TTCCTGCACT CCTACCGCGT CTTCACCACC GCCATCGTGG TCCTGGACAA GCTCATTACC
    ATCTACAAGA AGCCTATCAG TGCCATTCCT GCCAGGTGGC TGAGGTCGCT GGAGCTCCTG
    TTTGCCAGTG GCCAGAACAA TAAGCTCCTG TACGGTGAAC CCCCCAAGTC CCCGCGCGCC
    ACCCGCAAGT TCTCCTCGCC GCCACCTCTG TCCATCACCA AGACATCGTC ACCGAGCCGC
    CGGCGGAAGC TCTCCCTGAA CATCCCCATC ATCACTGGCG GCAAGGCCCT GGACCTGGCC
    GCCCTCAGCT GCAACTCCAA TGGCTACACC AGCATGTACT CGGCCATGTC ACCCTTCAGC
    AAGGCCACGC TGGACACCAG CAAGCTCTAT GTGTCCAGCA GCTTCACCAA CAAGATTCCA
    GATGAGGGCG ATACGACCCC TGAGAAGCCC GAAGACCCTT CAGCGCTCAG CAAGCAGAGC
    TCAGAAGTCT CCATGAGAGA GGAGTCAGAT ATTGATCAAA ACCAGAGTGA TGATGGTGAT
    ACTGAAACAT CACCAACTAA ATCTCCAACA ACACCCAAAT CAGTCAAAAA CAAAAATTCT
    TCAGAGTTCC CACTCTTTTC CTATAACAAT GGAGTCGTCA TGACCTCCTG TCGTGAACTG
    GACAATAACC GCAGTGCCTT GTCGGCCGCC TCTGCCTTTG CCATAGCAAC CGCCGGGGCC
    AACGAGGGCA CCCCAAACAA GGAGAAGTAC CGGAGGATGT CCTTAGCCAG TGCAGGGTTT
    CCCCCAGACC AGAGGAATGG AGACAAGGAG TTTGTGATCC GCAGAGCAGC CACCAATCGT
    GTCTTGAACG TGCTCCGCCA CTGGGTGTCC AAGCACTCTC AGGACTTTGA GACCAACGAT
    GAGCTCAAAT GCAAGGTGAT CGGCTTCCTG GAAGAAGTCA TGCACGACCC GGAGCTCCTG
    ACCCAGGAGC GGAAGGCTGC AGCCAACATC ATCAGGACTC TGACCCAGGA GGACCCAGGT
    GACAACCAGA TCACGCTGGA GGAGATCACG CAGATGGCTG AAGGCGTGAA GGCTGAGCCC
    TTTGAAAACC ACTCAGCCCT GGAGATCGCG GAGCAGCTGA CCCTGCTAGA TCACCTCGTC
    TTCAAGAAGA TTCCTTATGA GGAGTTCTTC GGACAAGGAT GGATGAAACT GGAAAAGAAT
    GAAAGGACCC CTTATATCAT GAAAACCACT AAGCACTTCA ATGACATCAG TAACTTGATT
    GCTTCAGAAA TCATCCGCAA TGAGGACATC AACGCCAGGG TGAGCGCCAT CGAGAAGTGG
    GTGGCCGTAG CTGACATATG CCGCTGCCTC CACAACTACA ATGCCGTACT GGAGATCACC
    TCGTCCATGA ACCGCAGTGC AATCTTCCGG CTCAAAAAGA CGTGGCTCAA AGTCTCTAAG
    CAGACTAAAG CTTTGATTGA TAAGCTCCAA AAGCTTGTGT CATCTGAGGG CAGATTTAAG
    AATCTCAGAG AAGCTCTGAA AAATTGTGAC CCACCCTGTG TCCCTTACCT GGGGATGTAC
    CTCACCGACC TGGCCTTCAT CGAGGAGGGG ACGCCCAATT ACACGGAAGA CGGCCTGGTC
    AACTTCTCCA AGATGAGGAT GATATCCCAT ATTATCCGAG AGATTCGCCA GTTTCAACAA
    ACTGCCTACA AAATAGAGCA CCAAGCAAAG GTAACGCAAT ATTTACTGGA CCAATCTTTT
    GTAATGGATG AAGAAAGCCT CTACGAGTCT TCTCTCCGAA TAGAACCAAA ACTCCCCACC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016877945.1
    CDS275..4057
    Translation

    Target ORF information:

    RefSeq Version XM_017022456.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022456.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TATATTGTGA TGATGTTGAC
    ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGCTA CGCCAGTGTG
    GAGCGGCTGC TGGAGAGGCT GACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC
    TTCCTGCACT CCTACCGCGT CTTCACCACC GCCATCGTGG TCCTGGACAA GCTCATTACC
    ATCTACAAGA AGCCTATCAG TGCCATTCCT GCCAGGTGGC TGAGGTCGCT GGAGCTCCTG
    TTTGCCAGTG GCCAGAACAA TAAGCTCCTG TACGGTGAAC CCCCCAAGTC CCCGCGCGCC
    ACCCGCAAGT TCTCCTCGCC GCCACCTCTG TCCATCACCA AGACATCGTC ACCGAGCCGC
    CGGCGGAAGC TCTCCCTGAA CATCCCCATC ATCACTGGCG GCAAGGCCCT GGACCTGGCC
    GCCCTCAGCT GCAACTCCAA TGGCTACACC AGCATGTACT CGGCCATGTC ACCCTTCAGC
    AAGGCCACGC TGGACACCAG CAAGCTCTAT GTGTCCAGCA GCTTCACCAA CAAGATTCCA
    GATGAGGGCG ATACGACCCC TGAGAAGCCC GAAGACCCTT CAGCGCTCAG CAAGCAGAGC
    TCAGAAGTCT CCATGAGAGA GGAGTCAGAT ATTGATCAAA ACCAGAGTGA TGATGGTGAT
    ACTGAAACAT CACCAACTAA ATCTCCAACA ACACCCAAAT CAGTCAAAAA CAAAAATTCT
    TCAGAGTTCC CACTCTTTTC CTATAACAAT GGAGTCGTCA TGACCTCCTG TCGTGAACTG
    GACAATAACC GCAGTGCCTT GTCGGCCGCC TCTGCCTTTG CCATAGCAAC CGCCGGGGCC
    AACGAGGGCA CCCCAAACAA GGAGAAGTAC CGGAGGATGT CCTTAGCCAG TGCAGGGTTT
    CCCCCAGACC AGAGGAATGG AGACAAGGAG TTTGTGATCC GCAGAGCAGC CACCAATCGT
    GTCTTGAACG TGCTCCGCCA CTGGGTGTCC AAGCACTCTC AGGACTTTGA GACCAACGAT
    GAGCTCAAAT GCAAGGTGAT CGGCTTCCTG GAAGAAGTCA TGCACGACCC GGAGCTCCTG
    ACCCAGGAGC GGAAGGCTGC AGCCAACATC ATCAGGACTC TGACCCAGGA GGACCCAGGT
    GACAACCAGA TCACGCTGGA GGAGATCACG CAGATGGCTG AAGGCGTGAA GGCTGAGCCC
    TTTGAAAACC ACTCAGCCCT GGAGATCGCG GAGCAGCTGA CCCTGCTAGA TCACCTCGTC
    TTCAAGAAGA TTCCTTATGA GGAGTTCTTC GGACAAGGAT GGATGAAACT GGAAAAGAAT
    GAAAGGACCC CTTATATCAT GAAAACCACT AAGCACTTCA ATGACATCAG TAACTTGATT
    GCTTCAGAAA TCATCCGCAA TGAGGACATC AACGCCAGGG TGAGCGCCAT CGAGAAGTGG
    GTGGCCGTAG CTGACATATG CCGCTGCCTC CACAACTACA ATGCCGTACT GGAGATCACC
    TCGTCCATGA ACCGCAGTGC AATCTTCCGG CTCAAAAAGA CGTGGCTCAA AGTCTCTAAG
    CAGACTAAAG CTTTGATTGA TAAGCTCCAA AAGCTTGTGT CATCTGAGGG CAGATTTAAG
    AATCTCAGAG AAGCTCTGAA AAATTGTGAC CCACCCTGTG TCCCTTACCT GGGGATGTAC
    CTCACCGACC TGGCCTTCAT CGAGGAGGGG ACGCCCAATT ACACGGAAGA CGGCCTGGTC
    AACTTCTCCA AGATGAGGAT GATATCCCAT ATTATCCGAG AGATTCGCCA GTTTCAACAA
    ACTGCCTACA AAATAGAGCA CCAAGCAAAG GTAACGCAAT ATTTACTGGA CCAATCTTTT
    GTAATGGATG AAGAAAGCCT CTACGAGTCT TCTCTCCGAA TAGAACCAAA ACTCCCCACC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85583
    Clone ID Related Accession (Same CDS sequence) XM_017022456.2 , XM_017022456.1
    Accession Version XM_017022456.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3783bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ras-specific guanine nucleotide-releasing factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TATATTGTGA TGATGTTGAC
    ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGCTA CGCCAGTGTG
    GAGCGGCTGC TGGAGAGGCT GACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC
    TTCCTGCACT CCTACCGCGT CTTCACCACC GCCATCGTGG TCCTGGACAA GCTCATTACC
    ATCTACAAGA AGCCTATCAG TGCCATTCCT GCCAGGTGGC TGAGGTCGCT GGAGCTCCTG
    TTTGCCAGTG GCCAGAACAA TAAGCTCCTG TACGGTGAAC CCCCCAAGTC CCCGCGCGCC
    ACCCGCAAGT TCTCCTCGCC GCCACCTCTG TCCATCACCA AGACATCGTC ACCGAGCCGC
    CGGCGGAAGC TCTCCCTGAA CATCCCCATC ATCACTGGCG GCAAGGCCCT GGACCTGGCC
    GCCCTCAGCT GCAACTCCAA TGGCTACACC AGCATGTACT CGGCCATGTC ACCCTTCAGC
    AAGGCCACGC TGGACACCAG CAAGCTCTAT GTGTCCAGCA GCTTCACCAA CAAGATTCCA
    GATGAGGGCG ATACGACCCC TGAGAAGCCC GAAGACCCTT CAGCGCTCAG CAAGCAGAGC
    TCAGAAGTCT CCATGAGAGA GGAGTCAGAT ATTGATCAAA ACCAGAGTGA TGATGGTGAT
    ACTGAAACAT CACCAACTAA ATCTCCAACA ACACCCAAAT CAGTCAAAAA CAAAAATTCT
    TCAGAGTTCC CACTCTTTTC CTATAACAAT GGAGTCGTCA TGACCTCCTG TCGTGAACTG
    GACAATAACC GCAGTGCCTT GTCGGCCGCC TCTGCCTTTG CCATAGCAAC CGCCGGGGCC
    AACGAGGGCA CCCCAAACAA GGAGAAGTAC CGGAGGATGT CCTTAGCCAG TGCAGGGTTT
    CCCCCAGACC AGAGGAATGG AGACAAGGAG TTTGTGATCC GCAGAGCAGC CACCAATCGT
    GTCTTGAACG TGCTCCGCCA CTGGGTGTCC AAGCACTCTC AGGACTTTGA GACCAACGAT
    GAGCTCAAAT GCAAGGTGAT CGGCTTCCTG GAAGAAGTCA TGCACGACCC GGAGCTCCTG
    ACCCAGGAGC GGAAGGCTGC AGCCAACATC ATCAGGACTC TGACCCAGGA GGACCCAGGT
    GACAACCAGA TCACGCTGGA GGAGATCACG CAGATGGCTG AAGGCGTGAA GGCTGAGCCC
    TTTGAAAACC ACTCAGCCCT GGAGATCGCG GAGCAGCTGA CCCTGCTAGA TCACCTCGTC
    TTCAAGAAGA TTCCTTATGA GGAGTTCTTC GGACAAGGAT GGATGAAACT GGAAAAGAAT
    GAAAGGACCC CTTATATCAT GAAAACCACT AAGCACTTCA ATGACATCAG TAACTTGATT
    GCTTCAGAAA TCATCCGCAA TGAGGACATC AACGCCAGGG TGAGCGCCAT CGAGAAGTGG
    GTGGCCGTAG CTGACATATG CCGCTGCCTC CACAACTACA ATGCCGTACT GGAGATCACC
    TCGTCCATGA ACCGCAGTGC AATCTTCCGG CTCAAAAAGA CGTGGCTCAA AGTCTCTAAG
    CAGACTAAAG CTTTGATTGA TAAGCTCCAA AAGCTTGTGT CATCTGAGGG CAGATTTAAG
    AATCTCAGAG AAGCTCTGAA AAATTGTGAC CCACCCTGTG TCCCTTACCT GGGGATGTAC
    CTCACCGACC TGGCCTTCAT CGAGGAGGGG ACGCCCAATT ACACGGAAGA CGGCCTGGTC
    AACTTCTCCA AGATGAGGAT GATATCCCAT ATTATCCGAG AGATTCGCCA GTTTCAACAA
    ACTGCCTACA AAATAGAGCA CCAAGCAAAG GTAACGCAAT ATTTACTGGA CCAATCTTTT
    GTAATGGATG AAGAAAGCCT CTACGAGTCT TCTCTCCGAA TAGAACCAAA ACTCCCCACC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016877945.1
    CDS276..4058
    Translation

    Target ORF information:

    RefSeq Version XM_017022456.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022456.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TATATTGTGA TGATGTTGAC
    ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGCTA CGCCAGTGTG
    GAGCGGCTGC TGGAGAGGCT GACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC
    TTCCTGCACT CCTACCGCGT CTTCACCACC GCCATCGTGG TCCTGGACAA GCTCATTACC
    ATCTACAAGA AGCCTATCAG TGCCATTCCT GCCAGGTGGC TGAGGTCGCT GGAGCTCCTG
    TTTGCCAGTG GCCAGAACAA TAAGCTCCTG TACGGTGAAC CCCCCAAGTC CCCGCGCGCC
    ACCCGCAAGT TCTCCTCGCC GCCACCTCTG TCCATCACCA AGACATCGTC ACCGAGCCGC
    CGGCGGAAGC TCTCCCTGAA CATCCCCATC ATCACTGGCG GCAAGGCCCT GGACCTGGCC
    GCCCTCAGCT GCAACTCCAA TGGCTACACC AGCATGTACT CGGCCATGTC ACCCTTCAGC
    AAGGCCACGC TGGACACCAG CAAGCTCTAT GTGTCCAGCA GCTTCACCAA CAAGATTCCA
    GATGAGGGCG ATACGACCCC TGAGAAGCCC GAAGACCCTT CAGCGCTCAG CAAGCAGAGC
    TCAGAAGTCT CCATGAGAGA GGAGTCAGAT ATTGATCAAA ACCAGAGTGA TGATGGTGAT
    ACTGAAACAT CACCAACTAA ATCTCCAACA ACACCCAAAT CAGTCAAAAA CAAAAATTCT
    TCAGAGTTCC CACTCTTTTC CTATAACAAT GGAGTCGTCA TGACCTCCTG TCGTGAACTG
    GACAATAACC GCAGTGCCTT GTCGGCCGCC TCTGCCTTTG CCATAGCAAC CGCCGGGGCC
    AACGAGGGCA CCCCAAACAA GGAGAAGTAC CGGAGGATGT CCTTAGCCAG TGCAGGGTTT
    CCCCCAGACC AGAGGAATGG AGACAAGGAG TTTGTGATCC GCAGAGCAGC CACCAATCGT
    GTCTTGAACG TGCTCCGCCA CTGGGTGTCC AAGCACTCTC AGGACTTTGA GACCAACGAT
    GAGCTCAAAT GCAAGGTGAT CGGCTTCCTG GAAGAAGTCA TGCACGACCC GGAGCTCCTG
    ACCCAGGAGC GGAAGGCTGC AGCCAACATC ATCAGGACTC TGACCCAGGA GGACCCAGGT
    GACAACCAGA TCACGCTGGA GGAGATCACG CAGATGGCTG AAGGCGTGAA GGCTGAGCCC
    TTTGAAAACC ACTCAGCCCT GGAGATCGCG GAGCAGCTGA CCCTGCTAGA TCACCTCGTC
    TTCAAGAAGA TTCCTTATGA GGAGTTCTTC GGACAAGGAT GGATGAAACT GGAAAAGAAT
    GAAAGGACCC CTTATATCAT GAAAACCACT AAGCACTTCA ATGACATCAG TAACTTGATT
    GCTTCAGAAA TCATCCGCAA TGAGGACATC AACGCCAGGG TGAGCGCCAT CGAGAAGTGG
    GTGGCCGTAG CTGACATATG CCGCTGCCTC CACAACTACA ATGCCGTACT GGAGATCACC
    TCGTCCATGA ACCGCAGTGC AATCTTCCGG CTCAAAAAGA CGTGGCTCAA AGTCTCTAAG
    CAGACTAAAG CTTTGATTGA TAAGCTCCAA AAGCTTGTGT CATCTGAGGG CAGATTTAAG
    AATCTCAGAG AAGCTCTGAA AAATTGTGAC CCACCCTGTG TCCCTTACCT GGGGATGTAC
    CTCACCGACC TGGCCTTCAT CGAGGAGGGG ACGCCCAATT ACACGGAAGA CGGCCTGGTC
    AACTTCTCCA AGATGAGGAT GATATCCCAT ATTATCCGAG AGATTCGCCA GTTTCAACAA
    ACTGCCTACA AAATAGAGCA CCAAGCAAAG GTAACGCAAT ATTTACTGGA CCAATCTTTT
    GTAATGGATG AAGAAAGCCT CTACGAGTCT TCTCTCCGAA TAGAACCAAA ACTCCCCACC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85584
    Clone ID Related Accession (Same CDS sequence) XM_017022457.2 , XM_017022457.1
    Accession Version XM_017022457.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3747bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ras-specific guanine nucleotide-releasing factor 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017022457.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TATATTGTGA TGATGTTGAC
    ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGCTA CGCCAGTGTG
    GAGCGGCTGC TGGAGAGGCT GACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC
    TTCCTGCACT CCTACCGCGT CTTCACCACC GCCATCGTGG TCCTGGACAA GCTCATTACC
    ATCTACAAGA AGCCTATCAG TGCCATTCCT GCCAGGTGGC TGAGGTCGCT GGAGCTCCTG
    TTTGCCAGTG GCCAGAACAA TAAGCTCCTG TACGGTGAAC CCCCCAAGTC CCCGCGCGCC
    ACCCGCAAGT TCTCCTCGCC GCCACCTCTG TCCATCACCA AGACATCGTC ACCGAGCCGC
    CGGCGGAAGC TCTCCCTGAA CATCCCCATC ATCACTGGCG GCAAGGCCCT GGACCTGGCC
    GCCCTCAGCT GCAACTCCAA TGGCTACACC AGCATGTACT CGGCCATGTC ACCCTTCAGC
    AAGGCCACGC TGGACACCAG CAAGCTCTAT GTGTCCAGCA GCTTCACCAA CAAGATTCCA
    GATGAGGGCG ATACGACCCC TGAGAAGCCC GAAGACCCTT CAGCGCTCAG CAAGCAGAGC
    TCAGAAGTCT CCATGAGAGA GGAGTCAGAT ATTGATCAAA ACCAGAGTGA TGATGGTGAT
    ACTGAAACAT CACCAACTAA ATCTCCAACA ACACCCAAAT CAGTCAAAAA CAAAAATTCT
    TCAGAGTTCC CACTCTTTTC CTATAACAAT GGAGTCGTCA TGACCTCCTG TCGTGAACTG
    GACAATAACC GCAGTGCCTT GTCGGCCGCC TCTGCCTTTG CCATAGCAAC CGCCGGGGCC
    AACGAGGGCA CCCCAAACAA GGAGAAGTAC CGGAGGATGT CCTTAGCCAG TGCAGGGTTT
    CCCCCAGACC AGAGGAATGG AGACAAGGAG TTTGTGATCC GCAGAGCAGC CACCAATCGT
    GTCTTGAACG TGCTCCGCCA CTGGGTGTCC AAGCACTCTC AGGACTTTGA GACCAACGAT
    GAGCTCAAAT GCAAGGTGAT CGGCTTCCTG GAAGAAGTCA TGCACGACCC GGAGCTCCTG
    ACCCAGGAGC GGAAGGCTGC AGCCAACATC ATCAGGACTC TGACCCAGGA GGACCCAGGT
    GACAACCAGA TCACGCTGGA GGAGATCACG CAGATGGCTG AAGGCGTGAA GGCTGAGCCC
    TTTGAAAACC ACTCAGCCCT GGAGATCGCG GAGCAGCTGA CCCTGCTAGA TCACCTCGTC
    TTCAAGAAGA TTCCTTATGA GGAGTTCTTC GGACAAGGAT GGATGAAACT GGAAAAGAAT
    GAAAGGACCC CTTATATCAT GAAAACCACT AAGCACTTCA ATGACATCAG TAACTTGATT
    GCTTCAGAAA TCATCCGCAA TGAGGACATC AACGCCAGGG TGAGCGCCAT CGAGAAGTGG
    GTGGCCGTAG CTGACATATG CCGCTGCCTC CACAACTACA ATGCCGTACT GGAGATCACC
    TCGTCCATGA ACCGCAGTGC AATCTTCCGG CTCAAAAAGA CGTGGCTCAA AGTCTCTAAG
    CAGACTAAAG CTTTGATTGA TAAGCTCCAA AAGCTTGTGT CATCTGAGGG CAGATTTAAG
    AATCTCAGAG AAGCTCTGAA AAAACAGATG TTGCACCCAT CTGTGCTGTC CAGCCGTCTC
    TCTTCTGCCT GGGCTCCCGA GAGCCCCTGT GGACTGTGCT TGTGGGGAGC TGCCCCCTCC
    GTGCATTCAC CAACTTGTCC GTCCGTCCGC CCCCGGGGCA CCACTCCATC CACCTCCTCA
    CATGGCTGGC TTCCTCGTCT GCCGCCGCCA CCACCGCTGC CTCCACTGCC TCTGGGGCCC
    CCCATTCTGT CTGAGTCCCC ACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016877946.1
    CDS275..4021
    Translation

