DHX35 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following DHX35 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DHX35 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu53075 XM_006723848.3
Latest version!
Homo sapiens DEAH-box helicase 35 (DHX35), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OHu71096 XM_017028004.1
Latest version!
Homo sapiens DEAH-box helicase 35 (DHX35), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu71097 XM_011528974.2
Latest version!
Homo sapiens DEAH-box helicase 35 (DHX35), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu05033 NM_001190809.2
Latest version!
Homo sapiens DEAH-box helicase 35 (DHX35), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu32249 NM_021931.4
Latest version!
Homo sapiens DEAH-box helicase 35 (DHX35), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $265.30
$379.00
OHu71095 XM_011528972.2
Latest version!
Homo sapiens DEAH-box helicase 35 (DHX35), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
View Old Accession Versions >>
OHu32249 NM_021931.3 Homo sapiens DEAH-box helicase 35 (DHX35), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $265.30
$379.00
OHu71097 XM_011528974.1 Homo sapiens DEAH-box helicase 35 (DHX35), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu05033 NM_001190809.1 Homo sapiens DEAH-box helicase 35 (DHX35), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu71095 XM_011528972.1 Homo sapiens DEAH-box helicase 35 (DHX35), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu53075
    Clone ID Related Accession (Same CDS sequence) XM_006723848.3
    Accession Version XM_006723848.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 930bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product probable ATP-dependent RNA helicase DHX35 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006723848.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGTTATCGC CTTTATACAG TTCTGATTTC CTATCCTCAG AGGAAGCCTT TGACAAGTTG 
    CCTCAGTCTA CGGTTCCTGA GATGCAGCGT AGTAATTTGG CACCTGTCAT CCTGCAGCTG
    AAAGCACTAG GAATTGACAA TGTCCTCAGG TTCCACTTCA TGTCGCCCCC TCCAGCACAG
    TCGATGGTTC AAGCCTTGGA GTTACTGTAT GCTCTGGGAG GTCTGGACAA AGACTGTCGC
    CTAACTGAAC CGCTTGGCAT GAGAATTGCA GAGTTTCCTT TGAATCCCAT GTTTGCCAAA
    ATGCTGCTTG AATCAGGAAA CTTCGGCTGT TCTCAGGAAA TTCTAAGCAT CGCTGCCATG
    ATGCAGATCC AGAATATCTT TGTGGTCCCC CCAAACCAGA AGTCTCACGC AATTCGAGTG
    CACCGTAAAT TTGCTGTGGA GGAGGGCGAC CACCTCACTA TGCTCAATAT ATATGAAGCA
    TTTATCAAAC ACAATAAGGA CTCTAAATGG TGTCAGGAAC ATTTCCTGAA TTACAAGGGT
    CTTGTCAGAG CTGCGACTGT AAGAGAACAA TTGAAAAAGC TTCTTGTCAA GTTTCAAGTG
    CCCAGGAAGT CTAGTGAAGG TGACCCGGAT CTGGTTCTGA GGTGCATTGT CTCCGGCTTC
    TTCGCCAATG CAGCGAGGTT TCATTCTACT GGAGCTTATA GGACCATCCG TGATGACCAT
    GAGCTGCACA TACACCCTGC GTCAGTCCTC TATGCAGAGA AGCCGCCTCG CTGGGTCATC
    TATAACGAAG TTATACAGAC CTCCAAGTAC TACATGAGAG ATGTGACTGC CATTGAATCG
    GCCTGGCTGT TGGAGCTGGC TCCACACTTT TATCAACAAG GAACGCACCT GTCTCTGAAA
    GCCAAAAGGG CCAAGGTCCA GGACCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006723911.1
    CDS118..1047
    Translation

    Target ORF information:

    RefSeq Version XM_006723848.3
    Organism Homo sapiens(human)
    Definition Homo sapiens DEAH-box helicase 35 (DHX35), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006723848.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGTTATCGC CTTTATACAG TTCTGATTTC CTATCCTCAG AGGAAGCCTT TGACAAGTTG 
    CCTCAGTCTA CGGTTCCTGA GATGCAGCGT AGTAATTTGG CACCTGTCAT CCTGCAGCTG
    AAAGCACTAG GAATTGACAA TGTCCTCAGG TTCCACTTCA TGTCGCCCCC TCCAGCACAG
    TCGATGGTTC AAGCCTTGGA GTTACTGTAT GCTCTGGGAG GTCTGGACAA AGACTGTCGC
    CTAACTGAAC CGCTTGGCAT GAGAATTGCA GAGTTTCCTT TGAATCCCAT GTTTGCCAAA
    ATGCTGCTTG AATCAGGAAA CTTCGGCTGT TCTCAGGAAA TTCTAAGCAT CGCTGCCATG
    ATGCAGATCC AGAATATCTT TGTGGTCCCC CCAAACCAGA AGTCTCACGC AATTCGAGTG
    CACCGTAAAT TTGCTGTGGA GGAGGGCGAC CACCTCACTA TGCTCAATAT ATATGAAGCA
    TTTATCAAAC ACAATAAGGA CTCTAAATGG TGTCAGGAAC ATTTCCTGAA TTACAAGGGT
    CTTGTCAGAG CTGCGACTGT AAGAGAACAA TTGAAAAAGC TTCTTGTCAA GTTTCAAGTG
    CCCAGGAAGT CTAGTGAAGG TGACCCGGAT CTGGTTCTGA GGTGCATTGT CTCCGGCTTC
    TTCGCCAATG CAGCGAGGTT TCATTCTACT GGAGCTTATA GGACCATCCG TGATGACCAT
    GAGCTGCACA TACACCCTGC GTCAGTCCTC TATGCAGAGA AGCCGCCTCG CTGGGTCATC
    TATAACGAAG TTATACAGAC CTCCAAGTAC TACATGAGAG ATGTGACTGC CATTGAATCG
    GCCTGGCTGT TGGAGCTGGC TCCACACTTT TATCAACAAG GAACGCACCT GTCTCTGAAA
    GCCAAAAGGG CCAAGGTCCA GGACCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71096
    Clone ID Related Accession (Same CDS sequence) XM_017028004.1
    Accession Version XM_017028004.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1647bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product probable ATP-dependent RNA helicase DHX35 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGCTGGTCA GGGAAATGAT GGTTGATCCG TTGTTAACAA AATATAGTGT CATCATGCTG 
    GATGAAGCCC ACGAGAGGAC CTTGTACACT GACATTGCCA TTGGCTTGCT AAAAAAGATT
    CAGAAAAAGC GAGGGGATCT TCGATTGATT GTAGCTTCAG CCACTCTGGA TGCAGACAAA
    TTCCGGGATT TCTTTAATCA AAATGAAACC AGTGATCCAG CAAGGGATAC ATGTGTGATC
    CTTACAGTGG AAGGGAGAAC ATTTCCGGTG GATATCTTTT ATCTACAAAG TCCTGTTCCA
    GATTATATCA AATCAACTGT CGAAACTGTG GTGAAAATTC ACCAGACAGA GGGAGACGGA
    GACGTTTTAG CATTTCTTAC TGGCCAGGAA GAGGTAGAAA CTGTTGTGTC GATGCTCATC
    GAGCAGGCTC GAGCACTAGC TCGCACTGGG ATGAAGAGAC ACCTCCGAGT TCTCCCCATG
    TATGCAGGAC TGCCTTCCTT TGAGCAAATG AAAGTGTTTG AAAGGGTGTC ACGCAGTGTC
    AGAAAGGTGA TAGTGGCCAC CAATGTGGCA GAAACCTCTA TCACAATCAG CGGCATTGTG
    TATGTGATCG ACTGTGGCTT TGTGAAACTC CGAGCCTACA ATCCCAGGAC AGCTATTGAA
    TGCTTGGTGG TGGTGCCAGT CTCCCAGGCA TCAGCTAATC AGCGAGCAGG ACGTGGTGGT
    CGTAGTCGCT CGGGAAAATG TTATCGCCTT TATACAGAGG AAGCCTTTGA CAAGTTGCCT
    CAGTCTACGG TTCCTGAGAT GCAGCGTAGT AATTTGGCAC CTGTCATCCT GCAGCTGAAA
    GCACTAGGAA TTGACAATGT CCTCAGGTTC CACTTCATGT CGCCCCCTCC AGCACAGTCG
    ATGGTTCAAG CCTTGGAGTT ACTGTATGCT CTGGGAGGTC TGGACAAAGA CTGTCGCCTA
    ACTGAACCGC TTGGCATGAG AATTGCAGAG TTTCCTTTGA ATCCCATGTT TGCCAAAATG
    CTGCTTGAAT CAGGAAACTT CGGCTGTTCT CAGGAAATTC TAAGCATCGC TGCCATGATG
    CAGATCCAGA ATATCTTTGT GGTCCCCCCA AACCAGAAGT CTCACGCAAT TCGAGTGCAC
    CGTAAATTTG CTGTGGAGGA GGGCGACCAC CTCACTATGC TCAATATATA TGAAGCATTT
    ATCAAACACA ATAAGGACTC TAAATGGTGT CAGGAACATT TCCTGAATTA CAAGGGTCTT
    GTCAGAGCTG CGACTGTAAG AGAACAATTG AAAAAGCTTC TTGTCAAGTT TCAAGTGCCC
    AGGAAGTCTA GTGAAGGTGA CCCGGATCTG GTTCTGAGGT GCATTGTCTC CGGCTTCTTC
    GCCAATGCAG CGAGGTTTCA TTCTACTGGA GCTTATAGGA CCATCCGTGA TGACCATGAG
    CTGCACATAC ACCCTGCGTC AGTCCTCTAT GCAGAGAAGC CGCCTCGCTG GGTCATCTAT
    AACGAAGTTA TACAGACCTC CAAGTACTAC ATGAGAGATG TGACTGCCAT TGAATCGGCC
    TGGCTGTTGG AGCTGGCTCC ACACTTTTAT CAACAAGGAA CGCACCTGTC TCTGAAAGCC
    AAAAGGGCCA AGGTCCAGGA CCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016883493.1
    CDS199..1845
    Translation

    Target ORF information:

    RefSeq Version XM_017028004.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DEAH-box helicase 35 (DHX35), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017028004.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGCTGGTCA GGGAAATGAT GGTTGATCCG TTGTTAACAA AATATAGTGT CATCATGCTG 
    GATGAAGCCC ACGAGAGGAC CTTGTACACT GACATTGCCA TTGGCTTGCT AAAAAAGATT
    CAGAAAAAGC GAGGGGATCT TCGATTGATT GTAGCTTCAG CCACTCTGGA TGCAGACAAA
    TTCCGGGATT TCTTTAATCA AAATGAAACC AGTGATCCAG CAAGGGATAC ATGTGTGATC
    CTTACAGTGG AAGGGAGAAC ATTTCCGGTG GATATCTTTT ATCTACAAAG TCCTGTTCCA
    GATTATATCA AATCAACTGT CGAAACTGTG GTGAAAATTC ACCAGACAGA GGGAGACGGA
    GACGTTTTAG CATTTCTTAC TGGCCAGGAA GAGGTAGAAA CTGTTGTGTC GATGCTCATC
    GAGCAGGCTC GAGCACTAGC TCGCACTGGG ATGAAGAGAC ACCTCCGAGT TCTCCCCATG
    TATGCAGGAC TGCCTTCCTT TGAGCAAATG AAAGTGTTTG AAAGGGTGTC ACGCAGTGTC
    AGAAAGGTGA TAGTGGCCAC CAATGTGGCA GAAACCTCTA TCACAATCAG CGGCATTGTG
    TATGTGATCG ACTGTGGCTT TGTGAAACTC CGAGCCTACA ATCCCAGGAC AGCTATTGAA
    TGCTTGGTGG TGGTGCCAGT CTCCCAGGCA TCAGCTAATC AGCGAGCAGG ACGTGGTGGT
    CGTAGTCGCT CGGGAAAATG TTATCGCCTT TATACAGAGG AAGCCTTTGA CAAGTTGCCT
    CAGTCTACGG TTCCTGAGAT GCAGCGTAGT AATTTGGCAC CTGTCATCCT GCAGCTGAAA
    GCACTAGGAA TTGACAATGT CCTCAGGTTC CACTTCATGT CGCCCCCTCC AGCACAGTCG
    ATGGTTCAAG CCTTGGAGTT ACTGTATGCT CTGGGAGGTC TGGACAAAGA CTGTCGCCTA
    ACTGAACCGC TTGGCATGAG AATTGCAGAG TTTCCTTTGA ATCCCATGTT TGCCAAAATG
    CTGCTTGAAT CAGGAAACTT CGGCTGTTCT CAGGAAATTC TAAGCATCGC TGCCATGATG
    CAGATCCAGA ATATCTTTGT GGTCCCCCCA AACCAGAAGT CTCACGCAAT TCGAGTGCAC
    CGTAAATTTG CTGTGGAGGA GGGCGACCAC CTCACTATGC TCAATATATA TGAAGCATTT
    ATCAAACACA ATAAGGACTC TAAATGGTGT CAGGAACATT TCCTGAATTA CAAGGGTCTT
    GTCAGAGCTG CGACTGTAAG AGAACAATTG AAAAAGCTTC TTGTCAAGTT TCAAGTGCCC
    AGGAAGTCTA GTGAAGGTGA CCCGGATCTG GTTCTGAGGT GCATTGTCTC CGGCTTCTTC
    GCCAATGCAG CGAGGTTTCA TTCTACTGGA GCTTATAGGA CCATCCGTGA TGACCATGAG
    CTGCACATAC ACCCTGCGTC AGTCCTCTAT GCAGAGAAGC CGCCTCGCTG GGTCATCTAT
    AACGAAGTTA TACAGACCTC CAAGTACTAC ATGAGAGATG TGACTGCCAT TGAATCGGCC
    TGGCTGTTGG AGCTGGCTCC ACACTTTTAT CAACAAGGAA CGCACCTGTC TCTGAAAGCC
    AAAAGGGCCA AGGTCCAGGA CCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71097
    Clone ID Related Accession (Same CDS sequence) XM_011528974.2 , XM_011528974.1
    Accession Version XM_011528974.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product probable ATP-dependent RNA helicase DHX35 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011528974.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGCTGCGC CCGTGGGACC GGTGAAGTTC TGGCGACCCG GTACAGAGGG GCCAGGTGTA 
    AGCATCTCTG AAGAGAGACA AAGTCTGGCT GAAAACTCTG GGACAACGGT TGTTTACAAC
    CCTTATGCTG CCCTTTCCAT AGAGCAGCAG AGGCAGAAGC TGCCGGTATT CAAGCTTAGG
    AATCATATTT TATACTTGAT AGAAAATTAT CAGACAGTGG TGATTGTTGG TGAAACAGGA
    TGTGGGAAGA GCACACAGAT TCCTCAGTAC CTTGCAGAAG CCGGCTGGAC AGCTGAAGGA
    AGAGTGGTAG GAGTGACCCA GCCTCGAAGA GTGGCTGCTG TTACAGTTGC AGGGAGAGTA
    GCTGAAGAAA GGGGTGCAGT GCTGGGCCAC GAGGTGGGCT ACTGCATCCG CTTTGATGAC
    TGCACCGACC AGCTGGCCAC GAGAATTAAG TTTCTTACTG ATGGAATGCT GGTCAGGGAA
    ATGATGGTTG ATCCGTTGTT AACAAAATAT AGTGTCATCA TGCTGGATGA AGCCCACGAG
    AGGACCTTGT ACACTGACAT TGCCATTGGC TTGCTAAAAA AGATTCAGAA AAAGCGAGGG
    GATCTTCGAT TGATTGTAGC TTCAGCCACT CTGGATGCAG ACAAATTCCG GGATTTCTTT
    AATCAAAATG AAACCAGTGA TCCAGCAAGG GATACATGTG TGATCCTTAC AGTGGAAGGG
    AGAACATTTC CGGTGGATAT CTTTTATCTA CAAAGTCCTG TTCCAGATTA TATCAAATCA
    ACTGTCGAAA CTGTGGTGAA AATTCACCAG ACAGAGGGAG ACGGAGACGT TTTAGCATTT
    CTTACTGGCC AGGAAGAGGT AGAAACTGTT GTGTCGATGC TCATCGAGCA GGCTCGAGCA
    CTAGCTCGCA CTGGGATGAA GAGACACCTC CGAGTTCTCC CCATGTATGC AGGACTGCCT
    TCCTTTGAGC AAATGAAAGT GTTTGAAAGG GTGTCACGCA GTGTCAGAAA GGTGATAGTG
    GCCACCAATG TGGCAGAAAC CTCTATCACA ATCAGCGGCA TTGTGTATGT GATCGACTGT
    GGCTTTGTGA AACTCCGAGC CTACAATCCC AGGACAGCTA TTGAATGCTT GGTGGTGGTG
    CCAGTCTCCC AGGCATCAGC TAATCAGCGA GCAGGACGTG GTGGTCGTAG TCGCTCGGGA
    AAATGTTATC GCCTTTATAC AGAGGAAGCC TTTGACAAGT TGCCTCAGTC TACGGTTCCT
    GAGATGCAGC GTAGTAATTT GGCACCTGTC ATCCTGCAGC TGAAAGCACT AGGAATTGAC
    AATGTCCTCA GGTTCCACTT CATGTCGGTC ATGTGTTGTG TGTCTGCTTT GCAACCAGGC
    CCTGTGCAGG ACGTAGGGGA AAACAGCCAG ACAAGCCCCC TCCAGCACAG TCGATGGTTC
    AAGCCTTGGA GTTACTGTAT GCTCTGGGAG GTCTGGACAA AGACTGTCGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011527276.1
    CDS25..1518
    Translation

    Target ORF information:

    RefSeq Version XM_011528974.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DEAH-box helicase 35 (DHX35), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011528974.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGCTGCGC CCGTGGGACC GGTGAAGTTC TGGCGACCCG GTACAGAGGG GCCAGGTGTA 
    AGCATCTCTG AAGAGAGACA AAGTCTGGCT GAAAACTCTG GGACAACGGT TGTTTACAAC
    CCTTATGCTG CCCTTTCCAT AGAGCAGCAG AGGCAGAAGC TGCCGGTATT CAAGCTTAGG
    AATCATATTT TATACTTGAT AGAAAATTAT CAGACAGTGG TGATTGTTGG TGAAACAGGA
    TGTGGGAAGA GCACACAGAT TCCTCAGTAC CTTGCAGAAG CCGGCTGGAC AGCTGAAGGA
    AGAGTGGTAG GAGTGACCCA GCCTCGAAGA GTGGCTGCTG TTACAGTTGC AGGGAGAGTA
    GCTGAAGAAA GGGGTGCAGT GCTGGGCCAC GAGGTGGGCT ACTGCATCCG CTTTGATGAC
    TGCACCGACC AGCTGGCCAC GAGAATTAAG TTTCTTACTG ATGGAATGCT GGTCAGGGAA
    ATGATGGTTG ATCCGTTGTT AACAAAATAT AGTGTCATCA TGCTGGATGA AGCCCACGAG
    AGGACCTTGT ACACTGACAT TGCCATTGGC TTGCTAAAAA AGATTCAGAA AAAGCGAGGG
    GATCTTCGAT TGATTGTAGC TTCAGCCACT CTGGATGCAG ACAAATTCCG GGATTTCTTT
    AATCAAAATG AAACCAGTGA TCCAGCAAGG GATACATGTG TGATCCTTAC AGTGGAAGGG
    AGAACATTTC CGGTGGATAT CTTTTATCTA CAAAGTCCTG TTCCAGATTA TATCAAATCA
    ACTGTCGAAA CTGTGGTGAA AATTCACCAG ACAGAGGGAG ACGGAGACGT TTTAGCATTT
    CTTACTGGCC AGGAAGAGGT AGAAACTGTT GTGTCGATGC TCATCGAGCA GGCTCGAGCA
    CTAGCTCGCA CTGGGATGAA GAGACACCTC CGAGTTCTCC CCATGTATGC AGGACTGCCT
    TCCTTTGAGC AAATGAAAGT GTTTGAAAGG GTGTCACGCA GTGTCAGAAA GGTGATAGTG
    GCCACCAATG TGGCAGAAAC CTCTATCACA ATCAGCGGCA TTGTGTATGT GATCGACTGT
    GGCTTTGTGA AACTCCGAGC CTACAATCCC AGGACAGCTA TTGAATGCTT GGTGGTGGTG
    CCAGTCTCCC AGGCATCAGC TAATCAGCGA GCAGGACGTG GTGGTCGTAG TCGCTCGGGA
    AAATGTTATC GCCTTTATAC AGAGGAAGCC TTTGACAAGT TGCCTCAGTC TACGGTTCCT
    GAGATGCAGC GTAGTAATTT GGCACCTGTC ATCCTGCAGC TGAAAGCACT AGGAATTGAC
    AATGTCCTCA GGTTCCACTT CATGTCGGTC ATGTGTTGTG TGTCTGCTTT GCAACCAGGC
    CCTGTGCAGG ACGTAGGGGA AAACAGCCAG ACAAGCCCCC TCCAGCACAG TCGATGGTTC
    AAGCCTTGGA GTTACTGTAT GCTCTGGGAG GTCTGGACAA AGACTGTCGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71097
    Clone ID Related Accession (Same CDS sequence) XM_011528974.2 , XM_011528974.1
    Accession Version XM_011528974.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product probable ATP-dependent RNA helicase DHX35 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGCTGCGC CCGTGGGACC GGTGAAGTTC TGGCGACCCG GTACAGAGGG GCCAGGTGTA 
    AGCATCTCTG AAGAGAGACA AAGTCTGGCT GAAAACTCTG GGACAACGGT TGTTTACAAC
    CCTTATGCTG CCCTTTCCAT AGAGCAGCAG AGGCAGAAGC TGCCGGTATT CAAGCTTAGG
    AATCATATTT TATACTTGAT AGAAAATTAT CAGACAGTGG TGATTGTTGG TGAAACAGGA
    TGTGGGAAGA GCACACAGAT TCCTCAGTAC CTTGCAGAAG CCGGCTGGAC AGCTGAAGGA
    AGAGTGGTAG GAGTGACCCA GCCTCGAAGA GTGGCTGCTG TTACAGTTGC AGGGAGAGTA
    GCTGAAGAAA GGGGTGCAGT GCTGGGCCAC GAGGTGGGCT ACTGCATCCG CTTTGATGAC
    TGCACCGACC AGCTGGCCAC GAGAATTAAG TTTCTTACTG ATGGAATGCT GGTCAGGGAA
    ATGATGGTTG ATCCGTTGTT AACAAAATAT AGTGTCATCA TGCTGGATGA AGCCCACGAG
    AGGACCTTGT ACACTGACAT TGCCATTGGC TTGCTAAAAA AGATTCAGAA AAAGCGAGGG
    GATCTTCGAT TGATTGTAGC TTCAGCCACT CTGGATGCAG ACAAATTCCG GGATTTCTTT
    AATCAAAATG AAACCAGTGA TCCAGCAAGG GATACATGTG TGATCCTTAC AGTGGAAGGG
    AGAACATTTC CGGTGGATAT CTTTTATCTA CAAAGTCCTG TTCCAGATTA TATCAAATCA
    ACTGTCGAAA CTGTGGTGAA AATTCACCAG ACAGAGGGAG ACGGAGACGT TTTAGCATTT
    CTTACTGGCC AGGAAGAGGT AGAAACTGTT GTGTCGATGC TCATCGAGCA GGCTCGAGCA
    CTAGCTCGCA CTGGGATGAA GAGACACCTC CGAGTTCTCC CCATGTATGC AGGACTGCCT
    TCCTTTGAGC AAATGAAAGT GTTTGAAAGG GTGTCACGCA GTGTCAGAAA GGTGATAGTG
    GCCACCAATG TGGCAGAAAC CTCTATCACA ATCAGCGGCA TTGTGTATGT GATCGACTGT
    GGCTTTGTGA AACTCCGAGC CTACAATCCC AGGACAGCTA TTGAATGCTT GGTGGTGGTG
    CCAGTCTCCC AGGCATCAGC TAATCAGCGA GCAGGACGTG GTGGTCGTAG TCGCTCGGGA
    AAATGTTATC GCCTTTATAC AGAGGAAGCC TTTGACAAGT TGCCTCAGTC TACGGTTCCT
    GAGATGCAGC GTAGTAATTT GGCACCTGTC ATCCTGCAGC TGAAAGCACT AGGAATTGAC
    AATGTCCTCA GGTTCCACTT CATGTCGGTC ATGTGTTGTG TGTCTGCTTT GCAACCAGGC
    CCTGTGCAGG ACGTAGGGGA AAACAGCCAG ACAAGCCCCC TCCAGCACAG TCGATGGTTC
    AAGCCTTGGA GTTACTGTAT GCTCTGGGAG GTCTGGACAA AGACTGTCGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011527276.1
    CDS31..1524
    Translation

