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RET cDNA ORF clone, Homo sapiens (human)

Gene Symbol RET
Entrez Gene ID 5979
Full Name ret proto-oncogene
Synonyms CDHF12, CDHR16, HSCR1, MEN2A, MEN2B, MTC1, PTC, RET-ELE1, RET51
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

10

10q11.2

Summary This gene, a member of the cadherin superfamily, encodes one of the receptor tyrosine kinases, which are cell-surface molecules that transduce signals for cell growth and differentiation. This gene plays a crucial role in neural crest development, and it can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Mutations in this gene are associated with the disorders multiple endocrine neoplasia, type IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease, and medullary thyroid carcinoma. Two transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their biological validity has not been confirmed. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

164761

Disorder Html: Multiple endocrine neoplasia IIA, 171400 (3); Medullary thyroid

mRNA and Protein(s)

mRNA Protein Name
XM_011540027 XP_011538329 proto-oncogene tyrosine-protein kinase receptor Ret isoform X1
NM_020975 NP_066124 proto-oncogene tyrosine-protein kinase receptor Ret isoform a precursor
NM_020630 NP_065681 proto-oncogene tyrosine-protein kinase receptor Ret isoform c precursor



Homo sapiens (human) RET NP_066124.1
Pan troglodytes (chimpanzee) RET XP_003312612.1
Macaca mulatta (Rhesus monkey) RET XP_002805672.1
Canis lupus familiaris (dog) RET NP_001184028.1
Bos taurus (cattle) RET NP_001178412.1
Mus musculus (house mouse) Ret NP_033076.2
Rattus norvegicus (Norway rat) Ret NP_036775.2
Gallus gallus (chicken) RET NP_990521.1
Danio rerio (zebrafish) ret NP_858048.2
Xenopus (Silurana) tropicalis (western clawed frog) ret XP_002933941.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RET gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RET cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu60238 XM_011540027 PREDICTED: Homo sapiens ret proto-oncogene (RET), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu27214
NM_020975 Homo sapiens ret proto-oncogene (RET), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$399.50-$559.30
$799.00
OHu27273
NM_020630 Homo sapiens ret proto-oncogene (RET), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu60238
Clone ID Related Accession (Same CDS sequence) XM_011540027
Accession Version XM_011540027.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3345bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product proto-oncogene tyrosine-protein kinase receptor Ret isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_030059.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
ATGGCGAAGG CGACGTCCGG TGCCGCGGGG CTGCGTCTGC TGTTGCTGCT GCTGCTGCCG 
CTGCTAGGCA AAGTGGCATT GGGCCTCTAC TTCTCGAGGG ATGCTTACTG GGAGAAGCTG
TATGTGGACC AGGCAGCCGG CACGCCCTTG CTGTACGTCC ATGCCCTGCG GGACGCCCCT
GAGGAGGTGC CCAGCTTCCG CCTGGGCCAG CATCTCTACG GCACGTACCG CACACGGCTG
CATGAGAACA ACTGGATCTG CATCCAGGAG GACACCGGCC TCCTCTACCT TAACCGGAGC
CTGGACCATA GCTCCTGGGA GAAGCTCAGT GTCCGCAACC GCGGCTTTCC CCTGCTCACC
GTCTACCTCA AGGTCTTCCT GTCACCCACA TCCCTTCGTG AGGGCGAGTG CCAGTGGCCA
GGCTGTGCCC GCGTATACTT CTCCTTCTTC AACACCTCCT TTCCAGCCTG CAGCTCCCTC
AAGCCCCGGG AGCTCTGCTT CCCAGAGACA AGGCCCTCCT TCCGCATTCG GGAGAACCGA
CCCCCAGGCA CCTTCCACCA GTTCCGCCTG CTGCCTGTGC AGTTCTTGTG CCCCAACATC
AGCGTGGCCT ACAGGCTCCT GGAGGGTGAG GGTCTGCCCT TCCGCTGCGC CCCGGACAGC
CTGGAGGTGA GCACGCGCTG GGCCCTGGAC CGCGAGCAGC GGGAGAAGTA CGAGCTGGTG
GCCGTGTGCA CCGTGCACGC CGGCGCGCGC GAGGAGGTGG TGATGGTGCC CTTCCCGGTG
ACCGTGTACG ACGAGGACGA CTCGGCGCCC ACCTTCCCCG CGGGCGTCGA CACCGCCAGC
GCCGTGGTGG AGTTCAAGCG GAAGGAGGAC ACCGTGGTGG CCACGCTGCG TGTCTTCGAT
GCAGACGTGG TACCTGCATC AGGGGAGCTG GTGAGGCGGT ACACAAGCAC GCTGCTCCCC
GGGGACACCT GGGCCCAGCA GACCTTCCGG GTGGAACACT GGCCCAACGA GACCTCGGTC
CAGGCCAACG GCAGCTTCGT GCGGGCGACC GTACATGACT ATAGGCTGGT TCTCAACCGG
