BLM cDNA ORF clone, Homo sapiens(human)

The following BLM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BLM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu48483 XM_006720632.2
Latest version!
Homo sapiens BLM RecQ like helicase (BLM), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $391.30
$559.00
OHu56490 XM_011521881.2
Latest version!
Homo sapiens BLM RecQ like helicase (BLM), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $475.30
$679.00
OHu56491 XM_011521882.3
Latest version!
Homo sapiens BLM RecQ like helicase (BLM), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu20445 NM_000057.4
Latest version!
Homo sapiens BLM RecQ like helicase (BLM), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $727.30
$1039.00
OHu20445 NM_001287246.2
Latest version!
Homo sapiens BLM RecQ like helicase (BLM), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $727.30
$1039.00
OHu20947 NM_001287248.2
Latest version!
Homo sapiens BLM RecQ like helicase (BLM), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $559.30
$799.00
OHu20391 NM_001287247.2
Latest version!
Homo sapiens BLM RecQ like helicase (BLM), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
View Old Accession Versions >>
OHu56491 XM_011521882.2 Homo sapiens Bloom syndrome RecQ like helicase (BLM), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu20445 NM_001287246.1 Homo sapiens BLM RecQ like helicase (BLM), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $727.30
$1039.00
OHu20391 NM_001287247.1 Homo sapiens BLM RecQ like helicase (BLM), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu20445 NM_000057.3 Homo sapiens Bloom syndrome RecQ like helicase (BLM), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $727.30
$1039.00
OHu20947 NM_001287248.1 Homo sapiens BLM RecQ like helicase (BLM), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $559.30
$799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu48483
    Clone ID Related Accession (Same CDS sequence) XM_006720632.2
    Accession Version XM_006720632.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2292bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product Bloom syndrome protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006720632.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGATGAAGA TTTTTCATAA AAAATTTGGC CTGCATAATT TTAGAACTAA TCAGCTAGAG 
    GCGATCAATG CTGCACTGCT TGGTGAAGAC TGTTTTATCC TGATGCCGAC TGGAGGTGGT
    AAGAGTTTGT GTTACCAGCT CCCTGCCTGT GTTTCTCCTG GGGTCACTGT TGTCATTTCT
    CCCTTGAGAT CACTTATCGT AGATCAAGTC CAAAAGCTGA CTTCCTTGGA TATTCCAGCT
    ACATATCTGA CAGGTGATAA GACTGACTCA GAAGCTACAA ATATTTACCT CCAGTTATCA
    AAAAAAGACC CAATCATAAA ACTTCTATAT GTCACTCCAG AAAAGATCTG TGCAAGTAAC
    AGACTCATTT CTACTCTGGA GAATCTCTAT GAGAGGAAGC TCTTGGCACG TTTTGTTATT
    GATGAAGCAC ATTGTGTCAG TCAGTGGGGA CATGATTTTC GTCAAGATTA CAAAAGAATG
    AATATGCTTC GCCAGAAGTT TCCTTCTGTT CCGGTGATGG CTCTTACGGC CACAGCTAAT
    CCCAGGGTAC AGAAGGACAT CCTGACTCAG CTGAAGATTC TCAGACCTCA GGTGTTTAGC
    ATGAGCTTTA ACAGACATAA TCTGAAATAC TATGTATTAC CGAAAAAGCC TAAAAAGGTG
    GCATTTGATT GCCTAGAATG GATCAGAAAG CACCACCCAT ATGATTCAGG GATAATTTAC
    TGCCTCTCCA GGCGAGAATG TGACACCATG GCTGACACGT TACAGAGAGA TGGGCTCGCT
    GCTCTTGCTT ACCATGCTGG CCTCAGTGAT TCTGCCAGAG ATGAAGTGCA GCAGAAGTGG
    ATTAATCAGG ATGGCTGTCA GGTTATCTGT GCTACAATTG CATTTGGAAT GGGGATTGAC
    AAACCGGACG TGCGATTTGT GATTCATGCA TCTCTCCCTA AATCTGTGGA GGGTTACTAC
    CAAGAATCTG GCAGAGCTGG AAGAGATGGG GAAATATCTC ACTGCCTGCT TTTCTATACC
    TATCATGATG TGACCAGACT GAAAAGACTT ATAATGATGG AAAAAGATGG AAACCATCAT
    ACAAGAGAAA CTCACTTCAA TAATTTGTAT AGCATGGTAC ATTACTGTGA AAATATAACG
    GAATGCAGGA GAATACAGCT TTTGGCCTAC TTTGGTGAAA ATGGATTTAA TCCTGATTTT
    TGTAAGAAAC ACCCAGATGT TTCTTGTGAT AATTGCTGTA AAACAAAGGA TTATAAAACA
    AGAGATGTGA CTGACGATGT GAAAAGTATT GTAAGATTTG TTCAAGAACA TAGTTCATCA
    CAAGGAATGA GAAATATAAA ACATGTAGGT CCTTCTGGAA GATTTACTAT GAATATGCTG
    GTCGACATTT TCTTGGGGAG TAAGAGTGCA AAAATCCAGT CAGGTATATT TGGAAAAGGA
    TCTGCTTATT CACGACACAA TGCCGAAAGA CTTTTTAAAA AGCTGATACT TGACAAGATT
    TTGGATGAAG ACTTATATAT CAATGCCAAT GACCAGGCGA TCGCTTATGT GATGCTCGGA
    AATAAAGCCC AAACTGTACT AAATGGCAAT TTAAAGGTAG ACTTTATGGA AACAGAAAAT
    TCCAGCAGTG TGAAAAAACA AAAAGCGTTA GTAGCAAAAG TGTCTCAGAG GGAAGAGATG
    GTTAAAAAAT GTCTTGGAGA ACTTACAGAA GTCTGCAAAT CTCTGGGGAA AGTTTTTGGT
    GTCCATTACT TCAATATTTT TAATACCGTC ACTCTCAAGA AGCTTGCAGA ATCTTTATCT
    TCTGATCCTG AGGTTTTGCT TCAAATTGAT GGTGTTACTG AAGACAAACT GGAAAAATAT
    GGTGCGGAAG TGATTTCAGT ATTACAGAAA TACTCTGAAT GGACATCGCC AGCTGAAGAC
    AGTTCCCCAG GGATAAGCCT GTCCAGCAGC AGAGGCCCCG GAAGAAGTGC CGCTGAGGAG
    CTCGACGAGG AAATACCCGT ATCTTCCCAC TACTTTGCAA GTAAAACCAG AAATGAAAGG
    AAGAGGAAAA AGATGCCAGC CTCCCAAAGG TCTAAGAGGA GAAAAACTGC TTCCAGTGGT
    TCCAAGGCAA AGGGGGGGTC TGCCACATGT AGAAAGATAT CTTCCAAAAC GAAATCCTCC
    AGCATCATTG GATCCAGTTC AGCCTCACAT ACTTCTCAAG CGACATCAGG AGCCAATAGC
    AAATTGGGGA TTATGGCTCC ACCGAAGCCT ATAAATAGAC CGTTTCTTAA GCCTTCATAT
    GCATTCTCAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006720695.1
    CDS140..2431
    Translation

    Target ORF information:

    RefSeq Version XM_006720632.2
    Organism Homo sapiens(human)
    Definition Homo sapiens BLM RecQ like helicase (BLM), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006720632.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGATGAAGA TTTTTCATAA AAAATTTGGC CTGCATAATT TTAGAACTAA TCAGCTAGAG 
    GCGATCAATG CTGCACTGCT TGGTGAAGAC TGTTTTATCC TGATGCCGAC TGGAGGTGGT
    AAGAGTTTGT GTTACCAGCT CCCTGCCTGT GTTTCTCCTG GGGTCACTGT TGTCATTTCT
    CCCTTGAGAT CACTTATCGT AGATCAAGTC CAAAAGCTGA CTTCCTTGGA TATTCCAGCT
    ACATATCTGA CAGGTGATAA GACTGACTCA GAAGCTACAA ATATTTACCT CCAGTTATCA
    AAAAAAGACC CAATCATAAA ACTTCTATAT GTCACTCCAG AAAAGATCTG TGCAAGTAAC
    AGACTCATTT CTACTCTGGA GAATCTCTAT GAGAGGAAGC TCTTGGCACG TTTTGTTATT
    GATGAAGCAC ATTGTGTCAG TCAGTGGGGA CATGATTTTC GTCAAGATTA CAAAAGAATG
    AATATGCTTC GCCAGAAGTT TCCTTCTGTT CCGGTGATGG CTCTTACGGC CACAGCTAAT
    CCCAGGGTAC AGAAGGACAT CCTGACTCAG CTGAAGATTC TCAGACCTCA GGTGTTTAGC
    ATGAGCTTTA ACAGACATAA TCTGAAATAC TATGTATTAC CGAAAAAGCC TAAAAAGGTG
    GCATTTGATT GCCTAGAATG GATCAGAAAG CACCACCCAT ATGATTCAGG GATAATTTAC
    TGCCTCTCCA GGCGAGAATG TGACACCATG GCTGACACGT TACAGAGAGA TGGGCTCGCT
    GCTCTTGCTT ACCATGCTGG CCTCAGTGAT TCTGCCAGAG ATGAAGTGCA GCAGAAGTGG
    ATTAATCAGG ATGGCTGTCA GGTTATCTGT GCTACAATTG CATTTGGAAT GGGGATTGAC
    AAACCGGACG TGCGATTTGT GATTCATGCA TCTCTCCCTA AATCTGTGGA GGGTTACTAC
    CAAGAATCTG GCAGAGCTGG AAGAGATGGG GAAATATCTC ACTGCCTGCT TTTCTATACC
    TATCATGATG TGACCAGACT GAAAAGACTT ATAATGATGG AAAAAGATGG AAACCATCAT
    ACAAGAGAAA CTCACTTCAA TAATTTGTAT AGCATGGTAC ATTACTGTGA AAATATAACG
    GAATGCAGGA GAATACAGCT TTTGGCCTAC TTTGGTGAAA ATGGATTTAA TCCTGATTTT
    TGTAAGAAAC ACCCAGATGT TTCTTGTGAT AATTGCTGTA AAACAAAGGA TTATAAAACA
    AGAGATGTGA CTGACGATGT GAAAAGTATT GTAAGATTTG TTCAAGAACA TAGTTCATCA
    CAAGGAATGA GAAATATAAA ACATGTAGGT CCTTCTGGAA GATTTACTAT GAATATGCTG
    GTCGACATTT TCTTGGGGAG TAAGAGTGCA AAAATCCAGT CAGGTATATT TGGAAAAGGA
    TCTGCTTATT CACGACACAA TGCCGAAAGA CTTTTTAAAA AGCTGATACT TGACAAGATT
    TTGGATGAAG ACTTATATAT CAATGCCAAT GACCAGGCGA TCGCTTATGT GATGCTCGGA
    AATAAAGCCC AAACTGTACT AAATGGCAAT TTAAAGGTAG ACTTTATGGA AACAGAAAAT
    TCCAGCAGTG TGAAAAAACA AAAAGCGTTA GTAGCAAAAG TGTCTCAGAG GGAAGAGATG
    GTTAAAAAAT GTCTTGGAGA ACTTACAGAA GTCTGCAAAT CTCTGGGGAA AGTTTTTGGT
    GTCCATTACT TCAATATTTT TAATACCGTC ACTCTCAAGA AGCTTGCAGA ATCTTTATCT
    TCTGATCCTG AGGTTTTGCT TCAAATTGAT GGTGTTACTG AAGACAAACT GGAAAAATAT
    GGTGCGGAAG TGATTTCAGT ATTACAGAAA TACTCTGAAT GGACATCGCC AGCTGAAGAC
    AGTTCCCCAG GGATAAGCCT GTCCAGCAGC AGAGGCCCCG GAAGAAGTGC CGCTGAGGAG
    CTCGACGAGG AAATACCCGT ATCTTCCCAC TACTTTGCAA GTAAAACCAG AAATGAAAGG
    AAGAGGAAAA AGATGCCAGC CTCCCAAAGG TCTAAGAGGA GAAAAACTGC TTCCAGTGGT
    TCCAAGGCAA AGGGGGGGTC TGCCACATGT AGAAAGATAT CTTCCAAAAC GAAATCCTCC
    AGCATCATTG GATCCAGTTC AGCCTCACAT ACTTCTCAAG CGACATCAGG AGCCAATAGC
    AAATTGGGGA TTATGGCTCC ACCGAAGCCT ATAAATAGAC CGTTTCTTAA GCCTTCATAT
    GCATTCTCAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56490
    Clone ID Related Accession (Same CDS sequence) XM_011521881.2
    Accession Version XM_011521881.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2940bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product Bloom syndrome protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521881.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGAGGGTG ATTCCTGCCC TACAGGGAAT TCTATGAAGG AGTTAAATTT TTCACACCTT 
    CCCTCAAATT CTGTTTCTCC TGGGGACTGT TTACTGACTA CCACCCTAGG AAAGACAGGA
    TTCTCTGCCA CCAGGAAGAA TCTTTTTGAA AGGCCTTTAT TCAATACCCA TTTACAGAAG
    TCCTTTGTAA GTAGCAACTG GGCTGAAACA CCAAGACTAG GAAAAAAAAA TGAAAGCTCT
    TATTTCCCAG GAAATGTTCT CACAAGCACT GCTGTGAAAG ATCAGAATAA ACATACTGCT
    TCAATAAATG ACTTAGAAAG AGAAACCCAA CCTTCCTATG ATATTGATAA TTTTGACATA
    GATGACTTTG ATGATGATGA TGACTGGGAA GACATAATGC ATAATTTAGC AGCCAGCAAA
    TCTTCCACAG CTGCCTATCA ACCCATCAAG GAAGGTCGGC CAATTAAATC AGTATCAGAA
    AGACTTTCCT CAGCCAAGAC AGACTGTCTT CCAGTGTCAT CTACTGCTCA AAATATAAAC
    TTCTCAGAGT CAATTCAGAA TTATACTGAC AAGTCAGCAC AAAATTTAGC ATCCAGAAAT
    CTGAAACATG AGCGTTTCCA AAGTCTTAGT TTTCCTCATA CAAAGGAAAT GATGAAGATT
    TTTCATAAAA AATTTGGCCT GCATAATTTT AGAACTAATC AGCTAGAGGC GATCAATGCT
    GCACTGCTTG GTGAAGACTG TTTTATCCTG ATGCCGACTG GAGGTGGTAA GAGTTTGTGT
    TACCAGCTCC CTGCCTGTGT TTCTCCTGGG GTCACTGTTG TCATTTCTCC CTTGAGATCA
    CTTATCGTAG ATCAAGTCCA AAAGCTGACT TCCTTGGATA TTCCAGCTAC ATATCTGACA
    GGTGATAAGA CTGACTCAGA AGCTACAAAT ATTTACCTCC AGTTATCAAA AAAAGACCCA
    ATCATAAAAC TTCTATATGT CACTCCAGAA AAGATCTGTG CAAGTAACAG ACTCATTTCT
    ACTCTGGAGA ATCTCTATGA GAGGAAGCTC TTGGCACGTT TTGTTATTGA TGAAGCACAT
    TGTGTCAGTC AGTGGGGACA TGATTTTCGT CAAGATTACA AAAGAATGAA TATGCTTCGC
    CAGAAGTTTC CTTCTGTTCC GGTGATGGCT CTTACGGCCA CAGCTAATCC CAGGGTACAG
    AAGGACATCC TGACTCAGCT GAAGATTCTC AGACCTCAGG TGTTTAGCAT GAGCTTTAAC
    AGACATAATC TGAAATACTA TGTATTACCG AAAAAGCCTA AAAAGGTGGC ATTTGATTGC
    CTAGAATGGA TCAGAAAGCA CCACCCATAT GATTCAGGGA TAATTTACTG CCTCTCCAGG
    CGAGAATGTG ACACCATGGC TGACACGTTA CAGAGAGATG GGCTCGCTGC TCTTGCTTAC
    CATGCTGGCC TCAGTGATTC TGCCAGAGAT GAAGTGCAGC AGAAGTGGAT TAATCAGGAT
    GGCTGTCAGG TTATCTGTGC TACAATTGCA TTTGGAATGG GGATTGACAA ACCGGACGTG
    CGATTTGTGA TTCATGCATC TCTCCCTAAA TCTGTGGAGG GTTACTACCA AGAATCTGGC
    AGAGCTGGAA GAGATGGGGA AATATCTCAC TGCCTGCTTT TCTATACCTA TCATGATGTG
    ACCAGACTGA AAAGACTTAT AATGATGGAA AAAGATGGAA ACCATCATAC AAGAGAAACT
    CACTTCAATA ATTTGTATAG CATGGTACAT TACTGTGAAA ATATAACGGA ATGCAGGAGA
    ATACAGCTTT TGGCCTACTT TGGTGAAAAT GGATTTAATC CTGATTTTTG TAAGAAACAC
    CCAGATGTTT CTTGTGATAA TTGCTGTAAA ACAAAGGATT ATAAAACAAG AGATGTGACT
    GACGATGTGA AAAGTATTGT AAGATTTGTT CAAGAACATA GTTCATCACA AGGAATGAGA
    AATATAAAAC ATGTAGGTCC TTCTGGAAGA TTTACTATGA ATATGCTGGT CGACATTTTC
    TTGGGGAGTA AGAGTGCAAA AATCCAGTCA GGTATATTTG GAAAAGGATC TGCTTATTCA
    CGACACAATG CCGAAAGACT TTTTAAAAAG CTGATACTTG ACAAGATTTT GGATGAAGAC
    TTATATATCA ATGCCAATGA CCAGGCGATC GCTTATGTGA TGCTCGGAAA TAAAGCCCAA
    ACTGTACTAA ATGGCAATTT AAAGGTAGAC TTTATGGAAA CAGAAAATTC CAGCAGTGTG
    AAAAAACAAA AAGCGTTAGT AGCAAAAGTG TCTCAGAGGG AAGAGATGGT TAAAAAATGT
    CTTGGAGAAC TTACAGAAGT CTGCAAATCT CTGGGGAAAG TTTTTGGTGT CCATTACTTC
    AATATTTTTA ATACCGTCAC TCTCAAGAAG CTTGCAGAAT CTTTATCTTC TGATCCTGAG
    GTTTTGCTTC AAATTGATGG TGTTACTGAA GACAAACTGG AAAAATATGG TGCGGAAGTG
    ATTTCAGTAT TACAGAAATA CTCTGAATGG ACATCGCCAG CTGAAGACAG TTCCCCAGGG
    ATAAGCCTGT CCAGCAGCAG AGGCCCCGGA AGAAGTGCCG CTGAGGAGCT CGACGAGGAA
    ATACCCGTAT CTTCCCACTA CTTTGCAAGT AAAACCAGAA ATGAAAGGAA GAGGAAAAAG
    ATGCCAGCCT CCCAAAGGTC TAAGAGGAGA AAAACTGCTT CCAGTGGTTC CAAGGCAAAG
    GGGGGGTCTG CCACATGTAG AAAGATATCT TCCAAAACGA AATCCTCCAG CATCATTGGA
    TCCAGTTCAG CCTCACATAC TTCTCAAGCG ACATCAGGAG CCAATAGCAA ATTGGGGATT
    ATGGCTCCAC CGAAGCCTAT AAATAGACCG TTTCTTAAGC CTTCATATGC ATTCTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011520183.1
    CDS468..3407
    Translation