    Target ORF information:

    RefSeq Version XM_017022457.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022457.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TATATTGTGA TGATGTTGAC
    ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGCTA CGCCAGTGTG
    GAGCGGCTGC TGGAGAGGCT GACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC
    TTCCTGCACT CCTACCGCGT CTTCACCACC GCCATCGTGG TCCTGGACAA GCTCATTACC
    ATCTACAAGA AGCCTATCAG TGCCATTCCT GCCAGGTGGC TGAGGTCGCT GGAGCTCCTG
    TTTGCCAGTG GCCAGAACAA TAAGCTCCTG TACGGTGAAC CCCCCAAGTC CCCGCGCGCC
    ACCCGCAAGT TCTCCTCGCC GCCACCTCTG TCCATCACCA AGACATCGTC ACCGAGCCGC
    CGGCGGAAGC TCTCCCTGAA CATCCCCATC ATCACTGGCG GCAAGGCCCT GGACCTGGCC
    GCCCTCAGCT GCAACTCCAA TGGCTACACC AGCATGTACT CGGCCATGTC ACCCTTCAGC
    AAGGCCACGC TGGACACCAG CAAGCTCTAT GTGTCCAGCA GCTTCACCAA CAAGATTCCA
    GATGAGGGCG ATACGACCCC TGAGAAGCCC GAAGACCCTT CAGCGCTCAG CAAGCAGAGC
    TCAGAAGTCT CCATGAGAGA GGAGTCAGAT ATTGATCAAA ACCAGAGTGA TGATGGTGAT
    ACTGAAACAT CACCAACTAA ATCTCCAACA ACACCCAAAT CAGTCAAAAA CAAAAATTCT
    TCAGAGTTCC CACTCTTTTC CTATAACAAT GGAGTCGTCA TGACCTCCTG TCGTGAACTG
    GACAATAACC GCAGTGCCTT GTCGGCCGCC TCTGCCTTTG CCATAGCAAC CGCCGGGGCC
    AACGAGGGCA CCCCAAACAA GGAGAAGTAC CGGAGGATGT CCTTAGCCAG TGCAGGGTTT
    CCCCCAGACC AGAGGAATGG AGACAAGGAG TTTGTGATCC GCAGAGCAGC CACCAATCGT
    GTCTTGAACG TGCTCCGCCA CTGGGTGTCC AAGCACTCTC AGGACTTTGA GACCAACGAT
    GAGCTCAAAT GCAAGGTGAT CGGCTTCCTG GAAGAAGTCA TGCACGACCC GGAGCTCCTG
    ACCCAGGAGC GGAAGGCTGC AGCCAACATC ATCAGGACTC TGACCCAGGA GGACCCAGGT
    GACAACCAGA TCACGCTGGA GGAGATCACG CAGATGGCTG AAGGCGTGAA GGCTGAGCCC
    TTTGAAAACC ACTCAGCCCT GGAGATCGCG GAGCAGCTGA CCCTGCTAGA TCACCTCGTC
    TTCAAGAAGA TTCCTTATGA GGAGTTCTTC GGACAAGGAT GGATGAAACT GGAAAAGAAT
    GAAAGGACCC CTTATATCAT GAAAACCACT AAGCACTTCA ATGACATCAG TAACTTGATT
    GCTTCAGAAA TCATCCGCAA TGAGGACATC AACGCCAGGG TGAGCGCCAT CGAGAAGTGG
    GTGGCCGTAG CTGACATATG CCGCTGCCTC CACAACTACA ATGCCGTACT GGAGATCACC
    TCGTCCATGA ACCGCAGTGC AATCTTCCGG CTCAAAAAGA CGTGGCTCAA AGTCTCTAAG
    CAGACTAAAG CTTTGATTGA TAAGCTCCAA AAGCTTGTGT CATCTGAGGG CAGATTTAAG
    AATCTCAGAG AAGCTCTGAA AAAACAGATG TTGCACCCAT CTGTGCTGTC CAGCCGTCTC
    TCTTCTGCCT GGGCTCCCGA GAGCCCCTGT GGACTGTGCT TGTGGGGAGC TGCCCCCTCC
    GTGCATTCAC CAACTTGTCC GTCCGTCCGC CCCCGGGGCA CCACTCCATC CACCTCCTCA
    CATGGCTGGC TTCCTCGTCT GCCGCCGCCA CCACCGCTGC CTCCACTGCC TCTGGGGCCC
    CCCATTCTGT CTGAGTCCCC ACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85584
    Clone ID Related Accession (Same CDS sequence) XM_017022457.2 , XM_017022457.1
    Accession Version XM_017022457.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3747bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ras-specific guanine nucleotide-releasing factor 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TATATTGTGA TGATGTTGAC
    ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGCTA CGCCAGTGTG
    GAGCGGCTGC TGGAGAGGCT GACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC
    TTCCTGCACT CCTACCGCGT CTTCACCACC GCCATCGTGG TCCTGGACAA GCTCATTACC
    ATCTACAAGA AGCCTATCAG TGCCATTCCT GCCAGGTGGC TGAGGTCGCT GGAGCTCCTG
    TTTGCCAGTG GCCAGAACAA TAAGCTCCTG TACGGTGAAC CCCCCAAGTC CCCGCGCGCC
    ACCCGCAAGT TCTCCTCGCC GCCACCTCTG TCCATCACCA AGACATCGTC ACCGAGCCGC
    CGGCGGAAGC TCTCCCTGAA CATCCCCATC ATCACTGGCG GCAAGGCCCT GGACCTGGCC
    GCCCTCAGCT GCAACTCCAA TGGCTACACC AGCATGTACT CGGCCATGTC ACCCTTCAGC
    AAGGCCACGC TGGACACCAG CAAGCTCTAT GTGTCCAGCA GCTTCACCAA CAAGATTCCA
    GATGAGGGCG ATACGACCCC TGAGAAGCCC GAAGACCCTT CAGCGCTCAG CAAGCAGAGC
    TCAGAAGTCT CCATGAGAGA GGAGTCAGAT ATTGATCAAA ACCAGAGTGA TGATGGTGAT
    ACTGAAACAT CACCAACTAA ATCTCCAACA ACACCCAAAT CAGTCAAAAA CAAAAATTCT
    TCAGAGTTCC CACTCTTTTC CTATAACAAT GGAGTCGTCA TGACCTCCTG TCGTGAACTG
    GACAATAACC GCAGTGCCTT GTCGGCCGCC TCTGCCTTTG CCATAGCAAC CGCCGGGGCC
    AACGAGGGCA CCCCAAACAA GGAGAAGTAC CGGAGGATGT CCTTAGCCAG TGCAGGGTTT
    CCCCCAGACC AGAGGAATGG AGACAAGGAG TTTGTGATCC GCAGAGCAGC CACCAATCGT
    GTCTTGAACG TGCTCCGCCA CTGGGTGTCC AAGCACTCTC AGGACTTTGA GACCAACGAT
    GAGCTCAAAT GCAAGGTGAT CGGCTTCCTG GAAGAAGTCA TGCACGACCC GGAGCTCCTG
    ACCCAGGAGC GGAAGGCTGC AGCCAACATC ATCAGGACTC TGACCCAGGA GGACCCAGGT
    GACAACCAGA TCACGCTGGA GGAGATCACG CAGATGGCTG AAGGCGTGAA GGCTGAGCCC
    TTTGAAAACC ACTCAGCCCT GGAGATCGCG GAGCAGCTGA CCCTGCTAGA TCACCTCGTC
    TTCAAGAAGA TTCCTTATGA GGAGTTCTTC GGACAAGGAT GGATGAAACT GGAAAAGAAT
    GAAAGGACCC CTTATATCAT GAAAACCACT AAGCACTTCA ATGACATCAG TAACTTGATT
    GCTTCAGAAA TCATCCGCAA TGAGGACATC AACGCCAGGG TGAGCGCCAT CGAGAAGTGG
    GTGGCCGTAG CTGACATATG CCGCTGCCTC CACAACTACA ATGCCGTACT GGAGATCACC
    TCGTCCATGA ACCGCAGTGC AATCTTCCGG CTCAAAAAGA CGTGGCTCAA AGTCTCTAAG
    CAGACTAAAG CTTTGATTGA TAAGCTCCAA AAGCTTGTGT CATCTGAGGG CAGATTTAAG
    AATCTCAGAG AAGCTCTGAA AAAACAGATG TTGCACCCAT CTGTGCTGTC CAGCCGTCTC
    TCTTCTGCCT GGGCTCCCGA GAGCCCCTGT GGACTGTGCT TGTGGGGAGC TGCCCCCTCC
    GTGCATTCAC CAACTTGTCC GTCCGTCCGC CCCCGGGGCA CCACTCCATC CACCTCCTCA
    CATGGCTGGC TTCCTCGTCT GCCGCCGCCA CCACCGCTGC CTCCACTGCC TCTGGGGCCC
    CCCATTCTGT CTGAGTCCCC ACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016877946.1
    CDS276..4022
    Translation

    Target ORF information:

    RefSeq Version XM_017022457.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022457.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TATATTGTGA TGATGTTGAC
    ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGCTA CGCCAGTGTG
    GAGCGGCTGC TGGAGAGGCT GACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC
    TTCCTGCACT CCTACCGCGT CTTCACCACC GCCATCGTGG TCCTGGACAA GCTCATTACC
    ATCTACAAGA AGCCTATCAG TGCCATTCCT GCCAGGTGGC TGAGGTCGCT GGAGCTCCTG
    TTTGCCAGTG GCCAGAACAA TAAGCTCCTG TACGGTGAAC CCCCCAAGTC CCCGCGCGCC
    ACCCGCAAGT TCTCCTCGCC GCCACCTCTG TCCATCACCA AGACATCGTC ACCGAGCCGC
    CGGCGGAAGC TCTCCCTGAA CATCCCCATC ATCACTGGCG GCAAGGCCCT GGACCTGGCC
    GCCCTCAGCT GCAACTCCAA TGGCTACACC AGCATGTACT CGGCCATGTC ACCCTTCAGC
    AAGGCCACGC TGGACACCAG CAAGCTCTAT GTGTCCAGCA GCTTCACCAA CAAGATTCCA
    GATGAGGGCG ATACGACCCC TGAGAAGCCC GAAGACCCTT CAGCGCTCAG CAAGCAGAGC
    TCAGAAGTCT CCATGAGAGA GGAGTCAGAT ATTGATCAAA ACCAGAGTGA TGATGGTGAT
    ACTGAAACAT CACCAACTAA ATCTCCAACA ACACCCAAAT CAGTCAAAAA CAAAAATTCT
    TCAGAGTTCC CACTCTTTTC CTATAACAAT GGAGTCGTCA TGACCTCCTG TCGTGAACTG
    GACAATAACC GCAGTGCCTT GTCGGCCGCC TCTGCCTTTG CCATAGCAAC CGCCGGGGCC
    AACGAGGGCA CCCCAAACAA GGAGAAGTAC CGGAGGATGT CCTTAGCCAG TGCAGGGTTT
    CCCCCAGACC AGAGGAATGG AGACAAGGAG TTTGTGATCC GCAGAGCAGC CACCAATCGT
    GTCTTGAACG TGCTCCGCCA CTGGGTGTCC AAGCACTCTC AGGACTTTGA GACCAACGAT
    GAGCTCAAAT GCAAGGTGAT CGGCTTCCTG GAAGAAGTCA TGCACGACCC GGAGCTCCTG
    ACCCAGGAGC GGAAGGCTGC AGCCAACATC ATCAGGACTC TGACCCAGGA GGACCCAGGT
    GACAACCAGA TCACGCTGGA GGAGATCACG CAGATGGCTG AAGGCGTGAA GGCTGAGCCC
    TTTGAAAACC ACTCAGCCCT GGAGATCGCG GAGCAGCTGA CCCTGCTAGA TCACCTCGTC
    TTCAAGAAGA TTCCTTATGA GGAGTTCTTC GGACAAGGAT GGATGAAACT GGAAAAGAAT
    GAAAGGACCC CTTATATCAT GAAAACCACT AAGCACTTCA ATGACATCAG TAACTTGATT
    GCTTCAGAAA TCATCCGCAA TGAGGACATC AACGCCAGGG TGAGCGCCAT CGAGAAGTGG
    GTGGCCGTAG CTGACATATG CCGCTGCCTC CACAACTACA ATGCCGTACT GGAGATCACC
    TCGTCCATGA ACCGCAGTGC AATCTTCCGG CTCAAAAAGA CGTGGCTCAA AGTCTCTAAG
    CAGACTAAAG CTTTGATTGA TAAGCTCCAA AAGCTTGTGT CATCTGAGGG CAGATTTAAG
    AATCTCAGAG AAGCTCTGAA AAAACAGATG TTGCACCCAT CTGTGCTGTC CAGCCGTCTC
    TCTTCTGCCT GGGCTCCCGA GAGCCCCTGT GGACTGTGCT TGTGGGGAGC TGCCCCCTCC
    GTGCATTCAC CAACTTGTCC GTCCGTCCGC CCCCGGGGCA CCACTCCATC CACCTCCTCA
    CATGGCTGGC TTCCTCGTCT GCCGCCGCCA CCACCGCTGC CTCCACTGCC TCTGGGGCCC
    CCCATTCTGT CTGAGTCCCC ACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85585
    Clone ID Related Accession (Same CDS sequence) XM_017022458.2 , XM_017022458.1
    Accession Version XM_017022458.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3738bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ras-specific guanine nucleotide-releasing factor 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017022458.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TATATTGTGA TGATGTTGAC
    ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGCTA CGCCAGTGTG
    GAGCGGCTGC TGGAGAGGCT GACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC
    TTCCTGCACT CCTACCGCGT CTTCACCACC GCCATCGTGG TCCTGGACAA GCTCATTACC
    ATCTACAAGA AGCCTATCAG TGCCATTCCT GCCAGGTCGC TGGAGCTCCT GTTTGCCAGT
    GGCCAGAACA ATAAGCTCCT GTACGGTGAA CCCCCCAAGT CCCCGCGCGC CACCCGCAAG
    TTCTCCTCGC CGCCACCTCT GTCCATCACC AAGACATCGT CACCGAGCCG CCGGCGGAAG
    CTCTCCCTGA ACATCCCCAT CATCACTGGC GGCAAGGCCC TGGACCTGGC CGCCCTCAGC
    TGCAACTCCA ATGGCTACAC CAGCATGTAC TCGGCCATGT CACCCTTCAG CAAGGCCACG
    CTGGACACCA GCAAGCTCTA TGTGTCCAGC AGCTTCACCA ACAAGATTCC AGATGAGGGC
    GATACGACCC CTGAGAAGCC CGAAGACCCT TCAGCGCTCA GCAAGCAGAG CTCAGAAGTC
    TCCATGAGAG AGGAGTCAGA TATTGATCAA AACCAGAGTG ATGATGGTGA TACTGAAACA
    TCACCAACTA AATCTCCAAC AACACCCAAA TCAGTCAAAA ACAAAAATTC TTCAGAGTTC
    CCACTCTTTT CCTATAACAA TGGAGTCGTC ATGACCTCCT GTCGTGAACT GGACAATAAC
    CGCAGTGCCT TGTCGGCCGC CTCTGCCTTT GCCATAGCAA CCGCCGGGGC CAACGAGGGC
    ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTAGCCA GTGCAGGGTT TCCCCCAGAC
    CAGAGGAATG GAGACAAGGA GTTTGTGATC CGCAGAGCAG CCACCAATCG TGTCTTGAAC
    GTGCTCCGCC ACTGGGTGTC CAAGCACTCT CAGGACTTTG AGACCAACGA TGAGCTCAAA
    TGCAAGGTGA TCGGCTTCCT GGAAGAAGTC ATGCACGACC CGGAGCTCCT GACCCAGGAG
    CGGAAGGCTG CAGCCAACAT CATCAGGACT CTGACCCAGG AGGACCCAGG TGACAACCAG
    ATCACGCTGG AGGAGATCAC GCAGATGGCT GAAGGCGTGA AGGCTGAGCC CTTTGAAAAC
    CACTCAGCCC TGGAGATCGC GGAGCAGCTG ACCCTGCTAG ATCACCTCGT CTTCAAGAAG
    ATTCCTTATG AGGAGTTCTT CGGACAAGGA TGGATGAAAC TGGAAAAGAA TGAAAGGACC
    CCTTATATCA TGAAAACCAC TAAGCACTTC AATGACATCA GTAACTTGAT TGCTTCAGAA
    ATCATCCGCA ATGAGGACAT CAACGCCAGG GTGAGCGCCA TCGAGAAGTG GGTGGCCGTA
    GCTGACATAT GCCGCTGCCT CCACAACTAC AATGCCGTAC TGGAGATCAC CTCGTCCATG
    AACCGCAGTG CAATCTTCCG GCTCAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGACTAAA
    GCTTTGATTG ATAAGCTCCA AAAGCTTGTG TCATCTGAGG GCAGATTTAA GAATCTCAGA
    GAAGCTCTGA AAAAACAGAT GTTGCACCCA TCTGTGCTGT CCAGCCGTCT CTCTTCTGCC
    TGGGCTCCCG AGAGCCCCTG TGGACTGTGC TTGTGGGGAG CTGCCCCCTC CGTGCATTCA
    CCAACTTGTC CGTCCGTCCG CCCCCGGGGC ACCACTCCAT CCACCTCCTC ACATGGCTGG
    CTTCCTCGTC TGCCGCCGCC ACCACCGCTG CCTCCACTGC CTCTGGGGCC CCCCATTCTG
    TCTGAGTCCC CACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016877947.1
    CDS275..4012
    Translation

