ROBO2 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following ROBO2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ROBO2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu22766 NM_001128929.3
Latest version!
Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$659.50-$923.30
$1319.00
OHu12688 NM_001290065.2
Latest version!
Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu94465 XM_017006991.1
Latest version!
Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu94466 XM_017006992.1
Latest version!
Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu94467 XM_017006993.1
Latest version!
Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu94468 XM_017006994.1
Latest version!
Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu94469 XM_017006995.1
Latest version!
Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu94470 XM_017006996.1
Latest version!
Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu94471 XM_017006997.1
Latest version!
Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu94472 XM_017006998.1
Latest version!
Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu94473 XM_017006999.1
Latest version!
Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu94474 XM_017007000.1
Latest version!
Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu94475 XM_017007001.1
Latest version!
Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu94476 XM_017007002.1
Latest version!
Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu94477 XM_017007003.1
Latest version!
Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu94478 XM_017007004.1
Latest version!
Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu94479 XM_017007005.1
Latest version!
Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu94480 XM_017007006.1
Latest version!
Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X26, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu94460 XM_017006986.1
Latest version!
Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OHu94461 XM_017006987.1
Latest version!
Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OHu94462 XM_017006988.1
Latest version!
Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OHu94463 XM_017006989.1
Latest version!
Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OHu94464 XM_017006990.1
Latest version!
Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OHu11403 NM_002942.5
Latest version!
Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $923.30
$1319.00
OHu12785 NM_001290040.2
Latest version!
Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu12905 NM_001290039.2
Latest version!
Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu60280 XM_011533981.2
Latest version!
Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu60281 XM_011533982.1
Latest version!
Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu60282 XM_011533983.1
Latest version!
Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu60283 XM_011533984.1
Latest version!
Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu60284 XM_011533985.1
Latest version!
Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
View Old Accession Versions >>
OHu11403 NM_002942.4 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $923.30
$1319.00
OHu12905 NM_001290039.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu12785 NM_001290040.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu12688 NM_001290065.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu22766
    Clone ID Related Accession (Same CDS sequence) NM_001128929.3
    Accession Version NM_001128929.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4185bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-22
    Organism Homo sapiens(human)
    Product roundabout homolog 2 isoform ROBO2a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC126467.6, DQ533873.1 and AC133040.3. This sequence is a reference standard in the RefSeqGene project. On Feb 22, 2014 this sequence version replaced NM_001128929.2. Transcript Variant: This variant (1) represents the use of an alternate promoter and has multiple differences compared to variant 4. These differences result in a distinct 5' UTR and the use of an alternate start codon compared to variant 4. The encoded protein (isoform ROBO2a; PMID 16829019) has a distinct N-terminus and is shorter than isoform 4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ533873.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154665, SAMEA2157437 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCCAGAA GACATGAACG TGTCACTAGA AGGATGTGGA CATGGGCTCC GGGACTGTTG 
    ATGATGACTG TGGTGTTTTG GGGTCATCAG GGGAATGGAC AAGGCCAAGG ATCGCGTCTT
    CGCCAGGAGG ACTTTCCCCC GCGGATTGTG GAGCATCCTT CCGATGTCAT CGTCTCTAAG
    GGCGAGCCCA CGACTCTGAA CTGCAAGGCG GAGGGCCGGC CAACGCCCAC CATTGAGTGG
    TACAAAGATG GGGAGCGAGT GGAGACTGAC AAGGACGATC CCCGGTCCCA CAGGATGCTT
    CTGCCCAGCG GATCCTTATT CTTCTTGCGC ATCGTGCACG GGCGCAGGAG TAAACCTGAT
    GAAGGAAGCT ACGTTTGTGT TGCGAGGAAC TATCTTGGTG AAGCAGTGAG TCGAAATGCG
    TCTCTGGAAG TGGCATTGTT ACGAGATGAC TTCCGACAAA ACCCCACAGA TGTTGTAGTG
    GCAGCTGGAG AGCCTGCAAT CCTGGAGTGC CAGCCTCCCC GGGGACACCC AGAACCCACC
    ATCTACTGGA AAAAAGACAA AGTTCGAATT GATGACAAGG AAGAAAGAAT AAGTATCCGT
    GGTGGAAAAC TGATGATCTC CAATACCAGG AAAAGTGATG CAGGGATGTA TACTTGTGTT
    GGTACCAATA TGGTGGGAGA AAGGGACAGT GACCCAGCAG AGCTGACTGT CTTTGAACGA
    CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTACTGG AGGAAGAAGC TGTAGAATTT
    CGTTGTCAAG TCCAAGGAGA TCCTCAACCA ACTGTGAGGT GGAAAAAGGA TGATGCAGAC
    TTGCCAAGAG GAAGGTATGA CATCAAAGAC GATTACACAC TAAGAATTAA AAAGACCATG
    AGTACAGATG AAGGCACCTA TATGTGTATT GCTGAGAATC GGGTTGGAAA AATGGAAGCC
    TCTGCTACAC TCACCGTCCG AGCTCCCCCA CAGTTTGTGG TTCGGCCAAG AGATCAGATT
    GTTGCTCAAG GTCGAACAGT GACATTTCCC TGTGAAACTA AAGGAAACCC ACAGCCAGCT
    GTTTTTTGGC AGAAAGAAGG CAGCCAGAAC CTACTTTTCC CAAACCAACC CCAGCAGCCC
    AACAGTAGAT GCTCAGTGTC ACCAACTGGA GACCTCACAA TCACCAACAT TCAACGTTCC
    GACGCGGGTT ACTACATCTG CCAGGCTTTA ACTGTGGCAG GAAGCATTTT AGCAAAAGCT
    CAACTGGAGG TTACTGATGT TTTGACAGAT AGACCTCCAC CTATAATTCT ACAAGGCCCA
    GCCAACCAAA CGCTGGCAGT GGATGGTACA GCGTTACTGA AATGTAAAGC CACTGGTGAT
    CCTCTTCCTG TAATTAGCTG GTTAAAGGAG GGATTTACTT TTCCGGGTAG AGATCCAAGA
    GCAACAATTC AAGAGCAAGG CACACTGCAG ATTAAGAATT TACGGATTTC TGATACTGGC
    ACTTATACTT GTGTGGCTAC AAGTTCAAGT GGAGAGACTT CCTGGAGTGC AGTGCTGGAT
    GTGACAGAGT CTGGAGCAAC AATCAGTAAA AACTATGATT TAAGTGACCT GCCAGGGCCA
    CCATCCAAAC CGCAGGTCAC TGATGTTACT AAGAACAGTG TCACCTTGTC CTGGCAGCCA
    GGTACCCCTG GAACCCTTCC AGCAAGTGCA TATATCATTG AGGCTTTCAG CCAATCAGTG
    AGCAACAGCT GGCAGACCGT GGCAAACCAT GTAAAGACCA CCCTCTATAC TGTAAGAGGA
    CTGCGGCCCA ATACAATCTA CTTATTCATG GTCAGAGCGA TCAACCCCCA AGGTCTCAGT
    GACCCAAGTC CCATGTCAGA TCCTGTGCGC ACACAAGATA TCAGCCCACC AGCACAAGGA
    GTGGACCACA GGCAAGTGCA GAAAGAGCTA GGAGATGTCC TTGTCCGTCT TCATAATCCA
    GTTGTGCTGA CTCCCACCAC GGTTCAGGTC ACATGGACGG TTGATCGCCA ACCCCAGTTT
    ATCCAAGGCT ACCGAGTGAT GTATCGTCAG ACTTCAGGTC TGCAGGCGAC ATCTTCGTGG
    CAGAATTTAG ATGCCAAAGT CCCGACTGAA CGAAGTGCTG TCTTAGTCAA CCTGAAAAAG
    GGGGTGACTT ATGAAATTAA AGTACGGCCA TATTTTAATG AGTTCCAAGG AATGGATAGT
    GAATCTAAAA CGGTTCGTAC TACTGAAGAA GCCCCAAGTG CCCCACCACA GTCTGTCACT
    GTACTGACAG TTGGAAGCTA CAATAGCACA AGTATTAGTG TTTCCTGGGA TCCTCCTCCT
    CCAGATCACC AGAATGGAAT TATCCAAGAA TACAAGATCT GGTGTCTAGG AAATGAAACG
    CGATTCCATA TCAACAAAAC TGTGGATGCA GCCATTCGGT CCGTAATAAT TGGTGGATTA
    TTCCCAGGTA TTCAATACCG GGTAGAGGTT GCAGCTAGTA CCAGTGCAGG GGTTGGAGTA
    AAGAGTGAGC CACAGCCAAT AATAATCGGG AGACGCAATG AAGTTGTCAT TACTGAAAAC
    AATAACAGCA TAACTGAGCA AATCACTGAT GTGGTGAAGC AACCAGCCTT TATAGCTGGT
    ATTGGTGGTG CCTGCTGGGT AATTCTGATG GGTTTTAGCA TATGGTTGTA TTGGCGAAGA
    AAGAAGAGGA AGGGACTCAG TAATTATGCT GTTACGTTTC AAAGAGGAGA TGGAGGACTA
    ATGAGCAATG GAAGCCGTCC AGGTCTTCTC AATGCTGGTG ATCCCAGCTA TCCATGGCTT
    GCTGATTCTT GGCCAGCCAC GAGCTTGCCA GTAAATAATA GCAACAGTGG CCCAAATGAG
    ATTGGAAATT TTGGCCGTGG AGATGTGCTG CCACCAGTTC CAGGCCAAGG GGATAAAACA
    GCAACGATGC TCTCAGATGG AGCCATTTAT AGTAGCATTG ACTTCACTAC CAAAACCAGT
    TACAACAGTT CCAGCCAAAT AACACAGGCT ACCCCATATG CCACGACACA GATCTTGCAT
    TCCAACAGCA TACATGAATT GGCTGTCGAT CTGCCTGATC CACAATGGAA AAGCTCAATT
    CAGCAAAAAA CAGATCTGAT GGGATTTGGT TATTCTCTAC CTGATCAGAA CAAAGGTAAC
    AATGGTGGGA AAGGTGGAAA AAAGAAGAAA AATAAAAACT CTTCTAAACC ACAGAAAAAC
    AATGGATCCA CTTGGGCCAA TGTCCCTCTA CCTCCCCCCC CAGTCCAGCC CCTTCCTGGC
    ACGGAGCTGG AACACTATGC AGTGGAACAA CAAGAAAATG GCTATGACAG TGATAGCTGG
    TGCCCACCAT TGCCAGTACA AACTTACTTA CACCAAGGTC TGGAAGATGA ACTGGAAGAA
    GATGATGATA GGGTCCCAAC ACCTCCTGTT CGAGGCGTGG CTTCTTCTCC TGCTATCTCC
    TTTGGACAGC AGTCCACTGC AACTCTTACT CCATCCCCAC GGGAAGAGAT GCAACCCATG
    CTGCAGGCTC ACCTGGATGA GTTGACAAGA GCCTATCAGT TTGATATAGC AAAACAAACA
    TGGCACATTC AAAGCAATAA TCAACCTCCA CAGCCTCCAG TTCCACCGTT AGGTTATGTG
    TCTGGAGCCT TGATTTCTGA TTTGGAAACG GATGTTGCAG ATGATGATGC CGACGACGAA
    GAGGAAGCTT TAGAAATCCC CAGGCCCCTG AGAGCACTGG ACCAGACTCC TGGATCCAGC
    ATGGACAATC TAGACAGCTC TGTGACAGGA AAAGCCTTTA CCTCCTCTCA AAGACCTCGA
    CCTACCAGCC CATTTTCTAC TGACAGTAAC ACCAGTGCAG CCCTGAGTCA AAGTCAGAGG
    CCTCGGCCCA CTAAAAAACA CAAGGGAGGG CGGATGGACC AACAACCAGC ATTGCCTCAT
    CGAAGGGAAG GAATGACAGA TGAGGAGGCC TTGGTGCCCT ATAGCAAGCC CAGTTTCCCA
    TCTCCAGGTG GCCACAGCTC ATCAGGAACA GCTTCTTCTA AGGGATCCAC TGGACCTAGG
    AAAACCGAGG TGTTGAGAGC AGGCCACCAG CGCAATGCCA GCGACCTTCT TGACATAGGA
    TATATGGGCT CCAACAGTCA AGGACAGTTT ACAGGTGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001122401.1
    CDS300..4484
    Translation

    Target ORF information:

    RefSeq Version NM_001128929.3
    Organism Homo sapiens(human)
    Definition Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001128929.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCCAGAA GACATGAACG TGTCACTAGA AGGATGTGGA CATGGGCTCC GGGACTGTTG 
    ATGATGACTG TGGTGTTTTG GGGTCATCAG GGGAATGGAC AAGGCCAAGG ATCGCGTCTT
    CGCCAGGAGG ACTTTCCCCC GCGGATTGTG GAGCATCCTT CCGATGTCAT CGTCTCTAAG
    GGCGAGCCCA CGACTCTGAA CTGCAAGGCG GAGGGCCGGC CAACGCCCAC CATTGAGTGG
    TACAAAGATG GGGAGCGAGT GGAGACTGAC AAGGACGATC CCCGGTCCCA CAGGATGCTT
    CTGCCCAGCG GATCCTTATT CTTCTTGCGC ATCGTGCACG GGCGCAGGAG TAAACCTGAT
    GAAGGAAGCT ACGTTTGTGT TGCGAGGAAC TATCTTGGTG AAGCAGTGAG TCGAAATGCG
    TCTCTGGAAG TGGCATTGTT ACGAGATGAC TTCCGACAAA ACCCCACAGA TGTTGTAGTG
    GCAGCTGGAG AGCCTGCAAT CCTGGAGTGC CAGCCTCCCC GGGGACACCC AGAACCCACC
    ATCTACTGGA AAAAAGACAA AGTTCGAATT GATGACAAGG AAGAAAGAAT AAGTATCCGT
    GGTGGAAAAC TGATGATCTC CAATACCAGG AAAAGTGATG CAGGGATGTA TACTTGTGTT
    GGTACCAATA TGGTGGGAGA AAGGGACAGT GACCCAGCAG AGCTGACTGT CTTTGAACGA
    CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTACTGG AGGAAGAAGC TGTAGAATTT
    CGTTGTCAAG TCCAAGGAGA TCCTCAACCA ACTGTGAGGT GGAAAAAGGA TGATGCAGAC
    TTGCCAAGAG GAAGGTATGA CATCAAAGAC GATTACACAC TAAGAATTAA AAAGACCATG
    AGTACAGATG AAGGCACCTA TATGTGTATT GCTGAGAATC GGGTTGGAAA AATGGAAGCC
    TCTGCTACAC TCACCGTCCG AGCTCCCCCA CAGTTTGTGG TTCGGCCAAG AGATCAGATT
    GTTGCTCAAG GTCGAACAGT GACATTTCCC TGTGAAACTA AAGGAAACCC ACAGCCAGCT
    GTTTTTTGGC AGAAAGAAGG CAGCCAGAAC CTACTTTTCC CAAACCAACC CCAGCAGCCC
    AACAGTAGAT GCTCAGTGTC ACCAACTGGA GACCTCACAA TCACCAACAT TCAACGTTCC
    GACGCGGGTT ACTACATCTG CCAGGCTTTA ACTGTGGCAG GAAGCATTTT AGCAAAAGCT
    CAACTGGAGG TTACTGATGT TTTGACAGAT AGACCTCCAC CTATAATTCT ACAAGGCCCA
    GCCAACCAAA CGCTGGCAGT GGATGGTACA GCGTTACTGA AATGTAAAGC CACTGGTGAT
    CCTCTTCCTG TAATTAGCTG GTTAAAGGAG GGATTTACTT TTCCGGGTAG AGATCCAAGA
    GCAACAATTC AAGAGCAAGG CACACTGCAG ATTAAGAATT TACGGATTTC TGATACTGGC
    ACTTATACTT GTGTGGCTAC AAGTTCAAGT GGAGAGACTT CCTGGAGTGC AGTGCTGGAT
    GTGACAGAGT CTGGAGCAAC AATCAGTAAA AACTATGATT TAAGTGACCT GCCAGGGCCA
    CCATCCAAAC CGCAGGTCAC TGATGTTACT AAGAACAGTG TCACCTTGTC CTGGCAGCCA
    GGTACCCCTG GAACCCTTCC AGCAAGTGCA TATATCATTG AGGCTTTCAG CCAATCAGTG
    AGCAACAGCT GGCAGACCGT GGCAAACCAT GTAAAGACCA CCCTCTATAC TGTAAGAGGA
    CTGCGGCCCA ATACAATCTA CTTATTCATG GTCAGAGCGA TCAACCCCCA AGGTCTCAGT
    GACCCAAGTC CCATGTCAGA TCCTGTGCGC ACACAAGATA TCAGCCCACC AGCACAAGGA
    GTGGACCACA GGCAAGTGCA GAAAGAGCTA GGAGATGTCC TTGTCCGTCT TCATAATCCA
    GTTGTGCTGA CTCCCACCAC GGTTCAGGTC ACATGGACGG TTGATCGCCA ACCCCAGTTT
    ATCCAAGGCT ACCGAGTGAT GTATCGTCAG ACTTCAGGTC TGCAGGCGAC ATCTTCGTGG
    CAGAATTTAG ATGCCAAAGT CCCGACTGAA CGAAGTGCTG TCTTAGTCAA CCTGAAAAAG
    GGGGTGACTT ATGAAATTAA AGTACGGCCA TATTTTAATG AGTTCCAAGG AATGGATAGT
    GAATCTAAAA CGGTTCGTAC TACTGAAGAA GCCCCAAGTG CCCCACCACA GTCTGTCACT
    GTACTGACAG TTGGAAGCTA CAATAGCACA AGTATTAGTG TTTCCTGGGA TCCTCCTCCT
    CCAGATCACC AGAATGGAAT TATCCAAGAA TACAAGATCT GGTGTCTAGG AAATGAAACG
    CGATTCCATA TCAACAAAAC TGTGGATGCA GCCATTCGGT CCGTAATAAT TGGTGGATTA
    TTCCCAGGTA TTCAATACCG GGTAGAGGTT GCAGCTAGTA CCAGTGCAGG GGTTGGAGTA
    AAGAGTGAGC CACAGCCAAT AATAATCGGG AGACGCAATG AAGTTGTCAT TACTGAAAAC
    AATAACAGCA TAACTGAGCA AATCACTGAT GTGGTGAAGC AACCAGCCTT TATAGCTGGT
    ATTGGTGGTG CCTGCTGGGT AATTCTGATG GGTTTTAGCA TATGGTTGTA TTGGCGAAGA
    AAGAAGAGGA AGGGACTCAG TAATTATGCT GTTACGTTTC AAAGAGGAGA TGGAGGACTA
    ATGAGCAATG GAAGCCGTCC AGGTCTTCTC AATGCTGGTG ATCCCAGCTA TCCATGGCTT
    GCTGATTCTT GGCCAGCCAC GAGCTTGCCA GTAAATAATA GCAACAGTGG CCCAAATGAG
    ATTGGAAATT TTGGCCGTGG AGATGTGCTG CCACCAGTTC CAGGCCAAGG GGATAAAACA
    GCAACGATGC TCTCAGATGG AGCCATTTAT AGTAGCATTG ACTTCACTAC CAAAACCAGT
    TACAACAGTT CCAGCCAAAT AACACAGGCT ACCCCATATG CCACGACACA GATCTTGCAT
    TCCAACAGCA TACATGAATT GGCTGTCGAT CTGCCTGATC CACAATGGAA AAGCTCAATT
    CAGCAAAAAA CAGATCTGAT GGGATTTGGT TATTCTCTAC CTGATCAGAA CAAAGGTAAC
    AATGGTGGGA AAGGTGGAAA AAAGAAGAAA AATAAAAACT CTTCTAAACC ACAGAAAAAC
    AATGGATCCA CTTGGGCCAA TGTCCCTCTA CCTCCCCCCC CAGTCCAGCC CCTTCCTGGC
    ACGGAGCTGG AACACTATGC AGTGGAACAA CAAGAAAATG GCTATGACAG TGATAGCTGG
    TGCCCACCAT TGCCAGTACA AACTTACTTA CACCAAGGTC TGGAAGATGA ACTGGAAGAA
    GATGATGATA GGGTCCCAAC ACCTCCTGTT CGAGGCGTGG CTTCTTCTCC TGCTATCTCC
    TTTGGACAGC AGTCCACTGC AACTCTTACT CCATCCCCAC GGGAAGAGAT GCAACCCATG
    CTGCAGGCTC ACCTGGATGA GTTGACAAGA GCCTATCAGT TTGATATAGC AAAACAAACA
    TGGCACATTC AAAGCAATAA TCAACCTCCA CAGCCTCCAG TTCCACCGTT AGGTTATGTG
    TCTGGAGCCT TGATTTCTGA TTTGGAAACG GATGTTGCAG ATGATGATGC CGACGACGAA
    GAGGAAGCTT TAGAAATCCC CAGGCCCCTG AGAGCACTGG ACCAGACTCC TGGATCCAGC
    ATGGACAATC TAGACAGCTC TGTGACAGGA AAAGCCTTTA CCTCCTCTCA AAGACCTCGA
    CCTACCAGCC CATTTTCTAC TGACAGTAAC ACCAGTGCAG CCCTGAGTCA AAGTCAGAGG
    CCTCGGCCCA CTAAAAAACA CAAGGGAGGG CGGATGGACC AACAACCAGC ATTGCCTCAT
    CGAAGGGAAG GAATGACAGA TGAGGAGGCC TTGGTGCCCT ATAGCAAGCC CAGTTTCCCA
    TCTCCAGGTG GCCACAGCTC ATCAGGAACA GCTTCTTCTA AGGGATCCAC TGGACCTAGG
    AAAACCGAGG TGTTGAGAGC AGGCCACCAG CGCAATGCCA GCGACCTTCT TGACATAGGA
    TATATGGGCT CCAACAGTCA AGGACAGTTT ACAGGTGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu12688
    Clone ID Related Accession (Same CDS sequence) NM_001290065.1 , NM_001290065.2
    Accession Version NM_001290065.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2358bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-29
    Organism Homo sapiens(human)
    Product roundabout homolog 2 isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ533874.1, BC144302.1 and AC133040.3. Transcript Variant: This variant (5) has multiple differences compared to variant 4. These differences result in a distinct 5' UTR and cause translation initiation at a downstream start codon compared to variant 4; the 5'-most initiation codon, as used in variant 4, is associated with a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the downstream AUG resulting in an isoform (5) with a shorter N-terminus compared to isoform 4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC144302.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465404 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG 
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG
    CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG
    GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT
    CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT
    GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG
    CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA
    GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG
    GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC
    GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT
    GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT
    CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG
    CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC
    GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT
    GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGGTG GGAAAGGTGG AAAAAAGAAG
    AAAAATAAAA ACTCTTCTAA ACCACAGAAA AACAATGGAT CCACTTGGGC CAATGTCCCT
    CTACCTCCCC CCCCAGTCCA GCCCCTTCCT GGCACGGAGC TGGAACACTA TGCAGTGGAA
    CAACAAGAAA ATGGCTATGA CAGTGATAGC TGGTGCCCAC CATTGCCAGT ACAAACTTAC
    TTACACCAAG GTCTGGAAGA TGAACTGGAA GAAGATGATG ATAGGGTCCC AACACCTCCT
    GTTCGAGGCG TGGCTTCTTC TCCTGCTATC TCCTTTGGAC AGCAGTCCAC TGCAACTCTT
    ACTCCATCCC CACGGGAAGA GATGCAACCC ATGCTGCAGG CTCACCTGGA TGAGTTGACA
    AGAGCCTATC AGTTTGATAT AGCAAAACAA ACATGGCACA TTCAAAGCAA TAATCAACCT
    CCACAGCCTC CAGTTCCACC GTTAGGTTAT GTGTCTGGAG CCTTGATTTC TGATTTGGAA
    ACGGATGTTG CAGATGATGA TGCCGACGAC GAAGAGGAAG CTTTAGAAAT CCCCAGGCCC
    CTGAGAGCAC TGGACCAGAC TCCTGGATCC AGCATGGACA ATCTAGACAG CTCTGTGACA
    GGAAAAGCCT TTACCTCCTC TCAAAGACCT CGACCTACCA GCCCATTTTC TACTGACAGT
    AACACCAGTG CAGCCCTGAG TCAAAGTCAG AGGCCTCGGC CCACTAAAAA ACACAAGGGA
    GGGCGGATGG ACCAACAACC AGCATTGCCT CATCGAAGGG AAGGAATGAC AGATGAGGAG
    GCCTTGGTGC CCTATAGCAA GCCCAGTTTC CCATCTCCAG GTGGCCACAG CTCATCAGGA
    ACAGCTTCTT CTAAGGGATC CACTGGACCT AGGAAAACCG AGGTGTTGAG AGCAGGCCAC
    CAGCGCAATG CCAGCGACCT TCTTGACATA GGATATATGG GCTCCAACAG TCAAGGACAG
    TTTACAGGTG AATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001276994.1
    CDS2562..4919
    Translation

    Target ORF information:

    RefSeq Version NM_001290065.1
    Organism Homo sapiens(human)
    Definition Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001290065.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG 
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG
    CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG
    GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT
    CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT
    GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG
    CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA
    GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG
    GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC
    GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT
    GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT
    CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG
    CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC
    GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT
    GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGGTG GGAAAGGTGG AAAAAAGAAG
    AAAAATAAAA ACTCTTCTAA ACCACAGAAA AACAATGGAT CCACTTGGGC CAATGTCCCT
    CTACCTCCCC CCCCAGTCCA GCCCCTTCCT GGCACGGAGC TGGAACACTA TGCAGTGGAA
    CAACAAGAAA ATGGCTATGA CAGTGATAGC TGGTGCCCAC CATTGCCAGT ACAAACTTAC
    TTACACCAAG GTCTGGAAGA TGAACTGGAA GAAGATGATG ATAGGGTCCC AACACCTCCT
    GTTCGAGGCG TGGCTTCTTC TCCTGCTATC TCCTTTGGAC AGCAGTCCAC TGCAACTCTT
    ACTCCATCCC CACGGGAAGA GATGCAACCC ATGCTGCAGG CTCACCTGGA TGAGTTGACA
    AGAGCCTATC AGTTTGATAT AGCAAAACAA ACATGGCACA TTCAAAGCAA TAATCAACCT
    CCACAGCCTC CAGTTCCACC GTTAGGTTAT GTGTCTGGAG CCTTGATTTC TGATTTGGAA
    ACGGATGTTG CAGATGATGA TGCCGACGAC GAAGAGGAAG CTTTAGAAAT CCCCAGGCCC
    CTGAGAGCAC TGGACCAGAC TCCTGGATCC AGCATGGACA ATCTAGACAG CTCTGTGACA
    GGAAAAGCCT TTACCTCCTC TCAAAGACCT CGACCTACCA GCCCATTTTC TACTGACAGT
    AACACCAGTG CAGCCCTGAG TCAAAGTCAG AGGCCTCGGC CCACTAAAAA ACACAAGGGA
    GGGCGGATGG ACCAACAACC AGCATTGCCT CATCGAAGGG AAGGAATGAC AGATGAGGAG
    GCCTTGGTGC CCTATAGCAA GCCCAGTTTC CCATCTCCAG GTGGCCACAG CTCATCAGGA
    ACAGCTTCTT CTAAGGGATC CACTGGACCT AGGAAAACCG AGGTGTTGAG AGCAGGCCAC
    CAGCGCAATG CCAGCGACCT TCTTGACATA GGATATATGG GCTCCAACAG TCAAGGACAG
    TTTACAGGTG AATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu12688
    Clone ID Related Accession (Same CDS sequence) NM_001290065.1 , NM_001290065.2
    Accession Version NM_001290065.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2358bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product roundabout homolog 2 isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC117515.8, DQ533874.1, BC144302.1 and AC133040.3. On Jun 2, 2019 this sequence version replaced NM_001290065.1. Transcript Variant: This variant (5) has multiple differences compared to variant 4. These differences result in a distinct 5' UTR and cause translation initiation at a downstream start codon compared to variant 4; the 5'-most initiation codon, as used in variant 4, is associated with a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the downstream AUG resulting in an isoform (5) with a shorter N-terminus compared to isoform 4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC144302.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465404 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG 
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG
    CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG
    GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT
    CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT
    GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG
    CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA
    GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG
    GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC
    GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT
    GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT
    CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG
    CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC
    GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT
    GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGGTG GGAAAGGTGG AAAAAAGAAG
    AAAAATAAAA ACTCTTCTAA ACCACAGAAA AACAATGGAT CCACTTGGGC CAATGTCCCT
    CTACCTCCCC CCCCAGTCCA GCCCCTTCCT GGCACGGAGC TGGAACACTA TGCAGTGGAA
    CAACAAGAAA ATGGCTATGA CAGTGATAGC TGGTGCCCAC CATTGCCAGT ACAAACTTAC
    TTACACCAAG GTCTGGAAGA TGAACTGGAA GAAGATGATG ATAGGGTCCC AACACCTCCT
    GTTCGAGGCG TGGCTTCTTC TCCTGCTATC TCCTTTGGAC AGCAGTCCAC TGCAACTCTT
    ACTCCATCCC CACGGGAAGA GATGCAACCC ATGCTGCAGG CTCACCTGGA TGAGTTGACA
    AGAGCCTATC AGTTTGATAT AGCAAAACAA ACATGGCACA TTCAAAGCAA TAATCAACCT
    CCACAGCCTC CAGTTCCACC GTTAGGTTAT GTGTCTGGAG CCTTGATTTC TGATTTGGAA
    ACGGATGTTG CAGATGATGA TGCCGACGAC GAAGAGGAAG CTTTAGAAAT CCCCAGGCCC
    CTGAGAGCAC TGGACCAGAC TCCTGGATCC AGCATGGACA ATCTAGACAG CTCTGTGACA
    GGAAAAGCCT TTACCTCCTC TCAAAGACCT CGACCTACCA GCCCATTTTC TACTGACAGT
    AACACCAGTG CAGCCCTGAG TCAAAGTCAG AGGCCTCGGC CCACTAAAAA ACACAAGGGA
    GGGCGGATGG ACCAACAACC AGCATTGCCT CATCGAAGGG AAGGAATGAC AGATGAGGAG
    GCCTTGGTGC CCTATAGCAA GCCCAGTTTC CCATCTCCAG GTGGCCACAG CTCATCAGGA
    ACAGCTTCTT CTAAGGGATC CACTGGACCT AGGAAAACCG AGGTGTTGAG AGCAGGCCAC
    CAGCGCAATG CCAGCGACCT TCTTGACATA GGATATATGG GCTCCAACAG TCAAGGACAG
    TTTACAGGTG AATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001276994.1
    CDS2606..4963
    Translation

    Target ORF information:

    RefSeq Version NM_001290065.2
    Organism Homo sapiens(human)
    Definition Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001290065.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG 
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG
    CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG
    GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT
    CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT
    GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG
    CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA
    GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG
    GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC
    GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT
    GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT
    CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG
    CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC
    GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT
    GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGGTG GGAAAGGTGG AAAAAAGAAG
    AAAAATAAAA ACTCTTCTAA ACCACAGAAA AACAATGGAT CCACTTGGGC CAATGTCCCT
    CTACCTCCCC CCCCAGTCCA GCCCCTTCCT GGCACGGAGC TGGAACACTA TGCAGTGGAA
    CAACAAGAAA ATGGCTATGA CAGTGATAGC TGGTGCCCAC CATTGCCAGT ACAAACTTAC
    TTACACCAAG GTCTGGAAGA TGAACTGGAA GAAGATGATG ATAGGGTCCC AACACCTCCT
    GTTCGAGGCG TGGCTTCTTC TCCTGCTATC TCCTTTGGAC AGCAGTCCAC TGCAACTCTT
    ACTCCATCCC CACGGGAAGA GATGCAACCC ATGCTGCAGG CTCACCTGGA TGAGTTGACA
    AGAGCCTATC AGTTTGATAT AGCAAAACAA ACATGGCACA TTCAAAGCAA TAATCAACCT
    CCACAGCCTC CAGTTCCACC GTTAGGTTAT GTGTCTGGAG CCTTGATTTC TGATTTGGAA
    ACGGATGTTG CAGATGATGA TGCCGACGAC GAAGAGGAAG CTTTAGAAAT CCCCAGGCCC
    CTGAGAGCAC TGGACCAGAC TCCTGGATCC AGCATGGACA ATCTAGACAG CTCTGTGACA
    GGAAAAGCCT TTACCTCCTC TCAAAGACCT CGACCTACCA GCCCATTTTC TACTGACAGT
    AACACCAGTG CAGCCCTGAG TCAAAGTCAG AGGCCTCGGC CCACTAAAAA ACACAAGGGA
    GGGCGGATGG ACCAACAACC AGCATTGCCT CATCGAAGGG AAGGAATGAC AGATGAGGAG
    GCCTTGGTGC CCTATAGCAA GCCCAGTTTC CCATCTCCAG GTGGCCACAG CTCATCAGGA
    ACAGCTTCTT CTAAGGGATC CACTGGACCT AGGAAAACCG AGGTGTTGAG AGCAGGCCAC
    CAGCGCAATG CCAGCGACCT TCTTGACATA GGATATATGG GCTCCAACAG TCAAGGACAG
    TTTACAGGTG AATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94465
    Clone ID Related Accession (Same CDS sequence) XM_017006991.1
    Accession Version XM_017006991.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4491bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product roundabout homolog 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
    CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT
    TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT
    CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC
    GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA
    AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC
    CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG
    TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA
    CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT
    CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC
    CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT
    GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT
    ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT
    AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC
    CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC
    ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG
    CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG
    GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT
    CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT
    GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG
    CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA
    GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG
    GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC
    GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT
    GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT
    CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG
    CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC
    GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT
    GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGGTG GGAAAGGTGG AAAAAAGAAG
    AAAAATAAAA ACTCTTCTAA ACCACAGAAA AACAATGGAT CCACTTGGGC CAATGTCCCT
    CTACCTCCCC CCCCAGTCCA GCCCCTTCCT GGCACGGAGC TGGAACACTA TGCAGTGGAA
    CAACAAGAAA ATGGCTATGA CAGTGATAGC TGGTGCCCAC CATTGCCAGT ACAAACTTAC
    TTACACCAAG GTCTGGAAGA TGAACTGGAA GAAGATGATG ATAGGGTCCC AACACCTCCT
    GTTCGAGGCG TGGCTTCTTC TCCTGCTATC TCCTTTGGAC AGCAGTCCAC TGCAACTCTT
    ACTCCATCCC CACGGGAAGA GATGCAACCC ATGCTGCAGG CTCACCTGGA TGAGTTGACA
    AGAGCCTATC AGTTTGATAT AGCAAAACAA ACATGGCACA TTCAAAGCAA TAATCAACCT
    CCACAGCCTC CAGTTCCACC GTTAGGTTAT GTGTCTGGAG CCTTGATTTC TGATTTGGAA
    ACGGATGTTG CAGATGATGA TGCCGACGAC GAAGAGGAAG CTTTAGAAAT CCCCAGGCCC
    CTGAGAGCAC TGGACCAGAC TCCTGGATCC AGCATGGACA ATCTAGACAG CTCTGTGACA
    GGAAAAGCCT TTACCTCCTC TCAAAGACCT CGACCTACCA GCCCATTTTC TACTGACAGT
    AACACCAGTG CAGCCCTGAG TCAAAGTCAG AGGCCTCGGC CCACTAAAAA ACACAAGGGA
    GGGCGGATGG ACCAACAACC AGCATTGCCT CATCGAAGGG AAGGAATGAC AGATGATCTT
    CCACCACCAC CAGATCCCCC GCCAGGTCAG GGTTTAAGGC AGCAAATAGG CCCGAGCCAG
    CAGGCTGGTA ACGTGGAAAA CTCAGCAGAG AGAAAAGGAA GCTCTCTAGA GAGACAACAT
    GCATCCAGCT TAGAAGACAC AAAGAGCTCA TTGGATTGTC CAGCTAGAAC CTCCCTAGAG
    TGGCAGCGAC AAACCCAGGA ATGGATAAGC TCCACAGAAC GACAAGAAGA TATACGGAAA
    GCCCCACACA AACAAGGTGT CGGATCAGAG GAGGCCTTGG TGCCCTATAG CAAGCCCAGT
    TTCCCATCTC CAGGTGGCCA CAGCTCATCA GGAACAGCTT CTTCTAAGGG ATCCACTGGA
    CCTAGGAAAA CCGAGGTGTT GAGAGCAGGC CACCAGCGCA ATGCCAGCGA CCTTCTTGAC
    ATAGGATATA TGGGCTCCAA CAGTCAAGGA CAGTTTACAG GTGAATTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016862480.1
    CDS400..4890
    Translation

    Target ORF information:

    RefSeq Version XM_017006991.1
    Organism Homo sapiens(human)
    Definition Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017006991.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
    CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT
    TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT
    CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC
    GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA
    AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC
    CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG
    TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA
    CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT
    CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC
    CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT
    GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT
    ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT
    AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC
    CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC
    ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG
    CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG
    GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT
    CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT
    GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG
    CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA
    GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG
    GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC
    GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT
    GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT
    CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG
    CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC
    GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT
    GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGGTG GGAAAGGTGG AAAAAAGAAG
    AAAAATAAAA ACTCTTCTAA ACCACAGAAA AACAATGGAT CCACTTGGGC CAATGTCCCT
    CTACCTCCCC CCCCAGTCCA GCCCCTTCCT GGCACGGAGC TGGAACACTA TGCAGTGGAA
    CAACAAGAAA ATGGCTATGA CAGTGATAGC TGGTGCCCAC CATTGCCAGT ACAAACTTAC
    TTACACCAAG GTCTGGAAGA TGAACTGGAA GAAGATGATG ATAGGGTCCC AACACCTCCT
    GTTCGAGGCG TGGCTTCTTC TCCTGCTATC TCCTTTGGAC AGCAGTCCAC TGCAACTCTT
    ACTCCATCCC CACGGGAAGA GATGCAACCC ATGCTGCAGG CTCACCTGGA TGAGTTGACA
    AGAGCCTATC AGTTTGATAT AGCAAAACAA ACATGGCACA TTCAAAGCAA TAATCAACCT
    CCACAGCCTC CAGTTCCACC GTTAGGTTAT GTGTCTGGAG CCTTGATTTC TGATTTGGAA
    ACGGATGTTG CAGATGATGA TGCCGACGAC GAAGAGGAAG CTTTAGAAAT CCCCAGGCCC
    CTGAGAGCAC TGGACCAGAC TCCTGGATCC AGCATGGACA ATCTAGACAG CTCTGTGACA
    GGAAAAGCCT TTACCTCCTC TCAAAGACCT CGACCTACCA GCCCATTTTC TACTGACAGT
    AACACCAGTG CAGCCCTGAG TCAAAGTCAG AGGCCTCGGC CCACTAAAAA ACACAAGGGA
    GGGCGGATGG ACCAACAACC AGCATTGCCT CATCGAAGGG AAGGAATGAC AGATGATCTT
    CCACCACCAC CAGATCCCCC GCCAGGTCAG GGTTTAAGGC AGCAAATAGG CCCGAGCCAG
    CAGGCTGGTA ACGTGGAAAA CTCAGCAGAG AGAAAAGGAA GCTCTCTAGA GAGACAACAT
    GCATCCAGCT TAGAAGACAC AAAGAGCTCA TTGGATTGTC CAGCTAGAAC CTCCCTAGAG
    TGGCAGCGAC AAACCCAGGA ATGGATAAGC TCCACAGAAC GACAAGAAGA TATACGGAAA
    GCCCCACACA AACAAGGTGT CGGATCAGAG GAGGCCTTGG TGCCCTATAG CAAGCCCAGT
    TTCCCATCTC CAGGTGGCCA CAGCTCATCA GGAACAGCTT CTTCTAAGGG ATCCACTGGA
    CCTAGGAAAA CCGAGGTGTT GAGAGCAGGC CACCAGCGCA ATGCCAGCGA CCTTCTTGAC
    ATAGGATATA TGGGCTCCAA CAGTCAAGGA CAGTTTACAG GTGAATTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94466
    Clone ID Related Accession (Same CDS sequence) XM_017006992.1
    Accession Version XM_017006992.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4470bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product roundabout homolog 2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCCAGAA GACATGAACG TGTCACTAGA AGGATGTGGA CATGGGCTCC GGGACTGTTG 
    ATGATGACTG TGGTGTTTTG GGGTCATCAG GGGAATGGAC AAGGCCAAGG ATCGCGTCTT
    CGCCAGGAGG ACTTTCCCCC GCGGATTGTG GAGCATCCTT CCGATGTCAT CGTCTCTAAG
    GGCGAGCCCA CGACTCTGAA CTGCAAGGCG GAGGGCCGGC CAACGCCCAC CATTGAGTGG
    TACAAAGATG GGGAGCGAGT GGAGACTGAC AAGGACGATC CCCGGTCCCA CAGGATGCTT
    CTGCCCAGCG GATCCTTATT CTTCTTGCGC ATCGTGCACG GGCGCAGGAG TAAACCTGAT
    GAAGGAAGCT ACGTTTGTGT TGCGAGGAAC TATCTTGGTG AAGCAGTGAG TCGAAATGCG
    TCTCTGGAAG TGGCATTGTT ACGAGATGAC TTCCGACAAA ACCCCACAGA TGTTGTAGTG
    GCAGCTGGAG AGCCTGCAAT CCTGGAGTGC CAGCCTCCCC GGGGACACCC AGAACCCACC
    ATCTACTGGA AAAAAGACAA AGTTCGAATT GATGACAAGG AAGAAAGAAT AAGTATCCGT
    GGTGGAAAAC TGATGATCTC CAATACCAGG AAAAGTGATG CAGGGATGTA TACTTGTGTT
    GGTACCAATA TGGTGGGAGA AAGGGACAGT GACCCAGCAG AGCTGACTGT CTTTGAACGA
    CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTACTGG AGGAAGAAGC TGTAGAATTT
    CGTTGTCAAG TCCAAGGAGA TCCTCAACCA ACTGTGAGGT GGAAAAAGGA TGATGCAGAC
    TTGCCAAGAG GAAGGTATGA CATCAAAGAC GATTACACAC TAAGAATTAA AAAGACCATG
    AGTACAGATG AAGGCACCTA TATGTGTATT GCTGAGAATC GGGTTGGAAA AATGGAAGCC
    TCTGCTACAC TCACCGTCCG AGCTCGCCCT GTTGCTCCCC CACAGTTTGT GGTTCGGCCA
    AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC
    CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA
    CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC
    ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT
    TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT
    CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA
    GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT
    AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT
    TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT
    GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC
    CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG
    TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC
    AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT
    ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC
    CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA
    CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT
    CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC
    CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG
    ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC
    AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA
    GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA
    CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG
    GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA
    GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA
    ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA
    GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC
    ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC
    TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG
    TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA
    GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC
    TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT
    GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA
    GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT
    ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA
    CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG
    AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG
    AACAAAGGTA ACAATGGTGG GAAAGGTGGA AAAAAGAAGA AAAATAAAAA CTCTTCTAAA
    CCACAGAAAA ACAATGGATC CACTTGGGCC AATGTCCCTC TACCTCCCCC CCCAGTCCAG
    CCCCTTCCTG GCACGGAGCT GGAACACTAT GCAGTGGAAC AACAAGAAAA TGGCTATGAC
    AGTGATAGCT GGTGCCCACC ATTGCCAGTA CAAACTTACT TACACCAAGG TCTGGAAGAT
    GAACTGGAAG AAGATGATGA TAGGGTCCCA ACACCTCCTG TTCGAGGCGT GGCTTCTTCT
    CCTGCTATCT CCTTTGGACA GCAGTCCACT GCAACTCTTA CTCCATCCCC ACGGGAAGAG
    ATGCAACCCA TGCTGCAGGC TCACCTGGAT GAGTTGACAA GAGCCTATCA GTTTGATATA
    GCAAAACAAA CATGGCACAT TCAAAGCAAT AATCAACCTC CACAGCCTCC AGTTCCACCG
    TTAGGTTATG TGTCTGGAGC CTTGATTTCT GATTTGGAAA CGGATGTTGC AGATGATGAT
    GCCGACGACG AAGAGGAAGC TTTAGAAATC CCCAGGCCCC TGAGAGCACT GGACCAGACT
    CCTGGATCCA GCATGGACAA TCTAGACAGC TCTGTGACAG GAAAAGCCTT TACCTCCTCT
    CAAAGACCTC GACCTACCAG CCCATTTTCT ACTGACAGTA ACACCAGTGC AGCCCTGAGT
    CAAAGTCAGA GGCCTCGGCC CACTAAAAAA CACAAGGGAG GGCGGATGGA CCAACAACCA
    GCATTGCCTC ATCGAAGGGA AGGAATGACA GATGATCTTC CACCACCACC AGATCCCCCG
    CCAGGTCAGG GTTTAAGGCA GCAAATAGGC CCGAGCCAGC AGGCTGGTAA CGTGGAAAAC
    TCAGCAGAGA GAAAAGGAAG CTCTCTAGAG AGACAACATG CATCCAGCTT AGAAGACACA
    AAGAGCTCAT TGGATTGTCC AGCTAGAACC TCCCTAGAGT GGCAGCGACA AACCCAGGAA
    TGGATAAGCT CCACAGAACG ACAAGAAGAT ATACGGAAAG CCCCACACAA ACAAGGTGTC
    GGATCAGAGG AGGCCTTGGT GCCCTATAGC AAGCCCAGTT TCCCATCTCC AGGTGGCCAC
    AGCTCATCAG GAACAGCTTC TTCTAAGGGA TCCACTGGAC CTAGGAAAAC CGAGGTGTTG
    AGAGCAGGCC ACCAGCGCAA TGCCAGCGAC CTTCTTGACA TAGGATATAT GGGCTCCAAC
    AGTCAAGGAC AGTTTACAGG TGAATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016862481.1
    CDS300..4769
    Translation