    Target ORF information:

    RefSeq Version XM_011521881.2
    Organism Homo sapiens(human)
    Definition Homo sapiens BLM RecQ like helicase (BLM), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011521881.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGAGGGTG ATTCCTGCCC TACAGGGAAT TCTATGAAGG AGTTAAATTT TTCACACCTT 
    CCCTCAAATT CTGTTTCTCC TGGGGACTGT TTACTGACTA CCACCCTAGG AAAGACAGGA
    TTCTCTGCCA CCAGGAAGAA TCTTTTTGAA AGGCCTTTAT TCAATACCCA TTTACAGAAG
    TCCTTTGTAA GTAGCAACTG GGCTGAAACA CCAAGACTAG GAAAAAAAAA TGAAAGCTCT
    TATTTCCCAG GAAATGTTCT CACAAGCACT GCTGTGAAAG ATCAGAATAA ACATACTGCT
    TCAATAAATG ACTTAGAAAG AGAAACCCAA CCTTCCTATG ATATTGATAA TTTTGACATA
    GATGACTTTG ATGATGATGA TGACTGGGAA GACATAATGC ATAATTTAGC AGCCAGCAAA
    TCTTCCACAG CTGCCTATCA ACCCATCAAG GAAGGTCGGC CAATTAAATC AGTATCAGAA
    AGACTTTCCT CAGCCAAGAC AGACTGTCTT CCAGTGTCAT CTACTGCTCA AAATATAAAC
    TTCTCAGAGT CAATTCAGAA TTATACTGAC AAGTCAGCAC AAAATTTAGC ATCCAGAAAT
    CTGAAACATG AGCGTTTCCA AAGTCTTAGT TTTCCTCATA CAAAGGAAAT GATGAAGATT
    TTTCATAAAA AATTTGGCCT GCATAATTTT AGAACTAATC AGCTAGAGGC GATCAATGCT
    GCACTGCTTG GTGAAGACTG TTTTATCCTG ATGCCGACTG GAGGTGGTAA GAGTTTGTGT
    TACCAGCTCC CTGCCTGTGT TTCTCCTGGG GTCACTGTTG TCATTTCTCC CTTGAGATCA
    CTTATCGTAG ATCAAGTCCA AAAGCTGACT TCCTTGGATA TTCCAGCTAC ATATCTGACA
    GGTGATAAGA CTGACTCAGA AGCTACAAAT ATTTACCTCC AGTTATCAAA AAAAGACCCA
    ATCATAAAAC TTCTATATGT CACTCCAGAA AAGATCTGTG CAAGTAACAG ACTCATTTCT
    ACTCTGGAGA ATCTCTATGA GAGGAAGCTC TTGGCACGTT TTGTTATTGA TGAAGCACAT
    TGTGTCAGTC AGTGGGGACA TGATTTTCGT CAAGATTACA AAAGAATGAA TATGCTTCGC
    CAGAAGTTTC CTTCTGTTCC GGTGATGGCT CTTACGGCCA CAGCTAATCC CAGGGTACAG
    AAGGACATCC TGACTCAGCT GAAGATTCTC AGACCTCAGG TGTTTAGCAT GAGCTTTAAC
    AGACATAATC TGAAATACTA TGTATTACCG AAAAAGCCTA AAAAGGTGGC ATTTGATTGC
    CTAGAATGGA TCAGAAAGCA CCACCCATAT GATTCAGGGA TAATTTACTG CCTCTCCAGG
    CGAGAATGTG ACACCATGGC TGACACGTTA CAGAGAGATG GGCTCGCTGC TCTTGCTTAC
    CATGCTGGCC TCAGTGATTC TGCCAGAGAT GAAGTGCAGC AGAAGTGGAT TAATCAGGAT
    GGCTGTCAGG TTATCTGTGC TACAATTGCA TTTGGAATGG GGATTGACAA ACCGGACGTG
    CGATTTGTGA TTCATGCATC TCTCCCTAAA TCTGTGGAGG GTTACTACCA AGAATCTGGC
    AGAGCTGGAA GAGATGGGGA AATATCTCAC TGCCTGCTTT TCTATACCTA TCATGATGTG
    ACCAGACTGA AAAGACTTAT AATGATGGAA AAAGATGGAA ACCATCATAC AAGAGAAACT
    CACTTCAATA ATTTGTATAG CATGGTACAT TACTGTGAAA ATATAACGGA ATGCAGGAGA
    ATACAGCTTT TGGCCTACTT TGGTGAAAAT GGATTTAATC CTGATTTTTG TAAGAAACAC
    CCAGATGTTT CTTGTGATAA TTGCTGTAAA ACAAAGGATT ATAAAACAAG AGATGTGACT
    GACGATGTGA AAAGTATTGT AAGATTTGTT CAAGAACATA GTTCATCACA AGGAATGAGA
    AATATAAAAC ATGTAGGTCC TTCTGGAAGA TTTACTATGA ATATGCTGGT CGACATTTTC
    TTGGGGAGTA AGAGTGCAAA AATCCAGTCA GGTATATTTG GAAAAGGATC TGCTTATTCA
    CGACACAATG CCGAAAGACT TTTTAAAAAG CTGATACTTG ACAAGATTTT GGATGAAGAC
    TTATATATCA ATGCCAATGA CCAGGCGATC GCTTATGTGA TGCTCGGAAA TAAAGCCCAA
    ACTGTACTAA ATGGCAATTT AAAGGTAGAC TTTATGGAAA CAGAAAATTC CAGCAGTGTG
    AAAAAACAAA AAGCGTTAGT AGCAAAAGTG TCTCAGAGGG AAGAGATGGT TAAAAAATGT
    CTTGGAGAAC TTACAGAAGT CTGCAAATCT CTGGGGAAAG TTTTTGGTGT CCATTACTTC
    AATATTTTTA ATACCGTCAC TCTCAAGAAG CTTGCAGAAT CTTTATCTTC TGATCCTGAG
    GTTTTGCTTC AAATTGATGG TGTTACTGAA GACAAACTGG AAAAATATGG TGCGGAAGTG
    ATTTCAGTAT TACAGAAATA CTCTGAATGG ACATCGCCAG CTGAAGACAG TTCCCCAGGG
    ATAAGCCTGT CCAGCAGCAG AGGCCCCGGA AGAAGTGCCG CTGAGGAGCT CGACGAGGAA
    ATACCCGTAT CTTCCCACTA CTTTGCAAGT AAAACCAGAA ATGAAAGGAA GAGGAAAAAG
    ATGCCAGCCT CCCAAAGGTC TAAGAGGAGA AAAACTGCTT CCAGTGGTTC CAAGGCAAAG
    GGGGGGTCTG CCACATGTAG AAAGATATCT TCCAAAACGA AATCCTCCAG CATCATTGGA
    TCCAGTTCAG CCTCACATAC TTCTCAAGCG ACATCAGGAG CCAATAGCAA ATTGGGGATT
    ATGGCTCCAC CGAAGCCTAT AAATAGACCG TTTCTTAAGC CTTCATATGC ATTCTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56491
    Clone ID Related Accession (Same CDS sequence) XM_011521882.2 , XM_011521882.3
    Accession Version XM_011521882.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2724bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product Bloom syndrome protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521882.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
    CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA
    ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA
    AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC
    ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG
    AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC
    ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA
    TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT
    GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA
    AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA
    ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG
    ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT
    GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT
    CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA
    CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT
    GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT
    ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT
    CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC
    TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT
    AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG
    CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT
    GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG
    GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA
    AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT
    GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT
    GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC
    CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA
    AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC
    TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC
    ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT
    TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA
    GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA
    CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT
    AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG
    CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG
    CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC
    GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT
    AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA
    AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG
    GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC
    AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG
    TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC
    ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTAATTG TACAACCATC CCCAATATCT
    AAGTTTACAG CATTTTTATC ACCCCAGCGA GAAACCTGGT ACCCATTAGC AGCTGCTCCG
    CATCTTCTCC CTCTTGTTTG TGCCCGGCTT CTTTCACTGA GCGTAGTTTT CAAGGGTCAT
    CCATGTTGTA GCATTGGTCA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011520184.1
    CDS106..2829
    Translation

    Target ORF information:

    RefSeq Version XM_011521882.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Bloom syndrome RecQ like helicase (BLM), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011521882.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
    CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA
    ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA
    AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC
    ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG
    AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC
    ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA
    TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT
    GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA
    AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA
    ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG
    ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT
    GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT
    CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA
    CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT
    GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT
    ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT
    CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC
    TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT
    AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG
    CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT
    GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG
    GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA
    AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT
    GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT
    GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC
    CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA
    AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC
    TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC
    ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT
    TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA
    GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA
    CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT
    AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG
    CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG
    CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC
    GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT
    AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA
    AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG
    GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC
    AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG
    TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC
    ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTAATTG TACAACCATC CCCAATATCT
    AAGTTTACAG CATTTTTATC ACCCCAGCGA GAAACCTGGT ACCCATTAGC AGCTGCTCCG
    CATCTTCTCC CTCTTGTTTG TGCCCGGCTT CTTTCACTGA GCGTAGTTTT CAAGGGTCAT
    CCATGTTGTA GCATTGGTCA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56491
    Clone ID Related Accession (Same CDS sequence) XM_011521882.2 , XM_011521882.3
    Accession Version XM_011521882.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2724bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product Bloom syndrome protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011521882.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
    CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA
    ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA
    AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC
    ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG
    AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC
    ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA
    TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT
    GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA
    AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA
    ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG
    ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT
    GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT
    CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA
    CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT
    GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT
    ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT
    CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC
    TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT
    AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG
    CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT
    GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG
    GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA
    AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT
    GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT
    GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC
    CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA
    AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC
    TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC
    ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT
    TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA
    GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA
    CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT
    AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG
    CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG
    CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC
    GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT
    AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA
    AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG
    GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC
    AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG
    TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC
    ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTAATTG TACAACCATC CCCAATATCT
    AAGTTTACAG CATTTTTATC ACCCCAGCGA GAAACCTGGT ACCCATTAGC AGCTGCTCCG
    CATCTTCTCC CTCTTGTTTG TGCCCGGCTT CTTTCACTGA GCGTAGTTTT CAAGGGTCAT
    CCATGTTGTA GCATTGGTCA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011520184.1
    CDS48..2771
    Translation

    Target ORF information:

    RefSeq Version XM_011521882.3
    Organism Homo sapiens(human)
    Definition Homo sapiens BLM RecQ like helicase (BLM), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011521882.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
    CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA
    ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA
    AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC
    ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG
    AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC
    ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA
    TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT
    GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA
    AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA
    ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG
    ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT
    GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT
    CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA
    CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT
    GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT
    ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT
    CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC
    TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT
    AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG
    CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT
    GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG
    GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA
    AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT
    GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT
    GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC
    CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA
    AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC
    TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC
    ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT
    TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA
    GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA
    CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT
    AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG
    CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG
    CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC
    GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT
    AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA
    AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG
    GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC
    AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG
    TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC
    ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTAATTG TACAACCATC CCCAATATCT
    AAGTTTACAG CATTTTTATC ACCCCAGCGA GAAACCTGGT ACCCATTAGC AGCTGCTCCG
    CATCTTCTCC CTCTTGTTTG TGCCCGGCTT CTTTCACTGA GCGTAGTTTT CAAGGGTCAT
    CCATGTTGTA GCATTGGTCA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20445
    Clone ID Related Accession (Same CDS sequence) NM_001287246.1 , NM_001287246.2 , NM_000057.4 , NM_000057.3
    Accession Version NM_001287246.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4254bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product Bloom syndrome protein isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD707743.1, BP363946.1, BC143288.1, BC115032.1, DB520927.1 and AC124248.6. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143288.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
    CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA
    ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA
    AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC
    ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG
    AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC
    ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA
    TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT
    GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA
    AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA
    ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG
    ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT
    GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT
    CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA
    CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT
    GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT
    ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT
    CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC
    TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT
    AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG
    CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT
    GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG
    GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA
    AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT
    GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT
    GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC
    CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA
    AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC
    TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC
    ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT
    TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA
    GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA
    CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT
    AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG
    CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG
    CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC
    GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT
    AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA
    AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG
    GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC
    AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG
    TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC
    ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTGTTTA GCATGAGCTT TAACAGACAT
    AATCTGAAAT ACTATGTATT ACCGAAAAAG CCTAAAAAGG TGGCATTTGA TTGCCTAGAA
    TGGATCAGAA AGCACCACCC ATATGATTCA GGGATAATTT ACTGCCTCTC CAGGCGAGAA
    TGTGACACCA TGGCTGACAC GTTACAGAGA GATGGGCTCG CTGCTCTTGC TTACCATGCT
    GGCCTCAGTG ATTCTGCCAG AGATGAAGTG CAGCAGAAGT GGATTAATCA GGATGGCTGT
    CAGGTTATCT GTGCTACAAT TGCATTTGGA ATGGGGATTG ACAAACCGGA CGTGCGATTT
    GTGATTCATG CATCTCTCCC TAAATCTGTG GAGGGTTACT ACCAAGAATC TGGCAGAGCT
    GGAAGAGATG GGGAAATATC TCACTGCCTG CTTTTCTATA CCTATCATGA TGTGACCAGA
    CTGAAAAGAC TTATAATGAT GGAAAAAGAT GGAAACCATC ATACAAGAGA AACTCACTTC
    AATAATTTGT ATAGCATGGT ACATTACTGT GAAAATATAA CGGAATGCAG GAGAATACAG
    CTTTTGGCCT ACTTTGGTGA AAATGGATTT AATCCTGATT TTTGTAAGAA ACACCCAGAT
    GTTTCTTGTG ATAATTGCTG TAAAACAAAG GATTATAAAA CAAGAGATGT GACTGACGAT
    GTGAAAAGTA TTGTAAGATT TGTTCAAGAA CATAGTTCAT CACAAGGAAT GAGAAATATA
    AAACATGTAG GTCCTTCTGG AAGATTTACT ATGAATATGC TGGTCGACAT TTTCTTGGGG
    AGTAAGAGTG CAAAAATCCA GTCAGGTATA TTTGGAAAAG GATCTGCTTA TTCACGACAC
    AATGCCGAAA GACTTTTTAA AAAGCTGATA CTTGACAAGA TTTTGGATGA AGACTTATAT
    ATCAATGCCA ATGACCAGGC GATCGCTTAT GTGATGCTCG GAAATAAAGC CCAAACTGTA
    CTAAATGGCA ATTTAAAGGT AGACTTTATG GAAACAGAAA ATTCCAGCAG TGTGAAAAAA
    CAAAAAGCGT TAGTAGCAAA AGTGTCTCAG AGGGAAGAGA TGGTTAAAAA ATGTCTTGGA
    GAACTTACAG AAGTCTGCAA ATCTCTGGGG AAAGTTTTTG GTGTCCATTA CTTCAATATT
    TTTAATACCG TCACTCTCAA GAAGCTTGCA GAATCTTTAT CTTCTGATCC TGAGGTTTTG
    CTTCAAATTG ATGGTGTTAC TGAAGACAAA CTGGAAAAAT ATGGTGCGGA AGTGATTTCA
    GTATTACAGA AATACTCTGA ATGGACATCG CCAGCTGAAG ACAGTTCCCC AGGGATAAGC
    CTGTCCAGCA GCAGAGGCCC CGGAAGAAGT GCCGCTGAGG AGCTCGACGA GGAAATACCC
    GTATCTTCCC ACTACTTTGC AAGTAAAACC AGAAATGAAA GGAAGAGGAA AAAGATGCCA
    GCCTCCCAAA GGTCTAAGAG GAGAAAAACT GCTTCCAGTG GTTCCAAGGC AAAGGGGGGG
    TCTGCCACAT GTAGAAAGAT ATCTTCCAAA ACGAAATCCT CCAGCATCAT TGGATCCAGT
    TCAGCCTCAC ATACTTCTCA AGCGACATCA GGAGCCAATA GCAAATTGGG GATTATGGCT
    CCACCGAAGC CTATAAATAG ACCGTTTCTT AAGCCTTCAT ATGCATTCTC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001274175.1
    CDS229..4482
    Translation

    Target ORF information:

    RefSeq Version NM_001287246.1
    Organism Homo sapiens(human)
    Definition Homo sapiens BLM RecQ like helicase (BLM), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001287246.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
    CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA
    ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA
    AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC
    ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG
    AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC
    ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA
    TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT
    GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA
    AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA
    ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG
    ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT
    GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT
    CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA
    CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT
    GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT
    ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT
    CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC
    TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT
    AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG
    CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT
    GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG
    GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA
    AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT
    GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT
    GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC
    CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA
    AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC
    TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC
    ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT
    TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA
    GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA
    CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT
    AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG
    CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG
    CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC
    GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT
    AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA
    AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG
    GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC
    AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG
    TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC
    ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTGTTTA GCATGAGCTT TAACAGACAT
    AATCTGAAAT ACTATGTATT ACCGAAAAAG CCTAAAAAGG TGGCATTTGA TTGCCTAGAA
    TGGATCAGAA AGCACCACCC ATATGATTCA GGGATAATTT ACTGCCTCTC CAGGCGAGAA
    TGTGACACCA TGGCTGACAC GTTACAGAGA GATGGGCTCG CTGCTCTTGC TTACCATGCT
    GGCCTCAGTG ATTCTGCCAG AGATGAAGTG CAGCAGAAGT GGATTAATCA GGATGGCTGT
    CAGGTTATCT GTGCTACAAT TGCATTTGGA ATGGGGATTG ACAAACCGGA CGTGCGATTT
    GTGATTCATG CATCTCTCCC TAAATCTGTG GAGGGTTACT ACCAAGAATC TGGCAGAGCT
    GGAAGAGATG GGGAAATATC TCACTGCCTG CTTTTCTATA CCTATCATGA TGTGACCAGA
    CTGAAAAGAC TTATAATGAT GGAAAAAGAT GGAAACCATC ATACAAGAGA AACTCACTTC
    AATAATTTGT ATAGCATGGT ACATTACTGT GAAAATATAA CGGAATGCAG GAGAATACAG
    CTTTTGGCCT ACTTTGGTGA AAATGGATTT AATCCTGATT TTTGTAAGAA ACACCCAGAT
    GTTTCTTGTG ATAATTGCTG TAAAACAAAG GATTATAAAA CAAGAGATGT GACTGACGAT
    GTGAAAAGTA TTGTAAGATT TGTTCAAGAA CATAGTTCAT CACAAGGAAT GAGAAATATA
    AAACATGTAG GTCCTTCTGG AAGATTTACT ATGAATATGC TGGTCGACAT TTTCTTGGGG
    AGTAAGAGTG CAAAAATCCA GTCAGGTATA TTTGGAAAAG GATCTGCTTA TTCACGACAC
    AATGCCGAAA GACTTTTTAA AAAGCTGATA CTTGACAAGA TTTTGGATGA AGACTTATAT
    ATCAATGCCA ATGACCAGGC GATCGCTTAT GTGATGCTCG GAAATAAAGC CCAAACTGTA
    CTAAATGGCA ATTTAAAGGT AGACTTTATG GAAACAGAAA ATTCCAGCAG TGTGAAAAAA
    CAAAAAGCGT TAGTAGCAAA AGTGTCTCAG AGGGAAGAGA TGGTTAAAAA ATGTCTTGGA
    GAACTTACAG AAGTCTGCAA ATCTCTGGGG AAAGTTTTTG GTGTCCATTA CTTCAATATT
    TTTAATACCG TCACTCTCAA GAAGCTTGCA GAATCTTTAT CTTCTGATCC TGAGGTTTTG
    CTTCAAATTG ATGGTGTTAC TGAAGACAAA CTGGAAAAAT ATGGTGCGGA AGTGATTTCA
    GTATTACAGA AATACTCTGA ATGGACATCG CCAGCTGAAG ACAGTTCCCC AGGGATAAGC
    CTGTCCAGCA GCAGAGGCCC CGGAAGAAGT GCCGCTGAGG AGCTCGACGA GGAAATACCC
    GTATCTTCCC ACTACTTTGC AAGTAAAACC AGAAATGAAA GGAAGAGGAA AAAGATGCCA
    GCCTCCCAAA GGTCTAAGAG GAGAAAAACT GCTTCCAGTG GTTCCAAGGC AAAGGGGGGG
    TCTGCCACAT GTAGAAAGAT ATCTTCCAAA ACGAAATCCT CCAGCATCAT TGGATCCAGT
    TCAGCCTCAC ATACTTCTCA AGCGACATCA GGAGCCAATA GCAAATTGGG GATTATGGCT
    CCACCGAAGC CTATAAATAG ACCGTTTCTT AAGCCTTCAT ATGCATTCTC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20445
    Clone ID Related Accession (Same CDS sequence) NM_001287246.1 , NM_001287246.2 , NM_000057.4 , NM_000057.3
    Accession Version NM_001287246.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4254bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product Bloom syndrome protein isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD707743.1, BP363946.1, BC143288.1, BC115032.1, DB520927.1 and AC124248.6. On May 31, 2019 this sequence version replaced NM_001287246.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143288.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
    CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA
    ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA
    AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC
    ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG
    AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC
    ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA
    TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT
    GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA
    AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA
    ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG
    ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT
    GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT
    CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA
    CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT
    GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT
    ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT
    CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC
    TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT
    AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG
    CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT
    GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG
    GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA
    AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT
    GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT
    GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC
    CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA
    AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC
    TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC
    ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT
    TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA
    GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA
    CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT
    AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG
    CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG
    CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC
    GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT
    AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA
    AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG
    GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC
    AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG
    TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC
    ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTGTTTA GCATGAGCTT TAACAGACAT
    AATCTGAAAT ACTATGTATT ACCGAAAAAG CCTAAAAAGG TGGCATTTGA TTGCCTAGAA
    TGGATCAGAA AGCACCACCC ATATGATTCA GGGATAATTT ACTGCCTCTC CAGGCGAGAA
    TGTGACACCA TGGCTGACAC GTTACAGAGA GATGGGCTCG CTGCTCTTGC TTACCATGCT
    GGCCTCAGTG ATTCTGCCAG AGATGAAGTG CAGCAGAAGT GGATTAATCA GGATGGCTGT
    CAGGTTATCT GTGCTACAAT TGCATTTGGA ATGGGGATTG ACAAACCGGA CGTGCGATTT
    GTGATTCATG CATCTCTCCC TAAATCTGTG GAGGGTTACT ACCAAGAATC TGGCAGAGCT
    GGAAGAGATG GGGAAATATC TCACTGCCTG CTTTTCTATA CCTATCATGA TGTGACCAGA
    CTGAAAAGAC TTATAATGAT GGAAAAAGAT GGAAACCATC ATACAAGAGA AACTCACTTC
    AATAATTTGT ATAGCATGGT ACATTACTGT GAAAATATAA CGGAATGCAG GAGAATACAG
    CTTTTGGCCT ACTTTGGTGA AAATGGATTT AATCCTGATT TTTGTAAGAA ACACCCAGAT
    GTTTCTTGTG ATAATTGCTG TAAAACAAAG GATTATAAAA CAAGAGATGT GACTGACGAT
    GTGAAAAGTA TTGTAAGATT TGTTCAAGAA CATAGTTCAT CACAAGGAAT GAGAAATATA
    AAACATGTAG GTCCTTCTGG AAGATTTACT ATGAATATGC TGGTCGACAT TTTCTTGGGG
    AGTAAGAGTG CAAAAATCCA GTCAGGTATA TTTGGAAAAG GATCTGCTTA TTCACGACAC
    AATGCCGAAA GACTTTTTAA AAAGCTGATA CTTGACAAGA TTTTGGATGA AGACTTATAT
    ATCAATGCCA ATGACCAGGC GATCGCTTAT GTGATGCTCG GAAATAAAGC CCAAACTGTA
    CTAAATGGCA ATTTAAAGGT AGACTTTATG GAAACAGAAA ATTCCAGCAG TGTGAAAAAA
    CAAAAAGCGT TAGTAGCAAA AGTGTCTCAG AGGGAAGAGA TGGTTAAAAA ATGTCTTGGA
    GAACTTACAG AAGTCTGCAA ATCTCTGGGG AAAGTTTTTG GTGTCCATTA CTTCAATATT
    TTTAATACCG TCACTCTCAA GAAGCTTGCA GAATCTTTAT CTTCTGATCC TGAGGTTTTG
    CTTCAAATTG ATGGTGTTAC TGAAGACAAA CTGGAAAAAT ATGGTGCGGA AGTGATTTCA
    GTATTACAGA AATACTCTGA ATGGACATCG CCAGCTGAAG ACAGTTCCCC AGGGATAAGC
    CTGTCCAGCA GCAGAGGCCC CGGAAGAAGT GCCGCTGAGG AGCTCGACGA GGAAATACCC
    GTATCTTCCC ACTACTTTGC AAGTAAAACC AGAAATGAAA GGAAGAGGAA AAAGATGCCA
    GCCTCCCAAA GGTCTAAGAG GAGAAAAACT GCTTCCAGTG GTTCCAAGGC AAAGGGGGGG
    TCTGCCACAT GTAGAAAGAT ATCTTCCAAA ACGAAATCCT CCAGCATCAT TGGATCCAGT
    TCAGCCTCAC ATACTTCTCA AGCGACATCA GGAGCCAATA GCAAATTGGG GATTATGGCT
    CCACCGAAGC CTATAAATAG ACCGTTTCTT AAGCCTTCAT ATGCATTCTC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001274175.1
    CDS210..4463
    Translation