    Target ORF information:

    RefSeq Version XM_017022458.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022458.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TATATTGTGA TGATGTTGAC
    ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGCTA CGCCAGTGTG
    GAGCGGCTGC TGGAGAGGCT GACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC
    TTCCTGCACT CCTACCGCGT CTTCACCACC GCCATCGTGG TCCTGGACAA GCTCATTACC
    ATCTACAAGA AGCCTATCAG TGCCATTCCT GCCAGGTCGC TGGAGCTCCT GTTTGCCAGT
    GGCCAGAACA ATAAGCTCCT GTACGGTGAA CCCCCCAAGT CCCCGCGCGC CACCCGCAAG
    TTCTCCTCGC CGCCACCTCT GTCCATCACC AAGACATCGT CACCGAGCCG CCGGCGGAAG
    CTCTCCCTGA ACATCCCCAT CATCACTGGC GGCAAGGCCC TGGACCTGGC CGCCCTCAGC
    TGCAACTCCA ATGGCTACAC CAGCATGTAC TCGGCCATGT CACCCTTCAG CAAGGCCACG
    CTGGACACCA GCAAGCTCTA TGTGTCCAGC AGCTTCACCA ACAAGATTCC AGATGAGGGC
    GATACGACCC CTGAGAAGCC CGAAGACCCT TCAGCGCTCA GCAAGCAGAG CTCAGAAGTC
    TCCATGAGAG AGGAGTCAGA TATTGATCAA AACCAGAGTG ATGATGGTGA TACTGAAACA
    TCACCAACTA AATCTCCAAC AACACCCAAA TCAGTCAAAA ACAAAAATTC TTCAGAGTTC
    CCACTCTTTT CCTATAACAA TGGAGTCGTC ATGACCTCCT GTCGTGAACT GGACAATAAC
    CGCAGTGCCT TGTCGGCCGC CTCTGCCTTT GCCATAGCAA CCGCCGGGGC CAACGAGGGC
    ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTAGCCA GTGCAGGGTT TCCCCCAGAC
    CAGAGGAATG GAGACAAGGA GTTTGTGATC CGCAGAGCAG CCACCAATCG TGTCTTGAAC
    GTGCTCCGCC ACTGGGTGTC CAAGCACTCT CAGGACTTTG AGACCAACGA TGAGCTCAAA
    TGCAAGGTGA TCGGCTTCCT GGAAGAAGTC ATGCACGACC CGGAGCTCCT GACCCAGGAG
    CGGAAGGCTG CAGCCAACAT CATCAGGACT CTGACCCAGG AGGACCCAGG TGACAACCAG
    ATCACGCTGG AGGAGATCAC GCAGATGGCT GAAGGCGTGA AGGCTGAGCC CTTTGAAAAC
    CACTCAGCCC TGGAGATCGC GGAGCAGCTG ACCCTGCTAG ATCACCTCGT CTTCAAGAAG
    ATTCCTTATG AGGAGTTCTT CGGACAAGGA TGGATGAAAC TGGAAAAGAA TGAAAGGACC
    CCTTATATCA TGAAAACCAC TAAGCACTTC AATGACATCA GTAACTTGAT TGCTTCAGAA
    ATCATCCGCA ATGAGGACAT CAACGCCAGG GTGAGCGCCA TCGAGAAGTG GGTGGCCGTA
    GCTGACATAT GCCGCTGCCT CCACAACTAC AATGCCGTAC TGGAGATCAC CTCGTCCATG
    AACCGCAGTG CAATCTTCCG GCTCAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGACTAAA
    GCTTTGATTG ATAAGCTCCA AAAGCTTGTG TCATCTGAGG GCAGATTTAA GAATCTCAGA
    GAAGCTCTGA AAAAACAGAT GTTGCACCCA TCTGTGCTGT CCAGCCGTCT CTCTTCTGCC
    TGGGCTCCCG AGAGCCCCTG TGGACTGTGC TTGTGGGGAG CTGCCCCCTC CGTGCATTCA
    CCAACTTGTC CGTCCGTCCG CCCCCGGGGC ACCACTCCAT CCACCTCCTC ACATGGCTGG
    CTTCCTCGTC TGCCGCCGCC ACCACCGCTG CCTCCACTGC CTCTGGGGCC CCCCATTCTG
    TCTGAGTCCC CACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85585
    Clone ID Related Accession (Same CDS sequence) XM_017022458.2 , XM_017022458.1
    Accession Version XM_017022458.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3738bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ras-specific guanine nucleotide-releasing factor 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TATATTGTGA TGATGTTGAC
    ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGCTA CGCCAGTGTG
    GAGCGGCTGC TGGAGAGGCT GACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC
    TTCCTGCACT CCTACCGCGT CTTCACCACC GCCATCGTGG TCCTGGACAA GCTCATTACC
    ATCTACAAGA AGCCTATCAG TGCCATTCCT GCCAGGTCGC TGGAGCTCCT GTTTGCCAGT
    GGCCAGAACA ATAAGCTCCT GTACGGTGAA CCCCCCAAGT CCCCGCGCGC CACCCGCAAG
    TTCTCCTCGC CGCCACCTCT GTCCATCACC AAGACATCGT CACCGAGCCG CCGGCGGAAG
    CTCTCCCTGA ACATCCCCAT CATCACTGGC GGCAAGGCCC TGGACCTGGC CGCCCTCAGC
    TGCAACTCCA ATGGCTACAC CAGCATGTAC TCGGCCATGT CACCCTTCAG CAAGGCCACG
    CTGGACACCA GCAAGCTCTA TGTGTCCAGC AGCTTCACCA ACAAGATTCC AGATGAGGGC
    GATACGACCC CTGAGAAGCC CGAAGACCCT TCAGCGCTCA GCAAGCAGAG CTCAGAAGTC
    TCCATGAGAG AGGAGTCAGA TATTGATCAA AACCAGAGTG ATGATGGTGA TACTGAAACA
    TCACCAACTA AATCTCCAAC AACACCCAAA TCAGTCAAAA ACAAAAATTC TTCAGAGTTC
    CCACTCTTTT CCTATAACAA TGGAGTCGTC ATGACCTCCT GTCGTGAACT GGACAATAAC
    CGCAGTGCCT TGTCGGCCGC CTCTGCCTTT GCCATAGCAA CCGCCGGGGC CAACGAGGGC
    ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTAGCCA GTGCAGGGTT TCCCCCAGAC
    CAGAGGAATG GAGACAAGGA GTTTGTGATC CGCAGAGCAG CCACCAATCG TGTCTTGAAC
    GTGCTCCGCC ACTGGGTGTC CAAGCACTCT CAGGACTTTG AGACCAACGA TGAGCTCAAA
    TGCAAGGTGA TCGGCTTCCT GGAAGAAGTC ATGCACGACC CGGAGCTCCT GACCCAGGAG
    CGGAAGGCTG CAGCCAACAT CATCAGGACT CTGACCCAGG AGGACCCAGG TGACAACCAG
    ATCACGCTGG AGGAGATCAC GCAGATGGCT GAAGGCGTGA AGGCTGAGCC CTTTGAAAAC
    CACTCAGCCC TGGAGATCGC GGAGCAGCTG ACCCTGCTAG ATCACCTCGT CTTCAAGAAG
    ATTCCTTATG AGGAGTTCTT CGGACAAGGA TGGATGAAAC TGGAAAAGAA TGAAAGGACC
    CCTTATATCA TGAAAACCAC TAAGCACTTC AATGACATCA GTAACTTGAT TGCTTCAGAA
    ATCATCCGCA ATGAGGACAT CAACGCCAGG GTGAGCGCCA TCGAGAAGTG GGTGGCCGTA
    GCTGACATAT GCCGCTGCCT CCACAACTAC AATGCCGTAC TGGAGATCAC CTCGTCCATG
    AACCGCAGTG CAATCTTCCG GCTCAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGACTAAA
    GCTTTGATTG ATAAGCTCCA AAAGCTTGTG TCATCTGAGG GCAGATTTAA GAATCTCAGA
    GAAGCTCTGA AAAAACAGAT GTTGCACCCA TCTGTGCTGT CCAGCCGTCT CTCTTCTGCC
    TGGGCTCCCG AGAGCCCCTG TGGACTGTGC TTGTGGGGAG CTGCCCCCTC CGTGCATTCA
    CCAACTTGTC CGTCCGTCCG CCCCCGGGGC ACCACTCCAT CCACCTCCTC ACATGGCTGG
    CTTCCTCGTC TGCCGCCGCC ACCACCGCTG CCTCCACTGC CTCTGGGGCC CCCCATTCTG
    TCTGAGTCCC CACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016877947.1
    CDS276..4013
    Translation

    Target ORF information:

    RefSeq Version XM_017022458.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022458.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TATATTGTGA TGATGTTGAC
    ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGCTA CGCCAGTGTG
    GAGCGGCTGC TGGAGAGGCT GACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC
    TTCCTGCACT CCTACCGCGT CTTCACCACC GCCATCGTGG TCCTGGACAA GCTCATTACC
    ATCTACAAGA AGCCTATCAG TGCCATTCCT GCCAGGTCGC TGGAGCTCCT GTTTGCCAGT
    GGCCAGAACA ATAAGCTCCT GTACGGTGAA CCCCCCAAGT CCCCGCGCGC CACCCGCAAG
    TTCTCCTCGC CGCCACCTCT GTCCATCACC AAGACATCGT CACCGAGCCG CCGGCGGAAG
    CTCTCCCTGA ACATCCCCAT CATCACTGGC GGCAAGGCCC TGGACCTGGC CGCCCTCAGC
    TGCAACTCCA ATGGCTACAC CAGCATGTAC TCGGCCATGT CACCCTTCAG CAAGGCCACG
    CTGGACACCA GCAAGCTCTA TGTGTCCAGC AGCTTCACCA ACAAGATTCC AGATGAGGGC
    GATACGACCC CTGAGAAGCC CGAAGACCCT TCAGCGCTCA GCAAGCAGAG CTCAGAAGTC
    TCCATGAGAG AGGAGTCAGA TATTGATCAA AACCAGAGTG ATGATGGTGA TACTGAAACA
    TCACCAACTA AATCTCCAAC AACACCCAAA TCAGTCAAAA ACAAAAATTC TTCAGAGTTC
    CCACTCTTTT CCTATAACAA TGGAGTCGTC ATGACCTCCT GTCGTGAACT GGACAATAAC
    CGCAGTGCCT TGTCGGCCGC CTCTGCCTTT GCCATAGCAA CCGCCGGGGC CAACGAGGGC
    ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTAGCCA GTGCAGGGTT TCCCCCAGAC
    CAGAGGAATG GAGACAAGGA GTTTGTGATC CGCAGAGCAG CCACCAATCG TGTCTTGAAC
    GTGCTCCGCC ACTGGGTGTC CAAGCACTCT CAGGACTTTG AGACCAACGA TGAGCTCAAA
    TGCAAGGTGA TCGGCTTCCT GGAAGAAGTC ATGCACGACC CGGAGCTCCT GACCCAGGAG
    CGGAAGGCTG CAGCCAACAT CATCAGGACT CTGACCCAGG AGGACCCAGG TGACAACCAG
    ATCACGCTGG AGGAGATCAC GCAGATGGCT GAAGGCGTGA AGGCTGAGCC CTTTGAAAAC
    CACTCAGCCC TGGAGATCGC GGAGCAGCTG ACCCTGCTAG ATCACCTCGT CTTCAAGAAG
    ATTCCTTATG AGGAGTTCTT CGGACAAGGA TGGATGAAAC TGGAAAAGAA TGAAAGGACC
    CCTTATATCA TGAAAACCAC TAAGCACTTC AATGACATCA GTAACTTGAT TGCTTCAGAA
    ATCATCCGCA ATGAGGACAT CAACGCCAGG GTGAGCGCCA TCGAGAAGTG GGTGGCCGTA
    GCTGACATAT GCCGCTGCCT CCACAACTAC AATGCCGTAC TGGAGATCAC CTCGTCCATG
    AACCGCAGTG CAATCTTCCG GCTCAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGACTAAA
    GCTTTGATTG ATAAGCTCCA AAAGCTTGTG TCATCTGAGG GCAGATTTAA GAATCTCAGA
    GAAGCTCTGA AAAAACAGAT GTTGCACCCA TCTGTGCTGT CCAGCCGTCT CTCTTCTGCC
    TGGGCTCCCG AGAGCCCCTG TGGACTGTGC TTGTGGGGAG CTGCCCCCTC CGTGCATTCA
    CCAACTTGTC CGTCCGTCCG CCCCCGGGGC ACCACTCCAT CCACCTCCTC ACATGGCTGG
    CTTCCTCGTC TGCCGCCGCC ACCACCGCTG CCTCCACTGC CTCTGGGGCC CCCCATTCTG
    TCTGAGTCCC CACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85586
    Clone ID Related Accession (Same CDS sequence) XM_017022459.2 , XM_017022459.1
    Accession Version XM_017022459.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3558bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ras-specific guanine nucleotide-releasing factor 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017022459.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TATATTGTGA TGATGTTGAC
    ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGCTA CGCCAGTGTG
    GAGCGGCTGC TGGAGAGGCT GACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC
    TTCCTGCACT CCTACCGCGT CTTCACCACC GCCATCGTGG TCCTGGACAA GCTCATTACC
    ATCTACAAGA AGCCTATCAG TGCCATTCCT GCCAGGTGGC TGAGGTCGCT GGAGCTCCTG
    TTTGCCAGTG GCCAGAACAA TAAGCTCCTG TACGGTGAAC CCCCCAAGTC CCCGCGCGCC
    ACCCGCAAGT TCTCCTCGCC GCCACCTCTG TCCATCACCA AGACATCGTC ACCGAGCCGC
    CGGCGGAAGC TCTCCCTGAA CATCCCCATC ATCACTGGCG GCAAGGCCCT GGACCTGGCC
    GCCCTCAGCT GCAACTCCAA TGGCTACACC AGCATGTACT CGGCCATGTC ACCCTTCAGC
    AAGGCCACGC TGGACACCAG CAAGCTCTAT GTGTCCAGCA GCTTCACCAA CAAGATTCCA
    GATGAGGGCG ATACGACCCC TGAGAAGCCC GAAGACCCTT CAGCGCTCAG CAAGCAGAGC
    TCAGAAGTCT CCATGAGAGA GGAGTCAGAT ATTGATCAAA ACCAGAGTGA TGATGGTGAT
    ACTGAAACAT CACCAACTAA ATCTCCAACA ACACCCAAAT CAGTCAAAAA CAAAAATTCT
    TCAGAGTTCC CACTCTTTTC CTATAACAAT GGAGTCGTCA TGACCTCCTG TCGTGAACTG
    GACAATAACC GCAGTGCCTT GTCGGCCGCC TCTGCCTTTG CCATAGCAAC CGCCGGGGCC
    AACGAGGGCA CCCCAAACAA GGAGAAGTAC CGGAGGATGT CCTTAGCCAG TGCAGGGTTT
    CCCCCAGACC AGAGGAATGG AGACAAGGAG TTTGTGATCC GCAGAGCAGC CACCAATCGT
    GTCTTGAACG TGCTCCGCCA CTGGGTGTCC AAGCACTCTC AGGACTTTGA GACCAACGAT
    GAGCTCAAAT GCAAGGTGAT CGGCTTCCTG GAAGAAGTCA TGCACGACCC GGAGCTCCTG
    ACCCAGGAGC GGAAGGCTGC AGCCAACATC ATCAGGACTC TGACCCAGGA GGACCCAGGT
    GACAACCAGA TCACGCTGGA GGAGATCACG CAGATGGCTG AAGGCGTGAA GGCTGAGCCC
    TTTGAAAACC ACTCAGCCCT GGAGATCGCG GAGCAGCTGA CCCTGCTAGA TCACCTCGTC
    TTCAAGAAGA TTCCTTATGA GGAGTTCTTC GGACAAGGAT GGATGAAACT GGAAAAGAAT
    GAAAGGACCC CTTATATCAT GAAAACCACT AAGCACTTCA ATGACATCAG TAACTTGATT
    GCTTCAGAAA TCATCCGCAA TGAGGACATC AACGCCAGGG TGAGCGCCAT CGAGAAGTGG
    GTGGCCGTAG CTGACATATG CCGCTGCCTC CACAACTACA ATGCCGTACT GGAGATCACC
    TCGTCCATGA ACCGCAGTGC AATCTTCCGG CTCAAAAAGA CGTGGCTCAA AGTCTCTAAG
    CAGACTAAAG CTTTGATTGA TAAGCTCCAA AAGCTTGTGT CATCTGAGGG CAGATTTAAG
    AATCTCAGAG AAGCTCTGAA AAAATATCCC ATATTATCCG AGAGATTCGC CAGTTTCAAC
    AAACTGCCTA CAAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016877948.1
    CDS275..3832
    Translation

