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RCN2 cDNA ORF clone, Homo sapiens (human)

Gene Symbol RCN2
Entrez Gene ID 5955
Full Name reticulocalbin 2, EF-hand calcium binding domain
Synonyms E6BP, ERC-55, ERC55, TCBP49
General protein information
Preferred Names
reticulocalbin-2
Names
reticulocalbin-2
E6-binding protein
calcium-binding protein ERC-55
reticulocalbin 2, EF-hand calcium binding domain (endoplasmic reticulum calcium-binding protein, 55kD)
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

15

15q23

Summary The protein encoded by this gene is a calcium-binding protein located in the lumen of the ER. The protein contains six conserved regions with similarity to a high affinity Ca(+2)-binding motif, the EF-hand. This gene maps to the same region as type 4 Bardet-Biedl syndrome, suggesting a possible causative role for this gene in the disorder. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2012]. lac of sum
Disorder MIM:

602584


mRNA and Protein(s)

mRNA Protein Name
NM_001271837 NP_001258766 reticulocalbin-2 isoform b precursor
NM_002902 NP_002893 reticulocalbin-2 isoform a precursor


Homo sapiens (human) RCN2 NP_001258766.1
Pan troglodytes (chimpanzee) RCN2 XP_003314843.1
Macaca mulatta (Rhesus monkey) RCN2 XP_001105360.2
Canis lupus familiaris (dog) RCN2 XP_544790.2
Bos taurus (cattle) RCN2 NP_001069047.1
Mus musculus (house mouse) Rcn2 NP_036122.2
Rattus norvegicus (Norway rat) Rcn2 NP_058828.2
Gallus gallus (chicken) RCN2 XP_004943770.1
Danio rerio (zebrafish) rcn2 NP_001025434.1
Drosophila melanogaster (fruit fly) CG31650 NP_723049.1
Xenopus (Silurana) tropicalis (western clawed frog) rcn2 NP_001116488.1


Related articles in PubMed

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RCN2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RCN2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu00150 NM_001271837 Homo sapiens reticulocalbin 2, EF-hand calcium binding domain (RCN2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $223.30
$319.00
OHu31757 NM_002902 Homo sapiens reticulocalbin 2, EF-hand calcium binding domain (RCN2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $69.30
$99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu00150
Clone ID Related Accession (Same CDS sequence) NM_001271837
Accession Version NM_001271837.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1008bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product reticulocalbin-2 isoform b precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB452318.1, BP275421.1, AV711990.1, AK313833.1 and AC051643.5. Transcript Variant: This variant (2) has an additional in-frame exon in the coding region, compared to variant 1. The resulting isoform (b) is longer than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal ##RefSeq-Attributes-END## ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGCGGCTGG GCCCGAGGAC CGCGGCGTTG GGGCTGCTGC TGCTGTGCGC CGCCGCGGCC 
GGCGCCGGCA AGGCCGAGGA GCTGCACTAC CCGCTGGGCG AGCGCCGCAG CGACTACGAC
CGCGAGGCGC TGCTGGGCGT CCAGGAAGAT GTGGATGAAT ATGTTAAACT CGGCCACGAA
GAGCAGCAAA AAAGACTGCA GGCGATCATA AAGAAAATCG ACTTGGACTC AGATGGCTTT
CTCACTGAAA GTGAACTCAG TTCATGGATT CAGATGTCTT TTAAGCATTA TGCTATGCAA
GAAGCAAAAC AACAGTTTGT TGAATATGAT AAAAACAGTG ATGATACTGT GACTTGGGAT
GAATATAACA TTCAGATGTA TGATCGTGTG ATTGACTTTG ATGAGAACAC TGCTCTGGAT
GATGCAGAAG AGGAGTCCTT TAGGAAGGAA TTTGCCATTT GTAAAAAACA GTCTTTCTGT
TTTTGGCTTC TTCGATTTAA TCTTCACTTA AAGGACAAGA AGCGATTTGA AAAAGCTAAC
CAGGATTCAG GTCCCGGTTT GAGTCTTGAA GAATTTATTG CTTTTGAGCA TCCTGAAGAA
GTTGATTATA TGACGGAATT TGTCATTCAA GAAGCTTTAG AAGAACATGA CAAAAATGGT
GATGGATTTG TTAGTTTGGA AGAATTTCTT GGTGATTACA GGTGGGATCC AACTGCAAAT
GAAGATCCAG AATGGATACT TGTTGAGAAA GACAGATTCG TGAATGATTA TGACAAAGAT
AACGATGGCA GGCTTGATCC CCAAGAGCTG TTACCTTGGG TAGTACCTAA TAATCAGGGC
ATTGCACAAG AGGAGGCGCT TCATCTAATT GATGAAATGG ATTTGAATGG TGACAAAAAG
CTCTCTGAAG AAGAGATTCT GGAAAACCCG GACTTGTTTC TCACCAGTGA AGCCACAGAT
TATGGCAGAC AGCTCCATGA TGACTATTTC TATCATGATG AGCTTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001258766.1
CDS222..1229
Misc Feature(1)414..584(+)
Misc Feature(2)441..584(+)
Misc Feature(3)846..1043(+)
Misc Feature(4)870..1028(+)
Misc Feature(5)984..1145(+)
Misc Feature(6)990..1145(+)
Misc Feature(7)993..1136(+)
Exon (1)1..365
Gene:RCN2
Gene Synonym:
Exon (2)366..471
Gene:RCN2
Gene Synonym:
Exon (3)472..668
Gene:RCN2
Gene Synonym:
Exon (4)669..722
Gene:RCN2
Gene Synonym:
Exon (5)723..836
Gene:RCN2
Gene Synonym:
Exon (6)837..933
Gene:RCN2
Gene Synonym:
Exon (7)934..1076
Gene:RCN2
Gene Synonym:
Exon (8)1077..2267
Gene:RCN2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001271837
Organism Homo sapiens (human)
Definition Homo sapiens reticulocalbin 2, EF-hand calcium binding domain (RCN2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001271837

