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SGSH cDNA ORF clone, Homo sapiens(Human)

Gene Symbol SGSH
Entrez Gene ID 6448
Full Name N-sulfoglucosamine sulfohydrolase
Synonyms HSS,MPS3A,SFMD
General protein information
Preferred Names

N-sulfoglucosamine sulfohydrolase

Names

N-sulphoglucosamine sulphohydrolase
heparan sulfate sulfatase
mucopolysaccharidosis type IIIA
sulfoglucosamine sulfamidase
sulphamidase

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

17

17q25.3

Summary This gene encodes one of several enzymes involved in the lysosomal degradation of heparan sulfate. Mutations in this gene are associated with Sanfilippo syndrome A, one type of the lysosomal storage disease mucopolysaccaridosis III, which results from impaired degradation of heparan sulfate. Transcripts of varying sizes have been reported but their biological validity has not been determined. [provided by RefSeq, Jul 2008].
Disorder MIM:

605270

Disorder Html: Mucopolysaccharidisis type IIIA (Sanfilippo A), 252900 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_000199.3 NP_000190.1 N-sulphoglucosamine sulphohydrolase precursor
XM_005257582.3 XP_005257639.1 N-sulphoglucosamine sulphohydrolase isoform X1
XM_017024951.1 XP_016880440.1 N-sulphoglucosamine sulphohydrolase isoform X3
XM_011525126.1 XP_011523428.1 N-sulphoglucosamine sulphohydrolase isoform X4
XM_005257583.4 XP_005257640.1 N-sulphoglucosamine sulphohydrolase isoform X2
XM_017024952.1 XP_016880441.1 N-sulphoglucosamine sulphohydrolase isoform X5
XM_011525127.2 XP_011523429.1 N-sulphoglucosamine sulphohydrolase isoform X6



Macaca mulatta (rhesus monkey) SGSH XP_001110101.2
Canis lupus familiaris (dog) SGSH NP_001003114.1
Bos taurus (cattle) SGSH NP_001095659.2
Xenopus tropicalis (tropical clawed frog) sgsh NP_001120065.1
Homo sapiens (human) SGSH NP_000190.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP004205 XP_313106.2
Mus musculus (house mouse) Sgsh NP_061292.2
Gallus gallus (chicken) SGSH XP_420084.4
Danio rerio (zebrafish) sgsh NP_001116740.1
Drosophila melanogaster (fruit fly) CG14291 NP_650760.1


Related articles in PubMed

Broad functional correction of molecular impairments by systemic delivery of scAAVrh74-hSGSH gene delivery in MPS IIIA mice.
Duncan FJ, Naughton BJ, Zaraspe K, Murrey DA, Meadows AS, Clark KR, Newsom DE, White P, Fu H, McCarty DM
Molecular therapy : the journal of the American Society of Gene Therapy23(4)638-47(2015 Apr)

Structure of sulfamidase provides insight into the molecular pathology of mucopolysaccharidosis IIIA.
Sidhu NS, Schreiber K, Pröpper K, Becker S, Usón I, Sheldrick GM, Gärtner J, Krätzner R, Steinfeld R
Acta crystallographica. Section D, Biological crystallography70(Pt 5)1321-35(2014 May)

Enzyme replacement reduces neuropathology in MPS IIIA dogs.
Crawley AC, Marshall N, Beard H, Hassiotis S, Walsh V, King B, Hucker N, Fuller M, Jolly RD, Hopwood JJ, Hemsley KM
Neurobiology of disease43(2)422-34(2011 Aug)

Residual activity and proteasomal degradation of p.Ser298Pro sulfamidase identified in patients with a mild clinical phenotype of Sanfilippo A syndrome.
Muschol N, Pohl S, Meyer A, Gal A, Ullrich K, Braulke T
American journal of medical genetics. Part A155A(7)1634-9(2011 Jul)

New genetic associations detected in a host response study to hepatitis B vaccine.
Davila S, Froeling FE, Tan A, Bonnard C, Boland GJ, Snippe H, Hibberd ML, Seielstad M
Genes and immunity11(3)232-8(2010 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results demonstrate that a single systemic scAAVrh74-hSGSH delivery mediated efficient restoration of SGSH activity and resulted in a near complete correction of MPS IIIA molecular pathology
Title: Broad functional correction of molecular impairments by systemic delivery of scAAVrh74-hSGSH gene delivery in MPS IIIA mice.

The crystal structure of glycosylated sulfamidase provides insight into the diverse effects of pathogenic mutations on sulfamidase function in mucopolysaccharidosis type IIIA.
Title: Structure of sulfamidase provides insight into the molecular pathology of mucopolysaccharidosis IIIA.

Pre-symptomatic treatment of progressive neurodegenerative disease (mucopolysaccharidosis type IIIA) via intra-cerebrospinal fluid injection of recombinant human SGSH mediates highly significant reductions in neuropathology in a canine model.
Title: Enzyme replacement reduces neuropathology in MPS IIIA dogs.

Processing and secretion of p.Ser298Pro sulfamidase suggests that small amounts of the newly synthesized enzyme are transported to lysosomes
Title: Residual activity and proteasomal degradation of p.Ser298Pro sulfamidase identified in patients with a mild clinical phenotype of Sanfilippo A syndrome.

Observational study of gene-disease association. (HuGE Navigator)
Title: New genetic associations detected in a host response study to hepatitis B vaccine.

