×

SGCA cDNA ORF clone, Homo sapiens (human)

Gene Symbol SGCA
Entrez Gene ID 6442
Full Name sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein)
Synonyms 50-DAG, A2, ADL, DAG2, DMDA2, LGMD2D, SCARMD1, adhalin
General protein information
Preferred Names
alpha-sarcoglycan
Names
alpha-sarcoglycan
50DAG
50kD DAG
alpha-SG
dystroglycan-2
50 kDa dystrophin-associated glycoprotein
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

17

17q21

Summary This gene encodes a component of the dystrophin-glycoprotein complex (DGC), which is critical to the stability of muscle fiber membranes and to the linking of the actin cytoskeleton to the extracellular matrix. Its expression is thought to be restricted to striated muscle. Mutations in this gene result in type 2D autosomal recessive limb-girdle muscular dystrophy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]. lac of sum
Disorder MIM:

600119

Disorder Html: Muscular dystrophy, limb-girdle, type 2D, 608099 (3); Adhalinopathy,

mRNA and Protein(s)

mRNA Protein Name
XM_011525120 XP_011523422 alpha-sarcoglycan isoform X1
XM_011525121 XP_011523423 alpha-sarcoglycan isoform X2
XM_011525122 XP_011523424 alpha-sarcoglycan isoform X3
XM_011525123 XP_011523425 alpha-sarcoglycan isoform X4
XM_011525124 XP_011523426 alpha-sarcoglycan isoform X5
NM_000023 NP_000014 alpha-sarcoglycan isoform 1 precursor
NM_001135697 NP_001129169 alpha-sarcoglycan isoform 2 precursor



Homo sapiens (human) SGCA NP_000014.1
Pan troglodytes (chimpanzee) SGCA XP_523792.2
Macaca mulatta (Rhesus monkey) SGCA XP_001092850.1
Canis lupus familiaris (dog) SGCA XP_005624692.1
Bos taurus (cattle) SGCA NP_001013420.1
Mus musculus (house mouse) Sgca NP_033187.1
Rattus norvegicus (Norway rat) Sgca NP_001100509.1
Xenopus (Silurana) tropicalis (western clawed frog) sgca XP_002935590.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SGCA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SGCA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Free ORF Clone
for Every Scientist
More Detail >>

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu60457 XM_011525120 PREDICTED: Homo sapiens sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein) (SGCA), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu60458 XM_011525121 PREDICTED: Homo sapiens sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein) (SGCA), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu60459 XM_011525122 PREDICTED: Homo sapiens sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein) (SGCA), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu60460 XM_011525123 PREDICTED: Homo sapiens sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein) (SGCA), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu60461 XM_011525124 PREDICTED: Homo sapiens sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein) (SGCA), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu19381
NM_000023 Homo sapiens sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein) (SGCA), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu31407 NM_001135697 Homo sapiens sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein) (SGCA), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $188.30
$269.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu60457
Clone ID Related Accession (Same CDS sequence) XM_011525120
Accession Version XM_011525120.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1242bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product alpha-sarcoglycan isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGCTGAGA CACTCTTCTG GACTCCTCTC CTCGTGGTTC TCCTGGCAGG GCTGGGGGAC 
ACCGAGGCCC AGCAGACCAC GCTACACCCA CTTGTGGGCC GTGTCTTTGT GCACACCTTG
GACCATGAGA CGTTTCTGAG CCTTCCTGAG CATGTCGCTG TCCCACCCGC TGTCCACATC
ACCTACCACG CCCACCTCCA GGGACACCCA GACCTGCCCC GGTGGCTCCG CTACACCCAG
CGCAGCCCCC ACCACCCTGG CTTCCTCTAC GGCTCTGCCA CCCCAGAAGA TCGTGGGCTC
CAGGTCATTG AGGTCACAGC CTACAATCGG GACAGCTTTG ATACCACTCG GCAGAGGCTG
GTGCTGGAGA TTGGGGACCC AGAAGGCCCC CTGCTGCCAT ACCAAGCCGA GTTCCTGGTG
CGCAGCCACG ATGCGGAGGA GGTGCTGCCC TCAACACCTG CCAGCCGCTT CCTCTCAGCC
TTGGGGGGAC TCTGGGAGCC CGGAGAGCTT CAGCTGCTCA ACGTCACCTC TGCCTTGGAC
CGTGGGGGCC GTGTCCCCCT TCCCATTGAG GGCCGAAAAG AAGGGGTATA CATTAAGGTG
GGTTCTGCCT CACCTTTTTC TACTTGCCTG AAGATGGTGG CATCCCCCGA TAGCCACGCC
CGCTGTGCCC AGGGCCAGCC TCCACTTCTG TCTTGCTACG ACACCTTGGC ACCCCACTTC
CGCGTTGACT GGTGCAATGT GACCCTGGTG GATAAGTCAG TGCCGGAGCC TGCAGATGAG
GTGCCCACCC CAGGTGATGG GATCCTGGAG CATGACCCGT TCTTCTGCCC ACCCACTGAG
GCCCCAGACC GTGACTTCTT GGTGGATGCT CTGGTCACCC TCCTGGTGCC CCTGCTGGTG
GCCCTGCTTC TCACCTTGCT GCTGGCCTAT GTCATGTGCT GCCGGCGGGA GGGAAGGCTG
AAGAGAGACC TGGCTACCTC CGACATCCAG ATGGTCCACC ACTGCACCAT CCACGGGAAC
ACAGAGGAGC TGCGGCAGAT GGCGGCCAGC CGCGAGGTGC CCCGGCCACT CTCCACCCTG
CCCATGTTCA ATGTGCACAC AGTGGTTCCA GGTCCAGCCC TGACTTCATC CTCCCTTCTC
TGTCCACACC ACGAGTGGCA CATCCCACCT GCTGATTCCA GCTCCTGGCC CTCCTGGAAC
CCAGGCTCTA AACAAGCAGG GAGAGGGGGT GGGGTGGGGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011523422.1
CDS67..1308
Misc Feature(1)127..459(+)
Misc Feature(2)145..1167(+)
Misc Feature(3)160..453(+)
Translation