    Target ORF information:

    RefSeq Version XM_017006992.1
    Organism Homo sapiens(human)
    Definition Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017006992.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCCAGAA GACATGAACG TGTCACTAGA AGGATGTGGA CATGGGCTCC GGGACTGTTG 
    ATGATGACTG TGGTGTTTTG GGGTCATCAG GGGAATGGAC AAGGCCAAGG ATCGCGTCTT
    CGCCAGGAGG ACTTTCCCCC GCGGATTGTG GAGCATCCTT CCGATGTCAT CGTCTCTAAG
    GGCGAGCCCA CGACTCTGAA CTGCAAGGCG GAGGGCCGGC CAACGCCCAC CATTGAGTGG
    TACAAAGATG GGGAGCGAGT GGAGACTGAC AAGGACGATC CCCGGTCCCA CAGGATGCTT
    CTGCCCAGCG GATCCTTATT CTTCTTGCGC ATCGTGCACG GGCGCAGGAG TAAACCTGAT
    GAAGGAAGCT ACGTTTGTGT TGCGAGGAAC TATCTTGGTG AAGCAGTGAG TCGAAATGCG
    TCTCTGGAAG TGGCATTGTT ACGAGATGAC TTCCGACAAA ACCCCACAGA TGTTGTAGTG
    GCAGCTGGAG AGCCTGCAAT CCTGGAGTGC CAGCCTCCCC GGGGACACCC AGAACCCACC
    ATCTACTGGA AAAAAGACAA AGTTCGAATT GATGACAAGG AAGAAAGAAT AAGTATCCGT
    GGTGGAAAAC TGATGATCTC CAATACCAGG AAAAGTGATG CAGGGATGTA TACTTGTGTT
    GGTACCAATA TGGTGGGAGA AAGGGACAGT GACCCAGCAG AGCTGACTGT CTTTGAACGA
    CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTACTGG AGGAAGAAGC TGTAGAATTT
    CGTTGTCAAG TCCAAGGAGA TCCTCAACCA ACTGTGAGGT GGAAAAAGGA TGATGCAGAC
    TTGCCAAGAG GAAGGTATGA CATCAAAGAC GATTACACAC TAAGAATTAA AAAGACCATG
    AGTACAGATG AAGGCACCTA TATGTGTATT GCTGAGAATC GGGTTGGAAA AATGGAAGCC
    TCTGCTACAC TCACCGTCCG AGCTCGCCCT GTTGCTCCCC CACAGTTTGT GGTTCGGCCA
    AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC
    CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA
    CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC
    ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT
    TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT
    CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA
    GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT
    AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT
    TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT
    GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC
    CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG
    TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC
    AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT
    ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC
    CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA
    CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT
    CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC
    CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG
    ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC
    AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA
    GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA
    CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG
    GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA
    GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA
    ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA
    GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC
    ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC
    TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG
    TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA
    GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC
    TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT
    GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA
    GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT
    ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA
    CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG
    AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG
    AACAAAGGTA ACAATGGTGG GAAAGGTGGA AAAAAGAAGA AAAATAAAAA CTCTTCTAAA
    CCACAGAAAA ACAATGGATC CACTTGGGCC AATGTCCCTC TACCTCCCCC CCCAGTCCAG
    CCCCTTCCTG GCACGGAGCT GGAACACTAT GCAGTGGAAC AACAAGAAAA TGGCTATGAC
    AGTGATAGCT GGTGCCCACC ATTGCCAGTA CAAACTTACT TACACCAAGG TCTGGAAGAT
    GAACTGGAAG AAGATGATGA TAGGGTCCCA ACACCTCCTG TTCGAGGCGT GGCTTCTTCT
    CCTGCTATCT CCTTTGGACA GCAGTCCACT GCAACTCTTA CTCCATCCCC ACGGGAAGAG
    ATGCAACCCA TGCTGCAGGC TCACCTGGAT GAGTTGACAA GAGCCTATCA GTTTGATATA
    GCAAAACAAA CATGGCACAT TCAAAGCAAT AATCAACCTC CACAGCCTCC AGTTCCACCG
    TTAGGTTATG TGTCTGGAGC CTTGATTTCT GATTTGGAAA CGGATGTTGC AGATGATGAT
    GCCGACGACG AAGAGGAAGC TTTAGAAATC CCCAGGCCCC TGAGAGCACT GGACCAGACT
    CCTGGATCCA GCATGGACAA TCTAGACAGC TCTGTGACAG GAAAAGCCTT TACCTCCTCT
    CAAAGACCTC GACCTACCAG CCCATTTTCT ACTGACAGTA ACACCAGTGC AGCCCTGAGT
    CAAAGTCAGA GGCCTCGGCC CACTAAAAAA CACAAGGGAG GGCGGATGGA CCAACAACCA
    GCATTGCCTC ATCGAAGGGA AGGAATGACA GATGATCTTC CACCACCACC AGATCCCCCG
    CCAGGTCAGG GTTTAAGGCA GCAAATAGGC CCGAGCCAGC AGGCTGGTAA CGTGGAAAAC
    TCAGCAGAGA GAAAAGGAAG CTCTCTAGAG AGACAACATG CATCCAGCTT AGAAGACACA
    AAGAGCTCAT TGGATTGTCC AGCTAGAACC TCCCTAGAGT GGCAGCGACA AACCCAGGAA
    TGGATAAGCT CCACAGAACG ACAAGAAGAT ATACGGAAAG CCCCACACAA ACAAGGTGTC
    GGATCAGAGG AGGCCTTGGT GCCCTATAGC AAGCCCAGTT TCCCATCTCC AGGTGGCCAC
    AGCTCATCAG GAACAGCTTC TTCTAAGGGA TCCACTGGAC CTAGGAAAAC CGAGGTGTTG
    AGAGCAGGCC ACCAGCGCAA TGCCAGCGAC CTTCTTGACA TAGGATATAT GGGCTCCAAC
    AGTCAAGGAC AGTTTACAGG TGAATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94467
    Clone ID Related Accession (Same CDS sequence) XM_017006993.1
    Accession Version XM_017006993.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4410bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product roundabout homolog 2 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC
    ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC
    ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC
    CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG
    AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA
    GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG
    TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA
    GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG
    GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT
    AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA
    AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCCCC CACAGTTTGT GGTTCGGCCA
    AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC
    CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA
    CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC
    ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT
    TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT
    CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA
    GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT
    AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT
    TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT
    GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC
    CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG
    TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC
    AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT
    ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC
    CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA
    CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT
    CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC
    CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG
    ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC
    AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA
    GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA
    CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG
    GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA
    GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA
    ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA
    GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC
    ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC
    TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG
    TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA
    GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC
    TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT
    GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA
    GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT
    ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA
    CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG
    AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG
    AACAAAGGTA ACAATGGTGG GAAAGGTGGA AAAAAGAAGA AAAATAAAAA CTCTTCTAAA
    CCACAGAAAA ACAATGGATC CACTTGGGCC AATGTCCCTC TACCTCCCCC CCCAGTCCAG
    CCCCTTCCTG GCACGGAGCT GGAACACTAT GCAGTGGAAC AACAAGAAAA TGGCTATGAC
    AGTGATAGCT GGTGCCCACC ATTGCCAGTA CAAACTTACT TACACCAAGG TCTGGAAGAT
    GAACTGGAAG AAGATGATGA TAGGGTCCCA ACACCTCCTG TTCGAGGCGT GGCTTCTTCT
    CCTGCTATCT CCTTTGGACA GCAGTCCACT GCAACTCTTA CTCCATCCCC ACGGGAAGAG
    ATGCAACCCA TGCTGCAGGC TCACCTGGAT GAGTTGACAA GAGCCTATCA GTTTGATATA
    GCAAAACAAA CATGGCACAT TCAAAGCAAT AATCAACCTC CACAGCCTCC AGTTCCACCG
    TTAGGTTATG TGTCTGGAGC CTTGATTTCT GATTTGGAAA CGGATGTTGC AGATGATGAT
    GCCGACGACG AAGAGGAAGC TTTAGAAATC CCCAGGCCCC TGAGAGCACT GGACCAGACT
    CCTGGATCCA GCATGGACAA TCTAGACAGC TCTGTGACAG GAAAAGCCTT TACCTCCTCT
    CAAAGACCTC GACCTACCAG CCCATTTTCT ACTGACAGTA ACACCAGTGC AGCCCTGAGT
    CAAAGTCAGA GGCCTCGGCC CACTAAAAAA CACAAGGGAG GGCGGATGGA CCAACAACCA
    GCATTGCCTC ATCGAAGGGA AGGAATGACA GATGATCTTC CACCACCACC AGATCCCCCG
    CCAGGTCAGG GTTTAAGGCA GCAAATAGGC CCGAGCCAGC AGGCTGGTAA CGTGGAAAAC
    TCAGCAGAGA GAAAAGGAAG CTCTCTAGAG AGACAACATG CATCCAGCTT AGAAGACACA
    AAGAGCTCAT TGGATTGTCC AGCTAGAACC TCCCTAGAGT GGCAGCGACA AACCCAGGAA
    TGGATAAGCT CCACAGAACG ACAAGAAGAT ATACGGAAAG CCCCACACAA ACAAGGTGTC
    GGATCAGAGG AGGCCTTGGT GCCCTATAGC AAGCCCAGTT TCCCATCTCC AGGTGGCCAC
    AGCTCATCAG GAACAGCTTC TTCTAAGGGA TCCACTGGAC CTAGGAAAAC CGAGGTGTTG
    AGAGCAGGCC ACCAGCGCAA TGCCAGCGAC CTTCTTGACA TAGGATATAT GGGCTCCAAC
    AGTCAAGGAC AGTTTACAGG TGAATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016862482.1
    CDS692..5101
    Translation

    Target ORF information:

    RefSeq Version XM_017006993.1
    Organism Homo sapiens(human)
    Definition Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017006993.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC
    ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC
    ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC
    CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG
    AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA
    GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG
    TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA
    GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG
    GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT
    AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA
    AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCCCC CACAGTTTGT GGTTCGGCCA
    AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC
    CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA
    CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC
    ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT
    TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT
    CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA
    GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT
    AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT
    TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT
    GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC
    CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG
    TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC
    AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT
    ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC
    CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA
    CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT
    CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC
    CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG
    ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC
    AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA
    GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA
    CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG
    GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA
    GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA
    ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA
    GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC
    ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC
    TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG
    TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA
    GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC
    TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT
    GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA
    GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT
    ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA
    CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG
    AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG
    AACAAAGGTA ACAATGGTGG GAAAGGTGGA AAAAAGAAGA AAAATAAAAA CTCTTCTAAA
    CCACAGAAAA ACAATGGATC CACTTGGGCC AATGTCCCTC TACCTCCCCC CCCAGTCCAG
    CCCCTTCCTG GCACGGAGCT GGAACACTAT GCAGTGGAAC AACAAGAAAA TGGCTATGAC
    AGTGATAGCT GGTGCCCACC ATTGCCAGTA CAAACTTACT TACACCAAGG TCTGGAAGAT
    GAACTGGAAG AAGATGATGA TAGGGTCCCA ACACCTCCTG TTCGAGGCGT GGCTTCTTCT
    CCTGCTATCT CCTTTGGACA GCAGTCCACT GCAACTCTTA CTCCATCCCC ACGGGAAGAG
    ATGCAACCCA TGCTGCAGGC TCACCTGGAT GAGTTGACAA GAGCCTATCA GTTTGATATA
    GCAAAACAAA CATGGCACAT TCAAAGCAAT AATCAACCTC CACAGCCTCC AGTTCCACCG
    TTAGGTTATG TGTCTGGAGC CTTGATTTCT GATTTGGAAA CGGATGTTGC AGATGATGAT
    GCCGACGACG AAGAGGAAGC TTTAGAAATC CCCAGGCCCC TGAGAGCACT GGACCAGACT
    CCTGGATCCA GCATGGACAA TCTAGACAGC TCTGTGACAG GAAAAGCCTT TACCTCCTCT
    CAAAGACCTC GACCTACCAG CCCATTTTCT ACTGACAGTA ACACCAGTGC AGCCCTGAGT
    CAAAGTCAGA GGCCTCGGCC CACTAAAAAA CACAAGGGAG GGCGGATGGA CCAACAACCA
    GCATTGCCTC ATCGAAGGGA AGGAATGACA GATGATCTTC CACCACCACC AGATCCCCCG
    CCAGGTCAGG GTTTAAGGCA GCAAATAGGC CCGAGCCAGC AGGCTGGTAA CGTGGAAAAC
    TCAGCAGAGA GAAAAGGAAG CTCTCTAGAG AGACAACATG CATCCAGCTT AGAAGACACA
    AAGAGCTCAT TGGATTGTCC AGCTAGAACC TCCCTAGAGT GGCAGCGACA AACCCAGGAA
    TGGATAAGCT CCACAGAACG ACAAGAAGAT ATACGGAAAG CCCCACACAA ACAAGGTGTC
    GGATCAGAGG AGGCCTTGGT GCCCTATAGC AAGCCCAGTT TCCCATCTCC AGGTGGCCAC
    AGCTCATCAG GAACAGCTTC TTCTAAGGGA TCCACTGGAC CTAGGAAAAC CGAGGTGTTG
    AGAGCAGGCC ACCAGCGCAA TGCCAGCGAC CTTCTTGACA TAGGATATAT GGGCTCCAAC
    AGTCAAGGAC AGTTTACAGG TGAATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94468
    Clone ID Related Accession (Same CDS sequence) XM_017006994.1
    Accession Version XM_017006994.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4338bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product roundabout homolog 2 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
    CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT
    TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT
    CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC
    GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA
    AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC
    CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG
    TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA
    CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT
    CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC
    CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT
    GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT
    ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT
    AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC
    CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC
    ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG
    CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG
    GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT
    CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT
    GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG
    CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA
    GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG
    GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC
    GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT
    GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT
    CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG
    CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC
    GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT
    GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGCCT TACTTTACAT TCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCTCAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA ATAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCCAC TTGGGCCAAT
    GTCCCTCTAC CTCCCCCCCC AGTCCAGCCC CTTCCTGGCA CGGAGCTGGA ACACTATGCA
    GTGGAACAAC AAGAAAATGG CTATGACAGT GATAGCTGGT GCCCACCATT GCCAGTACAA
    ACTTACTTAC ACCAAGGTCT GGAAGATGAA CTGGAAGAAG ATGATGATAG GGTCCCAACA
    CCTCCTGTTC GAGGCGTGGC TTCTTCTCCT GCTATCTCCT TTGGACAGCA GTCCACTGCA
    ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAGGCTCA CCTGGATGAG
    TTGACAAGAG CCTATCAGTT TGATATAGCA AAACAAACAT GGCACATTCA AAGCAATAAT
    CAACCTCCAC AGCCTCCAGT TCCACCGTTA GGTTATGTGT CTGGAGCCTT GATTTCTGAT
    TTGGAAACGG ATGTTGCAGA TGATGATGCC GACGACGAAG AGGAAGCTTT AGAAATCCCC
    AGGCCCCTGA GAGCACTGGA CCAGACTCCT GGATCCAGCA TGGACAATCT AGACAGCTCT
    GTGACAGGAA AAGCCTTTAC CTCCTCTCAA AGACCTCGAC CTACCAGCCC ATTTTCTACT
    GACAGTAACA CCAGTGCAGC CCTGAGTCAA AGTCAGAGGC CTCGGCCCAC TAAAAAACAC
    AAGGGAGGGC GGATGGACCA ACAACCAGCA TTGCCTCATC GAAGGGAAGG AATGACAGAT
    GAGGAGGCCT TGGTGCCCTA TAGCAAGCCC AGTTTCCCAT CTCCAGGTGG CCACAGCTCA
    TCAGGAACAG CTTCTTCTAA GGGATCCACT GGACCTAGGA AAACCGAGGT GTTGAGAGCA
    GGCCACCAGC GCAATGCCAG CGACCTTCTT GACATAGGAT ATATGGGCTC CAACAGTCAA
    GGACAGTTTA CAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016862483.1
    CDS400..4737
    Translation

    Target ORF information:

    RefSeq Version XM_017006994.1
    Organism Homo sapiens(human)
    Definition Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017006994.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
    CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT
    TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT
    CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC
    GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA
    AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC
    CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG
    TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA
    CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT
    CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC
    CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT
    GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT
    ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT
    AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC
    CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC
    ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG
    CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG
    GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT
    CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT
    GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG
    CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA
    GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG
    GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC
    GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT
    GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT
    CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG
    CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC
    GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT
    GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGCCT TACTTTACAT TCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCTCAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA ATAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCCAC TTGGGCCAAT
    GTCCCTCTAC CTCCCCCCCC AGTCCAGCCC CTTCCTGGCA CGGAGCTGGA ACACTATGCA
    GTGGAACAAC AAGAAAATGG CTATGACAGT GATAGCTGGT GCCCACCATT GCCAGTACAA
    ACTTACTTAC ACCAAGGTCT GGAAGATGAA CTGGAAGAAG ATGATGATAG GGTCCCAACA
    CCTCCTGTTC GAGGCGTGGC TTCTTCTCCT GCTATCTCCT TTGGACAGCA GTCCACTGCA
    ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAGGCTCA CCTGGATGAG
    TTGACAAGAG CCTATCAGTT TGATATAGCA AAACAAACAT GGCACATTCA AAGCAATAAT
    CAACCTCCAC AGCCTCCAGT TCCACCGTTA GGTTATGTGT CTGGAGCCTT GATTTCTGAT
    TTGGAAACGG ATGTTGCAGA TGATGATGCC GACGACGAAG AGGAAGCTTT AGAAATCCCC
    AGGCCCCTGA GAGCACTGGA CCAGACTCCT GGATCCAGCA TGGACAATCT AGACAGCTCT
    GTGACAGGAA AAGCCTTTAC CTCCTCTCAA AGACCTCGAC CTACCAGCCC ATTTTCTACT
    GACAGTAACA CCAGTGCAGC CCTGAGTCAA AGTCAGAGGC CTCGGCCCAC TAAAAAACAC
    AAGGGAGGGC GGATGGACCA ACAACCAGCA TTGCCTCATC GAAGGGAAGG AATGACAGAT
    GAGGAGGCCT TGGTGCCCTA TAGCAAGCCC AGTTTCCCAT CTCCAGGTGG CCACAGCTCA
    TCAGGAACAG CTTCTTCTAA GGGATCCACT GGACCTAGGA AAACCGAGGT GTTGAGAGCA
    GGCCACCAGC GCAATGCCAG CGACCTTCTT GACATAGGAT ATATGGGCTC CAACAGTCAA
    GGACAGTTTA CAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94469
    Clone ID Related Accession (Same CDS sequence) XM_017006995.1
    Accession Version XM_017006995.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4329bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product roundabout homolog 2 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
    CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT
    TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT
    CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC
    GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA
    AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC
    CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG
    TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA
    CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT
    CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC
    CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT
    GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT
    ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT
    AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC
    CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC
    ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG
    CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG
    GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT
    CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT
    GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG
    CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA
    GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG
    GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC
    GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT
    GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT
    CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG
    CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC
    GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT
    GGTTATTCTC TACCTGATCA GAACAAAGCC TTACTTTACA TTCCTGACTA CCGATTGGCT
    GAGGGATTGT CTAATAGAAT GCCACACAAC CAGTCTCAGG ATTTCAGCAC CACCAGCTCT
    CACAACAGCT CAGAAAGGAG TGGCAGTCTC TCAGGTGGGA AAGGTGGAAA AAAGAAGAAA
    AATAAAAACT CTTCTAAACC ACAGAAAAAC AATGGATCCA CTTGGGCCAA TGTCCCTCTA
    CCTCCCCCCC CAGTCCAGCC CCTTCCTGGC ACGGAGCTGG AACACTATGC AGTGGAACAA
    CAAGAAAATG GCTATGACAG TGATAGCTGG TGCCCACCAT TGCCAGTACA AACTTACTTA
    CACCAAGGTC TGGAAGATGA ACTGGAAGAA GATGATGATA GGGTCCCAAC ACCTCCTGTT
    CGAGGCGTGG CTTCTTCTCC TGCTATCTCC TTTGGACAGC AGTCCACTGC AACTCTTACT
    CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAGGCTC ACCTGGATGA GTTGACAAGA
    GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAA TCAACCTCCA
    CAGCCTCCAG TTCCACCGTT AGGTTATGTG TCTGGAGCCT TGATTTCTGA TTTGGAAACG
    GATGTTGCAG ATGATGATGC CGACGACGAA GAGGAAGCTT TAGAAATCCC CAGGCCCCTG
    AGAGCACTGG ACCAGACTCC TGGATCCAGC ATGGACAATC TAGACAGCTC TGTGACAGGA
    AAAGCCTTTA CCTCCTCTCA AAGACCTCGA CCTACCAGCC CATTTTCTAC TGACAGTAAC
    ACCAGTGCAG CCCTGAGTCA AAGTCAGAGG CCTCGGCCCA CTAAAAAACA CAAGGGAGGG
    CGGATGGACC AACAACCAGC ATTGCCTCAT CGAAGGGAAG GAATGACAGA TGAGGAGGCC
    TTGGTGCCCT ATAGCAAGCC CAGTTTCCCA TCTCCAGGTG GCCACAGCTC ATCAGGAACA
    GCTTCTTCTA AGGGATCCAC TGGACCTAGG AAAACCGAGG TGTTGAGAGC AGGCCACCAG
    CGCAATGCCA GCGACCTTCT TGACATAGGA TATATGGGCT CCAACAGTCA AGGACAGTTT
    ACAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016862484.1
    CDS400..4728
    Translation

    Target ORF information:

    RefSeq Version XM_017006995.1
    Organism Homo sapiens(human)
    Definition Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017006995.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
    CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT
    TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT
    CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC
    GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA
    AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC
    CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG
    TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA
    CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT
    CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC
    CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT
    GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT
    ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT
    AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC
    CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC
    ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG
    CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG
    GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT
    CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT
    GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG
    CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA
    GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG
    GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC
    GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT
    GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT
    CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG
    CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC
    GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT
    GGTTATTCTC TACCTGATCA GAACAAAGCC TTACTTTACA TTCCTGACTA CCGATTGGCT
    GAGGGATTGT CTAATAGAAT GCCACACAAC CAGTCTCAGG ATTTCAGCAC CACCAGCTCT
    CACAACAGCT CAGAAAGGAG TGGCAGTCTC TCAGGTGGGA AAGGTGGAAA AAAGAAGAAA
    AATAAAAACT CTTCTAAACC ACAGAAAAAC AATGGATCCA CTTGGGCCAA TGTCCCTCTA
    CCTCCCCCCC CAGTCCAGCC CCTTCCTGGC ACGGAGCTGG AACACTATGC AGTGGAACAA
    CAAGAAAATG GCTATGACAG TGATAGCTGG TGCCCACCAT TGCCAGTACA AACTTACTTA
    CACCAAGGTC TGGAAGATGA ACTGGAAGAA GATGATGATA GGGTCCCAAC ACCTCCTGTT
    CGAGGCGTGG CTTCTTCTCC TGCTATCTCC TTTGGACAGC AGTCCACTGC AACTCTTACT
    CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAGGCTC ACCTGGATGA GTTGACAAGA
    GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAA TCAACCTCCA
    CAGCCTCCAG TTCCACCGTT AGGTTATGTG TCTGGAGCCT TGATTTCTGA TTTGGAAACG
    GATGTTGCAG ATGATGATGC CGACGACGAA GAGGAAGCTT TAGAAATCCC CAGGCCCCTG
    AGAGCACTGG ACCAGACTCC TGGATCCAGC ATGGACAATC TAGACAGCTC TGTGACAGGA
    AAAGCCTTTA CCTCCTCTCA AAGACCTCGA CCTACCAGCC CATTTTCTAC TGACAGTAAC
    ACCAGTGCAG CCCTGAGTCA AAGTCAGAGG CCTCGGCCCA CTAAAAAACA CAAGGGAGGG
    CGGATGGACC AACAACCAGC ATTGCCTCAT CGAAGGGAAG GAATGACAGA TGAGGAGGCC
    TTGGTGCCCT ATAGCAAGCC CAGTTTCCCA TCTCCAGGTG GCCACAGCTC ATCAGGAACA
    GCTTCTTCTA AGGGATCCAC TGGACCTAGG AAAACCGAGG TGTTGAGAGC AGGCCACCAG
    CGCAATGCCA GCGACCTTCT TGACATAGGA TATATGGGCT CCAACAGTCA AGGACAGTTT
    ACAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94470
    Clone ID Related Accession (Same CDS sequence) XM_017006996.1
    Accession Version XM_017006996.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4323bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product roundabout homolog 2 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCCAGAA GACATGAACG TGTCACTAGA AGGATGTGGA CATGGGCTCC GGGACTGTTG 
    ATGATGACTG TGGTGTTTTG GGGTCATCAG GGGAATGGAC AAGGCCAAGG ATCGCGTCTT
    CGCCAGGAGG ACTTTCCCCC GCGGATTGTG GAGCATCCTT CCGATGTCAT CGTCTCTAAG
    GGCGAGCCCA CGACTCTGAA CTGCAAGGCG GAGGGCCGGC CAACGCCCAC CATTGAGTGG
    TACAAAGATG GGGAGCGAGT GGAGACTGAC AAGGACGATC CCCGGTCCCA CAGGATGCTT
    CTGCCCAGCG GATCCTTATT CTTCTTGCGC ATCGTGCACG GGCGCAGGAG TAAACCTGAT
    GAAGGAAGCT ACGTTTGTGT TGCGAGGAAC TATCTTGGTG AAGCAGTGAG TCGAAATGCG
    TCTCTGGAAG TGGCATTGTT ACGAGATGAC TTCCGACAAA ACCCCACAGA TGTTGTAGTG
    GCAGCTGGAG AGCCTGCAAT CCTGGAGTGC CAGCCTCCCC GGGGACACCC AGAACCCACC
    ATCTACTGGA AAAAAGACAA AGTTCGAATT GATGACAAGG AAGAAAGAAT AAGTATCCGT
    GGTGGAAAAC TGATGATCTC CAATACCAGG AAAAGTGATG CAGGGATGTA TACTTGTGTT
    GGTACCAATA TGGTGGGAGA AAGGGACAGT GACCCAGCAG AGCTGACTGT CTTTGAACGA
    CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTACTGG AGGAAGAAGC TGTAGAATTT
    CGTTGTCAAG TCCAAGGAGA TCCTCAACCA ACTGTGAGGT GGAAAAAGGA TGATGCAGAC
    TTGCCAAGAG GAAGGTATGA CATCAAAGAC GATTACACAC TAAGAATTAA AAAGACCATG
    AGTACAGATG AAGGCACCTA TATGTGTATT GCTGAGAATC GGGTTGGAAA AATGGAAGCC
    TCTGCTACAC TCACCGTCCG AGCTCGCCCT GTTGCTCCCC CACAGTTTGT GGTTCGGCCA
    AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC
    CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA
    CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC
    ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT
    TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT
    CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA
    GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT
    AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT
    TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT
    GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC
    CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG
    TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC
    AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT
    ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC
    CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA
    CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT
    CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC
    CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG
    ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC
    AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA
    GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA
    CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG
    GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA
    GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA
    ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA
    GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC
    ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC
    TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG
    TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA
    GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC
    TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT
    GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA
    GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT
    ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA
    CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG
    AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG
    AACAAAGGTA ACAATGCCTT ACTTTACATT CCTGACTACC GATTGGCTGA GGGATTGTCT
    AATAGAATGC CACACAACCA GTCTCAGGAT TTCAGCACCA CCAGCTCTCA CAACAGCTCA
    GAAAGGAGTG GCAGTCTCTC AGGTGGGAAA GGTGGAAAAA AGAAGAAAAA TAAAAACTCT
    TCTAAACCAC AGAAAAACAA TGGATCCACT TGGGCCAATG TCCCTCTACC TCCCCCCCCA
    GTCCAGCCCC TTCCTGGCAC GGAGCTGGAA CACTATGCAG TGGAACAACA AGAAAATGGC
    TATGACAGTG ATAGCTGGTG CCCACCATTG CCAGTACAAA CTTACTTACA CCAAGGTCTG
    GAAGATGAAC TGGAAGAAGA TGATGATAGG GTCCCAACAC CTCCTGTTCG AGGCGTGGCT
    TCTTCTCCTG CTATCTCCTT TGGACAGCAG TCCACTGCAA CTCTTACTCC ATCCCCACGG
    GAAGAGATGC AACCCATGCT GCAGGCTCAC CTGGATGAGT TGACAAGAGC CTATCAGTTT
    GATATAGCAA AACAAACATG GCACATTCAA AGCAATAATC AACCTCCACA GCCTCCAGTT
    CCACCGTTAG GTTATGTGTC TGGAGCCTTG ATTTCTGATT TGGAAACGGA TGTTGCAGAT
    GATGATGCCG ACGACGAAGA GGAAGCTTTA GAAATCCCCA GGCCCCTGAG AGCACTGGAC
    CAGACTCCTG GATCCAGCAT GGACAATCTA GACAGCTCTG TGACAGGAAA AGCCTTTACC
    TCCTCTCAAA GACCTCGACC TACCAGCCCA TTTTCTACTG ACAGTAACAC CAGTGCAGCC
    CTGAGTCAAA GTCAGAGGCC TCGGCCCACT AAAAAACACA AGGGAGGGCG GATGGACCAA
    CAACCAGCAT TGCCTCATCG AAGGGAAGGA ATGACAGATG AGGAGGCCTT GGTGCCCTAT
    AGCAAGCCCA GTTTCCCATC TCCAGGTGGC CACAGCTCAT CAGGAACAGC TTCTTCTAAG
    GGATCCACTG GACCTAGGAA AACCGAGGTG TTGAGAGCAG GCCACCAGCG CAATGCCAGC
    GACCTTCTTG ACATAGGATA TATGGGCTCC AACAGTCAAG GACAGTTTAC AGGTGAATTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016862485.1
    CDS300..4622
    Translation

    Target ORF information:

    RefSeq Version XM_017006996.1
    Organism Homo sapiens(human)
    Definition Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017006996.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCCAGAA GACATGAACG TGTCACTAGA AGGATGTGGA CATGGGCTCC GGGACTGTTG 
    ATGATGACTG TGGTGTTTTG GGGTCATCAG GGGAATGGAC AAGGCCAAGG ATCGCGTCTT
    CGCCAGGAGG ACTTTCCCCC GCGGATTGTG GAGCATCCTT CCGATGTCAT CGTCTCTAAG
    GGCGAGCCCA CGACTCTGAA CTGCAAGGCG GAGGGCCGGC CAACGCCCAC CATTGAGTGG
    TACAAAGATG GGGAGCGAGT GGAGACTGAC AAGGACGATC CCCGGTCCCA CAGGATGCTT
    CTGCCCAGCG GATCCTTATT CTTCTTGCGC ATCGTGCACG GGCGCAGGAG TAAACCTGAT
    GAAGGAAGCT ACGTTTGTGT TGCGAGGAAC TATCTTGGTG AAGCAGTGAG TCGAAATGCG
    TCTCTGGAAG TGGCATTGTT ACGAGATGAC TTCCGACAAA ACCCCACAGA TGTTGTAGTG
    GCAGCTGGAG AGCCTGCAAT CCTGGAGTGC CAGCCTCCCC GGGGACACCC AGAACCCACC
    ATCTACTGGA AAAAAGACAA AGTTCGAATT GATGACAAGG AAGAAAGAAT AAGTATCCGT
    GGTGGAAAAC TGATGATCTC CAATACCAGG AAAAGTGATG CAGGGATGTA TACTTGTGTT
    GGTACCAATA TGGTGGGAGA AAGGGACAGT GACCCAGCAG AGCTGACTGT CTTTGAACGA
    CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTACTGG AGGAAGAAGC TGTAGAATTT
    CGTTGTCAAG TCCAAGGAGA TCCTCAACCA ACTGTGAGGT GGAAAAAGGA TGATGCAGAC
    TTGCCAAGAG GAAGGTATGA CATCAAAGAC GATTACACAC TAAGAATTAA AAAGACCATG
    AGTACAGATG AAGGCACCTA TATGTGTATT GCTGAGAATC GGGTTGGAAA AATGGAAGCC
    TCTGCTACAC TCACCGTCCG AGCTCGCCCT GTTGCTCCCC CACAGTTTGT GGTTCGGCCA
    AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC
    CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA
    CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC
    ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT
    TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT
    CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA
    GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT
    AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT
    TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT
    GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC
    CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG
    TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC
    AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT
    ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC
    CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA
    CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT
    CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC
    CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG
    ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC
    AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA
    GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA
    CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG
    GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA
    GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA
    ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA
    GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC
    ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC
    TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG
    TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA
    GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC
    TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT
    GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA
    GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT
    ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA
    CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG
    AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG
    AACAAAGGTA ACAATGCCTT ACTTTACATT CCTGACTACC GATTGGCTGA GGGATTGTCT
    AATAGAATGC CACACAACCA GTCTCAGGAT TTCAGCACCA CCAGCTCTCA CAACAGCTCA
    GAAAGGAGTG GCAGTCTCTC AGGTGGGAAA GGTGGAAAAA AGAAGAAAAA TAAAAACTCT
    TCTAAACCAC AGAAAAACAA TGGATCCACT TGGGCCAATG TCCCTCTACC TCCCCCCCCA
    GTCCAGCCCC TTCCTGGCAC GGAGCTGGAA CACTATGCAG TGGAACAACA AGAAAATGGC
    TATGACAGTG ATAGCTGGTG CCCACCATTG CCAGTACAAA CTTACTTACA CCAAGGTCTG
    GAAGATGAAC TGGAAGAAGA TGATGATAGG GTCCCAACAC CTCCTGTTCG AGGCGTGGCT
    TCTTCTCCTG CTATCTCCTT TGGACAGCAG TCCACTGCAA CTCTTACTCC ATCCCCACGG
    GAAGAGATGC AACCCATGCT GCAGGCTCAC CTGGATGAGT TGACAAGAGC CTATCAGTTT
    GATATAGCAA AACAAACATG GCACATTCAA AGCAATAATC AACCTCCACA GCCTCCAGTT
    CCACCGTTAG GTTATGTGTC TGGAGCCTTG ATTTCTGATT TGGAAACGGA TGTTGCAGAT
    GATGATGCCG ACGACGAAGA GGAAGCTTTA GAAATCCCCA GGCCCCTGAG AGCACTGGAC
    CAGACTCCTG GATCCAGCAT GGACAATCTA GACAGCTCTG TGACAGGAAA AGCCTTTACC
    TCCTCTCAAA GACCTCGACC TACCAGCCCA TTTTCTACTG ACAGTAACAC CAGTGCAGCC
    CTGAGTCAAA GTCAGAGGCC TCGGCCCACT AAAAAACACA AGGGAGGGCG GATGGACCAA
    CAACCAGCAT TGCCTCATCG AAGGGAAGGA ATGACAGATG AGGAGGCCTT GGTGCCCTAT
    AGCAAGCCCA GTTTCCCATC TCCAGGTGGC CACAGCTCAT CAGGAACAGC TTCTTCTAAG
    GGATCCACTG GACCTAGGAA AACCGAGGTG TTGAGAGCAG GCCACCAGCG CAATGCCAGC
    GACCTTCTTG ACATAGGATA TATGGGCTCC AACAGTCAAG GACAGTTTAC AGGTGAATTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94471
    Clone ID Related Accession (Same CDS sequence) XM_017006997.1
    Accession Version XM_017006997.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4317bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product roundabout homolog 2 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCCAGAA GACATGAACG TGTCACTAGA AGGATGTGGA CATGGGCTCC GGGACTGTTG 
    ATGATGACTG TGGTGTTTTG GGGTCATCAG GGGAATGGAC AAGGCCAAGG ATCGCGTCTT
    CGCCAGGAGG ACTTTCCCCC GCGGATTGTG GAGCATCCTT CCGATGTCAT CGTCTCTAAG
    GGCGAGCCCA CGACTCTGAA CTGCAAGGCG GAGGGCCGGC CAACGCCCAC CATTGAGTGG
    TACAAAGATG GGGAGCGAGT GGAGACTGAC AAGGACGATC CCCGGTCCCA CAGGATGCTT
    CTGCCCAGCG GATCCTTATT CTTCTTGCGC ATCGTGCACG GGCGCAGGAG TAAACCTGAT
    GAAGGAAGCT ACGTTTGTGT TGCGAGGAAC TATCTTGGTG AAGCAGTGAG TCGAAATGCG
    TCTCTGGAAG TGGCATTGTT ACGAGATGAC TTCCGACAAA ACCCCACAGA TGTTGTAGTG
    GCAGCTGGAG AGCCTGCAAT CCTGGAGTGC CAGCCTCCCC GGGGACACCC AGAACCCACC
    ATCTACTGGA AAAAAGACAA AGTTCGAATT GATGACAAGG AAGAAAGAAT AAGTATCCGT
    GGTGGAAAAC TGATGATCTC CAATACCAGG AAAAGTGATG CAGGGATGTA TACTTGTGTT
    GGTACCAATA TGGTGGGAGA AAGGGACAGT GACCCAGCAG AGCTGACTGT CTTTGAACGA
    CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTACTGG AGGAAGAAGC TGTAGAATTT
    CGTTGTCAAG TCCAAGGAGA TCCTCAACCA ACTGTGAGGT GGAAAAAGGA TGATGCAGAC
    TTGCCAAGAG GAAGGTATGA CATCAAAGAC GATTACACAC TAAGAATTAA AAAGACCATG
    AGTACAGATG AAGGCACCTA TATGTGTATT GCTGAGAATC GGGTTGGAAA AATGGAAGCC
    TCTGCTACAC TCACCGTCCG AGCTCGCCCT GTTGCTCCCC CACAGTTTGT GGTTCGGCCA
    AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC
    CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA
    CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC
    ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT
    TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT
    CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA
    GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT
    AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT
    TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT
    GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC
    CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG
    TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC
    AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT
    ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC
    CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA
    CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT
    CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC
    CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG
    ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC
    AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA
    GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA
    CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG
    GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA
    GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA
    ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA
    GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC
    ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC
    TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG
    TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA
    GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC
    TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT
    GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA
    GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT
    ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA
    CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG
    AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG
    AACAAAGGTA ACAATGCCTT ACTTTACATT CCTGACTACC GATTGGCTGA GGGATTGTCT
    AATAGAATGC CACACAACCA GTCTCAGGAT TTCAGCACCA CCAGCTCTCA CAACAGCTCA
    GAAAGGAGTG GCAGTCTCTC AGGTGGGAAA GGTGGAAAAA AGAAGAAAAA TAAAAACTCT
    TCTAAACCAC AGAAAAACAA TGGATCCACT TGGGCCAATG TCCCTCTACC TCCCCCCCCA
    GTCCAGCCCC TTCCTGGCAC GGAGCTGGAA CACTATGCAG TGGAACAACA AGAAAATGGC
    TATGACAGTG ATAGCTGGTG CCCACCATTG CCAGTACAAA CTTACTTACA CCAAGGTCTG
    GAAGATGAAC TGGAAGAAGA TGATGATAGG GTCCCAACAC CTCCTGTTCG AGGCGTGGCT
    TCTTCTCCTG CTATCTCCTT TGGACAGCAG TCCACTGCAA CTCTTACTCC ATCCCCACGG
    GAAGAGATGC AACCCATGCT GCAGGCTCAC CTGGATGAGT TGACAAGAGC CTATCAGTTT
    GATATAGCAA AACAAACATG GCACATTCAA AGCAATAATC AACCTCCACA GCCTCCAGTT
    CCACCGTTAG GTTATGTGTC TGGAGCCTTG ATTTCTGATT TGGAAACGGA TGTTGCAGAT
    GATGATGCCG ACGACGAAGA GGAAGCTTTA GAAATCCCCA GGCCCCTGAG AGCACTGGAC
    CAGACTCCTG GATCCAGCAT GGACAATCTA GACAGCTCTG TGACAGGAAA AGCCTTTACC
    TCCTCTCAAA GACCTCGACC TACCAGCCCA TTTTCTACTG ACAGTAACAC CAGTGCAGCC
    CTGAGTCAAA GTCAGAGGCC TCGGCCCACT AAAAAACACA AGGGAGGGCG GATGGACCAA
    CAACCAGCAT TGCCTCATCG AAGGGAAGGA ATGACAGATG AGGAGGCCTT GGTGCCCTAT
    AGCAAGCCCA GTTTCCCATC TCCAGGTGGC CACAGCTCAT CAGGAACAGC TTCTTCTAAG
    GGATCCACTG GACCTAGGAA AACCGAGGTG TTGAGAGCAG GCCACCAGCG CAATGCCAGC
    GACCTTCTTG ACATAGGATA TATGGGCTCC AACAGTCAAG GACAGTTTAC AGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016862486.1
    CDS300..4616
    Translation