    Target ORF information:

    RefSeq Version XM_011528974.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DEAH-box helicase 35 (DHX35), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011528974.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGCTGCGC CCGTGGGACC GGTGAAGTTC TGGCGACCCG GTACAGAGGG GCCAGGTGTA 
    AGCATCTCTG AAGAGAGACA AAGTCTGGCT GAAAACTCTG GGACAACGGT TGTTTACAAC
    CCTTATGCTG CCCTTTCCAT AGAGCAGCAG AGGCAGAAGC TGCCGGTATT CAAGCTTAGG
    AATCATATTT TATACTTGAT AGAAAATTAT CAGACAGTGG TGATTGTTGG TGAAACAGGA
    TGTGGGAAGA GCACACAGAT TCCTCAGTAC CTTGCAGAAG CCGGCTGGAC AGCTGAAGGA
    AGAGTGGTAG GAGTGACCCA GCCTCGAAGA GTGGCTGCTG TTACAGTTGC AGGGAGAGTA
    GCTGAAGAAA GGGGTGCAGT GCTGGGCCAC GAGGTGGGCT ACTGCATCCG CTTTGATGAC
    TGCACCGACC AGCTGGCCAC GAGAATTAAG TTTCTTACTG ATGGAATGCT GGTCAGGGAA
    ATGATGGTTG ATCCGTTGTT AACAAAATAT AGTGTCATCA TGCTGGATGA AGCCCACGAG
    AGGACCTTGT ACACTGACAT TGCCATTGGC TTGCTAAAAA AGATTCAGAA AAAGCGAGGG
    GATCTTCGAT TGATTGTAGC TTCAGCCACT CTGGATGCAG ACAAATTCCG GGATTTCTTT
    AATCAAAATG AAACCAGTGA TCCAGCAAGG GATACATGTG TGATCCTTAC AGTGGAAGGG
    AGAACATTTC CGGTGGATAT CTTTTATCTA CAAAGTCCTG TTCCAGATTA TATCAAATCA
    ACTGTCGAAA CTGTGGTGAA AATTCACCAG ACAGAGGGAG ACGGAGACGT TTTAGCATTT
    CTTACTGGCC AGGAAGAGGT AGAAACTGTT GTGTCGATGC TCATCGAGCA GGCTCGAGCA
    CTAGCTCGCA CTGGGATGAA GAGACACCTC CGAGTTCTCC CCATGTATGC AGGACTGCCT
    TCCTTTGAGC AAATGAAAGT GTTTGAAAGG GTGTCACGCA GTGTCAGAAA GGTGATAGTG
    GCCACCAATG TGGCAGAAAC CTCTATCACA ATCAGCGGCA TTGTGTATGT GATCGACTGT
    GGCTTTGTGA AACTCCGAGC CTACAATCCC AGGACAGCTA TTGAATGCTT GGTGGTGGTG
    CCAGTCTCCC AGGCATCAGC TAATCAGCGA GCAGGACGTG GTGGTCGTAG TCGCTCGGGA
    AAATGTTATC GCCTTTATAC AGAGGAAGCC TTTGACAAGT TGCCTCAGTC TACGGTTCCT
    GAGATGCAGC GTAGTAATTT GGCACCTGTC ATCCTGCAGC TGAAAGCACT AGGAATTGAC
    AATGTCCTCA GGTTCCACTT CATGTCGGTC ATGTGTTGTG TGTCTGCTTT GCAACCAGGC
    CCTGTGCAGG ACGTAGGGGA AAACAGCCAG ACAAGCCCCC TCCAGCACAG TCGATGGTTC
    AAGCCTTGGA GTTACTGTAT GCTCTGGGAG GTCTGGACAA AGACTGTCGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu05033
    Clone ID Related Accession (Same CDS sequence) NM_001190809.2 , NM_001190809.1
    Accession Version NM_001190809.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2019bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-08
    Organism Homo sapiens(human)
    Product probable ATP-dependent RNA helicase DHX35 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA725122.1 and BC033453.1. On Jun 1, 2019 this sequence version replaced NM_001190809.1. Transcript Variant: This variant (2) is missing an in-frame coding exon compared to variant 1, resulting in a shorter isoform (2) lacking an internal protein segment compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK303396.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGCTGCGC CCGTGGGACC GGTGAAGTTC TGGCGACCCG GTACAGAGGG GCCAGGTGTA 
    AGCATCTCTG AAGAGAGACA AAGTCTGGCT GAAAACTCTG GGACAACGGT TGTTTACAAC
    CCTTATGCTG CCCTTTCCAT AGAGCAGCAG AGGCAGAAGC TGCCGGTATT CAAGTACCTT
    GCAGAAGCCG GCTGGACAGC TGAAGGAAGA GTGGTAGGAG TGACCCAGCC TCGAAGAGTG
    GCTGCTGTTA CAGTTGCAGG GAGAGTAGCT GAAGAAAGGG GTGCAGTGCT GGGCCACGAG
    GTGGGCTACT GCATCCGCTT TGATGACTGC ACCGACCAGC TGGCCACGAG AATTAAGTTT
    CTTACTGATG GAATGCTGGT CAGGGAAATG ATGGTTGATC CGTTGTTAAC AAAATATAGT
    GTCATCATGC TGGATGAAGC CCACGAGAGG ACCTTGTACA CTGACATTGC CATTGGCTTG
    CTAAAAAAGA TTCAGAAAAA GCGAGGGGAT CTTCGATTGA TTGTAGCTTC AGCCACTCTG
    GATGCAGACA AATTCCGGGA TTTCTTTAAT CAAAATGAAA CCAGTGATCC AGCAAGGGAT
    ACATGTGTGA TCCTTACAGT GGAAGGGAGA ACATTTCCGG TGGATATCTT TTATCTACAA
    AGTCCTGTTC CAGATTATAT CAAATCAACT GTCGAAACTG TGGTGAAAAT TCACCAGACA
    GAGGGAGACG GAGACGTTTT AGCATTTCTT ACTGGCCAGG AAGAGGTAGA AACTGTTGTG
    TCGATGCTCA TCGAGCAGGC TCGAGCACTA GCTCGCACTG GGATGAAGAG ACACCTCCGA
    GTTCTCCCCA TGTATGCAGG ACTGCCTTCC TTTGAGCAAA TGAAAGTGTT TGAAAGGGTG
    TCACGCAGTG TCAGAAAGGT GATAGTGGCC ACCAATGTGG CAGAAACCTC TATCACAATC
    AGCGGCATTG TGTATGTGAT CGACTGTGGC TTTGTGAAAC TCCGAGCCTA CAATCCCAGG
    ACAGCTATTG AATGCTTGGT GGTGGTGCCA GTCTCCCAGG CATCAGCTAA TCAGCGAGCA
    GGACGTGGTG GTCGTAGTCG CTCGGGAAAA TGTTATCGCC TTTATACAGA GGAAGCCTTT
    GACAAGTTGC CTCAGTCTAC GGTTCCTGAG ATGCAGCGTA GTAATTTGGC ACCTGTCATC
    CTGCAGCTGA AAGCACTAGG AATTGACAAT GTCCTCAGGT TCCACTTCAT GTCGCCCCCT
    CCAGCACAGT CGATGGTTCA AGCCTTGGAG TTACTGTATG CTCTGGGAGG TCTGGACAAA
    GACTGTCGCC TAACTGAACC GCTTGGCATG AGAATTGCAG AGTTTCCTTT GAATCCCATG
    TTTGCCAAAA TGCTGCTTGA ATCAGGAAAC TTCGGCTGTT CTCAGGAAAT TCTAAGCATC
    GCTGCCATGA TGCAGATCCA GAATATCTTT GTGGTCCCCC CAAACCAGAA GTCTCACGCA
    ATTCGAGTGC ACCGTAAATT TGCTGTGGAG GAGGGCGACC ACCTCACTAT GCTCAATATA
    TATGAAGCAT TTATCAAACA CAATAAGGAC TCTAAATGGT GTCAGGAACA TTTCCTGAAT
    TACAAGGGTC TTGTCAGAGC TGCGACTGTA AGAGAACAAT TGAAAAAGCT TCTTGTCAAG
    TTTCAAGTGC CCAGGAAGTC TAGTGAAGGT GACCCGGATC TGGTTCTGAG GTGCATTGTC
    TCCGGCTTCT TCGCCAATGC AGCGAGGTTT CATTCTACTG GAGCTTATAG GACCATCCGT
    GATGACCATG AGCTGCACAT ACACCCTGCG TCAGTCCTCT ATGCAGAGAA GCCGCCTCGC
    TGGGTCATCT ATAACGAAGT TATACAGACC TCCAAGTACT ACATGAGAGA TGTGACTGCC
    ATTGAATCGG CCTGGCTGTT GGAGCTGGCT CCACACTTTT ATCAACAAGG AACGCACCTG
    TCTCTGAAAG CCAAAAGGGC CAAGGTCCAG GACCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001177738.1
    CDS25..2043
    Translation

