UBE2O cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Gene Symbol	UBE2O
Entrez Gene ID	63893
Full Name	ubiquitin conjugating enzyme E2 O
Synonyms	E2-230K
General protein information	Preferred Names ubiquitin conjugating enzyme E2 O Names (E3-independent) E2 ubiquitin-conjugating enzyme E2/E3 hybrid ubiquitin-protein ligase UBE2O ubiquitin carrier protein O ubiquitin conjugating enzyme E2O ubiquitin-conjugating enzyme E2 of 230 kDa ubiquitin-conjugating enzyme E2-230K ubiquitin-protein ligase O
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q25.1
Disorder MIM:	617649

mRNA and Protein(s)

mRNA	Protein	Name
XM_005257567.1	XP_005257624.1	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X4
XM_024450866.1	XP_024306634.1	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X2
NM_022066.3	NP_071349.3	(E3-independent) E2 ubiquitin-conjugating enzyme
XM_024450865.1	XP_024306633.1	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X1
XM_017024935.1	XP_016880424.1	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X5
XM_017024934.1	XP_016880423.1	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X2
NM_022066.4	NP_071349.3	(E3-independent) E2 ubiquitin-conjugating enzyme
XM_024450867.1	XP_024306635.1	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	UBE2O	XP_001139963.2
Bos taurus (cattle)	UBE2O	XP_002696213.1
Gallus gallus (chicken)	UBE2O	XP_415619.4
Homo sapiens (human)	UBE2O	NP_071349.3
Rattus norvegicus (Norway rat)	Ube2o	XP_221132.5
	UBE2O	XP_001099166.2
Canis lupus familiaris (dog)	UBE2O	XP_540449.3
Mus musculus (house mouse)	Ube2o	NP_776116.2
Xenopus tropicalis (tropical clawed frog)	ube2o	XP_002936000.2

Gene Ontology
Bibliography

Related articles in PubMed

UBE2O is a quality control factor for orphans of multiprotein complexes.
Yanagitani K, Juszkiewicz S, Hegde RS
Science (New York, N.Y.)357(6350)472-475(2017 08)

The ubiquitin-conjugating enzyme UBE2O modulates c-Maf stability and induces myeloma cell apoptosis.
Xu Y, Zhang Z, Li J, Tong J, Cao B, Taylor P, Tang X, Wu D, Moran MF, Zeng Y, Mao X
Journal of hematology & oncology10(1)132(2017 Jul)

A UBE2O-AMPK?2 Axis that Promotes Tumor Initiation and Progression Offers Opportunities for Therapy.
Vila IK, Yao Y, Kim G, Xia W, Kim H, Kim SJ, Park MK, Hwang JP, Gonz?lez-Billalabeitia E, Hung MC, Song SJ, Song MS
Cancer cell31(2)208-224(2017 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

UBE2O is a self-contained quality control factor that comprises substrate recognition and ubiquitin transfer activities within a single protein to efficiently target orphans of multiprotein complexes for degradation.
Title: UBE2O is a quality control factor for orphans of multiprotein complexes.

UBE2O mediates c-Maf polyubiquitination and degradation, induces MM cell apoptosis, and suppresses myeloma tumor growth, which provides a novel insight in understanding myelomagenesis and UBE2O biology.
Title: The ubiquitin-conjugating enzyme UBE2O modulates c-Maf stability and induces myeloma cell apoptosis.

UBE2O specifically targets AMPKalpha2 for ubiquitination and degradation, and thereby promotes activation of the mTOR-HIF1alpha pathway.
Title: A UBE2O-AMPK?2 Axis that Promotes Tumor Initiation and Progression Offers Opportunities for Therapy.

UBE2O defines an atypical ubiquitin-signaling pathway that coordinates the function of BAP1.
Title: Autodeubiquitination protects the tumor suppressor BAP1 from cytoplasmic sequestration mediated by the atypical ubiquitin ligase UBE2O.

monoubiquitinated SMAD6 impairs the binding affinity of non-modified SMAD6 to the BMP type I receptor. Moreover, UBE2O and SMAD6 cooperated in the regulation of BMP7-induced adipogenesis
Title: Fine-tuning BMP7 signalling in adipogenesis by UBE2O/E2-230K-mediated monoubiquitination of SMAD6.

The following UBE2O gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE2O cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17561	NM_022066.4 Latest version!	Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu50576	XM_005257567.1 Latest version!	Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu71127	XM_024450865.1 Latest version!	Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu71128	XM_024450866.1 Latest version!	Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu71129	XM_024450867.1 Latest version!	Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu87027	XM_017024934.1 Latest version!	Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu87028	XM_017024935.1 Latest version!	Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
View Old Accession Versions >>
OHu17561	NM_022066.3	Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBE2O ( NM_022066.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_071349.3 Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), mRNA.

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CloneID	OHu17561
Clone ID Related Accession (Same CDS sequence)	NM_022066.3 , NM_022066.4
Accession Version	NM_022066.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	(E3-independent) E2 ubiquitin-conjugating enzyme
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB051521.1, DA092358.1 and BC025977.1. On Jun 24, 2008 this sequence version replaced NM_022066.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB051521.1, SRR1803617.9431.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGATC CCGCAGCCCC CACGCCCGCA GCTCCCGCTC CAGCCCAGGC CCCGGCTCCA 
GCCCCGGAGG CAGTCCCGGC CCCAGCCGCA GCCCCCGTCC CGGCGCCGGC GCCCGCCTCG 
GACTCGGCCT CCGGGCCGTC CTCGGACTCC GGCCCAGAAG CCGGCTCGCA GCGCCTGCTG 
TTTTCTCACG ACCTGGTGTC GGGCCGTTAC CGTGGCTCCG TGCACTTCGG GCTGGTGCGC 
CTCATCCACG GCGAGGACTC GGACTCGGAG GGCGAGGAGG AGGGCCGCGG GAGCTCGGGG 
TGCTCCGAGG CCGGGGGCGC GGGCCACGAG GAGGGCCGGG CCAGCCCCCT GCGCCGCGGC 
TACGTGCGCG TCCAGTGGTA CCCGGAGGGC GTCAAGCAGC ATGTGAAGGA GACCAAGCTG 
AAACTAGAGG ACCGTTCTGT GGTGCCCCGA GATGTGGTCC GGCACATGCG ATCCACCGAC 
AGTCAGTGTG GCACGGTGAT CGACGTCAAC ATCGACTGTG CCGTCAAGCT CATCGGCACC 
AACTGCATCA TCTATCCCGT CAACAGCAAG GACCTGCAGC ACATCTGGCC CTTCATGTAT 
GGGGACTACA TTGCCTATGA CTGCTGGCTG GGGAAGGTCT ACGACTTGAA GAACCAGATC 
ATCCTGAAGC TATCCAACGG CGCCAGGTGC TCCATGAACA CGGAAGATGG CGCCAAGCTC 
TACGACGTCT GCCCGCACGT CAGCGACTCG GGTCTCTTCT TCGATGATTC CTATGGCTTC 
TACCCAGGCC AGGTGCTCAT TGGCCCTGCC AAGATCTTCT CCAGCGTCCA GTGGCTGTCA 
GGTGTCAAGC CCGTGCTCAG CACCAAGAGC AAGTTCCGAG TGGTGGTGGA AGAGGTGCAG 
GTTGTAGAGT TGAAAGTTAC ATGGATTACC AAGAGTTTCT GTCCAGGGGG CACGGACAGC 
GTCAGCCCCC CACCCTCTGT CATCACCCAG GAAAACCTAG GCAGGGTGAA GCGTCTCGGA 
TGCTTTGACC ATGCTCAGCG GCAGCTTGGG GAGCGCTGTC TGTATGTCTT CCCAGCCAAG 
GTAGAGCCAG CCAAGATTGC CTGGGAATGT CCAGAAAAAA ACTGCGCCCA GGGGGAGGGC 
TCTATGGCCA AGAAGGTGAA GCGCCTGTTG AAGAAGCAGG TTGTGCGGAT CATGTCATGC 
TCCCCAGACA CCCAGTGTTC CCGGGACCAT TCCATGGAAG ACCCAGACAA GAAGGGGGAA 
TCCAAAACCA AGAGCGAAGC GGAGTCTGCC AGCCCTGAGG AGACGCCCGA TGGCTCTGCC 
AGTCCAGTGG AGATGCAGGA CGAGGGTGCA GAGGAGCCCC ACGAGGCAGG AGAGCAGCTG 
CCCCCATTCC TGCTAAAAGA AGGCAGAGAT GACAGGCTGC ACTCGGCAGA GCAGGACGCA 
GATGATGAGG CTGCTGATGA CACGGACGAC ACCAGTTCGG TGACCTCCTC TGCCAGCTCC 
ACCACTTCCT CCCAGAGCGG CAGCGGCACG AGTCGCAAAA AGAGCATCCC CTTGTCCATC 
AAGAACTTAA AGCGCAAACA CAAGAGGAAG AAGAATAAAA TCACTCGAGA CTTCAAGCCA 
GGGGACAGGG TGGCAGTGGA GGTGGTGACC ACGATGACCT CAGCCGACGT GATGTGGCAG 
GATGGCTCCG TGGAATGCAA CATCCGCTCC AACGACCTCT TCCCTGTGCA CCACCTGGAC 
AACAACGAGT TCTGCCCTGG AGACTTCGTG GTAGATAAGC GAGTCCAGAG CTGTCCAGAC 
CCTGCTGTCT ACGGTGTGGT ACAGTCTGGG GACCACATCG GCCGTACCTG CATGGTGAAG 
TGGTTCAAGC TGAGGCCGAG TGGGGACGAC GTGGAGCTGA TTGGAGAAGA GGAAGATGTG 
AGTGTTTACG ACATTGCTGA CCACCCTGAC TTTAGGTTCC GTACAACTGA CATCGTCATC 
CGCATCGGCA ATACTGAGGA TGGGGCTCCT CACAAGGAGG ATGAGCCATC GGTGGGCCAG 
GTGGCCCGTG TGGACGTCAG CAGCAAGGTG GAGGTGGTGT GGGCTGACAA CTCAAAGACC 
ATCATCCTGC CCCAGCACTT GTACAACATA GAGTCTGAGA TTGAGGAGTC AGACTACGAT 
TCGGTAGAAG GCAGCACCAG CGGGGCATCC TCGGATGAAT GGGAAGATGA TAGTGACAGC 
TGGGAGACGG ACAATGGGCT GGTGGAGGAC GAGCACCCCA AGATAGAGGA GCCCCCCATC 
CCACCCCTGG AGCAGCCGGT GGCCCCTGAG GACAAGGGAG TGGTGATCAG TGAAGAGGCA 
GCCACAGCTG CCGTCCAGGG GGCTGTGGCC ATGGCTGCCC CCATGGCCGG GCTGATGGAG 
AAGGCTGGCA AGGACGGGCC ACCCAAGAGC TTCCGGGAGT TGAAAGAGGC CATCAAGATC 
CTGGAGAGCC TCAAGAACAT GACTGTGGAG CAGCTGCTGA CGGGCTCGCC CACCTCTCCG 
ACTGTGGAGC CTGAGAAGCC AACTCGGGAG AAGAAGTTTC TGGATGACAT CAAGAAGCTA 
CAGGAAAACC TCAAGAAGAC CCTGGACAAT GTGGCCATTG TAGAGGAGGA GAAGATGGAA 
GCAGTGCCCG ACGTAGAGCG CAAGGAGGAC AAGCCCGAGG GGCAGTCACC TGTGAAGGCT 
GAGTGGCCCA GCGAAACCCC GGTGCTGTGC CAGCAGTGTG GCGGCAAGCC TGGCGTCACC 
TTCACCAGCG CCAAGGGCGA GGTCTTCTCC GTACTGGAGT TTGCACCCTC AAATCATTCT 
TTTAAGAAAA TTGAGTTCCA GCCTCCAGAA GCCAAGAAGT TCTTCAGCAC AGTGCGGAAG 
GAGATGGCGC TGCTGGCTAC CTCACTGCCT GAGGGCATCA TGGTCAAGAC TTTTGAAGAT 
AGAATGGACC TCTTCTCAGC TCTCATCAAG GGCCCCACTC GAACCCCCTA CGAGGATGGC 
CTCTACTTGT TTGACATCCA GCTCCCCAAC ATCTACCCAG CCGTGCCCCC CCACTTCTGC 
TACCTCTCCC AATGCAGTGG CCGCCTGAAC CCCAACCTGT ATGACAATGG GAAGGTGTGT 
GTCAGCCTCC TGGGCACCTG GATTGGAAAG GGGACAGAGA GGTGGACAAG CAAGTCCAGC 
CTTCTCCAGG TGCTCATCTC CATCCAAGGT CTGATCCTGG TAAATGAACC ATACTACAAC 
GAAGCCGGCT TCGACAGTGA CCGAGGCCTG CAGGAAGGCT ATGAAAACAG TCGCTGTTAC 
AATGAGATGG CGCTGATCCG CGTGGTGCAG TCCATGACCC AGCTGGTGCG GCGGCCCCCC 
GAGGTCTTTG AGCAGGAGAT CAGGCAACAC TTTAGCACTG GTGGCTGGCG GCTGGTGAAC 
CGTATCGAGT CCTGGCTGGA AACCCATGCC CTGCTGGAGA AGGCCCAGGC ACTGCCCAAC 
GGGGTGCCCA AGGCCAGCAG CTCGCCAGAG CCCCCAGCTG TAGCCGAGCT GTCAGACTCC 
GGCCAACAAG AACCTGAGGA TGGAGGGCCA GCCCCAGGAG AGGCCTCCCA GGGCTCAGAC 
TCAGAGGGCG GTGCCCAGGG CCTGGCCTCA GCTAGCAGGG ACCACACAGA CCAGACTTCG 
GAGACCGCAC CAGACGCATC GGTGCCACCC AGTGTGAAAC CAAAGAAGCG GAGAAAGAGC 
TACCGGAGCT TCTTACCTGA GAAGAGTGGC TACCCTGACA TCGGCTTCCC CCTCTTCCCA 
CTTTCCAAGG GTTTCATCAA GAGCATCCGG GGTGTCCTGA CGCAGTTCCG GGCTGCCCTG 
CTAGAGGCAG GCATGCCGGA GTGCACAGAG GACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071349.3
CDS	66..3944
Translation	MADPAAPTPAAPAPAQAPAPAPEAVPAPAAAPVPAPAPASDSASGPSSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSDSEGEEEGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPAKIAWECPEKNCAQGEGSMAKKVKRLLKKQVVRIMSCSPDTQCSRDHSMEDPDKKGESKTKSEAESASPEETPDGSASPVEMQDEGAEEPHEAGEQLPPFLLKEGRDDRLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGTSRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHIGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAPHKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIEEPPIPPLEQPVAPEDKGVVISEEAATAAVQGAVAMAAPMAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIVEEEKMEAVPDVERKEDKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPEGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWLETHALLEKAQALPNGVPKASSSPEPPAVAELSDSGQQEPEDGGPAPGEASQGSDSEGGAQGLASASRDHTDQTSETAPDASVPPSVKPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALLEAGMPECTEDK

Target ORF information:

RefSeq Version	NM_022066.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022066.3

ORF Insert Sequence:

ATGGCGGATC CCGCAGCCCC CACGCCCGCA GCTCCCGCTC CAGCCCAGGC CCCGGCTCCA 
GCCCCGGAGG CAGTCCCGGC CCCAGCCGCA GCCCCCGTCC CGGCGCCGGC GCCCGCCTCG 
GACTCGGCCT CCGGGCCGTC CTCGGACTCC GGCCCAGAAG CCGGCTCGCA GCGCCTGCTG 
TTTTCTCACG ACCTGGTGTC GGGCCGTTAC CGTGGCTCCG TGCACTTCGG GCTGGTGCGC 
CTCATCCACG GCGAGGACTC GGACTCGGAG GGCGAGGAGG AGGGCCGCGG GAGCTCGGGG 
TGCTCCGAGG CCGGGGGCGC GGGCCACGAG GAGGGCCGGG CCAGCCCCCT GCGCCGCGGC 
TACGTGCGCG TCCAGTGGTA CCCGGAGGGC GTCAAGCAGC ATGTGAAGGA GACCAAGCTG 
AAACTAGAGG ACCGTTCTGT GGTGCCCCGA GATGTGGTCC GGCACATGCG ATCCACCGAC 
AGTCAGTGTG GCACGGTGAT CGACGTCAAC ATCGACTGTG CCGTCAAGCT CATCGGCACC 
AACTGCATCA TCTATCCCGT CAACAGCAAG GACCTGCAGC ACATCTGGCC CTTCATGTAT 
GGGGACTACA TTGCCTATGA CTGCTGGCTG GGGAAGGTCT ACGACTTGAA GAACCAGATC 
ATCCTGAAGC TATCCAACGG CGCCAGGTGC TCCATGAACA CGGAAGATGG CGCCAAGCTC 
TACGACGTCT GCCCGCACGT CAGCGACTCG GGTCTCTTCT TCGATGATTC CTATGGCTTC 
TACCCAGGCC AGGTGCTCAT TGGCCCTGCC AAGATCTTCT CCAGCGTCCA GTGGCTGTCA 
GGTGTCAAGC CCGTGCTCAG CACCAAGAGC AAGTTCCGAG TGGTGGTGGA AGAGGTGCAG 
GTTGTAGAGT TGAAAGTTAC ATGGATTACC AAGAGTTTCT GTCCAGGGGG CACGGACAGC 
GTCAGCCCCC CACCCTCTGT CATCACCCAG GAAAACCTAG GCAGGGTGAA GCGTCTCGGA 
TGCTTTGACC ATGCTCAGCG GCAGCTTGGG GAGCGCTGTC TGTATGTCTT CCCAGCCAAG 
GTAGAGCCAG CCAAGATTGC CTGGGAATGT CCAGAAAAAA ACTGCGCCCA GGGGGAGGGC 
TCTATGGCCA AGAAGGTGAA GCGCCTGTTG AAGAAGCAGG TTGTGCGGAT CATGTCATGC 
TCCCCAGACA CCCAGTGTTC CCGGGACCAT TCCATGGAAG ACCCAGACAA GAAGGGGGAA 
TCCAAAACCA AGAGCGAAGC GGAGTCTGCC AGCCCTGAGG AGACGCCCGA TGGCTCTGCC 
AGTCCAGTGG AGATGCAGGA CGAGGGTGCA GAGGAGCCCC ACGAGGCAGG AGAGCAGCTG 
CCCCCATTCC TGCTAAAAGA AGGCAGAGAT GACAGGCTGC ACTCGGCAGA GCAGGACGCA 
GATGATGAGG CTGCTGATGA CACGGACGAC ACCAGTTCGG TGACCTCCTC TGCCAGCTCC 
ACCACTTCCT CCCAGAGCGG CAGCGGCACG AGTCGCAAAA AGAGCATCCC CTTGTCCATC 
AAGAACTTAA AGCGCAAACA CAAGAGGAAG AAGAATAAAA TCACTCGAGA CTTCAAGCCA 
GGGGACAGGG TGGCAGTGGA GGTGGTGACC ACGATGACCT CAGCCGACGT GATGTGGCAG 
GATGGCTCCG TGGAATGCAA CATCCGCTCC AACGACCTCT TCCCTGTGCA CCACCTGGAC 
AACAACGAGT TCTGCCCTGG AGACTTCGTG GTAGATAAGC GAGTCCAGAG CTGTCCAGAC 
CCTGCTGTCT ACGGTGTGGT ACAGTCTGGG GACCACATCG GCCGTACCTG CATGGTGAAG 
TGGTTCAAGC TGAGGCCGAG TGGGGACGAC GTGGAGCTGA TTGGAGAAGA GGAAGATGTG 
AGTGTTTACG ACATTGCTGA CCACCCTGAC TTTAGGTTCC GTACAACTGA CATCGTCATC 
CGCATCGGCA ATACTGAGGA TGGGGCTCCT CACAAGGAGG ATGAGCCATC GGTGGGCCAG 
GTGGCCCGTG TGGACGTCAG CAGCAAGGTG GAGGTGGTGT GGGCTGACAA CTCAAAGACC 
ATCATCCTGC CCCAGCACTT GTACAACATA GAGTCTGAGA TTGAGGAGTC AGACTACGAT 
TCGGTAGAAG GCAGCACCAG CGGGGCATCC TCGGATGAAT GGGAAGATGA TAGTGACAGC 
TGGGAGACGG ACAATGGGCT GGTGGAGGAC GAGCACCCCA AGATAGAGGA GCCCCCCATC 
CCACCCCTGG AGCAGCCGGT GGCCCCTGAG GACAAGGGAG TGGTGATCAG TGAAGAGGCA 
GCCACAGCTG CCGTCCAGGG GGCTGTGGCC ATGGCTGCCC CCATGGCCGG GCTGATGGAG 
AAGGCTGGCA AGGACGGGCC ACCCAAGAGC TTCCGGGAGT TGAAAGAGGC CATCAAGATC 
CTGGAGAGCC TCAAGAACAT GACTGTGGAG CAGCTGCTGA CGGGCTCGCC CACCTCTCCG 
ACTGTGGAGC CTGAGAAGCC AACTCGGGAG AAGAAGTTTC TGGATGACAT CAAGAAGCTA 
CAGGAAAACC TCAAGAAGAC CCTGGACAAT GTGGCCATTG TAGAGGAGGA GAAGATGGAA 
GCAGTGCCCG ACGTAGAGCG CAAGGAGGAC AAGCCCGAGG GGCAGTCACC TGTGAAGGCT 
GAGTGGCCCA GCGAAACCCC GGTGCTGTGC CAGCAGTGTG GCGGCAAGCC TGGCGTCACC 
TTCACCAGCG CCAAGGGCGA GGTCTTCTCC GTACTGGAGT TTGCACCCTC AAATCATTCT 
TTTAAGAAAA TTGAGTTCCA GCCTCCAGAA GCCAAGAAGT TCTTCAGCAC AGTGCGGAAG 
GAGATGGCGC TGCTGGCTAC CTCACTGCCT GAGGGCATCA TGGTCAAGAC TTTTGAAGAT 
AGAATGGACC TCTTCTCAGC TCTCATCAAG GGCCCCACTC GAACCCCCTA CGAGGATGGC 
CTCTACTTGT TTGACATCCA GCTCCCCAAC ATCTACCCAG CCGTGCCCCC CCACTTCTGC 
TACCTCTCCC AATGCAGTGG CCGCCTGAAC CCCAACCTGT ATGACAATGG GAAGGTGTGT 
GTCAGCCTCC TGGGCACCTG GATTGGAAAG GGGACAGAGA GGTGGACAAG CAAGTCCAGC 
CTTCTCCAGG TGCTCATCTC CATCCAAGGT CTGATCCTGG TAAATGAACC ATACTACAAC 
GAAGCCGGCT TCGACAGTGA CCGAGGCCTG CAGGAAGGCT ATGAAAACAG TCGCTGTTAC 
AATGAGATGG CGCTGATCCG CGTGGTGCAG TCCATGACCC AGCTGGTGCG GCGGCCCCCC 
GAGGTCTTTG AGCAGGAGAT CAGGCAACAC TTTAGCACTG GTGGCTGGCG GCTGGTGAAC 
CGTATCGAGT CCTGGCTGGA AACCCATGCC CTGCTGGAGA AGGCCCAGGC ACTGCCCAAC 
GGGGTGCCCA AGGCCAGCAG CTCGCCAGAG CCCCCAGCTG TAGCCGAGCT GTCAGACTCC 
GGCCAACAAG AACCTGAGGA TGGAGGGCCA GCCCCAGGAG AGGCCTCCCA GGGCTCAGAC 
TCAGAGGGCG GTGCCCAGGG CCTGGCCTCA GCTAGCAGGG ACCACACAGA CCAGACTTCG 
GAGACCGCAC CAGACGCATC GGTGCCACCC AGTGTGAAAC CAAAGAAGCG GAGAAAGAGC 
TACCGGAGCT TCTTACCTGA GAAGAGTGGC TACCCTGACA TCGGCTTCCC CCTCTTCCCA 
CTTTCCAAGG GTTTCATCAA GAGCATCCGG GGTGTCCTGA CGCAGTTCCG GGCTGCCCTG 
CTAGAGGCAG GCATGCCGGA GTGCACAGAG GACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2O ( NM_022066.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_071349.3 Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), mRNA.