AACCTCTCCA TCTCGGAGAA CCGCACCATG CAGCTGGCGG TGCTGGTCAA TGACTCAGAC
TTCCAGGGCC CAGGAGCGGG CGTCCTCTTG CTCCACTTCA ACGTGTCGGT GCTGCCGGTC
AGCCTGCACC TGCCCAGTAC CTACTCCCTC TCCGTGAGCA GGAGGGCTCG CCGATTTGCC
CAGATCGGGA AAGTCTGTGT GGAAAACTGC CAGGCATTCA GTGGCATCAA CGTCCAGTAC
AAGCTGCATT CCTCTGGTGC CAACTGCAGC ACGCTAGGGG TGGTCACCTC AGCCGAGGAC
ACCTCGGGGA TCCTGTTTGT GAATGACACC AAGGCCCTGC GGCGGCCCAA GTGTGCCGAA
CTTCACTACA TGGTGGTGGC CACCGACCAG CAGACCTCTA GGCAGGCCCA GGCCCAGCTG
CTTGTAACAG TGGAGGGGTC ATATGTGGCC GAGGAGGCGG GCTGCCCCCT GTCCTGTGCA
GTCAGCAAGA GACGGCTGGA GTGTGAGGAG TGTGGCGGCC TGGGCTCCCC AACAGGCAGG
TGTGAGTGGA GGCAAGGAGA TGGCAAAGGG ATCACCAGGA ACTTCTCCAC CTGCTCTCCC
AGCACCAAGA CCTGCCCCGA CGGCCACTGC GATGTTGTGG AGACCCAAGA CATCAACATT
TGCCCTCAGG ACTGCCTCCG GGGCAGCATT GTTGGGGGAC ACGAGCCTGG GGAGCCCCGG
GGGATTAAAG CTGGCTATGG CACCTGCAAC TGCTTCCCTG AGGAGGAGAA GTGCTTCTGC
GAGCCCGAAG ACATCCAGGA TCCACTGTGC GACGAGCTGT GCCGCACGGT GATCGCAGCC
GCTGTCCTCT TCTCCTTCAT CGTCTCGGTG CTGCTGTCTG CCTTCTGCAT CCACTGCTAC
CACAAGTTTG CCCACAAGCC ACCCATCTCC TCAGCTGAGA TGACCTTCCG GAGGCCCGCC
CAGGCCTTCC CGGTCAGCTA CTCCTCTTCC GGTGCCCGCC GGCCCTCGCT GGACTCCATG
GAGAACCAGG TCTCCGTGGA TGCCTTCAAG ATCCTGGAGG ATCCAAAGTG GGAATTCCCT
CGGAAGAACT TGGTTCTTGG AAAAACTCTA GGAGAAGGCG AATTTGGAAA AGTGGTCAAG
GCAACGGCCT TCCATCTGAA AGGCAGAGCA GGGTACACCA CGGTGGCCGT GAAGATGCTG
AAAGAGAACG CCTCCCCGAG TGAGCTGCGA GACCTGCTGT CAGAGTTCAA CGTCCTGAAG
CAGGTCAACC ACCCACATGT CATCAAATTG TATGGGGCCT GCAGCCAGGA TGGCCCGCTC
CTCCTCATCG TGGAGTACGC CAAATACGGC TCCCTGCGGG GCTTCCTCCG CGAGAGCCGC
AAAGTGGGGC CTGGCTACCT GGGCAGTGGA GGCAGCCGCA ACTCCAGCTC CCTGGACCAC
CCGGATGAGC GGGCCCTCAC CATGGGCGAC CTCATCTCAT TTGCCTGGCA GATCTCACAG
GGGATGCAGT ATCTGGCCGA GATGAAGCTC GTTCATCGGG ACTTGGCAGC CAGAAACATC
CTGGTAGCTG AGGGGCGGAA GATGAAGATT TCGGATTTCG GCTTGTCCCG AGATGTTTAT
GAAGAGGATT CCTACGTGAA GAGGAGCCAG GGTCGGATTC CAGTTAAATG GATGGCAATT
GAATCCCTTT TTGATCATAT CTACACCACG CAAAGTGATG TATGGTCTTT TGGTGTCCTG
CTGTGGGAGA TCGTGACCCT AGGGGGAAAC CCCTATCCTG GGATTCCTCC TGAGCGGCTC
TTCAACCTTC TGAAGACCGG CCACCGGATG GAGAGGCCAG ACAACTGCAG CGAGGAGATG
TACCGCCTGA TGCTGCAATG CTGGAAGCAG GAGCCGGACA AAAGGCCGGT GTTTGCGGAC
ATCAGCAAAG ACCTGGAGAA GATGATGGTT AAGAGGAGAG ACTACTTGGA CCTTGCGGCG
TCCACTCCAT CTGACTCCCT GATTTATGAC GACGGCCTCT CAGAGGAGGA GACACCGCTG
GTGGACTGTA ATAATGCCCC CCTCCCTCGA GCCCTCCCTT CCACATGGAT TGAAAACAAA
CTCTATGGCA TGTCAGACCC GAACTGGCCT GGAGAGAGTC CTGTACCACT CACGAGAGCT
GATGGCACTA ACACTGGGTT TCCAAGATAT CCAAATGATA GTGTATATGC TAACTGGATG
CTTTCACCCT CAGCGGCAAA ATTAATGGAC ACGTTTGATA GTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011538329.1
CDS233..3577
Misc Feature(1)749..1033(+)
Misc Feature(2)764..1033(+)
Misc Feature(3)2399..3268(+)
Misc Feature(4)2402..3247(+)
Misc Feature(5)2420..3103(+)
Misc Feature(6)2420..2908(+)
Misc Feature(7)2852..3103(+)
Misc Feature(8)2903..2980(+)
Translation

Target ORF information:

RefSeq Version XM_011540027
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens ret proto-oncogene (RET), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011540027

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
ATGGCGAAGG CGACGTCCGG TGCCGCGGGG CTGCGTCTGC TGTTGCTGCT GCTGCTGCCG 
CTGCTAGGCA AAGTGGCATT GGGCCTCTAC TTCTCGAGGG ATGCTTACTG GGAGAAGCTG
TATGTGGACC AGGCAGCCGG CACGCCCTTG CTGTACGTCC ATGCCCTGCG GGACGCCCCT
GAGGAGGTGC CCAGCTTCCG CCTGGGCCAG CATCTCTACG GCACGTACCG CACACGGCTG
CATGAGAACA ACTGGATCTG CATCCAGGAG GACACCGGCC TCCTCTACCT TAACCGGAGC
CTGGACCATA GCTCCTGGGA GAAGCTCAGT GTCCGCAACC GCGGCTTTCC CCTGCTCACC
GTCTACCTCA AGGTCTTCCT GTCACCCACA TCCCTTCGTG AGGGCGAGTG CCAGTGGCCA
GGCTGTGCCC GCGTATACTT CTCCTTCTTC AACACCTCCT TTCCAGCCTG CAGCTCCCTC
AAGCCCCGGG AGCTCTGCTT CCCAGAGACA AGGCCCTCCT TCCGCATTCG GGAGAACCGA
CCCCCAGGCA CCTTCCACCA GTTCCGCCTG CTGCCTGTGC AGTTCTTGTG CCCCAACATC
AGCGTGGCCT ACAGGCTCCT GGAGGGTGAG GGTCTGCCCT TCCGCTGCGC CCCGGACAGC
CTGGAGGTGA GCACGCGCTG GGCCCTGGAC CGCGAGCAGC GGGAGAAGTA CGAGCTGGTG
GCCGTGTGCA CCGTGCACGC CGGCGCGCGC GAGGAGGTGG TGATGGTGCC CTTCCCGGTG