    Target ORF information:

    RefSeq Version NM_001287246.2
    Organism Homo sapiens(human)
    Definition Homo sapiens BLM RecQ like helicase (BLM), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001287246.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
    CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA
    ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA
    AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC
    ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG
    AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC
    ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA
    TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT
    GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA
    AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA
    ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG
    ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT
    GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT
    CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA
    CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT
    GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT
    ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT
    CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC
    TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT
    AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG
    CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT
    GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG
    GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA
    AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT
    GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT
    GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC
    CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA
    AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC
    TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC
    ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT
    TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA
    GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA
    CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT
    AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG
    CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG
    CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC
    GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT
    AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA
    AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG
    GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC
    AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG
    TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC
    ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTGTTTA GCATGAGCTT TAACAGACAT
    AATCTGAAAT ACTATGTATT ACCGAAAAAG CCTAAAAAGG TGGCATTTGA TTGCCTAGAA
    TGGATCAGAA AGCACCACCC ATATGATTCA GGGATAATTT ACTGCCTCTC CAGGCGAGAA
    TGTGACACCA TGGCTGACAC GTTACAGAGA GATGGGCTCG CTGCTCTTGC TTACCATGCT
    GGCCTCAGTG ATTCTGCCAG AGATGAAGTG CAGCAGAAGT GGATTAATCA GGATGGCTGT
    CAGGTTATCT GTGCTACAAT TGCATTTGGA ATGGGGATTG ACAAACCGGA CGTGCGATTT
    GTGATTCATG CATCTCTCCC TAAATCTGTG GAGGGTTACT ACCAAGAATC TGGCAGAGCT
    GGAAGAGATG GGGAAATATC TCACTGCCTG CTTTTCTATA CCTATCATGA TGTGACCAGA
    CTGAAAAGAC TTATAATGAT GGAAAAAGAT GGAAACCATC ATACAAGAGA AACTCACTTC
    AATAATTTGT ATAGCATGGT ACATTACTGT GAAAATATAA CGGAATGCAG GAGAATACAG
    CTTTTGGCCT ACTTTGGTGA AAATGGATTT AATCCTGATT TTTGTAAGAA ACACCCAGAT
    GTTTCTTGTG ATAATTGCTG TAAAACAAAG GATTATAAAA CAAGAGATGT GACTGACGAT
    GTGAAAAGTA TTGTAAGATT TGTTCAAGAA CATAGTTCAT CACAAGGAAT GAGAAATATA
    AAACATGTAG GTCCTTCTGG AAGATTTACT ATGAATATGC TGGTCGACAT TTTCTTGGGG
    AGTAAGAGTG CAAAAATCCA GTCAGGTATA TTTGGAAAAG GATCTGCTTA TTCACGACAC
    AATGCCGAAA GACTTTTTAA AAAGCTGATA CTTGACAAGA TTTTGGATGA AGACTTATAT
    ATCAATGCCA ATGACCAGGC GATCGCTTAT GTGATGCTCG GAAATAAAGC CCAAACTGTA
    CTAAATGGCA ATTTAAAGGT AGACTTTATG GAAACAGAAA ATTCCAGCAG TGTGAAAAAA
    CAAAAAGCGT TAGTAGCAAA AGTGTCTCAG AGGGAAGAGA TGGTTAAAAA ATGTCTTGGA
    GAACTTACAG AAGTCTGCAA ATCTCTGGGG AAAGTTTTTG GTGTCCATTA CTTCAATATT
    TTTAATACCG TCACTCTCAA GAAGCTTGCA GAATCTTTAT CTTCTGATCC TGAGGTTTTG
    CTTCAAATTG ATGGTGTTAC TGAAGACAAA CTGGAAAAAT ATGGTGCGGA AGTGATTTCA
    GTATTACAGA AATACTCTGA ATGGACATCG CCAGCTGAAG ACAGTTCCCC AGGGATAAGC
    CTGTCCAGCA GCAGAGGCCC CGGAAGAAGT GCCGCTGAGG AGCTCGACGA GGAAATACCC
    GTATCTTCCC ACTACTTTGC AAGTAAAACC AGAAATGAAA GGAAGAGGAA AAAGATGCCA
    GCCTCCCAAA GGTCTAAGAG GAGAAAAACT GCTTCCAGTG GTTCCAAGGC AAAGGGGGGG
    TCTGCCACAT GTAGAAAGAT ATCTTCCAAA ACGAAATCCT CCAGCATCAT TGGATCCAGT
    TCAGCCTCAC ATACTTCTCA AGCGACATCA GGAGCCAATA GCAAATTGGG GATTATGGCT
    CCACCGAAGC CTATAAATAG ACCGTTTCTT AAGCCTTCAT ATGCATTCTC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20445
    Clone ID Related Accession (Same CDS sequence) NM_001287246.1 , NM_001287246.2 , NM_000057.4 , NM_000057.3
    Accession Version NM_000057.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4254bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-30
    Organism Homo sapiens(human)
    Product Bloom syndrome protein isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD707743.1, BC062697.1, U39817.1, BC115030.1, BC034480.1 and AC124248.6. On Nov 22, 2018 this sequence version replaced NM_000057.3. Transcript Variant: This variant (1) encodes the longest protein (isoform 1). Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC034480.1, U39817.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000355112.8/ ENSP00000347232.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
    CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA
    ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA
    AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC
    ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG
    AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC
    ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA
    TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT
    GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA
    AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA
    ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG
    ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT
    GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT
    CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA
    CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT
    GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT
    ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT
    CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC
    TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT
    AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG
    CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT
    GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG
    GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA
    AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT
    GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT
    GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC
    CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA
    AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC
    TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC
    ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT
    TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA
    GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA
    CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT
    AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG
    CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG
    CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC
    GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT
    AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA
    AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG
    GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC
    AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG
    TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC
    ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTGTTTA GCATGAGCTT TAACAGACAT
    AATCTGAAAT ACTATGTATT ACCGAAAAAG CCTAAAAAGG TGGCATTTGA TTGCCTAGAA
    TGGATCAGAA AGCACCACCC ATATGATTCA GGGATAATTT ACTGCCTCTC CAGGCGAGAA
    TGTGACACCA TGGCTGACAC GTTACAGAGA GATGGGCTCG CTGCTCTTGC TTACCATGCT
    GGCCTCAGTG ATTCTGCCAG AGATGAAGTG CAGCAGAAGT GGATTAATCA GGATGGCTGT
    CAGGTTATCT GTGCTACAAT TGCATTTGGA ATGGGGATTG ACAAACCGGA CGTGCGATTT
    GTGATTCATG CATCTCTCCC TAAATCTGTG GAGGGTTACT ACCAAGAATC TGGCAGAGCT
    GGAAGAGATG GGGAAATATC TCACTGCCTG CTTTTCTATA CCTATCATGA TGTGACCAGA
    CTGAAAAGAC TTATAATGAT GGAAAAAGAT GGAAACCATC ATACAAGAGA AACTCACTTC
    AATAATTTGT ATAGCATGGT ACATTACTGT GAAAATATAA CGGAATGCAG GAGAATACAG
    CTTTTGGCCT ACTTTGGTGA AAATGGATTT AATCCTGATT TTTGTAAGAA ACACCCAGAT
    GTTTCTTGTG ATAATTGCTG TAAAACAAAG GATTATAAAA CAAGAGATGT GACTGACGAT
    GTGAAAAGTA TTGTAAGATT TGTTCAAGAA CATAGTTCAT CACAAGGAAT GAGAAATATA
    AAACATGTAG GTCCTTCTGG AAGATTTACT ATGAATATGC TGGTCGACAT TTTCTTGGGG
    AGTAAGAGTG CAAAAATCCA GTCAGGTATA TTTGGAAAAG GATCTGCTTA TTCACGACAC
    AATGCCGAAA GACTTTTTAA AAAGCTGATA CTTGACAAGA TTTTGGATGA AGACTTATAT
    ATCAATGCCA ATGACCAGGC GATCGCTTAT GTGATGCTCG GAAATAAAGC CCAAACTGTA
    CTAAATGGCA ATTTAAAGGT AGACTTTATG GAAACAGAAA ATTCCAGCAG TGTGAAAAAA
    CAAAAAGCGT TAGTAGCAAA AGTGTCTCAG AGGGAAGAGA TGGTTAAAAA ATGTCTTGGA
    GAACTTACAG AAGTCTGCAA ATCTCTGGGG AAAGTTTTTG GTGTCCATTA CTTCAATATT
    TTTAATACCG TCACTCTCAA GAAGCTTGCA GAATCTTTAT CTTCTGATCC TGAGGTTTTG
    CTTCAAATTG ATGGTGTTAC TGAAGACAAA CTGGAAAAAT ATGGTGCGGA AGTGATTTCA
    GTATTACAGA AATACTCTGA ATGGACATCG CCAGCTGAAG ACAGTTCCCC AGGGATAAGC
    CTGTCCAGCA GCAGAGGCCC CGGAAGAAGT GCCGCTGAGG AGCTCGACGA GGAAATACCC
    GTATCTTCCC ACTACTTTGC AAGTAAAACC AGAAATGAAA GGAAGAGGAA AAAGATGCCA
    GCCTCCCAAA GGTCTAAGAG GAGAAAAACT GCTTCCAGTG GTTCCAAGGC AAAGGGGGGG
    TCTGCCACAT GTAGAAAGAT ATCTTCCAAA ACGAAATCCT CCAGCATCAT TGGATCCAGT
    TCAGCCTCAC ATACTTCTCA AGCGACATCA GGAGCCAATA GCAAATTGGG GATTATGGCT
    CCACCGAAGC CTATAAATAG ACCGTTTCTT AAGCCTTCAT ATGCATTCTC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000048.1
    CDS100..4353
    Translation

    Target ORF information:

    RefSeq Version NM_000057.4
    Organism Homo sapiens(human)
    Definition Homo sapiens BLM RecQ like helicase (BLM), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000057.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
    CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA
    ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA
    AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC
    ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG
    AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC
    ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA
    TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT
    GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA
    AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA
    ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG
    ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT
    GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT
    CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA
    CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT
    GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT
    ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT
    CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC
    TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT
    AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG
    CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT
    GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG
    GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA
    AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT
    GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT
    GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC
    CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA
    AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC
    TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC
    ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT
    TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA
    GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA
    CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT
    AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG
    CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG
    CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC
    GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT
    AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA
    AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG
    GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC
    AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG
    TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC
    ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTGTTTA GCATGAGCTT TAACAGACAT
    AATCTGAAAT ACTATGTATT ACCGAAAAAG CCTAAAAAGG TGGCATTTGA TTGCCTAGAA
    TGGATCAGAA AGCACCACCC ATATGATTCA GGGATAATTT ACTGCCTCTC CAGGCGAGAA
    TGTGACACCA TGGCTGACAC GTTACAGAGA GATGGGCTCG CTGCTCTTGC TTACCATGCT
    GGCCTCAGTG ATTCTGCCAG AGATGAAGTG CAGCAGAAGT GGATTAATCA GGATGGCTGT
    CAGGTTATCT GTGCTACAAT TGCATTTGGA ATGGGGATTG ACAAACCGGA CGTGCGATTT
    GTGATTCATG CATCTCTCCC TAAATCTGTG GAGGGTTACT ACCAAGAATC TGGCAGAGCT
    GGAAGAGATG GGGAAATATC TCACTGCCTG CTTTTCTATA CCTATCATGA TGTGACCAGA
    CTGAAAAGAC TTATAATGAT GGAAAAAGAT GGAAACCATC ATACAAGAGA AACTCACTTC
    AATAATTTGT ATAGCATGGT ACATTACTGT GAAAATATAA CGGAATGCAG GAGAATACAG
    CTTTTGGCCT ACTTTGGTGA AAATGGATTT AATCCTGATT TTTGTAAGAA ACACCCAGAT
    GTTTCTTGTG ATAATTGCTG TAAAACAAAG GATTATAAAA CAAGAGATGT GACTGACGAT
    GTGAAAAGTA TTGTAAGATT TGTTCAAGAA CATAGTTCAT CACAAGGAAT GAGAAATATA
    AAACATGTAG GTCCTTCTGG AAGATTTACT ATGAATATGC TGGTCGACAT TTTCTTGGGG
    AGTAAGAGTG CAAAAATCCA GTCAGGTATA TTTGGAAAAG GATCTGCTTA TTCACGACAC
    AATGCCGAAA GACTTTTTAA AAAGCTGATA CTTGACAAGA TTTTGGATGA AGACTTATAT
    ATCAATGCCA ATGACCAGGC GATCGCTTAT GTGATGCTCG GAAATAAAGC CCAAACTGTA
    CTAAATGGCA ATTTAAAGGT AGACTTTATG GAAACAGAAA ATTCCAGCAG TGTGAAAAAA
    CAAAAAGCGT TAGTAGCAAA AGTGTCTCAG AGGGAAGAGA TGGTTAAAAA ATGTCTTGGA
    GAACTTACAG AAGTCTGCAA ATCTCTGGGG AAAGTTTTTG GTGTCCATTA CTTCAATATT
    TTTAATACCG TCACTCTCAA GAAGCTTGCA GAATCTTTAT CTTCTGATCC TGAGGTTTTG
    CTTCAAATTG ATGGTGTTAC TGAAGACAAA CTGGAAAAAT ATGGTGCGGA AGTGATTTCA
    GTATTACAGA AATACTCTGA ATGGACATCG CCAGCTGAAG ACAGTTCCCC AGGGATAAGC
    CTGTCCAGCA GCAGAGGCCC CGGAAGAAGT GCCGCTGAGG AGCTCGACGA GGAAATACCC
    GTATCTTCCC ACTACTTTGC AAGTAAAACC AGAAATGAAA GGAAGAGGAA AAAGATGCCA
    GCCTCCCAAA GGTCTAAGAG GAGAAAAACT GCTTCCAGTG GTTCCAAGGC AAAGGGGGGG
    TCTGCCACAT GTAGAAAGAT ATCTTCCAAA ACGAAATCCT CCAGCATCAT TGGATCCAGT
    TCAGCCTCAC ATACTTCTCA AGCGACATCA GGAGCCAATA GCAAATTGGG GATTATGGCT
    CCACCGAAGC CTATAAATAG ACCGTTTCTT AAGCCTTCAT ATGCATTCTC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20445
    Clone ID Related Accession (Same CDS sequence) NM_001287246.1 , NM_001287246.2 , NM_000057.4 , NM_000057.3
    Accession Version NM_000057.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4254bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-10
    Organism Homo sapiens(human)
    Product Bloom syndrome protein isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD707743.1, BC062697.1, U39817.1, BC115030.1, BC034480.1 and AC124248.6. On Dec 11, 2013 this sequence version replaced NM_000057.2. Transcript Variant: This variant (1) encodes the longest protein (isoform 1). Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC034480.1, U39817.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
    CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA
    ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA
    AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC
    ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG
    AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC
    ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA
    TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT
    GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA
    AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA
    ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG
    ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT
    GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT
    CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA
    CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT
    GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT
    ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT
    CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC
    TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT
    AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG
    CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT
    GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG
    GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA
    AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT
    GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT
    GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC
    CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA
    AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC
    TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC
    ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT
    TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA
    GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA
    CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT
    AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG
    CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG
    CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC
    GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT
    AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA
    AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG
    GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC
    AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG
    TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC
    ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTGTTTA GCATGAGCTT TAACAGACAT
    AATCTGAAAT ACTATGTATT ACCGAAAAAG CCTAAAAAGG TGGCATTTGA TTGCCTAGAA
    TGGATCAGAA AGCACCACCC ATATGATTCA GGGATAATTT ACTGCCTCTC CAGGCGAGAA
    TGTGACACCA TGGCTGACAC GTTACAGAGA GATGGGCTCG CTGCTCTTGC TTACCATGCT
    GGCCTCAGTG ATTCTGCCAG AGATGAAGTG CAGCAGAAGT GGATTAATCA GGATGGCTGT
    CAGGTTATCT GTGCTACAAT TGCATTTGGA ATGGGGATTG ACAAACCGGA CGTGCGATTT
    GTGATTCATG CATCTCTCCC TAAATCTGTG GAGGGTTACT ACCAAGAATC TGGCAGAGCT
    GGAAGAGATG GGGAAATATC TCACTGCCTG CTTTTCTATA CCTATCATGA TGTGACCAGA
    CTGAAAAGAC TTATAATGAT GGAAAAAGAT GGAAACCATC ATACAAGAGA AACTCACTTC
    AATAATTTGT ATAGCATGGT ACATTACTGT GAAAATATAA CGGAATGCAG GAGAATACAG
    CTTTTGGCCT ACTTTGGTGA AAATGGATTT AATCCTGATT TTTGTAAGAA ACACCCAGAT
    GTTTCTTGTG ATAATTGCTG TAAAACAAAG GATTATAAAA CAAGAGATGT GACTGACGAT
    GTGAAAAGTA TTGTAAGATT TGTTCAAGAA CATAGTTCAT CACAAGGAAT GAGAAATATA
    AAACATGTAG GTCCTTCTGG AAGATTTACT ATGAATATGC TGGTCGACAT TTTCTTGGGG
    AGTAAGAGTG CAAAAATCCA GTCAGGTATA TTTGGAAAAG GATCTGCTTA TTCACGACAC
    AATGCCGAAA GACTTTTTAA AAAGCTGATA CTTGACAAGA TTTTGGATGA AGACTTATAT
    ATCAATGCCA ATGACCAGGC GATCGCTTAT GTGATGCTCG GAAATAAAGC CCAAACTGTA
    CTAAATGGCA ATTTAAAGGT AGACTTTATG GAAACAGAAA ATTCCAGCAG TGTGAAAAAA
    CAAAAAGCGT TAGTAGCAAA AGTGTCTCAG AGGGAAGAGA TGGTTAAAAA ATGTCTTGGA
    GAACTTACAG AAGTCTGCAA ATCTCTGGGG AAAGTTTTTG GTGTCCATTA CTTCAATATT
    TTTAATACCG TCACTCTCAA GAAGCTTGCA GAATCTTTAT CTTCTGATCC TGAGGTTTTG
    CTTCAAATTG ATGGTGTTAC TGAAGACAAA CTGGAAAAAT ATGGTGCGGA AGTGATTTCA
    GTATTACAGA AATACTCTGA ATGGACATCG CCAGCTGAAG ACAGTTCCCC AGGGATAAGC
    CTGTCCAGCA GCAGAGGCCC CGGAAGAAGT GCCGCTGAGG AGCTCGACGA GGAAATACCC
    GTATCTTCCC ACTACTTTGC AAGTAAAACC AGAAATGAAA GGAAGAGGAA AAAGATGCCA
    GCCTCCCAAA GGTCTAAGAG GAGAAAAACT GCTTCCAGTG GTTCCAAGGC AAAGGGGGGG
    TCTGCCACAT GTAGAAAGAT ATCTTCCAAA ACGAAATCCT CCAGCATCAT TGGATCCAGT
    TCAGCCTCAC ATACTTCTCA AGCGACATCA GGAGCCAATA GCAAATTGGG GATTATGGCT
    CCACCGAAGC CTATAAATAG ACCGTTTCTT AAGCCTTCAT ATGCATTCTC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000048.1
    CDS119..4372
    Translation

    Target ORF information:

    RefSeq Version NM_000057.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Bloom syndrome RecQ like helicase (BLM), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000057.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
    CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA
    ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA
    AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC
    ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG
    AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC
    ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA
    TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT
    GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA
    AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA
    ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG
    ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT
    GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT
    CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA
    CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT
    GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT
    ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT
    CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC
    TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT
    AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG
    CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT
    GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG
    GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA
    AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT
    GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT
    GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC
    CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA
    AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC
    TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC
    ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT
    TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA
    GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA
    CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT
    AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG
    CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG
    CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC
    GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT
    AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA
    AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG
    GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC
    AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG
    TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC
    ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTGTTTA GCATGAGCTT TAACAGACAT
    AATCTGAAAT ACTATGTATT ACCGAAAAAG CCTAAAAAGG TGGCATTTGA TTGCCTAGAA
    TGGATCAGAA AGCACCACCC ATATGATTCA GGGATAATTT ACTGCCTCTC CAGGCGAGAA
    TGTGACACCA TGGCTGACAC GTTACAGAGA GATGGGCTCG CTGCTCTTGC TTACCATGCT
    GGCCTCAGTG ATTCTGCCAG AGATGAAGTG CAGCAGAAGT GGATTAATCA GGATGGCTGT
    CAGGTTATCT GTGCTACAAT TGCATTTGGA ATGGGGATTG ACAAACCGGA CGTGCGATTT
    GTGATTCATG CATCTCTCCC TAAATCTGTG GAGGGTTACT ACCAAGAATC TGGCAGAGCT
    GGAAGAGATG GGGAAATATC TCACTGCCTG CTTTTCTATA CCTATCATGA TGTGACCAGA
    CTGAAAAGAC TTATAATGAT GGAAAAAGAT GGAAACCATC ATACAAGAGA AACTCACTTC
    AATAATTTGT ATAGCATGGT ACATTACTGT GAAAATATAA CGGAATGCAG GAGAATACAG
    CTTTTGGCCT ACTTTGGTGA AAATGGATTT AATCCTGATT TTTGTAAGAA ACACCCAGAT
    GTTTCTTGTG ATAATTGCTG TAAAACAAAG GATTATAAAA CAAGAGATGT GACTGACGAT
    GTGAAAAGTA TTGTAAGATT TGTTCAAGAA CATAGTTCAT CACAAGGAAT GAGAAATATA
    AAACATGTAG GTCCTTCTGG AAGATTTACT ATGAATATGC TGGTCGACAT TTTCTTGGGG
    AGTAAGAGTG CAAAAATCCA GTCAGGTATA TTTGGAAAAG GATCTGCTTA TTCACGACAC
    AATGCCGAAA GACTTTTTAA AAAGCTGATA CTTGACAAGA TTTTGGATGA AGACTTATAT
    ATCAATGCCA ATGACCAGGC GATCGCTTAT GTGATGCTCG GAAATAAAGC CCAAACTGTA
    CTAAATGGCA ATTTAAAGGT AGACTTTATG GAAACAGAAA ATTCCAGCAG TGTGAAAAAA
    CAAAAAGCGT TAGTAGCAAA AGTGTCTCAG AGGGAAGAGA TGGTTAAAAA ATGTCTTGGA
    GAACTTACAG AAGTCTGCAA ATCTCTGGGG AAAGTTTTTG GTGTCCATTA CTTCAATATT
    TTTAATACCG TCACTCTCAA GAAGCTTGCA GAATCTTTAT CTTCTGATCC TGAGGTTTTG
    CTTCAAATTG ATGGTGTTAC TGAAGACAAA CTGGAAAAAT ATGGTGCGGA AGTGATTTCA
    GTATTACAGA AATACTCTGA ATGGACATCG CCAGCTGAAG ACAGTTCCCC AGGGATAAGC
    CTGTCCAGCA GCAGAGGCCC CGGAAGAAGT GCCGCTGAGG AGCTCGACGA GGAAATACCC
    GTATCTTCCC ACTACTTTGC AAGTAAAACC AGAAATGAAA GGAAGAGGAA AAAGATGCCA
    GCCTCCCAAA GGTCTAAGAG GAGAAAAACT GCTTCCAGTG GTTCCAAGGC AAAGGGGGGG
    TCTGCCACAT GTAGAAAGAT ATCTTCCAAA ACGAAATCCT CCAGCATCAT TGGATCCAGT
    TCAGCCTCAC ATACTTCTCA AGCGACATCA GGAGCCAATA GCAAATTGGG GATTATGGCT
    CCACCGAAGC CTATAAATAG ACCGTTTCTT AAGCCTTCAT ATGCATTCTC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20947
    Clone ID Related Accession (Same CDS sequence) NM_001287248.2 , NM_001287248.1
    Accession Version NM_001287248.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3129bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product Bloom syndrome protein isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD707743.1, BC062697.1, BC143280.1, AC021422.25, DA122319.1, BC115032.1, DB520927.1 and AC124248.6. On May 31, 2019 this sequence version replaced NM_001287248.1. Transcript Variant: This variant (4) uses an alternate splice site in a 5' exon and uses a downstream translation start compared to variant 1. The resulting protein (isoform 3) is shorter and has a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143280.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGAGCACA TCTGTAAATT AATTGATACT ATTCCTGATG ATAAACTGAA ACTTTTGGAT 
    TGTGGGAACG AACTGCTTCA GCAGCGGAAC ATAAGAAGGA AACTTCTAAC GGAAGTAGAT
    TTTAATAAAA GTGATGCCAG TCTTCTTGGC TCATTGTGGA GATACAGGCC TGATTCACTT
    GATGGCCCTA TGGAGGGTGA TTCCTGCCCT ACAGGGAATT CTATGAAGGA GTTAAATTTT
    TCACACCTTC CCTCAAATTC TGTTTCTCCT GGGGACTGTT TACTGACTAC CACCCTAGGA
    AAGACAGGAT TCTCTGCCAC CAGGAAGAAT CTTTTTGAAA GGCCTTTATT CAATACCCAT
    TTACAGAAGT CCTTTGTAAG TAGCAACTGG GCTGAAACAC CAAGACTAGG AAAAAAAAAT
    GAAAGCTCTT ATTTCCCAGG AAATGTTCTC ACAAGCACTG CTGTGAAAGA TCAGAATAAA
    CATACTGCTT CAATAAATGA CTTAGAAAGA GAAACCCAAC CTTCCTATGA TATTGATAAT
    TTTGACATAG ATGACTTTGA TGATGATGAT GACTGGGAAG ACATAATGCA TAATTTAGCA
    GCCAGCAAAT CTTCCACAGC TGCCTATCAA CCCATCAAGG AAGGTCGGCC AATTAAATCA
    GTATCAGAAA GACTTTCCTC AGCCAAGACA GACTGTCTTC CAGTGTCATC TACTGCTCAA
    AATATAAACT TCTCAGAGTC AATTCAGAAT TATACTGACA AGTCAGCACA AAATTTAGCA
    TCCAGAAATC TGAAACATGA GCGTTTCCAA AGTCTTAGTT TTCCTCATAC AAAGGAAATG
    ATGAAGATTT TTCATAAAAA ATTTGGCCTG CATAATTTTA GAACTAATCA GCTAGAGGCG
    ATCAATGCTG CACTGCTTGG TGAAGACTGT TTTATCCTGA TGCCGACTGG AGGTGGTAAG
    AGTTTGTGTT ACCAGCTCCC TGCCTGTGTT TCTCCTGGGG TCACTGTTGT CATTTCTCCC
    TTGAGATCAC TTATCGTAGA TCAAGTCCAA AAGCTGACTT CCTTGGATAT TCCAGCTACA
    TATCTGACAG GTGATAAGAC TGACTCAGAA GCTACAAATA TTTACCTCCA GTTATCAAAA
    AAAGACCCAA TCATAAAACT TCTATATGTC ACTCCAGAAA AGATCTGTGC AAGTAACAGA
    CTCATTTCTA CTCTGGAGAA TCTCTATGAG AGGAAGCTCT TGGCACGTTT TGTTATTGAT
    GAAGCACATT GTGTCAGTCA GTGGGGACAT GATTTTCGTC AAGATTACAA AAGAATGAAT
    ATGCTTCGCC AGAAGTTTCC TTCTGTTCCG GTGATGGCTC TTACGGCCAC AGCTAATCCC
    AGGGTACAGA AGGACATCCT GACTCAGCTG AAGATTCTCA GACCTCAGGT GTTTAGCATG
    AGCTTTAACA GACATAATCT GAAATACTAT GTATTACCGA AAAAGCCTAA AAAGGTGGCA
    TTTGATTGCC TAGAATGGAT CAGAAAGCAC CACCCATATG ATTCAGGGAT AATTTACTGC
    CTCTCCAGGC GAGAATGTGA CACCATGGCT GACACGTTAC AGAGAGATGG GCTCGCTGCT
    CTTGCTTACC ATGCTGGCCT CAGTGATTCT GCCAGAGATG AAGTGCAGCA GAAGTGGATT
    AATCAGGATG GCTGTCAGGT TATCTGTGCT ACAATTGCAT TTGGAATGGG GATTGACAAA
    CCGGACGTGC GATTTGTGAT TCATGCATCT CTCCCTAAAT CTGTGGAGGG TTACTACCAA
    GAATCTGGCA GAGCTGGAAG AGATGGGGAA ATATCTCACT GCCTGCTTTT CTATACCTAT
    CATGATGTGA CCAGACTGAA AAGACTTATA ATGATGGAAA AAGATGGAAA CCATCATACA
    AGAGAAACTC ACTTCAATAA TTTGTATAGC ATGGTACATT ACTGTGAAAA TATAACGGAA
    TGCAGGAGAA TACAGCTTTT GGCCTACTTT GGTGAAAATG GATTTAATCC TGATTTTTGT
    AAGAAACACC CAGATGTTTC TTGTGATAAT TGCTGTAAAA CAAAGGATTA TAAAACAAGA
    GATGTGACTG ACGATGTGAA AAGTATTGTA AGATTTGTTC AAGAACATAG TTCATCACAA
    GGAATGAGAA ATATAAAACA TGTAGGTCCT TCTGGAAGAT TTACTATGAA TATGCTGGTC
    GACATTTTCT TGGGGAGTAA GAGTGCAAAA ATCCAGTCAG GTATATTTGG AAAAGGATCT
    GCTTATTCAC GACACAATGC CGAAAGACTT TTTAAAAAGC TGATACTTGA CAAGATTTTG
    GATGAAGACT TATATATCAA TGCCAATGAC CAGGCGATCG CTTATGTGAT GCTCGGAAAT
    AAAGCCCAAA CTGTACTAAA TGGCAATTTA AAGGTAGACT TTATGGAAAC AGAAAATTCC
    AGCAGTGTGA AAAAACAAAA AGCGTTAGTA GCAAAAGTGT CTCAGAGGGA AGAGATGGTT
    AAAAAATGTC TTGGAGAACT TACAGAAGTC TGCAAATCTC TGGGGAAAGT TTTTGGTGTC
    CATTACTTCA ATATTTTTAA TACCGTCACT CTCAAGAAGC TTGCAGAATC TTTATCTTCT
    GATCCTGAGG TTTTGCTTCA AATTGATGGT GTTACTGAAG ACAAACTGGA AAAATATGGT
    GCGGAAGTGA TTTCAGTATT ACAGAAATAC TCTGAATGGA CATCGCCAGC TGAAGACAGT
    TCCCCAGGGA TAAGCCTGTC CAGCAGCAGA GGCCCCGGAA GAAGTGCCGC TGAGGAGCTC
    GACGAGGAAA TACCCGTATC TTCCCACTAC TTTGCAAGTA AAACCAGAAA TGAAAGGAAG
    AGGAAAAAGA TGCCAGCCTC CCAAAGGTCT AAGAGGAGAA AAACTGCTTC CAGTGGTTCC
    AAGGCAAAGG GGGGGTCTGC CACATGTAGA AAGATATCTT CCAAAACGAA ATCCTCCAGC
    ATCATTGGAT CCAGTTCAGC CTCACATACT TCTCAAGCGA CATCAGGAGC CAATAGCAAA
    TTGGGGATTA TGGCTCCACC GAAGCCTATA AATAGACCGT TTCTTAAGCC TTCATATGCA
    TTCTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001274177.1
    CDS1391..4519
    Translation