Target ORF information:

RefSeq Version XM_011525120
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein) (SGCA), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011525120

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGCTGAGA CACTCTTCTG GACTCCTCTC CTCGTGGTTC TCCTGGCAGG GCTGGGGGAC 
ACCGAGGCCC AGCAGACCAC GCTACACCCA CTTGTGGGCC GTGTCTTTGT GCACACCTTG
GACCATGAGA CGTTTCTGAG CCTTCCTGAG CATGTCGCTG TCCCACCCGC TGTCCACATC
ACCTACCACG CCCACCTCCA GGGACACCCA GACCTGCCCC GGTGGCTCCG CTACACCCAG
CGCAGCCCCC ACCACCCTGG CTTCCTCTAC GGCTCTGCCA CCCCAGAAGA TCGTGGGCTC
CAGGTCATTG AGGTCACAGC CTACAATCGG GACAGCTTTG ATACCACTCG GCAGAGGCTG
GTGCTGGAGA TTGGGGACCC AGAAGGCCCC CTGCTGCCAT ACCAAGCCGA GTTCCTGGTG
CGCAGCCACG ATGCGGAGGA GGTGCTGCCC TCAACACCTG CCAGCCGCTT CCTCTCAGCC
TTGGGGGGAC TCTGGGAGCC CGGAGAGCTT CAGCTGCTCA ACGTCACCTC TGCCTTGGAC
CGTGGGGGCC GTGTCCCCCT TCCCATTGAG GGCCGAAAAG AAGGGGTATA CATTAAGGTG
GGTTCTGCCT CACCTTTTTC TACTTGCCTG AAGATGGTGG CATCCCCCGA TAGCCACGCC
CGCTGTGCCC AGGGCCAGCC TCCACTTCTG TCTTGCTACG ACACCTTGGC ACCCCACTTC
CGCGTTGACT GGTGCAATGT GACCCTGGTG GATAAGTCAG TGCCGGAGCC TGCAGATGAG
GTGCCCACCC CAGGTGATGG GATCCTGGAG CATGACCCGT TCTTCTGCCC ACCCACTGAG
GCCCCAGACC GTGACTTCTT GGTGGATGCT CTGGTCACCC TCCTGGTGCC CCTGCTGGTG
GCCCTGCTTC TCACCTTGCT GCTGGCCTAT GTCATGTGCT GCCGGCGGGA GGGAAGGCTG
AAGAGAGACC TGGCTACCTC CGACATCCAG ATGGTCCACC ACTGCACCAT CCACGGGAAC
ACAGAGGAGC TGCGGCAGAT GGCGGCCAGC CGCGAGGTGC CCCGGCCACT CTCCACCCTG
CCCATGTTCA ATGTGCACAC AGTGGTTCCA GGTCCAGCCC TGACTTCATC CTCCCTTCTC
TGTCCACACC ACGAGTGGCA CATCCCACCT GCTGATTCCA GCTCCTGGCC CTCCTGGAAC
CCAGGCTCTA AACAAGCAGG GAGAGGGGGT GGGGTGGGGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60458
Clone ID Related Accession (Same CDS sequence) XM_011525121
Accession Version XM_011525121.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1092bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product alpha-sarcoglycan isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCTGAGA CACTCTTCTG GACTCCTCTC CTCGTGGTTC TCCTGGCAGG GCTGGGGGAC 
ACCGAGGCCC AGCAGACCAC GCTACACCCA CTTGTGGGCC GTGTCTTTGT GCACACCTTG
GACCATGAGA CGTTTCTGAG CCTTCCTGAG CATGTCGCTG TCCCACCCGC TGTCCACATC
ACCTACCACG CCCACCTCCA GGGACACCCA GACCTGCCCC GGTGGCTCCG CTACACCCAG
CGCAGCCCCC ACCACCCTGG CTTCCTCTAC GGCTCTGCCA CCCCAGAAGA TCGTGGGCTC
CAGGTCATTG AGGTCACAGC CTACAATCGG GACAGCTTTG ATACCACTCG GCAGAGGCTG
GTGCTGGAGA TTGGGGACCC AGAAGGCCCC CTGCTGCCAT ACCAAGCCGA GTTCCTGGTG
CGCAGCCACG ATGCGGAGGA GGTGCTGCCC TCAACACCTG CCAGCCGCTT CCTCTCAGCC
TTGGGGGGAC TCTGGGAGCC CGGAGAGCTT CAGCTGCTCA ACGTCACCTC TGCCTTGGAC
CGTGGGGGCC GTGTCCCCCT TCCCATTGAG GGCCGAAAAG AAGGGGTATA CATTAAGGTG
GATAAGTCAG TGCCGGAGCC TGCAGATGAG GTGCCCACCC CAGGTGATGG GATCCTGGAG
CATGACCCGT TCTTCTGCCC ACCCACTGAG GCCCCAGACC GTGACTTCTT GGTGGATGCT
CTGGTCACCC TCCTGGTGCC CCTGCTGGTG GCCCTGCTTC TCACCTTGCT GCTGGCCTAT
GTCATGTGCT GCCGGCGGGA GGGAAGGCTG AAGAGAGACC TGGCTACCTC CGACATCCAG
ATGGTCCACC ACTGCACCAT CCACGGGAAC ACAGAGGAGC TGCGGCAGAT GGCGGCCAGC
CGCGAGGTGC CCCGGCCACT CTCCACCCTG CCCATGTTCA ATGTGCACAC AGTGGTTCCA
GGTCCAGCCC TGACTTCATC CTCCCTTCTC TGTCCACACC ACGAGTGGCA CATCCCACCT
GCTGATTCCA GCTCCTGGCC CTCCTGGAAC CCAGGCTCTA AACAAGCAGG GAGAGGGGGT
GGGGTGGGGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011523423.1
CDS67..1158
Misc Feature(1)127..459(+)
Misc Feature(2)145..1017(+)
Misc Feature(3)160..453(+)
Translation

