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ALX4 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol ALX4
Entrez Gene ID 60529
Full Name ALX homeobox 4
Synonyms CRS5,FND2
General protein information
Preferred Names

ALX homeobox 4

Names

homeobox protein aristaless-like 4
aristaless-like homeobox 4
homeodomain transcription factor ALX4

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

11

11p11.2

Summary This gene encodes a paired-like homeodomain transcription factor expressed in the mesenchyme of developing bones, limbs, hair, teeth, and mammary tissue. Mutations in this gene cause parietal foramina 2 (PFM2); an autosomal dominant disease characterized by deficient ossification of the parietal bones. Mutations in this gene also cause a form of frontonasal dysplasia with alopecia and hypogonadism; suggesting a role for this gene in craniofacial development, mesenchymal-epithelial communication, and hair follicle development. Deletion of a segment of chromosome 11 containing this gene, del(11)(p11p12), causes Potocki-Shaffer syndrome (PSS); a syndrome characterized by craniofacial anomalies, mental retardation, multiple exostoses, and genital abnormalities in males. In mouse, this gene has been shown to use dual translation initiation sites located 16 codons apart. [provided by RefSeq, Oct 2009].
Disorder MIM:

605420

Disorder Html: Parietal foramina 2, 609597 (3); Frontonasal dysplasia 2, 613451 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_021926.3 NP_068745.2 homeobox protein aristaless-like 4



Mus musculus (house mouse) Alx4 NP_031468.1
Canis lupus familiaris (dog) ALX4 XP_850737.1
Macaca mulatta (rhesus monkey) ALX4 XP_001113643.1
Homo sapiens (human) ALX4 NP_068745.2
Rattus norvegicus (Norway rat) Alx4 NP_001100023.1
Gallus gallus (chicken) ALX4 NP_989493.1
Danio rerio (zebrafish) alx4a XP_001340966.1
Xenopus tropicalis (tropical clawed frog) alx4 XP_002937332.2
Bos taurus (cattle) ALX4 NP_001025475.1
Danio rerio (zebrafish) alx4b NP_001082826.1
Pan troglodytes (chimpanzee) ALX4 XP_521895.3


Related articles in PubMed

Solution NMR structures of homeodomains from human proteins ALX4, ZHX1, and CASP8AP2 contribute to the structural coverage of the Human Cancer Protein Interaction Network.
Xu X, Pulavarti SV, Eletsky A, Huang YJ, Acton TB, Xiao R, Everett JK, Montelione GT, Szyperski T
Journal of structural and functional genomics15(4)201-7(2014 Dec)

Mild nasal clefting may be predictive for ALX4 heterozygotes.
Altunoglu U, Satkın B, Uyguner ZO, Kayserili H
American journal of medical genetics. Part A164A(8)2054-8(2014 Aug)

Epigenetic silencing of Aristaless-like homeobox-4, a potential tumor suppressor gene associated with lung cancer.
Liu WB, Han F, Du XH, Jiang X, Li YH, Liu Y, Chen HQ, Ao L, Cui ZH, Cao J, Liu JY
International journal of cancer134(6)1311-22(2014 Mar)

Genome-wide association study identifies 8 novel loci associated with blood pressure responses to interventions in Han Chinese.
He J, Kelly TN, Zhao Q, Li H, Huang J, Wang L, Jaquish CE, Sung YJ, Shimmin LC, Lu F, Mu J, Hu D, Ji X, Shen C, Guo D, Ma J, Wang R, Shen J, Li S, Chen J, Mei H, Chen CS, Chen S, Chen J, Li J, Cao J, Lu X, Wu X, Rice TK, Gu CC, Schwander K, Hamm LL, Liu D, Rao DC, Hixson JE, Gu D
Circulation. Cardiovascular genetics6(6)598-607(2013 Dec)

Vertical transmission of a frontonasal phenotype caused by a novel ALX4 mutation.
Bertola DR, Rodrigues MG, Quaio CR, Kim CA, Passos-Bueno MR
American journal of medical genetics. Part A161A(3)600-4(2013 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

overexpression of ALX4 inhibited the proliferation, invasion, and epithelial-mesenchymal transition (EMT) in HCC cells. ALX4 had an inhibitory effect on the sonic hedgehog (Shh) signaling pathway.
Title: Overexpression of Aristaless-Like Homeobox-4 Inhibits Proliferation, Invasion, and EMT in Hepatocellular Carcinoma Cells.

mother of Case 2 also had a mutation in the ALX4 gene, but no enlarged parietal foramina
Title: [Mother and son with enlarged parietal foramina, persistent fetal vein, and ALX4 mutation].

Our results show that HOXB13/SLUG and ALX4/SLUG axes are novel pathways that promote EMT and invasion of ovarian cancer cells.
Title: HOXB13 and ALX4 induce SLUG expression for the promotion of EMT and cell invasion in ovarian cancer cells.