    Target ORF information:

    RefSeq Version NM_001287248.2
    Organism Homo sapiens(human)
    Definition Homo sapiens BLM RecQ like helicase (BLM), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001287248.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGAGCACA TCTGTAAATT AATTGATACT ATTCCTGATG ATAAACTGAA ACTTTTGGAT 
    TGTGGGAACG AACTGCTTCA GCAGCGGAAC ATAAGAAGGA AACTTCTAAC GGAAGTAGAT
    TTTAATAAAA GTGATGCCAG TCTTCTTGGC TCATTGTGGA GATACAGGCC TGATTCACTT
    GATGGCCCTA TGGAGGGTGA TTCCTGCCCT ACAGGGAATT CTATGAAGGA GTTAAATTTT
    TCACACCTTC CCTCAAATTC TGTTTCTCCT GGGGACTGTT TACTGACTAC CACCCTAGGA
    AAGACAGGAT TCTCTGCCAC CAGGAAGAAT CTTTTTGAAA GGCCTTTATT CAATACCCAT
    TTACAGAAGT CCTTTGTAAG TAGCAACTGG GCTGAAACAC CAAGACTAGG AAAAAAAAAT
    GAAAGCTCTT ATTTCCCAGG AAATGTTCTC ACAAGCACTG CTGTGAAAGA TCAGAATAAA
    CATACTGCTT CAATAAATGA CTTAGAAAGA GAAACCCAAC CTTCCTATGA TATTGATAAT
    TTTGACATAG ATGACTTTGA TGATGATGAT GACTGGGAAG ACATAATGCA TAATTTAGCA
    GCCAGCAAAT CTTCCACAGC TGCCTATCAA CCCATCAAGG AAGGTCGGCC AATTAAATCA
    GTATCAGAAA GACTTTCCTC AGCCAAGACA GACTGTCTTC CAGTGTCATC TACTGCTCAA
    AATATAAACT TCTCAGAGTC AATTCAGAAT TATACTGACA AGTCAGCACA AAATTTAGCA
    TCCAGAAATC TGAAACATGA GCGTTTCCAA AGTCTTAGTT TTCCTCATAC AAAGGAAATG
    ATGAAGATTT TTCATAAAAA ATTTGGCCTG CATAATTTTA GAACTAATCA GCTAGAGGCG
    ATCAATGCTG CACTGCTTGG TGAAGACTGT TTTATCCTGA TGCCGACTGG AGGTGGTAAG
    AGTTTGTGTT ACCAGCTCCC TGCCTGTGTT TCTCCTGGGG TCACTGTTGT CATTTCTCCC
    TTGAGATCAC TTATCGTAGA TCAAGTCCAA AAGCTGACTT CCTTGGATAT TCCAGCTACA
    TATCTGACAG GTGATAAGAC TGACTCAGAA GCTACAAATA TTTACCTCCA GTTATCAAAA
    AAAGACCCAA TCATAAAACT TCTATATGTC ACTCCAGAAA AGATCTGTGC AAGTAACAGA
    CTCATTTCTA CTCTGGAGAA TCTCTATGAG AGGAAGCTCT TGGCACGTTT TGTTATTGAT
    GAAGCACATT GTGTCAGTCA GTGGGGACAT GATTTTCGTC AAGATTACAA AAGAATGAAT
    ATGCTTCGCC AGAAGTTTCC TTCTGTTCCG GTGATGGCTC TTACGGCCAC AGCTAATCCC
    AGGGTACAGA AGGACATCCT GACTCAGCTG AAGATTCTCA GACCTCAGGT GTTTAGCATG
    AGCTTTAACA GACATAATCT GAAATACTAT GTATTACCGA AAAAGCCTAA AAAGGTGGCA
    TTTGATTGCC TAGAATGGAT CAGAAAGCAC CACCCATATG ATTCAGGGAT AATTTACTGC
    CTCTCCAGGC GAGAATGTGA CACCATGGCT GACACGTTAC AGAGAGATGG GCTCGCTGCT
    CTTGCTTACC ATGCTGGCCT CAGTGATTCT GCCAGAGATG AAGTGCAGCA GAAGTGGATT
    AATCAGGATG GCTGTCAGGT TATCTGTGCT ACAATTGCAT TTGGAATGGG GATTGACAAA
    CCGGACGTGC GATTTGTGAT TCATGCATCT CTCCCTAAAT CTGTGGAGGG TTACTACCAA
    GAATCTGGCA GAGCTGGAAG AGATGGGGAA ATATCTCACT GCCTGCTTTT CTATACCTAT
    CATGATGTGA CCAGACTGAA AAGACTTATA ATGATGGAAA AAGATGGAAA CCATCATACA
    AGAGAAACTC ACTTCAATAA TTTGTATAGC ATGGTACATT ACTGTGAAAA TATAACGGAA
    TGCAGGAGAA TACAGCTTTT GGCCTACTTT GGTGAAAATG GATTTAATCC TGATTTTTGT
    AAGAAACACC CAGATGTTTC TTGTGATAAT TGCTGTAAAA CAAAGGATTA TAAAACAAGA
    GATGTGACTG ACGATGTGAA AAGTATTGTA AGATTTGTTC AAGAACATAG TTCATCACAA
    GGAATGAGAA ATATAAAACA TGTAGGTCCT TCTGGAAGAT TTACTATGAA TATGCTGGTC
    GACATTTTCT TGGGGAGTAA GAGTGCAAAA ATCCAGTCAG GTATATTTGG AAAAGGATCT
    GCTTATTCAC GACACAATGC CGAAAGACTT TTTAAAAAGC TGATACTTGA CAAGATTTTG
    GATGAAGACT TATATATCAA TGCCAATGAC CAGGCGATCG CTTATGTGAT GCTCGGAAAT
    AAAGCCCAAA CTGTACTAAA TGGCAATTTA AAGGTAGACT TTATGGAAAC AGAAAATTCC
    AGCAGTGTGA AAAAACAAAA AGCGTTAGTA GCAAAAGTGT CTCAGAGGGA AGAGATGGTT
    AAAAAATGTC TTGGAGAACT TACAGAAGTC TGCAAATCTC TGGGGAAAGT TTTTGGTGTC
    CATTACTTCA ATATTTTTAA TACCGTCACT CTCAAGAAGC TTGCAGAATC TTTATCTTCT
    GATCCTGAGG TTTTGCTTCA AATTGATGGT GTTACTGAAG ACAAACTGGA AAAATATGGT
    GCGGAAGTGA TTTCAGTATT ACAGAAATAC TCTGAATGGA CATCGCCAGC TGAAGACAGT
    TCCCCAGGGA TAAGCCTGTC CAGCAGCAGA GGCCCCGGAA GAAGTGCCGC TGAGGAGCTC
    GACGAGGAAA TACCCGTATC TTCCCACTAC TTTGCAAGTA AAACCAGAAA TGAAAGGAAG
    AGGAAAAAGA TGCCAGCCTC CCAAAGGTCT AAGAGGAGAA AAACTGCTTC CAGTGGTTCC
    AAGGCAAAGG GGGGGTCTGC CACATGTAGA AAGATATCTT CCAAAACGAA ATCCTCCAGC
    ATCATTGGAT CCAGTTCAGC CTCACATACT TCTCAAGCGA CATCAGGAGC CAATAGCAAA
    TTGGGGATTA TGGCTCCACC GAAGCCTATA AATAGACCGT TTCTTAAGCC TTCATATGCA
    TTCTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20947
    Clone ID Related Accession (Same CDS sequence) NM_001287248.2 , NM_001287248.1
    Accession Version NM_001287248.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3129bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product Bloom syndrome protein isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD707743.1, BC062697.1, BC143280.1, AC021422.25, DA122319.1, BC115032.1, DB520927.1 and AC124248.6. Transcript Variant: This variant (4) uses an alternate splice site in a 5' exon and uses a downstream translation start compared to variant 1. The resulting protein (isoform 3) is shorter and has a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143280.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGAGCACA TCTGTAAATT AATTGATACT ATTCCTGATG ATAAACTGAA ACTTTTGGAT 
    TGTGGGAACG AACTGCTTCA GCAGCGGAAC ATAAGAAGGA AACTTCTAAC GGAAGTAGAT
    TTTAATAAAA GTGATGCCAG TCTTCTTGGC TCATTGTGGA GATACAGGCC TGATTCACTT
    GATGGCCCTA TGGAGGGTGA TTCCTGCCCT ACAGGGAATT CTATGAAGGA GTTAAATTTT
    TCACACCTTC CCTCAAATTC TGTTTCTCCT GGGGACTGTT TACTGACTAC CACCCTAGGA
    AAGACAGGAT TCTCTGCCAC CAGGAAGAAT CTTTTTGAAA GGCCTTTATT CAATACCCAT
    TTACAGAAGT CCTTTGTAAG TAGCAACTGG GCTGAAACAC CAAGACTAGG AAAAAAAAAT
    GAAAGCTCTT ATTTCCCAGG AAATGTTCTC ACAAGCACTG CTGTGAAAGA TCAGAATAAA
    CATACTGCTT CAATAAATGA CTTAGAAAGA GAAACCCAAC CTTCCTATGA TATTGATAAT
    TTTGACATAG ATGACTTTGA TGATGATGAT GACTGGGAAG ACATAATGCA TAATTTAGCA
    GCCAGCAAAT CTTCCACAGC TGCCTATCAA CCCATCAAGG AAGGTCGGCC AATTAAATCA
    GTATCAGAAA GACTTTCCTC AGCCAAGACA GACTGTCTTC CAGTGTCATC TACTGCTCAA
    AATATAAACT TCTCAGAGTC AATTCAGAAT TATACTGACA AGTCAGCACA AAATTTAGCA
    TCCAGAAATC TGAAACATGA GCGTTTCCAA AGTCTTAGTT TTCCTCATAC AAAGGAAATG
    ATGAAGATTT TTCATAAAAA ATTTGGCCTG CATAATTTTA GAACTAATCA GCTAGAGGCG
    ATCAATGCTG CACTGCTTGG TGAAGACTGT TTTATCCTGA TGCCGACTGG AGGTGGTAAG
    AGTTTGTGTT ACCAGCTCCC TGCCTGTGTT TCTCCTGGGG TCACTGTTGT CATTTCTCCC
    TTGAGATCAC TTATCGTAGA TCAAGTCCAA AAGCTGACTT CCTTGGATAT TCCAGCTACA
    TATCTGACAG GTGATAAGAC TGACTCAGAA GCTACAAATA TTTACCTCCA GTTATCAAAA
    AAAGACCCAA TCATAAAACT TCTATATGTC ACTCCAGAAA AGATCTGTGC AAGTAACAGA
    CTCATTTCTA CTCTGGAGAA TCTCTATGAG AGGAAGCTCT TGGCACGTTT TGTTATTGAT
    GAAGCACATT GTGTCAGTCA GTGGGGACAT GATTTTCGTC AAGATTACAA AAGAATGAAT
    ATGCTTCGCC AGAAGTTTCC TTCTGTTCCG GTGATGGCTC TTACGGCCAC AGCTAATCCC
    AGGGTACAGA AGGACATCCT GACTCAGCTG AAGATTCTCA GACCTCAGGT GTTTAGCATG
    AGCTTTAACA GACATAATCT GAAATACTAT GTATTACCGA AAAAGCCTAA AAAGGTGGCA
    TTTGATTGCC TAGAATGGAT CAGAAAGCAC CACCCATATG ATTCAGGGAT AATTTACTGC
    CTCTCCAGGC GAGAATGTGA CACCATGGCT GACACGTTAC AGAGAGATGG GCTCGCTGCT
    CTTGCTTACC ATGCTGGCCT CAGTGATTCT GCCAGAGATG AAGTGCAGCA GAAGTGGATT
    AATCAGGATG GCTGTCAGGT TATCTGTGCT ACAATTGCAT TTGGAATGGG GATTGACAAA
    CCGGACGTGC GATTTGTGAT TCATGCATCT CTCCCTAAAT CTGTGGAGGG TTACTACCAA
    GAATCTGGCA GAGCTGGAAG AGATGGGGAA ATATCTCACT GCCTGCTTTT CTATACCTAT
    CATGATGTGA CCAGACTGAA AAGACTTATA ATGATGGAAA AAGATGGAAA CCATCATACA
    AGAGAAACTC ACTTCAATAA TTTGTATAGC ATGGTACATT ACTGTGAAAA TATAACGGAA
    TGCAGGAGAA TACAGCTTTT GGCCTACTTT GGTGAAAATG GATTTAATCC TGATTTTTGT
    AAGAAACACC CAGATGTTTC TTGTGATAAT TGCTGTAAAA CAAAGGATTA TAAAACAAGA
    GATGTGACTG ACGATGTGAA AAGTATTGTA AGATTTGTTC AAGAACATAG TTCATCACAA
    GGAATGAGAA ATATAAAACA TGTAGGTCCT TCTGGAAGAT TTACTATGAA TATGCTGGTC
    GACATTTTCT TGGGGAGTAA GAGTGCAAAA ATCCAGTCAG GTATATTTGG AAAAGGATCT
    GCTTATTCAC GACACAATGC CGAAAGACTT TTTAAAAAGC TGATACTTGA CAAGATTTTG
    GATGAAGACT TATATATCAA TGCCAATGAC CAGGCGATCG CTTATGTGAT GCTCGGAAAT
    AAAGCCCAAA CTGTACTAAA TGGCAATTTA AAGGTAGACT TTATGGAAAC AGAAAATTCC
    AGCAGTGTGA AAAAACAAAA AGCGTTAGTA GCAAAAGTGT CTCAGAGGGA AGAGATGGTT
    AAAAAATGTC TTGGAGAACT TACAGAAGTC TGCAAATCTC TGGGGAAAGT TTTTGGTGTC
    CATTACTTCA ATATTTTTAA TACCGTCACT CTCAAGAAGC TTGCAGAATC TTTATCTTCT
    GATCCTGAGG TTTTGCTTCA AATTGATGGT GTTACTGAAG ACAAACTGGA AAAATATGGT
    GCGGAAGTGA TTTCAGTATT ACAGAAATAC TCTGAATGGA CATCGCCAGC TGAAGACAGT
    TCCCCAGGGA TAAGCCTGTC CAGCAGCAGA GGCCCCGGAA GAAGTGCCGC TGAGGAGCTC
    GACGAGGAAA TACCCGTATC TTCCCACTAC TTTGCAAGTA AAACCAGAAA TGAAAGGAAG
    AGGAAAAAGA TGCCAGCCTC CCAAAGGTCT AAGAGGAGAA AAACTGCTTC CAGTGGTTCC
    AAGGCAAAGG GGGGGTCTGC CACATGTAGA AAGATATCTT CCAAAACGAA ATCCTCCAGC
    ATCATTGGAT CCAGTTCAGC CTCACATACT TCTCAAGCGA CATCAGGAGC CAATAGCAAA
    TTGGGGATTA TGGCTCCACC GAAGCCTATA AATAGACCGT TTCTTAAGCC TTCATATGCA
    TTCTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001274177.1
    CDS1410..4538
    Translation