    Target ORF information:

    RefSeq Version XM_017022459.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022459.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TATATTGTGA TGATGTTGAC
    ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGCTA CGCCAGTGTG
    GAGCGGCTGC TGGAGAGGCT GACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC
    TTCCTGCACT CCTACCGCGT CTTCACCACC GCCATCGTGG TCCTGGACAA GCTCATTACC
    ATCTACAAGA AGCCTATCAG TGCCATTCCT GCCAGGTGGC TGAGGTCGCT GGAGCTCCTG
    TTTGCCAGTG GCCAGAACAA TAAGCTCCTG TACGGTGAAC CCCCCAAGTC CCCGCGCGCC
    ACCCGCAAGT TCTCCTCGCC GCCACCTCTG TCCATCACCA AGACATCGTC ACCGAGCCGC
    CGGCGGAAGC TCTCCCTGAA CATCCCCATC ATCACTGGCG GCAAGGCCCT GGACCTGGCC
    GCCCTCAGCT GCAACTCCAA TGGCTACACC AGCATGTACT CGGCCATGTC ACCCTTCAGC
    AAGGCCACGC TGGACACCAG CAAGCTCTAT GTGTCCAGCA GCTTCACCAA CAAGATTCCA
    GATGAGGGCG ATACGACCCC TGAGAAGCCC GAAGACCCTT CAGCGCTCAG CAAGCAGAGC
    TCAGAAGTCT CCATGAGAGA GGAGTCAGAT ATTGATCAAA ACCAGAGTGA TGATGGTGAT
    ACTGAAACAT CACCAACTAA ATCTCCAACA ACACCCAAAT CAGTCAAAAA CAAAAATTCT
    TCAGAGTTCC CACTCTTTTC CTATAACAAT GGAGTCGTCA TGACCTCCTG TCGTGAACTG
    GACAATAACC GCAGTGCCTT GTCGGCCGCC TCTGCCTTTG CCATAGCAAC CGCCGGGGCC
    AACGAGGGCA CCCCAAACAA GGAGAAGTAC CGGAGGATGT CCTTAGCCAG TGCAGGGTTT
    CCCCCAGACC AGAGGAATGG AGACAAGGAG TTTGTGATCC GCAGAGCAGC CACCAATCGT
    GTCTTGAACG TGCTCCGCCA CTGGGTGTCC AAGCACTCTC AGGACTTTGA GACCAACGAT
    GAGCTCAAAT GCAAGGTGAT CGGCTTCCTG GAAGAAGTCA TGCACGACCC GGAGCTCCTG
    ACCCAGGAGC GGAAGGCTGC AGCCAACATC ATCAGGACTC TGACCCAGGA GGACCCAGGT
    GACAACCAGA TCACGCTGGA GGAGATCACG CAGATGGCTG AAGGCGTGAA GGCTGAGCCC
    TTTGAAAACC ACTCAGCCCT GGAGATCGCG GAGCAGCTGA CCCTGCTAGA TCACCTCGTC
    TTCAAGAAGA TTCCTTATGA GGAGTTCTTC GGACAAGGAT GGATGAAACT GGAAAAGAAT
    GAAAGGACCC CTTATATCAT GAAAACCACT AAGCACTTCA ATGACATCAG TAACTTGATT
    GCTTCAGAAA TCATCCGCAA TGAGGACATC AACGCCAGGG TGAGCGCCAT CGAGAAGTGG
    GTGGCCGTAG CTGACATATG CCGCTGCCTC CACAACTACA ATGCCGTACT GGAGATCACC
    TCGTCCATGA ACCGCAGTGC AATCTTCCGG CTCAAAAAGA CGTGGCTCAA AGTCTCTAAG
    CAGACTAAAG CTTTGATTGA TAAGCTCCAA AAGCTTGTGT CATCTGAGGG CAGATTTAAG
    AATCTCAGAG AAGCTCTGAA AAAATATCCC ATATTATCCG AGAGATTCGC CAGTTTCAAC
    AAACTGCCTA CAAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85586
    Clone ID Related Accession (Same CDS sequence) XM_017022459.2 , XM_017022459.1
    Accession Version XM_017022459.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3558bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ras-specific guanine nucleotide-releasing factor 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TATATTGTGA TGATGTTGAC
    ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGCTA CGCCAGTGTG
    GAGCGGCTGC TGGAGAGGCT GACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC
    TTCCTGCACT CCTACCGCGT CTTCACCACC GCCATCGTGG TCCTGGACAA GCTCATTACC
    ATCTACAAGA AGCCTATCAG TGCCATTCCT GCCAGGTGGC TGAGGTCGCT GGAGCTCCTG
    TTTGCCAGTG GCCAGAACAA TAAGCTCCTG TACGGTGAAC CCCCCAAGTC CCCGCGCGCC
    ACCCGCAAGT TCTCCTCGCC GCCACCTCTG TCCATCACCA AGACATCGTC ACCGAGCCGC
    CGGCGGAAGC TCTCCCTGAA CATCCCCATC ATCACTGGCG GCAAGGCCCT GGACCTGGCC
    GCCCTCAGCT GCAACTCCAA TGGCTACACC AGCATGTACT CGGCCATGTC ACCCTTCAGC
    AAGGCCACGC TGGACACCAG CAAGCTCTAT GTGTCCAGCA GCTTCACCAA CAAGATTCCA
    GATGAGGGCG ATACGACCCC TGAGAAGCCC GAAGACCCTT CAGCGCTCAG CAAGCAGAGC
    TCAGAAGTCT CCATGAGAGA GGAGTCAGAT ATTGATCAAA ACCAGAGTGA TGATGGTGAT
    ACTGAAACAT CACCAACTAA ATCTCCAACA ACACCCAAAT CAGTCAAAAA CAAAAATTCT
    TCAGAGTTCC CACTCTTTTC CTATAACAAT GGAGTCGTCA TGACCTCCTG TCGTGAACTG
    GACAATAACC GCAGTGCCTT GTCGGCCGCC TCTGCCTTTG CCATAGCAAC CGCCGGGGCC
    AACGAGGGCA CCCCAAACAA GGAGAAGTAC CGGAGGATGT CCTTAGCCAG TGCAGGGTTT
    CCCCCAGACC AGAGGAATGG AGACAAGGAG TTTGTGATCC GCAGAGCAGC CACCAATCGT
    GTCTTGAACG TGCTCCGCCA CTGGGTGTCC AAGCACTCTC AGGACTTTGA GACCAACGAT
    GAGCTCAAAT GCAAGGTGAT CGGCTTCCTG GAAGAAGTCA TGCACGACCC GGAGCTCCTG
    ACCCAGGAGC GGAAGGCTGC AGCCAACATC ATCAGGACTC TGACCCAGGA GGACCCAGGT
    GACAACCAGA TCACGCTGGA GGAGATCACG CAGATGGCTG AAGGCGTGAA GGCTGAGCCC
    TTTGAAAACC ACTCAGCCCT GGAGATCGCG GAGCAGCTGA CCCTGCTAGA TCACCTCGTC
    TTCAAGAAGA TTCCTTATGA GGAGTTCTTC GGACAAGGAT GGATGAAACT GGAAAAGAAT
    GAAAGGACCC CTTATATCAT GAAAACCACT AAGCACTTCA ATGACATCAG TAACTTGATT
    GCTTCAGAAA TCATCCGCAA TGAGGACATC AACGCCAGGG TGAGCGCCAT CGAGAAGTGG
    GTGGCCGTAG CTGACATATG CCGCTGCCTC CACAACTACA ATGCCGTACT GGAGATCACC
    TCGTCCATGA ACCGCAGTGC AATCTTCCGG CTCAAAAAGA CGTGGCTCAA AGTCTCTAAG
    CAGACTAAAG CTTTGATTGA TAAGCTCCAA AAGCTTGTGT CATCTGAGGG CAGATTTAAG
    AATCTCAGAG AAGCTCTGAA AAAATATCCC ATATTATCCG AGAGATTCGC CAGTTTCAAC
    AAACTGCCTA CAAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016877948.1
    CDS276..3833
    Translation

    Target ORF information:

    RefSeq Version XM_017022459.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022459.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TATATTGTGA TGATGTTGAC
    ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGCTA CGCCAGTGTG
    GAGCGGCTGC TGGAGAGGCT GACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC
    TTCCTGCACT CCTACCGCGT CTTCACCACC GCCATCGTGG TCCTGGACAA GCTCATTACC
    ATCTACAAGA AGCCTATCAG TGCCATTCCT GCCAGGTGGC TGAGGTCGCT GGAGCTCCTG
    TTTGCCAGTG GCCAGAACAA TAAGCTCCTG TACGGTGAAC CCCCCAAGTC CCCGCGCGCC
    ACCCGCAAGT TCTCCTCGCC GCCACCTCTG TCCATCACCA AGACATCGTC ACCGAGCCGC
    CGGCGGAAGC TCTCCCTGAA CATCCCCATC ATCACTGGCG GCAAGGCCCT GGACCTGGCC
    GCCCTCAGCT GCAACTCCAA TGGCTACACC AGCATGTACT CGGCCATGTC ACCCTTCAGC
    AAGGCCACGC TGGACACCAG CAAGCTCTAT GTGTCCAGCA GCTTCACCAA CAAGATTCCA
    GATGAGGGCG ATACGACCCC TGAGAAGCCC GAAGACCCTT CAGCGCTCAG CAAGCAGAGC
    TCAGAAGTCT CCATGAGAGA GGAGTCAGAT ATTGATCAAA ACCAGAGTGA TGATGGTGAT
    ACTGAAACAT CACCAACTAA ATCTCCAACA ACACCCAAAT CAGTCAAAAA CAAAAATTCT
    TCAGAGTTCC CACTCTTTTC CTATAACAAT GGAGTCGTCA TGACCTCCTG TCGTGAACTG
    GACAATAACC GCAGTGCCTT GTCGGCCGCC TCTGCCTTTG CCATAGCAAC CGCCGGGGCC
    AACGAGGGCA CCCCAAACAA GGAGAAGTAC CGGAGGATGT CCTTAGCCAG TGCAGGGTTT
    CCCCCAGACC AGAGGAATGG AGACAAGGAG TTTGTGATCC GCAGAGCAGC CACCAATCGT
    GTCTTGAACG TGCTCCGCCA CTGGGTGTCC AAGCACTCTC AGGACTTTGA GACCAACGAT
    GAGCTCAAAT GCAAGGTGAT CGGCTTCCTG GAAGAAGTCA TGCACGACCC GGAGCTCCTG
    ACCCAGGAGC GGAAGGCTGC AGCCAACATC ATCAGGACTC TGACCCAGGA GGACCCAGGT
    GACAACCAGA TCACGCTGGA GGAGATCACG CAGATGGCTG AAGGCGTGAA GGCTGAGCCC
    TTTGAAAACC ACTCAGCCCT GGAGATCGCG GAGCAGCTGA CCCTGCTAGA TCACCTCGTC
    TTCAAGAAGA TTCCTTATGA GGAGTTCTTC GGACAAGGAT GGATGAAACT GGAAAAGAAT
    GAAAGGACCC CTTATATCAT GAAAACCACT AAGCACTTCA ATGACATCAG TAACTTGATT
    GCTTCAGAAA TCATCCGCAA TGAGGACATC AACGCCAGGG TGAGCGCCAT CGAGAAGTGG
    GTGGCCGTAG CTGACATATG CCGCTGCCTC CACAACTACA ATGCCGTACT GGAGATCACC
    TCGTCCATGA ACCGCAGTGC AATCTTCCGG CTCAAAAAGA CGTGGCTCAA AGTCTCTAAG
    CAGACTAAAG CTTTGATTGA TAAGCTCCAA AAGCTTGTGT CATCTGAGGG CAGATTTAAG
    AATCTCAGAG AAGCTCTGAA AAAATATCCC ATATTATCCG AGAGATTCGC CAGTTTCAAC
    AAACTGCCTA CAAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu08312
    Clone ID Related Accession (Same CDS sequence) NM_001145648.1 , NM_001145648.2
    Accession Version NM_001145648.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3774bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-20
    Organism Homo sapiens(human)
    Product ras-specific guanine nucleotide-releasing factor 1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA126882.1, BC040275.1, AK226101.1, AC011944.12 and AL355696.1. Transcript Variant: This variant (3) lacks an in-frame coding exon, as compared to variant 1. The resulting isoform (3) lacks an internal segment, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.118444.1, SRR1660807.126484.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TATATTGTGA TGATGTTGAC
    ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGCTA CGCCAGTGTG
    GAGCGGCTGC TGGAGAGGCT GACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC
    TTCCTGCACT CCTACCGCGT CTTCACCACC GCCATCGTGG TCCTGGACAA GCTCATTACC
    ATCTACAAGA AGCCTATCAG TGCCATTCCT GCCAGGTCGC TGGAGCTCCT GTTTGCCAGT
    GGCCAGAACA ATAAGCTCCT GTACGGTGAA CCCCCCAAGT CCCCGCGCGC CACCCGCAAG
    TTCTCCTCGC CGCCACCTCT GTCCATCACC AAGACATCGT CACCGAGCCG CCGGCGGAAG
    CTCTCCCTGA ACATCCCCAT CATCACTGGC GGCAAGGCCC TGGACCTGGC CGCCCTCAGC
    TGCAACTCCA ATGGCTACAC CAGCATGTAC TCGGCCATGT CACCCTTCAG CAAGGCCACG
    CTGGACACCA GCAAGCTCTA TGTGTCCAGC AGCTTCACCA ACAAGATTCC AGATGAGGGC
    GATACGACCC CTGAGAAGCC CGAAGACCCT TCAGCGCTCA GCAAGCAGAG CTCAGAAGTC
    TCCATGAGAG AGGAGTCAGA TATTGATCAA AACCAGAGTG ATGATGGTGA TACTGAAACA
    TCACCAACTA AATCTCCAAC AACACCCAAA TCAGTCAAAA ACAAAAATTC TTCAGAGTTC
    CCACTCTTTT CCTATAACAA TGGAGTCGTC ATGACCTCCT GTCGTGAACT GGACAATAAC
    CGCAGTGCCT TGTCGGCCGC CTCTGCCTTT GCCATAGCAA CCGCCGGGGC CAACGAGGGC
    ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTAGCCA GTGCAGGGTT TCCCCCAGAC
    CAGAGGAATG GAGACAAGGA GTTTGTGATC CGCAGAGCAG CCACCAATCG TGTCTTGAAC
    GTGCTCCGCC ACTGGGTGTC CAAGCACTCT CAGGACTTTG AGACCAACGA TGAGCTCAAA
    TGCAAGGTGA TCGGCTTCCT GGAAGAAGTC ATGCACGACC CGGAGCTCCT GACCCAGGAG
    CGGAAGGCTG CAGCCAACAT CATCAGGACT CTGACCCAGG AGGACCCAGG TGACAACCAG
    ATCACGCTGG AGGAGATCAC GCAGATGGCT GAAGGCGTGA AGGCTGAGCC CTTTGAAAAC
    CACTCAGCCC TGGAGATCGC GGAGCAGCTG ACCCTGCTAG ATCACCTCGT CTTCAAGAAG
    ATTCCTTATG AGGAGTTCTT CGGACAAGGA TGGATGAAAC TGGAAAAGAA TGAAAGGACC
    CCTTATATCA TGAAAACCAC TAAGCACTTC AATGACATCA GTAACTTGAT TGCTTCAGAA
    ATCATCCGCA ATGAGGACAT CAACGCCAGG GTGAGCGCCA TCGAGAAGTG GGTGGCCGTA
    GCTGACATAT GCCGCTGCCT CCACAACTAC AATGCCGTAC TGGAGATCAC CTCGTCCATG
    AACCGCAGTG CAATCTTCCG GCTCAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGACTAAA
    GCTTTGATTG ATAAGCTCCA AAAGCTTGTG TCATCTGAGG GCAGATTTAA GAATCTCAGA
    GAAGCTCTGA AAAATTGTGA CCCACCCTGT GTCCCTTACC TGGGGATGTA CCTCACCGAC
    CTGGCCTTCA TCGAGGAGGG GACGCCCAAT TACACGGAAG ACGGCCTGGT CAACTTCTCC
    AAGATGAGGA TGATATCCCA TATTATCCGA GAGATTCGCC AGTTTCAACA AACTGCCTAC
    AAAATAGAGC ACCAAGCAAA GGTAACGCAA TATTTACTGG ACCAATCTTT TGTAATGGAT
    GAAGAAAGCC TCTACGAGTC TTCTCTCCGA ATAGAACCAA AACTCCCCAC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001139120.1
    CDS376..4149
    Translation