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGCGGCTGG GCCCGAGGAC CGCGGCGTTG GGGCTGCTGC TGCTGTGCGC CGCCGCGGCC 
GGCGCCGGCA AGGCCGAGGA GCTGCACTAC CCGCTGGGCG AGCGCCGCAG CGACTACGAC
CGCGAGGCGC TGCTGGGCGT CCAGGAAGAT GTGGATGAAT ATGTTAAACT CGGCCACGAA
GAGCAGCAAA AAAGACTGCA GGCGATCATA AAGAAAATCG ACTTGGACTC AGATGGCTTT
CTCACTGAAA GTGAACTCAG TTCATGGATT CAGATGTCTT TTAAGCATTA TGCTATGCAA
GAAGCAAAAC AACAGTTTGT TGAATATGAT AAAAACAGTG ATGATACTGT GACTTGGGAT
GAATATAACA TTCAGATGTA TGATCGTGTG ATTGACTTTG ATGAGAACAC TGCTCTGGAT
GATGCAGAAG AGGAGTCCTT TAGGAAGGAA TTTGCCATTT GTAAAAAACA GTCTTTCTGT
TTTTGGCTTC TTCGATTTAA TCTTCACTTA AAGGACAAGA AGCGATTTGA AAAAGCTAAC
CAGGATTCAG GTCCCGGTTT GAGTCTTGAA GAATTTATTG CTTTTGAGCA TCCTGAAGAA
GTTGATTATA TGACGGAATT TGTCATTCAA GAAGCTTTAG AAGAACATGA CAAAAATGGT
GATGGATTTG TTAGTTTGGA AGAATTTCTT GGTGATTACA GGTGGGATCC AACTGCAAAT
GAAGATCCAG AATGGATACT TGTTGAGAAA GACAGATTCG TGAATGATTA TGACAAAGAT
AACGATGGCA GGCTTGATCC CCAAGAGCTG TTACCTTGGG TAGTACCTAA TAATCAGGGC
ATTGCACAAG AGGAGGCGCT TCATCTAATT GATGAAATGG ATTTGAATGG TGACAAAAAG
CTCTCTGAAG AAGAGATTCT GGAAAACCCG GACTTGTTTC TCACCAGTGA AGCCACAGAT
TATGGCAGAC AGCTCCATGA TGACTATTTC TATCATGATG AGCTTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu31757
Clone ID Related Accession (Same CDS sequence) NM_002902
Accession Version NM_002902.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 954bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product reticulocalbin-2 isoform a precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB452318.1, AK313833.1 and AC051643.5. On Feb 10, 2009 this sequence version replaced gi:4506456. Transcript Variant: This variant (1) is a predominant transcript and encodes isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: X78669.1, BC004892.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCGGCTGG GCCCGAGGAC CGCGGCGTTG GGGCTGCTGC TGCTGTGCGC CGCCGCGGCC 
GGCGCCGGCA AGGCCGAGGA GCTGCACTAC CCGCTGGGCG AGCGCCGCAG CGACTACGAC
CGCGAGGCGC TGCTGGGCGT CCAGGAAGAT GTGGATGAAT ATGTTAAACT CGGCCACGAA
GAGCAGCAAA AAAGACTGCA GGCGATCATA AAGAAAATCG ACTTGGACTC AGATGGCTTT
CTCACTGAAA GTGAACTCAG TTCATGGATT CAGATGTCTT TTAAGCATTA TGCTATGCAA
GAAGCAAAAC AACAGTTTGT TGAATATGAT AAAAACAGTG ATGATACTGT GACTTGGGAT
GAATATAACA TTCAGATGTA TGATCGTGTG ATTGACTTTG ATGAGAACAC TGCTCTGGAT
GATGCAGAAG AGGAGTCCTT TAGGAAGCTT CACTTAAAGG ACAAGAAGCG ATTTGAAAAA
GCTAACCAGG ATTCAGGTCC CGGTTTGAGT CTTGAAGAAT TTATTGCTTT TGAGCATCCT
GAAGAAGTTG ATTATATGAC GGAATTTGTC ATTCAAGAAG CTTTAGAAGA ACATGACAAA
AATGGTGATG GATTTGTTAG TTTGGAAGAA TTTCTTGGTG ATTACAGGTG GGATCCAACT
GCAAATGAAG ATCCAGAATG GATACTTGTT GAGAAAGACA GATTCGTGAA TGATTATGAC
AAAGATAACG ATGGCAGGCT TGATCCCCAA GAGCTGTTAC CTTGGGTAGT ACCTAATAAT
CAGGGCATTG CACAAGAGGA GGCGCTTCAT CTAATTGATG AAATGGATTT GAATGGTGAC
AAAAAGCTCT CTGAAGAAGA GATTCTGGAA AACCCGGACT TGTTTCTCAC CAGTGAAGCC
ACAGATTATG GCAGACAGCT CCATGATGAC TATTTCTATC ATGATGAGCT TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002893.1
CDS222..1175
Misc Feature(1)414..584(+)
Misc Feature(2)441..584(+)
Misc Feature(3)792..989(+)
Misc Feature(4)816..974(+)
Misc Feature(5)930..1091(+)
Misc Feature(6)936..1091(+)
Misc Feature(7)939..1082(+)
Misc Feature(8)1161..1172(+)
Exon (1)1..365
Gene:RCN2
Gene Synonym:
Exon (2)366..471
Gene:RCN2
Gene Synonym:
Exon (3)472..668
Gene:RCN2
Gene Synonym:
Exon (4)669..782
Gene:RCN2
Gene Synonym:
Exon (5)783..879
Gene:RCN2
Gene Synonym:
Exon (6)880..1022
Gene:RCN2
Gene Synonym:
Exon (7)1023..2213
Gene:RCN2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_002902
Organism Homo sapiens (human)
Definition Homo sapiens reticulocalbin 2, EF-hand calcium binding domain (RCN2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002902