    Target ORF information:

    RefSeq Version NM_001287248.1
    Organism Homo sapiens(human)
    Definition Homo sapiens BLM RecQ like helicase (BLM), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001287248.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGAGCACA TCTGTAAATT AATTGATACT ATTCCTGATG ATAAACTGAA ACTTTTGGAT 
    TGTGGGAACG AACTGCTTCA GCAGCGGAAC ATAAGAAGGA AACTTCTAAC GGAAGTAGAT
    TTTAATAAAA GTGATGCCAG TCTTCTTGGC TCATTGTGGA GATACAGGCC TGATTCACTT
    GATGGCCCTA TGGAGGGTGA TTCCTGCCCT ACAGGGAATT CTATGAAGGA GTTAAATTTT
    TCACACCTTC CCTCAAATTC TGTTTCTCCT GGGGACTGTT TACTGACTAC CACCCTAGGA
    AAGACAGGAT TCTCTGCCAC CAGGAAGAAT CTTTTTGAAA GGCCTTTATT CAATACCCAT
    TTACAGAAGT CCTTTGTAAG TAGCAACTGG GCTGAAACAC CAAGACTAGG AAAAAAAAAT
    GAAAGCTCTT ATTTCCCAGG AAATGTTCTC ACAAGCACTG CTGTGAAAGA TCAGAATAAA
    CATACTGCTT CAATAAATGA CTTAGAAAGA GAAACCCAAC CTTCCTATGA TATTGATAAT
    TTTGACATAG ATGACTTTGA TGATGATGAT GACTGGGAAG ACATAATGCA TAATTTAGCA
    GCCAGCAAAT CTTCCACAGC TGCCTATCAA CCCATCAAGG AAGGTCGGCC AATTAAATCA
    GTATCAGAAA GACTTTCCTC AGCCAAGACA GACTGTCTTC CAGTGTCATC TACTGCTCAA
    AATATAAACT TCTCAGAGTC AATTCAGAAT TATACTGACA AGTCAGCACA AAATTTAGCA
    TCCAGAAATC TGAAACATGA GCGTTTCCAA AGTCTTAGTT TTCCTCATAC AAAGGAAATG
    ATGAAGATTT TTCATAAAAA ATTTGGCCTG CATAATTTTA GAACTAATCA GCTAGAGGCG
    ATCAATGCTG CACTGCTTGG TGAAGACTGT TTTATCCTGA TGCCGACTGG AGGTGGTAAG
    AGTTTGTGTT ACCAGCTCCC TGCCTGTGTT TCTCCTGGGG TCACTGTTGT CATTTCTCCC
    TTGAGATCAC TTATCGTAGA TCAAGTCCAA AAGCTGACTT CCTTGGATAT TCCAGCTACA
    TATCTGACAG GTGATAAGAC TGACTCAGAA GCTACAAATA TTTACCTCCA GTTATCAAAA
    AAAGACCCAA TCATAAAACT TCTATATGTC ACTCCAGAAA AGATCTGTGC AAGTAACAGA
    CTCATTTCTA CTCTGGAGAA TCTCTATGAG AGGAAGCTCT TGGCACGTTT TGTTATTGAT
    GAAGCACATT GTGTCAGTCA GTGGGGACAT GATTTTCGTC AAGATTACAA AAGAATGAAT
    ATGCTTCGCC AGAAGTTTCC TTCTGTTCCG GTGATGGCTC TTACGGCCAC AGCTAATCCC
    AGGGTACAGA AGGACATCCT GACTCAGCTG AAGATTCTCA GACCTCAGGT GTTTAGCATG
    AGCTTTAACA GACATAATCT GAAATACTAT GTATTACCGA AAAAGCCTAA AAAGGTGGCA
    TTTGATTGCC TAGAATGGAT CAGAAAGCAC CACCCATATG ATTCAGGGAT AATTTACTGC
    CTCTCCAGGC GAGAATGTGA CACCATGGCT GACACGTTAC AGAGAGATGG GCTCGCTGCT
    CTTGCTTACC ATGCTGGCCT CAGTGATTCT GCCAGAGATG AAGTGCAGCA GAAGTGGATT
    AATCAGGATG GCTGTCAGGT TATCTGTGCT ACAATTGCAT TTGGAATGGG GATTGACAAA
    CCGGACGTGC GATTTGTGAT TCATGCATCT CTCCCTAAAT CTGTGGAGGG TTACTACCAA
    GAATCTGGCA GAGCTGGAAG AGATGGGGAA ATATCTCACT GCCTGCTTTT CTATACCTAT
    CATGATGTGA CCAGACTGAA AAGACTTATA ATGATGGAAA AAGATGGAAA CCATCATACA
    AGAGAAACTC ACTTCAATAA TTTGTATAGC ATGGTACATT ACTGTGAAAA TATAACGGAA
    TGCAGGAGAA TACAGCTTTT GGCCTACTTT GGTGAAAATG GATTTAATCC TGATTTTTGT
    AAGAAACACC CAGATGTTTC TTGTGATAAT TGCTGTAAAA CAAAGGATTA TAAAACAAGA
    GATGTGACTG ACGATGTGAA AAGTATTGTA AGATTTGTTC AAGAACATAG TTCATCACAA
    GGAATGAGAA ATATAAAACA TGTAGGTCCT TCTGGAAGAT TTACTATGAA TATGCTGGTC
    GACATTTTCT TGGGGAGTAA GAGTGCAAAA ATCCAGTCAG GTATATTTGG AAAAGGATCT
    GCTTATTCAC GACACAATGC CGAAAGACTT TTTAAAAAGC TGATACTTGA CAAGATTTTG
    GATGAAGACT TATATATCAA TGCCAATGAC CAGGCGATCG CTTATGTGAT GCTCGGAAAT
    AAAGCCCAAA CTGTACTAAA TGGCAATTTA AAGGTAGACT TTATGGAAAC AGAAAATTCC
    AGCAGTGTGA AAAAACAAAA AGCGTTAGTA GCAAAAGTGT CTCAGAGGGA AGAGATGGTT
    AAAAAATGTC TTGGAGAACT TACAGAAGTC TGCAAATCTC TGGGGAAAGT TTTTGGTGTC
    CATTACTTCA ATATTTTTAA TACCGTCACT CTCAAGAAGC TTGCAGAATC TTTATCTTCT
    GATCCTGAGG TTTTGCTTCA AATTGATGGT GTTACTGAAG ACAAACTGGA AAAATATGGT
    GCGGAAGTGA TTTCAGTATT ACAGAAATAC TCTGAATGGA CATCGCCAGC TGAAGACAGT
    TCCCCAGGGA TAAGCCTGTC CAGCAGCAGA GGCCCCGGAA GAAGTGCCGC TGAGGAGCTC
    GACGAGGAAA TACCCGTATC TTCCCACTAC TTTGCAAGTA AAACCAGAAA TGAAAGGAAG
    AGGAAAAAGA TGCCAGCCTC CCAAAGGTCT AAGAGGAGAA AAACTGCTTC CAGTGGTTCC
    AAGGCAAAGG GGGGGTCTGC CACATGTAGA AAGATATCTT CCAAAACGAA ATCCTCCAGC
    ATCATTGGAT CCAGTTCAGC CTCACATACT TCTCAAGCGA CATCAGGAGC CAATAGCAAA
    TTGGGGATTA TGGCTCCACC GAAGCCTATA AATAGACCGT TTCTTAAGCC TTCATATGCA
    TTCTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20391
    Clone ID Related Accession (Same CDS sequence) NM_001287247.1 , NM_001287247.2
    Accession Version NM_001287247.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3861bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product Bloom syndrome protein isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD707743.1, BC062697.1, BC115028.1, BC101567.1, DB520927.1 and AC124248.6. Transcript Variant: This variant (3) lacks two alternate in-frame exons in the 3' coding region compared to variant 1. The resulting protein (isoform 2) is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC115028.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA2150385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
    CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA
    ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA
    AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC
    ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG
    AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC
    ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA
    TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT
    GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA
    AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA
    ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG
    ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT
    GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT
    CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA
    CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT
    GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT
    ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT
    CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC
    TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT
    AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG
    CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT
    GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG
    GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA
    AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT
    GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT
    GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC
    CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA
    AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC
    TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC
    ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT
    TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA
    GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA
    CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT
    AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG
    CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG
    CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC
    GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT
    AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA
    AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG
    GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC
    AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG
    TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC
    ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTGTTTA GCATGAGCTT TAACAGACAT
    AATCTGAAAT ACTATGTATT ACCGAAAAAG CCTAAAAAGG TGGCATTTGA TTGCCTAGAA
    TGGATCAGAA AGCACCACCC ATATGATTCA GGGATAATTT ACTGCCTCTC CAGGCGAGAA
    TGTGACACCA TGGCTGACAC GTTACAGAGA GATGGGCTCG CTGCTCTTGC TTACCATGCT
    GGCCTCAGTG ATTCTGCCAG AGATGAAGTG CAGCAGAAGT GGATTAATCA GGATGGCTGT
    CAGGTTATCT GTGCTACAAT TGCATTTGGA ATGGGGATTG ACAAACCGGA CGTGCGATTT
    GTGATTCATG CATCTCTCCC TAAATCTGTG GAGGGTTACT ACCAAGAATC TGGCAGAGCT
    GGAAGAGATG GGGAAATATC TCACTGCCTG CTTTTCTATA CCTATCATGA TGTGACCAGA
    CTGAAAAGAC TTATAATGAT GGAAAAAGAT GGAAACCATC ATACAAGAGA AACTCACTTC
    AATAATTTGT ATAGCATGGT ACATTACTGT GAAAATATAA CGGAATGCAG GAGAATACAG
    CTTTTGGCCT ACTTTGGTGA AAATGGATTT AATCCTGATT TTTGTAAGAA ACACCCAGAT
    GTTTCTTGTG ATAATTGCTG TAAAACAAAG GATTATAAAA CAAGAGATGT GACTGACGAT
    GTGAAAAGTA TTGTAAGATT TGTTCAAGAA CATAGTTCAT CACAAGGAAT GAGAAATATA
    AAACATGTAG GTCCTTCTGG AAGATTTACT ATGAATATGC TGGTCGACAT TTTCTTGGAA
    TCTTTATCTT CTGATCCTGA GGTTTTGCTT CAAATTGATG GTGTTACTGA AGACAAACTG
    GAAAAATATG GTGCGGAAGT GATTTCAGTA TTACAGAAAT ACTCTGAATG GACATCGCCA
    GCTGAAGACA GTTCCCCAGG GATAAGCCTG TCCAGCAGCA GAGGCCCCGG AAGAAGTGCC
    GCTGAGGAGC TCGACGAGGA AATACCCGTA TCTTCCCACT ACTTTGCAAG TAAAACCAGA
    AATGAAAGGA AGAGGAAAAA GATGCCAGCC TCCCAAAGGT CTAAGAGGAG AAAAACTGCT
    TCCAGTGGTT CCAAGGCAAA GGGGGGGTCT GCCACATGTA GAAAGATATC TTCCAAAACG
    AAATCCTCCA GCATCATTGG ATCCAGTTCA GCCTCACATA CTTCTCAAGC GACATCAGGA
    GCCAATAGCA AATTGGGGAT TATGGCTCCA CCGAAGCCTA TAAATAGACC GTTTCTTAAG
    CCTTCATATG CATTCTCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001274176.1
    CDS119..3979
    Translation

