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SDHA cDNA ORF clone, Homo sapiens(Human)

Gene Symbol SDHA
Entrez Gene ID 6389
Full Name succinate dehydrogenase complex flavoprotein subunit A
Synonyms CMD1GG,FP,PGL5,SDH1,SDH2,SDHF
General protein information
Preferred Names

succinate dehydrogenase complex flavoprotein subunit A

Names

succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
flavoprotein subunit of complex II
succinate dehydrogenase complex, subunit A, flavoprotein (Fp)

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

5

5p15.33

Summary This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014].
Disorder MIM:

600857

Disorder Html: Leigh syndrome, 256000 (3); Mitochondrial respiratory chain complex

mRNA and Protein(s)

mRNA Protein Name
XM_017009685.1 XP_016865174.1 succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial isoform X1
NM_001330758.1 NP_001317687.1 succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial isoform 3
XM_011514073.1 XP_011512375.1 succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial isoform X3
XM_011514072.1 XP_011512374.1 succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial isoform X2
NM_004168.3 NP_004159.2 succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial isoform 1
NM_001294332.1 NP_001281261.1 succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial isoform 2



Gallus gallus (chicken) SDHA NP_001264327.1
Drosophila melanogaster (fruit fly) SdhA NP_725882.1
Eremothecium gossypii AGOS_ACR052W NP_983455.2
Schizosaccharomyces pombe (fission yeast) sdh1 NP_594319.1
Oryza sativa (rice) Os07g0134800 NP_001058845.1
Bos taurus (cattle) SDHA NP_776603.1
Mus musculus (house mouse) Sdha NP_075770.1
Rattus norvegicus (Norway rat) Sdha NP_569112.1
Danio rerio (zebrafish) sdha NP_957204.1
Caenorhabditis elegans (roundworm) sdha-2 NP_492798.1
Arabidopsis thaliana (thale cress) SDH1-1 NP_201477.1
Saccharomyces cerevisiae (baker's yeast) SDH1 NP_012774.1
Kluyveromyces lactis KLLA0D04444g XP_453260.1
Neurospora crassa NCU08336 XP_001728186.1
Pan troglodytes (chimpanzee) SDHA XP_003310656.1
Canis lupus familiaris (dog) SDHA XP_535807.4
Anopheles gambiae (African malaria mosquito) AgaP_AGAP010429 XP_311518.4
Magnaporthe oryzae (rice blast fungus) MGG_00168 XP_003718957.1
Xenopus tropicalis (tropical clawed frog) sdha NP_001015989.1
Macaca mulatta (rhesus monkey) LOC708018 XP_001094170.2
Homo sapiens (human) SDHA NP_004159.2
Caenorhabditis elegans (roundworm) sdha-1 NP_509446.1


Related articles in PubMed

Cancer-associated fibroblasts enhance pancreatic cancer cell invasion by remodeling the metabolic conversion mechanism.
Shan T, Chen S, Chen X, Lin WR, Li W, Ma J, Wu T, Cui X, Ji H, Li Y, Kang Y
Oncology reports37(4)1971-1979(2017 Apr)

Potential Pitfalls of SDH Immunohistochemical Detection in Paragangliomas and Phaeochromocytomas Harbouring Germline
Santi R, Rapizzi E, Canu L, Ercolino T, Baroni G, Fucci R, Costa G, Mannelli M, Nesi G
Anticancer research37(2)805-812(2017 02)

Inhibition of succinate dehydrogenase sensitizes cyclin E-driven ovarian cancer to CDK inhibition.
Guo T, Gu C, Chen X, Kang Y, Li B, Xu C
BioFactors (Oxford, England)42(2)171-8(2016 Mar-Apr)

Cardiac-specific succinate dehydrogenase deficiency in Barth syndrome.
Dudek J, Cheng IF, Chowdhury A, Wozny K, Balleininger M, Reinhold R, Grunau S, Callegari S, Toischer K, Wanders RJ, Hasenfuß G, Brügger B, Guan K, Rehling P
EMBO molecular medicine8(2)139-54(2016 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

After indirect co-culture, OP was increased in the BxPc-3 and Panc-1 cells; correspondingly, succinate dehydrogenase, FH and MCT expression were increased. After the MCT1-specific inhibitor removed 'tumor-stromal' metabolic coupling, the migration and invasion abilities of the pancreatic cancer cells were decreased.
Title: Cancer-associated fibroblasts enhance pancreatic cancer cell invasion by remodeling the metabolic conversion mechanism.

Data suggest that succinate dehydrogenases SDHA and SDHB immunohistochemistry should be interpreted with caution, due to possible false-positive or false-negative results, and ideally in the setting of quality assurance provided by molecular testing.
Title: Potential Pitfalls of SDH Immunohistochemical Detection in Paragangliomas and Phaeochromocytomas Harbouring Germline

SDH-deficient gastrointestinal stromal tumors (GISTs) account for approximately 8% of gastric GISTs and are associated with a high rate of distant metastasis, regardless of conventional risk category.
Title: Conventional Risk Stratification Fails to Predict Progression of Succinate Dehydrogenase-deficient Gastrointestinal Stromal Tumors: A Clinicopathologic Study of 76 Cases.

data show that SDHA flavination is independent of SDHAF2 in breast cancer cells, employing an alternative mechanism.
Title: The Assembly Factor SDHAF2 Is Dispensable for Flavination of the Catalytic Subunit of Mitochondrial Complex II in Breast Cancer Cells.

FAD interacts noncovalently with SDHA in the absence of SDH5
Title: In-vitro, SDH5-dependent flavinylation of immobilized human respiratory complex II flavoprotein.

