WNK1 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following WNK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WNK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu84074 XM_017019834.1
Latest version!
Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OHu84075 XM_017019835.1
Latest version!
Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OHu84076 XM_017019836.1
Latest version!
Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OHu84077 XM_017019837.1
Latest version!
Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OHu84078 XM_017019838.1
Latest version!
Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OHu84079 XM_017019839.1
Latest version!
Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OHu28095 NM_213655.4
Latest version!
Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $923.30
$1319.00
OHu28140 NM_001184985.1
Latest version!
Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $923.30
$1319.00
OHu28145 NM_014823.3
Latest version!
Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $923.30
$1319.00
OHu28188 NM_018979.4
Latest version!
Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $923.30
$1319.00
OHu46009 XM_006719003.2
Latest version!
Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu71618 XM_011521002.2
Latest version!
Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu71619 XM_011521003.2
Latest version!
Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu71620 XM_011521004.2
Latest version!
Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu71621 XM_011521005.2
Latest version!
Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu71622 XM_011521006.2
Latest version!
Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu71623 XM_011521007.2
Latest version!
Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu71624 XM_011521008.2
Latest version!
Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu71625 XM_011521009.2
Latest version!
Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu71613 XM_011520997.3
Latest version!
Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu71614 XM_011520998.2
Latest version!
Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu71615 XM_011520999.2
Latest version!
Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu71616 XM_011521000.2
Latest version!
Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu71617 XM_011521001.2
Latest version!
Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
View Old Accession Versions >>
OHu71613 XM_011520997.2 Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu28145 NM_014823.2 Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $923.30
$1319.00
OHu28188 NM_018979.3 Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $923.30
$1319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu84074
    Clone ID Related Accession (Same CDS sequence) XM_017019834.1
    Accession Version XM_017019834.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6687bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase WNK1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT
    CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AACCCATACA ACATCCTCAG
    CAGCAGGGAA TACAGCAGAC AGCCCCTCCT CAACAGACAG TGCAGTATTC ACTTTCACAG
    ACATCAACCT CCAGTGAGGC CACTACTGCA CAGCCAGTGA GTCAGCCTCA AGCTCCACAA
    GTCTTGCCTC AAGTATCAGC TGGAAAACAG GGCTTCCCAC CTCGACTGCC ACCACAGTAC
    CCAGGAGATT CAAATATTGC TCCCTCTTCC AACGTGGCTT CTGTTTGCAT CCATTCTACA
    GTCCTATCCC CTCCCATGCC GACAGAAGTA CTGGCTACAC CTGGGTACTT TCCCACAGTG
    GTGCAGCCTT ATGTGGAATC AAATCTTTTA GTTCCTATGG GTGGTGTAGG AGGACAGGTT
    CAAGTGTCCC AGCCAGGAGG GAGTTTAGCA CAAGCCCCCA CTACATCCTC CCAGCAAGCA
    GTTTTGGAGA GTACTCAGGG AGTCTCTCAG GTTGCTCCTG CAGAGCCAGT TGCAGTAGCA
    CAGACCCAAG CTACCCAGCC GACCACTTTG GCTTCCTCTG TAGACAGTGC ACATTCAGAT
    GTTGCTTCAG GTATGAGTGA TGGCAATGAG AACGTCCCAT CTTCCAGTGG AAGGCATGAA
    GGAAGAACTA CAAAACGGCA TTACCGAAAA TCTGTAAGGA GTCGCTCTCG ACATGAAAAA
    ACTTCACGCC CAAAATTAAG AATTTTGAAT GTTTCAAATA AAGGAGACCG AGTAGTAGAA
    TGTCAATTAG AGACTCATAA TAGGAAAATG GTTACATTCA AATTTGACCT AGATGGTGAC
    AACCCCGAGG AGATAGCAAC AATTATGGTG AACAATGACT TTATTCTAGC AATAGAGAGA
    GAGTCGTTTG TGGATCAAGT GCGAGAAATT ATTGAAAAAG CTGATGAAAT GCTCAGTGAG
    GATGTCAGTG TGGAACCAGA GGGTGATCAG GGATTGGAGA GTCTACAAGG AAAGGATGAC
    TATGGCTTTT CAGGTTCTCA GAAATTGGAA GGAGAGTTCA AACAACCAAT TCCTGCGTCT
    TCCATGCCAC AGCAAATAGG CATTCCTACC AGTTCTTTAA CTCAAGTTGT TCATTCTGCG
    GGAAGGCGGT TTATAGTGAG TCCTGTGCCA GAAAGCCGAT TACGAGAATC AAAAGTTTTC
    CCCAGTGAAA TAACAGATAC AGTTGCTGCC TCTACAGCTC AGAGCCCTGG AATGAACTTG
    TCTCACTCTG CATCATCCCT TAGTCTACAA CAGGCCTTTT CTGAACTTAG ACGTGCCCAA
    ATGACAGAAG GACCCAACAC AGCACCTCCA AACTTTAGTC ATACAGGACC AACATTTCCA
    GTAGTACCTC CTTTCTTAAG TAGCATTGCT GGAGTCCCAA CCACAGCAGC AGCCACAGCA
    CCAGTCCCTG CAACAAGCAG CCCTCCTAAT GACATTTCCA CATCAGTAAT TCAGTCTGAG
    GTTACAGTGC CCACTGAAGA GGGGATTGCT GGAGTTGCCA CCAGCACAGG TGTGGTAACT
    TCAGGTGGTC TCCCCATACC ACCTGTGTCT GAATCACCAG TACTTTCCAG CGTAGTTTCA
    AGTATCACAA TACCTGCAGT TGTCTCAATA TCTACTACAT CCCCGTCACT TCAAGTCCCC
    ACATCCACAT CTGAGATCGT TGTTTCTAGT ACAGCACTGT ATCCTTCAGT AACAGTTTCA
    GCAACTTCAG CCTCTGCAGG GGGCAGTACT GCTACCCCAG GTCCTAAGCC TCCAGCTGTA
    GTATCTCAGC AGGCAGCAGG CAGCACTACT GTGGGAGCCA CATTAACATC AGTTTCTACC
    ACCACTTCAT TCCCAAGCAC AGCTTCACAG CTGTGCATTC AGCTTAGCAG CAGTACTTCT
    ACTCCTACTT TAGCTGAAAC CGTGGTAGTT AGCGCACACT CACTAGATAA GACATCTCAT
    AGCAGTACAA CTGGATTGGC TTTCTCCCTC TCTGCACCAT CTTCCTCTTC CTCTCCTGGA
    GCAGGAGTGT CTAGTTATAT TTCTCAGCCT GGTGGGCTGC ATCCTTTGGT CATTCCATCA
    GTGATAGCTT CTACTCCTAT TCTTCCCCAA GCAGCAGGAC CTACTTCTAC ACCTTTATTA
    CCCCAAGTAC CTAGTATCCC ACCCTTGGTA CAGCCTGTTG CCAATGTGCC TGCTGTACAG
    CAGACACTAA TTCATAGTCA GCCTCAACCA GCTTTGCTTC CCAACCAGCC CCATACTCAT
    TGTCCTGAAG TAGATTCTGA TACACAACCC AAAGCTCCTG GAATTGATGA CATAAAGACT
    CTAGAAGAAA AGCTGCGGTC TCTGTTCAGT GAACACAGCT CATCTGGAGC TCAGCATGCC
    TCTGTCTCAC TGGAGACCTC ACTAGTCATA GAGAGCACTG TCACACCAGG CATCCCAACT
    ACTGCTGTTG CACCAAGCAA ACTCCTGACT TCTACCACAA GTACTTGCTT ACCACCAACC
    AATTTACCAC TAGGAACAGT TGCTTTGCCA GTTACACCAG TGGTCACACC TGGGCAAGTT
    TCTACCCCAG TCAGCACTAC TACATCAGGA GTGAAACCTG GAACTGCTCC CTCCAAGCCA
    CCTCTAACTA AGGCTCCGGT GCTGCCAGTG GGTACTGAAC TTCCAGCAGG TACTCTACCC
    AGCGAGCAGC TGCCACCTTT TCCAGGACCT TCTCTAACCC AGTCCCAGCA ACCTCTAGAG
    GATCTTGATG CTCAATTGAG AAGAACACTT AGTCCAGAGA TGATCACAGT GACTTCTGCG
    GTTGGTCCTG TGTCCATGGC GGCTCCAACA GCAATCACAG AAGCAGGAAC ACAGCCTCAG
    AAGGGTGTTT CTCAAGTCAA AGAAGGCCCT GTCCTAGCAA CTAGTTCAGG AGCTGGTGTT
    TTTAAGATGG GACGATTTCA GGTTTCTGTT GCAGCAGACG GTGCCCAGAA AGAGGGTAAA
    AATAAGTCAG AAGATGCAAA GTCTGTTCAT TTTGAATCCA GCACCTCAGA GTCCTCAGTG
    CTATCAAGTA GTAGTCCAGA GAGTACCTTG GTGAAACCAG AGCCGAATGG CATAACCATC
    CCTGGTATCT CTTCAGATGT GCCAGAGAGT GCCCACAAAA CTACTGCCTC AGAGGCAAAG
    TCAGACACTG GGCAGCCTAC CAAGGTTGGA CGTTTTCAGG TGACAACTAC AGCAAACAAA
    GTGGGTCGTT TCTCTGTATC AAAAACTGAG GACAAGATCA CTGACACAAA GAAAGAAGGA
    CCAGTGGCAT CTCCTCCTTT TATGGATTTG GAACAAGCTG TTCTTCCTGC TGTGATACCA
    AAGAAAGAGA AGCCTGAACT GTCAGAGCCT TCACATCTAA ATGGGCCGTC TTCTGACCCG
    GAGGCCGCTT TTTTAAGTAG GGATGTGGAT GATGGTTCCG GTAGTCCACA CTCGCCCCAT
    CAGCTGAGCT CAAAGAGCCT TCCTAGCCAG AATCTAAGTC AAAGCCTTAG TAATTCATTT
    AACTCCTCTT ACATGAGTAG CGACAATGAG TCAGATATCG AAGATGAAGA CTTAAAGTTA
    GAGCTGCGAC GACTACGAGA TAAACATCTC AAAGAGATTC AGGACCTGCA GAGTCGCCAG
    AAGCATGAAA TTGAATCTTT GTATACCAAA CTGGGCAAGG TGCCCCCTGC TGTTATTATT
    CCCCCAGCTG CTCCCCTTTC AGGGAGAAGA CGACGACCCA CTAAAAGCAA AGGCAGCAAA
    TCTAGTCGAA GCAGTTCCTT GGGGAATAAA AGCCCCCAGC TTTCAGGTAA CCTGTCTGGT
    CAGAGTGCAG CTTCAGTCTT GCACCCCCAG CAGACCCTCC ACCCTCCTGG CAACATCCCA
    GAGTCCGGGC AGAATCAGCT GTTACAGCCC CTTAAGCCAT CTCCCTCCAG TGACAACCTC
    TATTCAGCCT TCACCAGTGA TGGTGCCATT TCAGTACCAA GCCTTTCTGC TCCAGGTCAA
    GGAACCAGCA GCACAAACAC TGTTGGGGCA ACAGTGAACA GCCAAGCCGC CCAAGCTCAG
    CCTCCTGCCA TGACGTCCAG CAGGAAGGGC ACATTCACAG ATGACTTGCA CAAGTTGGTA
    GACAATTGGG CCCGAGATGC CATGAATCTC TCAGGCAGGA GAGGAAGCAA AGGGCACATG
    AATTACGAGG GCCCTGGAAT GGCAAGGAAG TTCTCTGCAC CTGGGCAACT GTGCATCTCC
    ATGACCTCGA ACCTGGGTGG CTCTGCCCCC ATCTCTGCAG CATCAGCTAC CTCTCTAGGT
    CACTTCACCA AGTCTATGTG CCCCCCACAG CAGTATGGCT TTCCAGCTAC CCCATTTGGC
    GCTCAATGGA GTGGGACGGG TGGCCCAGCA CCACAGCCAC TTGGCCAGTT CCAACCTGTG
    GGAACTGCCT CCTTGCAGAA TTTCAACATC AGCAATTTGC AGAAATCCAT CAGCAACCCC
    CCAGGCTCCA ACCTGCGGAC CACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016875323.1
    CDS969..7655
    Translation

    Target ORF information:

    RefSeq Version XM_017019834.1
    Organism Homo sapiens(human)
    Definition Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017019834.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT
    CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AACCCATACA ACATCCTCAG
    CAGCAGGGAA TACAGCAGAC AGCCCCTCCT CAACAGACAG TGCAGTATTC ACTTTCACAG
    ACATCAACCT CCAGTGAGGC CACTACTGCA CAGCCAGTGA GTCAGCCTCA AGCTCCACAA
    GTCTTGCCTC AAGTATCAGC TGGAAAACAG GGCTTCCCAC CTCGACTGCC ACCACAGTAC
    CCAGGAGATT CAAATATTGC TCCCTCTTCC AACGTGGCTT CTGTTTGCAT CCATTCTACA
    GTCCTATCCC CTCCCATGCC GACAGAAGTA CTGGCTACAC CTGGGTACTT TCCCACAGTG
    GTGCAGCCTT ATGTGGAATC AAATCTTTTA GTTCCTATGG GTGGTGTAGG AGGACAGGTT
    CAAGTGTCCC AGCCAGGAGG GAGTTTAGCA CAAGCCCCCA CTACATCCTC CCAGCAAGCA
    GTTTTGGAGA GTACTCAGGG AGTCTCTCAG GTTGCTCCTG CAGAGCCAGT TGCAGTAGCA
    CAGACCCAAG CTACCCAGCC GACCACTTTG GCTTCCTCTG TAGACAGTGC ACATTCAGAT
    GTTGCTTCAG GTATGAGTGA TGGCAATGAG AACGTCCCAT CTTCCAGTGG AAGGCATGAA
    GGAAGAACTA CAAAACGGCA TTACCGAAAA TCTGTAAGGA GTCGCTCTCG ACATGAAAAA
    ACTTCACGCC CAAAATTAAG AATTTTGAAT GTTTCAAATA AAGGAGACCG AGTAGTAGAA
    TGTCAATTAG AGACTCATAA TAGGAAAATG GTTACATTCA AATTTGACCT AGATGGTGAC
    AACCCCGAGG AGATAGCAAC AATTATGGTG AACAATGACT TTATTCTAGC AATAGAGAGA
    GAGTCGTTTG TGGATCAAGT GCGAGAAATT ATTGAAAAAG CTGATGAAAT GCTCAGTGAG
    GATGTCAGTG TGGAACCAGA GGGTGATCAG GGATTGGAGA GTCTACAAGG AAAGGATGAC
    TATGGCTTTT CAGGTTCTCA GAAATTGGAA GGAGAGTTCA AACAACCAAT TCCTGCGTCT
    TCCATGCCAC AGCAAATAGG CATTCCTACC AGTTCTTTAA CTCAAGTTGT TCATTCTGCG
    GGAAGGCGGT TTATAGTGAG TCCTGTGCCA GAAAGCCGAT TACGAGAATC AAAAGTTTTC
    CCCAGTGAAA TAACAGATAC AGTTGCTGCC TCTACAGCTC AGAGCCCTGG AATGAACTTG
    TCTCACTCTG CATCATCCCT TAGTCTACAA CAGGCCTTTT CTGAACTTAG ACGTGCCCAA
    ATGACAGAAG GACCCAACAC AGCACCTCCA AACTTTAGTC ATACAGGACC AACATTTCCA
    GTAGTACCTC CTTTCTTAAG TAGCATTGCT GGAGTCCCAA CCACAGCAGC AGCCACAGCA
    CCAGTCCCTG CAACAAGCAG CCCTCCTAAT GACATTTCCA CATCAGTAAT TCAGTCTGAG
    GTTACAGTGC CCACTGAAGA GGGGATTGCT GGAGTTGCCA CCAGCACAGG TGTGGTAACT
    TCAGGTGGTC TCCCCATACC ACCTGTGTCT GAATCACCAG TACTTTCCAG CGTAGTTTCA
    AGTATCACAA TACCTGCAGT TGTCTCAATA TCTACTACAT CCCCGTCACT TCAAGTCCCC
    ACATCCACAT CTGAGATCGT TGTTTCTAGT ACAGCACTGT ATCCTTCAGT AACAGTTTCA
    GCAACTTCAG CCTCTGCAGG GGGCAGTACT GCTACCCCAG GTCCTAAGCC TCCAGCTGTA
    GTATCTCAGC AGGCAGCAGG CAGCACTACT GTGGGAGCCA CATTAACATC AGTTTCTACC
    ACCACTTCAT TCCCAAGCAC AGCTTCACAG CTGTGCATTC AGCTTAGCAG CAGTACTTCT
    ACTCCTACTT TAGCTGAAAC CGTGGTAGTT AGCGCACACT CACTAGATAA GACATCTCAT
    AGCAGTACAA CTGGATTGGC TTTCTCCCTC TCTGCACCAT CTTCCTCTTC CTCTCCTGGA
    GCAGGAGTGT CTAGTTATAT TTCTCAGCCT GGTGGGCTGC ATCCTTTGGT CATTCCATCA
    GTGATAGCTT CTACTCCTAT TCTTCCCCAA GCAGCAGGAC CTACTTCTAC ACCTTTATTA
    CCCCAAGTAC CTAGTATCCC ACCCTTGGTA CAGCCTGTTG CCAATGTGCC TGCTGTACAG
    CAGACACTAA TTCATAGTCA GCCTCAACCA GCTTTGCTTC CCAACCAGCC CCATACTCAT
    TGTCCTGAAG TAGATTCTGA TACACAACCC AAAGCTCCTG GAATTGATGA CATAAAGACT
    CTAGAAGAAA AGCTGCGGTC TCTGTTCAGT GAACACAGCT CATCTGGAGC TCAGCATGCC
    TCTGTCTCAC TGGAGACCTC ACTAGTCATA GAGAGCACTG TCACACCAGG CATCCCAACT
    ACTGCTGTTG CACCAAGCAA ACTCCTGACT TCTACCACAA GTACTTGCTT ACCACCAACC
    AATTTACCAC TAGGAACAGT TGCTTTGCCA GTTACACCAG TGGTCACACC TGGGCAAGTT
    TCTACCCCAG TCAGCACTAC TACATCAGGA GTGAAACCTG GAACTGCTCC CTCCAAGCCA
    CCTCTAACTA AGGCTCCGGT GCTGCCAGTG GGTACTGAAC TTCCAGCAGG TACTCTACCC
    AGCGAGCAGC TGCCACCTTT TCCAGGACCT TCTCTAACCC AGTCCCAGCA ACCTCTAGAG
    GATCTTGATG CTCAATTGAG AAGAACACTT AGTCCAGAGA TGATCACAGT GACTTCTGCG
    GTTGGTCCTG TGTCCATGGC GGCTCCAACA GCAATCACAG AAGCAGGAAC ACAGCCTCAG
    AAGGGTGTTT CTCAAGTCAA AGAAGGCCCT GTCCTAGCAA CTAGTTCAGG AGCTGGTGTT
    TTTAAGATGG GACGATTTCA GGTTTCTGTT GCAGCAGACG GTGCCCAGAA AGAGGGTAAA
    AATAAGTCAG AAGATGCAAA GTCTGTTCAT TTTGAATCCA GCACCTCAGA GTCCTCAGTG
    CTATCAAGTA GTAGTCCAGA GAGTACCTTG GTGAAACCAG AGCCGAATGG CATAACCATC
    CCTGGTATCT CTTCAGATGT GCCAGAGAGT GCCCACAAAA CTACTGCCTC AGAGGCAAAG
    TCAGACACTG GGCAGCCTAC CAAGGTTGGA CGTTTTCAGG TGACAACTAC AGCAAACAAA
    GTGGGTCGTT TCTCTGTATC AAAAACTGAG GACAAGATCA CTGACACAAA GAAAGAAGGA
    CCAGTGGCAT CTCCTCCTTT TATGGATTTG GAACAAGCTG TTCTTCCTGC TGTGATACCA
    AAGAAAGAGA AGCCTGAACT GTCAGAGCCT TCACATCTAA ATGGGCCGTC TTCTGACCCG
    GAGGCCGCTT TTTTAAGTAG GGATGTGGAT GATGGTTCCG GTAGTCCACA CTCGCCCCAT
    CAGCTGAGCT CAAAGAGCCT TCCTAGCCAG AATCTAAGTC AAAGCCTTAG TAATTCATTT
    AACTCCTCTT ACATGAGTAG CGACAATGAG TCAGATATCG AAGATGAAGA CTTAAAGTTA
    GAGCTGCGAC GACTACGAGA TAAACATCTC AAAGAGATTC AGGACCTGCA GAGTCGCCAG
    AAGCATGAAA TTGAATCTTT GTATACCAAA CTGGGCAAGG TGCCCCCTGC TGTTATTATT
    CCCCCAGCTG CTCCCCTTTC AGGGAGAAGA CGACGACCCA CTAAAAGCAA AGGCAGCAAA
    TCTAGTCGAA GCAGTTCCTT GGGGAATAAA AGCCCCCAGC TTTCAGGTAA CCTGTCTGGT
    CAGAGTGCAG CTTCAGTCTT GCACCCCCAG CAGACCCTCC ACCCTCCTGG CAACATCCCA
    GAGTCCGGGC AGAATCAGCT GTTACAGCCC CTTAAGCCAT CTCCCTCCAG TGACAACCTC
    TATTCAGCCT TCACCAGTGA TGGTGCCATT TCAGTACCAA GCCTTTCTGC TCCAGGTCAA
    GGAACCAGCA GCACAAACAC TGTTGGGGCA ACAGTGAACA GCCAAGCCGC CCAAGCTCAG
    CCTCCTGCCA TGACGTCCAG CAGGAAGGGC ACATTCACAG ATGACTTGCA CAAGTTGGTA
    GACAATTGGG CCCGAGATGC CATGAATCTC TCAGGCAGGA GAGGAAGCAA AGGGCACATG
    AATTACGAGG GCCCTGGAAT GGCAAGGAAG TTCTCTGCAC CTGGGCAACT GTGCATCTCC
    ATGACCTCGA ACCTGGGTGG CTCTGCCCCC ATCTCTGCAG CATCAGCTAC CTCTCTAGGT
    CACTTCACCA AGTCTATGTG CCCCCCACAG CAGTATGGCT TTCCAGCTAC CCCATTTGGC
    GCTCAATGGA GTGGGACGGG TGGCCCAGCA CCACAGCCAC TTGGCCAGTT CCAACCTGTG
    GGAACTGCCT CCTTGCAGAA TTTCAACATC AGCAATTTGC AGAAATCCAT CAGCAACCCC
    CCAGGCTCCA ACCTGCGGAC CACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu84075
    Clone ID Related Accession (Same CDS sequence) XM_017019835.1
    Accession Version XM_017019835.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6606bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase WNK1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGCAGGGAAT ACAGCAGACA
    GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC
    ACTACTGCAC AGCCAGTGAG TCAGCCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT
    GGAAAACAGG GCTTCCCACC TCGACTGCCA CCACAGTACC CAGGAGATTC AAATATTGCT
    CCCTCTTCCA ACGTGGCTTC TGTTTGCATC CATTCTACAG TCCTATCCCC TCCCATGCCG
    ACAGAAGTAC TGGCTACACC TGGGTACTTT CCCACAGTGG TGCAGCCTTA TGTGGAATCA
    AATCTTTTAG TTCCTATGGG TGGTGTAGGA GGACAGGTTC AAGTGTCCCA GCCAGGAGGG
    AGTTTAGCAC AAGCCCCCAC TACATCCTCC CAGCAAGCAG TTTTGGAGAG TACTCAGGGA
    GTCTCTCAGG TTGCTCCTGC AGAGCCAGTT GCAGTAGCAC AGACCCAAGC TACCCAGCCG
    ACCACTTTGG CTTCCTCTGT AGACAGTGCA CATTCAGATG TTGCTTCAGG TATGAGTGAT
    GGCAATGAGA ACGTCCCATC TTCCAGTGGA AGGCATGAAG GAAGAACTAC AAAACGGCAT
    TACCGAAAAT CTGTAAGGAG TCGCTCTCGA CATGAAAAAA CTTCACGCCC AAAATTAAGA
    ATTTTGAATG TTTCAAATAA AGGAGACCGA GTAGTAGAAT GTCAATTAGA GACTCATAAT
    AGGAAAATGG TTACATTCAA ATTTGACCTA GATGGTGACA ACCCCGAGGA GATAGCAACA
    ATTATGGTGA ACAATGACTT TATTCTAGCA ATAGAGAGAG AGTCGTTTGT GGATCAAGTG
    CGAGAAATTA TTGAAAAAGC TGATGAAATG CTCAGTGAGG ATGTCAGTGT GGAACCAGAG
    GGTGATCAGG GATTGGAGAG TCTACAAGGA AAGGATGACT ATGGCTTTTC AGGTTCTCAG
    AAATTGGAAG GAGAGTTCAA ACAACCAATT CCTGCGTCTT CCATGCCACA GCAAATAGGC
    ATTCCTACCA GTTCTTTAAC TCAAGTTGTT CATTCTGCGG GAAGGCGGTT TATAGTGAGT
    CCTGTGCCAG AAAGCCGATT ACGAGAATCA AAAGTTTTCC CCAGTGAAAT AACAGATACA
    GTTGCTGCCT CTACAGCTCA GAGCCCTGGA ATGAACTTGT CTCACTCTGC ATCATCCCTT
    AGTCTACAAC AGGCCTTTTC TGAACTTAGA CGTGCCCAAA TGACAGAAGG ACCCAACACA
    GCACCTCCAA ACTTTAGTCA TACAGGACCA ACATTTCCAG TAGTACCTCC TTTCTTAAGT
    AGCATTGCTG GAGTCCCAAC CACAGCAGCA GCCACAGCAC CAGTCCCTGC AACAAGCAGC
    CCTCCTAATG ACATTTCCAC ATCAGTAATT CAGTCTGAGG TTACAGTGCC CACTGAAGAG
    GGGATTGCTG GAGTTGCCAC CAGCACAGGT GTGGTAACTT CAGGTGGTCT CCCCATACCA
    CCTGTGTCTG AATCACCAGT ACTTTCCAGC GTAGTTTCAA GTATCACAAT ACCTGCAGTT
    GTCTCAATAT CTACTACATC CCCGTCACTT CAAGTCCCCA CATCCACATC TGAGATCGTT
    GTTTCTAGTA CAGCACTGTA TCCTTCAGTA ACAGTTTCAG CAACTTCAGC CTCTGCAGGG
    GGCAGTACTG CTACCCCAGG TCCTAAGCCT CCAGCTGTAG TATCTCAGCA GGCAGCAGGC
    AGCACTACTG TGGGAGCCAC ATTAACATCA GTTTCTACCA CCACTTCATT CCCAAGCACA
    GCTTCACAGC TGTGCATTCA GCTTAGCAGC AGTACTTCTA CTCCTACTTT AGCTGAAACC
    GTGGTAGTTA GCGCACACTC ACTAGATAAG ACATCTCATA GCAGTACAAC TGGATTGGCT
    TTCTCCCTCT CTGCACCATC TTCCTCTTCC TCTCCTGGAG CAGGAGTGTC TAGTTATATT
    TCTCAGCCTG GTGGGCTGCA TCCTTTGGTC ATTCCATCAG TGATAGCTTC TACTCCTATT
    CTTCCCCAAG CAGCAGGACC TACTTCTACA CCTTTATTAC CCCAAGTACC TAGTATCCCA
    CCCTTGGTAC AGCCTGTTGC CAATGTGCCT GCTGTACAGC AGACACTAAT TCATAGTCAG
    CCTCAACCAG CTTTGCTTCC CAACCAGCCC CATACTCATT GTCCTGAAGT AGATTCTGAT
    ACACAACCCA AAGCTCCTGG AATTGATGAC ATAAAGACTC TAGAAGAAAA GCTGCGGTCT
    CTGTTCAGTG AACACAGCTC ATCTGGAGCT CAGCATGCCT CTGTCTCACT GGAGACCTCA
    CTAGTCATAG AGAGCACTGT CACACCAGGC ATCCCAACTA CTGCTGTTGC ACCAAGCAAA
    CTCCTGACTT CTACCACAAG TACTTGCTTA CCACCAACCA ATTTACCACT AGGAACAGTT
    GCTTTGCCAG TTACACCAGT GGTCACACCT GGGCAAGTTT CTACCCCAGT CAGCACTACT
    ACATCAGGAG TGAAACCTGG AACTGCTCCC TCCAAGCCAC CTCTAACTAA GGCTCCGGTG
    CTGCCAGTGG GTACTGAACT TCCAGCAGGT ACTCTACCCA GCGAGCAGCT GCCACCTTTT
    CCAGGACCTT CTCTAACCCA GTCCCAGCAA CCTCTAGAGG ATCTTGATGC TCAATTGAGA
    AGAACACTTA GTCCAGAGAT GATCACAGTG ACTTCTGCGG TTGGTCCTGT GTCCATGGCG
    GCTCCAACAG CAATCACAGA AGCAGGAACA CAGCCTCAGA AGGGTGTTTC TCAAGTCAAA
    GAAGGCCCTG TCCTAGCAAC TAGTTCAGGA GCTGGTGTTT TTAAGATGGG ACGATTTCAG
    GTTTCTGTTG CAGCAGACGG TGCCCAGAAA GAGGGTAAAA ATAAGTCAGA AGATGCAAAG
    TCTGTTCATT TTGAATCCAG CACCTCAGAG TCCTCAGTGC TATCAAGTAG TAGTCCAGAG
    AGTACCTTGG TGAAACCAGA GCCGAATGGC ATAACCATCC CTGGTATCTC TTCAGATGTG
    CCAGAGAGTG CCCACAAAAC TACTGCCTCA GAGGCAAAGT CAGACACTGG GCAGCCTACC
    AAGGTTGGAC GTTTTCAGGT GACAACTACA GCAAACAAAG TGGGTCGTTT CTCTGTATCA
    AAAACTGAGG ACAAGATCAC TGACACAAAG AAAGAAGGAC CAGTGGCATC TCCTCCTTTT
    ATGGATTTGG AACAAGCTGT TCTTCCTGCT GTGATACCAA AGAAAGAGAA GCCTGAACTG
    TCAGAGCCTT CACATCTAAA TGGGCCGTCT TCTGACCCGG AGGCCGCTTT TTTAAGTAGG
    GATGTGGATG ATGGTTCCGG TAGTCCACAC TCGCCCCATC AGCTGAGCTC AAAGAGCCTT
    CCTAGCCAGA ATCTAAGTCA AAGCCTTAGT AATTCATTTA ACTCCTCTTA CATGAGTAGC
    GACAATGAGT CAGATATCGA AGATGAAGAC TTAAAGTTAG AGCTGCGACG ACTACGAGAT
    AAACATCTCA AAGAGATTCA GGACCTGCAG AGTCGCCAGA AGCATGAAAT TGAATCTTTG
    TATACCAAAC TGGGCAAGGT GCCCCCTGCT GTTATTATTC CCCCAGCTGC TCCCCTTTCA
    GGGAGAAGAC GACGACCCAC TAAAAGCAAA GGCAGCAAAT CTAGTCGAAG CAGTTCCTTG
    GGGAATAAAA GCCCCCAGCT TTCAGGTAAC CTGTCTGGTC AGAGTGCAGC TTCAGTCTTG
    CACCCCCAGC AGACCCTCCA CCCTCCTGGC AACATCCCAG AGTCCGGGCA GAATCAGCTG
    TTACAGCCCC TTAAGCCATC TCCCTCCAGT GACAACCTCT ATTCAGCCTT CACCAGTGAT
    GGTGCCATTT CAGTACCAAG CCTTTCTGCT CCAGGTCAAG GAACCAGCAG CACAAACACT
    GTTGGGGCAA CAGTGAACAG CCAAGCCGCC CAAGCTCAGC CTCCTGCCAT GACGTCCAGC
    AGGAAGGGCA CATTCACAGA TGACTTGCAC AAGTTGGTAG ACAATTGGGC CCGAGATGCC
    ATGAATCTCT CAGGCAGGAG AGGAAGCAAA GGGCACATGA ATTACGAGGG CCCTGGAATG
    GCAAGGAAGT TCTCTGCACC TGGGCAACTG TGCATCTCCA TGACCTCGAA CCTGGGTGGC
    TCTGCCCCCA TCTCTGCAGC ATCAGCTACC TCTCTAGGTC ACTTCACCAA GTCTATGTGC
    CCCCCACAGC AGTATGGCTT TCCAGCTACC CCATTTGGCG CTCAATGGAG TGGGACGGGT
    GGCCCAGCAC CACAGCCACT TGGCCAGTTC CAACCTGTGG GAACTGCCTC CTTGCAGAAT
    TTCAACATCA GCAATTTGCA GAAATCCATC AGCAACCCCC CAGGCTCCAA CCTGCGGACC
    ACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016875324.1
    CDS969..7574
    Translation

    Target ORF information:

    RefSeq Version XM_017019835.1
    Organism Homo sapiens(human)
    Definition Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017019835.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGCAGGGAAT ACAGCAGACA
    GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC
    ACTACTGCAC AGCCAGTGAG TCAGCCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT
    GGAAAACAGG GCTTCCCACC TCGACTGCCA CCACAGTACC CAGGAGATTC AAATATTGCT
    CCCTCTTCCA ACGTGGCTTC TGTTTGCATC CATTCTACAG TCCTATCCCC TCCCATGCCG
    ACAGAAGTAC TGGCTACACC TGGGTACTTT CCCACAGTGG TGCAGCCTTA TGTGGAATCA
    AATCTTTTAG TTCCTATGGG TGGTGTAGGA GGACAGGTTC AAGTGTCCCA GCCAGGAGGG
    AGTTTAGCAC AAGCCCCCAC TACATCCTCC CAGCAAGCAG TTTTGGAGAG TACTCAGGGA
    GTCTCTCAGG TTGCTCCTGC AGAGCCAGTT GCAGTAGCAC AGACCCAAGC TACCCAGCCG
    ACCACTTTGG CTTCCTCTGT AGACAGTGCA CATTCAGATG TTGCTTCAGG TATGAGTGAT
    GGCAATGAGA ACGTCCCATC TTCCAGTGGA AGGCATGAAG GAAGAACTAC AAAACGGCAT
    TACCGAAAAT CTGTAAGGAG TCGCTCTCGA CATGAAAAAA CTTCACGCCC AAAATTAAGA
    ATTTTGAATG TTTCAAATAA AGGAGACCGA GTAGTAGAAT GTCAATTAGA GACTCATAAT
    AGGAAAATGG TTACATTCAA ATTTGACCTA GATGGTGACA ACCCCGAGGA GATAGCAACA
    ATTATGGTGA ACAATGACTT TATTCTAGCA ATAGAGAGAG AGTCGTTTGT GGATCAAGTG
    CGAGAAATTA TTGAAAAAGC TGATGAAATG CTCAGTGAGG ATGTCAGTGT GGAACCAGAG
    GGTGATCAGG GATTGGAGAG TCTACAAGGA AAGGATGACT ATGGCTTTTC AGGTTCTCAG
    AAATTGGAAG GAGAGTTCAA ACAACCAATT CCTGCGTCTT CCATGCCACA GCAAATAGGC
    ATTCCTACCA GTTCTTTAAC TCAAGTTGTT CATTCTGCGG GAAGGCGGTT TATAGTGAGT
    CCTGTGCCAG AAAGCCGATT ACGAGAATCA AAAGTTTTCC CCAGTGAAAT AACAGATACA
    GTTGCTGCCT CTACAGCTCA GAGCCCTGGA ATGAACTTGT CTCACTCTGC ATCATCCCTT
    AGTCTACAAC AGGCCTTTTC TGAACTTAGA CGTGCCCAAA TGACAGAAGG ACCCAACACA
    GCACCTCCAA ACTTTAGTCA TACAGGACCA ACATTTCCAG TAGTACCTCC TTTCTTAAGT
    AGCATTGCTG GAGTCCCAAC CACAGCAGCA GCCACAGCAC CAGTCCCTGC AACAAGCAGC
    CCTCCTAATG ACATTTCCAC ATCAGTAATT CAGTCTGAGG TTACAGTGCC CACTGAAGAG
    GGGATTGCTG GAGTTGCCAC CAGCACAGGT GTGGTAACTT CAGGTGGTCT CCCCATACCA
    CCTGTGTCTG AATCACCAGT ACTTTCCAGC GTAGTTTCAA GTATCACAAT ACCTGCAGTT
    GTCTCAATAT CTACTACATC CCCGTCACTT CAAGTCCCCA CATCCACATC TGAGATCGTT
    GTTTCTAGTA CAGCACTGTA TCCTTCAGTA ACAGTTTCAG CAACTTCAGC CTCTGCAGGG
    GGCAGTACTG CTACCCCAGG TCCTAAGCCT CCAGCTGTAG TATCTCAGCA GGCAGCAGGC
    AGCACTACTG TGGGAGCCAC ATTAACATCA GTTTCTACCA CCACTTCATT CCCAAGCACA
    GCTTCACAGC TGTGCATTCA GCTTAGCAGC AGTACTTCTA CTCCTACTTT AGCTGAAACC
    GTGGTAGTTA GCGCACACTC ACTAGATAAG ACATCTCATA GCAGTACAAC TGGATTGGCT
    TTCTCCCTCT CTGCACCATC TTCCTCTTCC TCTCCTGGAG CAGGAGTGTC TAGTTATATT
    TCTCAGCCTG GTGGGCTGCA TCCTTTGGTC ATTCCATCAG TGATAGCTTC TACTCCTATT
    CTTCCCCAAG CAGCAGGACC TACTTCTACA CCTTTATTAC CCCAAGTACC TAGTATCCCA
    CCCTTGGTAC AGCCTGTTGC CAATGTGCCT GCTGTACAGC AGACACTAAT TCATAGTCAG
    CCTCAACCAG CTTTGCTTCC CAACCAGCCC CATACTCATT GTCCTGAAGT AGATTCTGAT
    ACACAACCCA AAGCTCCTGG AATTGATGAC ATAAAGACTC TAGAAGAAAA GCTGCGGTCT
    CTGTTCAGTG AACACAGCTC ATCTGGAGCT CAGCATGCCT CTGTCTCACT GGAGACCTCA
    CTAGTCATAG AGAGCACTGT CACACCAGGC ATCCCAACTA CTGCTGTTGC ACCAAGCAAA
    CTCCTGACTT CTACCACAAG TACTTGCTTA CCACCAACCA ATTTACCACT AGGAACAGTT
    GCTTTGCCAG TTACACCAGT GGTCACACCT GGGCAAGTTT CTACCCCAGT CAGCACTACT
    ACATCAGGAG TGAAACCTGG AACTGCTCCC TCCAAGCCAC CTCTAACTAA GGCTCCGGTG
    CTGCCAGTGG GTACTGAACT TCCAGCAGGT ACTCTACCCA GCGAGCAGCT GCCACCTTTT
    CCAGGACCTT CTCTAACCCA GTCCCAGCAA CCTCTAGAGG ATCTTGATGC TCAATTGAGA
    AGAACACTTA GTCCAGAGAT GATCACAGTG ACTTCTGCGG TTGGTCCTGT GTCCATGGCG
    GCTCCAACAG CAATCACAGA AGCAGGAACA CAGCCTCAGA AGGGTGTTTC TCAAGTCAAA
    GAAGGCCCTG TCCTAGCAAC TAGTTCAGGA GCTGGTGTTT TTAAGATGGG ACGATTTCAG
    GTTTCTGTTG CAGCAGACGG TGCCCAGAAA GAGGGTAAAA ATAAGTCAGA AGATGCAAAG
    TCTGTTCATT TTGAATCCAG CACCTCAGAG TCCTCAGTGC TATCAAGTAG TAGTCCAGAG
    AGTACCTTGG TGAAACCAGA GCCGAATGGC ATAACCATCC CTGGTATCTC TTCAGATGTG
    CCAGAGAGTG CCCACAAAAC TACTGCCTCA GAGGCAAAGT CAGACACTGG GCAGCCTACC
    AAGGTTGGAC GTTTTCAGGT GACAACTACA GCAAACAAAG TGGGTCGTTT CTCTGTATCA
    AAAACTGAGG ACAAGATCAC TGACACAAAG AAAGAAGGAC CAGTGGCATC TCCTCCTTTT
    ATGGATTTGG AACAAGCTGT TCTTCCTGCT GTGATACCAA AGAAAGAGAA GCCTGAACTG
    TCAGAGCCTT CACATCTAAA TGGGCCGTCT TCTGACCCGG AGGCCGCTTT TTTAAGTAGG
    GATGTGGATG ATGGTTCCGG TAGTCCACAC TCGCCCCATC AGCTGAGCTC AAAGAGCCTT
    CCTAGCCAGA ATCTAAGTCA AAGCCTTAGT AATTCATTTA ACTCCTCTTA CATGAGTAGC
    GACAATGAGT CAGATATCGA AGATGAAGAC TTAAAGTTAG AGCTGCGACG ACTACGAGAT
    AAACATCTCA AAGAGATTCA GGACCTGCAG AGTCGCCAGA AGCATGAAAT TGAATCTTTG
    TATACCAAAC TGGGCAAGGT GCCCCCTGCT GTTATTATTC CCCCAGCTGC TCCCCTTTCA
    GGGAGAAGAC GACGACCCAC TAAAAGCAAA GGCAGCAAAT CTAGTCGAAG CAGTTCCTTG
    GGGAATAAAA GCCCCCAGCT TTCAGGTAAC CTGTCTGGTC AGAGTGCAGC TTCAGTCTTG
    CACCCCCAGC AGACCCTCCA CCCTCCTGGC AACATCCCAG AGTCCGGGCA GAATCAGCTG
    TTACAGCCCC TTAAGCCATC TCCCTCCAGT GACAACCTCT ATTCAGCCTT CACCAGTGAT
    GGTGCCATTT CAGTACCAAG CCTTTCTGCT CCAGGTCAAG GAACCAGCAG CACAAACACT
    GTTGGGGCAA CAGTGAACAG CCAAGCCGCC CAAGCTCAGC CTCCTGCCAT GACGTCCAGC
    AGGAAGGGCA CATTCACAGA TGACTTGCAC AAGTTGGTAG ACAATTGGGC CCGAGATGCC
    ATGAATCTCT CAGGCAGGAG AGGAAGCAAA GGGCACATGA ATTACGAGGG CCCTGGAATG
    GCAAGGAAGT TCTCTGCACC TGGGCAACTG TGCATCTCCA TGACCTCGAA CCTGGGTGGC
    TCTGCCCCCA TCTCTGCAGC ATCAGCTACC TCTCTAGGTC ACTTCACCAA GTCTATGTGC
    CCCCCACAGC AGTATGGCTT TCCAGCTACC CCATTTGGCG CTCAATGGAG TGGGACGGGT
    GGCCCAGCAC CACAGCCACT TGGCCAGTTC CAACCTGTGG GAACTGCCTC CTTGCAGAAT
    TTCAACATCA GCAATTTGCA GAAATCCATC AGCAACCCCC CAGGCTCCAA CCTGCGGACC
    ACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu84076
    Clone ID Related Accession (Same CDS sequence) XM_017019836.1
    Accession Version XM_017019836.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6603bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase WNK1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGGGAATACA GCAGACAGCC
    CCTCCTCAAC AGACAGTGCA GTATTCACTT TCACAGACAT CAACCTCCAG TGAGGCCACT
    ACTGCACAGC CAGTGAGTCA GCCTCAAGCT CCACAAGTCT TGCCTCAAGT ATCAGCTGGA
    AAACAGGGCT TCCCACCTCG ACTGCCACCA CAGTACCCAG GAGATTCAAA TATTGCTCCC
    TCTTCCAACG TGGCTTCTGT TTGCATCCAT TCTACAGTCC TATCCCCTCC CATGCCGACA
    GAAGTACTGG CTACACCTGG GTACTTTCCC ACAGTGGTGC AGCCTTATGT GGAATCAAAT
    CTTTTAGTTC CTATGGGTGG TGTAGGAGGA CAGGTTCAAG TGTCCCAGCC AGGAGGGAGT
    TTAGCACAAG CCCCCACTAC ATCCTCCCAG CAAGCAGTTT TGGAGAGTAC TCAGGGAGTC
    TCTCAGGTTG CTCCTGCAGA GCCAGTTGCA GTAGCACAGA CCCAAGCTAC CCAGCCGACC
    ACTTTGGCTT CCTCTGTAGA CAGTGCACAT TCAGATGTTG CTTCAGGTAT GAGTGATGGC
    AATGAGAACG TCCCATCTTC CAGTGGAAGG CATGAAGGAA GAACTACAAA ACGGCATTAC
    CGAAAATCTG TAAGGAGTCG CTCTCGACAT GAAAAAACTT CACGCCCAAA ATTAAGAATT
    TTGAATGTTT CAAATAAAGG AGACCGAGTA GTAGAATGTC AATTAGAGAC TCATAATAGG
    AAAATGGTTA CATTCAAATT TGACCTAGAT GGTGACAACC CCGAGGAGAT AGCAACAATT
    ATGGTGAACA ATGACTTTAT TCTAGCAATA GAGAGAGAGT CGTTTGTGGA TCAAGTGCGA
    GAAATTATTG AAAAAGCTGA TGAAATGCTC AGTGAGGATG TCAGTGTGGA ACCAGAGGGT
    GATCAGGGAT TGGAGAGTCT ACAAGGAAAG GATGACTATG GCTTTTCAGG TTCTCAGAAA
    TTGGAAGGAG AGTTCAAACA ACCAATTCCT GCGTCTTCCA TGCCACAGCA AATAGGCATT
    CCTACCAGTT CTTTAACTCA AGTTGTTCAT TCTGCGGGAA GGCGGTTTAT AGTGAGTCCT
    GTGCCAGAAA GCCGATTACG AGAATCAAAA GTTTTCCCCA GTGAAATAAC AGATACAGTT
    GCTGCCTCTA CAGCTCAGAG CCCTGGAATG AACTTGTCTC ACTCTGCATC ATCCCTTAGT
    CTACAACAGG CCTTTTCTGA ACTTAGACGT GCCCAAATGA CAGAAGGACC CAACACAGCA
    CCTCCAAACT TTAGTCATAC AGGACCAACA TTTCCAGTAG TACCTCCTTT CTTAAGTAGC
    ATTGCTGGAG TCCCAACCAC AGCAGCAGCC ACAGCACCAG TCCCTGCAAC AAGCAGCCCT
    CCTAATGACA TTTCCACATC AGTAATTCAG TCTGAGGTTA CAGTGCCCAC TGAAGAGGGG
    ATTGCTGGAG TTGCCACCAG CACAGGTGTG GTAACTTCAG GTGGTCTCCC CATACCACCT
    GTGTCTGAAT CACCAGTACT TTCCAGCGTA GTTTCAAGTA TCACAATACC TGCAGTTGTC
    TCAATATCTA CTACATCCCC GTCACTTCAA GTCCCCACAT CCACATCTGA GATCGTTGTT
    TCTAGTACAG CACTGTATCC TTCAGTAACA GTTTCAGCAA CTTCAGCCTC TGCAGGGGGC
    AGTACTGCTA CCCCAGGTCC TAAGCCTCCA GCTGTAGTAT CTCAGCAGGC AGCAGGCAGC
    ACTACTGTGG GAGCCACATT AACATCAGTT TCTACCACCA CTTCATTCCC AAGCACAGCT
    TCACAGCTGT GCATTCAGCT TAGCAGCAGT ACTTCTACTC CTACTTTAGC TGAAACCGTG
    GTAGTTAGCG CACACTCACT AGATAAGACA TCTCATAGCA GTACAACTGG ATTGGCTTTC
    TCCCTCTCTG CACCATCTTC CTCTTCCTCT CCTGGAGCAG GAGTGTCTAG TTATATTTCT
    CAGCCTGGTG GGCTGCATCC TTTGGTCATT CCATCAGTGA TAGCTTCTAC TCCTATTCTT
    CCCCAAGCAG CAGGACCTAC TTCTACACCT TTATTACCCC AAGTACCTAG TATCCCACCC
    TTGGTACAGC CTGTTGCCAA TGTGCCTGCT GTACAGCAGA CACTAATTCA TAGTCAGCCT
    CAACCAGCTT TGCTTCCCAA CCAGCCCCAT ACTCATTGTC CTGAAGTAGA TTCTGATACA
    CAACCCAAAG CTCCTGGAAT TGATGACATA AAGACTCTAG AAGAAAAGCT GCGGTCTCTG
    TTCAGTGAAC ACAGCTCATC TGGAGCTCAG CATGCCTCTG TCTCACTGGA GACCTCACTA
    GTCATAGAGA GCACTGTCAC ACCAGGCATC CCAACTACTG CTGTTGCACC AAGCAAACTC
    CTGACTTCTA CCACAAGTAC TTGCTTACCA CCAACCAATT TACCACTAGG AACAGTTGCT
    TTGCCAGTTA CACCAGTGGT CACACCTGGG CAAGTTTCTA CCCCAGTCAG CACTACTACA
    TCAGGAGTGA AACCTGGAAC TGCTCCCTCC AAGCCACCTC TAACTAAGGC TCCGGTGCTG
    CCAGTGGGTA CTGAACTTCC AGCAGGTACT CTACCCAGCG AGCAGCTGCC ACCTTTTCCA
    GGACCTTCTC TAACCCAGTC CCAGCAACCT CTAGAGGATC TTGATGCTCA ATTGAGAAGA
    ACACTTAGTC CAGAGATGAT CACAGTGACT TCTGCGGTTG GTCCTGTGTC CATGGCGGCT
    CCAACAGCAA TCACAGAAGC AGGAACACAG CCTCAGAAGG GTGTTTCTCA AGTCAAAGAA
    GGCCCTGTCC TAGCAACTAG TTCAGGAGCT GGTGTTTTTA AGATGGGACG ATTTCAGGTT
    TCTGTTGCAG CAGACGGTGC CCAGAAAGAG GGTAAAAATA AGTCAGAAGA TGCAAAGTCT
    GTTCATTTTG AATCCAGCAC CTCAGAGTCC TCAGTGCTAT CAAGTAGTAG TCCAGAGAGT
    ACCTTGGTGA AACCAGAGCC GAATGGCATA ACCATCCCTG GTATCTCTTC AGATGTGCCA
    GAGAGTGCCC ACAAAACTAC TGCCTCAGAG GCAAAGTCAG ACACTGGGCA GCCTACCAAG
    GTTGGACGTT TTCAGGTGAC AACTACAGCA AACAAAGTGG GTCGTTTCTC TGTATCAAAA
    ACTGAGGACA AGATCACTGA CACAAAGAAA GAAGGACCAG TGGCATCTCC TCCTTTTATG
    GATTTGGAAC AAGCTGTTCT TCCTGCTGTG ATACCAAAGA AAGAGAAGCC TGAACTGTCA
    GAGCCTTCAC ATCTAAATGG GCCGTCTTCT GACCCGGAGG CCGCTTTTTT AAGTAGGGAT
    GTGGATGATG GTTCCGGTAG TCCACACTCG CCCCATCAGC TGAGCTCAAA GAGCCTTCCT
    AGCCAGAATC TAAGTCAAAG CCTTAGTAAT TCATTTAACT CCTCTTACAT GAGTAGCGAC
    AATGAGTCAG ATATCGAAGA TGAAGACTTA AAGTTAGAGC TGCGACGACT ACGAGATAAA
    CATCTCAAAG AGATTCAGGA CCTGCAGAGT CGCCAGAAGC ATGAAATTGA ATCTTTGTAT
    ACCAAACTGG GCAAGGTGCC CCCTGCTGTT ATTATTCCCC CAGCTGCTCC CCTTTCAGGG
    AGAAGACGAC GACCCACTAA AAGCAAAGGC AGCAAATCTA GTCGAAGCAG TTCCTTGGGG
    AATAAAAGCC CCCAGCTTTC AGGTAACCTG TCTGGTCAGA GTGCAGCTTC AGTCTTGCAC
    CCCCAGCAGA CCCTCCACCC TCCTGGCAAC ATCCCAGAGT CCGGGCAGAA TCAGCTGTTA
    CAGCCCCTTA AGCCATCTCC CTCCAGTGAC AACCTCTATT CAGCCTTCAC CAGTGATGGT
    GCCATTTCAG TACCAAGCCT TTCTGCTCCA GGTCAAGGAA CCAGCAGCAC AAACACTGTT
    GGGGCAACAG TGAACAGCCA AGCCGCCCAA GCTCAGCCTC CTGCCATGAC GTCCAGCAGG
    AAGGGCACAT TCACAGATGA CTTGCACAAG TTGGTAGACA ATTGGGCCCG AGATGCCATG
    AATCTCTCAG GCAGGAGAGG AAGCAAAGGG CACATGAATT ACGAGGGCCC TGGAATGGCA
    AGGAAGTTCT CTGCACCTGG GCAACTGTGC ATCTCCATGA CCTCGAACCT GGGTGGCTCT
    GCCCCCATCT CTGCAGCATC AGCTACCTCT CTAGGTCACT TCACCAAGTC TATGTGCCCC
    CCACAGCAGT ATGGCTTTCC AGCTACCCCA TTTGGCGCTC AATGGAGTGG GACGGGTGGC
    CCAGCACCAC AGCCACTTGG CCAGTTCCAA CCTGTGGGAA CTGCCTCCTT GCAGAATTTC
    AACATCAGCA ATTTGCAGAA ATCCATCAGC AACCCCCCAG GCTCCAACCT GCGGACCACT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016875325.1
    CDS969..7571
    Translation