Target ORF information:

RefSeq Version XM_011525121
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein) (SGCA), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011525121

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCTGAGA CACTCTTCTG GACTCCTCTC CTCGTGGTTC TCCTGGCAGG GCTGGGGGAC 
ACCGAGGCCC AGCAGACCAC GCTACACCCA CTTGTGGGCC GTGTCTTTGT GCACACCTTG
GACCATGAGA CGTTTCTGAG CCTTCCTGAG CATGTCGCTG TCCCACCCGC TGTCCACATC
ACCTACCACG CCCACCTCCA GGGACACCCA GACCTGCCCC GGTGGCTCCG CTACACCCAG
CGCAGCCCCC ACCACCCTGG CTTCCTCTAC GGCTCTGCCA CCCCAGAAGA TCGTGGGCTC
CAGGTCATTG AGGTCACAGC CTACAATCGG GACAGCTTTG ATACCACTCG GCAGAGGCTG
GTGCTGGAGA TTGGGGACCC AGAAGGCCCC CTGCTGCCAT ACCAAGCCGA GTTCCTGGTG
CGCAGCCACG ATGCGGAGGA GGTGCTGCCC TCAACACCTG CCAGCCGCTT CCTCTCAGCC
TTGGGGGGAC TCTGGGAGCC CGGAGAGCTT CAGCTGCTCA ACGTCACCTC TGCCTTGGAC
CGTGGGGGCC GTGTCCCCCT TCCCATTGAG GGCCGAAAAG AAGGGGTATA CATTAAGGTG
GATAAGTCAG TGCCGGAGCC TGCAGATGAG GTGCCCACCC CAGGTGATGG GATCCTGGAG
CATGACCCGT TCTTCTGCCC ACCCACTGAG GCCCCAGACC GTGACTTCTT GGTGGATGCT
CTGGTCACCC TCCTGGTGCC CCTGCTGGTG GCCCTGCTTC TCACCTTGCT GCTGGCCTAT
GTCATGTGCT GCCGGCGGGA GGGAAGGCTG AAGAGAGACC TGGCTACCTC CGACATCCAG
ATGGTCCACC ACTGCACCAT CCACGGGAAC ACAGAGGAGC TGCGGCAGAT GGCGGCCAGC
CGCGAGGTGC CCCGGCCACT CTCCACCCTG CCCATGTTCA ATGTGCACAC AGTGGTTCCA
GGTCCAGCCC TGACTTCATC CTCCCTTCTC TGTCCACACC ACGAGTGGCA CATCCCACCT
GCTGATTCCA GCTCCTGGCC CTCCTGGAAC CCAGGCTCTA AACAAGCAGG GAGAGGGGGT
GGGGTGGGGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60459
Clone ID Related Accession (Same CDS sequence) XM_011525122
Accession Version XM_011525122.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 966bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product alpha-sarcoglycan isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTGAGA CACTCTTCTG GACTCCTCTC CTCGTGGTTC TCCTGGCAGG GCTGGGGGAC 
ACCGAGGCCC AGCAGACCAC GCTACACCCA CTTGTGGGCC GTGTCTTTGT GCACACCTTG
GACCATGAGA CGTTTCTGAG CCTTCCTGAG CATGTCGCTG TCCCACCCGC TGTCCACATC
ACCTACCACG CCCACCTCCA GGGACACCCA GACCTGCCCC GGTGGCTCCG CTACACCCAG
CGCAGCCCCC ACCACCCTGG CTTCCTCTAC GGCTCTGCCA CCCCAGAAGA TCGTGGGCTC
CAGGTCATTG AGGTCACAGC CTACAATCGG GACAGCTTTG ATACCACTCG GCAGAGGCTG
GTGCTGGAGA TTGGGGACCC AGAAGGCCCC CTGCTGCCAT ACCAAGCCGA GTTCCTGGTG
CGCAGCCACG ATGCGGAGGA GGTGCTGCCC TCAACACCTG CCAGCCGCTT CCTCTCAGCC
TTGGGGGGAC TCTGGGAGCC CGGAGAGCTT CAGCTGCTCA ACGTCACCTC TGCCTTGGAC
CGTGGGGGCC GTGTCCCCCT TCCCATTGAG GGCCGAAAAG AAGGGGTATA CATTAAGGTG
GGTTCTGCCT CACCTTTTTC TACTTGCCTG AAGATGGTGG CATCCCCCGA TAGCCACGCC
CGCTGTGCCC AGGGCCAGCC TCCACTTCTG TCTTGCTACG ACACCTTGGC ACCCCACTTC
CGCGTTGACT GGTGCAATGT GACCCTGGCT GAAGAGAGAC CTGGCTACCT CCGACATCCA
GATGGTCCAC CACTGCACCA TCCACGGGAA CACAGAGGAG CTGCGGCAGA TGGCGGCCAG
CCGCGAGGTG CCCCGGCCAC TCTCCACCCT GCCCATGTTC AATGTGCACA CAGTGGTTCC
AGGTCCAGCC CTGACTTCAT CCTCCCTTCT CTGTCCACAC CACGAGTGGC ACATCCCACC
TGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011523424.1
CDS67..1032
Misc Feature(1)127..459(+)
Misc Feature(2)145..>876(+)
Misc Feature(3)160..453(+)
Translation