    Target ORF information:

    RefSeq Version NM_001145648.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001145648.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TATATTGTGA TGATGTTGAC
    ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGCTA CGCCAGTGTG
    GAGCGGCTGC TGGAGAGGCT GACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC
    TTCCTGCACT CCTACCGCGT CTTCACCACC GCCATCGTGG TCCTGGACAA GCTCATTACC
    ATCTACAAGA AGCCTATCAG TGCCATTCCT GCCAGGTCGC TGGAGCTCCT GTTTGCCAGT
    GGCCAGAACA ATAAGCTCCT GTACGGTGAA CCCCCCAAGT CCCCGCGCGC CACCCGCAAG
    TTCTCCTCGC CGCCACCTCT GTCCATCACC AAGACATCGT CACCGAGCCG CCGGCGGAAG
    CTCTCCCTGA ACATCCCCAT CATCACTGGC GGCAAGGCCC TGGACCTGGC CGCCCTCAGC
    TGCAACTCCA ATGGCTACAC CAGCATGTAC TCGGCCATGT CACCCTTCAG CAAGGCCACG
    CTGGACACCA GCAAGCTCTA TGTGTCCAGC AGCTTCACCA ACAAGATTCC AGATGAGGGC
    GATACGACCC CTGAGAAGCC CGAAGACCCT TCAGCGCTCA GCAAGCAGAG CTCAGAAGTC
    TCCATGAGAG AGGAGTCAGA TATTGATCAA AACCAGAGTG ATGATGGTGA TACTGAAACA
    TCACCAACTA AATCTCCAAC AACACCCAAA TCAGTCAAAA ACAAAAATTC TTCAGAGTTC
    CCACTCTTTT CCTATAACAA TGGAGTCGTC ATGACCTCCT GTCGTGAACT GGACAATAAC
    CGCAGTGCCT TGTCGGCCGC CTCTGCCTTT GCCATAGCAA CCGCCGGGGC CAACGAGGGC
    ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTAGCCA GTGCAGGGTT TCCCCCAGAC
    CAGAGGAATG GAGACAAGGA GTTTGTGATC CGCAGAGCAG CCACCAATCG TGTCTTGAAC
    GTGCTCCGCC ACTGGGTGTC CAAGCACTCT CAGGACTTTG AGACCAACGA TGAGCTCAAA
    TGCAAGGTGA TCGGCTTCCT GGAAGAAGTC ATGCACGACC CGGAGCTCCT GACCCAGGAG
    CGGAAGGCTG CAGCCAACAT CATCAGGACT CTGACCCAGG AGGACCCAGG TGACAACCAG
    ATCACGCTGG AGGAGATCAC GCAGATGGCT GAAGGCGTGA AGGCTGAGCC CTTTGAAAAC
    CACTCAGCCC TGGAGATCGC GGAGCAGCTG ACCCTGCTAG ATCACCTCGT CTTCAAGAAG
    ATTCCTTATG AGGAGTTCTT CGGACAAGGA TGGATGAAAC TGGAAAAGAA TGAAAGGACC
    CCTTATATCA TGAAAACCAC TAAGCACTTC AATGACATCA GTAACTTGAT TGCTTCAGAA
    ATCATCCGCA ATGAGGACAT CAACGCCAGG GTGAGCGCCA TCGAGAAGTG GGTGGCCGTA
    GCTGACATAT GCCGCTGCCT CCACAACTAC AATGCCGTAC TGGAGATCAC CTCGTCCATG
    AACCGCAGTG CAATCTTCCG GCTCAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGACTAAA
    GCTTTGATTG ATAAGCTCCA AAAGCTTGTG TCATCTGAGG GCAGATTTAA GAATCTCAGA
    GAAGCTCTGA AAAATTGTGA CCCACCCTGT GTCCCTTACC TGGGGATGTA CCTCACCGAC
    CTGGCCTTCA TCGAGGAGGG GACGCCCAAT TACACGGAAG ACGGCCTGGT CAACTTCTCC
    AAGATGAGGA TGATATCCCA TATTATCCGA GAGATTCGCC AGTTTCAACA AACTGCCTAC
    AAAATAGAGC ACCAAGCAAA GGTAACGCAA TATTTACTGG ACCAATCTTT TGTAATGGAT
    GAAGAAAGCC TCTACGAGTC TTCTCTCCGA ATAGAACCAA AACTCCCCAC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu08312
    Clone ID Related Accession (Same CDS sequence) NM_001145648.1 , NM_001145648.2
    Accession Version NM_001145648.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3774bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Homo sapiens(human)
    Product ras-specific guanine nucleotide-releasing factor 1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA126882.1, BC040275.1, AK226101.1, AC011944.12 and AL355696.1. On Jun 2, 2019 this sequence version replaced NM_001145648.1. Transcript Variant: This variant (3) lacks an in-frame coding exon, as compared to variant 1. The resulting isoform (3) lacks an internal segment, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.126484.1, SRR1803614.118444.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TATATTGTGA TGATGTTGAC
    ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGCTA CGCCAGTGTG
    GAGCGGCTGC TGGAGAGGCT GACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC
    TTCCTGCACT CCTACCGCGT CTTCACCACC GCCATCGTGG TCCTGGACAA GCTCATTACC
    ATCTACAAGA AGCCTATCAG TGCCATTCCT GCCAGGTCGC TGGAGCTCCT GTTTGCCAGT
    GGCCAGAACA ATAAGCTCCT GTACGGTGAA CCCCCCAAGT CCCCGCGCGC CACCCGCAAG
    TTCTCCTCGC CGCCACCTCT GTCCATCACC AAGACATCGT CACCGAGCCG CCGGCGGAAG
    CTCTCCCTGA ACATCCCCAT CATCACTGGC GGCAAGGCCC TGGACCTGGC CGCCCTCAGC
    TGCAACTCCA ATGGCTACAC CAGCATGTAC TCGGCCATGT CACCCTTCAG CAAGGCCACG
    CTGGACACCA GCAAGCTCTA TGTGTCCAGC AGCTTCACCA ACAAGATTCC AGATGAGGGC
    GATACGACCC CTGAGAAGCC CGAAGACCCT TCAGCGCTCA GCAAGCAGAG CTCAGAAGTC
    TCCATGAGAG AGGAGTCAGA TATTGATCAA AACCAGAGTG ATGATGGTGA TACTGAAACA
    TCACCAACTA AATCTCCAAC AACACCCAAA TCAGTCAAAA ACAAAAATTC TTCAGAGTTC
    CCACTCTTTT CCTATAACAA TGGAGTCGTC ATGACCTCCT GTCGTGAACT GGACAATAAC
    CGCAGTGCCT TGTCGGCCGC CTCTGCCTTT GCCATAGCAA CCGCCGGGGC CAACGAGGGC
    ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTAGCCA GTGCAGGGTT TCCCCCAGAC
    CAGAGGAATG GAGACAAGGA GTTTGTGATC CGCAGAGCAG CCACCAATCG TGTCTTGAAC
    GTGCTCCGCC ACTGGGTGTC CAAGCACTCT CAGGACTTTG AGACCAACGA TGAGCTCAAA
    TGCAAGGTGA TCGGCTTCCT GGAAGAAGTC ATGCACGACC CGGAGCTCCT GACCCAGGAG
    CGGAAGGCTG CAGCCAACAT CATCAGGACT CTGACCCAGG AGGACCCAGG TGACAACCAG
    ATCACGCTGG AGGAGATCAC GCAGATGGCT GAAGGCGTGA AGGCTGAGCC CTTTGAAAAC
    CACTCAGCCC TGGAGATCGC GGAGCAGCTG ACCCTGCTAG ATCACCTCGT CTTCAAGAAG
    ATTCCTTATG AGGAGTTCTT CGGACAAGGA TGGATGAAAC TGGAAAAGAA TGAAAGGACC
    CCTTATATCA TGAAAACCAC TAAGCACTTC AATGACATCA GTAACTTGAT TGCTTCAGAA
    ATCATCCGCA ATGAGGACAT CAACGCCAGG GTGAGCGCCA TCGAGAAGTG GGTGGCCGTA
    GCTGACATAT GCCGCTGCCT CCACAACTAC AATGCCGTAC TGGAGATCAC CTCGTCCATG
    AACCGCAGTG CAATCTTCCG GCTCAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGACTAAA
    GCTTTGATTG ATAAGCTCCA AAAGCTTGTG TCATCTGAGG GCAGATTTAA GAATCTCAGA
    GAAGCTCTGA AAAATTGTGA CCCACCCTGT GTCCCTTACC TGGGGATGTA CCTCACCGAC
    CTGGCCTTCA TCGAGGAGGG GACGCCCAAT TACACGGAAG ACGGCCTGGT CAACTTCTCC
    AAGATGAGGA TGATATCCCA TATTATCCGA GAGATTCGCC AGTTTCAACA AACTGCCTAC
    AAAATAGAGC ACCAAGCAAA GGTAACGCAA TATTTACTGG ACCAATCTTT TGTAATGGAT
    GAAGAAAGCC TCTACGAGTC TTCTCTCCGA ATAGAACCAA AACTCCCCAC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001139120.1
    CDS283..4056
    Translation

    Target ORF information:

    RefSeq Version NM_001145648.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001145648.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TATATTGTGA TGATGTTGAC
    ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGCTA CGCCAGTGTG
    GAGCGGCTGC TGGAGAGGCT GACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC
    TTCCTGCACT CCTACCGCGT CTTCACCACC GCCATCGTGG TCCTGGACAA GCTCATTACC
    ATCTACAAGA AGCCTATCAG TGCCATTCCT GCCAGGTCGC TGGAGCTCCT GTTTGCCAGT
    GGCCAGAACA ATAAGCTCCT GTACGGTGAA CCCCCCAAGT CCCCGCGCGC CACCCGCAAG
    TTCTCCTCGC CGCCACCTCT GTCCATCACC AAGACATCGT CACCGAGCCG CCGGCGGAAG
    CTCTCCCTGA ACATCCCCAT CATCACTGGC GGCAAGGCCC TGGACCTGGC CGCCCTCAGC
    TGCAACTCCA ATGGCTACAC CAGCATGTAC TCGGCCATGT CACCCTTCAG CAAGGCCACG
    CTGGACACCA GCAAGCTCTA TGTGTCCAGC AGCTTCACCA ACAAGATTCC AGATGAGGGC
    GATACGACCC CTGAGAAGCC CGAAGACCCT TCAGCGCTCA GCAAGCAGAG CTCAGAAGTC
    TCCATGAGAG AGGAGTCAGA TATTGATCAA AACCAGAGTG ATGATGGTGA TACTGAAACA
    TCACCAACTA AATCTCCAAC AACACCCAAA TCAGTCAAAA ACAAAAATTC TTCAGAGTTC
    CCACTCTTTT CCTATAACAA TGGAGTCGTC ATGACCTCCT GTCGTGAACT GGACAATAAC
    CGCAGTGCCT TGTCGGCCGC CTCTGCCTTT GCCATAGCAA CCGCCGGGGC CAACGAGGGC
    ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTAGCCA GTGCAGGGTT TCCCCCAGAC
    CAGAGGAATG GAGACAAGGA GTTTGTGATC CGCAGAGCAG CCACCAATCG TGTCTTGAAC
    GTGCTCCGCC ACTGGGTGTC CAAGCACTCT CAGGACTTTG AGACCAACGA TGAGCTCAAA
    TGCAAGGTGA TCGGCTTCCT GGAAGAAGTC ATGCACGACC CGGAGCTCCT GACCCAGGAG
    CGGAAGGCTG CAGCCAACAT CATCAGGACT CTGACCCAGG AGGACCCAGG TGACAACCAG
    ATCACGCTGG AGGAGATCAC GCAGATGGCT GAAGGCGTGA AGGCTGAGCC CTTTGAAAAC
    CACTCAGCCC TGGAGATCGC GGAGCAGCTG ACCCTGCTAG ATCACCTCGT CTTCAAGAAG
    ATTCCTTATG AGGAGTTCTT CGGACAAGGA TGGATGAAAC TGGAAAAGAA TGAAAGGACC
    CCTTATATCA TGAAAACCAC TAAGCACTTC AATGACATCA GTAACTTGAT TGCTTCAGAA
    ATCATCCGCA ATGAGGACAT CAACGCCAGG GTGAGCGCCA TCGAGAAGTG GGTGGCCGTA
    GCTGACATAT GCCGCTGCCT CCACAACTAC AATGCCGTAC TGGAGATCAC CTCGTCCATG
    AACCGCAGTG CAATCTTCCG GCTCAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGACTAAA
    GCTTTGATTG ATAAGCTCCA AAAGCTTGTG TCATCTGAGG GCAGATTTAA GAATCTCAGA
    GAAGCTCTGA AAAATTGTGA CCCACCCTGT GTCCCTTACC TGGGGATGTA CCTCACCGAC
    CTGGCCTTCA TCGAGGAGGG GACGCCCAAT TACACGGAAG ACGGCCTGGT CAACTTCTCC
    AAGATGAGGA TGATATCCCA TATTATCCGA GAGATTCGCC AGTTTCAACA AACTGCCTAC
    AAAATAGAGC ACCAAGCAAA GGTAACGCAA TATTTACTGG ACCAATCTTT TGTAATGGAT
    GAAGAAAGCC TCTACGAGTC TTCTCTCCGA ATAGAACCAA AACTCCCCAC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu08508
    Clone ID Related Accession (Same CDS sequence) NM_002891.5 , NM_002891.4
    Accession Version NM_002891.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3822bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Homo sapiens(human)
    Product ras-specific guanine nucleotide-releasing factor 1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA126882.1, AK290135.1, AK226101.1, AC011944.12 and AL355696.1. On Jun 2, 2019 this sequence version replaced NM_002891.4. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.187641.1, L26584.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGAGG ACCAGGGAGG GGACCAGGGA AGCAGAAATG
    AGCAGGTCCG ACGCCTCCTT ATATTGTGAT GATGTTGACA TTCGCTTCAG CAAAACCATG
    AACTCCTGCA AAGTGCTGCA GATCCGCTAC GCCAGTGTGG AGCGGCTGCT GGAGAGGCTG
    ACGGACCTGC GCTTCCTGAG CATCGACTTC CTCAACACCT TCCTGCACTC CTACCGCGTC
    TTCACCACCG CCATCGTGGT CCTGGACAAG CTCATTACCA TCTACAAGAA GCCTATCAGT
    GCCATTCCTG CCAGGTGGCT GAGGTCGCTG GAGCTCCTGT TTGCCAGTGG CCAGAACAAT
    AAGCTCCTGT ACGGTGAACC CCCCAAGTCC CCGCGCGCCA CCCGCAAGTT CTCCTCGCCG
    CCACCTCTGT CCATCACCAA GACATCGTCA CCGAGCCGCC GGCGGAAGCT CTCCCTGAAC
    ATCCCCATCA TCACTGGCGG CAAGGCCCTG GACCTGGCCG CCCTCAGCTG CAACTCCAAT
    GGCTACACCA GCATGTACTC GGCCATGTCA CCCTTCAGCA AGGCCACGCT GGACACCAGC
    AAGCTCTATG TGTCCAGCAG CTTCACCAAC AAGATTCCAG ATGAGGGCGA TACGACCCCT
    GAGAAGCCCG AAGACCCTTC AGCGCTCAGC AAGCAGAGCT CAGAAGTCTC CATGAGAGAG
    GAGTCAGATA TTGATCAAAA CCAGAGTGAT GATGGTGATA CTGAAACATC ACCAACTAAA
    TCTCCAACAA CACCCAAATC AGTCAAAAAC AAAAATTCTT CAGAGTTCCC ACTCTTTTCC
    TATAACAATG GAGTCGTCAT GACCTCCTGT CGTGAACTGG ACAATAACCG CAGTGCCTTG
    TCGGCCGCCT CTGCCTTTGC CATAGCAACC GCCGGGGCCA ACGAGGGCAC CCCAAACAAG
    GAGAAGTACC GGAGGATGTC CTTAGCCAGT GCAGGGTTTC CCCCAGACCA GAGGAATGGA
    GACAAGGAGT TTGTGATCCG CAGAGCAGCC ACCAATCGTG TCTTGAACGT GCTCCGCCAC
    TGGGTGTCCA AGCACTCTCA GGACTTTGAG ACCAACGATG AGCTCAAATG CAAGGTGATC
    GGCTTCCTGG AAGAAGTCAT GCACGACCCG GAGCTCCTGA CCCAGGAGCG GAAGGCTGCA
    GCCAACATCA TCAGGACTCT GACCCAGGAG GACCCAGGTG ACAACCAGAT CACGCTGGAG
    GAGATCACGC AGATGGCTGA AGGCGTGAAG GCTGAGCCCT TTGAAAACCA CTCAGCCCTG
    GAGATCGCGG AGCAGCTGAC CCTGCTAGAT CACCTCGTCT TCAAGAAGAT TCCTTATGAG
    GAGTTCTTCG GACAAGGATG GATGAAACTG GAAAAGAATG AAAGGACCCC TTATATCATG
    AAAACCACTA AGCACTTCAA TGACATCAGT AACTTGATTG CTTCAGAAAT CATCCGCAAT
    GAGGACATCA ACGCCAGGGT GAGCGCCATC GAGAAGTGGG TGGCCGTAGC TGACATATGC
    CGCTGCCTCC ACAACTACAA TGCCGTACTG GAGATCACCT CGTCCATGAA CCGCAGTGCA
    ATCTTCCGGC TCAAAAAGAC GTGGCTCAAA GTCTCTAAGC AGACTAAAGC TTTGATTGAT
    AAGCTCCAAA AGCTTGTGTC ATCTGAGGGC AGATTTAAGA ATCTCAGAGA AGCTCTGAAA
    AATTGTGACC CACCCTGTGT CCCTTACCTG GGGATGTACC TCACCGACCT GGCCTTCATC
    GAGGAGGGGA CGCCCAATTA CACGGAAGAC GGCCTGGTCA ACTTCTCCAA GATGAGGATG
    ATATCCCATA TTATCCGAGA GATTCGCCAG TTTCAACAAA CTGCCTACAA AATAGAGCAC
    CAAGCAAAGG TAACGCAATA TTTACTGGAC CAATCTTTTG TAATGGATGA AGAAAGCCTC
    TACGAGTCTT CTCTCCGAAT AGAACCAAAA CTCCCCACCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002882.3
    CDS283..4104
    Translation