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCGGCTGG GCCCGAGGAC CGCGGCGTTG GGGCTGCTGC TGCTGTGCGC CGCCGCGGCC 
GGCGCCGGCA AGGCCGAGGA GCTGCACTAC CCGCTGGGCG AGCGCCGCAG CGACTACGAC
CGCGAGGCGC TGCTGGGCGT CCAGGAAGAT GTGGATGAAT ATGTTAAACT CGGCCACGAA
GAGCAGCAAA AAAGACTGCA GGCGATCATA AAGAAAATCG ACTTGGACTC AGATGGCTTT
CTCACTGAAA GTGAACTCAG TTCATGGATT CAGATGTCTT TTAAGCATTA TGCTATGCAA
GAAGCAAAAC AACAGTTTGT TGAATATGAT AAAAACAGTG ATGATACTGT GACTTGGGAT
GAATATAACA TTCAGATGTA TGATCGTGTG ATTGACTTTG ATGAGAACAC TGCTCTGGAT
GATGCAGAAG AGGAGTCCTT TAGGAAGCTT CACTTAAAGG ACAAGAAGCG ATTTGAAAAA
GCTAACCAGG ATTCAGGTCC CGGTTTGAGT CTTGAAGAAT TTATTGCTTT TGAGCATCCT
GAAGAAGTTG ATTATATGAC GGAATTTGTC ATTCAAGAAG CTTTAGAAGA ACATGACAAA
AATGGTGATG GATTTGTTAG TTTGGAAGAA TTTCTTGGTG ATTACAGGTG GGATCCAACT
GCAAATGAAG ATCCAGAATG GATACTTGTT GAGAAAGACA GATTCGTGAA TGATTATGAC
AAAGATAACG ATGGCAGGCT TGATCCCCAA GAGCTGTTAC CTTGGGTAGT ACCTAATAAT
CAGGGCATTG CACAAGAGGA GGCGCTTCAT CTAATTGATG AAATGGATTT GAATGGTGAC
AAAAAGCTCT CTGAAGAAGA GATTCTGGAA AACCCGGACT TGTTTCTCAC CAGTGAAGCC
ACAGATTATG GCAGACAGCT CCATGATGAC TATTTCTATC ATGATGAGCT TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