Target ORF information:

RefSeq Version XM_011525122
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein) (SGCA), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011525122

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTGAGA CACTCTTCTG GACTCCTCTC CTCGTGGTTC TCCTGGCAGG GCTGGGGGAC 
ACCGAGGCCC AGCAGACCAC GCTACACCCA CTTGTGGGCC GTGTCTTTGT GCACACCTTG
GACCATGAGA CGTTTCTGAG CCTTCCTGAG CATGTCGCTG TCCCACCCGC TGTCCACATC
ACCTACCACG CCCACCTCCA GGGACACCCA GACCTGCCCC GGTGGCTCCG CTACACCCAG
CGCAGCCCCC ACCACCCTGG CTTCCTCTAC GGCTCTGCCA CCCCAGAAGA TCGTGGGCTC
CAGGTCATTG AGGTCACAGC CTACAATCGG GACAGCTTTG ATACCACTCG GCAGAGGCTG
GTGCTGGAGA TTGGGGACCC AGAAGGCCCC CTGCTGCCAT ACCAAGCCGA GTTCCTGGTG
CGCAGCCACG ATGCGGAGGA GGTGCTGCCC TCAACACCTG CCAGCCGCTT CCTCTCAGCC
TTGGGGGGAC TCTGGGAGCC CGGAGAGCTT CAGCTGCTCA ACGTCACCTC TGCCTTGGAC
CGTGGGGGCC GTGTCCCCCT TCCCATTGAG GGCCGAAAAG AAGGGGTATA CATTAAGGTG
GGTTCTGCCT CACCTTTTTC TACTTGCCTG AAGATGGTGG CATCCCCCGA TAGCCACGCC
CGCTGTGCCC AGGGCCAGCC TCCACTTCTG TCTTGCTACG ACACCTTGGC ACCCCACTTC
CGCGTTGACT GGTGCAATGT GACCCTGGCT GAAGAGAGAC CTGGCTACCT CCGACATCCA
GATGGTCCAC CACTGCACCA TCCACGGGAA CACAGAGGAG CTGCGGCAGA TGGCGGCCAG
CCGCGAGGTG CCCCGGCCAC TCTCCACCCT GCCCATGTTC AATGTGCACA CAGTGGTTCC
AGGTCCAGCC CTGACTTCAT CCTCCCTTCT CTGTCCACAC CACGAGTGGC ACATCCCACC
TGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60460
Clone ID Related Accession (Same CDS sequence) XM_011525123
Accession Version XM_011525123.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 870bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product alpha-sarcoglycan isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCTGAGA CACTCTTCTG GACTCCTCTC CTCGTGGTTC TCCTGGCAGG GCTGGGGGAC 
ACCGAGGCCC AGCAGACCAC GCTACACCCA CTTGTGGGCC GTGTCTTTGT GCACACCTTG
GACCATGAGA CGTTTCTGAG CCTTCCTGAG CATGTCGCTG TCCCACCCGC TGTCCACATC
ACCTACCACG CCCACCTCCA GGGACACCCA GACCTGCCCC GGTGGCTCCG CTACACCCAG
CGCAGCCCCC ACCACCCTGG CTTCCTCTAC GGCTCTGCCA CCCCAGAAGA TCGTGGGCTC
CAGGTCATTG AGGTCACAGC CTACAATCGG GACAGCTTTG ATACCACTCG GCAGAGGCTG
GTGCTGGAGA TTGGGGACCC AGAAGGCCCC CTGCTGCCAT ACCAAGCCGA GTTCCTGGTG
CGCAGCCACG ATGCGGAGGA GGTGCTGCCC TCAACACCTG CCAGCCGCTT CCTCTCAGCC
TTGGGGGGAC TCTGGGAGCC CGGAGAGCTT CAGCTGCTCA ACGTCACCTC TGCCTTGGAC
CGTGGGGGCC GTGTCCCCCT TCCCATTGAG GGCCGAAAAG AAGGGCTGAA GAGAGACCTG
GCTACCTCCG ACATCCAGAT GGTCCACCAC TGCACCATCC ACGGGAACAC AGAGGAGCTG
CGGCAGATGG CGGCCAGCCG CGAGGTGCCC CGGCCACTCT CCACCCTGCC CATGTTCAAT
GTGCACACAG TGGTTCCAGG TCCAGCCCTG ACTTCATCCT CCCTTCTCTG TCCACACCAC
GAGTGGCACA TCCCACCTGC TGATTCCAGC TCCTGGCCCT CCTGGAACCC AGGCTCTAAA
CAAGCAGGGA GAGGGGGTGG GGTGGGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011523425.1
CDS67..936
Misc Feature(1)127..459(+)
Misc Feature(2)145..795(+)
Misc Feature(3)160..453(+)
Translation