    Target ORF information:

    RefSeq Version NM_001287247.1
    Organism Homo sapiens(human)
    Definition Homo sapiens BLM RecQ like helicase (BLM), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001287247.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
    CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA
    ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA
    AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC
    ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG
    AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC
    ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA
    TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT
    GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA
    AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA
    ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG
    ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT
    GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT
    CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA
    CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT
    GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT
    ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT
    CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC
    TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT
    AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG
    CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT
    GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG
    GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA
    AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT
    GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT
    GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC
    CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA
    AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC
    TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC
    ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT
    TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA
    GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA
    CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT
    AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG
    CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG
    CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC
    GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT
    AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA
    AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG
    GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC
    AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG
    TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC
    ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTGTTTA GCATGAGCTT TAACAGACAT
    AATCTGAAAT ACTATGTATT ACCGAAAAAG CCTAAAAAGG TGGCATTTGA TTGCCTAGAA
    TGGATCAGAA AGCACCACCC ATATGATTCA GGGATAATTT ACTGCCTCTC CAGGCGAGAA
    TGTGACACCA TGGCTGACAC GTTACAGAGA GATGGGCTCG CTGCTCTTGC TTACCATGCT
    GGCCTCAGTG ATTCTGCCAG AGATGAAGTG CAGCAGAAGT GGATTAATCA GGATGGCTGT
    CAGGTTATCT GTGCTACAAT TGCATTTGGA ATGGGGATTG ACAAACCGGA CGTGCGATTT
    GTGATTCATG CATCTCTCCC TAAATCTGTG GAGGGTTACT ACCAAGAATC TGGCAGAGCT
    GGAAGAGATG GGGAAATATC TCACTGCCTG CTTTTCTATA CCTATCATGA TGTGACCAGA
    CTGAAAAGAC TTATAATGAT GGAAAAAGAT GGAAACCATC ATACAAGAGA AACTCACTTC
    AATAATTTGT ATAGCATGGT ACATTACTGT GAAAATATAA CGGAATGCAG GAGAATACAG
    CTTTTGGCCT ACTTTGGTGA AAATGGATTT AATCCTGATT TTTGTAAGAA ACACCCAGAT
    GTTTCTTGTG ATAATTGCTG TAAAACAAAG GATTATAAAA CAAGAGATGT GACTGACGAT
    GTGAAAAGTA TTGTAAGATT TGTTCAAGAA CATAGTTCAT CACAAGGAAT GAGAAATATA
    AAACATGTAG GTCCTTCTGG AAGATTTACT ATGAATATGC TGGTCGACAT TTTCTTGGAA
    TCTTTATCTT CTGATCCTGA GGTTTTGCTT CAAATTGATG GTGTTACTGA AGACAAACTG
    GAAAAATATG GTGCGGAAGT GATTTCAGTA TTACAGAAAT ACTCTGAATG GACATCGCCA
    GCTGAAGACA GTTCCCCAGG GATAAGCCTG TCCAGCAGCA GAGGCCCCGG AAGAAGTGCC
    GCTGAGGAGC TCGACGAGGA AATACCCGTA TCTTCCCACT ACTTTGCAAG TAAAACCAGA
    AATGAAAGGA AGAGGAAAAA GATGCCAGCC TCCCAAAGGT CTAAGAGGAG AAAAACTGCT
    TCCAGTGGTT CCAAGGCAAA GGGGGGGTCT GCCACATGTA GAAAGATATC TTCCAAAACG
    AAATCCTCCA GCATCATTGG ATCCAGTTCA GCCTCACATA CTTCTCAAGC GACATCAGGA
    GCCAATAGCA AATTGGGGAT TATGGCTCCA CCGAAGCCTA TAAATAGACC GTTTCTTAAG
    CCTTCATATG CATTCTCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20391
    Clone ID Related Accession (Same CDS sequence) NM_001287247.1 , NM_001287247.2
    Accession Version NM_001287247.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3861bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product Bloom syndrome protein isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD707743.1, BC062697.1, BC115028.1, BC101567.1, DB520927.1 and AC124248.6. On May 31, 2019 this sequence version replaced NM_001287247.1. Transcript Variant: This variant (3) lacks two alternate in-frame exons in the 3' coding region compared to variant 1. The resulting protein (isoform 2) is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC115028.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA2150385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
    CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA
    ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA
    AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC
    ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG
    AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC
    ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA
    TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT
    GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA
    AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA
    ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG
    ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT
    GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT
    CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA
    CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT
    GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT
    ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT
    CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC
    TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT
    AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG
    CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT
    GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG
    GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA
    AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT
    GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT
    GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC
    CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA
    AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC
    TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC
    ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT
    TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA
    GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA
    CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT
    AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG
    CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG
    CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC
    GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT
    AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA
    AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG
    GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC
    AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG
    TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC
    ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTGTTTA GCATGAGCTT TAACAGACAT
    AATCTGAAAT ACTATGTATT ACCGAAAAAG CCTAAAAAGG TGGCATTTGA TTGCCTAGAA
    TGGATCAGAA AGCACCACCC ATATGATTCA GGGATAATTT ACTGCCTCTC CAGGCGAGAA
    TGTGACACCA TGGCTGACAC GTTACAGAGA GATGGGCTCG CTGCTCTTGC TTACCATGCT
    GGCCTCAGTG ATTCTGCCAG AGATGAAGTG CAGCAGAAGT GGATTAATCA GGATGGCTGT
    CAGGTTATCT GTGCTACAAT TGCATTTGGA ATGGGGATTG ACAAACCGGA CGTGCGATTT
    GTGATTCATG CATCTCTCCC TAAATCTGTG GAGGGTTACT ACCAAGAATC TGGCAGAGCT
    GGAAGAGATG GGGAAATATC TCACTGCCTG CTTTTCTATA CCTATCATGA TGTGACCAGA
    CTGAAAAGAC TTATAATGAT GGAAAAAGAT GGAAACCATC ATACAAGAGA AACTCACTTC
    AATAATTTGT ATAGCATGGT ACATTACTGT GAAAATATAA CGGAATGCAG GAGAATACAG
    CTTTTGGCCT ACTTTGGTGA AAATGGATTT AATCCTGATT TTTGTAAGAA ACACCCAGAT
    GTTTCTTGTG ATAATTGCTG TAAAACAAAG GATTATAAAA CAAGAGATGT GACTGACGAT
    GTGAAAAGTA TTGTAAGATT TGTTCAAGAA CATAGTTCAT CACAAGGAAT GAGAAATATA
    AAACATGTAG GTCCTTCTGG AAGATTTACT ATGAATATGC TGGTCGACAT TTTCTTGGAA
    TCTTTATCTT CTGATCCTGA GGTTTTGCTT CAAATTGATG GTGTTACTGA AGACAAACTG
    GAAAAATATG GTGCGGAAGT GATTTCAGTA TTACAGAAAT ACTCTGAATG GACATCGCCA
    GCTGAAGACA GTTCCCCAGG GATAAGCCTG TCCAGCAGCA GAGGCCCCGG AAGAAGTGCC
    GCTGAGGAGC TCGACGAGGA AATACCCGTA TCTTCCCACT ACTTTGCAAG TAAAACCAGA
    AATGAAAGGA AGAGGAAAAA GATGCCAGCC TCCCAAAGGT CTAAGAGGAG AAAAACTGCT
    TCCAGTGGTT CCAAGGCAAA GGGGGGGTCT GCCACATGTA GAAAGATATC TTCCAAAACG
    AAATCCTCCA GCATCATTGG ATCCAGTTCA GCCTCACATA CTTCTCAAGC GACATCAGGA
    GCCAATAGCA AATTGGGGAT TATGGCTCCA CCGAAGCCTA TAAATAGACC GTTTCTTAAG
    CCTTCATATG CATTCTCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001274176.1
    CDS100..3960
    Translation

    Target ORF information:

    RefSeq Version NM_001287247.2
    Organism Homo sapiens(human)
    Definition Homo sapiens BLM RecQ like helicase (BLM), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001287247.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
    CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA
    ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA
    AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC
    ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG
    AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC
    ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA
    TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT
    GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA
    AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA
    ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG
    ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT
    GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT
    CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA
    CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT
    GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT
    ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT
    CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC
    TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT
    AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG
    CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT
    GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG
    GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA
    AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT
    GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT
    GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC
    CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA
    AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC
    TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC
    ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT
    TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA
    GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA
    CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT
    AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG
    CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG
    CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC
    GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT
    AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA
    AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG
    GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC
    AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG
    TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC
    ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTGTTTA GCATGAGCTT TAACAGACAT
    AATCTGAAAT ACTATGTATT ACCGAAAAAG CCTAAAAAGG TGGCATTTGA TTGCCTAGAA
    TGGATCAGAA AGCACCACCC ATATGATTCA GGGATAATTT ACTGCCTCTC CAGGCGAGAA
    TGTGACACCA TGGCTGACAC GTTACAGAGA GATGGGCTCG CTGCTCTTGC TTACCATGCT
    GGCCTCAGTG ATTCTGCCAG AGATGAAGTG CAGCAGAAGT GGATTAATCA GGATGGCTGT
    CAGGTTATCT GTGCTACAAT TGCATTTGGA ATGGGGATTG ACAAACCGGA CGTGCGATTT
    GTGATTCATG CATCTCTCCC TAAATCTGTG GAGGGTTACT ACCAAGAATC TGGCAGAGCT
    GGAAGAGATG GGGAAATATC TCACTGCCTG CTTTTCTATA CCTATCATGA TGTGACCAGA
    CTGAAAAGAC TTATAATGAT GGAAAAAGAT GGAAACCATC ATACAAGAGA AACTCACTTC
    AATAATTTGT ATAGCATGGT ACATTACTGT GAAAATATAA CGGAATGCAG GAGAATACAG
    CTTTTGGCCT ACTTTGGTGA AAATGGATTT AATCCTGATT TTTGTAAGAA ACACCCAGAT
    GTTTCTTGTG ATAATTGCTG TAAAACAAAG GATTATAAAA CAAGAGATGT GACTGACGAT
    GTGAAAAGTA TTGTAAGATT TGTTCAAGAA CATAGTTCAT CACAAGGAAT GAGAAATATA
    AAACATGTAG GTCCTTCTGG AAGATTTACT ATGAATATGC TGGTCGACAT TTTCTTGGAA
    TCTTTATCTT CTGATCCTGA GGTTTTGCTT CAAATTGATG GTGTTACTGA AGACAAACTG
    GAAAAATATG GTGCGGAAGT GATTTCAGTA TTACAGAAAT ACTCTGAATG GACATCGCCA
    GCTGAAGACA GTTCCCCAGG GATAAGCCTG TCCAGCAGCA GAGGCCCCGG AAGAAGTGCC
    GCTGAGGAGC TCGACGAGGA AATACCCGTA TCTTCCCACT ACTTTGCAAG TAAAACCAGA
    AATGAAAGGA AGAGGAAAAA GATGCCAGCC TCCCAAAGGT CTAAGAGGAG AAAAACTGCT
    TCCAGTGGTT CCAAGGCAAA GGGGGGGTCT GCCACATGTA GAAAGATATC TTCCAAAACG
    AAATCCTCCA GCATCATTGG ATCCAGTTCA GCCTCACATA CTTCTCAAGC GACATCAGGA
    GCCAATAGCA AATTGGGGAT TATGGCTCCA CCGAAGCCTA TAAATAGACC GTTTCTTAAG
    CCTTCATATG CATTCTCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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