RHBDF1 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following RHBDF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RHBDF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu48918 XM_005255498.2
Latest version!
Homo sapiens rhomboid 5 homolog 1 (RHBDF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu03868 XM_005255494.1
Latest version!
Homo sapiens rhomboid 5 homolog 1 (RHBDF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $265.30
$379.00
OHu48916 XM_017023556.1
Latest version!
Homo sapiens rhomboid 5 homolog 1 (RHBDF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu48916 XM_006720921.1
Latest version!
Homo sapiens rhomboid 5 homolog 1 (RHBDF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu03868 NM_022450.5
Latest version!
Homo sapiens rhomboid 5 homolog 1 (RHBDF1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu86213 XM_017023557.1
Latest version!
Homo sapiens rhomboid 5 homolog 1 (RHBDF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu86214 XM_017023558.1
Latest version!
Homo sapiens rhomboid 5 homolog 1 (RHBDF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OHu03868 NM_022450.4 Homo sapiens rhomboid 5 homolog 1 (RHBDF1), mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu03868 NM_022450.3 Homo sapiens rhomboid 5 homolog 1 (RHBDF1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $265.30
$379.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu03868
    Clone ID Related Accession (Same CDS sequence) NM_022450.4 , XM_005255494.1 , NM_022450.5 , NM_022450.3
    Accession Version NM_022450.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2568bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-05
    Organism Homo sapiens(human)
    Product inactive rhomboid protein 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK291177.1, BM830007.1 and BC014425.1. On Nov 23, 2018 this sequence version replaced NM_022450.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC014425.1, AK026010.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
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    181
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    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAGTGAGG CCCGCAGGGA CAGCACGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTA 
    AAGCTGGACA TTCCCTCTGC GGTGCCCCTG ACGGCAGAAG AGCCCAGCTT CCTGCAGCCC
    CTGAGGCGAC AGGCTTTCCT GAGGAGTGTG AGTATGCCAG CCGAGACAGC CCACATCTCT
    TCACCCCACC ATGAGCTCCG GCGGCCGGTG CTGCAACGCC AGACGTCCAT CACACAGACC
    ATCCGCAGGG GGACCGCCGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAA
    TGGCAGCGCA AGAGCATCCG TCACTGCAGC CAGCGCTACG GGAAGCTGAA GCCCCAGGTC
    CTCCGGGAGC TGGACCTGCC CAGCCAGGAC AACGTGTCGC TGACCAGCAC CGAGACGCCA
    CCCCCACTCT ACGTGGGGCC ATGCCAGCTG GGCATGCAGA AGATCATAGA CCCCCTGGCC
    CGTGGCCGTG CCTTCCGTGT GGCAGATGAC ACTGCGGAAG GCCTGAGTGC CCCACACACT
    CCCGTCACGC CGGGTGCTGC CTCCCTCTGC TCCTTCTCCA GCTCCCGCTC AGGTTTCCAC
    CGGCTCCCGC GGCGGCGCAA GCGAGAGTCG GTGGCCAAGA TGAGCTTCCG GGCGGCCGCA
    GCGCTGATGA AAGGCCGCTC CGTTAGGGAT GGCACCTTTC GCCGGGCACA GCGTCGAAGC
    TTCACTCCAG CTAGCTTTCT GGAGGAGGAC ACAACTGATT TCCCCGATGA GCTGGACACA
    TCCTTCTTTG CCCGGGAAGG TATCCTCCAT GAAGAGCTGT CCACATACCC GGATGAAGTT
    TTCGAGTCCC CATCGGAGGC AGCGCTAAAG GACTGGGAGA AGGCACCGGA GCAGGCGGAC
    CTCACCGGCG GGGCCCTGGA CCGCAGCGAG CTTGAGCGCA GCCACCTGAT GCTGCCCTTG
    GAGCGAGGCT GGCGGAAGCA GAAGGAGGGC GCCGCAGCCC CGCAGCCCAA GGTGCGGCTC
    CGACAGGAGG TGGTGAGCAC CGCGGGGCCG CGACGGGGCC AGCGTATCGC GGTGCCGGTG
    CGCAAGCTCT TCGCCCGGGA GAAGCGGCCG TATGGGCTGG GCATGGTGGG ACGGCTCACC
    AACCGCACCT ACCGCAAGCG CATCGACAGC TTCGTCAAGC GCCAGATCGA GGACATGGAC
    GACCACAGGC CCTTCTTCAC CTACTGGCTT ACCTTCGTGC ACTCGCTCGT CACCATCCTA
    GCCGTGTGCA TCTATGGCAT CGCGCCCGTG GGCTTCTCGC AGCATGAGAC GGTGGACTCG
    GTGCTGCGGA ACCGCGGGGT CTACGAGAAC GTCAAGTACG TGCAGCAGGA GAACTTCTGG
    ATCGGGCCCA GCTCGGAGGC CCTCATCCAC CTGGGCGCCA AGTTTTCGCC CTGCATGCGC
    CAGGACCCGC AGGTGCACAG CTTCATTCGC TCGGCGCGCG AGCGCGAGAA GCACTCCGCC
    TGCTGCGTGC GCAACGACAG GTCGGGCTGC GTGCAGACCT CGGAGGAGGA GTGCTCGTCC
    ACGCTGGCAG TGTGGGTGAA GTGGCCCATC CATCCCAGCG CCCCAGAGCT TGCGGGCCAC
    AAGAGACAGT TTGGCTCTGT CTGCCACCAG GATCCCAGGG TGTGTGATGA GCCCTCCTCC
    GAAGACCCTC ATGAGTGGCC AGAAGACATC ACCAAGTGGC CGATCTGCAC CAAAAACAGC
    GCTGGGAACC ACACCAACCA TCCCCACATG GACTGTGTCA TCACAGGACG GCCCTGCTGC
    ATTGGCACCA AGGGCAGGTG TGAGATCACC TCCCGGGAGT ACTGTGACTT CATGAGGGGC
    TACTTCCATG AGGAGGCCAC GCTCTGCTCT CAGGTGCACT GCATGGATGA TGTGTGTGGG
    CTCCTGCCTT TTCTCAACCC CGAGGTGCCT GACCAGTTCT ACCGCCTGTG GCTATCCCTC
    TTCCTGCACG CCGGGATCTT GCACTGCCTG GTGTCCATCT GCTTCCAGAT GACTGTCCTG
    CGGGACCTGG AGAAGCTGGC AGGCTGGCAC CGCATAGCCA TCATCTACCT GCTGAGTGGT
    GTCACCGGCA ACCTGGCCAG TGCCATCTTC CTGCCATACC GAGCAGAGGT GGGTCCTGCT
    GGCTCCCAGT TCGGCATCCT GGCCTGCCTC TTCGTGGAGC TCTTCCAGAG CTGGCAGATC
    CTGGCGCGGC CCTGGCGTGC CTTCTTCAAG CTGCTGGCTG TGGTGCTCTT CCTCTTCACC
    TTTGGGCTGC TGCCGTGGAT TGACAACTTT GCCCACATCT CAGGGTTCAT CAGTGGCCTC
    TTCCTCTCCT TCGCCTTCTT GCCCTACATC AGCTTTGGCA AGTTCGACCT GTACCGGAAA
    CGCTGCCAGA TCATCATCTT TCAGGTGGTC TTCCTGGGCC TCCTGGCTGG CCTGGTGGTC
    CTCTTCTACG TCTATCCTGT CCGCTGTGAG TGGTGTGAGT TCCTCACCTG CATCCCCTTC
    ACTGACAAGT TCTGTGAGAA GTACGAACTG GACGCTCAGC TCCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_071895.3
    CDS149..2716
    Translation

    Target ORF information:

    RefSeq Version NM_022450.4
    Organism Homo sapiens(human)
    Definition Homo sapiens rhomboid 5 homolog 1 (RHBDF1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_022450.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAGTGAGG CCCGCAGGGA CAGCACGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTA 
    AAGCTGGACA TTCCCTCTGC GGTGCCCCTG ACGGCAGAAG AGCCCAGCTT CCTGCAGCCC
    CTGAGGCGAC AGGCTTTCCT GAGGAGTGTG AGTATGCCAG CCGAGACAGC CCACATCTCT
    TCACCCCACC ATGAGCTCCG GCGGCCGGTG CTGCAACGCC AGACGTCCAT CACACAGACC
    ATCCGCAGGG GGACCGCCGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAA
    TGGCAGCGCA AGAGCATCCG TCACTGCAGC CAGCGCTACG GGAAGCTGAA GCCCCAGGTC
    CTCCGGGAGC TGGACCTGCC CAGCCAGGAC AACGTGTCGC TGACCAGCAC CGAGACGCCA
    CCCCCACTCT ACGTGGGGCC ATGCCAGCTG GGCATGCAGA AGATCATAGA CCCCCTGGCC
    CGTGGCCGTG CCTTCCGTGT GGCAGATGAC ACTGCGGAAG GCCTGAGTGC CCCACACACT
    CCCGTCACGC CGGGTGCTGC CTCCCTCTGC TCCTTCTCCA GCTCCCGCTC AGGTTTCCAC
    CGGCTCCCGC GGCGGCGCAA GCGAGAGTCG GTGGCCAAGA TGAGCTTCCG GGCGGCCGCA
    GCGCTGATGA AAGGCCGCTC CGTTAGGGAT GGCACCTTTC GCCGGGCACA GCGTCGAAGC
    TTCACTCCAG CTAGCTTTCT GGAGGAGGAC ACAACTGATT TCCCCGATGA GCTGGACACA
    TCCTTCTTTG CCCGGGAAGG TATCCTCCAT GAAGAGCTGT CCACATACCC GGATGAAGTT
    TTCGAGTCCC CATCGGAGGC AGCGCTAAAG GACTGGGAGA AGGCACCGGA GCAGGCGGAC
    CTCACCGGCG GGGCCCTGGA CCGCAGCGAG CTTGAGCGCA GCCACCTGAT GCTGCCCTTG
    GAGCGAGGCT GGCGGAAGCA GAAGGAGGGC GCCGCAGCCC CGCAGCCCAA GGTGCGGCTC
    CGACAGGAGG TGGTGAGCAC CGCGGGGCCG CGACGGGGCC AGCGTATCGC GGTGCCGGTG
    CGCAAGCTCT TCGCCCGGGA GAAGCGGCCG TATGGGCTGG GCATGGTGGG ACGGCTCACC
    AACCGCACCT ACCGCAAGCG CATCGACAGC TTCGTCAAGC GCCAGATCGA GGACATGGAC
    GACCACAGGC CCTTCTTCAC CTACTGGCTT ACCTTCGTGC ACTCGCTCGT CACCATCCTA
    GCCGTGTGCA TCTATGGCAT CGCGCCCGTG GGCTTCTCGC AGCATGAGAC GGTGGACTCG
    GTGCTGCGGA ACCGCGGGGT CTACGAGAAC GTCAAGTACG TGCAGCAGGA GAACTTCTGG
    ATCGGGCCCA GCTCGGAGGC CCTCATCCAC CTGGGCGCCA AGTTTTCGCC CTGCATGCGC
    CAGGACCCGC AGGTGCACAG CTTCATTCGC TCGGCGCGCG AGCGCGAGAA GCACTCCGCC
    TGCTGCGTGC GCAACGACAG GTCGGGCTGC GTGCAGACCT CGGAGGAGGA GTGCTCGTCC
    ACGCTGGCAG TGTGGGTGAA GTGGCCCATC CATCCCAGCG CCCCAGAGCT TGCGGGCCAC
    AAGAGACAGT TTGGCTCTGT CTGCCACCAG GATCCCAGGG TGTGTGATGA GCCCTCCTCC
    GAAGACCCTC ATGAGTGGCC AGAAGACATC ACCAAGTGGC CGATCTGCAC CAAAAACAGC
    GCTGGGAACC ACACCAACCA TCCCCACATG GACTGTGTCA TCACAGGACG GCCCTGCTGC
    ATTGGCACCA AGGGCAGGTG TGAGATCACC TCCCGGGAGT ACTGTGACTT CATGAGGGGC
    TACTTCCATG AGGAGGCCAC GCTCTGCTCT CAGGTGCACT GCATGGATGA TGTGTGTGGG
    CTCCTGCCTT TTCTCAACCC CGAGGTGCCT GACCAGTTCT ACCGCCTGTG GCTATCCCTC
    TTCCTGCACG CCGGGATCTT GCACTGCCTG GTGTCCATCT GCTTCCAGAT GACTGTCCTG
    CGGGACCTGG AGAAGCTGGC AGGCTGGCAC CGCATAGCCA TCATCTACCT GCTGAGTGGT
    GTCACCGGCA ACCTGGCCAG TGCCATCTTC CTGCCATACC GAGCAGAGGT GGGTCCTGCT
    GGCTCCCAGT TCGGCATCCT GGCCTGCCTC TTCGTGGAGC TCTTCCAGAG CTGGCAGATC
    CTGGCGCGGC CCTGGCGTGC CTTCTTCAAG CTGCTGGCTG TGGTGCTCTT CCTCTTCACC
    TTTGGGCTGC TGCCGTGGAT TGACAACTTT GCCCACATCT CAGGGTTCAT CAGTGGCCTC
    TTCCTCTCCT TCGCCTTCTT GCCCTACATC AGCTTTGGCA AGTTCGACCT GTACCGGAAA
    CGCTGCCAGA TCATCATCTT TCAGGTGGTC TTCCTGGGCC TCCTGGCTGG CCTGGTGGTC
    CTCTTCTACG TCTATCCTGT CCGCTGTGAG TGGTGTGAGT TCCTCACCTG CATCCCCTTC
    ACTGACAAGT TCTGTGAGAA GTACGAACTG GACGCTCAGC TCCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu48918
    Clone ID Related Accession (Same CDS sequence) XM_005255498.2
    Accession Version XM_005255498.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1620bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product inactive rhomboid protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 3, 2014 this sequence version replaced XM_005255498.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCTGCCCT TGGAGCGAGG CTGGCGGAAG CAGAAGGAGG GCGCCGCAGC CCCGCAGCCC 
    AAGGTGCGGC TCCGACAGGA GGTGGTGAGC ACCGCGGGGC CGCGACGGGG CCAGCGTATC
    GCGGTGCCGG TGCGCAAGCT CTTCGCCCGG GAGAAGCGGC CGTATGGGCT GGGCATGGTG
    GGACGGCTCA CCAACCGCAC CTACCGCAAG CGCATCGACA GCTTCGTCAA GCGCCAGATC
    GAGGACATGG ACGACCACAG GCCCTTCTTC ACCTACTGGC TTACCTTCGT GCACTCGCTC
    GTCACCATCC TAGCCGTGTG CATCTATGGC ATCGCGCCCG TGGGCTTCTC GCAGCATGAG
    ACGGTGGACT CGGTGCTGCG GAACCGCGGG GTCTACGAGA ACGTCAAGTA CGTGCAGCAG
    GAGAACTTCT GGATCGGGCC CAGCTCGGAG GCCCTCATCC ACCTGGGCGC CAAGTTTTCG
    CCCTGCATGC GCCAGGACCC GCAGGTGCAC AGCTTCATTC GCTCGGCGCG CGAGCGCGAG
    AAGCACTCCG CCTGCTGCGT GCGCAACGAC AGGTCGGGCT GCGTGCAGAC CTCGGAGGAG
    GAGTGCTCGT CCACGCTGGC AGTGTGGGTG AAGTGGCCCA TCCATCCCAG CGCCCCAGAG
    CTTGCGGGCC ACAAGAGACA GTTTGGCTCT GTCTGCCACC AGGATCCCAG GGTGTGTGAT
    GAGCCCTCCT CCGAAGACCC TCATGAGTGG CCAGAAGACA TCACCAAGTG GCCGATCTGC
    ACCAAAAACA GCGCTGGGAA CCACACCAAC CATCCCCACA TGGACTGTGT CATCACAGGA
    CGGCCCTGCT GCATTGGCAC CAAGGGCAGG TGTGAGATCA CCTCCCGGGA GTACTGTGAC
    TTCATGAGGG GCTACTTCCA TGAGGAGGCC ACGCTCTGCT CTCAGGTGCA CTGCATGGAT
    GATGTGTGTG GGCTCCTGCC TTTTCTCAAC CCCGAGGTGC CTGACCAGTT CTACCGCCTG
    TGGCTATCCC TCTTCCTGCA CGCCGGGATC TTGCACTGCC TGGTGTCCAT CTGCTTCCAG
    ATGACTGTCC TGCGGGACCT GGAGAAGCTG GCAGGCTGGC ACCGCATAGC CATCATCTAC
    CTGCTGAGTG GTGTCACCGG CAACCTGGCC AGTGCCATCT TCCTGCCATA CCGAGCAGAG
    GTGGGTCCTG CTGGCTCCCA GTTCGGCATC CTGGCCTGCC TCTTCGTGGA GCTCTTCCAG
    AGCTGGCAGA TCCTGGCGCG GCCCTGGCGT GCCTTCTTCA AGCTGCTGGC TGTGGTGCTC
    TTCCTCTTCA CCTTTGGGCT GCTGCCGTGG ATTGACAACT TTGCCCACAT CTCAGGGTTC
    ATCAGTGGCC TCTTCCTCTC CTTCGCCTTC TTGCCCTACA TCAGCTTTGG CAAGTTCGAC
    CTGTACCGGA AACGCTGCCA GATCATCATC TTTCAGGTGG TCTTCCTGGG CCTCCTGGCT
    GGCCTGGTGG TCCTCTTCTA CGTCTATCCT GTCCGCTGTG AGTGGTGTGA GTTCCTCACC
    TGCATCCCCT TCACTGACAA GTTCTGTGAG AAGTACGAAC TGGACGCTCA GCTCCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005255555.1
    CDS947..2566
    Translation