An integrative method for scoring candidate genes from association studies: application to warfarin dosing
BMC Bioinformatics 11 (SUPPL 9), S9 (2010)
Tatonetti NP, Dudley JT, Sagreiya H, Butte AJ and Altman RB.


book

Identification and characterization of novel ERC-55 interacting proteins: evidence for the existence of several ERC-55 splicing variants; including the cytosolic ERC-55-C
Proteomics 9 (23), 5267-5287 (2009)
Ludvigsen M, Jacobsen C, Maunsbach AB and Honore B.


book

Gene expression analysis in absence epilepsy using a monozygotic twin design
Epilepsia 49 (9), 1546-1554 (2008)
Helbig I, Matigian NA, Vadlamudi L, Lawrence KM, Bayly MA, Bain SM, Diyagama D, Scheffer IE, Mulley JC, Holloway AJ, Dibbens LM, Berkovic SF and Hayward NK.


book

hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes
Genomics 89 (3), 307-315 (2007)
Lamesch P, Li N, Milstein S, Fan C, Hao T, Szabo G, Hu Z, Venkatesan K, Bethel G, Martin P, Rogers J, Lawlor S, McLaren S, Dricot A, Borick H, Cusick ME, Vandenhaute J, Dunham I, Hill DE and Vidal M.


book

Biochemical interactions of the neuronal pentraxins. Neuronal pentraxin (NP) receptor binds to taipoxin and taipoxin-associated calcium-binding protein 49 via NP1 and NP2
J. Biol. Chem. 275 (23), 17786-17792 (2000)
Kirkpatrick LL, Matzuk MM, Dodds DC and Perin MS.


book

Neuronal pentraxin receptor, a novel putative integral membrane pentraxin that interacts with neuronal pentraxin 1 and 2 and taipoxin-associated calcium-binding protein 49
J. Biol. Chem. 272 (34), 21488-21494 (1997)
Dodds DC, Omeis IA, Cushman SJ, Helms JA and Perin MS.


book

Assignment of the gene for ERC-55 (RCN2) to human chromosome band 15q22.33-->q24.1 by in situ hybridization
Cytogenet. Cell Genet. 79 (1-2), 60-61 (1997)
Wang JY, Zhen DK, Bianchi DW, Androphy EJ and Chen JJ.


book

Interaction of papillomavirus E6 oncoproteins with a putative calcium-binding protein
Science 269 (5223), 529-531 (1995)
Chen JJ, Reid CE, Band V and Androphy EJ.


book

The endoplasmic reticulum calcium-binding protein of 55 kDa is a novel EF-hand protein retained in the endoplasmic reticulum by a carboxyl-terminal His-Asp-Glu-Leu motif
J. Biol. Chem. 269 (29), 19142-19150 (1994)
Weis K, Griffiths G and Lamond AI.


 
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