Price & Availability↑

CloneID	OHu17561
Clone ID Related Accession (Same CDS sequence)	NM_022066.3 , NM_022066.4
Accession Version	NM_022066.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	(E3-independent) E2 ubiquitin-conjugating enzyme
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB051521.1, DA092358.1, BC025977.1 and AC090699.10. On Nov 23, 2018 this sequence version replaced NM_022066.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.9431.1, SRR1660809.204905.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000319380.12/ ENSP00000323687.6 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGATC CCGCAGCCCC CACGCCCGCA GCTCCCGCTC CAGCCCAGGC CCCGGCTCCA 
GCCCCGGAGG CAGTCCCGGC CCCAGCCGCA GCCCCCGTCC CGGCGCCGGC GCCCGCCTCG 
GACTCGGCCT CCGGGCCGTC CTCGGACTCC GGCCCAGAAG CCGGCTCGCA GCGCCTGCTG 
TTTTCTCACG ACCTGGTGTC GGGCCGTTAC CGTGGCTCCG TGCACTTCGG GCTGGTGCGC 
CTCATCCACG GCGAGGACTC GGACTCGGAG GGCGAGGAGG AGGGCCGCGG GAGCTCGGGG 
TGCTCCGAGG CCGGGGGCGC GGGCCACGAG GAGGGCCGGG CCAGCCCCCT GCGCCGCGGC 
TACGTGCGCG TCCAGTGGTA CCCGGAGGGC GTCAAGCAGC ATGTGAAGGA GACCAAGCTG 
AAACTAGAGG ACCGTTCTGT GGTGCCCCGA GATGTGGTCC GGCACATGCG ATCCACCGAC 
AGTCAGTGTG GCACGGTGAT CGACGTCAAC ATCGACTGTG CCGTCAAGCT CATCGGCACC 
AACTGCATCA TCTATCCCGT CAACAGCAAG GACCTGCAGC ACATCTGGCC CTTCATGTAT 
GGGGACTACA TTGCCTATGA CTGCTGGCTG GGGAAGGTCT ACGACTTGAA GAACCAGATC 
ATCCTGAAGC TATCCAACGG CGCCAGGTGC TCCATGAACA CGGAAGATGG CGCCAAGCTC 
TACGACGTCT GCCCGCACGT CAGCGACTCG GGTCTCTTCT TCGATGATTC CTATGGCTTC 
TACCCAGGCC AGGTGCTCAT TGGCCCTGCC AAGATCTTCT CCAGCGTCCA GTGGCTGTCA 
GGTGTCAAGC CCGTGCTCAG CACCAAGAGC AAGTTCCGAG TGGTGGTGGA AGAGGTGCAG 
GTTGTAGAGT TGAAAGTTAC ATGGATTACC AAGAGTTTCT GTCCAGGGGG CACGGACAGC 
GTCAGCCCCC CACCCTCTGT CATCACCCAG GAAAACCTAG GCAGGGTGAA GCGTCTCGGA 
TGCTTTGACC ATGCTCAGCG GCAGCTTGGG GAGCGCTGTC TGTATGTCTT CCCAGCCAAG 
GTAGAGCCAG CCAAGATTGC CTGGGAATGT CCAGAAAAAA ACTGCGCCCA GGGGGAGGGC 
TCTATGGCCA AGAAGGTGAA GCGCCTGTTG AAGAAGCAGG TTGTGCGGAT CATGTCATGC 
TCCCCAGACA CCCAGTGTTC CCGGGACCAT TCCATGGAAG ACCCAGACAA GAAGGGGGAA 
TCCAAAACCA AGAGCGAAGC GGAGTCTGCC AGCCCTGAGG AGACGCCCGA TGGCTCTGCC 
AGTCCAGTGG AGATGCAGGA CGAGGGTGCA GAGGAGCCCC ACGAGGCAGG AGAGCAGCTG 
CCCCCATTCC TGCTAAAAGA AGGCAGAGAT GACAGGCTGC ACTCGGCAGA GCAGGACGCA 
GATGATGAGG CTGCTGATGA CACGGACGAC ACCAGTTCGG TGACCTCCTC TGCCAGCTCC 
ACCACTTCCT CCCAGAGCGG CAGCGGCACG AGTCGCAAAA AGAGCATCCC CTTGTCCATC 
AAGAACTTAA AGCGCAAACA CAAGAGGAAG AAGAATAAAA TCACTCGAGA CTTCAAGCCA 
GGGGACAGGG TGGCAGTGGA GGTGGTGACC ACGATGACCT CAGCCGACGT GATGTGGCAG 
GATGGCTCCG TGGAATGCAA CATCCGCTCC AACGACCTCT TCCCTGTGCA CCACCTGGAC 
AACAACGAGT TCTGCCCTGG AGACTTCGTG GTAGATAAGC GAGTCCAGAG CTGTCCAGAC 
CCTGCTGTCT ACGGTGTGGT ACAGTCTGGG GACCACATCG GCCGTACCTG CATGGTGAAG 
TGGTTCAAGC TGAGGCCGAG TGGGGACGAC GTGGAGCTGA TTGGAGAAGA GGAAGATGTG 
AGTGTTTACG ACATTGCTGA CCACCCTGAC TTTAGGTTCC GTACAACTGA CATCGTCATC 
CGCATCGGCA ATACTGAGGA TGGGGCTCCT CACAAGGAGG ATGAGCCATC GGTGGGCCAG 
GTGGCCCGTG TGGACGTCAG CAGCAAGGTG GAGGTGGTGT GGGCTGACAA CTCAAAGACC 
ATCATCCTGC CCCAGCACTT GTACAACATA GAGTCTGAGA TTGAGGAGTC AGACTACGAT 
TCGGTAGAAG GCAGCACCAG CGGGGCATCC TCGGATGAAT GGGAAGATGA TAGTGACAGC 
TGGGAGACGG ACAATGGGCT GGTGGAGGAC GAGCACCCCA AGATAGAGGA GCCCCCCATC 
CCACCCCTGG AGCAGCCGGT GGCCCCTGAG GACAAGGGAG TGGTGATCAG TGAAGAGGCA 
GCCACAGCTG CCGTCCAGGG GGCTGTGGCC ATGGCTGCCC CCATGGCCGG GCTGATGGAG 
AAGGCTGGCA AGGACGGGCC ACCCAAGAGC TTCCGGGAGT TGAAAGAGGC CATCAAGATC 
CTGGAGAGCC TCAAGAACAT GACTGTGGAG CAGCTGCTGA CGGGCTCGCC CACCTCTCCG 
ACTGTGGAGC CTGAGAAGCC AACTCGGGAG AAGAAGTTTC TGGATGACAT CAAGAAGCTA 
CAGGAAAACC TCAAGAAGAC CCTGGACAAT GTGGCCATTG TAGAGGAGGA GAAGATGGAA 
GCAGTGCCCG ACGTAGAGCG CAAGGAGGAC AAGCCCGAGG GGCAGTCACC TGTGAAGGCT 
GAGTGGCCCA GCGAAACCCC GGTGCTGTGC CAGCAGTGTG GCGGCAAGCC TGGCGTCACC 
TTCACCAGCG CCAAGGGCGA GGTCTTCTCC GTACTGGAGT TTGCACCCTC AAATCATTCT 
TTTAAGAAAA TTGAGTTCCA GCCTCCAGAA GCCAAGAAGT TCTTCAGCAC AGTGCGGAAG 
GAGATGGCGC TGCTGGCTAC CTCACTGCCT GAGGGCATCA TGGTCAAGAC TTTTGAAGAT 
AGAATGGACC TCTTCTCAGC TCTCATCAAG GGCCCCACTC GAACCCCCTA CGAGGATGGC 
CTCTACTTGT TTGACATCCA GCTCCCCAAC ATCTACCCAG CCGTGCCCCC CCACTTCTGC 
TACCTCTCCC AATGCAGTGG CCGCCTGAAC CCCAACCTGT ATGACAATGG GAAGGTGTGT 
GTCAGCCTCC TGGGCACCTG GATTGGAAAG GGGACAGAGA GGTGGACAAG CAAGTCCAGC 
CTTCTCCAGG TGCTCATCTC CATCCAAGGT CTGATCCTGG TAAATGAACC ATACTACAAC 
GAAGCCGGCT TCGACAGTGA CCGAGGCCTG CAGGAAGGCT ATGAAAACAG TCGCTGTTAC 
AATGAGATGG CGCTGATCCG CGTGGTGCAG TCCATGACCC AGCTGGTGCG GCGGCCCCCC 
GAGGTCTTTG AGCAGGAGAT CAGGCAACAC TTTAGCACTG GTGGCTGGCG GCTGGTGAAC 
CGTATCGAGT CCTGGCTGGA AACCCATGCC CTGCTGGAGA AGGCCCAGGC ACTGCCCAAC 
GGGGTGCCCA AGGCCAGCAG CTCGCCAGAG CCCCCAGCTG TAGCCGAGCT GTCAGACTCC 
GGCCAACAAG AACCTGAGGA TGGAGGGCCA GCCCCAGGAG AGGCCTCCCA GGGCTCAGAC 
TCAGAGGGCG GTGCCCAGGG CCTGGCCTCA GCTAGCAGGG ACCACACAGA CCAGACTTCG 
GAGACCGCAC CAGACGCATC GGTGCCACCC AGTGTGAAAC CAAAGAAGCG GAGAAAGAGC 
TACCGGAGCT TCTTACCTGA GAAGAGTGGC TACCCTGACA TCGGCTTCCC CCTCTTCCCA 
CTTTCCAAGG GTTTCATCAA GAGCATCCGG GGTGTCCTGA CGCAGTTCCG GGCTGCCCTG 
CTAGAGGCAG GCATGCCGGA GTGCACAGAG GACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071349.3
CDS	12..3890
Translation	MADPAAPTPAAPAPAQAPAPAPEAVPAPAAAPVPAPAPASDSASGPSSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSDSEGEEEGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPAKIAWECPEKNCAQGEGSMAKKVKRLLKKQVVRIMSCSPDTQCSRDHSMEDPDKKGESKTKSEAESASPEETPDGSASPVEMQDEGAEEPHEAGEQLPPFLLKEGRDDRLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGTSRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHIGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAPHKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIEEPPIPPLEQPVAPEDKGVVISEEAATAAVQGAVAMAAPMAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIVEEEKMEAVPDVERKEDKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPEGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWLETHALLEKAQALPNGVPKASSSPEPPAVAELSDSGQQEPEDGGPAPGEASQGSDSEGGAQGLASASRDHTDQTSETAPDASVPPSVKPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALLEAGMPECTEDK

Target ORF information:

RefSeq Version	NM_022066.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022066.4

ORF Insert Sequence:

ATGGCGGATC CCGCAGCCCC CACGCCCGCA GCTCCCGCTC CAGCCCAGGC CCCGGCTCCA 
GCCCCGGAGG CAGTCCCGGC CCCAGCCGCA GCCCCCGTCC CGGCGCCGGC GCCCGCCTCG 
GACTCGGCCT CCGGGCCGTC CTCGGACTCC GGCCCAGAAG CCGGCTCGCA GCGCCTGCTG 
TTTTCTCACG ACCTGGTGTC GGGCCGTTAC CGTGGCTCCG TGCACTTCGG GCTGGTGCGC 
CTCATCCACG GCGAGGACTC GGACTCGGAG GGCGAGGAGG AGGGCCGCGG GAGCTCGGGG 
TGCTCCGAGG CCGGGGGCGC GGGCCACGAG GAGGGCCGGG CCAGCCCCCT GCGCCGCGGC 
TACGTGCGCG TCCAGTGGTA CCCGGAGGGC GTCAAGCAGC ATGTGAAGGA GACCAAGCTG 
AAACTAGAGG ACCGTTCTGT GGTGCCCCGA GATGTGGTCC GGCACATGCG ATCCACCGAC 
AGTCAGTGTG GCACGGTGAT CGACGTCAAC ATCGACTGTG CCGTCAAGCT CATCGGCACC 
AACTGCATCA TCTATCCCGT CAACAGCAAG GACCTGCAGC ACATCTGGCC CTTCATGTAT 
GGGGACTACA TTGCCTATGA CTGCTGGCTG GGGAAGGTCT ACGACTTGAA GAACCAGATC 
ATCCTGAAGC TATCCAACGG CGCCAGGTGC TCCATGAACA CGGAAGATGG CGCCAAGCTC 
TACGACGTCT GCCCGCACGT CAGCGACTCG GGTCTCTTCT TCGATGATTC CTATGGCTTC 
TACCCAGGCC AGGTGCTCAT TGGCCCTGCC AAGATCTTCT CCAGCGTCCA GTGGCTGTCA 
GGTGTCAAGC CCGTGCTCAG CACCAAGAGC AAGTTCCGAG TGGTGGTGGA AGAGGTGCAG 
GTTGTAGAGT TGAAAGTTAC ATGGATTACC AAGAGTTTCT GTCCAGGGGG CACGGACAGC 
GTCAGCCCCC CACCCTCTGT CATCACCCAG GAAAACCTAG GCAGGGTGAA GCGTCTCGGA 
TGCTTTGACC ATGCTCAGCG GCAGCTTGGG GAGCGCTGTC TGTATGTCTT CCCAGCCAAG 
GTAGAGCCAG CCAAGATTGC CTGGGAATGT CCAGAAAAAA ACTGCGCCCA GGGGGAGGGC 
TCTATGGCCA AGAAGGTGAA GCGCCTGTTG AAGAAGCAGG TTGTGCGGAT CATGTCATGC 
TCCCCAGACA CCCAGTGTTC CCGGGACCAT TCCATGGAAG ACCCAGACAA GAAGGGGGAA 
TCCAAAACCA AGAGCGAAGC GGAGTCTGCC AGCCCTGAGG AGACGCCCGA TGGCTCTGCC 
AGTCCAGTGG AGATGCAGGA CGAGGGTGCA GAGGAGCCCC ACGAGGCAGG AGAGCAGCTG 
CCCCCATTCC TGCTAAAAGA AGGCAGAGAT GACAGGCTGC ACTCGGCAGA GCAGGACGCA 
GATGATGAGG CTGCTGATGA CACGGACGAC ACCAGTTCGG TGACCTCCTC TGCCAGCTCC 
ACCACTTCCT CCCAGAGCGG CAGCGGCACG AGTCGCAAAA AGAGCATCCC CTTGTCCATC 
AAGAACTTAA AGCGCAAACA CAAGAGGAAG AAGAATAAAA TCACTCGAGA CTTCAAGCCA 
GGGGACAGGG TGGCAGTGGA GGTGGTGACC ACGATGACCT CAGCCGACGT GATGTGGCAG 
GATGGCTCCG TGGAATGCAA CATCCGCTCC AACGACCTCT TCCCTGTGCA CCACCTGGAC 
AACAACGAGT TCTGCCCTGG AGACTTCGTG GTAGATAAGC GAGTCCAGAG CTGTCCAGAC 
CCTGCTGTCT ACGGTGTGGT ACAGTCTGGG GACCACATCG GCCGTACCTG CATGGTGAAG 
TGGTTCAAGC TGAGGCCGAG TGGGGACGAC GTGGAGCTGA TTGGAGAAGA GGAAGATGTG 
AGTGTTTACG ACATTGCTGA CCACCCTGAC TTTAGGTTCC GTACAACTGA CATCGTCATC 
CGCATCGGCA ATACTGAGGA TGGGGCTCCT CACAAGGAGG ATGAGCCATC GGTGGGCCAG 
GTGGCCCGTG TGGACGTCAG CAGCAAGGTG GAGGTGGTGT GGGCTGACAA CTCAAAGACC 
ATCATCCTGC CCCAGCACTT GTACAACATA GAGTCTGAGA TTGAGGAGTC AGACTACGAT 
TCGGTAGAAG GCAGCACCAG CGGGGCATCC TCGGATGAAT GGGAAGATGA TAGTGACAGC 
TGGGAGACGG ACAATGGGCT GGTGGAGGAC GAGCACCCCA AGATAGAGGA GCCCCCCATC 
CCACCCCTGG AGCAGCCGGT GGCCCCTGAG GACAAGGGAG TGGTGATCAG TGAAGAGGCA 
GCCACAGCTG CCGTCCAGGG GGCTGTGGCC ATGGCTGCCC CCATGGCCGG GCTGATGGAG 
AAGGCTGGCA AGGACGGGCC ACCCAAGAGC TTCCGGGAGT TGAAAGAGGC CATCAAGATC 
CTGGAGAGCC TCAAGAACAT GACTGTGGAG CAGCTGCTGA CGGGCTCGCC CACCTCTCCG 
ACTGTGGAGC CTGAGAAGCC AACTCGGGAG AAGAAGTTTC TGGATGACAT CAAGAAGCTA 
CAGGAAAACC TCAAGAAGAC CCTGGACAAT GTGGCCATTG TAGAGGAGGA GAAGATGGAA 
GCAGTGCCCG ACGTAGAGCG CAAGGAGGAC AAGCCCGAGG GGCAGTCACC TGTGAAGGCT 
GAGTGGCCCA GCGAAACCCC GGTGCTGTGC CAGCAGTGTG GCGGCAAGCC TGGCGTCACC 
TTCACCAGCG CCAAGGGCGA GGTCTTCTCC GTACTGGAGT TTGCACCCTC AAATCATTCT 
TTTAAGAAAA TTGAGTTCCA GCCTCCAGAA GCCAAGAAGT TCTTCAGCAC AGTGCGGAAG 
GAGATGGCGC TGCTGGCTAC CTCACTGCCT GAGGGCATCA TGGTCAAGAC TTTTGAAGAT 
AGAATGGACC TCTTCTCAGC TCTCATCAAG GGCCCCACTC GAACCCCCTA CGAGGATGGC 
CTCTACTTGT TTGACATCCA GCTCCCCAAC ATCTACCCAG CCGTGCCCCC CCACTTCTGC 
TACCTCTCCC AATGCAGTGG CCGCCTGAAC CCCAACCTGT ATGACAATGG GAAGGTGTGT 
GTCAGCCTCC TGGGCACCTG GATTGGAAAG GGGACAGAGA GGTGGACAAG CAAGTCCAGC 
CTTCTCCAGG TGCTCATCTC CATCCAAGGT CTGATCCTGG TAAATGAACC ATACTACAAC 
GAAGCCGGCT TCGACAGTGA CCGAGGCCTG CAGGAAGGCT ATGAAAACAG TCGCTGTTAC 
AATGAGATGG CGCTGATCCG CGTGGTGCAG TCCATGACCC AGCTGGTGCG GCGGCCCCCC 
GAGGTCTTTG AGCAGGAGAT CAGGCAACAC TTTAGCACTG GTGGCTGGCG GCTGGTGAAC 
CGTATCGAGT CCTGGCTGGA AACCCATGCC CTGCTGGAGA AGGCCCAGGC ACTGCCCAAC 
GGGGTGCCCA AGGCCAGCAG CTCGCCAGAG CCCCCAGCTG TAGCCGAGCT GTCAGACTCC 
GGCCAACAAG AACCTGAGGA TGGAGGGCCA GCCCCAGGAG AGGCCTCCCA GGGCTCAGAC 
TCAGAGGGCG GTGCCCAGGG CCTGGCCTCA GCTAGCAGGG ACCACACAGA CCAGACTTCG 
GAGACCGCAC CAGACGCATC GGTGCCACCC AGTGTGAAAC CAAAGAAGCG GAGAAAGAGC 
TACCGGAGCT TCTTACCTGA GAAGAGTGGC TACCCTGACA TCGGCTTCCC CCTCTTCCCA 
CTTTCCAAGG GTTTCATCAA GAGCATCCGG GGTGTCCTGA CGCAGTTCCG GGCTGCCCTG 
CTAGAGGCAG GCATGCCGGA GTGCACAGAG GACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2O ( XM_005257567.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005257624.1 Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X4, mRNA.