    Target ORF information:

    RefSeq Version NM_002891.5
    Organism Homo sapiens(human)
    Definition Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_002891.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGAGG ACCAGGGAGG GGACCAGGGA AGCAGAAATG
    AGCAGGTCCG ACGCCTCCTT ATATTGTGAT GATGTTGACA TTCGCTTCAG CAAAACCATG
    AACTCCTGCA AAGTGCTGCA GATCCGCTAC GCCAGTGTGG AGCGGCTGCT GGAGAGGCTG
    ACGGACCTGC GCTTCCTGAG CATCGACTTC CTCAACACCT TCCTGCACTC CTACCGCGTC
    TTCACCACCG CCATCGTGGT CCTGGACAAG CTCATTACCA TCTACAAGAA GCCTATCAGT
    GCCATTCCTG CCAGGTGGCT GAGGTCGCTG GAGCTCCTGT TTGCCAGTGG CCAGAACAAT
    AAGCTCCTGT ACGGTGAACC CCCCAAGTCC CCGCGCGCCA CCCGCAAGTT CTCCTCGCCG
    CCACCTCTGT CCATCACCAA GACATCGTCA CCGAGCCGCC GGCGGAAGCT CTCCCTGAAC
    ATCCCCATCA TCACTGGCGG CAAGGCCCTG GACCTGGCCG CCCTCAGCTG CAACTCCAAT
    GGCTACACCA GCATGTACTC GGCCATGTCA CCCTTCAGCA AGGCCACGCT GGACACCAGC
    AAGCTCTATG TGTCCAGCAG CTTCACCAAC AAGATTCCAG ATGAGGGCGA TACGACCCCT
    GAGAAGCCCG AAGACCCTTC AGCGCTCAGC AAGCAGAGCT CAGAAGTCTC CATGAGAGAG
    GAGTCAGATA TTGATCAAAA CCAGAGTGAT GATGGTGATA CTGAAACATC ACCAACTAAA
    TCTCCAACAA CACCCAAATC AGTCAAAAAC AAAAATTCTT CAGAGTTCCC ACTCTTTTCC
    TATAACAATG GAGTCGTCAT GACCTCCTGT CGTGAACTGG ACAATAACCG CAGTGCCTTG
    TCGGCCGCCT CTGCCTTTGC CATAGCAACC GCCGGGGCCA ACGAGGGCAC CCCAAACAAG
    GAGAAGTACC GGAGGATGTC CTTAGCCAGT GCAGGGTTTC CCCCAGACCA GAGGAATGGA
    GACAAGGAGT TTGTGATCCG CAGAGCAGCC ACCAATCGTG TCTTGAACGT GCTCCGCCAC
    TGGGTGTCCA AGCACTCTCA GGACTTTGAG ACCAACGATG AGCTCAAATG CAAGGTGATC
    GGCTTCCTGG AAGAAGTCAT GCACGACCCG GAGCTCCTGA CCCAGGAGCG GAAGGCTGCA
    GCCAACATCA TCAGGACTCT GACCCAGGAG GACCCAGGTG ACAACCAGAT CACGCTGGAG
    GAGATCACGC AGATGGCTGA AGGCGTGAAG GCTGAGCCCT TTGAAAACCA CTCAGCCCTG
    GAGATCGCGG AGCAGCTGAC CCTGCTAGAT CACCTCGTCT TCAAGAAGAT TCCTTATGAG
    GAGTTCTTCG GACAAGGATG GATGAAACTG GAAAAGAATG AAAGGACCCC TTATATCATG
    AAAACCACTA AGCACTTCAA TGACATCAGT AACTTGATTG CTTCAGAAAT CATCCGCAAT
    GAGGACATCA ACGCCAGGGT GAGCGCCATC GAGAAGTGGG TGGCCGTAGC TGACATATGC
    CGCTGCCTCC ACAACTACAA TGCCGTACTG GAGATCACCT CGTCCATGAA CCGCAGTGCA
    ATCTTCCGGC TCAAAAAGAC GTGGCTCAAA GTCTCTAAGC AGACTAAAGC TTTGATTGAT
    AAGCTCCAAA AGCTTGTGTC ATCTGAGGGC AGATTTAAGA ATCTCAGAGA AGCTCTGAAA
    AATTGTGACC CACCCTGTGT CCCTTACCTG GGGATGTACC TCACCGACCT GGCCTTCATC
    GAGGAGGGGA CGCCCAATTA CACGGAAGAC GGCCTGGTCA ACTTCTCCAA GATGAGGATG
    ATATCCCATA TTATCCGAGA GATTCGCCAG TTTCAACAAA CTGCCTACAA AATAGAGCAC
    CAAGCAAAGG TAACGCAATA TTTACTGGAC CAATCTTTTG TAATGGATGA AGAAAGCCTC
    TACGAGTCTT CTCTCCGAAT AGAACCAAAA CTCCCCACCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu08508
    Clone ID Related Accession (Same CDS sequence) NM_002891.5 , NM_002891.4
    Accession Version NM_002891.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3822bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-19
    Organism Homo sapiens(human)
    Product ras-specific guanine nucleotide-releasing factor 1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA126882.1, AK290135.1, AK226101.1, AC011944.12 and AL355696.1. On Mar 6, 2009 this sequence version replaced NM_002891.3. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.187641.1, L26584.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGAGG ACCAGGGAGG GGACCAGGGA AGCAGAAATG
    AGCAGGTCCG ACGCCTCCTT ATATTGTGAT GATGTTGACA TTCGCTTCAG CAAAACCATG
    AACTCCTGCA AAGTGCTGCA GATCCGCTAC GCCAGTGTGG AGCGGCTGCT GGAGAGGCTG
    ACGGACCTGC GCTTCCTGAG CATCGACTTC CTCAACACCT TCCTGCACTC CTACCGCGTC
    TTCACCACCG CCATCGTGGT CCTGGACAAG CTCATTACCA TCTACAAGAA GCCTATCAGT
    GCCATTCCTG CCAGGTGGCT GAGGTCGCTG GAGCTCCTGT TTGCCAGTGG CCAGAACAAT
    AAGCTCCTGT ACGGTGAACC CCCCAAGTCC CCGCGCGCCA CCCGCAAGTT CTCCTCGCCG
    CCACCTCTGT CCATCACCAA GACATCGTCA CCGAGCCGCC GGCGGAAGCT CTCCCTGAAC
    ATCCCCATCA TCACTGGCGG CAAGGCCCTG GACCTGGCCG CCCTCAGCTG CAACTCCAAT
    GGCTACACCA GCATGTACTC GGCCATGTCA CCCTTCAGCA AGGCCACGCT GGACACCAGC
    AAGCTCTATG TGTCCAGCAG CTTCACCAAC AAGATTCCAG ATGAGGGCGA TACGACCCCT
    GAGAAGCCCG AAGACCCTTC AGCGCTCAGC AAGCAGAGCT CAGAAGTCTC CATGAGAGAG
    GAGTCAGATA TTGATCAAAA CCAGAGTGAT GATGGTGATA CTGAAACATC ACCAACTAAA
    TCTCCAACAA CACCCAAATC AGTCAAAAAC AAAAATTCTT CAGAGTTCCC ACTCTTTTCC
    TATAACAATG GAGTCGTCAT GACCTCCTGT CGTGAACTGG ACAATAACCG CAGTGCCTTG
    TCGGCCGCCT CTGCCTTTGC CATAGCAACC GCCGGGGCCA ACGAGGGCAC CCCAAACAAG
    GAGAAGTACC GGAGGATGTC CTTAGCCAGT GCAGGGTTTC CCCCAGACCA GAGGAATGGA
    GACAAGGAGT TTGTGATCCG CAGAGCAGCC ACCAATCGTG TCTTGAACGT GCTCCGCCAC
    TGGGTGTCCA AGCACTCTCA GGACTTTGAG ACCAACGATG AGCTCAAATG CAAGGTGATC
    GGCTTCCTGG AAGAAGTCAT GCACGACCCG GAGCTCCTGA CCCAGGAGCG GAAGGCTGCA
    GCCAACATCA TCAGGACTCT GACCCAGGAG GACCCAGGTG ACAACCAGAT CACGCTGGAG
    GAGATCACGC AGATGGCTGA AGGCGTGAAG GCTGAGCCCT TTGAAAACCA CTCAGCCCTG
    GAGATCGCGG AGCAGCTGAC CCTGCTAGAT CACCTCGTCT TCAAGAAGAT TCCTTATGAG
    GAGTTCTTCG GACAAGGATG GATGAAACTG GAAAAGAATG AAAGGACCCC TTATATCATG
    AAAACCACTA AGCACTTCAA TGACATCAGT AACTTGATTG CTTCAGAAAT CATCCGCAAT
    GAGGACATCA ACGCCAGGGT GAGCGCCATC GAGAAGTGGG TGGCCGTAGC TGACATATGC
    CGCTGCCTCC ACAACTACAA TGCCGTACTG GAGATCACCT CGTCCATGAA CCGCAGTGCA
    ATCTTCCGGC TCAAAAAGAC GTGGCTCAAA GTCTCTAAGC AGACTAAAGC TTTGATTGAT
    AAGCTCCAAA AGCTTGTGTC ATCTGAGGGC AGATTTAAGA ATCTCAGAGA AGCTCTGAAA
    AATTGTGACC CACCCTGTGT CCCTTACCTG GGGATGTACC TCACCGACCT GGCCTTCATC
    GAGGAGGGGA CGCCCAATTA CACGGAAGAC GGCCTGGTCA ACTTCTCCAA GATGAGGATG
    ATATCCCATA TTATCCGAGA GATTCGCCAG TTTCAACAAA CTGCCTACAA AATAGAGCAC
    CAAGCAAAGG TAACGCAATA TTTACTGGAC CAATCTTTTG TAATGGATGA AGAAAGCCTC
    TACGAGTCTT CTCTCCGAAT AGAACCAAAA CTCCCCACCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002882.3
    CDS376..4197
    Translation

    Target ORF information:

    RefSeq Version NM_002891.4
    Organism Homo sapiens(human)
    Definition Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_002891.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGAGG ACCAGGGAGG GGACCAGGGA AGCAGAAATG
    AGCAGGTCCG ACGCCTCCTT ATATTGTGAT GATGTTGACA TTCGCTTCAG CAAAACCATG
    AACTCCTGCA AAGTGCTGCA GATCCGCTAC GCCAGTGTGG AGCGGCTGCT GGAGAGGCTG
    ACGGACCTGC GCTTCCTGAG CATCGACTTC CTCAACACCT TCCTGCACTC CTACCGCGTC
    TTCACCACCG CCATCGTGGT CCTGGACAAG CTCATTACCA TCTACAAGAA GCCTATCAGT
    GCCATTCCTG CCAGGTGGCT GAGGTCGCTG GAGCTCCTGT TTGCCAGTGG CCAGAACAAT
    AAGCTCCTGT ACGGTGAACC CCCCAAGTCC CCGCGCGCCA CCCGCAAGTT CTCCTCGCCG
    CCACCTCTGT CCATCACCAA GACATCGTCA CCGAGCCGCC GGCGGAAGCT CTCCCTGAAC
    ATCCCCATCA TCACTGGCGG CAAGGCCCTG GACCTGGCCG CCCTCAGCTG CAACTCCAAT
    GGCTACACCA GCATGTACTC GGCCATGTCA CCCTTCAGCA AGGCCACGCT GGACACCAGC
    AAGCTCTATG TGTCCAGCAG CTTCACCAAC AAGATTCCAG ATGAGGGCGA TACGACCCCT
    GAGAAGCCCG AAGACCCTTC AGCGCTCAGC AAGCAGAGCT CAGAAGTCTC CATGAGAGAG
    GAGTCAGATA TTGATCAAAA CCAGAGTGAT GATGGTGATA CTGAAACATC ACCAACTAAA
    TCTCCAACAA CACCCAAATC AGTCAAAAAC AAAAATTCTT CAGAGTTCCC ACTCTTTTCC
    TATAACAATG GAGTCGTCAT GACCTCCTGT CGTGAACTGG ACAATAACCG CAGTGCCTTG
    TCGGCCGCCT CTGCCTTTGC CATAGCAACC GCCGGGGCCA ACGAGGGCAC CCCAAACAAG
    GAGAAGTACC GGAGGATGTC CTTAGCCAGT GCAGGGTTTC CCCCAGACCA GAGGAATGGA
    GACAAGGAGT TTGTGATCCG CAGAGCAGCC ACCAATCGTG TCTTGAACGT GCTCCGCCAC
    TGGGTGTCCA AGCACTCTCA GGACTTTGAG ACCAACGATG AGCTCAAATG CAAGGTGATC
    GGCTTCCTGG AAGAAGTCAT GCACGACCCG GAGCTCCTGA CCCAGGAGCG GAAGGCTGCA
    GCCAACATCA TCAGGACTCT GACCCAGGAG GACCCAGGTG ACAACCAGAT CACGCTGGAG
    GAGATCACGC AGATGGCTGA AGGCGTGAAG GCTGAGCCCT TTGAAAACCA CTCAGCCCTG
    GAGATCGCGG AGCAGCTGAC CCTGCTAGAT CACCTCGTCT TCAAGAAGAT TCCTTATGAG
    GAGTTCTTCG GACAAGGATG GATGAAACTG GAAAAGAATG AAAGGACCCC TTATATCATG
    AAAACCACTA AGCACTTCAA TGACATCAGT AACTTGATTG CTTCAGAAAT CATCCGCAAT
    GAGGACATCA ACGCCAGGGT GAGCGCCATC GAGAAGTGGG TGGCCGTAGC TGACATATGC
    CGCTGCCTCC ACAACTACAA TGCCGTACTG GAGATCACCT CGTCCATGAA CCGCAGTGCA
    ATCTTCCGGC TCAAAAAGAC GTGGCTCAAA GTCTCTAAGC AGACTAAAGC TTTGATTGAT
    AAGCTCCAAA AGCTTGTGTC ATCTGAGGGC AGATTTAAGA ATCTCAGAGA AGCTCTGAAA
    AATTGTGACC CACCCTGTGT CCCTTACCTG GGGATGTACC TCACCGACCT GGCCTTCATC
    GAGGAGGGGA CGCCCAATTA CACGGAAGAC GGCCTGGTCA ACTTCTCCAA GATGAGGATG
    ATATCCCATA TTATCCGAGA GATTCGCCAG TTTCAACAAA CTGCCTACAA AATAGAGCAC
    CAAGCAAAGG TAACGCAATA TTTACTGGAC CAATCTTTTG TAATGGATGA AGAAAGCCTC
    TACGAGTCTT CTCTCCGAAT AGAACCAAAA CTCCCCACCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60207
    Clone ID Related Accession (Same CDS sequence) XM_011521866.2 , XM_011521866.3
    Accession Version XM_011521866.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3786bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ras-specific guanine nucleotide-releasing factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521866.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGAGG ACCAGGGAGG GGACCAGGGA AGCAGAAATG
    AGCAGGTCCG ACGCCTCCTT ATATTGTGAT GATGTTGACA TTCGCTTCAG CAAAACCATG
    AACTCCTGCA AAGTGCTGCA GATCCGCTAC GCCAGTGTGG AGCGGCTGCT GGAGAGGCTG
    ACGGACCTGC GCTTCCTGAG CATCGACTTC CTCAACACCT TCCTGCACTC CTACCGCGTC
    TTCACCACCG CCATCGTGGT CCTGGACAAG CTCATTACCA TCTACAAGAA GCCTATCAGT
    GCCATTCCTG CCAGGTGGCT GAGGTCGCTG GAGCTCCTGT TTGCCAGTGG CCAGAACAAT
    AAGCTCCTGT ACGGTGAACC CCCCAAGTCC CCGCGCGCCA CCCGCAAGTT CTCCTCGCCG
    CCACCTCTGT CCATCACCAA GACATCGTCA CCGAGCCGCC GGCGGAAGCT CTCCCTGAAC
    ATCCCCATCA TCACTGGCGG CAAGGCCCTG GACCTGGCCG CCCTCAGCTG CAACTCCAAT
    GGCTACACCA GCATGTACTC GGCCATGTCA CCCTTCAGCA AGGCCACGCT GGACACCAGC
    AAGCTCTATG TGTCCAGCAG CTTCACCAAC AAGATTCCAG ATGAGGGCGA TACGACCCCT
    GAGAAGCCCG AAGACCCTTC AGCGCTCAGC AAGCAGAGCT CAGAAGTCTC CATGAGAGAG
    GAGTCAGATA TTGATCAAAA CCAGAGTGAT GATGGTGATA CTGAAACATC ACCAACTAAA
    TCTCCAACAA CACCCAAATC AGTCAAAAAC AAAAATTCTT CAGAGTTCCC ACTCTTTTCC
    TATAACAATG GAGTCGTCAT GACCTCCTGT CGTGAACTGG ACAATAACCG CAGTGCCTTG
    TCGGCCGCCT CTGCCTTTGC CATAGCAACC GCCGGGGCCA ACGAGGGCAC CCCAAACAAG
    GAGAAGTACC GGAGGATGTC CTTAGCCAGT GCAGGGTTTC CCCCAGACCA GAGGAATGGA
    GACAAGGAGT TTGTGATCCG CAGAGCAGCC ACCAATCGTG TCTTGAACGT GCTCCGCCAC
    TGGGTGTCCA AGCACTCTCA GGACTTTGAG ACCAACGATG AGCTCAAATG CAAGGTGATC
    GGCTTCCTGG AAGAAGTCAT GCACGACCCG GAGCTCCTGA CCCAGGAGCG GAAGGCTGCA
    GCCAACATCA TCAGGACTCT GACCCAGGAG GACCCAGGTG ACAACCAGAT CACGCTGGAG
    GAGATCACGC AGATGGCTGA AGGCGTGAAG GCTGAGCCCT TTGAAAACCA CTCAGCCCTG
    GAGATCGCGG AGCAGCTGAC CCTGCTAGAT CACCTCGTCT TCAAGAAGAT TCCTTATGAG
    GAGTTCTTCG GACAAGGATG GATGAAACTG GAAAAGAATG AAAGGACCCC TTATATCATG
    AAAACCACTA AGCACTTCAA TGACATCAGT AACTTGATTG CTTCAGAAAT CATCCGCAAT
    GAGGACATCA ACGCCAGGGT GAGCGCCATC GAGAAGTGGG TGGCCGTAGC TGACATATGC
    CGCTGCCTCC ACAACTACAA TGCCGTACTG GAGATCACCT CGTCCATGAA CCGCAGTGCA
    ATCTTCCGGC TCAAAAAGAC GTGGCTCAAA GTCTCTAAGC AGACTAAAGC TTTGATTGAT
    AAGCTCCAAA AGCTTGTGTC ATCTGAGGGC AGATTTAAGA ATCTCAGAGA AGCTCTGAAA
    AAACAGATGT TGCACCCATC TGTGCTGTCC AGCCGTCTCT CTTCTGCCTG GGCTCCCGAG
    AGCCCCTGTG GACTGTGCTT GTGGGGAGCT GCCCCCTCCG TGCATTCACC AACTTGTCCG
    TCCGTCCGCC CCCGGGGCAC CACTCCATCC ACCTCCTCAC ATGGCTGGCT TCCTCGTCTG
    CCGCCGCCAC CACCGCTGCC TCCACTGCCT CTGGGGCCCC CCATTCTGTC TGAGTCCCCA
    CCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011520168.1
    CDS276..4061
    Translation