    Target ORF information:

    RefSeq Version XM_017019836.1
    Organism Homo sapiens(human)
    Definition Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017019836.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
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    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
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    1981
    2041
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    2161
    2221
    2281
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    2701
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    3001
    3061
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    3181
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    3301
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    3661
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    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGGGAATACA GCAGACAGCC
    CCTCCTCAAC AGACAGTGCA GTATTCACTT TCACAGACAT CAACCTCCAG TGAGGCCACT
    ACTGCACAGC CAGTGAGTCA GCCTCAAGCT CCACAAGTCT TGCCTCAAGT ATCAGCTGGA
    AAACAGGGCT TCCCACCTCG ACTGCCACCA CAGTACCCAG GAGATTCAAA TATTGCTCCC
    TCTTCCAACG TGGCTTCTGT TTGCATCCAT TCTACAGTCC TATCCCCTCC CATGCCGACA
    GAAGTACTGG CTACACCTGG GTACTTTCCC ACAGTGGTGC AGCCTTATGT GGAATCAAAT
    CTTTTAGTTC CTATGGGTGG TGTAGGAGGA CAGGTTCAAG TGTCCCAGCC AGGAGGGAGT
    TTAGCACAAG CCCCCACTAC ATCCTCCCAG CAAGCAGTTT TGGAGAGTAC TCAGGGAGTC
    TCTCAGGTTG CTCCTGCAGA GCCAGTTGCA GTAGCACAGA CCCAAGCTAC CCAGCCGACC
    ACTTTGGCTT CCTCTGTAGA CAGTGCACAT TCAGATGTTG CTTCAGGTAT GAGTGATGGC
    AATGAGAACG TCCCATCTTC CAGTGGAAGG CATGAAGGAA GAACTACAAA ACGGCATTAC
    CGAAAATCTG TAAGGAGTCG CTCTCGACAT GAAAAAACTT CACGCCCAAA ATTAAGAATT
    TTGAATGTTT CAAATAAAGG AGACCGAGTA GTAGAATGTC AATTAGAGAC TCATAATAGG
    AAAATGGTTA CATTCAAATT TGACCTAGAT GGTGACAACC CCGAGGAGAT AGCAACAATT
    ATGGTGAACA ATGACTTTAT TCTAGCAATA GAGAGAGAGT CGTTTGTGGA TCAAGTGCGA
    GAAATTATTG AAAAAGCTGA TGAAATGCTC AGTGAGGATG TCAGTGTGGA ACCAGAGGGT
    GATCAGGGAT TGGAGAGTCT ACAAGGAAAG GATGACTATG GCTTTTCAGG TTCTCAGAAA
    TTGGAAGGAG AGTTCAAACA ACCAATTCCT GCGTCTTCCA TGCCACAGCA AATAGGCATT
    CCTACCAGTT CTTTAACTCA AGTTGTTCAT TCTGCGGGAA GGCGGTTTAT AGTGAGTCCT
    GTGCCAGAAA GCCGATTACG AGAATCAAAA GTTTTCCCCA GTGAAATAAC AGATACAGTT
    GCTGCCTCTA CAGCTCAGAG CCCTGGAATG AACTTGTCTC ACTCTGCATC ATCCCTTAGT
    CTACAACAGG CCTTTTCTGA ACTTAGACGT GCCCAAATGA CAGAAGGACC CAACACAGCA
    CCTCCAAACT TTAGTCATAC AGGACCAACA TTTCCAGTAG TACCTCCTTT CTTAAGTAGC
    ATTGCTGGAG TCCCAACCAC AGCAGCAGCC ACAGCACCAG TCCCTGCAAC AAGCAGCCCT
    CCTAATGACA TTTCCACATC AGTAATTCAG TCTGAGGTTA CAGTGCCCAC TGAAGAGGGG
    ATTGCTGGAG TTGCCACCAG CACAGGTGTG GTAACTTCAG GTGGTCTCCC CATACCACCT
    GTGTCTGAAT CACCAGTACT TTCCAGCGTA GTTTCAAGTA TCACAATACC TGCAGTTGTC
    TCAATATCTA CTACATCCCC GTCACTTCAA GTCCCCACAT CCACATCTGA GATCGTTGTT
    TCTAGTACAG CACTGTATCC TTCAGTAACA GTTTCAGCAA CTTCAGCCTC TGCAGGGGGC
    AGTACTGCTA CCCCAGGTCC TAAGCCTCCA GCTGTAGTAT CTCAGCAGGC AGCAGGCAGC
    ACTACTGTGG GAGCCACATT AACATCAGTT TCTACCACCA CTTCATTCCC AAGCACAGCT
    TCACAGCTGT GCATTCAGCT TAGCAGCAGT ACTTCTACTC CTACTTTAGC TGAAACCGTG
    GTAGTTAGCG CACACTCACT AGATAAGACA TCTCATAGCA GTACAACTGG ATTGGCTTTC
    TCCCTCTCTG CACCATCTTC CTCTTCCTCT CCTGGAGCAG GAGTGTCTAG TTATATTTCT
    CAGCCTGGTG GGCTGCATCC TTTGGTCATT CCATCAGTGA TAGCTTCTAC TCCTATTCTT
    CCCCAAGCAG CAGGACCTAC TTCTACACCT TTATTACCCC AAGTACCTAG TATCCCACCC
    TTGGTACAGC CTGTTGCCAA TGTGCCTGCT GTACAGCAGA CACTAATTCA TAGTCAGCCT
    CAACCAGCTT TGCTTCCCAA CCAGCCCCAT ACTCATTGTC CTGAAGTAGA TTCTGATACA
    CAACCCAAAG CTCCTGGAAT TGATGACATA AAGACTCTAG AAGAAAAGCT GCGGTCTCTG
    TTCAGTGAAC ACAGCTCATC TGGAGCTCAG CATGCCTCTG TCTCACTGGA GACCTCACTA
    GTCATAGAGA GCACTGTCAC ACCAGGCATC CCAACTACTG CTGTTGCACC AAGCAAACTC
    CTGACTTCTA CCACAAGTAC TTGCTTACCA CCAACCAATT TACCACTAGG AACAGTTGCT
    TTGCCAGTTA CACCAGTGGT CACACCTGGG CAAGTTTCTA CCCCAGTCAG CACTACTACA
    TCAGGAGTGA AACCTGGAAC TGCTCCCTCC AAGCCACCTC TAACTAAGGC TCCGGTGCTG
    CCAGTGGGTA CTGAACTTCC AGCAGGTACT CTACCCAGCG AGCAGCTGCC ACCTTTTCCA
    GGACCTTCTC TAACCCAGTC CCAGCAACCT CTAGAGGATC TTGATGCTCA ATTGAGAAGA
    ACACTTAGTC CAGAGATGAT CACAGTGACT TCTGCGGTTG GTCCTGTGTC CATGGCGGCT
    CCAACAGCAA TCACAGAAGC AGGAACACAG CCTCAGAAGG GTGTTTCTCA AGTCAAAGAA
    GGCCCTGTCC TAGCAACTAG TTCAGGAGCT GGTGTTTTTA AGATGGGACG ATTTCAGGTT
    TCTGTTGCAG CAGACGGTGC CCAGAAAGAG GGTAAAAATA AGTCAGAAGA TGCAAAGTCT
    GTTCATTTTG AATCCAGCAC CTCAGAGTCC TCAGTGCTAT CAAGTAGTAG TCCAGAGAGT
    ACCTTGGTGA AACCAGAGCC GAATGGCATA ACCATCCCTG GTATCTCTTC AGATGTGCCA
    GAGAGTGCCC ACAAAACTAC TGCCTCAGAG GCAAAGTCAG ACACTGGGCA GCCTACCAAG
    GTTGGACGTT TTCAGGTGAC AACTACAGCA AACAAAGTGG GTCGTTTCTC TGTATCAAAA
    ACTGAGGACA AGATCACTGA CACAAAGAAA GAAGGACCAG TGGCATCTCC TCCTTTTATG
    GATTTGGAAC AAGCTGTTCT TCCTGCTGTG ATACCAAAGA AAGAGAAGCC TGAACTGTCA
    GAGCCTTCAC ATCTAAATGG GCCGTCTTCT GACCCGGAGG CCGCTTTTTT AAGTAGGGAT
    GTGGATGATG GTTCCGGTAG TCCACACTCG CCCCATCAGC TGAGCTCAAA GAGCCTTCCT
    AGCCAGAATC TAAGTCAAAG CCTTAGTAAT TCATTTAACT CCTCTTACAT GAGTAGCGAC
    AATGAGTCAG ATATCGAAGA TGAAGACTTA AAGTTAGAGC TGCGACGACT ACGAGATAAA
    CATCTCAAAG AGATTCAGGA CCTGCAGAGT CGCCAGAAGC ATGAAATTGA ATCTTTGTAT
    ACCAAACTGG GCAAGGTGCC CCCTGCTGTT ATTATTCCCC CAGCTGCTCC CCTTTCAGGG
    AGAAGACGAC GACCCACTAA AAGCAAAGGC AGCAAATCTA GTCGAAGCAG TTCCTTGGGG
    AATAAAAGCC CCCAGCTTTC AGGTAACCTG TCTGGTCAGA GTGCAGCTTC AGTCTTGCAC
    CCCCAGCAGA CCCTCCACCC TCCTGGCAAC ATCCCAGAGT CCGGGCAGAA TCAGCTGTTA
    CAGCCCCTTA AGCCATCTCC CTCCAGTGAC AACCTCTATT CAGCCTTCAC CAGTGATGGT
    GCCATTTCAG TACCAAGCCT TTCTGCTCCA GGTCAAGGAA CCAGCAGCAC AAACACTGTT
    GGGGCAACAG TGAACAGCCA AGCCGCCCAA GCTCAGCCTC CTGCCATGAC GTCCAGCAGG
    AAGGGCACAT TCACAGATGA CTTGCACAAG TTGGTAGACA ATTGGGCCCG AGATGCCATG
    AATCTCTCAG GCAGGAGAGG AAGCAAAGGG CACATGAATT ACGAGGGCCC TGGAATGGCA
    AGGAAGTTCT CTGCACCTGG GCAACTGTGC ATCTCCATGA CCTCGAACCT GGGTGGCTCT
    GCCCCCATCT CTGCAGCATC AGCTACCTCT CTAGGTCACT TCACCAAGTC TATGTGCCCC
    CCACAGCAGT ATGGCTTTCC AGCTACCCCA TTTGGCGCTC AATGGAGTGG GACGGGTGGC
    CCAGCACCAC AGCCACTTGG CCAGTTCCAA CCTGTGGGAA CTGCCTCCTT GCAGAATTTC
    AACATCAGCA ATTTGCAGAA ATCCATCAGC AACCCCCCAG GCTCCAACCT GCGGACCACT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu84077
    Clone ID Related Accession (Same CDS sequence) XM_017019837.1
    Accession Version XM_017019837.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6411bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase WNK1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT
    CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AACCCATACA ACATCCTCAG
    CAGCAGCAGG GAATACAGCA GACAGCCCCT CCTCAACAGA CAGTGCAGTA TTCACTTTCA
    CAGACATCAA CCTCCAGTGA GGCCACTACT GCACAGCCAG TGAGTCAGCC TCAAGCTCCA
    CAAGTCTTGC CTCAAGTATC AGCTGGAAAA CAGAGTACTC AGGGAGTCTC TCAGGTTGCT
    CCTGCAGAGC CAGTTGCAGT AGCACAGACC CAAGCTACCC AGCCGACCAC TTTGGCTTCC
    TCTGTAGACA GTGCACATTC AGATGTTGCT TCAGGTATGA GTGATGGCAA TGAGAACGTC
    CCATCTTCCA GTGGAAGGCA TGAAGGAAGA ACTACAAAAC GGCATTACCG AAAATCTGTA
    AGGAGTCGCT CTCGACATGA AAAAACTTCA CGCCCAAAAT TAAGAATTTT GAATGTTTCA
    AATAAAGGAG ACCGAGTAGT AGAATGTCAA TTAGAGACTC ATAATAGGAA AATGGTTACA
    TTCAAATTTG ACCTAGATGG TGACAACCCC GAGGAGATAG CAACAATTAT GGTGAACAAT
    GACTTTATTC TAGCAATAGA GAGAGAGTCG TTTGTGGATC AAGTGCGAGA AATTATTGAA
    AAAGCTGATG AAATGCTCAG TGAGGATGTC AGTGTGGAAC CAGAGGGTGA TCAGGGATTG
    GAGAGTCTAC AAGGAAAGGA TGACTATGGC TTTTCAGGTT CTCAGAAATT GGAAGGAGAG
    TTCAAACAAC CAATTCCTGC GTCTTCCATG CCACAGCAAA TAGGCATTCC TACCAGTTCT
    TTAACTCAAG TTGTTCATTC TGCGGGAAGG CGGTTTATAG TGAGTCCTGT GCCAGAAAGC
    CGATTACGAG AATCAAAAGT TTTCCCCAGT GAAATAACAG ATACAGTTGC TGCCTCTACA
    GCTCAGAGCC CTGGAATGAA CTTGTCTCAC TCTGCATCAT CCCTTAGTCT ACAACAGGCC
    TTTTCTGAAC TTAGACGTGC CCAAATGACA GAAGGACCCA ACACAGCACC TCCAAACTTT
    AGTCATACAG GACCAACATT TCCAGTAGTA CCTCCTTTCT TAAGTAGCAT TGCTGGAGTC
    CCAACCACAG CAGCAGCCAC AGCACCAGTC CCTGCAACAA GCAGCCCTCC TAATGACATT
    TCCACATCAG TAATTCAGTC TGAGGTTACA GTGCCCACTG AAGAGGGGAT TGCTGGAGTT
    GCCACCAGCA CAGGTGTGGT AACTTCAGGT GGTCTCCCCA TACCACCTGT GTCTGAATCA
    CCAGTACTTT CCAGCGTAGT TTCAAGTATC ACAATACCTG CAGTTGTCTC AATATCTACT
    ACATCCCCGT CACTTCAAGT CCCCACATCC ACATCTGAGA TCGTTGTTTC TAGTACAGCA
    CTGTATCCTT CAGTAACAGT TTCAGCAACT TCAGCCTCTG CAGGGGGCAG TACTGCTACC
    CCAGGTCCTA AGCCTCCAGC TGTAGTATCT CAGCAGGCAG CAGGCAGCAC TACTGTGGGA
    GCCACATTAA CATCAGTTTC TACCACCACT TCATTCCCAA GCACAGCTTC ACAGCTGTGC
    ATTCAGCTTA GCAGCAGTAC TTCTACTCCT ACTTTAGCTG AAACCGTGGT AGTTAGCGCA
    CACTCACTAG ATAAGACATC TCATAGCAGT ACAACTGGAT TGGCTTTCTC CCTCTCTGCA
    CCATCTTCCT CTTCCTCTCC TGGAGCAGGA GTGTCTAGTT ATATTTCTCA GCCTGGTGGG
    CTGCATCCTT TGGTCATTCC ATCAGTGATA GCTTCTACTC CTATTCTTCC CCAAGCAGCA
    GGACCTACTT CTACACCTTT ATTACCCCAA GTACCTAGTA TCCCACCCTT GGTACAGCCT
    GTTGCCAATG TGCCTGCTGT ACAGCAGACA CTAATTCATA GTCAGCCTCA ACCAGCTTTG
    CTTCCCAACC AGCCCCATAC TCATTGTCCT GAAGTAGATT CTGATACACA ACCCAAAGCT
    CCTGGAATTG ATGACATAAA GACTCTAGAA GAAAAGCTGC GGTCTCTGTT CAGTGAACAC
    AGCTCATCTG GAGCTCAGCA TGCCTCTGTC TCACTGGAGA CCTCACTAGT CATAGAGAGC
    ACTGTCACAC CAGGCATCCC AACTACTGCT GTTGCACCAA GCAAACTCCT GACTTCTACC
    ACAAGTACTT GCTTACCACC AACCAATTTA CCACTAGGAA CAGTTGCTTT GCCAGTTACA
    CCAGTGGTCA CACCTGGGCA AGTTTCTACC CCAGTCAGCA CTACTACATC AGGAGTGAAA
    CCTGGAACTG CTCCCTCCAA GCCACCTCTA ACTAAGGCTC CGGTGCTGCC AGTGGGTACT
    GAACTTCCAG CAGGTACTCT ACCCAGCGAG CAGCTGCCAC CTTTTCCAGG ACCTTCTCTA
    ACCCAGTCCC AGCAACCTCT AGAGGATCTT GATGCTCAAT TGAGAAGAAC ACTTAGTCCA
    GAGATGATCA CAGTGACTTC TGCGGTTGGT CCTGTGTCCA TGGCGGCTCC AACAGCAATC
    ACAGAAGCAG GAACACAGCC TCAGAAGGGT GTTTCTCAAG TCAAAGAAGG CCCTGTCCTA
    GCAACTAGTT CAGGAGCTGG TGTTTTTAAG ATGGGACGAT TTCAGGTTTC TGTTGCAGCA
    GACGGTGCCC AGAAAGAGGG TAAAAATAAG TCAGAAGATG CAAAGTCTGT TCATTTTGAA
    TCCAGCACCT CAGAGTCCTC AGTGCTATCA AGTAGTAGTC CAGAGAGTAC CTTGGTGAAA
    CCAGAGCCGA ATGGCATAAC CATCCCTGGT ATCTCTTCAG ATGTGCCAGA GAGTGCCCAC
    AAAACTACTG CCTCAGAGGC AAAGTCAGAC ACTGGGCAGC CTACCAAGGT TGGACGTTTT
    CAGGTGACAA CTACAGCAAA CAAAGTGGGT CGTTTCTCTG TATCAAAAAC TGAGGACAAG
    ATCACTGACA CAAAGAAAGA AGGACCAGTG GCATCTCCTC CTTTTATGGA TTTGGAACAA
    GCTGTTCTTC CTGCTGTGAT ACCAAAGAAA GAGAAGCCTG AACTGTCAGA GCCTTCACAT
    CTAAATGGGC CGTCTTCTGA CCCGGAGGCC GCTTTTTTAA GTAGGGATGT GGATGATGGT
    TCCGGTAGTC CACACTCGCC CCATCAGCTG AGCTCAAAGA GCCTTCCTAG CCAGAATCTA
    AGTCAAAGCC TTAGTAATTC ATTTAACTCC TCTTACATGA GTAGCGACAA TGAGTCAGAT
    ATCGAAGATG AAGACTTAAA GTTAGAGCTG CGACGACTAC GAGATAAACA TCTCAAAGAG
    ATTCAGGACC TGCAGAGTCG CCAGAAGCAT GAAATTGAAT CTTTGTATAC CAAACTGGGC
    AAGGTGCCCC CTGCTGTTAT TATTCCCCCA GCTGCTCCCC TTTCAGGGAG AAGACGACGA
    CCCACTAAAA GCAAAGGCAG CAAATCTAGT CGAAGCAGTT CCTTGGGGAA TAAAAGCCCC
    CAGCTTTCAG GTAACCTGTC TGGTCAGAGT GCAGCTTCAG TCTTGCACCC CCAGCAGACC
    CTCCACCCTC CTGGCAACAT CCCAGAGTCC GGGCAGAATC AGCTGTTACA GCCCCTTAAG
    CCATCTCCCT CCAGTGACAA CCTCTATTCA GCCTTCACCA GTGATGGTGC CATTTCAGTA
    CCAAGCCTTT CTGCTCCAGG TCAAGGAACC AGCAGCACAA ACACTGTTGG GGCAACAGTG
    AACAGCCAAG CCGCCCAAGC TCAGCCTCCT GCCATGACGT CCAGCAGGAA GGGCACATTC
    ACAGATGACT TGCACAAGTT GGTAGACAAT TGGGCCCGAG ATGCCATGAA TCTCTCAGGC
    AGGAGAGGAA GCAAAGGGCA CATGAATTAC GAGGGCCCTG GAATGGCAAG GAAGTTCTCT
    GCACCTGGGC AACTGTGCAT CTCCATGACC TCGAACCTGG GTGGCTCTGC CCCCATCTCT
    GCAGCATCAG CTACCTCTCT AGGTCACTTC ACCAAGTCTA TGTGCCCCCC ACAGCAGTAT
    GGCTTTCCAG CTACCCCATT TGGCGCTCAA TGGAGTGGGA CGGGTGGCCC AGCACCACAG
    CCACTTGGCC AGTTCCAACC TGTGGGAACT GCCTCCTTGC AGAATTTCAA CATCAGCAAT
    TTGCAGAAAT CCATCAGCAA CCCCCCAGGC TCCAACCTGC GGACCACTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016875326.1
    CDS969..7379
    Translation

    Target ORF information:

    RefSeq Version XM_017019837.1
    Organism Homo sapiens(human)
    Definition Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017019837.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT
    CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AACCCATACA ACATCCTCAG
    CAGCAGCAGG GAATACAGCA GACAGCCCCT CCTCAACAGA CAGTGCAGTA TTCACTTTCA
    CAGACATCAA CCTCCAGTGA GGCCACTACT GCACAGCCAG TGAGTCAGCC TCAAGCTCCA
    CAAGTCTTGC CTCAAGTATC AGCTGGAAAA CAGAGTACTC AGGGAGTCTC TCAGGTTGCT
    CCTGCAGAGC CAGTTGCAGT AGCACAGACC CAAGCTACCC AGCCGACCAC TTTGGCTTCC
    TCTGTAGACA GTGCACATTC AGATGTTGCT TCAGGTATGA GTGATGGCAA TGAGAACGTC
    CCATCTTCCA GTGGAAGGCA TGAAGGAAGA ACTACAAAAC GGCATTACCG AAAATCTGTA
    AGGAGTCGCT CTCGACATGA AAAAACTTCA CGCCCAAAAT TAAGAATTTT GAATGTTTCA
    AATAAAGGAG ACCGAGTAGT AGAATGTCAA TTAGAGACTC ATAATAGGAA AATGGTTACA
    TTCAAATTTG ACCTAGATGG TGACAACCCC GAGGAGATAG CAACAATTAT GGTGAACAAT
    GACTTTATTC TAGCAATAGA GAGAGAGTCG TTTGTGGATC AAGTGCGAGA AATTATTGAA
    AAAGCTGATG AAATGCTCAG TGAGGATGTC AGTGTGGAAC CAGAGGGTGA TCAGGGATTG
    GAGAGTCTAC AAGGAAAGGA TGACTATGGC TTTTCAGGTT CTCAGAAATT GGAAGGAGAG
    TTCAAACAAC CAATTCCTGC GTCTTCCATG CCACAGCAAA TAGGCATTCC TACCAGTTCT
    TTAACTCAAG TTGTTCATTC TGCGGGAAGG CGGTTTATAG TGAGTCCTGT GCCAGAAAGC
    CGATTACGAG AATCAAAAGT TTTCCCCAGT GAAATAACAG ATACAGTTGC TGCCTCTACA
    GCTCAGAGCC CTGGAATGAA CTTGTCTCAC TCTGCATCAT CCCTTAGTCT ACAACAGGCC
    TTTTCTGAAC TTAGACGTGC CCAAATGACA GAAGGACCCA ACACAGCACC TCCAAACTTT
    AGTCATACAG GACCAACATT TCCAGTAGTA CCTCCTTTCT TAAGTAGCAT TGCTGGAGTC
    CCAACCACAG CAGCAGCCAC AGCACCAGTC CCTGCAACAA GCAGCCCTCC TAATGACATT
    TCCACATCAG TAATTCAGTC TGAGGTTACA GTGCCCACTG AAGAGGGGAT TGCTGGAGTT
    GCCACCAGCA CAGGTGTGGT AACTTCAGGT GGTCTCCCCA TACCACCTGT GTCTGAATCA
    CCAGTACTTT CCAGCGTAGT TTCAAGTATC ACAATACCTG CAGTTGTCTC AATATCTACT
    ACATCCCCGT CACTTCAAGT CCCCACATCC ACATCTGAGA TCGTTGTTTC TAGTACAGCA
    CTGTATCCTT CAGTAACAGT TTCAGCAACT TCAGCCTCTG CAGGGGGCAG TACTGCTACC
    CCAGGTCCTA AGCCTCCAGC TGTAGTATCT CAGCAGGCAG CAGGCAGCAC TACTGTGGGA
    GCCACATTAA CATCAGTTTC TACCACCACT TCATTCCCAA GCACAGCTTC ACAGCTGTGC
    ATTCAGCTTA GCAGCAGTAC TTCTACTCCT ACTTTAGCTG AAACCGTGGT AGTTAGCGCA
    CACTCACTAG ATAAGACATC TCATAGCAGT ACAACTGGAT TGGCTTTCTC CCTCTCTGCA
    CCATCTTCCT CTTCCTCTCC TGGAGCAGGA GTGTCTAGTT ATATTTCTCA GCCTGGTGGG
    CTGCATCCTT TGGTCATTCC ATCAGTGATA GCTTCTACTC CTATTCTTCC CCAAGCAGCA
    GGACCTACTT CTACACCTTT ATTACCCCAA GTACCTAGTA TCCCACCCTT GGTACAGCCT
    GTTGCCAATG TGCCTGCTGT ACAGCAGACA CTAATTCATA GTCAGCCTCA ACCAGCTTTG
    CTTCCCAACC AGCCCCATAC TCATTGTCCT GAAGTAGATT CTGATACACA ACCCAAAGCT
    CCTGGAATTG ATGACATAAA GACTCTAGAA GAAAAGCTGC GGTCTCTGTT CAGTGAACAC
    AGCTCATCTG GAGCTCAGCA TGCCTCTGTC TCACTGGAGA CCTCACTAGT CATAGAGAGC
    ACTGTCACAC CAGGCATCCC AACTACTGCT GTTGCACCAA GCAAACTCCT GACTTCTACC
    ACAAGTACTT GCTTACCACC AACCAATTTA CCACTAGGAA CAGTTGCTTT GCCAGTTACA
    CCAGTGGTCA CACCTGGGCA AGTTTCTACC CCAGTCAGCA CTACTACATC AGGAGTGAAA
    CCTGGAACTG CTCCCTCCAA GCCACCTCTA ACTAAGGCTC CGGTGCTGCC AGTGGGTACT
    GAACTTCCAG CAGGTACTCT ACCCAGCGAG CAGCTGCCAC CTTTTCCAGG ACCTTCTCTA
    ACCCAGTCCC AGCAACCTCT AGAGGATCTT GATGCTCAAT TGAGAAGAAC ACTTAGTCCA
    GAGATGATCA CAGTGACTTC TGCGGTTGGT CCTGTGTCCA TGGCGGCTCC AACAGCAATC
    ACAGAAGCAG GAACACAGCC TCAGAAGGGT GTTTCTCAAG TCAAAGAAGG CCCTGTCCTA
    GCAACTAGTT CAGGAGCTGG TGTTTTTAAG ATGGGACGAT TTCAGGTTTC TGTTGCAGCA
    GACGGTGCCC AGAAAGAGGG TAAAAATAAG TCAGAAGATG CAAAGTCTGT TCATTTTGAA
    TCCAGCACCT CAGAGTCCTC AGTGCTATCA AGTAGTAGTC CAGAGAGTAC CTTGGTGAAA
    CCAGAGCCGA ATGGCATAAC CATCCCTGGT ATCTCTTCAG ATGTGCCAGA GAGTGCCCAC
    AAAACTACTG CCTCAGAGGC AAAGTCAGAC ACTGGGCAGC CTACCAAGGT TGGACGTTTT
    CAGGTGACAA CTACAGCAAA CAAAGTGGGT CGTTTCTCTG TATCAAAAAC TGAGGACAAG
    ATCACTGACA CAAAGAAAGA AGGACCAGTG GCATCTCCTC CTTTTATGGA TTTGGAACAA
    GCTGTTCTTC CTGCTGTGAT ACCAAAGAAA GAGAAGCCTG AACTGTCAGA GCCTTCACAT
    CTAAATGGGC CGTCTTCTGA CCCGGAGGCC GCTTTTTTAA GTAGGGATGT GGATGATGGT
    TCCGGTAGTC CACACTCGCC CCATCAGCTG AGCTCAAAGA GCCTTCCTAG CCAGAATCTA
    AGTCAAAGCC TTAGTAATTC ATTTAACTCC TCTTACATGA GTAGCGACAA TGAGTCAGAT
    ATCGAAGATG AAGACTTAAA GTTAGAGCTG CGACGACTAC GAGATAAACA TCTCAAAGAG
    ATTCAGGACC TGCAGAGTCG CCAGAAGCAT GAAATTGAAT CTTTGTATAC CAAACTGGGC
    AAGGTGCCCC CTGCTGTTAT TATTCCCCCA GCTGCTCCCC TTTCAGGGAG AAGACGACGA
    CCCACTAAAA GCAAAGGCAG CAAATCTAGT CGAAGCAGTT CCTTGGGGAA TAAAAGCCCC
    CAGCTTTCAG GTAACCTGTC TGGTCAGAGT GCAGCTTCAG TCTTGCACCC CCAGCAGACC
    CTCCACCCTC CTGGCAACAT CCCAGAGTCC GGGCAGAATC AGCTGTTACA GCCCCTTAAG
    CCATCTCCCT CCAGTGACAA CCTCTATTCA GCCTTCACCA GTGATGGTGC CATTTCAGTA
    CCAAGCCTTT CTGCTCCAGG TCAAGGAACC AGCAGCACAA ACACTGTTGG GGCAACAGTG
    AACAGCCAAG CCGCCCAAGC TCAGCCTCCT GCCATGACGT CCAGCAGGAA GGGCACATTC
    ACAGATGACT TGCACAAGTT GGTAGACAAT TGGGCCCGAG ATGCCATGAA TCTCTCAGGC
    AGGAGAGGAA GCAAAGGGCA CATGAATTAC GAGGGCCCTG GAATGGCAAG GAAGTTCTCT
    GCACCTGGGC AACTGTGCAT CTCCATGACC TCGAACCTGG GTGGCTCTGC CCCCATCTCT
    GCAGCATCAG CTACCTCTCT AGGTCACTTC ACCAAGTCTA TGTGCCCCCC ACAGCAGTAT
    GGCTTTCCAG CTACCCCATT TGGCGCTCAA TGGAGTGGGA CGGGTGGCCC AGCACCACAG
    CCACTTGGCC AGTTCCAACC TGTGGGAACT GCCTCCTTGC AGAATTTCAA CATCAGCAAT
    TTGCAGAAAT CCATCAGCAA CCCCCCAGGC TCCAACCTGC GGACCACTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu84078
    Clone ID Related Accession (Same CDS sequence) XM_017019838.1
    Accession Version XM_017019838.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6408bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase WNK1 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT
    CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AACCCATACA ACATCCTCAG
    CAGCAGGGAA TACAGCAGAC AGCCCCTCCT CAACAGACAG TGCAGTATTC ACTTTCACAG
    ACATCAACCT CCAGTGAGGC CACTACTGCA CAGCCAGTGA GTCAGCCTCA AGCTCCACAA
    GTCTTGCCTC AAGTATCAGC TGGAAAACAG AGTACTCAGG GAGTCTCTCA GGTTGCTCCT
    GCAGAGCCAG TTGCAGTAGC ACAGACCCAA GCTACCCAGC CGACCACTTT GGCTTCCTCT
    GTAGACAGTG CACATTCAGA TGTTGCTTCA GGTATGAGTG ATGGCAATGA GAACGTCCCA
    TCTTCCAGTG GAAGGCATGA AGGAAGAACT ACAAAACGGC ATTACCGAAA ATCTGTAAGG
    AGTCGCTCTC GACATGAAAA AACTTCACGC CCAAAATTAA GAATTTTGAA TGTTTCAAAT
    AAAGGAGACC GAGTAGTAGA ATGTCAATTA GAGACTCATA ATAGGAAAAT GGTTACATTC
    AAATTTGACC TAGATGGTGA CAACCCCGAG GAGATAGCAA CAATTATGGT GAACAATGAC
    TTTATTCTAG CAATAGAGAG AGAGTCGTTT GTGGATCAAG TGCGAGAAAT TATTGAAAAA
    GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGATCA GGGATTGGAG
    AGTCTACAAG GAAAGGATGA CTATGGCTTT TCAGGTTCTC AGAAATTGGA AGGAGAGTTC
    AAACAACCAA TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA
    ACTCAAGTTG TTCATTCTGC GGGAAGGCGG TTTATAGTGA GTCCTGTGCC AGAAAGCCGA
    TTACGAGAAT CAAAAGTTTT CCCCAGTGAA ATAACAGATA CAGTTGCTGC CTCTACAGCT
    CAGAGCCCTG GAATGAACTT GTCTCACTCT GCATCATCCC TTAGTCTACA ACAGGCCTTT
    TCTGAACTTA GACGTGCCCA AATGACAGAA GGACCCAACA CAGCACCTCC AAACTTTAGT
    CATACAGGAC CAACATTTCC AGTAGTACCT CCTTTCTTAA GTAGCATTGC TGGAGTCCCA
    ACCACAGCAG CAGCCACAGC ACCAGTCCCT GCAACAAGCA GCCCTCCTAA TGACATTTCC
    ACATCAGTAA TTCAGTCTGA GGTTACAGTG CCCACTGAAG AGGGGATTGC TGGAGTTGCC
    ACCAGCACAG GTGTGGTAAC TTCAGGTGGT CTCCCCATAC CACCTGTGTC TGAATCACCA
    GTACTTTCCA GCGTAGTTTC AAGTATCACA ATACCTGCAG TTGTCTCAAT ATCTACTACA
    TCCCCGTCAC TTCAAGTCCC CACATCCACA TCTGAGATCG TTGTTTCTAG TACAGCACTG
    TATCCTTCAG TAACAGTTTC AGCAACTTCA GCCTCTGCAG GGGGCAGTAC TGCTACCCCA
    GGTCCTAAGC CTCCAGCTGT AGTATCTCAG CAGGCAGCAG GCAGCACTAC TGTGGGAGCC
    ACATTAACAT CAGTTTCTAC CACCACTTCA TTCCCAAGCA CAGCTTCACA GCTGTGCATT
    CAGCTTAGCA GCAGTACTTC TACTCCTACT TTAGCTGAAA CCGTGGTAGT TAGCGCACAC
    TCACTAGATA AGACATCTCA TAGCAGTACA ACTGGATTGG CTTTCTCCCT CTCTGCACCA
    TCTTCCTCTT CCTCTCCTGG AGCAGGAGTG TCTAGTTATA TTTCTCAGCC TGGTGGGCTG
    CATCCTTTGG TCATTCCATC AGTGATAGCT TCTACTCCTA TTCTTCCCCA AGCAGCAGGA
    CCTACTTCTA CACCTTTATT ACCCCAAGTA CCTAGTATCC CACCCTTGGT ACAGCCTGTT
    GCCAATGTGC CTGCTGTACA GCAGACACTA ATTCATAGTC AGCCTCAACC AGCTTTGCTT
    CCCAACCAGC CCCATACTCA TTGTCCTGAA GTAGATTCTG ATACACAACC CAAAGCTCCT
    GGAATTGATG ACATAAAGAC TCTAGAAGAA AAGCTGCGGT CTCTGTTCAG TGAACACAGC
    TCATCTGGAG CTCAGCATGC CTCTGTCTCA CTGGAGACCT CACTAGTCAT AGAGAGCACT
    GTCACACCAG GCATCCCAAC TACTGCTGTT GCACCAAGCA AACTCCTGAC TTCTACCACA
    AGTACTTGCT TACCACCAAC CAATTTACCA CTAGGAACAG TTGCTTTGCC AGTTACACCA
    GTGGTCACAC CTGGGCAAGT TTCTACCCCA GTCAGCACTA CTACATCAGG AGTGAAACCT
    GGAACTGCTC CCTCCAAGCC ACCTCTAACT AAGGCTCCGG TGCTGCCAGT GGGTACTGAA
    CTTCCAGCAG GTACTCTACC CAGCGAGCAG CTGCCACCTT TTCCAGGACC TTCTCTAACC
    CAGTCCCAGC AACCTCTAGA GGATCTTGAT GCTCAATTGA GAAGAACACT TAGTCCAGAG
    ATGATCACAG TGACTTCTGC GGTTGGTCCT GTGTCCATGG CGGCTCCAAC AGCAATCACA
    GAAGCAGGAA CACAGCCTCA GAAGGGTGTT TCTCAAGTCA AAGAAGGCCC TGTCCTAGCA
    ACTAGTTCAG GAGCTGGTGT TTTTAAGATG GGACGATTTC AGGTTTCTGT TGCAGCAGAC
    GGTGCCCAGA AAGAGGGTAA AAATAAGTCA GAAGATGCAA AGTCTGTTCA TTTTGAATCC
    AGCACCTCAG AGTCCTCAGT GCTATCAAGT AGTAGTCCAG AGAGTACCTT GGTGAAACCA
    GAGCCGAATG GCATAACCAT CCCTGGTATC TCTTCAGATG TGCCAGAGAG TGCCCACAAA
    ACTACTGCCT CAGAGGCAAA GTCAGACACT GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG
    GTGACAACTA CAGCAAACAA AGTGGGTCGT TTCTCTGTAT CAAAAACTGA GGACAAGATC
    ACTGACACAA AGAAAGAAGG ACCAGTGGCA TCTCCTCCTT TTATGGATTT GGAACAAGCT
    GTTCTTCCTG CTGTGATACC AAAGAAAGAG AAGCCTGAAC TGTCAGAGCC TTCACATCTA
    AATGGGCCGT CTTCTGACCC GGAGGCCGCT TTTTTAAGTA GGGATGTGGA TGATGGTTCC
    GGTAGTCCAC ACTCGCCCCA TCAGCTGAGC TCAAAGAGCC TTCCTAGCCA GAATCTAAGT
    CAAAGCCTTA GTAATTCATT TAACTCCTCT TACATGAGTA GCGACAATGA GTCAGATATC
    GAAGATGAAG ACTTAAAGTT AGAGCTGCGA CGACTACGAG ATAAACATCT CAAAGAGATT
    CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACCAA ACTGGGCAAG
    GTGCCCCCTG CTGTTATTAT TCCCCCAGCT GCTCCCCTTT CAGGGAGAAG ACGACGACCC
    ACTAAAAGCA AAGGCAGCAA ATCTAGTCGA AGCAGTTCCT TGGGGAATAA AAGCCCCCAG
    CTTTCAGGTA ACCTGTCTGG TCAGAGTGCA GCTTCAGTCT TGCACCCCCA GCAGACCCTC
    CACCCTCCTG GCAACATCCC AGAGTCCGGG CAGAATCAGC TGTTACAGCC CCTTAAGCCA
    TCTCCCTCCA GTGACAACCT CTATTCAGCC TTCACCAGTG ATGGTGCCAT TTCAGTACCA
    AGCCTTTCTG CTCCAGGTCA AGGAACCAGC AGCACAAACA CTGTTGGGGC AACAGTGAAC
    AGCCAAGCCG CCCAAGCTCA GCCTCCTGCC ATGACGTCCA GCAGGAAGGG CACATTCACA
    GATGACTTGC ACAAGTTGGT AGACAATTGG GCCCGAGATG CCATGAATCT CTCAGGCAGG
    AGAGGAAGCA AAGGGCACAT GAATTACGAG GGCCCTGGAA TGGCAAGGAA GTTCTCTGCA
    CCTGGGCAAC TGTGCATCTC CATGACCTCG AACCTGGGTG GCTCTGCCCC CATCTCTGCA
    GCATCAGCTA CCTCTCTAGG TCACTTCACC AAGTCTATGT GCCCCCCACA GCAGTATGGC
    TTTCCAGCTA CCCCATTTGG CGCTCAATGG AGTGGGACGG GTGGCCCAGC ACCACAGCCA
    CTTGGCCAGT TCCAACCTGT GGGAACTGCC TCCTTGCAGA ATTTCAACAT CAGCAATTTG
    CAGAAATCCA TCAGCAACCC CCCAGGCTCC AACCTGCGGA CCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016875327.1
    CDS969..7376
    Translation

    Target ORF information:

    RefSeq Version XM_017019838.1
    Organism Homo sapiens(human)
    Definition Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017019838.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT
    CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AACCCATACA ACATCCTCAG
    CAGCAGGGAA TACAGCAGAC AGCCCCTCCT CAACAGACAG TGCAGTATTC ACTTTCACAG
    ACATCAACCT CCAGTGAGGC CACTACTGCA CAGCCAGTGA GTCAGCCTCA AGCTCCACAA
    GTCTTGCCTC AAGTATCAGC TGGAAAACAG AGTACTCAGG GAGTCTCTCA GGTTGCTCCT
    GCAGAGCCAG TTGCAGTAGC ACAGACCCAA GCTACCCAGC CGACCACTTT GGCTTCCTCT
    GTAGACAGTG CACATTCAGA TGTTGCTTCA GGTATGAGTG ATGGCAATGA GAACGTCCCA
    TCTTCCAGTG GAAGGCATGA AGGAAGAACT ACAAAACGGC ATTACCGAAA ATCTGTAAGG
    AGTCGCTCTC GACATGAAAA AACTTCACGC CCAAAATTAA GAATTTTGAA TGTTTCAAAT
    AAAGGAGACC GAGTAGTAGA ATGTCAATTA GAGACTCATA ATAGGAAAAT GGTTACATTC
    AAATTTGACC TAGATGGTGA CAACCCCGAG GAGATAGCAA CAATTATGGT GAACAATGAC
    TTTATTCTAG CAATAGAGAG AGAGTCGTTT GTGGATCAAG TGCGAGAAAT TATTGAAAAA
    GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGATCA GGGATTGGAG
    AGTCTACAAG GAAAGGATGA CTATGGCTTT TCAGGTTCTC AGAAATTGGA AGGAGAGTTC
    AAACAACCAA TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA
    ACTCAAGTTG TTCATTCTGC GGGAAGGCGG TTTATAGTGA GTCCTGTGCC AGAAAGCCGA
    TTACGAGAAT CAAAAGTTTT CCCCAGTGAA ATAACAGATA CAGTTGCTGC CTCTACAGCT
    CAGAGCCCTG GAATGAACTT GTCTCACTCT GCATCATCCC TTAGTCTACA ACAGGCCTTT
    TCTGAACTTA GACGTGCCCA AATGACAGAA GGACCCAACA CAGCACCTCC AAACTTTAGT
    CATACAGGAC CAACATTTCC AGTAGTACCT CCTTTCTTAA GTAGCATTGC TGGAGTCCCA
    ACCACAGCAG CAGCCACAGC ACCAGTCCCT GCAACAAGCA GCCCTCCTAA TGACATTTCC
    ACATCAGTAA TTCAGTCTGA GGTTACAGTG CCCACTGAAG AGGGGATTGC TGGAGTTGCC
    ACCAGCACAG GTGTGGTAAC TTCAGGTGGT CTCCCCATAC CACCTGTGTC TGAATCACCA
    GTACTTTCCA GCGTAGTTTC AAGTATCACA ATACCTGCAG TTGTCTCAAT ATCTACTACA
    TCCCCGTCAC TTCAAGTCCC CACATCCACA TCTGAGATCG TTGTTTCTAG TACAGCACTG
    TATCCTTCAG TAACAGTTTC AGCAACTTCA GCCTCTGCAG GGGGCAGTAC TGCTACCCCA
    GGTCCTAAGC CTCCAGCTGT AGTATCTCAG CAGGCAGCAG GCAGCACTAC TGTGGGAGCC
    ACATTAACAT CAGTTTCTAC CACCACTTCA TTCCCAAGCA CAGCTTCACA GCTGTGCATT
    CAGCTTAGCA GCAGTACTTC TACTCCTACT TTAGCTGAAA CCGTGGTAGT TAGCGCACAC
    TCACTAGATA AGACATCTCA TAGCAGTACA ACTGGATTGG CTTTCTCCCT CTCTGCACCA
    TCTTCCTCTT CCTCTCCTGG AGCAGGAGTG TCTAGTTATA TTTCTCAGCC TGGTGGGCTG
    CATCCTTTGG TCATTCCATC AGTGATAGCT TCTACTCCTA TTCTTCCCCA AGCAGCAGGA
    CCTACTTCTA CACCTTTATT ACCCCAAGTA CCTAGTATCC CACCCTTGGT ACAGCCTGTT
    GCCAATGTGC CTGCTGTACA GCAGACACTA ATTCATAGTC AGCCTCAACC AGCTTTGCTT
    CCCAACCAGC CCCATACTCA TTGTCCTGAA GTAGATTCTG ATACACAACC CAAAGCTCCT
    GGAATTGATG ACATAAAGAC TCTAGAAGAA AAGCTGCGGT CTCTGTTCAG TGAACACAGC
    TCATCTGGAG CTCAGCATGC CTCTGTCTCA CTGGAGACCT CACTAGTCAT AGAGAGCACT
    GTCACACCAG GCATCCCAAC TACTGCTGTT GCACCAAGCA AACTCCTGAC TTCTACCACA
    AGTACTTGCT TACCACCAAC CAATTTACCA CTAGGAACAG TTGCTTTGCC AGTTACACCA
    GTGGTCACAC CTGGGCAAGT TTCTACCCCA GTCAGCACTA CTACATCAGG AGTGAAACCT
    GGAACTGCTC CCTCCAAGCC ACCTCTAACT AAGGCTCCGG TGCTGCCAGT GGGTACTGAA
    CTTCCAGCAG GTACTCTACC CAGCGAGCAG CTGCCACCTT TTCCAGGACC TTCTCTAACC
    CAGTCCCAGC AACCTCTAGA GGATCTTGAT GCTCAATTGA GAAGAACACT TAGTCCAGAG
    ATGATCACAG TGACTTCTGC GGTTGGTCCT GTGTCCATGG CGGCTCCAAC AGCAATCACA
    GAAGCAGGAA CACAGCCTCA GAAGGGTGTT TCTCAAGTCA AAGAAGGCCC TGTCCTAGCA
    ACTAGTTCAG GAGCTGGTGT TTTTAAGATG GGACGATTTC AGGTTTCTGT TGCAGCAGAC
    GGTGCCCAGA AAGAGGGTAA AAATAAGTCA GAAGATGCAA AGTCTGTTCA TTTTGAATCC
    AGCACCTCAG AGTCCTCAGT GCTATCAAGT AGTAGTCCAG AGAGTACCTT GGTGAAACCA
    GAGCCGAATG GCATAACCAT CCCTGGTATC TCTTCAGATG TGCCAGAGAG TGCCCACAAA
    ACTACTGCCT CAGAGGCAAA GTCAGACACT GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG
    GTGACAACTA CAGCAAACAA AGTGGGTCGT TTCTCTGTAT CAAAAACTGA GGACAAGATC
    ACTGACACAA AGAAAGAAGG ACCAGTGGCA TCTCCTCCTT TTATGGATTT GGAACAAGCT
    GTTCTTCCTG CTGTGATACC AAAGAAAGAG AAGCCTGAAC TGTCAGAGCC TTCACATCTA
    AATGGGCCGT CTTCTGACCC GGAGGCCGCT TTTTTAAGTA GGGATGTGGA TGATGGTTCC
    GGTAGTCCAC ACTCGCCCCA TCAGCTGAGC TCAAAGAGCC TTCCTAGCCA GAATCTAAGT
    CAAAGCCTTA GTAATTCATT TAACTCCTCT TACATGAGTA GCGACAATGA GTCAGATATC
    GAAGATGAAG ACTTAAAGTT AGAGCTGCGA CGACTACGAG ATAAACATCT CAAAGAGATT
    CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACCAA ACTGGGCAAG
    GTGCCCCCTG CTGTTATTAT TCCCCCAGCT GCTCCCCTTT CAGGGAGAAG ACGACGACCC
    ACTAAAAGCA AAGGCAGCAA ATCTAGTCGA AGCAGTTCCT TGGGGAATAA AAGCCCCCAG
    CTTTCAGGTA ACCTGTCTGG TCAGAGTGCA GCTTCAGTCT TGCACCCCCA GCAGACCCTC
    CACCCTCCTG GCAACATCCC AGAGTCCGGG CAGAATCAGC TGTTACAGCC CCTTAAGCCA
    TCTCCCTCCA GTGACAACCT CTATTCAGCC TTCACCAGTG ATGGTGCCAT TTCAGTACCA
    AGCCTTTCTG CTCCAGGTCA AGGAACCAGC AGCACAAACA CTGTTGGGGC AACAGTGAAC
    AGCCAAGCCG CCCAAGCTCA GCCTCCTGCC ATGACGTCCA GCAGGAAGGG CACATTCACA
    GATGACTTGC ACAAGTTGGT AGACAATTGG GCCCGAGATG CCATGAATCT CTCAGGCAGG
    AGAGGAAGCA AAGGGCACAT GAATTACGAG GGCCCTGGAA TGGCAAGGAA GTTCTCTGCA
    CCTGGGCAAC TGTGCATCTC CATGACCTCG AACCTGGGTG GCTCTGCCCC CATCTCTGCA
    GCATCAGCTA CCTCTCTAGG TCACTTCACC AAGTCTATGT GCCCCCCACA GCAGTATGGC
    TTTCCAGCTA CCCCATTTGG CGCTCAATGG AGTGGGACGG GTGGCCCAGC ACCACAGCCA
    CTTGGCCAGT TCCAACCTGT GGGAACTGCC TCCTTGCAGA ATTTCAACAT CAGCAATTTG
    CAGAAATCCA TCAGCAACCC CCCAGGCTCC AACCTGCGGA CCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu84079
    Clone ID Related Accession (Same CDS sequence) XM_017019839.1
    Accession Version XM_017019839.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6132bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase WNK1 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGCAGGGAAT ACAGCAGACA
    GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC
    ACTACTGCAC AGCCAGTGAG TCAGCCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT
    GGAAAACAGA GTACTCAGGG AGTCTCTCAG GTTGCTCCTG CAGAGCCAGT TGCAGTAGCA
    CAGACCCAAG CTACCCAGCC GACCACTTTG GCTTCCTCTG TAGACAGTGC ACATTCAGAT
    GTTGCTTCAG GTATGAGTGA TGGCAATGAG AACGTCCCAT CTTCCAGTGG AAGGCATGAA
    GGAAGAACTA CAAAACGGCA TTACCGAAAA TCTGTAAGGA GTCGCTCTCG ACATGAAAAA
    ACTTCACGCC CAAAATTAAG AATTTTGAAT GTTTCAAATA AAGGAGACCG AGTAGTAGAA
    TGTCAATTAG AGACTCATAA TAGGAAAATG GTTACATTCA AATTTGACCT AGATGGTGAC
    AACCCCGAGG AGATAGCAAC AATTATGGTG AACAATGACT TTATTCTAGC AATAGAGAGA
    GAGTCGTTTG TGGATCAAGT GCGAGAAATT ATTGAAAAAG CTGATGAAAT GCTCAGTGAG
    GATGTCAGTG TGGAACCAGA GGGTGATCAG GGATTGGAGA GTCTACAAGG AAAGGATGAC
    TATGGCTTTT CAGGTTCTCA GAAATTGGAA GGAGAGTTCA AACAACCAAT TCCTGCGTCT
    TCCATGCCAC AGCAAATAGG CATTCCTACC AGTTCTTTAA CTCAAGTTGT TCATTCTGCG
    GGAAGGCGGT TTATAGTGAG TCCTGTGCCA GAAAGCCGAT TACGAGAATC AAAAGTTTTC
    CCCAGTGAAA TAACAGATAC AGTTGCTGCC TCTACAGCTC AGAGCCCTGG AATGAACTTG
    TCTCACTCTG CATCATCCCT TAGTCTACAA CAGGCCTTTT CTGAACTTAG ACGTGCCCAA
    ATGACAGAAG GACCCAACAC AGCACCTCCA AACTTTAGTC ATACAGGACC AACATTTCCA
    GTAGTACCTC CTTTCTTAAG TAGCATTGCT GGAGTCCCAA CCACAGCAGC AGCCACAGCA
    CCAGTCCCTG CAACAAGCAG CCCTCCTAAT GACATTTCCA CATCAGTAAT TCAGTCTGAG
    GTTACAGTGC CCACTGAAGA GGGGATTGCT GGAGTTGCCA CCAGCACAGG TGTGGTAACT
    TCAGGTGGTC TCCCCATACC ACCTGTGTCT GAATCACCAG TACTTTCCAG CGTAGTTTCA
    AGTATCACAA TACCTGCAGT TGTCTCAATA TCTACTACAT CCCCGTCACT TCAAGTCCCC
    ACATCCACAT CTGAGATCGT TGTTTCTAGT ACAGCACTGT ATCCTTCAGT AACAGTTTCA
    GCAACTTCAG CCTCTGCAGG GGGCAGTACT GCTACCCCAG GTCCTAAGCC TCCAGCTGTA
    GTATCTCAGC AGGCAGCAGG CAGCACTACT GTGGGAGCCA CATTAACATC AGTTTCTACC
    ACCACTTCAT TCCCAAGCAC AGCTTCACAG CTGTGCATTC AGCTTAGCAG CAGTACTTCT
    ACTCCTACTT TAGCTGAAAC CGTGGTAGTT AGCGCACACT CACTAGATAA GACATCTCAT
    AGCAGTACAA CTGGATTGGC TTTCTCCCTC TCTGCACCAT CTTCCTCTTC CTCTCCTGGA
    GCAGGAGTGT CTAGTTATAT TTCTCAGCCT GGTGGGCTGC ATCCTTTGGT CATTCCATCA
    GTGATAGCTT CTACTCCTAT TCTTCCCCAA GCAGCAGGAC CTACTTCTAC ACCTTTATTA
    CCCCAAGTAC CTAGTATCCC ACCCTTGGTA CAGCCTGTTG CCAATGTGCC TGCTGTACAG
    CAGACACTAA TTCATAGTCA GCCTCAACCA GCTTTGCTTC CCAACCAGCC CCATACTCAT
    TGTCCTGAAG TAGATTCTGA TACACAACCC AAAGCTCCTG GAATTGATGA CATAAAGACT
    CTAGAAGAAA AGCTGCGGTC TCTGTTCAGT GAACACAGCT CATCTGGAGC TCAGCATGCC
    TCTGTCTCAC TGGAGACCTC ACTAGTCATA GAGAGCACTG TCACACCAGG CATCCCAACT
    ACTGCTGTTG CACCAAGCAA ACTCCTGACT TCTACCACAA GTACTTGCTT ACCACCAACC
    AATTTACCAC TAGGAACAGT TGCTTTGCCA GTTACACCAG TGGTCACACC TGGGCAAGTT
    TCTACCCCAG TCAGCACTAC TACATCAGGA GTGAAACCTG GAACTGCTCC CTCCAAGCCA
    CCTCTAACTA AGGCTCCGGT GCTGCCAGTG GGTACTGAAC TTCCAGCAGG TACTCTACCC
    AGCGAGCAGC TGCCACCTTT TCCAGGACCT TCTCTAACCC AGTCCCAGCA ACCTCTAGAG
    GATCTTGATG CTCAATTGAG AAGAACACTT AGTCCAGAGA TGATCACAGT GACTTCTGCG
    GTTGGTCCTG TGTCCATGGC GGCTCCAACA GCAATCACAG AAGCAGGAAC ACAGCCTCAG
    AAGGGTGTTT CTCAAGTCAA AGAAGGCCCT GTCCTAGCAA CTAGTTCAGG AGCTGGTGTT
    TTTAAGATGG GACGATTTCA GGTTTCTGTT GCAGCAGACG GTGCCCAGAA AGAGGGTAAA
    AATAAGTCAG AAGATGCAAA GTCTGTTCAT TTTGAATCCA GCACCTCAGA GTCCTCAGTG
    CTATCAAGTA GTAGTCCAGA GAGTACCTTG GTGAAACCAG AGCCGAATGG CATAACCATC
    CCTGGTATCT CTTCAGATGT GCCAGAGAGT GCCCACAAAA CTACTGCCTC AGAGGCAAAG
    TCAGACACTG GGCAGCCTAC CAAGGTTGGA CGTTTTCAGG TGACAACTAC AGCAAACAAA
    GTGGGTCGTT TCTCTGTATC AAAAACTGAG GACAAGATCA CTGACACAAA GAAAGAAGGA
    CCAGTGGCAT CTCCTCCTTT TATGGATTTG GAACAAGCTG TTCTTCCTGC TGTGATACCA
    AAGAAAGAGA AGCCTGAACT GTCAGAGCCT TCACATCTAA ATGGGCCGTC TTCTGACCCG
    GAGGCCGCTT TTTTAAGTAG GGATGTGGAT GATGGTTCCG GTAGTCCACA CTCGCCCCAT
    CAGCTGAGCT CAAAGAGCCT TCCTAGCCAG AATCTAAGTC AAAGCCTTAG TAATTCATTT
    AACTCCTCTT ACATGAGTAG CGACAATGAG TCAGATATCG AAGATGAAGA CTTAAAGTTA
    GAGCTGCGAC GACTACGAGA TAAACATCTC AAAGAGATTC AGGACCTGCA GAGTCGCCAG
    AAGCATGAAA TTGAATCTTT GTATACCAAA CTGGGCAAGG TGCCCCCTGC TGTTATTATT
    CCCCCAGCTG CTCCCCTTTC AGGGAGAAGA CGACGACCCA CTAAAAGCAA AGGCAGCAAA
    TCTAGTCGAA GCAGTTCCTT GGGGAATAAA AGCCCCCAGC TTTCAGGAAC CAGCAGCACA
    AACACTGTTG GGGCAACAGT GAACAGCCAA GCCGCCCAAG CTCAGCCTCC TGCCATGACG
    TCCAGCAGGA AGGGCACATT CACAGATGAC TTGCACAAGT TGGTAGACAA TTGGGCCCGA
    GATGCCATGA ATCTCTCAGG CAGGAGAGGA AGCAAAGGGC ACATGAATTA CGAGGGCCCT
    GGAATGGCAA GGAAGTTCTC TGCACCTGGG CAACTGTGCA TCTCCATGAC CTCGAACCTG
    GGTGGCTCTG CCCCCATCTC TGCAGCATCA GCTACCTCTC TAGGTCACTT CACCAAGTCT
    ATGTGCCCCC CACAGCAGTA TGGCTTTCCA GCTACCCCAT TTGGCGCTCA ATGGAGTGGG
    ACGGGTGGCC CAGCACCACA GCCACTTGGC CAGTTCCAAC CTGTGGGAAC TGCCTCCTTG
    CAGAATTTCA ACATCAGCAA TTTGCAGAAA TCCATCAGCA ACCCCCCAGG CTCCAACCTG
    CGGACCACTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016875328.1
    CDS969..7100
    Translation

    Target ORF information:

    RefSeq Version XM_017019839.1
    Organism Homo sapiens(human)
    Definition Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017019839.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGCAGGGAAT ACAGCAGACA
    GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC
    ACTACTGCAC AGCCAGTGAG TCAGCCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT
    GGAAAACAGA GTACTCAGGG AGTCTCTCAG GTTGCTCCTG CAGAGCCAGT TGCAGTAGCA
    CAGACCCAAG CTACCCAGCC GACCACTTTG GCTTCCTCTG TAGACAGTGC ACATTCAGAT
    GTTGCTTCAG GTATGAGTGA TGGCAATGAG AACGTCCCAT CTTCCAGTGG AAGGCATGAA
    GGAAGAACTA CAAAACGGCA TTACCGAAAA TCTGTAAGGA GTCGCTCTCG ACATGAAAAA
    ACTTCACGCC CAAAATTAAG AATTTTGAAT GTTTCAAATA AAGGAGACCG AGTAGTAGAA
    TGTCAATTAG AGACTCATAA TAGGAAAATG GTTACATTCA AATTTGACCT AGATGGTGAC
    AACCCCGAGG AGATAGCAAC AATTATGGTG AACAATGACT TTATTCTAGC AATAGAGAGA
    GAGTCGTTTG TGGATCAAGT GCGAGAAATT ATTGAAAAAG CTGATGAAAT GCTCAGTGAG
    GATGTCAGTG TGGAACCAGA GGGTGATCAG GGATTGGAGA GTCTACAAGG AAAGGATGAC
    TATGGCTTTT CAGGTTCTCA GAAATTGGAA GGAGAGTTCA AACAACCAAT TCCTGCGTCT
    TCCATGCCAC AGCAAATAGG CATTCCTACC AGTTCTTTAA CTCAAGTTGT TCATTCTGCG
    GGAAGGCGGT TTATAGTGAG TCCTGTGCCA GAAAGCCGAT TACGAGAATC AAAAGTTTTC
    CCCAGTGAAA TAACAGATAC AGTTGCTGCC TCTACAGCTC AGAGCCCTGG AATGAACTTG
    TCTCACTCTG CATCATCCCT TAGTCTACAA CAGGCCTTTT CTGAACTTAG ACGTGCCCAA
    ATGACAGAAG GACCCAACAC AGCACCTCCA AACTTTAGTC ATACAGGACC AACATTTCCA
    GTAGTACCTC CTTTCTTAAG TAGCATTGCT GGAGTCCCAA CCACAGCAGC AGCCACAGCA
    CCAGTCCCTG CAACAAGCAG CCCTCCTAAT GACATTTCCA CATCAGTAAT TCAGTCTGAG
    GTTACAGTGC CCACTGAAGA GGGGATTGCT GGAGTTGCCA CCAGCACAGG TGTGGTAACT
    TCAGGTGGTC TCCCCATACC ACCTGTGTCT GAATCACCAG TACTTTCCAG CGTAGTTTCA
    AGTATCACAA TACCTGCAGT TGTCTCAATA TCTACTACAT CCCCGTCACT TCAAGTCCCC
    ACATCCACAT CTGAGATCGT TGTTTCTAGT ACAGCACTGT ATCCTTCAGT AACAGTTTCA
    GCAACTTCAG CCTCTGCAGG GGGCAGTACT GCTACCCCAG GTCCTAAGCC TCCAGCTGTA
    GTATCTCAGC AGGCAGCAGG CAGCACTACT GTGGGAGCCA CATTAACATC AGTTTCTACC
    ACCACTTCAT TCCCAAGCAC AGCTTCACAG CTGTGCATTC AGCTTAGCAG CAGTACTTCT
    ACTCCTACTT TAGCTGAAAC CGTGGTAGTT AGCGCACACT CACTAGATAA GACATCTCAT
    AGCAGTACAA CTGGATTGGC TTTCTCCCTC TCTGCACCAT CTTCCTCTTC CTCTCCTGGA
    GCAGGAGTGT CTAGTTATAT TTCTCAGCCT GGTGGGCTGC ATCCTTTGGT CATTCCATCA
    GTGATAGCTT CTACTCCTAT TCTTCCCCAA GCAGCAGGAC CTACTTCTAC ACCTTTATTA
    CCCCAAGTAC CTAGTATCCC ACCCTTGGTA CAGCCTGTTG CCAATGTGCC TGCTGTACAG
    CAGACACTAA TTCATAGTCA GCCTCAACCA GCTTTGCTTC CCAACCAGCC CCATACTCAT
    TGTCCTGAAG TAGATTCTGA TACACAACCC AAAGCTCCTG GAATTGATGA CATAAAGACT
    CTAGAAGAAA AGCTGCGGTC TCTGTTCAGT GAACACAGCT CATCTGGAGC TCAGCATGCC
    TCTGTCTCAC TGGAGACCTC ACTAGTCATA GAGAGCACTG TCACACCAGG CATCCCAACT
    ACTGCTGTTG CACCAAGCAA ACTCCTGACT TCTACCACAA GTACTTGCTT ACCACCAACC
    AATTTACCAC TAGGAACAGT TGCTTTGCCA GTTACACCAG TGGTCACACC TGGGCAAGTT
    TCTACCCCAG TCAGCACTAC TACATCAGGA GTGAAACCTG GAACTGCTCC CTCCAAGCCA
    CCTCTAACTA AGGCTCCGGT GCTGCCAGTG GGTACTGAAC TTCCAGCAGG TACTCTACCC
    AGCGAGCAGC TGCCACCTTT TCCAGGACCT TCTCTAACCC AGTCCCAGCA ACCTCTAGAG
    GATCTTGATG CTCAATTGAG AAGAACACTT AGTCCAGAGA TGATCACAGT GACTTCTGCG
    GTTGGTCCTG TGTCCATGGC GGCTCCAACA GCAATCACAG AAGCAGGAAC ACAGCCTCAG
    AAGGGTGTTT CTCAAGTCAA AGAAGGCCCT GTCCTAGCAA CTAGTTCAGG AGCTGGTGTT
    TTTAAGATGG GACGATTTCA GGTTTCTGTT GCAGCAGACG GTGCCCAGAA AGAGGGTAAA
    AATAAGTCAG AAGATGCAAA GTCTGTTCAT TTTGAATCCA GCACCTCAGA GTCCTCAGTG
    CTATCAAGTA GTAGTCCAGA GAGTACCTTG GTGAAACCAG AGCCGAATGG CATAACCATC
    CCTGGTATCT CTTCAGATGT GCCAGAGAGT GCCCACAAAA CTACTGCCTC AGAGGCAAAG
    TCAGACACTG GGCAGCCTAC CAAGGTTGGA CGTTTTCAGG TGACAACTAC AGCAAACAAA
    GTGGGTCGTT TCTCTGTATC AAAAACTGAG GACAAGATCA CTGACACAAA GAAAGAAGGA
    CCAGTGGCAT CTCCTCCTTT TATGGATTTG GAACAAGCTG TTCTTCCTGC TGTGATACCA
    AAGAAAGAGA AGCCTGAACT GTCAGAGCCT TCACATCTAA ATGGGCCGTC TTCTGACCCG
    GAGGCCGCTT TTTTAAGTAG GGATGTGGAT GATGGTTCCG GTAGTCCACA CTCGCCCCAT
    CAGCTGAGCT CAAAGAGCCT TCCTAGCCAG AATCTAAGTC AAAGCCTTAG TAATTCATTT
    AACTCCTCTT ACATGAGTAG CGACAATGAG TCAGATATCG AAGATGAAGA CTTAAAGTTA
    GAGCTGCGAC GACTACGAGA TAAACATCTC AAAGAGATTC AGGACCTGCA GAGTCGCCAG
    AAGCATGAAA TTGAATCTTT GTATACCAAA CTGGGCAAGG TGCCCCCTGC TGTTATTATT
    CCCCCAGCTG CTCCCCTTTC AGGGAGAAGA CGACGACCCA CTAAAAGCAA AGGCAGCAAA
    TCTAGTCGAA GCAGTTCCTT GGGGAATAAA AGCCCCCAGC TTTCAGGAAC CAGCAGCACA
    AACACTGTTG GGGCAACAGT GAACAGCCAA GCCGCCCAAG CTCAGCCTCC TGCCATGACG
    TCCAGCAGGA AGGGCACATT CACAGATGAC TTGCACAAGT TGGTAGACAA TTGGGCCCGA
    GATGCCATGA ATCTCTCAGG CAGGAGAGGA AGCAAAGGGC ACATGAATTA CGAGGGCCCT
    GGAATGGCAA GGAAGTTCTC TGCACCTGGG CAACTGTGCA TCTCCATGAC CTCGAACCTG
    GGTGGCTCTG CCCCCATCTC TGCAGCATCA GCTACCTCTC TAGGTCACTT CACCAAGTCT
    ATGTGCCCCC CACAGCAGTA TGGCTTTCCA GCTACCCCAT TTGGCGCTCA ATGGAGTGGG
    ACGGGTGGCC CAGCACCACA GCCACTTGGC CAGTTCCAAC CTGTGGGAAC TGCCTCCTTG
    CAGAATTTCA ACATCAGCAA TTTGCAGAAA TCCATCAGCA ACCCCCCAGG CTCCAACCTG
    CGGACCACTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28095
    Clone ID Related Accession (Same CDS sequence) NM_213655.4
    Accession Version NM_213655.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7905bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-20
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase WNK1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004765.2 and AC004803.11. This sequence is a reference standard in the RefSeqGene project. On Jul 17, 2010 this sequence version replaced NM_213655.3. Transcript Variant: This variant (3) has multiple differences in the coding region but maintains the reading frame compared to variant 1. This variant represents the exon combination of the brain and spinal cord variant described in Figure 2F of PubMed ID 18521183. This variant encodes isoform 3, which is longer than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. The combination of alternatively spliced exons within the coding region is inferred based on experimental evidence reported in Figures 2F and 3 from PubMed ID 18521183. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2156670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCGTCGTGG CCGTAGCATG
    TCGGTTTGTG TTCCCATCTT TCTGCTGTTG CCTCTGTGTC CCGCATCTCT CCCAGTGCTC
    TTCCACCCCA CCGCCAGTAC TGTCTGCACC TCTTTCTCCT TCCCTCCTCC GGACTGCCCC
    GAGGAAACTT TTGCCGAAAA GCTTTCTAAA GCATTGGAGA GTGTCCTGCC TATGCACTCT
    GCCTCTCAGC GCAAGCACCG ACGCTCCAGC CTGCCTTCCC TCTTTGTCAG TACTCCTCAG
    TCCATGGCGC ATCCGTGTGG GGGGACCCCA ACATACCCAG AATCACAGAT ATTTTTCCCA
    ACTATTCATG AACGTCCAGT TTCTTTTTCA CCACCTCCCA CCTGCCCACC GAAAGTAGCC
    ATTTCCCAGC GGCGTAAGAG CACCTCCTTC CTGGAAGCCC AAACTCACCA CTTCCAACCC
    CTGCTGAGGA CTGTTGGCCA AAGTCTTCTT CCACCTGGTG GCAGCCCAAC TAACTGGACA
    CCAGAGGCCG TAGTTATGTT GGGTACTACA GCCAGTAGAG TAACTGGAGA GTCATGTGAG
    ATACAGGTCC ATCCTATGTT TGAACCATCT CAAGTTTACA GTGACTATAG ACCTGGACTA
    GTACTTCCAG AAGAAGCTCA CTATTTTATT CCTCAGGAAG CAGTGTATGT AGCTGGGGTA
    CATTACCAGG CCCGGGTGGC AGAACAGTAT GAGGGCATTC CATACAACTC ATCAGTACTG
    TCAAGTCCTA TGAAACAGAT ACCTGAACAG AAGCCAGTAC AAGGGGGCCC TACTTCAAGT
    TCTGTCTTTG AATTTCCATC TGGACAGGCT TTCCTGGTAG GACACCTTCA GAATCTAAGA
    TTAGATTCTG GATTGGGTCC GGGATCTCCC CTCTCTAGTA TTTCTGCACC TATCAGTACA
    GATGCTACAC GTTTGAAATT TCACCCTGTC TTTGTTCCTC ATTCTGCGCC TGCTGTGTTA
    ACTCATAACA ATGAGAGCAG AAGCAACTGT GTATTTGAAT TTCATGTTCA CACACCAAGC
    TCCTCTTCAG GAGAAGGAGG TGGAATTTTA CCTCAGCGTG TTTACCGAAA TCGGCAGGTT
    GCAGTGGACT TGAATCAAGA AGAACTGCCT CCTCAATCAG TTGGATTACA TGGCTACTTG
    CAGCCTGTGA CTGAAGAAAA GCATAATTAC CATGCCCCAG AATTGACCGT TTCTGTGGTA
    GAGCCTATCG GACAGAACTG GCCAATAGGA AGCCCAGAAT ATTCCAGTGA TTCCTCACAA
    ATCACTTCTT CAGACCCCAG TGATTTTCAG TCACCTCCCC CTACAGGGGG AGCAGCTGCA
    CCTTTTGGCT CTGACGTCTC AATGCCCTTT ATCCATCTGC CTCAGACAGT GTTACAAGAA
    TCCCCACTTT TCTTCTGTTT CCCCCAAGGA ACCACATCTC AGCAGGTCTT AACTGCCTCA
    TTTTCTTCAG GAGGATCTGC ACTTCATCCA CAGGCACAGG GGCAGAGCCA GGGTCAGCCA
    TCCTCAAGTA GCTTAACAGG GGTTTCATCT TCCCAACCCA TACAACATCC TCAGCAGCAG
    CAGGGAATAC AGCAGACAGC CCCTCCTCAA CAGACAGTGC AGTATTCACT TTCACAGACA
    TCAACCTCCA GTGAGGCCAC TACTGCACAG CCAGTGAGTC AGCCTCAAGC TCCACAAGTC
    TTGCCTCAAG TATCAGCTGG AAAACAGAGT ACTCAGGGAG TCTCTCAGGT TGCTCCTGCA
    GAGCCAGTTG CAGTAGCACA GACCCAAGCT ACCCAGCCGA CCACTTTGGC TTCCTCTGTA
    GACAGTGCAC ATTCAGATGT TGCTTCAGGT ATGAGTGATG GCAATGAGAA CGTCCCATCT
    TCCAGTGGAA GGCATGAAGG AAGAACTACA AAACGGCATT ACCGAAAATC TGTAAGGAGT
    CGCTCTCGAC ATGAAAAAAC TTCACGCCCA AAATTAAGAA TTTTGAATGT TTCAAATAAA
    GGAGACCGAG TAGTAGAATG TCAATTAGAG ACTCATAATA GGAAAATGGT TACATTCAAA
    TTTGACCTAG ATGGTGACAA CCCCGAGGAG ATAGCAACAA TTATGGTGAA CAATGACTTT
    ATTCTAGCAA TAGAGAGAGA GTCGTTTGTG GATCAAGTGC GAGAAATTAT TGAAAAAGCT
    GATGAAATGC TCAGTGAGGA TGTCAGTGTG GAACCAGAGG GTGATCAGGG ATTGGAGAGT
    CTACAAGGAA AGGATGACTA TGGCTTTTCA GGTTCTCAGA AATTGGAAGG AGAGTTCAAA
    CAACCAATTC CTGCGTCTTC CATGCCACAG CAAATAGGCA TTCCTACCAG TTCTTTAACT
    CAAGTTGTTC ATTCTGCGGG AAGGCGGTTT ATAGTGAGTC CTGTGCCAGA AAGCCGATTA
    CGAGAATCAA AAGTTTTCCC CAGTGAAATA ACAGATACAG TTGCTGCCTC TACAGCTCAG
    AGCCCTGGAA TGAACTTGTC TCACTCTGCA TCATCCCTTA GTCTACAACA GGCCTTTTCT
    GAACTTAGAC GTGCCCAAAT GACAGAAGGA CCCAACACAG CACCTCCAAA CTTTAGTCAT
    ACAGGACCAA CATTTCCAGT AGTACCTCCT TTCTTAAGTA GCATTGCTGG AGTCCCAACC
    ACAGCAGCAG CCACAGCACC AGTCCCTGCA ACAAGCAGCC CTCCTAATGA CATTTCCACA
    TCAGTAATTC AGTCTGAGGT TACAGTGCCC ACTGAAGAGG GGATTGCTGG AGTTGCCACC
    AGCACAGGTG TGGTAACTTC AGGTGGTCTC CCCATACCAC CTGTGTCTGA ATCACCAGTA
    CTTTCCAGCG TAGTTTCAAG TATCACAATA CCTGCAGTTG TCTCAATATC TACTACATCC
    CCGTCACTTC AAGTCCCCAC ATCCACATCT GAGATCGTTG TTTCTAGTAC AGCACTGTAT
    CCTTCAGTAA CAGTTTCAGC AACTTCAGCC TCTGCAGGGG GCAGTACTGC TACCCCAGGT
    CCTAAGCCTC CAGCTGTAGT ATCTCAGCAG GCAGCAGGCA GCACTACTGT GGGAGCCACA
    TTAACATCAG TTTCTACCAC CACTTCATTC CCAAGCACAG CTTCACAGCT GTGCATTCAG
    CTTAGCAGCA GTACTTCTAC TCCTACTTTA GCTGAAACCG TGGTAGTTAG CGCACACTCA
    CTAGATAAGA CATCTCATAG CAGTACAACT GGATTGGCTT TCTCCCTCTC TGCACCATCT
    TCCTCTTCCT CTCCTGGAGC AGGAGTGTCT AGTTATATTT CTCAGCCTGG TGGGCTGCAT
    CCTTTGGTCA TTCCATCAGT GATAGCTTCT ACTCCTATTC TTCCCCAAGC AGCAGGACCT
    ACTTCTACAC CTTTATTACC CCAAGTACCT AGTATCCCAC CCTTGGTACA GCCTGTTGCC
    AATGTGCCTG CTGTACAGCA GACACTAATT CATAGTCAGC CTCAACCAGC TTTGCTTCCC
    AACCAGCCCC ATACTCATTG TCCTGAAGTA GATTCTGATA CACAACCCAA AGCTCCTGGA
    ATTGATGACA TAAAGACTCT AGAAGAAAAG CTGCGGTCTC TGTTCAGTGA ACACAGCTCA
    TCTGGAGCTC AGCATGCCTC TGTCTCACTG GAGACCTCAC TAGTCATAGA GAGCACTGTC
    ACACCAGGCA TCCCAACTAC TGCTGTTGCA CCAAGCAAAC TCCTGACTTC TACCACAAGT
    ACTTGCTTAC CACCAACCAA TTTACCACTA GGAACAGTTG CTTTGCCAGT TACACCAGTG
    GTCACACCTG GGCAAGTTTC TACCCCAGTC AGCACTACTA CATCAGGAGT GAAACCTGGA
    ACTGCTCCCT CCAAGCCACC TCTAACTAAG GCTCCGGTGC TGCCAGTGGG TACTGAACTT
    CCAGCAGGTA CTCTACCCAG CGAGCAGCTG CCACCTTTTC CAGGACCTTC TCTAACCCAG
    TCCCAGCAAC CTCTAGAGGA TCTTGATGCT CAATTGAGAA GAACACTTAG TCCAGAGATG
    ATCACAGTGA CTTCTGCGGT TGGTCCTGTG TCCATGGCGG CTCCAACAGC AATCACAGAA
    GCAGGAACAC AGCCTCAGAA GGGTGTTTCT CAAGTCAAAG AAGGCCCTGT CCTAGCAACT
    AGTTCAGGAG CTGGTGTTTT TAAGATGGGA CGATTTCAGG TTTCTGTTGC AGCAGACGGT
    GCCCAGAAAG AGGGTAAAAA TAAGTCAGAA GATGCAAAGT CTGTTCATTT TGAATCCAGC
    ACCTCAGAGT CCTCAGTGCT ATCAAGTAGT AGTCCAGAGA GTACCTTGGT GAAACCAGAG
    CCGAATGGCA TAACCATCCC TGGTATCTCT TCAGATGTGC CAGAGAGTGC CCACAAAACT
    ACTGCCTCAG AGGCAAAGTC AGACACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG
    ACAACTACAG CAAACAAAGT GGGTCGTTTC TCTGTATCAA AAACTGAGGA CAAGATCACT
    GACACAAAGA AAGAAGGACC AGTGGCATCT CCTCCTTTTA TGGATTTGGA ACAAGCTGTT
    CTTCCTGCTG TGATACCAAA GAAAGAGAAG CCTGAACTGT CAGAGCCTTC ACATCTAAAT
    GGGCCGTCTT CTGACCCGGA GGCCGCTTTT TTAAGTAGGG ATGTGGATGA TGGTTCCGGT
    AGTCCACACT CGCCCCATCA GCTGAGCTCA AAGAGCCTTC CTAGCCAGAA TCTAAGTCAA
    AGCCTTAGTA ATTCATTTAA CTCCTCTTAC ATGAGTAGCG ACAATGAGTC AGATATCGAA
    GATGAAGACT TAAAGTTAGA GCTGCGACGA CTACGAGATA AACATCTCAA AGAGATTCAG
    GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGCAAGGTG
    CCCCCTGCTG TTATTATTCC CCCAGCTGCT CCCCTTTCAG GGAGAAGACG ACGACCCACT
    AAAAGCAAAG GCAGCAAATC TAGTCGAAGC AGTTCCTTGG GGAATAAAAG CCCCCAGCTT
    TCAGGTAACC TGTCTGGTCA GAGTGCAGCT TCAGTCTTGC ACCCCCAGCA GACCCTCCAC
    CCTCCTGGCA ACATCCCAGA GTCCGGGCAG AATCAGCTGT TACAGCCCCT TAAGCCATCT
    CCCTCCAGTG ACAACCTCTA TTCAGCCTTC ACCAGTGATG GTGCCATTTC AGTACCAAGC
    CTTTCTGCTC CAGGTCAAGG AACCAGCAGC ACAAACACTG TTGGGGCAAC AGTGAACAGC
    CAAGCCGCCC AAGCTCAGCC TCCTGCCATG ACGTCCAGCA GGAAGGGCAC ATTCACAGAT
    GACTTGCACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGGAGA
    GGAAGCAAAG GGCACATGAA TTACGAGGGC CCTGGAATGG CAAGGAAGTT CTCTGCACCT
    GGGCAACTGT GCATCTCCAT GACCTCGAAC CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA
    TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTATGGCTTT
    CCAGCTACCC CATTTGGCGC TCAATGGAGT GGGACGGGTG GCCCAGCACC ACAGCCACTT
    GGCCAGTTCC AACCTGTGGG AACTGCCTCC TTGCAGAATT TCAACATCAG CAATTTGCAG
    AAATCCATCA GCAACCCCCC AGGCTCCAAC CTGCGGACCA CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_998820.3
    CDS644..8548
    Translation

    Target ORF information:

    RefSeq Version NM_213655.4
    Organism Homo sapiens(human)
    Definition Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_213655.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCGTCGTGG CCGTAGCATG
    TCGGTTTGTG TTCCCATCTT TCTGCTGTTG CCTCTGTGTC CCGCATCTCT CCCAGTGCTC
    TTCCACCCCA CCGCCAGTAC TGTCTGCACC TCTTTCTCCT TCCCTCCTCC GGACTGCCCC
    GAGGAAACTT TTGCCGAAAA GCTTTCTAAA GCATTGGAGA GTGTCCTGCC TATGCACTCT
    GCCTCTCAGC GCAAGCACCG ACGCTCCAGC CTGCCTTCCC TCTTTGTCAG TACTCCTCAG
    TCCATGGCGC ATCCGTGTGG GGGGACCCCA ACATACCCAG AATCACAGAT ATTTTTCCCA
    ACTATTCATG AACGTCCAGT TTCTTTTTCA CCACCTCCCA CCTGCCCACC GAAAGTAGCC
    ATTTCCCAGC GGCGTAAGAG CACCTCCTTC CTGGAAGCCC AAACTCACCA CTTCCAACCC
    CTGCTGAGGA CTGTTGGCCA AAGTCTTCTT CCACCTGGTG GCAGCCCAAC TAACTGGACA
    CCAGAGGCCG TAGTTATGTT GGGTACTACA GCCAGTAGAG TAACTGGAGA GTCATGTGAG
    ATACAGGTCC ATCCTATGTT TGAACCATCT CAAGTTTACA GTGACTATAG ACCTGGACTA
    GTACTTCCAG AAGAAGCTCA CTATTTTATT CCTCAGGAAG CAGTGTATGT AGCTGGGGTA
    CATTACCAGG CCCGGGTGGC AGAACAGTAT GAGGGCATTC CATACAACTC ATCAGTACTG
    TCAAGTCCTA TGAAACAGAT ACCTGAACAG AAGCCAGTAC AAGGGGGCCC TACTTCAAGT
    TCTGTCTTTG AATTTCCATC TGGACAGGCT TTCCTGGTAG GACACCTTCA GAATCTAAGA
    TTAGATTCTG GATTGGGTCC GGGATCTCCC CTCTCTAGTA TTTCTGCACC TATCAGTACA
    GATGCTACAC GTTTGAAATT TCACCCTGTC TTTGTTCCTC ATTCTGCGCC TGCTGTGTTA
    ACTCATAACA ATGAGAGCAG AAGCAACTGT GTATTTGAAT TTCATGTTCA CACACCAAGC
    TCCTCTTCAG GAGAAGGAGG TGGAATTTTA CCTCAGCGTG TTTACCGAAA TCGGCAGGTT
    GCAGTGGACT TGAATCAAGA AGAACTGCCT CCTCAATCAG TTGGATTACA TGGCTACTTG
    CAGCCTGTGA CTGAAGAAAA GCATAATTAC CATGCCCCAG AATTGACCGT TTCTGTGGTA
    GAGCCTATCG GACAGAACTG GCCAATAGGA AGCCCAGAAT ATTCCAGTGA TTCCTCACAA
    ATCACTTCTT CAGACCCCAG TGATTTTCAG TCACCTCCCC CTACAGGGGG AGCAGCTGCA
    CCTTTTGGCT CTGACGTCTC AATGCCCTTT ATCCATCTGC CTCAGACAGT GTTACAAGAA
    TCCCCACTTT TCTTCTGTTT CCCCCAAGGA ACCACATCTC AGCAGGTCTT AACTGCCTCA
    TTTTCTTCAG GAGGATCTGC ACTTCATCCA CAGGCACAGG GGCAGAGCCA GGGTCAGCCA
    TCCTCAAGTA GCTTAACAGG GGTTTCATCT TCCCAACCCA TACAACATCC TCAGCAGCAG
    CAGGGAATAC AGCAGACAGC CCCTCCTCAA CAGACAGTGC AGTATTCACT TTCACAGACA
    TCAACCTCCA GTGAGGCCAC TACTGCACAG CCAGTGAGTC AGCCTCAAGC TCCACAAGTC
    TTGCCTCAAG TATCAGCTGG AAAACAGAGT ACTCAGGGAG TCTCTCAGGT TGCTCCTGCA
    GAGCCAGTTG CAGTAGCACA GACCCAAGCT ACCCAGCCGA CCACTTTGGC TTCCTCTGTA
    GACAGTGCAC ATTCAGATGT TGCTTCAGGT ATGAGTGATG GCAATGAGAA CGTCCCATCT
    TCCAGTGGAA GGCATGAAGG AAGAACTACA AAACGGCATT ACCGAAAATC TGTAAGGAGT
    CGCTCTCGAC ATGAAAAAAC TTCACGCCCA AAATTAAGAA TTTTGAATGT TTCAAATAAA
    GGAGACCGAG TAGTAGAATG TCAATTAGAG ACTCATAATA GGAAAATGGT TACATTCAAA
    TTTGACCTAG ATGGTGACAA CCCCGAGGAG ATAGCAACAA TTATGGTGAA CAATGACTTT
    ATTCTAGCAA TAGAGAGAGA GTCGTTTGTG GATCAAGTGC GAGAAATTAT TGAAAAAGCT
    GATGAAATGC TCAGTGAGGA TGTCAGTGTG GAACCAGAGG GTGATCAGGG ATTGGAGAGT
    CTACAAGGAA AGGATGACTA TGGCTTTTCA GGTTCTCAGA AATTGGAAGG AGAGTTCAAA
    CAACCAATTC CTGCGTCTTC CATGCCACAG CAAATAGGCA TTCCTACCAG TTCTTTAACT
    CAAGTTGTTC ATTCTGCGGG AAGGCGGTTT ATAGTGAGTC CTGTGCCAGA AAGCCGATTA
    CGAGAATCAA AAGTTTTCCC CAGTGAAATA ACAGATACAG TTGCTGCCTC TACAGCTCAG
    AGCCCTGGAA TGAACTTGTC TCACTCTGCA TCATCCCTTA GTCTACAACA GGCCTTTTCT
    GAACTTAGAC GTGCCCAAAT GACAGAAGGA CCCAACACAG CACCTCCAAA CTTTAGTCAT
    ACAGGACCAA CATTTCCAGT AGTACCTCCT TTCTTAAGTA GCATTGCTGG AGTCCCAACC
    ACAGCAGCAG CCACAGCACC AGTCCCTGCA ACAAGCAGCC CTCCTAATGA CATTTCCACA
    TCAGTAATTC AGTCTGAGGT TACAGTGCCC ACTGAAGAGG GGATTGCTGG AGTTGCCACC
    AGCACAGGTG TGGTAACTTC AGGTGGTCTC CCCATACCAC CTGTGTCTGA ATCACCAGTA
    CTTTCCAGCG TAGTTTCAAG TATCACAATA CCTGCAGTTG TCTCAATATC TACTACATCC
    CCGTCACTTC AAGTCCCCAC ATCCACATCT GAGATCGTTG TTTCTAGTAC AGCACTGTAT
    CCTTCAGTAA CAGTTTCAGC AACTTCAGCC TCTGCAGGGG GCAGTACTGC TACCCCAGGT
    CCTAAGCCTC CAGCTGTAGT ATCTCAGCAG GCAGCAGGCA GCACTACTGT GGGAGCCACA
    TTAACATCAG TTTCTACCAC CACTTCATTC CCAAGCACAG CTTCACAGCT GTGCATTCAG
    CTTAGCAGCA GTACTTCTAC TCCTACTTTA GCTGAAACCG TGGTAGTTAG CGCACACTCA
    CTAGATAAGA CATCTCATAG CAGTACAACT GGATTGGCTT TCTCCCTCTC TGCACCATCT
    TCCTCTTCCT CTCCTGGAGC AGGAGTGTCT AGTTATATTT CTCAGCCTGG TGGGCTGCAT
    CCTTTGGTCA TTCCATCAGT GATAGCTTCT ACTCCTATTC TTCCCCAAGC AGCAGGACCT
    ACTTCTACAC CTTTATTACC CCAAGTACCT AGTATCCCAC CCTTGGTACA GCCTGTTGCC
    AATGTGCCTG CTGTACAGCA GACACTAATT CATAGTCAGC CTCAACCAGC TTTGCTTCCC
    AACCAGCCCC ATACTCATTG TCCTGAAGTA GATTCTGATA CACAACCCAA AGCTCCTGGA
    ATTGATGACA TAAAGACTCT AGAAGAAAAG CTGCGGTCTC TGTTCAGTGA ACACAGCTCA
    TCTGGAGCTC AGCATGCCTC TGTCTCACTG GAGACCTCAC TAGTCATAGA GAGCACTGTC
    ACACCAGGCA TCCCAACTAC TGCTGTTGCA CCAAGCAAAC TCCTGACTTC TACCACAAGT
    ACTTGCTTAC CACCAACCAA TTTACCACTA GGAACAGTTG CTTTGCCAGT TACACCAGTG
    GTCACACCTG GGCAAGTTTC TACCCCAGTC AGCACTACTA CATCAGGAGT GAAACCTGGA
    ACTGCTCCCT CCAAGCCACC TCTAACTAAG GCTCCGGTGC TGCCAGTGGG TACTGAACTT
    CCAGCAGGTA CTCTACCCAG CGAGCAGCTG CCACCTTTTC CAGGACCTTC TCTAACCCAG
    TCCCAGCAAC CTCTAGAGGA TCTTGATGCT CAATTGAGAA GAACACTTAG TCCAGAGATG
    ATCACAGTGA CTTCTGCGGT TGGTCCTGTG TCCATGGCGG CTCCAACAGC AATCACAGAA
    GCAGGAACAC AGCCTCAGAA GGGTGTTTCT CAAGTCAAAG AAGGCCCTGT CCTAGCAACT
    AGTTCAGGAG CTGGTGTTTT TAAGATGGGA CGATTTCAGG TTTCTGTTGC AGCAGACGGT
    GCCCAGAAAG AGGGTAAAAA TAAGTCAGAA GATGCAAAGT CTGTTCATTT TGAATCCAGC
    ACCTCAGAGT CCTCAGTGCT ATCAAGTAGT AGTCCAGAGA GTACCTTGGT GAAACCAGAG
    CCGAATGGCA TAACCATCCC TGGTATCTCT TCAGATGTGC CAGAGAGTGC CCACAAAACT
    ACTGCCTCAG AGGCAAAGTC AGACACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG
    ACAACTACAG CAAACAAAGT GGGTCGTTTC TCTGTATCAA AAACTGAGGA CAAGATCACT
    GACACAAAGA AAGAAGGACC AGTGGCATCT CCTCCTTTTA TGGATTTGGA ACAAGCTGTT
    CTTCCTGCTG TGATACCAAA GAAAGAGAAG CCTGAACTGT CAGAGCCTTC ACATCTAAAT
    GGGCCGTCTT CTGACCCGGA GGCCGCTTTT TTAAGTAGGG ATGTGGATGA TGGTTCCGGT
    AGTCCACACT CGCCCCATCA GCTGAGCTCA AAGAGCCTTC CTAGCCAGAA TCTAAGTCAA
    AGCCTTAGTA ATTCATTTAA CTCCTCTTAC ATGAGTAGCG ACAATGAGTC AGATATCGAA
    GATGAAGACT TAAAGTTAGA GCTGCGACGA CTACGAGATA AACATCTCAA AGAGATTCAG
    GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGCAAGGTG
    CCCCCTGCTG TTATTATTCC CCCAGCTGCT CCCCTTTCAG GGAGAAGACG ACGACCCACT
    AAAAGCAAAG GCAGCAAATC TAGTCGAAGC AGTTCCTTGG GGAATAAAAG CCCCCAGCTT
    TCAGGTAACC TGTCTGGTCA GAGTGCAGCT TCAGTCTTGC ACCCCCAGCA GACCCTCCAC
    CCTCCTGGCA ACATCCCAGA GTCCGGGCAG AATCAGCTGT TACAGCCCCT TAAGCCATCT
    CCCTCCAGTG ACAACCTCTA TTCAGCCTTC ACCAGTGATG GTGCCATTTC AGTACCAAGC
    CTTTCTGCTC CAGGTCAAGG AACCAGCAGC ACAAACACTG TTGGGGCAAC AGTGAACAGC
    CAAGCCGCCC AAGCTCAGCC TCCTGCCATG ACGTCCAGCA GGAAGGGCAC ATTCACAGAT
    GACTTGCACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGGAGA
    GGAAGCAAAG GGCACATGAA TTACGAGGGC CCTGGAATGG CAAGGAAGTT CTCTGCACCT
    GGGCAACTGT GCATCTCCAT GACCTCGAAC CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA
    TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTATGGCTTT
    CCAGCTACCC CATTTGGCGC TCAATGGAGT GGGACGGGTG GCCCAGCACC ACAGCCACTT
    GGCCAGTTCC AACCTGTGGG AACTGCCTCC TTGCAGAATT TCAACATCAG CAATTTGCAG
    AAATCCATCA GCAACCCCCC AGGCTCCAAC CTGCGGACCA CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28140
    Clone ID Related Accession (Same CDS sequence) NM_001184985.1
    Accession Version NM_001184985.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7929bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-20
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase WNK1 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004765.2 and AC004803.11. Transcript Variant: This variant (4) has multiple differences in the coding region but maintains the reading frame compared to variant 1. This variant represents the exon combination of the dorsal root ganglia and sciatic nerve variant described in Figure 2F of PubMed ID 18521183. This variant encodes isoform 4, which is longer than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. The combination of alternatively spliced exons within the coding region is inferred based on experimental evidence reported in Figures 2F and 3 from PubMed ID 18521183. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG
    TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT
    CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT
    AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT
    GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT
    ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT
    ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA
    GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG
    GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA
    CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT
    CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG
    GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG
    AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG
    AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA
    GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT
    CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA
    GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG
    AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC
    CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC
    GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC
    TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA
    TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA
    ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGCAGGG AATACAGCAG
    ACAGCCCCTC CTCAACAGAC AGTGCAGTAT TCACTTTCAC AGACATCAAC CTCCAGTGAG
    GCCACTACTG CACAGCCAGT GAGTCAGCCT CAAGCTCCAC AAGTCTTGCC TCAAGTATCA
    GCTGGAAAAC AGGGCTTCCC ACCTCGACTG CCACCACAGT ACCCAGGAGA TTCAAATATT
    GCTCCCTCTT CCAACGTGGC TTCTGTTTGC ATCCATTCTA CAGTCCTATC CCCTCCCATG
    CCGACAGAAG TACTGGCTAC ACCTGGGTAC TTTCCCACAG TGGTGCAGCC TTATGTGGAA
    TCAAATCTTT TAGTTCCTAT GGGTGGTGTA GGAGGACAGG TTCAAGTGTC CCAGCCAGGA
    GGGAGTTTAG CACAAGCCCC CACTACATCC TCCCAGCAAG CAGTTTTGGA GAGTACTCAG
    GGAGTCTCTC AGGTTGCTCC TGCAGAGCCA GTTGCAGTAG CACAGACCCA AGCTACCCAG
    CCGACCACTT TGGCTTCCTC TGTAGACAGT GCACATTCAG ATGTTGCTTC AGGTATGAGT
    GATGGCAATG AGAACGTCCC ATCTTCCAGT GGAAGGCATG AAGGAAGAAC TACAAAACGG
    CATTACCGAA AATCTGTAAG GAGTCGCTCT CGACATGAAA AAACTTCACG CCCAAAATTA
    AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAATT AGAGACTCAT
    AATAGGAAAA TGGTTACATT CAAATTTGAC CTAGATGGTG ACAACCCCGA GGAGATAGCA
    ACAATTATGG TGAACAATGA CTTTATTCTA GCAATAGAGA GAGAGTCGTT TGTGGATCAA
    GTGCGAGAAA TTATTGAAAA AGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA
    GAGGGTGATC AGGGATTGGA GAGTCTACAA GGAAAGGATG ACTATGGCTT TTCAGGTTCT
    CAGAAATTGG AAGGAGAGTT CAAACAACCA ATTCCTGCGT CTTCCATGCC ACAGCAAATA
    GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CGGGAAGGCG GTTTATAGTG
    AGTCCTGTGC CAGAAAGCCG ATTACGAGAA TCAAAAGTTT TCCCCAGTGA AATAACAGAT
    ACAGTTGCTG CCTCTACAGC TCAGAGCCCT GGAATGAACT TGTCTCACTC TGCATCATCC
    CTTAGTCTAC AACAGGCCTT TTCTGAACTT AGACGTGCCC AAATGACAGA AGGACCCAAC
    ACAGCACCTC CAAACTTTAG TCATACAGGA CCAACATTTC CAGTAGTACC TCCTTTCTTA
    AGTAGCATTG CTGGAGTCCC AACCACAGCA GCAGCCACAG CACCAGTCCC TGCAACAAGC
    AGCCCTCCTA ATGACATTTC CACATCAGTA ATTCAGTCTG AGGTTACAGT GCCCACTGAA
    GAGGGGATTG CTGGAGTTGC CACCAGCACA GGTGTGGTAA CTTCAGGTGG TCTCCCCATA
    CCACCTGTGT CTGAATCACC AGTACTTTCC AGCGTAGTTT CAAGTATCAC AATACCTGCA
    GTTGTCTCAA TATCTACTAC ATCCCCGTCA CTTCAAGTCC CCACATCCAC ATCTGAGATC
    GTTGTTTCTA GTACAGCACT GTATCCTTCA GTAACAGTTT CAGCAACTTC AGCCTCTGCA
    GGGGGCAGTA CTGCTACCCC AGGTCCTAAG CCTCCAGCTG TAGTATCTCA GCAGGCAGCA
    GGCAGCACTA CTGTGGGAGC CACATTAACA TCAGTTTCTA CCACCACTTC ATTCCCAAGC
    ACAGCTTCAC AGCTGTGCAT TCAGCTTAGC AGCAGTACTT CTACTCCTAC TTTAGCTGAA
    ACCGTGGTAG TTAGCGCACA CTCACTAGAT AAGACATCTC ATAGCAGTAC AACTGGATTG
    GCTTTCTCCC TCTCTGCACC ATCTTCCTCT TCCTCTCCTG GAGCAGGAGT GTCTAGTTAT
    ATTTCTCAGC CTGGTGGGCT GCATCCTTTG GTCATTCCAT CAGTGATAGC TTCTACTCCT
    ATTCTTCCCC AAGCAGCAGG ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAGTATC
    CCACCCTTGG TACAGCCTGT TGCCAATGTG CCTGCTGTAC AGCAGACACT AATTCATAGT
    CAGCCTCAAC CAGCTTTGCT TCCCAACCAG CCCCATACTC ATTGTCCTGA AGTAGATTCT
    GATACACAAC CCAAAGCTCC TGGAATTGAT GACATAAAGA CTCTAGAAGA AAAGCTGCGG
    TCTCTGTTCA GTGAACACAG CTCATCTGGA GCTCAGCATG CCTCTGTCTC ACTGGAGACC
    TCACTAGTCA TAGAGAGCAC TGTCACACCA GGCATCCCAA CTACTGCTGT TGCACCAAGC
    AAACTCCTGA CTTCTACCAC AAGTACTTGC TTACCACCAA CCAATTTACC ACTAGGAACA
    GTTGCTTTGC CAGTTACACC AGTGGTCACA CCTGGGCAAG TTTCTACCCC AGTCAGCACT
    ACTACATCAG GAGTGAAACC TGGAACTGCT CCCTCCAAGC CACCTCTAAC TAAGGCTCCG
    GTGCTGCCAG TGGGTACTGA ACTTCCAGCA GGTACTCTAC CCAGCGAGCA GCTGCCACCT
    TTTCCAGGAC CTTCTCTAAC CCAGTCCCAG CAACCTCTAG AGGATCTTGA TGCTCAATTG
    AGAAGAACAC TTAGTCCAGA GATGATCACA GTGACTTCTG CGGTTGGTCC TGTGTCCATG
    GCGGCTCCAA CAGCAATCAC AGAAGCAGGA ACACAGCCTC AGAAGGGTGT TTCTCAAGTC
    AAAGAAGGCC CTGTCCTAGC AACTAGTTCA GGAGCTGGTG TTTTTAAGAT GGGACGATTT
    CAGGTTTCTG TTGCAGCAGA CGGTGCCCAG AAAGAGGGTA AAAATAAGTC AGAAGATGCA
    AAGTCTGTTC ATTTTGAATC CAGCACCTCA GAGTCCTCAG TGCTATCAAG TAGTAGTCCA
    GAGAGTACCT TGGTGAAACC AGAGCCGAAT GGCATAACCA TCCCTGGTAT CTCTTCAGAT
    GTGCCAGAGA GTGCCCACAA AACTACTGCC TCAGAGGCAA AGTCAGACAC TGGGCAGCCT
    ACCAAGGTTG GACGTTTTCA GGTGACAACT ACAGCAAACA AAGTGGGTCG TTTCTCTGTA
    TCAAAAACTG AGGACAAGAT CACTGACACA AAGAAAGAAG GACCAGTGGC ATCTCCTCCT
    TTTATGGATT TGGAACAAGC TGTTCTTCCT GCTGTGATAC CAAAGAAAGA GAAGCCTGAA
    CTGTCAGAGC CTTCACATCT AAATGGGCCG TCTTCTGACC CGGAGGCCGC TTTTTTAAGT
    AGGGATGTGG ATGATGGTTC CGGTAGTCCA CACTCGCCCC ATCAGCTGAG CTCAAAGAGC
    CTTCCTAGCC AGAATCTAAG TCAAAGCCTT AGTAATTCAT TTAACTCCTC TTACATGAGT
    AGCGACAATG AGTCAGATAT CGAAGATGAA GACTTAAAGT TAGAGCTGCG ACGACTACGA
    GATAAACATC TCAAAGAGAT TCAGGACCTG CAGAGTCGCC AGAAGCATGA AATTGAATCT
    TTGTATACCA AACTGGGCAA GGTGCCCCCT GCTGTTATTA TTCCCCCAGC TGCTCCCCTT
    TCAGGGAGAA GACGACGACC CACTAAAAGC AAAGGCAGCA AATCTAGTCG AAGCAGTTCC
    TTGGGGAATA AAAGCCCCCA GCTTTCAGGT AACCTGTCTG GTCAGAGTGC AGCTTCAGTC
    TTGCACCCCC AGCAGACCCT CCACCCTCCT GGCAACATCC CAGAGTCCGG GCAGAATCAG
    CTGTTACAGC CCCTTAAGCC ATCTCCCTCC AGTGACAACC TCTATTCAGC CTTCACCAGT
    GATGGTGCCA TTTCAGTACC AAGCCTTTCT GCTCCAGGTC AAGGAACCAG CAGCACAAAC
    ACTGTTGGGG CAACAGTGAA CAGCCAAGCC GCCCAAGCTC AGCCTCCTGC CATGACGTCC
    AGCAGGAAGG GCACATTCAC AGATGACTTG CACAAGTTGG TAGACAATTG GGCCCGAGAT
    GCCATGAATC TCTCAGGCAG GAGAGGAAGC AAAGGGCACA TGAATTACGA GGGCCCTGGA
    ATGGCAAGGA AGTTCTCTGC ACCTGGGCAA CTGTGCATCT CCATGACCTC GAACCTGGGT
    GGCTCTGCCC CCATCTCTGC AGCATCAGCT ACCTCTCTAG GTCACTTCAC CAAGTCTATG
    TGCCCCCCAC AGCAGTATGG CTTTCCAGCT ACCCCATTTG GCGCTCAATG GAGTGGGACG
    GGTGGCCCAG CACCACAGCC ACTTGGCCAG TTCCAACCTG TGGGAACTGC CTCCTTGCAG
    AATTTCAACA TCAGCAATTT GCAGAAATCC ATCAGCAACC CCCCAGGCTC CAACCTGCGG
    ACCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001171914.1
    CDS644..8572
    Translation