Target ORF information:

RefSeq Version XM_011525123
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein) (SGCA), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011525123

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCTGAGA CACTCTTCTG GACTCCTCTC CTCGTGGTTC TCCTGGCAGG GCTGGGGGAC 
ACCGAGGCCC AGCAGACCAC GCTACACCCA CTTGTGGGCC GTGTCTTTGT GCACACCTTG
GACCATGAGA CGTTTCTGAG CCTTCCTGAG CATGTCGCTG TCCCACCCGC TGTCCACATC
ACCTACCACG CCCACCTCCA GGGACACCCA GACCTGCCCC GGTGGCTCCG CTACACCCAG
CGCAGCCCCC ACCACCCTGG CTTCCTCTAC GGCTCTGCCA CCCCAGAAGA TCGTGGGCTC
CAGGTCATTG AGGTCACAGC CTACAATCGG GACAGCTTTG ATACCACTCG GCAGAGGCTG
GTGCTGGAGA TTGGGGACCC AGAAGGCCCC CTGCTGCCAT ACCAAGCCGA GTTCCTGGTG
CGCAGCCACG ATGCGGAGGA GGTGCTGCCC TCAACACCTG CCAGCCGCTT CCTCTCAGCC
TTGGGGGGAC TCTGGGAGCC CGGAGAGCTT CAGCTGCTCA ACGTCACCTC TGCCTTGGAC
CGTGGGGGCC GTGTCCCCCT TCCCATTGAG GGCCGAAAAG AAGGGCTGAA GAGAGACCTG
GCTACCTCCG ACATCCAGAT GGTCCACCAC TGCACCATCC ACGGGAACAC AGAGGAGCTG
CGGCAGATGG CGGCCAGCCG CGAGGTGCCC CGGCCACTCT CCACCCTGCC CATGTTCAAT
GTGCACACAG TGGTTCCAGG TCCAGCCCTG ACTTCATCCT CCCTTCTCTG TCCACACCAC
GAGTGGCACA TCCCACCTGC TGATTCCAGC TCCTGGCCCT CCTGGAACCC AGGCTCTAAA
CAAGCAGGGA GAGGGGGTGG GGTGGGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60461
Clone ID Related Accession (Same CDS sequence) XM_011525124
Accession Version XM_011525124.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 936bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product alpha-sarcoglycan isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTCGGTCA CAGCCTACAA TCGGGACAGC TTTGATACCA CTCGGCAGAG GCTGGTGCTG 
GAGATTGGGG ACCCAGAAGG CCCCCTGCTG CCATACCAAG CCGAGTTCCT GGTGCGCAGC
CACGATGCGG AGGAGGTGCT GCCCTCAACA CCTGCCAGCC GCTTCCTCTC AGCCTTGGGG
GGACTCTGGG AGCCCGGAGA GCTTCAGCTG CTCAACGTCA CCTCTGCCTT GGACCGTGGG
GGCCGTGTCC CCCTTCCCAT TGAGGGCCGA AAAGAAGGGG TATACATTAA GGTGGGTTCT
GCCTCACCTT TTTCTACTTG CCTGAAGATG GTGGCATCCC CCGATAGCCA CGCCCGCTGT
GCCCAGGGCC AGCCTCCACT TCTGTCTTGC TACGACACCT TGGCACCCCA CTTCCGCGTT
GACTGGTGCA ATGTGACCCT GGTGGATAAG TCAGTGCCGG AGCCTGCAGA TGAGGTGCCC
ACCCCAGGTG ATGGGATCCT GGAGCATGAC CCGTTCTTCT GCCCACCCAC TGAGGCCCCA
GACCGTGACT TCTTGGTGGA TGCTCTGGTC ACCCTCCTGG TGCCCCTGCT GGTGGCCCTG
CTTCTCACCT TGCTGCTGGC CTATGTCATG TGCTGCCGGC GGGAGGGAAG GCTGAAGAGA
GACCTGGCTA CCTCCGACAT CCAGATGGTC CACCACTGCA CCATCCACGG GAACACAGAG
GAGCTGCGGC AGATGGCGGC CAGCCGCGAG GTGCCCCGGC CACTCTCCAC CCTGCCCATG
TTCAATGTGC ACACAGTGGT TCCAGGTCCA GCCCTGACTT CATCCTCCCT TCTCTGTCCA
CACCACGAGT GGCACATCCC ACCTGCTGAT TCCAGCTCCT GGCCCTCCTG GAACCCAGGC
TCTAAACAAG CAGGGAGAGG GGGTGGGGTG GGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011523426.1
CDS218..1153
Misc Feature(1)224..1012(+)
Translation