The following SDHA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SDHA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu28692
NM_004168.3
Latest version!
Homo sapiens succinate dehydrogenase complex flavoprotein subunit A (SDHA), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu28693 NM_001294332.1
Latest version!
Homo sapiens succinate dehydrogenase complex flavoprotein subunit A (SDHA), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu39348 NM_001330758.1
Latest version!
Homo sapiens succinate dehydrogenase complex flavoprotein subunit A (SDHA), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu60441 XM_011514073.1
Latest version!
Homo sapiens succinate dehydrogenase complex flavoprotein subunit A (SDHA), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu60440 XM_011514072.1
Latest version!
Homo sapiens succinate dehydrogenase complex flavoprotein subunit A (SDHA), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu95977 XM_017009685.1
Latest version!
Homo sapiens succinate dehydrogenase complex flavoprotein subunit A (SDHA), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu28692
Clone ID Related Accession (Same CDS sequence) NM_004168.3
Accession Version NM_004168.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1995bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Homo sapiens(Human)
Product succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA160919.1, BC001380.2, AK291311.1, AC021087.5 and HY265959.1. This sequence is a reference standard in the RefSeqGene project. On Jul 1, 2014 this sequence version replaced NM_004168.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.168940.1, SRR1803617.36814.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGTCGGGGG TCCGGGGCCT GTCGCGGCTG CTGAGCGCTC GGCGCCTGGC GCTGGCCAAG 
GCGTGGCCAA CAGTGTTGCA AACAGGAACC CGAGGTTTTC ACTTCACTGT TGATGGGAAC
AAGAGGGCAT CTGCTAAAGT TTCAGATTCC ATTTCTGCTC AGTATCCAGT AGTGGATCAT
GAATTTGATG CAGTGGTGGT AGGCGCTGGA GGGGCAGGCT TGCGAGCTGC ATTTGGCCTT
TCTGAGGCAG GGTTTAATAC AGCATGTGTT ACCAAGCTGT TTCCTACCAG GTCACACACT
GTTGCAGCAC AGGGAGGAAT CAATGCTGCT CTGGGGAACA TGGAGGAGGA CAACTGGAGG
TGGCATTTCT ACGACACCGT GAAGGGCTCC GACTGGCTGG GGGACCAGGA TGCCATCCAC
TACATGACGG AGCAGGCCCC CGCCGCCGTG GTCGAGCTAG AAAATTATGG CATGCCGTTT
AGCAGAACTG AAGATGGGAA GATTTATCAG CGTGCATTTG GTGGACAGAG CCTCAAGTTT
GGAAAGGGCG GGCAGGCCCA TCGGTGCTGC TGTGTGGCTG ATCGGACTGG CCACTCGCTA
TTGCACACCT TATATGGAAG GTCTCTGCGA TATGATACCA GCTATTTTGT GGAGTATTTT
GCCTTGGATC TCCTGATGGA GAATGGGGAG TGCCGTGGTG TCATCGCACT GTGCATAGAG
GACGGGTCCA TCCATCGCAT AAGAGCAAAG AACACTGTTG TTGCCACAGG AGGCTACGGG
CGCACCTACT TCAGCTGCAC GTCTGCCCAC ACCAGCACTG GCGACGGCAC GGCCATGATC
ACCAGGGCAG GCCTTCCTTG CCAGGACCTA GAGTTTGTTC AGTTCCACCC TACAGGCATA
TATGGTGCTG GTTGTCTCAT TACGGAAGGA TGTCGTGGAG AGGGAGGCAT TCTCATTAAC
AGTCAAGGCG AAAGGTTTAT GGAGCGATAC GCCCCTGTCG CGAAGGACCT GGCGTCTAGA
GATGTGGTGT CTCGGTCCAT GACTCTGGAG ATCCGAGAAG GAAGAGGCTG TGGCCCTGAG
AAAGATCACG TCTACCTGCA GCTGCACCAC CTACCTCCAG AGCAGCTGGC CACGCGCCTG
CCTGGCATTT CAGAGACAGC CATGATCTTC GCTGGCGTGG ACGTCACGAA GGAGCCGATC
CCTGTCCTCC CCACCGTGCA TTATAACATG GGCGGCATTC CCACCAACTA CAAGGGGCAG
GTCCTGAGGC ACGTGAATGG CCAGGATCAG ATTGTGCCCG GCCTGTACGC CTGTGGGGAG
GCCGCCTGTG CCTCGGTACA TGGTGCCAAC CGCCTCGGGG CAAACTCGCT CTTGGACCTG
GTTGTCTTTG GTCGGGCATG TGCCCTGAGC ATCGAAGAGT CATGCAGGCC TGGAGATAAA
GTCCCTCCAA TTAAACCAAA CGCTGGGGAA GAATCTGTCA TGAATCTTGA CAAATTGAGA
TTTGCTGATG GAAGCATAAG AACATCGGAA CTGCGACTCA GCATGCAGAA GTCAATGCAA
AATCATGCTG CCGTGTTCCG TGTGGGAAGC GTGTTGCAAG AAGGTTGTGG GAAAATCAGC
AAGCTCTATG GAGACCTAAA GCACCTGAAG ACGTTCGACC GGGGAATGGT CTGGAACACG
GACCTGGTGG AGACCCTGGA GCTGCAGAAC CTGATGCTGT GTGCGCTGCA GACCATCTAC
GGAGCAGAGG CACGGAAGGA GTCACGGGGC GCGCATGCCA GGGAAGACTA CAAGGTGCGG
ATTGATGAGT ACGATTACTC CAAGCCCATC CAGGGGCAAC AGAAGAAGCC CTTTGAGGAG
CACTGGAGGA AGCACACCCT GTCCTATGTG GACGTTGGCA CTGGGAAGGT CACTCTGGAA
TATAGACCCG TGATCGACAA AACTTTGAAC GAGGCTGACT GTGCCACCGT CCCGCCAGCC
ATTCGCTCCT ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_004159.2
CDS134..2128
Misc Feature(1)251..2125
Misc Feature(2)386..1546
Misc Feature(3)428..430
Misc Feature(4)632..634
Misc Feature(5)668..670
Misc Feature(6)668..670
Misc Feature(7)677..679
Misc Feature(8)776..778
Misc Feature(9)881..883
Misc Feature(10)881..883
Misc Feature(11)1136..1138
Misc Feature(12)1136..1138
Misc Feature(13)1571..1573
Misc Feature(14)1586..1588
Misc Feature(15)1586..1588
Misc Feature(16)1625..1627
Misc Feature(17)1625..1627
Misc Feature(18)1676..2125
Misc Feature(19)1682..1684
Misc Feature(20)1745..1747
Misc Feature(21)1745..1747
Misc Feature(22)1754..1756
Misc Feature(23)1772..1774
Misc Feature(24)1772..1774
Misc Feature(25)1781..1783
Misc Feature(26)1925..1927
Misc Feature(27)1955..1957
Misc Feature(28)1976..1978
Misc Feature(29)2003..2005
Misc Feature(30)2039..2041
Misc Feature(31)2072..2074
Exon (1)1..196
Exon (2)197..283
Exon (3)284..445
Exon (4)446..589
Exon (5)590..754
Exon (6)755..903
Exon (7)904..1028
Exon (8)1029..1197
Exon (9)1198..1393
Exon (10)1394..1565
Exon (11)1566..1684
Exon (12)1685..1796
Exon (13)1797..1927
Exon (14)1928..2041
Exon (15)2042..2790
Translation

Target ORF information:

RefSeq Version NM_004168.3
Organism Homo sapiens(Human)
Definition Homo sapiens succinate dehydrogenase complex flavoprotein subunit A (SDHA), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_004168.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGTCGGGGG TCCGGGGCCT GTCGCGGCTG CTGAGCGCTC GGCGCCTGGC GCTGGCCAAG 
GCGTGGCCAA CAGTGTTGCA AACAGGAACC CGAGGTTTTC ACTTCACTGT TGATGGGAAC
AAGAGGGCAT CTGCTAAAGT TTCAGATTCC ATTTCTGCTC AGTATCCAGT AGTGGATCAT
GAATTTGATG CAGTGGTGGT AGGCGCTGGA GGGGCAGGCT TGCGAGCTGC ATTTGGCCTT
TCTGAGGCAG GGTTTAATAC AGCATGTGTT ACCAAGCTGT TTCCTACCAG GTCACACACT
GTTGCAGCAC AGGGAGGAAT CAATGCTGCT CTGGGGAACA TGGAGGAGGA CAACTGGAGG
TGGCATTTCT ACGACACCGT GAAGGGCTCC GACTGGCTGG GGGACCAGGA TGCCATCCAC
TACATGACGG AGCAGGCCCC CGCCGCCGTG GTCGAGCTAG AAAATTATGG CATGCCGTTT
AGCAGAACTG AAGATGGGAA GATTTATCAG CGTGCATTTG GTGGACAGAG CCTCAAGTTT
GGAAAGGGCG GGCAGGCCCA TCGGTGCTGC TGTGTGGCTG ATCGGACTGG CCACTCGCTA
TTGCACACCT TATATGGAAG GTCTCTGCGA TATGATACCA GCTATTTTGT GGAGTATTTT
GCCTTGGATC TCCTGATGGA GAATGGGGAG TGCCGTGGTG TCATCGCACT GTGCATAGAG
GACGGGTCCA TCCATCGCAT AAGAGCAAAG AACACTGTTG TTGCCACAGG AGGCTACGGG
CGCACCTACT TCAGCTGCAC GTCTGCCCAC ACCAGCACTG GCGACGGCAC GGCCATGATC
ACCAGGGCAG GCCTTCCTTG CCAGGACCTA GAGTTTGTTC AGTTCCACCC TACAGGCATA
TATGGTGCTG GTTGTCTCAT TACGGAAGGA TGTCGTGGAG AGGGAGGCAT TCTCATTAAC
AGTCAAGGCG AAAGGTTTAT GGAGCGATAC GCCCCTGTCG CGAAGGACCT GGCGTCTAGA
GATGTGGTGT CTCGGTCCAT GACTCTGGAG ATCCGAGAAG GAAGAGGCTG TGGCCCTGAG
AAAGATCACG TCTACCTGCA GCTGCACCAC CTACCTCCAG AGCAGCTGGC CACGCGCCTG
CCTGGCATTT CAGAGACAGC CATGATCTTC GCTGGCGTGG ACGTCACGAA GGAGCCGATC
CCTGTCCTCC CCACCGTGCA TTATAACATG GGCGGCATTC CCACCAACTA CAAGGGGCAG
GTCCTGAGGC ACGTGAATGG CCAGGATCAG ATTGTGCCCG GCCTGTACGC CTGTGGGGAG
GCCGCCTGTG CCTCGGTACA TGGTGCCAAC CGCCTCGGGG CAAACTCGCT CTTGGACCTG
GTTGTCTTTG GTCGGGCATG TGCCCTGAGC ATCGAAGAGT CATGCAGGCC TGGAGATAAA
GTCCCTCCAA TTAAACCAAA CGCTGGGGAA GAATCTGTCA TGAATCTTGA CAAATTGAGA
TTTGCTGATG GAAGCATAAG AACATCGGAA CTGCGACTCA GCATGCAGAA GTCAATGCAA
AATCATGCTG CCGTGTTCCG TGTGGGAAGC GTGTTGCAAG AAGGTTGTGG GAAAATCAGC
AAGCTCTATG GAGACCTAAA GCACCTGAAG ACGTTCGACC GGGGAATGGT CTGGAACACG
GACCTGGTGG AGACCCTGGA GCTGCAGAAC CTGATGCTGT GTGCGCTGCA GACCATCTAC
GGAGCAGAGG CACGGAAGGA GTCACGGGGC GCGCATGCCA GGGAAGACTA CAAGGTGCGG
ATTGATGAGT ACGATTACTC CAAGCCCATC CAGGGGCAAC AGAAGAAGCC CTTTGAGGAG
CACTGGAGGA AGCACACCCT GTCCTATGTG GACGTTGGCA CTGGGAAGGT CACTCTGGAA
TATAGACCCG TGATCGACAA AACTTTGAAC GAGGCTGACT GTGCCACCGT CCCGCCAGCC
ATTCGCTCCT ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28693
Clone ID Related Accession (Same CDS sequence) NM_001294332.1
Accession Version NM_001294332.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1851bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Homo sapiens(Human)
Product succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA160919.1, AK295937.1, AK291311.1, AC021087.5 and HY265959.1. Transcript Variant: This variant (2) lacks an in-frame exon in the 5' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK295937.1, SRR1660809.180969.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGTCGGGGG TCCGGGGCCT GTCGCGGCTG CTGAGCGCTC GGCGCCTGGC GCTGGCCAAG 
GCGTGGCCAA CAGTGTTGCA AACAGGAACC CGAGGTTTTC ACTTCACTGT TGATGGGAAC
AAGAGGGCAT CTGCTAAAGT TTCAGATTCC ATTTCTGCTC AGTATCCAGT AGTGGATCAT
GAATTTGATG CAGTGGTGGT AGGCGCTGGA GGGGCAGGCT TGCGAGCTGC ATTTGGCCTT
TCTGAGGCAG GGTTTAATAC AGCATGTGTT ACCAAGCTGT TTCCTACCAG GTCACACACT
GTTGCAGCAC AGCTAGAAAA TTATGGCATG CCGTTTAGCA GAACTGAAGA TGGGAAGATT
TATCAGCGTG CATTTGGTGG ACAGAGCCTC AAGTTTGGAA AGGGCGGGCA GGCCCATCGG
TGCTGCTGTG TGGCTGATCG GACTGGCCAC TCGCTATTGC ACACCTTATA TGGAAGGTCT
CTGCGATATG ATACCAGCTA TTTTGTGGAG TATTTTGCCT TGGATCTCCT GATGGAGAAT
GGGGAGTGCC GTGGTGTCAT CGCACTGTGC ATAGAGGACG GGTCCATCCA TCGCATAAGA
GCAAAGAACA CTGTTGTTGC CACAGGAGGC TACGGGCGCA CCTACTTCAG CTGCACGTCT
GCCCACACCA GCACTGGCGA CGGCACGGCC ATGATCACCA GGGCAGGCCT TCCTTGCCAG
GACCTAGAGT TTGTTCAGTT CCACCCTACA GGCATATATG GTGCTGGTTG TCTCATTACG
GAAGGATGTC GTGGAGAGGG AGGCATTCTC ATTAACAGTC AAGGCGAAAG GTTTATGGAG
CGATACGCCC CTGTCGCGAA GGACCTGGCG TCTAGAGATG TGGTGTCTCG GTCCATGACT
CTGGAGATCC GAGAAGGAAG AGGCTGTGGC CCTGAGAAAG ATCACGTCTA CCTGCAGCTG
CACCACCTAC CTCCAGAGCA GCTGGCCACG CGCCTGCCTG GCATTTCAGA GACAGCCATG
ATCTTCGCTG GCGTGGACGT CACGAAGGAG CCGATCCCTG TCCTCCCCAC CGTGCATTAT
AACATGGGCG GCATTCCCAC CAACTACAAG GGGCAGGTCC TGAGGCACGT GAATGGCCAG
GATCAGATTG TGCCCGGCCT GTACGCCTGT GGGGAGGCCG CCTGTGCCTC GGTACATGGT
GCCAACCGCC TCGGGGCAAA CTCGCTCTTG GACCTGGTTG TCTTTGGTCG GGCATGTGCC
CTGAGCATCG AAGAGTCATG CAGGCCTGGA GATAAAGTCC CTCCAATTAA ACCAAACGCT
GGGGAAGAAT CTGTCATGAA TCTTGACAAA TTGAGATTTG CTGATGGAAG CATAAGAACA
TCGGAACTGC GACTCAGCAT GCAGAAGTCA ATGCAAAATC ATGCTGCCGT GTTCCGTGTG
GGAAGCGTGT TGCAAGAAGG TTGTGGGAAA ATCAGCAAGC TCTATGGAGA CCTAAAGCAC
CTGAAGACGT TCGACCGGGG AATGGTCTGG AACACGGACC TGGTGGAGAC CCTGGAGCTG
CAGAACCTGA TGCTGTGTGC GCTGCAGACC ATCTACGGAG CAGAGGCACG GAAGGAGTCA
CGGGGCGCGC ATGCCAGGGA AGACTACAAG GTGCGGATTG ATGAGTACGA TTACTCCAAG
CCCATCCAGG GGCAACAGAA GAAGCCCTTT GAGGAGCACT GGAGGAAGCA CACCCTGTCC
TATGTGGACG TTGGCACTGG GAAGGTCACT CTGGAATATA GACCCGTGAT CGACAAAACT
TTGAACGAGG CTGACTGTGC CACCGTCCCG CCAGCCATTC GCTCCTACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001281261.1
CDS134..1984
Misc Feature(1)251..1981
Misc Feature(2)<443..1402
Misc Feature(3)1532..1981
Exon (1)1..196
Exon (2)197..283
Exon (3)284..445
Exon (4)446..610
Exon (5)611..759
Exon (6)760..884
Exon (7)885..1053
Exon (8)1054..1249
Exon (9)1250..1421
Exon (10)1422..1540
Exon (11)1541..1652
Exon (12)1653..1783
Exon (13)1784..1897
Exon (14)1898..2646
Translation

Target ORF information:

RefSeq Version NM_001294332.1
Organism Homo sapiens(Human)
Definition Homo sapiens succinate dehydrogenase complex flavoprotein subunit A (SDHA), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001294332.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGTCGGGGG TCCGGGGCCT GTCGCGGCTG CTGAGCGCTC GGCGCCTGGC GCTGGCCAAG 
GCGTGGCCAA CAGTGTTGCA AACAGGAACC CGAGGTTTTC ACTTCACTGT TGATGGGAAC
AAGAGGGCAT CTGCTAAAGT TTCAGATTCC ATTTCTGCTC AGTATCCAGT AGTGGATCAT
GAATTTGATG CAGTGGTGGT AGGCGCTGGA GGGGCAGGCT TGCGAGCTGC ATTTGGCCTT
TCTGAGGCAG GGTTTAATAC AGCATGTGTT ACCAAGCTGT TTCCTACCAG GTCACACACT
GTTGCAGCAC AGCTAGAAAA TTATGGCATG CCGTTTAGCA GAACTGAAGA TGGGAAGATT
TATCAGCGTG CATTTGGTGG ACAGAGCCTC AAGTTTGGAA AGGGCGGGCA GGCCCATCGG
TGCTGCTGTG TGGCTGATCG GACTGGCCAC TCGCTATTGC ACACCTTATA TGGAAGGTCT
CTGCGATATG ATACCAGCTA TTTTGTGGAG TATTTTGCCT TGGATCTCCT GATGGAGAAT
GGGGAGTGCC GTGGTGTCAT CGCACTGTGC ATAGAGGACG GGTCCATCCA TCGCATAAGA
GCAAAGAACA CTGTTGTTGC CACAGGAGGC TACGGGCGCA CCTACTTCAG CTGCACGTCT
GCCCACACCA GCACTGGCGA CGGCACGGCC ATGATCACCA GGGCAGGCCT TCCTTGCCAG
GACCTAGAGT TTGTTCAGTT CCACCCTACA GGCATATATG GTGCTGGTTG TCTCATTACG
GAAGGATGTC GTGGAGAGGG AGGCATTCTC ATTAACAGTC AAGGCGAAAG GTTTATGGAG
CGATACGCCC CTGTCGCGAA GGACCTGGCG TCTAGAGATG TGGTGTCTCG GTCCATGACT
CTGGAGATCC GAGAAGGAAG AGGCTGTGGC CCTGAGAAAG ATCACGTCTA CCTGCAGCTG
CACCACCTAC CTCCAGAGCA GCTGGCCACG CGCCTGCCTG GCATTTCAGA GACAGCCATG
ATCTTCGCTG GCGTGGACGT CACGAAGGAG CCGATCCCTG TCCTCCCCAC CGTGCATTAT
AACATGGGCG GCATTCCCAC CAACTACAAG GGGCAGGTCC TGAGGCACGT GAATGGCCAG
GATCAGATTG TGCCCGGCCT GTACGCCTGT GGGGAGGCCG CCTGTGCCTC GGTACATGGT
GCCAACCGCC TCGGGGCAAA CTCGCTCTTG GACCTGGTTG TCTTTGGTCG GGCATGTGCC
CTGAGCATCG AAGAGTCATG CAGGCCTGGA GATAAAGTCC CTCCAATTAA ACCAAACGCT
GGGGAAGAAT CTGTCATGAA TCTTGACAAA TTGAGATTTG CTGATGGAAG CATAAGAACA
TCGGAACTGC GACTCAGCAT GCAGAAGTCA ATGCAAAATC ATGCTGCCGT GTTCCGTGTG
GGAAGCGTGT TGCAAGAAGG TTGTGGGAAA ATCAGCAAGC TCTATGGAGA CCTAAAGCAC
CTGAAGACGT TCGACCGGGG AATGGTCTGG AACACGGACC TGGTGGAGAC CCTGGAGCTG
CAGAACCTGA TGCTGTGTGC GCTGCAGACC ATCTACGGAG CAGAGGCACG GAAGGAGTCA
CGGGGCGCGC ATGCCAGGGA AGACTACAAG GTGCGGATTG ATGAGTACGA TTACTCCAAG
CCCATCCAGG GGCAACAGAA GAAGCCCTTT GAGGAGCACT GGAGGAAGCA CACCCTGTCC
TATGTGGACG TTGGCACTGG GAAGGTCACT CTGGAATATA GACCCGTGAT CGACAAAACT
TTGAACGAGG CTGACTGTGC CACCGTCCCG CCAGCCATTC GCTCCTACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu39348
Clone ID Related Accession (Same CDS sequence) NM_001330758.1
Accession Version NM_001330758.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1752bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-24
Organism Homo sapiens(Human)
Product succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC021087.5. On Sep 3, 2016 this sequence version replaced XM_005248331.3. Transcript Variant: This variant (3) lacks two in-frame exons in the 3' coding region, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.23296.1, SRR1803616.221477.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGTCGGGGG TCCGGGGCCT GTCGCGGCTG CTGAGCGCTC GGCGCCTGGC GCTGGCCAAG 
GCGTGGCCAA CAGTGTTGCA AACAGGAACC CGAGGTTTTC ACTTCACTGT TGATGGGAAC
AAGAGGGCAT CTGCTAAAGT TTCAGATTCC ATTTCTGCTC AGTATCCAGT AGTGGATCAT
GAATTTGATG CAGTGGTGGT AGGCGCTGGA GGGGCAGGCT TGCGAGCTGC ATTTGGCCTT
TCTGAGGCAG GGTTTAATAC AGCATGTGTT ACCAAGCTGT TTCCTACCAG GTCACACACT
GTTGCAGCAC AGGGAGGAAT CAATGCTGCT CTGGGGAACA TGGAGGAGGA CAACTGGAGG
TGGCATTTCT ACGACACCGT GAAGGGCTCC GACTGGCTGG GGGACCAGGA TGCCATCCAC
TACATGACGG AGCAGGCCCC CGCCGCCGTG GTCGAGCTAG AAAATTATGG CATGCCGTTT
AGCAGAACTG AAGATGGGAA GATTTATCAG CGTGCATTTG GTGGACAGAG CCTCAAGTTT
GGAAAGGGCG GGCAGGCCCA TCGGTGCTGC TGTGTGGCTG ATCGGACTGG CCACTCGCTA
TTGCACACCT TATATGGAAG GTCTCTGCGA TATGATACCA GCTATTTTGT GGAGTATTTT
GCCTTGGATC TCCTGATGGA GAATGGGGAG TGCCGTGGTG TCATCGCACT GTGCATAGAG
GACGGGTCCA TCCATCGCAT AAGAGCAAAG AACACTGTTG TTGCCACAGG AGGCTACGGG
CGCACCTACT TCAGCTGCAC GTCTGCCCAC ACCAGCACTG GCGACGGCAC GGCCATGATC
ACCAGGGCAG GCCTTCCTTG CCAGGACCTA GAGTTTGTTC AGTTCCACCC TACAGGCATA
TATGGTGCTG GTTGTCTCAT TACGGAAGGA TGTCGTGGAG AGGGAGGCAT TCTCATTAAC
AGTCAAGGCG AAAGGTTTAT GGAGCGATAC GCCCCTGTCG CGAAGGACCT GGCGTCTAGA
GATGTGGTGT CTCGGTCCAT GACTCTGGAG ATCCGAGAAG GAAGAGGCTG TGGCCCTGAG
AAAGATCACG TCTACCTGCA GCTGCACCAC CTACCTCCAG AGCAGCTGGC CACGCGCCTG
CCTGGCATTT CAGAGACAGC CATGATCTTC GCTGGCGTGG ACGTCACGAA GGAGCCGATC
CCTGTCCTCC CCACCGTGCA TTATAACATG GGCGGCATTC CCACCAACTA CAAGGGGCAG
GTCCTGAGGC ACGTGAATGG CCAGGATCAG ATTGTGCCCG GCCTGTACGC CTGTGGGGAG
GCCGCCTGTG CCTCGGTACA TGGTGCCAAC CGCCTCGGGG CAAACTCGCT CTTGGACCTG
GTTGTCTTTG GTCGGGCATG TGCCCTGAGC ATCGAAGAGT CATGCAGGCC TGGAGATAAA
GTCCCTCCAA TTAAACCAAA CGCTGGGGAA GAATCTGTCA TGAATCTTGA CAAATTGAGA
TTTGCTGATG GAAGCATAAG AACATCGGAA CTGCGACTCA GCATGCAGAA GGTGCGGATT
GATGAGTACG ATTACTCCAA GCCCATCCAG GGGCAACAGA AGAAGCCCTT TGAGGAGCAC
TGGAGGAAGC ACACCCTGTC CTATGTGGAC GTTGGCACTG GGAAGGTCAC TCTGGAATAT
AGACCCGTGA TCGACAAAAC TTTGAACGAG GCTGACTGTG CCACCGTCCC GCCAGCCATT
CGCTCCTACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001317687.1
CDS134..1885
Misc Feature(1)251..1882
Misc Feature(2)386..1546
Misc Feature(3)428..430
Misc Feature(4)632..634
Misc Feature(5)668..670
Misc Feature(6)668..670
Misc Feature(7)677..679
Misc Feature(8)776..778
Misc Feature(9)881..883
Misc Feature(10)881..883
Misc Feature(11)1136..1138
Misc Feature(12)1136..1138
Misc Feature(13)1571..1573
Misc Feature(14)1586..1588
Misc Feature(15)1586..1588
Misc Feature(16)1625..1627
Misc Feature(17)1625..1627
Misc Feature(18)1682..1684
Misc Feature(19)<1748..1882
Exon (1)1..196
Exon (2)197..283
Exon (3)284..445
Exon (4)446..589
Exon (5)590..754
Exon (6)755..903
Exon (7)904..1028
Exon (8)1029..1197
Exon (9)1198..1393
Exon (10)1394..1565
Exon (11)1566..1684
Exon (12)1685..1798
Exon (13)1799..2547
Translation

Target ORF information:

RefSeq Version NM_001330758.1
Organism Homo sapiens(Human)
Definition Homo sapiens succinate dehydrogenase complex flavoprotein subunit A (SDHA), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001330758.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGTCGGGGG TCCGGGGCCT GTCGCGGCTG CTGAGCGCTC GGCGCCTGGC GCTGGCCAAG 
GCGTGGCCAA CAGTGTTGCA AACAGGAACC CGAGGTTTTC ACTTCACTGT TGATGGGAAC
AAGAGGGCAT CTGCTAAAGT TTCAGATTCC ATTTCTGCTC AGTATCCAGT AGTGGATCAT
GAATTTGATG CAGTGGTGGT AGGCGCTGGA GGGGCAGGCT TGCGAGCTGC ATTTGGCCTT
TCTGAGGCAG GGTTTAATAC AGCATGTGTT ACCAAGCTGT TTCCTACCAG GTCACACACT
GTTGCAGCAC AGGGAGGAAT CAATGCTGCT CTGGGGAACA TGGAGGAGGA CAACTGGAGG
TGGCATTTCT ACGACACCGT GAAGGGCTCC GACTGGCTGG GGGACCAGGA TGCCATCCAC
TACATGACGG AGCAGGCCCC CGCCGCCGTG GTCGAGCTAG AAAATTATGG CATGCCGTTT
AGCAGAACTG AAGATGGGAA GATTTATCAG CGTGCATTTG GTGGACAGAG CCTCAAGTTT
GGAAAGGGCG GGCAGGCCCA TCGGTGCTGC TGTGTGGCTG ATCGGACTGG CCACTCGCTA
TTGCACACCT TATATGGAAG GTCTCTGCGA TATGATACCA GCTATTTTGT GGAGTATTTT
GCCTTGGATC TCCTGATGGA GAATGGGGAG TGCCGTGGTG TCATCGCACT GTGCATAGAG
GACGGGTCCA TCCATCGCAT AAGAGCAAAG AACACTGTTG TTGCCACAGG AGGCTACGGG
CGCACCTACT TCAGCTGCAC GTCTGCCCAC ACCAGCACTG GCGACGGCAC GGCCATGATC
ACCAGGGCAG GCCTTCCTTG CCAGGACCTA GAGTTTGTTC AGTTCCACCC TACAGGCATA
TATGGTGCTG GTTGTCTCAT TACGGAAGGA TGTCGTGGAG AGGGAGGCAT TCTCATTAAC
AGTCAAGGCG AAAGGTTTAT GGAGCGATAC GCCCCTGTCG CGAAGGACCT GGCGTCTAGA
GATGTGGTGT CTCGGTCCAT GACTCTGGAG ATCCGAGAAG GAAGAGGCTG TGGCCCTGAG
AAAGATCACG TCTACCTGCA GCTGCACCAC CTACCTCCAG AGCAGCTGGC CACGCGCCTG
CCTGGCATTT CAGAGACAGC CATGATCTTC GCTGGCGTGG ACGTCACGAA GGAGCCGATC
CCTGTCCTCC CCACCGTGCA TTATAACATG GGCGGCATTC CCACCAACTA CAAGGGGCAG
GTCCTGAGGC ACGTGAATGG CCAGGATCAG ATTGTGCCCG GCCTGTACGC CTGTGGGGAG
GCCGCCTGTG CCTCGGTACA TGGTGCCAAC CGCCTCGGGG CAAACTCGCT CTTGGACCTG
GTTGTCTTTG GTCGGGCATG TGCCCTGAGC ATCGAAGAGT CATGCAGGCC TGGAGATAAA
GTCCCTCCAA TTAAACCAAA CGCTGGGGAA GAATCTGTCA TGAATCTTGA CAAATTGAGA
TTTGCTGATG GAAGCATAAG AACATCGGAA CTGCGACTCA GCATGCAGAA GGTGCGGATT
GATGAGTACG ATTACTCCAA GCCCATCCAG GGGCAACAGA AGAAGCCCTT TGAGGAGCAC
TGGAGGAAGC ACACCCTGTC CTATGTGGAC GTTGGCACTG GGAAGGTCAC TCTGGAATAT
AGACCCGTGA TCGACAAAAC TTTGAACGAG GCTGACTGTG CCACCGTCCC GCCAGCCATT
CGCTCCTACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60441
Clone ID Related Accession (Same CDS sequence) XM_011514073.1
Accession Version XM_011514073.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1800bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGTCGGGGG TCCGGGGCCT GTCGCGGCTG CTGAGCGCTC GGCGCCTGGC GCTGGCCAAG 
GCGTGGCCAA CAGTGTTGCA AACAGGAACC CGAGGTTTTC ACTTCACTGT TGATGGGAAC
AAGAGGGCAT CTGCTAAAGT TTCAGATTCC ATTTCTGCTC AGTATCCAGT AGTGGATCAT
GAATTTGATG CAGTGGTGGT AGGCGCTGGA GGGGCAGGCT TGCGAGCTGC ATTTGGCCTT
TCTGAGGCAG GGTTTAATAC AGCATGTGTT ACCAAGCTGT TTCCTACCAG GTCACACACT
GTTGCAGCAC AGGGAGGAAT CAATGCTGCT CTGGGGAACA TGGAGGAGGA CAACTGGAGG
TGGCATTTCT ACGACACCGT GAAGGGCTCC GACTGGCTGG GGGACCAGGA TGCCATCCAC
TACATGACGG AGCAGGCCCC CGCCGCCGTG GTCGAGCTAG AAAATTATGG CATGCCGTTT
AGCAGAACTG AAGATGGGAA GATTTATCAG CGTGCATTTG GTGGACAGAG CCTCAAGTTT
GGAAAGGGCG GGCAGGCCCA TCGGTGCTGC TGTGTGGCTG ATCGGACTGG CCACTCGCTA
TTGCACACCT TATATGGAAG GTCTCTGCGA TATGATACCA GCTATTTTGT GGAGTATTTT
GCCTTGGATC TCCTGATGGA GAATGGGGAG TGCCGTGGTG TCATCGCACT GTGCATAGAG
GACGGGTCCA TCCATCGCAT AAGAGCAAAG AACACTGTTG TTGCCACAGG AGGCTACGGG
CGCACCTACT TCAGCTGCAC GTCTGCCCAC ACCAGCACTG GCGACGGCAC GGCCATGATC
ACCAGGGCAG GCCTTCCTTG CCAGGACCTA GAGTTTGTTC AGTTCCACCC TACAGGCATA
TATGGTGCTG GTTGTCTCAT TACGGAAGGA TGTCGTGGAG AGGGAGGCAT TCTCATTAAC
AGTCAAGGCG AAAGGTTTAT GGAGCGATAC GCCCCTGTCG CGAAGGACCT GGCGTCTAGA
GATGTGGTGT CTCGGTCCAT GACTCTGGAG ATCCGAGAAG GAAGAGGCTG TGGCCCTGAG
AAAGATCACG TCTACCTGCA GCTGCACCAC CTACCTCCAG AGCAGCTGGC CACGCGCCTG
CCTGGCATTT CAGAGACAGC CATGATCTTC GCTGGCGTGG ACGTCACGAA GGAGCCGATC
CCTGTCCTCC CCACCGTGCA TTATAACATG GGCGGCATTC CCACCAACTA CAAGGGGCAG
GTCCTGAGGC ACGTGAATGG CCAGGATCAG ATTGTGCCCG GCCTGTACGC CTGTGGGGAG
GCCGCCTGTG CCTCGGTACA TGGTGCCAAC CGCCTCGGGG CAAACTCGCT CTTGGACCTG
GTTGTCTTTG GTCGGGCATG TGCCCTGAGC ATCGAAGAGT CATGCAGGCC TGGAGATAAA
GTCCCTCCAA TTAAACCAAA CGCTGGGGAA GAATCTGTCA TGAATCTTGA CAAATTGAGA
TTTGCTGATG GAAGCATAAG AACATCGGAA CTGCGACTCA GCATGCAGAA GGTGCGGATT
GATGAGTACG ATTACTCCAA GCCCATCCAG GGGCAACAGA AGAAGCCCTT TGAGGAGCAC
TGGAGGAAGC ACACCCTGTC CTATGTGGAC GTTGGCACTG GGAAGTGCAC AGCCAGAGAA
CAACACATCA CACACAGGAA ACAGTTGTGC TCATGTGATG GGGGCCTCAG CACTAGGAAG
GAGCGGACTA TTGGTGCACG CAGCAGCTTG AATAAATCTG AAAGTCACTA TGCTGTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011512375.1
CDS134..1933
Misc Feature(1)371..1798
Misc Feature(2)386..1546
Translation

Target ORF information:

RefSeq Version XM_011514073.1
Organism Homo sapiens(Human)
Definition Homo sapiens succinate dehydrogenase complex flavoprotein subunit A (SDHA), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011514073.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGTCGGGGG TCCGGGGCCT GTCGCGGCTG CTGAGCGCTC GGCGCCTGGC GCTGGCCAAG 
GCGTGGCCAA CAGTGTTGCA AACAGGAACC CGAGGTTTTC ACTTCACTGT TGATGGGAAC
AAGAGGGCAT CTGCTAAAGT TTCAGATTCC ATTTCTGCTC AGTATCCAGT AGTGGATCAT
GAATTTGATG CAGTGGTGGT AGGCGCTGGA GGGGCAGGCT TGCGAGCTGC ATTTGGCCTT
TCTGAGGCAG GGTTTAATAC AGCATGTGTT ACCAAGCTGT TTCCTACCAG GTCACACACT
GTTGCAGCAC AGGGAGGAAT CAATGCTGCT CTGGGGAACA TGGAGGAGGA CAACTGGAGG
TGGCATTTCT ACGACACCGT GAAGGGCTCC GACTGGCTGG GGGACCAGGA TGCCATCCAC
TACATGACGG AGCAGGCCCC CGCCGCCGTG GTCGAGCTAG AAAATTATGG CATGCCGTTT
AGCAGAACTG AAGATGGGAA GATTTATCAG CGTGCATTTG GTGGACAGAG CCTCAAGTTT
GGAAAGGGCG GGCAGGCCCA TCGGTGCTGC TGTGTGGCTG ATCGGACTGG CCACTCGCTA
TTGCACACCT TATATGGAAG GTCTCTGCGA TATGATACCA GCTATTTTGT GGAGTATTTT
GCCTTGGATC TCCTGATGGA GAATGGGGAG TGCCGTGGTG TCATCGCACT GTGCATAGAG
GACGGGTCCA TCCATCGCAT AAGAGCAAAG AACACTGTTG TTGCCACAGG AGGCTACGGG
CGCACCTACT TCAGCTGCAC GTCTGCCCAC ACCAGCACTG GCGACGGCAC GGCCATGATC
ACCAGGGCAG GCCTTCCTTG CCAGGACCTA GAGTTTGTTC AGTTCCACCC TACAGGCATA
TATGGTGCTG GTTGTCTCAT TACGGAAGGA TGTCGTGGAG AGGGAGGCAT TCTCATTAAC
AGTCAAGGCG AAAGGTTTAT GGAGCGATAC GCCCCTGTCG CGAAGGACCT GGCGTCTAGA
GATGTGGTGT CTCGGTCCAT GACTCTGGAG ATCCGAGAAG GAAGAGGCTG TGGCCCTGAG
AAAGATCACG TCTACCTGCA GCTGCACCAC CTACCTCCAG AGCAGCTGGC CACGCGCCTG
CCTGGCATTT CAGAGACAGC CATGATCTTC GCTGGCGTGG ACGTCACGAA GGAGCCGATC
CCTGTCCTCC CCACCGTGCA TTATAACATG GGCGGCATTC CCACCAACTA CAAGGGGCAG
GTCCTGAGGC ACGTGAATGG CCAGGATCAG ATTGTGCCCG GCCTGTACGC CTGTGGGGAG
GCCGCCTGTG CCTCGGTACA TGGTGCCAAC CGCCTCGGGG CAAACTCGCT CTTGGACCTG
GTTGTCTTTG GTCGGGCATG TGCCCTGAGC ATCGAAGAGT CATGCAGGCC TGGAGATAAA
GTCCCTCCAA TTAAACCAAA CGCTGGGGAA GAATCTGTCA TGAATCTTGA CAAATTGAGA
TTTGCTGATG GAAGCATAAG AACATCGGAA CTGCGACTCA GCATGCAGAA GGTGCGGATT
GATGAGTACG ATTACTCCAA GCCCATCCAG GGGCAACAGA AGAAGCCCTT TGAGGAGCAC
TGGAGGAAGC ACACCCTGTC CTATGTGGAC GTTGGCACTG GGAAGTGCAC AGCCAGAGAA
CAACACATCA CACACAGGAA ACAGTTGTGC TCATGTGATG GGGGCCTCAG CACTAGGAAG
GAGCGGACTA TTGGTGCACG CAGCAGCTTG AATAAATCTG AAAGTCACTA TGCTGTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60440
Clone ID Related Accession (Same CDS sequence) XM_011514072.1
Accession Version XM_011514072.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2043bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGTCGGGGG TCCGGGGCCT GTCGCGGCTG CTGAGCGCTC GGCGCCTGGC GCTGGCCAAG 
GCGTGGCCAA CAGTGTTGCA AACAGGAACC CGAGGTTTTC ACTTCACTGT TGATGGGAAC
AAGAGGGCAT CTGCTAAAGT TTCAGATTCC ATTTCTGCTC AGTATCCAGT AGTGGATCAT
GAATTTGATG CAGTGGTGGT AGGCGCTGGA GGGGCAGGCT TGCGAGCTGC ATTTGGCCTT
TCTGAGGCAG GGTTTAATAC AGCATGTGTT ACCAAGCTGT TTCCTACCAG GTCACACACT
GTTGCAGCAC AGGGAGGAAT CAATGCTGCT CTGGGGAACA TGGAGGAGGA CAACTGGAGG
TGGCATTTCT ACGACACCGT GAAGGGCTCC GACTGGCTGG GGGACCAGGA TGCCATCCAC
TACATGACGG AGCAGGCCCC CGCCGCCGTG GTCGAGCTAG AAAATTATGG CATGCCGTTT
AGCAGAACTG AAGATGGGAA GATTTATCAG CGTGCATTTG GTGGACAGAG CCTCAAGTTT
GGAAAGGGCG GGCAGGCCCA TCGGTGCTGC TGTGTGGCTG ATCGGACTGG CCACTCGCTA
TTGCACACCT TATATGGAAG GTCTCTGCGA TATGATACCA GCTATTTTGT GGAGTATTTT
GCCTTGGATC TCCTGATGGA GAATGGGGAG TGCCGTGGTG TCATCGCACT GTGCATAGAG
GACGGGTCCA TCCATCGCAT AAGAGCAAAG AACACTGTTG TTGCCACAGG AGGCTACGGG
CGCACCTACT TCAGCTGCAC GTCTGCCCAC ACCAGCACTG GCGACGGCAC GGCCATGATC
ACCAGGGCAG GCCTTCCTTG CCAGGACCTA GAGTTTGTTC AGTTCCACCC TACAGGCATA
TATGGTGCTG GTTGTCTCAT TACGGAAGGA TGTCGTGGAG AGGGAGGCAT TCTCATTAAC
AGTCAAGGCG AAAGGTTTAT GGAGCGATAC GCCCCTGTCG CGAAGGACCT GGCGTCTAGA
GATGTGGTGT CTCGGTCCAT GACTCTGGAG ATCCGAGAAG GAAGAGGCTG TGGCCCTGAG
AAAGATCACG TCTACCTGCA GCTGCACCAC CTACCTCCAG AGCAGCTGGC CACGCGCCTG
CCTGGCATTT CAGAGACAGC CATGATCTTC GCTGGCGTGG ACGTCACGAA GGAGCCGATC
CCTGTCCTCC CCACCGTGCA TTATAACATG GGCGGCATTC CCACCAACTA CAAGGGGCAG
GTCCTGAGGC ACGTGAATGG CCAGGATCAG ATTGTGCCCG GCCTGTACGC CTGTGGGGAG
GCCGCCTGTG CCTCGGTACA TGGTGCCAAC CGCCTCGGGG CAAACTCGCT CTTGGACCTG
GTTGTCTTTG GTCGGGCATG TGCCCTGAGC ATCGAAGAGT CATGCAGGCC TGGAGATAAA
GTCCCTCCAA TTAAACCAAA CGCTGGGGAA GAATCTGTCA TGAATCTTGA CAAATTGAGA
TTTGCTGATG GAAGCATAAG AACATCGGAA CTGCGACTCA GCATGCAGAA GTCAATGCAA
AATCATGCTG CCGTGTTCCG TGTGGGAAGC GTGTTGCAAG AAGGTTGTGG GAAAATCAGC
AAGCTCTATG GAGACCTAAA GCACCTGAAG ACGTTCGACC GGGGAATGGT CTGGAACACG
GACCTGGTGG AGACCCTGGA GCTGCAGAAC CTGATGCTGT GTGCGCTGCA GACCATCTAC
GGAGCAGAGG CACGGAAGGA GTCACGGGGC GCGCATGCCA GGGAAGACTA CAAGGTGCGG
ATTGATGAGT ACGATTACTC CAAGCCCATC CAGGGGCAAC AGAAGAAGCC CTTTGAGGAG
CACTGGAGGA AGCACACCCT GTCCTATGTG GACGTTGGCA CTGGGAAGTG CACAGCCAGA
GAACAACACA TCACACACAG GAAACAGTTG TGCTCATGTG ATGGGGGCCT CAGCACTAGG
AAGGAGCGGA CTATTGGTGC ACGCAGCAGC TTGAATAAAT CTGAAAGTCA CTATGCTGTG
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011512374.1
CDS134..2176
Misc Feature(1)251..2041
Misc Feature(2)386..1546
Misc Feature(3)1676..2038
Translation

Target ORF information:

RefSeq Version XM_011514072.1
Organism Homo sapiens(Human)
Definition Homo sapiens succinate dehydrogenase complex flavoprotein subunit A (SDHA), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011514072.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGTCGGGGG TCCGGGGCCT GTCGCGGCTG CTGAGCGCTC GGCGCCTGGC GCTGGCCAAG 
GCGTGGCCAA CAGTGTTGCA AACAGGAACC CGAGGTTTTC ACTTCACTGT TGATGGGAAC
AAGAGGGCAT CTGCTAAAGT TTCAGATTCC ATTTCTGCTC AGTATCCAGT AGTGGATCAT
GAATTTGATG CAGTGGTGGT AGGCGCTGGA GGGGCAGGCT TGCGAGCTGC ATTTGGCCTT
TCTGAGGCAG GGTTTAATAC AGCATGTGTT ACCAAGCTGT TTCCTACCAG GTCACACACT
GTTGCAGCAC AGGGAGGAAT CAATGCTGCT CTGGGGAACA TGGAGGAGGA CAACTGGAGG
TGGCATTTCT ACGACACCGT GAAGGGCTCC GACTGGCTGG GGGACCAGGA TGCCATCCAC
TACATGACGG AGCAGGCCCC CGCCGCCGTG GTCGAGCTAG AAAATTATGG CATGCCGTTT
AGCAGAACTG AAGATGGGAA GATTTATCAG CGTGCATTTG GTGGACAGAG CCTCAAGTTT
GGAAAGGGCG GGCAGGCCCA TCGGTGCTGC TGTGTGGCTG ATCGGACTGG CCACTCGCTA
TTGCACACCT TATATGGAAG GTCTCTGCGA TATGATACCA GCTATTTTGT GGAGTATTTT
GCCTTGGATC TCCTGATGGA GAATGGGGAG TGCCGTGGTG TCATCGCACT GTGCATAGAG
GACGGGTCCA TCCATCGCAT AAGAGCAAAG AACACTGTTG TTGCCACAGG AGGCTACGGG
CGCACCTACT TCAGCTGCAC GTCTGCCCAC ACCAGCACTG GCGACGGCAC GGCCATGATC
ACCAGGGCAG GCCTTCCTTG CCAGGACCTA GAGTTTGTTC AGTTCCACCC TACAGGCATA
TATGGTGCTG GTTGTCTCAT TACGGAAGGA TGTCGTGGAG AGGGAGGCAT TCTCATTAAC
AGTCAAGGCG AAAGGTTTAT GGAGCGATAC GCCCCTGTCG CGAAGGACCT GGCGTCTAGA
GATGTGGTGT CTCGGTCCAT GACTCTGGAG ATCCGAGAAG GAAGAGGCTG TGGCCCTGAG
AAAGATCACG TCTACCTGCA GCTGCACCAC CTACCTCCAG AGCAGCTGGC CACGCGCCTG
CCTGGCATTT CAGAGACAGC CATGATCTTC GCTGGCGTGG ACGTCACGAA GGAGCCGATC
CCTGTCCTCC CCACCGTGCA TTATAACATG GGCGGCATTC CCACCAACTA CAAGGGGCAG
GTCCTGAGGC ACGTGAATGG CCAGGATCAG ATTGTGCCCG GCCTGTACGC CTGTGGGGAG
GCCGCCTGTG CCTCGGTACA TGGTGCCAAC CGCCTCGGGG CAAACTCGCT CTTGGACCTG
GTTGTCTTTG GTCGGGCATG TGCCCTGAGC ATCGAAGAGT CATGCAGGCC TGGAGATAAA
GTCCCTCCAA TTAAACCAAA CGCTGGGGAA GAATCTGTCA TGAATCTTGA CAAATTGAGA
TTTGCTGATG GAAGCATAAG AACATCGGAA CTGCGACTCA GCATGCAGAA GTCAATGCAA
AATCATGCTG CCGTGTTCCG TGTGGGAAGC GTGTTGCAAG AAGGTTGTGG GAAAATCAGC
AAGCTCTATG GAGACCTAAA GCACCTGAAG ACGTTCGACC GGGGAATGGT CTGGAACACG
GACCTGGTGG AGACCCTGGA GCTGCAGAAC CTGATGCTGT GTGCGCTGCA GACCATCTAC
GGAGCAGAGG CACGGAAGGA GTCACGGGGC GCGCATGCCA GGGAAGACTA CAAGGTGCGG
ATTGATGAGT ACGATTACTC CAAGCCCATC CAGGGGCAAC AGAAGAAGCC CTTTGAGGAG
CACTGGAGGA AGCACACCCT GTCCTATGTG GACGTTGGCA CTGGGAAGTG CACAGCCAGA
GAACAACACA TCACACACAG GAAACAGTTG TGCTCATGTG ATGGGGGCCT CAGCACTAGG
AAGGAGCGGA CTATTGGTGC ACGCAGCAGC TTGAATAAAT CTGAAAGTCA CTATGCTGTG
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu95977
Clone ID Related Accession (Same CDS sequence) XM_017009685.1
Accession Version XM_017009685.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2358bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGTCGGGGG TCCGGGGCCT GTCGCGGCTG CTGAGCGCTC GGCGCCTGGC GCTGGCCAAG 
GCGTGGCCAA CAGTGTTGCA AACAGGAACC CGAGGTTTTC ACTTCACTGT TGATGGGAAC
AAGAGGGCAT CTGCTAAAGT TTCAGATTCC ATTTCTGCTC AGTATCCAGT AGTGGATCAT
GAATTTGATG CAGTGGTGGT AGGCGCTGGA GGGGCAGGCT TGCGAGCTGC ATTTGGCCTT
TCTGAGGCAG GGTTTAATAC AGCATGTGTT ACCAAGCTGT TTCCTACCAG GTCACACACT
GTTGCAGCAC AGGGAGGAAT CAATGCTGCT CTGGGGAACA TGGAGGAGGA CAACTGGAGG
TGGCATTTCT ACGACACCGT GAAGGGCTCC GACTGGCTGG GGGACCAGGA TGCCATCCAC
TACATGACGG AGCAGGCCCC CGCCGCCGTG GTCGAGCTAG AAAATTATGG CATGCCGTTT
AGCAGAACTG AAGATGGGAA GATTTATCAG CGTGCATTTG GTGGACAGAG CCTCAAGTTT
GGAAAGGGCG GGCAGGCCCA TCGGTGCTGC TGTGTGGCTG ATCGGACTGG CCACTCGCTA
TTGCACACCT TATATGGAAG GTCTCTGCGA TATGATACCA GCTATTTTGT GGAGTATTTT
GCCTTGGATC TCCTGATGGA GAATGGGGAG TGCCGTGGTG TCATCGCACT GTGCATAGAG
GACGGGTCCA TCCATCGCAT AAGAGCAAAG AACACTGTTG TTGCCACAGG AGGCTACGGG
CGCACCTACT TCAGCTGCAC GTCTGCCCAC ACCAGCACTG GCGACGGCAC GGCCATGATC
ACCAGGGCAG GCCTTCCTTG CCAGGACCTA GAGTTTGTTC AGTTCCACCC TACAGGCATA
TATGGTGCTG GTTGTCTCAT TACGGAAGGA TGTCGTGGAG AGGGAGGCAT TCTCATTAAC
AGTCAAGGCG AAAGGTTTAT GGAGCGATAC GCCCCTGTCG CGAAGGACCT GGCGTCTAGA
GATGTGGTGT CTCGGTCCAT GACTCTGGAG ATCCGAGAAG GAAGAGGCTG TGGCCCTGAG
AAAGATCACG TCTACCTGCA GCTGCACCAC CTACCTCCAG AGCAGCTGGC CACGCGCCTG
CCTGGCATTT CAGAGACAGC CATGATCTTC GCTGGCGTGG ACGTCACGAA GGAGCCGATC
CCTGTCCTCC CCACCGTGCA TTATAACATG GGCGGCATTC CCACCAACTA CAAGGGGCAG
GTCCTGAGGC ACGTGAATGG CCAGGATCAG ATTGTGCCCG GCCTGTACGC CTGTGGGGAG
GCCGCCTGTG CCTCGGTACA TGGTGCCAAC CGCCTCGGGG CAAACTCGCT CTTGGACCTG
GTTGTCTTTG GTCGGGCATG TGCCCTGAGC ATCGAAGAGT CATGCAGGCC TGGAGATAAA
GTCCCTCCAA TTAAACCAAA CGCTGGGGAA GAATCTGTCA TGAATCTTGA CAAATTGAGA
TTTGCTGATG GAAGCATAAG AACATCGGAA CTGCGACTCA GCATGCAGAA GTCAATGCAA
AATCATGCTG CCGTGTTCCG TGTGGGAAGC GTGTTGCAAG AAGGTTGTGG GAAAATCAGC
AAGCTCTATG GAGACCTAAA GCACCTGAAG ACGTTCGACC GGGGAATGGT CTGGAACACG
GACCTGGTGG AGACCCTGGA GCTGCAGAAC CTGATGCTGT GTGCGCTGCA GACCATCTAC
GGAGCAGAGG CACGGAAGGA GTCACGGGGC GCGCATGCCA GGGAAGACTA CAAGGTGCGG
ATTGATGAGT ACGATTACTC CAAGCCCATC CAGGGGCAAC AGAAGAAGCC CTTTGAGGAG
CACTGGAGGA AGCACACCCT GTCCTATGTG GACGTTGGCA CTGGGAAGGT CAGTGTGGAG
CTCGTTCTCA CCACAGCCCA GCACCCACAC GGCCCCGCCC AGGCCTGCGG GCTGGCCTTG
CTGATGGTGA ACGGGGAAGA GCAGGCCAGA TTTAAATCAA CTCCCGACAG ATTCGAGGCA
CCGCTGAAAA AGGCACTCCG ACAGCAGTCG GGCTTCGGGC TGGAAACAGA ATCCAGTTCC
TGCAGGTGGT TCAGAGGAGC CTTAAGGAAG GGTTGCTCCG TGGTGTGCGC CAGATGGACG
TCACTGGGCA GGAGCAAGTG TCCAAGGCCT GGTGGTAGGG GAGGAGATGA TGATTGTGGA
CCTAGCGAGA AAGCATCTGT GGGGTGGGGA CACACAGCCA TTACCAGAAA CCAGTCCCAC
GTTAAGGGAG CCCAGAAGAG ACACCTCCCC TCTCCTCCCA CGGGCTGGGC CAACTGGAAG
CGTCTGCAGG GGAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016865174.1
CDS134..2491
Translation