ACCGTGTACG ACGAGGACGA CTCGGCGCCC ACCTTCCCCG CGGGCGTCGA CACCGCCAGC
GCCGTGGTGG AGTTCAAGCG GAAGGAGGAC ACCGTGGTGG CCACGCTGCG TGTCTTCGAT
GCAGACGTGG TACCTGCATC AGGGGAGCTG GTGAGGCGGT ACACAAGCAC GCTGCTCCCC
GGGGACACCT GGGCCCAGCA GACCTTCCGG GTGGAACACT GGCCCAACGA GACCTCGGTC
CAGGCCAACG GCAGCTTCGT GCGGGCGACC GTACATGACT ATAGGCTGGT TCTCAACCGG
AACCTCTCCA TCTCGGAGAA CCGCACCATG CAGCTGGCGG TGCTGGTCAA TGACTCAGAC
TTCCAGGGCC CAGGAGCGGG CGTCCTCTTG CTCCACTTCA ACGTGTCGGT GCTGCCGGTC
AGCCTGCACC TGCCCAGTAC CTACTCCCTC TCCGTGAGCA GGAGGGCTCG CCGATTTGCC
CAGATCGGGA AAGTCTGTGT GGAAAACTGC CAGGCATTCA GTGGCATCAA CGTCCAGTAC
AAGCTGCATT CCTCTGGTGC CAACTGCAGC ACGCTAGGGG TGGTCACCTC AGCCGAGGAC
ACCTCGGGGA TCCTGTTTGT GAATGACACC AAGGCCCTGC GGCGGCCCAA GTGTGCCGAA
CTTCACTACA TGGTGGTGGC CACCGACCAG CAGACCTCTA GGCAGGCCCA GGCCCAGCTG
CTTGTAACAG TGGAGGGGTC ATATGTGGCC GAGGAGGCGG GCTGCCCCCT GTCCTGTGCA
GTCAGCAAGA GACGGCTGGA GTGTGAGGAG TGTGGCGGCC TGGGCTCCCC AACAGGCAGG
TGTGAGTGGA GGCAAGGAGA TGGCAAAGGG ATCACCAGGA ACTTCTCCAC CTGCTCTCCC
AGCACCAAGA CCTGCCCCGA CGGCCACTGC GATGTTGTGG AGACCCAAGA CATCAACATT
TGCCCTCAGG ACTGCCTCCG GGGCAGCATT GTTGGGGGAC ACGAGCCTGG GGAGCCCCGG
GGGATTAAAG CTGGCTATGG CACCTGCAAC TGCTTCCCTG AGGAGGAGAA GTGCTTCTGC
GAGCCCGAAG ACATCCAGGA TCCACTGTGC GACGAGCTGT GCCGCACGGT GATCGCAGCC
GCTGTCCTCT TCTCCTTCAT CGTCTCGGTG CTGCTGTCTG CCTTCTGCAT CCACTGCTAC
CACAAGTTTG CCCACAAGCC ACCCATCTCC TCAGCTGAGA TGACCTTCCG GAGGCCCGCC
CAGGCCTTCC CGGTCAGCTA CTCCTCTTCC GGTGCCCGCC GGCCCTCGCT GGACTCCATG
GAGAACCAGG TCTCCGTGGA TGCCTTCAAG ATCCTGGAGG ATCCAAAGTG GGAATTCCCT
CGGAAGAACT TGGTTCTTGG AAAAACTCTA GGAGAAGGCG AATTTGGAAA AGTGGTCAAG
GCAACGGCCT TCCATCTGAA AGGCAGAGCA GGGTACACCA CGGTGGCCGT GAAGATGCTG
AAAGAGAACG CCTCCCCGAG TGAGCTGCGA GACCTGCTGT CAGAGTTCAA CGTCCTGAAG
CAGGTCAACC ACCCACATGT CATCAAATTG TATGGGGCCT GCAGCCAGGA TGGCCCGCTC
CTCCTCATCG TGGAGTACGC CAAATACGGC TCCCTGCGGG GCTTCCTCCG CGAGAGCCGC
AAAGTGGGGC CTGGCTACCT GGGCAGTGGA GGCAGCCGCA ACTCCAGCTC CCTGGACCAC
CCGGATGAGC GGGCCCTCAC CATGGGCGAC CTCATCTCAT TTGCCTGGCA GATCTCACAG
GGGATGCAGT ATCTGGCCGA GATGAAGCTC GTTCATCGGG ACTTGGCAGC CAGAAACATC
CTGGTAGCTG AGGGGCGGAA GATGAAGATT TCGGATTTCG GCTTGTCCCG AGATGTTTAT
GAAGAGGATT CCTACGTGAA GAGGAGCCAG GGTCGGATTC CAGTTAAATG GATGGCAATT
GAATCCCTTT TTGATCATAT CTACACCACG CAAAGTGATG TATGGTCTTT TGGTGTCCTG
CTGTGGGAGA TCGTGACCCT AGGGGGAAAC CCCTATCCTG GGATTCCTCC TGAGCGGCTC
TTCAACCTTC TGAAGACCGG CCACCGGATG GAGAGGCCAG ACAACTGCAG CGAGGAGATG
TACCGCCTGA TGCTGCAATG CTGGAAGCAG GAGCCGGACA AAAGGCCGGT GTTTGCGGAC
ATCAGCAAAG ACCTGGAGAA GATGATGGTT AAGAGGAGAG ACTACTTGGA CCTTGCGGCG
TCCACTCCAT CTGACTCCCT GATTTATGAC GACGGCCTCT CAGAGGAGGA GACACCGCTG
GTGGACTGTA ATAATGCCCC CCTCCCTCGA GCCCTCCCTT CCACATGGAT TGAAAACAAA
CTCTATGGCA TGTCAGACCC GAACTGGCCT GGAGAGAGTC CTGTACCACT CACGAGAGCT
GATGGCACTA ACACTGGGTT TCCAAGATAT CCAAATGATA GTGTATATGC TAACTGGATG
CTTTCACCCT CAGCGGCAAA ATTAATGGAC ACGTTTGATA GTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27214
Clone ID Related Accession (Same CDS sequence) NM_020975
Accession Version NM_020975.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3345bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 21-MAY-2015
Organism Homo sapiens (human)
Product proto-oncogene tyrosine-protein kinase receptor Ret isoform a precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA100452.1, BC003072.2, BC004257.1, X12949.1, AC010864.11 and BM661773.1. This sequence is a reference standard in the RefSeqGene project. On Feb 27, 2007 this sequence version replaced gi:50593519. Transcript Variant: This variant (2) represents the longer transcript and encodes the longer isoform (a). This isoform is also known as Ret51. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
ATGGCGAAGG CGACGTCCGG TGCCGCGGGG CTGCGTCTGC TGTTGCTGCT GCTGCTGCCG 
CTGCTAGGCA AAGTGGCATT GGGCCTCTAC TTCTCGAGGG ATGCTTACTG GGAGAAGCTG
TATGTGGACC AGGCGGCCGG CACGCCCTTG CTGTACGTCC ATGCCCTGCG GGACGCCCCT
GAGGAGGTGC CCAGCTTCCG CCTGGGCCAG CATCTCTACG GCACGTACCG CACACGGCTG