    Target ORF information:

    RefSeq Version XM_017006997.1
    Organism Homo sapiens(human)
    Definition Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017006997.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCCAGAA GACATGAACG TGTCACTAGA AGGATGTGGA CATGGGCTCC GGGACTGTTG 
    ATGATGACTG TGGTGTTTTG GGGTCATCAG GGGAATGGAC AAGGCCAAGG ATCGCGTCTT
    CGCCAGGAGG ACTTTCCCCC GCGGATTGTG GAGCATCCTT CCGATGTCAT CGTCTCTAAG
    GGCGAGCCCA CGACTCTGAA CTGCAAGGCG GAGGGCCGGC CAACGCCCAC CATTGAGTGG
    TACAAAGATG GGGAGCGAGT GGAGACTGAC AAGGACGATC CCCGGTCCCA CAGGATGCTT
    CTGCCCAGCG GATCCTTATT CTTCTTGCGC ATCGTGCACG GGCGCAGGAG TAAACCTGAT
    GAAGGAAGCT ACGTTTGTGT TGCGAGGAAC TATCTTGGTG AAGCAGTGAG TCGAAATGCG
    TCTCTGGAAG TGGCATTGTT ACGAGATGAC TTCCGACAAA ACCCCACAGA TGTTGTAGTG
    GCAGCTGGAG AGCCTGCAAT CCTGGAGTGC CAGCCTCCCC GGGGACACCC AGAACCCACC
    ATCTACTGGA AAAAAGACAA AGTTCGAATT GATGACAAGG AAGAAAGAAT AAGTATCCGT
    GGTGGAAAAC TGATGATCTC CAATACCAGG AAAAGTGATG CAGGGATGTA TACTTGTGTT
    GGTACCAATA TGGTGGGAGA AAGGGACAGT GACCCAGCAG AGCTGACTGT CTTTGAACGA
    CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTACTGG AGGAAGAAGC TGTAGAATTT
    CGTTGTCAAG TCCAAGGAGA TCCTCAACCA ACTGTGAGGT GGAAAAAGGA TGATGCAGAC
    TTGCCAAGAG GAAGGTATGA CATCAAAGAC GATTACACAC TAAGAATTAA AAAGACCATG
    AGTACAGATG AAGGCACCTA TATGTGTATT GCTGAGAATC GGGTTGGAAA AATGGAAGCC
    TCTGCTACAC TCACCGTCCG AGCTCGCCCT GTTGCTCCCC CACAGTTTGT GGTTCGGCCA
    AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC
    CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA
    CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC
    ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT
    TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT
    CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA
    GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT
    AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT
    TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT
    GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC
    CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG
    TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC
    AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT
    ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC
    CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA
    CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT
    CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC
    CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG
    ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC
    AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA
    GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA
    CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG
    GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA
    GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA
    ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA
    GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC
    ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC
    TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG
    TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA
    GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC
    TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT
    GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA
    GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT
    ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA
    CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG
    AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG
    AACAAAGGTA ACAATGCCTT ACTTTACATT CCTGACTACC GATTGGCTGA GGGATTGTCT
    AATAGAATGC CACACAACCA GTCTCAGGAT TTCAGCACCA CCAGCTCTCA CAACAGCTCA
    GAAAGGAGTG GCAGTCTCTC AGGTGGGAAA GGTGGAAAAA AGAAGAAAAA TAAAAACTCT
    TCTAAACCAC AGAAAAACAA TGGATCCACT TGGGCCAATG TCCCTCTACC TCCCCCCCCA
    GTCCAGCCCC TTCCTGGCAC GGAGCTGGAA CACTATGCAG TGGAACAACA AGAAAATGGC
    TATGACAGTG ATAGCTGGTG CCCACCATTG CCAGTACAAA CTTACTTACA CCAAGGTCTG
    GAAGATGAAC TGGAAGAAGA TGATGATAGG GTCCCAACAC CTCCTGTTCG AGGCGTGGCT
    TCTTCTCCTG CTATCTCCTT TGGACAGCAG TCCACTGCAA CTCTTACTCC ATCCCCACGG
    GAAGAGATGC AACCCATGCT GCAGGCTCAC CTGGATGAGT TGACAAGAGC CTATCAGTTT
    GATATAGCAA AACAAACATG GCACATTCAA AGCAATAATC AACCTCCACA GCCTCCAGTT
    CCACCGTTAG GTTATGTGTC TGGAGCCTTG ATTTCTGATT TGGAAACGGA TGTTGCAGAT
    GATGATGCCG ACGACGAAGA GGAAGCTTTA GAAATCCCCA GGCCCCTGAG AGCACTGGAC
    CAGACTCCTG GATCCAGCAT GGACAATCTA GACAGCTCTG TGACAGGAAA AGCCTTTACC
    TCCTCTCAAA GACCTCGACC TACCAGCCCA TTTTCTACTG ACAGTAACAC CAGTGCAGCC
    CTGAGTCAAA GTCAGAGGCC TCGGCCCACT AAAAAACACA AGGGAGGGCG GATGGACCAA
    CAACCAGCAT TGCCTCATCG AAGGGAAGGA ATGACAGATG AGGAGGCCTT GGTGCCCTAT
    AGCAAGCCCA GTTTCCCATC TCCAGGTGGC CACAGCTCAT CAGGAACAGC TTCTTCTAAG
    GGATCCACTG GACCTAGGAA AACCGAGGTG TTGAGAGCAG GCCACCAGCG CAATGCCAGC
    GACCTTCTTG ACATAGGATA TATGGGCTCC AACAGTCAAG GACAGTTTAC AGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94472
    Clone ID Related Accession (Same CDS sequence) XM_017006998.1
    Accession Version XM_017006998.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product roundabout homolog 2 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC
    ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC
    ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC
    CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG
    AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA
    GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG
    TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA
    GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG
    GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT
    AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA
    AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCGCC CTGTTGCTCC CCCACAGTTT
    GTGGTTCGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT
    TTCCCAAACC AACCCCAGCA GCCCAACAGT AGATGCTCAG TGTCACCAAC TGGAGACCTC
    ACAATCACCA ACATTCAACG TTCCGACGCG GGTTACTACA TCTGCCAGGC TTTAACTGTG
    GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAGGTTACTG ATGTTTTGAC AGATAGACCT
    CCACCTATAA TTCTACAAGG CCCAGCCAAC CAAACGCTGG CAGTGGATGG TACAGCGTTA
    CTGAAATGTA AAGCCACTGG TGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT
    ACTTTTCCGG GTAGAGATCC AAGAGCAACA ATTCAAGAGC AAGGCACACT GCAGATTAAG
    AATTTACGGA TTTCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGAGAG
    ACTTCCTGGA GTGCAGTGCT GGATGTGACA GAGTCTGGAG CAACAATCAG TAAAAACTAT
    GATTTAAGTG ACCTGCCAGG GCCACCATCC AAACCGCAGG TCACTGATGT TACTAAGAAC
    AGTGTCACCT TGTCCTGGCA GCCAGGTACC CCTGGAACCC TTCCAGCAAG TGCATATATC
    ATTGAGGCTT TCAGCCAATC AGTGAGCAAC AGCTGGCAGA CCGTGGCAAA CCATGTAAAG
    ACCACCCTCT ATACTGTAAG AGGACTGCGG CCCAATACAA TCTACTTATT CATGGTCAGA
    GCGATCAACC CCCAAGGTCT CAGTGACCCA AGTCCCATGT CAGATCCTGT GCGCACACAA
    GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TGCAGAAAGA GCTAGGAGAT
    GTCCTTGTCC GTCTTCATAA TCCAGTTGTG CTGACTCCCA CCACGGTTCA GGTCACATGG
    ACGGTTGATC GCCAACCCCA GTTTATCCAA GGCTACCGAG TGATGTATCG TCAGACTTCA
    GGTCTGCAGG CGACATCTTC GTGGCAGAAT TTAGATGCCA AAGTCCCGAC TGAACGAAGT
    GCTGTCTTAG TCAACCTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTACG GCCATATTTT
    AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACGGTTC GTACTACTGA AGAAGCCCCA
    AGTGCCCCAC CACAGTCTGT CACTGTACTG ACAGTTGGAA GCTACAATAG CACAAGTATT
    AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CACCAGAATG GAATTATCCA AGAATACAAG
    ATCTGGTGTC TAGGAAATGA AACGCGATTC CATATCAACA AAACTGTGGA TGCAGCCATT
    CGGTCCGTAA TAATTGGTGG ATTATTCCCA GGTATTCAAT ACCGGGTAGA GGTTGCAGCT
    AGTACCAGTG CAGGGGTTGG AGTAAAGAGT GAGCCACAGC CAATAATAAT CGGGAGACGC
    AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTGGTG
    AAGCAACCAG CCTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT
    AGCATATGGT TGTATTGGCG AAGAAAGAAG AGGAAGGGAC TCAGTAATTA TGCTGTTACG
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GTCCAGGTCT TCTCAATGCT
    GGTGATCCCA GCTATCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGCTT GCCAGTAAAT
    AATAGCAACA GTGGCCCAAA TGAGATTGGA AATTTTGGCC GTGGAGATGT GCTGCCACCA
    GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGTAGC
    ATTGACTTCA CTACCAAAAC CAGTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA
    TATGCCACGA CACAGATCTT GCATTCCAAC AGCATACATG AATTGGCTGT CGATCTGCCT
    GATCCACAAT GGAAAAGCTC AATTCAGCAA AAAACAGATC TGATGGGATT TGGTTATTCT
    CTACCTGATC AGAACAAAGG TAACAATGCC TTACTTTACA TTCCTGACTA CCGATTGGCT
    GAGGGATTGT CTAATAGAAT GCCACACAAC CAGTCTCAGG ATTTCAGCAC CACCAGCTCT
    CACAACAGCT CAGAAAGGAG TGGCAGTCTC TCAGGTGGGA AAGGTGGAAA AAAGAAGAAA
    AATAAAAACT CTTCTAAACC ACAGAAAAAC AATGGATCCA CTTGGGCCAA TGTCCCTCTA
    CCTCCCCCCC CAGTCCAGCC CCTTCCTGGC ACGGAGCTGG AACACTATGC AGTGGAACAA
    CAAGAAAATG GCTATGACAG TGATAGCTGG TGCCCACCAT TGCCAGTACA AACTTACTTA
    CACCAAGGTC TGGAAGATGA ACTGGAAGAA GATGATGATA GGGTCCCAAC ACCTCCTGTT
    CGAGGCGTGG CTTCTTCTCC TGCTATCTCC TTTGGACAGC AGTCCACTGC AACTCTTACT
    CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAGGCTC ACCTGGATGA GTTGACAAGA
    GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAA TCAACCTCCA
    CAGCCTCCAG TTCCACCGTT AGGTTATGTG TCTGGAGCCT TGATTTCTGA TTTGGAAACG
    GATGTTGCAG ATGATGATGC CGACGACGAA GAGGAAGCTT TAGAAATCCC CAGGCCCCTG
    AGAGCACTGG ACCAGACTCC TGGATCCAGC ATGGACAATC TAGACAGCTC TGTGACAGGA
    AAAGCCTTTA CCTCCTCTCA AAGACCTCGA CCTACCAGCC CATTTTCTAC TGACAGTAAC
    ACCAGTGCAG CCCTGAGTCA AAGTCAGAGG CCTCGGCCCA CTAAAAAACA CAAGGGAGGG
    CGGATGGACC AACAACCAGC ATTGCCTCAT CGAAGGGAAG GAATGACAGA TGAGGAGGCC
    TTGGTGCCCT ATAGCAAGCC CAGTTTCCCA TCTCCAGGTG GCCACAGCTC ATCAGGAACA
    GCTTCTTCTA AGGGATCCAC TGGACCTAGG AAAACCGAGG TGTTGAGAGC AGGCCACCAG
    CGCAATGCCA GCGACCTTCT TGACATAGGA TATATGGGCT CCAACAGTCA AGGACAGTTT
    ACAGGTGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016862487.1
    CDS692..4966
    Translation

    Target ORF information:

    RefSeq Version XM_017006998.1
    Organism Homo sapiens(human)
    Definition Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017006998.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC
    ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC
    ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC
    CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG
    AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA
    GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG
    TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA
    GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG
    GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT
    AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA
    AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCGCC CTGTTGCTCC CCCACAGTTT
    GTGGTTCGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT
    TTCCCAAACC AACCCCAGCA GCCCAACAGT AGATGCTCAG TGTCACCAAC TGGAGACCTC
    ACAATCACCA ACATTCAACG TTCCGACGCG GGTTACTACA TCTGCCAGGC TTTAACTGTG
    GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAGGTTACTG ATGTTTTGAC AGATAGACCT
    CCACCTATAA TTCTACAAGG CCCAGCCAAC CAAACGCTGG CAGTGGATGG TACAGCGTTA
    CTGAAATGTA AAGCCACTGG TGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT
    ACTTTTCCGG GTAGAGATCC AAGAGCAACA ATTCAAGAGC AAGGCACACT GCAGATTAAG
    AATTTACGGA TTTCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGAGAG
    ACTTCCTGGA GTGCAGTGCT GGATGTGACA GAGTCTGGAG CAACAATCAG TAAAAACTAT
    GATTTAAGTG ACCTGCCAGG GCCACCATCC AAACCGCAGG TCACTGATGT TACTAAGAAC
    AGTGTCACCT TGTCCTGGCA GCCAGGTACC CCTGGAACCC TTCCAGCAAG TGCATATATC
    ATTGAGGCTT TCAGCCAATC AGTGAGCAAC AGCTGGCAGA CCGTGGCAAA CCATGTAAAG
    ACCACCCTCT ATACTGTAAG AGGACTGCGG CCCAATACAA TCTACTTATT CATGGTCAGA
    GCGATCAACC CCCAAGGTCT CAGTGACCCA AGTCCCATGT CAGATCCTGT GCGCACACAA
    GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TGCAGAAAGA GCTAGGAGAT
    GTCCTTGTCC GTCTTCATAA TCCAGTTGTG CTGACTCCCA CCACGGTTCA GGTCACATGG
    ACGGTTGATC GCCAACCCCA GTTTATCCAA GGCTACCGAG TGATGTATCG TCAGACTTCA
    GGTCTGCAGG CGACATCTTC GTGGCAGAAT TTAGATGCCA AAGTCCCGAC TGAACGAAGT
    GCTGTCTTAG TCAACCTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTACG GCCATATTTT
    AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACGGTTC GTACTACTGA AGAAGCCCCA
    AGTGCCCCAC CACAGTCTGT CACTGTACTG ACAGTTGGAA GCTACAATAG CACAAGTATT
    AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CACCAGAATG GAATTATCCA AGAATACAAG
    ATCTGGTGTC TAGGAAATGA AACGCGATTC CATATCAACA AAACTGTGGA TGCAGCCATT
    CGGTCCGTAA TAATTGGTGG ATTATTCCCA GGTATTCAAT ACCGGGTAGA GGTTGCAGCT
    AGTACCAGTG CAGGGGTTGG AGTAAAGAGT GAGCCACAGC CAATAATAAT CGGGAGACGC
    AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTGGTG
    AAGCAACCAG CCTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT
    AGCATATGGT TGTATTGGCG AAGAAAGAAG AGGAAGGGAC TCAGTAATTA TGCTGTTACG
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GTCCAGGTCT TCTCAATGCT
    GGTGATCCCA GCTATCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGCTT GCCAGTAAAT
    AATAGCAACA GTGGCCCAAA TGAGATTGGA AATTTTGGCC GTGGAGATGT GCTGCCACCA
    GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGTAGC
    ATTGACTTCA CTACCAAAAC CAGTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA
    TATGCCACGA CACAGATCTT GCATTCCAAC AGCATACATG AATTGGCTGT CGATCTGCCT
    GATCCACAAT GGAAAAGCTC AATTCAGCAA AAAACAGATC TGATGGGATT TGGTTATTCT
    CTACCTGATC AGAACAAAGG TAACAATGCC TTACTTTACA TTCCTGACTA CCGATTGGCT
    GAGGGATTGT CTAATAGAAT GCCACACAAC CAGTCTCAGG ATTTCAGCAC CACCAGCTCT
    CACAACAGCT CAGAAAGGAG TGGCAGTCTC TCAGGTGGGA AAGGTGGAAA AAAGAAGAAA
    AATAAAAACT CTTCTAAACC ACAGAAAAAC AATGGATCCA CTTGGGCCAA TGTCCCTCTA
    CCTCCCCCCC CAGTCCAGCC CCTTCCTGGC ACGGAGCTGG AACACTATGC AGTGGAACAA
    CAAGAAAATG GCTATGACAG TGATAGCTGG TGCCCACCAT TGCCAGTACA AACTTACTTA
    CACCAAGGTC TGGAAGATGA ACTGGAAGAA GATGATGATA GGGTCCCAAC ACCTCCTGTT
    CGAGGCGTGG CTTCTTCTCC TGCTATCTCC TTTGGACAGC AGTCCACTGC AACTCTTACT
    CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAGGCTC ACCTGGATGA GTTGACAAGA
    GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAA TCAACCTCCA
    CAGCCTCCAG TTCCACCGTT AGGTTATGTG TCTGGAGCCT TGATTTCTGA TTTGGAAACG
    GATGTTGCAG ATGATGATGC CGACGACGAA GAGGAAGCTT TAGAAATCCC CAGGCCCCTG
    AGAGCACTGG ACCAGACTCC TGGATCCAGC ATGGACAATC TAGACAGCTC TGTGACAGGA
    AAAGCCTTTA CCTCCTCTCA AAGACCTCGA CCTACCAGCC CATTTTCTAC TGACAGTAAC
    ACCAGTGCAG CCCTGAGTCA AAGTCAGAGG CCTCGGCCCA CTAAAAAACA CAAGGGAGGG
    CGGATGGACC AACAACCAGC ATTGCCTCAT CGAAGGGAAG GAATGACAGA TGAGGAGGCC
    TTGGTGCCCT ATAGCAAGCC CAGTTTCCCA TCTCCAGGTG GCCACAGCTC ATCAGGAACA
    GCTTCTTCTA AGGGATCCAC TGGACCTAGG AAAACCGAGG TGTTGAGAGC AGGCCACCAG
    CGCAATGCCA GCGACCTTCT TGACATAGGA TATATGGGCT CCAACAGTCA AGGACAGTTT
    ACAGGTGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94473
    Clone ID Related Accession (Same CDS sequence) XM_017006999.1
    Accession Version XM_017006999.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4269bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product roundabout homolog 2 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC
    ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC
    ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC
    CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG
    AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA
    GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG
    TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA
    GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG
    GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT
    AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA
    AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCGCC CTGTTGCTCC CCCACAGTTT
    GTGGTTCGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT
    TTCCCAAACC AACCCCAGCA GCCCAACAGT AGATGCTCAG TGTCACCAAC TGGAGACCTC
    ACAATCACCA ACATTCAACG TTCCGACGCG GGTTACTACA TCTGCCAGGC TTTAACTGTG
    GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAGGTTACTG ATGTTTTGAC AGATAGACCT
    CCACCTATAA TTCTACAAGG CCCAGCCAAC CAAACGCTGG CAGTGGATGG TACAGCGTTA
    CTGAAATGTA AAGCCACTGG TGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT
    ACTTTTCCGG GTAGAGATCC AAGAGCAACA ATTCAAGAGC AAGGCACACT GCAGATTAAG
    AATTTACGGA TTTCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGAGAG
    ACTTCCTGGA GTGCAGTGCT GGATGTGACA GAGTCTGGAG CAACAATCAG TAAAAACTAT
    GATTTAAGTG ACCTGCCAGG GCCACCATCC AAACCGCAGG TCACTGATGT TACTAAGAAC
    AGTGTCACCT TGTCCTGGCA GCCAGGTACC CCTGGAACCC TTCCAGCAAG TGCATATATC
    ATTGAGGCTT TCAGCCAATC AGTGAGCAAC AGCTGGCAGA CCGTGGCAAA CCATGTAAAG
    ACCACCCTCT ATACTGTAAG AGGACTGCGG CCCAATACAA TCTACTTATT CATGGTCAGA
    GCGATCAACC CCCAAGGTCT CAGTGACCCA AGTCCCATGT CAGATCCTGT GCGCACACAA
    GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TGCAGAAAGA GCTAGGAGAT
    GTCCTTGTCC GTCTTCATAA TCCAGTTGTG CTGACTCCCA CCACGGTTCA GGTCACATGG
    ACGGTTGATC GCCAACCCCA GTTTATCCAA GGCTACCGAG TGATGTATCG TCAGACTTCA
    GGTCTGCAGG CGACATCTTC GTGGCAGAAT TTAGATGCCA AAGTCCCGAC TGAACGAAGT
    GCTGTCTTAG TCAACCTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTACG GCCATATTTT
    AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACGGTTC GTACTACTGA AGAAGCCCCA
    AGTGCCCCAC CACAGTCTGT CACTGTACTG ACAGTTGGAA GCTACAATAG CACAAGTATT
    AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CACCAGAATG GAATTATCCA AGAATACAAG
    ATCTGGTGTC TAGGAAATGA AACGCGATTC CATATCAACA AAACTGTGGA TGCAGCCATT
    CGGTCCGTAA TAATTGGTGG ATTATTCCCA GGTATTCAAT ACCGGGTAGA GGTTGCAGCT
    AGTACCAGTG CAGGGGTTGG AGTAAAGAGT GAGCCACAGC CAATAATAAT CGGGAGACGC
    AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTGGTG
    AAGCAACCAG CCTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT
    AGCATATGGT TGTATTGGCG AAGAAAGAAG AGGAAGGGAC TCAGTAATTA TGCTGTTACG
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GTCCAGGTCT TCTCAATGCT
    GGTGATCCCA GCTATCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGCTT GCCAGTAAAT
    AATAGCAACA GTGGCCCAAA TGAGATTGGA AATTTTGGCC GTGGAGATGT GCTGCCACCA
    GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGTAGC
    ATTGACTTCA CTACCAAAAC CAGTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA
    TATGCCACGA CACAGATCTT GCATTCCAAC AGCATACATG AATTGGCTGT CGATCTGCCT
    GATCCACAAT GGAAAAGCTC AATTCAGCAA AAAACAGATC TGATGGGATT TGGTTATTCT
    CTACCTGATC AGAACAAAGG TAACAATGCC TTACTTTACA TTCCTGACTA CCGATTGGCT
    GAGGGATTGT CTAATAGAAT GCCACACAAC CAGTCTCAGG ATTTCAGCAC CACCAGCTCT
    CACAACAGCT CAGAAAGGAG TGGCAGTCTC TCAGGTGGGA AAGGTGGAAA AAAGAAGAAA
    AATAAAAACT CTTCTAAACC ACAGAAAAAC AATGGATCCA CTTGGGCCAA TGTCCCTCTA
    CCTCCCCCCC CAGTCCAGCC CCTTCCTGGC ACGGAGCTGG AACACTATGC AGTGGAACAA
    CAAGAAAATG GCTATGACAG TGATAGCTGG TGCCCACCAT TGCCAGTACA AACTTACTTA
    CACCAAGGTC TGGAAGATGA ACTGGAAGAA GATGATGATA GGGTCCCAAC ACCTCCTGTT
    CGAGGCGTGG CTTCTTCTCC TGCTATCTCC TTTGGACAGC AGTCCACTGC AACTCTTACT
    CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAGGCTC ACCTGGATGA GTTGACAAGA
    GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAA TCAACCTCCA
    CAGCCTCCAG TTCCACCGTT AGGTTATGTG TCTGGAGCCT TGATTTCTGA TTTGGAAACG
    GATGTTGCAG ATGATGATGC CGACGACGAA GAGGAAGCTT TAGAAATCCC CAGGCCCCTG
    AGAGCACTGG ACCAGACTCC TGGATCCAGC ATGGACAATC TAGACAGCTC TGTGACAGGA
    AAAGCCTTTA CCTCCTCTCA AAGACCTCGA CCTACCAGCC CATTTTCTAC TGACAGTAAC
    ACCAGTGCAG CCCTGAGTCA AAGTCAGAGG CCTCGGCCCA CTAAAAAACA CAAGGGAGGG
    CGGATGGACC AACAACCAGC ATTGCCTCAT CGAAGGGAAG GAATGACAGA TGAGGAGGCC
    TTGGTGCCCT ATAGCAAGCC CAGTTTCCCA TCTCCAGGTG GCCACAGCTC ATCAGGAACA
    GCTTCTTCTA AGGGATCCAC TGGACCTAGG AAAACCGAGG TGTTGAGAGC AGGCCACCAG
    CGCAATGCCA GCGACCTTCT TGACATAGGA TATATGGGCT CCAACAGTCA AGGACAGTTT
    ACAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016862488.1
    CDS692..4960
    Translation

    Target ORF information:

    RefSeq Version XM_017006999.1
    Organism Homo sapiens(human)
    Definition Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017006999.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC
    ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC
    ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC
    CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG
    AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA
    GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG
    TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA
    GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG
    GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT
    AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA
    AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCGCC CTGTTGCTCC CCCACAGTTT
    GTGGTTCGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT
    TTCCCAAACC AACCCCAGCA GCCCAACAGT AGATGCTCAG TGTCACCAAC TGGAGACCTC
    ACAATCACCA ACATTCAACG TTCCGACGCG GGTTACTACA TCTGCCAGGC TTTAACTGTG
    GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAGGTTACTG ATGTTTTGAC AGATAGACCT
    CCACCTATAA TTCTACAAGG CCCAGCCAAC CAAACGCTGG CAGTGGATGG TACAGCGTTA
    CTGAAATGTA AAGCCACTGG TGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT
    ACTTTTCCGG GTAGAGATCC AAGAGCAACA ATTCAAGAGC AAGGCACACT GCAGATTAAG
    AATTTACGGA TTTCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGAGAG
    ACTTCCTGGA GTGCAGTGCT GGATGTGACA GAGTCTGGAG CAACAATCAG TAAAAACTAT
    GATTTAAGTG ACCTGCCAGG GCCACCATCC AAACCGCAGG TCACTGATGT TACTAAGAAC
    AGTGTCACCT TGTCCTGGCA GCCAGGTACC CCTGGAACCC TTCCAGCAAG TGCATATATC
    ATTGAGGCTT TCAGCCAATC AGTGAGCAAC AGCTGGCAGA CCGTGGCAAA CCATGTAAAG
    ACCACCCTCT ATACTGTAAG AGGACTGCGG CCCAATACAA TCTACTTATT CATGGTCAGA
    GCGATCAACC CCCAAGGTCT CAGTGACCCA AGTCCCATGT CAGATCCTGT GCGCACACAA
    GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TGCAGAAAGA GCTAGGAGAT
    GTCCTTGTCC GTCTTCATAA TCCAGTTGTG CTGACTCCCA CCACGGTTCA GGTCACATGG
    ACGGTTGATC GCCAACCCCA GTTTATCCAA GGCTACCGAG TGATGTATCG TCAGACTTCA
    GGTCTGCAGG CGACATCTTC GTGGCAGAAT TTAGATGCCA AAGTCCCGAC TGAACGAAGT
    GCTGTCTTAG TCAACCTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTACG GCCATATTTT
    AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACGGTTC GTACTACTGA AGAAGCCCCA
    AGTGCCCCAC CACAGTCTGT CACTGTACTG ACAGTTGGAA GCTACAATAG CACAAGTATT
    AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CACCAGAATG GAATTATCCA AGAATACAAG
    ATCTGGTGTC TAGGAAATGA AACGCGATTC CATATCAACA AAACTGTGGA TGCAGCCATT
    CGGTCCGTAA TAATTGGTGG ATTATTCCCA GGTATTCAAT ACCGGGTAGA GGTTGCAGCT
    AGTACCAGTG CAGGGGTTGG AGTAAAGAGT GAGCCACAGC CAATAATAAT CGGGAGACGC
    AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTGGTG
    AAGCAACCAG CCTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT
    AGCATATGGT TGTATTGGCG AAGAAAGAAG AGGAAGGGAC TCAGTAATTA TGCTGTTACG
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GTCCAGGTCT TCTCAATGCT
    GGTGATCCCA GCTATCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGCTT GCCAGTAAAT
    AATAGCAACA GTGGCCCAAA TGAGATTGGA AATTTTGGCC GTGGAGATGT GCTGCCACCA
    GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGTAGC
    ATTGACTTCA CTACCAAAAC CAGTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA
    TATGCCACGA CACAGATCTT GCATTCCAAC AGCATACATG AATTGGCTGT CGATCTGCCT
    GATCCACAAT GGAAAAGCTC AATTCAGCAA AAAACAGATC TGATGGGATT TGGTTATTCT
    CTACCTGATC AGAACAAAGG TAACAATGCC TTACTTTACA TTCCTGACTA CCGATTGGCT
    GAGGGATTGT CTAATAGAAT GCCACACAAC CAGTCTCAGG ATTTCAGCAC CACCAGCTCT
    CACAACAGCT CAGAAAGGAG TGGCAGTCTC TCAGGTGGGA AAGGTGGAAA AAAGAAGAAA
    AATAAAAACT CTTCTAAACC ACAGAAAAAC AATGGATCCA CTTGGGCCAA TGTCCCTCTA
    CCTCCCCCCC CAGTCCAGCC CCTTCCTGGC ACGGAGCTGG AACACTATGC AGTGGAACAA
    CAAGAAAATG GCTATGACAG TGATAGCTGG TGCCCACCAT TGCCAGTACA AACTTACTTA
    CACCAAGGTC TGGAAGATGA ACTGGAAGAA GATGATGATA GGGTCCCAAC ACCTCCTGTT
    CGAGGCGTGG CTTCTTCTCC TGCTATCTCC TTTGGACAGC AGTCCACTGC AACTCTTACT
    CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAGGCTC ACCTGGATGA GTTGACAAGA
    GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAA TCAACCTCCA
    CAGCCTCCAG TTCCACCGTT AGGTTATGTG TCTGGAGCCT TGATTTCTGA TTTGGAAACG
    GATGTTGCAG ATGATGATGC CGACGACGAA GAGGAAGCTT TAGAAATCCC CAGGCCCCTG
    AGAGCACTGG ACCAGACTCC TGGATCCAGC ATGGACAATC TAGACAGCTC TGTGACAGGA
    AAAGCCTTTA CCTCCTCTCA AAGACCTCGA CCTACCAGCC CATTTTCTAC TGACAGTAAC
    ACCAGTGCAG CCCTGAGTCA AAGTCAGAGG CCTCGGCCCA CTAAAAAACA CAAGGGAGGG
    CGGATGGACC AACAACCAGC ATTGCCTCAT CGAAGGGAAG GAATGACAGA TGAGGAGGCC
    TTGGTGCCCT ATAGCAAGCC CAGTTTCCCA TCTCCAGGTG GCCACAGCTC ATCAGGAACA
    GCTTCTTCTA AGGGATCCAC TGGACCTAGG AAAACCGAGG TGTTGAGAGC AGGCCACCAG
    CGCAATGCCA GCGACCTTCT TGACATAGGA TATATGGGCT CCAACAGTCA AGGACAGTTT
    ACAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94474
    Clone ID Related Accession (Same CDS sequence) XM_017007000.1
    Accession Version XM_017007000.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4263bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product roundabout homolog 2 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC
    ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC
    ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC
    CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG
    AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA
    GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG
    TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA
    GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG
    GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT
    AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA
    AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCCCC CACAGTTTGT GGTTCGGCCA
    AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC
    CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA
    CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC
    ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT
    TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT
    CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA
    GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT
    AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT
    TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT
    GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC
    CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG
    TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC
    AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT
    ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC
    CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA
    CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT
    CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC
    CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG
    ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC
    AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA
    GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA
    CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG
    GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA
    GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA
    ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA
    GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC
    ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC
    TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG
    TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA
    GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC
    TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT
    GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA
    GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT
    ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA
    CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG
    AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG
    AACAAAGGTA ACAATGCCTT ACTTTACATT CCTGACTACC GATTGGCTGA GGGATTGTCT
    AATAGAATGC CACACAACCA GTCTCAGGAT TTCAGCACCA CCAGCTCTCA CAACAGCTCA
    GAAAGGAGTG GCAGTCTCTC AGGTGGGAAA GGTGGAAAAA AGAAGAAAAA TAAAAACTCT
    TCTAAACCAC AGAAAAACAA TGGATCCACT TGGGCCAATG TCCCTCTACC TCCCCCCCCA
    GTCCAGCCCC TTCCTGGCAC GGAGCTGGAA CACTATGCAG TGGAACAACA AGAAAATGGC
    TATGACAGTG ATAGCTGGTG CCCACCATTG CCAGTACAAA CTTACTTACA CCAAGGTCTG
    GAAGATGAAC TGGAAGAAGA TGATGATAGG GTCCCAACAC CTCCTGTTCG AGGCGTGGCT
    TCTTCTCCTG CTATCTCCTT TGGACAGCAG TCCACTGCAA CTCTTACTCC ATCCCCACGG
    GAAGAGATGC AACCCATGCT GCAGGCTCAC CTGGATGAGT TGACAAGAGC CTATCAGTTT
    GATATAGCAA AACAAACATG GCACATTCAA AGCAATAATC AACCTCCACA GCCTCCAGTT
    CCACCGTTAG GTTATGTGTC TGGAGCCTTG ATTTCTGATT TGGAAACGGA TGTTGCAGAT
    GATGATGCCG ACGACGAAGA GGAAGCTTTA GAAATCCCCA GGCCCCTGAG AGCACTGGAC
    CAGACTCCTG GATCCAGCAT GGACAATCTA GACAGCTCTG TGACAGGAAA AGCCTTTACC
    TCCTCTCAAA GACCTCGACC TACCAGCCCA TTTTCTACTG ACAGTAACAC CAGTGCAGCC
    CTGAGTCAAA GTCAGAGGCC TCGGCCCACT AAAAAACACA AGGGAGGGCG GATGGACCAA
    CAACCAGCAT TGCCTCATCG AAGGGAAGGA ATGACAGATG AGGAGGCCTT GGTGCCCTAT
    AGCAAGCCCA GTTTCCCATC TCCAGGTGGC CACAGCTCAT CAGGAACAGC TTCTTCTAAG
    GGATCCACTG GACCTAGGAA AACCGAGGTG TTGAGAGCAG GCCACCAGCG CAATGCCAGC
    GACCTTCTTG ACATAGGATA TATGGGCTCC AACAGTCAAG GACAGTTTAC AGGTGAATTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016862489.1
    CDS692..4954
    Translation

    Target ORF information:

    RefSeq Version XM_017007000.1
    Organism Homo sapiens(human)
    Definition Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017007000.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC
    ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC
    ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC
    CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG
    AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA
    GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG
    TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA
    GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG
    GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT
    AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA
    AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCCCC CACAGTTTGT GGTTCGGCCA
    AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC
    CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA
    CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC
    ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT
    TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT
    CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA
    GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT
    AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT
    TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT
    GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC
    CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG
    TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC
    AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT
    ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC
    CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA
    CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT
    CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC
    CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG
    ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC
    AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA
    GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA
    CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG
    GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA
    GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA
    ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA
    GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC
    ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC
    TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG
    TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA
    GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC
    TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT
    GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA
    GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT
    ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA
    CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG
    AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG
    AACAAAGGTA ACAATGCCTT ACTTTACATT CCTGACTACC GATTGGCTGA GGGATTGTCT
    AATAGAATGC CACACAACCA GTCTCAGGAT TTCAGCACCA CCAGCTCTCA CAACAGCTCA
    GAAAGGAGTG GCAGTCTCTC AGGTGGGAAA GGTGGAAAAA AGAAGAAAAA TAAAAACTCT
    TCTAAACCAC AGAAAAACAA TGGATCCACT TGGGCCAATG TCCCTCTACC TCCCCCCCCA
    GTCCAGCCCC TTCCTGGCAC GGAGCTGGAA CACTATGCAG TGGAACAACA AGAAAATGGC
    TATGACAGTG ATAGCTGGTG CCCACCATTG CCAGTACAAA CTTACTTACA CCAAGGTCTG
    GAAGATGAAC TGGAAGAAGA TGATGATAGG GTCCCAACAC CTCCTGTTCG AGGCGTGGCT
    TCTTCTCCTG CTATCTCCTT TGGACAGCAG TCCACTGCAA CTCTTACTCC ATCCCCACGG
    GAAGAGATGC AACCCATGCT GCAGGCTCAC CTGGATGAGT TGACAAGAGC CTATCAGTTT
    GATATAGCAA AACAAACATG GCACATTCAA AGCAATAATC AACCTCCACA GCCTCCAGTT
    CCACCGTTAG GTTATGTGTC TGGAGCCTTG ATTTCTGATT TGGAAACGGA TGTTGCAGAT
    GATGATGCCG ACGACGAAGA GGAAGCTTTA GAAATCCCCA GGCCCCTGAG AGCACTGGAC
    CAGACTCCTG GATCCAGCAT GGACAATCTA GACAGCTCTG TGACAGGAAA AGCCTTTACC
    TCCTCTCAAA GACCTCGACC TACCAGCCCA TTTTCTACTG ACAGTAACAC CAGTGCAGCC
    CTGAGTCAAA GTCAGAGGCC TCGGCCCACT AAAAAACACA AGGGAGGGCG GATGGACCAA
    CAACCAGCAT TGCCTCATCG AAGGGAAGGA ATGACAGATG AGGAGGCCTT GGTGCCCTAT
    AGCAAGCCCA GTTTCCCATC TCCAGGTGGC CACAGCTCAT CAGGAACAGC TTCTTCTAAG
    GGATCCACTG GACCTAGGAA AACCGAGGTG TTGAGAGCAG GCCACCAGCG CAATGCCAGC
    GACCTTCTTG ACATAGGATA TATGGGCTCC AACAGTCAAG GACAGTTTAC AGGTGAATTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94475
    Clone ID Related Accession (Same CDS sequence) XM_017007001.1
    Accession Version XM_017007001.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4212bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product roundabout homolog 2 isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
    CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT
    TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT
    CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC
    GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA
    AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC
    CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG
    TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA
    CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT
    CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC
    CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT
    GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT
    ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT
    AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC
    CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC
    ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG
    CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG
    GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT
    CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT
    GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG
    CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA
    GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG
    GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC
    GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT
    GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT
    CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG
    CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC
    GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT
    GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGGTG GGAAAGGTGG AAAAAAGAAG
    AAAAATAAAA ACTCTTCTAA ACCACAGAAA AACAATGGAT CCACTTGGGC CAATGTCCCT
    CTACCTCCCC CCCCAGTCCA GCCCCTTCCT GGCACGGAGC TGGAACACTA TGCAGTGGAA
    CAACAAGAAA ATGGCTATGA CAGTGATAGC TGGTGCCCAC CATTGCCAGT ACAAACTTAC
    TTACACCAAG GTCTGGAAGA TGAACTGGAA GAAGATGATG ATAGGGTCCC AACACCTCCT
    GTTCGAGGCG TGGCTTCTTC TCCTGCTATC TCCTTTGGAC AGCAGTCCAC TGCAACTCTT
    ACTCCATCCC CACGGGAAGA GATGCAACCC ATGCTGCAGG CTCACCTGGA TGAGTTGACA
    AGAGCCTATC AGTTTGATAT AGCAAAACAA ACATGGCACA TTCAAAGCAA TAATCAACCT
    CCACAGCCTC CAGTTCCACC GTTAGGTTAT GTGTCTGGAG CCTTGATTTC TGATTTGGAA
    ACGGATGTTG CAGATGATGA TGCCGACGAC GAAGAGGAAG CTTTAGAAAT CCCCAGGCCC
    CTGAGAGCAC TGGACCAGAC TCCTGGATCC AGCATGGACA ATCTAGACAG CTCTGTGACA
    GGAAAAGCCT TTACCTCCTC TCAAAGACCT CGACCTACCA GCCCATTTTC TACTGACAGT
    AACACCAGTG CAGCCCTGAG TCAAAGTCAG AGGCCTCGGC CCACTAAAAA ACACAAGGGA
    GGGCGGATGG ACCAACAACC AGCATTGCCT CATCGAAGGG AAGGAATGAC AGATGAGGAG
    GCCTTGGTGC CCTATAGCAA GCCCAGTTTC CCATCTCCAG GTGGCCACAG CTCATCAGGA
    ACAGCTTCTT CTAAGGGATC CACTGGACCT AGGAAAACCG AGGTGTTGAG AGCAGGCCAC
    CAGCGCAATG CCAGCGACCT TCTTGACATA GGATATATGG GCTCCAACAG TCAAGGACAG
    TTTACAGAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016862490.1
    CDS400..4611
    Translation