    Target ORF information:

    RefSeq Version NM_001184985.1
    Organism Homo sapiens(human)
    Definition Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001184985.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG
    TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT
    CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT
    AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT
    GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT
    ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT
    ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA
    GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG
    GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA
    CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT
    CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG
    GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG
    AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG
    AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA
    GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT
    CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA
    GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG
    AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC
    CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC
    GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC
    TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA
    TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA
    ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGCAGGG AATACAGCAG
    ACAGCCCCTC CTCAACAGAC AGTGCAGTAT TCACTTTCAC AGACATCAAC CTCCAGTGAG
    GCCACTACTG CACAGCCAGT GAGTCAGCCT CAAGCTCCAC AAGTCTTGCC TCAAGTATCA
    GCTGGAAAAC AGGGCTTCCC ACCTCGACTG CCACCACAGT ACCCAGGAGA TTCAAATATT
    GCTCCCTCTT CCAACGTGGC TTCTGTTTGC ATCCATTCTA CAGTCCTATC CCCTCCCATG
    CCGACAGAAG TACTGGCTAC ACCTGGGTAC TTTCCCACAG TGGTGCAGCC TTATGTGGAA
    TCAAATCTTT TAGTTCCTAT GGGTGGTGTA GGAGGACAGG TTCAAGTGTC CCAGCCAGGA
    GGGAGTTTAG CACAAGCCCC CACTACATCC TCCCAGCAAG CAGTTTTGGA GAGTACTCAG
    GGAGTCTCTC AGGTTGCTCC TGCAGAGCCA GTTGCAGTAG CACAGACCCA AGCTACCCAG
    CCGACCACTT TGGCTTCCTC TGTAGACAGT GCACATTCAG ATGTTGCTTC AGGTATGAGT
    GATGGCAATG AGAACGTCCC ATCTTCCAGT GGAAGGCATG AAGGAAGAAC TACAAAACGG
    CATTACCGAA AATCTGTAAG GAGTCGCTCT CGACATGAAA AAACTTCACG CCCAAAATTA
    AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAATT AGAGACTCAT
    AATAGGAAAA TGGTTACATT CAAATTTGAC CTAGATGGTG ACAACCCCGA GGAGATAGCA
    ACAATTATGG TGAACAATGA CTTTATTCTA GCAATAGAGA GAGAGTCGTT TGTGGATCAA
    GTGCGAGAAA TTATTGAAAA AGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA
    GAGGGTGATC AGGGATTGGA GAGTCTACAA GGAAAGGATG ACTATGGCTT TTCAGGTTCT
    CAGAAATTGG AAGGAGAGTT CAAACAACCA ATTCCTGCGT CTTCCATGCC ACAGCAAATA
    GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CGGGAAGGCG GTTTATAGTG
    AGTCCTGTGC CAGAAAGCCG ATTACGAGAA TCAAAAGTTT TCCCCAGTGA AATAACAGAT
    ACAGTTGCTG CCTCTACAGC TCAGAGCCCT GGAATGAACT TGTCTCACTC TGCATCATCC
    CTTAGTCTAC AACAGGCCTT TTCTGAACTT AGACGTGCCC AAATGACAGA AGGACCCAAC
    ACAGCACCTC CAAACTTTAG TCATACAGGA CCAACATTTC CAGTAGTACC TCCTTTCTTA
    AGTAGCATTG CTGGAGTCCC AACCACAGCA GCAGCCACAG CACCAGTCCC TGCAACAAGC
    AGCCCTCCTA ATGACATTTC CACATCAGTA ATTCAGTCTG AGGTTACAGT GCCCACTGAA
    GAGGGGATTG CTGGAGTTGC CACCAGCACA GGTGTGGTAA CTTCAGGTGG TCTCCCCATA
    CCACCTGTGT CTGAATCACC AGTACTTTCC AGCGTAGTTT CAAGTATCAC AATACCTGCA
    GTTGTCTCAA TATCTACTAC ATCCCCGTCA CTTCAAGTCC CCACATCCAC ATCTGAGATC
    GTTGTTTCTA GTACAGCACT GTATCCTTCA GTAACAGTTT CAGCAACTTC AGCCTCTGCA
    GGGGGCAGTA CTGCTACCCC AGGTCCTAAG CCTCCAGCTG TAGTATCTCA GCAGGCAGCA
    GGCAGCACTA CTGTGGGAGC CACATTAACA TCAGTTTCTA CCACCACTTC ATTCCCAAGC
    ACAGCTTCAC AGCTGTGCAT TCAGCTTAGC AGCAGTACTT CTACTCCTAC TTTAGCTGAA
    ACCGTGGTAG TTAGCGCACA CTCACTAGAT AAGACATCTC ATAGCAGTAC AACTGGATTG
    GCTTTCTCCC TCTCTGCACC ATCTTCCTCT TCCTCTCCTG GAGCAGGAGT GTCTAGTTAT
    ATTTCTCAGC CTGGTGGGCT GCATCCTTTG GTCATTCCAT CAGTGATAGC TTCTACTCCT
    ATTCTTCCCC AAGCAGCAGG ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAGTATC
    CCACCCTTGG TACAGCCTGT TGCCAATGTG CCTGCTGTAC AGCAGACACT AATTCATAGT
    CAGCCTCAAC CAGCTTTGCT TCCCAACCAG CCCCATACTC ATTGTCCTGA AGTAGATTCT
    GATACACAAC CCAAAGCTCC TGGAATTGAT GACATAAAGA CTCTAGAAGA AAAGCTGCGG
    TCTCTGTTCA GTGAACACAG CTCATCTGGA GCTCAGCATG CCTCTGTCTC ACTGGAGACC
    TCACTAGTCA TAGAGAGCAC TGTCACACCA GGCATCCCAA CTACTGCTGT TGCACCAAGC
    AAACTCCTGA CTTCTACCAC AAGTACTTGC TTACCACCAA CCAATTTACC ACTAGGAACA
    GTTGCTTTGC CAGTTACACC AGTGGTCACA CCTGGGCAAG TTTCTACCCC AGTCAGCACT
    ACTACATCAG GAGTGAAACC TGGAACTGCT CCCTCCAAGC CACCTCTAAC TAAGGCTCCG
    GTGCTGCCAG TGGGTACTGA ACTTCCAGCA GGTACTCTAC CCAGCGAGCA GCTGCCACCT
    TTTCCAGGAC CTTCTCTAAC CCAGTCCCAG CAACCTCTAG AGGATCTTGA TGCTCAATTG
    AGAAGAACAC TTAGTCCAGA GATGATCACA GTGACTTCTG CGGTTGGTCC TGTGTCCATG
    GCGGCTCCAA CAGCAATCAC AGAAGCAGGA ACACAGCCTC AGAAGGGTGT TTCTCAAGTC
    AAAGAAGGCC CTGTCCTAGC AACTAGTTCA GGAGCTGGTG TTTTTAAGAT GGGACGATTT
    CAGGTTTCTG TTGCAGCAGA CGGTGCCCAG AAAGAGGGTA AAAATAAGTC AGAAGATGCA
    AAGTCTGTTC ATTTTGAATC CAGCACCTCA GAGTCCTCAG TGCTATCAAG TAGTAGTCCA
    GAGAGTACCT TGGTGAAACC AGAGCCGAAT GGCATAACCA TCCCTGGTAT CTCTTCAGAT
    GTGCCAGAGA GTGCCCACAA AACTACTGCC TCAGAGGCAA AGTCAGACAC TGGGCAGCCT
    ACCAAGGTTG GACGTTTTCA GGTGACAACT ACAGCAAACA AAGTGGGTCG TTTCTCTGTA
    TCAAAAACTG AGGACAAGAT CACTGACACA AAGAAAGAAG GACCAGTGGC ATCTCCTCCT
    TTTATGGATT TGGAACAAGC TGTTCTTCCT GCTGTGATAC CAAAGAAAGA GAAGCCTGAA
    CTGTCAGAGC CTTCACATCT AAATGGGCCG TCTTCTGACC CGGAGGCCGC TTTTTTAAGT
    AGGGATGTGG ATGATGGTTC CGGTAGTCCA CACTCGCCCC ATCAGCTGAG CTCAAAGAGC
    CTTCCTAGCC AGAATCTAAG TCAAAGCCTT AGTAATTCAT TTAACTCCTC TTACATGAGT
    AGCGACAATG AGTCAGATAT CGAAGATGAA GACTTAAAGT TAGAGCTGCG ACGACTACGA
    GATAAACATC TCAAAGAGAT TCAGGACCTG CAGAGTCGCC AGAAGCATGA AATTGAATCT
    TTGTATACCA AACTGGGCAA GGTGCCCCCT GCTGTTATTA TTCCCCCAGC TGCTCCCCTT
    TCAGGGAGAA GACGACGACC CACTAAAAGC AAAGGCAGCA AATCTAGTCG AAGCAGTTCC
    TTGGGGAATA AAAGCCCCCA GCTTTCAGGT AACCTGTCTG GTCAGAGTGC AGCTTCAGTC
    TTGCACCCCC AGCAGACCCT CCACCCTCCT GGCAACATCC CAGAGTCCGG GCAGAATCAG
    CTGTTACAGC CCCTTAAGCC ATCTCCCTCC AGTGACAACC TCTATTCAGC CTTCACCAGT
    GATGGTGCCA TTTCAGTACC AAGCCTTTCT GCTCCAGGTC AAGGAACCAG CAGCACAAAC
    ACTGTTGGGG CAACAGTGAA CAGCCAAGCC GCCCAAGCTC AGCCTCCTGC CATGACGTCC
    AGCAGGAAGG GCACATTCAC AGATGACTTG CACAAGTTGG TAGACAATTG GGCCCGAGAT
    GCCATGAATC TCTCAGGCAG GAGAGGAAGC AAAGGGCACA TGAATTACGA GGGCCCTGGA
    ATGGCAAGGA AGTTCTCTGC ACCTGGGCAA CTGTGCATCT CCATGACCTC GAACCTGGGT
    GGCTCTGCCC CCATCTCTGC AGCATCAGCT ACCTCTCTAG GTCACTTCAC CAAGTCTATG
    TGCCCCCCAC AGCAGTATGG CTTTCCAGCT ACCCCATTTG GCGCTCAATG GAGTGGGACG
    GGTGGCCCAG CACCACAGCC ACTTGGCCAG TTCCAACCTG TGGGAACTGC CTCCTTGCAG
    AATTTCAACA TCAGCAATTT GCAGAAATCC ATCAGCAACC CCCCAGGCTC CAACCTGCGG
    ACCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28145
    Clone ID Related Accession (Same CDS sequence) NM_014823.2 , NM_014823.3
    Accession Version NM_014823.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6405bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase WNK1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004765.2, AJ296290.1, CD629562.1, AB002342.2, AC004803.11, BX644268.1, DA720959.1, BC141881.1 and AI074334.1. On May 27, 2010 this sequence version replaced NM_014823.1. Transcript Variant: This variant (2) uses two alternative splice sites and lacks two exons in the coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB002342.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT
    CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AACCCATACA ACATCCTCAG
    CAGCAGGGAA TACAGCAGAC AGCCCCTCCT CAACAGACAG TGCAGTATTC ACTTTCACAG
    ACATCAACCT CCAGTGAGGC CACTACTGCA CAGCCAGTGA GTCAGCCTCA AGCTCCACAA
    GTCTTGCCTC AAGTATCAGC TGGAAAACAG AGTACTCAGG GAGTCTCTCA GGTTGCTCCT
    GCAGAGCCAG TTGCAGTAGC ACAGACCCAA GCTACCCAGC CGACCACTTT GGCTTCCTCT
    GTAGACAGTG CACATTCAGA TGTTGCTTCA GGTATGAGTG ATGGCAATGA GAACGTCCCA
    TCTTCCAGTG GAAGGCATGA AGGAAGAACT ACAAAACGGC ATTACCGAAA ATCTGTAAGG
    AGTCGCTCTC GACATGAAAA AACTTCACGC CCAAAATTAA GAATTTTGAA TGTTTCAAAT
    AAAGGAGACC GAGTAGTAGA ATGTCAATTA GAGACTCATA ATAGGAAAAT GGTTACATTC
    AAATTTGACC TAGATGGTGA CAACCCCGAG GAGATAGCAA CAATTATGGT GAACAATGAC
    TTTATTCTAG CAATAGAGAG AGAGTCGTTT GTGGATCAAG TGCGAGAAAT TATTGAAAAA
    GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGATCA GGGATTGGAG
    AGTCTACAAG GAAAGGATGA CTATGGCTTT TCAGGTTCTC AGAAATTGGA AGGAGAGTTC
    AAACAACCAA TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA
    ACTCAAGTTG TTCATTCTGC GGGAAGGCGG TTTATAGTGA GTCCTGTGCC AGAAAGCCGA
    TTACGAGAAT CAAAAGTTTT CCCCAGTGAA ATAACAGATA CAGTTGCTGC CTCTACAGCT
    CAGAGCCCTG GAATGAACTT GTCTCACTCT GCATCATCCC TTAGTCTACA ACAGGCCTTT
    TCTGAACTTA GACGTGCCCA AATGACAGAA GGACCCAACA CAGCACCTCC AAACTTTAGT
    CATACAGGAC CAACATTTCC AGTAGTACCT CCTTTCTTAA GTAGCATTGC TGGAGTCCCA
    ACCACAGCAG CAGCCACAGC ACCAGTCCCT GCAACAAGCA GCCCTCCTAA TGACATTTCC
    ACATCAGTAA TTCAGTCTGA GGTTACAGTG CCCACTGAAG AGGGGATTGC TGGAGTTGCC
    ACCAGCACAG GTGTGGTAAC TTCAGGTGGT CTCCCCATAC CACCTGTGTC TGAATCACCA
    GTACTTTCCA GCGTAGTTTC AAGTATCACA ATACCTGCAG TTGTCTCAAT ATCTACTACA
    TCCCCGTCAC TTCAAGTCCC CACATCCACA TCTGAGATCG TTGTTTCTAG TACAGCACTG
    TATCCTTCAG TAACAGTTTC AGCAACTTCA GCCTCTGCAG GGGGCAGTAC TGCTACCCCA
    GGTCCTAAGC CTCCAGCTGT AGTATCTCAG CAGGCAGCAG GCAGCACTAC TGTGGGAGCC
    ACATTAACAT CAGTTTCTAC CACCACTTCA TTCCCAAGCA CAGCTTCACA GCTGTGCATT
    CAGCTTAGCA GCAGTACTTC TACTCCTACT TTAGCTGAAA CCGTGGTAGT TAGCGCACAC
    TCACTAGATA AGACATCTCA TAGCAGTACA ACTGGATTGG CTTTCTCCCT CTCTGCACCA
    TCTTCCTCTT CCTCTCCTGG AGCAGGAGTG TCTAGTTATA TTTCTCAGCC TGGTGGGCTG
    CATCCTTTGG TCATTCCATC AGTGATAGCT TCTACTCCTA TTCTTCCCCA AGCAGCAGGA
    CCTACTTCTA CACCTTTATT ACCCCAAGTA CCTAGTATCC CACCCTTGGT ACAGCCTGTT
    GCCAATGTGC CTGCTGTACA GCAGACACTA ATTCATAGTC AGCCTCAACC AGCTTTGCTT
    CCCAACCAGC CCCATACTCA TTGTCCTGAA GTAGATTCTG ATACACAACC CAAAGCTCCT
    GGAATTGATG ACATAAAGAC TCTAGAAGAA AAGCTGCGGT CTCTGTTCAG TGAACACAGC
    TCATCTGGAG CTCAGCATGC CTCTGTCTCA CTGGAGACCT CACTAGTCAT AGAGAGCACT
    GTCACACCAG GCATCCCAAC TACTGCTGTT GCACCAAGCA AACTCCTGAC TTCTACCACA
    AGTACTTGCT TACCACCAAC CAATTTACCA CTAGGAACAG TTGCTTTGCC AGTTACACCA
    GTGGTCACAC CTGGGCAAGT TTCTACCCCA GTCAGCACTA CTACATCAGG AGTGAAACCT
    GGAACTGCTC CCTCCAAGCC ACCTCTAACT AAGGCTCCGG TGCTGCCAGT GGGTACTGAA
    CTTCCAGCAG GTACTCTACC CAGCGAGCAG CTGCCACCTT TTCCAGGACC TTCTCTAACC
    CAGTCCCAGC AACCTCTAGA GGATCTTGAT GCTCAATTGA GAAGAACACT TAGTCCAGAG
    ATGATCACAG TGACTTCTGC GGTTGGTCCT GTGTCCATGG CGGCTCCAAC AGCAATCACA
    GAAGCAGGAA CACAGCCTCA GAAGGTTTCT CAAGTCAAAG AAGGCCCTGT CCTAGCAACT
    AGTTCAGGAG CTGGTGTTTT TAAGATGGGA CGATTTCAGG TTTCTGTTGC AGCAGACGGT
    GCCCAGAAAG AGGGTAAAAA TAAGTCAGAA GATGCAAAGT CTGTTCATTT TGAATCCAGC
    ACCTCAGAGT CCTCAGTGCT ATCAAGTAGT AGTCCAGAGA GTACCTTGGT GAAACCAGAG
    CCGAATGGCA TAACCATCCC TGGTATCTCT TCAGATGTGC CAGAGAGTGC CCACAAAACT
    ACTGCCTCAG AGGCAAAGTC AGACACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG
    ACAACTACAG CAAACAAAGT GGGTCGTTTC TCTGTATCAA AAACTGAGGA CAAGATCACT
    GACACAAAGA AAGAAGGACC AGTGGCATCT CCTCCTTTTA TGGATTTGGA ACAAGCTGTT
    CTTCCTGCTG TGATACCAAA GAAAGAGAAG CCTGAACTGT CAGAGCCTTC ACATCTAAAT
    GGGCCGTCTT CTGACCCGGA GGCCGCTTTT TTAAGTAGGG ATGTGGATGA TGGTTCCGGT
    AGTCCACACT CGCCCCATCA GCTGAGCTCA AAGAGCCTTC CTAGCCAGAA TCTAAGTCAA
    AGCCTTAGTA ATTCATTTAA CTCCTCTTAC ATGAGTAGCG ACAATGAGTC AGATATCGAA
    GATGAAGACT TAAAGTTAGA GCTGCGACGA CTACGAGATA AACATCTCAA AGAGATTCAG
    GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGCAAGGTG
    CCCCCTGCTG TTATTATTCC CCCAGCTGCT CCCCTTTCAG GGAGAAGACG ACGACCCACT
    AAAAGCAAAG GCAGCAAATC TAGTCGAAGC AGTTCCTTGG GGAATAAAAG CCCCCAGCTT
    TCAGGTAACC TGTCTGGTCA GAGTGCAGCT TCAGTCTTGC ACCCCCAGCA GACCCTCCAC
    CCTCCTGGCA ACATCCCAGA GTCCGGGCAG AATCAGCTGT TACAGCCCCT TAAGCCATCT
    CCCTCCAGTG ACAACCTCTA TTCAGCCTTC ACCAGTGATG GTGCCATTTC AGTACCAAGC
    CTTTCTGCTC CAGGTCAAGG AACCAGCAGC ACAAACACTG TTGGGGCAAC AGTGAACAGC
    CAAGCCGCCC AAGCTCAGCC TCCTGCCATG ACGTCCAGCA GGAAGGGCAC ATTCACAGAT
    GACTTGCACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGGAGA
    GGAAGCAAAG GGCACATGAA TTACGAGGGC CCTGGAATGG CAAGGAAGTT CTCTGCACCT
    GGGCAACTGT GCATCTCCAT GACCTCGAAC CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA
    TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTATGGCTTT
    CCAGCTACCC CATTTGGCGC TCAATGGAGT GGGACGGGTG GCCCAGCACC ACAGCCACTT
    GGCCAGTTCC AACCTGTGGG AACTGCCTCC TTGCAGAATT TCAACATCAG CAATTTGCAG
    AAATCCATCA GCAACCCCCC AGGCTCCAAC CTGCGGACCA CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_055638.2
    CDS644..7048
    Translation

    Target ORF information:

    RefSeq Version NM_014823.2
    Organism Homo sapiens(human)
    Definition Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_014823.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT
    CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AACCCATACA ACATCCTCAG
    CAGCAGGGAA TACAGCAGAC AGCCCCTCCT CAACAGACAG TGCAGTATTC ACTTTCACAG
    ACATCAACCT CCAGTGAGGC CACTACTGCA CAGCCAGTGA GTCAGCCTCA AGCTCCACAA
    GTCTTGCCTC AAGTATCAGC TGGAAAACAG AGTACTCAGG GAGTCTCTCA GGTTGCTCCT
    GCAGAGCCAG TTGCAGTAGC ACAGACCCAA GCTACCCAGC CGACCACTTT GGCTTCCTCT
    GTAGACAGTG CACATTCAGA TGTTGCTTCA GGTATGAGTG ATGGCAATGA GAACGTCCCA
    TCTTCCAGTG GAAGGCATGA AGGAAGAACT ACAAAACGGC ATTACCGAAA ATCTGTAAGG
    AGTCGCTCTC GACATGAAAA AACTTCACGC CCAAAATTAA GAATTTTGAA TGTTTCAAAT
    AAAGGAGACC GAGTAGTAGA ATGTCAATTA GAGACTCATA ATAGGAAAAT GGTTACATTC
    AAATTTGACC TAGATGGTGA CAACCCCGAG GAGATAGCAA CAATTATGGT GAACAATGAC
    TTTATTCTAG CAATAGAGAG AGAGTCGTTT GTGGATCAAG TGCGAGAAAT TATTGAAAAA
    GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGATCA GGGATTGGAG
    AGTCTACAAG GAAAGGATGA CTATGGCTTT TCAGGTTCTC AGAAATTGGA AGGAGAGTTC
    AAACAACCAA TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA
    ACTCAAGTTG TTCATTCTGC GGGAAGGCGG TTTATAGTGA GTCCTGTGCC AGAAAGCCGA
    TTACGAGAAT CAAAAGTTTT CCCCAGTGAA ATAACAGATA CAGTTGCTGC CTCTACAGCT
    CAGAGCCCTG GAATGAACTT GTCTCACTCT GCATCATCCC TTAGTCTACA ACAGGCCTTT
    TCTGAACTTA GACGTGCCCA AATGACAGAA GGACCCAACA CAGCACCTCC AAACTTTAGT
    CATACAGGAC CAACATTTCC AGTAGTACCT CCTTTCTTAA GTAGCATTGC TGGAGTCCCA
    ACCACAGCAG CAGCCACAGC ACCAGTCCCT GCAACAAGCA GCCCTCCTAA TGACATTTCC
    ACATCAGTAA TTCAGTCTGA GGTTACAGTG CCCACTGAAG AGGGGATTGC TGGAGTTGCC
    ACCAGCACAG GTGTGGTAAC TTCAGGTGGT CTCCCCATAC CACCTGTGTC TGAATCACCA
    GTACTTTCCA GCGTAGTTTC AAGTATCACA ATACCTGCAG TTGTCTCAAT ATCTACTACA
    TCCCCGTCAC TTCAAGTCCC CACATCCACA TCTGAGATCG TTGTTTCTAG TACAGCACTG
    TATCCTTCAG TAACAGTTTC AGCAACTTCA GCCTCTGCAG GGGGCAGTAC TGCTACCCCA
    GGTCCTAAGC CTCCAGCTGT AGTATCTCAG CAGGCAGCAG GCAGCACTAC TGTGGGAGCC
    ACATTAACAT CAGTTTCTAC CACCACTTCA TTCCCAAGCA CAGCTTCACA GCTGTGCATT
    CAGCTTAGCA GCAGTACTTC TACTCCTACT TTAGCTGAAA CCGTGGTAGT TAGCGCACAC
    TCACTAGATA AGACATCTCA TAGCAGTACA ACTGGATTGG CTTTCTCCCT CTCTGCACCA
    TCTTCCTCTT CCTCTCCTGG AGCAGGAGTG TCTAGTTATA TTTCTCAGCC TGGTGGGCTG
    CATCCTTTGG TCATTCCATC AGTGATAGCT TCTACTCCTA TTCTTCCCCA AGCAGCAGGA
    CCTACTTCTA CACCTTTATT ACCCCAAGTA CCTAGTATCC CACCCTTGGT ACAGCCTGTT
    GCCAATGTGC CTGCTGTACA GCAGACACTA ATTCATAGTC AGCCTCAACC AGCTTTGCTT
    CCCAACCAGC CCCATACTCA TTGTCCTGAA GTAGATTCTG ATACACAACC CAAAGCTCCT
    GGAATTGATG ACATAAAGAC TCTAGAAGAA AAGCTGCGGT CTCTGTTCAG TGAACACAGC
    TCATCTGGAG CTCAGCATGC CTCTGTCTCA CTGGAGACCT CACTAGTCAT AGAGAGCACT
    GTCACACCAG GCATCCCAAC TACTGCTGTT GCACCAAGCA AACTCCTGAC TTCTACCACA
    AGTACTTGCT TACCACCAAC CAATTTACCA CTAGGAACAG TTGCTTTGCC AGTTACACCA
    GTGGTCACAC CTGGGCAAGT TTCTACCCCA GTCAGCACTA CTACATCAGG AGTGAAACCT
    GGAACTGCTC CCTCCAAGCC ACCTCTAACT AAGGCTCCGG TGCTGCCAGT GGGTACTGAA
    CTTCCAGCAG GTACTCTACC CAGCGAGCAG CTGCCACCTT TTCCAGGACC TTCTCTAACC
    CAGTCCCAGC AACCTCTAGA GGATCTTGAT GCTCAATTGA GAAGAACACT TAGTCCAGAG
    ATGATCACAG TGACTTCTGC GGTTGGTCCT GTGTCCATGG CGGCTCCAAC AGCAATCACA
    GAAGCAGGAA CACAGCCTCA GAAGGTTTCT CAAGTCAAAG AAGGCCCTGT CCTAGCAACT
    AGTTCAGGAG CTGGTGTTTT TAAGATGGGA CGATTTCAGG TTTCTGTTGC AGCAGACGGT
    GCCCAGAAAG AGGGTAAAAA TAAGTCAGAA GATGCAAAGT CTGTTCATTT TGAATCCAGC
    ACCTCAGAGT CCTCAGTGCT ATCAAGTAGT AGTCCAGAGA GTACCTTGGT GAAACCAGAG
    CCGAATGGCA TAACCATCCC TGGTATCTCT TCAGATGTGC CAGAGAGTGC CCACAAAACT
    ACTGCCTCAG AGGCAAAGTC AGACACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG
    ACAACTACAG CAAACAAAGT GGGTCGTTTC TCTGTATCAA AAACTGAGGA CAAGATCACT
    GACACAAAGA AAGAAGGACC AGTGGCATCT CCTCCTTTTA TGGATTTGGA ACAAGCTGTT
    CTTCCTGCTG TGATACCAAA GAAAGAGAAG CCTGAACTGT CAGAGCCTTC ACATCTAAAT
    GGGCCGTCTT CTGACCCGGA GGCCGCTTTT TTAAGTAGGG ATGTGGATGA TGGTTCCGGT
    AGTCCACACT CGCCCCATCA GCTGAGCTCA AAGAGCCTTC CTAGCCAGAA TCTAAGTCAA
    AGCCTTAGTA ATTCATTTAA CTCCTCTTAC ATGAGTAGCG ACAATGAGTC AGATATCGAA
    GATGAAGACT TAAAGTTAGA GCTGCGACGA CTACGAGATA AACATCTCAA AGAGATTCAG
    GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGCAAGGTG
    CCCCCTGCTG TTATTATTCC CCCAGCTGCT CCCCTTTCAG GGAGAAGACG ACGACCCACT
    AAAAGCAAAG GCAGCAAATC TAGTCGAAGC AGTTCCTTGG GGAATAAAAG CCCCCAGCTT
    TCAGGTAACC TGTCTGGTCA GAGTGCAGCT TCAGTCTTGC ACCCCCAGCA GACCCTCCAC
    CCTCCTGGCA ACATCCCAGA GTCCGGGCAG AATCAGCTGT TACAGCCCCT TAAGCCATCT
    CCCTCCAGTG ACAACCTCTA TTCAGCCTTC ACCAGTGATG GTGCCATTTC AGTACCAAGC
    CTTTCTGCTC CAGGTCAAGG AACCAGCAGC ACAAACACTG TTGGGGCAAC AGTGAACAGC
    CAAGCCGCCC AAGCTCAGCC TCCTGCCATG ACGTCCAGCA GGAAGGGCAC ATTCACAGAT
    GACTTGCACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGGAGA
    GGAAGCAAAG GGCACATGAA TTACGAGGGC CCTGGAATGG CAAGGAAGTT CTCTGCACCT
    GGGCAACTGT GCATCTCCAT GACCTCGAAC CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA
    TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTATGGCTTT
    CCAGCTACCC CATTTGGCGC TCAATGGAGT GGGACGGGTG GCCCAGCACC ACAGCCACTT
    GGCCAGTTCC AACCTGTGGG AACTGCCTCC TTGCAGAATT TCAACATCAG CAATTTGCAG
    AAATCCATCA GCAACCCCCC AGGCTCCAAC CTGCGGACCA CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28145
    Clone ID Related Accession (Same CDS sequence) NM_014823.2 , NM_014823.3
    Accession Version NM_014823.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6405bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-27
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase WNK1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004765.2, AJ296290.1, CD629562.1, AB002342.2, AC004803.11, BX644268.1, DA720959.1, BC141881.1 and AI074334.1. On May 31, 2019 this sequence version replaced NM_014823.2. Transcript Variant: This variant (2) uses two alternative splice sites and lacks two exons in the coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB002342.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT
    CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AACCCATACA ACATCCTCAG
    CAGCAGGGAA TACAGCAGAC AGCCCCTCCT CAACAGACAG TGCAGTATTC ACTTTCACAG
    ACATCAACCT CCAGTGAGGC CACTACTGCA CAGCCAGTGA GTCAGCCTCA AGCTCCACAA
    GTCTTGCCTC AAGTATCAGC TGGAAAACAG AGTACTCAGG GAGTCTCTCA GGTTGCTCCT
    GCAGAGCCAG TTGCAGTAGC ACAGACCCAA GCTACCCAGC CGACCACTTT GGCTTCCTCT
    GTAGACAGTG CACATTCAGA TGTTGCTTCA GGTATGAGTG ATGGCAATGA GAACGTCCCA
    TCTTCCAGTG GAAGGCATGA AGGAAGAACT ACAAAACGGC ATTACCGAAA ATCTGTAAGG
    AGTCGCTCTC GACATGAAAA AACTTCACGC CCAAAATTAA GAATTTTGAA TGTTTCAAAT
    AAAGGAGACC GAGTAGTAGA ATGTCAATTA GAGACTCATA ATAGGAAAAT GGTTACATTC
    AAATTTGACC TAGATGGTGA CAACCCCGAG GAGATAGCAA CAATTATGGT GAACAATGAC
    TTTATTCTAG CAATAGAGAG AGAGTCGTTT GTGGATCAAG TGCGAGAAAT TATTGAAAAA
    GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGATCA GGGATTGGAG
    AGTCTACAAG GAAAGGATGA CTATGGCTTT TCAGGTTCTC AGAAATTGGA AGGAGAGTTC
    AAACAACCAA TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA
    ACTCAAGTTG TTCATTCTGC GGGAAGGCGG TTTATAGTGA GTCCTGTGCC AGAAAGCCGA
    TTACGAGAAT CAAAAGTTTT CCCCAGTGAA ATAACAGATA CAGTTGCTGC CTCTACAGCT
    CAGAGCCCTG GAATGAACTT GTCTCACTCT GCATCATCCC TTAGTCTACA ACAGGCCTTT
    TCTGAACTTA GACGTGCCCA AATGACAGAA GGACCCAACA CAGCACCTCC AAACTTTAGT
    CATACAGGAC CAACATTTCC AGTAGTACCT CCTTTCTTAA GTAGCATTGC TGGAGTCCCA
    ACCACAGCAG CAGCCACAGC ACCAGTCCCT GCAACAAGCA GCCCTCCTAA TGACATTTCC
    ACATCAGTAA TTCAGTCTGA GGTTACAGTG CCCACTGAAG AGGGGATTGC TGGAGTTGCC
    ACCAGCACAG GTGTGGTAAC TTCAGGTGGT CTCCCCATAC CACCTGTGTC TGAATCACCA
    GTACTTTCCA GCGTAGTTTC AAGTATCACA ATACCTGCAG TTGTCTCAAT ATCTACTACA
    TCCCCGTCAC TTCAAGTCCC CACATCCACA TCTGAGATCG TTGTTTCTAG TACAGCACTG
    TATCCTTCAG TAACAGTTTC AGCAACTTCA GCCTCTGCAG GGGGCAGTAC TGCTACCCCA
    GGTCCTAAGC CTCCAGCTGT AGTATCTCAG CAGGCAGCAG GCAGCACTAC TGTGGGAGCC
    ACATTAACAT CAGTTTCTAC CACCACTTCA TTCCCAAGCA CAGCTTCACA GCTGTGCATT
    CAGCTTAGCA GCAGTACTTC TACTCCTACT TTAGCTGAAA CCGTGGTAGT TAGCGCACAC
    TCACTAGATA AGACATCTCA TAGCAGTACA ACTGGATTGG CTTTCTCCCT CTCTGCACCA
    TCTTCCTCTT CCTCTCCTGG AGCAGGAGTG TCTAGTTATA TTTCTCAGCC TGGTGGGCTG
    CATCCTTTGG TCATTCCATC AGTGATAGCT TCTACTCCTA TTCTTCCCCA AGCAGCAGGA
    CCTACTTCTA CACCTTTATT ACCCCAAGTA CCTAGTATCC CACCCTTGGT ACAGCCTGTT
    GCCAATGTGC CTGCTGTACA GCAGACACTA ATTCATAGTC AGCCTCAACC AGCTTTGCTT
    CCCAACCAGC CCCATACTCA TTGTCCTGAA GTAGATTCTG ATACACAACC CAAAGCTCCT
    GGAATTGATG ACATAAAGAC TCTAGAAGAA AAGCTGCGGT CTCTGTTCAG TGAACACAGC
    TCATCTGGAG CTCAGCATGC CTCTGTCTCA CTGGAGACCT CACTAGTCAT AGAGAGCACT
    GTCACACCAG GCATCCCAAC TACTGCTGTT GCACCAAGCA AACTCCTGAC TTCTACCACA
    AGTACTTGCT TACCACCAAC CAATTTACCA CTAGGAACAG TTGCTTTGCC AGTTACACCA
    GTGGTCACAC CTGGGCAAGT TTCTACCCCA GTCAGCACTA CTACATCAGG AGTGAAACCT
    GGAACTGCTC CCTCCAAGCC ACCTCTAACT AAGGCTCCGG TGCTGCCAGT GGGTACTGAA
    CTTCCAGCAG GTACTCTACC CAGCGAGCAG CTGCCACCTT TTCCAGGACC TTCTCTAACC
    CAGTCCCAGC AACCTCTAGA GGATCTTGAT GCTCAATTGA GAAGAACACT TAGTCCAGAG
    ATGATCACAG TGACTTCTGC GGTTGGTCCT GTGTCCATGG CGGCTCCAAC AGCAATCACA
    GAAGCAGGAA CACAGCCTCA GAAGGTTTCT CAAGTCAAAG AAGGCCCTGT CCTAGCAACT
    AGTTCAGGAG CTGGTGTTTT TAAGATGGGA CGATTTCAGG TTTCTGTTGC AGCAGACGGT
    GCCCAGAAAG AGGGTAAAAA TAAGTCAGAA GATGCAAAGT CTGTTCATTT TGAATCCAGC
    ACCTCAGAGT CCTCAGTGCT ATCAAGTAGT AGTCCAGAGA GTACCTTGGT GAAACCAGAG
    CCGAATGGCA TAACCATCCC TGGTATCTCT TCAGATGTGC CAGAGAGTGC CCACAAAACT
    ACTGCCTCAG AGGCAAAGTC AGACACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG
    ACAACTACAG CAAACAAAGT GGGTCGTTTC TCTGTATCAA AAACTGAGGA CAAGATCACT
    GACACAAAGA AAGAAGGACC AGTGGCATCT CCTCCTTTTA TGGATTTGGA ACAAGCTGTT
    CTTCCTGCTG TGATACCAAA GAAAGAGAAG CCTGAACTGT CAGAGCCTTC ACATCTAAAT
    GGGCCGTCTT CTGACCCGGA GGCCGCTTTT TTAAGTAGGG ATGTGGATGA TGGTTCCGGT
    AGTCCACACT CGCCCCATCA GCTGAGCTCA AAGAGCCTTC CTAGCCAGAA TCTAAGTCAA
    AGCCTTAGTA ATTCATTTAA CTCCTCTTAC ATGAGTAGCG ACAATGAGTC AGATATCGAA
    GATGAAGACT TAAAGTTAGA GCTGCGACGA CTACGAGATA AACATCTCAA AGAGATTCAG
    GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGCAAGGTG
    CCCCCTGCTG TTATTATTCC CCCAGCTGCT CCCCTTTCAG GGAGAAGACG ACGACCCACT
    AAAAGCAAAG GCAGCAAATC TAGTCGAAGC AGTTCCTTGG GGAATAAAAG CCCCCAGCTT
    TCAGGTAACC TGTCTGGTCA GAGTGCAGCT TCAGTCTTGC ACCCCCAGCA GACCCTCCAC
    CCTCCTGGCA ACATCCCAGA GTCCGGGCAG AATCAGCTGT TACAGCCCCT TAAGCCATCT
    CCCTCCAGTG ACAACCTCTA TTCAGCCTTC ACCAGTGATG GTGCCATTTC AGTACCAAGC
    CTTTCTGCTC CAGGTCAAGG AACCAGCAGC ACAAACACTG TTGGGGCAAC AGTGAACAGC
    CAAGCCGCCC AAGCTCAGCC TCCTGCCATG ACGTCCAGCA GGAAGGGCAC ATTCACAGAT
    GACTTGCACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGGAGA
    GGAAGCAAAG GGCACATGAA TTACGAGGGC CCTGGAATGG CAAGGAAGTT CTCTGCACCT
    GGGCAACTGT GCATCTCCAT GACCTCGAAC CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA
    TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTATGGCTTT
    CCAGCTACCC CATTTGGCGC TCAATGGAGT GGGACGGGTG GCCCAGCACC ACAGCCACTT
    GGCCAGTTCC AACCTGTGGG AACTGCCTCC TTGCAGAATT TCAACATCAG CAATTTGCAG
    AAATCCATCA GCAACCCCCC AGGCTCCAAC CTGCGGACCA CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_055638.2
    CDS988..7392
    Translation