    Target ORF information:

    RefSeq Version XM_005255498.2
    Organism Homo sapiens(human)
    Definition Homo sapiens rhomboid 5 homolog 1 (RHBDF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005255498.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCTGCCCT TGGAGCGAGG CTGGCGGAAG CAGAAGGAGG GCGCCGCAGC CCCGCAGCCC 
    AAGGTGCGGC TCCGACAGGA GGTGGTGAGC ACCGCGGGGC CGCGACGGGG CCAGCGTATC
    GCGGTGCCGG TGCGCAAGCT CTTCGCCCGG GAGAAGCGGC CGTATGGGCT GGGCATGGTG
    GGACGGCTCA CCAACCGCAC CTACCGCAAG CGCATCGACA GCTTCGTCAA GCGCCAGATC
    GAGGACATGG ACGACCACAG GCCCTTCTTC ACCTACTGGC TTACCTTCGT GCACTCGCTC
    GTCACCATCC TAGCCGTGTG CATCTATGGC ATCGCGCCCG TGGGCTTCTC GCAGCATGAG
    ACGGTGGACT CGGTGCTGCG GAACCGCGGG GTCTACGAGA ACGTCAAGTA CGTGCAGCAG
    GAGAACTTCT GGATCGGGCC CAGCTCGGAG GCCCTCATCC ACCTGGGCGC CAAGTTTTCG
    CCCTGCATGC GCCAGGACCC GCAGGTGCAC AGCTTCATTC GCTCGGCGCG CGAGCGCGAG
    AAGCACTCCG CCTGCTGCGT GCGCAACGAC AGGTCGGGCT GCGTGCAGAC CTCGGAGGAG
    GAGTGCTCGT CCACGCTGGC AGTGTGGGTG AAGTGGCCCA TCCATCCCAG CGCCCCAGAG
    CTTGCGGGCC ACAAGAGACA GTTTGGCTCT GTCTGCCACC AGGATCCCAG GGTGTGTGAT
    GAGCCCTCCT CCGAAGACCC TCATGAGTGG CCAGAAGACA TCACCAAGTG GCCGATCTGC
    ACCAAAAACA GCGCTGGGAA CCACACCAAC CATCCCCACA TGGACTGTGT CATCACAGGA
    CGGCCCTGCT GCATTGGCAC CAAGGGCAGG TGTGAGATCA CCTCCCGGGA GTACTGTGAC
    TTCATGAGGG GCTACTTCCA TGAGGAGGCC ACGCTCTGCT CTCAGGTGCA CTGCATGGAT
    GATGTGTGTG GGCTCCTGCC TTTTCTCAAC CCCGAGGTGC CTGACCAGTT CTACCGCCTG
    TGGCTATCCC TCTTCCTGCA CGCCGGGATC TTGCACTGCC TGGTGTCCAT CTGCTTCCAG
    ATGACTGTCC TGCGGGACCT GGAGAAGCTG GCAGGCTGGC ACCGCATAGC CATCATCTAC
    CTGCTGAGTG GTGTCACCGG CAACCTGGCC AGTGCCATCT TCCTGCCATA CCGAGCAGAG
    GTGGGTCCTG CTGGCTCCCA GTTCGGCATC CTGGCCTGCC TCTTCGTGGA GCTCTTCCAG
    AGCTGGCAGA TCCTGGCGCG GCCCTGGCGT GCCTTCTTCA AGCTGCTGGC TGTGGTGCTC
    TTCCTCTTCA CCTTTGGGCT GCTGCCGTGG ATTGACAACT TTGCCCACAT CTCAGGGTTC
    ATCAGTGGCC TCTTCCTCTC CTTCGCCTTC TTGCCCTACA TCAGCTTTGG CAAGTTCGAC
    CTGTACCGGA AACGCTGCCA GATCATCATC TTTCAGGTGG TCTTCCTGGG CCTCCTGGCT
    GGCCTGGTGG TCCTCTTCTA CGTCTATCCT GTCCGCTGTG AGTGGTGTGA GTTCCTCACC
    TGCATCCCCT TCACTGACAA GTTCTGTGAG AAGTACGAAC TGGACGCTCA GCTCCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu03868
    Clone ID Related Accession (Same CDS sequence) NM_022450.4 , XM_005255494.1 , NM_022450.5 , NM_022450.3
    Accession Version NM_022450.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2568bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-01
    Organism Homo sapiens(human)
    Product inactive rhomboid protein 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK291177.1, BM830007.1 and BC014425.1. On Jun 13, 2008 this sequence version replaced NM_022450.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC014425.1, AK026010.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAGTGAGG CCCGCAGGGA CAGCACGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTA 
    AAGCTGGACA TTCCCTCTGC GGTGCCCCTG ACGGCAGAAG AGCCCAGCTT CCTGCAGCCC
    CTGAGGCGAC AGGCTTTCCT GAGGAGTGTG AGTATGCCAG CCGAGACAGC CCACATCTCT
    TCACCCCACC ATGAGCTCCG GCGGCCGGTG CTGCAACGCC AGACGTCCAT CACACAGACC
    ATCCGCAGGG GGACCGCCGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAA
    TGGCAGCGCA AGAGCATCCG TCACTGCAGC CAGCGCTACG GGAAGCTGAA GCCCCAGGTC
    CTCCGGGAGC TGGACCTGCC CAGCCAGGAC AACGTGTCGC TGACCAGCAC CGAGACGCCA
    CCCCCACTCT ACGTGGGGCC ATGCCAGCTG GGCATGCAGA AGATCATAGA CCCCCTGGCC
    CGTGGCCGTG CCTTCCGTGT GGCAGATGAC ACTGCGGAAG GCCTGAGTGC CCCACACACT
    CCCGTCACGC CGGGTGCTGC CTCCCTCTGC TCCTTCTCCA GCTCCCGCTC AGGTTTCCAC
    CGGCTCCCGC GGCGGCGCAA GCGAGAGTCG GTGGCCAAGA TGAGCTTCCG GGCGGCCGCA
    GCGCTGATGA AAGGCCGCTC CGTTAGGGAT GGCACCTTTC GCCGGGCACA GCGTCGAAGC
    TTCACTCCAG CTAGCTTTCT GGAGGAGGAC ACAACTGATT TCCCCGATGA GCTGGACACA
    TCCTTCTTTG CCCGGGAAGG TATCCTCCAT GAAGAGCTGT CCACATACCC GGATGAAGTT
    TTCGAGTCCC CATCGGAGGC AGCGCTAAAG GACTGGGAGA AGGCACCGGA GCAGGCGGAC
    CTCACCGGCG GGGCCCTGGA CCGCAGCGAG CTTGAGCGCA GCCACCTGAT GCTGCCCTTG
    GAGCGAGGCT GGCGGAAGCA GAAGGAGGGC GCCGCAGCCC CGCAGCCCAA GGTGCGGCTC
    CGACAGGAGG TGGTGAGCAC CGCGGGGCCG CGACGGGGCC AGCGTATCGC GGTGCCGGTG
    CGCAAGCTCT TCGCCCGGGA GAAGCGGCCG TATGGGCTGG GCATGGTGGG ACGGCTCACC
    AACCGCACCT ACCGCAAGCG CATCGACAGC TTCGTCAAGC GCCAGATCGA GGACATGGAC
    GACCACAGGC CCTTCTTCAC CTACTGGCTT ACCTTCGTGC ACTCGCTCGT CACCATCCTA
    GCCGTGTGCA TCTATGGCAT CGCGCCCGTG GGCTTCTCGC AGCATGAGAC GGTGGACTCG
    GTGCTGCGGA ACCGCGGGGT CTACGAGAAC GTCAAGTACG TGCAGCAGGA GAACTTCTGG
    ATCGGGCCCA GCTCGGAGGC CCTCATCCAC CTGGGCGCCA AGTTTTCGCC CTGCATGCGC
    CAGGACCCGC AGGTGCACAG CTTCATTCGC TCGGCGCGCG AGCGCGAGAA GCACTCCGCC
    TGCTGCGTGC GCAACGACAG GTCGGGCTGC GTGCAGACCT CGGAGGAGGA GTGCTCGTCC
    ACGCTGGCAG TGTGGGTGAA GTGGCCCATC CATCCCAGCG CCCCAGAGCT TGCGGGCCAC
    AAGAGACAGT TTGGCTCTGT CTGCCACCAG GATCCCAGGG TGTGTGATGA GCCCTCCTCC
    GAAGACCCTC ATGAGTGGCC AGAAGACATC ACCAAGTGGC CGATCTGCAC CAAAAACAGC
    GCTGGGAACC ACACCAACCA TCCCCACATG GACTGTGTCA TCACAGGACG GCCCTGCTGC
    ATTGGCACCA AGGGCAGGTG TGAGATCACC TCCCGGGAGT ACTGTGACTT CATGAGGGGC
    TACTTCCATG AGGAGGCCAC GCTCTGCTCT CAGGTGCACT GCATGGATGA TGTGTGTGGG
    CTCCTGCCTT TTCTCAACCC CGAGGTGCCT GACCAGTTCT ACCGCCTGTG GCTATCCCTC
    TTCCTGCACG CCGGGATCTT GCACTGCCTG GTGTCCATCT GCTTCCAGAT GACTGTCCTG
    CGGGACCTGG AGAAGCTGGC AGGCTGGCAC CGCATAGCCA TCATCTACCT GCTGAGTGGT
    GTCACCGGCA ACCTGGCCAG TGCCATCTTC CTGCCATACC GAGCAGAGGT GGGTCCTGCT
    GGCTCCCAGT TCGGCATCCT GGCCTGCCTC TTCGTGGAGC TCTTCCAGAG CTGGCAGATC
    CTGGCGCGGC CCTGGCGTGC CTTCTTCAAG CTGCTGGCTG TGGTGCTCTT CCTCTTCACC
    TTTGGGCTGC TGCCGTGGAT TGACAACTTT GCCCACATCT CAGGGTTCAT CAGTGGCCTC
    TTCCTCTCCT TCGCCTTCTT GCCCTACATC AGCTTTGGCA AGTTCGACCT GTACCGGAAA
    CGCTGCCAGA TCATCATCTT TCAGGTGGTC TTCCTGGGCC TCCTGGCTGG CCTGGTGGTC
    CTCTTCTACG TCTATCCTGT CCGCTGTGAG TGGTGTGAGT TCCTCACCTG CATCCCCTTC
    ACTGACAAGT TCTGTGAGAA GTACGAACTG GACGCTCAGC TCCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_071895.3
    CDS149..2716
    Translation