Target ORF information:

RefSeq Version XM_011525124
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein) (SGCA), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011525124

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTCGGTCA CAGCCTACAA TCGGGACAGC TTTGATACCA CTCGGCAGAG GCTGGTGCTG 
GAGATTGGGG ACCCAGAAGG CCCCCTGCTG CCATACCAAG CCGAGTTCCT GGTGCGCAGC
CACGATGCGG AGGAGGTGCT GCCCTCAACA CCTGCCAGCC GCTTCCTCTC AGCCTTGGGG
GGACTCTGGG AGCCCGGAGA GCTTCAGCTG CTCAACGTCA CCTCTGCCTT GGACCGTGGG
GGCCGTGTCC CCCTTCCCAT TGAGGGCCGA AAAGAAGGGG TATACATTAA GGTGGGTTCT
GCCTCACCTT TTTCTACTTG CCTGAAGATG GTGGCATCCC CCGATAGCCA CGCCCGCTGT
GCCCAGGGCC AGCCTCCACT TCTGTCTTGC TACGACACCT TGGCACCCCA CTTCCGCGTT
GACTGGTGCA ATGTGACCCT GGTGGATAAG TCAGTGCCGG AGCCTGCAGA TGAGGTGCCC
ACCCCAGGTG ATGGGATCCT GGAGCATGAC CCGTTCTTCT GCCCACCCAC TGAGGCCCCA
GACCGTGACT TCTTGGTGGA TGCTCTGGTC ACCCTCCTGG TGCCCCTGCT GGTGGCCCTG
CTTCTCACCT TGCTGCTGGC CTATGTCATG TGCTGCCGGC GGGAGGGAAG GCTGAAGAGA
GACCTGGCTA CCTCCGACAT CCAGATGGTC CACCACTGCA CCATCCACGG GAACACAGAG
GAGCTGCGGC AGATGGCGGC CAGCCGCGAG GTGCCCCGGC CACTCTCCAC CCTGCCCATG
TTCAATGTGC ACACAGTGGT TCCAGGTCCA GCCCTGACTT CATCCTCCCT TCTCTGTCCA
CACCACGAGT GGCACATCCC ACCTGCTGAT TCCAGCTCCT GGCCCTCCTG GAACCCAGGC
TCTAAACAAG CAGGGAGAGG GGGTGGGGTG GGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19381
Clone ID Related Accession (Same CDS sequence) NM_000023
Accession Version NM_000023.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1164bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product alpha-sarcoglycan isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from L34355.1, BC025702.1 and F25770.1. This sequence is a reference standard in the RefSeqGene project. On Oct 8, 2008 this sequence version replaced gi:4506910. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L34355.1, U08895.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGCTGAGA CACTCTTCTG GACTCCTCTC CTCGTGGTTC TCCTGGCAGG GCTGGGGGAC 
ACCGAGGCCC AGCAGACCAC GCTACACCCA CTTGTGGGCC GTGTCTTTGT GCACACCTTG
GACCATGAGA CGTTTCTGAG CCTTCCTGAG CATGTCGCTG TCCCACCCGC TGTCCACATC
ACCTACCACG CCCACCTCCA GGGACACCCA GACCTGCCCC GGTGGCTCCG CTACACCCAG
CGCAGCCCCC ACCACCCTGG CTTCCTCTAC GGCTCTGCCA CCCCAGAAGA TCGTGGGCTC
CAGGTCATTG AGGTCACAGC CTACAATCGG GACAGCTTTG ATACCACTCG GCAGAGGCTG
GTGCTGGAGA TTGGGGACCC AGAAGGCCCC CTGCTGCCAT ACCAAGCCGA GTTCCTGGTG
CGCAGCCACG ATGCGGAGGA GGTGCTGCCC TCAACACCTG CCAGCCGCTT CCTCTCAGCC
TTGGGGGGAC TCTGGGAGCC CGGAGAGCTT CAGCTGCTCA ACGTCACCTC TGCCTTGGAC
CGTGGGGGCC GTGTCCCCCT TCCCATTGAG GGCCGAAAAG AAGGGGTATA CATTAAGGTG
GGTTCTGCCT CACCTTTTTC TACTTGCCTG AAGATGGTGG CATCCCCCGA TAGCCACGCC
CGCTGTGCCC AGGGCCAGCC TCCACTTCTG TCTTGCTACG ACACCTTGGC ACCCCACTTC
CGCGTTGACT GGTGCAATGT GACCCTGGTG GATAAGTCAG TGCCGGAGCC TGCAGATGAG
GTGCCCACCC CAGGTGATGG GATCCTGGAG CATGACCCGT TCTTCTGCCC ACCCACTGAG
GCCCCAGACC GTGACTTCTT GGTGGATGCT CTGGTCACCC TCCTGGTGCC CCTGCTGGTG
GCCCTGCTTC TCACCTTGCT GCTGGCCTAT GTCATGTGCT GCCGGCGGGA GGGAAGGCTG
AAGAGAGACC TGGCTACCTC CGACATCCAG ATGGTCCACC ACTGCACCAT CCACGGGAAC
ACAGAGGAGC TGCGGCAGAT GGCGGCCAGC CGCGAGGTGC CCCGGCCACT CTCCACCCTG
CCCATGTTCA ATGTGCACAC AGGTGAGCGG CTGCCTCCCC GCGTGGACAG CGCCCAGGTG
CCCCTCATTC TGGACCAGCA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000014.1
CDS37..1200
Misc Feature(1)97..429(+)
Misc Feature(2)115..1197(+)
Misc Feature(3)130..423(+)
Misc Feature(4)907..969(+)
Exon (1)1..73
Gene:SGCA
Gene Synonym:
Exon (2)74..193
Gene:SGCA
Gene Synonym:
Exon (3)194..348
Gene:SGCA
Gene Synonym:
Exon (4)349..421
Gene:SGCA
Gene Synonym:
Exon (5)422..620
Gene:SGCA
Gene Synonym:
Exon (6)621..783
Gene:SGCA
Gene Synonym:
Exon (7)784..992
Gene:SGCA
Gene Synonym:
Exon (8)993..1019
Gene:SGCA
Gene Synonym:
Exon (9)1020..1212
Gene:SGCA
Gene Synonym:
Exon (10)1213..1433
Gene:SGCA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000023
Organism Homo sapiens (human)
Definition Homo sapiens sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein) (SGCA), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000023