CATGAGAACA ACTGGATCTG CATCCAGGAG GACACCGGCC TCCTCTACCT TAACCGGAGC
CTGGACCATA GCTCCTGGGA GAAGCTCAGT GTCCGCAACC GCGGCTTTCC CCTGCTCACC
GTCTACCTCA AGGTCTTCCT GTCACCCACA TCCCTTCGTG AGGGCGAGTG CCAGTGGCCA
GGCTGTGCCC GCGTATACTT CTCCTTCTTC AACACCTCCT TTCCAGCCTG CAGCTCCCTC
AAGCCCCGGG AGCTCTGCTT CCCAGAGACA AGGCCCTCCT TCCGCATTCG GGAGAACCGA
CCCCCAGGCA CCTTCCACCA GTTCCGCCTG CTGCCTGTGC AGTTCTTGTG CCCCAACATC
AGCGTGGCCT ACAGGCTCCT GGAGGGTGAG GGTCTGCCCT TCCGCTGCGC CCCGGACAGC
CTGGAGGTGA GCACGCGCTG GGCCCTGGAC CGCGAGCAGC GGGAGAAGTA CGAGCTGGTG
GCCGTGTGCA CCGTGCACGC CGGCGCGCGC GAGGAGGTGG TGATGGTGCC CTTCCCGGTG
ACCGTGTACG ACGAGGACGA CTCGGCGCCC ACCTTCCCCG CGGGCGTCGA CACCGCCAGC
GCCGTGGTGG AGTTCAAGCG GAAGGAGGAC ACCGTGGTGG CCACGCTGCG TGTCTTCGAT
GCAGACGTGG TACCTGCATC AGGGGAGCTG GTGAGGCGGT ACACAAGCAC GCTGCTCCCC
GGGGACACCT GGGCCCAGCA GACCTTCCGG GTGGAACACT GGCCCAACGA GACCTCGGTC
CAGGCCAACG GCAGCTTCGT GCGGGCGACC GTACATGACT ATAGGCTGGT TCTCAACCGG
AACCTCTCCA TCTCGGAGAA CCGCACCATG CAGCTGGCGG TGCTGGTCAA TGACTCAGAC
TTCCAGGGCC CAGGAGCGGG CGTCCTCTTG CTCCACTTCA ACGTGTCGGT GCTGCCGGTC
AGCCTGCACC TGCCCAGTAC CTACTCCCTC TCCGTGAGCA GGAGGGCTCG CCGATTTGCC
CAGATCGGGA AAGTCTGTGT GGAAAACTGC CAGGCATTCA GTGGCATCAA CGTCCAGTAC
AAGCTGCATT CCTCTGGTGC CAACTGCAGC ACGCTAGGGG TGGTCACCTC AGCCGAGGAC
ACCTCGGGGA TCCTGTTTGT GAATGACACC AAGGCCCTGC GGCGGCCCAA GTGTGCCGAA
CTTCACTACA TGGTGGTGGC CACCGACCAG CAGACCTCTA GGCAGGCCCA GGCCCAGCTG
CTTGTAACAG TGGAGGGGTC ATATGTGGCC GAGGAGGCGG GCTGCCCCCT GTCCTGTGCA
GTCAGCAAGA GACGGCTGGA GTGTGAGGAG TGTGGCGGCC TGGGCTCCCC AACAGGCAGG
TGTGAGTGGA GGCAAGGAGA TGGCAAAGGG ATCACCAGGA ACTTCTCCAC CTGCTCTCCC
AGCACCAAGA CCTGCCCCGA CGGCCACTGC GATGTTGTGG AGACCCAAGA CATCAACATT
TGCCCTCAGG ACTGCCTCCG GGGCAGCATT GTTGGGGGAC ACGAGCCTGG GGAGCCCCGG
GGGATTAAAG CTGGCTATGG CACCTGCAAC TGCTTCCCTG AGGAGGAGAA GTGCTTCTGC
GAGCCCGAAG ACATCCAGGA TCCACTGTGC GACGAGCTGT GCCGCACGGT GATCGCAGCC
GCTGTCCTCT TCTCCTTCAT CGTCTCGGTG CTGCTGTCTG CCTTCTGCAT CCACTGCTAC
CACAAGTTTG CCCACAAGCC ACCCATCTCC TCAGCTGAGA TGACCTTCCG GAGGCCCGCC
CAGGCCTTCC CGGTCAGCTA CTCCTCTTCC GGTGCCCGCC GGCCCTCGCT GGACTCCATG
GAGAACCAGG TCTCCGTGGA TGCCTTCAAG ATCCTGGAGG ATCCAAAGTG GGAATTCCCT
CGGAAGAACT TGGTTCTTGG AAAAACTCTA GGAGAAGGCG AATTTGGAAA AGTGGTCAAG
GCAACGGCCT TCCATCTGAA AGGCAGAGCA GGGTACACCA CGGTGGCCGT GAAGATGCTG
AAAGAGAACG CCTCCCCGAG TGAGCTTCGA GACCTGCTGT CAGAGTTCAA CGTCCTGAAG
CAGGTCAACC ACCCACATGT CATCAAATTG TATGGGGCCT GCAGCCAGGA TGGCCCGCTC
CTCCTCATCG TGGAGTACGC CAAATACGGC TCCCTGCGGG GCTTCCTCCG CGAGAGCCGC
AAAGTGGGGC CTGGCTACCT GGGCAGTGGA GGCAGCCGCA ACTCCAGCTC CCTGGACCAC
CCGGATGAGC GGGCCCTCAC CATGGGCGAC CTCATCTCAT TTGCCTGGCA GATCTCACAG
GGGATGCAGT ATCTGGCCGA GATGAAGCTC GTTCATCGGG ACTTGGCAGC CAGAAACATC
CTGGTAGCTG AGGGGCGGAA GATGAAGATT TCGGATTTCG GCTTGTCCCG AGATGTTTAT
GAAGAGGATT CCTACGTGAA GAGGAGCCAG GGTCGGATTC CAGTTAAATG GATGGCAATT
GAATCCCTTT TTGATCATAT CTACACCACG CAAAGTGATG TATGGTCTTT TGGTGTCCTG
CTGTGGGAGA TCGTGACCCT AGGGGGAAAC CCCTATCCTG GGATTCCTCC TGAGCGGCTC
TTCAACCTTC TGAAGACCGG CCACCGGATG GAGAGGCCAG ACAACTGCAG CGAGGAGATG
TACCGCCTGA TGCTGCAATG CTGGAAGCAG GAGCCGGACA AAAGGCCGGT GTTTGCGGAC
ATCAGCAAAG ACCTGGAGAA GATGATGGTT AAGAGGAGAG ACTACTTGGA CCTTGCGGCG
TCCACTCCAT CTGACTCCCT GATTTATGAC GACGGCCTCT CAGAGGAGGA GACACCGCTG
GTGGACTGTA ATAATGCCCC CCTCCCTCGA GCCCTCCCTT CCACATGGAT TGAAAACAAA
CTCTATGGCA TGTCAGACCC GAACTGGCCT GGAGAGAGTC CTGTACCACT CACGAGAGCT
GATGGCACTA ACACTGGGTT TCCAAGATAT CCAAATGATA GTGTATATGC TAACTGGATG
CTTTCACCCT CAGCGGCAAA ATTAATGGAC ACGTTTGATA GTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_066124.1
CDS191..3535
Misc Feature(1)149..151(+)
Misc Feature(2)707..991(+)
Misc Feature(3)722..991(+)
Misc Feature(4)1949..1954(+)
Misc Feature(5)2096..2161(+)
Misc Feature(6)2249..2251(+)
Misc Feature(7)2276..2278(+)
Misc Feature(8)2309..2314(+)
Misc Feature(9)2324..2329(+)
Misc Feature(10)2357..3226(+)
Misc Feature(11)2360..3205(+)
Misc Feature(12)2378..3061(+)
Misc Feature(13)2378..2866(+)
Misc Feature(14)2603..2611(+)
Misc Feature(15)2606..2608(+)