    Target ORF information:

    RefSeq Version NM_022450.3
    Organism Homo sapiens(human)
    Definition Homo sapiens rhomboid 5 homolog 1 (RHBDF1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_022450.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAGTGAGG CCCGCAGGGA CAGCACGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTA 
    AAGCTGGACA TTCCCTCTGC GGTGCCCCTG ACGGCAGAAG AGCCCAGCTT CCTGCAGCCC
    CTGAGGCGAC AGGCTTTCCT GAGGAGTGTG AGTATGCCAG CCGAGACAGC CCACATCTCT
    TCACCCCACC ATGAGCTCCG GCGGCCGGTG CTGCAACGCC AGACGTCCAT CACACAGACC
    ATCCGCAGGG GGACCGCCGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAA
    TGGCAGCGCA AGAGCATCCG TCACTGCAGC CAGCGCTACG GGAAGCTGAA GCCCCAGGTC
    CTCCGGGAGC TGGACCTGCC CAGCCAGGAC AACGTGTCGC TGACCAGCAC CGAGACGCCA
    CCCCCACTCT ACGTGGGGCC ATGCCAGCTG GGCATGCAGA AGATCATAGA CCCCCTGGCC
    CGTGGCCGTG CCTTCCGTGT GGCAGATGAC ACTGCGGAAG GCCTGAGTGC CCCACACACT
    CCCGTCACGC CGGGTGCTGC CTCCCTCTGC TCCTTCTCCA GCTCCCGCTC AGGTTTCCAC
    CGGCTCCCGC GGCGGCGCAA GCGAGAGTCG GTGGCCAAGA TGAGCTTCCG GGCGGCCGCA
    GCGCTGATGA AAGGCCGCTC CGTTAGGGAT GGCACCTTTC GCCGGGCACA GCGTCGAAGC
    TTCACTCCAG CTAGCTTTCT GGAGGAGGAC ACAACTGATT TCCCCGATGA GCTGGACACA
    TCCTTCTTTG CCCGGGAAGG TATCCTCCAT GAAGAGCTGT CCACATACCC GGATGAAGTT
    TTCGAGTCCC CATCGGAGGC AGCGCTAAAG GACTGGGAGA AGGCACCGGA GCAGGCGGAC
    CTCACCGGCG GGGCCCTGGA CCGCAGCGAG CTTGAGCGCA GCCACCTGAT GCTGCCCTTG
    GAGCGAGGCT GGCGGAAGCA GAAGGAGGGC GCCGCAGCCC CGCAGCCCAA GGTGCGGCTC
    CGACAGGAGG TGGTGAGCAC CGCGGGGCCG CGACGGGGCC AGCGTATCGC GGTGCCGGTG
    CGCAAGCTCT TCGCCCGGGA GAAGCGGCCG TATGGGCTGG GCATGGTGGG ACGGCTCACC
    AACCGCACCT ACCGCAAGCG CATCGACAGC TTCGTCAAGC GCCAGATCGA GGACATGGAC
    GACCACAGGC CCTTCTTCAC CTACTGGCTT ACCTTCGTGC ACTCGCTCGT CACCATCCTA
    GCCGTGTGCA TCTATGGCAT CGCGCCCGTG GGCTTCTCGC AGCATGAGAC GGTGGACTCG
    GTGCTGCGGA ACCGCGGGGT CTACGAGAAC GTCAAGTACG TGCAGCAGGA GAACTTCTGG
    ATCGGGCCCA GCTCGGAGGC CCTCATCCAC CTGGGCGCCA AGTTTTCGCC CTGCATGCGC
    CAGGACCCGC AGGTGCACAG CTTCATTCGC TCGGCGCGCG AGCGCGAGAA GCACTCCGCC
    TGCTGCGTGC GCAACGACAG GTCGGGCTGC GTGCAGACCT CGGAGGAGGA GTGCTCGTCC
    ACGCTGGCAG TGTGGGTGAA GTGGCCCATC CATCCCAGCG CCCCAGAGCT TGCGGGCCAC
    AAGAGACAGT TTGGCTCTGT CTGCCACCAG GATCCCAGGG TGTGTGATGA GCCCTCCTCC
    GAAGACCCTC ATGAGTGGCC AGAAGACATC ACCAAGTGGC CGATCTGCAC CAAAAACAGC
    GCTGGGAACC ACACCAACCA TCCCCACATG GACTGTGTCA TCACAGGACG GCCCTGCTGC
    ATTGGCACCA AGGGCAGGTG TGAGATCACC TCCCGGGAGT ACTGTGACTT CATGAGGGGC
    TACTTCCATG AGGAGGCCAC GCTCTGCTCT CAGGTGCACT GCATGGATGA TGTGTGTGGG
    CTCCTGCCTT TTCTCAACCC CGAGGTGCCT GACCAGTTCT ACCGCCTGTG GCTATCCCTC
    TTCCTGCACG CCGGGATCTT GCACTGCCTG GTGTCCATCT GCTTCCAGAT GACTGTCCTG
    CGGGACCTGG AGAAGCTGGC AGGCTGGCAC CGCATAGCCA TCATCTACCT GCTGAGTGGT
    GTCACCGGCA ACCTGGCCAG TGCCATCTTC CTGCCATACC GAGCAGAGGT GGGTCCTGCT
    GGCTCCCAGT TCGGCATCCT GGCCTGCCTC TTCGTGGAGC TCTTCCAGAG CTGGCAGATC
    CTGGCGCGGC CCTGGCGTGC CTTCTTCAAG CTGCTGGCTG TGGTGCTCTT CCTCTTCACC
    TTTGGGCTGC TGCCGTGGAT TGACAACTTT GCCCACATCT CAGGGTTCAT CAGTGGCCTC
    TTCCTCTCCT TCGCCTTCTT GCCCTACATC AGCTTTGGCA AGTTCGACCT GTACCGGAAA
    CGCTGCCAGA TCATCATCTT TCAGGTGGTC TTCCTGGGCC TCCTGGCTGG CCTGGTGGTC
    CTCTTCTACG TCTATCCTGT CCGCTGTGAG TGGTGTGAGT TCCTCACCTG CATCCCCTTC
    ACTGACAAGT TCTGTGAGAA GTACGAACTG GACGCTCAGC TCCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu03868
    Clone ID Related Accession (Same CDS sequence) NM_022450.4 , XM_005255494.1 , NM_022450.5 , NM_022450.3
    Accession Version XM_005255494.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2568bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product inactive rhomboid protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAGTGAGG CCCGCAGGGA CAGCACGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTA 
    AAGCTGGACA TTCCCTCTGC GGTGCCCCTG ACGGCAGAAG AGCCCAGCTT CCTGCAGCCC
    CTGAGGCGAC AGGCTTTCCT GAGGAGTGTG AGTATGCCAG CCGAGACAGC CCACATCTCT
    TCACCCCACC ATGAGCTCCG GCGGCCGGTG CTGCAACGCC AGACGTCCAT CACACAGACC
    ATCCGCAGGG GGACCGCCGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAA
    TGGCAGCGCA AGAGCATCCG TCACTGCAGC CAGCGCTACG GGAAGCTGAA GCCCCAGGTC
    CTCCGGGAGC TGGACCTGCC CAGCCAGGAC AACGTGTCGC TGACCAGCAC CGAGACGCCA
    CCCCCACTCT ACGTGGGGCC ATGCCAGCTG GGCATGCAGA AGATCATAGA CCCCCTGGCC
    CGTGGCCGTG CCTTCCGTGT GGCAGATGAC ACTGCGGAAG GCCTGAGTGC CCCACACACT
    CCCGTCACGC CGGGTGCTGC CTCCCTCTGC TCCTTCTCCA GCTCCCGCTC AGGTTTCCAC
    CGGCTCCCGC GGCGGCGCAA GCGAGAGTCG GTGGCCAAGA TGAGCTTCCG GGCGGCCGCA
    GCGCTGATGA AAGGCCGCTC CGTTAGGGAT GGCACCTTTC GCCGGGCACA GCGTCGAAGC
    TTCACTCCAG CTAGCTTTCT GGAGGAGGAC ACAACTGATT TCCCCGATGA GCTGGACACA
    TCCTTCTTTG CCCGGGAAGG TATCCTCCAT GAAGAGCTGT CCACATACCC GGATGAAGTT
    TTCGAGTCCC CATCGGAGGC AGCGCTAAAG GACTGGGAGA AGGCACCGGA GCAGGCGGAC
    CTCACCGGCG GGGCCCTGGA CCGCAGCGAG CTTGAGCGCA GCCACCTGAT GCTGCCCTTG
    GAGCGAGGCT GGCGGAAGCA GAAGGAGGGC GCCGCAGCCC CGCAGCCCAA GGTGCGGCTC
    CGACAGGAGG TGGTGAGCAC CGCGGGGCCG CGACGGGGCC AGCGTATCGC GGTGCCGGTG
    CGCAAGCTCT TCGCCCGGGA GAAGCGGCCG TATGGGCTGG GCATGGTGGG ACGGCTCACC
    AACCGCACCT ACCGCAAGCG CATCGACAGC TTCGTCAAGC GCCAGATCGA GGACATGGAC
    GACCACAGGC CCTTCTTCAC CTACTGGCTT ACCTTCGTGC ACTCGCTCGT CACCATCCTA
    GCCGTGTGCA TCTATGGCAT CGCGCCCGTG GGCTTCTCGC AGCATGAGAC GGTGGACTCG
    GTGCTGCGGA ACCGCGGGGT CTACGAGAAC GTCAAGTACG TGCAGCAGGA GAACTTCTGG
    ATCGGGCCCA GCTCGGAGGC CCTCATCCAC CTGGGCGCCA AGTTTTCGCC CTGCATGCGC
    CAGGACCCGC AGGTGCACAG CTTCATTCGC TCGGCGCGCG AGCGCGAGAA GCACTCCGCC
    TGCTGCGTGC GCAACGACAG GTCGGGCTGC GTGCAGACCT CGGAGGAGGA GTGCTCGTCC
    ACGCTGGCAG TGTGGGTGAA GTGGCCCATC CATCCCAGCG CCCCAGAGCT TGCGGGCCAC
    AAGAGACAGT TTGGCTCTGT CTGCCACCAG GATCCCAGGG TGTGTGATGA GCCCTCCTCC
    GAAGACCCTC ATGAGTGGCC AGAAGACATC ACCAAGTGGC CGATCTGCAC CAAAAACAGC
    GCTGGGAACC ACACCAACCA TCCCCACATG GACTGTGTCA TCACAGGACG GCCCTGCTGC
    ATTGGCACCA AGGGCAGGTG TGAGATCACC TCCCGGGAGT ACTGTGACTT CATGAGGGGC
    TACTTCCATG AGGAGGCCAC GCTCTGCTCT CAGGTGCACT GCATGGATGA TGTGTGTGGG
    CTCCTGCCTT TTCTCAACCC CGAGGTGCCT GACCAGTTCT ACCGCCTGTG GCTATCCCTC
    TTCCTGCACG CCGGGATCTT GCACTGCCTG GTGTCCATCT GCTTCCAGAT GACTGTCCTG
    CGGGACCTGG AGAAGCTGGC AGGCTGGCAC CGCATAGCCA TCATCTACCT GCTGAGTGGT
    GTCACCGGCA ACCTGGCCAG TGCCATCTTC CTGCCATACC GAGCAGAGGT GGGTCCTGCT
    GGCTCCCAGT TCGGCATCCT GGCCTGCCTC TTCGTGGAGC TCTTCCAGAG CTGGCAGATC
    CTGGCGCGGC CCTGGCGTGC CTTCTTCAAG CTGCTGGCTG TGGTGCTCTT CCTCTTCACC
    TTTGGGCTGC TGCCGTGGAT TGACAACTTT GCCCACATCT CAGGGTTCAT CAGTGGCCTC
    TTCCTCTCCT TCGCCTTCTT GCCCTACATC AGCTTTGGCA AGTTCGACCT GTACCGGAAA
    CGCTGCCAGA TCATCATCTT TCAGGTGGTC TTCCTGGGCC TCCTGGCTGG CCTGGTGGTC
    CTCTTCTACG TCTATCCTGT CCGCTGTGAG TGGTGTGAGT TCCTCACCTG CATCCCCTTC
    ACTGACAAGT TCTGTGAGAA GTACGAACTG GACGCTCAGC TCCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005255551.1
    CDS128..2695
    Translation