    Target ORF information:

    RefSeq Version NM_014823.3
    Organism Homo sapiens(human)
    Definition Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_014823.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT
    CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AACCCATACA ACATCCTCAG
    CAGCAGGGAA TACAGCAGAC AGCCCCTCCT CAACAGACAG TGCAGTATTC ACTTTCACAG
    ACATCAACCT CCAGTGAGGC CACTACTGCA CAGCCAGTGA GTCAGCCTCA AGCTCCACAA
    GTCTTGCCTC AAGTATCAGC TGGAAAACAG AGTACTCAGG GAGTCTCTCA GGTTGCTCCT
    GCAGAGCCAG TTGCAGTAGC ACAGACCCAA GCTACCCAGC CGACCACTTT GGCTTCCTCT
    GTAGACAGTG CACATTCAGA TGTTGCTTCA GGTATGAGTG ATGGCAATGA GAACGTCCCA
    TCTTCCAGTG GAAGGCATGA AGGAAGAACT ACAAAACGGC ATTACCGAAA ATCTGTAAGG
    AGTCGCTCTC GACATGAAAA AACTTCACGC CCAAAATTAA GAATTTTGAA TGTTTCAAAT
    AAAGGAGACC GAGTAGTAGA ATGTCAATTA GAGACTCATA ATAGGAAAAT GGTTACATTC
    AAATTTGACC TAGATGGTGA CAACCCCGAG GAGATAGCAA CAATTATGGT GAACAATGAC
    TTTATTCTAG CAATAGAGAG AGAGTCGTTT GTGGATCAAG TGCGAGAAAT TATTGAAAAA
    GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGATCA GGGATTGGAG
    AGTCTACAAG GAAAGGATGA CTATGGCTTT TCAGGTTCTC AGAAATTGGA AGGAGAGTTC
    AAACAACCAA TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA
    ACTCAAGTTG TTCATTCTGC GGGAAGGCGG TTTATAGTGA GTCCTGTGCC AGAAAGCCGA
    TTACGAGAAT CAAAAGTTTT CCCCAGTGAA ATAACAGATA CAGTTGCTGC CTCTACAGCT
    CAGAGCCCTG GAATGAACTT GTCTCACTCT GCATCATCCC TTAGTCTACA ACAGGCCTTT
    TCTGAACTTA GACGTGCCCA AATGACAGAA GGACCCAACA CAGCACCTCC AAACTTTAGT
    CATACAGGAC CAACATTTCC AGTAGTACCT CCTTTCTTAA GTAGCATTGC TGGAGTCCCA
    ACCACAGCAG CAGCCACAGC ACCAGTCCCT GCAACAAGCA GCCCTCCTAA TGACATTTCC
    ACATCAGTAA TTCAGTCTGA GGTTACAGTG CCCACTGAAG AGGGGATTGC TGGAGTTGCC
    ACCAGCACAG GTGTGGTAAC TTCAGGTGGT CTCCCCATAC CACCTGTGTC TGAATCACCA
    GTACTTTCCA GCGTAGTTTC AAGTATCACA ATACCTGCAG TTGTCTCAAT ATCTACTACA
    TCCCCGTCAC TTCAAGTCCC CACATCCACA TCTGAGATCG TTGTTTCTAG TACAGCACTG
    TATCCTTCAG TAACAGTTTC AGCAACTTCA GCCTCTGCAG GGGGCAGTAC TGCTACCCCA
    GGTCCTAAGC CTCCAGCTGT AGTATCTCAG CAGGCAGCAG GCAGCACTAC TGTGGGAGCC
    ACATTAACAT CAGTTTCTAC CACCACTTCA TTCCCAAGCA CAGCTTCACA GCTGTGCATT
    CAGCTTAGCA GCAGTACTTC TACTCCTACT TTAGCTGAAA CCGTGGTAGT TAGCGCACAC
    TCACTAGATA AGACATCTCA TAGCAGTACA ACTGGATTGG CTTTCTCCCT CTCTGCACCA
    TCTTCCTCTT CCTCTCCTGG AGCAGGAGTG TCTAGTTATA TTTCTCAGCC TGGTGGGCTG
    CATCCTTTGG TCATTCCATC AGTGATAGCT TCTACTCCTA TTCTTCCCCA AGCAGCAGGA
    CCTACTTCTA CACCTTTATT ACCCCAAGTA CCTAGTATCC CACCCTTGGT ACAGCCTGTT
    GCCAATGTGC CTGCTGTACA GCAGACACTA ATTCATAGTC AGCCTCAACC AGCTTTGCTT
    CCCAACCAGC CCCATACTCA TTGTCCTGAA GTAGATTCTG ATACACAACC CAAAGCTCCT
    GGAATTGATG ACATAAAGAC TCTAGAAGAA AAGCTGCGGT CTCTGTTCAG TGAACACAGC
    TCATCTGGAG CTCAGCATGC CTCTGTCTCA CTGGAGACCT CACTAGTCAT AGAGAGCACT
    GTCACACCAG GCATCCCAAC TACTGCTGTT GCACCAAGCA AACTCCTGAC TTCTACCACA
    AGTACTTGCT TACCACCAAC CAATTTACCA CTAGGAACAG TTGCTTTGCC AGTTACACCA
    GTGGTCACAC CTGGGCAAGT TTCTACCCCA GTCAGCACTA CTACATCAGG AGTGAAACCT
    GGAACTGCTC CCTCCAAGCC ACCTCTAACT AAGGCTCCGG TGCTGCCAGT GGGTACTGAA
    CTTCCAGCAG GTACTCTACC CAGCGAGCAG CTGCCACCTT TTCCAGGACC TTCTCTAACC
    CAGTCCCAGC AACCTCTAGA GGATCTTGAT GCTCAATTGA GAAGAACACT TAGTCCAGAG
    ATGATCACAG TGACTTCTGC GGTTGGTCCT GTGTCCATGG CGGCTCCAAC AGCAATCACA
    GAAGCAGGAA CACAGCCTCA GAAGGTTTCT CAAGTCAAAG AAGGCCCTGT CCTAGCAACT
    AGTTCAGGAG CTGGTGTTTT TAAGATGGGA CGATTTCAGG TTTCTGTTGC AGCAGACGGT
    GCCCAGAAAG AGGGTAAAAA TAAGTCAGAA GATGCAAAGT CTGTTCATTT TGAATCCAGC
    ACCTCAGAGT CCTCAGTGCT ATCAAGTAGT AGTCCAGAGA GTACCTTGGT GAAACCAGAG
    CCGAATGGCA TAACCATCCC TGGTATCTCT TCAGATGTGC CAGAGAGTGC CCACAAAACT
    ACTGCCTCAG AGGCAAAGTC AGACACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG
    ACAACTACAG CAAACAAAGT GGGTCGTTTC TCTGTATCAA AAACTGAGGA CAAGATCACT
    GACACAAAGA AAGAAGGACC AGTGGCATCT CCTCCTTTTA TGGATTTGGA ACAAGCTGTT
    CTTCCTGCTG TGATACCAAA GAAAGAGAAG CCTGAACTGT CAGAGCCTTC ACATCTAAAT
    GGGCCGTCTT CTGACCCGGA GGCCGCTTTT TTAAGTAGGG ATGTGGATGA TGGTTCCGGT
    AGTCCACACT CGCCCCATCA GCTGAGCTCA AAGAGCCTTC CTAGCCAGAA TCTAAGTCAA
    AGCCTTAGTA ATTCATTTAA CTCCTCTTAC ATGAGTAGCG ACAATGAGTC AGATATCGAA
    GATGAAGACT TAAAGTTAGA GCTGCGACGA CTACGAGATA AACATCTCAA AGAGATTCAG
    GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGCAAGGTG
    CCCCCTGCTG TTATTATTCC CCCAGCTGCT CCCCTTTCAG GGAGAAGACG ACGACCCACT
    AAAAGCAAAG GCAGCAAATC TAGTCGAAGC AGTTCCTTGG GGAATAAAAG CCCCCAGCTT
    TCAGGTAACC TGTCTGGTCA GAGTGCAGCT TCAGTCTTGC ACCCCCAGCA GACCCTCCAC
    CCTCCTGGCA ACATCCCAGA GTCCGGGCAG AATCAGCTGT TACAGCCCCT TAAGCCATCT
    CCCTCCAGTG ACAACCTCTA TTCAGCCTTC ACCAGTGATG GTGCCATTTC AGTACCAAGC
    CTTTCTGCTC CAGGTCAAGG AACCAGCAGC ACAAACACTG TTGGGGCAAC AGTGAACAGC
    CAAGCCGCCC AAGCTCAGCC TCCTGCCATG ACGTCCAGCA GGAAGGGCAC ATTCACAGAT
    GACTTGCACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGGAGA
    GGAAGCAAAG GGCACATGAA TTACGAGGGC CCTGGAATGG CAAGGAAGTT CTCTGCACCT
    GGGCAACTGT GCATCTCCAT GACCTCGAAC CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA
    TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTATGGCTTT
    CCAGCTACCC CATTTGGCGC TCAATGGAGT GGGACGGGTG GCCCAGCACC ACAGCCACTT
    GGCCAGTTCC AACCTGTGGG AACTGCCTCC TTGCAGAATT TCAACATCAG CAATTTGCAG
    AAATCCATCA GCAACCCCCC AGGCTCCAAC CTGCGGACCA CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28188
    Clone ID Related Accession (Same CDS sequence) NM_018979.3 , NM_018979.4
    Accession Version NM_018979.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-28
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase WNK1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004765.2, AJ296290.1, CD629562.1, AB002342.2, BX644268.1, DA720959.1, AC004803.11, BC141881.1 and AI074334.1. This sequence is a reference standard in the RefSeqGene project. On May 27, 2010 this sequence version replaced NM_018979.2. Transcript Variant: This variant (1) encodes the most common isoform (1), as indicated in PubMed ID 18521183. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ296290.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT
    CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AACCCATACA ACATCCTCAG
    CAGCAGCAGG GAATACAGCA GACAGCCCCT CCTCAACAGA CAGTGCAGTA TTCACTTTCA
    CAGACATCAA CCTCCAGTGA GGCCACTACT GCACAGCCAG TGAGTCAGCC TCAAGCTCCA
    CAAGTCTTGC CTCAAGTATC AGCTGGAAAA CAGCTTCCAG TTTCCCAGCC AGTACCAACT
    ATCCAAGGCG AACCTCAGAT CCCAGTTGCG ACACAACCCT CGGTTGTTCC AGTCCACTCT
    GGTGCTCATT TCCTTCCAGT GGGACAGCCG CTCCCTACTC CCTTGCTCCC TCAGTACCCT
    GTCTCTCAGA TTCCCATATC AACTCCTCAT GTGTCTACGG CTCAGACAGG TTTCTCATCC
    CTTCCCATCA CAATGGCAGC TGGCATTACT CAGCCTCTGC TCACGTTGGC TTCATCTGCT
    ACAACAGCTG CGATCCCGGG GGTATCAACT GTGGTTCCTA GTCAGCTTCC AACCCTTCTG
    CAGCCTGTGA CTCAGCTGCC AAGTCAGGTT CACCCACAGC TCCTACAACC AGCAGTTCAG
    TCCATGGGAA TACCAGCTAA CCTTGGACAA GCTGCTGAGG TTCCACTTTC CTCTGGAGAT
    GTTCTGTACC AGGGCTTCCC ACCTCGACTG CCACCACAGT ACCCAGGAGA TTCAAATATT
    GCTCCCTCTT CCAACGTGGC TTCTGTTTGC ATCCATTCTA CAGTCCTATC CCCTCCCATG
    CCGACAGAAG TACTGGCTAC ACCTGGGTAC TTTCCCACAG TGGTGCAGCC TTATGTGGAA
    TCAAATCTTT TAGTTCCTAT GGGTGGTGTA GGAGGACAGG TTCAAGTGTC CCAGCCAGGA
    GGGAGTTTAG CACAAGCCCC CACTACATCC TCCCAGCAAG CAGTTTTGGA GAGTACTCAG
    GGAGTCTCTC AGGTTGCTCC TGCAGAGCCA GTTGCAGTAG CACAGACCCA AGCTACCCAG
    CCGACCACTT TGGCTTCCTC TGTAGACAGT GCACATTCAG ATGTTGCTTC AGGTATGAGT
    GATGGCAATG AGAACGTCCC ATCTTCCAGT GGAAGGCATG AAGGAAGAAC TACAAAACGG
    CATTACCGAA AATCTGTAAG GAGTCGCTCT CGACATGAAA AAACTTCACG CCCAAAATTA
    AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAATT AGAGACTCAT
    AATAGGAAAA TGGTTACATT CAAATTTGAC CTAGATGGTG ACAACCCCGA GGAGATAGCA
    ACAATTATGG TGAACAATGA CTTTATTCTA GCAATAGAGA GAGAGTCGTT TGTGGATCAA
    GTGCGAGAAA TTATTGAAAA AGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA
    GAGGGTGATC AGGGATTGGA GAGTCTACAA GGAAAGGATG ACTATGGCTT TTCAGGTTCT
    CAGAAATTGG AAGGAGAGTT CAAACAACCA ATTCCTGCGT CTTCCATGCC ACAGCAAATA
    GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CGGGAAGGCG GTTTATAGTG
    AGTCCTGTGC CAGAAAGCCG ATTACGAGAA TCAAAAGTTT TCCCCAGTGA AATAACAGAT
    ACAGTTGCTG CCTCTACAGC TCAGAGCCCT GGAATGAACT TGTCTCACTC TGCATCATCC
    CTTAGTCTAC AACAGGCCTT TTCTGAACTT AGACGTGCCC AAATGACAGA AGGACCCAAC
    ACAGCACCTC CAAACTTTAG TCATACAGGA CCAACATTTC CAGTAGTACC TCCTTTCTTA
    AGTAGCATTG CTGGAGTCCC AACCACAGCA GCAGCCACAG CACCAGTCCC TGCAACAAGC
    AGCCCTCCTA ATGACATTTC CACATCAGTA ATTCAGTCTG AGGTTACAGT GCCCACTGAA
    GAGGGGATTG CTGGAGTTGC CACCAGCACA GGTGTGGTAA CTTCAGGTGG TCTCCCCATA
    CCACCTGTGT CTGAATCACC AGTACTTTCC AGCGTAGTTT CAAGTATCAC AATACCTGCA
    GTTGTCTCAA TATCTACTAC ATCCCCGTCA CTTCAAGTCC CCACATCCAC ATCTGAGATC
    GTTGTTTCTA GTACAGCACT GTATCCTTCA GTAACAGTTT CAGCAACTTC AGCCTCTGCA
    GGGGGCAGTA CTGCTACCCC AGGTCCTAAG CCTCCAGCTG TAGTATCTCA GCAGGCAGCA
    GGCAGCACTA CTGTGGGAGC CACATTAACA TCAGTTTCTA CCACCACTTC ATTCCCAAGC
    ACAGCTTCAC AGCTGTGCAT TCAGCTTAGC AGCAGTACTT CTACTCCTAC TTTAGCTGAA
    ACCGTGGTAG TTAGCGCACA CTCACTAGAT AAGACATCTC ATAGCAGTAC AACTGGATTG
    GCTTTCTCCC TCTCTGCACC ATCTTCCTCT TCCTCTCCTG GAGCAGGAGT GTCTAGTTAT
    ATTTCTCAGC CTGGTGGGCT GCATCCTTTG GTCATTCCAT CAGTGATAGC TTCTACTCCT
    ATTCTTCCCC AAGCAGCAGG ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAGTATC
    CCACCCTTGG TACAGCCTGT TGCCAATGTG CCTGCTGTAC AGCAGACACT AATTCATAGT
    CAGCCTCAAC CAGCTTTGCT TCCCAACCAG CCCCATACTC ATTGTCCTGA AGTAGATTCT
    GATACACAAC CCAAAGCTCC TGGAATTGAT GACATAAAGA CTCTAGAAGA AAAGCTGCGG
    TCTCTGTTCA GTGAACACAG CTCATCTGGA GCTCAGCATG CCTCTGTCTC ACTGGAGACC
    TCACTAGTCA TAGAGAGCAC TGTCACACCA GGCATCCCAA CTACTGCTGT TGCACCAAGC
    AAACTCCTGA CTTCTACCAC AAGTACTTGC TTACCACCAA CCAATTTACC ACTAGGAACA
    GTTGCTTTGC CAGTTACACC AGTGGTCACA CCTGGGCAAG TTTCTACCCC AGTCAGCACT
    ACTACATCAG GAGTGAAACC TGGAACTGCT CCCTCCAAGC CACCTCTAAC TAAGGCTCCG
    GTGCTGCCAG TGGGTACTGA ACTTCCAGCA GGTACTCTAC CCAGCGAGCA GCTGCCACCT
    TTTCCAGGAC CTTCTCTAAC CCAGTCCCAG CAACCTCTAG AGGATCTTGA TGCTCAATTG
    AGAAGAACAC TTAGTCCAGA GATGATCACA GTGACTTCTG CGGTTGGTCC TGTGTCCATG
    GCGGCTCCAA CAGCAATCAC AGAAGCAGGA ACACAGCCTC AGAAGGGTGT TTCTCAAGTC
    AAAGAAGGCC CTGTCCTAGC AACTAGTTCA GGAGCTGGTG TTTTTAAGAT GGGACGATTT
    CAGGTTTCTG TTGCAGCAGA CGGTGCCCAG AAAGAGGGTA AAAATAAGTC AGAAGATGCA
    AAGTCTGTTC ATTTTGAATC CAGCACCTCA GAGTCCTCAG TGCTATCAAG TAGTAGTCCA
    GAGAGTACCT TGGTGAAACC AGAGCCGAAT GGCATAACCA TCCCTGGTAT CTCTTCAGAT
    GTGCCAGAGA GTGCCCACAA AACTACTGCC TCAGAGGCAA AGTCAGACAC TGGGCAGCCT
    ACCAAGGTTG GACGTTTTCA GGTGACAACT ACAGCAAACA AAGTGGGTCG TTTCTCTGTA
    TCAAAAACTG AGGACAAGAT CACTGACACA AAGAAAGAAG GACCAGTGGC ATCTCCTCCT
    TTTATGGATT TGGAACAAGC TGTTCTTCCT GCTGTGATAC CAAAGAAAGA GAAGCCTGAA
    CTGTCAGAGC CTTCACATCT AAATGGGCCG TCTTCTGACC CGGAGGCCGC TTTTTTAAGT
    AGGGATGTGG ATGATGGTTC CGGTAGTCCA CACTCGCCCC ATCAGCTGAG CTCAAAGAGC
    CTTCCTAGCC AGAATCTAAG TCAAAGCCTT AGTAATTCAT TTAACTCCTC TTACATGAGT
    AGCGACAATG AGTCAGATAT CGAAGATGAA GACTTAAAGT TAGAGCTGCG ACGACTACGA
    GATAAACATC TCAAAGAGAT TCAGGACCTG CAGAGTCGCC AGAAGCATGA AATTGAATCT
    TTGTATACCA AACTGGGCAA GGTGCCCCCT GCTGTTATTA TTCCCCCAGC TGCTCCCCTT
    TCAGGGAGAA GACGACGACC CACTAAAAGC AAAGGCAGCA AATCTAGTCG AAGCAGTTCC
    TTGGGGAATA AAAGCCCCCA GCTTTCAGGT AACCTGTCTG GTCAGAGTGC AGCTTCAGTC
    TTGCACCCCC AGCAGACCCT CCACCCTCCT GGCAACATCC CAGAGTCCGG GCAGAATCAG
    CTGTTACAGC CCCTTAAGCC ATCTCCCTCC AGTGACAACC TCTATTCAGC CTTCACCAGT
    GATGGTGCCA TTTCAGTACC AAGCCTTTCT GCTCCAGGTC AAGGAACCAG CAGCACAAAC
    ACTGTTGGGG CAACAGTGAA CAGCCAAGCC GCCCAAGCTC AGCCTCCTGC CATGACGTCC
    AGCAGGAAGG GCACATTCAC AGATGACTTG CACAAGTTGG TAGACAATTG GGCCCGAGAT
    GCCATGAATC TCTCAGGCAG GAGAGGAAGC AAAGGGCACA TGAATTACGA GGGCCCTGGA
    ATGGCAAGGA AGTTCTCTGC ACCTGGGCAA CTGTGCATCT CCATGACCTC GAACCTGGGT
    GGCTCTGCCC CCATCTCTGC AGCATCAGCT ACCTCTCTAG GTCACTTCAC CAAGTCTATG
    TGCCCCCCAC AGCAGTATGG CTTTCCAGCT ACCCCATTTG GCGCTCAATG GAGTGGGACG
    GGTGGCCCAG CACCACAGCC ACTTGGCCAG TTCCAACCTG TGGGAACTGC CTCCTTGCAG
    AATTTCAACA TCAGCAATTT GCAGAAATCC ATCAGCAACC CCCCAGGCTC CAACCTGCGG
    ACCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_061852.3
    CDS644..7792
    Translation

    Target ORF information:

    RefSeq Version NM_018979.3
    Organism Homo sapiens(human)
    Definition Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_018979.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT
    CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AACCCATACA ACATCCTCAG
    CAGCAGCAGG GAATACAGCA GACAGCCCCT CCTCAACAGA CAGTGCAGTA TTCACTTTCA
    CAGACATCAA CCTCCAGTGA GGCCACTACT GCACAGCCAG TGAGTCAGCC TCAAGCTCCA
    CAAGTCTTGC CTCAAGTATC AGCTGGAAAA CAGCTTCCAG TTTCCCAGCC AGTACCAACT
    ATCCAAGGCG AACCTCAGAT CCCAGTTGCG ACACAACCCT CGGTTGTTCC AGTCCACTCT
    GGTGCTCATT TCCTTCCAGT GGGACAGCCG CTCCCTACTC CCTTGCTCCC TCAGTACCCT
    GTCTCTCAGA TTCCCATATC AACTCCTCAT GTGTCTACGG CTCAGACAGG TTTCTCATCC
    CTTCCCATCA CAATGGCAGC TGGCATTACT CAGCCTCTGC TCACGTTGGC TTCATCTGCT
    ACAACAGCTG CGATCCCGGG GGTATCAACT GTGGTTCCTA GTCAGCTTCC AACCCTTCTG
    CAGCCTGTGA CTCAGCTGCC AAGTCAGGTT CACCCACAGC TCCTACAACC AGCAGTTCAG
    TCCATGGGAA TACCAGCTAA CCTTGGACAA GCTGCTGAGG TTCCACTTTC CTCTGGAGAT
    GTTCTGTACC AGGGCTTCCC ACCTCGACTG CCACCACAGT ACCCAGGAGA TTCAAATATT
    GCTCCCTCTT CCAACGTGGC TTCTGTTTGC ATCCATTCTA CAGTCCTATC CCCTCCCATG
    CCGACAGAAG TACTGGCTAC ACCTGGGTAC TTTCCCACAG TGGTGCAGCC TTATGTGGAA
    TCAAATCTTT TAGTTCCTAT GGGTGGTGTA GGAGGACAGG TTCAAGTGTC CCAGCCAGGA
    GGGAGTTTAG CACAAGCCCC CACTACATCC TCCCAGCAAG CAGTTTTGGA GAGTACTCAG
    GGAGTCTCTC AGGTTGCTCC TGCAGAGCCA GTTGCAGTAG CACAGACCCA AGCTACCCAG
    CCGACCACTT TGGCTTCCTC TGTAGACAGT GCACATTCAG ATGTTGCTTC AGGTATGAGT
    GATGGCAATG AGAACGTCCC ATCTTCCAGT GGAAGGCATG AAGGAAGAAC TACAAAACGG
    CATTACCGAA AATCTGTAAG GAGTCGCTCT CGACATGAAA AAACTTCACG CCCAAAATTA
    AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAATT AGAGACTCAT
    AATAGGAAAA TGGTTACATT CAAATTTGAC CTAGATGGTG ACAACCCCGA GGAGATAGCA
    ACAATTATGG TGAACAATGA CTTTATTCTA GCAATAGAGA GAGAGTCGTT TGTGGATCAA
    GTGCGAGAAA TTATTGAAAA AGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA
    GAGGGTGATC AGGGATTGGA GAGTCTACAA GGAAAGGATG ACTATGGCTT TTCAGGTTCT
    CAGAAATTGG AAGGAGAGTT CAAACAACCA ATTCCTGCGT CTTCCATGCC ACAGCAAATA
    GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CGGGAAGGCG GTTTATAGTG
    AGTCCTGTGC CAGAAAGCCG ATTACGAGAA TCAAAAGTTT TCCCCAGTGA AATAACAGAT
    ACAGTTGCTG CCTCTACAGC TCAGAGCCCT GGAATGAACT TGTCTCACTC TGCATCATCC
    CTTAGTCTAC AACAGGCCTT TTCTGAACTT AGACGTGCCC AAATGACAGA AGGACCCAAC
    ACAGCACCTC CAAACTTTAG TCATACAGGA CCAACATTTC CAGTAGTACC TCCTTTCTTA
    AGTAGCATTG CTGGAGTCCC AACCACAGCA GCAGCCACAG CACCAGTCCC TGCAACAAGC
    AGCCCTCCTA ATGACATTTC CACATCAGTA ATTCAGTCTG AGGTTACAGT GCCCACTGAA
    GAGGGGATTG CTGGAGTTGC CACCAGCACA GGTGTGGTAA CTTCAGGTGG TCTCCCCATA
    CCACCTGTGT CTGAATCACC AGTACTTTCC AGCGTAGTTT CAAGTATCAC AATACCTGCA
    GTTGTCTCAA TATCTACTAC ATCCCCGTCA CTTCAAGTCC CCACATCCAC ATCTGAGATC
    GTTGTTTCTA GTACAGCACT GTATCCTTCA GTAACAGTTT CAGCAACTTC AGCCTCTGCA
    GGGGGCAGTA CTGCTACCCC AGGTCCTAAG CCTCCAGCTG TAGTATCTCA GCAGGCAGCA
    GGCAGCACTA CTGTGGGAGC CACATTAACA TCAGTTTCTA CCACCACTTC ATTCCCAAGC
    ACAGCTTCAC AGCTGTGCAT TCAGCTTAGC AGCAGTACTT CTACTCCTAC TTTAGCTGAA
    ACCGTGGTAG TTAGCGCACA CTCACTAGAT AAGACATCTC ATAGCAGTAC AACTGGATTG
    GCTTTCTCCC TCTCTGCACC ATCTTCCTCT TCCTCTCCTG GAGCAGGAGT GTCTAGTTAT
    ATTTCTCAGC CTGGTGGGCT GCATCCTTTG GTCATTCCAT CAGTGATAGC TTCTACTCCT
    ATTCTTCCCC AAGCAGCAGG ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAGTATC
    CCACCCTTGG TACAGCCTGT TGCCAATGTG CCTGCTGTAC AGCAGACACT AATTCATAGT
    CAGCCTCAAC CAGCTTTGCT TCCCAACCAG CCCCATACTC ATTGTCCTGA AGTAGATTCT
    GATACACAAC CCAAAGCTCC TGGAATTGAT GACATAAAGA CTCTAGAAGA AAAGCTGCGG
    TCTCTGTTCA GTGAACACAG CTCATCTGGA GCTCAGCATG CCTCTGTCTC ACTGGAGACC
    TCACTAGTCA TAGAGAGCAC TGTCACACCA GGCATCCCAA CTACTGCTGT TGCACCAAGC
    AAACTCCTGA CTTCTACCAC AAGTACTTGC TTACCACCAA CCAATTTACC ACTAGGAACA
    GTTGCTTTGC CAGTTACACC AGTGGTCACA CCTGGGCAAG TTTCTACCCC AGTCAGCACT
    ACTACATCAG GAGTGAAACC TGGAACTGCT CCCTCCAAGC CACCTCTAAC TAAGGCTCCG
    GTGCTGCCAG TGGGTACTGA ACTTCCAGCA GGTACTCTAC CCAGCGAGCA GCTGCCACCT
    TTTCCAGGAC CTTCTCTAAC CCAGTCCCAG CAACCTCTAG AGGATCTTGA TGCTCAATTG
    AGAAGAACAC TTAGTCCAGA GATGATCACA GTGACTTCTG CGGTTGGTCC TGTGTCCATG
    GCGGCTCCAA CAGCAATCAC AGAAGCAGGA ACACAGCCTC AGAAGGGTGT TTCTCAAGTC
    AAAGAAGGCC CTGTCCTAGC AACTAGTTCA GGAGCTGGTG TTTTTAAGAT GGGACGATTT
    CAGGTTTCTG TTGCAGCAGA CGGTGCCCAG AAAGAGGGTA AAAATAAGTC AGAAGATGCA
    AAGTCTGTTC ATTTTGAATC CAGCACCTCA GAGTCCTCAG TGCTATCAAG TAGTAGTCCA
    GAGAGTACCT TGGTGAAACC AGAGCCGAAT GGCATAACCA TCCCTGGTAT CTCTTCAGAT
    GTGCCAGAGA GTGCCCACAA AACTACTGCC TCAGAGGCAA AGTCAGACAC TGGGCAGCCT
    ACCAAGGTTG GACGTTTTCA GGTGACAACT ACAGCAAACA AAGTGGGTCG TTTCTCTGTA
    TCAAAAACTG AGGACAAGAT CACTGACACA AAGAAAGAAG GACCAGTGGC ATCTCCTCCT
    TTTATGGATT TGGAACAAGC TGTTCTTCCT GCTGTGATAC CAAAGAAAGA GAAGCCTGAA
    CTGTCAGAGC CTTCACATCT AAATGGGCCG TCTTCTGACC CGGAGGCCGC TTTTTTAAGT
    AGGGATGTGG ATGATGGTTC CGGTAGTCCA CACTCGCCCC ATCAGCTGAG CTCAAAGAGC
    CTTCCTAGCC AGAATCTAAG TCAAAGCCTT AGTAATTCAT TTAACTCCTC TTACATGAGT
    AGCGACAATG AGTCAGATAT CGAAGATGAA GACTTAAAGT TAGAGCTGCG ACGACTACGA
    GATAAACATC TCAAAGAGAT TCAGGACCTG CAGAGTCGCC AGAAGCATGA AATTGAATCT
    TTGTATACCA AACTGGGCAA GGTGCCCCCT GCTGTTATTA TTCCCCCAGC TGCTCCCCTT
    TCAGGGAGAA GACGACGACC CACTAAAAGC AAAGGCAGCA AATCTAGTCG AAGCAGTTCC
    TTGGGGAATA AAAGCCCCCA GCTTTCAGGT AACCTGTCTG GTCAGAGTGC AGCTTCAGTC
    TTGCACCCCC AGCAGACCCT CCACCCTCCT GGCAACATCC CAGAGTCCGG GCAGAATCAG
    CTGTTACAGC CCCTTAAGCC ATCTCCCTCC AGTGACAACC TCTATTCAGC CTTCACCAGT
    GATGGTGCCA TTTCAGTACC AAGCCTTTCT GCTCCAGGTC AAGGAACCAG CAGCACAAAC
    ACTGTTGGGG CAACAGTGAA CAGCCAAGCC GCCCAAGCTC AGCCTCCTGC CATGACGTCC
    AGCAGGAAGG GCACATTCAC AGATGACTTG CACAAGTTGG TAGACAATTG GGCCCGAGAT
    GCCATGAATC TCTCAGGCAG GAGAGGAAGC AAAGGGCACA TGAATTACGA GGGCCCTGGA
    ATGGCAAGGA AGTTCTCTGC ACCTGGGCAA CTGTGCATCT CCATGACCTC GAACCTGGGT
    GGCTCTGCCC CCATCTCTGC AGCATCAGCT ACCTCTCTAG GTCACTTCAC CAAGTCTATG
    TGCCCCCCAC AGCAGTATGG CTTTCCAGCT ACCCCATTTG GCGCTCAATG GAGTGGGACG
    GGTGGCCCAG CACCACAGCC ACTTGGCCAG TTCCAACCTG TGGGAACTGC CTCCTTGCAG
    AATTTCAACA TCAGCAATTT GCAGAAATCC ATCAGCAACC CCCCAGGCTC CAACCTGCGG
    ACCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28188
    Clone ID Related Accession (Same CDS sequence) NM_018979.3 , NM_018979.4
    Accession Version NM_018979.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-09
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase WNK1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004765.2, AJ296290.1, CD629562.1, AB002342.2, BX644268.1, DA720959.1, AC004803.11, BC141881.1 and AI074334.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_018979.3. Transcript Variant: This variant (1) encodes the most common isoform (1), as indicated in PubMed ID 18521183. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ296290.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000315939.11/ ENSP00000313059.6 RefSeq Select criteria :: based on expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT
    CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AACCCATACA ACATCCTCAG
    CAGCAGCAGG GAATACAGCA GACAGCCCCT CCTCAACAGA CAGTGCAGTA TTCACTTTCA
    CAGACATCAA CCTCCAGTGA GGCCACTACT GCACAGCCAG TGAGTCAGCC TCAAGCTCCA
    CAAGTCTTGC CTCAAGTATC AGCTGGAAAA CAGCTTCCAG TTTCCCAGCC AGTACCAACT
    ATCCAAGGCG AACCTCAGAT CCCAGTTGCG ACACAACCCT CGGTTGTTCC AGTCCACTCT
    GGTGCTCATT TCCTTCCAGT GGGACAGCCG CTCCCTACTC CCTTGCTCCC TCAGTACCCT
    GTCTCTCAGA TTCCCATATC AACTCCTCAT GTGTCTACGG CTCAGACAGG TTTCTCATCC
    CTTCCCATCA CAATGGCAGC TGGCATTACT CAGCCTCTGC TCACGTTGGC TTCATCTGCT
    ACAACAGCTG CGATCCCGGG GGTATCAACT GTGGTTCCTA GTCAGCTTCC AACCCTTCTG
    CAGCCTGTGA CTCAGCTGCC AAGTCAGGTT CACCCACAGC TCCTACAACC AGCAGTTCAG
    TCCATGGGAA TACCAGCTAA CCTTGGACAA GCTGCTGAGG TTCCACTTTC CTCTGGAGAT
    GTTCTGTACC AGGGCTTCCC ACCTCGACTG CCACCACAGT ACCCAGGAGA TTCAAATATT
    GCTCCCTCTT CCAACGTGGC TTCTGTTTGC ATCCATTCTA CAGTCCTATC CCCTCCCATG
    CCGACAGAAG TACTGGCTAC ACCTGGGTAC TTTCCCACAG TGGTGCAGCC TTATGTGGAA
    TCAAATCTTT TAGTTCCTAT GGGTGGTGTA GGAGGACAGG TTCAAGTGTC CCAGCCAGGA
    GGGAGTTTAG CACAAGCCCC CACTACATCC TCCCAGCAAG CAGTTTTGGA GAGTACTCAG
    GGAGTCTCTC AGGTTGCTCC TGCAGAGCCA GTTGCAGTAG CACAGACCCA AGCTACCCAG
    CCGACCACTT TGGCTTCCTC TGTAGACAGT GCACATTCAG ATGTTGCTTC AGGTATGAGT
    GATGGCAATG AGAACGTCCC ATCTTCCAGT GGAAGGCATG AAGGAAGAAC TACAAAACGG
    CATTACCGAA AATCTGTAAG GAGTCGCTCT CGACATGAAA AAACTTCACG CCCAAAATTA
    AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAATT AGAGACTCAT
    AATAGGAAAA TGGTTACATT CAAATTTGAC CTAGATGGTG ACAACCCCGA GGAGATAGCA
    ACAATTATGG TGAACAATGA CTTTATTCTA GCAATAGAGA GAGAGTCGTT TGTGGATCAA
    GTGCGAGAAA TTATTGAAAA AGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA
    GAGGGTGATC AGGGATTGGA GAGTCTACAA GGAAAGGATG ACTATGGCTT TTCAGGTTCT
    CAGAAATTGG AAGGAGAGTT CAAACAACCA ATTCCTGCGT CTTCCATGCC ACAGCAAATA
    GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CGGGAAGGCG GTTTATAGTG
    AGTCCTGTGC CAGAAAGCCG ATTACGAGAA TCAAAAGTTT TCCCCAGTGA AATAACAGAT
    ACAGTTGCTG CCTCTACAGC TCAGAGCCCT GGAATGAACT TGTCTCACTC TGCATCATCC
    CTTAGTCTAC AACAGGCCTT TTCTGAACTT AGACGTGCCC AAATGACAGA AGGACCCAAC
    ACAGCACCTC CAAACTTTAG TCATACAGGA CCAACATTTC CAGTAGTACC TCCTTTCTTA
    AGTAGCATTG CTGGAGTCCC AACCACAGCA GCAGCCACAG CACCAGTCCC TGCAACAAGC
    AGCCCTCCTA ATGACATTTC CACATCAGTA ATTCAGTCTG AGGTTACAGT GCCCACTGAA
    GAGGGGATTG CTGGAGTTGC CACCAGCACA GGTGTGGTAA CTTCAGGTGG TCTCCCCATA
    CCACCTGTGT CTGAATCACC AGTACTTTCC AGCGTAGTTT CAAGTATCAC AATACCTGCA
    GTTGTCTCAA TATCTACTAC ATCCCCGTCA CTTCAAGTCC CCACATCCAC ATCTGAGATC
    GTTGTTTCTA GTACAGCACT GTATCCTTCA GTAACAGTTT CAGCAACTTC AGCCTCTGCA
    GGGGGCAGTA CTGCTACCCC AGGTCCTAAG CCTCCAGCTG TAGTATCTCA GCAGGCAGCA
    GGCAGCACTA CTGTGGGAGC CACATTAACA TCAGTTTCTA CCACCACTTC ATTCCCAAGC
    ACAGCTTCAC AGCTGTGCAT TCAGCTTAGC AGCAGTACTT CTACTCCTAC TTTAGCTGAA
    ACCGTGGTAG TTAGCGCACA CTCACTAGAT AAGACATCTC ATAGCAGTAC AACTGGATTG
    GCTTTCTCCC TCTCTGCACC ATCTTCCTCT TCCTCTCCTG GAGCAGGAGT GTCTAGTTAT
    ATTTCTCAGC CTGGTGGGCT GCATCCTTTG GTCATTCCAT CAGTGATAGC TTCTACTCCT
    ATTCTTCCCC AAGCAGCAGG ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAGTATC
    CCACCCTTGG TACAGCCTGT TGCCAATGTG CCTGCTGTAC AGCAGACACT AATTCATAGT
    CAGCCTCAAC CAGCTTTGCT TCCCAACCAG CCCCATACTC ATTGTCCTGA AGTAGATTCT
    GATACACAAC CCAAAGCTCC TGGAATTGAT GACATAAAGA CTCTAGAAGA AAAGCTGCGG
    TCTCTGTTCA GTGAACACAG CTCATCTGGA GCTCAGCATG CCTCTGTCTC ACTGGAGACC
    TCACTAGTCA TAGAGAGCAC TGTCACACCA GGCATCCCAA CTACTGCTGT TGCACCAAGC
    AAACTCCTGA CTTCTACCAC AAGTACTTGC TTACCACCAA CCAATTTACC ACTAGGAACA
    GTTGCTTTGC CAGTTACACC AGTGGTCACA CCTGGGCAAG TTTCTACCCC AGTCAGCACT
    ACTACATCAG GAGTGAAACC TGGAACTGCT CCCTCCAAGC CACCTCTAAC TAAGGCTCCG
    GTGCTGCCAG TGGGTACTGA ACTTCCAGCA GGTACTCTAC CCAGCGAGCA GCTGCCACCT
    TTTCCAGGAC CTTCTCTAAC CCAGTCCCAG CAACCTCTAG AGGATCTTGA TGCTCAATTG
    AGAAGAACAC TTAGTCCAGA GATGATCACA GTGACTTCTG CGGTTGGTCC TGTGTCCATG
    GCGGCTCCAA CAGCAATCAC AGAAGCAGGA ACACAGCCTC AGAAGGGTGT TTCTCAAGTC
    AAAGAAGGCC CTGTCCTAGC AACTAGTTCA GGAGCTGGTG TTTTTAAGAT GGGACGATTT
    CAGGTTTCTG TTGCAGCAGA CGGTGCCCAG AAAGAGGGTA AAAATAAGTC AGAAGATGCA
    AAGTCTGTTC ATTTTGAATC CAGCACCTCA GAGTCCTCAG TGCTATCAAG TAGTAGTCCA
    GAGAGTACCT TGGTGAAACC AGAGCCGAAT GGCATAACCA TCCCTGGTAT CTCTTCAGAT
    GTGCCAGAGA GTGCCCACAA AACTACTGCC TCAGAGGCAA AGTCAGACAC TGGGCAGCCT
    ACCAAGGTTG GACGTTTTCA GGTGACAACT ACAGCAAACA AAGTGGGTCG TTTCTCTGTA
    TCAAAAACTG AGGACAAGAT CACTGACACA AAGAAAGAAG GACCAGTGGC ATCTCCTCCT
    TTTATGGATT TGGAACAAGC TGTTCTTCCT GCTGTGATAC CAAAGAAAGA GAAGCCTGAA
    CTGTCAGAGC CTTCACATCT AAATGGGCCG TCTTCTGACC CGGAGGCCGC TTTTTTAAGT
    AGGGATGTGG ATGATGGTTC CGGTAGTCCA CACTCGCCCC ATCAGCTGAG CTCAAAGAGC
    CTTCCTAGCC AGAATCTAAG TCAAAGCCTT AGTAATTCAT TTAACTCCTC TTACATGAGT
    AGCGACAATG AGTCAGATAT CGAAGATGAA GACTTAAAGT TAGAGCTGCG ACGACTACGA
    GATAAACATC TCAAAGAGAT TCAGGACCTG CAGAGTCGCC AGAAGCATGA AATTGAATCT
    TTGTATACCA AACTGGGCAA GGTGCCCCCT GCTGTTATTA TTCCCCCAGC TGCTCCCCTT
    TCAGGGAGAA GACGACGACC CACTAAAAGC AAAGGCAGCA AATCTAGTCG AAGCAGTTCC
    TTGGGGAATA AAAGCCCCCA GCTTTCAGGT AACCTGTCTG GTCAGAGTGC AGCTTCAGTC
    TTGCACCCCC AGCAGACCCT CCACCCTCCT GGCAACATCC CAGAGTCCGG GCAGAATCAG
    CTGTTACAGC CCCTTAAGCC ATCTCCCTCC AGTGACAACC TCTATTCAGC CTTCACCAGT
    GATGGTGCCA TTTCAGTACC AAGCCTTTCT GCTCCAGGTC AAGGAACCAG CAGCACAAAC
    ACTGTTGGGG CAACAGTGAA CAGCCAAGCC GCCCAAGCTC AGCCTCCTGC CATGACGTCC
    AGCAGGAAGG GCACATTCAC AGATGACTTG CACAAGTTGG TAGACAATTG GGCCCGAGAT
    GCCATGAATC TCTCAGGCAG GAGAGGAAGC AAAGGGCACA TGAATTACGA GGGCCCTGGA
    ATGGCAAGGA AGTTCTCTGC ACCTGGGCAA CTGTGCATCT CCATGACCTC GAACCTGGGT
    GGCTCTGCCC CCATCTCTGC AGCATCAGCT ACCTCTCTAG GTCACTTCAC CAAGTCTATG
    TGCCCCCCAC AGCAGTATGG CTTTCCAGCT ACCCCATTTG GCGCTCAATG GAGTGGGACG
    GGTGGCCCAG CACCACAGCC ACTTGGCCAG TTCCAACCTG TGGGAACTGC CTCCTTGCAG
    AATTTCAACA TCAGCAATTT GCAGAAATCC ATCAGCAACC CCCCAGGCTC CAACCTGCGG
    ACCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_061852.3
    CDS988..8136
    Translation

    Target ORF information:

    RefSeq Version NM_018979.4
    Organism Homo sapiens(human)
    Definition Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_018979.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT
    CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AACCCATACA ACATCCTCAG
    CAGCAGCAGG GAATACAGCA GACAGCCCCT CCTCAACAGA CAGTGCAGTA TTCACTTTCA
    CAGACATCAA CCTCCAGTGA GGCCACTACT GCACAGCCAG TGAGTCAGCC TCAAGCTCCA
    CAAGTCTTGC CTCAAGTATC AGCTGGAAAA CAGCTTCCAG TTTCCCAGCC AGTACCAACT
    ATCCAAGGCG AACCTCAGAT CCCAGTTGCG ACACAACCCT CGGTTGTTCC AGTCCACTCT
    GGTGCTCATT TCCTTCCAGT GGGACAGCCG CTCCCTACTC CCTTGCTCCC TCAGTACCCT
    GTCTCTCAGA TTCCCATATC AACTCCTCAT GTGTCTACGG CTCAGACAGG TTTCTCATCC
    CTTCCCATCA CAATGGCAGC TGGCATTACT CAGCCTCTGC TCACGTTGGC TTCATCTGCT
    ACAACAGCTG CGATCCCGGG GGTATCAACT GTGGTTCCTA GTCAGCTTCC AACCCTTCTG
    CAGCCTGTGA CTCAGCTGCC AAGTCAGGTT CACCCACAGC TCCTACAACC AGCAGTTCAG
    TCCATGGGAA TACCAGCTAA CCTTGGACAA GCTGCTGAGG TTCCACTTTC CTCTGGAGAT
    GTTCTGTACC AGGGCTTCCC ACCTCGACTG CCACCACAGT ACCCAGGAGA TTCAAATATT
    GCTCCCTCTT CCAACGTGGC TTCTGTTTGC ATCCATTCTA CAGTCCTATC CCCTCCCATG
    CCGACAGAAG TACTGGCTAC ACCTGGGTAC TTTCCCACAG TGGTGCAGCC TTATGTGGAA
    TCAAATCTTT TAGTTCCTAT GGGTGGTGTA GGAGGACAGG TTCAAGTGTC CCAGCCAGGA
    GGGAGTTTAG CACAAGCCCC CACTACATCC TCCCAGCAAG CAGTTTTGGA GAGTACTCAG
    GGAGTCTCTC AGGTTGCTCC TGCAGAGCCA GTTGCAGTAG CACAGACCCA AGCTACCCAG
    CCGACCACTT TGGCTTCCTC TGTAGACAGT GCACATTCAG ATGTTGCTTC AGGTATGAGT
    GATGGCAATG AGAACGTCCC ATCTTCCAGT GGAAGGCATG AAGGAAGAAC TACAAAACGG
    CATTACCGAA AATCTGTAAG GAGTCGCTCT CGACATGAAA AAACTTCACG CCCAAAATTA
    AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAATT AGAGACTCAT
    AATAGGAAAA TGGTTACATT CAAATTTGAC CTAGATGGTG ACAACCCCGA GGAGATAGCA
    ACAATTATGG TGAACAATGA CTTTATTCTA GCAATAGAGA GAGAGTCGTT TGTGGATCAA
    GTGCGAGAAA TTATTGAAAA AGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA
    GAGGGTGATC AGGGATTGGA GAGTCTACAA GGAAAGGATG ACTATGGCTT TTCAGGTTCT
    CAGAAATTGG AAGGAGAGTT CAAACAACCA ATTCCTGCGT CTTCCATGCC ACAGCAAATA
    GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CGGGAAGGCG GTTTATAGTG
    AGTCCTGTGC CAGAAAGCCG ATTACGAGAA TCAAAAGTTT TCCCCAGTGA AATAACAGAT
    ACAGTTGCTG CCTCTACAGC TCAGAGCCCT GGAATGAACT TGTCTCACTC TGCATCATCC
    CTTAGTCTAC AACAGGCCTT TTCTGAACTT AGACGTGCCC AAATGACAGA AGGACCCAAC
    ACAGCACCTC CAAACTTTAG TCATACAGGA CCAACATTTC CAGTAGTACC TCCTTTCTTA
    AGTAGCATTG CTGGAGTCCC AACCACAGCA GCAGCCACAG CACCAGTCCC TGCAACAAGC
    AGCCCTCCTA ATGACATTTC CACATCAGTA ATTCAGTCTG AGGTTACAGT GCCCACTGAA
    GAGGGGATTG CTGGAGTTGC CACCAGCACA GGTGTGGTAA CTTCAGGTGG TCTCCCCATA
    CCACCTGTGT CTGAATCACC AGTACTTTCC AGCGTAGTTT CAAGTATCAC AATACCTGCA
    GTTGTCTCAA TATCTACTAC ATCCCCGTCA CTTCAAGTCC CCACATCCAC ATCTGAGATC
    GTTGTTTCTA GTACAGCACT GTATCCTTCA GTAACAGTTT CAGCAACTTC AGCCTCTGCA
    GGGGGCAGTA CTGCTACCCC AGGTCCTAAG CCTCCAGCTG TAGTATCTCA GCAGGCAGCA
    GGCAGCACTA CTGTGGGAGC CACATTAACA TCAGTTTCTA CCACCACTTC ATTCCCAAGC
    ACAGCTTCAC AGCTGTGCAT TCAGCTTAGC AGCAGTACTT CTACTCCTAC TTTAGCTGAA
    ACCGTGGTAG TTAGCGCACA CTCACTAGAT AAGACATCTC ATAGCAGTAC AACTGGATTG
    GCTTTCTCCC TCTCTGCACC ATCTTCCTCT TCCTCTCCTG GAGCAGGAGT GTCTAGTTAT
    ATTTCTCAGC CTGGTGGGCT GCATCCTTTG GTCATTCCAT CAGTGATAGC TTCTACTCCT
    ATTCTTCCCC AAGCAGCAGG ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAGTATC
    CCACCCTTGG TACAGCCTGT TGCCAATGTG CCTGCTGTAC AGCAGACACT AATTCATAGT
    CAGCCTCAAC CAGCTTTGCT TCCCAACCAG CCCCATACTC ATTGTCCTGA AGTAGATTCT
    GATACACAAC CCAAAGCTCC TGGAATTGAT GACATAAAGA CTCTAGAAGA AAAGCTGCGG
    TCTCTGTTCA GTGAACACAG CTCATCTGGA GCTCAGCATG CCTCTGTCTC ACTGGAGACC
    TCACTAGTCA TAGAGAGCAC TGTCACACCA GGCATCCCAA CTACTGCTGT TGCACCAAGC
    AAACTCCTGA CTTCTACCAC AAGTACTTGC TTACCACCAA CCAATTTACC ACTAGGAACA
    GTTGCTTTGC CAGTTACACC AGTGGTCACA CCTGGGCAAG TTTCTACCCC AGTCAGCACT
    ACTACATCAG GAGTGAAACC TGGAACTGCT CCCTCCAAGC CACCTCTAAC TAAGGCTCCG
    GTGCTGCCAG TGGGTACTGA ACTTCCAGCA GGTACTCTAC CCAGCGAGCA GCTGCCACCT
    TTTCCAGGAC CTTCTCTAAC CCAGTCCCAG CAACCTCTAG AGGATCTTGA TGCTCAATTG
    AGAAGAACAC TTAGTCCAGA GATGATCACA GTGACTTCTG CGGTTGGTCC TGTGTCCATG
    GCGGCTCCAA CAGCAATCAC AGAAGCAGGA ACACAGCCTC AGAAGGGTGT TTCTCAAGTC
    AAAGAAGGCC CTGTCCTAGC AACTAGTTCA GGAGCTGGTG TTTTTAAGAT GGGACGATTT
    CAGGTTTCTG TTGCAGCAGA CGGTGCCCAG AAAGAGGGTA AAAATAAGTC AGAAGATGCA
    AAGTCTGTTC ATTTTGAATC CAGCACCTCA GAGTCCTCAG TGCTATCAAG TAGTAGTCCA
    GAGAGTACCT TGGTGAAACC AGAGCCGAAT GGCATAACCA TCCCTGGTAT CTCTTCAGAT
    GTGCCAGAGA GTGCCCACAA AACTACTGCC TCAGAGGCAA AGTCAGACAC TGGGCAGCCT
    ACCAAGGTTG GACGTTTTCA GGTGACAACT ACAGCAAACA AAGTGGGTCG TTTCTCTGTA
    TCAAAAACTG AGGACAAGAT CACTGACACA AAGAAAGAAG GACCAGTGGC ATCTCCTCCT
    TTTATGGATT TGGAACAAGC TGTTCTTCCT GCTGTGATAC CAAAGAAAGA GAAGCCTGAA
    CTGTCAGAGC CTTCACATCT AAATGGGCCG TCTTCTGACC CGGAGGCCGC TTTTTTAAGT
    AGGGATGTGG ATGATGGTTC CGGTAGTCCA CACTCGCCCC ATCAGCTGAG CTCAAAGAGC
    CTTCCTAGCC AGAATCTAAG TCAAAGCCTT AGTAATTCAT TTAACTCCTC TTACATGAGT
    AGCGACAATG AGTCAGATAT CGAAGATGAA GACTTAAAGT TAGAGCTGCG ACGACTACGA
    GATAAACATC TCAAAGAGAT TCAGGACCTG CAGAGTCGCC AGAAGCATGA AATTGAATCT
    TTGTATACCA AACTGGGCAA GGTGCCCCCT GCTGTTATTA TTCCCCCAGC TGCTCCCCTT
    TCAGGGAGAA GACGACGACC CACTAAAAGC AAAGGCAGCA AATCTAGTCG AAGCAGTTCC
    TTGGGGAATA AAAGCCCCCA GCTTTCAGGT AACCTGTCTG GTCAGAGTGC AGCTTCAGTC
    TTGCACCCCC AGCAGACCCT CCACCCTCCT GGCAACATCC CAGAGTCCGG GCAGAATCAG
    CTGTTACAGC CCCTTAAGCC ATCTCCCTCC AGTGACAACC TCTATTCAGC CTTCACCAGT
    GATGGTGCCA TTTCAGTACC AAGCCTTTCT GCTCCAGGTC AAGGAACCAG CAGCACAAAC
    ACTGTTGGGG CAACAGTGAA CAGCCAAGCC GCCCAAGCTC AGCCTCCTGC CATGACGTCC
    AGCAGGAAGG GCACATTCAC AGATGACTTG CACAAGTTGG TAGACAATTG GGCCCGAGAT
    GCCATGAATC TCTCAGGCAG GAGAGGAAGC AAAGGGCACA TGAATTACGA GGGCCCTGGA
    ATGGCAAGGA AGTTCTCTGC ACCTGGGCAA CTGTGCATCT CCATGACCTC GAACCTGGGT
    GGCTCTGCCC CCATCTCTGC AGCATCAGCT ACCTCTCTAG GTCACTTCAC CAAGTCTATG
    TGCCCCCCAC AGCAGTATGG CTTTCCAGCT ACCCCATTTG GCGCTCAATG GAGTGGGACG
    GGTGGCCCAG CACCACAGCC ACTTGGCCAG TTCCAACCTG TGGGAACTGC CTCCTTGCAG
    AATTTCAACA TCAGCAATTT GCAGAAATCC ATCAGCAACC CCCCAGGCTC CAACCTGCGG
    ACCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu46009
    Clone ID Related Accession (Same CDS sequence) XM_006719003.2
    Accession Version XM_006719003.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7146bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase WNK1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006719003.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT
    CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AACCCATACA ACATCCTCAG
    CAGCAGGGAA TACAGCAGAC AGCCCCTCCT CAACAGACAG TGCAGTATTC ACTTTCACAG
    ACATCAACCT CCAGTGAGGC CACTACTGCA CAGCCAGTGA GTCAGCCTCA AGCTCCACAA
    GTCTTGCCTC AAGTATCAGC TGGAAAACAG CTTCCAGTTT CCCAGCCAGT ACCAACTATC
    CAAGGCGAAC CTCAGATCCC AGTTGCGACA CAACCCTCGG TTGTTCCAGT CCACTCTGGT
    GCTCATTTCC TTCCAGTGGG ACAGCCGCTC CCTACTCCCT TGCTCCCTCA GTACCCTGTC
    TCTCAGATTC CCATATCAAC TCCTCATGTG TCTACGGCTC AGACAGGTTT CTCATCCCTT
    CCCATCACAA TGGCAGCTGG CATTACTCAG CCTCTGCTCA CGTTGGCTTC ATCTGCTACA
    ACAGCTGCGA TCCCGGGGGT ATCAACTGTG GTTCCTAGTC AGCTTCCAAC CCTTCTGCAG
    CCTGTGACTC AGCTGCCAAG TCAGGTTCAC CCACAGCTCC TACAACCAGC AGTTCAGTCC
    ATGGGAATAC CAGCTAACCT TGGACAAGCT GCTGAGGTTC CACTTTCCTC TGGAGATGTT
    CTGTACCAGG GCTTCCCACC TCGACTGCCA CCACAGTACC CAGGAGATTC AAATATTGCT
    CCCTCTTCCA ACGTGGCTTC TGTTTGCATC CATTCTACAG TCCTATCCCC TCCCATGCCG
    ACAGAAGTAC TGGCTACACC TGGGTACTTT CCCACAGTGG TGCAGCCTTA TGTGGAATCA
    AATCTTTTAG TTCCTATGGG TGGTGTAGGA GGACAGGTTC AAGTGTCCCA GCCAGGAGGG
    AGTTTAGCAC AAGCCCCCAC TACATCCTCC CAGCAAGCAG TTTTGGAGAG TACTCAGGGA
    GTCTCTCAGG TTGCTCCTGC AGAGCCAGTT GCAGTAGCAC AGACCCAAGC TACCCAGCCG
    ACCACTTTGG CTTCCTCTGT AGACAGTGCA CATTCAGATG TTGCTTCAGG TATGAGTGAT
    GGCAATGAGA ACGTCCCATC TTCCAGTGGA AGGCATGAAG GAAGAACTAC AAAACGGCAT
    TACCGAAAAT CTGTAAGGAG TCGCTCTCGA CATGAAAAAA CTTCACGCCC AAAATTAAGA
    ATTTTGAATG TTTCAAATAA AGGAGACCGA GTAGTAGAAT GTCAATTAGA GACTCATAAT
    AGGAAAATGG TTACATTCAA ATTTGACCTA GATGGTGACA ACCCCGAGGA GATAGCAACA
    ATTATGGTGA ACAATGACTT TATTCTAGCA ATAGAGAGAG AGTCGTTTGT GGATCAAGTG
    CGAGAAATTA TTGAAAAAGC TGATGAAATG CTCAGTGAGG ATGTCAGTGT GGAACCAGAG
    GGTGATCAGG GATTGGAGAG TCTACAAGGA AAGGATGACT ATGGCTTTTC AGGTTCTCAG
    AAATTGGAAG GAGAGTTCAA ACAACCAATT CCTGCGTCTT CCATGCCACA GCAAATAGGC
    ATTCCTACCA GTTCTTTAAC TCAAGTTGTT CATTCTGCGG GAAGGCGGTT TATAGTGAGT
    CCTGTGCCAG AAAGCCGATT ACGAGAATCA AAAGTTTTCC CCAGTGAAAT AACAGATACA
    GTTGCTGCCT CTACAGCTCA GAGCCCTGGA ATGAACTTGT CTCACTCTGC ATCATCCCTT
    AGTCTACAAC AGGCCTTTTC TGAACTTAGA CGTGCCCAAA TGACAGAAGG ACCCAACACA
    GCACCTCCAA ACTTTAGTCA TACAGGACCA ACATTTCCAG TAGTACCTCC TTTCTTAAGT
    AGCATTGCTG GAGTCCCAAC CACAGCAGCA GCCACAGCAC CAGTCCCTGC AACAAGCAGC
    CCTCCTAATG ACATTTCCAC ATCAGTAATT CAGTCTGAGG TTACAGTGCC CACTGAAGAG
    GGGATTGCTG GAGTTGCCAC CAGCACAGGT GTGGTAACTT CAGGTGGTCT CCCCATACCA
    CCTGTGTCTG AATCACCAGT ACTTTCCAGC GTAGTTTCAA GTATCACAAT ACCTGCAGTT
    GTCTCAATAT CTACTACATC CCCGTCACTT CAAGTCCCCA CATCCACATC TGAGATCGTT
    GTTTCTAGTA CAGCACTGTA TCCTTCAGTA ACAGTTTCAG CAACTTCAGC CTCTGCAGGG
    GGCAGTACTG CTACCCCAGG TCCTAAGCCT CCAGCTGTAG TATCTCAGCA GGCAGCAGGC
    AGCACTACTG TGGGAGCCAC ATTAACATCA GTTTCTACCA CCACTTCATT CCCAAGCACA
    GCTTCACAGC TGTGCATTCA GCTTAGCAGC AGTACTTCTA CTCCTACTTT AGCTGAAACC
    GTGGTAGTTA GCGCACACTC ACTAGATAAG ACATCTCATA GCAGTACAAC TGGATTGGCT
    TTCTCCCTCT CTGCACCATC TTCCTCTTCC TCTCCTGGAG CAGGAGTGTC TAGTTATATT
    TCTCAGCCTG GTGGGCTGCA TCCTTTGGTC ATTCCATCAG TGATAGCTTC TACTCCTATT
    CTTCCCCAAG CAGCAGGACC TACTTCTACA CCTTTATTAC CCCAAGTACC TAGTATCCCA
    CCCTTGGTAC AGCCTGTTGC CAATGTGCCT GCTGTACAGC AGACACTAAT TCATAGTCAG
    CCTCAACCAG CTTTGCTTCC CAACCAGCCC CATACTCATT GTCCTGAAGT AGATTCTGAT
    ACACAACCCA AAGCTCCTGG AATTGATGAC ATAAAGACTC TAGAAGAAAA GCTGCGGTCT
    CTGTTCAGTG AACACAGCTC ATCTGGAGCT CAGCATGCCT CTGTCTCACT GGAGACCTCA
    CTAGTCATAG AGAGCACTGT CACACCAGGC ATCCCAACTA CTGCTGTTGC ACCAAGCAAA
    CTCCTGACTT CTACCACAAG TACTTGCTTA CCACCAACCA ATTTACCACT AGGAACAGTT
    GCTTTGCCAG TTACACCAGT GGTCACACCT GGGCAAGTTT CTACCCCAGT CAGCACTACT
    ACATCAGGAG TGAAACCTGG AACTGCTCCC TCCAAGCCAC CTCTAACTAA GGCTCCGGTG
    CTGCCAGTGG GTACTGAACT TCCAGCAGGT ACTCTACCCA GCGAGCAGCT GCCACCTTTT
    CCAGGACCTT CTCTAACCCA GTCCCAGCAA CCTCTAGAGG ATCTTGATGC TCAATTGAGA
    AGAACACTTA GTCCAGAGAT GATCACAGTG ACTTCTGCGG TTGGTCCTGT GTCCATGGCG
    GCTCCAACAG CAATCACAGA AGCAGGAACA CAGCCTCAGA AGGGTGTTTC TCAAGTCAAA
    GAAGGCCCTG TCCTAGCAAC TAGTTCAGGA GCTGGTGTTT TTAAGATGGG ACGATTTCAG
    GTTTCTGTTG CAGCAGACGG TGCCCAGAAA GAGGGTAAAA ATAAGTCAGA AGATGCAAAG
    TCTGTTCATT TTGAATCCAG CACCTCAGAG TCCTCAGTGC TATCAAGTAG TAGTCCAGAG
    AGTACCTTGG TGAAACCAGA GCCGAATGGC ATAACCATCC CTGGTATCTC TTCAGATGTG
    CCAGAGAGTG CCCACAAAAC TACTGCCTCA GAGGCAAAGT CAGACACTGG GCAGCCTACC
    AAGGTTGGAC GTTTTCAGGT GACAACTACA GCAAACAAAG TGGGTCGTTT CTCTGTATCA
    AAAACTGAGG ACAAGATCAC TGACACAAAG AAAGAAGGAC CAGTGGCATC TCCTCCTTTT
    ATGGATTTGG AACAAGCTGT TCTTCCTGCT GTGATACCAA AGAAAGAGAA GCCTGAACTG
    TCAGAGCCTT CACATCTAAA TGGGCCGTCT TCTGACCCGG AGGCCGCTTT TTTAAGTAGG
    GATGTGGATG ATGGTTCCGG TAGTCCACAC TCGCCCCATC AGCTGAGCTC AAAGAGCCTT
    CCTAGCCAGA ATCTAAGTCA AAGCCTTAGT AATTCATTTA ACTCCTCTTA CATGAGTAGC
    GACAATGAGT CAGATATCGA AGATGAAGAC TTAAAGTTAG AGCTGCGACG ACTACGAGAT
    AAACATCTCA AAGAGATTCA GGACCTGCAG AGTCGCCAGA AGCATGAAAT TGAATCTTTG
    TATACCAAAC TGGGCAAGGT GCCCCCTGCT GTTATTATTC CCCCAGCTGC TCCCCTTTCA
    GGGAGAAGAC GACGACCCAC TAAAAGCAAA GGCAGCAAAT CTAGTCGAAG CAGTTCCTTG
    GGGAATAAAA GCCCCCAGCT TTCAGGTAAC CTGTCTGGTC AGAGTGCAGC TTCAGTCTTG
    CACCCCCAGC AGACCCTCCA CCCTCCTGGC AACATCCCAG AGTCCGGGCA GAATCAGCTG
    TTACAGCCCC TTAAGCCATC TCCCTCCAGT GACAACCTCT ATTCAGCCTT CACCAGTGAT
    GGTGCCATTT CAGTACCAAG CCTTTCTGCT CCAGGTCAAG GAACCAGCAG CACAAACACT
    GTTGGGGCAA CAGTGAACAG CCAAGCCGCC CAAGCTCAGC CTCCTGCCAT GACGTCCAGC
    AGGAAGGGCA CATTCACAGA TGACTTGCAC AAGTTGGTAG ACAATTGGGC CCGAGATGCC
    ATGAATCTCT CAGGCAGGAG AGGAAGCAAA GGGCACATGA ATTACGAGGG CCCTGGAATG
    GCAAGGAAGT TCTCTGCACC TGGGCAACTG TGCATCTCCA TGACCTCGAA CCTGGGTGGC
    TCTGCCCCCA TCTCTGCAGC ATCAGCTACC TCTCTAGGTC ACTTCACCAA GTCTATGTGC
    CCCCCACAGC AGTATGGCTT TCCAGCTACC CCATTTGGCG CTCAATGGAG TGGGACGGGT
    GGCCCAGCAC CACAGCCACT TGGCCAGTTC CAACCTGTGG GAACTGCCTC CTTGCAGAAT
    TTCAACATCA GCAATTTGCA GAAATCCATC AGCAACCCCC CAGGCTCCAA CCTGCGGACC
    ACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006719066.1
    CDS969..8114
    Translation

    Target ORF information:

    RefSeq Version XM_006719003.2
    Organism Homo sapiens(human)
    Definition Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006719003.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT
    CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AACCCATACA ACATCCTCAG
    CAGCAGGGAA TACAGCAGAC AGCCCCTCCT CAACAGACAG TGCAGTATTC ACTTTCACAG
    ACATCAACCT CCAGTGAGGC CACTACTGCA CAGCCAGTGA GTCAGCCTCA AGCTCCACAA
    GTCTTGCCTC AAGTATCAGC TGGAAAACAG CTTCCAGTTT CCCAGCCAGT ACCAACTATC
    CAAGGCGAAC CTCAGATCCC AGTTGCGACA CAACCCTCGG TTGTTCCAGT CCACTCTGGT
    GCTCATTTCC TTCCAGTGGG ACAGCCGCTC CCTACTCCCT TGCTCCCTCA GTACCCTGTC
    TCTCAGATTC CCATATCAAC TCCTCATGTG TCTACGGCTC AGACAGGTTT CTCATCCCTT
    CCCATCACAA TGGCAGCTGG CATTACTCAG CCTCTGCTCA CGTTGGCTTC ATCTGCTACA
    ACAGCTGCGA TCCCGGGGGT ATCAACTGTG GTTCCTAGTC AGCTTCCAAC CCTTCTGCAG
    CCTGTGACTC AGCTGCCAAG TCAGGTTCAC CCACAGCTCC TACAACCAGC AGTTCAGTCC
    ATGGGAATAC CAGCTAACCT TGGACAAGCT GCTGAGGTTC CACTTTCCTC TGGAGATGTT
    CTGTACCAGG GCTTCCCACC TCGACTGCCA CCACAGTACC CAGGAGATTC AAATATTGCT
    CCCTCTTCCA ACGTGGCTTC TGTTTGCATC CATTCTACAG TCCTATCCCC TCCCATGCCG
    ACAGAAGTAC TGGCTACACC TGGGTACTTT CCCACAGTGG TGCAGCCTTA TGTGGAATCA
    AATCTTTTAG TTCCTATGGG TGGTGTAGGA GGACAGGTTC AAGTGTCCCA GCCAGGAGGG
    AGTTTAGCAC AAGCCCCCAC TACATCCTCC CAGCAAGCAG TTTTGGAGAG TACTCAGGGA
    GTCTCTCAGG TTGCTCCTGC AGAGCCAGTT GCAGTAGCAC AGACCCAAGC TACCCAGCCG
    ACCACTTTGG CTTCCTCTGT AGACAGTGCA CATTCAGATG TTGCTTCAGG TATGAGTGAT
    GGCAATGAGA ACGTCCCATC TTCCAGTGGA AGGCATGAAG GAAGAACTAC AAAACGGCAT
    TACCGAAAAT CTGTAAGGAG TCGCTCTCGA CATGAAAAAA CTTCACGCCC AAAATTAAGA
    ATTTTGAATG TTTCAAATAA AGGAGACCGA GTAGTAGAAT GTCAATTAGA GACTCATAAT
    AGGAAAATGG TTACATTCAA ATTTGACCTA GATGGTGACA ACCCCGAGGA GATAGCAACA
    ATTATGGTGA ACAATGACTT TATTCTAGCA ATAGAGAGAG AGTCGTTTGT GGATCAAGTG
    CGAGAAATTA TTGAAAAAGC TGATGAAATG CTCAGTGAGG ATGTCAGTGT GGAACCAGAG
    GGTGATCAGG GATTGGAGAG TCTACAAGGA AAGGATGACT ATGGCTTTTC AGGTTCTCAG
    AAATTGGAAG GAGAGTTCAA ACAACCAATT CCTGCGTCTT CCATGCCACA GCAAATAGGC
    ATTCCTACCA GTTCTTTAAC TCAAGTTGTT CATTCTGCGG GAAGGCGGTT TATAGTGAGT
    CCTGTGCCAG AAAGCCGATT ACGAGAATCA AAAGTTTTCC CCAGTGAAAT AACAGATACA
    GTTGCTGCCT CTACAGCTCA GAGCCCTGGA ATGAACTTGT CTCACTCTGC ATCATCCCTT
    AGTCTACAAC AGGCCTTTTC TGAACTTAGA CGTGCCCAAA TGACAGAAGG ACCCAACACA
    GCACCTCCAA ACTTTAGTCA TACAGGACCA ACATTTCCAG TAGTACCTCC TTTCTTAAGT
    AGCATTGCTG GAGTCCCAAC CACAGCAGCA GCCACAGCAC CAGTCCCTGC AACAAGCAGC
    CCTCCTAATG ACATTTCCAC ATCAGTAATT CAGTCTGAGG TTACAGTGCC CACTGAAGAG
    GGGATTGCTG GAGTTGCCAC CAGCACAGGT GTGGTAACTT CAGGTGGTCT CCCCATACCA
    CCTGTGTCTG AATCACCAGT ACTTTCCAGC GTAGTTTCAA GTATCACAAT ACCTGCAGTT
    GTCTCAATAT CTACTACATC CCCGTCACTT CAAGTCCCCA CATCCACATC TGAGATCGTT
    GTTTCTAGTA CAGCACTGTA TCCTTCAGTA ACAGTTTCAG CAACTTCAGC CTCTGCAGGG
    GGCAGTACTG CTACCCCAGG TCCTAAGCCT CCAGCTGTAG TATCTCAGCA GGCAGCAGGC
    AGCACTACTG TGGGAGCCAC ATTAACATCA GTTTCTACCA CCACTTCATT CCCAAGCACA
    GCTTCACAGC TGTGCATTCA GCTTAGCAGC AGTACTTCTA CTCCTACTTT AGCTGAAACC
    GTGGTAGTTA GCGCACACTC ACTAGATAAG ACATCTCATA GCAGTACAAC TGGATTGGCT
    TTCTCCCTCT CTGCACCATC TTCCTCTTCC TCTCCTGGAG CAGGAGTGTC TAGTTATATT
    TCTCAGCCTG GTGGGCTGCA TCCTTTGGTC ATTCCATCAG TGATAGCTTC TACTCCTATT
    CTTCCCCAAG CAGCAGGACC TACTTCTACA CCTTTATTAC CCCAAGTACC TAGTATCCCA
    CCCTTGGTAC AGCCTGTTGC CAATGTGCCT GCTGTACAGC AGACACTAAT TCATAGTCAG
    CCTCAACCAG CTTTGCTTCC CAACCAGCCC CATACTCATT GTCCTGAAGT AGATTCTGAT
    ACACAACCCA AAGCTCCTGG AATTGATGAC ATAAAGACTC TAGAAGAAAA GCTGCGGTCT
    CTGTTCAGTG AACACAGCTC ATCTGGAGCT CAGCATGCCT CTGTCTCACT GGAGACCTCA
    CTAGTCATAG AGAGCACTGT CACACCAGGC ATCCCAACTA CTGCTGTTGC ACCAAGCAAA
    CTCCTGACTT CTACCACAAG TACTTGCTTA CCACCAACCA ATTTACCACT AGGAACAGTT
    GCTTTGCCAG TTACACCAGT GGTCACACCT GGGCAAGTTT CTACCCCAGT CAGCACTACT
    ACATCAGGAG TGAAACCTGG AACTGCTCCC TCCAAGCCAC CTCTAACTAA GGCTCCGGTG
    CTGCCAGTGG GTACTGAACT TCCAGCAGGT ACTCTACCCA GCGAGCAGCT GCCACCTTTT
    CCAGGACCTT CTCTAACCCA GTCCCAGCAA CCTCTAGAGG ATCTTGATGC TCAATTGAGA
    AGAACACTTA GTCCAGAGAT GATCACAGTG ACTTCTGCGG TTGGTCCTGT GTCCATGGCG
    GCTCCAACAG CAATCACAGA AGCAGGAACA CAGCCTCAGA AGGGTGTTTC TCAAGTCAAA
    GAAGGCCCTG TCCTAGCAAC TAGTTCAGGA GCTGGTGTTT TTAAGATGGG ACGATTTCAG
    GTTTCTGTTG CAGCAGACGG TGCCCAGAAA GAGGGTAAAA ATAAGTCAGA AGATGCAAAG
    TCTGTTCATT TTGAATCCAG CACCTCAGAG TCCTCAGTGC TATCAAGTAG TAGTCCAGAG
    AGTACCTTGG TGAAACCAGA GCCGAATGGC ATAACCATCC CTGGTATCTC TTCAGATGTG
    CCAGAGAGTG CCCACAAAAC TACTGCCTCA GAGGCAAAGT CAGACACTGG GCAGCCTACC
    AAGGTTGGAC GTTTTCAGGT GACAACTACA GCAAACAAAG TGGGTCGTTT CTCTGTATCA
    AAAACTGAGG ACAAGATCAC TGACACAAAG AAAGAAGGAC CAGTGGCATC TCCTCCTTTT
    ATGGATTTGG AACAAGCTGT TCTTCCTGCT GTGATACCAA AGAAAGAGAA GCCTGAACTG
    TCAGAGCCTT CACATCTAAA TGGGCCGTCT TCTGACCCGG AGGCCGCTTT TTTAAGTAGG
    GATGTGGATG ATGGTTCCGG TAGTCCACAC TCGCCCCATC AGCTGAGCTC AAAGAGCCTT
    CCTAGCCAGA ATCTAAGTCA AAGCCTTAGT AATTCATTTA ACTCCTCTTA CATGAGTAGC
    GACAATGAGT CAGATATCGA AGATGAAGAC TTAAAGTTAG AGCTGCGACG ACTACGAGAT
    AAACATCTCA AAGAGATTCA GGACCTGCAG AGTCGCCAGA AGCATGAAAT TGAATCTTTG
    TATACCAAAC TGGGCAAGGT GCCCCCTGCT GTTATTATTC CCCCAGCTGC TCCCCTTTCA
    GGGAGAAGAC GACGACCCAC TAAAAGCAAA GGCAGCAAAT CTAGTCGAAG CAGTTCCTTG
    GGGAATAAAA GCCCCCAGCT TTCAGGTAAC CTGTCTGGTC AGAGTGCAGC TTCAGTCTTG
    CACCCCCAGC AGACCCTCCA CCCTCCTGGC AACATCCCAG AGTCCGGGCA GAATCAGCTG
    TTACAGCCCC TTAAGCCATC TCCCTCCAGT GACAACCTCT ATTCAGCCTT CACCAGTGAT
    GGTGCCATTT CAGTACCAAG CCTTTCTGCT CCAGGTCAAG GAACCAGCAG CACAAACACT
    GTTGGGGCAA CAGTGAACAG CCAAGCCGCC CAAGCTCAGC CTCCTGCCAT GACGTCCAGC
    AGGAAGGGCA CATTCACAGA TGACTTGCAC AAGTTGGTAG ACAATTGGGC CCGAGATGCC
    ATGAATCTCT CAGGCAGGAG AGGAAGCAAA GGGCACATGA ATTACGAGGG CCCTGGAATG
    GCAAGGAAGT TCTCTGCACC TGGGCAACTG TGCATCTCCA TGACCTCGAA CCTGGGTGGC
    TCTGCCCCCA TCTCTGCAGC ATCAGCTACC TCTCTAGGTC ACTTCACCAA GTCTATGTGC
    CCCCCACAGC AGTATGGCTT TCCAGCTACC CCATTTGGCG CTCAATGGAG TGGGACGGGT
    GGCCCAGCAC CACAGCCACT TGGCCAGTTC CAACCTGTGG GAACTGCCTC CTTGCAGAAT
    TTCAACATCA GCAATTTGCA GAAATCCATC AGCAACCCCC CAGGCTCCAA CCTGCGGACC
    ACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71618
    Clone ID Related Accession (Same CDS sequence) XM_011521002.2
    Accession Version XM_011521002.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7926bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase WNK1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521002.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG
    TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT
    CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT
    AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT
    GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT
    ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT
    ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA
    GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG
    GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA
    CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT
    CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG
    GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG
    AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG
    AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA
    GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT
    CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA
    GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG
    AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC
    CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC
    GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC
    TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA
    TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA
    ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGGGAAT ACAGCAGACA
    GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC
    ACTACTGCAC AGCCAGTGAG TCAGCCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT
    GGAAAACAGG GCTTCCCACC TCGACTGCCA CCACAGTACC CAGGAGATTC AAATATTGCT
    CCCTCTTCCA ACGTGGCTTC TGTTTGCATC CATTCTACAG TCCTATCCCC TCCCATGCCG
    ACAGAAGTAC TGGCTACACC TGGGTACTTT CCCACAGTGG TGCAGCCTTA TGTGGAATCA
    AATCTTTTAG TTCCTATGGG TGGTGTAGGA GGACAGGTTC AAGTGTCCCA GCCAGGAGGG
    AGTTTAGCAC AAGCCCCCAC TACATCCTCC CAGCAAGCAG TTTTGGAGAG TACTCAGGGA
    GTCTCTCAGG TTGCTCCTGC AGAGCCAGTT GCAGTAGCAC AGACCCAAGC TACCCAGCCG
    ACCACTTTGG CTTCCTCTGT AGACAGTGCA CATTCAGATG TTGCTTCAGG TATGAGTGAT
    GGCAATGAGA ACGTCCCATC TTCCAGTGGA AGGCATGAAG GAAGAACTAC AAAACGGCAT
    TACCGAAAAT CTGTAAGGAG TCGCTCTCGA CATGAAAAAA CTTCACGCCC AAAATTAAGA
    ATTTTGAATG TTTCAAATAA AGGAGACCGA GTAGTAGAAT GTCAATTAGA GACTCATAAT
    AGGAAAATGG TTACATTCAA ATTTGACCTA GATGGTGACA ACCCCGAGGA GATAGCAACA
    ATTATGGTGA ACAATGACTT TATTCTAGCA ATAGAGAGAG AGTCGTTTGT GGATCAAGTG
    CGAGAAATTA TTGAAAAAGC TGATGAAATG CTCAGTGAGG ATGTCAGTGT GGAACCAGAG
    GGTGATCAGG GATTGGAGAG TCTACAAGGA AAGGATGACT ATGGCTTTTC AGGTTCTCAG
    AAATTGGAAG GAGAGTTCAA ACAACCAATT CCTGCGTCTT CCATGCCACA GCAAATAGGC
    ATTCCTACCA GTTCTTTAAC TCAAGTTGTT CATTCTGCGG GAAGGCGGTT TATAGTGAGT
    CCTGTGCCAG AAAGCCGATT ACGAGAATCA AAAGTTTTCC CCAGTGAAAT AACAGATACA
    GTTGCTGCCT CTACAGCTCA GAGCCCTGGA ATGAACTTGT CTCACTCTGC ATCATCCCTT
    AGTCTACAAC AGGCCTTTTC TGAACTTAGA CGTGCCCAAA TGACAGAAGG ACCCAACACA
    GCACCTCCAA ACTTTAGTCA TACAGGACCA ACATTTCCAG TAGTACCTCC TTTCTTAAGT
    AGCATTGCTG GAGTCCCAAC CACAGCAGCA GCCACAGCAC CAGTCCCTGC AACAAGCAGC
    CCTCCTAATG ACATTTCCAC ATCAGTAATT CAGTCTGAGG TTACAGTGCC CACTGAAGAG
    GGGATTGCTG GAGTTGCCAC CAGCACAGGT GTGGTAACTT CAGGTGGTCT CCCCATACCA
    CCTGTGTCTG AATCACCAGT ACTTTCCAGC GTAGTTTCAA GTATCACAAT ACCTGCAGTT
    GTCTCAATAT CTACTACATC CCCGTCACTT CAAGTCCCCA CATCCACATC TGAGATCGTT
    GTTTCTAGTA CAGCACTGTA TCCTTCAGTA ACAGTTTCAG CAACTTCAGC CTCTGCAGGG
    GGCAGTACTG CTACCCCAGG TCCTAAGCCT CCAGCTGTAG TATCTCAGCA GGCAGCAGGC
    AGCACTACTG TGGGAGCCAC ATTAACATCA GTTTCTACCA CCACTTCATT CCCAAGCACA
    GCTTCACAGC TGTGCATTCA GCTTAGCAGC AGTACTTCTA CTCCTACTTT AGCTGAAACC
    GTGGTAGTTA GCGCACACTC ACTAGATAAG ACATCTCATA GCAGTACAAC TGGATTGGCT
    TTCTCCCTCT CTGCACCATC TTCCTCTTCC TCTCCTGGAG CAGGAGTGTC TAGTTATATT
    TCTCAGCCTG GTGGGCTGCA TCCTTTGGTC ATTCCATCAG TGATAGCTTC TACTCCTATT
    CTTCCCCAAG CAGCAGGACC TACTTCTACA CCTTTATTAC CCCAAGTACC TAGTATCCCA
    CCCTTGGTAC AGCCTGTTGC CAATGTGCCT GCTGTACAGC AGACACTAAT TCATAGTCAG
    CCTCAACCAG CTTTGCTTCC CAACCAGCCC CATACTCATT GTCCTGAAGT AGATTCTGAT
    ACACAACCCA AAGCTCCTGG AATTGATGAC ATAAAGACTC TAGAAGAAAA GCTGCGGTCT
    CTGTTCAGTG AACACAGCTC ATCTGGAGCT CAGCATGCCT CTGTCTCACT GGAGACCTCA
    CTAGTCATAG AGAGCACTGT CACACCAGGC ATCCCAACTA CTGCTGTTGC ACCAAGCAAA
    CTCCTGACTT CTACCACAAG TACTTGCTTA CCACCAACCA ATTTACCACT AGGAACAGTT
    GCTTTGCCAG TTACACCAGT GGTCACACCT GGGCAAGTTT CTACCCCAGT CAGCACTACT
    ACATCAGGAG TGAAACCTGG AACTGCTCCC TCCAAGCCAC CTCTAACTAA GGCTCCGGTG
    CTGCCAGTGG GTACTGAACT TCCAGCAGGT ACTCTACCCA GCGAGCAGCT GCCACCTTTT
    CCAGGACCTT CTCTAACCCA GTCCCAGCAA CCTCTAGAGG ATCTTGATGC TCAATTGAGA
    AGAACACTTA GTCCAGAGAT GATCACAGTG ACTTCTGCGG TTGGTCCTGT GTCCATGGCG
    GCTCCAACAG CAATCACAGA AGCAGGAACA CAGCCTCAGA AGGGTGTTTC TCAAGTCAAA
    GAAGGCCCTG TCCTAGCAAC TAGTTCAGGA GCTGGTGTTT TTAAGATGGG ACGATTTCAG
    GTTTCTGTTG CAGCAGACGG TGCCCAGAAA GAGGGTAAAA ATAAGTCAGA AGATGCAAAG
    TCTGTTCATT TTGAATCCAG CACCTCAGAG TCCTCAGTGC TATCAAGTAG TAGTCCAGAG
    AGTACCTTGG TGAAACCAGA GCCGAATGGC ATAACCATCC CTGGTATCTC TTCAGATGTG
    CCAGAGAGTG CCCACAAAAC TACTGCCTCA GAGGCAAAGT CAGACACTGG GCAGCCTACC
    AAGGTTGGAC GTTTTCAGGT GACAACTACA GCAAACAAAG TGGGTCGTTT CTCTGTATCA
    AAAACTGAGG ACAAGATCAC TGACACAAAG AAAGAAGGAC CAGTGGCATC TCCTCCTTTT
    ATGGATTTGG AACAAGCTGT TCTTCCTGCT GTGATACCAA AGAAAGAGAA GCCTGAACTG
    TCAGAGCCTT CACATCTAAA TGGGCCGTCT TCTGACCCGG AGGCCGCTTT TTTAAGTAGG
    GATGTGGATG ATGGTTCCGG TAGTCCACAC TCGCCCCATC AGCTGAGCTC AAAGAGCCTT
    CCTAGCCAGA ATCTAAGTCA AAGCCTTAGT AATTCATTTA ACTCCTCTTA CATGAGTAGC
    GACAATGAGT CAGATATCGA AGATGAAGAC TTAAAGTTAG AGCTGCGACG ACTACGAGAT
    AAACATCTCA AAGAGATTCA GGACCTGCAG AGTCGCCAGA AGCATGAAAT TGAATCTTTG
    TATACCAAAC TGGGCAAGGT GCCCCCTGCT GTTATTATTC CCCCAGCTGC TCCCCTTTCA
    GGGAGAAGAC GACGACCCAC TAAAAGCAAA GGCAGCAAAT CTAGTCGAAG CAGTTCCTTG
    GGGAATAAAA GCCCCCAGCT TTCAGGTAAC CTGTCTGGTC AGAGTGCAGC TTCAGTCTTG
    CACCCCCAGC AGACCCTCCA CCCTCCTGGC AACATCCCAG AGTCCGGGCA GAATCAGCTG
    TTACAGCCCC TTAAGCCATC TCCCTCCAGT GACAACCTCT ATTCAGCCTT CACCAGTGAT
    GGTGCCATTT CAGTACCAAG CCTTTCTGCT CCAGGTCAAG GAACCAGCAG CACAAACACT
    GTTGGGGCAA CAGTGAACAG CCAAGCCGCC CAAGCTCAGC CTCCTGCCAT GACGTCCAGC
    AGGAAGGGCA CATTCACAGA TGACTTGCAC AAGTTGGTAG ACAATTGGGC CCGAGATGCC
    ATGAATCTCT CAGGCAGGAG AGGAAGCAAA GGGCACATGA ATTACGAGGG CCCTGGAATG
    GCAAGGAAGT TCTCTGCACC TGGGCAACTG TGCATCTCCA TGACCTCGAA CCTGGGTGGC
    TCTGCCCCCA TCTCTGCAGC ATCAGCTACC TCTCTAGGTC ACTTCACCAA GTCTATGTGC
    CCCCCACAGC AGTATGGCTT TCCAGCTACC CCATTTGGCG CTCAATGGAG TGGGACGGGT
    GGCCCAGCAC CACAGCCACT TGGCCAGTTC CAACCTGTGG GAACTGCCTC CTTGCAGAAT
    TTCAACATCA GCAATTTGCA GAAATCCATC AGCAACCCCC CAGGCTCCAA CCTGCGGACC
    ACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011519304.1
    CDS969..8894
    Translation

    Target ORF information:

    RefSeq Version XM_011521002.2
    Organism Homo sapiens(human)
    Definition Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011521002.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG
    TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT
    CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT
    AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT
    GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT
    ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT
    ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA
    GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG
    GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA
    CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT
    CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG
    GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG
    AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG
    AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA
    GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT
    CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA
    GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG
    AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC
    CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC
    GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC
    TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA
    TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA
    ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGGGAAT ACAGCAGACA
    GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC
    ACTACTGCAC AGCCAGTGAG TCAGCCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT
    GGAAAACAGG GCTTCCCACC TCGACTGCCA CCACAGTACC CAGGAGATTC AAATATTGCT
    CCCTCTTCCA ACGTGGCTTC TGTTTGCATC CATTCTACAG TCCTATCCCC TCCCATGCCG
    ACAGAAGTAC TGGCTACACC TGGGTACTTT CCCACAGTGG TGCAGCCTTA TGTGGAATCA
    AATCTTTTAG TTCCTATGGG TGGTGTAGGA GGACAGGTTC AAGTGTCCCA GCCAGGAGGG
    AGTTTAGCAC AAGCCCCCAC TACATCCTCC CAGCAAGCAG TTTTGGAGAG TACTCAGGGA
    GTCTCTCAGG TTGCTCCTGC AGAGCCAGTT GCAGTAGCAC AGACCCAAGC TACCCAGCCG
    ACCACTTTGG CTTCCTCTGT AGACAGTGCA CATTCAGATG TTGCTTCAGG TATGAGTGAT
    GGCAATGAGA ACGTCCCATC TTCCAGTGGA AGGCATGAAG GAAGAACTAC AAAACGGCAT
    TACCGAAAAT CTGTAAGGAG TCGCTCTCGA CATGAAAAAA CTTCACGCCC AAAATTAAGA
    ATTTTGAATG TTTCAAATAA AGGAGACCGA GTAGTAGAAT GTCAATTAGA GACTCATAAT
    AGGAAAATGG TTACATTCAA ATTTGACCTA GATGGTGACA ACCCCGAGGA GATAGCAACA
    ATTATGGTGA ACAATGACTT TATTCTAGCA ATAGAGAGAG AGTCGTTTGT GGATCAAGTG
    CGAGAAATTA TTGAAAAAGC TGATGAAATG CTCAGTGAGG ATGTCAGTGT GGAACCAGAG
    GGTGATCAGG GATTGGAGAG TCTACAAGGA AAGGATGACT ATGGCTTTTC AGGTTCTCAG
    AAATTGGAAG GAGAGTTCAA ACAACCAATT CCTGCGTCTT CCATGCCACA GCAAATAGGC
    ATTCCTACCA GTTCTTTAAC TCAAGTTGTT CATTCTGCGG GAAGGCGGTT TATAGTGAGT
    CCTGTGCCAG AAAGCCGATT ACGAGAATCA AAAGTTTTCC CCAGTGAAAT AACAGATACA
    GTTGCTGCCT CTACAGCTCA GAGCCCTGGA ATGAACTTGT CTCACTCTGC ATCATCCCTT
    AGTCTACAAC AGGCCTTTTC TGAACTTAGA CGTGCCCAAA TGACAGAAGG ACCCAACACA
    GCACCTCCAA ACTTTAGTCA TACAGGACCA ACATTTCCAG TAGTACCTCC TTTCTTAAGT
    AGCATTGCTG GAGTCCCAAC CACAGCAGCA GCCACAGCAC CAGTCCCTGC AACAAGCAGC
    CCTCCTAATG ACATTTCCAC ATCAGTAATT CAGTCTGAGG TTACAGTGCC CACTGAAGAG
    GGGATTGCTG GAGTTGCCAC CAGCACAGGT GTGGTAACTT CAGGTGGTCT CCCCATACCA
    CCTGTGTCTG AATCACCAGT ACTTTCCAGC GTAGTTTCAA GTATCACAAT ACCTGCAGTT
    GTCTCAATAT CTACTACATC CCCGTCACTT CAAGTCCCCA CATCCACATC TGAGATCGTT
    GTTTCTAGTA CAGCACTGTA TCCTTCAGTA ACAGTTTCAG CAACTTCAGC CTCTGCAGGG
    GGCAGTACTG CTACCCCAGG TCCTAAGCCT CCAGCTGTAG TATCTCAGCA GGCAGCAGGC
    AGCACTACTG TGGGAGCCAC ATTAACATCA GTTTCTACCA CCACTTCATT CCCAAGCACA
    GCTTCACAGC TGTGCATTCA GCTTAGCAGC AGTACTTCTA CTCCTACTTT AGCTGAAACC
    GTGGTAGTTA GCGCACACTC ACTAGATAAG ACATCTCATA GCAGTACAAC TGGATTGGCT
    TTCTCCCTCT CTGCACCATC TTCCTCTTCC TCTCCTGGAG CAGGAGTGTC TAGTTATATT
    TCTCAGCCTG GTGGGCTGCA TCCTTTGGTC ATTCCATCAG TGATAGCTTC TACTCCTATT
    CTTCCCCAAG CAGCAGGACC TACTTCTACA CCTTTATTAC CCCAAGTACC TAGTATCCCA
    CCCTTGGTAC AGCCTGTTGC CAATGTGCCT GCTGTACAGC AGACACTAAT TCATAGTCAG
    CCTCAACCAG CTTTGCTTCC CAACCAGCCC CATACTCATT GTCCTGAAGT AGATTCTGAT
    ACACAACCCA AAGCTCCTGG AATTGATGAC ATAAAGACTC TAGAAGAAAA GCTGCGGTCT
    CTGTTCAGTG AACACAGCTC ATCTGGAGCT CAGCATGCCT CTGTCTCACT GGAGACCTCA
    CTAGTCATAG AGAGCACTGT CACACCAGGC ATCCCAACTA CTGCTGTTGC ACCAAGCAAA
    CTCCTGACTT CTACCACAAG TACTTGCTTA CCACCAACCA ATTTACCACT AGGAACAGTT
    GCTTTGCCAG TTACACCAGT GGTCACACCT GGGCAAGTTT CTACCCCAGT CAGCACTACT
    ACATCAGGAG TGAAACCTGG AACTGCTCCC TCCAAGCCAC CTCTAACTAA GGCTCCGGTG
    CTGCCAGTGG GTACTGAACT TCCAGCAGGT ACTCTACCCA GCGAGCAGCT GCCACCTTTT
    CCAGGACCTT CTCTAACCCA GTCCCAGCAA CCTCTAGAGG ATCTTGATGC TCAATTGAGA
    AGAACACTTA GTCCAGAGAT GATCACAGTG ACTTCTGCGG TTGGTCCTGT GTCCATGGCG
    GCTCCAACAG CAATCACAGA AGCAGGAACA CAGCCTCAGA AGGGTGTTTC TCAAGTCAAA
    GAAGGCCCTG TCCTAGCAAC TAGTTCAGGA GCTGGTGTTT TTAAGATGGG ACGATTTCAG
    GTTTCTGTTG CAGCAGACGG TGCCCAGAAA GAGGGTAAAA ATAAGTCAGA AGATGCAAAG
    TCTGTTCATT TTGAATCCAG CACCTCAGAG TCCTCAGTGC TATCAAGTAG TAGTCCAGAG
    AGTACCTTGG TGAAACCAGA GCCGAATGGC ATAACCATCC CTGGTATCTC TTCAGATGTG
    CCAGAGAGTG CCCACAAAAC TACTGCCTCA GAGGCAAAGT CAGACACTGG GCAGCCTACC
    AAGGTTGGAC GTTTTCAGGT GACAACTACA GCAAACAAAG TGGGTCGTTT CTCTGTATCA
    AAAACTGAGG ACAAGATCAC TGACACAAAG AAAGAAGGAC CAGTGGCATC TCCTCCTTTT
    ATGGATTTGG AACAAGCTGT TCTTCCTGCT GTGATACCAA AGAAAGAGAA GCCTGAACTG
    TCAGAGCCTT CACATCTAAA TGGGCCGTCT TCTGACCCGG AGGCCGCTTT TTTAAGTAGG
    GATGTGGATG ATGGTTCCGG TAGTCCACAC TCGCCCCATC AGCTGAGCTC AAAGAGCCTT
    CCTAGCCAGA ATCTAAGTCA AAGCCTTAGT AATTCATTTA ACTCCTCTTA CATGAGTAGC
    GACAATGAGT CAGATATCGA AGATGAAGAC TTAAAGTTAG AGCTGCGACG ACTACGAGAT
    AAACATCTCA AAGAGATTCA GGACCTGCAG AGTCGCCAGA AGCATGAAAT TGAATCTTTG
    TATACCAAAC TGGGCAAGGT GCCCCCTGCT GTTATTATTC CCCCAGCTGC TCCCCTTTCA
    GGGAGAAGAC GACGACCCAC TAAAAGCAAA GGCAGCAAAT CTAGTCGAAG CAGTTCCTTG
    GGGAATAAAA GCCCCCAGCT TTCAGGTAAC CTGTCTGGTC AGAGTGCAGC TTCAGTCTTG
    CACCCCCAGC AGACCCTCCA CCCTCCTGGC AACATCCCAG AGTCCGGGCA GAATCAGCTG
    TTACAGCCCC TTAAGCCATC TCCCTCCAGT GACAACCTCT ATTCAGCCTT CACCAGTGAT
    GGTGCCATTT CAGTACCAAG CCTTTCTGCT CCAGGTCAAG GAACCAGCAG CACAAACACT
    GTTGGGGCAA CAGTGAACAG CCAAGCCGCC CAAGCTCAGC CTCCTGCCAT GACGTCCAGC
    AGGAAGGGCA CATTCACAGA TGACTTGCAC AAGTTGGTAG ACAATTGGGC CCGAGATGCC
    ATGAATCTCT CAGGCAGGAG AGGAAGCAAA GGGCACATGA ATTACGAGGG CCCTGGAATG
    GCAAGGAAGT TCTCTGCACC TGGGCAACTG TGCATCTCCA TGACCTCGAA CCTGGGTGGC
    TCTGCCCCCA TCTCTGCAGC ATCAGCTACC TCTCTAGGTC ACTTCACCAA GTCTATGTGC
    CCCCCACAGC AGTATGGCTT TCCAGCTACC CCATTTGGCG CTCAATGGAG TGGGACGGGT
    GGCCCAGCAC CACAGCCACT TGGCCAGTTC CAACCTGTGG GAACTGCCTC CTTGCAGAAT
    TTCAACATCA GCAATTTGCA GAAATCCATC AGCAACCCCC CAGGCTCCAA CCTGCGGACC
    ACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71619
    Clone ID Related Accession (Same CDS sequence) XM_011521003.2
    Accession Version XM_011521003.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7650bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase WNK1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521003.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG
    TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT
    CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT
    AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT
    GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT
    ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT
    ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA
    GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG
    GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA
    CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT
    CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG
    GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG
    AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG
    AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA
    GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT
    CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA
    GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG
    AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC
    CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC
    GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC
    TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA
    TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA
    ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGCAGGG AATACAGCAG
    ACAGCCCCTC CTCAACAGAC AGTGCAGTAT TCACTTTCAC AGACATCAAC CTCCAGTGAG
    GCCACTACTG CACAGCCAGT GAGTCAGCCT CAAGCTCCAC AAGTCTTGCC TCAAGTATCA
    GCTGGAAAAC AGAGTACTCA GGGAGTCTCT CAGGTTGCTC CTGCAGAGCC AGTTGCAGTA
    GCACAGACCC AAGCTACCCA GCCGACCACT TTGGCTTCCT CTGTAGACAG TGCACATTCA
    GATGTTGCTT CAGGTATGAG TGATGGCAAT GAGAACGTCC CATCTTCCAG TGGAAGGCAT
    GAAGGAAGAA CTACAAAACG GCATTACCGA AAATCTGTAA GGAGTCGCTC TCGACATGAA
    AAAACTTCAC GCCCAAAATT AAGAATTTTG AATGTTTCAA ATAAAGGAGA CCGAGTAGTA
    GAATGTCAAT TAGAGACTCA TAATAGGAAA ATGGTTACAT TCAAATTTGA CCTAGATGGT
    GACAACCCCG AGGAGATAGC AACAATTATG GTGAACAATG ACTTTATTCT AGCAATAGAG
    AGAGAGTCGT TTGTGGATCA AGTGCGAGAA ATTATTGAAA AAGCTGATGA AATGCTCAGT
    GAGGATGTCA GTGTGGAACC AGAGGGTGAT CAGGGATTGG AGAGTCTACA AGGAAAGGAT
    GACTATGGCT TTTCAGGTTC TCAGAAATTG GAAGGAGAGT TCAAACAACC AATTCCTGCG
    TCTTCCATGC CACAGCAAAT AGGCATTCCT ACCAGTTCTT TAACTCAAGT TGTTCATTCT
    GCGGGAAGGC GGTTTATAGT GAGTCCTGTG CCAGAAAGCC GATTACGAGA ATCAAAAGTT
    TTCCCCAGTG AAATAACAGA TACAGTTGCT GCCTCTACAG CTCAGAGCCC TGGAATGAAC
    TTGTCTCACT CTGCATCATC CCTTAGTCTA CAACAGGCCT TTTCTGAACT TAGACGTGCC
    CAAATGACAG AAGGACCCAA CACAGCACCT CCAAACTTTA GTCATACAGG ACCAACATTT
    CCAGTAGTAC CTCCTTTCTT AAGTAGCATT GCTGGAGTCC CAACCACAGC AGCAGCCACA
    GCACCAGTCC CTGCAACAAG CAGCCCTCCT AATGACATTT CCACATCAGT AATTCAGTCT
    GAGGTTACAG TGCCCACTGA AGAGGGGATT GCTGGAGTTG CCACCAGCAC AGGTGTGGTA
    ACTTCAGGTG GTCTCCCCAT ACCACCTGTG TCTGAATCAC CAGTACTTTC CAGCGTAGTT
    TCAAGTATCA CAATACCTGC AGTTGTCTCA ATATCTACTA CATCCCCGTC ACTTCAAGTC
    CCCACATCCA CATCTGAGAT CGTTGTTTCT AGTACAGCAC TGTATCCTTC AGTAACAGTT
    TCAGCAACTT CAGCCTCTGC AGGGGGCAGT ACTGCTACCC CAGGTCCTAA GCCTCCAGCT
    GTAGTATCTC AGCAGGCAGC AGGCAGCACT ACTGTGGGAG CCACATTAAC ATCAGTTTCT
    ACCACCACTT CATTCCCAAG CACAGCTTCA CAGCTGTGCA TTCAGCTTAG CAGCAGTACT
    TCTACTCCTA CTTTAGCTGA AACCGTGGTA GTTAGCGCAC ACTCACTAGA TAAGACATCT
    CATAGCAGTA CAACTGGATT GGCTTTCTCC CTCTCTGCAC CATCTTCCTC TTCCTCTCCT
    GGAGCAGGAG TGTCTAGTTA TATTTCTCAG CCTGGTGGGC TGCATCCTTT GGTCATTCCA
    TCAGTGATAG CTTCTACTCC TATTCTTCCC CAAGCAGCAG GACCTACTTC TACACCTTTA
    TTACCCCAAG TACCTAGTAT CCCACCCTTG GTACAGCCTG TTGCCAATGT GCCTGCTGTA
    CAGCAGACAC TAATTCATAG TCAGCCTCAA CCAGCTTTGC TTCCCAACCA GCCCCATACT
    CATTGTCCTG AAGTAGATTC TGATACACAA CCCAAAGCTC CTGGAATTGA TGACATAAAG
    ACTCTAGAAG AAAAGCTGCG GTCTCTGTTC AGTGAACACA GCTCATCTGG AGCTCAGCAT
    GCCTCTGTCT CACTGGAGAC CTCACTAGTC ATAGAGAGCA CTGTCACACC AGGCATCCCA
    ACTACTGCTG TTGCACCAAG CAAACTCCTG ACTTCTACCA CAAGTACTTG CTTACCACCA
    ACCAATTTAC CACTAGGAAC AGTTGCTTTG CCAGTTACAC CAGTGGTCAC ACCTGGGCAA
    GTTTCTACCC CAGTCAGCAC TACTACATCA GGAGTGAAAC CTGGAACTGC TCCCTCCAAG
    CCACCTCTAA CTAAGGCTCC GGTGCTGCCA GTGGGTACTG AACTTCCAGC AGGTACTCTA
    CCCAGCGAGC AGCTGCCACC TTTTCCAGGA CCTTCTCTAA CCCAGTCCCA GCAACCTCTA
    GAGGATCTTG ATGCTCAATT GAGAAGAACA CTTAGTCCAG AGATGATCAC AGTGACTTCT
    GCGGTTGGTC CTGTGTCCAT GGCGGCTCCA ACAGCAATCA CAGAAGCAGG AACACAGCCT
    CAGAAGGGTG TTTCTCAAGT CAAAGAAGGC CCTGTCCTAG CAACTAGTTC AGGAGCTGGT
    GTTTTTAAGA TGGGACGATT TCAGGTTTCT GTTGCAGCAG ACGGTGCCCA GAAAGAGGGT
    AAAAATAAGT CAGAAGATGC AAAGTCTGTT CATTTTGAAT CCAGCACCTC AGAGTCCTCA
    GTGCTATCAA GTAGTAGTCC AGAGAGTACC TTGGTGAAAC CAGAGCCGAA TGGCATAACC
    ATCCCTGGTA TCTCTTCAGA TGTGCCAGAG AGTGCCCACA AAACTACTGC CTCAGAGGCA
    AAGTCAGACA CTGGGCAGCC TACCAAGGTT GGACGTTTTC AGGTGACAAC TACAGCAAAC
    AAAGTGGGTC GTTTCTCTGT ATCAAAAACT GAGGACAAGA TCACTGACAC AAAGAAAGAA
    GGACCAGTGG CATCTCCTCC TTTTATGGAT TTGGAACAAG CTGTTCTTCC TGCTGTGATA
    CCAAAGAAAG AGAAGCCTGA ACTGTCAGAG CCTTCACATC TAAATGGGCC GTCTTCTGAC
    CCGGAGGCCG CTTTTTTAAG TAGGGATGTG GATGATGGTT CCGGTAGTCC ACACTCGCCC
    CATCAGCTGA GCTCAAAGAG CCTTCCTAGC CAGAATCTAA GTCAAAGCCT TAGTAATTCA
    TTTAACTCCT CTTACATGAG TAGCGACAAT GAGTCAGATA TCGAAGATGA AGACTTAAAG
    TTAGAGCTGC GACGACTACG AGATAAACAT CTCAAAGAGA TTCAGGACCT GCAGAGTCGC
    CAGAAGCATG AAATTGAATC TTTGTATACC AAACTGGGCA AGGTGCCCCC TGCTGTTATT
    ATTCCCCCAG CTGCTCCCCT TTCAGGGAGA AGACGACGAC CCACTAAAAG CAAAGGCAGC
    AAATCTAGTC GAAGCAGTTC CTTGGGGAAT AAAAGCCCCC AGCTTTCAGG TAACCTGTCT
    GGTCAGAGTG CAGCTTCAGT CTTGCACCCC CAGCAGACCC TCCACCCTCC TGGCAACATC
    CCAGAGTCCG GGCAGAATCA GCTGTTACAG CCCCTTAAGC CATCTCCCTC CAGTGACAAC
    CTCTATTCAG CCTTCACCAG TGATGGTGCC ATTTCAGTAC CAAGCCTTTC TGCTCCAGGT
    CAAGGAACCA GCAGCACAAA CACTGTTGGG GCAACAGTGA ACAGCCAAGC CGCCCAAGCT
    CAGCCTCCTG CCATGACGTC CAGCAGGAAG GGCACATTCA CAGATGACTT GCACAAGTTG
    GTAGACAATT GGGCCCGAGA TGCCATGAAT CTCTCAGGCA GGAGAGGAAG CAAAGGGCAC
    ATGAATTACG AGGGCCCTGG AATGGCAAGG AAGTTCTCTG CACCTGGGCA ACTGTGCATC
    TCCATGACCT CGAACCTGGG TGGCTCTGCC CCCATCTCTG CAGCATCAGC TACCTCTCTA
    GGTCACTTCA CCAAGTCTAT GTGCCCCCCA CAGCAGTATG GCTTTCCAGC TACCCCATTT
    GGCGCTCAAT GGAGTGGGAC GGGTGGCCCA GCACCACAGC CACTTGGCCA GTTCCAACCT
    GTGGGAACTG CCTCCTTGCA GAATTTCAAC ATCAGCAATT TGCAGAAATC CATCAGCAAC
    CCCCCAGGCT CCAACCTGCG GACCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011519305.1
    CDS969..8618
    Translation

    Target ORF information:

    RefSeq Version XM_011521003.2
    Organism Homo sapiens(human)
    Definition Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011521003.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG
    TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT
    CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT
    AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT
    GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT
    ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT
    ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA
    GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG
    GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA
    CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT
    CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG
    GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG
    AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG
    AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA
    GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT
    CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA
    GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG
    AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC
    CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC
    GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC
    TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA
    TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA
    ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGCAGGG AATACAGCAG
    ACAGCCCCTC CTCAACAGAC AGTGCAGTAT TCACTTTCAC AGACATCAAC CTCCAGTGAG
    GCCACTACTG CACAGCCAGT GAGTCAGCCT CAAGCTCCAC AAGTCTTGCC TCAAGTATCA
    GCTGGAAAAC AGAGTACTCA GGGAGTCTCT CAGGTTGCTC CTGCAGAGCC AGTTGCAGTA
    GCACAGACCC AAGCTACCCA GCCGACCACT TTGGCTTCCT CTGTAGACAG TGCACATTCA
    GATGTTGCTT CAGGTATGAG TGATGGCAAT GAGAACGTCC CATCTTCCAG TGGAAGGCAT
    GAAGGAAGAA CTACAAAACG GCATTACCGA AAATCTGTAA GGAGTCGCTC TCGACATGAA
    AAAACTTCAC GCCCAAAATT AAGAATTTTG AATGTTTCAA ATAAAGGAGA CCGAGTAGTA
    GAATGTCAAT TAGAGACTCA TAATAGGAAA ATGGTTACAT TCAAATTTGA CCTAGATGGT
    GACAACCCCG AGGAGATAGC AACAATTATG GTGAACAATG ACTTTATTCT AGCAATAGAG
    AGAGAGTCGT TTGTGGATCA AGTGCGAGAA ATTATTGAAA AAGCTGATGA AATGCTCAGT
    GAGGATGTCA GTGTGGAACC AGAGGGTGAT CAGGGATTGG AGAGTCTACA AGGAAAGGAT
    GACTATGGCT TTTCAGGTTC TCAGAAATTG GAAGGAGAGT TCAAACAACC AATTCCTGCG
    TCTTCCATGC CACAGCAAAT AGGCATTCCT ACCAGTTCTT TAACTCAAGT TGTTCATTCT
    GCGGGAAGGC GGTTTATAGT GAGTCCTGTG CCAGAAAGCC GATTACGAGA ATCAAAAGTT
    TTCCCCAGTG AAATAACAGA TACAGTTGCT GCCTCTACAG CTCAGAGCCC TGGAATGAAC
    TTGTCTCACT CTGCATCATC CCTTAGTCTA CAACAGGCCT TTTCTGAACT TAGACGTGCC
    CAAATGACAG AAGGACCCAA CACAGCACCT CCAAACTTTA GTCATACAGG ACCAACATTT
    CCAGTAGTAC CTCCTTTCTT AAGTAGCATT GCTGGAGTCC CAACCACAGC AGCAGCCACA
    GCACCAGTCC CTGCAACAAG CAGCCCTCCT AATGACATTT CCACATCAGT AATTCAGTCT
    GAGGTTACAG TGCCCACTGA AGAGGGGATT GCTGGAGTTG CCACCAGCAC AGGTGTGGTA
    ACTTCAGGTG GTCTCCCCAT ACCACCTGTG TCTGAATCAC CAGTACTTTC CAGCGTAGTT
    TCAAGTATCA CAATACCTGC AGTTGTCTCA ATATCTACTA CATCCCCGTC ACTTCAAGTC
    CCCACATCCA CATCTGAGAT CGTTGTTTCT AGTACAGCAC TGTATCCTTC AGTAACAGTT
    TCAGCAACTT CAGCCTCTGC AGGGGGCAGT ACTGCTACCC CAGGTCCTAA GCCTCCAGCT
    GTAGTATCTC AGCAGGCAGC AGGCAGCACT ACTGTGGGAG CCACATTAAC ATCAGTTTCT
    ACCACCACTT CATTCCCAAG CACAGCTTCA CAGCTGTGCA TTCAGCTTAG CAGCAGTACT
    TCTACTCCTA CTTTAGCTGA AACCGTGGTA GTTAGCGCAC ACTCACTAGA TAAGACATCT
    CATAGCAGTA CAACTGGATT GGCTTTCTCC CTCTCTGCAC CATCTTCCTC TTCCTCTCCT
    GGAGCAGGAG TGTCTAGTTA TATTTCTCAG CCTGGTGGGC TGCATCCTTT GGTCATTCCA
    TCAGTGATAG CTTCTACTCC TATTCTTCCC CAAGCAGCAG GACCTACTTC TACACCTTTA
    TTACCCCAAG TACCTAGTAT CCCACCCTTG GTACAGCCTG TTGCCAATGT GCCTGCTGTA
    CAGCAGACAC TAATTCATAG TCAGCCTCAA CCAGCTTTGC TTCCCAACCA GCCCCATACT
    CATTGTCCTG AAGTAGATTC TGATACACAA CCCAAAGCTC CTGGAATTGA TGACATAAAG
    ACTCTAGAAG AAAAGCTGCG GTCTCTGTTC AGTGAACACA GCTCATCTGG AGCTCAGCAT
    GCCTCTGTCT CACTGGAGAC CTCACTAGTC ATAGAGAGCA CTGTCACACC AGGCATCCCA
    ACTACTGCTG TTGCACCAAG CAAACTCCTG ACTTCTACCA CAAGTACTTG CTTACCACCA
    ACCAATTTAC CACTAGGAAC AGTTGCTTTG CCAGTTACAC CAGTGGTCAC ACCTGGGCAA
    GTTTCTACCC CAGTCAGCAC TACTACATCA GGAGTGAAAC CTGGAACTGC TCCCTCCAAG
    CCACCTCTAA CTAAGGCTCC GGTGCTGCCA GTGGGTACTG AACTTCCAGC AGGTACTCTA
    CCCAGCGAGC AGCTGCCACC TTTTCCAGGA CCTTCTCTAA CCCAGTCCCA GCAACCTCTA
    GAGGATCTTG ATGCTCAATT GAGAAGAACA CTTAGTCCAG AGATGATCAC AGTGACTTCT
    GCGGTTGGTC CTGTGTCCAT GGCGGCTCCA ACAGCAATCA CAGAAGCAGG AACACAGCCT
    CAGAAGGGTG TTTCTCAAGT CAAAGAAGGC CCTGTCCTAG CAACTAGTTC AGGAGCTGGT
    GTTTTTAAGA TGGGACGATT TCAGGTTTCT GTTGCAGCAG ACGGTGCCCA GAAAGAGGGT
    AAAAATAAGT CAGAAGATGC AAAGTCTGTT CATTTTGAAT CCAGCACCTC AGAGTCCTCA
    GTGCTATCAA GTAGTAGTCC AGAGAGTACC TTGGTGAAAC CAGAGCCGAA TGGCATAACC
    ATCCCTGGTA TCTCTTCAGA TGTGCCAGAG AGTGCCCACA AAACTACTGC CTCAGAGGCA
    AAGTCAGACA CTGGGCAGCC TACCAAGGTT GGACGTTTTC AGGTGACAAC TACAGCAAAC
    AAAGTGGGTC GTTTCTCTGT ATCAAAAACT GAGGACAAGA TCACTGACAC AAAGAAAGAA
    GGACCAGTGG CATCTCCTCC TTTTATGGAT TTGGAACAAG CTGTTCTTCC TGCTGTGATA
    CCAAAGAAAG AGAAGCCTGA ACTGTCAGAG CCTTCACATC TAAATGGGCC GTCTTCTGAC
    CCGGAGGCCG CTTTTTTAAG TAGGGATGTG GATGATGGTT CCGGTAGTCC ACACTCGCCC
    CATCAGCTGA GCTCAAAGAG CCTTCCTAGC CAGAATCTAA GTCAAAGCCT TAGTAATTCA
    TTTAACTCCT CTTACATGAG TAGCGACAAT GAGTCAGATA TCGAAGATGA AGACTTAAAG
    TTAGAGCTGC GACGACTACG AGATAAACAT CTCAAAGAGA TTCAGGACCT GCAGAGTCGC
    CAGAAGCATG AAATTGAATC TTTGTATACC AAACTGGGCA AGGTGCCCCC TGCTGTTATT
    ATTCCCCCAG CTGCTCCCCT TTCAGGGAGA AGACGACGAC CCACTAAAAG CAAAGGCAGC
    AAATCTAGTC GAAGCAGTTC CTTGGGGAAT AAAAGCCCCC AGCTTTCAGG TAACCTGTCT
    GGTCAGAGTG CAGCTTCAGT CTTGCACCCC CAGCAGACCC TCCACCCTCC TGGCAACATC
    CCAGAGTCCG GGCAGAATCA GCTGTTACAG CCCCTTAAGC CATCTCCCTC CAGTGACAAC
    CTCTATTCAG CCTTCACCAG TGATGGTGCC ATTTCAGTAC CAAGCCTTTC TGCTCCAGGT
    CAAGGAACCA GCAGCACAAA CACTGTTGGG GCAACAGTGA ACAGCCAAGC CGCCCAAGCT
    CAGCCTCCTG CCATGACGTC CAGCAGGAAG GGCACATTCA CAGATGACTT GCACAAGTTG
    GTAGACAATT GGGCCCGAGA TGCCATGAAT CTCTCAGGCA GGAGAGGAAG CAAAGGGCAC
    ATGAATTACG AGGGCCCTGG AATGGCAAGG AAGTTCTCTG CACCTGGGCA ACTGTGCATC
    TCCATGACCT CGAACCTGGG TGGCTCTGCC CCCATCTCTG CAGCATCAGC TACCTCTCTA
    GGTCACTTCA CCAAGTCTAT GTGCCCCCCA CAGCAGTATG GCTTTCCAGC TACCCCATTT
    GGCGCTCAAT GGAGTGGGAC GGGTGGCCCA GCACCACAGC CACTTGGCCA GTTCCAACCT
    GTGGGAACTG CCTCCTTGCA GAATTTCAAC ATCAGCAATT TGCAGAAATC CATCAGCAAC
    CCCCCAGGCT CCAACCTGCG GACCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71620
    Clone ID Related Accession (Same CDS sequence) XM_011521004.2
    Accession Version XM_011521004.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7647bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase WNK1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521004.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG
    TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT
    CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT
    AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT
    GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT
    ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT
    ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA
    GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG
    GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA
    CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT
    CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG
    GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG
    AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG
    AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA
    GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT
    CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA
    GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG
    AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC
    CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC
    GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC
    TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA
    TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA
    ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGGGAAT ACAGCAGACA
    GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC
    ACTACTGCAC AGCCAGTGAG TCAGCCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT
    GGAAAACAGA GTACTCAGGG AGTCTCTCAG GTTGCTCCTG CAGAGCCAGT TGCAGTAGCA
    CAGACCCAAG CTACCCAGCC GACCACTTTG GCTTCCTCTG TAGACAGTGC ACATTCAGAT
    GTTGCTTCAG GTATGAGTGA TGGCAATGAG AACGTCCCAT CTTCCAGTGG AAGGCATGAA
    GGAAGAACTA CAAAACGGCA TTACCGAAAA TCTGTAAGGA GTCGCTCTCG ACATGAAAAA
    ACTTCACGCC CAAAATTAAG AATTTTGAAT GTTTCAAATA AAGGAGACCG AGTAGTAGAA
    TGTCAATTAG AGACTCATAA TAGGAAAATG GTTACATTCA AATTTGACCT AGATGGTGAC
    AACCCCGAGG AGATAGCAAC AATTATGGTG AACAATGACT TTATTCTAGC AATAGAGAGA
    GAGTCGTTTG TGGATCAAGT GCGAGAAATT ATTGAAAAAG CTGATGAAAT GCTCAGTGAG
    GATGTCAGTG TGGAACCAGA GGGTGATCAG GGATTGGAGA GTCTACAAGG AAAGGATGAC
    TATGGCTTTT CAGGTTCTCA GAAATTGGAA GGAGAGTTCA AACAACCAAT TCCTGCGTCT
    TCCATGCCAC AGCAAATAGG CATTCCTACC AGTTCTTTAA CTCAAGTTGT TCATTCTGCG
    GGAAGGCGGT TTATAGTGAG TCCTGTGCCA GAAAGCCGAT TACGAGAATC AAAAGTTTTC
    CCCAGTGAAA TAACAGATAC AGTTGCTGCC TCTACAGCTC AGAGCCCTGG AATGAACTTG
    TCTCACTCTG CATCATCCCT TAGTCTACAA CAGGCCTTTT CTGAACTTAG ACGTGCCCAA
    ATGACAGAAG GACCCAACAC AGCACCTCCA AACTTTAGTC ATACAGGACC AACATTTCCA
    GTAGTACCTC CTTTCTTAAG TAGCATTGCT GGAGTCCCAA CCACAGCAGC AGCCACAGCA
    CCAGTCCCTG CAACAAGCAG CCCTCCTAAT GACATTTCCA CATCAGTAAT TCAGTCTGAG
    GTTACAGTGC CCACTGAAGA GGGGATTGCT GGAGTTGCCA CCAGCACAGG TGTGGTAACT
    TCAGGTGGTC TCCCCATACC ACCTGTGTCT GAATCACCAG TACTTTCCAG CGTAGTTTCA
    AGTATCACAA TACCTGCAGT TGTCTCAATA TCTACTACAT CCCCGTCACT TCAAGTCCCC
    ACATCCACAT CTGAGATCGT TGTTTCTAGT ACAGCACTGT ATCCTTCAGT AACAGTTTCA
    GCAACTTCAG CCTCTGCAGG GGGCAGTACT GCTACCCCAG GTCCTAAGCC TCCAGCTGTA
    GTATCTCAGC AGGCAGCAGG CAGCACTACT GTGGGAGCCA CATTAACATC AGTTTCTACC
    ACCACTTCAT TCCCAAGCAC AGCTTCACAG CTGTGCATTC AGCTTAGCAG CAGTACTTCT
    ACTCCTACTT TAGCTGAAAC CGTGGTAGTT AGCGCACACT CACTAGATAA GACATCTCAT
    AGCAGTACAA CTGGATTGGC TTTCTCCCTC TCTGCACCAT CTTCCTCTTC CTCTCCTGGA
    GCAGGAGTGT CTAGTTATAT TTCTCAGCCT GGTGGGCTGC ATCCTTTGGT CATTCCATCA
    GTGATAGCTT CTACTCCTAT TCTTCCCCAA GCAGCAGGAC CTACTTCTAC ACCTTTATTA
    CCCCAAGTAC CTAGTATCCC ACCCTTGGTA CAGCCTGTTG CCAATGTGCC TGCTGTACAG
    CAGACACTAA TTCATAGTCA GCCTCAACCA GCTTTGCTTC CCAACCAGCC CCATACTCAT
    TGTCCTGAAG TAGATTCTGA TACACAACCC AAAGCTCCTG GAATTGATGA CATAAAGACT
    CTAGAAGAAA AGCTGCGGTC TCTGTTCAGT GAACACAGCT CATCTGGAGC TCAGCATGCC
    TCTGTCTCAC TGGAGACCTC ACTAGTCATA GAGAGCACTG TCACACCAGG CATCCCAACT
    ACTGCTGTTG CACCAAGCAA ACTCCTGACT TCTACCACAA GTACTTGCTT ACCACCAACC
    AATTTACCAC TAGGAACAGT TGCTTTGCCA GTTACACCAG TGGTCACACC TGGGCAAGTT
    TCTACCCCAG TCAGCACTAC TACATCAGGA GTGAAACCTG GAACTGCTCC CTCCAAGCCA
    CCTCTAACTA AGGCTCCGGT GCTGCCAGTG GGTACTGAAC TTCCAGCAGG TACTCTACCC
    AGCGAGCAGC TGCCACCTTT TCCAGGACCT TCTCTAACCC AGTCCCAGCA ACCTCTAGAG
    GATCTTGATG CTCAATTGAG AAGAACACTT AGTCCAGAGA TGATCACAGT GACTTCTGCG
    GTTGGTCCTG TGTCCATGGC GGCTCCAACA GCAATCACAG AAGCAGGAAC ACAGCCTCAG
    AAGGGTGTTT CTCAAGTCAA AGAAGGCCCT GTCCTAGCAA CTAGTTCAGG AGCTGGTGTT
    TTTAAGATGG GACGATTTCA GGTTTCTGTT GCAGCAGACG GTGCCCAGAA AGAGGGTAAA
    AATAAGTCAG AAGATGCAAA GTCTGTTCAT TTTGAATCCA GCACCTCAGA GTCCTCAGTG
    CTATCAAGTA GTAGTCCAGA GAGTACCTTG GTGAAACCAG AGCCGAATGG CATAACCATC
    CCTGGTATCT CTTCAGATGT GCCAGAGAGT GCCCACAAAA CTACTGCCTC AGAGGCAAAG
    TCAGACACTG GGCAGCCTAC CAAGGTTGGA CGTTTTCAGG TGACAACTAC AGCAAACAAA
    GTGGGTCGTT TCTCTGTATC AAAAACTGAG GACAAGATCA CTGACACAAA GAAAGAAGGA
    CCAGTGGCAT CTCCTCCTTT TATGGATTTG GAACAAGCTG TTCTTCCTGC TGTGATACCA
    AAGAAAGAGA AGCCTGAACT GTCAGAGCCT TCACATCTAA ATGGGCCGTC TTCTGACCCG
    GAGGCCGCTT TTTTAAGTAG GGATGTGGAT GATGGTTCCG GTAGTCCACA CTCGCCCCAT
    CAGCTGAGCT CAAAGAGCCT TCCTAGCCAG AATCTAAGTC AAAGCCTTAG TAATTCATTT
    AACTCCTCTT ACATGAGTAG CGACAATGAG TCAGATATCG AAGATGAAGA CTTAAAGTTA
    GAGCTGCGAC GACTACGAGA TAAACATCTC AAAGAGATTC AGGACCTGCA GAGTCGCCAG
    AAGCATGAAA TTGAATCTTT GTATACCAAA CTGGGCAAGG TGCCCCCTGC TGTTATTATT
    CCCCCAGCTG CTCCCCTTTC AGGGAGAAGA CGACGACCCA CTAAAAGCAA AGGCAGCAAA
    TCTAGTCGAA GCAGTTCCTT GGGGAATAAA AGCCCCCAGC TTTCAGGTAA CCTGTCTGGT
    CAGAGTGCAG CTTCAGTCTT GCACCCCCAG CAGACCCTCC ACCCTCCTGG CAACATCCCA
    GAGTCCGGGC AGAATCAGCT GTTACAGCCC CTTAAGCCAT CTCCCTCCAG TGACAACCTC
    TATTCAGCCT TCACCAGTGA TGGTGCCATT TCAGTACCAA GCCTTTCTGC TCCAGGTCAA
    GGAACCAGCA GCACAAACAC TGTTGGGGCA ACAGTGAACA GCCAAGCCGC CCAAGCTCAG
    CCTCCTGCCA TGACGTCCAG CAGGAAGGGC ACATTCACAG ATGACTTGCA CAAGTTGGTA
    GACAATTGGG CCCGAGATGC CATGAATCTC TCAGGCAGGA GAGGAAGCAA AGGGCACATG
    AATTACGAGG GCCCTGGAAT GGCAAGGAAG TTCTCTGCAC CTGGGCAACT GTGCATCTCC
    ATGACCTCGA ACCTGGGTGG CTCTGCCCCC ATCTCTGCAG CATCAGCTAC CTCTCTAGGT
    CACTTCACCA AGTCTATGTG CCCCCCACAG CAGTATGGCT TTCCAGCTAC CCCATTTGGC
    GCTCAATGGA GTGGGACGGG TGGCCCAGCA CCACAGCCAC TTGGCCAGTT CCAACCTGTG
    GGAACTGCCT CCTTGCAGAA TTTCAACATC AGCAATTTGC AGAAATCCAT CAGCAACCCC
    CCAGGCTCCA ACCTGCGGAC CACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011519306.1
    CDS969..8615
    Translation

    Target ORF information:

    RefSeq Version XM_011521004.2
    Organism Homo sapiens(human)
    Definition Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011521004.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG
    TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT
    CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT
    AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT
    GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT
    ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT
    ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA
    GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG
    GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA
    CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT
    CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG
    GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG
    AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG
    AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA
    GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT
    CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA
    GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG
    AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC
    CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC
    GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC
    TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA
    TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA
    ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGGGAAT ACAGCAGACA
    GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC
    ACTACTGCAC AGCCAGTGAG TCAGCCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT
    GGAAAACAGA GTACTCAGGG AGTCTCTCAG GTTGCTCCTG CAGAGCCAGT TGCAGTAGCA
    CAGACCCAAG CTACCCAGCC GACCACTTTG GCTTCCTCTG TAGACAGTGC ACATTCAGAT
    GTTGCTTCAG GTATGAGTGA TGGCAATGAG AACGTCCCAT CTTCCAGTGG AAGGCATGAA
    GGAAGAACTA CAAAACGGCA TTACCGAAAA TCTGTAAGGA GTCGCTCTCG ACATGAAAAA
    ACTTCACGCC CAAAATTAAG AATTTTGAAT GTTTCAAATA AAGGAGACCG AGTAGTAGAA
    TGTCAATTAG AGACTCATAA TAGGAAAATG GTTACATTCA AATTTGACCT AGATGGTGAC
    AACCCCGAGG AGATAGCAAC AATTATGGTG AACAATGACT TTATTCTAGC AATAGAGAGA
    GAGTCGTTTG TGGATCAAGT GCGAGAAATT ATTGAAAAAG CTGATGAAAT GCTCAGTGAG
    GATGTCAGTG TGGAACCAGA GGGTGATCAG GGATTGGAGA GTCTACAAGG AAAGGATGAC
    TATGGCTTTT CAGGTTCTCA GAAATTGGAA GGAGAGTTCA AACAACCAAT TCCTGCGTCT
    TCCATGCCAC AGCAAATAGG CATTCCTACC AGTTCTTTAA CTCAAGTTGT TCATTCTGCG
    GGAAGGCGGT TTATAGTGAG TCCTGTGCCA GAAAGCCGAT TACGAGAATC AAAAGTTTTC
    CCCAGTGAAA TAACAGATAC AGTTGCTGCC TCTACAGCTC AGAGCCCTGG AATGAACTTG
    TCTCACTCTG CATCATCCCT TAGTCTACAA CAGGCCTTTT CTGAACTTAG ACGTGCCCAA
    ATGACAGAAG GACCCAACAC AGCACCTCCA AACTTTAGTC ATACAGGACC AACATTTCCA
    GTAGTACCTC CTTTCTTAAG TAGCATTGCT GGAGTCCCAA CCACAGCAGC AGCCACAGCA
    CCAGTCCCTG CAACAAGCAG CCCTCCTAAT GACATTTCCA CATCAGTAAT TCAGTCTGAG
    GTTACAGTGC CCACTGAAGA GGGGATTGCT GGAGTTGCCA CCAGCACAGG TGTGGTAACT
    TCAGGTGGTC TCCCCATACC ACCTGTGTCT GAATCACCAG TACTTTCCAG CGTAGTTTCA
    AGTATCACAA TACCTGCAGT TGTCTCAATA TCTACTACAT CCCCGTCACT TCAAGTCCCC
    ACATCCACAT CTGAGATCGT TGTTTCTAGT ACAGCACTGT ATCCTTCAGT AACAGTTTCA
    GCAACTTCAG CCTCTGCAGG GGGCAGTACT GCTACCCCAG GTCCTAAGCC TCCAGCTGTA
    GTATCTCAGC AGGCAGCAGG CAGCACTACT GTGGGAGCCA CATTAACATC AGTTTCTACC
    ACCACTTCAT TCCCAAGCAC AGCTTCACAG CTGTGCATTC AGCTTAGCAG CAGTACTTCT
    ACTCCTACTT TAGCTGAAAC CGTGGTAGTT AGCGCACACT CACTAGATAA GACATCTCAT
    AGCAGTACAA CTGGATTGGC TTTCTCCCTC TCTGCACCAT CTTCCTCTTC CTCTCCTGGA
    GCAGGAGTGT CTAGTTATAT TTCTCAGCCT GGTGGGCTGC ATCCTTTGGT CATTCCATCA
    GTGATAGCTT CTACTCCTAT TCTTCCCCAA GCAGCAGGAC CTACTTCTAC ACCTTTATTA
    CCCCAAGTAC CTAGTATCCC ACCCTTGGTA CAGCCTGTTG CCAATGTGCC TGCTGTACAG
    CAGACACTAA TTCATAGTCA GCCTCAACCA GCTTTGCTTC CCAACCAGCC CCATACTCAT
    TGTCCTGAAG TAGATTCTGA TACACAACCC AAAGCTCCTG GAATTGATGA CATAAAGACT
    CTAGAAGAAA AGCTGCGGTC TCTGTTCAGT GAACACAGCT CATCTGGAGC TCAGCATGCC
    TCTGTCTCAC TGGAGACCTC ACTAGTCATA GAGAGCACTG TCACACCAGG CATCCCAACT
    ACTGCTGTTG CACCAAGCAA ACTCCTGACT TCTACCACAA GTACTTGCTT ACCACCAACC
    AATTTACCAC TAGGAACAGT TGCTTTGCCA GTTACACCAG TGGTCACACC TGGGCAAGTT
    TCTACCCCAG TCAGCACTAC TACATCAGGA GTGAAACCTG GAACTGCTCC CTCCAAGCCA
    CCTCTAACTA AGGCTCCGGT GCTGCCAGTG GGTACTGAAC TTCCAGCAGG TACTCTACCC
    AGCGAGCAGC TGCCACCTTT TCCAGGACCT TCTCTAACCC AGTCCCAGCA ACCTCTAGAG
    GATCTTGATG CTCAATTGAG AAGAACACTT AGTCCAGAGA TGATCACAGT GACTTCTGCG
    GTTGGTCCTG TGTCCATGGC GGCTCCAACA GCAATCACAG AAGCAGGAAC ACAGCCTCAG
    AAGGGTGTTT CTCAAGTCAA AGAAGGCCCT GTCCTAGCAA CTAGTTCAGG AGCTGGTGTT
    TTTAAGATGG GACGATTTCA GGTTTCTGTT GCAGCAGACG GTGCCCAGAA AGAGGGTAAA
    AATAAGTCAG AAGATGCAAA GTCTGTTCAT TTTGAATCCA GCACCTCAGA GTCCTCAGTG
    CTATCAAGTA GTAGTCCAGA GAGTACCTTG GTGAAACCAG AGCCGAATGG CATAACCATC
    CCTGGTATCT CTTCAGATGT GCCAGAGAGT GCCCACAAAA CTACTGCCTC AGAGGCAAAG
    TCAGACACTG GGCAGCCTAC CAAGGTTGGA CGTTTTCAGG TGACAACTAC AGCAAACAAA
    GTGGGTCGTT TCTCTGTATC AAAAACTGAG GACAAGATCA CTGACACAAA GAAAGAAGGA
    CCAGTGGCAT CTCCTCCTTT TATGGATTTG GAACAAGCTG TTCTTCCTGC TGTGATACCA
    AAGAAAGAGA AGCCTGAACT GTCAGAGCCT TCACATCTAA ATGGGCCGTC TTCTGACCCG
    GAGGCCGCTT TTTTAAGTAG GGATGTGGAT GATGGTTCCG GTAGTCCACA CTCGCCCCAT
    CAGCTGAGCT CAAAGAGCCT TCCTAGCCAG AATCTAAGTC AAAGCCTTAG TAATTCATTT
    AACTCCTCTT ACATGAGTAG CGACAATGAG TCAGATATCG AAGATGAAGA CTTAAAGTTA
    GAGCTGCGAC GACTACGAGA TAAACATCTC AAAGAGATTC AGGACCTGCA GAGTCGCCAG
    AAGCATGAAA TTGAATCTTT GTATACCAAA CTGGGCAAGG TGCCCCCTGC TGTTATTATT
    CCCCCAGCTG CTCCCCTTTC AGGGAGAAGA CGACGACCCA CTAAAAGCAA AGGCAGCAAA
    TCTAGTCGAA GCAGTTCCTT GGGGAATAAA AGCCCCCAGC TTTCAGGTAA CCTGTCTGGT
    CAGAGTGCAG CTTCAGTCTT GCACCCCCAG CAGACCCTCC ACCCTCCTGG CAACATCCCA
    GAGTCCGGGC AGAATCAGCT GTTACAGCCC CTTAAGCCAT CTCCCTCCAG TGACAACCTC
    TATTCAGCCT TCACCAGTGA TGGTGCCATT TCAGTACCAA GCCTTTCTGC TCCAGGTCAA
    GGAACCAGCA GCACAAACAC TGTTGGGGCA ACAGTGAACA GCCAAGCCGC CCAAGCTCAG
    CCTCCTGCCA TGACGTCCAG CAGGAAGGGC ACATTCACAG ATGACTTGCA CAAGTTGGTA
    GACAATTGGG CCCGAGATGC CATGAATCTC TCAGGCAGGA GAGGAAGCAA AGGGCACATG
    AATTACGAGG GCCCTGGAAT GGCAAGGAAG TTCTCTGCAC CTGGGCAACT GTGCATCTCC
    ATGACCTCGA ACCTGGGTGG CTCTGCCCCC ATCTCTGCAG CATCAGCTAC CTCTCTAGGT
    CACTTCACCA AGTCTATGTG CCCCCCACAG CAGTATGGCT TTCCAGCTAC CCCATTTGGC
    GCTCAATGGA GTGGGACGGG TGGCCCAGCA CCACAGCCAC TTGGCCAGTT CCAACCTGTG
    GGAACTGCCT CCTTGCAGAA TTTCAACATC AGCAATTTGC AGAAATCCAT CAGCAACCCC
    CCAGGCTCCA ACCTGCGGAC CACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71621
    Clone ID Related Accession (Same CDS sequence) XM_011521005.2
    Accession Version XM_011521005.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7167bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase WNK1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521005.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGGATATTA AAAAGAAAGA TTTTTGCTCA GTATTTGTCA TCATAAATTC TCATTGCTGC 
    TGCTGCCCTC AAAAGGATTG TATAAATGAG GGGGTGAAGC CAGCCAGTTT TGACAAAGTA
    GCAATTCCTG AAGTGAAGGA AATTATTGAA GGATGCATAC GACAAAACAA AGATGAAAGA
    TATTCCATCA AAGACCTTTT GAACCATGCC TTCTTCCAAG AGGAAACAGG AGTACGGGTA
    GAATTAGCAG AAGAAGATGA TGGAGAAAAA ATAGCCATAA AATTATGGCT ACGTATTGAA
    GATATTAAGA AATTAAAGGG AAAATACAAA GATAATGAAG CTATTGAGTT TTCTTTTGAT
    TTAGAGAGAG ATGTCCCAGA AGATGTTGCA CAAGAAATGG TAGAGTCTGG GTATGTCTGT
    GAAGGTGATC ACAAGACCAT GGCTAAAGCT ATCAAAGACA GAGTATCATT AATTAAGAGG
    AAACGAGAGC AGCGGCAGTT GGTACGGGAG GAGCAAGAAA AAAAAAAGCA GGAAGAGAGC
    AGTCTCAAAC AGCAGGTAGA ACAATCCAGT GCTTCCCAGA CAGGAATCAA GCAGCTCCCT
    TCTGCTAGCA CCGGCATACC TACTGCTTCT ACCACTTCAG CTTCAGTTTC TACACAAGTA
    GAACCTGAAG AACCTGAGGC AGATCAACAT CAACAACTAC AGTACCAGCA ACCCAGTATA
    TCTGTGTTAT CTGATGGGAC GGTTGACAGT GGTCAGGGAT CCTCTGTCTT CACAGAATCT
    CGAGTGAGCA GCCAACAGAC AGTTTCATAT GGTTCCCAAC ATGAACAGGC ACATTCTACA
    GGCACAGTCC CAGGGCATAT ACCTTCTACT GTCCAAGCAC AGTCTCAGCC CCATGGGGTA
    TATCCACCCT CAAGTGTGCC TCAGTCCATG GCGCATCCGT GTGGGGGGAC CCCAACATAC
    CCAGAATCAC AGATATTTTT CCCAACTATT CATGAACGTC CAGTTTCTTT TTCACCACCT
    CCCACCTGCC CACCGAAAGT AGCCATTTCC CAGCGGCGTA AGAGCACCTC CTTCCTGGAA
    GCCCAAACTC ACCACTTCCA ACCCCTGCTG AGGACTGTTG GCCAAAGTCT TCTTCCACCT
    GGTGGCAGCC CAACTAACTG GACACCAGAG GCCGTAGTTA TGTTGGGTAC TACAGCCAGT
    AGAGTAACTG GAGAGTCATG TGAGATACAG GTCCATCCTA TGTTTGAACC ATCTCAAGTT
    TACAGTGACT ATAGACCTGG ACTAGTACTT CCAGAAGAAG CTCACTATTT TATTCCTCAG
    GAAGCAGTGT ATGTAGCTGG GGTACATTAC CAGGCCCGGG TGGCAGAACA GTATGAGGGC
    ATTCCATACA ACTCATCAGT ACTGTCAAGT CCTATGAAAC AGATACCTGA ACAGAAGCCA
    GTACAAGGGG GCCCTACTTC AAGTTCTGTC TTTGAATTTC CATCTGGACA GGCTTTCCTG
    GTAGGACACC TTCAGAATCT AAGATTAGAT TCTGGATTGG GTCCGGGATC TCCCCTCTCT
    AGTATTTCTG CACCTATCAG TACAGATGCT ACACGTTTGA AATTTCACCC TGTCTTTGTT
    CCTCATTCTG CGCCTGCTGT GTTAACTCAT AACAATGAGA GCAGAAGCAA CTGTGTATTT
    GAATTTCATG TTCACACACC AAGCTCCTCT TCAGGAGAAG GAGGTGGAAT TTTACCTCAG
    CGTGTTTACC GAAATCGGCA GGTTGCAGTG GACTTGAATC AAGAAGAACT GCCTCCTCAA
    TCAGTTGGAT TACATGGCTA CTTGCAGCCT GTGACTGAAG AAAAGCATAA TTACCATGCC
    CCAGAATTGA CCGTTTCTGT GGTAGAGCCT ATCGGACAGA ACTGGCCAAT AGGAAGCCCA
    GAATATTCCA GTGATTCCTC ACAAATCACT TCTTCAGACC CCAGTGATTT TCAGTCACCT
    CCCCCTACAG GGGGAGCAGC TGCACCTTTT GGCTCTGACG TCTCAATGCC CTTTATCCAT
    CTGCCTCAGA CAGTGTTACA AGAATCCCCA CTTTTCTTCT GTTTCCCCCA AGGAACCACA
    TCTCAGCAGG TCTTAACTGC CTCATTTTCT TCAGGAGGAT CTGCACTTCA TCCACAGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCCTCA AGTAGCTTAA CAGGGGTTTC ATCTTCCCAA
    CCCATACAAC ATCCTCAGCA GCAGCAGGGA ATACAGCAGA CAGCCCCTCC TCAACAGACA
    GTGCAGTATT CACTTTCACA GACATCAACC TCCAGTGAGG CCACTACTGC ACAGCCAGTG
    AGTCAGCCTC AAGCTCCACA AGTCTTGCCT CAAGTATCAG CTGGAAAACA GCTTCCAGTT
    TCCCAGCCAG TACCAACTAT CCAAGGCGAA CCTCAGATCC CAGTTGCGAC ACAACCCTCG
    GTTGTTCCAG TCCACTCTGG TGCTCATTTC CTTCCAGTGG GACAGCCGCT CCCTACTCCC
    TTGCTCCCTC AGTACCCTGT CTCTCAGATT CCCATATCAA CTCCTCATGT GTCTACGGCT
    CAGACAGGTT TCTCATCCCT TCCCATCACA ATGGCAGCTG GCATTACTCA GCCTCTGCTC
    ACGTTGGCTT CATCTGCTAC AACAGCTGCG ATCCCGGGGG TATCAACTGT GGTTCCTAGT
    CAGCTTCCAA CCCTTCTGCA GCCTGTGACT CAGCTGCCAA GTCAGGTTCA CCCACAGCTC
    CTACAACCAG CAGTTCAGTC CATGGGAATA CCAGCTAACC TTGGACAAGC TGCTGAGGTT
    CCACTTTCCT CTGGAGATGT TCTGTACCAG GGCTTCCCAC CTCGACTGCC ACCACAGTAC
    CCAGGAGATT CAAATATTGC TCCCTCTTCC AACGTGGCTT CTGTTTGCAT CCATTCTACA
    GTCCTATCCC CTCCCATGCC GACAGAAGTA CTGGCTACAC CTGGGTACTT TCCCACAGTG
    GTGCAGCCTT ATGTGGAATC AAATCTTTTA GTTCCTATGG GTGGTGTAGG AGGACAGGTT
    CAAGTGTCCC AGCCAGGAGG GAGTTTAGCA CAAGCCCCCA CTACATCCTC CCAGCAAGCA
    GTTTTGGAGA GTACTCAGGG AGTCTCTCAG GTTGCTCCTG CAGAGCCAGT TGCAGTAGCA
    CAGACCCAAG CTACCCAGCC GACCACTTTG GCTTCCTCTG TAGACAGTGC ACATTCAGAT
    GTTGCTTCAG GTATGAGTGA TGGCAATGAG AACGTCCCAT CTTCCAGTGG AAGGCATGAA
    GGAAGAACTA CAAAACGGCA TTACCGAAAA TCTGTAAGGA GTCGCTCTCG ACATGAAAAA
    ACTTCACGCC CAAAATTAAG AATTTTGAAT GTTTCAAATA AAGGAGACCG AGTAGTAGAA
    TGTCAATTAG AGACTCATAA TAGGAAAATG GTTACATTCA AATTTGACCT AGATGGTGAC
    AACCCCGAGG AGATAGCAAC AATTATGGTG AACAATGACT TTATTCTAGC AATAGAGAGA
    GAGTCGTTTG TGGATCAAGT GCGAGAAATT ATTGAAAAAG CTGATGAAAT GCTCAGTGAG
    GATGTCAGTG TGGAACCAGA GGGTGATCAG GGATTGGAGA GTCTACAAGG AAAGGATGAC
    TATGGCTTTT CAGGTTCTCA GAAATTGGAA GGAGAGTTCA AACAACCAAT TCCTGCGTCT
    TCCATGCCAC AGCAAATAGG CATTCCTACC AGTTCTTTAA CTCAAGTTGT TCATTCTGCG
    GGAAGGCGGT TTATAGTGAG TCCTGTGCCA GAAAGCCGAT TACGAGAATC AAAAGTTTTC
    CCCAGTGAAA TAACAGATAC AGTTGCTGCC TCTACAGCTC AGAGCCCTGG AATGAACTTG
    TCTCACTCTG CATCATCCCT TAGTCTACAA CAGGCCTTTT CTGAACTTAG ACGTGCCCAA
    ATGACAGAAG GACCCAACAC AGCACCTCCA AACTTTAGTC ATACAGGACC AACATTTCCA
    GTAGTACCTC CTTTCTTAAG TAGCATTGCT GGAGTCCCAA CCACAGCAGC AGCCACAGCA
    CCAGTCCCTG CAACAAGCAG CCCTCCTAAT GACATTTCCA CATCAGTAAT TCAGTCTGAG
    GTTACAGTGC CCACTGAAGA GGGGATTGCT GGAGTTGCCA CCAGCACAGG TGTGGTAACT
    TCAGGTGGTC TCCCCATACC ACCTGTGTCT GAATCACCAG TACTTTCCAG CGTAGTTTCA
    AGTATCACAA TACCTGCAGT TGTCTCAATA TCTACTACAT CCCCGTCACT TCAAGTCCCC
    ACATCCACAT CTGAGATCGT TGTTTCTAGT ACAGCACTGT ATCCTTCAGT AACAGTTTCA
    GCAACTTCAG CCTCTGCAGG GGGCAGTACT GCTACCCCAG GTCCTAAGCC TCCAGCTGTA
    GTATCTCAGC AGGCAGCAGG CAGCACTACT GTGGGAGCCA CATTAACATC AGTTTCTACC
    ACCACTTCAT TCCCAAGCAC AGCTTCACAG CTGTGCATTC AGCTTAGCAG CAGTACTTCT
    ACTCCTACTT TAGCTGAAAC CGTGGTAGTT AGCGCACACT CACTAGATAA GACATCTCAT
    AGCAGTACAA CTGGATTGGC TTTCTCCCTC TCTGCACCAT CTTCCTCTTC CTCTCCTGGA
    GCAGGAGTGT CTAGTTATAT TTCTCAGCCT GGTGGGCTGC ATCCTTTGGT CATTCCATCA
    GTGATAGCTT CTACTCCTAT TCTTCCCCAA GCAGCAGGAC CTACTTCTAC ACCTTTATTA
    CCCCAAGTAC CTAGTATCCC ACCCTTGGTA CAGCCTGTTG CCAATGTGCC TGCTGTACAG
    CAGACACTAA TTCATAGTCA GCCTCAACCA GCTTTGCTTC CCAACCAGCC CCATACTCAT
    TGTCCTGAAG TAGATTCTGA TACACAACCC AAAGCTCCTG GAATTGATGA CATAAAGACT
    CTAGAAGAAA AGCTGCGGTC TCTGTTCAGT GAACACAGCT CATCTGGAGC TCAGCATGCC
    TCTGTCTCAC TGGAGACCTC ACTAGTCATA GAGAGCACTG TCACACCAGG CATCCCAACT
    ACTGCTGTTG CACCAAGCAA ACTCCTGACT TCTACCACAA GTACTTGCTT ACCACCAACC
    AATTTACCAC TAGGAACAGT TGCTTTGCCA GTTACACCAG TGGTCACACC TGGGCAAGTT
    TCTACCCCAG TCAGCACTAC TACATCAGGA GTGAAACCTG GAACTGCTCC CTCCAAGCCA
    CCTCTAACTA AGGCTCCGGT GCTGCCAGTG GGTACTGAAC TTCCAGCAGG TACTCTACCC
    AGCGAGCAGC TGCCACCTTT TCCAGGACCT TCTCTAACCC AGTCCCAGCA ACCTCTAGAG
    GATCTTGATG CTCAATTGAG AAGAACACTT AGTCCAGAGA TGATCACAGT GACTTCTGCG
    GTTGGTCCTG TGTCCATGGC GGCTCCAACA GCAATCACAG AAGCAGGAAC ACAGCCTCAG
    AAGGGTGTTT CTCAAGTCAA AGAAGGCCCT GTCCTAGCAA CTAGTTCAGG AGCTGGTGTT
    TTTAAGATGG GACGATTTCA GGTTTCTGTT GCAGCAGACG GTGCCCAGAA AGAGGGTAAA
    AATAAGTCAG AAGATGCAAA GTCTGTTCAT TTTGAATCCA GCACCTCAGA GTCCTCAGTG
    CTATCAAGTA GTAGTCCAGA GAGTACCTTG GTGAAACCAG AGCCGAATGG CATAACCATC
    CCTGGTATCT CTTCAGATGT GCCAGAGAGT GCCCACAAAA CTACTGCCTC AGAGGCAAAG
    TCAGACACTG GGCAGCCTAC CAAGGTTGGA CGTTTTCAGG TGACAACTAC AGCAAACAAA
    GTGGGTCGTT TCTCTGTATC AAAAACTGAG GACAAGATCA CTGACACAAA GAAAGAAGGA
    CCAGTGGCAT CTCCTCCTTT TATGGATTTG GAACAAGCTG TTCTTCCTGC TGTGATACCA
    AAGAAAGAGA AGCCTGAACT GTCAGAGCCT TCACATCTAA ATGGGCCGTC TTCTGACCCG
    GAGGCCGCTT TTTTAAGTAG GGATGTGGAT GATGGTTCCG GTAGTCCACA CTCGCCCCAT
    CAGCTGAGCT CAAAGAGCCT TCCTAGCCAG AATCTAAGTC AAAGCCTTAG TAATTCATTT
    AACTCCTCTT ACATGAGTAG CGACAATGAG TCAGATATCG AAGATGAAGA CTTAAAGTTA
    GAGCTGCGAC GACTACGAGA TAAACATCTC AAAGAGATTC AGGACCTGCA GAGTCGCCAG
    AAGCATGAAA TTGAATCTTT GTATACCAAA CTGGGCAAGG TGCCCCCTGC TGTTATTATT
    CCCCCAGCTG CTCCCCTTTC AGGGAGAAGA CGACGACCCA CTAAAAGCAA AGGCAGCAAA
    TCTAGTCGAA GCAGTTCCTT GGGGAATAAA AGCCCCCAGC TTTCAGGTAA CCTGTCTGGT
    CAGAGTGCAG CTTCAGTCTT GCACCCCCAG CAGACCCTCC ACCCTCCTGG CAACATCCCA
    GAGTCCGGGC AGAATCAGCT GTTACAGCCC CTTAAGCCAT CTCCCTCCAG TGACAACCTC
    TATTCAGCCT TCACCAGTGA TGGTGCCATT TCAGTACCAA GCCTTTCTGC TCCAGGTCAA
    GGAACCAGCA GCACAAACAC TGTTGGGGCA ACAGTGAACA GCCAAGCCGC CCAAGCTCAG
    CCTCCTGCCA TGACGTCCAG CAGGAAGGGC ACATTCACAG ATGACTTGCA CAAGTTGGTA
    GACAATTGGG CCCGAGATGC CATGAATCTC TCAGGCAGGA GAGGAAGCAA AGGGCACATG
    AATTACGAGG GCCCTGGAAT GGCAAGGAAG TTCTCTGCAC CTGGGCAACT GTGCATCTCC
    ATGACCTCGA ACCTGGGTGG CTCTGCCCCC ATCTCTGCAG CATCAGCTAC CTCTCTAGGT
    CACTTCACCA AGTCTATGTG CCCCCCACAG CAGTATGGCT TTCCAGCTAC CCCATTTGGC
    GCTCAATGGA GTGGGACGGG TGGCCCAGCA CCACAGCCAC TTGGCCAGTT CCAACCTGTG
    GGAACTGCCT CCTTGCAGAA TTTCAACATC AGCAATTTGC AGAAATCCAT CAGCAACCCC
    CCAGGCTCCA ACCTGCGGAC CACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011519307.1
    CDS674..7840
    Translation