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGCTGAGA CACTCTTCTG GACTCCTCTC CTCGTGGTTC TCCTGGCAGG GCTGGGGGAC 
ACCGAGGCCC AGCAGACCAC GCTACACCCA CTTGTGGGCC GTGTCTTTGT GCACACCTTG
GACCATGAGA CGTTTCTGAG CCTTCCTGAG CATGTCGCTG TCCCACCCGC TGTCCACATC
ACCTACCACG CCCACCTCCA GGGACACCCA GACCTGCCCC GGTGGCTCCG CTACACCCAG
CGCAGCCCCC ACCACCCTGG CTTCCTCTAC GGCTCTGCCA CCCCAGAAGA TCGTGGGCTC
CAGGTCATTG AGGTCACAGC CTACAATCGG GACAGCTTTG ATACCACTCG GCAGAGGCTG
GTGCTGGAGA TTGGGGACCC AGAAGGCCCC CTGCTGCCAT ACCAAGCCGA GTTCCTGGTG
CGCAGCCACG ATGCGGAGGA GGTGCTGCCC TCAACACCTG CCAGCCGCTT CCTCTCAGCC
TTGGGGGGAC TCTGGGAGCC CGGAGAGCTT CAGCTGCTCA ACGTCACCTC TGCCTTGGAC
CGTGGGGGCC GTGTCCCCCT TCCCATTGAG GGCCGAAAAG AAGGGGTATA CATTAAGGTG
GGTTCTGCCT CACCTTTTTC TACTTGCCTG AAGATGGTGG CATCCCCCGA TAGCCACGCC
CGCTGTGCCC AGGGCCAGCC TCCACTTCTG TCTTGCTACG ACACCTTGGC ACCCCACTTC
CGCGTTGACT GGTGCAATGT GACCCTGGTG GATAAGTCAG TGCCGGAGCC TGCAGATGAG
GTGCCCACCC CAGGTGATGG GATCCTGGAG CATGACCCGT TCTTCTGCCC ACCCACTGAG
GCCCCAGACC GTGACTTCTT GGTGGATGCT CTGGTCACCC TCCTGGTGCC CCTGCTGGTG
GCCCTGCTTC TCACCTTGCT GCTGGCCTAT GTCATGTGCT GCCGGCGGGA GGGAAGGCTG
AAGAGAGACC TGGCTACCTC CGACATCCAG ATGGTCCACC ACTGCACCAT CCACGGGAAC
ACAGAGGAGC TGCGGCAGAT GGCGGCCAGC CGCGAGGTGC CCCGGCCACT CTCCACCCTG
CCCATGTTCA ATGTGCACAC AGGTGAGCGG CTGCCTCCCC GCGTGGACAG CGCCCAGGTG
CCCCTCATTC TGGACCAGCA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu31407
Clone ID Related Accession (Same CDS sequence) NM_001135697
Accession Version NM_001135697.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 792bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product alpha-sarcoglycan isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from L35853.1 and F25770.1. Transcript Variant: This variant (2) lacks two alternate in-frame exons in the central coding region, compared to variant 1. The resulting isoform (2) lacks a segment of the sarcoglycan domain including the transmembrane domain, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L35853.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCTGAGA CACTCTTCTG GACTCCTCTC CTCGTGGTTC TCCTGGCAGG GCTGGGGGAC 
ACCGAGGCCC AGCAGACCAC GCTACACCCA CTTGTGGGCC GTGTCTTTGT GCACACCTTG
GACCATGAGA CGTTTCTGAG CCTTCCTGAG CATGTCGCTG TCCCACCCGC TGTCCACATC
ACCTACCACG CCCACCTCCA GGGACACCCA GACCTGCCCC GGTGGCTCCG CTACACCCAG
CGCAGCCCCC ACCACCCTGG CTTCCTCTAC GGCTCTGCCA CCCCAGAAGA TCGTGGGCTC
CAGGTCATTG AGGTCACAGC CTACAATCGG GACAGCTTTG ATACCACTCG GCAGAGGCTG
GTGCTGGAGA TTGGGGACCC AGAAGGCCCC CTGCTGCCAT ACCAAGCCGA GTTCCTGGTG
CGCAGCCACG ATGCGGAGGA GGTGCTGCCC TCAACACCTG CCAGCCGCTT CCTCTCAGCC
TTGGGGGGAC TCTGGGAGCC CGGAGAGCTT CAGCTGCTCA ACGTCACCTC TGCCTTGGAC
CGTGGGGGCC GTGTCCCCCT TCCCATTGAG GGCCGAAAAG AAGGGCTGAA GAGAGACCTG
GCTACCTCCG ACATCCAGAT GGTCCACCAC TGCACCATCC ACGGGAACAC AGAGGAGCTG
CGGCAGATGG CGGCCAGCCG CGAGGTGCCC CGGCCACTCT CCACCCTGCC CATGTTCAAT
GTGCACACAG GTGAGCGGCT GCCTCCCCGC GTGGACAGCG CCCAGGTGCC CCTCATTCTG
GACCAGCACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001129169.1
CDS37..828
Misc Feature(1)97..429(+)
Misc Feature(2)115..825(+)
Misc Feature(3)130..423(+)
Exon (1)1..73
Gene:SGCA
Gene Synonym:
Exon (2)74..193
Gene:SGCA
Gene Synonym:
Exon (3)194..348
Gene:SGCA
Gene Synonym:
Exon (4)349..421
Gene:SGCA
Gene Synonym:
Exon (5)422..620
Gene:SGCA
Gene Synonym:
Exon (6)621..647
Gene:SGCA
Gene Synonym:
Exon (7)648..840
Gene:SGCA
Gene Synonym:
Exon (8)841..1061
Gene:SGCA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001135697
Organism Homo sapiens (human)
Definition Homo sapiens sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein) (SGCA), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001135697