The following SGSH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SGSH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu18039 NM_000199.3
Latest version!
Homo sapiens N-sulfoglucosamine sulfohydrolase (SGSH), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu50585 XM_005257582.3
Latest version!
Homo sapiens N-sulfoglucosamine sulfohydrolase (SGSH), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu50586 XM_005257583.4
Latest version!
Homo sapiens N-sulfoglucosamine sulfohydrolase (SGSH), transcript variant X11, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu60463 XM_011525126.1
Latest version!
Homo sapiens N-sulfoglucosamine sulfohydrolase (SGSH), transcript variant X13, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu60464 XM_011525127.2
Latest version!
Homo sapiens N-sulfoglucosamine sulfohydrolase (SGSH), transcript variant X15, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu87041 XM_017024951.1
Latest version!
Homo sapiens N-sulfoglucosamine sulfohydrolase (SGSH), transcript variant X12, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $223.30
$319.00
OHu87042 XM_017024952.1
Latest version!
Homo sapiens N-sulfoglucosamine sulfohydrolase (SGSH), transcript variant X14, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $223.30
$319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18039
Clone ID Related Accession (Same CDS sequence) NM_000199.3
Accession Version NM_000199.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1509bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-03
Organism Homo sapiens(Human)
Product N-sulphoglucosamine sulphohydrolase precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC319407.1, U30894.1, AC087741.18, AW274014.1 and BF445930.1. This sequence is a reference standard in the RefSeqGene project. On Apr 12, 2008 this sequence version replaced NM_000199.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.241459.1, SRR1803611.119830.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAGCTGCC CCGTGCCCGC CTGCTGCGCG CTGCTGCTAG TCCTGGGGCT CTGCCGGGCG 
CGTCCCCGGA ACGCACTGCT GCTCCTCGCG GATGACGGAG GCTTTGAGAG TGGCGCGTAC
AACAACAGCG CCATCGCCAC CCCGCACCTG GACGCCTTGG CCCGCCGCAG CCTCCTCTTT
CGCAATGCCT TCACCTCGGT CAGCAGCTGC TCTCCCAGCC GCGCCAGCCT CCTCACTGGC
CTGCCCCAGC ATCAGAATGG GATGTACGGG CTGCACCAGG ACGTGCACCA CTTCAACTCC
TTCGACAAGG TGCGGAGCCT GCCGCTGCTG CTCAGCCAAG CTGGTGTGCG CACAGGCATC
ATCGGGAAGA AGCACGTGGG GCCGGAGACC GTGTACCCGT TTGACTTTGC GTACACGGAG
GAGAATGGCT CCGTCCTCCA GGTGGGGCGG AACATCACTA GAATTAAGCT GCTCGTCCGG
AAATTCCTGC AGACTCAGGA TGACCGGCCT TTCTTCCTCT ACGTCGCCTT CCACGACCCC
CACCGCTGTG GGCACTCCCA GCCCCAGTAC GGAACCTTCT GTGAGAAGTT TGGCAACGGA
GAGAGCGGCA TGGGTCGTAT CCCAGACTGG ACCCCCCAGG CCTACGACCC ACTGGACGTG
CTGGTGCCTT ACTTCGTCCC CAACACCCCG GCAGCCCGAG CCGACCTGGC CGCTCAGTAC
ACCACCGTCG GCCGCATGGA CCAAGGAGTT GGACTGGTGC TCCAGGAGCT GCGTGACGCC
GGTGTCCTGA ACGACACACT GGTGATCTTC ACGTCCGACA ACGGGATCCC CTTCCCCAGC
GGCAGGACCA ACCTGTACTG GCCGGGCACT GCTGAACCCT TACTGGTGTC ATCCCCGGAG
CACCCAAAAC GCTGGGGCCA AGTCAGCGAG GCCTACGTGA GCCTCCTAGA CCTCACGCCC
ACCATCTTGG ATTGGTTCTC GATCCCGTAC CCCAGCTACG CCATCTTTGG CTCGAAGACC
ATCCACCTCA CTGGCCGGTC CCTCCTGCCG GCGCTGGAGG CCGAGCCCCT CTGGGCCACC
GTCTTTGGCA GCCAGAGCCA CCACGAGGTC ACCATGTCCT ACCCCATGCG CTCCGTGCAG
CACCGGCACT TCCGCCTCGT GCACAACCTC AACTTCAAGA TGCCCTTTCC CATCGACCAG
GACTTCTACG TCTCACCCAC CTTCCAGGAC CTCCTGAACC GCACCACAGC TGGTCAGCCC
ACGGGCTGGT ACAAGGACCT CCGTCATTAC TACTACCGGG CGCGCTGGGA GCTCTACGAC
CGGAGCCGGG ACCCCCACGA GACCCAGAAC CTGGCCACCG ACCCGCGCTT TGCTCAGCTT
CTGGAGATGC TTCGGGACCA GCTGGCCAAG TGGCAGTGGG AGACCCACGA CCCCTGGGTG
TGCGCCCCCG ACGGCGTCCT GGAGGAGAAG CTCTCTCCCC AGTGCCAGCC CCTCCACAAT
GAGCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000190.1
CDS88..1596
Misc Feature(1)145..1419
Misc Feature(2)157..1503
Misc Feature(3)order(178..183,295..297,454..456,460..462,628..630,904..909)
Misc Feature(4)order(178..183,295..297,454..456,460..462,628..630,907..909)
Misc Feature(5)order(178..183,295..297,904..909)
Misc Feature(6)295..297
Misc Feature(7)877..879
Misc Feature(8)order(1186..1188,1255..1260)
Exon (1)1..175
Exon (2)176..336
Exon (3)337..442
Exon (4)443..593
Exon (5)594..750
Exon (6)751..832
Exon (7)833..1036
Exon (8)1037..2768
Translation

Target ORF information:

RefSeq Version NM_000199.3
Organism Homo sapiens(Human)
Definition Homo sapiens N-sulfoglucosamine sulfohydrolase (SGSH), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000199.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAGCTGCC CCGTGCCCGC CTGCTGCGCG CTGCTGCTAG TCCTGGGGCT CTGCCGGGCG 
CGTCCCCGGA ACGCACTGCT GCTCCTCGCG GATGACGGAG GCTTTGAGAG TGGCGCGTAC
AACAACAGCG CCATCGCCAC CCCGCACCTG GACGCCTTGG CCCGCCGCAG CCTCCTCTTT
CGCAATGCCT TCACCTCGGT CAGCAGCTGC TCTCCCAGCC GCGCCAGCCT CCTCACTGGC
CTGCCCCAGC ATCAGAATGG GATGTACGGG CTGCACCAGG ACGTGCACCA CTTCAACTCC
TTCGACAAGG TGCGGAGCCT GCCGCTGCTG CTCAGCCAAG CTGGTGTGCG CACAGGCATC
ATCGGGAAGA AGCACGTGGG GCCGGAGACC GTGTACCCGT TTGACTTTGC GTACACGGAG
GAGAATGGCT CCGTCCTCCA GGTGGGGCGG AACATCACTA GAATTAAGCT GCTCGTCCGG
AAATTCCTGC AGACTCAGGA TGACCGGCCT TTCTTCCTCT ACGTCGCCTT CCACGACCCC
CACCGCTGTG GGCACTCCCA GCCCCAGTAC GGAACCTTCT GTGAGAAGTT TGGCAACGGA
GAGAGCGGCA TGGGTCGTAT CCCAGACTGG ACCCCCCAGG CCTACGACCC ACTGGACGTG
CTGGTGCCTT ACTTCGTCCC CAACACCCCG GCAGCCCGAG CCGACCTGGC CGCTCAGTAC
ACCACCGTCG GCCGCATGGA CCAAGGAGTT GGACTGGTGC TCCAGGAGCT GCGTGACGCC
GGTGTCCTGA ACGACACACT GGTGATCTTC ACGTCCGACA ACGGGATCCC CTTCCCCAGC
GGCAGGACCA ACCTGTACTG GCCGGGCACT GCTGAACCCT TACTGGTGTC ATCCCCGGAG
CACCCAAAAC GCTGGGGCCA AGTCAGCGAG GCCTACGTGA GCCTCCTAGA CCTCACGCCC
ACCATCTTGG ATTGGTTCTC GATCCCGTAC CCCAGCTACG CCATCTTTGG CTCGAAGACC
ATCCACCTCA CTGGCCGGTC CCTCCTGCCG GCGCTGGAGG CCGAGCCCCT CTGGGCCACC
GTCTTTGGCA GCCAGAGCCA CCACGAGGTC ACCATGTCCT ACCCCATGCG CTCCGTGCAG
CACCGGCACT TCCGCCTCGT GCACAACCTC AACTTCAAGA TGCCCTTTCC CATCGACCAG
GACTTCTACG TCTCACCCAC CTTCCAGGAC CTCCTGAACC GCACCACAGC TGGTCAGCCC
ACGGGCTGGT ACAAGGACCT CCGTCATTAC TACTACCGGG CGCGCTGGGA GCTCTACGAC
CGGAGCCGGG ACCCCCACGA GACCCAGAAC CTGGCCACCG ACCCGCGCTT TGCTCAGCTT
CTGGAGATGC TTCGGGACCA GCTGGCCAAG TGGCAGTGGG AGACCCACGA CCCCTGGGTG
TGCGCCCCCG ACGGCGTCCT GGAGGAGAAG CTCTCTCCCC AGTGCCAGCC CCTCCACAAT
GAGCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50585
Clone ID Related Accession (Same CDS sequence) XM_005257582.3
Accession Version XM_005257582.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1089bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product N-sulphoglucosamine sulphohydrolase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005257582.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGAGCTGCC CCGTGCCCGC CTGCTGCGCG CTGCTGCTAG TCCTGGGGCT CTGCCGGGCG 
CGTCCCCGGA ACGCACTGCT GCTCCTCGCG GATGACGGAG GCTTTGAGAG TGGCGCGTAC
AACAACAGCG CCATCGCCAC CCCGCACCTG GACGCCTTGG CCCGCCGCAG CCTCCTCTTT
CGCAATGCCT TCACCTCGGT CAGCAGCTGC TCTCCCAGCC GCGCCAGCCT CCTCACTGGC
CTGCCCCAGC ATCAGAATGG GATGTACGGG CTGCACCAGG ACGTGCACCA CTTCAACTCC
TTCGACAAGG TGCGGAGCCT GCCGCTGCTG CTCAGCCAAG CTGGTGTGCG CACAGGCATC
ATCGGGAAGA AGCACGTGGG GCCGGAGACC GTGTACCCGT TTGACTTTGC GTACACGGAG
GAGAATGGCT CCGTCCTCCA GGTGGGGCGG AACATCACTA GAATTAAGCT GCTCGTCCGG
AAATTCCTGC AGACTCAGGA TGACCGGCCT TTCTTCCTCT ACGTCGCCTT CCACGACCCC
CACCGCTGTG GGCACTCCCA GCCCCAGTAC GGAACCTTCT GTGAGAAGTT TGGCAACGGA
GAGAGCGGCA TGGGTCGTAT CCCAGACTGG ACCCCCCAGG CCTACGACCC ACTGGACGTG
CTGGTGCCTT ACTTCGTCCC CAACACCCCG GCAGCCCGAG CCGACCTGGC CGCTCAGTAC
ACCACCGTCG GCCGCATGGA CCAAGGAGTT GGACTGGTGC TCCAGGAGCT GCGTGACGCC
GGTGTCCTGA ACGACACACT GGTGATCTTC ACGTCCGACA ACGGGATCCC CTTCCCCAGC
GGCAGGACCA ACCTGTACTG GCCGGGCACT GCTGAACCCT TACTGGTGTC ATCCCCGGAG
CACCCAAAAC GCTGGGGCCA AGTCAGCGAG GCCTACGTGA GCCTCCTAGG CACTGAGGCC
CAGAGAGATG ACGTGGCGGT GAGAGGTGTC GGCTCGACAA GGCACCTCCC GCTGCTGATT
GGATTGGAGA AGGGAGCCGC CCAGAGTGGC GTAGGTTCGG CCCTTCCGAG GGCAGCTCCT
GTGTGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005257639.1
CDS21..1109
Misc Feature(1)78..>968
Misc Feature(2)90..>968
Misc Feature(3)order(111..116,228..230,387..389,393..395,561..563,837..842)
Misc Feature(4)order(111..116,228..230,387..389,393..395,561..563,840..842)
Misc Feature(5)order(111..116,228..230,837..842)
Translation

Target ORF information:

RefSeq Version XM_005257582.3
Organism Homo sapiens(Human)
Definition Homo sapiens N-sulfoglucosamine sulfohydrolase (SGSH), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005257582.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGAGCTGCC CCGTGCCCGC CTGCTGCGCG CTGCTGCTAG TCCTGGGGCT CTGCCGGGCG 
CGTCCCCGGA ACGCACTGCT GCTCCTCGCG GATGACGGAG GCTTTGAGAG TGGCGCGTAC
AACAACAGCG CCATCGCCAC CCCGCACCTG GACGCCTTGG CCCGCCGCAG CCTCCTCTTT
CGCAATGCCT TCACCTCGGT CAGCAGCTGC TCTCCCAGCC GCGCCAGCCT CCTCACTGGC
CTGCCCCAGC ATCAGAATGG GATGTACGGG CTGCACCAGG ACGTGCACCA CTTCAACTCC
TTCGACAAGG TGCGGAGCCT GCCGCTGCTG CTCAGCCAAG CTGGTGTGCG CACAGGCATC
ATCGGGAAGA AGCACGTGGG GCCGGAGACC GTGTACCCGT TTGACTTTGC GTACACGGAG
GAGAATGGCT CCGTCCTCCA GGTGGGGCGG AACATCACTA GAATTAAGCT GCTCGTCCGG
AAATTCCTGC AGACTCAGGA TGACCGGCCT TTCTTCCTCT ACGTCGCCTT CCACGACCCC
CACCGCTGTG GGCACTCCCA GCCCCAGTAC GGAACCTTCT GTGAGAAGTT TGGCAACGGA
GAGAGCGGCA TGGGTCGTAT CCCAGACTGG ACCCCCCAGG CCTACGACCC ACTGGACGTG
CTGGTGCCTT ACTTCGTCCC CAACACCCCG GCAGCCCGAG CCGACCTGGC CGCTCAGTAC
ACCACCGTCG GCCGCATGGA CCAAGGAGTT GGACTGGTGC TCCAGGAGCT GCGTGACGCC
GGTGTCCTGA ACGACACACT GGTGATCTTC ACGTCCGACA ACGGGATCCC CTTCCCCAGC
GGCAGGACCA ACCTGTACTG GCCGGGCACT GCTGAACCCT TACTGGTGTC ATCCCCGGAG
CACCCAAAAC GCTGGGGCCA AGTCAGCGAG GCCTACGTGA GCCTCCTAGG CACTGAGGCC
CAGAGAGATG ACGTGGCGGT GAGAGGTGTC GGCTCGACAA GGCACCTCCC GCTGCTGATT
GGATTGGAGA AGGGAGCCGC CCAGAGTGGC GTAGGTTCGG CCCTTCCGAG GGCAGCTCCT
GTGTGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50586
Clone ID Related Accession (Same CDS sequence) XM_005257583.4
Accession Version XM_005257583.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1023bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product N-sulphoglucosamine sulphohydrolase isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005257583.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGAGCTGCC CCGTGCCCGC CTGCTGCGCG CTGCTGCTAG TCCTGGGGCT CTGCCGGGCG 
CGTCCCCGGA ACGCACTGCT GCTCCTCGCG GATGACGGAG GCTTTGAGAG TGGCGCGTAC
AACAACAGCG CCATCGCCAC CCCGCACCTG GACGCCTTGG CCCGCCGCAG CCTCCTCTTT
CGCAATGCCT TCACCTCGGT CAGCAGCTGC TCTCCCAGCC GCGCCAGCCT CCTCACTGGC
CTGCCCCAGC ATCAGAATGG GATGTACGGG CTGCACCAGG ACGTGCACCA CTTCAACTCC
TTCGACAAGG TGCGGAGCCT GCCGCTGCTG CTCAGCCAAG CTGGTGTGCG CACAGGCATC
ATCGGGAAGA AGCACGTGGG GCCGGAGACC GTGTACCCGT TTGACTTTGC GTACACGGAG
GAGAATGGCT CCGTCCTCCA GGTGGGGCGG AACATCACTA GAATTAAGCT GCTCGTCCGG
AAATTCCTGC AGACTCAGGA TGACCGGCCT TTCTTCCTCT ACGTCGCCTT CCACGACCCC
CACCGCTGTG GGCACTCCCA GCCCCAGTAC GGAACCTTCT GTGAGAAGTT TGGCAACGGA
GAGAGCGGCA TGGGTCGTAT CCCAGACTGG ACCCCCCAGG CCTACGACCC ACTGGACGTG
CTGGTGCCTT ACTTCGTCCC CAACACCCCG GCAGCCCGAG CCGACCTGGC CGCTCAGTAC
ACCACCGTCG GCCGCATGGA CCAAGGAGTT GGACTGGTGC TCCAGGAGCT GCGTGACGCC
GGTGTCCTGA ACGACACACT GGTGATCTTC ACGTCCGACA ACGGGATCCC CTTCCCCAGC
GGCAGGACCA ACCTGTACTG GCCGGGCACT GCTGAACCCT TACTGGTGTC ATCCCCGGAG
CACCCAAAAC GCTGGGGCCA AGTCAGCGAG GCCTACGTGA GCCTCCTAGT GACTAGGCTA
CTTTTGCCCA CTGTAAAAGG CACCGTCATC CTGGTATCAA GATTCTGGAG AGGCCAGATT
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005257640.1
CDS21..1043
Misc Feature(1)78..>995
Misc Feature(2)90..>995
Misc Feature(3)order(111..116,228..230,387..389,393..395,561..563,837..842)
Misc Feature(4)order(111..116,228..230,387..389,393..395,561..563,840..842)
Misc Feature(5)order(111..116,228..230,837..842)
Translation

Target ORF information:

RefSeq Version XM_005257583.4
Organism Homo sapiens(Human)
Definition Homo sapiens N-sulfoglucosamine sulfohydrolase (SGSH), transcript variant X11, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005257583.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGAGCTGCC CCGTGCCCGC CTGCTGCGCG CTGCTGCTAG TCCTGGGGCT CTGCCGGGCG 
CGTCCCCGGA ACGCACTGCT GCTCCTCGCG GATGACGGAG GCTTTGAGAG TGGCGCGTAC
AACAACAGCG CCATCGCCAC CCCGCACCTG GACGCCTTGG CCCGCCGCAG CCTCCTCTTT
CGCAATGCCT TCACCTCGGT CAGCAGCTGC TCTCCCAGCC GCGCCAGCCT CCTCACTGGC
CTGCCCCAGC ATCAGAATGG GATGTACGGG CTGCACCAGG ACGTGCACCA CTTCAACTCC
TTCGACAAGG TGCGGAGCCT GCCGCTGCTG CTCAGCCAAG CTGGTGTGCG CACAGGCATC
ATCGGGAAGA AGCACGTGGG GCCGGAGACC GTGTACCCGT TTGACTTTGC GTACACGGAG
GAGAATGGCT CCGTCCTCCA GGTGGGGCGG AACATCACTA GAATTAAGCT GCTCGTCCGG
AAATTCCTGC AGACTCAGGA TGACCGGCCT TTCTTCCTCT ACGTCGCCTT CCACGACCCC
CACCGCTGTG GGCACTCCCA GCCCCAGTAC GGAACCTTCT GTGAGAAGTT TGGCAACGGA
GAGAGCGGCA TGGGTCGTAT CCCAGACTGG ACCCCCCAGG CCTACGACCC ACTGGACGTG
CTGGTGCCTT ACTTCGTCCC CAACACCCCG GCAGCCCGAG CCGACCTGGC CGCTCAGTAC
ACCACCGTCG GCCGCATGGA CCAAGGAGTT GGACTGGTGC TCCAGGAGCT GCGTGACGCC
GGTGTCCTGA ACGACACACT GGTGATCTTC ACGTCCGACA ACGGGATCCC CTTCCCCAGC
GGCAGGACCA ACCTGTACTG GCCGGGCACT GCTGAACCCT TACTGGTGTC ATCCCCGGAG
CACCCAAAAC GCTGGGGCCA AGTCAGCGAG GCCTACGTGA GCCTCCTAGT GACTAGGCTA
CTTTTGCCCA CTGTAAAAGG CACCGTCATC CTGGTATCAA GATTCTGGAG AGGCCAGATT
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60463
Clone ID Related Accession (Same CDS sequence) XM_011525126.1
Accession Version XM_011525126.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1011bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product N-sulphoglucosamine sulphohydrolase isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGAGCTGCC CCGTGCCCGC CTGCTGCGCG CTGCTGCTAG TCCTGGGGCT CTGCCGGGCG 
CGTCCCCGGA ACGCACTGCT GCTCCTCGCG GATGACGGAG GCTTTGAGAG TGGCGCGTAC
AACAACAGCG CCATCGCCAC CCCGCACCTG GACGCCTTGG CCCGCCGCAG CCTCCTCTTT
CGCAATGCCT TCACCTCGGT CAGCAGCTGC TCTCCCAGCC GCGCCAGCCT CCTCACTGGC
CTGCCCCAGC ATCAGAATGG GATGTACGGG CTGCACCAGG ACGTGCACCA CTTCAACTCC
TTCGACAAGG TGCGGAGCCT GCCGCTGCTG CTCAGCCAAG CTGGTGTGCG CACAGGCATC
ATCGGGAAGA AGCACGTGGG GCCGGAGACC GTGTACCCGT TTGACTTTGC GTACACGGAG
GAGAATGGCT CCGTCCTCCA GGTGGGGCGG AACATCACTA GAATTAAGCT GCTCGTCCGG
AAATTCCTGC AGACTCAGGA TGACCGGCCT TTCTTCCTCT ACGTCGCCTT CCACGACCCC
CACCGCTGTG GGCACTCCCA GCCCCAGTAC GGAACCTTCT GTGAGAAGTT TGGCAACGGA
GAGAGCGGCA TGGGTCGTAT CCCAGACTGG ACCCCCCAGG CCTACGACCC ACTGGACGTG
CTGGTGCCTT ACTTCGTCCC CAACACCCCG GCAGCCCGAG CCGACCTGGC CGCTCAGTAC
ACCACCGTCG GCCGCATGGA CCAAGGAGTT GGACTGGTGC TCCAGGAGCT GCGTGACGCC
GGTGTCCTGA ACGACACACT GGTGATCTTC ACGTCCGACA ACGGGATCCC CTTCCCCAGC
GGCAGGACCA ACCTGTACTG GCCGGGCACT GCTGAACCCT TACTGGTGTC ATCCCCGGAG
CACCCAAAAC GCTGGGGCCA AGTCAGCGAG GCCTACGTGA GCCTCCTAGA GGAAAAGAAT
GAGGCTCAGA GAGGTGAAGT AAGCTGCTCA GGATTGCACA GCTGGCACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011523428.1
CDS21..1031
Misc Feature(1)78..>968
Misc Feature(2)90..>968
Misc Feature(3)order(111..116,228..230,387..389,393..395,561..563,837..842)
Misc Feature(4)order(111..116,228..230,387..389,393..395,561..563,840..842)
Misc Feature(5)order(111..116,228..230,837..842)
Translation

Target ORF information:

RefSeq Version XM_011525126.1
Organism Homo sapiens(Human)
Definition Homo sapiens N-sulfoglucosamine sulfohydrolase (SGSH), transcript variant X13, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011525126.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGAGCTGCC CCGTGCCCGC CTGCTGCGCG CTGCTGCTAG TCCTGGGGCT CTGCCGGGCG 
CGTCCCCGGA ACGCACTGCT GCTCCTCGCG GATGACGGAG GCTTTGAGAG TGGCGCGTAC
AACAACAGCG CCATCGCCAC CCCGCACCTG GACGCCTTGG CCCGCCGCAG CCTCCTCTTT
CGCAATGCCT TCACCTCGGT CAGCAGCTGC TCTCCCAGCC GCGCCAGCCT CCTCACTGGC
CTGCCCCAGC ATCAGAATGG GATGTACGGG CTGCACCAGG ACGTGCACCA CTTCAACTCC
TTCGACAAGG TGCGGAGCCT GCCGCTGCTG CTCAGCCAAG CTGGTGTGCG CACAGGCATC
ATCGGGAAGA AGCACGTGGG GCCGGAGACC GTGTACCCGT TTGACTTTGC GTACACGGAG
GAGAATGGCT CCGTCCTCCA GGTGGGGCGG AACATCACTA GAATTAAGCT GCTCGTCCGG
AAATTCCTGC AGACTCAGGA TGACCGGCCT TTCTTCCTCT ACGTCGCCTT CCACGACCCC
CACCGCTGTG GGCACTCCCA GCCCCAGTAC GGAACCTTCT GTGAGAAGTT TGGCAACGGA
GAGAGCGGCA TGGGTCGTAT CCCAGACTGG ACCCCCCAGG CCTACGACCC ACTGGACGTG
CTGGTGCCTT ACTTCGTCCC CAACACCCCG GCAGCCCGAG CCGACCTGGC CGCTCAGTAC
ACCACCGTCG GCCGCATGGA CCAAGGAGTT GGACTGGTGC TCCAGGAGCT GCGTGACGCC
GGTGTCCTGA ACGACACACT GGTGATCTTC ACGTCCGACA ACGGGATCCC CTTCCCCAGC
GGCAGGACCA ACCTGTACTG GCCGGGCACT GCTGAACCCT TACTGGTGTC ATCCCCGGAG
CACCCAAAAC GCTGGGGCCA AGTCAGCGAG GCCTACGTGA GCCTCCTAGA GGAAAAGAAT
GAGGCTCAGA GAGGTGAAGT AAGCTGCTCA GGATTGCACA GCTGGCACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60464
Clone ID Related Accession (Same CDS sequence) XM_011525127.2
Accession Version XM_011525127.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1005bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product N-sulphoglucosamine sulphohydrolase isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011525127.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGAGCTGCC CCGTGCCCGC CTGCTGCGCG CTGCTGCTAG TCCTGGGGCT CTGCCGGGCG 
CGTCCCCGGA ACGCACTGCT GCTCCTCGCG GATGACGGAG GCTTTGAGAG TGGCGCGTAC
AACAACAGCG CCATCGCCAC CCCGCACCTG GACGCCTTGG CCCGCCGCAG CCTCCTCTTT
CGCAATGCCT TCACCTCGGT CAGCAGCTGC TCTCCCAGCC GCGCCAGCCT CCTCACTGGC
CTGCCCCAGC ATCAGAATGG GATGTACGGG CTGCACCAGG ACGTGCACCA CTTCAACTCC
TTCGACAAGG TGCGGAGCCT GCCGCTGCTG CTCAGCCAAG CTGGTGTGCG CACAGGCATC
ATCGGGAAGA AGCACGTGGG GCCGGAGACC GTGTACCCGT TTGACTTTGC GTACACGGAG
GAGAATGGCT CCGTCCTCCA GGTGGGGCGG AACATCACTA GAATTAAGCT GCTCGTCCGG
AAATTCCTGC AGACTCAGGA TGACCGGCCT TTCTTCCTCT ACGTCGCCTT CCACGACCCC
CACCGCTGTG GGCACTCCCA GCCCCAGTAC GGAACCTTCT GTGAGAAGTT TGGCAACGGA
GAGAGCGGCA TGGGTCGTAT CCCAGACTGG ACCCCCCAGG CCTACGACCC ACTGGACGTG
CTGGTGCCTT ACTTCGTCCC CAACACCCCG GCAGCCCGAG CCGACCTGGC CGCTCAGTAC
ACCACCGTCG GCCGCATGGA CCAAGGAGTT GGACTGGTGC TCCAGGAGCT GCGTGACGCC
GGTGTCCTGA ACGACACACT GGTGATCTTC ACGTCCGACA ACGGGATCCC CTTCCCCAGC
GGCAGGACCA ACCTGTACTG GCCGGGCACT GCTGAACCCT TACTGGTGTC ATCCCCGGAG
CACCCAAAAC GCTGGGGCCA AGTCAGCGAG GCCTACGTGA GCCTCCTAGA GGAAAAGAAT
GAGGCTCAGA GAGGTGAAGT AAGCTGCTCA GGATTGCACA GCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011523429.1
CDS21..1025
Misc Feature(1)78..>968
Misc Feature(2)90..>968
Misc Feature(3)order(111..116,228..230,387..389,393..395,561..563,837..842)
Misc Feature(4)order(111..116,228..230,387..389,393..395,561..563,840..842)
Misc Feature(5)order(111..116,228..230,837..842)
Translation

Target ORF information:

RefSeq Version XM_011525127.2
Organism Homo sapiens(Human)
Definition Homo sapiens N-sulfoglucosamine sulfohydrolase (SGSH), transcript variant X15, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011525127.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGAGCTGCC CCGTGCCCGC CTGCTGCGCG CTGCTGCTAG TCCTGGGGCT CTGCCGGGCG 
CGTCCCCGGA ACGCACTGCT GCTCCTCGCG GATGACGGAG GCTTTGAGAG TGGCGCGTAC
AACAACAGCG CCATCGCCAC CCCGCACCTG GACGCCTTGG CCCGCCGCAG CCTCCTCTTT
CGCAATGCCT TCACCTCGGT CAGCAGCTGC TCTCCCAGCC GCGCCAGCCT CCTCACTGGC
CTGCCCCAGC ATCAGAATGG GATGTACGGG CTGCACCAGG ACGTGCACCA CTTCAACTCC
TTCGACAAGG TGCGGAGCCT GCCGCTGCTG CTCAGCCAAG CTGGTGTGCG CACAGGCATC
ATCGGGAAGA AGCACGTGGG GCCGGAGACC GTGTACCCGT TTGACTTTGC GTACACGGAG
GAGAATGGCT CCGTCCTCCA GGTGGGGCGG AACATCACTA GAATTAAGCT GCTCGTCCGG
AAATTCCTGC AGACTCAGGA TGACCGGCCT TTCTTCCTCT ACGTCGCCTT CCACGACCCC
CACCGCTGTG GGCACTCCCA GCCCCAGTAC GGAACCTTCT GTGAGAAGTT TGGCAACGGA
GAGAGCGGCA TGGGTCGTAT CCCAGACTGG ACCCCCCAGG CCTACGACCC ACTGGACGTG
CTGGTGCCTT ACTTCGTCCC CAACACCCCG GCAGCCCGAG CCGACCTGGC CGCTCAGTAC
ACCACCGTCG GCCGCATGGA CCAAGGAGTT GGACTGGTGC TCCAGGAGCT GCGTGACGCC
GGTGTCCTGA ACGACACACT GGTGATCTTC ACGTCCGACA ACGGGATCCC CTTCCCCAGC
GGCAGGACCA ACCTGTACTG GCCGGGCACT GCTGAACCCT TACTGGTGTC ATCCCCGGAG
CACCCAAAAC GCTGGGGCCA AGTCAGCGAG GCCTACGTGA GCCTCCTAGA GGAAAAGAAT
GAGGCTCAGA GAGGTGAAGT AAGCTGCTCA GGATTGCACA GCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu87041
Clone ID Related Accession (Same CDS sequence) XM_017024951.1
Accession Version XM_017024951.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1011bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product N-sulphoglucosamine sulphohydrolase isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGAGCTGCC CCGTGCCCGC CTGCTGCGCG CTGCTGCTAG TCCTGGGGCT CTGCCGGGCG 
CGTCCCCGGA ACGCACTGCT GCTCCTCGCG GATGACGGAG GCTTTGAGAG TGGCGCGTAC
AACAACAGCG CCATCGCCAC CCCGCACCTG GACGCCTTGG CCCGCCGCAG CCTCCTCTTT
CGCAATGCCT TCACCTCGGT CAGCAGCTGC TCTCCCAGCC GCGCCAGCCT CCTCACTGGC
CTGCCCCAGC ATCAGAATGG GATGTACGGG CTGCACCAGG ACGTGCACCA CTTCAACTCC
TTCGACAAGG TGCGGAGCCT GCCGCTGCTG CTCAGCCAAG CTGGTGTGCG CACAGGCATC
ATCGGGAAGA AGCACGTGGG GCCGGAGACC GTGTACCCGT TTGACTTTGC GTACACGGAG
GAGAATGGCT CCGTCCTCCA GGTGGGGCGG AACATCACTA GAATTAAGCT GCTCGTCCGG
AAATTCCTGC AGACTCAGGA TGACCGGCCT TTCTTCCTCT ACGTCGCCTT CCACGACCCC
CACCGCTGTG GGCACTCCCA GCCCCAGTAC GGAACCTTCT GTGAGAAGTT TGGCAACGGA
GAGAGCGGCA TGGGTCGTAT CCCAGACTGG ACCCCCCAGG CCTACGACCC ACTGGACGTG
CTGGTGCCTT ACTTCGTCCC CAACACCCCG GCAGCCCGAG CCGACCTGGC CGCTCAGTAC
ACCACCGTCG GCCGCATGGA CCAAGGAGTT GGACTGGTGC TCCAGGAGCT GCGTGACGCC
GGTGTCCTGA ACGACACACT GGTGATCTTC ACGTCCGACA ACGGGATCCC CTTCCCCAGC
GGCAGGACCA ACCTGTACTG GCCGGGCACT GCTGAACCCT TACTGGTGTC ATCCCCGGAG
CACCCAAAAC GCTGGGGCCA AGTCAGCGAG GCCTACGTGA GCCTCCTAGA GGAAAAGAAT
GAGGCTCAGA GAGGTGAAGT AAGCTGCTCA GGATTGCACA GCTGTGACTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016880440.1
CDS21..1031
Translation

Target ORF information:

RefSeq Version XM_017024951.1
Organism Homo sapiens(Human)
Definition Homo sapiens N-sulfoglucosamine sulfohydrolase (SGSH), transcript variant X12, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017024951.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGAGCTGCC CCGTGCCCGC CTGCTGCGCG CTGCTGCTAG TCCTGGGGCT CTGCCGGGCG 
CGTCCCCGGA ACGCACTGCT GCTCCTCGCG GATGACGGAG GCTTTGAGAG TGGCGCGTAC
AACAACAGCG CCATCGCCAC CCCGCACCTG GACGCCTTGG CCCGCCGCAG CCTCCTCTTT
CGCAATGCCT TCACCTCGGT CAGCAGCTGC TCTCCCAGCC GCGCCAGCCT CCTCACTGGC
CTGCCCCAGC ATCAGAATGG GATGTACGGG CTGCACCAGG ACGTGCACCA CTTCAACTCC
TTCGACAAGG TGCGGAGCCT GCCGCTGCTG CTCAGCCAAG CTGGTGTGCG CACAGGCATC
ATCGGGAAGA AGCACGTGGG GCCGGAGACC GTGTACCCGT TTGACTTTGC GTACACGGAG
GAGAATGGCT CCGTCCTCCA GGTGGGGCGG AACATCACTA GAATTAAGCT GCTCGTCCGG
AAATTCCTGC AGACTCAGGA TGACCGGCCT TTCTTCCTCT ACGTCGCCTT CCACGACCCC
CACCGCTGTG GGCACTCCCA GCCCCAGTAC GGAACCTTCT GTGAGAAGTT TGGCAACGGA
GAGAGCGGCA TGGGTCGTAT CCCAGACTGG ACCCCCCAGG CCTACGACCC ACTGGACGTG
CTGGTGCCTT ACTTCGTCCC CAACACCCCG GCAGCCCGAG CCGACCTGGC CGCTCAGTAC
ACCACCGTCG GCCGCATGGA CCAAGGAGTT GGACTGGTGC TCCAGGAGCT GCGTGACGCC
GGTGTCCTGA ACGACACACT GGTGATCTTC ACGTCCGACA ACGGGATCCC CTTCCCCAGC
GGCAGGACCA ACCTGTACTG GCCGGGCACT GCTGAACCCT TACTGGTGTC ATCCCCGGAG
CACCCAAAAC GCTGGGGCCA AGTCAGCGAG GCCTACGTGA GCCTCCTAGA GGAAAAGAAT
GAGGCTCAGA GAGGTGAAGT AAGCTGCTCA GGATTGCACA GCTGTGACTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu87042
Clone ID Related Accession (Same CDS sequence) XM_017024952.1
Accession Version XM_017024952.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1008bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product N-sulphoglucosamine sulphohydrolase isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGAGCTGCC CCGTGCCCGC CTGCTGCGCG CTGCTGCTAG TCCTGGGGCT CTGCCGGGCG 
CGTCCCCGGA ACGCACTGCT GCTCCTCGCG GATGACGGAG GCTTTGAGAG TGGCGCGTAC
AACAACAGCG CCATCGCCAC CCCGCACCTG GACGCCTTGG CCCGCCGCAG CCTCCTCTTT
CGCAATGCCT TCACCTCGGT CAGCAGCTGC TCTCCCAGCC GCGCCAGCCT CCTCACTGGC
CTGCCCCAGC ATCAGAATGG GATGTACGGG CTGCACCAGG ACGTGCACCA CTTCAACTCC
TTCGACAAGG TGCGGAGCCT GCCGCTGCTG CTCAGCCAAG CTGGTGTGCG CACAGGCATC
ATCGGGAAGA AGCACGTGGG GCCGGAGACC GTGTACCCGT TTGACTTTGC GTACACGGAG
GAGAATGGCT CCGTCCTCCA GGTGGGGCGG AACATCACTA GAATTAAGCT GCTCGTCCGG
AAATTCCTGC AGACTCAGGA TGACCGGCCT TTCTTCCTCT ACGTCGCCTT CCACGACCCC
CACCGCTGTG GGCACTCCCA GCCCCAGTAC GGAACCTTCT GTGAGAAGTT TGGCAACGGA
GAGAGCGGCA TGGGTCGTAT CCCAGACTGG ACCCCCCAGG CCTACGACCC ACTGGACGTG
CTGGTGCCTT ACTTCGTCCC CAACACCCCG GCAGCCCGAG CCGACCTGGC CGCTCAGTAC
ACCACCGTCG GCCGCATGGA CCAAGGAGTT GGACTGGTGC TCCAGGAGCT GCGTGACGCC
GGTGTCCTGA ACGACACACT GGTGATCTTC ACGTCCGACA ACGGGATCCC CTTCCCCAGC
GGCAGGACCA ACCTGTACTG GCCGGGCACT GCTGAACCCT TACTGGTGTC ATCCCCGGAG
CACCCAAAAC GCTGGGGCCA AGTCAGCGAG GCCTACGTGA GCCTCCTAGA GGAAAAGAAT
GAGGCTCAGA GAGGTGAAGT AAGCTGCTCA GGATTGCACA GCTGGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016880441.1
CDS21..1028
Translation