    Target ORF information:

    RefSeq Version XM_005255494.1
    Organism Homo sapiens(human)
    Definition Homo sapiens rhomboid 5 homolog 1 (RHBDF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005255494.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAGTGAGG CCCGCAGGGA CAGCACGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTA 
    AAGCTGGACA TTCCCTCTGC GGTGCCCCTG ACGGCAGAAG AGCCCAGCTT CCTGCAGCCC
    CTGAGGCGAC AGGCTTTCCT GAGGAGTGTG AGTATGCCAG CCGAGACAGC CCACATCTCT
    TCACCCCACC ATGAGCTCCG GCGGCCGGTG CTGCAACGCC AGACGTCCAT CACACAGACC
    ATCCGCAGGG GGACCGCCGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAA
    TGGCAGCGCA AGAGCATCCG TCACTGCAGC CAGCGCTACG GGAAGCTGAA GCCCCAGGTC
    CTCCGGGAGC TGGACCTGCC CAGCCAGGAC AACGTGTCGC TGACCAGCAC CGAGACGCCA
    CCCCCACTCT ACGTGGGGCC ATGCCAGCTG GGCATGCAGA AGATCATAGA CCCCCTGGCC
    CGTGGCCGTG CCTTCCGTGT GGCAGATGAC ACTGCGGAAG GCCTGAGTGC CCCACACACT
    CCCGTCACGC CGGGTGCTGC CTCCCTCTGC TCCTTCTCCA GCTCCCGCTC AGGTTTCCAC
    CGGCTCCCGC GGCGGCGCAA GCGAGAGTCG GTGGCCAAGA TGAGCTTCCG GGCGGCCGCA
    GCGCTGATGA AAGGCCGCTC CGTTAGGGAT GGCACCTTTC GCCGGGCACA GCGTCGAAGC
    TTCACTCCAG CTAGCTTTCT GGAGGAGGAC ACAACTGATT TCCCCGATGA GCTGGACACA
    TCCTTCTTTG CCCGGGAAGG TATCCTCCAT GAAGAGCTGT CCACATACCC GGATGAAGTT
    TTCGAGTCCC CATCGGAGGC AGCGCTAAAG GACTGGGAGA AGGCACCGGA GCAGGCGGAC
    CTCACCGGCG GGGCCCTGGA CCGCAGCGAG CTTGAGCGCA GCCACCTGAT GCTGCCCTTG
    GAGCGAGGCT GGCGGAAGCA GAAGGAGGGC GCCGCAGCCC CGCAGCCCAA GGTGCGGCTC
    CGACAGGAGG TGGTGAGCAC CGCGGGGCCG CGACGGGGCC AGCGTATCGC GGTGCCGGTG
    CGCAAGCTCT TCGCCCGGGA GAAGCGGCCG TATGGGCTGG GCATGGTGGG ACGGCTCACC
    AACCGCACCT ACCGCAAGCG CATCGACAGC TTCGTCAAGC GCCAGATCGA GGACATGGAC
    GACCACAGGC CCTTCTTCAC CTACTGGCTT ACCTTCGTGC ACTCGCTCGT CACCATCCTA
    GCCGTGTGCA TCTATGGCAT CGCGCCCGTG GGCTTCTCGC AGCATGAGAC GGTGGACTCG
    GTGCTGCGGA ACCGCGGGGT CTACGAGAAC GTCAAGTACG TGCAGCAGGA GAACTTCTGG
    ATCGGGCCCA GCTCGGAGGC CCTCATCCAC CTGGGCGCCA AGTTTTCGCC CTGCATGCGC
    CAGGACCCGC AGGTGCACAG CTTCATTCGC TCGGCGCGCG AGCGCGAGAA GCACTCCGCC
    TGCTGCGTGC GCAACGACAG GTCGGGCTGC GTGCAGACCT CGGAGGAGGA GTGCTCGTCC
    ACGCTGGCAG TGTGGGTGAA GTGGCCCATC CATCCCAGCG CCCCAGAGCT TGCGGGCCAC
    AAGAGACAGT TTGGCTCTGT CTGCCACCAG GATCCCAGGG TGTGTGATGA GCCCTCCTCC
    GAAGACCCTC ATGAGTGGCC AGAAGACATC ACCAAGTGGC CGATCTGCAC CAAAAACAGC
    GCTGGGAACC ACACCAACCA TCCCCACATG GACTGTGTCA TCACAGGACG GCCCTGCTGC
    ATTGGCACCA AGGGCAGGTG TGAGATCACC TCCCGGGAGT ACTGTGACTT CATGAGGGGC
    TACTTCCATG AGGAGGCCAC GCTCTGCTCT CAGGTGCACT GCATGGATGA TGTGTGTGGG
    CTCCTGCCTT TTCTCAACCC CGAGGTGCCT GACCAGTTCT ACCGCCTGTG GCTATCCCTC
    TTCCTGCACG CCGGGATCTT GCACTGCCTG GTGTCCATCT GCTTCCAGAT GACTGTCCTG
    CGGGACCTGG AGAAGCTGGC AGGCTGGCAC CGCATAGCCA TCATCTACCT GCTGAGTGGT
    GTCACCGGCA ACCTGGCCAG TGCCATCTTC CTGCCATACC GAGCAGAGGT GGGTCCTGCT
    GGCTCCCAGT TCGGCATCCT GGCCTGCCTC TTCGTGGAGC TCTTCCAGAG CTGGCAGATC
    CTGGCGCGGC CCTGGCGTGC CTTCTTCAAG CTGCTGGCTG TGGTGCTCTT CCTCTTCACC
    TTTGGGCTGC TGCCGTGGAT TGACAACTTT GCCCACATCT CAGGGTTCAT CAGTGGCCTC
    TTCCTCTCCT TCGCCTTCTT GCCCTACATC AGCTTTGGCA AGTTCGACCT GTACCGGAAA
    CGCTGCCAGA TCATCATCTT TCAGGTGGTC TTCCTGGGCC TCCTGGCTGG CCTGGTGGTC
    CTCTTCTACG TCTATCCTGT CCGCTGTGAG TGGTGTGAGT TCCTCACCTG CATCCCCTTC
    ACTGACAAGT TCTGTGAGAA GTACGAACTG GACGCTCAGC TCCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu48916
    Clone ID Related Accession (Same CDS sequence) XM_017023556.1 , XM_006720921.1
    Accession Version XM_017023556.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2637bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product inactive rhomboid protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAGTGAGG CCCGCAGGGA CAGCACGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTA 
    AAGCTGGACA TTCCCTCTGC GGTGCCCCTG ACGGCAGAAG AGCCCAGCTT CCTGCAGGTA
    GGCCCTGGCC TGCAGGGACT GTGGGTGCCC CCTGTCCAGT ACCCTCACCA TGACCCTGTT
    GCCCAGCCCC TGAGGCGACA GGCTTTCCTG AGGAGTGTGA GTATGCCAGC CGAGACAGCC
    CACATCTCTT CACCCCACCA TGAGCTCCGG CGGCCGGTGC TGCAACGCCA GACGTCCATC
    ACACAGACCA TCCGCAGGGG GACCGCCGAC TGGTTTGGAG TGAGCAAGGA CAGTGACAGC
    ACCCAGAAAT GGCAGCGCAA GAGCATCCGT CACTGCAGCC AGCGCTACGG GAAGCTGAAG
    CCCCAGGTCC TCCGGGAGCT GGACCTGCCC AGCCAGGACA ACGTGTCGCT GACCAGCACC
    GAGACGCCAC CCCCACTCTA CGTGGGGCCA TGCCAGCTGG GCATGCAGAA GATCATAGAC
    CCCCTGGCCC GTGGCCGTGC CTTCCGTGTG GCAGATGACA CTGCGGAAGG CCTGAGTGCC
    CCACACACTC CCGTCACGCC GGGTGCTGCC TCCCTCTGCT CCTTCTCCAG CTCCCGCTCA
    GGTTTCCACC GGCTCCCGCG GCGGCGCAAG CGAGAGTCGG TGGCCAAGAT GAGCTTCCGG
    GCGGCCGCAG CGCTGATGAA AGGCCGCTCC GTTAGGGATG GCACCTTTCG CCGGGCACAG
    CGTCGAAGCT TCACTCCAGC TAGCTTTCTG GAGGAGGACA CAACTGATTT CCCCGATGAG
    CTGGACACAT CCTTCTTTGC CCGGGAAGGT ATCCTCCATG AAGAGCTGTC CACATACCCG
    GATGAAGTTT TCGAGTCCCC ATCGGAGGCA GCGCTAAAGG ACTGGGAGAA GGCACCGGAG
    CAGGCGGACC TCACCGGCGG GGCCCTGGAC CGCAGCGAGC TTGAGCGCAG CCACCTGATG
    CTGCCCTTGG AGCGAGGCTG GCGGAAGCAG AAGGAGGGCG CCGCAGCCCC GCAGCCCAAG
    GTGCGGCTCC GACAGGAGGT GGTGAGCACC GCGGGGCCGC GACGGGGCCA GCGTATCGCG
    GTGCCGGTGC GCAAGCTCTT CGCCCGGGAG AAGCGGCCGT ATGGGCTGGG CATGGTGGGA
    CGGCTCACCA ACCGCACCTA CCGCAAGCGC ATCGACAGCT TCGTCAAGCG CCAGATCGAG
    GACATGGACG ACCACAGGCC CTTCTTCACC TACTGGCTTA CCTTCGTGCA CTCGCTCGTC
    ACCATCCTAG CCGTGTGCAT CTATGGCATC GCGCCCGTGG GCTTCTCGCA GCATGAGACG
    GTGGACTCGG TGCTGCGGAA CCGCGGGGTC TACGAGAACG TCAAGTACGT GCAGCAGGAG
    AACTTCTGGA TCGGGCCCAG CTCGGAGGCC CTCATCCACC TGGGCGCCAA GTTTTCGCCC
    TGCATGCGCC AGGACCCGCA GGTGCACAGC TTCATTCGCT CGGCGCGCGA GCGCGAGAAG
    CACTCCGCCT GCTGCGTGCG CAACGACAGG TCGGGCTGCG TGCAGACCTC GGAGGAGGAG
    TGCTCGTCCA CGCTGGCAGT GTGGGTGAAG TGGCCCATCC ATCCCAGCGC CCCAGAGCTT
    GCGGGCCACA AGAGACAGTT TGGCTCTGTC TGCCACCAGG ATCCCAGGGT GTGTGATGAG
    CCCTCCTCCG AAGACCCTCA TGAGTGGCCA GAAGACATCA CCAAGTGGCC GATCTGCACC
    AAAAACAGCG CTGGGAACCA CACCAACCAT CCCCACATGG ACTGTGTCAT CACAGGACGG
    CCCTGCTGCA TTGGCACCAA GGGCAGGTGT GAGATCACCT CCCGGGAGTA CTGTGACTTC
    ATGAGGGGCT ACTTCCATGA GGAGGCCACG CTCTGCTCTC AGGTGCACTG CATGGATGAT
    GTGTGTGGGC TCCTGCCTTT TCTCAACCCC GAGGTGCCTG ACCAGTTCTA CCGCCTGTGG
    CTATCCCTCT TCCTGCACGC CGGGATCTTG CACTGCCTGG TGTCCATCTG CTTCCAGATG
    ACTGTCCTGC GGGACCTGGA GAAGCTGGCA GGCTGGCACC GCATAGCCAT CATCTACCTG
    CTGAGTGGTG TCACCGGCAA CCTGGCCAGT GCCATCTTCC TGCCATACCG AGCAGAGGTG
    GGTCCTGCTG GCTCCCAGTT CGGCATCCTG GCCTGCCTCT TCGTGGAGCT CTTCCAGAGC
    TGGCAGATCC TGGCGCGGCC CTGGCGTGCC TTCTTCAAGC TGCTGGCTGT GGTGCTCTTC
    CTCTTCACCT TTGGGCTGCT GCCGTGGATT GACAACTTTG CCCACATCTC AGGGTTCATC
    AGTGGCCTCT TCCTCTCCTT CGCCTTCTTG CCCTACATCA GCTTTGGCAA GTTCGACCTG
    TACCGGAAAC GCTGCCAGAT CATCATCTTT CAGGTGGTCT TCCTGGGCCT CCTGGCTGGC
    CTGGTGGTCC TCTTCTACGT CTATCCTGTC CGCTGTGAGT GGTGTGAGTT CCTCACCTGC
    ATCCCCTTCA CTGACAAGTT CTGTGAGAAG TACGAACTGG ACGCTCAGCT CCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016879045.1
    CDS114..2750
    Translation