High-quality solution NMR structures of three homeodomains from human proteins ALX4, ZHX1 and CASP8AP2 were solved.
Title: Solution NMR structures of homeodomains from human proteins ALX4, ZHX1, and CASP8AP2 contribute to the structural coverage of the Human Cancer Protein Interaction Network.

We suggest that all ALX4 heterozygote carriers be examined in detail for possible changes in nasal configuration, to establish a detailed genotype-phenotype correlation, leading the way to more comprehensive genetic counseling.
Title: Mild nasal clefting may be predictive for ALX4 heterozygotes.

The following ALX4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALX4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu22706 NM_021926.3
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Homo sapiens ALX homeobox 4 (ALX4), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
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$319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu22706
Clone ID Related Accession (Same CDS sequence) NM_021926.3
Accession Version NM_021926.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1236bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-09-25
Organism Homo sapiens(Human)
Product homeobox protein aristaless-like 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF294629.1, AB058691.1 and AC103854.2. This sequence is a reference standard in the RefSeqGene project. On Oct 10, 2009 this sequence version replaced NM_021926.2. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF294629.1, AB058691.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGAATGCTG AGACTTGCGT CTCTTACTGC GAGTCGCCGG CCGCTGCCAT GGACGCCTAC 
TACAGCCCGG TGTCGCAGAG TCGGGAGGGC TCGTCGCCTT TTAGGGCATT TCCCGGAGGC
GACAAGTTCG GCACAACTTT CCTGTCGGCC GCCGCCAAAG CACAGGGATT CGGGGACGCC
AAGAGCCGGG CCCGTTACGG CGCTGGGCAG CAGGACCTGG CGACACCCCT GGAGAGTGGA
GCTGGGGCGC GGGGCTCCTT TAACAAGTTC CAGCCCCAGC CGTCGACCCC GCAGCCCCAG
CCGCCGCCGC AGCCGCAGCC GCAGCAGCAG CAGCCGCAGC CCCAGCCGCC CGCGCAACCG
CATCTTTACT TGCAGCGAGG CGCCTGCAAG ACGCCCCCGG ACGGCAGCCT CAAACTCCAG
GAAGGCAGCA GCGGCCACAG CGCGGCCTTG CAGGTTCCCT GCTACGCTAA AGAGAGCTCC
CTGGGTGAGC CAGAGTTACC CCCTGACTCT GACACTGTGG GGATGGACAG CAGCTACCTG
AGTGTCAAGG AGGCTGGGGT GAAGGGGCCC CAGGACCGGG CCAGCTCAGA CCTCCCCAGC
CCATTGGAGA AGGCCGACTC AGAGAGCAAC AAGGGCAAGA AGCGGCGGAA CCGGACCACC
TTCACCAGCT ACCAGCTGGA GGAGCTGGAG AAGGTCTTCC AGAAGACCCA CTACCCAGAC
GTGTATGCGC GGGAACAGCT GGCCATGAGG ACAGACCTCA CTGAGGCCCG CGTGCAGGTC
TGGTTCCAGA ACCGAAGGGC CAAGTGGAGG AAGCGGGAGC GTTTTGGGCA GATGCAGCAG
GTTCGAACCC ACTTCTCCAC TGCATATGAG CTGCCCCTCC TCACCCGAGC TGAGAACTAC
GCCCAGATTC AGAACCCGTC CTGGCTCGGC AACAACGGGG CTGCCTCACC AGTGCCAGCC
TGCGTGGTCC CCTGCGACCC GGTGCCTGCC TGCATGTCCC CTCATGCCCA CCCCCCTGGC
TCTGGGGCCA GCAGCGTCAC CGACTTCCTG AGTGTGTCTG GGGCTGGCAG TCACGTGGGC
CAGACGCACA TGGGCAGCCT GTTTGGAGCA GCCAGCCTCA GCCCAGGCCT CAATGGCTAC
GAGCTCAACG GCGAGCCGGA CCGCAAGACC TCGAGCATCG CGGCCCTCCG CATGAAGGCC
AAGGAGCACA GTGCGGCCAT TTCCTGGGCC ACATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_068745.2
CDS105..1340
Misc Feature(1)order(747..761,765..767,816..818,834..836,873..875,879..884,891..896,900..908,912..917)
Misc Feature(2)order(753..755,762..764,882..884,891..896,903..905)
Misc Feature(3)756..914
Misc Feature(4)1263..1316
Misc Feature(5)1275..1316
Exon (1)1..570
Exon (2)571..881
Exon (3)882..1010
Exon (4)1011..5466
Translation

Target ORF information:

RefSeq Version NM_021926.3
Organism Homo sapiens(Human)
Definition Homo sapiens ALX homeobox 4 (ALX4), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_021926.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGAATGCTG AGACTTGCGT CTCTTACTGC GAGTCGCCGG CCGCTGCCAT GGACGCCTAC 
TACAGCCCGG TGTCGCAGAG TCGGGAGGGC TCGTCGCCTT TTAGGGCATT TCCCGGAGGC
GACAAGTTCG GCACAACTTT CCTGTCGGCC GCCGCCAAAG CACAGGGATT CGGGGACGCC
AAGAGCCGGG CCCGTTACGG CGCTGGGCAG CAGGACCTGG CGACACCCCT GGAGAGTGGA
GCTGGGGCGC GGGGCTCCTT TAACAAGTTC CAGCCCCAGC CGTCGACCCC GCAGCCCCAG
CCGCCGCCGC AGCCGCAGCC GCAGCAGCAG CAGCCGCAGC CCCAGCCGCC CGCGCAACCG
CATCTTTACT TGCAGCGAGG CGCCTGCAAG ACGCCCCCGG ACGGCAGCCT CAAACTCCAG
GAAGGCAGCA GCGGCCACAG CGCGGCCTTG CAGGTTCCCT GCTACGCTAA AGAGAGCTCC
CTGGGTGAGC CAGAGTTACC CCCTGACTCT GACACTGTGG GGATGGACAG CAGCTACCTG
AGTGTCAAGG AGGCTGGGGT GAAGGGGCCC CAGGACCGGG CCAGCTCAGA CCTCCCCAGC
CCATTGGAGA AGGCCGACTC AGAGAGCAAC AAGGGCAAGA AGCGGCGGAA CCGGACCACC
TTCACCAGCT ACCAGCTGGA GGAGCTGGAG AAGGTCTTCC AGAAGACCCA CTACCCAGAC
GTGTATGCGC GGGAACAGCT GGCCATGAGG ACAGACCTCA CTGAGGCCCG CGTGCAGGTC
TGGTTCCAGA ACCGAAGGGC CAAGTGGAGG AAGCGGGAGC GTTTTGGGCA GATGCAGCAG
GTTCGAACCC ACTTCTCCAC TGCATATGAG CTGCCCCTCC TCACCCGAGC TGAGAACTAC
GCCCAGATTC AGAACCCGTC CTGGCTCGGC AACAACGGGG CTGCCTCACC AGTGCCAGCC
TGCGTGGTCC CCTGCGACCC GGTGCCTGCC TGCATGTCCC CTCATGCCCA CCCCCCTGGC
TCTGGGGCCA GCAGCGTCAC CGACTTCCTG AGTGTGTCTG GGGCTGGCAG TCACGTGGGC
CAGACGCACA TGGGCAGCCT GTTTGGAGCA GCCAGCCTCA GCCCAGGCCT CAATGGCTAC
GAGCTCAACG GCGAGCCGGA CCGCAAGACC TCGAGCATCG CGGCCCTCCG CATGAAGGCC
AAGGAGCACA GTGCGGCCAT TTCCTGGGCC ACATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Solution NMR structures of homeodomains from human proteins ALX4, ZHX1, and CASP8AP2 contribute to the structural coverage of the Human Cancer Protein Interaction Network.
Journal of structural and functional genomics15(4)201-7(2014 Dec)
Xu X,Pulavarti SV,Eletsky A,Huang YJ,Acton TB,Xiao R,Everett JK,Montelione GT,Szyperski T


book

Mild nasal clefting may be predictive for ALX4 heterozygotes.
American journal of medical genetics. Part A164A(8)2054-8(2014 Aug)
Altunoglu U,Satkın B,Uyguner ZO,Kayserili H


book

Epigenetic silencing of Aristaless-like homeobox-4, a potential tumor suppressor gene associated with lung cancer.
International journal of cancer134(6)1311-22(2014 Mar)
Liu WB,Han F,Du XH,Jiang X,Li YH,Liu Y,Chen HQ,Ao L,Cui ZH,Cao J,Liu JY


book

Genome-wide association study identifies 8 novel loci associated with blood pressure responses to interventions in Han Chinese.
Circulation. Cardiovascular genetics6(6)598-607(2013 Dec)
He J,Kelly TN,Zhao Q,Li H,Huang J,Wang L,Jaquish CE,Sung YJ,Shimmin LC,Lu F,Mu J,Hu D,Ji X,Shen C,Guo D,Ma J,Wang R,Shen J,Li S,Chen J,Mei H,Chen CS,Chen S,Chen J,Li J,Cao J,Lu X,Wu X,Rice TK,Gu CC,Schwander K,Hamm LL,Liu D,Rao DC,Hixson JE,Gu D


book

Vertical transmission of a frontonasal phenotype caused by a novel ALX4 mutation.
American journal of medical genetics. Part A161A(3)600-4(2013 Mar)
Bertola DR,Rodrigues MG,Quaio CR,Kim CA,Passos-Bueno MR


 
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