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SIX1 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol SIX1
Entrez Gene ID 6495
Full Name SIX homeobox 1
Synonyms BOS3,DFNA23,TIP39
General protein information
Preferred Names

SIX homeobox 1

Names

homeobox protein SIX1
sine oculis homeobox homolog 1

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

14

14q23.1

Summary The protein encoded by this gene is a homeobox protein that is similar to the Drosophila 'sine oculis' gene product. This gene is found in a cluster of related genes on chromosome 14 and is thought to be involved in limb development. Defects in this gene are a cause of autosomal dominant deafness type 23 (DFNA23) and branchiootic syndrome type 3 (BOS3). [provided by RefSeq, Jul 2008].
Disorder MIM:

601205

Disorder Html: Brachiootic syndrome 3, 608389 (3); Deafness, autosomal dominant 23,

mRNA and Protein(s)

mRNA Protein Name
NM_005982.3 NP_005973.1 homeobox protein SIX1
XM_017021602.1 XP_016877091.1 homeobox protein SIX1 isoform X1



Canis lupus familiaris (dog) SIX1 XP_547841.3
Mus musculus (house mouse) Six1 NP_033215.2
Gallus gallus (chicken) SIX1 NP_001038150.1
Danio rerio (zebrafish) six1b NP_996978.1
Pan troglodytes (chimpanzee) SIX1 XP_509988.2
Rattus norvegicus (Norway rat) Six1 NP_446211.1
Xenopus tropicalis (tropical clawed frog) six1 NP_001093693.1
Macaca mulatta (rhesus monkey) SIX1 XP_001096585.1
Homo sapiens (human) SIX1 NP_005973.1
Bos taurus (cattle) SIX1 XP_002691065.3
Drosophila melanogaster (fruit fly) so NP_476733.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP011695 XP_320814.4


Related articles in PubMed

Six1 promotes colorectal cancer growth and metastasis by stimulating angiogenesis and recruiting tumor-associated macrophages.
Xu H, Zhang Y, Peña MM, Pirisi L, Creek KE
Carcinogenesis38(3)281-292(2017 Mar)

MicroRNA-362 is downregulated in cervical cancer and inhibits cell proliferation, migration and invasion by directly targeting SIX1.
Shi C, Zhang Z
Oncology reports37(1)501-509(2017 Jan)

Pro-survival effects by NF-κB, Akt and ERK(1/2) and anti-apoptosis actions by Six1 disrupt apoptotic functions of TRAIL-Dr4/5 pathway in ovarian cancer.
Yang J, Li G, Zhang K
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie841078-1087(2016 Dec)

Association of three single nucleotide polymorphisms at the SIX1-SIX6 locus with primary open angle glaucoma in the Chinese population.
Sang J, Jia L, Zhao B, Wang H, Zhang N, Wang N
Science China. Life sciences59(7)694-9(2016 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studies strongly suggest that Six1 overexpression promotes CRC growth and metastasis and remodels tumor stroma by stimulating angiogenesis and recruiting TAM. MAPK activation may be a pivotal event in Six1-associated tumor progression.
Title: Six1 promotes colorectal cancer growth and metastasis by stimulating angiogenesis and recruiting tumor-associated macrophages.

Restoration of SIX1 was sufficient to abolish proliferation, migration and invasion induced by miR-362 overexpression in cervical cancer cells. The newly identified miR-362/SIX1 pathway provides insight into cervical cancer progression, and may represent a novel therapeutic target.
Title: MicroRNA-362 is downregulated in cervical cancer and inhibits cell proliferation, migration and invasion by directly targeting SIX1.

Pro-survival effects by NF-kappaB, Akt and ERK(1/2) and anti-apoptosis actions by Six1 disrupt apoptotic functions of TRAIL-Dr4/5 pathway in ovarian cancer, which may explain why up-regulated DR4 and DR5 in ovarian cancer are associated with poor prognosis and low survival ratio of the patients.
Title: Pro-survival effects by NF-κB, Akt and ERK(1/2) and anti-apoptosis actions by Six1 disrupt apoptotic functions of TRAIL-Dr4/5 pathway in ovarian cancer.

results suggest that SIX1 is a key proliferation regulator in mouse DFCs and human PDLCs, which provides novel insight into Six family gene function in mammals.
Title: Six1 is required for mouse dental follicle cell and human periodontal ligament-derived cell proliferation.

study replicated the association of POAG with two SNPs at the SIX1-SIX6 locus and demonstrated that SNPs, rs10483727 and rs33912345, are significantly associated with POAG, especially with NTG in patients aged above 40 years
Title: Association of three single nucleotide polymorphisms at the SIX1-SIX6 locus with primary open angle glaucoma in the Chinese population.