    Target ORF information:

    RefSeq Version XM_017007001.1
    Organism Homo sapiens(human)
    Definition Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017007001.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
    CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT
    TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT
    CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC
    GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA
    AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC
    CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG
    TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA
    CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT
    CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC
    CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT
    GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT
    ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT
    AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC
    CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC
    ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG
    CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG
    GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT
    CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT
    GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG
    CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA
    GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG
    GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC
    GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT
    GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT
    CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG
    CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC
    GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT
    GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGGTG GGAAAGGTGG AAAAAAGAAG
    AAAAATAAAA ACTCTTCTAA ACCACAGAAA AACAATGGAT CCACTTGGGC CAATGTCCCT
    CTACCTCCCC CCCCAGTCCA GCCCCTTCCT GGCACGGAGC TGGAACACTA TGCAGTGGAA
    CAACAAGAAA ATGGCTATGA CAGTGATAGC TGGTGCCCAC CATTGCCAGT ACAAACTTAC
    TTACACCAAG GTCTGGAAGA TGAACTGGAA GAAGATGATG ATAGGGTCCC AACACCTCCT
    GTTCGAGGCG TGGCTTCTTC TCCTGCTATC TCCTTTGGAC AGCAGTCCAC TGCAACTCTT
    ACTCCATCCC CACGGGAAGA GATGCAACCC ATGCTGCAGG CTCACCTGGA TGAGTTGACA
    AGAGCCTATC AGTTTGATAT AGCAAAACAA ACATGGCACA TTCAAAGCAA TAATCAACCT
    CCACAGCCTC CAGTTCCACC GTTAGGTTAT GTGTCTGGAG CCTTGATTTC TGATTTGGAA
    ACGGATGTTG CAGATGATGA TGCCGACGAC GAAGAGGAAG CTTTAGAAAT CCCCAGGCCC
    CTGAGAGCAC TGGACCAGAC TCCTGGATCC AGCATGGACA ATCTAGACAG CTCTGTGACA
    GGAAAAGCCT TTACCTCCTC TCAAAGACCT CGACCTACCA GCCCATTTTC TACTGACAGT
    AACACCAGTG CAGCCCTGAG TCAAAGTCAG AGGCCTCGGC CCACTAAAAA ACACAAGGGA
    GGGCGGATGG ACCAACAACC AGCATTGCCT CATCGAAGGG AAGGAATGAC AGATGAGGAG
    GCCTTGGTGC CCTATAGCAA GCCCAGTTTC CCATCTCCAG GTGGCCACAG CTCATCAGGA
    ACAGCTTCTT CTAAGGGATC CACTGGACCT AGGAAAACCG AGGTGTTGAG AGCAGGCCAC
    CAGCGCAATG CCAGCGACCT TCTTGACATA GGATATATGG GCTCCAACAG TCAAGGACAG
    TTTACAGAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94476
    Clone ID Related Accession (Same CDS sequence) XM_017007002.1
    Accession Version XM_017007002.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4209bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product roundabout homolog 2 isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
    CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT
    TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT
    CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC
    GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA
    AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC
    CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG
    TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA
    CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT
    CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC
    CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT
    GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT
    ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT
    AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC
    CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC
    ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG
    CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG
    GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT
    CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT
    GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG
    CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA
    GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG
    GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC
    GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT
    GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT
    CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG
    CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC
    GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT
    GGTTATTCTC TACCTGATCA GAACAAAGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA
    AACTCTTCTA AACCACAGAA AAACAATGGA TCCACTTGGG CCAATGTCCC TCTACCTCCC
    CCCCCAGTCC AGCCCCTTCC TGGCACGGAG CTGGAACACT ATGCAGTGGA ACAACAAGAA
    AATGGCTATG ACAGTGATAG CTGGTGCCCA CCATTGCCAG TACAAACTTA CTTACACCAA
    GGTCTGGAAG ATGAACTGGA AGAAGATGAT GATAGGGTCC CAACACCTCC TGTTCGAGGC
    GTGGCTTCTT CTCCTGCTAT CTCCTTTGGA CAGCAGTCCA CTGCAACTCT TACTCCATCC
    CCACGGGAAG AGATGCAACC CATGCTGCAG GCTCACCTGG ATGAGTTGAC AAGAGCCTAT
    CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATAATCAACC TCCACAGCCT
    CCAGTTCCAC CGTTAGGTTA TGTGTCTGGA GCCTTGATTT CTGATTTGGA AACGGATGTT
    GCAGATGATG ATGCCGACGA CGAAGAGGAA GCTTTAGAAA TCCCCAGGCC CCTGAGAGCA
    CTGGACCAGA CTCCTGGATC CAGCATGGAC AATCTAGACA GCTCTGTGAC AGGAAAAGCC
    TTTACCTCCT CTCAAAGACC TCGACCTACC AGCCCATTTT CTACTGACAG TAACACCAGT
    GCAGCCCTGA GTCAAAGTCA GAGGCCTCGG CCCACTAAAA AACACAAGGG AGGGCGGATG
    GACCAACAAC CAGCATTGCC TCATCGAAGG GAAGGAATGA CAGATGAGGA GGCCTTGGTG
    CCCTATAGCA AGCCCAGTTT CCCATCTCCA GGTGGCCACA GCTCATCAGG AACAGCTTCT
    TCTAAGGGAT CCACTGGACC TAGGAAAACC GAGGTGTTGA GAGCAGGCCA CCAGCGCAAT
    GCCAGCGACC TTCTTGACAT AGGATATATG GGCTCCAACA GTCAAGGACA GTTTACAGGT
    GAATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016862491.1
    CDS400..4608
    Translation

    Target ORF information:

    RefSeq Version XM_017007002.1
    Organism Homo sapiens(human)
    Definition Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017007002.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
    CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT
    TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT
    CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC
    GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA
    AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC
    CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG
    TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA
    CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT
    CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC
    CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT
    GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT
    ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT
    AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC
    CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC
    ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG
    CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG
    GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT
    CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT
    GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG
    CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA
    GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG
    GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC
    GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT
    GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT
    CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG
    CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC
    GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT
    GGTTATTCTC TACCTGATCA GAACAAAGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA
    AACTCTTCTA AACCACAGAA AAACAATGGA TCCACTTGGG CCAATGTCCC TCTACCTCCC
    CCCCCAGTCC AGCCCCTTCC TGGCACGGAG CTGGAACACT ATGCAGTGGA ACAACAAGAA
    AATGGCTATG ACAGTGATAG CTGGTGCCCA CCATTGCCAG TACAAACTTA CTTACACCAA
    GGTCTGGAAG ATGAACTGGA AGAAGATGAT GATAGGGTCC CAACACCTCC TGTTCGAGGC
    GTGGCTTCTT CTCCTGCTAT CTCCTTTGGA CAGCAGTCCA CTGCAACTCT TACTCCATCC
    CCACGGGAAG AGATGCAACC CATGCTGCAG GCTCACCTGG ATGAGTTGAC AAGAGCCTAT
    CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATAATCAACC TCCACAGCCT
    CCAGTTCCAC CGTTAGGTTA TGTGTCTGGA GCCTTGATTT CTGATTTGGA AACGGATGTT
    GCAGATGATG ATGCCGACGA CGAAGAGGAA GCTTTAGAAA TCCCCAGGCC CCTGAGAGCA
    CTGGACCAGA CTCCTGGATC CAGCATGGAC AATCTAGACA GCTCTGTGAC AGGAAAAGCC
    TTTACCTCCT CTCAAAGACC TCGACCTACC AGCCCATTTT CTACTGACAG TAACACCAGT
    GCAGCCCTGA GTCAAAGTCA GAGGCCTCGG CCCACTAAAA AACACAAGGG AGGGCGGATG
    GACCAACAAC CAGCATTGCC TCATCGAAGG GAAGGAATGA CAGATGAGGA GGCCTTGGTG
    CCCTATAGCA AGCCCAGTTT CCCATCTCCA GGTGGCCACA GCTCATCAGG AACAGCTTCT
    TCTAAGGGAT CCACTGGACC TAGGAAAACC GAGGTGTTGA GAGCAGGCCA CCAGCGCAAT
    GCCAGCGACC TTCTTGACAT AGGATATATG GGCTCCAACA GTCAAGGACA GTTTACAGGT
    GAATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94477
    Clone ID Related Accession (Same CDS sequence) XM_017007003.1
    Accession Version XM_017007003.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4206bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product roundabout homolog 2 isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
    CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT
    TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT
    CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC
    GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA
    AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC
    CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG
    TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA
    CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT
    CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCCCCC ACAGTTTGTG
    GTTCGGCCAA GAGATCAGAT TGTTGCTCAA GGTCGAACAG TGACATTTCC CTGTGAAACT
    AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAGAAAGAAG GCAGCCAGAA CCTACTTTTC
    CCAAACCAAC CCCAGCAGCC CAACAGTAGA TGCTCAGTGT CACCAACTGG AGACCTCACA
    ATCACCAACA TTCAACGTTC CGACGCGGGT TACTACATCT GCCAGGCTTT AACTGTGGCA
    GGAAGCATTT TAGCAAAAGC TCAACTGGAG GTTACTGATG TTTTGACAGA TAGACCTCCA
    CCTATAATTC TACAAGGCCC AGCCAACCAA ACGCTGGCAG TGGATGGTAC AGCGTTACTG
    AAATGTAAAG CCACTGGTGA TCCTCTTCCT GTAATTAGCT GGTTAAAGGA GGGATTTACT
    TTTCCGGGTA GAGATCCAAG AGCAACAATT CAAGAGCAAG GCACACTGCA GATTAAGAAT
    TTACGGATTT CTGATACTGG CACTTATACT TGTGTGGCTA CAAGTTCAAG TGGAGAGACT
    TCCTGGAGTG CAGTGCTGGA TGTGACAGAG TCTGGAGCAA CAATCAGTAA AAACTATGAT
    TTAAGTGACC TGCCAGGGCC ACCATCCAAA CCGCAGGTCA CTGATGTTAC TAAGAACAGT
    GTCACCTTGT CCTGGCAGCC AGGTACCCCT GGAACCCTTC CAGCAAGTGC ATATATCATT
    GAGGCTTTCA GCCAATCAGT GAGCAACAGC TGGCAGACCG TGGCAAACCA TGTAAAGACC
    ACCCTCTATA CTGTAAGAGG ACTGCGGCCC AATACAATCT ACTTATTCAT GGTCAGAGCG
    ATCAACCCCC AAGGTCTCAG TGACCCAAGT CCCATGTCAG ATCCTGTGCG CACACAAGAT
    ATCAGCCCAC CAGCACAAGG AGTGGACCAC AGGCAAGTGC AGAAAGAGCT AGGAGATGTC
    CTTGTCCGTC TTCATAATCC AGTTGTGCTG ACTCCCACCA CGGTTCAGGT CACATGGACG
    GTTGATCGCC AACCCCAGTT TATCCAAGGC TACCGAGTGA TGTATCGTCA GACTTCAGGT
    CTGCAGGCGA CATCTTCGTG GCAGAATTTA GATGCCAAAG TCCCGACTGA ACGAAGTGCT
    GTCTTAGTCA ACCTGAAAAA GGGGGTGACT TATGAAATTA AAGTACGGCC ATATTTTAAT
    GAGTTCCAAG GAATGGATAG TGAATCTAAA ACGGTTCGTA CTACTGAAGA AGCCCCAAGT
    GCCCCACCAC AGTCTGTCAC TGTACTGACA GTTGGAAGCT ACAATAGCAC AAGTATTAGT
    GTTTCCTGGG ATCCTCCTCC TCCAGATCAC CAGAATGGAA TTATCCAAGA ATACAAGATC
    TGGTGTCTAG GAAATGAAAC GCGATTCCAT ATCAACAAAA CTGTGGATGC AGCCATTCGG
    TCCGTAATAA TTGGTGGATT ATTCCCAGGT ATTCAATACC GGGTAGAGGT TGCAGCTAGT
    ACCAGTGCAG GGGTTGGAGT AAAGAGTGAG CCACAGCCAA TAATAATCGG GAGACGCAAT
    GAAGTTGTCA TTACTGAAAA CAATAACAGC ATAACTGAGC AAATCACTGA TGTGGTGAAG
    CAACCAGCCT TTATAGCTGG TATTGGTGGT GCCTGCTGGG TAATTCTGAT GGGTTTTAGC
    ATATGGTTGT ATTGGCGAAG AAAGAAGAGG AAGGGACTCA GTAATTATGC TGTTACGTTT
    CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGAAGCCGTC CAGGTCTTCT CAATGCTGGT
    GATCCCAGCT ATCCATGGCT TGCTGATTCT TGGCCAGCCA CGAGCTTGCC AGTAAATAAT
    AGCAACAGTG GCCCAAATGA GATTGGAAAT TTTGGCCGTG GAGATGTGCT GCCACCAGTT
    CCAGGCCAAG GGGATAAAAC AGCAACGATG CTCTCAGATG GAGCCATTTA TAGTAGCATT
    GACTTCACTA CCAAAACCAG TTACAACAGT TCCAGCCAAA TAACACAGGC TACCCCATAT
    GCCACGACAC AGATCTTGCA TTCCAACAGC ATACATGAAT TGGCTGTCGA TCTGCCTGAT
    CCACAATGGA AAAGCTCAAT TCAGCAAAAA ACAGATCTGA TGGGATTTGG TTATTCTCTA
    CCTGATCAGA ACAAAGGTAA CAATGGTGGG AAAGGTGGAA AAAAGAAGAA AAATAAAAAC
    TCTTCTAAAC CACAGAAAAA CAATGGATCC ACTTGGGCCA ATGTCCCTCT ACCTCCCCCC
    CCAGTCCAGC CCCTTCCTGG CACGGAGCTG GAACACTATG CAGTGGAACA ACAAGAAAAT
    GGCTATGACA GTGATAGCTG GTGCCCACCA TTGCCAGTAC AAACTTACTT ACACCAAGGT
    CTGGAAGATG AACTGGAAGA AGATGATGAT AGGGTCCCAA CACCTCCTGT TCGAGGCGTG
    GCTTCTTCTC CTGCTATCTC CTTTGGACAG CAGTCCACTG CAACTCTTAC TCCATCCCCA
    CGGGAAGAGA TGCAACCCAT GCTGCAGGCT CACCTGGATG AGTTGACAAG AGCCTATCAG
    TTTGATATAG CAAAACAAAC ATGGCACATT CAAAGCAATA ATCAACCTCC ACAGCCTCCA
    GTTCCACCGT TAGGTTATGT GTCTGGAGCC TTGATTTCTG ATTTGGAAAC GGATGTTGCA
    GATGATGATG CCGACGACGA AGAGGAAGCT TTAGAAATCC CCAGGCCCCT GAGAGCACTG
    GACCAGACTC CTGGATCCAG CATGGACAAT CTAGACAGCT CTGTGACAGG AAAAGCCTTT
    ACCTCCTCTC AAAGACCTCG ACCTACCAGC CCATTTTCTA CTGACAGTAA CACCAGTGCA
    GCCCTGAGTC AAAGTCAGAG GCCTCGGCCC ACTAAAAAAC ACAAGGGAGG GCGGATGGAC
    CAACAACCAG CATTGCCTCA TCGAAGGGAA GGAATGACAG ATGAGGAGGC CTTGGTGCCC
    TATAGCAAGC CCAGTTTCCC ATCTCCAGGT GGCCACAGCT CATCAGGAAC AGCTTCTTCT
    AAGGGATCCA CTGGACCTAG GAAAACCGAG GTGTTGAGAG CAGGCCACCA GCGCAATGCC
    AGCGACCTTC TTGACATAGG ATATATGGGC TCCAACAGTC AAGGACAGTT TACAGGTGAA
    TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016862492.1
    CDS400..4605
    Translation

    Target ORF information:

    RefSeq Version XM_017007003.1
    Organism Homo sapiens(human)
    Definition Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017007003.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
    CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT
    TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT
    CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC
    GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA
    AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC
    CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG
    TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA
    CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT
    CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCCCCC ACAGTTTGTG
    GTTCGGCCAA GAGATCAGAT TGTTGCTCAA GGTCGAACAG TGACATTTCC CTGTGAAACT
    AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAGAAAGAAG GCAGCCAGAA CCTACTTTTC
    CCAAACCAAC CCCAGCAGCC CAACAGTAGA TGCTCAGTGT CACCAACTGG AGACCTCACA
    ATCACCAACA TTCAACGTTC CGACGCGGGT TACTACATCT GCCAGGCTTT AACTGTGGCA
    GGAAGCATTT TAGCAAAAGC TCAACTGGAG GTTACTGATG TTTTGACAGA TAGACCTCCA
    CCTATAATTC TACAAGGCCC AGCCAACCAA ACGCTGGCAG TGGATGGTAC AGCGTTACTG
    AAATGTAAAG CCACTGGTGA TCCTCTTCCT GTAATTAGCT GGTTAAAGGA GGGATTTACT
    TTTCCGGGTA GAGATCCAAG AGCAACAATT CAAGAGCAAG GCACACTGCA GATTAAGAAT
    TTACGGATTT CTGATACTGG CACTTATACT TGTGTGGCTA CAAGTTCAAG TGGAGAGACT
    TCCTGGAGTG CAGTGCTGGA TGTGACAGAG TCTGGAGCAA CAATCAGTAA AAACTATGAT
    TTAAGTGACC TGCCAGGGCC ACCATCCAAA CCGCAGGTCA CTGATGTTAC TAAGAACAGT
    GTCACCTTGT CCTGGCAGCC AGGTACCCCT GGAACCCTTC CAGCAAGTGC ATATATCATT
    GAGGCTTTCA GCCAATCAGT GAGCAACAGC TGGCAGACCG TGGCAAACCA TGTAAAGACC
    ACCCTCTATA CTGTAAGAGG ACTGCGGCCC AATACAATCT ACTTATTCAT GGTCAGAGCG
    ATCAACCCCC AAGGTCTCAG TGACCCAAGT CCCATGTCAG ATCCTGTGCG CACACAAGAT
    ATCAGCCCAC CAGCACAAGG AGTGGACCAC AGGCAAGTGC AGAAAGAGCT AGGAGATGTC
    CTTGTCCGTC TTCATAATCC AGTTGTGCTG ACTCCCACCA CGGTTCAGGT CACATGGACG
    GTTGATCGCC AACCCCAGTT TATCCAAGGC TACCGAGTGA TGTATCGTCA GACTTCAGGT
    CTGCAGGCGA CATCTTCGTG GCAGAATTTA GATGCCAAAG TCCCGACTGA ACGAAGTGCT
    GTCTTAGTCA ACCTGAAAAA GGGGGTGACT TATGAAATTA AAGTACGGCC ATATTTTAAT
    GAGTTCCAAG GAATGGATAG TGAATCTAAA ACGGTTCGTA CTACTGAAGA AGCCCCAAGT
    GCCCCACCAC AGTCTGTCAC TGTACTGACA GTTGGAAGCT ACAATAGCAC AAGTATTAGT
    GTTTCCTGGG ATCCTCCTCC TCCAGATCAC CAGAATGGAA TTATCCAAGA ATACAAGATC
    TGGTGTCTAG GAAATGAAAC GCGATTCCAT ATCAACAAAA CTGTGGATGC AGCCATTCGG
    TCCGTAATAA TTGGTGGATT ATTCCCAGGT ATTCAATACC GGGTAGAGGT TGCAGCTAGT
    ACCAGTGCAG GGGTTGGAGT AAAGAGTGAG CCACAGCCAA TAATAATCGG GAGACGCAAT
    GAAGTTGTCA TTACTGAAAA CAATAACAGC ATAACTGAGC AAATCACTGA TGTGGTGAAG
    CAACCAGCCT TTATAGCTGG TATTGGTGGT GCCTGCTGGG TAATTCTGAT GGGTTTTAGC
    ATATGGTTGT ATTGGCGAAG AAAGAAGAGG AAGGGACTCA GTAATTATGC TGTTACGTTT
    CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGAAGCCGTC CAGGTCTTCT CAATGCTGGT
    GATCCCAGCT ATCCATGGCT TGCTGATTCT TGGCCAGCCA CGAGCTTGCC AGTAAATAAT
    AGCAACAGTG GCCCAAATGA GATTGGAAAT TTTGGCCGTG GAGATGTGCT GCCACCAGTT
    CCAGGCCAAG GGGATAAAAC AGCAACGATG CTCTCAGATG GAGCCATTTA TAGTAGCATT
    GACTTCACTA CCAAAACCAG TTACAACAGT TCCAGCCAAA TAACACAGGC TACCCCATAT
    GCCACGACAC AGATCTTGCA TTCCAACAGC ATACATGAAT TGGCTGTCGA TCTGCCTGAT
    CCACAATGGA AAAGCTCAAT TCAGCAAAAA ACAGATCTGA TGGGATTTGG TTATTCTCTA
    CCTGATCAGA ACAAAGGTAA CAATGGTGGG AAAGGTGGAA AAAAGAAGAA AAATAAAAAC
    TCTTCTAAAC CACAGAAAAA CAATGGATCC ACTTGGGCCA ATGTCCCTCT ACCTCCCCCC
    CCAGTCCAGC CCCTTCCTGG CACGGAGCTG GAACACTATG CAGTGGAACA ACAAGAAAAT
    GGCTATGACA GTGATAGCTG GTGCCCACCA TTGCCAGTAC AAACTTACTT ACACCAAGGT
    CTGGAAGATG AACTGGAAGA AGATGATGAT AGGGTCCCAA CACCTCCTGT TCGAGGCGTG
    GCTTCTTCTC CTGCTATCTC CTTTGGACAG CAGTCCACTG CAACTCTTAC TCCATCCCCA
    CGGGAAGAGA TGCAACCCAT GCTGCAGGCT CACCTGGATG AGTTGACAAG AGCCTATCAG
    TTTGATATAG CAAAACAAAC ATGGCACATT CAAAGCAATA ATCAACCTCC ACAGCCTCCA
    GTTCCACCGT TAGGTTATGT GTCTGGAGCC TTGATTTCTG ATTTGGAAAC GGATGTTGCA
    GATGATGATG CCGACGACGA AGAGGAAGCT TTAGAAATCC CCAGGCCCCT GAGAGCACTG
    GACCAGACTC CTGGATCCAG CATGGACAAT CTAGACAGCT CTGTGACAGG AAAAGCCTTT
    ACCTCCTCTC AAAGACCTCG ACCTACCAGC CCATTTTCTA CTGACAGTAA CACCAGTGCA
    GCCCTGAGTC AAAGTCAGAG GCCTCGGCCC ACTAAAAAAC ACAAGGGAGG GCGGATGGAC
    CAACAACCAG CATTGCCTCA TCGAAGGGAA GGAATGACAG ATGAGGAGGC CTTGGTGCCC
    TATAGCAAGC CCAGTTTCCC ATCTCCAGGT GGCCACAGCT CATCAGGAAC AGCTTCTTCT
    AAGGGATCCA CTGGACCTAG GAAAACCGAG GTGTTGAGAG CAGGCCACCA GCGCAATGCC
    AGCGACCTTC TTGACATAGG ATATATGGGC TCCAACAGTC AAGGACAGTT TACAGGTGAA
    TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94478
    Clone ID Related Accession (Same CDS sequence) XM_017007004.1
    Accession Version XM_017007004.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4197bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product roundabout homolog 2 isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCCAGAA GACATGAACG TGTCACTAGA AGGATGTGGA CATGGGCTCC GGGACTGTTG 
    ATGATGACTG TGGTGTTTTG GGGTCATCAG GGGAATGGAC AAGGCCAAGG ATCGCGTCTT
    CGCCAGGAGG ACTTTCCCCC GCGGATTGTG GAGCATCCTT CCGATGTCAT CGTCTCTAAG
    GGCGAGCCCA CGACTCTGAA CTGCAAGGCG GAGGGCCGGC CAACGCCCAC CATTGAGTGG
    TACAAAGATG GGGAGCGAGT GGAGACTGAC AAGGACGATC CCCGGTCCCA CAGGATGCTT
    CTGCCCAGCG GATCCTTATT CTTCTTGCGC ATCGTGCACG GGCGCAGGAG TAAACCTGAT
    GAAGGAAGCT ACGTTTGTGT TGCGAGGAAC TATCTTGGTG AAGCAGTGAG TCGAAATGCG
    TCTCTGGAAG TGGCATTGTT ACGAGATGAC TTCCGACAAA ACCCCACAGA TGTTGTAGTG
    GCAGCTGGAG AGCCTGCAAT CCTGGAGTGC CAGCCTCCCC GGGGACACCC AGAACCCACC
    ATCTACTGGA AAAAAGACAA AGTTCGAATT GATGACAAGG AAGAAAGAAT AAGTATCCGT
    GGTGGAAAAC TGATGATCTC CAATACCAGG AAAAGTGATG CAGGGATGTA TACTTGTGTT
    GGTACCAATA TGGTGGGAGA AAGGGACAGT GACCCAGCAG AGCTGACTGT CTTTGAACGA
    CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTACTGG AGGAAGAAGC TGTAGAATTT
    CGTTGTCAAG TCCAAGGAGA TCCTCAACCA ACTGTGAGGT GGAAAAAGGA TGATGCAGAC
    TTGCCAAGAG GAAGGTATGA CATCAAAGAC GATTACACAC TAAGAATTAA AAAGACCATG
    AGTACAGATG AAGGCACCTA TATGTGTATT GCTGAGAATC GGGTTGGAAA AATGGAAGCC
    TCTGCTACAC TCACCGTCCG AGCTCGCCCT GTTGCTCCCC CACAGTTTGT GGTTCGGCCA
    AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC
    CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA
    CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC
    ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT
    TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT
    CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA
    GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT
    AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT
    TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT
    GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC
    CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG
    TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC
    AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT
    ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC
    CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA
    CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT
    CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC
    CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG
    ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC
    AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA
    GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA
    CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG
    GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA
    GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA
    ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA
    GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC
    ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC
    TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG
    TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA
    GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC
    TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT
    GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA
    GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT
    ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA
    CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG
    AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG
    AACAAAGGTA ACAATGGTGG GAAAGGTGGA AAAAAGAAGA AAAATAAAAA CTCTTCTAAA
    CCACAGAAAA ACAATGGATC CACTTGGGCC AATGTCCCTC TACCTCCCCC CCCAGTCCAG
    CCCCTTCCTG GCACGGAGCT GGAACACTAT GCAGTGGAAC AACAAGAAAA TGGCTATGAC
    AGTGATAGCT GGTGCCCACC ATTGCCAGTA CAAACTTACT TACACCAAGG TCTGGAAGAT
    GAACTGGAAG AAGATGATGA TAGGGTCCCA ACACCTCCTG TTCGAGGCGT GGCTTCTTCT
    CCTGCTATCT CCTTTGGACA GCAGTCCACT GCAACTCTTA CTCCATCCCC ACGGGAAGAG
    ATGCAACCCA TGCTGCAGGC TCACCTGGAT GAGTTGACAA GAGCCTATCA GTTTGATATA
    GCAAAACAAA CATGGCACAT TCAAAGCAAT AATCAACCTC CACAGCCTCC AGTTCCACCG
    TTAGGTTATG TGTCTGGAGC CTTGATTTCT GATTTGGAAA CGGATGTTGC AGATGATGAT
    GCCGACGACG AAGAGGAAGC TTTAGAAATC CCCAGGCCCC TGAGAGCACT GGACCAGACT
    CCTGGATCCA GCATGGACAA TCTAGACAGC TCTGTGACAG GAAAAGCCTT TACCTCCTCT
    CAAAGACCTC GACCTACCAG CCCATTTTCT ACTGACAGTA ACACCAGTGC AGCCCTGAGT
    CAAAGTCAGA GGCCTCGGCC CACTAAAAAA CACAAGGGAG GGCGGATGGA CCAACAACCA
    GCATTGCCTC ATCGAAGGGA AGGAATGACA GATGAGGAGG CCTTGGTGCC CTATAGCAAG
    CCCAGTTTCC CATCTCCAGG TGGCCACAGC TCATCAGGAA CAGCTTCTTC TAAGGGATCC
    ACTGGACCTA GGAAAACCGA GGTGTTGAGA GCAGGCCACC AGCGCAATGC CAGCGACCTT
    CTTGACATAG GATATATGGG CTCCAACAGT CAAGGACAGT TTACAGGTGA ATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016862493.1
    CDS300..4496
    Translation

    Target ORF information:

    RefSeq Version XM_017007004.1
    Organism Homo sapiens(human)
    Definition Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017007004.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCCAGAA GACATGAACG TGTCACTAGA AGGATGTGGA CATGGGCTCC GGGACTGTTG 
    ATGATGACTG TGGTGTTTTG GGGTCATCAG GGGAATGGAC AAGGCCAAGG ATCGCGTCTT
    CGCCAGGAGG ACTTTCCCCC GCGGATTGTG GAGCATCCTT CCGATGTCAT CGTCTCTAAG
    GGCGAGCCCA CGACTCTGAA CTGCAAGGCG GAGGGCCGGC CAACGCCCAC CATTGAGTGG
    TACAAAGATG GGGAGCGAGT GGAGACTGAC AAGGACGATC CCCGGTCCCA CAGGATGCTT
    CTGCCCAGCG GATCCTTATT CTTCTTGCGC ATCGTGCACG GGCGCAGGAG TAAACCTGAT
    GAAGGAAGCT ACGTTTGTGT TGCGAGGAAC TATCTTGGTG AAGCAGTGAG TCGAAATGCG
    TCTCTGGAAG TGGCATTGTT ACGAGATGAC TTCCGACAAA ACCCCACAGA TGTTGTAGTG
    GCAGCTGGAG AGCCTGCAAT CCTGGAGTGC CAGCCTCCCC GGGGACACCC AGAACCCACC
    ATCTACTGGA AAAAAGACAA AGTTCGAATT GATGACAAGG AAGAAAGAAT AAGTATCCGT
    GGTGGAAAAC TGATGATCTC CAATACCAGG AAAAGTGATG CAGGGATGTA TACTTGTGTT
    GGTACCAATA TGGTGGGAGA AAGGGACAGT GACCCAGCAG AGCTGACTGT CTTTGAACGA
    CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTACTGG AGGAAGAAGC TGTAGAATTT
    CGTTGTCAAG TCCAAGGAGA TCCTCAACCA ACTGTGAGGT GGAAAAAGGA TGATGCAGAC
    TTGCCAAGAG GAAGGTATGA CATCAAAGAC GATTACACAC TAAGAATTAA AAAGACCATG
    AGTACAGATG AAGGCACCTA TATGTGTATT GCTGAGAATC GGGTTGGAAA AATGGAAGCC
    TCTGCTACAC TCACCGTCCG AGCTCGCCCT GTTGCTCCCC CACAGTTTGT GGTTCGGCCA
    AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC
    CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA
    CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC
    ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT
    TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT
    CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA
    GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT
    AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT
    TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT
    GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC
    CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG
    TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC
    AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT
    ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC
    CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA
    CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT
    CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC
    CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG
    ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC
    AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA
    GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA
    CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG
    GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA
    GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA
    ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA
    GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC
    ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC
    TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG
    TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA
    GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC
    TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT
    GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA
    GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT
    ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA
    CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG
    AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG
    AACAAAGGTA ACAATGGTGG GAAAGGTGGA AAAAAGAAGA AAAATAAAAA CTCTTCTAAA
    CCACAGAAAA ACAATGGATC CACTTGGGCC AATGTCCCTC TACCTCCCCC CCCAGTCCAG
    CCCCTTCCTG GCACGGAGCT GGAACACTAT GCAGTGGAAC AACAAGAAAA TGGCTATGAC
    AGTGATAGCT GGTGCCCACC ATTGCCAGTA CAAACTTACT TACACCAAGG TCTGGAAGAT
    GAACTGGAAG AAGATGATGA TAGGGTCCCA ACACCTCCTG TTCGAGGCGT GGCTTCTTCT
    CCTGCTATCT CCTTTGGACA GCAGTCCACT GCAACTCTTA CTCCATCCCC ACGGGAAGAG
    ATGCAACCCA TGCTGCAGGC TCACCTGGAT GAGTTGACAA GAGCCTATCA GTTTGATATA
    GCAAAACAAA CATGGCACAT TCAAAGCAAT AATCAACCTC CACAGCCTCC AGTTCCACCG
    TTAGGTTATG TGTCTGGAGC CTTGATTTCT GATTTGGAAA CGGATGTTGC AGATGATGAT
    GCCGACGACG AAGAGGAAGC TTTAGAAATC CCCAGGCCCC TGAGAGCACT GGACCAGACT
    CCTGGATCCA GCATGGACAA TCTAGACAGC TCTGTGACAG GAAAAGCCTT TACCTCCTCT
    CAAAGACCTC GACCTACCAG CCCATTTTCT ACTGACAGTA ACACCAGTGC AGCCCTGAGT
    CAAAGTCAGA GGCCTCGGCC CACTAAAAAA CACAAGGGAG GGCGGATGGA CCAACAACCA
    GCATTGCCTC ATCGAAGGGA AGGAATGACA GATGAGGAGG CCTTGGTGCC CTATAGCAAG
    CCCAGTTTCC CATCTCCAGG TGGCCACAGC TCATCAGGAA CAGCTTCTTC TAAGGGATCC
    ACTGGACCTA GGAAAACCGA GGTGTTGAGA GCAGGCCACC AGCGCAATGC CAGCGACCTT
    CTTGACATAG GATATATGGG CTCCAACAGT CAAGGACAGT TTACAGGTGA ATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94479
    Clone ID Related Accession (Same CDS sequence) XM_017007005.1
    Accession Version XM_017007005.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4191bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product roundabout homolog 2 isoform X25
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCCAGAA GACATGAACG TGTCACTAGA AGGATGTGGA CATGGGCTCC GGGACTGTTG 
    ATGATGACTG TGGTGTTTTG GGGTCATCAG GGGAATGGAC AAGGCCAAGG ATCGCGTCTT
    CGCCAGGAGG ACTTTCCCCC GCGGATTGTG GAGCATCCTT CCGATGTCAT CGTCTCTAAG
    GGCGAGCCCA CGACTCTGAA CTGCAAGGCG GAGGGCCGGC CAACGCCCAC CATTGAGTGG
    TACAAAGATG GGGAGCGAGT GGAGACTGAC AAGGACGATC CCCGGTCCCA CAGGATGCTT
    CTGCCCAGCG GATCCTTATT CTTCTTGCGC ATCGTGCACG GGCGCAGGAG TAAACCTGAT
    GAAGGAAGCT ACGTTTGTGT TGCGAGGAAC TATCTTGGTG AAGCAGTGAG TCGAAATGCG
    TCTCTGGAAG TGGCATTGTT ACGAGATGAC TTCCGACAAA ACCCCACAGA TGTTGTAGTG
    GCAGCTGGAG AGCCTGCAAT CCTGGAGTGC CAGCCTCCCC GGGGACACCC AGAACCCACC
    ATCTACTGGA AAAAAGACAA AGTTCGAATT GATGACAAGG AAGAAAGAAT AAGTATCCGT
    GGTGGAAAAC TGATGATCTC CAATACCAGG AAAAGTGATG CAGGGATGTA TACTTGTGTT
    GGTACCAATA TGGTGGGAGA AAGGGACAGT GACCCAGCAG AGCTGACTGT CTTTGAACGA
    CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTACTGG AGGAAGAAGC TGTAGAATTT
    CGTTGTCAAG TCCAAGGAGA TCCTCAACCA ACTGTGAGGT GGAAAAAGGA TGATGCAGAC
    TTGCCAAGAG GAAGGTATGA CATCAAAGAC GATTACACAC TAAGAATTAA AAAGACCATG
    AGTACAGATG AAGGCACCTA TATGTGTATT GCTGAGAATC GGGTTGGAAA AATGGAAGCC
    TCTGCTACAC TCACCGTCCG AGCTCGCCCT GTTGCTCCCC CACAGTTTGT GGTTCGGCCA
    AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC
    CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA
    CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC
    ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT
    TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT
    CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA
    GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT
    AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT
    TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT
    GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC
    CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG
    TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC
    AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT
    ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC
    CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA
    CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT
    CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC
    CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG
    ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC
    AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA
    GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA
    CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG
    GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA
    GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA
    ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA
    GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC
    ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC
    TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG
    TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA
    GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC
    TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT
    GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA
    GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT
    ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA
    CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG
    AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG
    AACAAAGGTA ACAATGGTGG GAAAGGTGGA AAAAAGAAGA AAAATAAAAA CTCTTCTAAA
    CCACAGAAAA ACAATGGATC CACTTGGGCC AATGTCCCTC TACCTCCCCC CCCAGTCCAG
    CCCCTTCCTG GCACGGAGCT GGAACACTAT GCAGTGGAAC AACAAGAAAA TGGCTATGAC
    AGTGATAGCT GGTGCCCACC ATTGCCAGTA CAAACTTACT TACACCAAGG TCTGGAAGAT
    GAACTGGAAG AAGATGATGA TAGGGTCCCA ACACCTCCTG TTCGAGGCGT GGCTTCTTCT
    CCTGCTATCT CCTTTGGACA GCAGTCCACT GCAACTCTTA CTCCATCCCC ACGGGAAGAG
    ATGCAACCCA TGCTGCAGGC TCACCTGGAT GAGTTGACAA GAGCCTATCA GTTTGATATA
    GCAAAACAAA CATGGCACAT TCAAAGCAAT AATCAACCTC CACAGCCTCC AGTTCCACCG
    TTAGGTTATG TGTCTGGAGC CTTGATTTCT GATTTGGAAA CGGATGTTGC AGATGATGAT
    GCCGACGACG AAGAGGAAGC TTTAGAAATC CCCAGGCCCC TGAGAGCACT GGACCAGACT
    CCTGGATCCA GCATGGACAA TCTAGACAGC TCTGTGACAG GAAAAGCCTT TACCTCCTCT
    CAAAGACCTC GACCTACCAG CCCATTTTCT ACTGACAGTA ACACCAGTGC AGCCCTGAGT
    CAAAGTCAGA GGCCTCGGCC CACTAAAAAA CACAAGGGAG GGCGGATGGA CCAACAACCA
    GCATTGCCTC ATCGAAGGGA AGGAATGACA GATGAGGAGG CCTTGGTGCC CTATAGCAAG
    CCCAGTTTCC CATCTCCAGG TGGCCACAGC TCATCAGGAA CAGCTTCTTC TAAGGGATCC
    ACTGGACCTA GGAAAACCGA GGTGTTGAGA GCAGGCCACC AGCGCAATGC CAGCGACCTT
    CTTGACATAG GATATATGGG CTCCAACAGT CAAGGACAGT TTACAGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016862494.1
    CDS300..4490
    Translation

    Target ORF information:

    RefSeq Version XM_017007005.1
    Organism Homo sapiens(human)
    Definition Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017007005.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCCAGAA GACATGAACG TGTCACTAGA AGGATGTGGA CATGGGCTCC GGGACTGTTG 
    ATGATGACTG TGGTGTTTTG GGGTCATCAG GGGAATGGAC AAGGCCAAGG ATCGCGTCTT
    CGCCAGGAGG ACTTTCCCCC GCGGATTGTG GAGCATCCTT CCGATGTCAT CGTCTCTAAG
    GGCGAGCCCA CGACTCTGAA CTGCAAGGCG GAGGGCCGGC CAACGCCCAC CATTGAGTGG
    TACAAAGATG GGGAGCGAGT GGAGACTGAC AAGGACGATC CCCGGTCCCA CAGGATGCTT
    CTGCCCAGCG GATCCTTATT CTTCTTGCGC ATCGTGCACG GGCGCAGGAG TAAACCTGAT
    GAAGGAAGCT ACGTTTGTGT TGCGAGGAAC TATCTTGGTG AAGCAGTGAG TCGAAATGCG
    TCTCTGGAAG TGGCATTGTT ACGAGATGAC TTCCGACAAA ACCCCACAGA TGTTGTAGTG
    GCAGCTGGAG AGCCTGCAAT CCTGGAGTGC CAGCCTCCCC GGGGACACCC AGAACCCACC
    ATCTACTGGA AAAAAGACAA AGTTCGAATT GATGACAAGG AAGAAAGAAT AAGTATCCGT
    GGTGGAAAAC TGATGATCTC CAATACCAGG AAAAGTGATG CAGGGATGTA TACTTGTGTT
    GGTACCAATA TGGTGGGAGA AAGGGACAGT GACCCAGCAG AGCTGACTGT CTTTGAACGA
    CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTACTGG AGGAAGAAGC TGTAGAATTT
    CGTTGTCAAG TCCAAGGAGA TCCTCAACCA ACTGTGAGGT GGAAAAAGGA TGATGCAGAC
    TTGCCAAGAG GAAGGTATGA CATCAAAGAC GATTACACAC TAAGAATTAA AAAGACCATG
    AGTACAGATG AAGGCACCTA TATGTGTATT GCTGAGAATC GGGTTGGAAA AATGGAAGCC
    TCTGCTACAC TCACCGTCCG AGCTCGCCCT GTTGCTCCCC CACAGTTTGT GGTTCGGCCA
    AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC
    CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA
    CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC
    ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT
    TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT
    CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA
    GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT
    AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT
    TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT
    GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC
    CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG
    TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC
    AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT
    ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC
    CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA
    CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT
    CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC
    CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG
    ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC
    AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA
    GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA
    CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG
    GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA
    GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA
    ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA
    GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC
    ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC
    TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG
    TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA
    GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC
    TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT
    GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA
    GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT
    ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA
    CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG
    AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG
    AACAAAGGTA ACAATGGTGG GAAAGGTGGA AAAAAGAAGA AAAATAAAAA CTCTTCTAAA
    CCACAGAAAA ACAATGGATC CACTTGGGCC AATGTCCCTC TACCTCCCCC CCCAGTCCAG
    CCCCTTCCTG GCACGGAGCT GGAACACTAT GCAGTGGAAC AACAAGAAAA TGGCTATGAC
    AGTGATAGCT GGTGCCCACC ATTGCCAGTA CAAACTTACT TACACCAAGG TCTGGAAGAT
    GAACTGGAAG AAGATGATGA TAGGGTCCCA ACACCTCCTG TTCGAGGCGT GGCTTCTTCT
    CCTGCTATCT CCTTTGGACA GCAGTCCACT GCAACTCTTA CTCCATCCCC ACGGGAAGAG
    ATGCAACCCA TGCTGCAGGC TCACCTGGAT GAGTTGACAA GAGCCTATCA GTTTGATATA
    GCAAAACAAA CATGGCACAT TCAAAGCAAT AATCAACCTC CACAGCCTCC AGTTCCACCG
    TTAGGTTATG TGTCTGGAGC CTTGATTTCT GATTTGGAAA CGGATGTTGC AGATGATGAT
    GCCGACGACG AAGAGGAAGC TTTAGAAATC CCCAGGCCCC TGAGAGCACT GGACCAGACT
    CCTGGATCCA GCATGGACAA TCTAGACAGC TCTGTGACAG GAAAAGCCTT TACCTCCTCT
    CAAAGACCTC GACCTACCAG CCCATTTTCT ACTGACAGTA ACACCAGTGC AGCCCTGAGT
    CAAAGTCAGA GGCCTCGGCC CACTAAAAAA CACAAGGGAG GGCGGATGGA CCAACAACCA
    GCATTGCCTC ATCGAAGGGA AGGAATGACA GATGAGGAGG CCTTGGTGCC CTATAGCAAG
    CCCAGTTTCC CATCTCCAGG TGGCCACAGC TCATCAGGAA CAGCTTCTTC TAAGGGATCC
    ACTGGACCTA GGAAAACCGA GGTGTTGAGA GCAGGCCACC AGCGCAATGC CAGCGACCTT
    CTTGACATAG GATATATGGG CTCCAACAGT CAAGGACAGT TTACAGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94480
    Clone ID Related Accession (Same CDS sequence) XM_017007006.1
    Accession Version XM_017007006.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4140bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product roundabout homolog 2 isoform X26
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC
    ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC
    ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC
    CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG
    AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA
    GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG
    TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA
    GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG
    GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT
    AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA
    AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCGCC CTGTTGCTCC CCCACAGTTT
    GTGGTTCGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT
    TTCCCAAACC AACCCCAGCA GCCCAACAGT AGATGCTCAG TGTCACCAAC TGGAGACCTC
    ACAATCACCA ACATTCAACG TTCCGACGCG GGTTACTACA TCTGCCAGGC TTTAACTGTG
    GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAGGTTACTG ATGTTTTGAC AGATAGACCT
    CCACCTATAA TTCTACAAGG CCCAGCCAAC CAAACGCTGG CAGTGGATGG TACAGCGTTA
    CTGAAATGTA AAGCCACTGG TGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT
    ACTTTTCCGG GTAGAGATCC AAGAGCAACA ATTCAAGAGC AAGGCACACT GCAGATTAAG
    AATTTACGGA TTTCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGAGAG
    ACTTCCTGGA GTGCAGTGCT GGATGTGACA GAGTCTGGAG CAACAATCAG TAAAAACTAT
    GATTTAAGTG ACCTGCCAGG GCCACCATCC AAACCGCAGG TCACTGATGT TACTAAGAAC
    AGTGTCACCT TGTCCTGGCA GCCAGGTACC CCTGGAACCC TTCCAGCAAG TGCATATATC
    ATTGAGGCTT TCAGCCAATC AGTGAGCAAC AGCTGGCAGA CCGTGGCAAA CCATGTAAAG
    ACCACCCTCT ATACTGTAAG AGGACTGCGG CCCAATACAA TCTACTTATT CATGGTCAGA
    GCGATCAACC CCCAAGGTCT CAGTGACCCA AGTCCCATGT CAGATCCTGT GCGCACACAA
    GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TGCAGAAAGA GCTAGGAGAT
    GTCCTTGTCC GTCTTCATAA TCCAGTTGTG CTGACTCCCA CCACGGTTCA GGTCACATGG
    ACGGTTGATC GCCAACCCCA GTTTATCCAA GGCTACCGAG TGATGTATCG TCAGACTTCA
    GGTCTGCAGG CGACATCTTC GTGGCAGAAT TTAGATGCCA AAGTCCCGAC TGAACGAAGT
    GCTGTCTTAG TCAACCTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTACG GCCATATTTT
    AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACGGTTC GTACTACTGA AGAAGCCCCA
    AGTGCCCCAC CACAGTCTGT CACTGTACTG ACAGTTGGAA GCTACAATAG CACAAGTATT
    AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CACCAGAATG GAATTATCCA AGAATACAAG
    ATCTGGTGTC TAGGAAATGA AACGCGATTC CATATCAACA AAACTGTGGA TGCAGCCATT
    CGGTCCGTAA TAATTGGTGG ATTATTCCCA GGTATTCAAT ACCGGGTAGA GGTTGCAGCT
    AGTACCAGTG CAGGGGTTGG AGTAAAGAGT GAGCCACAGC CAATAATAAT CGGGAGACGC
    AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTGGTG
    AAGCAACCAG CCTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT
    AGCATATGGT TGTATTGGCG AAGAAAGAAG AGGAAGGGAC TCAGTAATTA TGCTGTTACG
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GTCCAGGTCT TCTCAATGCT
    GGTGATCCCA GCTATCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGCTT GCCAGTAAAT
    AATAGCAACA GTGGCCCAAA TGAGATTGGA AATTTTGGCC GTGGAGATGT GCTGCCACCA
    GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGTAGC
    ATTGACTTCA CTACCAAAAC CAGTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA
    TATGCCACGA CACAGATCTT GCATTCCAAC AGCATACATG AATTGGCTGT CGATCTGCCT
    GATCCACAAT GGAAAAGCTC AATTCAGCAA AAAACAGATC TGATGGGATT TGGTTATTCT
    CTACCTGATC AGAACAAAGG TGGGAAAGGT GGAAAAAAGA AGAAAAATAA AAACTCTTCT
    AAACCACAGA AAAACAATGG ATCCACTTGG GCCAATGTCC CTCTACCTCC CCCCCCAGTC
    CAGCCCCTTC CTGGCACGGA GCTGGAACAC TATGCAGTGG AACAACAAGA AAATGGCTAT
    GACAGTGATA GCTGGTGCCC ACCATTGCCA GTACAAACTT ACTTACACCA AGGTCTGGAA
    GATGAACTGG AAGAAGATGA TGATAGGGTC CCAACACCTC CTGTTCGAGG CGTGGCTTCT
    TCTCCTGCTA TCTCCTTTGG ACAGCAGTCC ACTGCAACTC TTACTCCATC CCCACGGGAA
    GAGATGCAAC CCATGCTGCA GGCTCACCTG GATGAGTTGA CAAGAGCCTA TCAGTTTGAT
    ATAGCAAAAC AAACATGGCA CATTCAAAGC AATAATCAAC CTCCACAGCC TCCAGTTCCA
    CCGTTAGGTT ATGTGTCTGG AGCCTTGATT TCTGATTTGG AAACGGATGT TGCAGATGAT
    GATGCCGACG ACGAAGAGGA AGCTTTAGAA ATCCCCAGGC CCCTGAGAGC ACTGGACCAG
    ACTCCTGGAT CCAGCATGGA CAATCTAGAC AGCTCTGTGA CAGGAAAAGC CTTTACCTCC
    TCTCAAAGAC CTCGACCTAC CAGCCCATTT TCTACTGACA GTAACACCAG TGCAGCCCTG
    AGTCAAAGTC AGAGGCCTCG GCCCACTAAA AAACACAAGG GAGGGCGGAT GGACCAACAA
    CCAGCATTGC CTCATCGAAG GGAAGGAATG ACAGATGAGG AGGCCTTGGT GCCCTATAGC
    AAGCCCAGTT TCCCATCTCC AGGTGGCCAC AGCTCATCAG GAACAGCTTC TTCTAAGGGA
    TCCACTGGAC CTAGGAAAAC CGAGGTGTTG AGAGCAGGCC ACCAGCGCAA TGCCAGCGAC
    CTTCTTGACA TAGGATATAT GGGCTCCAAC AGTCAAGGAC AGTTTACAGG TGAATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016862495.1
    CDS692..4831
    Translation

    Target ORF information:

    RefSeq Version XM_017007006.1
    Organism Homo sapiens(human)
    Definition Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X26, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017007006.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC
    ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC
    ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC
    CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG
    AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA
    GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG
    TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA
    GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG
    GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT
    AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA
    AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCGCC CTGTTGCTCC CCCACAGTTT
    GTGGTTCGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT
    TTCCCAAACC AACCCCAGCA GCCCAACAGT AGATGCTCAG TGTCACCAAC TGGAGACCTC
    ACAATCACCA ACATTCAACG TTCCGACGCG GGTTACTACA TCTGCCAGGC TTTAACTGTG
    GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAGGTTACTG ATGTTTTGAC AGATAGACCT
    CCACCTATAA TTCTACAAGG CCCAGCCAAC CAAACGCTGG CAGTGGATGG TACAGCGTTA
    CTGAAATGTA AAGCCACTGG TGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT
    ACTTTTCCGG GTAGAGATCC AAGAGCAACA ATTCAAGAGC AAGGCACACT GCAGATTAAG
    AATTTACGGA TTTCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGAGAG
    ACTTCCTGGA GTGCAGTGCT GGATGTGACA GAGTCTGGAG CAACAATCAG TAAAAACTAT
    GATTTAAGTG ACCTGCCAGG GCCACCATCC AAACCGCAGG TCACTGATGT TACTAAGAAC
    AGTGTCACCT TGTCCTGGCA GCCAGGTACC CCTGGAACCC TTCCAGCAAG TGCATATATC
    ATTGAGGCTT TCAGCCAATC AGTGAGCAAC AGCTGGCAGA CCGTGGCAAA CCATGTAAAG
    ACCACCCTCT ATACTGTAAG AGGACTGCGG CCCAATACAA TCTACTTATT CATGGTCAGA
    GCGATCAACC CCCAAGGTCT CAGTGACCCA AGTCCCATGT CAGATCCTGT GCGCACACAA
    GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TGCAGAAAGA GCTAGGAGAT
    GTCCTTGTCC GTCTTCATAA TCCAGTTGTG CTGACTCCCA CCACGGTTCA GGTCACATGG
    ACGGTTGATC GCCAACCCCA GTTTATCCAA GGCTACCGAG TGATGTATCG TCAGACTTCA
    GGTCTGCAGG CGACATCTTC GTGGCAGAAT TTAGATGCCA AAGTCCCGAC TGAACGAAGT
    GCTGTCTTAG TCAACCTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTACG GCCATATTTT
    AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACGGTTC GTACTACTGA AGAAGCCCCA
    AGTGCCCCAC CACAGTCTGT CACTGTACTG ACAGTTGGAA GCTACAATAG CACAAGTATT
    AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CACCAGAATG GAATTATCCA AGAATACAAG
    ATCTGGTGTC TAGGAAATGA AACGCGATTC CATATCAACA AAACTGTGGA TGCAGCCATT
    CGGTCCGTAA TAATTGGTGG ATTATTCCCA GGTATTCAAT ACCGGGTAGA GGTTGCAGCT
    AGTACCAGTG CAGGGGTTGG AGTAAAGAGT GAGCCACAGC CAATAATAAT CGGGAGACGC
    AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTGGTG
    AAGCAACCAG CCTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT
    AGCATATGGT TGTATTGGCG AAGAAAGAAG AGGAAGGGAC TCAGTAATTA TGCTGTTACG
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GTCCAGGTCT TCTCAATGCT
    GGTGATCCCA GCTATCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGCTT GCCAGTAAAT
    AATAGCAACA GTGGCCCAAA TGAGATTGGA AATTTTGGCC GTGGAGATGT GCTGCCACCA
    GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGTAGC
    ATTGACTTCA CTACCAAAAC CAGTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA
    TATGCCACGA CACAGATCTT GCATTCCAAC AGCATACATG AATTGGCTGT CGATCTGCCT
    GATCCACAAT GGAAAAGCTC AATTCAGCAA AAAACAGATC TGATGGGATT TGGTTATTCT
    CTACCTGATC AGAACAAAGG TGGGAAAGGT GGAAAAAAGA AGAAAAATAA AAACTCTTCT
    AAACCACAGA AAAACAATGG ATCCACTTGG GCCAATGTCC CTCTACCTCC CCCCCCAGTC
    CAGCCCCTTC CTGGCACGGA GCTGGAACAC TATGCAGTGG AACAACAAGA AAATGGCTAT
    GACAGTGATA GCTGGTGCCC ACCATTGCCA GTACAAACTT ACTTACACCA AGGTCTGGAA
    GATGAACTGG AAGAAGATGA TGATAGGGTC CCAACACCTC CTGTTCGAGG CGTGGCTTCT
    TCTCCTGCTA TCTCCTTTGG ACAGCAGTCC ACTGCAACTC TTACTCCATC CCCACGGGAA
    GAGATGCAAC CCATGCTGCA GGCTCACCTG GATGAGTTGA CAAGAGCCTA TCAGTTTGAT
    ATAGCAAAAC AAACATGGCA CATTCAAAGC AATAATCAAC CTCCACAGCC TCCAGTTCCA
    CCGTTAGGTT ATGTGTCTGG AGCCTTGATT TCTGATTTGG AAACGGATGT TGCAGATGAT
    GATGCCGACG ACGAAGAGGA AGCTTTAGAA ATCCCCAGGC CCCTGAGAGC ACTGGACCAG
    ACTCCTGGAT CCAGCATGGA CAATCTAGAC AGCTCTGTGA CAGGAAAAGC CTTTACCTCC
    TCTCAAAGAC CTCGACCTAC CAGCCCATTT TCTACTGACA GTAACACCAG TGCAGCCCTG
    AGTCAAAGTC AGAGGCCTCG GCCCACTAAA AAACACAAGG GAGGGCGGAT GGACCAACAA
    CCAGCATTGC CTCATCGAAG GGAAGGAATG ACAGATGAGG AGGCCTTGGT GCCCTATAGC
    AAGCCCAGTT TCCCATCTCC AGGTGGCCAC AGCTCATCAG GAACAGCTTC TTCTAAGGGA
    TCCACTGGAC CTAGGAAAAC CGAGGTGTTG AGAGCAGGCC ACCAGCGCAA TGCCAGCGAC
    CTTCTTGACA TAGGATATAT GGGCTCCAAC AGTCAAGGAC AGTTTACAGG TGAATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94460
    Clone ID Related Accession (Same CDS sequence) XM_017006986.1
    Accession Version XM_017006986.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4731bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product roundabout homolog 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC
    ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC
    ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC
    CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG
    AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA
    GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG
    TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA
    GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG
    GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT
    AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA
    AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCGCC CTGTTGCTCC CCCACAGTTT
    GTGGTTCGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT
    TTCCCAAACC AACCCCAGCA GCCCAACAGT AGATGCTCAG TGTCACCAAC TGGAGACCTC
    ACAATCACCA ACATTCAACG TTCCGACGCG GGTTACTACA TCTGCCAGGC TTTAACTGTG
    GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAGGTTACTG ATGTTTTGAC AGATAGACCT
    CCACCTATAA TTCTACAAGG CCCAGCCAAC CAAACGCTGG CAGTGGATGG TACAGCGTTA
    CTGAAATGTA AAGCCACTGG TGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT
    ACTTTTCCGG GTAGAGATCC AAGAGCAACA ATTCAAGAGC AAGGCACACT GCAGATTAAG
    AATTTACGGA TTTCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGAGAG
    ACTTCCTGGA GTGCAGTGCT GGATGTGACA GAGTCTGGAG CAACAATCAG TAAAAACTAT
    GATTTAAGTG ACCTGCCAGG GCCACCATCC AAACCGCAGG TCACTGATGT TACTAAGAAC
    AGTGTCACCT TGTCCTGGCA GCCAGGTACC CCTGGAACCC TTCCAGCAAG TGCATATATC
    ATTGAGGCTT TCAGCCAATC AGTGAGCAAC AGCTGGCAGA CCGTGGCAAA CCATGTAAAG
    ACCACCCTCT ATACTGTAAG AGGACTGCGG CCCAATACAA TCTACTTATT CATGGTCAGA
    GCGATCAACC CCCAAGGTCT CAGTGACCCA AGTCCCATGT CAGATCCTGT GCGCACACAA
    GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TGCAGAAAGA GCTAGGAGAT
    GTCCTTGTCC GTCTTCATAA TCCAGTTGTG CTGACTCCCA CCACGGTTCA GGTCACATGG
    ACGGTTGATC GCCAACCCCA GTTTATCCAA GGCTACCGAG TGATGTATCG TCAGACTTCA
    GGTCTGCAGG CGACATCTTC GTGGCAGAAT TTAGATGCCA AAGTCCCGAC TGAACGAAGT
    GCTGTCTTAG TCAACCTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTACG GCCATATTTT
    AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACGGTTC GTACTACTGA AGAAGCCCCA
    AGTGCCCCAC CACAGTCTGT CACTGTACTG ACAGTTGGAA GCTACAATAG CACAAGTATT
    AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CACCAGAATG GAATTATCCA AGAATACAAG
    ATCTGGTGTC TAGGAAATGA AACGCGATTC CATATCAACA AAACTGTGGA TGCAGCCATT
    CGGTCCGTAA TAATTGGTGG ATTATTCCCA GGTATTCAAT ACCGGGTAGA GGTTGCAGCT
    AGTACCAGTG CAGGGGTTGG AGTAAAGAGT GAGCCACAGC CAATAATAAT CGGGAGACGC
    AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTGGTG
    AAGCAACCAG CCTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT
    AGCATATGGT TGTATTGGCG AAGAAAGAAG AGGAAGGGAC TCAGTAATTA TGCTGTTACG
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GTCCAGGTCT TCTCAATGCT
    GGTGATCCCA GCTATCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGCTT GCCAGTAAAT
    AATAGCAACA GTGGCCCAAA TGAGATTGGA AATTTTGGCC GTGGAGATGT GCTGCCACCA
    GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGTAGC
    ATTGACTTCA CTACCAAAAC CAGTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA
    TATGCCACGA CACAGATCTT GCATTCCAAC AGCATACATG AATTGGCTGT CGATCTGCCT
    GATCCACAAT GGAAAAGCTC AATTCAGCAA AAAACAGATC TGATGGGATT TGGTTATTCT
    CTACCTGATC AGAACAAAGG TAACAATGCC TTACTTTACA TTCCTGACTA CCGATTGGCT
    GAGGGATTGT CTAATAGAAT GCCACACAAC CAGTCTCAGG ATTTCAGCAC CACCAGCTCT
    CACAACAGCT CAGAAAGGAG TGGCAGTCTC TCAGGTGGGA AAGGTGGAAA AAAGAAGAAA
    AATAAAAACT CTTCTAAACC ACAGAAAAAC AATGGATCCA CTTGGGCCAA TGTCCCTCTA
    CCTCCCCCCC CAGTCCAGCC CCTTCCTGGC ACGGAGCTGG AACACTATGC AGTGGAACAA
    CAAGAAAATG GCTATGACAG TGATAGCTGG TGCCCACCAT TGCCAGTACA AACTTACTTA
    CACCAAGGTC TGGAAGATGA ACTGGAAGAA GATGATGATA GGGTCCCAAC ACCTCCTGTT
    CGAGGCGTGG CTTCTTCTCC TGCTATCTCC TTTGGACAGC AGTCCACTGC AACTCTTACT
    CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAGGCTC ACCTGGATGA GTTGACAAGA
    GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAA TCAACCTCCA
    CAGCCTCCAG TTCCACCGTT AGGTTATGTG TCTGGAGCCT TGATTTCTGA TTTGGAAACG
    GATGTTGCAG ATGATGATGC CGACGACGAA GAGGAAGCTT TAGAAATCCC CAGGCCCCTG
    AGAGCACTGG ACCAGACTCC TGGATCCAGC ATGGACAATC TAGACAGCTC TGTGACAGGT
    TCAATGGTAA ATGGATGGGG CTCTGCATCT GATGAGGATC GTAACTTTTC TAGTCATAGA
    TCTAGTGTAG GTAGCTCCTC AGATGGCTCT ATCTTTGCCA GCGGCAGTTT TGCACAAGCA
    CTGGTGGCAG CAGCAGATAA AGCTGGTTTT AGGCTGGATG GAACCAGCCT TACAAGAACA
    GGAAAAGCCT TTACCTCCTC TCAAAGACCT CGACCTACCA GCCCATTTTC TACTGACAGT
    AACACCAGTG CAGCCCTGAG TCAAAGTCAG AGGCCTCGGC CCACTAAAAA ACACAAGGGA
    GGGCGGATGG ACCAACAACC AGCATTGCCT CATCGAAGGG AAGGAATGAC AGATGATCTT
    CCACCACCAC CAGATCCCCC GCCAGGTCAG GGTTTAAGGC AGCAAATAGG CCCGAGCCAG
    CAGGCTGGTA ACGTGGAAAA CTCAGCAGAG AGAAAAGGAA GCTCTCTAGA GAGACAACAT
    GCATCCAGCT TAGAAGACAC AAAGAGCTCA TTGGATTGTC CAGCTAGAAC CTCCCTAGAG
    TGGCAGCGAC AAACCCAGGA ATGGATAAGC TCCACAGAAC GACAAGAAGA TATACGGAAA
    GCCCCACACA AACAAGGTGT CGGATCAGAG GAGGCCTTGG TGCCCTATAG CAAGCCCAGT
    TTCCCATCTC CAGGTGGCCA CAGCTCATCA GGAACAGCTT CTTCTAAGGG ATCCACTGGA
    CCTAGGAAAA CCGAGGTGTT GAGAGCAGGC CACCAGCGCA ATGCCAGCGA CCTTCTTGAC
    ATAGGATATA TGGGCTCCAA CAGTCAAGGA CAGTTTACAG GTGAATTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016862475.1
    CDS692..5422
    Translation

    Target ORF information:

    RefSeq Version XM_017006986.1
    Organism Homo sapiens(human)
    Definition Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017006986.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC
    ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC
    ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC
    CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG
    AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA
    GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG
    TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA
    GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG
    GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT
    AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA
    AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCGCC CTGTTGCTCC CCCACAGTTT
    GTGGTTCGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT
    TTCCCAAACC AACCCCAGCA GCCCAACAGT AGATGCTCAG TGTCACCAAC TGGAGACCTC
    ACAATCACCA ACATTCAACG TTCCGACGCG GGTTACTACA TCTGCCAGGC TTTAACTGTG
    GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAGGTTACTG ATGTTTTGAC AGATAGACCT
    CCACCTATAA TTCTACAAGG CCCAGCCAAC CAAACGCTGG CAGTGGATGG TACAGCGTTA
    CTGAAATGTA AAGCCACTGG TGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT
    ACTTTTCCGG GTAGAGATCC AAGAGCAACA ATTCAAGAGC AAGGCACACT GCAGATTAAG
    AATTTACGGA TTTCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGAGAG
    ACTTCCTGGA GTGCAGTGCT GGATGTGACA GAGTCTGGAG CAACAATCAG TAAAAACTAT
    GATTTAAGTG ACCTGCCAGG GCCACCATCC AAACCGCAGG TCACTGATGT TACTAAGAAC
    AGTGTCACCT TGTCCTGGCA GCCAGGTACC CCTGGAACCC TTCCAGCAAG TGCATATATC
    ATTGAGGCTT TCAGCCAATC AGTGAGCAAC AGCTGGCAGA CCGTGGCAAA CCATGTAAAG
    ACCACCCTCT ATACTGTAAG AGGACTGCGG CCCAATACAA TCTACTTATT CATGGTCAGA
    GCGATCAACC CCCAAGGTCT CAGTGACCCA AGTCCCATGT CAGATCCTGT GCGCACACAA
    GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TGCAGAAAGA GCTAGGAGAT
    GTCCTTGTCC GTCTTCATAA TCCAGTTGTG CTGACTCCCA CCACGGTTCA GGTCACATGG
    ACGGTTGATC GCCAACCCCA GTTTATCCAA GGCTACCGAG TGATGTATCG TCAGACTTCA
    GGTCTGCAGG CGACATCTTC GTGGCAGAAT TTAGATGCCA AAGTCCCGAC TGAACGAAGT
    GCTGTCTTAG TCAACCTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTACG GCCATATTTT
    AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACGGTTC GTACTACTGA AGAAGCCCCA
    AGTGCCCCAC CACAGTCTGT CACTGTACTG ACAGTTGGAA GCTACAATAG CACAAGTATT
    AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CACCAGAATG GAATTATCCA AGAATACAAG
    ATCTGGTGTC TAGGAAATGA AACGCGATTC CATATCAACA AAACTGTGGA TGCAGCCATT
    CGGTCCGTAA TAATTGGTGG ATTATTCCCA GGTATTCAAT ACCGGGTAGA GGTTGCAGCT
    AGTACCAGTG CAGGGGTTGG AGTAAAGAGT GAGCCACAGC CAATAATAAT CGGGAGACGC
    AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTGGTG
    AAGCAACCAG CCTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT
    AGCATATGGT TGTATTGGCG AAGAAAGAAG AGGAAGGGAC TCAGTAATTA TGCTGTTACG
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GTCCAGGTCT TCTCAATGCT
    GGTGATCCCA GCTATCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGCTT GCCAGTAAAT
    AATAGCAACA GTGGCCCAAA TGAGATTGGA AATTTTGGCC GTGGAGATGT GCTGCCACCA
    GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGTAGC
    ATTGACTTCA CTACCAAAAC CAGTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA
    TATGCCACGA CACAGATCTT GCATTCCAAC AGCATACATG AATTGGCTGT CGATCTGCCT
    GATCCACAAT GGAAAAGCTC AATTCAGCAA AAAACAGATC TGATGGGATT TGGTTATTCT
    CTACCTGATC AGAACAAAGG TAACAATGCC TTACTTTACA TTCCTGACTA CCGATTGGCT
    GAGGGATTGT CTAATAGAAT GCCACACAAC CAGTCTCAGG ATTTCAGCAC CACCAGCTCT
    CACAACAGCT CAGAAAGGAG TGGCAGTCTC TCAGGTGGGA AAGGTGGAAA AAAGAAGAAA
    AATAAAAACT CTTCTAAACC ACAGAAAAAC AATGGATCCA CTTGGGCCAA TGTCCCTCTA
    CCTCCCCCCC CAGTCCAGCC CCTTCCTGGC ACGGAGCTGG AACACTATGC AGTGGAACAA
    CAAGAAAATG GCTATGACAG TGATAGCTGG TGCCCACCAT TGCCAGTACA AACTTACTTA
    CACCAAGGTC TGGAAGATGA ACTGGAAGAA GATGATGATA GGGTCCCAAC ACCTCCTGTT
    CGAGGCGTGG CTTCTTCTCC TGCTATCTCC TTTGGACAGC AGTCCACTGC AACTCTTACT
    CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAGGCTC ACCTGGATGA GTTGACAAGA
    GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAA TCAACCTCCA
    CAGCCTCCAG TTCCACCGTT AGGTTATGTG TCTGGAGCCT TGATTTCTGA TTTGGAAACG
    GATGTTGCAG ATGATGATGC CGACGACGAA GAGGAAGCTT TAGAAATCCC CAGGCCCCTG
    AGAGCACTGG ACCAGACTCC TGGATCCAGC ATGGACAATC TAGACAGCTC TGTGACAGGT
    TCAATGGTAA ATGGATGGGG CTCTGCATCT GATGAGGATC GTAACTTTTC TAGTCATAGA
    TCTAGTGTAG GTAGCTCCTC AGATGGCTCT ATCTTTGCCA GCGGCAGTTT TGCACAAGCA
    CTGGTGGCAG CAGCAGATAA AGCTGGTTTT AGGCTGGATG GAACCAGCCT TACAAGAACA
    GGAAAAGCCT TTACCTCCTC TCAAAGACCT CGACCTACCA GCCCATTTTC TACTGACAGT
    AACACCAGTG CAGCCCTGAG TCAAAGTCAG AGGCCTCGGC CCACTAAAAA ACACAAGGGA
    GGGCGGATGG ACCAACAACC AGCATTGCCT CATCGAAGGG AAGGAATGAC AGATGATCTT
    CCACCACCAC CAGATCCCCC GCCAGGTCAG GGTTTAAGGC AGCAAATAGG CCCGAGCCAG
    CAGGCTGGTA ACGTGGAAAA CTCAGCAGAG AGAAAAGGAA GCTCTCTAGA GAGACAACAT
    GCATCCAGCT TAGAAGACAC AAAGAGCTCA TTGGATTGTC CAGCTAGAAC CTCCCTAGAG
    TGGCAGCGAC AAACCCAGGA ATGGATAAGC TCCACAGAAC GACAAGAAGA TATACGGAAA
    GCCCCACACA AACAAGGTGT CGGATCAGAG GAGGCCTTGG TGCCCTATAG CAAGCCCAGT
    TTCCCATCTC CAGGTGGCCA CAGCTCATCA GGAACAGCTT CTTCTAAGGG ATCCACTGGA
    CCTAGGAAAA CCGAGGTGTT GAGAGCAGGC CACCAGCGCA ATGCCAGCGA CCTTCTTGAC
    ATAGGATATA TGGGCTCCAA CAGTCAAGGA CAGTTTACAG GTGAATTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94461
    Clone ID Related Accession (Same CDS sequence) XM_017006987.1
    Accession Version XM_017006987.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4665bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product roundabout homolog 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
    CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT
    TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT
    CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC
    GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA
    AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC
    CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG
    TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA
    CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT
    CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC
    CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT
    GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT
    ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT
    AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC
    CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC
    ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG
    CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG
    GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT
    CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT
    GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG
    CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA
    GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG
    GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC
    GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT
    GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT
    CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG
    CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC
    GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT
    GGTTATTCTC TACCTGATCA GAACAAAGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA
    AACTCTTCTA AACCACAGAA AAACAATGGA TCCACTTGGG CCAATGTCCC TCTACCTCCC
    CCCCCAGTCC AGCCCCTTCC TGGCACGGAG CTGGAACACT ATGCAGTGGA ACAACAAGAA
    AATGGCTATG ACAGTGATAG CTGGTGCCCA CCATTGCCAG TACAAACTTA CTTACACCAA
    GGTCTGGAAG ATGAACTGGA AGAAGATGAT GATAGGGTCC CAACACCTCC TGTTCGAGGC
    GTGGCTTCTT CTCCTGCTAT CTCCTTTGGA CAGCAGTCCA CTGCAACTCT TACTCCATCC
    CCACGGGAAG AGATGCAACC CATGCTGCAG GCTCACCTGG ATGAGTTGAC AAGAGCCTAT
    CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATAATCAACC TCCACAGCCT
    CCAGTTCCAC CGTTAGGTTA TGTGTCTGGA GCCTTGATTT CTGATTTGGA AACGGATGTT
    GCAGATGATG ATGCCGACGA CGAAGAGGAA GCTTTAGAAA TCCCCAGGCC CCTGAGAGCA
    CTGGACCAGA CTCCTGGATC CAGCATGGAC AATCTAGACA GCTCTGTGAC AGGTTCAATG
    GTAAATGGAT GGGGCTCTGC ATCTGATGAG GATCGTAACT TTTCTAGTCA TAGATCTAGT
    GTAGGTAGCT CCTCAGATGG CTCTATCTTT GCCAGCGGCA GTTTTGCACA AGCACTGGTG
    GCAGCAGCAG ATAAAGCTGG TTTTAGGCTG GATGGAACCA GCCTTACAAG AACAGGAAAA
    GCCTTTACCT CCTCTCAAAG ACCTCGACCT ACCAGCCCAT TTTCTACTGA CAGTAACACC
    AGTGCAGCCC TGAGTCAAAG TCAGAGGCCT CGGCCCACTA AAAAACACAA GGGAGGGCGG
    ATGGACCAAC AACCAGCATT GCCTCATCGA AGGGAAGGAA TGACAGATGA TCTTCCACCA
    CCACCAGATC CCCCGCCAGG TCAGGGTTTA AGGCAGCAAA TAGGCCCGAG CCAGCAGGCT
    GGTAACGTGG AAAACTCAGC AGAGAGAAAA GGAAGCTCTC TAGAGAGACA ACATGCATCC
    AGCTTAGAAG ACACAAAGAG CTCATTGGAT TGTCCAGCTA GAACCTCCCT AGAGTGGCAG
    CGACAAACCC AGGAATGGAT AAGCTCCACA GAACGACAAG AAGATATACG GAAAGCCCCA
    CACAAACAAG GTGTCGGATC AGAGGAGGCC TTGGTGCCCT ATAGCAAGCC CAGTTTCCCA
    TCTCCAGGTG GCCACAGCTC ATCAGGAACA GCTTCTTCTA AGGGATCCAC TGGACCTAGG
    AAAACCGAGG TGTTGAGAGC AGGCCACCAG CGCAATGCCA GCGACCTTCT TGACATAGGA
    TATATGGGCT CCAACAGTCA AGGACAGTTT ACAGGTGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016862476.1
    CDS400..5064
    Translation

    Target ORF information:

    RefSeq Version XM_017006987.1
    Organism Homo sapiens(human)
    Definition Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017006987.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
    CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT
    TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT
    CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC
    GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA
    AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC
    CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG
    TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA
    CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT
    CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC
    CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT
    GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT
    ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT
    AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC
    CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC
    ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG
    CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG
    GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT
    CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT
    GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG
    CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA
    GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG
    GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC
    GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT
    GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT
    CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG
    CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC
    GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT
    GGTTATTCTC TACCTGATCA GAACAAAGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA
    AACTCTTCTA AACCACAGAA AAACAATGGA TCCACTTGGG CCAATGTCCC TCTACCTCCC
    CCCCCAGTCC AGCCCCTTCC TGGCACGGAG CTGGAACACT ATGCAGTGGA ACAACAAGAA
    AATGGCTATG ACAGTGATAG CTGGTGCCCA CCATTGCCAG TACAAACTTA CTTACACCAA
    GGTCTGGAAG ATGAACTGGA AGAAGATGAT GATAGGGTCC CAACACCTCC TGTTCGAGGC
    GTGGCTTCTT CTCCTGCTAT CTCCTTTGGA CAGCAGTCCA CTGCAACTCT TACTCCATCC
    CCACGGGAAG AGATGCAACC CATGCTGCAG GCTCACCTGG ATGAGTTGAC AAGAGCCTAT
    CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATAATCAACC TCCACAGCCT
    CCAGTTCCAC CGTTAGGTTA TGTGTCTGGA GCCTTGATTT CTGATTTGGA AACGGATGTT
    GCAGATGATG ATGCCGACGA CGAAGAGGAA GCTTTAGAAA TCCCCAGGCC CCTGAGAGCA
    CTGGACCAGA CTCCTGGATC CAGCATGGAC AATCTAGACA GCTCTGTGAC AGGTTCAATG
    GTAAATGGAT GGGGCTCTGC ATCTGATGAG GATCGTAACT TTTCTAGTCA TAGATCTAGT
    GTAGGTAGCT CCTCAGATGG CTCTATCTTT GCCAGCGGCA GTTTTGCACA AGCACTGGTG
    GCAGCAGCAG ATAAAGCTGG TTTTAGGCTG GATGGAACCA GCCTTACAAG AACAGGAAAA
    GCCTTTACCT CCTCTCAAAG ACCTCGACCT ACCAGCCCAT TTTCTACTGA CAGTAACACC
    AGTGCAGCCC TGAGTCAAAG TCAGAGGCCT CGGCCCACTA AAAAACACAA GGGAGGGCGG
    ATGGACCAAC AACCAGCATT GCCTCATCGA AGGGAAGGAA TGACAGATGA TCTTCCACCA
    CCACCAGATC CCCCGCCAGG TCAGGGTTTA AGGCAGCAAA TAGGCCCGAG CCAGCAGGCT
    GGTAACGTGG AAAACTCAGC AGAGAGAAAA GGAAGCTCTC TAGAGAGACA ACATGCATCC
    AGCTTAGAAG ACACAAAGAG CTCATTGGAT TGTCCAGCTA GAACCTCCCT AGAGTGGCAG
    CGACAAACCC AGGAATGGAT AAGCTCCACA GAACGACAAG AAGATATACG GAAAGCCCCA
    CACAAACAAG GTGTCGGATC AGAGGAGGCC TTGGTGCCCT ATAGCAAGCC CAGTTTCCCA
    TCTCCAGGTG GCCACAGCTC ATCAGGAACA GCTTCTTCTA AGGGATCCAC TGGACCTAGG
    AAAACCGAGG TGTTGAGAGC AGGCCACCAG CGCAATGCCA GCGACCTTCT TGACATAGGA
    TATATGGGCT CCAACAGTCA AGGACAGTTT ACAGGTGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94462
    Clone ID Related Accession (Same CDS sequence) XM_017006988.1
    Accession Version XM_017006988.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4611bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product roundabout homolog 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
    CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT
    TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT
    CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC
    GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA
    AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC
    CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG
    TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA
    CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT
    CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC
    CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT
    GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT
    ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT
    AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC
    CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC
    ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG
    CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG
    GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT
    CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT
    GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG
    CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA
    GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG
    GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC
    GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT
    GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT
    CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG
    CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC
    GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT
    GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGCCT TACTTTACAT TCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCTCAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA ATAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCCAC TTGGGCCAAT
    GTCCCTCTAC CTCCCCCCCC AGTCCAGCCC CTTCCTGGCA CGGAGCTGGA ACACTATGCA
    GTGGAACAAC AAGAAAATGG CTATGACAGT GATAGCTGGT GCCCACCATT GCCAGTACAA
    ACTTACTTAC ACCAAGGTCT GGAAGATGAA CTGGAAGAAG ATGATGATAG GGTCCCAACA
    CCTCCTGTTC GAGGCGTGGC TTCTTCTCCT GCTATCTCCT TTGGACAGCA GTCCACTGCA
    ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAGGCTCA CCTGGATGAG
    TTGACAAGAG CCTATCAGTT TGATATAGCA AAACAAACAT GGCACATTCA AAGCAATAAT
    CAACCTCCAC AGCCTCCAGT TCCACCGTTA GGTTATGTGT CTGGAGCCTT GATTTCTGAT
    TTGGAAACGG ATGTTGCAGA TGATGATGCC GACGACGAAG AGGAAGCTTT AGAAATCCCC
    AGGCCCCTGA GAGCACTGGA CCAGACTCCT GGATCCAGCA TGGACAATCT AGACAGCTCT
    GTGACAGGAA AAGCCTTTAC CTCCTCTCAA AGACCTCGAC CTACCAGCCC ATTTTCTACT
    GACAGTAACA CCAGTGCAGC CCTGAGTCAA AGTCAGAGGC CTCGGCCCAC TAAAAAACAC
    AAGGGAGGGC GGATGGACCA ACAACCAGCA TTGCCTCATC GAAGGGAAGG AATGACAGAT
    GATCTTCCAC CACCACCAGA TCCCCCGCCA GGTCAGGGTT TAAGGCAGCA AATAGGCCCG
    AGCCAGCAGG CTGGTAACGT GGAAAACTCA GCAGAGAGAA AAGGAAGCTC TCTAGAGAGA
    CAACATGCAT CCAGCTTAGA AGACACAAAG AGCTCATTGG ATTGTCCAGC TAGAACCTCC
    CTAGAGTGGC AGCGACAAAC CCAGGAATGG ATAAGCTCCA CAGAACGACA AGAAGATATA
    CGGAAAGCCC CACACAAACA AGGTGTCGGA TCAGAGGAGG CCTTGGTGCC CTATAGCAAG
    CCCAGTTTCC CATCTCCAGG TGGCCACAGC TCATCAGGAA CAGCTTCTTC TAAGGGATCC
    ACTGGACCTA GGAAAACCGA GGTGTTGAGA GCAGGCCACC AGCGCAATGC CAGCGACCTT
    CTTGACATAG GATATATGGG CTCCAACAGT CAAGGACAGT TTACAGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016862477.1
    CDS400..5010
    Translation

    Target ORF information:

    RefSeq Version XM_017006988.1
    Organism Homo sapiens(human)
    Definition Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017006988.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
    CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT
    TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT
    CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC
    GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA
    AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC
    CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG
    TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA
    CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT
    CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC
    CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT
    GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT
    ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT
    AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC
    CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC
    ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG
    CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG
    GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT
    CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT
    GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG
    CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA
    GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG
    GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC
    GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT
    GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT
    CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG
    CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC
    GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT
    GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGCCT TACTTTACAT TCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCTCAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA ATAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCCAC TTGGGCCAAT
    GTCCCTCTAC CTCCCCCCCC AGTCCAGCCC CTTCCTGGCA CGGAGCTGGA ACACTATGCA
    GTGGAACAAC AAGAAAATGG CTATGACAGT GATAGCTGGT GCCCACCATT GCCAGTACAA
    ACTTACTTAC ACCAAGGTCT GGAAGATGAA CTGGAAGAAG ATGATGATAG GGTCCCAACA
    CCTCCTGTTC GAGGCGTGGC TTCTTCTCCT GCTATCTCCT TTGGACAGCA GTCCACTGCA
    ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAGGCTCA CCTGGATGAG
    TTGACAAGAG CCTATCAGTT TGATATAGCA AAACAAACAT GGCACATTCA AAGCAATAAT
    CAACCTCCAC AGCCTCCAGT TCCACCGTTA GGTTATGTGT CTGGAGCCTT GATTTCTGAT
    TTGGAAACGG ATGTTGCAGA TGATGATGCC GACGACGAAG AGGAAGCTTT AGAAATCCCC
    AGGCCCCTGA GAGCACTGGA CCAGACTCCT GGATCCAGCA TGGACAATCT AGACAGCTCT
    GTGACAGGAA AAGCCTTTAC CTCCTCTCAA AGACCTCGAC CTACCAGCCC ATTTTCTACT
    GACAGTAACA CCAGTGCAGC CCTGAGTCAA AGTCAGAGGC CTCGGCCCAC TAAAAAACAC
    AAGGGAGGGC GGATGGACCA ACAACCAGCA TTGCCTCATC GAAGGGAAGG AATGACAGAT
    GATCTTCCAC CACCACCAGA TCCCCCGCCA GGTCAGGGTT TAAGGCAGCA AATAGGCCCG
    AGCCAGCAGG CTGGTAACGT GGAAAACTCA GCAGAGAGAA AAGGAAGCTC TCTAGAGAGA
    CAACATGCAT CCAGCTTAGA AGACACAAAG AGCTCATTGG ATTGTCCAGC TAGAACCTCC
    CTAGAGTGGC AGCGACAAAC CCAGGAATGG ATAAGCTCCA CAGAACGACA AGAAGATATA
    CGGAAAGCCC CACACAAACA AGGTGTCGGA TCAGAGGAGG CCTTGGTGCC CTATAGCAAG
    CCCAGTTTCC CATCTCCAGG TGGCCACAGC TCATCAGGAA CAGCTTCTTC TAAGGGATCC
    ACTGGACCTA GGAAAACCGA GGTGTTGAGA GCAGGCCACC AGCGCAATGC CAGCGACCTT
    CTTGACATAG GATATATGGG CTCCAACAGT CAAGGACAGT TTACAGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94463
    Clone ID Related Accession (Same CDS sequence) XM_017006989.1
    Accession Version XM_017006989.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4605bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product roundabout homolog 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
    CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT
    TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT
    CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC
    GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA
    AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC
    CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG
    TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA
    CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT
    CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCCCCC ACAGTTTGTG
    GTTCGGCCAA GAGATCAGAT TGTTGCTCAA GGTCGAACAG TGACATTTCC CTGTGAAACT
    AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAGAAAGAAG GCAGCCAGAA CCTACTTTTC
    CCAAACCAAC CCCAGCAGCC CAACAGTAGA TGCTCAGTGT CACCAACTGG AGACCTCACA
    ATCACCAACA TTCAACGTTC CGACGCGGGT TACTACATCT GCCAGGCTTT AACTGTGGCA
    GGAAGCATTT TAGCAAAAGC TCAACTGGAG GTTACTGATG TTTTGACAGA TAGACCTCCA
    CCTATAATTC TACAAGGCCC AGCCAACCAA ACGCTGGCAG TGGATGGTAC AGCGTTACTG
    AAATGTAAAG CCACTGGTGA TCCTCTTCCT GTAATTAGCT GGTTAAAGGA GGGATTTACT
    TTTCCGGGTA GAGATCCAAG AGCAACAATT CAAGAGCAAG GCACACTGCA GATTAAGAAT
    TTACGGATTT CTGATACTGG CACTTATACT TGTGTGGCTA CAAGTTCAAG TGGAGAGACT
    TCCTGGAGTG CAGTGCTGGA TGTGACAGAG TCTGGAGCAA CAATCAGTAA AAACTATGAT
    TTAAGTGACC TGCCAGGGCC ACCATCCAAA CCGCAGGTCA CTGATGTTAC TAAGAACAGT
    GTCACCTTGT CCTGGCAGCC AGGTACCCCT GGAACCCTTC CAGCAAGTGC ATATATCATT
    GAGGCTTTCA GCCAATCAGT GAGCAACAGC TGGCAGACCG TGGCAAACCA TGTAAAGACC
    ACCCTCTATA CTGTAAGAGG ACTGCGGCCC AATACAATCT ACTTATTCAT GGTCAGAGCG
    ATCAACCCCC AAGGTCTCAG TGACCCAAGT CCCATGTCAG ATCCTGTGCG CACACAAGAT
    ATCAGCCCAC CAGCACAAGG AGTGGACCAC AGGCAAGTGC AGAAAGAGCT AGGAGATGTC
    CTTGTCCGTC TTCATAATCC AGTTGTGCTG ACTCCCACCA CGGTTCAGGT CACATGGACG
    GTTGATCGCC AACCCCAGTT TATCCAAGGC TACCGAGTGA TGTATCGTCA GACTTCAGGT
    CTGCAGGCGA CATCTTCGTG GCAGAATTTA GATGCCAAAG TCCCGACTGA ACGAAGTGCT
    GTCTTAGTCA ACCTGAAAAA GGGGGTGACT TATGAAATTA AAGTACGGCC ATATTTTAAT
    GAGTTCCAAG GAATGGATAG TGAATCTAAA ACGGTTCGTA CTACTGAAGA AGCCCCAAGT
    GCCCCACCAC AGTCTGTCAC TGTACTGACA GTTGGAAGCT ACAATAGCAC AAGTATTAGT
    GTTTCCTGGG ATCCTCCTCC TCCAGATCAC CAGAATGGAA TTATCCAAGA ATACAAGATC
    TGGTGTCTAG GAAATGAAAC GCGATTCCAT ATCAACAAAA CTGTGGATGC AGCCATTCGG
    TCCGTAATAA TTGGTGGATT ATTCCCAGGT ATTCAATACC GGGTAGAGGT TGCAGCTAGT
    ACCAGTGCAG GGGTTGGAGT AAAGAGTGAG CCACAGCCAA TAATAATCGG GAGACGCAAT
    GAAGTTGTCA TTACTGAAAA CAATAACAGC ATAACTGAGC AAATCACTGA TGTGGTGAAG
    CAACCAGCCT TTATAGCTGG TATTGGTGGT GCCTGCTGGG TAATTCTGAT GGGTTTTAGC
    ATATGGTTGT ATTGGCGAAG AAAGAAGAGG AAGGGACTCA GTAATTATGC TGTTACGTTT
    CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGAAGCCGTC CAGGTCTTCT CAATGCTGGT
    GATCCCAGCT ATCCATGGCT TGCTGATTCT TGGCCAGCCA CGAGCTTGCC AGTAAATAAT
    AGCAACAGTG GCCCAAATGA GATTGGAAAT TTTGGCCGTG GAGATGTGCT GCCACCAGTT
    CCAGGCCAAG GGGATAAAAC AGCAACGATG CTCTCAGATG GAGCCATTTA TAGTAGCATT
    GACTTCACTA CCAAAACCAG TTACAACAGT TCCAGCCAAA TAACACAGGC TACCCCATAT
    GCCACGACAC AGATCTTGCA TTCCAACAGC ATACATGAAT TGGCTGTCGA TCTGCCTGAT
    CCACAATGGA AAAGCTCAAT TCAGCAAAAA ACAGATCTGA TGGGATTTGG TTATTCTCTA
    CCTGATCAGA ACAAAGGTAA CAATGCCTTA CTTTACATTC CTGACTACCG ATTGGCTGAG
    GGATTGTCTA ATAGAATGCC ACACAACCAG TCTCAGGATT TCAGCACCAC CAGCTCTCAC
    AACAGCTCAG AAAGGAGTGG CAGTCTCTCA GGTGGGAAAG GTGGAAAAAA GAAGAAAAAT
    AAAAACTCTT CTAAACCACA GAAAAACAAT GGATCCACTT GGGCCAATGT CCCTCTACCT
    CCCCCCCCAG TCCAGCCCCT TCCTGGCACG GAGCTGGAAC ACTATGCAGT GGAACAACAA
    GAAAATGGCT ATGACAGTGA TAGCTGGTGC CCACCATTGC CAGTACAAAC TTACTTACAC
    CAAGGTCTGG AAGATGAACT GGAAGAAGAT GATGATAGGG TCCCAACACC TCCTGTTCGA
    GGCGTGGCTT CTTCTCCTGC TATCTCCTTT GGACAGCAGT CCACTGCAAC TCTTACTCCA
    TCCCCACGGG AAGAGATGCA ACCCATGCTG CAGGCTCACC TGGATGAGTT GACAAGAGCC
    TATCAGTTTG ATATAGCAAA ACAAACATGG CACATTCAAA GCAATAATCA ACCTCCACAG
    CCTCCAGTTC CACCGTTAGG TTATGTGTCT GGAGCCTTGA TTTCTGATTT GGAAACGGAT
    GTTGCAGATG ATGATGCCGA CGACGAAGAG GAAGCTTTAG AAATCCCCAG GCCCCTGAGA
    GCACTGGACC AGACTCCTGG ATCCAGCATG GACAATCTAG ACAGCTCTGT GACAGGAAAA
    GCCTTTACCT CCTCTCAAAG ACCTCGACCT ACCAGCCCAT TTTCTACTGA CAGTAACACC
    AGTGCAGCCC TGAGTCAAAG TCAGAGGCCT CGGCCCACTA AAAAACACAA GGGAGGGCGG
    ATGGACCAAC AACCAGCATT GCCTCATCGA AGGGAAGGAA TGACAGATGA TCTTCCACCA
    CCACCAGATC CCCCGCCAGG TCAGGGTTTA AGGCAGCAAA TAGGCCCGAG CCAGCAGGCT
    GGTAACGTGG AAAACTCAGC AGAGAGAAAA GGAAGCTCTC TAGAGAGACA ACATGCATCC
    AGCTTAGAAG ACACAAAGAG CTCATTGGAT TGTCCAGCTA GAACCTCCCT AGAGTGGCAG
    CGACAAACCC AGGAATGGAT AAGCTCCACA GAACGACAAG AAGATATACG GAAAGCCCCA
    CACAAACAAG GTGTCGGATC AGAGGAGGCC TTGGTGCCCT ATAGCAAGCC CAGTTTCCCA
    TCTCCAGGTG GCCACAGCTC ATCAGGAACA GCTTCTTCTA AGGGATCCAC TGGACCTAGG
    AAAACCGAGG TGTTGAGAGC AGGCCACCAG CGCAATGCCA GCGACCTTCT TGACATAGGA
    TATATGGGCT CCAACAGTCA AGGACAGTTT ACAGGTGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016862478.1
    CDS400..5004
    Translation

    Target ORF information:

    RefSeq Version XM_017006989.1
    Organism Homo sapiens(human)
    Definition Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017006989.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
    CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT
    TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT
    CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC
    GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA
    AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC
    CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG
    TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA
    CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT
    CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCCCCC ACAGTTTGTG
    GTTCGGCCAA GAGATCAGAT TGTTGCTCAA GGTCGAACAG TGACATTTCC CTGTGAAACT
    AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAGAAAGAAG GCAGCCAGAA CCTACTTTTC
    CCAAACCAAC CCCAGCAGCC CAACAGTAGA TGCTCAGTGT CACCAACTGG AGACCTCACA
    ATCACCAACA TTCAACGTTC CGACGCGGGT TACTACATCT GCCAGGCTTT AACTGTGGCA
    GGAAGCATTT TAGCAAAAGC TCAACTGGAG GTTACTGATG TTTTGACAGA TAGACCTCCA
    CCTATAATTC TACAAGGCCC AGCCAACCAA ACGCTGGCAG TGGATGGTAC AGCGTTACTG
    AAATGTAAAG CCACTGGTGA TCCTCTTCCT GTAATTAGCT GGTTAAAGGA GGGATTTACT
    TTTCCGGGTA GAGATCCAAG AGCAACAATT CAAGAGCAAG GCACACTGCA GATTAAGAAT
    TTACGGATTT CTGATACTGG CACTTATACT TGTGTGGCTA CAAGTTCAAG TGGAGAGACT
    TCCTGGAGTG CAGTGCTGGA TGTGACAGAG TCTGGAGCAA CAATCAGTAA AAACTATGAT
    TTAAGTGACC TGCCAGGGCC ACCATCCAAA CCGCAGGTCA CTGATGTTAC TAAGAACAGT
    GTCACCTTGT CCTGGCAGCC AGGTACCCCT GGAACCCTTC CAGCAAGTGC ATATATCATT
    GAGGCTTTCA GCCAATCAGT GAGCAACAGC TGGCAGACCG TGGCAAACCA TGTAAAGACC
    ACCCTCTATA CTGTAAGAGG ACTGCGGCCC AATACAATCT ACTTATTCAT GGTCAGAGCG
    ATCAACCCCC AAGGTCTCAG TGACCCAAGT CCCATGTCAG ATCCTGTGCG CACACAAGAT
    ATCAGCCCAC CAGCACAAGG AGTGGACCAC AGGCAAGTGC AGAAAGAGCT AGGAGATGTC
    CTTGTCCGTC TTCATAATCC AGTTGTGCTG ACTCCCACCA CGGTTCAGGT CACATGGACG
    GTTGATCGCC AACCCCAGTT TATCCAAGGC TACCGAGTGA TGTATCGTCA GACTTCAGGT
    CTGCAGGCGA CATCTTCGTG GCAGAATTTA GATGCCAAAG TCCCGACTGA ACGAAGTGCT
    GTCTTAGTCA ACCTGAAAAA GGGGGTGACT TATGAAATTA AAGTACGGCC ATATTTTAAT
    GAGTTCCAAG GAATGGATAG TGAATCTAAA ACGGTTCGTA CTACTGAAGA AGCCCCAAGT
    GCCCCACCAC AGTCTGTCAC TGTACTGACA GTTGGAAGCT ACAATAGCAC AAGTATTAGT
    GTTTCCTGGG ATCCTCCTCC TCCAGATCAC CAGAATGGAA TTATCCAAGA ATACAAGATC
    TGGTGTCTAG GAAATGAAAC GCGATTCCAT ATCAACAAAA CTGTGGATGC AGCCATTCGG
    TCCGTAATAA TTGGTGGATT ATTCCCAGGT ATTCAATACC GGGTAGAGGT TGCAGCTAGT
    ACCAGTGCAG GGGTTGGAGT AAAGAGTGAG CCACAGCCAA TAATAATCGG GAGACGCAAT
    GAAGTTGTCA TTACTGAAAA CAATAACAGC ATAACTGAGC AAATCACTGA TGTGGTGAAG
    CAACCAGCCT TTATAGCTGG TATTGGTGGT GCCTGCTGGG TAATTCTGAT GGGTTTTAGC
    ATATGGTTGT ATTGGCGAAG AAAGAAGAGG AAGGGACTCA GTAATTATGC TGTTACGTTT
    CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGAAGCCGTC CAGGTCTTCT CAATGCTGGT
    GATCCCAGCT ATCCATGGCT TGCTGATTCT TGGCCAGCCA CGAGCTTGCC AGTAAATAAT
    AGCAACAGTG GCCCAAATGA GATTGGAAAT TTTGGCCGTG GAGATGTGCT GCCACCAGTT
    CCAGGCCAAG GGGATAAAAC AGCAACGATG CTCTCAGATG GAGCCATTTA TAGTAGCATT
    GACTTCACTA CCAAAACCAG TTACAACAGT TCCAGCCAAA TAACACAGGC TACCCCATAT
    GCCACGACAC AGATCTTGCA TTCCAACAGC ATACATGAAT TGGCTGTCGA TCTGCCTGAT
    CCACAATGGA AAAGCTCAAT TCAGCAAAAA ACAGATCTGA TGGGATTTGG TTATTCTCTA
    CCTGATCAGA ACAAAGGTAA CAATGCCTTA CTTTACATTC CTGACTACCG ATTGGCTGAG
    GGATTGTCTA ATAGAATGCC ACACAACCAG TCTCAGGATT TCAGCACCAC CAGCTCTCAC
    AACAGCTCAG AAAGGAGTGG CAGTCTCTCA GGTGGGAAAG GTGGAAAAAA GAAGAAAAAT
    AAAAACTCTT CTAAACCACA GAAAAACAAT GGATCCACTT GGGCCAATGT CCCTCTACCT
    CCCCCCCCAG TCCAGCCCCT TCCTGGCACG GAGCTGGAAC ACTATGCAGT GGAACAACAA
    GAAAATGGCT ATGACAGTGA TAGCTGGTGC CCACCATTGC CAGTACAAAC TTACTTACAC
    CAAGGTCTGG AAGATGAACT GGAAGAAGAT GATGATAGGG TCCCAACACC TCCTGTTCGA
    GGCGTGGCTT CTTCTCCTGC TATCTCCTTT GGACAGCAGT CCACTGCAAC TCTTACTCCA
    TCCCCACGGG AAGAGATGCA ACCCATGCTG CAGGCTCACC TGGATGAGTT GACAAGAGCC
    TATCAGTTTG ATATAGCAAA ACAAACATGG CACATTCAAA GCAATAATCA ACCTCCACAG
    CCTCCAGTTC CACCGTTAGG TTATGTGTCT GGAGCCTTGA TTTCTGATTT GGAAACGGAT
    GTTGCAGATG ATGATGCCGA CGACGAAGAG GAAGCTTTAG AAATCCCCAG GCCCCTGAGA
    GCACTGGACC AGACTCCTGG ATCCAGCATG GACAATCTAG ACAGCTCTGT GACAGGAAAA
    GCCTTTACCT CCTCTCAAAG ACCTCGACCT ACCAGCCCAT TTTCTACTGA CAGTAACACC
    AGTGCAGCCC TGAGTCAAAG TCAGAGGCCT CGGCCCACTA AAAAACACAA GGGAGGGCGG
    ATGGACCAAC AACCAGCATT GCCTCATCGA AGGGAAGGAA TGACAGATGA TCTTCCACCA
    CCACCAGATC CCCCGCCAGG TCAGGGTTTA AGGCAGCAAA TAGGCCCGAG CCAGCAGGCT
    GGTAACGTGG AAAACTCAGC AGAGAGAAAA GGAAGCTCTC TAGAGAGACA ACATGCATCC
    AGCTTAGAAG ACACAAAGAG CTCATTGGAT TGTCCAGCTA GAACCTCCCT AGAGTGGCAG
    CGACAAACCC AGGAATGGAT AAGCTCCACA GAACGACAAG AAGATATACG GAAAGCCCCA
    CACAAACAAG GTGTCGGATC AGAGGAGGCC TTGGTGCCCT ATAGCAAGCC CAGTTTCCCA
    TCTCCAGGTG GCCACAGCTC ATCAGGAACA GCTTCTTCTA AGGGATCCAC TGGACCTAGG
    AAAACCGAGG TGTTGAGAGC AGGCCACCAG CGCAATGCCA GCGACCTTCT TGACATAGGA
    TATATGGGCT CCAACAGTCA AGGACAGTTT ACAGGTGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94464
    Clone ID Related Accession (Same CDS sequence) XM_017006990.1
    Accession Version XM_017006990.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4596bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product roundabout homolog 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCCAGAA GACATGAACG TGTCACTAGA AGGATGTGGA CATGGGCTCC GGGACTGTTG 
    ATGATGACTG TGGTGTTTTG GGGTCATCAG GGGAATGGAC AAGGCCAAGG ATCGCGTCTT
    CGCCAGGAGG ACTTTCCCCC GCGGATTGTG GAGCATCCTT CCGATGTCAT CGTCTCTAAG
    GGCGAGCCCA CGACTCTGAA CTGCAAGGCG GAGGGCCGGC CAACGCCCAC CATTGAGTGG
    TACAAAGATG GGGAGCGAGT GGAGACTGAC AAGGACGATC CCCGGTCCCA CAGGATGCTT
    CTGCCCAGCG GATCCTTATT CTTCTTGCGC ATCGTGCACG GGCGCAGGAG TAAACCTGAT
    GAAGGAAGCT ACGTTTGTGT TGCGAGGAAC TATCTTGGTG AAGCAGTGAG TCGAAATGCG
    TCTCTGGAAG TGGCATTGTT ACGAGATGAC TTCCGACAAA ACCCCACAGA TGTTGTAGTG
    GCAGCTGGAG AGCCTGCAAT CCTGGAGTGC CAGCCTCCCC GGGGACACCC AGAACCCACC
    ATCTACTGGA AAAAAGACAA AGTTCGAATT GATGACAAGG AAGAAAGAAT AAGTATCCGT
    GGTGGAAAAC TGATGATCTC CAATACCAGG AAAAGTGATG CAGGGATGTA TACTTGTGTT
    GGTACCAATA TGGTGGGAGA AAGGGACAGT GACCCAGCAG AGCTGACTGT CTTTGAACGA
    CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTACTGG AGGAAGAAGC TGTAGAATTT
    CGTTGTCAAG TCCAAGGAGA TCCTCAACCA ACTGTGAGGT GGAAAAAGGA TGATGCAGAC
    TTGCCAAGAG GAAGGTATGA CATCAAAGAC GATTACACAC TAAGAATTAA AAAGACCATG
    AGTACAGATG AAGGCACCTA TATGTGTATT GCTGAGAATC GGGTTGGAAA AATGGAAGCC
    TCTGCTACAC TCACCGTCCG AGCTCGCCCT GTTGCTCCCC CACAGTTTGT GGTTCGGCCA
    AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC
    CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA
    CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC
    ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT
    TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT
    CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA
    GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT
    AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT
    TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT
    GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC
    CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG
    TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC
    AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT
    ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC
    CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA
    CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT
    CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC
    CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG
    ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC
    AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA
    GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA
    CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG
    GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA
    GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA
    ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA
    GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC
    ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC
    TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG
    TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA
    GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC
    TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT
    GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA
    GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT
    ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA
    CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG
    AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG
    AACAAAGGTA ACAATGCCTT ACTTTACATT CCTGACTACC GATTGGCTGA GGGATTGTCT
    AATAGAATGC CACACAACCA GTCTCAGGAT TTCAGCACCA CCAGCTCTCA CAACAGCTCA
    GAAAGGAGTG GCAGTCTCTC AGGTGGGAAA GGTGGAAAAA AGAAGAAAAA TAAAAACTCT
    TCTAAACCAC AGAAAAACAA TGGATCCACT TGGGCCAATG TCCCTCTACC TCCCCCCCCA
    GTCCAGCCCC TTCCTGGCAC GGAGCTGGAA CACTATGCAG TGGAACAACA AGAAAATGGC
    TATGACAGTG ATAGCTGGTG CCCACCATTG CCAGTACAAA CTTACTTACA CCAAGGTCTG
    GAAGATGAAC TGGAAGAAGA TGATGATAGG GTCCCAACAC CTCCTGTTCG AGGCGTGGCT
    TCTTCTCCTG CTATCTCCTT TGGACAGCAG TCCACTGCAA CTCTTACTCC ATCCCCACGG
    GAAGAGATGC AACCCATGCT GCAGGCTCAC CTGGATGAGT TGACAAGAGC CTATCAGTTT
    GATATAGCAA AACAAACATG GCACATTCAA AGCAATAATC AACCTCCACA GCCTCCAGTT
    CCACCGTTAG GTTATGTGTC TGGAGCCTTG ATTTCTGATT TGGAAACGGA TGTTGCAGAT
    GATGATGCCG ACGACGAAGA GGAAGCTTTA GAAATCCCCA GGCCCCTGAG AGCACTGGAC
    CAGACTCCTG GATCCAGCAT GGACAATCTA GACAGCTCTG TGACAGGAAA AGCCTTTACC
    TCCTCTCAAA GACCTCGACC TACCAGCCCA TTTTCTACTG ACAGTAACAC CAGTGCAGCC
    CTGAGTCAAA GTCAGAGGCC TCGGCCCACT AAAAAACACA AGGGAGGGCG GATGGACCAA
    CAACCAGCAT TGCCTCATCG AAGGGAAGGA ATGACAGATG ATCTTCCACC ACCACCAGAT
    CCCCCGCCAG GTCAGGGTTT AAGGCAGCAA ATAGGCCCGA GCCAGCAGGC TGGTAACGTG
    GAAAACTCAG CAGAGAGAAA AGGAAGCTCT CTAGAGAGAC AACATGCATC CAGCTTAGAA
    GACACAAAGA GCTCATTGGA TTGTCCAGCT AGAACCTCCC TAGAGTGGCA GCGACAAACC
    CAGGAATGGA TAAGCTCCAC AGAACGACAA GAAGATATAC GGAAAGCCCC ACACAAACAA
    GGTGTCGGAT CAGAGGAGGC CTTGGTGCCC TATAGCAAGC CCAGTTTCCC ATCTCCAGGT
    GGCCACAGCT CATCAGGAAC AGCTTCTTCT AAGGGATCCA CTGGACCTAG GAAAACCGAG
    GTGTTGAGAG CAGGCCACCA GCGCAATGCC AGCGACCTTC TTGACATAGG ATATATGGGC
    TCCAACAGTC AAGGACAGTT TACAGGTGAA TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016862479.1
    CDS300..4895
    Translation

    Target ORF information:

    RefSeq Version XM_017006990.1
    Organism Homo sapiens(human)
    Definition Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017006990.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCCAGAA GACATGAACG TGTCACTAGA AGGATGTGGA CATGGGCTCC GGGACTGTTG 
    ATGATGACTG TGGTGTTTTG GGGTCATCAG GGGAATGGAC AAGGCCAAGG ATCGCGTCTT
    CGCCAGGAGG ACTTTCCCCC GCGGATTGTG GAGCATCCTT CCGATGTCAT CGTCTCTAAG
    GGCGAGCCCA CGACTCTGAA CTGCAAGGCG GAGGGCCGGC CAACGCCCAC CATTGAGTGG
    TACAAAGATG GGGAGCGAGT GGAGACTGAC AAGGACGATC CCCGGTCCCA CAGGATGCTT
    CTGCCCAGCG GATCCTTATT CTTCTTGCGC ATCGTGCACG GGCGCAGGAG TAAACCTGAT
    GAAGGAAGCT ACGTTTGTGT TGCGAGGAAC TATCTTGGTG AAGCAGTGAG TCGAAATGCG
    TCTCTGGAAG TGGCATTGTT ACGAGATGAC TTCCGACAAA ACCCCACAGA TGTTGTAGTG
    GCAGCTGGAG AGCCTGCAAT CCTGGAGTGC CAGCCTCCCC GGGGACACCC AGAACCCACC
    ATCTACTGGA AAAAAGACAA AGTTCGAATT GATGACAAGG AAGAAAGAAT AAGTATCCGT
    GGTGGAAAAC TGATGATCTC CAATACCAGG AAAAGTGATG CAGGGATGTA TACTTGTGTT
    GGTACCAATA TGGTGGGAGA AAGGGACAGT GACCCAGCAG AGCTGACTGT CTTTGAACGA
    CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTACTGG AGGAAGAAGC TGTAGAATTT
    CGTTGTCAAG TCCAAGGAGA TCCTCAACCA ACTGTGAGGT GGAAAAAGGA TGATGCAGAC
    TTGCCAAGAG GAAGGTATGA CATCAAAGAC GATTACACAC TAAGAATTAA AAAGACCATG
    AGTACAGATG AAGGCACCTA TATGTGTATT GCTGAGAATC GGGTTGGAAA AATGGAAGCC
    TCTGCTACAC TCACCGTCCG AGCTCGCCCT GTTGCTCCCC CACAGTTTGT GGTTCGGCCA
    AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC
    CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA
    CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC
    ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT
    TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT
    CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA
    GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT
    AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT
    TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT
    GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC
    CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG
    TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC
    AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT
    ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC
    CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA
    CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT
    CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC
    CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG
    ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC
    AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA
    GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA
    CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG
    GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA
    GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA
    ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA
    GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC
    ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC
    TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG
    TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA
    GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC
    TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT
    GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA
    GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT
    ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA
    CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG
    AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG
    AACAAAGGTA ACAATGCCTT ACTTTACATT CCTGACTACC GATTGGCTGA GGGATTGTCT
    AATAGAATGC CACACAACCA GTCTCAGGAT TTCAGCACCA CCAGCTCTCA CAACAGCTCA
    GAAAGGAGTG GCAGTCTCTC AGGTGGGAAA GGTGGAAAAA AGAAGAAAAA TAAAAACTCT
    TCTAAACCAC AGAAAAACAA TGGATCCACT TGGGCCAATG TCCCTCTACC TCCCCCCCCA
    GTCCAGCCCC TTCCTGGCAC GGAGCTGGAA CACTATGCAG TGGAACAACA AGAAAATGGC
    TATGACAGTG ATAGCTGGTG CCCACCATTG CCAGTACAAA CTTACTTACA CCAAGGTCTG
    GAAGATGAAC TGGAAGAAGA TGATGATAGG GTCCCAACAC CTCCTGTTCG AGGCGTGGCT
    TCTTCTCCTG CTATCTCCTT TGGACAGCAG TCCACTGCAA CTCTTACTCC ATCCCCACGG
    GAAGAGATGC AACCCATGCT GCAGGCTCAC CTGGATGAGT TGACAAGAGC CTATCAGTTT
    GATATAGCAA AACAAACATG GCACATTCAA AGCAATAATC AACCTCCACA GCCTCCAGTT
    CCACCGTTAG GTTATGTGTC TGGAGCCTTG ATTTCTGATT TGGAAACGGA TGTTGCAGAT
    GATGATGCCG ACGACGAAGA GGAAGCTTTA GAAATCCCCA GGCCCCTGAG AGCACTGGAC
    CAGACTCCTG GATCCAGCAT GGACAATCTA GACAGCTCTG TGACAGGAAA AGCCTTTACC
    TCCTCTCAAA GACCTCGACC TACCAGCCCA TTTTCTACTG ACAGTAACAC CAGTGCAGCC
    CTGAGTCAAA GTCAGAGGCC TCGGCCCACT AAAAAACACA AGGGAGGGCG GATGGACCAA
    CAACCAGCAT TGCCTCATCG AAGGGAAGGA ATGACAGATG ATCTTCCACC ACCACCAGAT
    CCCCCGCCAG GTCAGGGTTT AAGGCAGCAA ATAGGCCCGA GCCAGCAGGC TGGTAACGTG
    GAAAACTCAG CAGAGAGAAA AGGAAGCTCT CTAGAGAGAC AACATGCATC CAGCTTAGAA
    GACACAAAGA GCTCATTGGA TTGTCCAGCT AGAACCTCCC TAGAGTGGCA GCGACAAACC
    CAGGAATGGA TAAGCTCCAC AGAACGACAA GAAGATATAC GGAAAGCCCC ACACAAACAA
    GGTGTCGGAT CAGAGGAGGC CTTGGTGCCC TATAGCAAGC CCAGTTTCCC ATCTCCAGGT
    GGCCACAGCT CATCAGGAAC AGCTTCTTCT AAGGGATCCA CTGGACCTAG GAAAACCGAG
    GTGTTGAGAG CAGGCCACCA GCGCAATGCC AGCGACCTTC TTGACATAGG ATATATGGGC
    TCCAACAGTC AAGGACAGTT TACAGGTGAA TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu11403
    Clone ID Related Accession (Same CDS sequence) NM_002942.4 , NM_002942.5
    Accession Version NM_002942.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4137bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-21
    Organism Homo sapiens(human)
    Product roundabout homolog 2 isoform ROBO2b precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ533874.1 and AC133040.3. On Jun 23, 2010 this sequence version replaced NM_002942.3. Transcript Variant: This variant (2) lacks two alternate in-frame exons in the coding region compared to variant 4. The encoded protein (isoform ROBO2b; PMID 16829019) is shorter than variant 4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ533874.1, BC146772.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154665, SAMEA2157437 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC
    ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC
    ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC
    CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG
    AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA
    GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG
    TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA
    GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG
    GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT
    AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA
    AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCCCC CACAGTTTGT GGTTCGGCCA
    AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC
    CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA
    CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC
    ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT
    TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT
    CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA
    GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT
    AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT
    TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT
    GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC
    CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG
    TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC
    AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT
    ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC
    CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA
    CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT
    CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC
    CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG
    ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC
    AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA
    GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA
    CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG
    GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA
    GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA
    ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA
    GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC
    ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC
    TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG
    TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA
    GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC
    TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT
    GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA
    GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT
    ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA
    CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG
    AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG
    AACAAAGGTA ACAATGGTGG GAAAGGTGGA AAAAAGAAGA AAAATAAAAA CTCTTCTAAA
    CCACAGAAAA ACAATGGATC CACTTGGGCC AATGTCCCTC TACCTCCCCC CCCAGTCCAG
    CCCCTTCCTG GCACGGAGCT GGAACACTAT GCAGTGGAAC AACAAGAAAA TGGCTATGAC
    AGTGATAGCT GGTGCCCACC ATTGCCAGTA CAAACTTACT TACACCAAGG TCTGGAAGAT
    GAACTGGAAG AAGATGATGA TAGGGTCCCA ACACCTCCTG TTCGAGGCGT GGCTTCTTCT
    CCTGCTATCT CCTTTGGACA GCAGTCCACT GCAACTCTTA CTCCATCCCC ACGGGAAGAG
    ATGCAACCCA TGCTGCAGGC TCACCTGGAT GAGTTGACAA GAGCCTATCA GTTTGATATA
    GCAAAACAAA CATGGCACAT TCAAAGCAAT AATCAACCTC CACAGCCTCC AGTTCCACCG
    TTAGGTTATG TGTCTGGAGC CTTGATTTCT GATTTGGAAA CGGATGTTGC AGATGATGAT
    GCCGACGACG AAGAGGAAGC TTTAGAAATC CCCAGGCCCC TGAGAGCACT GGACCAGACT
    CCTGGATCCA GCATGGACAA TCTAGACAGC TCTGTGACAG GAAAAGCCTT TACCTCCTCT
    CAAAGACCTC GACCTACCAG CCCATTTTCT ACTGACAGTA ACACCAGTGC AGCCCTGAGT
    CAAAGTCAGA GGCCTCGGCC CACTAAAAAA CACAAGGGAG GGCGGATGGA CCAACAACCA
    GCATTGCCTC ATCGAAGGGA AGGAATGACA GATGAGGAGG CCTTGGTGCC CTATAGCAAG
    CCCAGTTTCC CATCTCCAGG TGGCCACAGC TCATCAGGAA CAGCTTCTTC TAAGGGATCC
    ACTGGACCTA GGAAAACCGA GGTGTTGAGA GCAGGCCACC AGCGCAATGC CAGCGACCTT
    CTTGACATAG GATATATGGG CTCCAACAGT CAAGGACAGT TTACAGGTGA ATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002933.1
    CDS644..4780
    Translation

    Target ORF information:

    RefSeq Version NM_002942.4
    Organism Homo sapiens(human)
    Definition Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_002942.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC
    ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC
    ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC
    CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG
    AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA
    GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG
    TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA
    GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG
    GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT
    AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA
    AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCCCC CACAGTTTGT GGTTCGGCCA
    AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC
    CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA
    CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC
    ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT
    TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT
    CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA
    GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT
    AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT
    TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT
    GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC
    CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG
    TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC
    AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT
    ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC
    CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA
    CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT
    CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC
    CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG
    ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC
    AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA
    GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA
    CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG
    GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA
    GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA
    ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA
    GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC
    ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC
    TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG
    TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA
    GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC
    TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT
    GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA
    GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT
    ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA
    CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG
    AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG
    AACAAAGGTA ACAATGGTGG GAAAGGTGGA AAAAAGAAGA AAAATAAAAA CTCTTCTAAA
    CCACAGAAAA ACAATGGATC CACTTGGGCC AATGTCCCTC TACCTCCCCC CCCAGTCCAG
    CCCCTTCCTG GCACGGAGCT GGAACACTAT GCAGTGGAAC AACAAGAAAA TGGCTATGAC
    AGTGATAGCT GGTGCCCACC ATTGCCAGTA CAAACTTACT TACACCAAGG TCTGGAAGAT
    GAACTGGAAG AAGATGATGA TAGGGTCCCA ACACCTCCTG TTCGAGGCGT GGCTTCTTCT
    CCTGCTATCT CCTTTGGACA GCAGTCCACT GCAACTCTTA CTCCATCCCC ACGGGAAGAG
    ATGCAACCCA TGCTGCAGGC TCACCTGGAT GAGTTGACAA GAGCCTATCA GTTTGATATA
    GCAAAACAAA CATGGCACAT TCAAAGCAAT AATCAACCTC CACAGCCTCC AGTTCCACCG
    TTAGGTTATG TGTCTGGAGC CTTGATTTCT GATTTGGAAA CGGATGTTGC AGATGATGAT
    GCCGACGACG AAGAGGAAGC TTTAGAAATC CCCAGGCCCC TGAGAGCACT GGACCAGACT
    CCTGGATCCA GCATGGACAA TCTAGACAGC TCTGTGACAG GAAAAGCCTT TACCTCCTCT
    CAAAGACCTC GACCTACCAG CCCATTTTCT ACTGACAGTA ACACCAGTGC AGCCCTGAGT
    CAAAGTCAGA GGCCTCGGCC CACTAAAAAA CACAAGGGAG GGCGGATGGA CCAACAACCA
    GCATTGCCTC ATCGAAGGGA AGGAATGACA GATGAGGAGG CCTTGGTGCC CTATAGCAAG
    CCCAGTTTCC CATCTCCAGG TGGCCACAGC TCATCAGGAA CAGCTTCTTC TAAGGGATCC
    ACTGGACCTA GGAAAACCGA GGTGTTGAGA GCAGGCCACC AGCGCAATGC CAGCGACCTT
    CTTGACATAG GATATATGGG CTCCAACAGT CAAGGACAGT TTACAGGTGA ATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu11403
    Clone ID Related Accession (Same CDS sequence) NM_002942.4 , NM_002942.5
    Accession Version NM_002942.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4137bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product roundabout homolog 2 isoform ROBO2b precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC117515.8, DQ533874.1 and AC133040.3. On Jun 2, 2019 this sequence version replaced NM_002942.4. Transcript Variant: This variant (2) lacks two alternate in-frame exons in the coding region compared to variant 4. The encoded protein (isoform ROBO2b; PMID 16829019) is shorter than variant 4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ533874.1, BC146772.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154665, SAMEA2157437 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC
    ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC
    ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC
    CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG
    AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA
    GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG
    TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA
    GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG
    GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT
    AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA
    AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCCCC CACAGTTTGT GGTTCGGCCA
    AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC
    CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA
    CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC
    ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT
    TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT
    CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA
    GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT
    AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT
    TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT
    GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC
    CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG
    TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC
    AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT
    ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC
    CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA
    CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT
    CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC
    CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG
    ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC
    AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA
    GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA
    CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG
    GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA
    GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA
    ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA
    GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC
    ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC
    TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG
    TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA
    GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC
    TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT
    GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA
    GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT
    ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA
    CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG
    AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG
    AACAAAGGTA ACAATGGTGG GAAAGGTGGA AAAAAGAAGA AAAATAAAAA CTCTTCTAAA
    CCACAGAAAA ACAATGGATC CACTTGGGCC AATGTCCCTC TACCTCCCCC CCCAGTCCAG
    CCCCTTCCTG GCACGGAGCT GGAACACTAT GCAGTGGAAC AACAAGAAAA TGGCTATGAC
    AGTGATAGCT GGTGCCCACC ATTGCCAGTA CAAACTTACT TACACCAAGG TCTGGAAGAT
    GAACTGGAAG AAGATGATGA TAGGGTCCCA ACACCTCCTG TTCGAGGCGT GGCTTCTTCT
    CCTGCTATCT CCTTTGGACA GCAGTCCACT GCAACTCTTA CTCCATCCCC ACGGGAAGAG
    ATGCAACCCA TGCTGCAGGC TCACCTGGAT GAGTTGACAA GAGCCTATCA GTTTGATATA
    GCAAAACAAA CATGGCACAT TCAAAGCAAT AATCAACCTC CACAGCCTCC AGTTCCACCG
    TTAGGTTATG TGTCTGGAGC CTTGATTTCT GATTTGGAAA CGGATGTTGC AGATGATGAT
    GCCGACGACG AAGAGGAAGC TTTAGAAATC CCCAGGCCCC TGAGAGCACT GGACCAGACT
    CCTGGATCCA GCATGGACAA TCTAGACAGC TCTGTGACAG GAAAAGCCTT TACCTCCTCT
    CAAAGACCTC GACCTACCAG CCCATTTTCT ACTGACAGTA ACACCAGTGC AGCCCTGAGT
    CAAAGTCAGA GGCCTCGGCC CACTAAAAAA CACAAGGGAG GGCGGATGGA CCAACAACCA
    GCATTGCCTC ATCGAAGGGA AGGAATGACA GATGAGGAGG CCTTGGTGCC CTATAGCAAG
    CCCAGTTTCC CATCTCCAGG TGGCCACAGC TCATCAGGAA CAGCTTCTTC TAAGGGATCC
    ACTGGACCTA GGAAAACCGA GGTGTTGAGA GCAGGCCACC AGCGCAATGC CAGCGACCTT
    CTTGACATAG GATATATGGG CTCCAACAGT CAAGGACAGT TTACAGGTGA ATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002933.1
    CDS688..4824
    Translation

    Target ORF information:

    RefSeq Version NM_002942.5
    Organism Homo sapiens(human)
    Definition Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_002942.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC
    ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC
    ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC
    CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG
    AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA
    GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG
    TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA
    GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG
    GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT
    AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA
    AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCCCC CACAGTTTGT GGTTCGGCCA
    AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC
    CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA
    CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC
    ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT
    TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT
    CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA
    GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT
    AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT
    TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT
    GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC
    CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG
    TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC
    AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT
    ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC
    CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA
    CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT
    CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC
    CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG
    ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC
    AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA
    GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA
    CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG
    GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA
    GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA
    ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA
    GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC
    ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC
    TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG
    TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA
    GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC
    TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT
    GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA
    GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT
    ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA
    CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG
    AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG
    AACAAAGGTA ACAATGGTGG GAAAGGTGGA AAAAAGAAGA AAAATAAAAA CTCTTCTAAA
    CCACAGAAAA ACAATGGATC CACTTGGGCC AATGTCCCTC TACCTCCCCC CCCAGTCCAG
    CCCCTTCCTG GCACGGAGCT GGAACACTAT GCAGTGGAAC AACAAGAAAA TGGCTATGAC
    AGTGATAGCT GGTGCCCACC ATTGCCAGTA CAAACTTACT TACACCAAGG TCTGGAAGAT
    GAACTGGAAG AAGATGATGA TAGGGTCCCA ACACCTCCTG TTCGAGGCGT GGCTTCTTCT
    CCTGCTATCT CCTTTGGACA GCAGTCCACT GCAACTCTTA CTCCATCCCC ACGGGAAGAG
    ATGCAACCCA TGCTGCAGGC TCACCTGGAT GAGTTGACAA GAGCCTATCA GTTTGATATA
    GCAAAACAAA CATGGCACAT TCAAAGCAAT AATCAACCTC CACAGCCTCC AGTTCCACCG
    TTAGGTTATG TGTCTGGAGC CTTGATTTCT GATTTGGAAA CGGATGTTGC AGATGATGAT
    GCCGACGACG AAGAGGAAGC TTTAGAAATC CCCAGGCCCC TGAGAGCACT GGACCAGACT
    CCTGGATCCA GCATGGACAA TCTAGACAGC TCTGTGACAG GAAAAGCCTT TACCTCCTCT
    CAAAGACCTC GACCTACCAG CCCATTTTCT ACTGACAGTA ACACCAGTGC AGCCCTGAGT
    CAAAGTCAGA GGCCTCGGCC CACTAAAAAA CACAAGGGAG GGCGGATGGA CCAACAACCA
    GCATTGCCTC ATCGAAGGGA AGGAATGACA GATGAGGAGG CCTTGGTGCC CTATAGCAAG
    CCCAGTTTCC CATCTCCAGG TGGCCACAGC TCATCAGGAA CAGCTTCTTC TAAGGGATCC
    ACTGGACCTA GGAAAACCGA GGTGTTGAGA GCAGGCCACC AGCGCAATGC CAGCGACCTT
    CTTGACATAG GATATATGGG CTCCAACAGT CAAGGACAGT TTACAGGTGA ATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu12785
    Clone ID Related Accession (Same CDS sequence) NM_001290040.1 , NM_001290040.2
    Accession Version NM_001290040.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4332bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-29
    Organism Homo sapiens(human)
    Product roundabout homolog 2 isoform 4 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ533874.1, BC144303.1 and AC133040.3. On Feb 22, 2014 this sequence version replaced XM_005264768.1. Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (4). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC144303.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142363, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC
    ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC
    ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC
    CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG
    AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA
    GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG
    TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA
    GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG
    GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT
    AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA
    AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCGCC CTGTTGCTCC CCCACAGTTT
    GTGGTTCGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT
    TTCCCAAACC AACCCCAGCA GCCCAACAGT AGATGCTCAG TGTCACCAAC TGGAGACCTC
    ACAATCACCA ACATTCAACG TTCCGACGCG GGTTACTACA TCTGCCAGGC TTTAACTGTG
    GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAGGTTACTG ATGTTTTGAC AGATAGACCT
    CCACCTATAA TTCTACAAGG CCCAGCCAAC CAAACGCTGG CAGTGGATGG TACAGCGTTA
    CTGAAATGTA AAGCCACTGG TGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT
    ACTTTTCCGG GTAGAGATCC AAGAGCAACA ATTCAAGAGC AAGGCACACT GCAGATTAAG
    AATTTACGGA TTTCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGAGAG
    ACTTCCTGGA GTGCAGTGCT GGATGTGACA GAGTCTGGAG CAACAATCAG TAAAAACTAT
    GATTTAAGTG ACCTGCCAGG GCCACCATCC AAACCGCAGG TCACTGATGT TACTAAGAAC
    AGTGTCACCT TGTCCTGGCA GCCAGGTACC CCTGGAACCC TTCCAGCAAG TGCATATATC
    ATTGAGGCTT TCAGCCAATC AGTGAGCAAC AGCTGGCAGA CCGTGGCAAA CCATGTAAAG
    ACCACCCTCT ATACTGTAAG AGGACTGCGG CCCAATACAA TCTACTTATT CATGGTCAGA
    GCGATCAACC CCCAAGGTCT CAGTGACCCA AGTCCCATGT CAGATCCTGT GCGCACACAA
    GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TGCAGAAAGA GCTAGGAGAT
    GTCCTTGTCC GTCTTCATAA TCCAGTTGTG CTGACTCCCA CCACGGTTCA GGTCACATGG
    ACGGTTGATC GCCAACCCCA GTTTATCCAA GGCTACCGAG TGATGTATCG TCAGACTTCA
    GGTCTGCAGG CGACATCTTC GTGGCAGAAT TTAGATGCCA AAGTCCCGAC TGAACGAAGT
    GCTGTCTTAG TCAACCTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTACG GCCATATTTT
    AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACGGTTC GTACTACTGA AGAAGCCCCA
    AGTGCCCCAC CACAGTCTGT CACTGTACTG ACAGTTGGAA GCTACAATAG CACAAGTATT
    AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CACCAGAATG GAATTATCCA AGAATACAAG
    ATCTGGTGTC TAGGAAATGA AACGCGATTC CATATCAACA AAACTGTGGA TGCAGCCATT
    CGGTCCGTAA TAATTGGTGG ATTATTCCCA GGTATTCAAT ACCGGGTAGA GGTTGCAGCT
    AGTACCAGTG CAGGGGTTGG AGTAAAGAGT GAGCCACAGC CAATAATAAT CGGGAGACGC
    AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTGGTG
    AAGCAACCAG CCTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT
    AGCATATGGT TGTATTGGCG AAGAAAGAAG AGGAAGGGAC TCAGTAATTA TGCTGTTACG
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GTCCAGGTCT TCTCAATGCT
    GGTGATCCCA GCTATCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGCTT GCCAGTAAAT
    AATAGCAACA GTGGCCCAAA TGAGATTGGA AATTTTGGCC GTGGAGATGT GCTGCCACCA
    GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGTAGC
    ATTGACTTCA CTACCAAAAC CAGTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA
    TATGCCACGA CACAGATCTT GCATTCCAAC AGCATACATG AATTGGCTGT CGATCTGCCT
    GATCCACAAT GGAAAAGCTC AATTCAGCAA AAAACAGATC TGATGGGATT TGGTTATTCT
    CTACCTGATC AGAACAAAGG TAACAATGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA
    AACTCTTCTA AACCACAGAA AAACAATGGA TCCACTTGGG CCAATGTCCC TCTACCTCCC
    CCCCCAGTCC AGCCCCTTCC TGGCACGGAG CTGGAACACT ATGCAGTGGA ACAACAAGAA
    AATGGCTATG ACAGTGATAG CTGGTGCCCA CCATTGCCAG TACAAACTTA CTTACACCAA
    GGTCTGGAAG ATGAACTGGA AGAAGATGAT GATAGGGTCC CAACACCTCC TGTTCGAGGC
    GTGGCTTCTT CTCCTGCTAT CTCCTTTGGA CAGCAGTCCA CTGCAACTCT TACTCCATCC
    CCACGGGAAG AGATGCAACC CATGCTGCAG GCTCACCTGG ATGAGTTGAC AAGAGCCTAT
    CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATAATCAACC TCCACAGCCT
    CCAGTTCCAC CGTTAGGTTA TGTGTCTGGA GCCTTGATTT CTGATTTGGA AACGGATGTT
    GCAGATGATG ATGCCGACGA CGAAGAGGAA GCTTTAGAAA TCCCCAGGCC CCTGAGAGCA
    CTGGACCAGA CTCCTGGATC CAGCATGGAC AATCTAGACA GCTCTGTGAC AGGTTCAATG
    GTAAATGGAT GGGGCTCTGC ATCTGATGAG GATCGTAACT TTTCTAGTCA TAGATCTAGT
    GTAGGTAGCT CCTCAGATGG CTCTATCTTT GCCAGCGGCA GTTTTGCACA AGCACTGGTG
    GCAGCAGCAG ATAAAGCTGG TTTTAGGCTG GATGGAACCA GCCTTACAAG AACAGGAAAA
    GCCTTTACCT CCTCTCAAAG ACCTCGACCT ACCAGCCCAT TTTCTACTGA CAGTAACACC
    AGTGCAGCCC TGAGTCAAAG TCAGAGGCCT CGGCCCACTA AAAAACACAA GGGAGGGCGG
    ATGGACCAAC AACCAGCATT GCCTCATCGA AGGGAAGGAA TGACAGATGA GGAGGCCTTG
    GTGCCCTATA GCAAGCCCAG TTTCCCATCT CCAGGTGGCC ACAGCTCATC AGGAACAGCT
    TCTTCTAAGG GATCCACTGG ACCTAGGAAA ACCGAGGTGT TGAGAGCAGG CCACCAGCGC
    AATGCCAGCG ACCTTCTTGA CATAGGATAT ATGGGCTCCA ACAGTCAAGG ACAGTTTACA
    GGTGAATTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001276969.1
    CDS644..4975
    Translation

    Target ORF information:

    RefSeq Version NM_001290040.1
    Organism Homo sapiens(human)
    Definition Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001290040.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC
    ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC
    ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC
    CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG
    AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA
    GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG
    TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA
    GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG
    GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT
    AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA
    AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCGCC CTGTTGCTCC CCCACAGTTT
    GTGGTTCGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT
    TTCCCAAACC AACCCCAGCA GCCCAACAGT AGATGCTCAG TGTCACCAAC TGGAGACCTC
    ACAATCACCA ACATTCAACG TTCCGACGCG GGTTACTACA TCTGCCAGGC TTTAACTGTG
    GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAGGTTACTG ATGTTTTGAC AGATAGACCT
    CCACCTATAA TTCTACAAGG CCCAGCCAAC CAAACGCTGG CAGTGGATGG TACAGCGTTA
    CTGAAATGTA AAGCCACTGG TGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT
    ACTTTTCCGG GTAGAGATCC AAGAGCAACA ATTCAAGAGC AAGGCACACT GCAGATTAAG
    AATTTACGGA TTTCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGAGAG
    ACTTCCTGGA GTGCAGTGCT GGATGTGACA GAGTCTGGAG CAACAATCAG TAAAAACTAT
    GATTTAAGTG ACCTGCCAGG GCCACCATCC AAACCGCAGG TCACTGATGT TACTAAGAAC
    AGTGTCACCT TGTCCTGGCA GCCAGGTACC CCTGGAACCC TTCCAGCAAG TGCATATATC
    ATTGAGGCTT TCAGCCAATC AGTGAGCAAC AGCTGGCAGA CCGTGGCAAA CCATGTAAAG
    ACCACCCTCT ATACTGTAAG AGGACTGCGG CCCAATACAA TCTACTTATT CATGGTCAGA
    GCGATCAACC CCCAAGGTCT CAGTGACCCA AGTCCCATGT CAGATCCTGT GCGCACACAA
    GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TGCAGAAAGA GCTAGGAGAT
    GTCCTTGTCC GTCTTCATAA TCCAGTTGTG CTGACTCCCA CCACGGTTCA GGTCACATGG
    ACGGTTGATC GCCAACCCCA GTTTATCCAA GGCTACCGAG TGATGTATCG TCAGACTTCA
    GGTCTGCAGG CGACATCTTC GTGGCAGAAT TTAGATGCCA AAGTCCCGAC TGAACGAAGT
    GCTGTCTTAG TCAACCTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTACG GCCATATTTT
    AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACGGTTC GTACTACTGA AGAAGCCCCA
    AGTGCCCCAC CACAGTCTGT CACTGTACTG ACAGTTGGAA GCTACAATAG CACAAGTATT
    AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CACCAGAATG GAATTATCCA AGAATACAAG
    ATCTGGTGTC TAGGAAATGA AACGCGATTC CATATCAACA AAACTGTGGA TGCAGCCATT
    CGGTCCGTAA TAATTGGTGG ATTATTCCCA GGTATTCAAT ACCGGGTAGA GGTTGCAGCT
    AGTACCAGTG CAGGGGTTGG AGTAAAGAGT GAGCCACAGC CAATAATAAT CGGGAGACGC
    AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTGGTG
    AAGCAACCAG CCTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT
    AGCATATGGT TGTATTGGCG AAGAAAGAAG AGGAAGGGAC TCAGTAATTA TGCTGTTACG
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GTCCAGGTCT TCTCAATGCT
    GGTGATCCCA GCTATCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGCTT GCCAGTAAAT
    AATAGCAACA GTGGCCCAAA TGAGATTGGA AATTTTGGCC GTGGAGATGT GCTGCCACCA
    GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGTAGC
    ATTGACTTCA CTACCAAAAC CAGTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA
    TATGCCACGA CACAGATCTT GCATTCCAAC AGCATACATG AATTGGCTGT CGATCTGCCT
    GATCCACAAT GGAAAAGCTC AATTCAGCAA AAAACAGATC TGATGGGATT TGGTTATTCT
    CTACCTGATC AGAACAAAGG TAACAATGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA
    AACTCTTCTA AACCACAGAA AAACAATGGA TCCACTTGGG CCAATGTCCC TCTACCTCCC
    CCCCCAGTCC AGCCCCTTCC TGGCACGGAG CTGGAACACT ATGCAGTGGA ACAACAAGAA
    AATGGCTATG ACAGTGATAG CTGGTGCCCA CCATTGCCAG TACAAACTTA CTTACACCAA
    GGTCTGGAAG ATGAACTGGA AGAAGATGAT GATAGGGTCC CAACACCTCC TGTTCGAGGC
    GTGGCTTCTT CTCCTGCTAT CTCCTTTGGA CAGCAGTCCA CTGCAACTCT TACTCCATCC
    CCACGGGAAG AGATGCAACC CATGCTGCAG GCTCACCTGG ATGAGTTGAC AAGAGCCTAT
    CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATAATCAACC TCCACAGCCT
    CCAGTTCCAC CGTTAGGTTA TGTGTCTGGA GCCTTGATTT CTGATTTGGA AACGGATGTT
    GCAGATGATG ATGCCGACGA CGAAGAGGAA GCTTTAGAAA TCCCCAGGCC CCTGAGAGCA
    CTGGACCAGA CTCCTGGATC CAGCATGGAC AATCTAGACA GCTCTGTGAC AGGTTCAATG
    GTAAATGGAT GGGGCTCTGC ATCTGATGAG GATCGTAACT TTTCTAGTCA TAGATCTAGT
    GTAGGTAGCT CCTCAGATGG CTCTATCTTT GCCAGCGGCA GTTTTGCACA AGCACTGGTG
    GCAGCAGCAG ATAAAGCTGG TTTTAGGCTG GATGGAACCA GCCTTACAAG AACAGGAAAA
    GCCTTTACCT CCTCTCAAAG ACCTCGACCT ACCAGCCCAT TTTCTACTGA CAGTAACACC
    AGTGCAGCCC TGAGTCAAAG TCAGAGGCCT CGGCCCACTA AAAAACACAA GGGAGGGCGG
    ATGGACCAAC AACCAGCATT GCCTCATCGA AGGGAAGGAA TGACAGATGA GGAGGCCTTG
    GTGCCCTATA GCAAGCCCAG TTTCCCATCT CCAGGTGGCC ACAGCTCATC AGGAACAGCT
    TCTTCTAAGG GATCCACTGG ACCTAGGAAA ACCGAGGTGT TGAGAGCAGG CCACCAGCGC
    AATGCCAGCG ACCTTCTTGA CATAGGATAT ATGGGCTCCA ACAGTCAAGG ACAGTTTACA
    GGTGAATTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu12785
    Clone ID Related Accession (Same CDS sequence) NM_001290040.1 , NM_001290040.2
    Accession Version NM_001290040.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4332bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product roundabout homolog 2 isoform 4 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC117515.8, DQ533874.1, BC144303.1 and AC133040.3. On Jun 2, 2019 this sequence version replaced NM_001290040.1. Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (4). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC144303.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142363, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC
    ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC
    ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC
    CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG
    AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA
    GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG
    TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA
    GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG
    GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT
    AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA
    AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCGCC CTGTTGCTCC CCCACAGTTT
    GTGGTTCGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT
    TTCCCAAACC AACCCCAGCA GCCCAACAGT AGATGCTCAG TGTCACCAAC TGGAGACCTC
    ACAATCACCA ACATTCAACG TTCCGACGCG GGTTACTACA TCTGCCAGGC TTTAACTGTG
    GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAGGTTACTG ATGTTTTGAC AGATAGACCT
    CCACCTATAA TTCTACAAGG CCCAGCCAAC CAAACGCTGG CAGTGGATGG TACAGCGTTA
    CTGAAATGTA AAGCCACTGG TGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT
    ACTTTTCCGG GTAGAGATCC AAGAGCAACA ATTCAAGAGC AAGGCACACT GCAGATTAAG
    AATTTACGGA TTTCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGAGAG
    ACTTCCTGGA GTGCAGTGCT GGATGTGACA GAGTCTGGAG CAACAATCAG TAAAAACTAT
    GATTTAAGTG ACCTGCCAGG GCCACCATCC AAACCGCAGG TCACTGATGT TACTAAGAAC
    AGTGTCACCT TGTCCTGGCA GCCAGGTACC CCTGGAACCC TTCCAGCAAG TGCATATATC
    ATTGAGGCTT TCAGCCAATC AGTGAGCAAC AGCTGGCAGA CCGTGGCAAA CCATGTAAAG
    ACCACCCTCT ATACTGTAAG AGGACTGCGG CCCAATACAA TCTACTTATT CATGGTCAGA
    GCGATCAACC CCCAAGGTCT CAGTGACCCA AGTCCCATGT CAGATCCTGT GCGCACACAA
    GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TGCAGAAAGA GCTAGGAGAT
    GTCCTTGTCC GTCTTCATAA TCCAGTTGTG CTGACTCCCA CCACGGTTCA GGTCACATGG
    ACGGTTGATC GCCAACCCCA GTTTATCCAA GGCTACCGAG TGATGTATCG TCAGACTTCA
    GGTCTGCAGG CGACATCTTC GTGGCAGAAT TTAGATGCCA AAGTCCCGAC TGAACGAAGT
    GCTGTCTTAG TCAACCTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTACG GCCATATTTT
    AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACGGTTC GTACTACTGA AGAAGCCCCA
    AGTGCCCCAC CACAGTCTGT CACTGTACTG ACAGTTGGAA GCTACAATAG CACAAGTATT
    AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CACCAGAATG GAATTATCCA AGAATACAAG
    ATCTGGTGTC TAGGAAATGA AACGCGATTC CATATCAACA AAACTGTGGA TGCAGCCATT
    CGGTCCGTAA TAATTGGTGG ATTATTCCCA GGTATTCAAT ACCGGGTAGA GGTTGCAGCT
    AGTACCAGTG CAGGGGTTGG AGTAAAGAGT GAGCCACAGC CAATAATAAT CGGGAGACGC
    AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTGGTG
    AAGCAACCAG CCTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT
    AGCATATGGT TGTATTGGCG AAGAAAGAAG AGGAAGGGAC TCAGTAATTA TGCTGTTACG
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GTCCAGGTCT TCTCAATGCT
    GGTGATCCCA GCTATCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGCTT GCCAGTAAAT
    AATAGCAACA GTGGCCCAAA TGAGATTGGA AATTTTGGCC GTGGAGATGT GCTGCCACCA
    GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGTAGC
    ATTGACTTCA CTACCAAAAC CAGTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA
    TATGCCACGA CACAGATCTT GCATTCCAAC AGCATACATG AATTGGCTGT CGATCTGCCT
    GATCCACAAT GGAAAAGCTC AATTCAGCAA AAAACAGATC TGATGGGATT TGGTTATTCT
    CTACCTGATC AGAACAAAGG TAACAATGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA
    AACTCTTCTA AACCACAGAA AAACAATGGA TCCACTTGGG CCAATGTCCC TCTACCTCCC
    CCCCCAGTCC AGCCCCTTCC TGGCACGGAG CTGGAACACT ATGCAGTGGA ACAACAAGAA
    AATGGCTATG ACAGTGATAG CTGGTGCCCA CCATTGCCAG TACAAACTTA CTTACACCAA
    GGTCTGGAAG ATGAACTGGA AGAAGATGAT GATAGGGTCC CAACACCTCC TGTTCGAGGC
    GTGGCTTCTT CTCCTGCTAT CTCCTTTGGA CAGCAGTCCA CTGCAACTCT TACTCCATCC
    CCACGGGAAG AGATGCAACC CATGCTGCAG GCTCACCTGG ATGAGTTGAC AAGAGCCTAT
    CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATAATCAACC TCCACAGCCT
    CCAGTTCCAC CGTTAGGTTA TGTGTCTGGA GCCTTGATTT CTGATTTGGA AACGGATGTT
    GCAGATGATG ATGCCGACGA CGAAGAGGAA GCTTTAGAAA TCCCCAGGCC CCTGAGAGCA
    CTGGACCAGA CTCCTGGATC CAGCATGGAC AATCTAGACA GCTCTGTGAC AGGTTCAATG
    GTAAATGGAT GGGGCTCTGC ATCTGATGAG GATCGTAACT TTTCTAGTCA TAGATCTAGT
    GTAGGTAGCT CCTCAGATGG CTCTATCTTT GCCAGCGGCA GTTTTGCACA AGCACTGGTG
    GCAGCAGCAG ATAAAGCTGG TTTTAGGCTG GATGGAACCA GCCTTACAAG AACAGGAAAA
    GCCTTTACCT CCTCTCAAAG ACCTCGACCT ACCAGCCCAT TTTCTACTGA CAGTAACACC
    AGTGCAGCCC TGAGTCAAAG TCAGAGGCCT CGGCCCACTA AAAAACACAA GGGAGGGCGG
    ATGGACCAAC AACCAGCATT GCCTCATCGA AGGGAAGGAA TGACAGATGA GGAGGCCTTG
    GTGCCCTATA GCAAGCCCAG TTTCCCATCT CCAGGTGGCC ACAGCTCATC AGGAACAGCT
    TCTTCTAAGG GATCCACTGG ACCTAGGAAA ACCGAGGTGT TGAGAGCAGG CCACCAGCGC
    AATGCCAGCG ACCTTCTTGA CATAGGATAT ATGGGCTCCA ACAGTCAAGG ACAGTTTACA
    GGTGAATTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001276969.1
    CDS688..5019
    Translation

    Target ORF information:

    RefSeq Version NM_001290040.2
    Organism Homo sapiens(human)
    Definition Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001290040.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC
    ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC
    ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC
    CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG
    AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA
    GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG
    TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA
    GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG
    GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT
    AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA
    AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCGCC CTGTTGCTCC CCCACAGTTT
    GTGGTTCGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT
    TTCCCAAACC AACCCCAGCA GCCCAACAGT AGATGCTCAG TGTCACCAAC TGGAGACCTC
    ACAATCACCA ACATTCAACG TTCCGACGCG GGTTACTACA TCTGCCAGGC TTTAACTGTG
    GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAGGTTACTG ATGTTTTGAC AGATAGACCT
    CCACCTATAA TTCTACAAGG CCCAGCCAAC CAAACGCTGG CAGTGGATGG TACAGCGTTA
    CTGAAATGTA AAGCCACTGG TGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT
    ACTTTTCCGG GTAGAGATCC AAGAGCAACA ATTCAAGAGC AAGGCACACT GCAGATTAAG
    AATTTACGGA TTTCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGAGAG
    ACTTCCTGGA GTGCAGTGCT GGATGTGACA GAGTCTGGAG CAACAATCAG TAAAAACTAT
    GATTTAAGTG ACCTGCCAGG GCCACCATCC AAACCGCAGG TCACTGATGT TACTAAGAAC
    AGTGTCACCT TGTCCTGGCA GCCAGGTACC CCTGGAACCC TTCCAGCAAG TGCATATATC
    ATTGAGGCTT TCAGCCAATC AGTGAGCAAC AGCTGGCAGA CCGTGGCAAA CCATGTAAAG
    ACCACCCTCT ATACTGTAAG AGGACTGCGG CCCAATACAA TCTACTTATT CATGGTCAGA
    GCGATCAACC CCCAAGGTCT CAGTGACCCA AGTCCCATGT CAGATCCTGT GCGCACACAA
    GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TGCAGAAAGA GCTAGGAGAT
    GTCCTTGTCC GTCTTCATAA TCCAGTTGTG CTGACTCCCA CCACGGTTCA GGTCACATGG
    ACGGTTGATC GCCAACCCCA GTTTATCCAA GGCTACCGAG TGATGTATCG TCAGACTTCA
    GGTCTGCAGG CGACATCTTC GTGGCAGAAT TTAGATGCCA AAGTCCCGAC TGAACGAAGT
    GCTGTCTTAG TCAACCTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTACG GCCATATTTT
    AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACGGTTC GTACTACTGA AGAAGCCCCA
    AGTGCCCCAC CACAGTCTGT CACTGTACTG ACAGTTGGAA GCTACAATAG CACAAGTATT
    AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CACCAGAATG GAATTATCCA AGAATACAAG
    ATCTGGTGTC TAGGAAATGA AACGCGATTC CATATCAACA AAACTGTGGA TGCAGCCATT
    CGGTCCGTAA TAATTGGTGG ATTATTCCCA GGTATTCAAT ACCGGGTAGA GGTTGCAGCT
    AGTACCAGTG CAGGGGTTGG AGTAAAGAGT GAGCCACAGC CAATAATAAT CGGGAGACGC
    AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTGGTG
    AAGCAACCAG CCTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT
    AGCATATGGT TGTATTGGCG AAGAAAGAAG AGGAAGGGAC TCAGTAATTA TGCTGTTACG
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GTCCAGGTCT TCTCAATGCT
    GGTGATCCCA GCTATCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGCTT GCCAGTAAAT
    AATAGCAACA GTGGCCCAAA TGAGATTGGA AATTTTGGCC GTGGAGATGT GCTGCCACCA
    GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGTAGC
    ATTGACTTCA CTACCAAAAC CAGTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA
    TATGCCACGA CACAGATCTT GCATTCCAAC AGCATACATG AATTGGCTGT CGATCTGCCT
    GATCCACAAT GGAAAAGCTC AATTCAGCAA AAAACAGATC TGATGGGATT TGGTTATTCT
    CTACCTGATC AGAACAAAGG TAACAATGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA
    AACTCTTCTA AACCACAGAA AAACAATGGA TCCACTTGGG CCAATGTCCC TCTACCTCCC
    CCCCCAGTCC AGCCCCTTCC TGGCACGGAG CTGGAACACT ATGCAGTGGA ACAACAAGAA
    AATGGCTATG ACAGTGATAG CTGGTGCCCA CCATTGCCAG TACAAACTTA CTTACACCAA
    GGTCTGGAAG ATGAACTGGA AGAAGATGAT GATAGGGTCC CAACACCTCC TGTTCGAGGC
    GTGGCTTCTT CTCCTGCTAT CTCCTTTGGA CAGCAGTCCA CTGCAACTCT TACTCCATCC
    CCACGGGAAG AGATGCAACC CATGCTGCAG GCTCACCTGG ATGAGTTGAC AAGAGCCTAT
    CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATAATCAACC TCCACAGCCT
    CCAGTTCCAC CGTTAGGTTA TGTGTCTGGA GCCTTGATTT CTGATTTGGA AACGGATGTT
    GCAGATGATG ATGCCGACGA CGAAGAGGAA GCTTTAGAAA TCCCCAGGCC CCTGAGAGCA
    CTGGACCAGA CTCCTGGATC CAGCATGGAC AATCTAGACA GCTCTGTGAC AGGTTCAATG
    GTAAATGGAT GGGGCTCTGC ATCTGATGAG GATCGTAACT TTTCTAGTCA TAGATCTAGT
    GTAGGTAGCT CCTCAGATGG CTCTATCTTT GCCAGCGGCA GTTTTGCACA AGCACTGGTG
    GCAGCAGCAG ATAAAGCTGG TTTTAGGCTG GATGGAACCA GCCTTACAAG AACAGGAAAA
    GCCTTTACCT CCTCTCAAAG ACCTCGACCT ACCAGCCCAT TTTCTACTGA CAGTAACACC
    AGTGCAGCCC TGAGTCAAAG TCAGAGGCCT CGGCCCACTA AAAAACACAA GGGAGGGCGG
    ATGGACCAAC AACCAGCATT GCCTCATCGA AGGGAAGGAA TGACAGATGA GGAGGCCTTG
    GTGCCCTATA GCAAGCCCAG TTTCCCATCT CCAGGTGGCC ACAGCTCATC AGGAACAGCT
    TCTTCTAAGG GATCCACTGG ACCTAGGAAA ACCGAGGTGT TGAGAGCAGG CCACCAGCGC
    AATGCCAGCG ACCTTCTTGA CATAGGATAT ATGGGCTCCA ACAGTCAAGG ACAGTTTACA
    GGTGAATTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu12905
    Clone ID Related Accession (Same CDS sequence) NM_001290039.2 , NM_001290039.1
    Accession Version NM_001290039.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product roundabout homolog 2 isoform 3 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC117515.8, DQ533874.1, BC144306.1, AC117516.5 and AC133040.3. On Jun 2, 2019 this sequence version replaced NM_001290039.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 3' coding region compared to variant 4. The encoded protein (isoform 3) is shorter than variant 4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.13775.1, SRR1803613.148636.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142363, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC
    ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC
    ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC
    CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG
    AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA
    GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG
    TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA
    GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG
    GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT
    AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA
    AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCGCC CTGTTGCTCC CCCACAGTTT
    GTGGTTCGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT
    TTCCCAAACC AACCCCAGCA GCCCAACAGT AGATGCTCAG TGTCACCAAC TGGAGACCTC
    ACAATCACCA ACATTCAACG TTCCGACGCG GGTTACTACA TCTGCCAGGC TTTAACTGTG
    GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAGGTTACTG ATGTTTTGAC AGATAGACCT
    CCACCTATAA TTCTACAAGG CCCAGCCAAC CAAACGCTGG CAGTGGATGG TACAGCGTTA
    CTGAAATGTA AAGCCACTGG TGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT
    ACTTTTCCGG GTAGAGATCC AAGAGCAACA ATTCAAGAGC AAGGCACACT GCAGATTAAG
    AATTTACGGA TTTCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGAGAG
    ACTTCCTGGA GTGCAGTGCT GGATGTGACA GAGTCTGGAG CAACAATCAG TAAAAACTAT
    GATTTAAGTG ACCTGCCAGG GCCACCATCC AAACCGCAGG TCACTGATGT TACTAAGAAC
    AGTGTCACCT TGTCCTGGCA GCCAGGTACC CCTGGAACCC TTCCAGCAAG TGCATATATC
    ATTGAGGCTT TCAGCCAATC AGTGAGCAAC AGCTGGCAGA CCGTGGCAAA CCATGTAAAG
    ACCACCCTCT ATACTGTAAG AGGACTGCGG CCCAATACAA TCTACTTATT CATGGTCAGA
    GCGATCAACC CCCAAGGTCT CAGTGACCCA AGTCCCATGT CAGATCCTGT GCGCACACAA
    GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TGCAGAAAGA GCTAGGAGAT
    GTCCTTGTCC GTCTTCATAA TCCAGTTGTG CTGACTCCCA CCACGGTTCA GGTCACATGG
    ACGGTTGATC GCCAACCCCA GTTTATCCAA GGCTACCGAG TGATGTATCG TCAGACTTCA
    GGTCTGCAGG CGACATCTTC GTGGCAGAAT TTAGATGCCA AAGTCCCGAC TGAACGAAGT
    GCTGTCTTAG TCAACCTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTACG GCCATATTTT
    AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACGGTTC GTACTACTGA AGAAGCCCCA
    AGTGCCCCAC CACAGTCTGT CACTGTACTG ACAGTTGGAA GCTACAATAG CACAAGTATT
    AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CACCAGAATG GAATTATCCA AGAATACAAG
    ATCTGGTGTC TAGGAAATGA AACGCGATTC CATATCAACA AAACTGTGGA TGCAGCCATT
    CGGTCCGTAA TAATTGGTGG ATTATTCCCA GGTATTCAAT ACCGGGTAGA GGTTGCAGCT
    AGTACCAGTG CAGGGGTTGG AGTAAAGAGT GAGCCACAGC CAATAATAAT CGGGAGACGC
    AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTGGTG
    AAGCAACCAG CCTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT
    AGCATATGGT TGTATTGGCG AAGAAAGAAG AGGAAGGGAC TCAGTAATTA TGCTGTTACG
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GTCCAGGTCT TCTCAATGCT
    GGTGATCCCA GCTATCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGCTT GCCAGTAAAT
    AATAGCAACA GTGGCCCAAA TGAGATTGGA AATTTTGGCC GTGGAGATGT GCTGCCACCA
    GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGTAGC
    ATTGACTTCA CTACCAAAAC CAGTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA
    TATGCCACGA CACAGATCTT GCATTCCAAC AGCATACATG AATTGGCTGT CGATCTGCCT
    GATCCACAAT GGAAAAGCTC AATTCAGCAA AAAACAGATC TGATGGGATT TGGTTATTCT
    CTACCTGATC AGAACAAAGG TAACAATGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA
    AACTCTTCTA AACCACAGAA AAACAATGGA TCCACTTGGG CCAATGTCCC TCTACCTCCC
    CCCCCAGTCC AGCCCCTTCC TGGCACGGAG CTGGAACACT ATGCAGTGGA ACAACAAGAA
    AATGGCTATG ACAGTGATAG CTGGTGCCCA CCATTGCCAG TACAAACTTA CTTACACCAA
    GGTCTGGAAG ATGAACTGGA AGAAGATGAT GATAGGGTCC CAACACCTCC TGTTCGAGGC
    GTGGCTTCTT CTCCTGCTAT CTCCTTTGGA CAGCAGTCCA CTGCAACTCT TACTCCATCC
    CCACGGGAAG AGATGCAACC CATGCTGCAG GCTCACCTGG ATGAGTTGAC AAGAGCCTAT
    CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATAATCAACC TCCACAGCCT
    CCAGTTCCAC CGTTAGGTTA TGTGTCTGGA GCCTTGATTT CTGATTTGGA AACGGATGTT
    GCAGATGATG ATGCCGACGA CGAAGAGGAA GCTTTAGAAA TCCCCAGGCC CCTGAGAGCA
    CTGGACCAGA CTCCTGGATC CAGCATGGAC AATCTAGACA GCTCTGTGAC AGGAAAAGCC
    TTTACCTCCT CTCAAAGACC TCGACCTACC AGCCCATTTT CTACTGACAG TAACACCAGT
    GCAGCCCTGA GTCAAAGTCA GAGGCCTCGG CCCACTAAAA AACACAAGGG AGGGCGGATG
    GACCAACAAC CAGCATTGCC TCATCGAAGG GAAGGAATGA CAGATGAGGA GGCCTTGGTG
    CCCTATAGCA AGCCCAGTTT CCCATCTCCA GGTGGCCACA GCTCATCAGG AACAGCTTCT
    TCTAAGGGAT CCACTGGACC TAGGAAAACC GAGGTGTTGA GAGCAGGCCA CCAGCGCAAT
    GCCAGCGACC TTCTTGACAT AGGATATATG GGCTCCAACA GTCAAGGACA GTTTACAGGT
    GAATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001276968.1
    CDS688..4836
    Translation

    Target ORF information:

    RefSeq Version NM_001290039.2
    Organism Homo sapiens(human)
    Definition Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001290039.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC
    ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC
    ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC
    CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG
    AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA
    GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG
    TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA
    GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG
    GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT
    AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA
    AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCGCC CTGTTGCTCC CCCACAGTTT
    GTGGTTCGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT
    TTCCCAAACC AACCCCAGCA GCCCAACAGT AGATGCTCAG TGTCACCAAC TGGAGACCTC
    ACAATCACCA ACATTCAACG TTCCGACGCG GGTTACTACA TCTGCCAGGC TTTAACTGTG
    GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAGGTTACTG ATGTTTTGAC AGATAGACCT
    CCACCTATAA TTCTACAAGG CCCAGCCAAC CAAACGCTGG CAGTGGATGG TACAGCGTTA
    CTGAAATGTA AAGCCACTGG TGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT
    ACTTTTCCGG GTAGAGATCC AAGAGCAACA ATTCAAGAGC AAGGCACACT GCAGATTAAG
    AATTTACGGA TTTCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGAGAG
    ACTTCCTGGA GTGCAGTGCT GGATGTGACA GAGTCTGGAG CAACAATCAG TAAAAACTAT
    GATTTAAGTG ACCTGCCAGG GCCACCATCC AAACCGCAGG TCACTGATGT TACTAAGAAC
    AGTGTCACCT TGTCCTGGCA GCCAGGTACC CCTGGAACCC TTCCAGCAAG TGCATATATC
    ATTGAGGCTT TCAGCCAATC AGTGAGCAAC AGCTGGCAGA CCGTGGCAAA CCATGTAAAG
    ACCACCCTCT ATACTGTAAG AGGACTGCGG CCCAATACAA TCTACTTATT CATGGTCAGA
    GCGATCAACC CCCAAGGTCT CAGTGACCCA AGTCCCATGT CAGATCCTGT GCGCACACAA
    GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TGCAGAAAGA GCTAGGAGAT
    GTCCTTGTCC GTCTTCATAA TCCAGTTGTG CTGACTCCCA CCACGGTTCA GGTCACATGG
    ACGGTTGATC GCCAACCCCA GTTTATCCAA GGCTACCGAG TGATGTATCG TCAGACTTCA
    GGTCTGCAGG CGACATCTTC GTGGCAGAAT TTAGATGCCA AAGTCCCGAC TGAACGAAGT
    GCTGTCTTAG TCAACCTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTACG GCCATATTTT
    AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACGGTTC GTACTACTGA AGAAGCCCCA
    AGTGCCCCAC CACAGTCTGT CACTGTACTG ACAGTTGGAA GCTACAATAG CACAAGTATT
    AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CACCAGAATG GAATTATCCA AGAATACAAG
    ATCTGGTGTC TAGGAAATGA AACGCGATTC CATATCAACA AAACTGTGGA TGCAGCCATT
    CGGTCCGTAA TAATTGGTGG ATTATTCCCA GGTATTCAAT ACCGGGTAGA GGTTGCAGCT
    AGTACCAGTG CAGGGGTTGG AGTAAAGAGT GAGCCACAGC CAATAATAAT CGGGAGACGC
    AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTGGTG
    AAGCAACCAG CCTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT
    AGCATATGGT TGTATTGGCG AAGAAAGAAG AGGAAGGGAC TCAGTAATTA TGCTGTTACG
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GTCCAGGTCT TCTCAATGCT
    GGTGATCCCA GCTATCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGCTT GCCAGTAAAT
    AATAGCAACA GTGGCCCAAA TGAGATTGGA AATTTTGGCC GTGGAGATGT GCTGCCACCA
    GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGTAGC
    ATTGACTTCA CTACCAAAAC CAGTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA
    TATGCCACGA CACAGATCTT GCATTCCAAC AGCATACATG AATTGGCTGT CGATCTGCCT
    GATCCACAAT GGAAAAGCTC AATTCAGCAA AAAACAGATC TGATGGGATT TGGTTATTCT
    CTACCTGATC AGAACAAAGG TAACAATGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA
    AACTCTTCTA AACCACAGAA AAACAATGGA TCCACTTGGG CCAATGTCCC TCTACCTCCC
    CCCCCAGTCC AGCCCCTTCC TGGCACGGAG CTGGAACACT ATGCAGTGGA ACAACAAGAA
    AATGGCTATG ACAGTGATAG CTGGTGCCCA CCATTGCCAG TACAAACTTA CTTACACCAA
    GGTCTGGAAG ATGAACTGGA AGAAGATGAT GATAGGGTCC CAACACCTCC TGTTCGAGGC
    GTGGCTTCTT CTCCTGCTAT CTCCTTTGGA CAGCAGTCCA CTGCAACTCT TACTCCATCC
    CCACGGGAAG AGATGCAACC CATGCTGCAG GCTCACCTGG ATGAGTTGAC AAGAGCCTAT
    CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATAATCAACC TCCACAGCCT
    CCAGTTCCAC CGTTAGGTTA TGTGTCTGGA GCCTTGATTT CTGATTTGGA AACGGATGTT
    GCAGATGATG ATGCCGACGA CGAAGAGGAA GCTTTAGAAA TCCCCAGGCC CCTGAGAGCA
    CTGGACCAGA CTCCTGGATC CAGCATGGAC AATCTAGACA GCTCTGTGAC AGGAAAAGCC
    TTTACCTCCT CTCAAAGACC TCGACCTACC AGCCCATTTT CTACTGACAG TAACACCAGT
    GCAGCCCTGA GTCAAAGTCA GAGGCCTCGG CCCACTAAAA AACACAAGGG AGGGCGGATG
    GACCAACAAC CAGCATTGCC TCATCGAAGG GAAGGAATGA CAGATGAGGA GGCCTTGGTG
    CCCTATAGCA AGCCCAGTTT CCCATCTCCA GGTGGCCACA GCTCATCAGG AACAGCTTCT
    TCTAAGGGAT CCACTGGACC TAGGAAAACC GAGGTGTTGA GAGCAGGCCA CCAGCGCAAT
    GCCAGCGACC TTCTTGACAT AGGATATATG GGCTCCAACA GTCAAGGACA GTTTACAGGT
    GAATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu12905
    Clone ID Related Accession (Same CDS sequence) NM_001290039.2 , NM_001290039.1
    Accession Version NM_001290039.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product roundabout homolog 2 isoform 3 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ533874.1, BC144306.1, AC117516.5 and AC133040.3. On Feb 22, 2014 this sequence version replaced XM_005264769.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 3' coding region compared to variant 4. The encoded protein (isoform 3) is shorter than variant 4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.13775.1, SRR1803613.148636.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142363, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC
    ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC
    ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC
    CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG
    AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA
    GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG
    TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA
    GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG
    GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT
    AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA
    AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCGCC CTGTTGCTCC CCCACAGTTT
    GTGGTTCGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT
    TTCCCAAACC AACCCCAGCA GCCCAACAGT AGATGCTCAG TGTCACCAAC TGGAGACCTC
    ACAATCACCA ACATTCAACG TTCCGACGCG GGTTACTACA TCTGCCAGGC TTTAACTGTG
    GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAGGTTACTG ATGTTTTGAC AGATAGACCT
    CCACCTATAA TTCTACAAGG CCCAGCCAAC CAAACGCTGG CAGTGGATGG TACAGCGTTA
    CTGAAATGTA AAGCCACTGG TGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT
    ACTTTTCCGG GTAGAGATCC AAGAGCAACA ATTCAAGAGC AAGGCACACT GCAGATTAAG
    AATTTACGGA TTTCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGAGAG
    ACTTCCTGGA GTGCAGTGCT GGATGTGACA GAGTCTGGAG CAACAATCAG TAAAAACTAT
    GATTTAAGTG ACCTGCCAGG GCCACCATCC AAACCGCAGG TCACTGATGT TACTAAGAAC
    AGTGTCACCT TGTCCTGGCA GCCAGGTACC CCTGGAACCC TTCCAGCAAG TGCATATATC
    ATTGAGGCTT TCAGCCAATC AGTGAGCAAC AGCTGGCAGA CCGTGGCAAA CCATGTAAAG
    ACCACCCTCT ATACTGTAAG AGGACTGCGG CCCAATACAA TCTACTTATT CATGGTCAGA
    GCGATCAACC CCCAAGGTCT CAGTGACCCA AGTCCCATGT CAGATCCTGT GCGCACACAA
    GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TGCAGAAAGA GCTAGGAGAT
    GTCCTTGTCC GTCTTCATAA TCCAGTTGTG CTGACTCCCA CCACGGTTCA GGTCACATGG
    ACGGTTGATC GCCAACCCCA GTTTATCCAA GGCTACCGAG TGATGTATCG TCAGACTTCA
    GGTCTGCAGG CGACATCTTC GTGGCAGAAT TTAGATGCCA AAGTCCCGAC TGAACGAAGT
    GCTGTCTTAG TCAACCTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTACG GCCATATTTT
    AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACGGTTC GTACTACTGA AGAAGCCCCA
    AGTGCCCCAC CACAGTCTGT CACTGTACTG ACAGTTGGAA GCTACAATAG CACAAGTATT
    AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CACCAGAATG GAATTATCCA AGAATACAAG
    ATCTGGTGTC TAGGAAATGA AACGCGATTC CATATCAACA AAACTGTGGA TGCAGCCATT
    CGGTCCGTAA TAATTGGTGG ATTATTCCCA GGTATTCAAT ACCGGGTAGA GGTTGCAGCT
    AGTACCAGTG CAGGGGTTGG AGTAAAGAGT GAGCCACAGC CAATAATAAT CGGGAGACGC
    AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTGGTG
    AAGCAACCAG CCTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT
    AGCATATGGT TGTATTGGCG AAGAAAGAAG AGGAAGGGAC TCAGTAATTA TGCTGTTACG
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GTCCAGGTCT TCTCAATGCT
    GGTGATCCCA GCTATCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGCTT GCCAGTAAAT
    AATAGCAACA GTGGCCCAAA TGAGATTGGA AATTTTGGCC GTGGAGATGT GCTGCCACCA
    GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGTAGC
    ATTGACTTCA CTACCAAAAC CAGTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA
    TATGCCACGA CACAGATCTT GCATTCCAAC AGCATACATG AATTGGCTGT CGATCTGCCT
    GATCCACAAT GGAAAAGCTC AATTCAGCAA AAAACAGATC TGATGGGATT TGGTTATTCT
    CTACCTGATC AGAACAAAGG TAACAATGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA
    AACTCTTCTA AACCACAGAA AAACAATGGA TCCACTTGGG CCAATGTCCC TCTACCTCCC
    CCCCCAGTCC AGCCCCTTCC TGGCACGGAG CTGGAACACT ATGCAGTGGA ACAACAAGAA
    AATGGCTATG ACAGTGATAG CTGGTGCCCA CCATTGCCAG TACAAACTTA CTTACACCAA
    GGTCTGGAAG ATGAACTGGA AGAAGATGAT GATAGGGTCC CAACACCTCC TGTTCGAGGC
    GTGGCTTCTT CTCCTGCTAT CTCCTTTGGA CAGCAGTCCA CTGCAACTCT TACTCCATCC
    CCACGGGAAG AGATGCAACC CATGCTGCAG GCTCACCTGG ATGAGTTGAC AAGAGCCTAT
    CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATAATCAACC TCCACAGCCT
    CCAGTTCCAC CGTTAGGTTA TGTGTCTGGA GCCTTGATTT CTGATTTGGA AACGGATGTT
    GCAGATGATG ATGCCGACGA CGAAGAGGAA GCTTTAGAAA TCCCCAGGCC CCTGAGAGCA
    CTGGACCAGA CTCCTGGATC CAGCATGGAC AATCTAGACA GCTCTGTGAC AGGAAAAGCC
    TTTACCTCCT CTCAAAGACC TCGACCTACC AGCCCATTTT CTACTGACAG TAACACCAGT
    GCAGCCCTGA GTCAAAGTCA GAGGCCTCGG CCCACTAAAA AACACAAGGG AGGGCGGATG
    GACCAACAAC CAGCATTGCC TCATCGAAGG GAAGGAATGA CAGATGAGGA GGCCTTGGTG
    CCCTATAGCA AGCCCAGTTT CCCATCTCCA GGTGGCCACA GCTCATCAGG AACAGCTTCT
    TCTAAGGGAT CCACTGGACC TAGGAAAACC GAGGTGTTGA GAGCAGGCCA CCAGCGCAAT
    GCCAGCGACC TTCTTGACAT AGGATATATG GGCTCCAACA GTCAAGGACA GTTTACAGGT
    GAATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001276968.1
    CDS644..4792
    Translation