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CloneID	OHu50576
Clone ID Related Accession (Same CDS sequence)	XM_005257567.1
Accession Version	XM_005257567.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATC CCGCAGCCCC CACGCCCGCA GCTCCCGCTC CAGCCCAGGC CCCGGCTCCA 
GCCCCGGAGG CAGTCCCGGC CCCAGCCGCA GCCCCCGTCC CGGCGCCGGC GCCCGCCTCG 
GACTCGGCCT CCGGGCCGTC CTCGGACTCC GGCCCAGAAG CCGGCTCGCA GCGCCTGCTG 
TTTTCTCACG ACCTGGTGTC GGGCCGTTAC CGTGGCTCCG TGCACTTCGG GCTGGTGCGC 
CTCATCCACG GCGAGGACTC GGACTCGGAG GGCGAGGAGG AGGGCCGCGG GAGCTCGGGG 
TGCTCCGAGG CCGGGGGCGC GGGCCACGAG GAGGGCCGGG CCAGCCCCCT GCGCCGCGGC 
TACGTGCGCG TCCAGTGGTA CCCGGAGGGC GTCAAGCAGC ATGTGAAGGA GACCAAGCTG 
AAACTAGAGG ACCGTTCTGT GGTGCCCCGA GATGTGGTCC GGCACATGCG ATCCACCGAC 
AGTCAGTGTG GCACGGTGAT CGACGTCAAC ATCGACTGTG CCGTCAAGCT CATCGGCACC 
AACTGCATCA TCTATCCCGT CAACAGCAAG GACCTGCAGC ACATCTGGCC CTTCATGTAT 
GGGGACTACA TTGCCTATGA CTGCTGGCTG GGGAAGGTCT ACGACTTGAA GAACCAGATC 
ATCCTGAAGC TATCCAACGG CGCCAGGTGC TCCATGAACA CGGAAGATGG CGCCAAGCTC 
TACGACGTCT GCCCGCACGT CAGCGACTCG GGTCTCTTCT TCGATGATTC CTATGGCTTC 
TACCCAGGCC AGGTGCTCAT TGGCCCTGCC AAGATCTTCT CCAGCGTCCA GTGGCTGTCA 
GGTGTCAAGC CCGTGCTCAG CACCAAGAGC AAGTTCCGAG TGGTGGTGGA AGAGGTGCAG 
GTTGTAGAGT TGAAAGTTAC ATGGATTACC AAGAGTTTCT GTCCAGGGGG CACGGACAGC 
GTCAGCCCCC CACCCTCTGT CATCACCCAG GAAAACCTAG GCAGGGTGAA GCGTCTCGGA 
TGCTTTGACC ATGCTCAGCG GCAGCTTGGG GAGCGCTGTC TGTATGTCTT CCCAGCCAAG 
GTAGAGCCAG CCAAGATTGC CTGGGAATGT CCAGAAAAAA ACTGCGCCCA GGGGGAGGGC 
TCTATGGCCA AGAAGGTGAA GCGCCTGTTG AAGAAGCAGG TTGTGCGGAT CATGTCATGC 
TCCCCAGACA CCCAGTGTTC CCGGGACCAT TCCATGGAAG ACCCAGACAA GAAGGGGGAA 
TCCAAAACCA AGAGCGAAGC GGAGTCTGCC AGCCCTGAGG AGACGCCCGA TGGCTCTGCC 
AGTCCAGTGG AGATGCAGGA CGAGGGTGCA GAGGAGCCCC ACGAGGCAGG AGAGCAGCTG 
CCCCCATTCC TGCTAAAAGA AGGCAGAGAT GACAGGCTGC ACTCGGCAGA GCAGGACGCA 
GATGATGAGG CTGCTGATGA CACGGACGAC ACCAGTTCGG TGACCTCCTC TGCCAGCTCC 
ACCACTTCCT CCCAGAGCGG CAGCGGCACG AGTCGCAAAA AGAGCATCCC CTTGTCCATC 
AAGAACTTAA AGCGCAAACA CAAGAGGAAG AAGAATAAAA TCACTCGAGA CTTCAAGCCA 
GGGGACAGGG TGGCAGTGGA GGTGGTGACC ACGATGACCT CAGCCGACGT GATGTGGCAG 
GATGGCTCCG TGGAATGCAA CATCCGCTCC AACGACCTCT TCCCTGTGCA CCACCTGGAC 
AACAACGAGT TCTGCCCTGG AGACTTCGTG GTAGATAAGC GAGTCCAGAG CTGTCCAGAC 
CCTGCTGTCT ACGGTGTGGT ACAGTCTGGG GACCACATCG GCCGTACCTG CATGGTGAAG 
TGGTTCAAGC TGAGGCCGAG TGGGGACGAC GTGGAGCTGA TTGGAGAAGA GGAAGATGTG 
AGTGTTTACG ACATTGCTGA CCACCCTGAC TTTAGGTTCC GTACAACTGA CATCGTCATC 
CGCATCGGCA ATACTGAGGA TGGGGCTCCT CACAAGGAGG ATGAGCAGCC ATCGGTGGGC 
CAGGTGGCCC GTGTGGACGT CAGCAGCAAG GTGGAGGTGG TGTGGGCTGA CAACTCAAAG 
ACCATCATCC TGCCCCAGCA CTTGTACAAC ATAGAGTCTG AGATTGAGGA GTCAGACTAC 
GATTCGGTAG AAGGCAGCAC CAGCGGGGCA TCCTCGGATG AATGGGAAGA TGATAGTGAC 
AGCTGGGAGA CGGACAATGG GCTGGTGGAG GACGAGCACC CCAAGATAGA GGAGCCCCCC 
ATCCCACCCC TGGAGCAGCC GGTGGCCCCT GAGGACAAGG GAGTGGTGAT CAGTGAAGAG 
GCAGCCACAG CTGCCGTCCA GGGGGCTGTG GCCATGGCTG CCCCCATGGC CGGGCTGATG 
GAGAAGGCTG GCAAGGACGG GCCACCCAAG AGCTTCCGGG AGTTGAAAGA GGCCATCAAG 
ATCCTGGAGA GCCTCAAGAA CATGACTGTG GAGCAGCTGC TGACGGGCTC GCCCACCTCT 
CCGACTGTGG AGCCTGAGAA GCCAACTCGG GAGAAGAAGT TTCTGGATGA CATCAAGAAG 
CTACAGGAAA ACCTCAAGAA GACCCTGGAC AATGTGGCCA TTGTAGAGGA GGAGAAGATG 
GAAGCAGTGC CCGACGTAGA GCGCAAGGAG GACAAGCCCG AGGGGCAGTC ACCTGTGAAG 
GCTGAGTGGC CCAGCGAAAC CCCGGTGCTG TGCCAGCAGT GTGGCGGCAA GCCTGGCGTC 
ACCTTCACCA GCGCCAAGGG CGAGGTCTTC TCCGTACTGG AGTTTGCACC CTCAAATCAT 
TCTTTTAAGA AAATTGAGTT CCAGCCTCCA GAAGCCAAGA AGTTCTTCAG CACAGTGCGG 
AAGGAGATGG CGCTGCTGGC TACCTCACTG CCTGAGGGCA TCATGGTCAA GACTTTTGAA 
GATAGAATGG ACCTCTTCTC AGCTCTCATC AAGGGCCCCA CTCGAACCCC CTACGAGGAT 
GGCCTCTACT TGTTTGACAT CCAGCTCCCC AACATCTACC CAGCCGTGCC CCCCCACTTC 
TGCTACCTCT CCCAATGCAG TGGCCGCCTG AACCCCAACC TGTATGACAA TGGGAAGGTG 
TGTGTCAGCC TCCTGGGCAC CTGGATTGGA AAGGGGACAG AGAGGTGGAC AAGCAAGTCC 
AGCCTTCTCC AGGTGCTCAT CTCCATCCAA GGTCTGATCC TGGTAAATGA ACCATACTAC 
AACGAAGCCG GCTTCGACAG TGACCGAGGC CTGCAGGAAG GCTATGAAAA CAGTCGCTGT 
TACAATGAGA TGGCGCTGAT CCGCGTGGTG CAGTCCATGA CCCAGCTGGT GCGGCGGCCC 
CCCGAGGTCT TTGAGCAGGA GATCAGGCAA CACTTTAGCA CTGGTGGCTG GCGGCTGGTG 
AACCGTATCG AGTCCTGGCT GGAAACCCAT GCCCTGCTGG AGAAGGCCCA GGCACTGCCC 
AACGGGGTGC CCAAGGCCAG CAGCTCGCCA GAGCCCCCAG CTGTAGCCGA GCTGTCAGAC 
TCCGGCCAAC AAGAACCTGA GGATGGAGGG CCAGCCCCAG GAGAGGCCTC CCAGGGCTCA 
GACTCAGAGG GCGGTGCCCA GGGCCTGGCC TCAGCTAGCA GGGACCACAC AGACCAGACT 
TCGGAGACCG CACCAGACGC ATCGGTGCCA CCCAGTGTGA AACCAAAGAA GCGGAGAAAG 
AGCTACCGGA GCTTCTTACC TGAGAAGAGT GGCTACCCTG ACATCGGCTT CCCCCTCTTC 
CCACTTTCCA AGGGTTTCAT CAAGAGCATC CGGGGTGTCC TGACGCAGTT CCGGGCTGCC 
CTGCTAGAGG CAGGCATGCC GGAGTGCACA GAGGACAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005257624.1
CDS	50..3931
Translation	MADPAAPTPAAPAPAQAPAPAPEAVPAPAAAPVPAPAPASDSASGPSSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSDSEGEEEGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPAKIAWECPEKNCAQGEGSMAKKVKRLLKKQVVRIMSCSPDTQCSRDHSMEDPDKKGESKTKSEAESASPEETPDGSASPVEMQDEGAEEPHEAGEQLPPFLLKEGRDDRLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGTSRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHIGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAPHKEDEQPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIEEPPIPPLEQPVAPEDKGVVISEEAATAAVQGAVAMAAPMAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIVEEEKMEAVPDVERKEDKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPEGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWLETHALLEKAQALPNGVPKASSSPEPPAVAELSDSGQQEPEDGGPAPGEASQGSDSEGGAQGLASASRDHTDQTSETAPDASVPPSVKPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALLEAGMPECTEDK

Target ORF information:

RefSeq Version	XM_005257567.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005257567.1

ORF Insert Sequence:

ATGGCGGATC CCGCAGCCCC CACGCCCGCA GCTCCCGCTC CAGCCCAGGC CCCGGCTCCA 
GCCCCGGAGG CAGTCCCGGC CCCAGCCGCA GCCCCCGTCC CGGCGCCGGC GCCCGCCTCG 
GACTCGGCCT CCGGGCCGTC CTCGGACTCC GGCCCAGAAG CCGGCTCGCA GCGCCTGCTG 
TTTTCTCACG ACCTGGTGTC GGGCCGTTAC CGTGGCTCCG TGCACTTCGG GCTGGTGCGC 
CTCATCCACG GCGAGGACTC GGACTCGGAG GGCGAGGAGG AGGGCCGCGG GAGCTCGGGG 
TGCTCCGAGG CCGGGGGCGC GGGCCACGAG GAGGGCCGGG CCAGCCCCCT GCGCCGCGGC 
TACGTGCGCG TCCAGTGGTA CCCGGAGGGC GTCAAGCAGC ATGTGAAGGA GACCAAGCTG 
AAACTAGAGG ACCGTTCTGT GGTGCCCCGA GATGTGGTCC GGCACATGCG ATCCACCGAC 
AGTCAGTGTG GCACGGTGAT CGACGTCAAC ATCGACTGTG CCGTCAAGCT CATCGGCACC 
AACTGCATCA TCTATCCCGT CAACAGCAAG GACCTGCAGC ACATCTGGCC CTTCATGTAT 
GGGGACTACA TTGCCTATGA CTGCTGGCTG GGGAAGGTCT ACGACTTGAA GAACCAGATC 
ATCCTGAAGC TATCCAACGG CGCCAGGTGC TCCATGAACA CGGAAGATGG CGCCAAGCTC 
TACGACGTCT GCCCGCACGT CAGCGACTCG GGTCTCTTCT TCGATGATTC CTATGGCTTC 
TACCCAGGCC AGGTGCTCAT TGGCCCTGCC AAGATCTTCT CCAGCGTCCA GTGGCTGTCA 
GGTGTCAAGC CCGTGCTCAG CACCAAGAGC AAGTTCCGAG TGGTGGTGGA AGAGGTGCAG 
GTTGTAGAGT TGAAAGTTAC ATGGATTACC AAGAGTTTCT GTCCAGGGGG CACGGACAGC 
GTCAGCCCCC CACCCTCTGT CATCACCCAG GAAAACCTAG GCAGGGTGAA GCGTCTCGGA 
TGCTTTGACC ATGCTCAGCG GCAGCTTGGG GAGCGCTGTC TGTATGTCTT CCCAGCCAAG 
GTAGAGCCAG CCAAGATTGC CTGGGAATGT CCAGAAAAAA ACTGCGCCCA GGGGGAGGGC 
TCTATGGCCA AGAAGGTGAA GCGCCTGTTG AAGAAGCAGG TTGTGCGGAT CATGTCATGC 
TCCCCAGACA CCCAGTGTTC CCGGGACCAT TCCATGGAAG ACCCAGACAA GAAGGGGGAA 
TCCAAAACCA AGAGCGAAGC GGAGTCTGCC AGCCCTGAGG AGACGCCCGA TGGCTCTGCC 
AGTCCAGTGG AGATGCAGGA CGAGGGTGCA GAGGAGCCCC ACGAGGCAGG AGAGCAGCTG 
CCCCCATTCC TGCTAAAAGA AGGCAGAGAT GACAGGCTGC ACTCGGCAGA GCAGGACGCA 
GATGATGAGG CTGCTGATGA CACGGACGAC ACCAGTTCGG TGACCTCCTC TGCCAGCTCC 
ACCACTTCCT CCCAGAGCGG CAGCGGCACG AGTCGCAAAA AGAGCATCCC CTTGTCCATC 
AAGAACTTAA AGCGCAAACA CAAGAGGAAG AAGAATAAAA TCACTCGAGA CTTCAAGCCA 
GGGGACAGGG TGGCAGTGGA GGTGGTGACC ACGATGACCT CAGCCGACGT GATGTGGCAG 
GATGGCTCCG TGGAATGCAA CATCCGCTCC AACGACCTCT TCCCTGTGCA CCACCTGGAC 
AACAACGAGT TCTGCCCTGG AGACTTCGTG GTAGATAAGC GAGTCCAGAG CTGTCCAGAC 
CCTGCTGTCT ACGGTGTGGT ACAGTCTGGG GACCACATCG GCCGTACCTG CATGGTGAAG 
TGGTTCAAGC TGAGGCCGAG TGGGGACGAC GTGGAGCTGA TTGGAGAAGA GGAAGATGTG 
AGTGTTTACG ACATTGCTGA CCACCCTGAC TTTAGGTTCC GTACAACTGA CATCGTCATC 
CGCATCGGCA ATACTGAGGA TGGGGCTCCT CACAAGGAGG ATGAGCAGCC ATCGGTGGGC 
CAGGTGGCCC GTGTGGACGT CAGCAGCAAG GTGGAGGTGG TGTGGGCTGA CAACTCAAAG 
ACCATCATCC TGCCCCAGCA CTTGTACAAC ATAGAGTCTG AGATTGAGGA GTCAGACTAC 
GATTCGGTAG AAGGCAGCAC CAGCGGGGCA TCCTCGGATG AATGGGAAGA TGATAGTGAC 
AGCTGGGAGA CGGACAATGG GCTGGTGGAG GACGAGCACC CCAAGATAGA GGAGCCCCCC 
ATCCCACCCC TGGAGCAGCC GGTGGCCCCT GAGGACAAGG GAGTGGTGAT CAGTGAAGAG 
GCAGCCACAG CTGCCGTCCA GGGGGCTGTG GCCATGGCTG CCCCCATGGC CGGGCTGATG 
GAGAAGGCTG GCAAGGACGG GCCACCCAAG AGCTTCCGGG AGTTGAAAGA GGCCATCAAG 
ATCCTGGAGA GCCTCAAGAA CATGACTGTG GAGCAGCTGC TGACGGGCTC GCCCACCTCT 
CCGACTGTGG AGCCTGAGAA GCCAACTCGG GAGAAGAAGT TTCTGGATGA CATCAAGAAG 
CTACAGGAAA ACCTCAAGAA GACCCTGGAC AATGTGGCCA TTGTAGAGGA GGAGAAGATG 
GAAGCAGTGC CCGACGTAGA GCGCAAGGAG GACAAGCCCG AGGGGCAGTC ACCTGTGAAG 
GCTGAGTGGC CCAGCGAAAC CCCGGTGCTG TGCCAGCAGT GTGGCGGCAA GCCTGGCGTC 
ACCTTCACCA GCGCCAAGGG CGAGGTCTTC TCCGTACTGG AGTTTGCACC CTCAAATCAT 
TCTTTTAAGA AAATTGAGTT CCAGCCTCCA GAAGCCAAGA AGTTCTTCAG CACAGTGCGG 
AAGGAGATGG CGCTGCTGGC TACCTCACTG CCTGAGGGCA TCATGGTCAA GACTTTTGAA 
GATAGAATGG ACCTCTTCTC AGCTCTCATC AAGGGCCCCA CTCGAACCCC CTACGAGGAT 
GGCCTCTACT TGTTTGACAT CCAGCTCCCC AACATCTACC CAGCCGTGCC CCCCCACTTC 
TGCTACCTCT CCCAATGCAG TGGCCGCCTG AACCCCAACC TGTATGACAA TGGGAAGGTG 
TGTGTCAGCC TCCTGGGCAC CTGGATTGGA AAGGGGACAG AGAGGTGGAC AAGCAAGTCC 
AGCCTTCTCC AGGTGCTCAT CTCCATCCAA GGTCTGATCC TGGTAAATGA ACCATACTAC 
AACGAAGCCG GCTTCGACAG TGACCGAGGC CTGCAGGAAG GCTATGAAAA CAGTCGCTGT 
TACAATGAGA TGGCGCTGAT CCGCGTGGTG CAGTCCATGA CCCAGCTGGT GCGGCGGCCC 
CCCGAGGTCT TTGAGCAGGA GATCAGGCAA CACTTTAGCA CTGGTGGCTG GCGGCTGGTG 
AACCGTATCG AGTCCTGGCT GGAAACCCAT GCCCTGCTGG AGAAGGCCCA GGCACTGCCC 
AACGGGGTGC CCAAGGCCAG CAGCTCGCCA GAGCCCCCAG CTGTAGCCGA GCTGTCAGAC 
TCCGGCCAAC AAGAACCTGA GGATGGAGGG CCAGCCCCAG GAGAGGCCTC CCAGGGCTCA 
GACTCAGAGG GCGGTGCCCA GGGCCTGGCC TCAGCTAGCA GGGACCACAC AGACCAGACT 
TCGGAGACCG CACCAGACGC ATCGGTGCCA CCCAGTGTGA AACCAAAGAA GCGGAGAAAG 
AGCTACCGGA GCTTCTTACC TGAGAAGAGT GGCTACCCTG ACATCGGCTT CCCCCTCTTC 
CCACTTTCCA AGGGTTTCAT CAAGAGCATC CGGGGTGTCC TGACGCAGTT CCGGGCTGCC 
CTGCTAGAGG CAGGCATGCC GGAGTGCACA GAGGACAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2O ( XM_024450865.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306633.1 Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X1, mRNA.