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCTGAGA CACTCTTCTG GACTCCTCTC CTCGTGGTTC TCCTGGCAGG GCTGGGGGAC 
ACCGAGGCCC AGCAGACCAC GCTACACCCA CTTGTGGGCC GTGTCTTTGT GCACACCTTG
GACCATGAGA CGTTTCTGAG CCTTCCTGAG CATGTCGCTG TCCCACCCGC TGTCCACATC
ACCTACCACG CCCACCTCCA GGGACACCCA GACCTGCCCC GGTGGCTCCG CTACACCCAG
CGCAGCCCCC ACCACCCTGG CTTCCTCTAC GGCTCTGCCA CCCCAGAAGA TCGTGGGCTC
CAGGTCATTG AGGTCACAGC CTACAATCGG GACAGCTTTG ATACCACTCG GCAGAGGCTG
GTGCTGGAGA TTGGGGACCC AGAAGGCCCC CTGCTGCCAT ACCAAGCCGA GTTCCTGGTG
CGCAGCCACG ATGCGGAGGA GGTGCTGCCC TCAACACCTG CCAGCCGCTT CCTCTCAGCC
TTGGGGGGAC TCTGGGAGCC CGGAGAGCTT CAGCTGCTCA ACGTCACCTC TGCCTTGGAC
CGTGGGGGCC GTGTCCCCCT TCCCATTGAG GGCCGAAAAG AAGGGCTGAA GAGAGACCTG
GCTACCTCCG ACATCCAGAT GGTCCACCAC TGCACCATCC ACGGGAACAC AGAGGAGCTG
CGGCAGATGG CGGCCAGCCG CGAGGTGCCC CGGCCACTCT CCACCCTGCC CATGTTCAAT
GTGCACACAG GTGAGCGGCT GCCTCCCCGC GTGGACAGCG CCCAGGTGCC CCTCATTCTG
GACCAGCACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
*
*
*
*