Misc Feature(16)2606..2608(+)
Misc Feature(17)2615..2617(+)
Misc Feature(18)2615..2617(+)
Misc Feature(19)2666..2668(+)
Misc Feature(20)2810..3061(+)
Misc Feature(21)2861..2938(+)
Misc Feature(22)2888..2890(+)
Misc Feature(23)2888..2890(+)
Misc Feature(24)2903..2905(+)
Misc Feature(25)2903..2905(+)
Misc Feature(26)3131..3133(+)
Misc Feature(27)3131..3133(+)
Misc Feature(28)3233..3235(+)
Misc Feature(29)3233..3235(+)
Misc Feature(30)3239..3244(+)
Misc Feature(31)3275..3277(+)
Misc Feature(32)3374..3376(+)
Misc Feature(33)3374..3376(+)
Misc Feature(34)3458..3460(+)
Misc Feature(35)3458..3460(+)
Misc Feature(36)3476..3478(+)
Misc Feature(37)3476..3478(+)
Exon (1)1..263
Gene:RET
Gene Synonym:
Exon (2)264..527
Gene:RET
Gene Synonym:
Exon (3)528..815
Gene:RET
Gene Synonym:
Exon (4)816..1057
Gene:RET
Gene Synonym:
Exon (5)1058..1253
Gene:RET
Gene Synonym:
Exon (6)1254..1453
Gene:RET
Gene Synonym:
Exon (7)1454..1712
Gene:RET
Gene Synonym:
Exon (8)1713..1838
Gene:RET
Gene Synonym:
Exon (9)1839..1949
Gene:RET
Gene Synonym:
Exon (10)1950..2069
Gene:RET
Gene Synonym:
Exon (11)2070..2326
Gene:RET
Gene Synonym:
Exon (12)2327..2474
Gene:RET
Gene Synonym:
Exon (13)2475..2582
Gene:RET
Gene Synonym:
Exon (14)2583..2797
Gene:RET
Gene Synonym:
Exon (15)2798..2920
Gene:RET
Gene Synonym:
Exon (16)2921..2991
Gene:RET
Gene Synonym:
Exon (17)2992..3129
Gene:RET
Gene Synonym:
Exon (18)3130..3229
Gene:RET
Gene Synonym:
Exon (19)3230..3377
Gene:RET
Gene Synonym:
Exon (20)3378..5617
Gene:RET
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_020975
Organism Homo sapiens (human)
Definition Homo sapiens ret proto-oncogene (RET), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_020975

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
ATGGCGAAGG CGACGTCCGG TGCCGCGGGG CTGCGTCTGC TGTTGCTGCT GCTGCTGCCG 
CTGCTAGGCA AAGTGGCATT GGGCCTCTAC TTCTCGAGGG ATGCTTACTG GGAGAAGCTG
TATGTGGACC AGGCGGCCGG CACGCCCTTG CTGTACGTCC ATGCCCTGCG GGACGCCCCT
GAGGAGGTGC CCAGCTTCCG CCTGGGCCAG CATCTCTACG GCACGTACCG CACACGGCTG
CATGAGAACA ACTGGATCTG CATCCAGGAG GACACCGGCC TCCTCTACCT TAACCGGAGC
CTGGACCATA GCTCCTGGGA GAAGCTCAGT GTCCGCAACC GCGGCTTTCC CCTGCTCACC
GTCTACCTCA AGGTCTTCCT GTCACCCACA TCCCTTCGTG AGGGCGAGTG CCAGTGGCCA
GGCTGTGCCC GCGTATACTT CTCCTTCTTC AACACCTCCT TTCCAGCCTG CAGCTCCCTC
AAGCCCCGGG AGCTCTGCTT CCCAGAGACA AGGCCCTCCT TCCGCATTCG GGAGAACCGA
CCCCCAGGCA CCTTCCACCA GTTCCGCCTG CTGCCTGTGC AGTTCTTGTG CCCCAACATC
AGCGTGGCCT ACAGGCTCCT GGAGGGTGAG GGTCTGCCCT TCCGCTGCGC CCCGGACAGC
CTGGAGGTGA GCACGCGCTG GGCCCTGGAC CGCGAGCAGC GGGAGAAGTA CGAGCTGGTG
GCCGTGTGCA CCGTGCACGC CGGCGCGCGC GAGGAGGTGG TGATGGTGCC CTTCCCGGTG
ACCGTGTACG ACGAGGACGA CTCGGCGCCC ACCTTCCCCG CGGGCGTCGA CACCGCCAGC
GCCGTGGTGG AGTTCAAGCG GAAGGAGGAC ACCGTGGTGG CCACGCTGCG TGTCTTCGAT
GCAGACGTGG TACCTGCATC AGGGGAGCTG GTGAGGCGGT ACACAAGCAC GCTGCTCCCC
GGGGACACCT GGGCCCAGCA GACCTTCCGG GTGGAACACT GGCCCAACGA GACCTCGGTC
CAGGCCAACG GCAGCTTCGT GCGGGCGACC GTACATGACT ATAGGCTGGT TCTCAACCGG
AACCTCTCCA TCTCGGAGAA CCGCACCATG CAGCTGGCGG TGCTGGTCAA TGACTCAGAC
TTCCAGGGCC CAGGAGCGGG CGTCCTCTTG CTCCACTTCA ACGTGTCGGT GCTGCCGGTC
AGCCTGCACC TGCCCAGTAC CTACTCCCTC TCCGTGAGCA GGAGGGCTCG CCGATTTGCC
CAGATCGGGA AAGTCTGTGT GGAAAACTGC CAGGCATTCA GTGGCATCAA CGTCCAGTAC
AAGCTGCATT CCTCTGGTGC CAACTGCAGC ACGCTAGGGG TGGTCACCTC AGCCGAGGAC
ACCTCGGGGA TCCTGTTTGT GAATGACACC AAGGCCCTGC GGCGGCCCAA GTGTGCCGAA
CTTCACTACA TGGTGGTGGC CACCGACCAG CAGACCTCTA GGCAGGCCCA GGCCCAGCTG
CTTGTAACAG TGGAGGGGTC ATATGTGGCC GAGGAGGCGG GCTGCCCCCT GTCCTGTGCA
GTCAGCAAGA GACGGCTGGA GTGTGAGGAG TGTGGCGGCC TGGGCTCCCC AACAGGCAGG
TGTGAGTGGA GGCAAGGAGA TGGCAAAGGG ATCACCAGGA ACTTCTCCAC CTGCTCTCCC
AGCACCAAGA CCTGCCCCGA CGGCCACTGC GATGTTGTGG AGACCCAAGA CATCAACATT
TGCCCTCAGG ACTGCCTCCG GGGCAGCATT GTTGGGGGAC ACGAGCCTGG GGAGCCCCGG
GGGATTAAAG CTGGCTATGG CACCTGCAAC TGCTTCCCTG AGGAGGAGAA GTGCTTCTGC
GAGCCCGAAG ACATCCAGGA TCCACTGTGC GACGAGCTGT GCCGCACGGT GATCGCAGCC
GCTGTCCTCT TCTCCTTCAT CGTCTCGGTG CTGCTGTCTG CCTTCTGCAT CCACTGCTAC