The following SIX1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SIX1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu26939 NM_005982.3
Latest version!
Homo sapiens SIX homeobox 1 (SIX1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$154.50-$216.30
$309.00
OHu85081 XM_017021602.1
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Homo sapiens SIX homeobox 1 (SIX1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $69.30
$99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu26939
Clone ID Related Accession (Same CDS sequence) NM_005982.3
Accession Version NM_005982.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 855bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-03
Organism Homo sapiens(Human)
Product homeobox protein SIX1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC008874.2, X91868.1 and AL049874.3. This sequence is a reference standard in the RefSeqGene project. On Apr 29, 2008 this sequence version replaced NM_005982.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC008874.2, X91868.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCGATGC TGCCGTCGTT TGGCTTTACG CAGGAGCAAG TGGCGTGCGT GTGCGAGGTT 
CTGCAGCAAG GCGGAAACCT GGAGCGCCTG GGCAGGTTCC TGTGGTCACT GCCCGCCTGC
GACCACCTGC ACAAGAACGA GAGCGTACTC AAGGCCAAGG CGGTGGTCGC CTTCCACCGC
GGCAACTTCC GTGAGCTCTA CAAGATCCTG GAGAGCCACC AGTTCTCGCC TCACAACCAC
CCCAAACTGC AGCAACTGTG GCTGAAGGCG CATTACGTGG AGGCCGAGAA GCTGCGCGGC
CGACCCCTGG GCGCCGTGGG CAAATATCGG GTGCGCCGAA AATTTCCACT GCCGCGCACC
ATCTGGGACG GCGAGGAGAC CAGCTACTGC TTCAAGGAGA AGTCGAGGGG TGTCCTGCGG
GAGTGGTACG CGCACAATCC CTACCCATCG CCGCGTGAGA AGCGGGAGCT GGCCGAGGCC
ACCGGCCTCA CCACCACCCA GGTCAGCAAC TGGTTTAAGA ACCGGAGGCA AAGAGACCGG
GCCGCGGAGG CCAAGGAAAG GGAGAACACC GAAAACAATA ACTCCTCCTC CAACAAGCAG
AACCAACTCT CTCCTCTGGA AGGGGGCAAG CCGCTCATGT CCAGCTCAGA AGAGGAATTC
TCACCTCCCC AAAGTCCAGA CCAGAACTCG GTCCTTCTGC TGCAGGGCAA TATGGGCCAC
GCCAGGAGCT CAAACTATTC TCTCCCGGGC TTAACAGCCT CGCAGCCCAG TCACGGCCTG
CAGACCCACC AGCATCAGCT CCAAGACTCT CTGCTCGGCC CCCTCACCTC CAGTCTGGTG
GACTTGGGGT CCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005973.1
CDS249..1103
Misc Feature(1)147..149
Misc Feature(2)273..602
Misc Feature(3)633..788
Misc Feature(4)order(636..638,756..758,765..770,777..779)
Exon (1)1..808
Exon (2)809..2687
Translation

Target ORF information:

RefSeq Version NM_005982.3
Organism Homo sapiens(Human)
Definition Homo sapiens SIX homeobox 1 (SIX1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005982.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCGATGC TGCCGTCGTT TGGCTTTACG CAGGAGCAAG TGGCGTGCGT GTGCGAGGTT 
CTGCAGCAAG GCGGAAACCT GGAGCGCCTG GGCAGGTTCC TGTGGTCACT GCCCGCCTGC
GACCACCTGC ACAAGAACGA GAGCGTACTC AAGGCCAAGG CGGTGGTCGC CTTCCACCGC
GGCAACTTCC GTGAGCTCTA CAAGATCCTG GAGAGCCACC AGTTCTCGCC TCACAACCAC
CCCAAACTGC AGCAACTGTG GCTGAAGGCG CATTACGTGG AGGCCGAGAA GCTGCGCGGC
CGACCCCTGG GCGCCGTGGG CAAATATCGG GTGCGCCGAA AATTTCCACT GCCGCGCACC
ATCTGGGACG GCGAGGAGAC CAGCTACTGC TTCAAGGAGA AGTCGAGGGG TGTCCTGCGG
GAGTGGTACG CGCACAATCC CTACCCATCG CCGCGTGAGA AGCGGGAGCT GGCCGAGGCC
ACCGGCCTCA CCACCACCCA GGTCAGCAAC TGGTTTAAGA ACCGGAGGCA AAGAGACCGG
GCCGCGGAGG CCAAGGAAAG GGAGAACACC GAAAACAATA ACTCCTCCTC CAACAAGCAG
AACCAACTCT CTCCTCTGGA AGGGGGCAAG CCGCTCATGT CCAGCTCAGA AGAGGAATTC
TCACCTCCCC AAAGTCCAGA CCAGAACTCG GTCCTTCTGC TGCAGGGCAA TATGGGCCAC
GCCAGGAGCT CAAACTATTC TCTCCCGGGC TTAACAGCCT CGCAGCCCAG TCACGGCCTG
CAGACCCACC AGCATCAGCT CCAAGACTCT CTGCTCGGCC CCCTCACCTC CAGTCTGGTG
GACTTGGGGT CCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu85081
Clone ID Related Accession (Same CDS sequence) XM_017021602.1
Accession Version XM_017021602.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 522bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product homeobox protein SIX1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
ATGTCGATGC TGCCGTCGTT TGGCTTTACG CAGGAGCAAG TGGCGTGCGT GTGCGAGGTT 
CTGCAGCAAG GCGGAAACCT GGAGCGCCTG GGCAGGTTCC TGTGGTCACT GCCCGCCTGC
GACCACCTGC ACAAGAACGA GAGCGTACTC AAGGCCAAGG CGGTGGTCGC CTTCCACCGC
GGCAACTTCC GTGAGCTCTA CAAGATCCTG GAGAGCCACC AGTTCTCGCC TCACAACCAC
CCCAAACTGC AGCAACTGTG GCTGAAGGCG CATTACGTGG AGGCCGAGAA GCTGCGCGGC
CGACCCCTGG GCGCCGTGGG CAAATATCGG GTGCGCCGAA AATTTCCACT GCCGCGCACC
ATCTGGGACG GCGAGGAGAC CAGCTACTGC TTCAAGGAGA AGTCGAGGGG TGTCCTGCGG
GAGTGGTACG CGCACAATCC CTACCCATCG CCGCGTGAGA AGCGGGAGCT GGCCGAGGCC
ACCGGCCTCA CCACCACCCA GGGAGAACAC CGAAAACAAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016877091.1
CDS301..822
Misc Feature(1)325..654
Misc Feature(2)724..>801
Translation

Target ORF information:

RefSeq Version XM_017021602.1
Organism Homo sapiens(Human)
Definition Homo sapiens SIX homeobox 1 (SIX1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017021602.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGTCGATGC TGCCGTCGTT TGGCTTTACG CAGGAGCAAG TGGCGTGCGT GTGCGAGGTT 
CTGCAGCAAG GCGGAAACCT GGAGCGCCTG GGCAGGTTCC TGTGGTCACT GCCCGCCTGC
GACCACCTGC ACAAGAACGA GAGCGTACTC AAGGCCAAGG CGGTGGTCGC CTTCCACCGC
GGCAACTTCC GTGAGCTCTA CAAGATCCTG GAGAGCCACC AGTTCTCGCC TCACAACCAC
CCCAAACTGC AGCAACTGTG GCTGAAGGCG CATTACGTGG AGGCCGAGAA GCTGCGCGGC
CGACCCCTGG GCGCCGTGGG CAAATATCGG GTGCGCCGAA AATTTCCACT GCCGCGCACC
ATCTGGGACG GCGAGGAGAC CAGCTACTGC TTCAAGGAGA AGTCGAGGGG TGTCCTGCGG
GAGTGGTACG CGCACAATCC CTACCCATCG CCGCGTGAGA AGCGGGAGCT GGCCGAGGCC
ACCGGCCTCA CCACCACCCA GGGAGAACAC CGAAAACAAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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