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CloneID	OHu71127
Clone ID Related Accession (Same CDS sequence)	XM_024450865.1
Accession Version	XM_024450865.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATC CCGCAGCCCC CACGCCCGCA GCTCCCGCTC CAGCCCAGGC CCCGGCTCCA 
GCCCCGGAGG CAGTCCCGGC CCCAGCCGCA GCCCCCGTCC CGGCGCCGGC GCCCGCCTCG 
GACTCGGCCT CCGGGCCGTC CTCGGACTCC GGCCCAGAAG CCGGCTCGCA GCGCCTGCTG 
TTTTCTCACG ACCTGGTGTC GGGCCGTTAC CGTGGCTCCG TGCACTTCGG GCTGGTGCGC 
CTCATCCACG GCGAGGACTC GGACTCGGAG GGCGAGGAGG AGGGCCGCGG GAGCTCGGGG 
TGCTCCGAGG CCGGGGGCGC GGGCCACGAG GAGGGCCGGG CCAGCCCCCT GCGCCGCGGC 
TACGTGCGCG TCCAGTGGTA CCCGGAGGGC GTCAAGCAGC ATGTGAAGGA GACCAAGGCA 
CAACAGCAGC AAGCAGCTCC TCTAAATGCA GTGAGCCAGG ACTCAAATAC CAGCGGAAAC 
AGCACCATCA TGGAGACTGG GAGGAGTTTC TGCCCAATTC TGCAGCTGAA ACTAGAGGAC 
CGTTCTGTGG TGCCCCGAGA TGTGGTCCGG CACATGCGAT CCACCGACAG TCAGTGTGGC 
ACGGTGATCG ACGTCAACAT CGACTGTGCC GTCAAGCTCA TCGGCACCAA CTGCATCATC 
TATCCCGTCA ACAGCAAGGA CCTGCAGCAC ATCTGGCCCT TCATGTATGG GGACTACATT 
GCCTATGACT GCTGGCTGGG GAAGGTCTAC GACTTGAAGA ACCAGATCAT CCTGAAGCTA 
TCCAACGGCG CCAGGTGCTC CATGAACACG GAAGATGGCG CCAAGCTCTA CGACGTCTGC 
CCGCACGTCA GCGACTCGGG TCTCTTCTTC GATGATTCCT ATGGCTTCTA CCCAGGCCAG 
GTGCTCATTG GCCCTGCCAA GATCTTCTCC AGCGTCCAGT GGCTGTCAGG TGTCAAGCCC 
GTGCTCAGCA CCAAGAGCAA GTTCCGAGTG GTGGTGGAAG AGGTGCAGGT TGTAGAGTTG 
AAAGTTACAT GGATTACCAA GAGTTTCTGT CCAGGGGGCA CGGACAGCGT CAGCCCCCCA 
CCCTCTGTCA TCACCCAGGA AAACCTAGGC AGGGTGAAGC GTCTCGGATG CTTTGACCAT 
GCTCAGCGGC AGCTTGGGGA GCGCTGTCTG TATGTCTTCC CAGCCAAGGT AGAGCCAGCC 
AAGATTGCCT GGGAATGTCC AGAAAAAAAC TGCGCCCAGG GGGAGGGCTC TATGGCCAAG 
AAGGTGAAGC GCCTGTTGAA GAAGCAGGTT GTGCGGATCA TGTCATGCTC CCCAGACACC 
CAGTGTTCCC GGGACCATTC CATGGAAGAC CCAGACAAGA AGGGGGAATC CAAAACCAAG 
AGCGAAGCGG AGTCTGCCAG CCCTGAGGAG ACGCCCGATG GCTCTGCCAG TCCAGTGGAG 
ATGCAGGACG AGGGTGCAGA GGAGCCCCAC GAGGCAGGAG AGCAGCTGCC CCCATTCCTG 
CTAAAAGAAG GCAGAGATGA CAGGCTGCAC TCGGCAGAGC AGGACGCAGA TGATGAGGCT 
GCTGATGACA CGGACGACAC CAGTTCGGTG ACCTCCTCTG CCAGCTCCAC CACTTCCTCC 
CAGAGCGGCA GCGGCACGAG TCGCAAAAAG AGCATCCCCT TGTCCATCAA GAACTTAAAG 
CGCAAACACA AGAGGAAGAA GAATAAAATC ACTCGAGACT TCAAGCCAGG GGACAGGGTG 
GCAGTGGAGG TGGTGACCAC GATGACCTCA GCCGACGTGA TGTGGCAGGA TGGCTCCGTG 
GAATGCAACA TCCGCTCCAA CGACCTCTTC CCTGTGCACC ACCTGGACAA CAACGAGTTC 
TGCCCTGGAG ACTTCGTGGT AGATAAGCGA GTCCAGAGCT GTCCAGACCC TGCTGTCTAC 
GGTGTGGTAC AGTCTGGGGA CCACATCGGC CGTACCTGCA TGGTGAAGTG GTTCAAGCTG 
AGGCCGAGTG GGGACGACGT GGAGCTGATT GGAGAAGAGG AAGATGTGAG TGTTTACGAC 
ATTGCTGACC ACCCTGACTT TAGGTTCCGT ACAACTGACA TCGTCATCCG CATCGGCAAT 
ACTGAGGATG GGGCTCCTCA CAAGGAGGAT GAGCAGCCAT CGGTGGGCCA GGTGGCCCGT 
GTGGACGTCA GCAGCAAGGT GGAGGTGGTG TGGGCTGACA ACTCAAAGAC CATCATCCTG 
CCCCAGCACT TGTACAACAT AGAGTCTGAG ATTGAGGAGT CAGACTACGA TTCGGTAGAA 
GGCAGCACCA GCGGGGCATC CTCGGATGAA TGGGAAGATG ATAGTGACAG CTGGGAGACG 
GACAATGGGC TGGTGGAGGA CGAGCACCCC AAGATAGAGG AGCCCCCCAT CCCACCCCTG 
GAGCAGCCGG TGGCCCCTGA GGACAAGGGA GTGGTGATCA GTGAAGAGGC AGCCACAGCT 
GCCGTCCAGG GGGCTGTGGC CATGGCTGCC CCCATGGCCG GGCTGATGGA GAAGGCTGGC 
AAGGACGGGC CACCCAAGAG CTTCCGGGAG TTGAAAGAGG CCATCAAGAT CCTGGAGAGC 
CTCAAGAACA TGACTGTGGA GCAGCTGCTG ACGGGCTCGC CCACCTCTCC GACTGTGGAG 
CCTGAGAAGC CAACTCGGGA GAAGAAGTTT CTGGATGACA TCAAGAAGCT ACAGGAAAAC 
CTCAAGAAGA CCCTGGACAA TGTGGCCATT GTAGAGGAGG AGAAGATGGA AGCAGTGCCC 
GACGTAGAGC GCAAGGAGGA CAAGCCCGAG GGGCAGTCAC CTGTGAAGGC TGAGTGGCCC 
AGCGAAACCC CGGTGCTGTG CCAGCAGTGT GGCGGCAAGC CTGGCGTCAC CTTCACCAGC 
GCCAAGGGCG AGGTCTTCTC CGTACTGGAG TTTGCACCCT CAAATCATTC TTTTAAGAAA 
ATTGAGTTCC AGCCTCCAGA AGCCAAGAAG TTCTTCAGCA CAGTGCGGAA GGAGATGGCG 
CTGCTGGCTA CCTCACTGCC TGAGGGCATC ATGGTCAAGA CTTTTGAAGA TAGAATGGAC 
CTCTTCTCAG CTCTCATCAA GGGCCCCACT CGAACCCCCT ACGAGGATGG CCTCTACTTG 
TTTGACATCC AGCTCCCCAA CATCTACCCA GCCGTGCCCC CCCACTTCTG CTACCTCTCC 
CAATGCAGTG GCCGCCTGAA CCCCAACCTG TATGACAATG GGAAGGTGTG TGTCAGCCTC 
CTGGGCACCT GGATTGGAAA GGGGACAGAG AGGTGGACAA GCAAGTCCAG CCTTCTCCAG 
GTGCTCATCT CCATCCAAGG TCTGATCCTG GTAAATGAAC CATACTACAA CGAAGCCGGC 
TTCGACAGTG ACCGAGGCCT GCAGGAAGGC TATGAAAACA GTCGCTGTTA CAATGAGATG 
GCGCTGATCC GCGTGGTGCA GTCCATGACC CAGCTGGTGC GGCGGCCCCC CGAGGTCTTT 
GAGCAGGAGA TCAGGCAACA CTTTAGCACT GGTGGCTGGC GGCTGGTGAA CCGTATCGAG 
TCCTGGCTGG AAACCCATGC CCTGCTGGAG AAGGCCCAGG CACTGCCCAA CGGGGTGCCC 
AAGGCCAGCA GCTCGCCAGA GCCCCCAGCT GTAGCCGAGC TGTCAGACTC CGGCCAACAA 
GAACCTGAGG ATGGAGGGCC AGCCCCAGGA GAGGCCTCCC AGGGCTCAGA CTCAGAGGGC 
GGTGCCCAGG GCCTGGCCTC AGCTAGCAGG GACCACACAG ACCAGACTTC GGAGACCGCA 
CCAGACGCAT CGGTGCCACC CAGTGTGAAA CCAAAGAAGC GGAGAAAGAG CTACCGGAGC 
TTCTTACCTG AGAAGAGTGG CTACCCTGAC ATCGGCTTCC CCCTCTTCCC ACTTTCCAAG 
GGTTTCATCA AGAGCATCCG GGGTGTCCTG ACGCAGTTCC GGGCTGCCCT GCTAGAGGCA 
GGCATGCCGG AGTGCACAGA GGACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306633.1
CDS	94..4083
Translation	MADPAAPTPAAPAPAQAPAPAPEAVPAPAAAPVPAPAPASDSASGPSSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSDSEGEEEGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKAQQQQAAPLNAVSQDSNTSGNSTIMETGRSFCPILQLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPAKIAWECPEKNCAQGEGSMAKKVKRLLKKQVVRIMSCSPDTQCSRDHSMEDPDKKGESKTKSEAESASPEETPDGSASPVEMQDEGAEEPHEAGEQLPPFLLKEGRDDRLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGTSRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHIGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAPHKEDEQPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIEEPPIPPLEQPVAPEDKGVVISEEAATAAVQGAVAMAAPMAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIVEEEKMEAVPDVERKEDKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPEGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWLETHALLEKAQALPNGVPKASSSPEPPAVAELSDSGQQEPEDGGPAPGEASQGSDSEGGAQGLASASRDHTDQTSETAPDASVPPSVKPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALLEAGMPECTEDK

Target ORF information:

RefSeq Version	XM_024450865.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024450865.1

ORF Insert Sequence:

ATGGCGGATC CCGCAGCCCC CACGCCCGCA GCTCCCGCTC CAGCCCAGGC CCCGGCTCCA 
GCCCCGGAGG CAGTCCCGGC CCCAGCCGCA GCCCCCGTCC CGGCGCCGGC GCCCGCCTCG 
GACTCGGCCT CCGGGCCGTC CTCGGACTCC GGCCCAGAAG CCGGCTCGCA GCGCCTGCTG 
TTTTCTCACG ACCTGGTGTC GGGCCGTTAC CGTGGCTCCG TGCACTTCGG GCTGGTGCGC 
CTCATCCACG GCGAGGACTC GGACTCGGAG GGCGAGGAGG AGGGCCGCGG GAGCTCGGGG 
TGCTCCGAGG CCGGGGGCGC GGGCCACGAG GAGGGCCGGG CCAGCCCCCT GCGCCGCGGC 
TACGTGCGCG TCCAGTGGTA CCCGGAGGGC GTCAAGCAGC ATGTGAAGGA GACCAAGGCA 
CAACAGCAGC AAGCAGCTCC TCTAAATGCA GTGAGCCAGG ACTCAAATAC CAGCGGAAAC 
AGCACCATCA TGGAGACTGG GAGGAGTTTC TGCCCAATTC TGCAGCTGAA ACTAGAGGAC 
CGTTCTGTGG TGCCCCGAGA TGTGGTCCGG CACATGCGAT CCACCGACAG TCAGTGTGGC 
ACGGTGATCG ACGTCAACAT CGACTGTGCC GTCAAGCTCA TCGGCACCAA CTGCATCATC 
TATCCCGTCA ACAGCAAGGA CCTGCAGCAC ATCTGGCCCT TCATGTATGG GGACTACATT 
GCCTATGACT GCTGGCTGGG GAAGGTCTAC GACTTGAAGA ACCAGATCAT CCTGAAGCTA 
TCCAACGGCG CCAGGTGCTC CATGAACACG GAAGATGGCG CCAAGCTCTA CGACGTCTGC 
CCGCACGTCA GCGACTCGGG TCTCTTCTTC GATGATTCCT ATGGCTTCTA CCCAGGCCAG 
GTGCTCATTG GCCCTGCCAA GATCTTCTCC AGCGTCCAGT GGCTGTCAGG TGTCAAGCCC 
GTGCTCAGCA CCAAGAGCAA GTTCCGAGTG GTGGTGGAAG AGGTGCAGGT TGTAGAGTTG 
AAAGTTACAT GGATTACCAA GAGTTTCTGT CCAGGGGGCA CGGACAGCGT CAGCCCCCCA 
CCCTCTGTCA TCACCCAGGA AAACCTAGGC AGGGTGAAGC GTCTCGGATG CTTTGACCAT 
GCTCAGCGGC AGCTTGGGGA GCGCTGTCTG TATGTCTTCC CAGCCAAGGT AGAGCCAGCC 
AAGATTGCCT GGGAATGTCC AGAAAAAAAC TGCGCCCAGG GGGAGGGCTC TATGGCCAAG 
AAGGTGAAGC GCCTGTTGAA GAAGCAGGTT GTGCGGATCA TGTCATGCTC CCCAGACACC 
CAGTGTTCCC GGGACCATTC CATGGAAGAC CCAGACAAGA AGGGGGAATC CAAAACCAAG 
AGCGAAGCGG AGTCTGCCAG CCCTGAGGAG ACGCCCGATG GCTCTGCCAG TCCAGTGGAG 
ATGCAGGACG AGGGTGCAGA GGAGCCCCAC GAGGCAGGAG AGCAGCTGCC CCCATTCCTG 
CTAAAAGAAG GCAGAGATGA CAGGCTGCAC TCGGCAGAGC AGGACGCAGA TGATGAGGCT 
GCTGATGACA CGGACGACAC CAGTTCGGTG ACCTCCTCTG CCAGCTCCAC CACTTCCTCC 
CAGAGCGGCA GCGGCACGAG TCGCAAAAAG AGCATCCCCT TGTCCATCAA GAACTTAAAG 
CGCAAACACA AGAGGAAGAA GAATAAAATC ACTCGAGACT TCAAGCCAGG GGACAGGGTG 
GCAGTGGAGG TGGTGACCAC GATGACCTCA GCCGACGTGA TGTGGCAGGA TGGCTCCGTG 
GAATGCAACA TCCGCTCCAA CGACCTCTTC CCTGTGCACC ACCTGGACAA CAACGAGTTC 
TGCCCTGGAG ACTTCGTGGT AGATAAGCGA GTCCAGAGCT GTCCAGACCC TGCTGTCTAC 
GGTGTGGTAC AGTCTGGGGA CCACATCGGC CGTACCTGCA TGGTGAAGTG GTTCAAGCTG 
AGGCCGAGTG GGGACGACGT GGAGCTGATT GGAGAAGAGG AAGATGTGAG TGTTTACGAC 
ATTGCTGACC ACCCTGACTT TAGGTTCCGT ACAACTGACA TCGTCATCCG CATCGGCAAT 
ACTGAGGATG GGGCTCCTCA CAAGGAGGAT GAGCAGCCAT CGGTGGGCCA GGTGGCCCGT 
GTGGACGTCA GCAGCAAGGT GGAGGTGGTG TGGGCTGACA ACTCAAAGAC CATCATCCTG 
CCCCAGCACT TGTACAACAT AGAGTCTGAG ATTGAGGAGT CAGACTACGA TTCGGTAGAA 
GGCAGCACCA GCGGGGCATC CTCGGATGAA TGGGAAGATG ATAGTGACAG CTGGGAGACG 
GACAATGGGC TGGTGGAGGA CGAGCACCCC AAGATAGAGG AGCCCCCCAT CCCACCCCTG 
GAGCAGCCGG TGGCCCCTGA GGACAAGGGA GTGGTGATCA GTGAAGAGGC AGCCACAGCT 
GCCGTCCAGG GGGCTGTGGC CATGGCTGCC CCCATGGCCG GGCTGATGGA GAAGGCTGGC 
AAGGACGGGC CACCCAAGAG CTTCCGGGAG TTGAAAGAGG CCATCAAGAT CCTGGAGAGC 
CTCAAGAACA TGACTGTGGA GCAGCTGCTG ACGGGCTCGC CCACCTCTCC GACTGTGGAG 
CCTGAGAAGC CAACTCGGGA GAAGAAGTTT CTGGATGACA TCAAGAAGCT ACAGGAAAAC 
CTCAAGAAGA CCCTGGACAA TGTGGCCATT GTAGAGGAGG AGAAGATGGA AGCAGTGCCC 
GACGTAGAGC GCAAGGAGGA CAAGCCCGAG GGGCAGTCAC CTGTGAAGGC TGAGTGGCCC 
AGCGAAACCC CGGTGCTGTG CCAGCAGTGT GGCGGCAAGC CTGGCGTCAC CTTCACCAGC 
GCCAAGGGCG AGGTCTTCTC CGTACTGGAG TTTGCACCCT CAAATCATTC TTTTAAGAAA 
ATTGAGTTCC AGCCTCCAGA AGCCAAGAAG TTCTTCAGCA CAGTGCGGAA GGAGATGGCG 
CTGCTGGCTA CCTCACTGCC TGAGGGCATC ATGGTCAAGA CTTTTGAAGA TAGAATGGAC 
CTCTTCTCAG CTCTCATCAA GGGCCCCACT CGAACCCCCT ACGAGGATGG CCTCTACTTG 
TTTGACATCC AGCTCCCCAA CATCTACCCA GCCGTGCCCC CCCACTTCTG CTACCTCTCC 
CAATGCAGTG GCCGCCTGAA CCCCAACCTG TATGACAATG GGAAGGTGTG TGTCAGCCTC 
CTGGGCACCT GGATTGGAAA GGGGACAGAG AGGTGGACAA GCAAGTCCAG CCTTCTCCAG 
GTGCTCATCT CCATCCAAGG TCTGATCCTG GTAAATGAAC CATACTACAA CGAAGCCGGC 
TTCGACAGTG ACCGAGGCCT GCAGGAAGGC TATGAAAACA GTCGCTGTTA CAATGAGATG 
GCGCTGATCC GCGTGGTGCA GTCCATGACC CAGCTGGTGC GGCGGCCCCC CGAGGTCTTT 
GAGCAGGAGA TCAGGCAACA CTTTAGCACT GGTGGCTGGC GGCTGGTGAA CCGTATCGAG 
TCCTGGCTGG AAACCCATGC CCTGCTGGAG AAGGCCCAGG CACTGCCCAA CGGGGTGCCC 
AAGGCCAGCA GCTCGCCAGA GCCCCCAGCT GTAGCCGAGC TGTCAGACTC CGGCCAACAA 
GAACCTGAGG ATGGAGGGCC AGCCCCAGGA GAGGCCTCCC AGGGCTCAGA CTCAGAGGGC 
GGTGCCCAGG GCCTGGCCTC AGCTAGCAGG GACCACACAG ACCAGACTTC GGAGACCGCA 
CCAGACGCAT CGGTGCCACC CAGTGTGAAA CCAAAGAAGC GGAGAAAGAG CTACCGGAGC 
TTCTTACCTG AGAAGAGTGG CTACCCTGAC ATCGGCTTCC CCCTCTTCCC ACTTTCCAAG 
GGTTTCATCA AGAGCATCCG GGGTGTCCTG ACGCAGTTCC GGGCTGCCCT GCTAGAGGCA 
GGCATGCCGG AGTGCACAGA GGACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2O ( XM_024450866.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306634.1 Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu71128
Clone ID Related Accession (Same CDS sequence)	XM_024450866.1
Accession Version	XM_024450866.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATC CCGCAGCCCC CACGCCCGCA GCTCCCGCTC CAGCCCAGGC CCCGGCTCCA 
GCCCCGGAGG CAGTCCCGGC CCCAGCCGCA GCCCCCGTCC CGGCGCCGGC GCCCGCCTCG 
GACTCGGCCT CCGGGCCGTC CTCGGACTCC GGCCCAGAAG CCGGCTCGCA GCGCCTGCTG 
TTTTCTCACG ACCTGGTGTC GGGCCGTTAC CGTGGCTCCG TGCACTTCGG GCTGGTGCGC 
CTCATCCACG GCGAGGACTC GGACTCGGAG GGCGAGGAGG AGGGCCGCGG GAGCTCGGGG 
TGCTCCGAGG CCGGGGGCGC GGGCCACGAG GAGGGCCGGG CCAGCCCCCT GCGCCGCGGC 
TACGTGCGCG TCCAGTGGTA CCCGGAGGGC GTCAAGCAGC ATGTGAAGGA GACCAAGGCA 
CAACAGCAGC AAGCAGCTCC TCTAAATGCA GTGAGCCAGG ACTCAAATAC CAGCGGAAAC 
AGCACCATCA TGGAGACTGG GAGGAGTTTC TGCCCAATTC TGCAGCTGAA ACTAGAGGAC 
CGTTCTGTGG TGCCCCGAGA TGTGGTCCGG CACATGCGAT CCACCGACAG TCAGTGTGGC 
ACGGTGATCG ACGTCAACAT CGACTGTGCC GTCAAGCTCA TCGGCACCAA CTGCATCATC 
TATCCCGTCA ACAGCAAGGA CCTGCAGCAC ATCTGGCCCT TCATGTATGG GGACTACATT 
GCCTATGACT GCTGGCTGGG GAAGGTCTAC GACTTGAAGA ACCAGATCAT CCTGAAGCTA 
TCCAACGGCG CCAGGTGCTC CATGAACACG GAAGATGGCG CCAAGCTCTA CGACGTCTGC 
CCGCACGTCA GCGACTCGGG TCTCTTCTTC GATGATTCCT ATGGCTTCTA CCCAGGCCAG 
GTGCTCATTG GCCCTGCCAA GATCTTCTCC AGCGTCCAGT GGCTGTCAGG TGTCAAGCCC 
GTGCTCAGCA CCAAGAGCAA GTTCCGAGTG GTGGTGGAAG AGGTGCAGGT TGTAGAGTTG 
AAAGTTACAT GGATTACCAA GAGTTTCTGT CCAGGGGGCA CGGACAGCGT CAGCCCCCCA 
CCCTCTGTCA TCACCCAGGA AAACCTAGGC AGGGTGAAGC GTCTCGGATG CTTTGACCAT 
GCTCAGCGGC AGCTTGGGGA GCGCTGTCTG TATGTCTTCC CAGCCAAGGT AGAGCCAGCC 
AAGATTGCCT GGGAATGTCC AGAAAAAAAC TGCGCCCAGG GGGAGGGCTC TATGGCCAAG 
AAGGTGAAGC GCCTGTTGAA GAAGCAGGTT GTGCGGATCA TGTCATGCTC CCCAGACACC 
CAGTGTTCCC GGGACCATTC CATGGAAGAC CCAGACAAGA AGGGGGAATC CAAAACCAAG 
AGCGAAGCGG AGTCTGCCAG CCCTGAGGAG ACGCCCGATG GCTCTGCCAG TCCAGTGGAG 
ATGCAGGACG AGGGTGCAGA GGAGCCCCAC GAGGCAGGAG AGCAGCTGCC CCCATTCCTG 
CTAAAAGAAG GCAGAGATGA CAGGCTGCAC TCGGCAGAGC AGGACGCAGA TGATGAGGCT 
GCTGATGACA CGGACGACAC CAGTTCGGTG ACCTCCTCTG CCAGCTCCAC CACTTCCTCC 
CAGAGCGGCA GCGGCACGAG TCGCAAAAAG AGCATCCCCT TGTCCATCAA GAACTTAAAG 
CGCAAACACA AGAGGAAGAA GAATAAAATC ACTCGAGACT TCAAGCCAGG GGACAGGGTG 
GCAGTGGAGG TGGTGACCAC GATGACCTCA GCCGACGTGA TGTGGCAGGA TGGCTCCGTG 
GAATGCAACA TCCGCTCCAA CGACCTCTTC CCTGTGCACC ACCTGGACAA CAACGAGTTC 
TGCCCTGGAG ACTTCGTGGT AGATAAGCGA GTCCAGAGCT GTCCAGACCC TGCTGTCTAC 
GGTGTGGTAC AGTCTGGGGA CCACATCGGC CGTACCTGCA TGGTGAAGTG GTTCAAGCTG 
AGGCCGAGTG GGGACGACGT GGAGCTGATT GGAGAAGAGG AAGATGTGAG TGTTTACGAC 
ATTGCTGACC ACCCTGACTT TAGGTTCCGT ACAACTGACA TCGTCATCCG CATCGGCAAT 
ACTGAGGATG GGGCTCCTCA CAAGGAGGAT GAGCCATCGG TGGGCCAGGT GGCCCGTGTG 
GACGTCAGCA GCAAGGTGGA GGTGGTGTGG GCTGACAACT CAAAGACCAT CATCCTGCCC 
CAGCACTTGT ACAACATAGA GTCTGAGATT GAGGAGTCAG ACTACGATTC GGTAGAAGGC 
AGCACCAGCG GGGCATCCTC GGATGAATGG GAAGATGATA GTGACAGCTG GGAGACGGAC 
AATGGGCTGG TGGAGGACGA GCACCCCAAG ATAGAGGAGC CCCCCATCCC ACCCCTGGAG 
CAGCCGGTGG CCCCTGAGGA CAAGGGAGTG GTGATCAGTG AAGAGGCAGC CACAGCTGCC 
GTCCAGGGGG CTGTGGCCAT GGCTGCCCCC ATGGCCGGGC TGATGGAGAA GGCTGGCAAG 
GACGGGCCAC CCAAGAGCTT CCGGGAGTTG AAAGAGGCCA TCAAGATCCT GGAGAGCCTC 
AAGAACATGA CTGTGGAGCA GCTGCTGACG GGCTCGCCCA CCTCTCCGAC TGTGGAGCCT 
GAGAAGCCAA CTCGGGAGAA GAAGTTTCTG GATGACATCA AGAAGCTACA GGAAAACCTC 
AAGAAGACCC TGGACAATGT GGCCATTGTA GAGGAGGAGA AGATGGAAGC AGTGCCCGAC 
GTAGAGCGCA AGGAGGACAA GCCCGAGGGG CAGTCACCTG TGAAGGCTGA GTGGCCCAGC 
GAAACCCCGG TGCTGTGCCA GCAGTGTGGC GGCAAGCCTG GCGTCACCTT CACCAGCGCC 
AAGGGCGAGG TCTTCTCCGT ACTGGAGTTT GCACCCTCAA ATCATTCTTT TAAGAAAATT 
GAGTTCCAGC CTCCAGAAGC CAAGAAGTTC TTCAGCACAG TGCGGAAGGA GATGGCGCTG 
CTGGCTACCT CACTGCCTGA GGGCATCATG GTCAAGACTT TTGAAGATAG AATGGACCTC 
TTCTCAGCTC TCATCAAGGG CCCCACTCGA ACCCCCTACG AGGATGGCCT CTACTTGTTT 
GACATCCAGC TCCCCAACAT CTACCCAGCC GTGCCCCCCC ACTTCTGCTA CCTCTCCCAA 
TGCAGTGGCC GCCTGAACCC CAACCTGTAT GACAATGGGA AGGTGTGTGT CAGCCTCCTG 
GGCACCTGGA TTGGAAAGGG GACAGAGAGG TGGACAAGCA AGTCCAGCCT TCTCCAGGTG 
CTCATCTCCA TCCAAGGTCT GATCCTGGTA AATGAACCAT ACTACAACGA AGCCGGCTTC 
GACAGTGACC GAGGCCTGCA GGAAGGCTAT GAAAACAGTC GCTGTTACAA TGAGATGGCG 
CTGATCCGCG TGGTGCAGTC CATGACCCAG CTGGTGCGGC GGCCCCCCGA GGTCTTTGAG 
CAGGAGATCA GGCAACACTT TAGCACTGGT GGCTGGCGGC TGGTGAACCG TATCGAGTCC 
TGGCTGGAAA CCCATGCCCT GCTGGAGAAG GCCCAGGCAC TGCCCAACGG GGTGCCCAAG 
GCCAGCAGCT CGCCAGAGCC CCCAGCTGTA GCCGAGCTGT CAGACTCCGG CCAACAAGAA 
CCTGAGGATG GAGGGCCAGC CCCAGGAGAG GCCTCCCAGG GCTCAGACTC AGAGGGCGGT 
GCCCAGGGCC TGGCCTCAGC TAGCAGGGAC CACACAGACC AGACTTCGGA GACCGCACCA 
GACGCATCGG TGCCACCCAG TGTGAAACCA AAGAAGCGGA GAAAGAGCTA CCGGAGCTTC 
TTACCTGAGA AGAGTGGCTA CCCTGACATC GGCTTCCCCC TCTTCCCACT TTCCAAGGGT 
TTCATCAAGA GCATCCGGGG TGTCCTGACG CAGTTCCGGG CTGCCCTGCT AGAGGCAGGC 
ATGCCGGAGT GCACAGAGGA CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306634.1
CDS	94..4080
Translation	MADPAAPTPAAPAPAQAPAPAPEAVPAPAAAPVPAPAPASDSASGPSSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSDSEGEEEGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKAQQQQAAPLNAVSQDSNTSGNSTIMETGRSFCPILQLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPAKIAWECPEKNCAQGEGSMAKKVKRLLKKQVVRIMSCSPDTQCSRDHSMEDPDKKGESKTKSEAESASPEETPDGSASPVEMQDEGAEEPHEAGEQLPPFLLKEGRDDRLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGTSRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHIGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAPHKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIEEPPIPPLEQPVAPEDKGVVISEEAATAAVQGAVAMAAPMAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIVEEEKMEAVPDVERKEDKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPEGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWLETHALLEKAQALPNGVPKASSSPEPPAVAELSDSGQQEPEDGGPAPGEASQGSDSEGGAQGLASASRDHTDQTSETAPDASVPPSVKPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALLEAGMPECTEDK

Target ORF information:

RefSeq Version	XM_024450866.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024450866.1

ORF Insert Sequence:

ATGGCGGATC CCGCAGCCCC CACGCCCGCA GCTCCCGCTC CAGCCCAGGC CCCGGCTCCA 
GCCCCGGAGG CAGTCCCGGC CCCAGCCGCA GCCCCCGTCC CGGCGCCGGC GCCCGCCTCG 
GACTCGGCCT CCGGGCCGTC CTCGGACTCC GGCCCAGAAG CCGGCTCGCA GCGCCTGCTG 
TTTTCTCACG ACCTGGTGTC GGGCCGTTAC CGTGGCTCCG TGCACTTCGG GCTGGTGCGC 
CTCATCCACG GCGAGGACTC GGACTCGGAG GGCGAGGAGG AGGGCCGCGG GAGCTCGGGG 
TGCTCCGAGG CCGGGGGCGC GGGCCACGAG GAGGGCCGGG CCAGCCCCCT GCGCCGCGGC 
TACGTGCGCG TCCAGTGGTA CCCGGAGGGC GTCAAGCAGC ATGTGAAGGA GACCAAGGCA 
CAACAGCAGC AAGCAGCTCC TCTAAATGCA GTGAGCCAGG ACTCAAATAC CAGCGGAAAC 
AGCACCATCA TGGAGACTGG GAGGAGTTTC TGCCCAATTC TGCAGCTGAA ACTAGAGGAC 
CGTTCTGTGG TGCCCCGAGA TGTGGTCCGG CACATGCGAT CCACCGACAG TCAGTGTGGC 
ACGGTGATCG ACGTCAACAT CGACTGTGCC GTCAAGCTCA TCGGCACCAA CTGCATCATC 
TATCCCGTCA ACAGCAAGGA CCTGCAGCAC ATCTGGCCCT TCATGTATGG GGACTACATT 
GCCTATGACT GCTGGCTGGG GAAGGTCTAC GACTTGAAGA ACCAGATCAT CCTGAAGCTA 
TCCAACGGCG CCAGGTGCTC CATGAACACG GAAGATGGCG CCAAGCTCTA CGACGTCTGC 
CCGCACGTCA GCGACTCGGG TCTCTTCTTC GATGATTCCT ATGGCTTCTA CCCAGGCCAG 
GTGCTCATTG GCCCTGCCAA GATCTTCTCC AGCGTCCAGT GGCTGTCAGG TGTCAAGCCC 
GTGCTCAGCA CCAAGAGCAA GTTCCGAGTG GTGGTGGAAG AGGTGCAGGT TGTAGAGTTG 
AAAGTTACAT GGATTACCAA GAGTTTCTGT CCAGGGGGCA CGGACAGCGT CAGCCCCCCA 
CCCTCTGTCA TCACCCAGGA AAACCTAGGC AGGGTGAAGC GTCTCGGATG CTTTGACCAT 
GCTCAGCGGC AGCTTGGGGA GCGCTGTCTG TATGTCTTCC CAGCCAAGGT AGAGCCAGCC 
AAGATTGCCT GGGAATGTCC AGAAAAAAAC TGCGCCCAGG GGGAGGGCTC TATGGCCAAG 
AAGGTGAAGC GCCTGTTGAA GAAGCAGGTT GTGCGGATCA TGTCATGCTC CCCAGACACC 
CAGTGTTCCC GGGACCATTC CATGGAAGAC CCAGACAAGA AGGGGGAATC CAAAACCAAG 
AGCGAAGCGG AGTCTGCCAG CCCTGAGGAG ACGCCCGATG GCTCTGCCAG TCCAGTGGAG 
ATGCAGGACG AGGGTGCAGA GGAGCCCCAC GAGGCAGGAG AGCAGCTGCC CCCATTCCTG 
CTAAAAGAAG GCAGAGATGA CAGGCTGCAC TCGGCAGAGC AGGACGCAGA TGATGAGGCT 
GCTGATGACA CGGACGACAC CAGTTCGGTG ACCTCCTCTG CCAGCTCCAC CACTTCCTCC 
CAGAGCGGCA GCGGCACGAG TCGCAAAAAG AGCATCCCCT TGTCCATCAA GAACTTAAAG 
CGCAAACACA AGAGGAAGAA GAATAAAATC ACTCGAGACT TCAAGCCAGG GGACAGGGTG 
GCAGTGGAGG TGGTGACCAC GATGACCTCA GCCGACGTGA TGTGGCAGGA TGGCTCCGTG 
GAATGCAACA TCCGCTCCAA CGACCTCTTC CCTGTGCACC ACCTGGACAA CAACGAGTTC 
TGCCCTGGAG ACTTCGTGGT AGATAAGCGA GTCCAGAGCT GTCCAGACCC TGCTGTCTAC 
GGTGTGGTAC AGTCTGGGGA CCACATCGGC CGTACCTGCA TGGTGAAGTG GTTCAAGCTG 
AGGCCGAGTG GGGACGACGT GGAGCTGATT GGAGAAGAGG AAGATGTGAG TGTTTACGAC 
ATTGCTGACC ACCCTGACTT TAGGTTCCGT ACAACTGACA TCGTCATCCG CATCGGCAAT 
ACTGAGGATG GGGCTCCTCA CAAGGAGGAT GAGCCATCGG TGGGCCAGGT GGCCCGTGTG 
GACGTCAGCA GCAAGGTGGA GGTGGTGTGG GCTGACAACT CAAAGACCAT CATCCTGCCC 
CAGCACTTGT ACAACATAGA GTCTGAGATT GAGGAGTCAG ACTACGATTC GGTAGAAGGC 
AGCACCAGCG GGGCATCCTC GGATGAATGG GAAGATGATA GTGACAGCTG GGAGACGGAC 
AATGGGCTGG TGGAGGACGA GCACCCCAAG ATAGAGGAGC CCCCCATCCC ACCCCTGGAG 
CAGCCGGTGG CCCCTGAGGA CAAGGGAGTG GTGATCAGTG AAGAGGCAGC CACAGCTGCC 
GTCCAGGGGG CTGTGGCCAT GGCTGCCCCC ATGGCCGGGC TGATGGAGAA GGCTGGCAAG 
GACGGGCCAC CCAAGAGCTT CCGGGAGTTG AAAGAGGCCA TCAAGATCCT GGAGAGCCTC 
AAGAACATGA CTGTGGAGCA GCTGCTGACG GGCTCGCCCA CCTCTCCGAC TGTGGAGCCT 
GAGAAGCCAA CTCGGGAGAA GAAGTTTCTG GATGACATCA AGAAGCTACA GGAAAACCTC 
AAGAAGACCC TGGACAATGT GGCCATTGTA GAGGAGGAGA AGATGGAAGC AGTGCCCGAC 
GTAGAGCGCA AGGAGGACAA GCCCGAGGGG CAGTCACCTG TGAAGGCTGA GTGGCCCAGC 
GAAACCCCGG TGCTGTGCCA GCAGTGTGGC GGCAAGCCTG GCGTCACCTT CACCAGCGCC 
AAGGGCGAGG TCTTCTCCGT ACTGGAGTTT GCACCCTCAA ATCATTCTTT TAAGAAAATT 
GAGTTCCAGC CTCCAGAAGC CAAGAAGTTC TTCAGCACAG TGCGGAAGGA GATGGCGCTG 
CTGGCTACCT CACTGCCTGA GGGCATCATG GTCAAGACTT TTGAAGATAG AATGGACCTC 
TTCTCAGCTC TCATCAAGGG CCCCACTCGA ACCCCCTACG AGGATGGCCT CTACTTGTTT 
GACATCCAGC TCCCCAACAT CTACCCAGCC GTGCCCCCCC ACTTCTGCTA CCTCTCCCAA 
TGCAGTGGCC GCCTGAACCC CAACCTGTAT GACAATGGGA AGGTGTGTGT CAGCCTCCTG 
GGCACCTGGA TTGGAAAGGG GACAGAGAGG TGGACAAGCA AGTCCAGCCT TCTCCAGGTG 
CTCATCTCCA TCCAAGGTCT GATCCTGGTA AATGAACCAT ACTACAACGA AGCCGGCTTC 
GACAGTGACC GAGGCCTGCA GGAAGGCTAT GAAAACAGTC GCTGTTACAA TGAGATGGCG 
CTGATCCGCG TGGTGCAGTC CATGACCCAG CTGGTGCGGC GGCCCCCCGA GGTCTTTGAG 
CAGGAGATCA GGCAACACTT TAGCACTGGT GGCTGGCGGC TGGTGAACCG TATCGAGTCC 
TGGCTGGAAA CCCATGCCCT GCTGGAGAAG GCCCAGGCAC TGCCCAACGG GGTGCCCAAG 
GCCAGCAGCT CGCCAGAGCC CCCAGCTGTA GCCGAGCTGT CAGACTCCGG CCAACAAGAA 
CCTGAGGATG GAGGGCCAGC CCCAGGAGAG GCCTCCCAGG GCTCAGACTC AGAGGGCGGT 
GCCCAGGGCC TGGCCTCAGC TAGCAGGGAC CACACAGACC AGACTTCGGA GACCGCACCA 
GACGCATCGG TGCCACCCAG TGTGAAACCA AAGAAGCGGA GAAAGAGCTA CCGGAGCTTC 
TTACCTGAGA AGAGTGGCTA CCCTGACATC GGCTTCCCCC TCTTCCCACT TTCCAAGGGT 
TTCATCAAGA GCATCCGGGG TGTCCTGACG CAGTTCCGGG CTGCCCTGCT AGAGGCAGGC 
ATGCCGGAGT GCACAGAGGA CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2O ( XM_024450867.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306635.1 Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu71129
Clone ID Related Accession (Same CDS sequence)	XM_024450867.1
Accession Version	XM_024450867.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATC CCGCAGCCCC CACGCCCGCA GCTCCCGCTC CAGCCCAGGC CCCGGCTCCA 
GCCCCGGAGG CAGTCCCGGC CCCAGCCGCA GCCCCCGTCC CGGCGCCGGC GCCCGCCTCG 
GACTCGGCCT CCGGGCCGTC CTCGGACTCC GGCCCAGAAG CCGGCTCGCA GCGCCTGCTG 
TTTTCTCACG ACCTGGTGTC GGGCCGTTAC CGTGGCTCCG TGCACTTCGG GCTGGTGCGC 
CTCATCCACG GCGAGGACTC GGACTCGGAG GGCGAGGAGG AGGGCCGCGG GAGCTCGGGG 
TGCTCCGAGG CCGGGGGCGC GGGCCACGAG GAGGGCCGGG CCAGCCCCCT GCGCCGCGGC 
TACGTGCGCG TCCAGTGGTA CCCGGAGGGC GTCAAGCAGC ATGTGAAGGA GACCAAGGCA 
CAACAGCAGC AAGCAGCTCC TCTAAATGCA GTGAGCCAGG ACTCAAATAC CAGCGGAAAC 
AGCACCATCA TGGAGACTGG GAGGAGTTTC TGCCCAATTC TGCAGCTGAA ACTAGAGGAC 
CGTTCTGTGG TGCCCCGAGA TGTGGTCCGG CACATGCGAT CCACCGACAG TCAGTGTGGC 
ACGGTGATCG ACGTCAACAT CGACTGTGCC GTCAAGCTCA TCGGCACCAA CTGCATCATC 
TATCCCGTCA ACAGCAAGGA CCTGCAGCAC ATCTGGCCCT TCATGTATGG GGACTACATT 
GCCTATGACT GCTGGCTGGG GAAGGTCTAC GACTTGAAGA ACCAGATCAT CCTGAAGCTA 
TCCAACGGCG CCAGGTGCTC CATGAACACG GAAGATGGCG CCAAGCTCTA CGACGTCTGC 
CCGCACGTCA GCGACTCGGG TCTCTTCTTC GATGATTCCT ATGGCTTCTA CCCAGGCCAG 
GTGCTCATTG GCCCTGCCAA GATCTTCTCC AGCGTCCAGT GGCTGTCAGG TGTCAAGCCC 
GTGCTCAGCA CCAAGAGCAA GTTCCGAGTG GTGGTGGAAG AGGTGCAGGT TGTAGAGTTG 
AAAGTTACAT GGATTACCAA GAGTTTCTGT CCAGGGGGCA CGGACAGCGT CAGCCCCCCA 
CCCTCTGTCA TCACCCAGGA AAACCTAGGC AGGGTGAAGC GTCTCGGATG CTTTGACCAT 
GCTCAGCGGC AGCTTGGGGA GCGCTGTCTG TATGTCTTCC CAGCCAAGGT GAAGCGCCTG 
TTGAAGAAGC AGGTTGTGCG GATCATGTCA TGCTCCCCAG ACACCCAGTG TTCCCGGGAC 
CATTCCATGG AAGACCCAGA CAAGAAGGGG GAATCCAAAA CCAAGAGCGA AGCGGAGTCT 
GCCAGCCCTG AGGAGACGCC CGATGGCTCT GCCAGTCCAG TGGAGATGCA GGACGAGGGT 
GCAGAGGAGC CCCACGAGGC AGGAGAGCAG CTGCCCCCAT TCCTGCTAAA AGAAGGCAGA 
GATGACAGGC TGCACTCGGC AGAGCAGGAC GCAGATGATG AGGCTGCTGA TGACACGGAC 
GACACCAGTT CGGTGACCTC CTCTGCCAGC TCCACCACTT CCTCCCAGAG CGGCAGCGGC 
ACGAGTCGCA AAAAGAGCAT CCCCTTGTCC ATCAAGAACT TAAAGCGCAA ACACAAGAGG 
AAGAAGAATA AAATCACTCG AGACTTCAAG CCAGGGGACA GGGTGGCAGT GGAGGTGGTG 
ACCACGATGA CCTCAGCCGA CGTGATGTGG CAGGATGGCT CCGTGGAATG CAACATCCGC 
TCCAACGACC TCTTCCCTGT GCACCACCTG GACAACAACG AGTTCTGCCC TGGAGACTTC 
GTGGTAGATA AGCGAGTCCA GAGCTGTCCA GACCCTGCTG TCTACGGTGT GGTACAGTCT 
GGGGACCACA TCGGCCGTAC CTGCATGGTG AAGTGGTTCA AGCTGAGGCC GAGTGGGGAC 
GACGTGGAGC TGATTGGAGA AGAGGAAGAT GTGAGTGTTT ACGACATTGC TGACCACCCT 
GACTTTAGGT TCCGTACAAC TGACATCGTC ATCCGCATCG GCAATACTGA GGATGGGGCT 
CCTCACAAGG AGGATGAGCA GCCATCGGTG GGCCAGGTGG CCCGTGTGGA CGTCAGCAGC 
AAGGTGGAGG TGGTGTGGGC TGACAACTCA AAGACCATCA TCCTGCCCCA GCACTTGTAC 
AACATAGAGT CTGAGATTGA GGAGTCAGAC TACGATTCGG TAGAAGGCAG CACCAGCGGG 
GCATCCTCGG ATGAATGGGA AGATGATAGT GACAGCTGGG AGACGGACAA TGGGCTGGTG 
GAGGACGAGC ACCCCAAGAT AGAGGAGCCC CCCATCCCAC CCCTGGAGCA GCCGGTGGCC 
CCTGAGGACA AGGGAGTGGT GATCAGTGAA GAGGCAGCCA CAGCTGCCGT CCAGGGGGCT 
GTGGCCATGG CTGCCCCCAT GGCCGGGCTG ATGGAGAAGG CTGGCAAGGA CGGGCCACCC 
AAGAGCTTCC GGGAGTTGAA AGAGGCCATC AAGATCCTGG AGAGCCTCAA GAACATGACT 
GTGGAGCAGC TGCTGACGGG CTCGCCCACC TCTCCGACTG TGGAGCCTGA GAAGCCAACT 
CGGGAGAAGA AGTTTCTGGA TGACATCAAG AAGCTACAGG AAAACCTCAA GAAGACCCTG 
GACAATGTGG CCATTGTAGA GGAGGAGAAG ATGGAAGCAG TGCCCGACGT AGAGCGCAAG 
GAGGACAAGC CCGAGGGGCA GTCACCTGTG AAGGCTGAGT GGCCCAGCGA AACCCCGGTG 
CTGTGCCAGC AGTGTGGCGG CAAGCCTGGC GTCACCTTCA CCAGCGCCAA GGGCGAGGTC 
TTCTCCGTAC TGGAGTTTGC ACCCTCAAAT CATTCTTTTA AGAAAATTGA GTTCCAGCCT 
CCAGAAGCCA AGAAGTTCTT CAGCACAGTG CGGAAGGAGA TGGCGCTGCT GGCTACCTCA 
CTGCCTGAGG GCATCATGGT CAAGACTTTT GAAGATAGAA TGGACCTCTT CTCAGCTCTC 
ATCAAGGGCC CCACTCGAAC CCCCTACGAG GATGGCCTCT ACTTGTTTGA CATCCAGCTC 
CCCAACATCT ACCCAGCCGT GCCCCCCCAC TTCTGCTACC TCTCCCAATG CAGTGGCCGC 
CTGAACCCCA ACCTGTATGA CAATGGGAAG GTGTGTGTCA GCCTCCTGGG CACCTGGATT 
GGAAAGGGGA CAGAGAGGTG GACAAGCAAG TCCAGCCTTC TCCAGGTGCT CATCTCCATC 
CAAGGTCTGA TCCTGGTAAA TGAACCATAC TACAACGAAG CCGGCTTCGA CAGTGACCGA 
GGCCTGCAGG AAGGCTATGA AAACAGTCGC TGTTACAATG AGATGGCGCT GATCCGCGTG 
GTGCAGTCCA TGACCCAGCT GGTGCGGCGG CCCCCCGAGG TCTTTGAGCA GGAGATCAGG 
CAACACTTTA GCACTGGTGG CTGGCGGCTG GTGAACCGTA TCGAGTCCTG GCTGGAAACC 
CATGCCCTGC TGGAGAAGGC CCAGGCACTG CCCAACGGGG TGCCCAAGGC CAGCAGCTCG 
CCAGAGCCCC CAGCTGTAGC CGAGCTGTCA GACTCCGGCC AACAAGAACC TGAGGATGGA 
GGGCCAGCCC CAGGAGAGGC CTCCCAGGGC TCAGACTCAG AGGGCGGTGC CCAGGGCCTG 
GCCTCAGCTA GCAGGGACCA CACAGACCAG ACTTCGGAGA CCGCACCAGA CGCATCGGTG 
CCACCCAGTG TGAAACCAAA GAAGCGGAGA AAGAGCTACC GGAGCTTCTT ACCTGAGAAG 
AGTGGCTACC CTGACATCGG CTTCCCCCTC TTCCCACTTT CCAAGGGTTT CATCAAGAGC 
ATCCGGGGTG TCCTGACGCA GTTCCGGGCT GCCCTGCTAG AGGCAGGCAT GCCGGAGTGC 
ACAGAGGACA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306635.1
CDS	94..4008
Translation	MADPAAPTPAAPAPAQAPAPAPEAVPAPAAAPVPAPAPASDSASGPSSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSDSEGEEEGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKAQQQQAAPLNAVSQDSNTSGNSTIMETGRSFCPILQLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVKRLLKKQVVRIMSCSPDTQCSRDHSMEDPDKKGESKTKSEAESASPEETPDGSASPVEMQDEGAEEPHEAGEQLPPFLLKEGRDDRLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGTSRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHIGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAPHKEDEQPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIEEPPIPPLEQPVAPEDKGVVISEEAATAAVQGAVAMAAPMAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIVEEEKMEAVPDVERKEDKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPEGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWLETHALLEKAQALPNGVPKASSSPEPPAVAELSDSGQQEPEDGGPAPGEASQGSDSEGGAQGLASASRDHTDQTSETAPDASVPPSVKPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALLEAGMPECTEDK

Target ORF information:

RefSeq Version	XM_024450867.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024450867.1

ORF Insert Sequence:

ATGGCGGATC CCGCAGCCCC CACGCCCGCA GCTCCCGCTC CAGCCCAGGC CCCGGCTCCA 
GCCCCGGAGG CAGTCCCGGC CCCAGCCGCA GCCCCCGTCC CGGCGCCGGC GCCCGCCTCG 
GACTCGGCCT CCGGGCCGTC CTCGGACTCC GGCCCAGAAG CCGGCTCGCA GCGCCTGCTG 
TTTTCTCACG ACCTGGTGTC GGGCCGTTAC CGTGGCTCCG TGCACTTCGG GCTGGTGCGC 
CTCATCCACG GCGAGGACTC GGACTCGGAG GGCGAGGAGG AGGGCCGCGG GAGCTCGGGG 
TGCTCCGAGG CCGGGGGCGC GGGCCACGAG GAGGGCCGGG CCAGCCCCCT GCGCCGCGGC 
TACGTGCGCG TCCAGTGGTA CCCGGAGGGC GTCAAGCAGC ATGTGAAGGA GACCAAGGCA 
CAACAGCAGC AAGCAGCTCC TCTAAATGCA GTGAGCCAGG ACTCAAATAC CAGCGGAAAC 
AGCACCATCA TGGAGACTGG GAGGAGTTTC TGCCCAATTC TGCAGCTGAA ACTAGAGGAC 
CGTTCTGTGG TGCCCCGAGA TGTGGTCCGG CACATGCGAT CCACCGACAG TCAGTGTGGC 
ACGGTGATCG ACGTCAACAT CGACTGTGCC GTCAAGCTCA TCGGCACCAA CTGCATCATC 
TATCCCGTCA ACAGCAAGGA CCTGCAGCAC ATCTGGCCCT TCATGTATGG GGACTACATT 
GCCTATGACT GCTGGCTGGG GAAGGTCTAC GACTTGAAGA ACCAGATCAT CCTGAAGCTA 
TCCAACGGCG CCAGGTGCTC CATGAACACG GAAGATGGCG CCAAGCTCTA CGACGTCTGC 
CCGCACGTCA GCGACTCGGG TCTCTTCTTC GATGATTCCT ATGGCTTCTA CCCAGGCCAG 
GTGCTCATTG GCCCTGCCAA GATCTTCTCC AGCGTCCAGT GGCTGTCAGG TGTCAAGCCC 
GTGCTCAGCA CCAAGAGCAA GTTCCGAGTG GTGGTGGAAG AGGTGCAGGT TGTAGAGTTG 
AAAGTTACAT GGATTACCAA GAGTTTCTGT CCAGGGGGCA CGGACAGCGT CAGCCCCCCA 
CCCTCTGTCA TCACCCAGGA AAACCTAGGC AGGGTGAAGC GTCTCGGATG CTTTGACCAT 
GCTCAGCGGC AGCTTGGGGA GCGCTGTCTG TATGTCTTCC CAGCCAAGGT GAAGCGCCTG 
TTGAAGAAGC AGGTTGTGCG GATCATGTCA TGCTCCCCAG ACACCCAGTG TTCCCGGGAC 
CATTCCATGG AAGACCCAGA CAAGAAGGGG GAATCCAAAA CCAAGAGCGA AGCGGAGTCT 
GCCAGCCCTG AGGAGACGCC CGATGGCTCT GCCAGTCCAG TGGAGATGCA GGACGAGGGT 
GCAGAGGAGC CCCACGAGGC AGGAGAGCAG CTGCCCCCAT TCCTGCTAAA AGAAGGCAGA 
GATGACAGGC TGCACTCGGC AGAGCAGGAC GCAGATGATG AGGCTGCTGA TGACACGGAC 
GACACCAGTT CGGTGACCTC CTCTGCCAGC TCCACCACTT CCTCCCAGAG CGGCAGCGGC 
ACGAGTCGCA AAAAGAGCAT CCCCTTGTCC ATCAAGAACT TAAAGCGCAA ACACAAGAGG 
AAGAAGAATA AAATCACTCG AGACTTCAAG CCAGGGGACA GGGTGGCAGT GGAGGTGGTG 
ACCACGATGA CCTCAGCCGA CGTGATGTGG CAGGATGGCT CCGTGGAATG CAACATCCGC 
TCCAACGACC TCTTCCCTGT GCACCACCTG GACAACAACG AGTTCTGCCC TGGAGACTTC 
GTGGTAGATA AGCGAGTCCA GAGCTGTCCA GACCCTGCTG TCTACGGTGT GGTACAGTCT 
GGGGACCACA TCGGCCGTAC CTGCATGGTG AAGTGGTTCA AGCTGAGGCC GAGTGGGGAC 
GACGTGGAGC TGATTGGAGA AGAGGAAGAT GTGAGTGTTT ACGACATTGC TGACCACCCT 
GACTTTAGGT TCCGTACAAC TGACATCGTC ATCCGCATCG GCAATACTGA GGATGGGGCT 
CCTCACAAGG AGGATGAGCA GCCATCGGTG GGCCAGGTGG CCCGTGTGGA CGTCAGCAGC 
AAGGTGGAGG TGGTGTGGGC TGACAACTCA AAGACCATCA TCCTGCCCCA GCACTTGTAC 
AACATAGAGT CTGAGATTGA GGAGTCAGAC TACGATTCGG TAGAAGGCAG CACCAGCGGG 
GCATCCTCGG ATGAATGGGA AGATGATAGT GACAGCTGGG AGACGGACAA TGGGCTGGTG 
GAGGACGAGC ACCCCAAGAT AGAGGAGCCC CCCATCCCAC CCCTGGAGCA GCCGGTGGCC 
CCTGAGGACA AGGGAGTGGT GATCAGTGAA GAGGCAGCCA CAGCTGCCGT CCAGGGGGCT 
GTGGCCATGG CTGCCCCCAT GGCCGGGCTG ATGGAGAAGG CTGGCAAGGA CGGGCCACCC 
AAGAGCTTCC GGGAGTTGAA AGAGGCCATC AAGATCCTGG AGAGCCTCAA GAACATGACT 
GTGGAGCAGC TGCTGACGGG CTCGCCCACC TCTCCGACTG TGGAGCCTGA GAAGCCAACT 
CGGGAGAAGA AGTTTCTGGA TGACATCAAG AAGCTACAGG AAAACCTCAA GAAGACCCTG 
GACAATGTGG CCATTGTAGA GGAGGAGAAG ATGGAAGCAG TGCCCGACGT AGAGCGCAAG 
GAGGACAAGC CCGAGGGGCA GTCACCTGTG AAGGCTGAGT GGCCCAGCGA AACCCCGGTG 
CTGTGCCAGC AGTGTGGCGG CAAGCCTGGC GTCACCTTCA CCAGCGCCAA GGGCGAGGTC 
TTCTCCGTAC TGGAGTTTGC ACCCTCAAAT CATTCTTTTA AGAAAATTGA GTTCCAGCCT 
CCAGAAGCCA AGAAGTTCTT CAGCACAGTG CGGAAGGAGA TGGCGCTGCT GGCTACCTCA 
CTGCCTGAGG GCATCATGGT CAAGACTTTT GAAGATAGAA TGGACCTCTT CTCAGCTCTC 
ATCAAGGGCC CCACTCGAAC CCCCTACGAG GATGGCCTCT ACTTGTTTGA CATCCAGCTC 
CCCAACATCT ACCCAGCCGT GCCCCCCCAC TTCTGCTACC TCTCCCAATG CAGTGGCCGC 
CTGAACCCCA ACCTGTATGA CAATGGGAAG GTGTGTGTCA GCCTCCTGGG CACCTGGATT 
GGAAAGGGGA CAGAGAGGTG GACAAGCAAG TCCAGCCTTC TCCAGGTGCT CATCTCCATC 
CAAGGTCTGA TCCTGGTAAA TGAACCATAC TACAACGAAG CCGGCTTCGA CAGTGACCGA 
GGCCTGCAGG AAGGCTATGA AAACAGTCGC TGTTACAATG AGATGGCGCT GATCCGCGTG 
GTGCAGTCCA TGACCCAGCT GGTGCGGCGG CCCCCCGAGG TCTTTGAGCA GGAGATCAGG 
CAACACTTTA GCACTGGTGG CTGGCGGCTG GTGAACCGTA TCGAGTCCTG GCTGGAAACC 
CATGCCCTGC TGGAGAAGGC CCAGGCACTG CCCAACGGGG TGCCCAAGGC CAGCAGCTCG 
CCAGAGCCCC CAGCTGTAGC CGAGCTGTCA GACTCCGGCC AACAAGAACC TGAGGATGGA 
GGGCCAGCCC CAGGAGAGGC CTCCCAGGGC TCAGACTCAG AGGGCGGTGC CCAGGGCCTG 
GCCTCAGCTA GCAGGGACCA CACAGACCAG ACTTCGGAGA CCGCACCAGA CGCATCGGTG 
CCACCCAGTG TGAAACCAAA GAAGCGGAGA AAGAGCTACC GGAGCTTCTT ACCTGAGAAG 
AGTGGCTACC CTGACATCGG CTTCCCCCTC TTCCCACTTT CCAAGGGTTT CATCAAGAGC 
ATCCGGGGTG TCCTGACGCA GTTCCGGGCT GCCCTGCTAG AGGCAGGCAT GCCGGAGTGC 
ACAGAGGACA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2O ( XM_017024934.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016880423.1 Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X2, mRNA.