CACAAGTTTG CCCACAAGCC ACCCATCTCC TCAGCTGAGA TGACCTTCCG GAGGCCCGCC
CAGGCCTTCC CGGTCAGCTA CTCCTCTTCC GGTGCCCGCC GGCCCTCGCT GGACTCCATG
GAGAACCAGG TCTCCGTGGA TGCCTTCAAG ATCCTGGAGG ATCCAAAGTG GGAATTCCCT
CGGAAGAACT TGGTTCTTGG AAAAACTCTA GGAGAAGGCG AATTTGGAAA AGTGGTCAAG
GCAACGGCCT TCCATCTGAA AGGCAGAGCA GGGTACACCA CGGTGGCCGT GAAGATGCTG
AAAGAGAACG CCTCCCCGAG TGAGCTTCGA GACCTGCTGT CAGAGTTCAA CGTCCTGAAG
CAGGTCAACC ACCCACATGT CATCAAATTG TATGGGGCCT GCAGCCAGGA TGGCCCGCTC
CTCCTCATCG TGGAGTACGC CAAATACGGC TCCCTGCGGG GCTTCCTCCG CGAGAGCCGC
AAAGTGGGGC CTGGCTACCT GGGCAGTGGA GGCAGCCGCA ACTCCAGCTC CCTGGACCAC
CCGGATGAGC GGGCCCTCAC CATGGGCGAC CTCATCTCAT TTGCCTGGCA GATCTCACAG
GGGATGCAGT ATCTGGCCGA GATGAAGCTC GTTCATCGGG ACTTGGCAGC CAGAAACATC
CTGGTAGCTG AGGGGCGGAA GATGAAGATT TCGGATTTCG GCTTGTCCCG AGATGTTTAT
GAAGAGGATT CCTACGTGAA GAGGAGCCAG GGTCGGATTC CAGTTAAATG GATGGCAATT
GAATCCCTTT TTGATCATAT CTACACCACG CAAAGTGATG TATGGTCTTT TGGTGTCCTG
CTGTGGGAGA TCGTGACCCT AGGGGGAAAC CCCTATCCTG GGATTCCTCC TGAGCGGCTC
TTCAACCTTC TGAAGACCGG CCACCGGATG GAGAGGCCAG ACAACTGCAG CGAGGAGATG
TACCGCCTGA TGCTGCAATG CTGGAAGCAG GAGCCGGACA AAAGGCCGGT GTTTGCGGAC
ATCAGCAAAG ACCTGGAGAA GATGATGGTT AAGAGGAGAG ACTACTTGGA CCTTGCGGCG
TCCACTCCAT CTGACTCCCT GATTTATGAC GACGGCCTCT CAGAGGAGGA GACACCGCTG
GTGGACTGTA ATAATGCCCC CCTCCCTCGA GCCCTCCCTT CCACATGGAT TGAAAACAAA
CTCTATGGCA TGTCAGACCC GAACTGGCCT GGAGAGAGTC CTGTACCACT CACGAGAGCT
GATGGCACTA ACACTGGGTT TCCAAGATAT CCAAATGATA GTGTATATGC TAACTGGATG
CTTTCACCCT CAGCGGCAAA ATTAATGGAC ACGTTTGATA GTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27273
Clone ID Related Accession (Same CDS sequence) NM_020630
Accession Version NM_020630.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3219bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 21-MAY-2015
Organism Homo sapiens (human)
Product proto-oncogene tyrosine-protein kinase receptor Ret isoform c precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA100452.1, BC003072.2, BC004257.1, X12949.1, DA911581.1, BM703293.1, BE261914.1 and AI472270.1. This sequence is a reference standard in the RefSeqGene project. On Feb 27, 2007 this sequence version replaced gi:50593520. Transcript Variant: This variant (4) differs in the 3' UTR and coding region compared to variant 2. The resulting isoform (c) is shorter and has a distinct C-terminus compared to isoform a. This isoform is also known as Ret9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC004257.1, AK291807.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGGCGAAGG CGACGTCCGG TGCCGCGGGG CTGCGTCTGC TGTTGCTGCT GCTGCTGCCG 
CTGCTAGGCA AAGTGGCATT GGGCCTCTAC TTCTCGAGGG ATGCTTACTG GGAGAAGCTG
TATGTGGACC AGGCGGCCGG CACGCCCTTG CTGTACGTCC ATGCCCTGCG GGACGCCCCT
GAGGAGGTGC CCAGCTTCCG CCTGGGCCAG CATCTCTACG GCACGTACCG CACACGGCTG
CATGAGAACA ACTGGATCTG CATCCAGGAG GACACCGGCC TCCTCTACCT TAACCGGAGC
CTGGACCATA GCTCCTGGGA GAAGCTCAGT GTCCGCAACC GCGGCTTTCC CCTGCTCACC
GTCTACCTCA AGGTCTTCCT GTCACCCACA TCCCTTCGTG AGGGCGAGTG CCAGTGGCCA
GGCTGTGCCC GCGTATACTT CTCCTTCTTC AACACCTCCT TTCCAGCCTG CAGCTCCCTC
AAGCCCCGGG AGCTCTGCTT CCCAGAGACA AGGCCCTCCT TCCGCATTCG GGAGAACCGA
CCCCCAGGCA CCTTCCACCA GTTCCGCCTG CTGCCTGTGC AGTTCTTGTG CCCCAACATC
AGCGTGGCCT ACAGGCTCCT GGAGGGTGAG GGTCTGCCCT TCCGCTGCGC CCCGGACAGC
CTGGAGGTGA GCACGCGCTG GGCCCTGGAC CGCGAGCAGC GGGAGAAGTA CGAGCTGGTG
GCCGTGTGCA CCGTGCACGC CGGCGCGCGC GAGGAGGTGG TGATGGTGCC CTTCCCGGTG
ACCGTGTACG ACGAGGACGA CTCGGCGCCC ACCTTCCCCG CGGGCGTCGA CACCGCCAGC
GCCGTGGTGG AGTTCAAGCG GAAGGAGGAC ACCGTGGTGG CCACGCTGCG TGTCTTCGAT
GCAGACGTGG TACCTGCATC AGGGGAGCTG GTGAGGCGGT ACACAAGCAC GCTGCTCCCC
GGGGACACCT GGGCCCAGCA GACCTTCCGG GTGGAACACT GGCCCAACGA GACCTCGGTC
CAGGCCAACG GCAGCTTCGT GCGGGCGACC GTACATGACT ATAGGCTGGT TCTCAACCGG
AACCTCTCCA TCTCGGAGAA CCGCACCATG CAGCTGGCGG TGCTGGTCAA TGACTCAGAC
TTCCAGGGCC CAGGAGCGGG CGTCCTCTTG CTCCACTTCA ACGTGTCGGT GCTGCCGGTC
AGCCTGCACC TGCCCAGTAC CTACTCCCTC TCCGTGAGCA GGAGGGCTCG CCGATTTGCC
CAGATCGGGA AAGTCTGTGT GGAAAACTGC CAGGCATTCA GTGGCATCAA CGTCCAGTAC