Target ORF information:

RefSeq Version XM_017024952.1
Organism Homo sapiens(Human)
Definition Homo sapiens N-sulfoglucosamine sulfohydrolase (SGSH), transcript variant X14, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017024952.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGAGCTGCC CCGTGCCCGC CTGCTGCGCG CTGCTGCTAG TCCTGGGGCT CTGCCGGGCG 
CGTCCCCGGA ACGCACTGCT GCTCCTCGCG GATGACGGAG GCTTTGAGAG TGGCGCGTAC
AACAACAGCG CCATCGCCAC CCCGCACCTG GACGCCTTGG CCCGCCGCAG CCTCCTCTTT
CGCAATGCCT TCACCTCGGT CAGCAGCTGC TCTCCCAGCC GCGCCAGCCT CCTCACTGGC
CTGCCCCAGC ATCAGAATGG GATGTACGGG CTGCACCAGG ACGTGCACCA CTTCAACTCC
TTCGACAAGG TGCGGAGCCT GCCGCTGCTG CTCAGCCAAG CTGGTGTGCG CACAGGCATC
ATCGGGAAGA AGCACGTGGG GCCGGAGACC GTGTACCCGT TTGACTTTGC GTACACGGAG
GAGAATGGCT CCGTCCTCCA GGTGGGGCGG AACATCACTA GAATTAAGCT GCTCGTCCGG
AAATTCCTGC AGACTCAGGA TGACCGGCCT TTCTTCCTCT ACGTCGCCTT CCACGACCCC
CACCGCTGTG GGCACTCCCA GCCCCAGTAC GGAACCTTCT GTGAGAAGTT TGGCAACGGA
GAGAGCGGCA TGGGTCGTAT CCCAGACTGG ACCCCCCAGG CCTACGACCC ACTGGACGTG
CTGGTGCCTT ACTTCGTCCC CAACACCCCG GCAGCCCGAG CCGACCTGGC CGCTCAGTAC
ACCACCGTCG GCCGCATGGA CCAAGGAGTT GGACTGGTGC TCCAGGAGCT GCGTGACGCC
GGTGTCCTGA ACGACACACT GGTGATCTTC ACGTCCGACA ACGGGATCCC CTTCCCCAGC
GGCAGGACCA ACCTGTACTG GCCGGGCACT GCTGAACCCT TACTGGTGTC ATCCCCGGAG
CACCCAAAAC GCTGGGGCCA AGTCAGCGAG GCCTACGTGA GCCTCCTAGA GGAAAAGAAT
GAGGCTCAGA GAGGTGAAGT AAGCTGCTCA GGATTGCACA GCTGGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Broad functional correction of molecular impairments by systemic delivery of scAAVrh74-hSGSH gene delivery in MPS IIIA mice.
Molecular therapy : the journal of the American Society of Gene Therapy23(4)638-47(2015 Apr)
Duncan FJ,Naughton BJ,Zaraspe K,Murrey DA,Meadows AS,Clark KR,Newsom DE,White P,Fu H,McCarty DM


book

Structure of sulfamidase provides insight into the molecular pathology of mucopolysaccharidosis IIIA.
Acta crystallographica. Section D, Biological crystallography70(Pt 5)1321-35(2014 May)
Sidhu NS,Schreiber K,Pröpper K,Becker S,Usón I,Sheldrick GM,Gärtner J,Krätzner R,Steinfeld R


book

Enzyme replacement reduces neuropathology in MPS IIIA dogs.
Neurobiology of disease43(2)422-34(2011 Aug)
Crawley AC,Marshall N,Beard H,Hassiotis S,Walsh V,King B,Hucker N,Fuller M,Jolly RD,Hopwood JJ,Hemsley KM


book

Residual activity and proteasomal degradation of p.Ser298Pro sulfamidase identified in patients with a mild clinical phenotype of Sanfilippo A syndrome.
American journal of medical genetics. Part A155A(7)1634-9(2011 Jul)
Muschol N,Pohl S,Meyer A,Gal A,Ullrich K,Braulke T


book

New genetic associations detected in a host response study to hepatitis B vaccine.
Genes and immunity11(3)232-8(2010 Apr)
Davila S,Froeling FE,Tan A,Bonnard C,Boland GJ,Snippe H,Hibberd ML,Seielstad M


 
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