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CloneID	OHu87027
Clone ID Related Accession (Same CDS sequence)	XM_017024934.1
Accession Version	XM_017024934.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATC CCGCAGCCCC CACGCCCGCA GCTCCCGCTC CAGCCCAGGC CCCGGCTCCA 
GCCCCGGAGG CAGTCCCGGC CCCAGCCGCA GCCCCCGTCC CGGCGCCGGC GCCCGCCTCG 
GACTCGGCCT CCGGGCCGTC CTCGGACTCC GGCCCAGAAG CCGGCTCGCA GCGCCTGCTG 
TTTTCTCACG ACCTGGTGTC GGGCCGTTAC CGTGGCTCCG TGCACTTCGG GCTGGTGCGC 
CTCATCCACG GCGAGGACTC GGACTCGGAG GGCGAGGAGG AGGGCCGCGG GAGCTCGGGG 
TGCTCCGAGG CCGGGGGCGC GGGCCACGAG GAGGGCCGGG CCAGCCCCCT GCGCCGCGGC 
TACGTGCGCG TCCAGTGGTA CCCGGAGGGC GTCAAGCAGC ATGTGAAGGA GACCAAGCTG 
AAACTAGAGG ACCGTTCTGT GGTGCCCCGA GATGTGGTCC GGCACATGCG ATCCACCGAC 
AGTCAGTGTG GCACGGTGAT CGACGTCAAC ATCGACTGTG CCGTCAAGCT CATCGGCACC 
AACTGCATCA TCTATCCCGT CAACAGCAAG GACCTGCAGC ACATCTGGCC CTTCATGTAT 
GGGGACTACA TTGCCTATGA CTGCTGGCTG GGGAAGGTCT ACGACTTGAA GAACCAGATC 
ATCCTGAAGC TATCCAACGG CGCCAGGTGC TCCATGAACA CGGAAGATGG CGCCAAGCTC 
TACGACGTCT GCCCGCACGT CAGCGACTCG GGTCTCTTCT TCGATGATTC CTATGGCTTC 
TACCCAGGCC AGGTGCTCAT TGGCCCTGCC AAGATCTTCT CCAGCGTCCA GTGGCTGTCA 
GGTGTCAAGC CCGTGCTCAG CACCAAGAGC AAGTTCCGAG TGGTGGTGGA AGAGGTGCAG 
GTTGTAGAGT TGAAAGTTAC ATGGATTACC AAGAGTTTCT GTCCAGGGGG CACGGACAGC 
GTCAGCCCCC CACCCTCTGT CATCACCCAG GAAAACCTAG GCAGGGTGAA GCGTCTCGGA 
TGCTTTGACC ATGCTCAGCG GCAGCTTGGG GAGCGCTGTC TGTATGTCTT CCCAGCCAAG 
GTGAAGCGCC TGTTGAAGAA GCAGGTTGTG CGGATCATGT CATGCTCCCC AGACACCCAG 
TGTTCCCGGG ACCATTCCAT GGAAGACCCA GACAAGAAGG GGGAATCCAA AACCAAGAGC 
GAAGCGGAGT CTGCCAGCCC TGAGGAGACG CCCGATGGCT CTGCCAGTCC AGTGGAGATG 
CAGGACGAGG GTGCAGAGGA GCCCCACGAG GCAGGAGAGC AGCTGCCCCC ATTCCTGCTA 
AAAGAAGGCA GAGATGACAG GCTGCACTCG GCAGAGCAGG ACGCAGATGA TGAGGCTGCT 
GATGACACGG ACGACACCAG TTCGGTGACC TCCTCTGCCA GCTCCACCAC TTCCTCCCAG 
AGCGGCAGCG GCACGAGTCG CAAAAAGAGC ATCCCCTTGT CCATCAAGAA CTTAAAGCGC 
AAACACAAGA GGAAGAAGAA TAAAATCACT CGAGACTTCA AGCCAGGGGA CAGGGTGGCA 
GTGGAGGTGG TGACCACGAT GACCTCAGCC GACGTGATGT GGCAGGATGG CTCCGTGGAA 
TGCAACATCC GCTCCAACGA CCTCTTCCCT GTGCACCACC TGGACAACAA CGAGTTCTGC 
CCTGGAGACT TCGTGGTAGA TAAGCGAGTC CAGAGCTGTC CAGACCCTGC TGTCTACGGT 
GTGGTACAGT CTGGGGACCA CATCGGCCGT ACCTGCATGG TGAAGTGGTT CAAGCTGAGG 
CCGAGTGGGG ACGACGTGGA GCTGATTGGA GAAGAGGAAG ATGTGAGTGT TTACGACATT 
GCTGACCACC CTGACTTTAG GTTCCGTACA ACTGACATCG TCATCCGCAT CGGCAATACT 
GAGGATGGGG CTCCTCACAA GGAGGATGAG CAGCCATCGG TGGGCCAGGT GGCCCGTGTG 
GACGTCAGCA GCAAGGTGGA GGTGGTGTGG GCTGACAACT CAAAGACCAT CATCCTGCCC 
CAGCACTTGT ACAACATAGA GTCTGAGATT GAGGAGTCAG ACTACGATTC GGTAGAAGGC 
AGCACCAGCG GGGCATCCTC GGATGAATGG GAAGATGATA GTGACAGCTG GGAGACGGAC 
AATGGGCTGG TGGAGGACGA GCACCCCAAG ATAGAGGAGC CCCCCATCCC ACCCCTGGAG 
CAGCCGGTGG CCCCTGAGGA CAAGGGAGTG GTGATCAGTG AAGAGGCAGC CACAGCTGCC 
GTCCAGGGGG CTGTGGCCAT GGCTGCCCCC ATGGCCGGGC TGATGGAGAA GGCTGGCAAG 
GACGGGCCAC CCAAGAGCTT CCGGGAGTTG AAAGAGGCCA TCAAGATCCT GGAGAGCCTC 
AAGAACATGA CTGTGGAGCA GCTGCTGACG GGCTCGCCCA CCTCTCCGAC TGTGGAGCCT 
GAGAAGCCAA CTCGGGAGAA GAAGTTTCTG GATGACATCA AGAAGCTACA GGAAAACCTC 
AAGAAGACCC TGGACAATGT GGCCATTGTA GAGGAGGAGA AGATGGAAGC AGTGCCCGAC 
GTAGAGCGCA AGGAGGACAA GCCCGAGGGG CAGTCACCTG TGAAGGCTGA GTGGCCCAGC 
GAAACCCCGG TGCTGTGCCA GCAGTGTGGC GGCAAGCCTG GCGTCACCTT CACCAGCGCC 
AAGGGCGAGG TCTTCTCCGT ACTGGAGTTT GCACCCTCAA ATCATTCTTT TAAGAAAATT 
GAGTTCCAGC CTCCAGAAGC CAAGAAGTTC TTCAGCACAG TGCGGAAGGA GATGGCGCTG 
CTGGCTACCT CACTGCCTGA GGGCATCATG GTCAAGACTT TTGAAGATAG AATGGACCTC 
TTCTCAGCTC TCATCAAGGG CCCCACTCGA ACCCCCTACG AGGATGGCCT CTACTTGTTT 
GACATCCAGC TCCCCAACAT CTACCCAGCC GTGCCCCCCC ACTTCTGCTA CCTCTCCCAA 
TGCAGTGGCC GCCTGAACCC CAACCTGTAT GACAATGGGA AGGTGTGTGT CAGCCTCCTG 
GGCACCTGGA TTGGAAAGGG GACAGAGAGG TGGACAAGCA AGTCCAGCCT TCTCCAGGTG 
CTCATCTCCA TCCAAGGTCT GATCCTGGTA AATGAACCAT ACTACAACGA AGCCGGCTTC 
GACAGTGACC GAGGCCTGCA GGAAGGCTAT GAAAACAGTC GCTGTTACAA TGAGATGGCG 
CTGATCCGCG TGGTGCAGTC CATGACCCAG CTGGTGCGGC GGCCCCCCGA GGTCTTTGAG 
CAGGAGATCA GGCAACACTT TAGCACTGGT GGCTGGCGGC TGGTGAACCG TATCGAGTCC 
TGGCTGGAAA CCCATGCCCT GCTGGAGAAG GCCCAGGCAC TGCCCAACGG GGTGCCCAAG 
GCCAGCAGCT CGCCAGAGCC CCCAGCTGTA GCCGAGCTGT CAGACTCCGG CCAACAAGAA 
CCTGAGGATG GAGGGCCAGC CCCAGGAGAG GCCTCCCAGG GCTCAGACTC AGAGGGCGGT 
GCCCAGGGCC TGGCCTCAGC TAGCAGGGAC CACACAGACC AGACTTCGGA GACCGCACCA 
GACGCATCGG TGCCACCCAG TGTGAAACCA AAGAAGCGGA GAAAGAGCTA CCGGAGCTTC 
TTACCTGAGA AGAGTGGCTA CCCTGACATC GGCTTCCCCC TCTTCCCACT TTCCAAGGGT 
TTCATCAAGA GCATCCGGGG TGTCCTGACG CAGTTCCGGG CTGCCCTGCT AGAGGCAGGC 
ATGCCGGAGT GCACAGAGGA CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016880423.1
CDS	50..3856
Translation	MADPAAPTPAAPAPAQAPAPAPEAVPAPAAAPVPAPAPASDSASGPSSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSDSEGEEEGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVKRLLKKQVVRIMSCSPDTQCSRDHSMEDPDKKGESKTKSEAESASPEETPDGSASPVEMQDEGAEEPHEAGEQLPPFLLKEGRDDRLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGTSRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHIGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAPHKEDEQPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIEEPPIPPLEQPVAPEDKGVVISEEAATAAVQGAVAMAAPMAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIVEEEKMEAVPDVERKEDKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPEGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWLETHALLEKAQALPNGVPKASSSPEPPAVAELSDSGQQEPEDGGPAPGEASQGSDSEGGAQGLASASRDHTDQTSETAPDASVPPSVKPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALLEAGMPECTEDK

Target ORF information:

RefSeq Version	XM_017024934.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024934.1

ORF Insert Sequence:

ATGGCGGATC CCGCAGCCCC CACGCCCGCA GCTCCCGCTC CAGCCCAGGC CCCGGCTCCA 
GCCCCGGAGG CAGTCCCGGC CCCAGCCGCA GCCCCCGTCC CGGCGCCGGC GCCCGCCTCG 
GACTCGGCCT CCGGGCCGTC CTCGGACTCC GGCCCAGAAG CCGGCTCGCA GCGCCTGCTG 
TTTTCTCACG ACCTGGTGTC GGGCCGTTAC CGTGGCTCCG TGCACTTCGG GCTGGTGCGC 
CTCATCCACG GCGAGGACTC GGACTCGGAG GGCGAGGAGG AGGGCCGCGG GAGCTCGGGG 
TGCTCCGAGG CCGGGGGCGC GGGCCACGAG GAGGGCCGGG CCAGCCCCCT GCGCCGCGGC 
TACGTGCGCG TCCAGTGGTA CCCGGAGGGC GTCAAGCAGC ATGTGAAGGA GACCAAGCTG 
AAACTAGAGG ACCGTTCTGT GGTGCCCCGA GATGTGGTCC GGCACATGCG ATCCACCGAC 
AGTCAGTGTG GCACGGTGAT CGACGTCAAC ATCGACTGTG CCGTCAAGCT CATCGGCACC 
AACTGCATCA TCTATCCCGT CAACAGCAAG GACCTGCAGC ACATCTGGCC CTTCATGTAT 
GGGGACTACA TTGCCTATGA CTGCTGGCTG GGGAAGGTCT ACGACTTGAA GAACCAGATC 
ATCCTGAAGC TATCCAACGG CGCCAGGTGC TCCATGAACA CGGAAGATGG CGCCAAGCTC 
TACGACGTCT GCCCGCACGT CAGCGACTCG GGTCTCTTCT TCGATGATTC CTATGGCTTC 
TACCCAGGCC AGGTGCTCAT TGGCCCTGCC AAGATCTTCT CCAGCGTCCA GTGGCTGTCA 
GGTGTCAAGC CCGTGCTCAG CACCAAGAGC AAGTTCCGAG TGGTGGTGGA AGAGGTGCAG 
GTTGTAGAGT TGAAAGTTAC ATGGATTACC AAGAGTTTCT GTCCAGGGGG CACGGACAGC 
GTCAGCCCCC CACCCTCTGT CATCACCCAG GAAAACCTAG GCAGGGTGAA GCGTCTCGGA 
TGCTTTGACC ATGCTCAGCG GCAGCTTGGG GAGCGCTGTC TGTATGTCTT CCCAGCCAAG 
GTGAAGCGCC TGTTGAAGAA GCAGGTTGTG CGGATCATGT CATGCTCCCC AGACACCCAG 
TGTTCCCGGG ACCATTCCAT GGAAGACCCA GACAAGAAGG GGGAATCCAA AACCAAGAGC 
GAAGCGGAGT CTGCCAGCCC TGAGGAGACG CCCGATGGCT CTGCCAGTCC AGTGGAGATG 
CAGGACGAGG GTGCAGAGGA GCCCCACGAG GCAGGAGAGC AGCTGCCCCC ATTCCTGCTA 
AAAGAAGGCA GAGATGACAG GCTGCACTCG GCAGAGCAGG ACGCAGATGA TGAGGCTGCT 
GATGACACGG ACGACACCAG TTCGGTGACC TCCTCTGCCA GCTCCACCAC TTCCTCCCAG 
AGCGGCAGCG GCACGAGTCG CAAAAAGAGC ATCCCCTTGT CCATCAAGAA CTTAAAGCGC 
AAACACAAGA GGAAGAAGAA TAAAATCACT CGAGACTTCA AGCCAGGGGA CAGGGTGGCA 
GTGGAGGTGG TGACCACGAT GACCTCAGCC GACGTGATGT GGCAGGATGG CTCCGTGGAA 
TGCAACATCC GCTCCAACGA CCTCTTCCCT GTGCACCACC TGGACAACAA CGAGTTCTGC 
CCTGGAGACT TCGTGGTAGA TAAGCGAGTC CAGAGCTGTC CAGACCCTGC TGTCTACGGT 
GTGGTACAGT CTGGGGACCA CATCGGCCGT ACCTGCATGG TGAAGTGGTT CAAGCTGAGG 
CCGAGTGGGG ACGACGTGGA GCTGATTGGA GAAGAGGAAG ATGTGAGTGT TTACGACATT 
GCTGACCACC CTGACTTTAG GTTCCGTACA ACTGACATCG TCATCCGCAT CGGCAATACT 
GAGGATGGGG CTCCTCACAA GGAGGATGAG CAGCCATCGG TGGGCCAGGT GGCCCGTGTG 
GACGTCAGCA GCAAGGTGGA GGTGGTGTGG GCTGACAACT CAAAGACCAT CATCCTGCCC 
CAGCACTTGT ACAACATAGA GTCTGAGATT GAGGAGTCAG ACTACGATTC GGTAGAAGGC 
AGCACCAGCG GGGCATCCTC GGATGAATGG GAAGATGATA GTGACAGCTG GGAGACGGAC 
AATGGGCTGG TGGAGGACGA GCACCCCAAG ATAGAGGAGC CCCCCATCCC ACCCCTGGAG 
CAGCCGGTGG CCCCTGAGGA CAAGGGAGTG GTGATCAGTG AAGAGGCAGC CACAGCTGCC 
GTCCAGGGGG CTGTGGCCAT GGCTGCCCCC ATGGCCGGGC TGATGGAGAA GGCTGGCAAG 
GACGGGCCAC CCAAGAGCTT CCGGGAGTTG AAAGAGGCCA TCAAGATCCT GGAGAGCCTC 
AAGAACATGA CTGTGGAGCA GCTGCTGACG GGCTCGCCCA CCTCTCCGAC TGTGGAGCCT 
GAGAAGCCAA CTCGGGAGAA GAAGTTTCTG GATGACATCA AGAAGCTACA GGAAAACCTC 
AAGAAGACCC TGGACAATGT GGCCATTGTA GAGGAGGAGA AGATGGAAGC AGTGCCCGAC 
GTAGAGCGCA AGGAGGACAA GCCCGAGGGG CAGTCACCTG TGAAGGCTGA GTGGCCCAGC 
GAAACCCCGG TGCTGTGCCA GCAGTGTGGC GGCAAGCCTG GCGTCACCTT CACCAGCGCC 
AAGGGCGAGG TCTTCTCCGT ACTGGAGTTT GCACCCTCAA ATCATTCTTT TAAGAAAATT 
GAGTTCCAGC CTCCAGAAGC CAAGAAGTTC TTCAGCACAG TGCGGAAGGA GATGGCGCTG 
CTGGCTACCT CACTGCCTGA GGGCATCATG GTCAAGACTT TTGAAGATAG AATGGACCTC 
TTCTCAGCTC TCATCAAGGG CCCCACTCGA ACCCCCTACG AGGATGGCCT CTACTTGTTT 
GACATCCAGC TCCCCAACAT CTACCCAGCC GTGCCCCCCC ACTTCTGCTA CCTCTCCCAA 
TGCAGTGGCC GCCTGAACCC CAACCTGTAT GACAATGGGA AGGTGTGTGT CAGCCTCCTG 
GGCACCTGGA TTGGAAAGGG GACAGAGAGG TGGACAAGCA AGTCCAGCCT TCTCCAGGTG 
CTCATCTCCA TCCAAGGTCT GATCCTGGTA AATGAACCAT ACTACAACGA AGCCGGCTTC 
GACAGTGACC GAGGCCTGCA GGAAGGCTAT GAAAACAGTC GCTGTTACAA TGAGATGGCG 
CTGATCCGCG TGGTGCAGTC CATGACCCAG CTGGTGCGGC GGCCCCCCGA GGTCTTTGAG 
CAGGAGATCA GGCAACACTT TAGCACTGGT GGCTGGCGGC TGGTGAACCG TATCGAGTCC 
TGGCTGGAAA CCCATGCCCT GCTGGAGAAG GCCCAGGCAC TGCCCAACGG GGTGCCCAAG 
GCCAGCAGCT CGCCAGAGCC CCCAGCTGTA GCCGAGCTGT CAGACTCCGG CCAACAAGAA 
CCTGAGGATG GAGGGCCAGC CCCAGGAGAG GCCTCCCAGG GCTCAGACTC AGAGGGCGGT 
GCCCAGGGCC TGGCCTCAGC TAGCAGGGAC CACACAGACC AGACTTCGGA GACCGCACCA 
GACGCATCGG TGCCACCCAG TGTGAAACCA AAGAAGCGGA GAAAGAGCTA CCGGAGCTTC 
TTACCTGAGA AGAGTGGCTA CCCTGACATC GGCTTCCCCC TCTTCCCACT TTCCAAGGGT 
TTCATCAAGA GCATCCGGGG TGTCCTGACG CAGTTCCGGG CTGCCCTGCT AGAGGCAGGC 
ATGCCGGAGT GCACAGAGGA CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2O ( XM_017024935.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016880424.1 Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X5, mRNA.