Target ORF information:

RefSeq Version XM_017009685.1
Organism Homo sapiens(Human)
Definition Homo sapiens succinate dehydrogenase complex flavoprotein subunit A (SDHA), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017009685.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGTCGGGGG TCCGGGGCCT GTCGCGGCTG CTGAGCGCTC GGCGCCTGGC GCTGGCCAAG 
GCGTGGCCAA CAGTGTTGCA AACAGGAACC CGAGGTTTTC ACTTCACTGT TGATGGGAAC
AAGAGGGCAT CTGCTAAAGT TTCAGATTCC ATTTCTGCTC AGTATCCAGT AGTGGATCAT
GAATTTGATG CAGTGGTGGT AGGCGCTGGA GGGGCAGGCT TGCGAGCTGC ATTTGGCCTT
TCTGAGGCAG GGTTTAATAC AGCATGTGTT ACCAAGCTGT TTCCTACCAG GTCACACACT
GTTGCAGCAC AGGGAGGAAT CAATGCTGCT CTGGGGAACA TGGAGGAGGA CAACTGGAGG
TGGCATTTCT ACGACACCGT GAAGGGCTCC GACTGGCTGG GGGACCAGGA TGCCATCCAC
TACATGACGG AGCAGGCCCC CGCCGCCGTG GTCGAGCTAG AAAATTATGG CATGCCGTTT
AGCAGAACTG AAGATGGGAA GATTTATCAG CGTGCATTTG GTGGACAGAG CCTCAAGTTT
GGAAAGGGCG GGCAGGCCCA TCGGTGCTGC TGTGTGGCTG ATCGGACTGG CCACTCGCTA
TTGCACACCT TATATGGAAG GTCTCTGCGA TATGATACCA GCTATTTTGT GGAGTATTTT
GCCTTGGATC TCCTGATGGA GAATGGGGAG TGCCGTGGTG TCATCGCACT GTGCATAGAG
GACGGGTCCA TCCATCGCAT AAGAGCAAAG AACACTGTTG TTGCCACAGG AGGCTACGGG
CGCACCTACT TCAGCTGCAC GTCTGCCCAC ACCAGCACTG GCGACGGCAC GGCCATGATC
ACCAGGGCAG GCCTTCCTTG CCAGGACCTA GAGTTTGTTC AGTTCCACCC TACAGGCATA
TATGGTGCTG GTTGTCTCAT TACGGAAGGA TGTCGTGGAG AGGGAGGCAT TCTCATTAAC
AGTCAAGGCG AAAGGTTTAT GGAGCGATAC GCCCCTGTCG CGAAGGACCT GGCGTCTAGA
GATGTGGTGT CTCGGTCCAT GACTCTGGAG ATCCGAGAAG GAAGAGGCTG TGGCCCTGAG
AAAGATCACG TCTACCTGCA GCTGCACCAC CTACCTCCAG AGCAGCTGGC CACGCGCCTG
CCTGGCATTT CAGAGACAGC CATGATCTTC GCTGGCGTGG ACGTCACGAA GGAGCCGATC
CCTGTCCTCC CCACCGTGCA TTATAACATG GGCGGCATTC CCACCAACTA CAAGGGGCAG
GTCCTGAGGC ACGTGAATGG CCAGGATCAG ATTGTGCCCG GCCTGTACGC CTGTGGGGAG
GCCGCCTGTG CCTCGGTACA TGGTGCCAAC CGCCTCGGGG CAAACTCGCT CTTGGACCTG
GTTGTCTTTG GTCGGGCATG TGCCCTGAGC ATCGAAGAGT CATGCAGGCC TGGAGATAAA
GTCCCTCCAA TTAAACCAAA CGCTGGGGAA GAATCTGTCA TGAATCTTGA CAAATTGAGA
TTTGCTGATG GAAGCATAAG AACATCGGAA CTGCGACTCA GCATGCAGAA GTCAATGCAA
AATCATGCTG CCGTGTTCCG TGTGGGAAGC GTGTTGCAAG AAGGTTGTGG GAAAATCAGC
AAGCTCTATG GAGACCTAAA GCACCTGAAG ACGTTCGACC GGGGAATGGT CTGGAACACG
GACCTGGTGG AGACCCTGGA GCTGCAGAAC CTGATGCTGT GTGCGCTGCA GACCATCTAC
GGAGCAGAGG CACGGAAGGA GTCACGGGGC GCGCATGCCA GGGAAGACTA CAAGGTGCGG
ATTGATGAGT ACGATTACTC CAAGCCCATC CAGGGGCAAC AGAAGAAGCC CTTTGAGGAG
CACTGGAGGA AGCACACCCT GTCCTATGTG GACGTTGGCA CTGGGAAGGT CAGTGTGGAG
CTCGTTCTCA CCACAGCCCA GCACCCACAC GGCCCCGCCC AGGCCTGCGG GCTGGCCTTG
CTGATGGTGA ACGGGGAAGA GCAGGCCAGA TTTAAATCAA CTCCCGACAG ATTCGAGGCA
CCGCTGAAAA AGGCACTCCG ACAGCAGTCG GGCTTCGGGC TGGAAACAGA ATCCAGTTCC
TGCAGGTGGT TCAGAGGAGC CTTAAGGAAG GGTTGCTCCG TGGTGTGCGC CAGATGGACG
TCACTGGGCA GGAGCAAGTG TCCAAGGCCT GGTGGTAGGG GAGGAGATGA TGATTGTGGA
CCTAGCGAGA AAGCATCTGT GGGGTGGGGA CACACAGCCA TTACCAGAAA CCAGTCCCAC
GTTAAGGGAG CCCAGAAGAG ACACCTCCCC TCTCCTCCCA CGGGCTGGGC CAACTGGAAG
CGTCTGCAGG GGAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Cancer-associated fibroblasts enhance pancreatic cancer cell invasion by remodeling the metabolic conversion mechanism.
Oncology reports37(4)1971-1979(2017 Apr)
Shan T,Chen S,Chen X,Lin WR,Li W,Ma J,Wu T,Cui X,Ji H,Li Y,Kang Y


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Potential Pitfalls of SDH Immunohistochemical Detection in Paragangliomas and Phaeochromocytomas Harbouring Germline
Anticancer research37(2)805-812(2017 02)
Santi R,Rapizzi E,Canu L,Ercolino T,Baroni G,Fucci R,Costa G,Mannelli M,Nesi G


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Inhibition of succinate dehydrogenase sensitizes cyclin E-driven ovarian cancer to CDK inhibition.
BioFactors (Oxford, England)42(2)171-8(2016 Mar-Apr)
Guo T,Gu C,Chen X,Kang Y,Li B,Xu C


book

Cardiac-specific succinate dehydrogenase deficiency in Barth syndrome.
EMBO molecular medicine8(2)139-54(2016 Feb)
Dudek J,Cheng IF,Chowdhury A,Wozny K,Balleininger M,Reinhold R,Grunau S,Callegari S,Toischer K,Wanders RJ,Hasenfuß G,Brügger B,Guan K,Rehling P


 
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