    Target ORF information:

    RefSeq Version NM_001290039.1
    Organism Homo sapiens(human)
    Definition Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001290039.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
    GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC
    ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC
    ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC
    CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG
    AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG
    AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA
    GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC
    CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA
    ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG
    TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT
    GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA
    GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG
    GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT
    AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA
    AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCGCC CTGTTGCTCC CCCACAGTTT
    GTGGTTCGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA
    ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT
    TTCCCAAACC AACCCCAGCA GCCCAACAGT AGATGCTCAG TGTCACCAAC TGGAGACCTC
    ACAATCACCA ACATTCAACG TTCCGACGCG GGTTACTACA TCTGCCAGGC TTTAACTGTG
    GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAGGTTACTG ATGTTTTGAC AGATAGACCT
    CCACCTATAA TTCTACAAGG CCCAGCCAAC CAAACGCTGG CAGTGGATGG TACAGCGTTA
    CTGAAATGTA AAGCCACTGG TGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT
    ACTTTTCCGG GTAGAGATCC AAGAGCAACA ATTCAAGAGC AAGGCACACT GCAGATTAAG
    AATTTACGGA TTTCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGAGAG
    ACTTCCTGGA GTGCAGTGCT GGATGTGACA GAGTCTGGAG CAACAATCAG TAAAAACTAT
    GATTTAAGTG ACCTGCCAGG GCCACCATCC AAACCGCAGG TCACTGATGT TACTAAGAAC
    AGTGTCACCT TGTCCTGGCA GCCAGGTACC CCTGGAACCC TTCCAGCAAG TGCATATATC
    ATTGAGGCTT TCAGCCAATC AGTGAGCAAC AGCTGGCAGA CCGTGGCAAA CCATGTAAAG
    ACCACCCTCT ATACTGTAAG AGGACTGCGG CCCAATACAA TCTACTTATT CATGGTCAGA
    GCGATCAACC CCCAAGGTCT CAGTGACCCA AGTCCCATGT CAGATCCTGT GCGCACACAA
    GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TGCAGAAAGA GCTAGGAGAT
    GTCCTTGTCC GTCTTCATAA TCCAGTTGTG CTGACTCCCA CCACGGTTCA GGTCACATGG
    ACGGTTGATC GCCAACCCCA GTTTATCCAA GGCTACCGAG TGATGTATCG TCAGACTTCA
    GGTCTGCAGG CGACATCTTC GTGGCAGAAT TTAGATGCCA AAGTCCCGAC TGAACGAAGT
    GCTGTCTTAG TCAACCTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTACG GCCATATTTT
    AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACGGTTC GTACTACTGA AGAAGCCCCA
    AGTGCCCCAC CACAGTCTGT CACTGTACTG ACAGTTGGAA GCTACAATAG CACAAGTATT
    AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CACCAGAATG GAATTATCCA AGAATACAAG
    ATCTGGTGTC TAGGAAATGA AACGCGATTC CATATCAACA AAACTGTGGA TGCAGCCATT
    CGGTCCGTAA TAATTGGTGG ATTATTCCCA GGTATTCAAT ACCGGGTAGA GGTTGCAGCT
    AGTACCAGTG CAGGGGTTGG AGTAAAGAGT GAGCCACAGC CAATAATAAT CGGGAGACGC
    AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTGGTG
    AAGCAACCAG CCTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT
    AGCATATGGT TGTATTGGCG AAGAAAGAAG AGGAAGGGAC TCAGTAATTA TGCTGTTACG
    TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GTCCAGGTCT TCTCAATGCT
    GGTGATCCCA GCTATCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGCTT GCCAGTAAAT
    AATAGCAACA GTGGCCCAAA TGAGATTGGA AATTTTGGCC GTGGAGATGT GCTGCCACCA
    GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGTAGC
    ATTGACTTCA CTACCAAAAC CAGTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA
    TATGCCACGA CACAGATCTT GCATTCCAAC AGCATACATG AATTGGCTGT CGATCTGCCT
    GATCCACAAT GGAAAAGCTC AATTCAGCAA AAAACAGATC TGATGGGATT TGGTTATTCT
    CTACCTGATC AGAACAAAGG TAACAATGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA
    AACTCTTCTA AACCACAGAA AAACAATGGA TCCACTTGGG CCAATGTCCC TCTACCTCCC
    CCCCCAGTCC AGCCCCTTCC TGGCACGGAG CTGGAACACT ATGCAGTGGA ACAACAAGAA
    AATGGCTATG ACAGTGATAG CTGGTGCCCA CCATTGCCAG TACAAACTTA CTTACACCAA
    GGTCTGGAAG ATGAACTGGA AGAAGATGAT GATAGGGTCC CAACACCTCC TGTTCGAGGC
    GTGGCTTCTT CTCCTGCTAT CTCCTTTGGA CAGCAGTCCA CTGCAACTCT TACTCCATCC
    CCACGGGAAG AGATGCAACC CATGCTGCAG GCTCACCTGG ATGAGTTGAC AAGAGCCTAT
    CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATAATCAACC TCCACAGCCT
    CCAGTTCCAC CGTTAGGTTA TGTGTCTGGA GCCTTGATTT CTGATTTGGA AACGGATGTT
    GCAGATGATG ATGCCGACGA CGAAGAGGAA GCTTTAGAAA TCCCCAGGCC CCTGAGAGCA
    CTGGACCAGA CTCCTGGATC CAGCATGGAC AATCTAGACA GCTCTGTGAC AGGAAAAGCC
    TTTACCTCCT CTCAAAGACC TCGACCTACC AGCCCATTTT CTACTGACAG TAACACCAGT
    GCAGCCCTGA GTCAAAGTCA GAGGCCTCGG CCCACTAAAA AACACAAGGG AGGGCGGATG
    GACCAACAAC CAGCATTGCC TCATCGAAGG GAAGGAATGA CAGATGAGGA GGCCTTGGTG
    CCCTATAGCA AGCCCAGTTT CCCATCTCCA GGTGGCCACA GCTCATCAGG AACAGCTTCT
    TCTAAGGGAT CCACTGGACC TAGGAAAACC GAGGTGTTGA GAGCAGGCCA CCAGCGCAAT
    GCCAGCGACC TTCTTGACAT AGGATATATG GGCTCCAACA GTCAAGGACA GTTTACAGGT
    GAATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60280
    Clone ID Related Accession (Same CDS sequence) XM_011533981.2
    Accession Version XM_011533981.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4794bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product roundabout homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011533981.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
    CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT
    TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT
    CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC
    GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA
    AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC
    CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG
    TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA
    CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT
    CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC
    CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT
    GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT
    ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT
    AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC
    CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC
    ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG
    CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG
    GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT
    CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT
    GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG
    CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA
    GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG
    GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC
    GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT
    GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT
    CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG
    CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC
    GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT
    GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGCCT TACTTTACAT TCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCTCAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA ATAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCCAC TTGGGCCAAT
    GTCCCTCTAC CTCCCCCCCC AGTCCAGCCC CTTCCTGGCA CGGAGCTGGA ACACTATGCA
    GTGGAACAAC AAGAAAATGG CTATGACAGT GATAGCTGGT GCCCACCATT GCCAGTACAA
    ACTTACTTAC ACCAAGGTCT GGAAGATGAA CTGGAAGAAG ATGATGATAG GGTCCCAACA
    CCTCCTGTTC GAGGCGTGGC TTCTTCTCCT GCTATCTCCT TTGGACAGCA GTCCACTGCA
    ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAGGCTCA CCTGGATGAG
    TTGACAAGAG CCTATCAGTT TGATATAGCA AAACAAACAT GGCACATTCA AAGCAATAAT
    CAACCTCCAC AGCCTCCAGT TCCACCGTTA GGTTATGTGT CTGGAGCCTT GATTTCTGAT
    TTGGAAACGG ATGTTGCAGA TGATGATGCC GACGACGAAG AGGAAGCTTT AGAAATCCCC
    AGGCCCCTGA GAGCACTGGA CCAGACTCCT GGATCCAGCA TGGACAATCT AGACAGCTCT
    GTGACAGGTT CAATGGTAAA TGGATGGGGC TCTGCATCTG ATGAGGATCG TAACTTTTCT
    AGTCATAGAT CTAGTGTAGG TAGCTCCTCA GATGGCTCTA TCTTTGCCAG CGGCAGTTTT
    GCACAAGCAC TGGTGGCAGC AGCAGATAAA GCTGGTTTTA GGCTGGATGG AACCAGCCTT
    ACAAGAACAG GAAAAGCCTT TACCTCCTCT CAAAGACCTC GACCTACCAG CCCATTTTCT
    ACTGACAGTA ACACCAGTGC AGCCCTGAGT CAAAGTCAGA GGCCTCGGCC CACTAAAAAA
    CACAAGGGAG GGCGGATGGA CCAACAACCA GCATTGCCTC ATCGAAGGGA AGGAATGACA
    GATGATCTTC CACCACCACC AGATCCCCCG CCAGGTCAGG GTTTAAGGCA GCAAATAGGC
    CCGAGCCAGC AGGCTGGTAA CGTGGAAAAC TCAGCAGAGA GAAAAGGAAG CTCTCTAGAG
    AGACAACATG CATCCAGCTT AGAAGACACA AAGAGCTCAT TGGATTGTCC AGCTAGAACC
    TCCCTAGAGT GGCAGCGACA AACCCAGGAA TGGATAAGCT CCACAGAACG ACAAGAAGAT
    ATACGGAAAG CCCCACACAA ACAAGGTGTC GGATCAGAGG AGGCCTTGGT GCCCTATAGC
    AAGCCCAGTT TCCCATCTCC AGGTGGCCAC AGCTCATCAG GAACAGCTTC TTCTAAGGGA
    TCCACTGGAC CTAGGAAAAC CGAGGTGTTG AGAGCAGGCC ACCAGCGCAA TGCCAGCGAC
    CTTCTTGACA TAGGATATAT GGGCTCCAAC AGTCAAGGAC AGTTTACAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011532283.1
    CDS400..5193
    Translation

    Target ORF information:

    RefSeq Version XM_011533981.2
    Organism Homo sapiens(human)
    Definition Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011533981.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
    CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT
    TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT
    CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC
    GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA
    AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC
    CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG
    TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA
    CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT
    CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC
    CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT
    GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT
    ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT
    AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC
    CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC
    ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG
    CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG
    GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT
    CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT
    GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG
    CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA
    GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG
    GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC
    GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT
    GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT
    CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG
    CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC
    GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT
    GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGCCT TACTTTACAT TCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCTCAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA ATAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCCAC TTGGGCCAAT
    GTCCCTCTAC CTCCCCCCCC AGTCCAGCCC CTTCCTGGCA CGGAGCTGGA ACACTATGCA
    GTGGAACAAC AAGAAAATGG CTATGACAGT GATAGCTGGT GCCCACCATT GCCAGTACAA
    ACTTACTTAC ACCAAGGTCT GGAAGATGAA CTGGAAGAAG ATGATGATAG GGTCCCAACA
    CCTCCTGTTC GAGGCGTGGC TTCTTCTCCT GCTATCTCCT TTGGACAGCA GTCCACTGCA
    ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAGGCTCA CCTGGATGAG
    TTGACAAGAG CCTATCAGTT TGATATAGCA AAACAAACAT GGCACATTCA AAGCAATAAT
    CAACCTCCAC AGCCTCCAGT TCCACCGTTA GGTTATGTGT CTGGAGCCTT GATTTCTGAT
    TTGGAAACGG ATGTTGCAGA TGATGATGCC GACGACGAAG AGGAAGCTTT AGAAATCCCC
    AGGCCCCTGA GAGCACTGGA CCAGACTCCT GGATCCAGCA TGGACAATCT AGACAGCTCT
    GTGACAGGTT CAATGGTAAA TGGATGGGGC TCTGCATCTG ATGAGGATCG TAACTTTTCT
    AGTCATAGAT CTAGTGTAGG TAGCTCCTCA GATGGCTCTA TCTTTGCCAG CGGCAGTTTT
    GCACAAGCAC TGGTGGCAGC AGCAGATAAA GCTGGTTTTA GGCTGGATGG AACCAGCCTT
    ACAAGAACAG GAAAAGCCTT TACCTCCTCT CAAAGACCTC GACCTACCAG CCCATTTTCT
    ACTGACAGTA ACACCAGTGC AGCCCTGAGT CAAAGTCAGA GGCCTCGGCC CACTAAAAAA
    CACAAGGGAG GGCGGATGGA CCAACAACCA GCATTGCCTC ATCGAAGGGA AGGAATGACA
    GATGATCTTC CACCACCACC AGATCCCCCG CCAGGTCAGG GTTTAAGGCA GCAAATAGGC
    CCGAGCCAGC AGGCTGGTAA CGTGGAAAAC TCAGCAGAGA GAAAAGGAAG CTCTCTAGAG
    AGACAACATG CATCCAGCTT AGAAGACACA AAGAGCTCAT TGGATTGTCC AGCTAGAACC
    TCCCTAGAGT GGCAGCGACA AACCCAGGAA TGGATAAGCT CCACAGAACG ACAAGAAGAT
    ATACGGAAAG CCCCACACAA ACAAGGTGTC GGATCAGAGG AGGCCTTGGT GCCCTATAGC
    AAGCCCAGTT TCCCATCTCC AGGTGGCCAC AGCTCATCAG GAACAGCTTC TTCTAAGGGA
    TCCACTGGAC CTAGGAAAAC CGAGGTGTTG AGAGCAGGCC ACCAGCGCAA TGCCAGCGAC
    CTTCTTGACA TAGGATATAT GGGCTCCAAC AGTCAAGGAC AGTTTACAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60281
    Clone ID Related Accession (Same CDS sequence) XM_011533982.1
    Accession Version XM_011533982.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4674bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product roundabout homolog 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
    CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT
    TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT
    CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC
    GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA
    AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC
    CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG
    TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA
    CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT
    CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC
    CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT
    GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT
    ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT
    AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC
    CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC
    ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG
    CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG
    GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT
    CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT
    GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG
    CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA
    GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG
    GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC
    GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT
    GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT
    CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG
    CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC
    GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT
    GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGGTG GGAAAGGTGG AAAAAAGAAG
    AAAAATAAAA ACTCTTCTAA ACCACAGAAA AACAATGGAT CCACTTGGGC CAATGTCCCT
    CTACCTCCCC CCCCAGTCCA GCCCCTTCCT GGCACGGAGC TGGAACACTA TGCAGTGGAA
    CAACAAGAAA ATGGCTATGA CAGTGATAGC TGGTGCCCAC CATTGCCAGT ACAAACTTAC
    TTACACCAAG GTCTGGAAGA TGAACTGGAA GAAGATGATG ATAGGGTCCC AACACCTCCT
    GTTCGAGGCG TGGCTTCTTC TCCTGCTATC TCCTTTGGAC AGCAGTCCAC TGCAACTCTT
    ACTCCATCCC CACGGGAAGA GATGCAACCC ATGCTGCAGG CTCACCTGGA TGAGTTGACA
    AGAGCCTATC AGTTTGATAT AGCAAAACAA ACATGGCACA TTCAAAGCAA TAATCAACCT
    CCACAGCCTC CAGTTCCACC GTTAGGTTAT GTGTCTGGAG CCTTGATTTC TGATTTGGAA
    ACGGATGTTG CAGATGATGA TGCCGACGAC GAAGAGGAAG CTTTAGAAAT CCCCAGGCCC
    CTGAGAGCAC TGGACCAGAC TCCTGGATCC AGCATGGACA ATCTAGACAG CTCTGTGACA
    GGTTCAATGG TAAATGGATG GGGCTCTGCA TCTGATGAGG ATCGTAACTT TTCTAGTCAT
    AGATCTAGTG TAGGTAGCTC CTCAGATGGC TCTATCTTTG CCAGCGGCAG TTTTGCACAA
    GCACTGGTGG CAGCAGCAGA TAAAGCTGGT TTTAGGCTGG ATGGAACCAG CCTTACAAGA
    ACAGGAAAAG CCTTTACCTC CTCTCAAAGA CCTCGACCTA CCAGCCCATT TTCTACTGAC
    AGTAACACCA GTGCAGCCCT GAGTCAAAGT CAGAGGCCTC GGCCCACTAA AAAACACAAG
    GGAGGGCGGA TGGACCAACA ACCAGCATTG CCTCATCGAA GGGAAGGAAT GACAGATGAT
    CTTCCACCAC CACCAGATCC CCCGCCAGGT CAGGGTTTAA GGCAGCAAAT AGGCCCGAGC
    CAGCAGGCTG GTAACGTGGA AAACTCAGCA GAGAGAAAAG GAAGCTCTCT AGAGAGACAA
    CATGCATCCA GCTTAGAAGA CACAAAGAGC TCATTGGATT GTCCAGCTAG AACCTCCCTA
    GAGTGGCAGC GACAAACCCA GGAATGGATA AGCTCCACAG AACGACAAGA AGATATACGG
    AAAGCCCCAC ACAAACAAGG TGTCGGATCA GAGGAGGCCT TGGTGCCCTA TAGCAAGCCC
    AGTTTCCCAT CTCCAGGTGG CCACAGCTCA TCAGGAACAG CTTCTTCTAA GGGATCCACT
    GGACCTAGGA AAACCGAGGT GTTGAGAGCA GGCCACCAGC GCAATGCCAG CGACCTTCTT
    GACATAGGAT ATATGGGCTC CAACAGTCAA GGACAGTTTA CAGGTGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011532284.1
    CDS400..5073
    Translation

    Target ORF information:

    RefSeq Version XM_011533982.1
    Organism Homo sapiens(human)
    Definition Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011533982.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
    CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT
    TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT
    CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC
    GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA
    AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC
    CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG
    TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA
    CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT
    CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC
    CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT
    GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT
    ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT
    AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC
    CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC
    ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG
    CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG
    GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT
    CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT
    GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG
    CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA
    GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG
    GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC
    GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT
    GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT
    CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG
    CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC
    GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT
    GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGGTG GGAAAGGTGG AAAAAAGAAG
    AAAAATAAAA ACTCTTCTAA ACCACAGAAA AACAATGGAT CCACTTGGGC CAATGTCCCT
    CTACCTCCCC CCCCAGTCCA GCCCCTTCCT GGCACGGAGC TGGAACACTA TGCAGTGGAA
    CAACAAGAAA ATGGCTATGA CAGTGATAGC TGGTGCCCAC CATTGCCAGT ACAAACTTAC
    TTACACCAAG GTCTGGAAGA TGAACTGGAA GAAGATGATG ATAGGGTCCC AACACCTCCT
    GTTCGAGGCG TGGCTTCTTC TCCTGCTATC TCCTTTGGAC AGCAGTCCAC TGCAACTCTT
    ACTCCATCCC CACGGGAAGA GATGCAACCC ATGCTGCAGG CTCACCTGGA TGAGTTGACA
    AGAGCCTATC AGTTTGATAT AGCAAAACAA ACATGGCACA TTCAAAGCAA TAATCAACCT
    CCACAGCCTC CAGTTCCACC GTTAGGTTAT GTGTCTGGAG CCTTGATTTC TGATTTGGAA
    ACGGATGTTG CAGATGATGA TGCCGACGAC GAAGAGGAAG CTTTAGAAAT CCCCAGGCCC
    CTGAGAGCAC TGGACCAGAC TCCTGGATCC AGCATGGACA ATCTAGACAG CTCTGTGACA
    GGTTCAATGG TAAATGGATG GGGCTCTGCA TCTGATGAGG ATCGTAACTT TTCTAGTCAT
    AGATCTAGTG TAGGTAGCTC CTCAGATGGC TCTATCTTTG CCAGCGGCAG TTTTGCACAA
    GCACTGGTGG CAGCAGCAGA TAAAGCTGGT TTTAGGCTGG ATGGAACCAG CCTTACAAGA
    ACAGGAAAAG CCTTTACCTC CTCTCAAAGA CCTCGACCTA CCAGCCCATT TTCTACTGAC
    AGTAACACCA GTGCAGCCCT GAGTCAAAGT CAGAGGCCTC GGCCCACTAA AAAACACAAG
    GGAGGGCGGA TGGACCAACA ACCAGCATTG CCTCATCGAA GGGAAGGAAT GACAGATGAT
    CTTCCACCAC CACCAGATCC CCCGCCAGGT CAGGGTTTAA GGCAGCAAAT AGGCCCGAGC
    CAGCAGGCTG GTAACGTGGA AAACTCAGCA GAGAGAAAAG GAAGCTCTCT AGAGAGACAA
    CATGCATCCA GCTTAGAAGA CACAAAGAGC TCATTGGATT GTCCAGCTAG AACCTCCCTA
    GAGTGGCAGC GACAAACCCA GGAATGGATA AGCTCCACAG AACGACAAGA AGATATACGG
    AAAGCCCCAC ACAAACAAGG TGTCGGATCA GAGGAGGCCT TGGTGCCCTA TAGCAAGCCC
    AGTTTCCCAT CTCCAGGTGG CCACAGCTCA TCAGGAACAG CTTCTTCTAA GGGATCCACT
    GGACCTAGGA AAACCGAGGT GTTGAGAGCA GGCCACCAGC GCAATGCCAG CGACCTTCTT
    GACATAGGAT ATATGGGCTC CAACAGTCAA GGACAGTTTA CAGGTGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60282
    Clone ID Related Accession (Same CDS sequence) XM_011533983.1
    Accession Version XM_011533983.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4617bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product roundabout homolog 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
    CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT
    TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT
    CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC
    GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA
    AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC
    CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG
    TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA
    CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT
    CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC
    CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT
    GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT
    ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT
    AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC
    CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC
    ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG
    CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG
    GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT
    CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT
    GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG
    CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA
    GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG
    GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC
    GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT
    GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT
    CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG
    CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC
    GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT
    GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGCCT TACTTTACAT TCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCTCAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA ATAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCCAC TTGGGCCAAT
    GTCCCTCTAC CTCCCCCCCC AGTCCAGCCC CTTCCTGGCA CGGAGCTGGA ACACTATGCA
    GTGGAACAAC AAGAAAATGG CTATGACAGT GATAGCTGGT GCCCACCATT GCCAGTACAA
    ACTTACTTAC ACCAAGGTCT GGAAGATGAA CTGGAAGAAG ATGATGATAG GGTCCCAACA
    CCTCCTGTTC GAGGCGTGGC TTCTTCTCCT GCTATCTCCT TTGGACAGCA GTCCACTGCA
    ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAGGCTCA CCTGGATGAG
    TTGACAAGAG CCTATCAGTT TGATATAGCA AAACAAACAT GGCACATTCA AAGCAATAAT
    CAACCTCCAC AGCCTCCAGT TCCACCGTTA GGTTATGTGT CTGGAGCCTT GATTTCTGAT
    TTGGAAACGG ATGTTGCAGA TGATGATGCC GACGACGAAG AGGAAGCTTT AGAAATCCCC
    AGGCCCCTGA GAGCACTGGA CCAGACTCCT GGATCCAGCA TGGACAATCT AGACAGCTCT
    GTGACAGGAA AAGCCTTTAC CTCCTCTCAA AGACCTCGAC CTACCAGCCC ATTTTCTACT
    GACAGTAACA CCAGTGCAGC CCTGAGTCAA AGTCAGAGGC CTCGGCCCAC TAAAAAACAC
    AAGGGAGGGC GGATGGACCA ACAACCAGCA TTGCCTCATC GAAGGGAAGG AATGACAGAT
    GATCTTCCAC CACCACCAGA TCCCCCGCCA GGTCAGGGTT TAAGGCAGCA AATAGGCCCG
    AGCCAGCAGG CTGGTAACGT GGAAAACTCA GCAGAGAGAA AAGGAAGCTC TCTAGAGAGA
    CAACATGCAT CCAGCTTAGA AGACACAAAG AGCTCATTGG ATTGTCCAGC TAGAACCTCC
    CTAGAGTGGC AGCGACAAAC CCAGGAATGG ATAAGCTCCA CAGAACGACA AGAAGATATA
    CGGAAAGCCC CACACAAACA AGGTGTCGGA TCAGAGGAGG CCTTGGTGCC CTATAGCAAG
    CCCAGTTTCC CATCTCCAGG TGGCCACAGC TCATCAGGAA CAGCTTCTTC TAAGGGATCC
    ACTGGACCTA GGAAAACCGA GGTGTTGAGA GCAGGCCACC AGCGCAATGC CAGCGACCTT
    CTTGACATAG GATATATGGG CTCCAACAGT CAAGGACAGT TTACAGGTGA ATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011532285.1
    CDS400..5016
    Translation

    Target ORF information:

    RefSeq Version XM_011533983.1
    Organism Homo sapiens(human)
    Definition Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011533983.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
    CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT
    TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT
    CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC
    GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA
    AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC
    CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG
    TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA
    CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT
    CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC
    CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT
    GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT
    ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT
    AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC
    CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC
    ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG
    CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG
    GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT
    CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT
    GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG
    CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA
    GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG
    GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC
    GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT
    GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT
    CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG
    CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC
    GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT
    GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGCCT TACTTTACAT TCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCTCAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA ATAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCCAC TTGGGCCAAT
    GTCCCTCTAC CTCCCCCCCC AGTCCAGCCC CTTCCTGGCA CGGAGCTGGA ACACTATGCA
    GTGGAACAAC AAGAAAATGG CTATGACAGT GATAGCTGGT GCCCACCATT GCCAGTACAA
    ACTTACTTAC ACCAAGGTCT GGAAGATGAA CTGGAAGAAG ATGATGATAG GGTCCCAACA
    CCTCCTGTTC GAGGCGTGGC TTCTTCTCCT GCTATCTCCT TTGGACAGCA GTCCACTGCA
    ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAGGCTCA CCTGGATGAG
    TTGACAAGAG CCTATCAGTT TGATATAGCA AAACAAACAT GGCACATTCA AAGCAATAAT
    CAACCTCCAC AGCCTCCAGT TCCACCGTTA GGTTATGTGT CTGGAGCCTT GATTTCTGAT
    TTGGAAACGG ATGTTGCAGA TGATGATGCC GACGACGAAG AGGAAGCTTT AGAAATCCCC
    AGGCCCCTGA GAGCACTGGA CCAGACTCCT GGATCCAGCA TGGACAATCT AGACAGCTCT
    GTGACAGGAA AAGCCTTTAC CTCCTCTCAA AGACCTCGAC CTACCAGCCC ATTTTCTACT
    GACAGTAACA CCAGTGCAGC CCTGAGTCAA AGTCAGAGGC CTCGGCCCAC TAAAAAACAC
    AAGGGAGGGC GGATGGACCA ACAACCAGCA TTGCCTCATC GAAGGGAAGG AATGACAGAT
    GATCTTCCAC CACCACCAGA TCCCCCGCCA GGTCAGGGTT TAAGGCAGCA AATAGGCCCG
    AGCCAGCAGG CTGGTAACGT GGAAAACTCA GCAGAGAGAA AAGGAAGCTC TCTAGAGAGA
    CAACATGCAT CCAGCTTAGA AGACACAAAG AGCTCATTGG ATTGTCCAGC TAGAACCTCC
    CTAGAGTGGC AGCGACAAAC CCAGGAATGG ATAAGCTCCA CAGAACGACA AGAAGATATA
    CGGAAAGCCC CACACAAACA AGGTGTCGGA TCAGAGGAGG CCTTGGTGCC CTATAGCAAG
    CCCAGTTTCC CATCTCCAGG TGGCCACAGC TCATCAGGAA CAGCTTCTTC TAAGGGATCC
    ACTGGACCTA GGAAAACCGA GGTGTTGAGA GCAGGCCACC AGCGCAATGC CAGCGACCTT
    CTTGACATAG GATATATGGG CTCCAACAGT CAAGGACAGT TTACAGGTGA ATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60283
    Clone ID Related Accession (Same CDS sequence) XM_011533984.1
    Accession Version XM_011533984.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4344bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product roundabout homolog 2 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
    CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT
    TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT
    CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC
    GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA
    AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC
    CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG
    TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA
    CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT
    CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC
    CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT
    GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT
    ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT
    AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC
    CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC
    ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG
    CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG
    GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT
    CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT
    GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG
    CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA
    GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG
    GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC
    GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT
    GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT
    CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG
    CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC
    GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT
    GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGCCT TACTTTACAT TCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCTCAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA ATAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCCAC TTGGGCCAAT
    GTCCCTCTAC CTCCCCCCCC AGTCCAGCCC CTTCCTGGCA CGGAGCTGGA ACACTATGCA
    GTGGAACAAC AAGAAAATGG CTATGACAGT GATAGCTGGT GCCCACCATT GCCAGTACAA
    ACTTACTTAC ACCAAGGTCT GGAAGATGAA CTGGAAGAAG ATGATGATAG GGTCCCAACA
    CCTCCTGTTC GAGGCGTGGC TTCTTCTCCT GCTATCTCCT TTGGACAGCA GTCCACTGCA
    ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAGGCTCA CCTGGATGAG
    TTGACAAGAG CCTATCAGTT TGATATAGCA AAACAAACAT GGCACATTCA AAGCAATAAT
    CAACCTCCAC AGCCTCCAGT TCCACCGTTA GGTTATGTGT CTGGAGCCTT GATTTCTGAT
    TTGGAAACGG ATGTTGCAGA TGATGATGCC GACGACGAAG AGGAAGCTTT AGAAATCCCC
    AGGCCCCTGA GAGCACTGGA CCAGACTCCT GGATCCAGCA TGGACAATCT AGACAGCTCT
    GTGACAGGAA AAGCCTTTAC CTCCTCTCAA AGACCTCGAC CTACCAGCCC ATTTTCTACT
    GACAGTAACA CCAGTGCAGC CCTGAGTCAA AGTCAGAGGC CTCGGCCCAC TAAAAAACAC
    AAGGGAGGGC GGATGGACCA ACAACCAGCA TTGCCTCATC GAAGGGAAGG AATGACAGAT
    GAGGAGGCCT TGGTGCCCTA TAGCAAGCCC AGTTTCCCAT CTCCAGGTGG CCACAGCTCA
    TCAGGAACAG CTTCTTCTAA GGGATCCACT GGACCTAGGA AAACCGAGGT GTTGAGAGCA
    GGCCACCAGC GCAATGCCAG CGACCTTCTT GACATAGGAT ATATGGGCTC CAACAGTCAA
    GGACAGTTTA CAGGTGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011532286.1
    CDS400..4743
    Translation

    Target ORF information:

    RefSeq Version XM_011533984.1
    Organism Homo sapiens(human)
    Definition Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011533984.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
    CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT
    TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT
    CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC
    GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA
    AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC
    CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG
    TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA
    CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT
    CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC
    CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT
    GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT
    ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT
    AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC
    CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC
    ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG
    CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG
    GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT
    CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT
    GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG
    CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA
    GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG
    GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC
    GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT
    GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT
    CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG
    CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC
    GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT
    GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGCCT TACTTTACAT TCCTGACTAC
    CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCTCAGGA TTTCAGCACC
    ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA
    AAGAAGAAAA ATAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCCAC TTGGGCCAAT
    GTCCCTCTAC CTCCCCCCCC AGTCCAGCCC CTTCCTGGCA CGGAGCTGGA ACACTATGCA
    GTGGAACAAC AAGAAAATGG CTATGACAGT GATAGCTGGT GCCCACCATT GCCAGTACAA
    ACTTACTTAC ACCAAGGTCT GGAAGATGAA CTGGAAGAAG ATGATGATAG GGTCCCAACA
    CCTCCTGTTC GAGGCGTGGC TTCTTCTCCT GCTATCTCCT TTGGACAGCA GTCCACTGCA
    ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAGGCTCA CCTGGATGAG
    TTGACAAGAG CCTATCAGTT TGATATAGCA AAACAAACAT GGCACATTCA AAGCAATAAT
    CAACCTCCAC AGCCTCCAGT TCCACCGTTA GGTTATGTGT CTGGAGCCTT GATTTCTGAT
    TTGGAAACGG ATGTTGCAGA TGATGATGCC GACGACGAAG AGGAAGCTTT AGAAATCCCC
    AGGCCCCTGA GAGCACTGGA CCAGACTCCT GGATCCAGCA TGGACAATCT AGACAGCTCT
    GTGACAGGAA AAGCCTTTAC CTCCTCTCAA AGACCTCGAC CTACCAGCCC ATTTTCTACT
    GACAGTAACA CCAGTGCAGC CCTGAGTCAA AGTCAGAGGC CTCGGCCCAC TAAAAAACAC
    AAGGGAGGGC GGATGGACCA ACAACCAGCA TTGCCTCATC GAAGGGAAGG AATGACAGAT
    GAGGAGGCCT TGGTGCCCTA TAGCAAGCCC AGTTTCCCAT CTCCAGGTGG CCACAGCTCA
    TCAGGAACAG CTTCTTCTAA GGGATCCACT GGACCTAGGA AAACCGAGGT GTTGAGAGCA
    GGCCACCAGC GCAATGCCAG CGACCTTCTT GACATAGGAT ATATGGGCTC CAACAGTCAA
    GGACAGTTTA CAGGTGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60284
    Clone ID Related Accession (Same CDS sequence) XM_011533985.1
    Accession Version XM_011533985.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4218bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product roundabout homolog 2 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
    CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT
    TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT
    CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC
    GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA
    AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC
    CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG
    TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA
    CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT
    CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC
    CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT
    GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT
    ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT
    AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC
    CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC
    ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG
    CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG
    GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT
    CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT
    GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG
    CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA
    GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG
    GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC
    GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT
    GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT
    CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG
    CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC
    GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT
    GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGGTG GGAAAGGTGG AAAAAAGAAG
    AAAAATAAAA ACTCTTCTAA ACCACAGAAA AACAATGGAT CCACTTGGGC CAATGTCCCT
    CTACCTCCCC CCCCAGTCCA GCCCCTTCCT GGCACGGAGC TGGAACACTA TGCAGTGGAA
    CAACAAGAAA ATGGCTATGA CAGTGATAGC TGGTGCCCAC CATTGCCAGT ACAAACTTAC
    TTACACCAAG GTCTGGAAGA TGAACTGGAA GAAGATGATG ATAGGGTCCC AACACCTCCT
    GTTCGAGGCG TGGCTTCTTC TCCTGCTATC TCCTTTGGAC AGCAGTCCAC TGCAACTCTT
    ACTCCATCCC CACGGGAAGA GATGCAACCC ATGCTGCAGG CTCACCTGGA TGAGTTGACA
    AGAGCCTATC AGTTTGATAT AGCAAAACAA ACATGGCACA TTCAAAGCAA TAATCAACCT
    CCACAGCCTC CAGTTCCACC GTTAGGTTAT GTGTCTGGAG CCTTGATTTC TGATTTGGAA
    ACGGATGTTG CAGATGATGA TGCCGACGAC GAAGAGGAAG CTTTAGAAAT CCCCAGGCCC
    CTGAGAGCAC TGGACCAGAC TCCTGGATCC AGCATGGACA ATCTAGACAG CTCTGTGACA
    GGAAAAGCCT TTACCTCCTC TCAAAGACCT CGACCTACCA GCCCATTTTC TACTGACAGT
    AACACCAGTG CAGCCCTGAG TCAAAGTCAG AGGCCTCGGC CCACTAAAAA ACACAAGGGA
    GGGCGGATGG ACCAACAACC AGCATTGCCT CATCGAAGGG AAGGAATGAC AGATGAGGAG
    GCCTTGGTGC CCTATAGCAA GCCCAGTTTC CCATCTCCAG GTGGCCACAG CTCATCAGGA
    ACAGCTTCTT CTAAGGGATC CACTGGACCT AGGAAAACCG AGGTGTTGAG AGCAGGCCAC
    CAGCGCAATG CCAGCGACCT TCTTGACATA GGATATATGG GCTCCAACAG TCAAGGACAG
    TTTACAGGTG AATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011532287.1
    CDS400..4617
    Translation

    Target ORF information:

    RefSeq Version XM_011533985.1
    Organism Homo sapiens(human)
    Definition Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011533985.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
    CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT
    TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT
    TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG
    CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT
    CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC
    GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT
    GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA
    AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC
    CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG
    GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT
    GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA
    GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG
    GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG
    TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA
    CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT
    CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC
    CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT
    CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG
    AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT
    GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT
    TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA
    GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT
    ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG
    GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG
    CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA
    AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT
    AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT
    ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT
    GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC
    CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC
    ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG
    CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG
    CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG
    GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT
    CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT
    GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG
    CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA
    GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC
    ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA
    GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT
    GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG
    GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC
    GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT
    GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG
    ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT
    GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT
    CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG
    CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG
    CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT
    TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG
    GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC
    GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT
    GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGGTG GGAAAGGTGG AAAAAAGAAG
    AAAAATAAAA ACTCTTCTAA ACCACAGAAA AACAATGGAT CCACTTGGGC CAATGTCCCT
    CTACCTCCCC CCCCAGTCCA GCCCCTTCCT GGCACGGAGC TGGAACACTA TGCAGTGGAA
    CAACAAGAAA ATGGCTATGA CAGTGATAGC TGGTGCCCAC CATTGCCAGT ACAAACTTAC
    TTACACCAAG GTCTGGAAGA TGAACTGGAA GAAGATGATG ATAGGGTCCC AACACCTCCT
    GTTCGAGGCG TGGCTTCTTC TCCTGCTATC TCCTTTGGAC AGCAGTCCAC TGCAACTCTT
    ACTCCATCCC CACGGGAAGA GATGCAACCC ATGCTGCAGG CTCACCTGGA TGAGTTGACA
    AGAGCCTATC AGTTTGATAT AGCAAAACAA ACATGGCACA TTCAAAGCAA TAATCAACCT
    CCACAGCCTC CAGTTCCACC GTTAGGTTAT GTGTCTGGAG CCTTGATTTC TGATTTGGAA
    ACGGATGTTG CAGATGATGA TGCCGACGAC GAAGAGGAAG CTTTAGAAAT CCCCAGGCCC
    CTGAGAGCAC TGGACCAGAC TCCTGGATCC AGCATGGACA ATCTAGACAG CTCTGTGACA
    GGAAAAGCCT TTACCTCCTC TCAAAGACCT CGACCTACCA GCCCATTTTC TACTGACAGT
    AACACCAGTG CAGCCCTGAG TCAAAGTCAG AGGCCTCGGC CCACTAAAAA ACACAAGGGA
    GGGCGGATGG ACCAACAACC AGCATTGCCT CATCGAAGGG AAGGAATGAC AGATGAGGAG
    GCCTTGGTGC CCTATAGCAA GCCCAGTTTC CCATCTCCAG GTGGCCACAG CTCATCAGGA
    ACAGCTTCTT CTAAGGGATC CACTGGACCT AGGAAAACCG AGGTGTTGAG AGCAGGCCAC
    CAGCGCAATG CCAGCGACCT TCTTGACATA GGATATATGG GCTCCAACAG TCAAGGACAG
    TTTACAGGTG AATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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