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RPS13 cDNA ORF clone, Homo sapiens (human)

Gene Symbol RPS13
Entrez Gene ID 6207
Full Name ribosomal protein S13
Synonyms S13
General protein information
Preferred Names
40S ribosomal protein S13
Names
40S ribosomal protein S13
NP_001008.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11p15

Summary Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S15P family of ribosomal proteins. It is located in the cytoplasm. The protein has been shown to bind to the 5.8S rRNA in rat. The gene product of the E. coli ortholog (ribosomal protein S15) functions at early steps in ribosome assembly. This gene is co-transcribed with two U14 small nucleolar RNA genes, which are located in its third and fifth introns. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

180476


mRNA and Protein(s)

mRNA Protein Name
NM_001017 NP_001008 40S ribosomal protein S13



Homo sapiens (human) RPS13 NP_001008.1
Pan troglodytes (chimpanzee) RPS13 XP_003312983.1
Macaca mulatta (Rhesus monkey) RPS13 XP_001086088.1
Canis lupus familiaris (dog) RPS13 XP_864902.1
Bos taurus (cattle) RPS13 NP_001020513.1
Mus musculus (house mouse) Rps13 NP_080809.1
Gallus gallus (chicken) RPS13 NP_001001783.1
Danio rerio (zebrafish) rps13 NP_001002079.1
Drosophila melanogaster (fruit fly) RpS13 NP_476938.1
Caenorhabditis elegans rps-13 NP_498393.1
Arabidopsis thaliana (thale cress) AT3G60770 NP_567104.1
Arabidopsis thaliana (thale cress) RPS13A NP_567151.1
Xenopus (Silurana) tropicalis (western clawed frog) rps13 NP_001016602.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RPS13 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPS13 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu13042
NM_001017 Homo sapiens ribosomal protein S13 (RPS13), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu13042
Clone ID Related Accession (Same CDS sequence) NM_001017
Accession Version NM_001017.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 456bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product 40S ribosomal protein S13
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from L01124.1. On Jul 3, 2001 this sequence version replaced gi:4506684. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BQ277838.1, AV701531.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
ATGGGTCGCA TGCATGCTCC CGGGAAGGGC CTGTCCCAGT CGGCTTTACC CTATCGACGC 
AGCGTCCCCA CTTGGTTGAA GTTGACATCT GACGACGTGA AGGAGCAGAT TTACAAACTG
GCCAAGAAGG GCCTTACTCC TTCACAGATC GGTGTAATCC TGAGAGATTC ACATGGTGTT
GCACAAGTAC GTTTTGTGAC AGGCAATAAA ATTTTAAGAA TTCTTAAGTC TAAGGGACTT
GCTCCTGATC TTCCTGAAGA TCTCTACCAT TTAATTAAGA AAGCAGTTGC TGTTCGAAAG
CATCTTGAGA GGAACAGAAA GGATAAGGAT GCTAAATTCC GTCTGATTCT AATAGAGAGC
CGGATTCACC GTTTGGCTCG ATATTATAAG ACCAAGCGAG TCCTCCCTCC CAATTGGAAA
TATGAATCAT CTACAGCCTC TGCCCTGGTC GCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001008.1
CDS33..488
Misc Feature(1)1..1(+)
Misc Feature(2)7..7(+)
Misc Feature(3)33..212(+)
Misc Feature(4)42..485(+)
Misc Feature(5)111..113(+)
Misc Feature(6)144..146(+)
Misc Feature(7)144..146(+)
Misc Feature(8)240..467(+)
Misc Feature(9)243..416(+)
Misc Feature(10)297..461(+)
Misc Feature(11)387..467(+)
Misc Feature(12)414..416(+)
Misc Feature(13)417..419(+)
Exon (1)1..55
Gene:RPS13
Gene Synonym:
Exon (2)56..104
Gene:RPS13
Gene Synonym:
Exon (3)105..183
Gene:RPS13
Gene Synonym:
Exon (4)184..353
Gene:RPS13
Gene Synonym:
Exon (5)354..454
Gene:RPS13
Gene Synonym:
Exon (6)455..529
Gene:RPS13
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001017
Organism Homo sapiens (human)
Definition Homo sapiens ribosomal protein S13 (RPS13), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001017