    Target ORF information:

    RefSeq Version XM_011521866.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011521866.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGAGG ACCAGGGAGG GGACCAGGGA AGCAGAAATG
    AGCAGGTCCG ACGCCTCCTT ATATTGTGAT GATGTTGACA TTCGCTTCAG CAAAACCATG
    AACTCCTGCA AAGTGCTGCA GATCCGCTAC GCCAGTGTGG AGCGGCTGCT GGAGAGGCTG
    ACGGACCTGC GCTTCCTGAG CATCGACTTC CTCAACACCT TCCTGCACTC CTACCGCGTC
    TTCACCACCG CCATCGTGGT CCTGGACAAG CTCATTACCA TCTACAAGAA GCCTATCAGT
    GCCATTCCTG CCAGGTGGCT GAGGTCGCTG GAGCTCCTGT TTGCCAGTGG CCAGAACAAT
    AAGCTCCTGT ACGGTGAACC CCCCAAGTCC CCGCGCGCCA CCCGCAAGTT CTCCTCGCCG
    CCACCTCTGT CCATCACCAA GACATCGTCA CCGAGCCGCC GGCGGAAGCT CTCCCTGAAC
    ATCCCCATCA TCACTGGCGG CAAGGCCCTG GACCTGGCCG CCCTCAGCTG CAACTCCAAT
    GGCTACACCA GCATGTACTC GGCCATGTCA CCCTTCAGCA AGGCCACGCT GGACACCAGC
    AAGCTCTATG TGTCCAGCAG CTTCACCAAC AAGATTCCAG ATGAGGGCGA TACGACCCCT
    GAGAAGCCCG AAGACCCTTC AGCGCTCAGC AAGCAGAGCT CAGAAGTCTC CATGAGAGAG
    GAGTCAGATA TTGATCAAAA CCAGAGTGAT GATGGTGATA CTGAAACATC ACCAACTAAA
    TCTCCAACAA CACCCAAATC AGTCAAAAAC AAAAATTCTT CAGAGTTCCC ACTCTTTTCC
    TATAACAATG GAGTCGTCAT GACCTCCTGT CGTGAACTGG ACAATAACCG CAGTGCCTTG
    TCGGCCGCCT CTGCCTTTGC CATAGCAACC GCCGGGGCCA ACGAGGGCAC CCCAAACAAG
    GAGAAGTACC GGAGGATGTC CTTAGCCAGT GCAGGGTTTC CCCCAGACCA GAGGAATGGA
    GACAAGGAGT TTGTGATCCG CAGAGCAGCC ACCAATCGTG TCTTGAACGT GCTCCGCCAC
    TGGGTGTCCA AGCACTCTCA GGACTTTGAG ACCAACGATG AGCTCAAATG CAAGGTGATC
    GGCTTCCTGG AAGAAGTCAT GCACGACCCG GAGCTCCTGA CCCAGGAGCG GAAGGCTGCA
    GCCAACATCA TCAGGACTCT GACCCAGGAG GACCCAGGTG ACAACCAGAT CACGCTGGAG
    GAGATCACGC AGATGGCTGA AGGCGTGAAG GCTGAGCCCT TTGAAAACCA CTCAGCCCTG
    GAGATCGCGG AGCAGCTGAC CCTGCTAGAT CACCTCGTCT TCAAGAAGAT TCCTTATGAG
    GAGTTCTTCG GACAAGGATG GATGAAACTG GAAAAGAATG AAAGGACCCC TTATATCATG
    AAAACCACTA AGCACTTCAA TGACATCAGT AACTTGATTG CTTCAGAAAT CATCCGCAAT
    GAGGACATCA ACGCCAGGGT GAGCGCCATC GAGAAGTGGG TGGCCGTAGC TGACATATGC
    CGCTGCCTCC ACAACTACAA TGCCGTACTG GAGATCACCT CGTCCATGAA CCGCAGTGCA
    ATCTTCCGGC TCAAAAAGAC GTGGCTCAAA GTCTCTAAGC AGACTAAAGC TTTGATTGAT
    AAGCTCCAAA AGCTTGTGTC ATCTGAGGGC AGATTTAAGA ATCTCAGAGA AGCTCTGAAA
    AAACAGATGT TGCACCCATC TGTGCTGTCC AGCCGTCTCT CTTCTGCCTG GGCTCCCGAG
    AGCCCCTGTG GACTGTGCTT GTGGGGAGCT GCCCCCTCCG TGCATTCACC AACTTGTCCG
    TCCGTCCGCC CCCGGGGCAC CACTCCATCC ACCTCCTCAC ATGGCTGGCT TCCTCGTCTG
    CCGCCGCCAC CACCGCTGCC TCCACTGCCT CTGGGGCCCC CCATTCTGTC TGAGTCCCCA
    CCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60207
    Clone ID Related Accession (Same CDS sequence) XM_011521866.2 , XM_011521866.3
    Accession Version XM_011521866.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3786bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ras-specific guanine nucleotide-releasing factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011521866.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGAGG ACCAGGGAGG GGACCAGGGA AGCAGAAATG
    AGCAGGTCCG ACGCCTCCTT ATATTGTGAT GATGTTGACA TTCGCTTCAG CAAAACCATG
    AACTCCTGCA AAGTGCTGCA GATCCGCTAC GCCAGTGTGG AGCGGCTGCT GGAGAGGCTG
    ACGGACCTGC GCTTCCTGAG CATCGACTTC CTCAACACCT TCCTGCACTC CTACCGCGTC
    TTCACCACCG CCATCGTGGT CCTGGACAAG CTCATTACCA TCTACAAGAA GCCTATCAGT
    GCCATTCCTG CCAGGTGGCT GAGGTCGCTG GAGCTCCTGT TTGCCAGTGG CCAGAACAAT
    AAGCTCCTGT ACGGTGAACC CCCCAAGTCC CCGCGCGCCA CCCGCAAGTT CTCCTCGCCG
    CCACCTCTGT CCATCACCAA GACATCGTCA CCGAGCCGCC GGCGGAAGCT CTCCCTGAAC
    ATCCCCATCA TCACTGGCGG CAAGGCCCTG GACCTGGCCG CCCTCAGCTG CAACTCCAAT
    GGCTACACCA GCATGTACTC GGCCATGTCA CCCTTCAGCA AGGCCACGCT GGACACCAGC
    AAGCTCTATG TGTCCAGCAG CTTCACCAAC AAGATTCCAG ATGAGGGCGA TACGACCCCT
    GAGAAGCCCG AAGACCCTTC AGCGCTCAGC AAGCAGAGCT CAGAAGTCTC CATGAGAGAG
    GAGTCAGATA TTGATCAAAA CCAGAGTGAT GATGGTGATA CTGAAACATC ACCAACTAAA
    TCTCCAACAA CACCCAAATC AGTCAAAAAC AAAAATTCTT CAGAGTTCCC ACTCTTTTCC
    TATAACAATG GAGTCGTCAT GACCTCCTGT CGTGAACTGG ACAATAACCG CAGTGCCTTG
    TCGGCCGCCT CTGCCTTTGC CATAGCAACC GCCGGGGCCA ACGAGGGCAC CCCAAACAAG
    GAGAAGTACC GGAGGATGTC CTTAGCCAGT GCAGGGTTTC CCCCAGACCA GAGGAATGGA
    GACAAGGAGT TTGTGATCCG CAGAGCAGCC ACCAATCGTG TCTTGAACGT GCTCCGCCAC
    TGGGTGTCCA AGCACTCTCA GGACTTTGAG ACCAACGATG AGCTCAAATG CAAGGTGATC
    GGCTTCCTGG AAGAAGTCAT GCACGACCCG GAGCTCCTGA CCCAGGAGCG GAAGGCTGCA
    GCCAACATCA TCAGGACTCT GACCCAGGAG GACCCAGGTG ACAACCAGAT CACGCTGGAG
    GAGATCACGC AGATGGCTGA AGGCGTGAAG GCTGAGCCCT TTGAAAACCA CTCAGCCCTG
    GAGATCGCGG AGCAGCTGAC CCTGCTAGAT CACCTCGTCT TCAAGAAGAT TCCTTATGAG
    GAGTTCTTCG GACAAGGATG GATGAAACTG GAAAAGAATG AAAGGACCCC TTATATCATG
    AAAACCACTA AGCACTTCAA TGACATCAGT AACTTGATTG CTTCAGAAAT CATCCGCAAT
    GAGGACATCA ACGCCAGGGT GAGCGCCATC GAGAAGTGGG TGGCCGTAGC TGACATATGC
    CGCTGCCTCC ACAACTACAA TGCCGTACTG GAGATCACCT CGTCCATGAA CCGCAGTGCA
    ATCTTCCGGC TCAAAAAGAC GTGGCTCAAA GTCTCTAAGC AGACTAAAGC TTTGATTGAT
    AAGCTCCAAA AGCTTGTGTC ATCTGAGGGC AGATTTAAGA ATCTCAGAGA AGCTCTGAAA
    AAACAGATGT TGCACCCATC TGTGCTGTCC AGCCGTCTCT CTTCTGCCTG GGCTCCCGAG
    AGCCCCTGTG GACTGTGCTT GTGGGGAGCT GCCCCCTCCG TGCATTCACC AACTTGTCCG
    TCCGTCCGCC CCCGGGGCAC CACTCCATCC ACCTCCTCAC ATGGCTGGCT TCCTCGTCTG
    CCGCCGCCAC CACCGCTGCC TCCACTGCCT CTGGGGCCCC CCATTCTGTC TGAGTCCCCA
    CCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011520168.1
    CDS275..4060
    Translation

    Target ORF information:

    RefSeq Version XM_011521866.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011521866.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGAGG ACCAGGGAGG GGACCAGGGA AGCAGAAATG
    AGCAGGTCCG ACGCCTCCTT ATATTGTGAT GATGTTGACA TTCGCTTCAG CAAAACCATG
    AACTCCTGCA AAGTGCTGCA GATCCGCTAC GCCAGTGTGG AGCGGCTGCT GGAGAGGCTG
    ACGGACCTGC GCTTCCTGAG CATCGACTTC CTCAACACCT TCCTGCACTC CTACCGCGTC
    TTCACCACCG CCATCGTGGT CCTGGACAAG CTCATTACCA TCTACAAGAA GCCTATCAGT
    GCCATTCCTG CCAGGTGGCT GAGGTCGCTG GAGCTCCTGT TTGCCAGTGG CCAGAACAAT
    AAGCTCCTGT ACGGTGAACC CCCCAAGTCC CCGCGCGCCA CCCGCAAGTT CTCCTCGCCG
    CCACCTCTGT CCATCACCAA GACATCGTCA CCGAGCCGCC GGCGGAAGCT CTCCCTGAAC
    ATCCCCATCA TCACTGGCGG CAAGGCCCTG GACCTGGCCG CCCTCAGCTG CAACTCCAAT
    GGCTACACCA GCATGTACTC GGCCATGTCA CCCTTCAGCA AGGCCACGCT GGACACCAGC
    AAGCTCTATG TGTCCAGCAG CTTCACCAAC AAGATTCCAG ATGAGGGCGA TACGACCCCT
    GAGAAGCCCG AAGACCCTTC AGCGCTCAGC AAGCAGAGCT CAGAAGTCTC CATGAGAGAG
    GAGTCAGATA TTGATCAAAA CCAGAGTGAT GATGGTGATA CTGAAACATC ACCAACTAAA
    TCTCCAACAA CACCCAAATC AGTCAAAAAC AAAAATTCTT CAGAGTTCCC ACTCTTTTCC
    TATAACAATG GAGTCGTCAT GACCTCCTGT CGTGAACTGG ACAATAACCG CAGTGCCTTG
    TCGGCCGCCT CTGCCTTTGC CATAGCAACC GCCGGGGCCA ACGAGGGCAC CCCAAACAAG
    GAGAAGTACC GGAGGATGTC CTTAGCCAGT GCAGGGTTTC CCCCAGACCA GAGGAATGGA
    GACAAGGAGT TTGTGATCCG CAGAGCAGCC ACCAATCGTG TCTTGAACGT GCTCCGCCAC
    TGGGTGTCCA AGCACTCTCA GGACTTTGAG ACCAACGATG AGCTCAAATG CAAGGTGATC
    GGCTTCCTGG AAGAAGTCAT GCACGACCCG GAGCTCCTGA CCCAGGAGCG GAAGGCTGCA
    GCCAACATCA TCAGGACTCT GACCCAGGAG GACCCAGGTG ACAACCAGAT CACGCTGGAG
    GAGATCACGC AGATGGCTGA AGGCGTGAAG GCTGAGCCCT TTGAAAACCA CTCAGCCCTG
    GAGATCGCGG AGCAGCTGAC CCTGCTAGAT CACCTCGTCT TCAAGAAGAT TCCTTATGAG
    GAGTTCTTCG GACAAGGATG GATGAAACTG GAAAAGAATG AAAGGACCCC TTATATCATG
    AAAACCACTA AGCACTTCAA TGACATCAGT AACTTGATTG CTTCAGAAAT CATCCGCAAT
    GAGGACATCA ACGCCAGGGT GAGCGCCATC GAGAAGTGGG TGGCCGTAGC TGACATATGC
    CGCTGCCTCC ACAACTACAA TGCCGTACTG GAGATCACCT CGTCCATGAA CCGCAGTGCA
    ATCTTCCGGC TCAAAAAGAC GTGGCTCAAA GTCTCTAAGC AGACTAAAGC TTTGATTGAT
    AAGCTCCAAA AGCTTGTGTC ATCTGAGGGC AGATTTAAGA ATCTCAGAGA AGCTCTGAAA
    AAACAGATGT TGCACCCATC TGTGCTGTCC AGCCGTCTCT CTTCTGCCTG GGCTCCCGAG
    AGCCCCTGTG GACTGTGCTT GTGGGGAGCT GCCCCCTCCG TGCATTCACC AACTTGTCCG
    TCCGTCCGCC CCCGGGGCAC CACTCCATCC ACCTCCTCAC ATGGCTGGCT TCCTCGTCTG
    CCGCCGCCAC CACCGCTGCC TCCACTGCCT CTGGGGCCCC CCATTCTGTC TGAGTCCCCA
    CCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60208
    Clone ID Related Accession (Same CDS sequence) XM_011521867.2 , XM_011521867.3
    Accession Version XM_011521867.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3597bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ras-specific guanine nucleotide-releasing factor 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521867.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGAGG ACCAGGGAGG GGACCAGGGA AGCAGAAATG
    AGCAGGTCCG ACGCCTCCTT ATATTGTGAT GATGTTGACA TTCGCTTCAG CAAAACCATG
    AACTCCTGCA AAGTGCTGCA GATCCGCTAC GCCAGTGTGG AGCGGCTGCT GGAGAGGCTG
    ACGGACCTGC GCTTCCTGAG CATCGACTTC CTCAACACCT TCCTGCACTC CTACCGCGTC
    TTCACCACCG CCATCGTGGT CCTGGACAAG CTCATTACCA TCTACAAGAA GCCTATCAGT
    GCCATTCCTG CCAGGTGGCT GAGGTCGCTG GAGCTCCTGT TTGCCAGTGG CCAGAACAAT
    AAGCTCCTGT ACGGTGAACC CCCCAAGTCC CCGCGCGCCA CCCGCAAGTT CTCCTCGCCG
    CCACCTCTGT CCATCACCAA GACATCGTCA CCGAGCCGCC GGCGGAAGCT CTCCCTGAAC
    ATCCCCATCA TCACTGGCGG CAAGGCCCTG GACCTGGCCG CCCTCAGCTG CAACTCCAAT
    GGCTACACCA GCATGTACTC GGCCATGTCA CCCTTCAGCA AGGCCACGCT GGACACCAGC
    AAGCTCTATG TGTCCAGCAG CTTCACCAAC AAGATTCCAG ATGAGGGCGA TACGACCCCT
    GAGAAGCCCG AAGACCCTTC AGCGCTCAGC AAGCAGAGCT CAGAAGTCTC CATGAGAGAG
    GAGTCAGATA TTGATCAAAA CCAGAGTGAT GATGGTGATA CTGAAACATC ACCAACTAAA
    TCTCCAACAA CACCCAAATC AGTCAAAAAC AAAAATTCTT CAGAGTTCCC ACTCTTTTCC
    TATAACAATG GAGTCGTCAT GACCTCCTGT CGTGAACTGG ACAATAACCG CAGTGCCTTG
    TCGGCCGCCT CTGCCTTTGC CATAGCAACC GCCGGGGCCA ACGAGGGCAC CCCAAACAAG
    GAGAAGTACC GGAGGATGTC CTTAGCCAGT GCAGGGTTTC CCCCAGACCA GAGGAATGGA
    GACAAGGAGT TTGTGATCCG CAGAGCAGCC ACCAATCGTG TCTTGAACGT GCTCCGCCAC
    TGGGTGTCCA AGCACTCTCA GGACTTTGAG ACCAACGATG AGCTCAAATG CAAGGTGATC
    GGCTTCCTGG AAGAAGTCAT GCACGACCCG GAGCTCCTGA CCCAGGAGCG GAAGGCTGCA
    GCCAACATCA TCAGGACTCT GACCCAGGAG GACCCAGGTG ACAACCAGAT CACGCTGGAG
    GAGATCACGC AGATGGCTGA AGGCGTGAAG GCTGAGCCCT TTGAAAACCA CTCAGCCCTG
    GAGATCGCGG AGCAGCTGAC CCTGCTAGAT CACCTCGTCT TCAAGAAGAT TCCTTATGAG
    GAGTTCTTCG GACAAGGATG GATGAAACTG GAAAAGAATG AAAGGACCCC TTATATCATG
    AAAACCACTA AGCACTTCAA TGACATCAGT AACTTGATTG CTTCAGAAAT CATCCGCAAT
    GAGGACATCA ACGCCAGGGT GAGCGCCATC GAGAAGTGGG TGGCCGTAGC TGACATATGC
    CGCTGCCTCC ACAACTACAA TGCCGTACTG GAGATCACCT CGTCCATGAA CCGCAGTGCA
    ATCTTCCGGC TCAAAAAGAC GTGGCTCAAA GTCTCTAAGC AGACTAAAGC TTTGATTGAT
    AAGCTCCAAA AGCTTGTGTC ATCTGAGGGC AGATTTAAGA ATCTCAGAGA AGCTCTGAAA
    AAATATCCCA TATTATCCGA GAGATTCGCC AGTTTCAACA AACTGCCTAC AAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011520169.1
    CDS276..3872
    Translation

    Target ORF information:

    RefSeq Version XM_011521867.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011521867.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGAGG ACCAGGGAGG GGACCAGGGA AGCAGAAATG
    AGCAGGTCCG ACGCCTCCTT ATATTGTGAT GATGTTGACA TTCGCTTCAG CAAAACCATG
    AACTCCTGCA AAGTGCTGCA GATCCGCTAC GCCAGTGTGG AGCGGCTGCT GGAGAGGCTG
    ACGGACCTGC GCTTCCTGAG CATCGACTTC CTCAACACCT TCCTGCACTC CTACCGCGTC
    TTCACCACCG CCATCGTGGT CCTGGACAAG CTCATTACCA TCTACAAGAA GCCTATCAGT
    GCCATTCCTG CCAGGTGGCT GAGGTCGCTG GAGCTCCTGT TTGCCAGTGG CCAGAACAAT
    AAGCTCCTGT ACGGTGAACC CCCCAAGTCC CCGCGCGCCA CCCGCAAGTT CTCCTCGCCG
    CCACCTCTGT CCATCACCAA GACATCGTCA CCGAGCCGCC GGCGGAAGCT CTCCCTGAAC
    ATCCCCATCA TCACTGGCGG CAAGGCCCTG GACCTGGCCG CCCTCAGCTG CAACTCCAAT
    GGCTACACCA GCATGTACTC GGCCATGTCA CCCTTCAGCA AGGCCACGCT GGACACCAGC
    AAGCTCTATG TGTCCAGCAG CTTCACCAAC AAGATTCCAG ATGAGGGCGA TACGACCCCT
    GAGAAGCCCG AAGACCCTTC AGCGCTCAGC AAGCAGAGCT CAGAAGTCTC CATGAGAGAG
    GAGTCAGATA TTGATCAAAA CCAGAGTGAT GATGGTGATA CTGAAACATC ACCAACTAAA
    TCTCCAACAA CACCCAAATC AGTCAAAAAC AAAAATTCTT CAGAGTTCCC ACTCTTTTCC
    TATAACAATG GAGTCGTCAT GACCTCCTGT CGTGAACTGG ACAATAACCG CAGTGCCTTG
    TCGGCCGCCT CTGCCTTTGC CATAGCAACC GCCGGGGCCA ACGAGGGCAC CCCAAACAAG
    GAGAAGTACC GGAGGATGTC CTTAGCCAGT GCAGGGTTTC CCCCAGACCA GAGGAATGGA
    GACAAGGAGT TTGTGATCCG CAGAGCAGCC ACCAATCGTG TCTTGAACGT GCTCCGCCAC
    TGGGTGTCCA AGCACTCTCA GGACTTTGAG ACCAACGATG AGCTCAAATG CAAGGTGATC
    GGCTTCCTGG AAGAAGTCAT GCACGACCCG GAGCTCCTGA CCCAGGAGCG GAAGGCTGCA
    GCCAACATCA TCAGGACTCT GACCCAGGAG GACCCAGGTG ACAACCAGAT CACGCTGGAG
    GAGATCACGC AGATGGCTGA AGGCGTGAAG GCTGAGCCCT TTGAAAACCA CTCAGCCCTG
    GAGATCGCGG AGCAGCTGAC CCTGCTAGAT CACCTCGTCT TCAAGAAGAT TCCTTATGAG
    GAGTTCTTCG GACAAGGATG GATGAAACTG GAAAAGAATG AAAGGACCCC TTATATCATG
    AAAACCACTA AGCACTTCAA TGACATCAGT AACTTGATTG CTTCAGAAAT CATCCGCAAT
    GAGGACATCA ACGCCAGGGT GAGCGCCATC GAGAAGTGGG TGGCCGTAGC TGACATATGC
    CGCTGCCTCC ACAACTACAA TGCCGTACTG GAGATCACCT CGTCCATGAA CCGCAGTGCA
    ATCTTCCGGC TCAAAAAGAC GTGGCTCAAA GTCTCTAAGC AGACTAAAGC TTTGATTGAT
    AAGCTCCAAA AGCTTGTGTC ATCTGAGGGC AGATTTAAGA ATCTCAGAGA AGCTCTGAAA
    AAATATCCCA TATTATCCGA GAGATTCGCC AGTTTCAACA AACTGCCTAC AAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60208
    Clone ID Related Accession (Same CDS sequence) XM_011521867.2 , XM_011521867.3
    Accession Version XM_011521867.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3597bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ras-specific guanine nucleotide-releasing factor 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011521867.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGAGG ACCAGGGAGG GGACCAGGGA AGCAGAAATG
    AGCAGGTCCG ACGCCTCCTT ATATTGTGAT GATGTTGACA TTCGCTTCAG CAAAACCATG
    AACTCCTGCA AAGTGCTGCA GATCCGCTAC GCCAGTGTGG AGCGGCTGCT GGAGAGGCTG
    ACGGACCTGC GCTTCCTGAG CATCGACTTC CTCAACACCT TCCTGCACTC CTACCGCGTC
    TTCACCACCG CCATCGTGGT CCTGGACAAG CTCATTACCA TCTACAAGAA GCCTATCAGT
    GCCATTCCTG CCAGGTGGCT GAGGTCGCTG GAGCTCCTGT TTGCCAGTGG CCAGAACAAT
    AAGCTCCTGT ACGGTGAACC CCCCAAGTCC CCGCGCGCCA CCCGCAAGTT CTCCTCGCCG
    CCACCTCTGT CCATCACCAA GACATCGTCA CCGAGCCGCC GGCGGAAGCT CTCCCTGAAC
    ATCCCCATCA TCACTGGCGG CAAGGCCCTG GACCTGGCCG CCCTCAGCTG CAACTCCAAT
    GGCTACACCA GCATGTACTC GGCCATGTCA CCCTTCAGCA AGGCCACGCT GGACACCAGC
    AAGCTCTATG TGTCCAGCAG CTTCACCAAC AAGATTCCAG ATGAGGGCGA TACGACCCCT
    GAGAAGCCCG AAGACCCTTC AGCGCTCAGC AAGCAGAGCT CAGAAGTCTC CATGAGAGAG
    GAGTCAGATA TTGATCAAAA CCAGAGTGAT GATGGTGATA CTGAAACATC ACCAACTAAA
    TCTCCAACAA CACCCAAATC AGTCAAAAAC AAAAATTCTT CAGAGTTCCC ACTCTTTTCC
    TATAACAATG GAGTCGTCAT GACCTCCTGT CGTGAACTGG ACAATAACCG CAGTGCCTTG
    TCGGCCGCCT CTGCCTTTGC CATAGCAACC GCCGGGGCCA ACGAGGGCAC CCCAAACAAG
    GAGAAGTACC GGAGGATGTC CTTAGCCAGT GCAGGGTTTC CCCCAGACCA GAGGAATGGA
    GACAAGGAGT TTGTGATCCG CAGAGCAGCC ACCAATCGTG TCTTGAACGT GCTCCGCCAC
    TGGGTGTCCA AGCACTCTCA GGACTTTGAG ACCAACGATG AGCTCAAATG CAAGGTGATC
    GGCTTCCTGG AAGAAGTCAT GCACGACCCG GAGCTCCTGA CCCAGGAGCG GAAGGCTGCA
    GCCAACATCA TCAGGACTCT GACCCAGGAG GACCCAGGTG ACAACCAGAT CACGCTGGAG
    GAGATCACGC AGATGGCTGA AGGCGTGAAG GCTGAGCCCT TTGAAAACCA CTCAGCCCTG
    GAGATCGCGG AGCAGCTGAC CCTGCTAGAT CACCTCGTCT TCAAGAAGAT TCCTTATGAG
    GAGTTCTTCG GACAAGGATG GATGAAACTG GAAAAGAATG AAAGGACCCC TTATATCATG
    AAAACCACTA AGCACTTCAA TGACATCAGT AACTTGATTG CTTCAGAAAT CATCCGCAAT
    GAGGACATCA ACGCCAGGGT GAGCGCCATC GAGAAGTGGG TGGCCGTAGC TGACATATGC
    CGCTGCCTCC ACAACTACAA TGCCGTACTG GAGATCACCT CGTCCATGAA CCGCAGTGCA
    ATCTTCCGGC TCAAAAAGAC GTGGCTCAAA GTCTCTAAGC AGACTAAAGC TTTGATTGAT
    AAGCTCCAAA AGCTTGTGTC ATCTGAGGGC AGATTTAAGA ATCTCAGAGA AGCTCTGAAA
    AAATATCCCA TATTATCCGA GAGATTCGCC AGTTTCAACA AACTGCCTAC AAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011520169.1
    CDS275..3871
    Translation

    Target ORF information:

    RefSeq Version XM_011521867.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011521867.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
    AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA
    ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG
    CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG
    CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC
    CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG
    GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG
    CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACAGAGA AGACCGTGGC CAAGCAGCTT
    CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCACATCC
    CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC
    AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG
    AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG
    AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC
    ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC
    ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT
    CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT
    GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC
    CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG
    AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC
    CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT
    CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC
    AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG
    CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA
    GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG
    TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT
    ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC
    ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC
    CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT
    ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC
    CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC
    AAGGTCACTG TGCCGCAGAT GATCAAGAGG ACCAGGGAGG GGACCAGGGA AGCAGAAATG
    AGCAGGTCCG ACGCCTCCTT ATATTGTGAT GATGTTGACA TTCGCTTCAG CAAAACCATG
    AACTCCTGCA AAGTGCTGCA GATCCGCTAC GCCAGTGTGG AGCGGCTGCT GGAGAGGCTG
    ACGGACCTGC GCTTCCTGAG CATCGACTTC CTCAACACCT TCCTGCACTC CTACCGCGTC
    TTCACCACCG CCATCGTGGT CCTGGACAAG CTCATTACCA TCTACAAGAA GCCTATCAGT
    GCCATTCCTG CCAGGTGGCT GAGGTCGCTG GAGCTCCTGT TTGCCAGTGG CCAGAACAAT
    AAGCTCCTGT ACGGTGAACC CCCCAAGTCC CCGCGCGCCA CCCGCAAGTT CTCCTCGCCG
    CCACCTCTGT CCATCACCAA GACATCGTCA CCGAGCCGCC GGCGGAAGCT CTCCCTGAAC
    ATCCCCATCA TCACTGGCGG CAAGGCCCTG GACCTGGCCG CCCTCAGCTG CAACTCCAAT
    GGCTACACCA GCATGTACTC GGCCATGTCA CCCTTCAGCA AGGCCACGCT GGACACCAGC
    AAGCTCTATG TGTCCAGCAG CTTCACCAAC AAGATTCCAG ATGAGGGCGA TACGACCCCT
    GAGAAGCCCG AAGACCCTTC AGCGCTCAGC AAGCAGAGCT CAGAAGTCTC CATGAGAGAG
    GAGTCAGATA TTGATCAAAA CCAGAGTGAT GATGGTGATA CTGAAACATC ACCAACTAAA
    TCTCCAACAA CACCCAAATC AGTCAAAAAC AAAAATTCTT CAGAGTTCCC ACTCTTTTCC
    TATAACAATG GAGTCGTCAT GACCTCCTGT CGTGAACTGG ACAATAACCG CAGTGCCTTG
    TCGGCCGCCT CTGCCTTTGC CATAGCAACC GCCGGGGCCA ACGAGGGCAC CCCAAACAAG
    GAGAAGTACC GGAGGATGTC CTTAGCCAGT GCAGGGTTTC CCCCAGACCA GAGGAATGGA
    GACAAGGAGT TTGTGATCCG CAGAGCAGCC ACCAATCGTG TCTTGAACGT GCTCCGCCAC
    TGGGTGTCCA AGCACTCTCA GGACTTTGAG ACCAACGATG AGCTCAAATG CAAGGTGATC
    GGCTTCCTGG AAGAAGTCAT GCACGACCCG GAGCTCCTGA CCCAGGAGCG GAAGGCTGCA
    GCCAACATCA TCAGGACTCT GACCCAGGAG GACCCAGGTG ACAACCAGAT CACGCTGGAG
    GAGATCACGC AGATGGCTGA AGGCGTGAAG GCTGAGCCCT TTGAAAACCA CTCAGCCCTG
    GAGATCGCGG AGCAGCTGAC CCTGCTAGAT CACCTCGTCT TCAAGAAGAT TCCTTATGAG
    GAGTTCTTCG GACAAGGATG GATGAAACTG GAAAAGAATG AAAGGACCCC TTATATCATG
    AAAACCACTA AGCACTTCAA TGACATCAGT AACTTGATTG CTTCAGAAAT CATCCGCAAT
    GAGGACATCA ACGCCAGGGT GAGCGCCATC GAGAAGTGGG TGGCCGTAGC TGACATATGC
    CGCTGCCTCC ACAACTACAA TGCCGTACTG GAGATCACCT CGTCCATGAA CCGCAGTGCA
    ATCTTCCGGC TCAAAAAGAC GTGGCTCAAA GTCTCTAAGC AGACTAAAGC TTTGATTGAT
    AAGCTCCAAA AGCTTGTGTC ATCTGAGGGC AGATTTAAGA ATCTCAGAGA AGCTCTGAAA
    AAATATCCCA TATTATCCGA GAGATTCGCC AGTTTCAACA AACTGCCTAC AAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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