AAGCTGCATT CCTCTGGTGC CAACTGCAGC ACGCTAGGGG TGGTCACCTC AGCCGAGGAC
ACCTCGGGGA TCCTGTTTGT GAATGACACC AAGGCCCTGC GGCGGCCCAA GTGTGCCGAA
CTTCACTACA TGGTGGTGGC CACCGACCAG CAGACCTCTA GGCAGGCCCA GGCCCAGCTG
CTTGTAACAG TGGAGGGGTC ATATGTGGCC GAGGAGGCGG GCTGCCCCCT GTCCTGTGCA
GTCAGCAAGA GACGGCTGGA GTGTGAGGAG TGTGGCGGCC TGGGCTCCCC AACAGGCAGG
TGTGAGTGGA GGCAAGGAGA TGGCAAAGGG ATCACCAGGA ACTTCTCCAC CTGCTCTCCC
AGCACCAAGA CCTGCCCCGA CGGCCACTGC GATGTTGTGG AGACCCAAGA CATCAACATT
TGCCCTCAGG ACTGCCTCCG GGGCAGCATT GTTGGGGGAC ACGAGCCTGG GGAGCCCCGG
GGGATTAAAG CTGGCTATGG CACCTGCAAC TGCTTCCCTG AGGAGGAGAA GTGCTTCTGC
GAGCCCGAAG ACATCCAGGA TCCACTGTGC GACGAGCTGT GCCGCACGGT GATCGCAGCC
GCTGTCCTCT TCTCCTTCAT CGTCTCGGTG CTGCTGTCTG CCTTCTGCAT CCACTGCTAC
CACAAGTTTG CCCACAAGCC ACCCATCTCC TCAGCTGAGA TGACCTTCCG GAGGCCCGCC
CAGGCCTTCC CGGTCAGCTA CTCCTCTTCC GGTGCCCGCC GGCCCTCGCT GGACTCCATG
GAGAACCAGG TCTCCGTGGA TGCCTTCAAG ATCCTGGAGG ATCCAAAGTG GGAATTCCCT
CGGAAGAACT TGGTTCTTGG AAAAACTCTA GGAGAAGGCG AATTTGGAAA AGTGGTCAAG
GCAACGGCCT TCCATCTGAA AGGCAGAGCA GGGTACACCA CGGTGGCCGT GAAGATGCTG
AAAGAGAACG CCTCCCCGAG TGAGCTTCGA GACCTGCTGT CAGAGTTCAA CGTCCTGAAG
CAGGTCAACC ACCCACATGT CATCAAATTG TATGGGGCCT GCAGCCAGGA TGGCCCGCTC
CTCCTCATCG TGGAGTACGC CAAATACGGC TCCCTGCGGG GCTTCCTCCG CGAGAGCCGC
AAAGTGGGGC CTGGCTACCT GGGCAGTGGA GGCAGCCGCA ACTCCAGCTC CCTGGACCAC
CCGGATGAGC GGGCCCTCAC CATGGGCGAC CTCATCTCAT TTGCCTGGCA GATCTCACAG
GGGATGCAGT ATCTGGCCGA GATGAAGCTC GTTCATCGGG ACTTGGCAGC CAGAAACATC
CTGGTAGCTG AGGGGCGGAA GATGAAGATT TCGGATTTCG GCTTGTCCCG AGATGTTTAT
GAAGAGGATT CCTACGTGAA GAGGAGCCAG GGTCGGATTC CAGTTAAATG GATGGCAATT
GAATCCCTTT TTGATCATAT CTACACCACG CAAAGTGATG TATGGTCTTT TGGTGTCCTG
CTGTGGGAGA TCGTGACCCT AGGGGGAAAC CCCTATCCTG GGATTCCTCC TGAGCGGCTC
TTCAACCTTC TGAAGACCGG CCACCGGATG GAGAGGCCAG ACAACTGCAG CGAGGAGATG
TACCGCCTGA TGCTGCAATG CTGGAAGCAG GAGCCGGACA AAAGGCCGGT GTTTGCGGAC
ATCAGCAAAG ACCTGGAGAA GATGATGGTT AAGAGGAGAG ACTACTTGGA CCTTGCGGCG
TCCACTCCAT CTGACTCCCT GATTTATGAC GACGGCCTCT CAGAGGAGGA GACACCGCTG
GTGGACTGTA ATAATGCCCC CCTCCCTCGA GCCCTCCCTT CCACATGGAT TGAAAACAAA
CTCTATGGTA GAATTTCCCA TGCATTTACT AGATTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_065681.1
CDS191..3409
Misc Feature(1)149..151(+)
Misc Feature(2)707..991(+)
Misc Feature(3)722..991(+)
Misc Feature(4)1949..1954(+)
Misc Feature(5)2096..2161(+)
Misc Feature(6)2249..2251(+)
Misc Feature(7)2276..2278(+)
Misc Feature(8)2309..2314(+)
Misc Feature(9)2324..2329(+)
Misc Feature(10)2357..3226(+)
Misc Feature(11)2360..3205(+)
Misc Feature(12)2378..3061(+)
Misc Feature(13)2378..2866(+)
Misc Feature(14)2603..2611(+)
Misc Feature(15)2606..2608(+)
Misc Feature(16)2606..2608(+)
Misc Feature(17)2615..2617(+)
Misc Feature(18)2615..2617(+)
Misc Feature(19)2666..2668(+)
Misc Feature(20)2810..3061(+)
Misc Feature(21)2861..2938(+)
Misc Feature(22)2888..2890(+)
Misc Feature(23)2888..2890(+)
Misc Feature(24)2903..2905(+)
Misc Feature(25)2903..2905(+)
Misc Feature(26)3131..3133(+)
Misc Feature(27)3131..3133(+)
Misc Feature(28)3233..3235(+)
Misc Feature(29)3233..3235(+)
Misc Feature(30)3239..3244(+)
Misc Feature(31)3275..3277(+)
Misc Feature(32)3374..3376(+)
Misc Feature(33)3374..3376(+)
Exon (1)1..263
Gene:RET
Gene Synonym:
Exon (2)264..527
Gene:RET
Gene Synonym:
Exon (3)528..815
Gene:RET
Gene Synonym:
Exon (4)816..1057
Gene:RET
Gene Synonym:
Exon (5)1058..1253
Gene:RET
Gene Synonym:
Exon (6)1254..1453
Gene:RET
Gene Synonym:
Exon (7)1454..1712
Gene:RET
Gene Synonym:
Exon (8)1713..1838
Gene:RET
Gene Synonym:
Exon (9)1839..1949
Gene:RET
Gene Synonym:
Exon (10)1950..2069
Gene:RET
Gene Synonym:
Exon (11)2070..2326
Gene:RET
Gene Synonym:
Exon (12)2327..2474
Gene:RET
Gene Synonym:
Exon (13)2475..2582
Gene:RET
Gene Synonym:
Exon (14)2583..2797
Gene:RET
Gene Synonym:
Exon (15)2798..2920
Gene:RET
Gene Synonym:
Exon (16)2921..2991
Gene:RET
Gene Synonym:
Exon (17)2992..3129
Gene:RET
Gene Synonym:
Exon (18)3130..3229
Gene:RET
Gene Synonym:
Exon (19)3230..4159
Gene:RET
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_020630
Organism Homo sapiens (human)
Definition Homo sapiens ret proto-oncogene (RET), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_020630

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGGCGAAGG CGACGTCCGG TGCCGCGGGG CTGCGTCTGC TGTTGCTGCT GCTGCTGCCG 
CTGCTAGGCA AAGTGGCATT GGGCCTCTAC TTCTCGAGGG ATGCTTACTG GGAGAAGCTG
TATGTGGACC AGGCGGCCGG CACGCCCTTG CTGTACGTCC ATGCCCTGCG GGACGCCCCT
GAGGAGGTGC CCAGCTTCCG CCTGGGCCAG CATCTCTACG GCACGTACCG CACACGGCTG
CATGAGAACA ACTGGATCTG CATCCAGGAG GACACCGGCC TCCTCTACCT TAACCGGAGC
CTGGACCATA GCTCCTGGGA GAAGCTCAGT GTCCGCAACC GCGGCTTTCC CCTGCTCACC
GTCTACCTCA AGGTCTTCCT GTCACCCACA TCCCTTCGTG AGGGCGAGTG CCAGTGGCCA
GGCTGTGCCC GCGTATACTT CTCCTTCTTC AACACCTCCT TTCCAGCCTG CAGCTCCCTC
AAGCCCCGGG AGCTCTGCTT CCCAGAGACA AGGCCCTCCT TCCGCATTCG GGAGAACCGA
CCCCCAGGCA CCTTCCACCA GTTCCGCCTG CTGCCTGTGC AGTTCTTGTG CCCCAACATC
AGCGTGGCCT ACAGGCTCCT GGAGGGTGAG GGTCTGCCCT TCCGCTGCGC CCCGGACAGC
CTGGAGGTGA GCACGCGCTG GGCCCTGGAC CGCGAGCAGC GGGAGAAGTA CGAGCTGGTG
GCCGTGTGCA CCGTGCACGC CGGCGCGCGC GAGGAGGTGG TGATGGTGCC CTTCCCGGTG
ACCGTGTACG ACGAGGACGA CTCGGCGCCC ACCTTCCCCG CGGGCGTCGA CACCGCCAGC
GCCGTGGTGG AGTTCAAGCG GAAGGAGGAC ACCGTGGTGG CCACGCTGCG TGTCTTCGAT
GCAGACGTGG TACCTGCATC AGGGGAGCTG GTGAGGCGGT ACACAAGCAC GCTGCTCCCC
GGGGACACCT GGGCCCAGCA GACCTTCCGG GTGGAACACT GGCCCAACGA GACCTCGGTC
CAGGCCAACG GCAGCTTCGT GCGGGCGACC GTACATGACT ATAGGCTGGT TCTCAACCGG
AACCTCTCCA TCTCGGAGAA CCGCACCATG CAGCTGGCGG TGCTGGTCAA TGACTCAGAC
TTCCAGGGCC CAGGAGCGGG CGTCCTCTTG CTCCACTTCA ACGTGTCGGT GCTGCCGGTC
AGCCTGCACC TGCCCAGTAC CTACTCCCTC TCCGTGAGCA GGAGGGCTCG CCGATTTGCC
CAGATCGGGA AAGTCTGTGT GGAAAACTGC CAGGCATTCA GTGGCATCAA CGTCCAGTAC
AAGCTGCATT CCTCTGGTGC CAACTGCAGC ACGCTAGGGG TGGTCACCTC AGCCGAGGAC
ACCTCGGGGA TCCTGTTTGT GAATGACACC AAGGCCCTGC GGCGGCCCAA GTGTGCCGAA
CTTCACTACA TGGTGGTGGC CACCGACCAG CAGACCTCTA GGCAGGCCCA GGCCCAGCTG
CTTGTAACAG TGGAGGGGTC ATATGTGGCC GAGGAGGCGG GCTGCCCCCT GTCCTGTGCA
GTCAGCAAGA GACGGCTGGA GTGTGAGGAG TGTGGCGGCC TGGGCTCCCC AACAGGCAGG
TGTGAGTGGA GGCAAGGAGA TGGCAAAGGG ATCACCAGGA ACTTCTCCAC CTGCTCTCCC
AGCACCAAGA CCTGCCCCGA CGGCCACTGC GATGTTGTGG AGACCCAAGA CATCAACATT
TGCCCTCAGG ACTGCCTCCG GGGCAGCATT GTTGGGGGAC ACGAGCCTGG GGAGCCCCGG
GGGATTAAAG CTGGCTATGG CACCTGCAAC TGCTTCCCTG AGGAGGAGAA GTGCTTCTGC
GAGCCCGAAG ACATCCAGGA TCCACTGTGC GACGAGCTGT GCCGCACGGT GATCGCAGCC
GCTGTCCTCT TCTCCTTCAT CGTCTCGGTG CTGCTGTCTG CCTTCTGCAT CCACTGCTAC
CACAAGTTTG CCCACAAGCC ACCCATCTCC TCAGCTGAGA TGACCTTCCG GAGGCCCGCC
CAGGCCTTCC CGGTCAGCTA CTCCTCTTCC GGTGCCCGCC GGCCCTCGCT GGACTCCATG
GAGAACCAGG TCTCCGTGGA TGCCTTCAAG ATCCTGGAGG ATCCAAAGTG GGAATTCCCT
CGGAAGAACT TGGTTCTTGG AAAAACTCTA GGAGAAGGCG AATTTGGAAA AGTGGTCAAG
GCAACGGCCT TCCATCTGAA AGGCAGAGCA GGGTACACCA CGGTGGCCGT GAAGATGCTG
AAAGAGAACG CCTCCCCGAG TGAGCTTCGA GACCTGCTGT CAGAGTTCAA CGTCCTGAAG
CAGGTCAACC ACCCACATGT CATCAAATTG TATGGGGCCT GCAGCCAGGA TGGCCCGCTC
CTCCTCATCG TGGAGTACGC CAAATACGGC TCCCTGCGGG GCTTCCTCCG CGAGAGCCGC
AAAGTGGGGC CTGGCTACCT GGGCAGTGGA GGCAGCCGCA ACTCCAGCTC CCTGGACCAC
CCGGATGAGC GGGCCCTCAC CATGGGCGAC CTCATCTCAT TTGCCTGGCA GATCTCACAG
GGGATGCAGT ATCTGGCCGA GATGAAGCTC GTTCATCGGG ACTTGGCAGC CAGAAACATC
CTGGTAGCTG AGGGGCGGAA GATGAAGATT TCGGATTTCG GCTTGTCCCG AGATGTTTAT
GAAGAGGATT CCTACGTGAA GAGGAGCCAG GGTCGGATTC CAGTTAAATG GATGGCAATT
GAATCCCTTT TTGATCATAT CTACACCACG CAAAGTGATG TATGGTCTTT TGGTGTCCTG
CTGTGGGAGA TCGTGACCCT AGGGGGAAAC CCCTATCCTG GGATTCCTCC TGAGCGGCTC
TTCAACCTTC TGAAGACCGG CCACCGGATG GAGAGGCCAG ACAACTGCAG CGAGGAGATG
TACCGCCTGA TGCTGCAATG CTGGAAGCAG GAGCCGGACA AAAGGCCGGT GTTTGCGGAC
ATCAGCAAAG ACCTGGAGAA GATGATGGTT AAGAGGAGAG ACTACTTGGA CCTTGCGGCG
TCCACTCCAT CTGACTCCCT GATTTATGAC GACGGCCTCT CAGAGGAGGA GACACCGCTG
GTGGACTGTA ATAATGCCCC CCTCCCTCGA GCCCTCCCTT CCACATGGAT TGAAAACAAA
CTCTATGGTA GAATTTCCCA TGCATTTACT AGATTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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