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGGGTCGCA TGCATGCTCC CGGGAAGGGC CTGTCCCAGT CGGCTTTACC CTATCGACGC 
AGCGTCCCCA CTTGGTTGAA GTTGACATCT GACGACGTGA AGGAGCAGAT TTACAAACTG
GCCAAGAAGG GCCTTACTCC TTCACAGATC GGTGTAATCC TGAGAGATTC ACATGGTGTT
GCACAAGTAC GTTTTGTGAC AGGCAATAAA ATTTTAAGAA TTCTTAAGTC TAAGGGACTT
GCTCCTGATC TTCCTGAAGA TCTCTACCAT TTAATTAAGA AAGCAGTTGC TGTTCGAAAG
CATCTTGAGA GGAACAGAAA GGATAAGGAT GCTAAATTCC GTCTGATTCT AATAGAGAGC
CGGATTCACC GTTTGGCTCG ATATTATAAG ACCAAGCGAG TCCTCCCTCC CAATTGGAAA
TATGAATCAT CTACAGCCTC TGCCCTGGTC GCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

[Binding of human ribosomal protein S13 to the central domain of 18S rRNA]
Mol. Biol. (Mosk.) 45 (6), 1046-1054 (2011)
Ivanov,A.V., Malygin,A.A. and Karpova,G.G.


book

Human ribosomal protein S13 promotes gastric cancer growth through down-regulating p27(Kip1)
J. Cell. Mol. Med. 15 (2), 296-306 (2011)
Guo X, Shi Y, Gou Y, Li J, Han S, Zhang Y, Huo J, Ning X, Sun L, Chen Y, Sun S and Fan D.


book

Human ribosomal protein S13 regulates expression of its own gene at the splicing step by a feedback mechanism
Nucleic Acids Res. 35 (19), 6414-6423 (2007)
Malygin AA, Parakhnevitch NM, Ivanov AV, Eperon IC and Karpova GG.


book

Mass spectrometric analysis of the human 40S ribosomal subunit: native and HCV IRES-bound complexes
Protein Sci. 14 (6), 1438-1446 (2005)
Yu Y, Ji H, Doudna JA and Leary JA.


book

Human ribosomal protein S13: cloning, expression, refolding, and structural stability
Biochim. Biophys. Acta 1747 (1), 93-97 (2005)
Malygin A, Parakhnevitch N and Karpova G.


book

Characterization of the human small-ribosomal-subunit proteins by N-terminal and internal sequencing, and mass spectrometry
Eur. J. Biochem. 239 (1), 144-149 (1996)
Vladimirov SN, Ivanov AV, Karpova GG, Musolyamov AK, Egorov TA, Thiede B, Wittmann-Liebold B and Otto A.


book

Structure and evolution of mammalian ribosomal proteins
Biochem. Cell Biol. 73 (11-12), 933-947 (1995)
Wool IG, Chan YL and Gluck A.


book

Construction of a human full-length cDNA bank
Gene 150 (2), 243-250 (1994)
Kato S, Sekine S, Oh SW, Kim NS, Umezawa Y, Abe N, Yokoyama-Kobayashi M and Aoki T.


book

Cloning and analysis of the human S13 ribosomal protein cDNA
Nucleic Acids Res. 21 (12), 2945 (1993)
Chadeneau C, LeMoullac B and Denis MG.


book

The primary structure of rat ribosomal protein S13
Biochem. Biophys. Res. Commun. 171 (2), 519-524 (1990)
Suzuki K, Olvera J and Wool IG.


 
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