    Target ORF information:

    RefSeq Version NM_001190809.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DEAH-box helicase 35 (DHX35), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001190809.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGCTGCGC CCGTGGGACC GGTGAAGTTC TGGCGACCCG GTACAGAGGG GCCAGGTGTA 
    AGCATCTCTG AAGAGAGACA AAGTCTGGCT GAAAACTCTG GGACAACGGT TGTTTACAAC
    CCTTATGCTG CCCTTTCCAT AGAGCAGCAG AGGCAGAAGC TGCCGGTATT CAAGTACCTT
    GCAGAAGCCG GCTGGACAGC TGAAGGAAGA GTGGTAGGAG TGACCCAGCC TCGAAGAGTG
    GCTGCTGTTA CAGTTGCAGG GAGAGTAGCT GAAGAAAGGG GTGCAGTGCT GGGCCACGAG
    GTGGGCTACT GCATCCGCTT TGATGACTGC ACCGACCAGC TGGCCACGAG AATTAAGTTT
    CTTACTGATG GAATGCTGGT CAGGGAAATG ATGGTTGATC CGTTGTTAAC AAAATATAGT
    GTCATCATGC TGGATGAAGC CCACGAGAGG ACCTTGTACA CTGACATTGC CATTGGCTTG
    CTAAAAAAGA TTCAGAAAAA GCGAGGGGAT CTTCGATTGA TTGTAGCTTC AGCCACTCTG
    GATGCAGACA AATTCCGGGA TTTCTTTAAT CAAAATGAAA CCAGTGATCC AGCAAGGGAT
    ACATGTGTGA TCCTTACAGT GGAAGGGAGA ACATTTCCGG TGGATATCTT TTATCTACAA
    AGTCCTGTTC CAGATTATAT CAAATCAACT GTCGAAACTG TGGTGAAAAT TCACCAGACA
    GAGGGAGACG GAGACGTTTT AGCATTTCTT ACTGGCCAGG AAGAGGTAGA AACTGTTGTG
    TCGATGCTCA TCGAGCAGGC TCGAGCACTA GCTCGCACTG GGATGAAGAG ACACCTCCGA
    GTTCTCCCCA TGTATGCAGG ACTGCCTTCC TTTGAGCAAA TGAAAGTGTT TGAAAGGGTG
    TCACGCAGTG TCAGAAAGGT GATAGTGGCC ACCAATGTGG CAGAAACCTC TATCACAATC
    AGCGGCATTG TGTATGTGAT CGACTGTGGC TTTGTGAAAC TCCGAGCCTA CAATCCCAGG
    ACAGCTATTG AATGCTTGGT GGTGGTGCCA GTCTCCCAGG CATCAGCTAA TCAGCGAGCA
    GGACGTGGTG GTCGTAGTCG CTCGGGAAAA TGTTATCGCC TTTATACAGA GGAAGCCTTT
    GACAAGTTGC CTCAGTCTAC GGTTCCTGAG ATGCAGCGTA GTAATTTGGC ACCTGTCATC
    CTGCAGCTGA AAGCACTAGG AATTGACAAT GTCCTCAGGT TCCACTTCAT GTCGCCCCCT
    CCAGCACAGT CGATGGTTCA AGCCTTGGAG TTACTGTATG CTCTGGGAGG TCTGGACAAA
    GACTGTCGCC TAACTGAACC GCTTGGCATG AGAATTGCAG AGTTTCCTTT GAATCCCATG
    TTTGCCAAAA TGCTGCTTGA ATCAGGAAAC TTCGGCTGTT CTCAGGAAAT TCTAAGCATC
    GCTGCCATGA TGCAGATCCA GAATATCTTT GTGGTCCCCC CAAACCAGAA GTCTCACGCA
    ATTCGAGTGC ACCGTAAATT TGCTGTGGAG GAGGGCGACC ACCTCACTAT GCTCAATATA
    TATGAAGCAT TTATCAAACA CAATAAGGAC TCTAAATGGT GTCAGGAACA TTTCCTGAAT
    TACAAGGGTC TTGTCAGAGC TGCGACTGTA AGAGAACAAT TGAAAAAGCT TCTTGTCAAG
    TTTCAAGTGC CCAGGAAGTC TAGTGAAGGT GACCCGGATC TGGTTCTGAG GTGCATTGTC
    TCCGGCTTCT TCGCCAATGC AGCGAGGTTT CATTCTACTG GAGCTTATAG GACCATCCGT
    GATGACCATG AGCTGCACAT ACACCCTGCG TCAGTCCTCT ATGCAGAGAA GCCGCCTCGC
    TGGGTCATCT ATAACGAAGT TATACAGACC TCCAAGTACT ACATGAGAGA TGTGACTGCC
    ATTGAATCGG CCTGGCTGTT GGAGCTGGCT CCACACTTTT ATCAACAAGG AACGCACCTG
    TCTCTGAAAG CCAAAAGGGC CAAGGTCCAG GACCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu05033
    Clone ID Related Accession (Same CDS sequence) NM_001190809.2 , NM_001190809.1
    Accession Version NM_001190809.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2019bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Homo sapiens(human)
    Product probable ATP-dependent RNA helicase DHX35 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA725122.1, BC033453.1 and AW015983.1. Transcript Variant: This variant (2) is missing an in-frame coding exon compared to variant 1, resulting in a shorter isoform (2) lacking an internal protein segment compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK303396.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGCTGCGC CCGTGGGACC GGTGAAGTTC TGGCGACCCG GTACAGAGGG GCCAGGTGTA 
    AGCATCTCTG AAGAGAGACA AAGTCTGGCT GAAAACTCTG GGACAACGGT TGTTTACAAC
    CCTTATGCTG CCCTTTCCAT AGAGCAGCAG AGGCAGAAGC TGCCGGTATT CAAGTACCTT
    GCAGAAGCCG GCTGGACAGC TGAAGGAAGA GTGGTAGGAG TGACCCAGCC TCGAAGAGTG
    GCTGCTGTTA CAGTTGCAGG GAGAGTAGCT GAAGAAAGGG GTGCAGTGCT GGGCCACGAG
    GTGGGCTACT GCATCCGCTT TGATGACTGC ACCGACCAGC TGGCCACGAG AATTAAGTTT
    CTTACTGATG GAATGCTGGT CAGGGAAATG ATGGTTGATC CGTTGTTAAC AAAATATAGT
    GTCATCATGC TGGATGAAGC CCACGAGAGG ACCTTGTACA CTGACATTGC CATTGGCTTG
    CTAAAAAAGA TTCAGAAAAA GCGAGGGGAT CTTCGATTGA TTGTAGCTTC AGCCACTCTG
    GATGCAGACA AATTCCGGGA TTTCTTTAAT CAAAATGAAA CCAGTGATCC AGCAAGGGAT
    ACATGTGTGA TCCTTACAGT GGAAGGGAGA ACATTTCCGG TGGATATCTT TTATCTACAA
    AGTCCTGTTC CAGATTATAT CAAATCAACT GTCGAAACTG TGGTGAAAAT TCACCAGACA
    GAGGGAGACG GAGACGTTTT AGCATTTCTT ACTGGCCAGG AAGAGGTAGA AACTGTTGTG
    TCGATGCTCA TCGAGCAGGC TCGAGCACTA GCTCGCACTG GGATGAAGAG ACACCTCCGA
    GTTCTCCCCA TGTATGCAGG ACTGCCTTCC TTTGAGCAAA TGAAAGTGTT TGAAAGGGTG
    TCACGCAGTG TCAGAAAGGT GATAGTGGCC ACCAATGTGG CAGAAACCTC TATCACAATC
    AGCGGCATTG TGTATGTGAT CGACTGTGGC TTTGTGAAAC TCCGAGCCTA CAATCCCAGG
    ACAGCTATTG AATGCTTGGT GGTGGTGCCA GTCTCCCAGG CATCAGCTAA TCAGCGAGCA
    GGACGTGGTG GTCGTAGTCG CTCGGGAAAA TGTTATCGCC TTTATACAGA GGAAGCCTTT
    GACAAGTTGC CTCAGTCTAC GGTTCCTGAG ATGCAGCGTA GTAATTTGGC ACCTGTCATC
    CTGCAGCTGA AAGCACTAGG AATTGACAAT GTCCTCAGGT TCCACTTCAT GTCGCCCCCT
    CCAGCACAGT CGATGGTTCA AGCCTTGGAG TTACTGTATG CTCTGGGAGG TCTGGACAAA
    GACTGTCGCC TAACTGAACC GCTTGGCATG AGAATTGCAG AGTTTCCTTT GAATCCCATG
    TTTGCCAAAA TGCTGCTTGA ATCAGGAAAC TTCGGCTGTT CTCAGGAAAT TCTAAGCATC
    GCTGCCATGA TGCAGATCCA GAATATCTTT GTGGTCCCCC CAAACCAGAA GTCTCACGCA
    ATTCGAGTGC ACCGTAAATT TGCTGTGGAG GAGGGCGACC ACCTCACTAT GCTCAATATA
    TATGAAGCAT TTATCAAACA CAATAAGGAC TCTAAATGGT GTCAGGAACA TTTCCTGAAT
    TACAAGGGTC TTGTCAGAGC TGCGACTGTA AGAGAACAAT TGAAAAAGCT TCTTGTCAAG
    TTTCAAGTGC CCAGGAAGTC TAGTGAAGGT GACCCGGATC TGGTTCTGAG GTGCATTGTC
    TCCGGCTTCT TCGCCAATGC AGCGAGGTTT CATTCTACTG GAGCTTATAG GACCATCCGT
    GATGACCATG AGCTGCACAT ACACCCTGCG TCAGTCCTCT ATGCAGAGAA GCCGCCTCGC
    TGGGTCATCT ATAACGAAGT TATACAGACC TCCAAGTACT ACATGAGAGA TGTGACTGCC
    ATTGAATCGG CCTGGCTGTT GGAGCTGGCT CCACACTTTT ATCAACAAGG AACGCACCTG
    TCTCTGAAAG CCAAAAGGGC CAAGGTCCAG GACCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001177738.1
    CDS31..2049
    Translation

    Target ORF information:

    RefSeq Version NM_001190809.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DEAH-box helicase 35 (DHX35), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001190809.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGCTGCGC CCGTGGGACC GGTGAAGTTC TGGCGACCCG GTACAGAGGG GCCAGGTGTA 
    AGCATCTCTG AAGAGAGACA AAGTCTGGCT GAAAACTCTG GGACAACGGT TGTTTACAAC
    CCTTATGCTG CCCTTTCCAT AGAGCAGCAG AGGCAGAAGC TGCCGGTATT CAAGTACCTT
    GCAGAAGCCG GCTGGACAGC TGAAGGAAGA GTGGTAGGAG TGACCCAGCC TCGAAGAGTG
    GCTGCTGTTA CAGTTGCAGG GAGAGTAGCT GAAGAAAGGG GTGCAGTGCT GGGCCACGAG
    GTGGGCTACT GCATCCGCTT TGATGACTGC ACCGACCAGC TGGCCACGAG AATTAAGTTT
    CTTACTGATG GAATGCTGGT CAGGGAAATG ATGGTTGATC CGTTGTTAAC AAAATATAGT
    GTCATCATGC TGGATGAAGC CCACGAGAGG ACCTTGTACA CTGACATTGC CATTGGCTTG
    CTAAAAAAGA TTCAGAAAAA GCGAGGGGAT CTTCGATTGA TTGTAGCTTC AGCCACTCTG
    GATGCAGACA AATTCCGGGA TTTCTTTAAT CAAAATGAAA CCAGTGATCC AGCAAGGGAT
    ACATGTGTGA TCCTTACAGT GGAAGGGAGA ACATTTCCGG TGGATATCTT TTATCTACAA
    AGTCCTGTTC CAGATTATAT CAAATCAACT GTCGAAACTG TGGTGAAAAT TCACCAGACA
    GAGGGAGACG GAGACGTTTT AGCATTTCTT ACTGGCCAGG AAGAGGTAGA AACTGTTGTG
    TCGATGCTCA TCGAGCAGGC TCGAGCACTA GCTCGCACTG GGATGAAGAG ACACCTCCGA
    GTTCTCCCCA TGTATGCAGG ACTGCCTTCC TTTGAGCAAA TGAAAGTGTT TGAAAGGGTG
    TCACGCAGTG TCAGAAAGGT GATAGTGGCC ACCAATGTGG CAGAAACCTC TATCACAATC
    AGCGGCATTG TGTATGTGAT CGACTGTGGC TTTGTGAAAC TCCGAGCCTA CAATCCCAGG
    ACAGCTATTG AATGCTTGGT GGTGGTGCCA GTCTCCCAGG CATCAGCTAA TCAGCGAGCA
    GGACGTGGTG GTCGTAGTCG CTCGGGAAAA TGTTATCGCC TTTATACAGA GGAAGCCTTT
    GACAAGTTGC CTCAGTCTAC GGTTCCTGAG ATGCAGCGTA GTAATTTGGC ACCTGTCATC
    CTGCAGCTGA AAGCACTAGG AATTGACAAT GTCCTCAGGT TCCACTTCAT GTCGCCCCCT
    CCAGCACAGT CGATGGTTCA AGCCTTGGAG TTACTGTATG CTCTGGGAGG TCTGGACAAA
    GACTGTCGCC TAACTGAACC GCTTGGCATG AGAATTGCAG AGTTTCCTTT GAATCCCATG
    TTTGCCAAAA TGCTGCTTGA ATCAGGAAAC TTCGGCTGTT CTCAGGAAAT TCTAAGCATC
    GCTGCCATGA TGCAGATCCA GAATATCTTT GTGGTCCCCC CAAACCAGAA GTCTCACGCA
    ATTCGAGTGC ACCGTAAATT TGCTGTGGAG GAGGGCGACC ACCTCACTAT GCTCAATATA
    TATGAAGCAT TTATCAAACA CAATAAGGAC TCTAAATGGT GTCAGGAACA TTTCCTGAAT
    TACAAGGGTC TTGTCAGAGC TGCGACTGTA AGAGAACAAT TGAAAAAGCT TCTTGTCAAG
    TTTCAAGTGC CCAGGAAGTC TAGTGAAGGT GACCCGGATC TGGTTCTGAG GTGCATTGTC
    TCCGGCTTCT TCGCCAATGC AGCGAGGTTT CATTCTACTG GAGCTTATAG GACCATCCGT
    GATGACCATG AGCTGCACAT ACACCCTGCG TCAGTCCTCT ATGCAGAGAA GCCGCCTCGC
    TGGGTCATCT ATAACGAAGT TATACAGACC TCCAAGTACT ACATGAGAGA TGTGACTGCC
    ATTGAATCGG CCTGGCTGTT GGAGCTGGCT CCACACTTTT ATCAACAAGG AACGCACCTG
    TCTCTGAAAG CCAAAAGGGC CAAGGTCCAG GACCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu32249
    Clone ID Related Accession (Same CDS sequence) NM_021931.4 , NM_021931.3
    Accession Version NM_021931.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-06
    Organism Homo sapiens(human)
    Product probable ATP-dependent RNA helicase DHX35 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA725122.1 and BC033453.1. On Nov 22, 2018 this sequence version replaced NM_021931.3. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC059369.1, SRR1803615.23579.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000252011.8/ ENSP00000252011.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCTGCGC CCGTGGGACC GGTGAAGTTC TGGCGACCCG GTACAGAGGG GCCAGGTGTA 
    AGCATCTCTG AAGAGAGACA AAGTCTGGCT GAAAACTCTG GGACAACGGT TGTTTACAAC
    CCTTATGCTG CCCTTTCCAT AGAGCAGCAG AGGCAGAAGC TGCCGGTATT CAAGCTTAGG
    AATCATATTT TATACTTGAT AGAAAATTAT CAGACAGTGG TGATTGTTGG TGAAACAGGA
    TGTGGGAAGA GCACACAGAT TCCTCAGTAC CTTGCAGAAG CCGGCTGGAC AGCTGAAGGA
    AGAGTGGTAG GAGTGACCCA GCCTCGAAGA GTGGCTGCTG TTACAGTTGC AGGGAGAGTA
    GCTGAAGAAA GGGGTGCAGT GCTGGGCCAC GAGGTGGGCT ACTGCATCCG CTTTGATGAC
    TGCACCGACC AGCTGGCCAC GAGAATTAAG TTTCTTACTG ATGGAATGCT GGTCAGGGAA
    ATGATGGTTG ATCCGTTGTT AACAAAATAT AGTGTCATCA TGCTGGATGA AGCCCACGAG
    AGGACCTTGT ACACTGACAT TGCCATTGGC TTGCTAAAAA AGATTCAGAA AAAGCGAGGG
    GATCTTCGAT TGATTGTAGC TTCAGCCACT CTGGATGCAG ACAAATTCCG GGATTTCTTT
    AATCAAAATG AAACCAGTGA TCCAGCAAGG GATACATGTG TGATCCTTAC AGTGGAAGGG
    AGAACATTTC CGGTGGATAT CTTTTATCTA CAAAGTCCTG TTCCAGATTA TATCAAATCA
    ACTGTCGAAA CTGTGGTGAA AATTCACCAG ACAGAGGGAG ACGGAGACGT TTTAGCATTT
    CTTACTGGCC AGGAAGAGGT AGAAACTGTT GTGTCGATGC TCATCGAGCA GGCTCGAGCA
    CTAGCTCGCA CTGGGATGAA GAGACACCTC CGAGTTCTCC CCATGTATGC AGGACTGCCT
    TCCTTTGAGC AAATGAAAGT GTTTGAAAGG GTGTCACGCA GTGTCAGAAA GGTGATAGTG
    GCCACCAATG TGGCAGAAAC CTCTATCACA ATCAGCGGCA TTGTGTATGT GATCGACTGT
    GGCTTTGTGA AACTCCGAGC CTACAATCCC AGGACAGCTA TTGAATGCTT GGTGGTGGTG
    CCAGTCTCCC AGGCATCAGC TAATCAGCGA GCAGGACGTG GTGGTCGTAG TCGCTCGGGA
    AAATGTTATC GCCTTTATAC AGAGGAAGCC TTTGACAAGT TGCCTCAGTC TACGGTTCCT
    GAGATGCAGC GTAGTAATTT GGCACCTGTC ATCCTGCAGC TGAAAGCACT AGGAATTGAC
    AATGTCCTCA GGTTCCACTT CATGTCGCCC CCTCCAGCAC AGTCGATGGT TCAAGCCTTG
    GAGTTACTGT ATGCTCTGGG AGGTCTGGAC AAAGACTGTC GCCTAACTGA ACCGCTTGGC
    ATGAGAATTG CAGAGTTTCC TTTGAATCCC ATGTTTGCCA AAATGCTGCT TGAATCAGGA
    AACTTCGGCT GTTCTCAGGA AATTCTAAGC ATCGCTGCCA TGATGCAGAT CCAGAATATC
    TTTGTGGTCC CCCCAAACCA GAAGTCTCAC GCAATTCGAG TGCACCGTAA ATTTGCTGTG
    GAGGAGGGCG ACCACCTCAC TATGCTCAAT ATATATGAAG CATTTATCAA ACACAATAAG
    GACTCTAAAT GGTGTCAGGA ACATTTCCTG AATTACAAGG GTCTTGTCAG AGCTGCGACT
    GTAAGAGAAC AATTGAAAAA GCTTCTTGTC AAGTTTCAAG TGCCCAGGAA GTCTAGTGAA
    GGTGACCCGG ATCTGGTTCT GAGGTGCATT GTCTCCGGCT TCTTCGCCAA TGCAGCGAGG
    TTTCATTCTA CTGGAGCTTA TAGGACCATC CGTGATGACC ATGAGCTGCA CATACACCCT
    GCGTCAGTCC TCTATGCAGA GAAGCCGCCT CGCTGGGTCA TCTATAACGA AGTTATACAG
    ACCTCCAAGT ACTACATGAG AGATGTGACT GCCATTGAAT CGGCCTGGCT GTTGGAGCTG
    GCTCCACACT TTTATCAACA AGGAACGCAC CTGTCTCTGA AAGCCAAAAG GGCCAAGGTC
    CAGGACCCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_068750.2
    CDS25..2136
    Translation

    Target ORF information:

    RefSeq Version NM_021931.4
    Organism Homo sapiens(human)
    Definition Homo sapiens DEAH-box helicase 35 (DHX35), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_021931.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCTGCGC CCGTGGGACC GGTGAAGTTC TGGCGACCCG GTACAGAGGG GCCAGGTGTA 
    AGCATCTCTG AAGAGAGACA AAGTCTGGCT GAAAACTCTG GGACAACGGT TGTTTACAAC
    CCTTATGCTG CCCTTTCCAT AGAGCAGCAG AGGCAGAAGC TGCCGGTATT CAAGCTTAGG
    AATCATATTT TATACTTGAT AGAAAATTAT CAGACAGTGG TGATTGTTGG TGAAACAGGA
    TGTGGGAAGA GCACACAGAT TCCTCAGTAC CTTGCAGAAG CCGGCTGGAC AGCTGAAGGA
    AGAGTGGTAG GAGTGACCCA GCCTCGAAGA GTGGCTGCTG TTACAGTTGC AGGGAGAGTA
    GCTGAAGAAA GGGGTGCAGT GCTGGGCCAC GAGGTGGGCT ACTGCATCCG CTTTGATGAC
    TGCACCGACC AGCTGGCCAC GAGAATTAAG TTTCTTACTG ATGGAATGCT GGTCAGGGAA
    ATGATGGTTG ATCCGTTGTT AACAAAATAT AGTGTCATCA TGCTGGATGA AGCCCACGAG
    AGGACCTTGT ACACTGACAT TGCCATTGGC TTGCTAAAAA AGATTCAGAA AAAGCGAGGG
    GATCTTCGAT TGATTGTAGC TTCAGCCACT CTGGATGCAG ACAAATTCCG GGATTTCTTT
    AATCAAAATG AAACCAGTGA TCCAGCAAGG GATACATGTG TGATCCTTAC AGTGGAAGGG
    AGAACATTTC CGGTGGATAT CTTTTATCTA CAAAGTCCTG TTCCAGATTA TATCAAATCA
    ACTGTCGAAA CTGTGGTGAA AATTCACCAG ACAGAGGGAG ACGGAGACGT TTTAGCATTT
    CTTACTGGCC AGGAAGAGGT AGAAACTGTT GTGTCGATGC TCATCGAGCA GGCTCGAGCA
    CTAGCTCGCA CTGGGATGAA GAGACACCTC CGAGTTCTCC CCATGTATGC AGGACTGCCT
    TCCTTTGAGC AAATGAAAGT GTTTGAAAGG GTGTCACGCA GTGTCAGAAA GGTGATAGTG
    GCCACCAATG TGGCAGAAAC CTCTATCACA ATCAGCGGCA TTGTGTATGT GATCGACTGT
    GGCTTTGTGA AACTCCGAGC CTACAATCCC AGGACAGCTA TTGAATGCTT GGTGGTGGTG
    CCAGTCTCCC AGGCATCAGC TAATCAGCGA GCAGGACGTG GTGGTCGTAG TCGCTCGGGA
    AAATGTTATC GCCTTTATAC AGAGGAAGCC TTTGACAAGT TGCCTCAGTC TACGGTTCCT
    GAGATGCAGC GTAGTAATTT GGCACCTGTC ATCCTGCAGC TGAAAGCACT AGGAATTGAC
    AATGTCCTCA GGTTCCACTT CATGTCGCCC CCTCCAGCAC AGTCGATGGT TCAAGCCTTG
    GAGTTACTGT ATGCTCTGGG AGGTCTGGAC AAAGACTGTC GCCTAACTGA ACCGCTTGGC
    ATGAGAATTG CAGAGTTTCC TTTGAATCCC ATGTTTGCCA AAATGCTGCT TGAATCAGGA
    AACTTCGGCT GTTCTCAGGA AATTCTAAGC ATCGCTGCCA TGATGCAGAT CCAGAATATC
    TTTGTGGTCC CCCCAAACCA GAAGTCTCAC GCAATTCGAG TGCACCGTAA ATTTGCTGTG
    GAGGAGGGCG ACCACCTCAC TATGCTCAAT ATATATGAAG CATTTATCAA ACACAATAAG
    GACTCTAAAT GGTGTCAGGA ACATTTCCTG AATTACAAGG GTCTTGTCAG AGCTGCGACT
    GTAAGAGAAC AATTGAAAAA GCTTCTTGTC AAGTTTCAAG TGCCCAGGAA GTCTAGTGAA
    GGTGACCCGG ATCTGGTTCT GAGGTGCATT GTCTCCGGCT TCTTCGCCAA TGCAGCGAGG
    TTTCATTCTA CTGGAGCTTA TAGGACCATC CGTGATGACC ATGAGCTGCA CATACACCCT
    GCGTCAGTCC TCTATGCAGA GAAGCCGCCT CGCTGGGTCA TCTATAACGA AGTTATACAG
    ACCTCCAAGT ACTACATGAG AGATGTGACT GCCATTGAAT CGGCCTGGCT GTTGGAGCTG
    GCTCCACACT TTTATCAACA AGGAACGCAC CTGTCTCTGA AAGCCAAAAG GGCCAAGGTC
    CAGGACCCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu32249
    Clone ID Related Accession (Same CDS sequence) NM_021931.4 , NM_021931.3
    Accession Version NM_021931.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-01
    Organism Homo sapiens(human)
    Product probable ATP-dependent RNA helicase DHX35 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA725122.1, BC033453.1 and AW015983.1. On Jun 30, 2010 this sequence version replaced NM_021931.2. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1803615.23579.1, BC059369.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCTGCGC CCGTGGGACC GGTGAAGTTC TGGCGACCCG GTACAGAGGG GCCAGGTGTA 
    AGCATCTCTG AAGAGAGACA AAGTCTGGCT GAAAACTCTG GGACAACGGT TGTTTACAAC
    CCTTATGCTG CCCTTTCCAT AGAGCAGCAG AGGCAGAAGC TGCCGGTATT CAAGCTTAGG
    AATCATATTT TATACTTGAT AGAAAATTAT CAGACAGTGG TGATTGTTGG TGAAACAGGA
    TGTGGGAAGA GCACACAGAT TCCTCAGTAC CTTGCAGAAG CCGGCTGGAC AGCTGAAGGA
    AGAGTGGTAG GAGTGACCCA GCCTCGAAGA GTGGCTGCTG TTACAGTTGC AGGGAGAGTA
    GCTGAAGAAA GGGGTGCAGT GCTGGGCCAC GAGGTGGGCT ACTGCATCCG CTTTGATGAC
    TGCACCGACC AGCTGGCCAC GAGAATTAAG TTTCTTACTG ATGGAATGCT GGTCAGGGAA
    ATGATGGTTG ATCCGTTGTT AACAAAATAT AGTGTCATCA TGCTGGATGA AGCCCACGAG
    AGGACCTTGT ACACTGACAT TGCCATTGGC TTGCTAAAAA AGATTCAGAA AAAGCGAGGG
    GATCTTCGAT TGATTGTAGC TTCAGCCACT CTGGATGCAG ACAAATTCCG GGATTTCTTT
    AATCAAAATG AAACCAGTGA TCCAGCAAGG GATACATGTG TGATCCTTAC AGTGGAAGGG
    AGAACATTTC CGGTGGATAT CTTTTATCTA CAAAGTCCTG TTCCAGATTA TATCAAATCA
    ACTGTCGAAA CTGTGGTGAA AATTCACCAG ACAGAGGGAG ACGGAGACGT TTTAGCATTT
    CTTACTGGCC AGGAAGAGGT AGAAACTGTT GTGTCGATGC TCATCGAGCA GGCTCGAGCA
    CTAGCTCGCA CTGGGATGAA GAGACACCTC CGAGTTCTCC CCATGTATGC AGGACTGCCT
    TCCTTTGAGC AAATGAAAGT GTTTGAAAGG GTGTCACGCA GTGTCAGAAA GGTGATAGTG
    GCCACCAATG TGGCAGAAAC CTCTATCACA ATCAGCGGCA TTGTGTATGT GATCGACTGT
    GGCTTTGTGA AACTCCGAGC CTACAATCCC AGGACAGCTA TTGAATGCTT GGTGGTGGTG
    CCAGTCTCCC AGGCATCAGC TAATCAGCGA GCAGGACGTG GTGGTCGTAG TCGCTCGGGA
    AAATGTTATC GCCTTTATAC AGAGGAAGCC TTTGACAAGT TGCCTCAGTC TACGGTTCCT
    GAGATGCAGC GTAGTAATTT GGCACCTGTC ATCCTGCAGC TGAAAGCACT AGGAATTGAC
    AATGTCCTCA GGTTCCACTT CATGTCGCCC CCTCCAGCAC AGTCGATGGT TCAAGCCTTG
    GAGTTACTGT ATGCTCTGGG AGGTCTGGAC AAAGACTGTC GCCTAACTGA ACCGCTTGGC
    ATGAGAATTG CAGAGTTTCC TTTGAATCCC ATGTTTGCCA AAATGCTGCT TGAATCAGGA
    AACTTCGGCT GTTCTCAGGA AATTCTAAGC ATCGCTGCCA TGATGCAGAT CCAGAATATC
    TTTGTGGTCC CCCCAAACCA GAAGTCTCAC GCAATTCGAG TGCACCGTAA ATTTGCTGTG
    GAGGAGGGCG ACCACCTCAC TATGCTCAAT ATATATGAAG CATTTATCAA ACACAATAAG
    GACTCTAAAT GGTGTCAGGA ACATTTCCTG AATTACAAGG GTCTTGTCAG AGCTGCGACT
    GTAAGAGAAC AATTGAAAAA GCTTCTTGTC AAGTTTCAAG TGCCCAGGAA GTCTAGTGAA
    GGTGACCCGG ATCTGGTTCT GAGGTGCATT GTCTCCGGCT TCTTCGCCAA TGCAGCGAGG
    TTTCATTCTA CTGGAGCTTA TAGGACCATC CGTGATGACC ATGAGCTGCA CATACACCCT
    GCGTCAGTCC TCTATGCAGA GAAGCCGCCT CGCTGGGTCA TCTATAACGA AGTTATACAG
    ACCTCCAAGT ACTACATGAG AGATGTGACT GCCATTGAAT CGGCCTGGCT GTTGGAGCTG
    GCTCCACACT TTTATCAACA AGGAACGCAC CTGTCTCTGA AAGCCAAAAG GGCCAAGGTC
    CAGGACCCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_068750.2
    CDS31..2142
    Translation

    Target ORF information:

    RefSeq Version NM_021931.3
    Organism Homo sapiens(human)
    Definition Homo sapiens DEAH-box helicase 35 (DHX35), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_021931.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCTGCGC CCGTGGGACC GGTGAAGTTC TGGCGACCCG GTACAGAGGG GCCAGGTGTA 
    AGCATCTCTG AAGAGAGACA AAGTCTGGCT GAAAACTCTG GGACAACGGT TGTTTACAAC
    CCTTATGCTG CCCTTTCCAT AGAGCAGCAG AGGCAGAAGC TGCCGGTATT CAAGCTTAGG
    AATCATATTT TATACTTGAT AGAAAATTAT CAGACAGTGG TGATTGTTGG TGAAACAGGA
    TGTGGGAAGA GCACACAGAT TCCTCAGTAC CTTGCAGAAG CCGGCTGGAC AGCTGAAGGA
    AGAGTGGTAG GAGTGACCCA GCCTCGAAGA GTGGCTGCTG TTACAGTTGC AGGGAGAGTA
    GCTGAAGAAA GGGGTGCAGT GCTGGGCCAC GAGGTGGGCT ACTGCATCCG CTTTGATGAC
    TGCACCGACC AGCTGGCCAC GAGAATTAAG TTTCTTACTG ATGGAATGCT GGTCAGGGAA
    ATGATGGTTG ATCCGTTGTT AACAAAATAT AGTGTCATCA TGCTGGATGA AGCCCACGAG
    AGGACCTTGT ACACTGACAT TGCCATTGGC TTGCTAAAAA AGATTCAGAA AAAGCGAGGG
    GATCTTCGAT TGATTGTAGC TTCAGCCACT CTGGATGCAG ACAAATTCCG GGATTTCTTT
    AATCAAAATG AAACCAGTGA TCCAGCAAGG GATACATGTG TGATCCTTAC AGTGGAAGGG
    AGAACATTTC CGGTGGATAT CTTTTATCTA CAAAGTCCTG TTCCAGATTA TATCAAATCA
    ACTGTCGAAA CTGTGGTGAA AATTCACCAG ACAGAGGGAG ACGGAGACGT TTTAGCATTT
    CTTACTGGCC AGGAAGAGGT AGAAACTGTT GTGTCGATGC TCATCGAGCA GGCTCGAGCA
    CTAGCTCGCA CTGGGATGAA GAGACACCTC CGAGTTCTCC CCATGTATGC AGGACTGCCT
    TCCTTTGAGC AAATGAAAGT GTTTGAAAGG GTGTCACGCA GTGTCAGAAA GGTGATAGTG
    GCCACCAATG TGGCAGAAAC CTCTATCACA ATCAGCGGCA TTGTGTATGT GATCGACTGT
    GGCTTTGTGA AACTCCGAGC CTACAATCCC AGGACAGCTA TTGAATGCTT GGTGGTGGTG
    CCAGTCTCCC AGGCATCAGC TAATCAGCGA GCAGGACGTG GTGGTCGTAG TCGCTCGGGA
    AAATGTTATC GCCTTTATAC AGAGGAAGCC TTTGACAAGT TGCCTCAGTC TACGGTTCCT
    GAGATGCAGC GTAGTAATTT GGCACCTGTC ATCCTGCAGC TGAAAGCACT AGGAATTGAC
    AATGTCCTCA GGTTCCACTT CATGTCGCCC CCTCCAGCAC AGTCGATGGT TCAAGCCTTG
    GAGTTACTGT ATGCTCTGGG AGGTCTGGAC AAAGACTGTC GCCTAACTGA ACCGCTTGGC
    ATGAGAATTG CAGAGTTTCC TTTGAATCCC ATGTTTGCCA AAATGCTGCT TGAATCAGGA
    AACTTCGGCT GTTCTCAGGA AATTCTAAGC ATCGCTGCCA TGATGCAGAT CCAGAATATC
    TTTGTGGTCC CCCCAAACCA GAAGTCTCAC GCAATTCGAG TGCACCGTAA ATTTGCTGTG
    GAGGAGGGCG ACCACCTCAC TATGCTCAAT ATATATGAAG CATTTATCAA ACACAATAAG
    GACTCTAAAT GGTGTCAGGA ACATTTCCTG AATTACAAGG GTCTTGTCAG AGCTGCGACT
    GTAAGAGAAC AATTGAAAAA GCTTCTTGTC AAGTTTCAAG TGCCCAGGAA GTCTAGTGAA
    GGTGACCCGG ATCTGGTTCT GAGGTGCATT GTCTCCGGCT TCTTCGCCAA TGCAGCGAGG
    TTTCATTCTA CTGGAGCTTA TAGGACCATC CGTGATGACC ATGAGCTGCA CATACACCCT
    GCGTCAGTCC TCTATGCAGA GAAGCCGCCT CGCTGGGTCA TCTATAACGA AGTTATACAG
    ACCTCCAAGT ACTACATGAG AGATGTGACT GCCATTGAAT CGGCCTGGCT GTTGGAGCTG
    GCTCCACACT TTTATCAACA AGGAACGCAC CTGTCTCTGA AAGCCAAAAG GGCCAAGGTC
    CAGGACCCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71095
    Clone ID Related Accession (Same CDS sequence) XM_011528972.1 , XM_011528972.2
    Accession Version XM_011528972.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2034bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product probable ATP-dependent RNA helicase DHX35 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGCTGCGC CCGTGGGACC GGTGAAGTTC TGGCGACCCG GTACAGAGGG GCCAGGTGTA 
    AGCATCTCTG AAGAGAGACA AAGTCTGGCT GAAAACTCTG GGACAACGGT TGTTTACAAC
    CCTTATGCTG CCCTTTCCAT AGAGCAGCAG AGGCAGAAGC TGCCGGTATT CAAGCTTAGG
    AATCATATTT TATACTTGAT AGAAAATTAT CAGACAGTGG TGATTGTTGG TGAAACAGGA
    TGTGGGAAGA GCACACAGAT TCCTCAGTAC CTTGCAGAAG CCGGCTGGAC AGCTGAAGGA
    AGAGTGGTAG GAGTGACCCA GCCTCGAAGA GTGGCTGCTG TTACAGTTGC AGGGAGAGTA
    GCTGAAGAAA GGGGTGCAGT GCTGGGCCAC GAGGTGGGCT ACTGCATCCG CTTTGATGAC
    TGCACCGACC AGCTGGCCAC GAGAATTAAG TTTCTTACTG ATGGAATGCT GGTCAGGGAA
    ATGATGGTTG ATCCGTTGTT AACAAAATAT AGTGTCATCA TGCTGGATGA AGCCCACGAG
    AGGACCTTGT ACACTGACAT TGCCATTGGC TTGCTAAAAA AGATTCAGAA AAAGCGAGGG
    GATCTTCGAT TGATTGTAGC TTCAGCCACT CTGGATGCAG ACAAATTCCG GGATTTCTTT
    AATCAAAATG AAACCAGTGA TCCAGCAAGG GATACATGTG TGATCCTTAC AGTGGAAGGG
    AGAACATTTC CGGTGGATAT CTTTTATCTA CAAAGTCCTG TTCCAGATTA TATCAAATCA
    ACTGTCGAAA CTGTGGTGAA AATTCACCAG ACAGAGGGAG ACGGAGACGT TTTAGCATTT
    CTTACTGGCC AGGAAGAGGT AGAAACTGTT GTGTCGATGC TCATCGAGCA GGCTCGAGCA
    CTAGCTCGCA CTGGGATGAA GAGACACCTC CGAGTTCTCC CCATGTATGC AGGACTGCCT
    TCCTTTGAGC AAATGAAAGT GTTTGAAAGG GTGTCACGCA GTGTCAGAAA GGTGATAGTG
    GCCACCAATG TGGCAGAAAC CTCTATCACA ATCAGCGGCA TTGTGTATGT GATCGACTGT
    GGCTTTGTGA AACTCCGAGC CTACAATCCC AGGACAGCTA TTGAATGCTT GGTGGTGGTG
    CCAGTCTCCC AGGCATCAGC TAATCAGCGA GCAGGACGTG GTGGTCGTAG TCGCTCGGGA
    AAATGTTATC GCCTTTATAC AGAGGAAGCC TTTGACAAGT TGCCTCAGTC TACGGTTCCT
    GAGATGCAGC GTAGTAATTT GGCACCTGTC ATCCTGCAGC TGAAAGCACT AGGAATTGAC
    AATGTCCTCA GGTTCCACTT CATGTCGCCC CCTCCAGCAC AGTCGATGGT TCAAGCCTTG
    GAGTTACTGT ATGCTCTGGG AGGTCTGGAC AAAGACTGTC GCCTAACTGA ACCGCTTGGC
    ATGAGAATTG CAGAGTTTCC TTTGAATCCC ATGTTTGCCA AAATGCTGCT TGAATCAGGA
    AACTTCGGCT GTTCTCAGGA AATTCTAAGC ATCGCTGCCA TGATGCAGAT CCAGAATATC
    TTTGTGGTCC CCCCAAACCA GAAGTCTCAC GCACACAATA AGGACTCTAA ATGGTGTCAG
    GAACATTTCC TGAATTACAA GGGTCTTGTC AGAGCTGCGA CTGTAAGAGA ACAATTGAAA
    AAGCTTCTTG TCAAGTTTCA AGTGCCCAGG AAGTCTAGTG AAGGTGACCC GGATCTGGTT
    CTGAGGTGCA TTGTCTCCGG CTTCTTCGCC AATGCAGCGA GGTTTCATTC TACTGGAGCT
    TATAGGACCA TCCGTGATGA CCATGAGCTG CACATACACC CTGCGTCAGT CCTCTATGCA
    GAGAAGCCGC CTCGCTGGGT CATCTATAAC GAAGTTATAC AGACCTCCAA GTACTACATG
    AGAGATGTGA CTGCCATTGA ATCGGCCTGG CTGTTGGAGC TGGCTCCACA CTTTTATCAA
    CAAGGAACGC ACCTGTCTCT GAAAGCCAAA AGGGCCAAGG TCCAGGACCC GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011527274.1
    CDS31..2064
    Translation

    Target ORF information:

    RefSeq Version XM_011528972.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DEAH-box helicase 35 (DHX35), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011528972.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGCTGCGC CCGTGGGACC GGTGAAGTTC TGGCGACCCG GTACAGAGGG GCCAGGTGTA 
    AGCATCTCTG AAGAGAGACA AAGTCTGGCT GAAAACTCTG GGACAACGGT TGTTTACAAC
    CCTTATGCTG CCCTTTCCAT AGAGCAGCAG AGGCAGAAGC TGCCGGTATT CAAGCTTAGG
    AATCATATTT TATACTTGAT AGAAAATTAT CAGACAGTGG TGATTGTTGG TGAAACAGGA
    TGTGGGAAGA GCACACAGAT TCCTCAGTAC CTTGCAGAAG CCGGCTGGAC AGCTGAAGGA
    AGAGTGGTAG GAGTGACCCA GCCTCGAAGA GTGGCTGCTG TTACAGTTGC AGGGAGAGTA
    GCTGAAGAAA GGGGTGCAGT GCTGGGCCAC GAGGTGGGCT ACTGCATCCG CTTTGATGAC
    TGCACCGACC AGCTGGCCAC GAGAATTAAG TTTCTTACTG ATGGAATGCT GGTCAGGGAA
    ATGATGGTTG ATCCGTTGTT AACAAAATAT AGTGTCATCA TGCTGGATGA AGCCCACGAG
    AGGACCTTGT ACACTGACAT TGCCATTGGC TTGCTAAAAA AGATTCAGAA AAAGCGAGGG
    GATCTTCGAT TGATTGTAGC TTCAGCCACT CTGGATGCAG ACAAATTCCG GGATTTCTTT
    AATCAAAATG AAACCAGTGA TCCAGCAAGG GATACATGTG TGATCCTTAC AGTGGAAGGG
    AGAACATTTC CGGTGGATAT CTTTTATCTA CAAAGTCCTG TTCCAGATTA TATCAAATCA
    ACTGTCGAAA CTGTGGTGAA AATTCACCAG ACAGAGGGAG ACGGAGACGT TTTAGCATTT
    CTTACTGGCC AGGAAGAGGT AGAAACTGTT GTGTCGATGC TCATCGAGCA GGCTCGAGCA
    CTAGCTCGCA CTGGGATGAA GAGACACCTC CGAGTTCTCC CCATGTATGC AGGACTGCCT
    TCCTTTGAGC AAATGAAAGT GTTTGAAAGG GTGTCACGCA GTGTCAGAAA GGTGATAGTG
    GCCACCAATG TGGCAGAAAC CTCTATCACA ATCAGCGGCA TTGTGTATGT GATCGACTGT
    GGCTTTGTGA AACTCCGAGC CTACAATCCC AGGACAGCTA TTGAATGCTT GGTGGTGGTG
    CCAGTCTCCC AGGCATCAGC TAATCAGCGA GCAGGACGTG GTGGTCGTAG TCGCTCGGGA
    AAATGTTATC GCCTTTATAC AGAGGAAGCC TTTGACAAGT TGCCTCAGTC TACGGTTCCT
    GAGATGCAGC GTAGTAATTT GGCACCTGTC ATCCTGCAGC TGAAAGCACT AGGAATTGAC
    AATGTCCTCA GGTTCCACTT CATGTCGCCC CCTCCAGCAC AGTCGATGGT TCAAGCCTTG
    GAGTTACTGT ATGCTCTGGG AGGTCTGGAC AAAGACTGTC GCCTAACTGA ACCGCTTGGC
    ATGAGAATTG CAGAGTTTCC TTTGAATCCC ATGTTTGCCA AAATGCTGCT TGAATCAGGA
    AACTTCGGCT GTTCTCAGGA AATTCTAAGC ATCGCTGCCA TGATGCAGAT CCAGAATATC
    TTTGTGGTCC CCCCAAACCA GAAGTCTCAC GCACACAATA AGGACTCTAA ATGGTGTCAG
    GAACATTTCC TGAATTACAA GGGTCTTGTC AGAGCTGCGA CTGTAAGAGA ACAATTGAAA
    AAGCTTCTTG TCAAGTTTCA AGTGCCCAGG AAGTCTAGTG AAGGTGACCC GGATCTGGTT
    CTGAGGTGCA TTGTCTCCGG CTTCTTCGCC AATGCAGCGA GGTTTCATTC TACTGGAGCT
    TATAGGACCA TCCGTGATGA CCATGAGCTG CACATACACC CTGCGTCAGT CCTCTATGCA
    GAGAAGCCGC CTCGCTGGGT CATCTATAAC GAAGTTATAC AGACCTCCAA GTACTACATG
    AGAGATGTGA CTGCCATTGA ATCGGCCTGG CTGTTGGAGC TGGCTCCACA CTTTTATCAA
    CAAGGAACGC ACCTGTCTCT GAAAGCCAAA AGGGCCAAGG TCCAGGACCC GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71095
    Clone ID Related Accession (Same CDS sequence) XM_011528972.1 , XM_011528972.2
    Accession Version XM_011528972.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2034bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product probable ATP-dependent RNA helicase DHX35 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011528972.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGCTGCGC CCGTGGGACC GGTGAAGTTC TGGCGACCCG GTACAGAGGG GCCAGGTGTA 
    AGCATCTCTG AAGAGAGACA AAGTCTGGCT GAAAACTCTG GGACAACGGT TGTTTACAAC
    CCTTATGCTG CCCTTTCCAT AGAGCAGCAG AGGCAGAAGC TGCCGGTATT CAAGCTTAGG
    AATCATATTT TATACTTGAT AGAAAATTAT CAGACAGTGG TGATTGTTGG TGAAACAGGA
    TGTGGGAAGA GCACACAGAT TCCTCAGTAC CTTGCAGAAG CCGGCTGGAC AGCTGAAGGA
    AGAGTGGTAG GAGTGACCCA GCCTCGAAGA GTGGCTGCTG TTACAGTTGC AGGGAGAGTA
    GCTGAAGAAA GGGGTGCAGT GCTGGGCCAC GAGGTGGGCT ACTGCATCCG CTTTGATGAC
    TGCACCGACC AGCTGGCCAC GAGAATTAAG TTTCTTACTG ATGGAATGCT GGTCAGGGAA
    ATGATGGTTG ATCCGTTGTT AACAAAATAT AGTGTCATCA TGCTGGATGA AGCCCACGAG
    AGGACCTTGT ACACTGACAT TGCCATTGGC TTGCTAAAAA AGATTCAGAA AAAGCGAGGG
    GATCTTCGAT TGATTGTAGC TTCAGCCACT CTGGATGCAG ACAAATTCCG GGATTTCTTT
    AATCAAAATG AAACCAGTGA TCCAGCAAGG GATACATGTG TGATCCTTAC AGTGGAAGGG
    AGAACATTTC CGGTGGATAT CTTTTATCTA CAAAGTCCTG TTCCAGATTA TATCAAATCA
    ACTGTCGAAA CTGTGGTGAA AATTCACCAG ACAGAGGGAG ACGGAGACGT TTTAGCATTT
    CTTACTGGCC AGGAAGAGGT AGAAACTGTT GTGTCGATGC TCATCGAGCA GGCTCGAGCA
    CTAGCTCGCA CTGGGATGAA GAGACACCTC CGAGTTCTCC CCATGTATGC AGGACTGCCT
    TCCTTTGAGC AAATGAAAGT GTTTGAAAGG GTGTCACGCA GTGTCAGAAA GGTGATAGTG
    GCCACCAATG TGGCAGAAAC CTCTATCACA ATCAGCGGCA TTGTGTATGT GATCGACTGT
    GGCTTTGTGA AACTCCGAGC CTACAATCCC AGGACAGCTA TTGAATGCTT GGTGGTGGTG
    CCAGTCTCCC AGGCATCAGC TAATCAGCGA GCAGGACGTG GTGGTCGTAG TCGCTCGGGA
    AAATGTTATC GCCTTTATAC AGAGGAAGCC TTTGACAAGT TGCCTCAGTC TACGGTTCCT
    GAGATGCAGC GTAGTAATTT GGCACCTGTC ATCCTGCAGC TGAAAGCACT AGGAATTGAC
    AATGTCCTCA GGTTCCACTT CATGTCGCCC CCTCCAGCAC AGTCGATGGT TCAAGCCTTG
    GAGTTACTGT ATGCTCTGGG AGGTCTGGAC AAAGACTGTC GCCTAACTGA ACCGCTTGGC
    ATGAGAATTG CAGAGTTTCC TTTGAATCCC ATGTTTGCCA AAATGCTGCT TGAATCAGGA
    AACTTCGGCT GTTCTCAGGA AATTCTAAGC ATCGCTGCCA TGATGCAGAT CCAGAATATC
    TTTGTGGTCC CCCCAAACCA GAAGTCTCAC GCACACAATA AGGACTCTAA ATGGTGTCAG
    GAACATTTCC TGAATTACAA GGGTCTTGTC AGAGCTGCGA CTGTAAGAGA ACAATTGAAA
    AAGCTTCTTG TCAAGTTTCA AGTGCCCAGG AAGTCTAGTG AAGGTGACCC GGATCTGGTT
    CTGAGGTGCA TTGTCTCCGG CTTCTTCGCC AATGCAGCGA GGTTTCATTC TACTGGAGCT
    TATAGGACCA TCCGTGATGA CCATGAGCTG CACATACACC CTGCGTCAGT CCTCTATGCA
    GAGAAGCCGC CTCGCTGGGT CATCTATAAC GAAGTTATAC AGACCTCCAA GTACTACATG
    AGAGATGTGA CTGCCATTGA ATCGGCCTGG CTGTTGGAGC TGGCTCCACA CTTTTATCAA
    CAAGGAACGC ACCTGTCTCT GAAAGCCAAA AGGGCCAAGG TCCAGGACCC GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011527274.1
    CDS25..2058
    Translation

    Target ORF information:

    RefSeq Version XM_011528972.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DEAH-box helicase 35 (DHX35), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011528972.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGCTGCGC CCGTGGGACC GGTGAAGTTC TGGCGACCCG GTACAGAGGG GCCAGGTGTA 
    AGCATCTCTG AAGAGAGACA AAGTCTGGCT GAAAACTCTG GGACAACGGT TGTTTACAAC
    CCTTATGCTG CCCTTTCCAT AGAGCAGCAG AGGCAGAAGC TGCCGGTATT CAAGCTTAGG
    AATCATATTT TATACTTGAT AGAAAATTAT CAGACAGTGG TGATTGTTGG TGAAACAGGA
    TGTGGGAAGA GCACACAGAT TCCTCAGTAC CTTGCAGAAG CCGGCTGGAC AGCTGAAGGA
    AGAGTGGTAG GAGTGACCCA GCCTCGAAGA GTGGCTGCTG TTACAGTTGC AGGGAGAGTA
    GCTGAAGAAA GGGGTGCAGT GCTGGGCCAC GAGGTGGGCT ACTGCATCCG CTTTGATGAC
    TGCACCGACC AGCTGGCCAC GAGAATTAAG TTTCTTACTG ATGGAATGCT GGTCAGGGAA
    ATGATGGTTG ATCCGTTGTT AACAAAATAT AGTGTCATCA TGCTGGATGA AGCCCACGAG
    AGGACCTTGT ACACTGACAT TGCCATTGGC TTGCTAAAAA AGATTCAGAA AAAGCGAGGG
    GATCTTCGAT TGATTGTAGC TTCAGCCACT CTGGATGCAG ACAAATTCCG GGATTTCTTT
    AATCAAAATG AAACCAGTGA TCCAGCAAGG GATACATGTG TGATCCTTAC AGTGGAAGGG
    AGAACATTTC CGGTGGATAT CTTTTATCTA CAAAGTCCTG TTCCAGATTA TATCAAATCA
    ACTGTCGAAA CTGTGGTGAA AATTCACCAG ACAGAGGGAG ACGGAGACGT TTTAGCATTT
    CTTACTGGCC AGGAAGAGGT AGAAACTGTT GTGTCGATGC TCATCGAGCA GGCTCGAGCA
    CTAGCTCGCA CTGGGATGAA GAGACACCTC CGAGTTCTCC CCATGTATGC AGGACTGCCT
    TCCTTTGAGC AAATGAAAGT GTTTGAAAGG GTGTCACGCA GTGTCAGAAA GGTGATAGTG
    GCCACCAATG TGGCAGAAAC CTCTATCACA ATCAGCGGCA TTGTGTATGT GATCGACTGT
    GGCTTTGTGA AACTCCGAGC CTACAATCCC AGGACAGCTA TTGAATGCTT GGTGGTGGTG
    CCAGTCTCCC AGGCATCAGC TAATCAGCGA GCAGGACGTG GTGGTCGTAG TCGCTCGGGA
    AAATGTTATC GCCTTTATAC AGAGGAAGCC TTTGACAAGT TGCCTCAGTC TACGGTTCCT
    GAGATGCAGC GTAGTAATTT GGCACCTGTC ATCCTGCAGC TGAAAGCACT AGGAATTGAC
    AATGTCCTCA GGTTCCACTT CATGTCGCCC CCTCCAGCAC AGTCGATGGT TCAAGCCTTG
    GAGTTACTGT ATGCTCTGGG AGGTCTGGAC AAAGACTGTC GCCTAACTGA ACCGCTTGGC
    ATGAGAATTG CAGAGTTTCC TTTGAATCCC ATGTTTGCCA AAATGCTGCT TGAATCAGGA
    AACTTCGGCT GTTCTCAGGA AATTCTAAGC ATCGCTGCCA TGATGCAGAT CCAGAATATC
    TTTGTGGTCC CCCCAAACCA GAAGTCTCAC GCACACAATA AGGACTCTAA ATGGTGTCAG
    GAACATTTCC TGAATTACAA GGGTCTTGTC AGAGCTGCGA CTGTAAGAGA ACAATTGAAA
    AAGCTTCTTG TCAAGTTTCA AGTGCCCAGG AAGTCTAGTG AAGGTGACCC GGATCTGGTT
    CTGAGGTGCA TTGTCTCCGG CTTCTTCGCC AATGCAGCGA GGTTTCATTC TACTGGAGCT
    TATAGGACCA TCCGTGATGA CCATGAGCTG CACATACACC CTGCGTCAGT CCTCTATGCA
    GAGAAGCCGC CTCGCTGGGT CATCTATAAC GAAGTTATAC AGACCTCCAA GTACTACATG
    AGAGATGTGA CTGCCATTGA ATCGGCCTGG CTGTTGGAGC TGGCTCCACA CTTTTATCAA
    CAAGGAACGC ACCTGTCTCT GAAAGCCAAA AGGGCCAAGG TCCAGGACCC GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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