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CloneID	OHu87028
Clone ID Related Accession (Same CDS sequence)	XM_017024935.1
Accession Version	XM_017024935.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATC CCGCAGCCCC CACGCCCGCA GCTCCCGCTC CAGCCCAGGC CCCGGCTCCA 
GCCCCGGAGG CAGTCCCGGC CCCAGCCGCA GCCCCCGTCC CGGCGCCGGC GCCCGCCTCG 
GACTCGGCCT CCGGGCCGTC CTCGGACTCC GGCCCAGAAG CCGGCTCGCA GCGCCTGCTG 
TTTTCTCACG ACCTGGTGTC GGGCCGTTAC CGTGGCTCCG TGCACTTCGG GCTGGTGCGC 
CTCATCCACG GCGAGGACTC GGACTCGGAG GGCGAGGAGG AGGGCCGCGG GAGCTCGGGG 
TGCTCCGAGG CCGGGGGCGC GGGCCACGAG GAGGGCCGGG CCAGCCCCCT GCGCCGCGGC 
TACGTGCGCG TCCAGTGGTA CCCGGAGGGC GTCAAGCAGC ATGTGAAGGA GACCAAGCTG 
AAACTAGAGG ACCGTTCTGT GGTGCCCCGA GATGTGGTCC GGCACATGCG ATCCACCGAC 
AGTCAGTGTG GCACGGTGAT CGACGTCAAC ATCGACTGTG CCGTCAAGCT CATCGGCACC 
AACTGCATCA TCTATCCCGT CAACAGCAAG GACCTGCAGC ACATCTGGCC CTTCATGTAT 
GGGGACTACA TTGCCTATGA CTGCTGGCTG GGGAAGGTCT ACGACTTGAA GAACCAGATC 
ATCCTGAAGC TATCCAACGG CGCCAGGTGC TCCATGAACA CGGAAGATGG CGCCAAGCTC 
TACGACGTCT GCCCGCACGT CAGCGACTCG GGTCTCTTCT TCGATGATTC CTATGGCTTC 
TACCCAGGCC AGGTGCTCAT TGGCCCTGCC AAGATCTTCT CCAGCGTCCA GTGGCTGTCA 
GGTGTCAAGC CCGTGCTCAG CACCAAGAGC AAGTTCCGAG TGGTGGTGGA AGAGGTGCAG 
GTTGTAGAGT TGAAAGTTAC ATGGATTACC AAGAGTTTCT GTCCAGGGGG CACGGACAGC 
GTCAGCCCCC CACCCTCTGT CATCACCCAG GAAAACCTAG GCAGGGTGAA GCGTCTCGGA 
TGCTTTGACC ATGCTCAGCG GCAGCTTGGG GAGCGCTGTC TGTATGTCTT CCCAGCCAAG 
GTGAAGCGCC TGTTGAAGAA GCAGGTTGTG CGGATCATGT CATGCTCCCC AGACACCCAG 
TGTTCCCGGG ACCATTCCAT GGAAGACCCA GACAAGAAGG GGGAATCCAA AACCAAGAGC 
GAAGCGGAGT CTGCCAGCCC TGAGGAGACG CCCGATGGCT CTGCCAGTCC AGTGGAGATG 
CAGGACGAGG GTGCAGAGGA GCCCCACGAG GCAGGAGAGC AGCTGCCCCC ATTCCTGCTA 
AAAGAAGGCA GAGATGACAG GCTGCACTCG GCAGAGCAGG ACGCAGATGA TGAGGCTGCT 
GATGACACGG ACGACACCAG TTCGGTGACC TCCTCTGCCA GCTCCACCAC TTCCTCCCAG 
AGCGGCAGCG GCACGAGTCG CAAAAAGAGC ATCCCCTTGT CCATCAAGAA CTTAAAGCGC 
AAACACAAGA GGAAGAAGAA TAAAATCACT CGAGACTTCA AGCCAGGGGA CAGGGTGGCA 
GTGGAGGTGG TGACCACGAT GACCTCAGCC GACGTGATGT GGCAGGATGG CTCCGTGGAA 
TGCAACATCC GCTCCAACGA CCTCTTCCCT GTGCACCACC TGGACAACAA CGAGTTCTGC 
CCTGGAGACT TCGTGGTAGA TAAGCGAGTC CAGAGCTGTC CAGACCCTGC TGTCTACGGT 
GTGGTACAGT CTGGGGACCA CATCGGCCGT ACCTGCATGG TGAAGTGGTT CAAGCTGAGG 
CCGAGTGGGG ACGACGTGGA GCTGATTGGA GAAGAGGAAG ATGTGAGTGT TTACGACATT 
GCTGACCACC CTGACTTTAG GTTCCGTACA ACTGACATCG TCATCCGCAT CGGCAATACT 
GAGGATGGGG CTCCTCACAA GGAGGATGAG CCATCGGTGG GCCAGGTGGC CCGTGTGGAC 
GTCAGCAGCA AGGTGGAGGT GGTGTGGGCT GACAACTCAA AGACCATCAT CCTGCCCCAG 
CACTTGTACA ACATAGAGTC TGAGATTGAG GAGTCAGACT ACGATTCGGT AGAAGGCAGC 
ACCAGCGGGG CATCCTCGGA TGAATGGGAA GATGATAGTG ACAGCTGGGA GACGGACAAT 
GGGCTGGTGG AGGACGAGCA CCCCAAGATA GAGGAGCCCC CCATCCCACC CCTGGAGCAG 
CCGGTGGCCC CTGAGGACAA GGGAGTGGTG ATCAGTGAAG AGGCAGCCAC AGCTGCCGTC 
CAGGGGGCTG TGGCCATGGC TGCCCCCATG GCCGGGCTGA TGGAGAAGGC TGGCAAGGAC 
GGGCCACCCA AGAGCTTCCG GGAGTTGAAA GAGGCCATCA AGATCCTGGA GAGCCTCAAG 
AACATGACTG TGGAGCAGCT GCTGACGGGC TCGCCCACCT CTCCGACTGT GGAGCCTGAG 
AAGCCAACTC GGGAGAAGAA GTTTCTGGAT GACATCAAGA AGCTACAGGA AAACCTCAAG 
AAGACCCTGG ACAATGTGGC CATTGTAGAG GAGGAGAAGA TGGAAGCAGT GCCCGACGTA 
GAGCGCAAGG AGGACAAGCC CGAGGGGCAG TCACCTGTGA AGGCTGAGTG GCCCAGCGAA 
ACCCCGGTGC TGTGCCAGCA GTGTGGCGGC AAGCCTGGCG TCACCTTCAC CAGCGCCAAG 
GGCGAGGTCT TCTCCGTACT GGAGTTTGCA CCCTCAAATC ATTCTTTTAA GAAAATTGAG 
TTCCAGCCTC CAGAAGCCAA GAAGTTCTTC AGCACAGTGC GGAAGGAGAT GGCGCTGCTG 
GCTACCTCAC TGCCTGAGGG CATCATGGTC AAGACTTTTG AAGATAGAAT GGACCTCTTC 
TCAGCTCTCA TCAAGGGCCC CACTCGAACC CCCTACGAGG ATGGCCTCTA CTTGTTTGAC 
ATCCAGCTCC CCAACATCTA CCCAGCCGTG CCCCCCCACT TCTGCTACCT CTCCCAATGC 
AGTGGCCGCC TGAACCCCAA CCTGTATGAC AATGGGAAGG TGTGTGTCAG CCTCCTGGGC 
ACCTGGATTG GAAAGGGGAC AGAGAGGTGG ACAAGCAAGT CCAGCCTTCT CCAGGTGCTC 
ATCTCCATCC AAGGTCTGAT CCTGGTAAAT GAACCATACT ACAACGAAGC CGGCTTCGAC 
AGTGACCGAG GCCTGCAGGA AGGCTATGAA AACAGTCGCT GTTACAATGA GATGGCGCTG 
ATCCGCGTGG TGCAGTCCAT GACCCAGCTG GTGCGGCGGC CCCCCGAGGT CTTTGAGCAG 
GAGATCAGGC AACACTTTAG CACTGGTGGC TGGCGGCTGG TGAACCGTAT CGAGTCCTGG 
CTGGAAACCC ATGCCCTGCT GGAGAAGGCC CAGGCACTGC CCAACGGGGT GCCCAAGGCC 
AGCAGCTCGC CAGAGCCCCC AGCTGTAGCC GAGCTGTCAG ACTCCGGCCA ACAAGAACCT 
GAGGATGGAG GGCCAGCCCC AGGAGAGGCC TCCCAGGGCT CAGACTCAGA GGGCGGTGCC 
CAGGGCCTGG CCTCAGCTAG CAGGGACCAC ACAGACCAGA CTTCGGAGAC CGCACCAGAC 
GCATCGGTGC CACCCAGTGT GAAACCAAAG AAGCGGAGAA AGAGCTACCG GAGCTTCTTA 
CCTGAGAAGA GTGGCTACCC TGACATCGGC TTCCCCCTCT TCCCACTTTC CAAGGGTTTC 
ATCAAGAGCA TCCGGGGTGT CCTGACGCAG TTCCGGGCTG CCCTGCTAGA GGCAGGCATG 
CCGGAGTGCA CAGAGGACAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016880424.1
CDS	50..3853
Translation	MADPAAPTPAAPAPAQAPAPAPEAVPAPAAAPVPAPAPASDSASGPSSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSDSEGEEEGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVKRLLKKQVVRIMSCSPDTQCSRDHSMEDPDKKGESKTKSEAESASPEETPDGSASPVEMQDEGAEEPHEAGEQLPPFLLKEGRDDRLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGTSRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHIGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAPHKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIEEPPIPPLEQPVAPEDKGVVISEEAATAAVQGAVAMAAPMAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIVEEEKMEAVPDVERKEDKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPEGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWLETHALLEKAQALPNGVPKASSSPEPPAVAELSDSGQQEPEDGGPAPGEASQGSDSEGGAQGLASASRDHTDQTSETAPDASVPPSVKPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALLEAGMPECTEDK

Target ORF information:

RefSeq Version	XM_017024935.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024935.1

ORF Insert Sequence:

ATGGCGGATC CCGCAGCCCC CACGCCCGCA GCTCCCGCTC CAGCCCAGGC CCCGGCTCCA 
GCCCCGGAGG CAGTCCCGGC CCCAGCCGCA GCCCCCGTCC CGGCGCCGGC GCCCGCCTCG 
GACTCGGCCT CCGGGCCGTC CTCGGACTCC GGCCCAGAAG CCGGCTCGCA GCGCCTGCTG 
TTTTCTCACG ACCTGGTGTC GGGCCGTTAC CGTGGCTCCG TGCACTTCGG GCTGGTGCGC 
CTCATCCACG GCGAGGACTC GGACTCGGAG GGCGAGGAGG AGGGCCGCGG GAGCTCGGGG 
TGCTCCGAGG CCGGGGGCGC GGGCCACGAG GAGGGCCGGG CCAGCCCCCT GCGCCGCGGC 
TACGTGCGCG TCCAGTGGTA CCCGGAGGGC GTCAAGCAGC ATGTGAAGGA GACCAAGCTG 
AAACTAGAGG ACCGTTCTGT GGTGCCCCGA GATGTGGTCC GGCACATGCG ATCCACCGAC 
AGTCAGTGTG GCACGGTGAT CGACGTCAAC ATCGACTGTG CCGTCAAGCT CATCGGCACC 
AACTGCATCA TCTATCCCGT CAACAGCAAG GACCTGCAGC ACATCTGGCC CTTCATGTAT 
GGGGACTACA TTGCCTATGA CTGCTGGCTG GGGAAGGTCT ACGACTTGAA GAACCAGATC 
ATCCTGAAGC TATCCAACGG CGCCAGGTGC TCCATGAACA CGGAAGATGG CGCCAAGCTC 
TACGACGTCT GCCCGCACGT CAGCGACTCG GGTCTCTTCT TCGATGATTC CTATGGCTTC 
TACCCAGGCC AGGTGCTCAT TGGCCCTGCC AAGATCTTCT CCAGCGTCCA GTGGCTGTCA 
GGTGTCAAGC CCGTGCTCAG CACCAAGAGC AAGTTCCGAG TGGTGGTGGA AGAGGTGCAG 
GTTGTAGAGT TGAAAGTTAC ATGGATTACC AAGAGTTTCT GTCCAGGGGG CACGGACAGC 
GTCAGCCCCC CACCCTCTGT CATCACCCAG GAAAACCTAG GCAGGGTGAA GCGTCTCGGA 
TGCTTTGACC ATGCTCAGCG GCAGCTTGGG GAGCGCTGTC TGTATGTCTT CCCAGCCAAG 
GTGAAGCGCC TGTTGAAGAA GCAGGTTGTG CGGATCATGT CATGCTCCCC AGACACCCAG 
TGTTCCCGGG ACCATTCCAT GGAAGACCCA GACAAGAAGG GGGAATCCAA AACCAAGAGC 
GAAGCGGAGT CTGCCAGCCC TGAGGAGACG CCCGATGGCT CTGCCAGTCC AGTGGAGATG 
CAGGACGAGG GTGCAGAGGA GCCCCACGAG GCAGGAGAGC AGCTGCCCCC ATTCCTGCTA 
AAAGAAGGCA GAGATGACAG GCTGCACTCG GCAGAGCAGG ACGCAGATGA TGAGGCTGCT 
GATGACACGG ACGACACCAG TTCGGTGACC TCCTCTGCCA GCTCCACCAC TTCCTCCCAG 
AGCGGCAGCG GCACGAGTCG CAAAAAGAGC ATCCCCTTGT CCATCAAGAA CTTAAAGCGC 
AAACACAAGA GGAAGAAGAA TAAAATCACT CGAGACTTCA AGCCAGGGGA CAGGGTGGCA 
GTGGAGGTGG TGACCACGAT GACCTCAGCC GACGTGATGT GGCAGGATGG CTCCGTGGAA 
TGCAACATCC GCTCCAACGA CCTCTTCCCT GTGCACCACC TGGACAACAA CGAGTTCTGC 
CCTGGAGACT TCGTGGTAGA TAAGCGAGTC CAGAGCTGTC CAGACCCTGC TGTCTACGGT 
GTGGTACAGT CTGGGGACCA CATCGGCCGT ACCTGCATGG TGAAGTGGTT CAAGCTGAGG 
CCGAGTGGGG ACGACGTGGA GCTGATTGGA GAAGAGGAAG ATGTGAGTGT TTACGACATT 
GCTGACCACC CTGACTTTAG GTTCCGTACA ACTGACATCG TCATCCGCAT CGGCAATACT 
GAGGATGGGG CTCCTCACAA GGAGGATGAG CCATCGGTGG GCCAGGTGGC CCGTGTGGAC 
GTCAGCAGCA AGGTGGAGGT GGTGTGGGCT GACAACTCAA AGACCATCAT CCTGCCCCAG 
CACTTGTACA ACATAGAGTC TGAGATTGAG GAGTCAGACT ACGATTCGGT AGAAGGCAGC 
ACCAGCGGGG CATCCTCGGA TGAATGGGAA GATGATAGTG ACAGCTGGGA GACGGACAAT 
GGGCTGGTGG AGGACGAGCA CCCCAAGATA GAGGAGCCCC CCATCCCACC CCTGGAGCAG 
CCGGTGGCCC CTGAGGACAA GGGAGTGGTG ATCAGTGAAG AGGCAGCCAC AGCTGCCGTC 
CAGGGGGCTG TGGCCATGGC TGCCCCCATG GCCGGGCTGA TGGAGAAGGC TGGCAAGGAC 
GGGCCACCCA AGAGCTTCCG GGAGTTGAAA GAGGCCATCA AGATCCTGGA GAGCCTCAAG 
AACATGACTG TGGAGCAGCT GCTGACGGGC TCGCCCACCT CTCCGACTGT GGAGCCTGAG 
AAGCCAACTC GGGAGAAGAA GTTTCTGGAT GACATCAAGA AGCTACAGGA AAACCTCAAG 
AAGACCCTGG ACAATGTGGC CATTGTAGAG GAGGAGAAGA TGGAAGCAGT GCCCGACGTA 
GAGCGCAAGG AGGACAAGCC CGAGGGGCAG TCACCTGTGA AGGCTGAGTG GCCCAGCGAA 
ACCCCGGTGC TGTGCCAGCA GTGTGGCGGC AAGCCTGGCG TCACCTTCAC CAGCGCCAAG 
GGCGAGGTCT TCTCCGTACT GGAGTTTGCA CCCTCAAATC ATTCTTTTAA GAAAATTGAG 
TTCCAGCCTC CAGAAGCCAA GAAGTTCTTC AGCACAGTGC GGAAGGAGAT GGCGCTGCTG 
GCTACCTCAC TGCCTGAGGG CATCATGGTC AAGACTTTTG AAGATAGAAT GGACCTCTTC 
TCAGCTCTCA TCAAGGGCCC CACTCGAACC CCCTACGAGG ATGGCCTCTA CTTGTTTGAC 
ATCCAGCTCC CCAACATCTA CCCAGCCGTG CCCCCCCACT TCTGCTACCT CTCCCAATGC 
AGTGGCCGCC TGAACCCCAA CCTGTATGAC AATGGGAAGG TGTGTGTCAG CCTCCTGGGC 
ACCTGGATTG GAAAGGGGAC AGAGAGGTGG ACAAGCAAGT CCAGCCTTCT CCAGGTGCTC 
ATCTCCATCC AAGGTCTGAT CCTGGTAAAT GAACCATACT ACAACGAAGC CGGCTTCGAC 
AGTGACCGAG GCCTGCAGGA AGGCTATGAA AACAGTCGCT GTTACAATGA GATGGCGCTG 
ATCCGCGTGG TGCAGTCCAT GACCCAGCTG GTGCGGCGGC CCCCCGAGGT CTTTGAGCAG 
GAGATCAGGC AACACTTTAG CACTGGTGGC TGGCGGCTGG TGAACCGTAT CGAGTCCTGG 
CTGGAAACCC ATGCCCTGCT GGAGAAGGCC CAGGCACTGC CCAACGGGGT GCCCAAGGCC 
AGCAGCTCGC CAGAGCCCCC AGCTGTAGCC GAGCTGTCAG ACTCCGGCCA ACAAGAACCT 
GAGGATGGAG GGCCAGCCCC AGGAGAGGCC TCCCAGGGCT CAGACTCAGA GGGCGGTGCC 
CAGGGCCTGG CCTCAGCTAG CAGGGACCAC ACAGACCAGA CTTCGGAGAC CGCACCAGAC 
GCATCGGTGC CACCCAGTGT GAAACCAAAG AAGCGGAGAA AGAGCTACCG GAGCTTCTTA 
CCTGAGAAGA GTGGCTACCC TGACATCGGC TTCCCCCTCT TCCCACTTTC CAAGGGTTTC 
ATCAAGAGCA TCCGGGGTGT CCTGACGCAG TTCCGGGCTG CCCTGCTAGA GGCAGGCATG 
CCGGAGTGCA CAGAGGACAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

UBE2O is a quality control factor for orphans of multiprotein complexes.
Science (New York, N.Y.)357(6350)472-475(2017 08)
Yanagitani K,Juszkiewicz S,Hegde RS

The ubiquitin-conjugating enzyme UBE2O modulates c-Maf stability and induces myeloma cell apoptosis.
Journal of hematology & oncology10(1)132(2017 Jul)
Xu Y,Zhang Z,Li J,Tong J,Cao B,Taylor P,Tang X,Wu D,Moran MF,Zeng Y,Mao X

A UBE2O-AMPK?2 Axis that Promotes Tumor Initiation and Progression Offers Opportunities for Therapy.
Cancer cell31(2)208-224(2017 Feb)
Vila IK,Yao Y,Kim G,Xia W,Kim H,Kim SJ,Park MK,Hwang JP,Gonz?lez-Billalabeitia E,Hung MC,Song SJ,Song MS

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UBE2O cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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