    Target ORF information:

    RefSeq Version XM_017023556.1
    Organism Homo sapiens(human)
    Definition Homo sapiens rhomboid 5 homolog 1 (RHBDF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017023556.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAGTGAGG CCCGCAGGGA CAGCACGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTA 
    AAGCTGGACA TTCCCTCTGC GGTGCCCCTG ACGGCAGAAG AGCCCAGCTT CCTGCAGGTA
    GGCCCTGGCC TGCAGGGACT GTGGGTGCCC CCTGTCCAGT ACCCTCACCA TGACCCTGTT
    GCCCAGCCCC TGAGGCGACA GGCTTTCCTG AGGAGTGTGA GTATGCCAGC CGAGACAGCC
    CACATCTCTT CACCCCACCA TGAGCTCCGG CGGCCGGTGC TGCAACGCCA GACGTCCATC
    ACACAGACCA TCCGCAGGGG GACCGCCGAC TGGTTTGGAG TGAGCAAGGA CAGTGACAGC
    ACCCAGAAAT GGCAGCGCAA GAGCATCCGT CACTGCAGCC AGCGCTACGG GAAGCTGAAG
    CCCCAGGTCC TCCGGGAGCT GGACCTGCCC AGCCAGGACA ACGTGTCGCT GACCAGCACC
    GAGACGCCAC CCCCACTCTA CGTGGGGCCA TGCCAGCTGG GCATGCAGAA GATCATAGAC
    CCCCTGGCCC GTGGCCGTGC CTTCCGTGTG GCAGATGACA CTGCGGAAGG CCTGAGTGCC
    CCACACACTC CCGTCACGCC GGGTGCTGCC TCCCTCTGCT CCTTCTCCAG CTCCCGCTCA
    GGTTTCCACC GGCTCCCGCG GCGGCGCAAG CGAGAGTCGG TGGCCAAGAT GAGCTTCCGG
    GCGGCCGCAG CGCTGATGAA AGGCCGCTCC GTTAGGGATG GCACCTTTCG CCGGGCACAG
    CGTCGAAGCT TCACTCCAGC TAGCTTTCTG GAGGAGGACA CAACTGATTT CCCCGATGAG
    CTGGACACAT CCTTCTTTGC CCGGGAAGGT ATCCTCCATG AAGAGCTGTC CACATACCCG
    GATGAAGTTT TCGAGTCCCC ATCGGAGGCA GCGCTAAAGG ACTGGGAGAA GGCACCGGAG
    CAGGCGGACC TCACCGGCGG GGCCCTGGAC CGCAGCGAGC TTGAGCGCAG CCACCTGATG
    CTGCCCTTGG AGCGAGGCTG GCGGAAGCAG AAGGAGGGCG CCGCAGCCCC GCAGCCCAAG
    GTGCGGCTCC GACAGGAGGT GGTGAGCACC GCGGGGCCGC GACGGGGCCA GCGTATCGCG
    GTGCCGGTGC GCAAGCTCTT CGCCCGGGAG AAGCGGCCGT ATGGGCTGGG CATGGTGGGA
    CGGCTCACCA ACCGCACCTA CCGCAAGCGC ATCGACAGCT TCGTCAAGCG CCAGATCGAG
    GACATGGACG ACCACAGGCC CTTCTTCACC TACTGGCTTA CCTTCGTGCA CTCGCTCGTC
    ACCATCCTAG CCGTGTGCAT CTATGGCATC GCGCCCGTGG GCTTCTCGCA GCATGAGACG
    GTGGACTCGG TGCTGCGGAA CCGCGGGGTC TACGAGAACG TCAAGTACGT GCAGCAGGAG
    AACTTCTGGA TCGGGCCCAG CTCGGAGGCC CTCATCCACC TGGGCGCCAA GTTTTCGCCC
    TGCATGCGCC AGGACCCGCA GGTGCACAGC TTCATTCGCT CGGCGCGCGA GCGCGAGAAG
    CACTCCGCCT GCTGCGTGCG CAACGACAGG TCGGGCTGCG TGCAGACCTC GGAGGAGGAG
    TGCTCGTCCA CGCTGGCAGT GTGGGTGAAG TGGCCCATCC ATCCCAGCGC CCCAGAGCTT
    GCGGGCCACA AGAGACAGTT TGGCTCTGTC TGCCACCAGG ATCCCAGGGT GTGTGATGAG
    CCCTCCTCCG AAGACCCTCA TGAGTGGCCA GAAGACATCA CCAAGTGGCC GATCTGCACC
    AAAAACAGCG CTGGGAACCA CACCAACCAT CCCCACATGG ACTGTGTCAT CACAGGACGG
    CCCTGCTGCA TTGGCACCAA GGGCAGGTGT GAGATCACCT CCCGGGAGTA CTGTGACTTC
    ATGAGGGGCT ACTTCCATGA GGAGGCCACG CTCTGCTCTC AGGTGCACTG CATGGATGAT
    GTGTGTGGGC TCCTGCCTTT TCTCAACCCC GAGGTGCCTG ACCAGTTCTA CCGCCTGTGG
    CTATCCCTCT TCCTGCACGC CGGGATCTTG CACTGCCTGG TGTCCATCTG CTTCCAGATG
    ACTGTCCTGC GGGACCTGGA GAAGCTGGCA GGCTGGCACC GCATAGCCAT CATCTACCTG
    CTGAGTGGTG TCACCGGCAA CCTGGCCAGT GCCATCTTCC TGCCATACCG AGCAGAGGTG
    GGTCCTGCTG GCTCCCAGTT CGGCATCCTG GCCTGCCTCT TCGTGGAGCT CTTCCAGAGC
    TGGCAGATCC TGGCGCGGCC CTGGCGTGCC TTCTTCAAGC TGCTGGCTGT GGTGCTCTTC
    CTCTTCACCT TTGGGCTGCT GCCGTGGATT GACAACTTTG CCCACATCTC AGGGTTCATC
    AGTGGCCTCT TCCTCTCCTT CGCCTTCTTG CCCTACATCA GCTTTGGCAA GTTCGACCTG
    TACCGGAAAC GCTGCCAGAT CATCATCTTT CAGGTGGTCT TCCTGGGCCT CCTGGCTGGC
    CTGGTGGTCC TCTTCTACGT CTATCCTGTC CGCTGTGAGT GGTGTGAGTT CCTCACCTGC
    ATCCCCTTCA CTGACAAGTT CTGTGAGAAG TACGAACTGG ACGCTCAGCT CCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu48916
    Clone ID Related Accession (Same CDS sequence) XM_017023556.1 , XM_006720921.1
    Accession Version XM_006720921.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2637bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product inactive rhomboid protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAGTGAGG CCCGCAGGGA CAGCACGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTA 
    AAGCTGGACA TTCCCTCTGC GGTGCCCCTG ACGGCAGAAG AGCCCAGCTT CCTGCAGGTA
    GGCCCTGGCC TGCAGGGACT GTGGGTGCCC CCTGTCCAGT ACCCTCACCA TGACCCTGTT
    GCCCAGCCCC TGAGGCGACA GGCTTTCCTG AGGAGTGTGA GTATGCCAGC CGAGACAGCC
    CACATCTCTT CACCCCACCA TGAGCTCCGG CGGCCGGTGC TGCAACGCCA GACGTCCATC
    ACACAGACCA TCCGCAGGGG GACCGCCGAC TGGTTTGGAG TGAGCAAGGA CAGTGACAGC
    ACCCAGAAAT GGCAGCGCAA GAGCATCCGT CACTGCAGCC AGCGCTACGG GAAGCTGAAG
    CCCCAGGTCC TCCGGGAGCT GGACCTGCCC AGCCAGGACA ACGTGTCGCT GACCAGCACC
    GAGACGCCAC CCCCACTCTA CGTGGGGCCA TGCCAGCTGG GCATGCAGAA GATCATAGAC
    CCCCTGGCCC GTGGCCGTGC CTTCCGTGTG GCAGATGACA CTGCGGAAGG CCTGAGTGCC
    CCACACACTC CCGTCACGCC GGGTGCTGCC TCCCTCTGCT CCTTCTCCAG CTCCCGCTCA
    GGTTTCCACC GGCTCCCGCG GCGGCGCAAG CGAGAGTCGG TGGCCAAGAT GAGCTTCCGG
    GCGGCCGCAG CGCTGATGAA AGGCCGCTCC GTTAGGGATG GCACCTTTCG CCGGGCACAG
    CGTCGAAGCT TCACTCCAGC TAGCTTTCTG GAGGAGGACA CAACTGATTT CCCCGATGAG
    CTGGACACAT CCTTCTTTGC CCGGGAAGGT ATCCTCCATG AAGAGCTGTC CACATACCCG
    GATGAAGTTT TCGAGTCCCC ATCGGAGGCA GCGCTAAAGG ACTGGGAGAA GGCACCGGAG
    CAGGCGGACC TCACCGGCGG GGCCCTGGAC CGCAGCGAGC TTGAGCGCAG CCACCTGATG
    CTGCCCTTGG AGCGAGGCTG GCGGAAGCAG AAGGAGGGCG CCGCAGCCCC GCAGCCCAAG
    GTGCGGCTCC GACAGGAGGT GGTGAGCACC GCGGGGCCGC GACGGGGCCA GCGTATCGCG
    GTGCCGGTGC GCAAGCTCTT CGCCCGGGAG AAGCGGCCGT ATGGGCTGGG CATGGTGGGA
    CGGCTCACCA ACCGCACCTA CCGCAAGCGC ATCGACAGCT TCGTCAAGCG CCAGATCGAG
    GACATGGACG ACCACAGGCC CTTCTTCACC TACTGGCTTA CCTTCGTGCA CTCGCTCGTC
    ACCATCCTAG CCGTGTGCAT CTATGGCATC GCGCCCGTGG GCTTCTCGCA GCATGAGACG
    GTGGACTCGG TGCTGCGGAA CCGCGGGGTC TACGAGAACG TCAAGTACGT GCAGCAGGAG
    AACTTCTGGA TCGGGCCCAG CTCGGAGGCC CTCATCCACC TGGGCGCCAA GTTTTCGCCC
    TGCATGCGCC AGGACCCGCA GGTGCACAGC TTCATTCGCT CGGCGCGCGA GCGCGAGAAG
    CACTCCGCCT GCTGCGTGCG CAACGACAGG TCGGGCTGCG TGCAGACCTC GGAGGAGGAG
    TGCTCGTCCA CGCTGGCAGT GTGGGTGAAG TGGCCCATCC ATCCCAGCGC CCCAGAGCTT
    GCGGGCCACA AGAGACAGTT TGGCTCTGTC TGCCACCAGG ATCCCAGGGT GTGTGATGAG
    CCCTCCTCCG AAGACCCTCA TGAGTGGCCA GAAGACATCA CCAAGTGGCC GATCTGCACC
    AAAAACAGCG CTGGGAACCA CACCAACCAT CCCCACATGG ACTGTGTCAT CACAGGACGG
    CCCTGCTGCA TTGGCACCAA GGGCAGGTGT GAGATCACCT CCCGGGAGTA CTGTGACTTC
    ATGAGGGGCT ACTTCCATGA GGAGGCCACG CTCTGCTCTC AGGTGCACTG CATGGATGAT
    GTGTGTGGGC TCCTGCCTTT TCTCAACCCC GAGGTGCCTG ACCAGTTCTA CCGCCTGTGG
    CTATCCCTCT TCCTGCACGC CGGGATCTTG CACTGCCTGG TGTCCATCTG CTTCCAGATG
    ACTGTCCTGC GGGACCTGGA GAAGCTGGCA GGCTGGCACC GCATAGCCAT CATCTACCTG
    CTGAGTGGTG TCACCGGCAA CCTGGCCAGT GCCATCTTCC TGCCATACCG AGCAGAGGTG
    GGTCCTGCTG GCTCCCAGTT CGGCATCCTG GCCTGCCTCT TCGTGGAGCT CTTCCAGAGC
    TGGCAGATCC TGGCGCGGCC CTGGCGTGCC TTCTTCAAGC TGCTGGCTGT GGTGCTCTTC
    CTCTTCACCT TTGGGCTGCT GCCGTGGATT GACAACTTTG CCCACATCTC AGGGTTCATC
    AGTGGCCTCT TCCTCTCCTT CGCCTTCTTG CCCTACATCA GCTTTGGCAA GTTCGACCTG
    TACCGGAAAC GCTGCCAGAT CATCATCTTT CAGGTGGTCT TCCTGGGCCT CCTGGCTGGC
    CTGGTGGTCC TCTTCTACGT CTATCCTGTC CGCTGTGAGT GGTGTGAGTT CCTCACCTGC
    ATCCCCTTCA CTGACAAGTT CTGTGAGAAG TACGAACTGG ACGCTCAGCT CCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006720984.1
    CDS142..2778
    Translation

    Target ORF information:

    RefSeq Version XM_006720921.1
    Organism Homo sapiens(human)
    Definition Homo sapiens rhomboid 5 homolog 1 (RHBDF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006720921.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAGTGAGG CCCGCAGGGA CAGCACGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTA 
    AAGCTGGACA TTCCCTCTGC GGTGCCCCTG ACGGCAGAAG AGCCCAGCTT CCTGCAGGTA
    GGCCCTGGCC TGCAGGGACT GTGGGTGCCC CCTGTCCAGT ACCCTCACCA TGACCCTGTT
    GCCCAGCCCC TGAGGCGACA GGCTTTCCTG AGGAGTGTGA GTATGCCAGC CGAGACAGCC
    CACATCTCTT CACCCCACCA TGAGCTCCGG CGGCCGGTGC TGCAACGCCA GACGTCCATC
    ACACAGACCA TCCGCAGGGG GACCGCCGAC TGGTTTGGAG TGAGCAAGGA CAGTGACAGC
    ACCCAGAAAT GGCAGCGCAA GAGCATCCGT CACTGCAGCC AGCGCTACGG GAAGCTGAAG
    CCCCAGGTCC TCCGGGAGCT GGACCTGCCC AGCCAGGACA ACGTGTCGCT GACCAGCACC
    GAGACGCCAC CCCCACTCTA CGTGGGGCCA TGCCAGCTGG GCATGCAGAA GATCATAGAC
    CCCCTGGCCC GTGGCCGTGC CTTCCGTGTG GCAGATGACA CTGCGGAAGG CCTGAGTGCC
    CCACACACTC CCGTCACGCC GGGTGCTGCC TCCCTCTGCT CCTTCTCCAG CTCCCGCTCA
    GGTTTCCACC GGCTCCCGCG GCGGCGCAAG CGAGAGTCGG TGGCCAAGAT GAGCTTCCGG
    GCGGCCGCAG CGCTGATGAA AGGCCGCTCC GTTAGGGATG GCACCTTTCG CCGGGCACAG
    CGTCGAAGCT TCACTCCAGC TAGCTTTCTG GAGGAGGACA CAACTGATTT CCCCGATGAG
    CTGGACACAT CCTTCTTTGC CCGGGAAGGT ATCCTCCATG AAGAGCTGTC CACATACCCG
    GATGAAGTTT TCGAGTCCCC ATCGGAGGCA GCGCTAAAGG ACTGGGAGAA GGCACCGGAG
    CAGGCGGACC TCACCGGCGG GGCCCTGGAC CGCAGCGAGC TTGAGCGCAG CCACCTGATG
    CTGCCCTTGG AGCGAGGCTG GCGGAAGCAG AAGGAGGGCG CCGCAGCCCC GCAGCCCAAG
    GTGCGGCTCC GACAGGAGGT GGTGAGCACC GCGGGGCCGC GACGGGGCCA GCGTATCGCG
    GTGCCGGTGC GCAAGCTCTT CGCCCGGGAG AAGCGGCCGT ATGGGCTGGG CATGGTGGGA
    CGGCTCACCA ACCGCACCTA CCGCAAGCGC ATCGACAGCT TCGTCAAGCG CCAGATCGAG
    GACATGGACG ACCACAGGCC CTTCTTCACC TACTGGCTTA CCTTCGTGCA CTCGCTCGTC
    ACCATCCTAG CCGTGTGCAT CTATGGCATC GCGCCCGTGG GCTTCTCGCA GCATGAGACG
    GTGGACTCGG TGCTGCGGAA CCGCGGGGTC TACGAGAACG TCAAGTACGT GCAGCAGGAG
    AACTTCTGGA TCGGGCCCAG CTCGGAGGCC CTCATCCACC TGGGCGCCAA GTTTTCGCCC
    TGCATGCGCC AGGACCCGCA GGTGCACAGC TTCATTCGCT CGGCGCGCGA GCGCGAGAAG
    CACTCCGCCT GCTGCGTGCG CAACGACAGG TCGGGCTGCG TGCAGACCTC GGAGGAGGAG
    TGCTCGTCCA CGCTGGCAGT GTGGGTGAAG TGGCCCATCC ATCCCAGCGC CCCAGAGCTT
    GCGGGCCACA AGAGACAGTT TGGCTCTGTC TGCCACCAGG ATCCCAGGGT GTGTGATGAG
    CCCTCCTCCG AAGACCCTCA TGAGTGGCCA GAAGACATCA CCAAGTGGCC GATCTGCACC
    AAAAACAGCG CTGGGAACCA CACCAACCAT CCCCACATGG ACTGTGTCAT CACAGGACGG
    CCCTGCTGCA TTGGCACCAA GGGCAGGTGT GAGATCACCT CCCGGGAGTA CTGTGACTTC
    ATGAGGGGCT ACTTCCATGA GGAGGCCACG CTCTGCTCTC AGGTGCACTG CATGGATGAT
    GTGTGTGGGC TCCTGCCTTT TCTCAACCCC GAGGTGCCTG ACCAGTTCTA CCGCCTGTGG
    CTATCCCTCT TCCTGCACGC CGGGATCTTG CACTGCCTGG TGTCCATCTG CTTCCAGATG
    ACTGTCCTGC GGGACCTGGA GAAGCTGGCA GGCTGGCACC GCATAGCCAT CATCTACCTG
    CTGAGTGGTG TCACCGGCAA CCTGGCCAGT GCCATCTTCC TGCCATACCG AGCAGAGGTG
    GGTCCTGCTG GCTCCCAGTT CGGCATCCTG GCCTGCCTCT TCGTGGAGCT CTTCCAGAGC
    TGGCAGATCC TGGCGCGGCC CTGGCGTGCC TTCTTCAAGC TGCTGGCTGT GGTGCTCTTC
    CTCTTCACCT TTGGGCTGCT GCCGTGGATT GACAACTTTG CCCACATCTC AGGGTTCATC
    AGTGGCCTCT TCCTCTCCTT CGCCTTCTTG CCCTACATCA GCTTTGGCAA GTTCGACCTG
    TACCGGAAAC GCTGCCAGAT CATCATCTTT CAGGTGGTCT TCCTGGGCCT CCTGGCTGGC
    CTGGTGGTCC TCTTCTACGT CTATCCTGTC CGCTGTGAGT GGTGTGAGTT CCTCACCTGC
    ATCCCCTTCA CTGACAAGTT CTGTGAGAAG TACGAACTGG ACGCTCAGCT CCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu03868
    Clone ID Related Accession (Same CDS sequence) NM_022450.4 , XM_005255494.1 , NM_022450.5 , NM_022450.3
    Accession Version NM_022450.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2568bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product inactive rhomboid protein 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from Z69720.1, Z69719.1, KF511226.1 and KF511222.1. On Aug 31, 2019 this sequence version replaced NM_022450.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC014425.1, AK291177.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000262316.10/ ENSP00000262316.5 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAGTGAGG CCCGCAGGGA CAGCACGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTA 
    AAGCTGGACA TTCCCTCTGC GGTGCCCCTG ACGGCAGAAG AGCCCAGCTT CCTGCAGCCC
    CTGAGGCGAC AGGCTTTCCT GAGGAGTGTG AGTATGCCAG CCGAGACAGC CCACATCTCT
    TCACCCCACC ATGAGCTCCG GCGGCCGGTG CTGCAACGCC AGACGTCCAT CACACAGACC
    ATCCGCAGGG GGACCGCCGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAA
    TGGCAGCGCA AGAGCATCCG TCACTGCAGC CAGCGCTACG GGAAGCTGAA GCCCCAGGTC
    CTCCGGGAGC TGGACCTGCC CAGCCAGGAC AACGTGTCGC TGACCAGCAC CGAGACGCCA
    CCCCCACTCT ACGTGGGGCC ATGCCAGCTG GGCATGCAGA AGATCATAGA CCCCCTGGCC
    CGTGGCCGTG CCTTCCGTGT GGCAGATGAC ACTGCGGAAG GCCTGAGTGC CCCACACACT
    CCCGTCACGC CGGGTGCTGC CTCCCTCTGC TCCTTCTCCA GCTCCCGCTC AGGTTTCCAC
    CGGCTCCCGC GGCGGCGCAA GCGAGAGTCG GTGGCCAAGA TGAGCTTCCG GGCGGCCGCA
    GCGCTGATGA AAGGCCGCTC CGTTAGGGAT GGCACCTTTC GCCGGGCACA GCGTCGAAGC
    TTCACTCCAG CTAGCTTTCT GGAGGAGGAC ACAACTGATT TCCCCGATGA GCTGGACACA
    TCCTTCTTTG CCCGGGAAGG TATCCTCCAT GAAGAGCTGT CCACATACCC GGATGAAGTT
    TTCGAGTCCC CATCGGAGGC AGCGCTAAAG GACTGGGAGA AGGCACCGGA GCAGGCGGAC
    CTCACCGGCG GGGCCCTGGA CCGCAGCGAG CTTGAGCGCA GCCACCTGAT GCTGCCCTTG
    GAGCGAGGCT GGCGGAAGCA GAAGGAGGGC GCCGCAGCCC CGCAGCCCAA GGTGCGGCTC
    CGACAGGAGG TGGTGAGCAC CGCGGGGCCG CGACGGGGCC AGCGTATCGC GGTGCCGGTG
    CGCAAGCTCT TCGCCCGGGA GAAGCGGCCG TATGGGCTGG GCATGGTGGG ACGGCTCACC
    AACCGCACCT ACCGCAAGCG CATCGACAGC TTCGTCAAGC GCCAGATCGA GGACATGGAC
    GACCACAGGC CCTTCTTCAC CTACTGGCTT ACCTTCGTGC ACTCGCTCGT CACCATCCTA
    GCCGTGTGCA TCTATGGCAT CGCGCCCGTG GGCTTCTCGC AGCATGAGAC GGTGGACTCG
    GTGCTGCGGA ACCGCGGGGT CTACGAGAAC GTCAAGTACG TGCAGCAGGA GAACTTCTGG
    ATCGGGCCCA GCTCGGAGGC CCTCATCCAC CTGGGCGCCA AGTTTTCGCC CTGCATGCGC
    CAGGACCCGC AGGTGCACAG CTTCATTCGC TCGGCGCGCG AGCGCGAGAA GCACTCCGCC
    TGCTGCGTGC GCAACGACAG GTCGGGCTGC GTGCAGACCT CGGAGGAGGA GTGCTCGTCC
    ACGCTGGCAG TGTGGGTGAA GTGGCCCATC CATCCCAGCG CCCCAGAGCT TGCGGGCCAC
    AAGAGACAGT TTGGCTCTGT CTGCCACCAG GATCCCAGGG TGTGTGATGA GCCCTCCTCC
    GAAGACCCTC ATGAGTGGCC AGAAGACATC ACCAAGTGGC CGATCTGCAC CAAAAACAGC
    GCTGGGAACC ACACCAACCA TCCCCACATG GACTGTGTCA TCACAGGACG GCCCTGCTGC
    ATTGGCACCA AGGGCAGGTG TGAGATCACC TCCCGGGAGT ACTGTGACTT CATGAGGGGC
    TACTTCCATG AGGAGGCCAC GCTCTGCTCT CAGGTGCACT GCATGGATGA TGTGTGTGGG
    CTCCTGCCTT TTCTCAACCC CGAGGTGCCT GACCAGTTCT ACCGCCTGTG GCTATCCCTC
    TTCCTGCACG CCGGGATCTT GCACTGCCTG GTGTCCATCT GCTTCCAGAT GACTGTCCTG
    CGGGACCTGG AGAAGCTGGC AGGCTGGCAC CGCATAGCCA TCATCTACCT GCTGAGTGGT
    GTCACCGGCA ACCTGGCCAG TGCCATCTTC CTGCCATACC GAGCAGAGGT GGGTCCTGCT
    GGCTCCCAGT TCGGCATCCT GGCCTGCCTC TTCGTGGAGC TCTTCCAGAG CTGGCAGATC
    CTGGCGCGGC CCTGGCGTGC CTTCTTCAAG CTGCTGGCTG TGGTGCTCTT CCTCTTCACC
    TTTGGGCTGC TGCCGTGGAT TGACAACTTT GCCCACATCT CAGGGTTCAT CAGTGGCCTC
    TTCCTCTCCT TCGCCTTCTT GCCCTACATC AGCTTTGGCA AGTTCGACCT GTACCGGAAA
    CGCTGCCAGA TCATCATCTT TCAGGTGGTC TTCCTGGGCC TCCTGGCTGG CCTGGTGGTC
    CTCTTCTACG TCTATCCTGT CCGCTGTGAG TGGTGTGAGT TCCTCACCTG CATCCCCTTC
    ACTGACAAGT TCTGTGAGAA GTACGAACTG GACGCTCAGC TCCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_071895.3
    CDS144..2711
    Translation

    Target ORF information:

    RefSeq Version NM_022450.5
    Organism Homo sapiens(human)
    Definition Homo sapiens rhomboid 5 homolog 1 (RHBDF1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_022450.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAGTGAGG CCCGCAGGGA CAGCACGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTA 
    AAGCTGGACA TTCCCTCTGC GGTGCCCCTG ACGGCAGAAG AGCCCAGCTT CCTGCAGCCC
    CTGAGGCGAC AGGCTTTCCT GAGGAGTGTG AGTATGCCAG CCGAGACAGC CCACATCTCT
    TCACCCCACC ATGAGCTCCG GCGGCCGGTG CTGCAACGCC AGACGTCCAT CACACAGACC
    ATCCGCAGGG GGACCGCCGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAA
    TGGCAGCGCA AGAGCATCCG TCACTGCAGC CAGCGCTACG GGAAGCTGAA GCCCCAGGTC
    CTCCGGGAGC TGGACCTGCC CAGCCAGGAC AACGTGTCGC TGACCAGCAC CGAGACGCCA
    CCCCCACTCT ACGTGGGGCC ATGCCAGCTG GGCATGCAGA AGATCATAGA CCCCCTGGCC
    CGTGGCCGTG CCTTCCGTGT GGCAGATGAC ACTGCGGAAG GCCTGAGTGC CCCACACACT
    CCCGTCACGC CGGGTGCTGC CTCCCTCTGC TCCTTCTCCA GCTCCCGCTC AGGTTTCCAC
    CGGCTCCCGC GGCGGCGCAA GCGAGAGTCG GTGGCCAAGA TGAGCTTCCG GGCGGCCGCA
    GCGCTGATGA AAGGCCGCTC CGTTAGGGAT GGCACCTTTC GCCGGGCACA GCGTCGAAGC
    TTCACTCCAG CTAGCTTTCT GGAGGAGGAC ACAACTGATT TCCCCGATGA GCTGGACACA
    TCCTTCTTTG CCCGGGAAGG TATCCTCCAT GAAGAGCTGT CCACATACCC GGATGAAGTT
    TTCGAGTCCC CATCGGAGGC AGCGCTAAAG GACTGGGAGA AGGCACCGGA GCAGGCGGAC
    CTCACCGGCG GGGCCCTGGA CCGCAGCGAG CTTGAGCGCA GCCACCTGAT GCTGCCCTTG
    GAGCGAGGCT GGCGGAAGCA GAAGGAGGGC GCCGCAGCCC CGCAGCCCAA GGTGCGGCTC
    CGACAGGAGG TGGTGAGCAC CGCGGGGCCG CGACGGGGCC AGCGTATCGC GGTGCCGGTG
    CGCAAGCTCT TCGCCCGGGA GAAGCGGCCG TATGGGCTGG GCATGGTGGG ACGGCTCACC
    AACCGCACCT ACCGCAAGCG CATCGACAGC TTCGTCAAGC GCCAGATCGA GGACATGGAC
    GACCACAGGC CCTTCTTCAC CTACTGGCTT ACCTTCGTGC ACTCGCTCGT CACCATCCTA
    GCCGTGTGCA TCTATGGCAT CGCGCCCGTG GGCTTCTCGC AGCATGAGAC GGTGGACTCG
    GTGCTGCGGA ACCGCGGGGT CTACGAGAAC GTCAAGTACG TGCAGCAGGA GAACTTCTGG
    ATCGGGCCCA GCTCGGAGGC CCTCATCCAC CTGGGCGCCA AGTTTTCGCC CTGCATGCGC
    CAGGACCCGC AGGTGCACAG CTTCATTCGC TCGGCGCGCG AGCGCGAGAA GCACTCCGCC
    TGCTGCGTGC GCAACGACAG GTCGGGCTGC GTGCAGACCT CGGAGGAGGA GTGCTCGTCC
    ACGCTGGCAG TGTGGGTGAA GTGGCCCATC CATCCCAGCG CCCCAGAGCT TGCGGGCCAC
    AAGAGACAGT TTGGCTCTGT CTGCCACCAG GATCCCAGGG TGTGTGATGA GCCCTCCTCC
    GAAGACCCTC ATGAGTGGCC AGAAGACATC ACCAAGTGGC CGATCTGCAC CAAAAACAGC
    GCTGGGAACC ACACCAACCA TCCCCACATG GACTGTGTCA TCACAGGACG GCCCTGCTGC
    ATTGGCACCA AGGGCAGGTG TGAGATCACC TCCCGGGAGT ACTGTGACTT CATGAGGGGC
    TACTTCCATG AGGAGGCCAC GCTCTGCTCT CAGGTGCACT GCATGGATGA TGTGTGTGGG
    CTCCTGCCTT TTCTCAACCC CGAGGTGCCT GACCAGTTCT ACCGCCTGTG GCTATCCCTC
    TTCCTGCACG CCGGGATCTT GCACTGCCTG GTGTCCATCT GCTTCCAGAT GACTGTCCTG
    CGGGACCTGG AGAAGCTGGC AGGCTGGCAC CGCATAGCCA TCATCTACCT GCTGAGTGGT
    GTCACCGGCA ACCTGGCCAG TGCCATCTTC CTGCCATACC GAGCAGAGGT GGGTCCTGCT
    GGCTCCCAGT TCGGCATCCT GGCCTGCCTC TTCGTGGAGC TCTTCCAGAG CTGGCAGATC
    CTGGCGCGGC CCTGGCGTGC CTTCTTCAAG CTGCTGGCTG TGGTGCTCTT CCTCTTCACC
    TTTGGGCTGC TGCCGTGGAT TGACAACTTT GCCCACATCT CAGGGTTCAT CAGTGGCCTC
    TTCCTCTCCT TCGCCTTCTT GCCCTACATC AGCTTTGGCA AGTTCGACCT GTACCGGAAA
    CGCTGCCAGA TCATCATCTT TCAGGTGGTC TTCCTGGGCC TCCTGGCTGG CCTGGTGGTC
    CTCTTCTACG TCTATCCTGT CCGCTGTGAG TGGTGTGAGT TCCTCACCTG CATCCCCTTC
    ACTGACAAGT TCTGTGAGAA GTACGAACTG GACGCTCAGC TCCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu86213
    Clone ID Related Accession (Same CDS sequence) XM_017023557.1
    Accession Version XM_017023557.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2475bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product inactive rhomboid protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGAGTGAGG CCCGCAGGGA CAGCACGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTA 
    AAGCTGGACA TTCCCTCTGC GGTGCCCCTG ACGGCAGAAG AGCCCAGCTT CCTGCAGGTA
    GGCCCTGGCC TGCAGGGACT GTGGGTGCCC CCTGTCCAGT ACCCTCACCA TGACCCTGTT
    GCCCAGCCCC TGAGGCGACA GGCTTTCCTG AGGAGTGTGA GTATGCCAGC CGAGACAGCC
    CACATCTCTT CACCCCACCA TGAGCTCCGG CGGCCGGTGC TGCAACGCCA GACGTCCATC
    ACACAGACCA TCCGCAGGGG GACCGCCGAC TGGTTTGGAG TGAGCAAGGA CAGTGACAGC
    ACCCAGAAAT GGCAGCGCAA GAGCATCCGT CACTGCAGCC AGCGCTACGG GAAGCTGAAG
    CCCCAGGTCC TCCGGGAGCT GGACCTGCCC AGCCAGGACA ACGTGTCGCT GACCAGCACC
    GAGACGCCAC CCCCACTCTA CGTGGGGCCA TGCCAGCTGG GCATGCAGAA GATCATAGAC
    CCCCTGGCCC GTGGCCGTGC CTTCCGTGTG GCAGATGACA CTGCGGAAGG CCTGAGTGCC
    CCACACACTC CCGTCACGCC GGGTGCTGCC TCCCTCTGCT CCTTCTCCAG CTCCCGCTCA
    GGTTTCCACC GGCTCCCGCG GCGGCGCAAG CGAGAGTCGG TGGCCAAGAT GAGCTTCCGG
    GCGGCCGCAG CGCTGATGAA AGGCCGCTCC GTTAGGGATG GCACCTTTCG CCGGGCACAG
    CGTCGAAGCT TCACTCCAGC TAGCTTTCTG GAGGAGGACA CAACTGATTT CCCCGATGAG
    CTGGACACAT CCTTCTTTGC CCGGGAAGGT ATCCTCCATG AAGAGCTGTC CACATACCCG
    GATGAAGTTT TCGAGTCCCC ATCGGAGGCA GCGCTAAAGG ACTGGGAGAA GGCACCGGAG
    CAGGCGGACC TCACCGGCGG GGCCCTGGAC CGCAGCGAGC TTGAGCGCAG CCACCTGATG
    CTGCCCTTGG AGCGAGGCTG GCGGAAGCAG AAGGAGGGCG CCGCAGCCCC GCAGCCCAAG
    GTGCGGCTCC GACAGGAGGT GGTGAGCACC GCGGGGCCGC GACGGGGCCA GCGTATCGCG
    GTGCCGGTGC GCAAGCTCTT CGCCCGGGAG AAGCGGCCGT ATGGGCTGGG CATGGTGGGA
    CGGCTCACCA ACCGCACCTA CCGCAAGCGC ATCGACAGCT TCGTCAAGCG CCAGATCGAG
    GACATGGACG ACCACAGGCC CTTCTTCACC TACTGGCTTA CCTTCGTGCA CTCGCTCGTC
    ACCATCCTAG CCGTGTGCAT CTATGGCATC GCGCCCGTGG GCTTCTCGCA GCATGAGACG
    GTGGACTCGG TGCTGCGGAA CCGCGGGGTC TACGAGAACG TCAAGTACGT GCAGCAGGAG
    AACTTCTGGA TCGGGCCCAG CTCGTCCACG CTGGCAGTGT GGGTGAAGTG GCCCATCCAT
    CCCAGCGCCC CAGAGCTTGC GGGCCACAAG AGACAGTTTG GCTCTGTCTG CCACCAGGAT
    CCCAGGGTGT GTGATGAGCC CTCCTCCGAA GACCCTCATG AGTGGCCAGA AGACATCACC
    AAGTGGCCGA TCTGCACCAA AAACAGCGCT GGGAACCACA CCAACCATCC CCACATGGAC
    TGTGTCATCA CAGGACGGCC CTGCTGCATT GGCACCAAGG GCAGGTGTGA GATCACCTCC
    CGGGAGTACT GTGACTTCAT GAGGGGCTAC TTCCATGAGG AGGCCACGCT CTGCTCTCAG
    GTGCACTGCA TGGATGATGT GTGTGGGCTC CTGCCTTTTC TCAACCCCGA GGTGCCTGAC
    CAGTTCTACC GCCTGTGGCT ATCCCTCTTC CTGCACGCCG GGATCTTGCA CTGCCTGGTG
    TCCATCTGCT TCCAGATGAC TGTCCTGCGG GACCTGGAGA AGCTGGCAGG CTGGCACCGC
    ATAGCCATCA TCTACCTGCT GAGTGGTGTC ACCGGCAACC TGGCCAGTGC CATCTTCCTG
    CCATACCGAG CAGAGGTGGG TCCTGCTGGC TCCCAGTTCG GCATCCTGGC CTGCCTCTTC
    GTGGAGCTCT TCCAGAGCTG GCAGATCCTG GCGCGGCCCT GGCGTGCCTT CTTCAAGCTG
    CTGGCTGTGG TGCTCTTCCT CTTCACCTTT GGGCTGCTGC CGTGGATTGA CAACTTTGCC
    CACATCTCAG GGTTCATCAG TGGCCTCTTC CTCTCCTTCG CCTTCTTGCC CTACATCAGC
    TTTGGCAAGT TCGACCTGTA CCGGAAACGC TGCCAGATCA TCATCTTTCA GGTGGTCTTC
    CTGGGCCTCC TGGCTGGCCT GGTGGTCCTC TTCTACGTCT ATCCTGTCCG CTGTGAGTGG
    TGTGAGTTCC TCACCTGCAT CCCCTTCACT GACAAGTTCT GTGAGAAGTA CGAACTGGAC
    GCTCAGCTCC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016879046.1
    CDS25..2499
    Translation

    Target ORF information:

    RefSeq Version XM_017023557.1
    Organism Homo sapiens(human)
    Definition Homo sapiens rhomboid 5 homolog 1 (RHBDF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017023557.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGAGTGAGG CCCGCAGGGA CAGCACGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTA 
    AAGCTGGACA TTCCCTCTGC GGTGCCCCTG ACGGCAGAAG AGCCCAGCTT CCTGCAGGTA
    GGCCCTGGCC TGCAGGGACT GTGGGTGCCC CCTGTCCAGT ACCCTCACCA TGACCCTGTT
    GCCCAGCCCC TGAGGCGACA GGCTTTCCTG AGGAGTGTGA GTATGCCAGC CGAGACAGCC
    CACATCTCTT CACCCCACCA TGAGCTCCGG CGGCCGGTGC TGCAACGCCA GACGTCCATC
    ACACAGACCA TCCGCAGGGG GACCGCCGAC TGGTTTGGAG TGAGCAAGGA CAGTGACAGC
    ACCCAGAAAT GGCAGCGCAA GAGCATCCGT CACTGCAGCC AGCGCTACGG GAAGCTGAAG
    CCCCAGGTCC TCCGGGAGCT GGACCTGCCC AGCCAGGACA ACGTGTCGCT GACCAGCACC
    GAGACGCCAC CCCCACTCTA CGTGGGGCCA TGCCAGCTGG GCATGCAGAA GATCATAGAC
    CCCCTGGCCC GTGGCCGTGC CTTCCGTGTG GCAGATGACA CTGCGGAAGG CCTGAGTGCC
    CCACACACTC CCGTCACGCC GGGTGCTGCC TCCCTCTGCT CCTTCTCCAG CTCCCGCTCA
    GGTTTCCACC GGCTCCCGCG GCGGCGCAAG CGAGAGTCGG TGGCCAAGAT GAGCTTCCGG
    GCGGCCGCAG CGCTGATGAA AGGCCGCTCC GTTAGGGATG GCACCTTTCG CCGGGCACAG
    CGTCGAAGCT TCACTCCAGC TAGCTTTCTG GAGGAGGACA CAACTGATTT CCCCGATGAG
    CTGGACACAT CCTTCTTTGC CCGGGAAGGT ATCCTCCATG AAGAGCTGTC CACATACCCG
    GATGAAGTTT TCGAGTCCCC ATCGGAGGCA GCGCTAAAGG ACTGGGAGAA GGCACCGGAG
    CAGGCGGACC TCACCGGCGG GGCCCTGGAC CGCAGCGAGC TTGAGCGCAG CCACCTGATG
    CTGCCCTTGG AGCGAGGCTG GCGGAAGCAG AAGGAGGGCG CCGCAGCCCC GCAGCCCAAG
    GTGCGGCTCC GACAGGAGGT GGTGAGCACC GCGGGGCCGC GACGGGGCCA GCGTATCGCG
    GTGCCGGTGC GCAAGCTCTT CGCCCGGGAG AAGCGGCCGT ATGGGCTGGG CATGGTGGGA
    CGGCTCACCA ACCGCACCTA CCGCAAGCGC ATCGACAGCT TCGTCAAGCG CCAGATCGAG
    GACATGGACG ACCACAGGCC CTTCTTCACC TACTGGCTTA CCTTCGTGCA CTCGCTCGTC
    ACCATCCTAG CCGTGTGCAT CTATGGCATC GCGCCCGTGG GCTTCTCGCA GCATGAGACG
    GTGGACTCGG TGCTGCGGAA CCGCGGGGTC TACGAGAACG TCAAGTACGT GCAGCAGGAG
    AACTTCTGGA TCGGGCCCAG CTCGTCCACG CTGGCAGTGT GGGTGAAGTG GCCCATCCAT
    CCCAGCGCCC CAGAGCTTGC GGGCCACAAG AGACAGTTTG GCTCTGTCTG CCACCAGGAT
    CCCAGGGTGT GTGATGAGCC CTCCTCCGAA GACCCTCATG AGTGGCCAGA AGACATCACC
    AAGTGGCCGA TCTGCACCAA AAACAGCGCT GGGAACCACA CCAACCATCC CCACATGGAC
    TGTGTCATCA CAGGACGGCC CTGCTGCATT GGCACCAAGG GCAGGTGTGA GATCACCTCC
    CGGGAGTACT GTGACTTCAT GAGGGGCTAC TTCCATGAGG AGGCCACGCT CTGCTCTCAG
    GTGCACTGCA TGGATGATGT GTGTGGGCTC CTGCCTTTTC TCAACCCCGA GGTGCCTGAC
    CAGTTCTACC GCCTGTGGCT ATCCCTCTTC CTGCACGCCG GGATCTTGCA CTGCCTGGTG
    TCCATCTGCT TCCAGATGAC TGTCCTGCGG GACCTGGAGA AGCTGGCAGG CTGGCACCGC
    ATAGCCATCA TCTACCTGCT GAGTGGTGTC ACCGGCAACC TGGCCAGTGC CATCTTCCTG
    CCATACCGAG CAGAGGTGGG TCCTGCTGGC TCCCAGTTCG GCATCCTGGC CTGCCTCTTC
    GTGGAGCTCT TCCAGAGCTG GCAGATCCTG GCGCGGCCCT GGCGTGCCTT CTTCAAGCTG
    CTGGCTGTGG TGCTCTTCCT CTTCACCTTT GGGCTGCTGC CGTGGATTGA CAACTTTGCC
    CACATCTCAG GGTTCATCAG TGGCCTCTTC CTCTCCTTCG CCTTCTTGCC CTACATCAGC
    TTTGGCAAGT TCGACCTGTA CCGGAAACGC TGCCAGATCA TCATCTTTCA GGTGGTCTTC
    CTGGGCCTCC TGGCTGGCCT GGTGGTCCTC TTCTACGTCT ATCCTGTCCG CTGTGAGTGG
    TGTGAGTTCC TCACCTGCAT CCCCTTCACT GACAAGTTCT GTGAGAAGTA CGAACTGGAC
    GCTCAGCTCC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu86214
    Clone ID Related Accession (Same CDS sequence) XM_017023558.1
    Accession Version XM_017023558.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1848bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product inactive rhomboid protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAGTGAGG CCCGCAGGGA CAGCACGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTA 
    AAGCTGGACA TTCCCTCTGC GGTGCCCCTG ACGGCAGAAG AGCCCAGCTT CCTGCAGGTA
    GGCCCTGGCC TGCAGGGACT GTGGGTGCCC CCTGTCCAGT ACCCTCACCA TGACCCTGTT
    GCCCAGCCCC TGAGGCGACA GGCTTTCCTG AGGAGTGTGA GTATGCCAGC CGAGACAGCC
    CACATCTCTT CACCCCACCA TGAGCTCCGG CGGCCGGTGC TGCAACGCCA GACGTCCATC
    ACACAGACCA TCCGCAGGGG GACCGCCGAC TGGTTTGGAG TGAGCAAGGA CAGTGACAGC
    ACCCAGAAAT GGCAGCGCAA GAGCATCCGT CACTGCAGCC AGCGCTACGG GAAGCTGAAG
    CCCCAGGTCC TCCGGGAGCT GGACCTGCCC AGCCAGGACA ACGTGTCGCT GACCAGCACC
    GAGACGCCAC CCCCACTCTA CGTGGGGCCA TGCCAGCTGG GCATGCAGAA GATCATAGAC
    CCCCTGGCCC GTGGCCGTGC CTTCCGTGTG GCAGATGACA CTGCGGAAGG CCTGAGTGCC
    CCACACACTC CCGTCACGCC GGGTGCTGCC TCCCTCTGCT CCTTCTCCAG CTCCCGCTCA
    GGTTTCCACC GGCTCCCGCG GCGGCGCAAG CGAGAGTCGG TGGCCAAGAT GAGCTTCCGG
    GCGGCCGCAG CGCTGATGAA AGGCCGCTCC GTTAGGGATG GCACCTTTCG CCGGGCACAG
    CGTCGAAGCT TCACTCCAGC TAGCTTTCTG GAGGAGGACA CAACTGATTT CCCCGATGAG
    CTGGACACAT CCTTCTTTGC CCGGGAAGGT ATCCTCCATG AAGAGCTGTC CACATACCCG
    GATGAAGTTT TCGAGTCCCC ATCGGAGGCA GCGCTAAAGG ACTGGGAGAA GGCACCGGAG
    CAGGCGGACC TCACCGGCGG GGCCCTGGAC CGCAGCGAGC TTGAGCGCAG CCACCTGATG
    CTGCCCTTGG AGCGAGGCTG GCGGAAGCAG AAGGAGGGCG CCGCAGCCCC GCAGCCCAAG
    GTGCGGCTCC GACAGGAGGT GGTGAGCACC GCGGGGCCGC GACGGGGCCA GCGTATCGCG
    GTGCCGGTGC GCAAGCTCTT CGCCCGGGAG AAGCGGCCGT ATGGGCTGGG CATGGTGGGA
    CGGCTCACCA ACCGCACCTA CCGCAAGCGC ATCGACAGCT TCGTCAAGCG CCAGATCGAG
    GACATGGACG ACCACAGGCC CTTCTTCACC TACTGGCTTA CCTTCGTGCA CTCGCTCGTC
    ACCATCCTAG CCGTGTGCAT CTATGGCATC GCGCCCGTGG GCTTCTCGCA GCATGAGACG
    GTGGACTCGG TGCTGCGGAA CCGCGGGGTC TACGAGAACG TCAAGTACGT GCAGCAGGAG
    AACTTCTGGA TCGGGCCCAG CTCGGAGGCC CTCATCCACC TGGGCGCCAA GTTTTCGCCC
    TGCATGCGCC AGGACCCGCA GGTGCACAGC TTCATTCGCT CGGCGCGCGA GCGCGAGAAG
    CACTCCGCCT GCTGCGTGCG CAACGACAGG TCGGGCTGCG TGCAGACCTC GGAGGAGGAG
    TGCTCGTCCA CGCTGGCAGT GTGGGTGAAG TGGCCCATCC ATCCCAGCGC CCCAGAGCTT
    GCGGGCCACA AGAGACAGTT TGGCTCTGTC TGCCACCAGG ATCCCAGGGT GTGTGATGAG
    CCCTCCTCCG AAGACCCTCA TGAGTGGCCA GAAGACATCA CCAAGTGGCC GAGGCTGTGC
    ACCTGCCCTT TGTACAGCCT GTCCATCAAC CAGCGCCTGC CCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016879047.1
    CDS25..1872
    Translation

    Target ORF information:

    RefSeq Version XM_017023558.1
    Organism Homo sapiens(human)
    Definition Homo sapiens rhomboid 5 homolog 1 (RHBDF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017023558.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAGTGAGG CCCGCAGGGA CAGCACGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTA 
    AAGCTGGACA TTCCCTCTGC GGTGCCCCTG ACGGCAGAAG AGCCCAGCTT CCTGCAGGTA
    GGCCCTGGCC TGCAGGGACT GTGGGTGCCC CCTGTCCAGT ACCCTCACCA TGACCCTGTT
    GCCCAGCCCC TGAGGCGACA GGCTTTCCTG AGGAGTGTGA GTATGCCAGC CGAGACAGCC
    CACATCTCTT CACCCCACCA TGAGCTCCGG CGGCCGGTGC TGCAACGCCA GACGTCCATC
    ACACAGACCA TCCGCAGGGG GACCGCCGAC TGGTTTGGAG TGAGCAAGGA CAGTGACAGC
    ACCCAGAAAT GGCAGCGCAA GAGCATCCGT CACTGCAGCC AGCGCTACGG GAAGCTGAAG
    CCCCAGGTCC TCCGGGAGCT GGACCTGCCC AGCCAGGACA ACGTGTCGCT GACCAGCACC
    GAGACGCCAC CCCCACTCTA CGTGGGGCCA TGCCAGCTGG GCATGCAGAA GATCATAGAC
    CCCCTGGCCC GTGGCCGTGC CTTCCGTGTG GCAGATGACA CTGCGGAAGG CCTGAGTGCC
    CCACACACTC CCGTCACGCC GGGTGCTGCC TCCCTCTGCT CCTTCTCCAG CTCCCGCTCA
    GGTTTCCACC GGCTCCCGCG GCGGCGCAAG CGAGAGTCGG TGGCCAAGAT GAGCTTCCGG
    GCGGCCGCAG CGCTGATGAA AGGCCGCTCC GTTAGGGATG GCACCTTTCG CCGGGCACAG
    CGTCGAAGCT TCACTCCAGC TAGCTTTCTG GAGGAGGACA CAACTGATTT CCCCGATGAG
    CTGGACACAT CCTTCTTTGC CCGGGAAGGT ATCCTCCATG AAGAGCTGTC CACATACCCG
    GATGAAGTTT TCGAGTCCCC ATCGGAGGCA GCGCTAAAGG ACTGGGAGAA GGCACCGGAG
    CAGGCGGACC TCACCGGCGG GGCCCTGGAC CGCAGCGAGC TTGAGCGCAG CCACCTGATG
    CTGCCCTTGG AGCGAGGCTG GCGGAAGCAG AAGGAGGGCG CCGCAGCCCC GCAGCCCAAG
    GTGCGGCTCC GACAGGAGGT GGTGAGCACC GCGGGGCCGC GACGGGGCCA GCGTATCGCG
    GTGCCGGTGC GCAAGCTCTT CGCCCGGGAG AAGCGGCCGT ATGGGCTGGG CATGGTGGGA
    CGGCTCACCA ACCGCACCTA CCGCAAGCGC ATCGACAGCT TCGTCAAGCG CCAGATCGAG
    GACATGGACG ACCACAGGCC CTTCTTCACC TACTGGCTTA CCTTCGTGCA CTCGCTCGTC
    ACCATCCTAG CCGTGTGCAT CTATGGCATC GCGCCCGTGG GCTTCTCGCA GCATGAGACG
    GTGGACTCGG TGCTGCGGAA CCGCGGGGTC TACGAGAACG TCAAGTACGT GCAGCAGGAG
    AACTTCTGGA TCGGGCCCAG CTCGGAGGCC CTCATCCACC TGGGCGCCAA GTTTTCGCCC
    TGCATGCGCC AGGACCCGCA GGTGCACAGC TTCATTCGCT CGGCGCGCGA GCGCGAGAAG
    CACTCCGCCT GCTGCGTGCG CAACGACAGG TCGGGCTGCG TGCAGACCTC GGAGGAGGAG
    TGCTCGTCCA CGCTGGCAGT GTGGGTGAAG TGGCCCATCC ATCCCAGCGC CCCAGAGCTT
    GCGGGCCACA AGAGACAGTT TGGCTCTGTC TGCCACCAGG ATCCCAGGGT GTGTGATGAG
    CCCTCCTCCG AAGACCCTCA TGAGTGGCCA GAAGACATCA CCAAGTGGCC GAGGCTGTGC
    ACCTGCCCTT TGTACAGCCT GTCCATCAAC CAGCGCCTGC CCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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