    Target ORF information:

    RefSeq Version XM_011521005.2
    Organism Homo sapiens(human)
    Definition Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011521005.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGGATATTA AAAAGAAAGA TTTTTGCTCA GTATTTGTCA TCATAAATTC TCATTGCTGC 
    TGCTGCCCTC AAAAGGATTG TATAAATGAG GGGGTGAAGC CAGCCAGTTT TGACAAAGTA
    GCAATTCCTG AAGTGAAGGA AATTATTGAA GGATGCATAC GACAAAACAA AGATGAAAGA
    TATTCCATCA AAGACCTTTT GAACCATGCC TTCTTCCAAG AGGAAACAGG AGTACGGGTA
    GAATTAGCAG AAGAAGATGA TGGAGAAAAA ATAGCCATAA AATTATGGCT ACGTATTGAA
    GATATTAAGA AATTAAAGGG AAAATACAAA GATAATGAAG CTATTGAGTT TTCTTTTGAT
    TTAGAGAGAG ATGTCCCAGA AGATGTTGCA CAAGAAATGG TAGAGTCTGG GTATGTCTGT
    GAAGGTGATC ACAAGACCAT GGCTAAAGCT ATCAAAGACA GAGTATCATT AATTAAGAGG
    AAACGAGAGC AGCGGCAGTT GGTACGGGAG GAGCAAGAAA AAAAAAAGCA GGAAGAGAGC
    AGTCTCAAAC AGCAGGTAGA ACAATCCAGT GCTTCCCAGA CAGGAATCAA GCAGCTCCCT
    TCTGCTAGCA CCGGCATACC TACTGCTTCT ACCACTTCAG CTTCAGTTTC TACACAAGTA
    GAACCTGAAG AACCTGAGGC AGATCAACAT CAACAACTAC AGTACCAGCA ACCCAGTATA
    TCTGTGTTAT CTGATGGGAC GGTTGACAGT GGTCAGGGAT CCTCTGTCTT CACAGAATCT
    CGAGTGAGCA GCCAACAGAC AGTTTCATAT GGTTCCCAAC ATGAACAGGC ACATTCTACA
    GGCACAGTCC CAGGGCATAT ACCTTCTACT GTCCAAGCAC AGTCTCAGCC CCATGGGGTA
    TATCCACCCT CAAGTGTGCC TCAGTCCATG GCGCATCCGT GTGGGGGGAC CCCAACATAC
    CCAGAATCAC AGATATTTTT CCCAACTATT CATGAACGTC CAGTTTCTTT TTCACCACCT
    CCCACCTGCC CACCGAAAGT AGCCATTTCC CAGCGGCGTA AGAGCACCTC CTTCCTGGAA
    GCCCAAACTC ACCACTTCCA ACCCCTGCTG AGGACTGTTG GCCAAAGTCT TCTTCCACCT
    GGTGGCAGCC CAACTAACTG GACACCAGAG GCCGTAGTTA TGTTGGGTAC TACAGCCAGT
    AGAGTAACTG GAGAGTCATG TGAGATACAG GTCCATCCTA TGTTTGAACC ATCTCAAGTT
    TACAGTGACT ATAGACCTGG ACTAGTACTT CCAGAAGAAG CTCACTATTT TATTCCTCAG
    GAAGCAGTGT ATGTAGCTGG GGTACATTAC CAGGCCCGGG TGGCAGAACA GTATGAGGGC
    ATTCCATACA ACTCATCAGT ACTGTCAAGT CCTATGAAAC AGATACCTGA ACAGAAGCCA
    GTACAAGGGG GCCCTACTTC AAGTTCTGTC TTTGAATTTC CATCTGGACA GGCTTTCCTG
    GTAGGACACC TTCAGAATCT AAGATTAGAT TCTGGATTGG GTCCGGGATC TCCCCTCTCT
    AGTATTTCTG CACCTATCAG TACAGATGCT ACACGTTTGA AATTTCACCC TGTCTTTGTT
    CCTCATTCTG CGCCTGCTGT GTTAACTCAT AACAATGAGA GCAGAAGCAA CTGTGTATTT
    GAATTTCATG TTCACACACC AAGCTCCTCT TCAGGAGAAG GAGGTGGAAT TTTACCTCAG
    CGTGTTTACC GAAATCGGCA GGTTGCAGTG GACTTGAATC AAGAAGAACT GCCTCCTCAA
    TCAGTTGGAT TACATGGCTA CTTGCAGCCT GTGACTGAAG AAAAGCATAA TTACCATGCC
    CCAGAATTGA CCGTTTCTGT GGTAGAGCCT ATCGGACAGA ACTGGCCAAT AGGAAGCCCA
    GAATATTCCA GTGATTCCTC ACAAATCACT TCTTCAGACC CCAGTGATTT TCAGTCACCT
    CCCCCTACAG GGGGAGCAGC TGCACCTTTT GGCTCTGACG TCTCAATGCC CTTTATCCAT
    CTGCCTCAGA CAGTGTTACA AGAATCCCCA CTTTTCTTCT GTTTCCCCCA AGGAACCACA
    TCTCAGCAGG TCTTAACTGC CTCATTTTCT TCAGGAGGAT CTGCACTTCA TCCACAGGCA
    CAGGGGCAGA GCCAGGGTCA GCCATCCTCA AGTAGCTTAA CAGGGGTTTC ATCTTCCCAA
    CCCATACAAC ATCCTCAGCA GCAGCAGGGA ATACAGCAGA CAGCCCCTCC TCAACAGACA
    GTGCAGTATT CACTTTCACA GACATCAACC TCCAGTGAGG CCACTACTGC ACAGCCAGTG
    AGTCAGCCTC AAGCTCCACA AGTCTTGCCT CAAGTATCAG CTGGAAAACA GCTTCCAGTT
    TCCCAGCCAG TACCAACTAT CCAAGGCGAA CCTCAGATCC CAGTTGCGAC ACAACCCTCG
    GTTGTTCCAG TCCACTCTGG TGCTCATTTC CTTCCAGTGG GACAGCCGCT CCCTACTCCC
    TTGCTCCCTC AGTACCCTGT CTCTCAGATT CCCATATCAA CTCCTCATGT GTCTACGGCT
    CAGACAGGTT TCTCATCCCT TCCCATCACA ATGGCAGCTG GCATTACTCA GCCTCTGCTC
    ACGTTGGCTT CATCTGCTAC AACAGCTGCG ATCCCGGGGG TATCAACTGT GGTTCCTAGT
    CAGCTTCCAA CCCTTCTGCA GCCTGTGACT CAGCTGCCAA GTCAGGTTCA CCCACAGCTC
    CTACAACCAG CAGTTCAGTC CATGGGAATA CCAGCTAACC TTGGACAAGC TGCTGAGGTT
    CCACTTTCCT CTGGAGATGT TCTGTACCAG GGCTTCCCAC CTCGACTGCC ACCACAGTAC
    CCAGGAGATT CAAATATTGC TCCCTCTTCC AACGTGGCTT CTGTTTGCAT CCATTCTACA
    GTCCTATCCC CTCCCATGCC GACAGAAGTA CTGGCTACAC CTGGGTACTT TCCCACAGTG
    GTGCAGCCTT ATGTGGAATC AAATCTTTTA GTTCCTATGG GTGGTGTAGG AGGACAGGTT
    CAAGTGTCCC AGCCAGGAGG GAGTTTAGCA CAAGCCCCCA CTACATCCTC CCAGCAAGCA
    GTTTTGGAGA GTACTCAGGG AGTCTCTCAG GTTGCTCCTG CAGAGCCAGT TGCAGTAGCA
    CAGACCCAAG CTACCCAGCC GACCACTTTG GCTTCCTCTG TAGACAGTGC ACATTCAGAT
    GTTGCTTCAG GTATGAGTGA TGGCAATGAG AACGTCCCAT CTTCCAGTGG AAGGCATGAA
    GGAAGAACTA CAAAACGGCA TTACCGAAAA TCTGTAAGGA GTCGCTCTCG ACATGAAAAA
    ACTTCACGCC CAAAATTAAG AATTTTGAAT GTTTCAAATA AAGGAGACCG AGTAGTAGAA
    TGTCAATTAG AGACTCATAA TAGGAAAATG GTTACATTCA AATTTGACCT AGATGGTGAC
    AACCCCGAGG AGATAGCAAC AATTATGGTG AACAATGACT TTATTCTAGC AATAGAGAGA
    GAGTCGTTTG TGGATCAAGT GCGAGAAATT ATTGAAAAAG CTGATGAAAT GCTCAGTGAG
    GATGTCAGTG TGGAACCAGA GGGTGATCAG GGATTGGAGA GTCTACAAGG AAAGGATGAC
    TATGGCTTTT CAGGTTCTCA GAAATTGGAA GGAGAGTTCA AACAACCAAT TCCTGCGTCT
    TCCATGCCAC AGCAAATAGG CATTCCTACC AGTTCTTTAA CTCAAGTTGT TCATTCTGCG
    GGAAGGCGGT TTATAGTGAG TCCTGTGCCA GAAAGCCGAT TACGAGAATC AAAAGTTTTC
    CCCAGTGAAA TAACAGATAC AGTTGCTGCC TCTACAGCTC AGAGCCCTGG AATGAACTTG
    TCTCACTCTG CATCATCCCT TAGTCTACAA CAGGCCTTTT CTGAACTTAG ACGTGCCCAA
    ATGACAGAAG GACCCAACAC AGCACCTCCA AACTTTAGTC ATACAGGACC AACATTTCCA
    GTAGTACCTC CTTTCTTAAG TAGCATTGCT GGAGTCCCAA CCACAGCAGC AGCCACAGCA
    CCAGTCCCTG CAACAAGCAG CCCTCCTAAT GACATTTCCA CATCAGTAAT TCAGTCTGAG
    GTTACAGTGC CCACTGAAGA GGGGATTGCT GGAGTTGCCA CCAGCACAGG TGTGGTAACT
    TCAGGTGGTC TCCCCATACC ACCTGTGTCT GAATCACCAG TACTTTCCAG CGTAGTTTCA
    AGTATCACAA TACCTGCAGT TGTCTCAATA TCTACTACAT CCCCGTCACT TCAAGTCCCC
    ACATCCACAT CTGAGATCGT TGTTTCTAGT ACAGCACTGT ATCCTTCAGT AACAGTTTCA
    GCAACTTCAG CCTCTGCAGG GGGCAGTACT GCTACCCCAG GTCCTAAGCC TCCAGCTGTA
    GTATCTCAGC AGGCAGCAGG CAGCACTACT GTGGGAGCCA CATTAACATC AGTTTCTACC
    ACCACTTCAT TCCCAAGCAC AGCTTCACAG CTGTGCATTC AGCTTAGCAG CAGTACTTCT
    ACTCCTACTT TAGCTGAAAC CGTGGTAGTT AGCGCACACT CACTAGATAA GACATCTCAT
    AGCAGTACAA CTGGATTGGC TTTCTCCCTC TCTGCACCAT CTTCCTCTTC CTCTCCTGGA
    GCAGGAGTGT CTAGTTATAT TTCTCAGCCT GGTGGGCTGC ATCCTTTGGT CATTCCATCA
    GTGATAGCTT CTACTCCTAT TCTTCCCCAA GCAGCAGGAC CTACTTCTAC ACCTTTATTA
    CCCCAAGTAC CTAGTATCCC ACCCTTGGTA CAGCCTGTTG CCAATGTGCC TGCTGTACAG
    CAGACACTAA TTCATAGTCA GCCTCAACCA GCTTTGCTTC CCAACCAGCC CCATACTCAT
    TGTCCTGAAG TAGATTCTGA TACACAACCC AAAGCTCCTG GAATTGATGA CATAAAGACT
    CTAGAAGAAA AGCTGCGGTC TCTGTTCAGT GAACACAGCT CATCTGGAGC TCAGCATGCC
    TCTGTCTCAC TGGAGACCTC ACTAGTCATA GAGAGCACTG TCACACCAGG CATCCCAACT
    ACTGCTGTTG CACCAAGCAA ACTCCTGACT TCTACCACAA GTACTTGCTT ACCACCAACC
    AATTTACCAC TAGGAACAGT TGCTTTGCCA GTTACACCAG TGGTCACACC TGGGCAAGTT
    TCTACCCCAG TCAGCACTAC TACATCAGGA GTGAAACCTG GAACTGCTCC CTCCAAGCCA
    CCTCTAACTA AGGCTCCGGT GCTGCCAGTG GGTACTGAAC TTCCAGCAGG TACTCTACCC
    AGCGAGCAGC TGCCACCTTT TCCAGGACCT TCTCTAACCC AGTCCCAGCA ACCTCTAGAG
    GATCTTGATG CTCAATTGAG AAGAACACTT AGTCCAGAGA TGATCACAGT GACTTCTGCG
    GTTGGTCCTG TGTCCATGGC GGCTCCAACA GCAATCACAG AAGCAGGAAC ACAGCCTCAG
    AAGGGTGTTT CTCAAGTCAA AGAAGGCCCT GTCCTAGCAA CTAGTTCAGG AGCTGGTGTT
    TTTAAGATGG GACGATTTCA GGTTTCTGTT GCAGCAGACG GTGCCCAGAA AGAGGGTAAA
    AATAAGTCAG AAGATGCAAA GTCTGTTCAT TTTGAATCCA GCACCTCAGA GTCCTCAGTG
    CTATCAAGTA GTAGTCCAGA GAGTACCTTG GTGAAACCAG AGCCGAATGG CATAACCATC
    CCTGGTATCT CTTCAGATGT GCCAGAGAGT GCCCACAAAA CTACTGCCTC AGAGGCAAAG
    TCAGACACTG GGCAGCCTAC CAAGGTTGGA CGTTTTCAGG TGACAACTAC AGCAAACAAA
    GTGGGTCGTT TCTCTGTATC AAAAACTGAG GACAAGATCA CTGACACAAA GAAAGAAGGA
    CCAGTGGCAT CTCCTCCTTT TATGGATTTG GAACAAGCTG TTCTTCCTGC TGTGATACCA
    AAGAAAGAGA AGCCTGAACT GTCAGAGCCT TCACATCTAA ATGGGCCGTC TTCTGACCCG
    GAGGCCGCTT TTTTAAGTAG GGATGTGGAT GATGGTTCCG GTAGTCCACA CTCGCCCCAT
    CAGCTGAGCT CAAAGAGCCT TCCTAGCCAG AATCTAAGTC AAAGCCTTAG TAATTCATTT
    AACTCCTCTT ACATGAGTAG CGACAATGAG TCAGATATCG AAGATGAAGA CTTAAAGTTA
    GAGCTGCGAC GACTACGAGA TAAACATCTC AAAGAGATTC AGGACCTGCA GAGTCGCCAG
    AAGCATGAAA TTGAATCTTT GTATACCAAA CTGGGCAAGG TGCCCCCTGC TGTTATTATT
    CCCCCAGCTG CTCCCCTTTC AGGGAGAAGA CGACGACCCA CTAAAAGCAA AGGCAGCAAA
    TCTAGTCGAA GCAGTTCCTT GGGGAATAAA AGCCCCCAGC TTTCAGGTAA CCTGTCTGGT
    CAGAGTGCAG CTTCAGTCTT GCACCCCCAG CAGACCCTCC ACCCTCCTGG CAACATCCCA
    GAGTCCGGGC AGAATCAGCT GTTACAGCCC CTTAAGCCAT CTCCCTCCAG TGACAACCTC
    TATTCAGCCT TCACCAGTGA TGGTGCCATT TCAGTACCAA GCCTTTCTGC TCCAGGTCAA
    GGAACCAGCA GCACAAACAC TGTTGGGGCA ACAGTGAACA GCCAAGCCGC CCAAGCTCAG
    CCTCCTGCCA TGACGTCCAG CAGGAAGGGC ACATTCACAG ATGACTTGCA CAAGTTGGTA
    GACAATTGGG CCCGAGATGC CATGAATCTC TCAGGCAGGA GAGGAAGCAA AGGGCACATG
    AATTACGAGG GCCCTGGAAT GGCAAGGAAG TTCTCTGCAC CTGGGCAACT GTGCATCTCC
    ATGACCTCGA ACCTGGGTGG CTCTGCCCCC ATCTCTGCAG CATCAGCTAC CTCTCTAGGT
    CACTTCACCA AGTCTATGTG CCCCCCACAG CAGTATGGCT TTCCAGCTAC CCCATTTGGC
    GCTCAATGGA GTGGGACGGG TGGCCCAGCA CCACAGCCAC TTGGCCAGTT CCAACCTGTG
    GGAACTGCCT CCTTGCAGAA TTTCAACATC AGCAATTTGC AGAAATCCAT CAGCAACCCC
    CCAGGCTCCA ACCTGCGGAC CACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71622
    Clone ID Related Accession (Same CDS sequence) XM_011521006.2
    Accession Version XM_011521006.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7065bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase WNK1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521006.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGCAGGGAAT ACAGCAGACA
    GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC
    ACTACTGCAC AGCCAGTGAG TCAGCCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT
    GGAAAACAGC TTCCAGTTTC CCAGCCAGTA CCAACTATCC AAGGCGAACC TCAGATCCCA
    GTTGCGACAC AACCCTCGGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT TCCAGTGGGA
    CAGCCGCTCC CTACTCCCTT GCTCCCTCAG TACCCTGTCT CTCAGATTCC CATATCAACT
    CCTCATGTGT CTACGGCTCA GACAGGTTTC TCATCCCTTC CCATCACAAT GGCAGCTGGC
    ATTACTCAGC CTCTGCTCAC GTTGGCTTCA TCTGCTACAA CAGCTGCGAT CCCGGGGGTA
    TCAACTGTGG TTCCTAGTCA GCTTCCAACC CTTCTGCAGC CTGTGACTCA GCTGCCAAGT
    CAGGTTCACC CACAGCTCCT ACAACCAGCA GTTCAGTCCA TGGGAATACC AGCTAACCTT
    GGACAAGCTG CTGAGGTTCC ACTTTCCTCT GGAGATGTTC TGTACCAGGG CTTCCCACCT
    CGACTGCCAC CACAGTACCC AGGAGATTCA AATATTGCTC CCTCTTCCAA CGTGGCTTCT
    GTTTGCATCC ATTCTACAGT CCTATCCCCT CCCATGCCGA CAGAAGTACT GGCTACACCT
    GGGTACTTTC CCACAGTGGT GCAGCCTTAT GTGGAATCAA ATCTTTTAGT TCCTATGGGT
    GGTGTAGGAG GACAGGTTCA AGTGTCCCAG CCAGGAGGGA GTTTAGCACA AGCCCCCACT
    ACATCCTCCC AGCAAGCAGT TTTGGAGAGT ACTCAGGGAG TCTCTCAGGT TGCTCCTGCA
    GAGCCAGTTG CAGTAGCACA GACCCAAGCT ACCCAGCCGA CCACTTTGGC TTCCTCTGTA
    GACAGTGCAC ATTCAGATGT TGCTTCAGGT ATGAGTGATG GCAATGAGAA CGTCCCATCT
    TCCAGTGGAA GGCATGAAGG AAGAACTACA AAACGGCATT ACCGAAAATC TGTAAGGAGT
    CGCTCTCGAC ATGAAAAAAC TTCACGCCCA AAATTAAGAA TTTTGAATGT TTCAAATAAA
    GGAGACCGAG TAGTAGAATG TCAATTAGAG ACTCATAATA GGAAAATGGT TACATTCAAA
    TTTGACCTAG ATGGTGACAA CCCCGAGGAG ATAGCAACAA TTATGGTGAA CAATGACTTT
    ATTCTAGCAA TAGAGAGAGA GTCGTTTGTG GATCAAGTGC GAGAAATTAT TGAAAAAGCT
    GATGAAATGC TCAGTGAGGA TGTCAGTGTG GAACCAGAGG GTGATCAGGG ATTGGAGAGT
    CTACAAGGAA AGGATGACTA TGGCTTTTCA GGTTCTCAGA AATTGGAAGG AGAGTTCAAA
    CAACCAATTC CTGCGTCTTC CATGCCACAG CAAATAGGCA TTCCTACCAG TTCTTTAACT
    CAAGTTGTTC ATTCTGCGGG AAGGCGGTTT ATAGTGAGTC CTGTGCCAGA AAGCCGATTA
    CGAGAATCAA AAGTTTTCCC CAGTGAAATA ACAGATACAG TTGCTGCCTC TACAGCTCAG
    AGCCCTGGAA TGAACTTGTC TCACTCTGCA TCATCCCTTA GTCTACAACA GGCCTTTTCT
    GAACTTAGAC GTGCCCAAAT GACAGAAGGA CCCAACACAG CACCTCCAAA CTTTAGTCAT
    ACAGGACCAA CATTTCCAGT AGTACCTCCT TTCTTAAGTA GCATTGCTGG AGTCCCAACC
    ACAGCAGCAG CCACAGCACC AGTCCCTGCA ACAAGCAGCC CTCCTAATGA CATTTCCACA
    TCAGTAATTC AGTCTGAGGT TACAGTGCCC ACTGAAGAGG GGATTGCTGG AGTTGCCACC
    AGCACAGGTG TGGTAACTTC AGGTGGTCTC CCCATACCAC CTGTGTCTGA ATCACCAGTA
    CTTTCCAGCG TAGTTTCAAG TATCACAATA CCTGCAGTTG TCTCAATATC TACTACATCC
    CCGTCACTTC AAGTCCCCAC ATCCACATCT GAGATCGTTG TTTCTAGTAC AGCACTGTAT
    CCTTCAGTAA CAGTTTCAGC AACTTCAGCC TCTGCAGGGG GCAGTACTGC TACCCCAGGT
    CCTAAGCCTC CAGCTGTAGT ATCTCAGCAG GCAGCAGGCA GCACTACTGT GGGAGCCACA
    TTAACATCAG TTTCTACCAC CACTTCATTC CCAAGCACAG CTTCACAGCT GTGCATTCAG
    CTTAGCAGCA GTACTTCTAC TCCTACTTTA GCTGAAACCG TGGTAGTTAG CGCACACTCA
    CTAGATAAGA CATCTCATAG CAGTACAACT GGATTGGCTT TCTCCCTCTC TGCACCATCT
    TCCTCTTCCT CTCCTGGAGC AGGAGTGTCT AGTTATATTT CTCAGCCTGG TGGGCTGCAT
    CCTTTGGTCA TTCCATCAGT GATAGCTTCT ACTCCTATTC TTCCCCAAGC AGCAGGACCT
    ACTTCTACAC CTTTATTACC CCAAGTACCT AGTATCCCAC CCTTGGTACA GCCTGTTGCC
    AATGTGCCTG CTGTACAGCA GACACTAATT CATAGTCAGC CTCAACCAGC TTTGCTTCCC
    AACCAGCCCC ATACTCATTG TCCTGAAGTA GATTCTGATA CACAACCCAA AGCTCCTGGA
    ATTGATGACA TAAAGACTCT AGAAGAAAAG CTGCGGTCTC TGTTCAGTGA ACACAGCTCA
    TCTGGAGCTC AGCATGCCTC TGTCTCACTG GAGACCTCAC TAGTCATAGA GAGCACTGTC
    ACACCAGGCA TCCCAACTAC TGCTGTTGCA CCAAGCAAAC TCCTGACTTC TACCACAAGT
    ACTTGCTTAC CACCAACCAA TTTACCACTA GGAACAGTTG CTTTGCCAGT TACACCAGTG
    GTCACACCTG GGCAAGTTTC TACCCCAGTC AGCACTACTA CATCAGGAGT GAAACCTGGA
    ACTGCTCCCT CCAAGCCACC TCTAACTAAG GCTCCGGTGC TGCCAGTGGG TACTGAACTT
    CCAGCAGGTA CTCTACCCAG CGAGCAGCTG CCACCTTTTC CAGGACCTTC TCTAACCCAG
    TCCCAGCAAC CTCTAGAGGA TCTTGATGCT CAATTGAGAA GAACACTTAG TCCAGAGATG
    ATCACAGTGA CTTCTGCGGT TGGTCCTGTG TCCATGGCGG CTCCAACAGC AATCACAGAA
    GCAGGAACAC AGCCTCAGAA GGGTGTTTCT CAAGTCAAAG AAGGCCCTGT CCTAGCAACT
    AGTTCAGGAG CTGGTGTTTT TAAGATGGGA CGATTTCAGG TTTCTGTTGC AGCAGACGGT
    GCCCAGAAAG AGGGTAAAAA TAAGTCAGAA GATGCAAAGT CTGTTCATTT TGAATCCAGC
    ACCTCAGAGT CCTCAGTGCT ATCAAGTAGT AGTCCAGAGA GTACCTTGGT GAAACCAGAG
    CCGAATGGCA TAACCATCCC TGGTATCTCT TCAGATGTGC CAGAGAGTGC CCACAAAACT
    ACTGCCTCAG AGGCAAAGTC AGACACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG
    ACAACTACAG CAAACAAAGT GGGTCGTTTC TCTGTATCAA AAACTGAGGA CAAGATCACT
    GACACAAAGA AAGAAGGACC AGTGGCATCT CCTCCTTTTA TGGATTTGGA ACAAGCTGTT
    CTTCCTGCTG TGATACCAAA GAAAGAGAAG CCTGAACTGT CAGAGCCTTC ACATCTAAAT
    GGGCCGTCTT CTGACCCGGA GGCCGCTTTT TTAAGTAGGG ATGTGGATGA TGGTTCCGGT
    AGTCCACACT CGCCCCATCA GCTGAGCTCA AAGAGCCTTC CTAGCCAGAA TCTAAGTCAA
    AGCCTTAGTA ATTCATTTAA CTCCTCTTAC ATGAGTAGCG ACAATGAGTC AGATATCGAA
    GATGAAGACT TAAAGTTAGA GCTGCGACGA CTACGAGATA AACATCTCAA AGAGATTCAG
    GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGCAAGGTG
    CCCCCTGCTG TTATTATTCC CCCAGCTGCT CCCCTTTCAG GGAGAAGACG ACGACCCACT
    AAAAGCAAAG GCAGCAAATC TAGTCGAAGC AGTTCCTTGG GGAATAAAAG CCCCCAGCTT
    TCAGGTAACC TGTCTGGTCA GAGTGCAGCT TCAGTCTTGC ACCCCCAGCA GACCCTCCAC
    CCTCCTGGCA ACATCCCAGA GTCCGGGCAG AATCAGCTGT TACAGCCCCT TAAGCCATCT
    CCCTCCAGTG ACAACCTCTA TTCAGCCTTC ACCAGTGATG GTGCCATTTC AGTACCAAGC
    CTTTCTGCTC CAGGTCAAGG AACCAGCAGC ACAAACACTG TTGGGGCAAC AGTGAACAGC
    CAAGCCGCCC AAGCTCAGCC TCCTGCCATG ACGTCCAGCA GGAAGGGCAC ATTCACAGAT
    GACTTGCACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGGAGA
    GGAAGCAAAG GGCACATGAA TTACGAGGGC CCTGGAATGG CAAGGAAGTT CTCTGCACCT
    GGGCAACTGT GCATCTCCAT GACCTCGAAC CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA
    TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTATGGCTTT
    CCAGCTACCC CATTTGGCGC TCAATGGAGT GGGACGGGTG GCCCAGCACC ACAGCCACTT
    GGCCAGTTCC AACCTGTGGG AACTGCCTCC TTGCAGAATT TCAACATCAG CAATTTGCAG
    AAATCCATCA GCAACCCCCC AGGCTCCAAC CTGCGGACCA CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011519308.1
    CDS969..8033
    Translation

    Target ORF information:

    RefSeq Version XM_011521006.2
    Organism Homo sapiens(human)
    Definition Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011521006.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGCAGGGAAT ACAGCAGACA
    GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC
    ACTACTGCAC AGCCAGTGAG TCAGCCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT
    GGAAAACAGC TTCCAGTTTC CCAGCCAGTA CCAACTATCC AAGGCGAACC TCAGATCCCA
    GTTGCGACAC AACCCTCGGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT TCCAGTGGGA
    CAGCCGCTCC CTACTCCCTT GCTCCCTCAG TACCCTGTCT CTCAGATTCC CATATCAACT
    CCTCATGTGT CTACGGCTCA GACAGGTTTC TCATCCCTTC CCATCACAAT GGCAGCTGGC
    ATTACTCAGC CTCTGCTCAC GTTGGCTTCA TCTGCTACAA CAGCTGCGAT CCCGGGGGTA
    TCAACTGTGG TTCCTAGTCA GCTTCCAACC CTTCTGCAGC CTGTGACTCA GCTGCCAAGT
    CAGGTTCACC CACAGCTCCT ACAACCAGCA GTTCAGTCCA TGGGAATACC AGCTAACCTT
    GGACAAGCTG CTGAGGTTCC ACTTTCCTCT GGAGATGTTC TGTACCAGGG CTTCCCACCT
    CGACTGCCAC CACAGTACCC AGGAGATTCA AATATTGCTC CCTCTTCCAA CGTGGCTTCT
    GTTTGCATCC ATTCTACAGT CCTATCCCCT CCCATGCCGA CAGAAGTACT GGCTACACCT
    GGGTACTTTC CCACAGTGGT GCAGCCTTAT GTGGAATCAA ATCTTTTAGT TCCTATGGGT
    GGTGTAGGAG GACAGGTTCA AGTGTCCCAG CCAGGAGGGA GTTTAGCACA AGCCCCCACT
    ACATCCTCCC AGCAAGCAGT TTTGGAGAGT ACTCAGGGAG TCTCTCAGGT TGCTCCTGCA
    GAGCCAGTTG CAGTAGCACA GACCCAAGCT ACCCAGCCGA CCACTTTGGC TTCCTCTGTA
    GACAGTGCAC ATTCAGATGT TGCTTCAGGT ATGAGTGATG GCAATGAGAA CGTCCCATCT
    TCCAGTGGAA GGCATGAAGG AAGAACTACA AAACGGCATT ACCGAAAATC TGTAAGGAGT
    CGCTCTCGAC ATGAAAAAAC TTCACGCCCA AAATTAAGAA TTTTGAATGT TTCAAATAAA
    GGAGACCGAG TAGTAGAATG TCAATTAGAG ACTCATAATA GGAAAATGGT TACATTCAAA
    TTTGACCTAG ATGGTGACAA CCCCGAGGAG ATAGCAACAA TTATGGTGAA CAATGACTTT
    ATTCTAGCAA TAGAGAGAGA GTCGTTTGTG GATCAAGTGC GAGAAATTAT TGAAAAAGCT
    GATGAAATGC TCAGTGAGGA TGTCAGTGTG GAACCAGAGG GTGATCAGGG ATTGGAGAGT
    CTACAAGGAA AGGATGACTA TGGCTTTTCA GGTTCTCAGA AATTGGAAGG AGAGTTCAAA
    CAACCAATTC CTGCGTCTTC CATGCCACAG CAAATAGGCA TTCCTACCAG TTCTTTAACT
    CAAGTTGTTC ATTCTGCGGG AAGGCGGTTT ATAGTGAGTC CTGTGCCAGA AAGCCGATTA
    CGAGAATCAA AAGTTTTCCC CAGTGAAATA ACAGATACAG TTGCTGCCTC TACAGCTCAG
    AGCCCTGGAA TGAACTTGTC TCACTCTGCA TCATCCCTTA GTCTACAACA GGCCTTTTCT
    GAACTTAGAC GTGCCCAAAT GACAGAAGGA CCCAACACAG CACCTCCAAA CTTTAGTCAT
    ACAGGACCAA CATTTCCAGT AGTACCTCCT TTCTTAAGTA GCATTGCTGG AGTCCCAACC
    ACAGCAGCAG CCACAGCACC AGTCCCTGCA ACAAGCAGCC CTCCTAATGA CATTTCCACA
    TCAGTAATTC AGTCTGAGGT TACAGTGCCC ACTGAAGAGG GGATTGCTGG AGTTGCCACC
    AGCACAGGTG TGGTAACTTC AGGTGGTCTC CCCATACCAC CTGTGTCTGA ATCACCAGTA
    CTTTCCAGCG TAGTTTCAAG TATCACAATA CCTGCAGTTG TCTCAATATC TACTACATCC
    CCGTCACTTC AAGTCCCCAC ATCCACATCT GAGATCGTTG TTTCTAGTAC AGCACTGTAT
    CCTTCAGTAA CAGTTTCAGC AACTTCAGCC TCTGCAGGGG GCAGTACTGC TACCCCAGGT
    CCTAAGCCTC CAGCTGTAGT ATCTCAGCAG GCAGCAGGCA GCACTACTGT GGGAGCCACA
    TTAACATCAG TTTCTACCAC CACTTCATTC CCAAGCACAG CTTCACAGCT GTGCATTCAG
    CTTAGCAGCA GTACTTCTAC TCCTACTTTA GCTGAAACCG TGGTAGTTAG CGCACACTCA
    CTAGATAAGA CATCTCATAG CAGTACAACT GGATTGGCTT TCTCCCTCTC TGCACCATCT
    TCCTCTTCCT CTCCTGGAGC AGGAGTGTCT AGTTATATTT CTCAGCCTGG TGGGCTGCAT
    CCTTTGGTCA TTCCATCAGT GATAGCTTCT ACTCCTATTC TTCCCCAAGC AGCAGGACCT
    ACTTCTACAC CTTTATTACC CCAAGTACCT AGTATCCCAC CCTTGGTACA GCCTGTTGCC
    AATGTGCCTG CTGTACAGCA GACACTAATT CATAGTCAGC CTCAACCAGC TTTGCTTCCC
    AACCAGCCCC ATACTCATTG TCCTGAAGTA GATTCTGATA CACAACCCAA AGCTCCTGGA
    ATTGATGACA TAAAGACTCT AGAAGAAAAG CTGCGGTCTC TGTTCAGTGA ACACAGCTCA
    TCTGGAGCTC AGCATGCCTC TGTCTCACTG GAGACCTCAC TAGTCATAGA GAGCACTGTC
    ACACCAGGCA TCCCAACTAC TGCTGTTGCA CCAAGCAAAC TCCTGACTTC TACCACAAGT
    ACTTGCTTAC CACCAACCAA TTTACCACTA GGAACAGTTG CTTTGCCAGT TACACCAGTG
    GTCACACCTG GGCAAGTTTC TACCCCAGTC AGCACTACTA CATCAGGAGT GAAACCTGGA
    ACTGCTCCCT CCAAGCCACC TCTAACTAAG GCTCCGGTGC TGCCAGTGGG TACTGAACTT
    CCAGCAGGTA CTCTACCCAG CGAGCAGCTG CCACCTTTTC CAGGACCTTC TCTAACCCAG
    TCCCAGCAAC CTCTAGAGGA TCTTGATGCT CAATTGAGAA GAACACTTAG TCCAGAGATG
    ATCACAGTGA CTTCTGCGGT TGGTCCTGTG TCCATGGCGG CTCCAACAGC AATCACAGAA
    GCAGGAACAC AGCCTCAGAA GGGTGTTTCT CAAGTCAAAG AAGGCCCTGT CCTAGCAACT
    AGTTCAGGAG CTGGTGTTTT TAAGATGGGA CGATTTCAGG TTTCTGTTGC AGCAGACGGT
    GCCCAGAAAG AGGGTAAAAA TAAGTCAGAA GATGCAAAGT CTGTTCATTT TGAATCCAGC
    ACCTCAGAGT CCTCAGTGCT ATCAAGTAGT AGTCCAGAGA GTACCTTGGT GAAACCAGAG
    CCGAATGGCA TAACCATCCC TGGTATCTCT TCAGATGTGC CAGAGAGTGC CCACAAAACT
    ACTGCCTCAG AGGCAAAGTC AGACACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG
    ACAACTACAG CAAACAAAGT GGGTCGTTTC TCTGTATCAA AAACTGAGGA CAAGATCACT
    GACACAAAGA AAGAAGGACC AGTGGCATCT CCTCCTTTTA TGGATTTGGA ACAAGCTGTT
    CTTCCTGCTG TGATACCAAA GAAAGAGAAG CCTGAACTGT CAGAGCCTTC ACATCTAAAT
    GGGCCGTCTT CTGACCCGGA GGCCGCTTTT TTAAGTAGGG ATGTGGATGA TGGTTCCGGT
    AGTCCACACT CGCCCCATCA GCTGAGCTCA AAGAGCCTTC CTAGCCAGAA TCTAAGTCAA
    AGCCTTAGTA ATTCATTTAA CTCCTCTTAC ATGAGTAGCG ACAATGAGTC AGATATCGAA
    GATGAAGACT TAAAGTTAGA GCTGCGACGA CTACGAGATA AACATCTCAA AGAGATTCAG
    GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGCAAGGTG
    CCCCCTGCTG TTATTATTCC CCCAGCTGCT CCCCTTTCAG GGAGAAGACG ACGACCCACT
    AAAAGCAAAG GCAGCAAATC TAGTCGAAGC AGTTCCTTGG GGAATAAAAG CCCCCAGCTT
    TCAGGTAACC TGTCTGGTCA GAGTGCAGCT TCAGTCTTGC ACCCCCAGCA GACCCTCCAC
    CCTCCTGGCA ACATCCCAGA GTCCGGGCAG AATCAGCTGT TACAGCCCCT TAAGCCATCT
    CCCTCCAGTG ACAACCTCTA TTCAGCCTTC ACCAGTGATG GTGCCATTTC AGTACCAAGC
    CTTTCTGCTC CAGGTCAAGG AACCAGCAGC ACAAACACTG TTGGGGCAAC AGTGAACAGC
    CAAGCCGCCC AAGCTCAGCC TCCTGCCATG ACGTCCAGCA GGAAGGGCAC ATTCACAGAT
    GACTTGCACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGGAGA
    GGAAGCAAAG GGCACATGAA TTACGAGGGC CCTGGAATGG CAAGGAAGTT CTCTGCACCT
    GGGCAACTGT GCATCTCCAT GACCTCGAAC CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA
    TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTATGGCTTT
    CCAGCTACCC CATTTGGCGC TCAATGGAGT GGGACGGGTG GCCCAGCACC ACAGCCACTT
    GGCCAGTTCC AACCTGTGGG AACTGCCTCC TTGCAGAATT TCAACATCAG CAATTTGCAG
    AAATCCATCA GCAACCCCCC AGGCTCCAAC CTGCGGACCA CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71623
    Clone ID Related Accession (Same CDS sequence) XM_011521007.2
    Accession Version XM_011521007.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7062bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase WNK1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521007.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGGGAATACA GCAGACAGCC
    CCTCCTCAAC AGACAGTGCA GTATTCACTT TCACAGACAT CAACCTCCAG TGAGGCCACT
    ACTGCACAGC CAGTGAGTCA GCCTCAAGCT CCACAAGTCT TGCCTCAAGT ATCAGCTGGA
    AAACAGCTTC CAGTTTCCCA GCCAGTACCA ACTATCCAAG GCGAACCTCA GATCCCAGTT
    GCGACACAAC CCTCGGTTGT TCCAGTCCAC TCTGGTGCTC ATTTCCTTCC AGTGGGACAG
    CCGCTCCCTA CTCCCTTGCT CCCTCAGTAC CCTGTCTCTC AGATTCCCAT ATCAACTCCT
    CATGTGTCTA CGGCTCAGAC AGGTTTCTCA TCCCTTCCCA TCACAATGGC AGCTGGCATT
    ACTCAGCCTC TGCTCACGTT GGCTTCATCT GCTACAACAG CTGCGATCCC GGGGGTATCA
    ACTGTGGTTC CTAGTCAGCT TCCAACCCTT CTGCAGCCTG TGACTCAGCT GCCAAGTCAG
    GTTCACCCAC AGCTCCTACA ACCAGCAGTT CAGTCCATGG GAATACCAGC TAACCTTGGA
    CAAGCTGCTG AGGTTCCACT TTCCTCTGGA GATGTTCTGT ACCAGGGCTT CCCACCTCGA
    CTGCCACCAC AGTACCCAGG AGATTCAAAT ATTGCTCCCT CTTCCAACGT GGCTTCTGTT
    TGCATCCATT CTACAGTCCT ATCCCCTCCC ATGCCGACAG AAGTACTGGC TACACCTGGG
    TACTTTCCCA CAGTGGTGCA GCCTTATGTG GAATCAAATC TTTTAGTTCC TATGGGTGGT
    GTAGGAGGAC AGGTTCAAGT GTCCCAGCCA GGAGGGAGTT TAGCACAAGC CCCCACTACA
    TCCTCCCAGC AAGCAGTTTT GGAGAGTACT CAGGGAGTCT CTCAGGTTGC TCCTGCAGAG
    CCAGTTGCAG TAGCACAGAC CCAAGCTACC CAGCCGACCA CTTTGGCTTC CTCTGTAGAC
    AGTGCACATT CAGATGTTGC TTCAGGTATG AGTGATGGCA ATGAGAACGT CCCATCTTCC
    AGTGGAAGGC ATGAAGGAAG AACTACAAAA CGGCATTACC GAAAATCTGT AAGGAGTCGC
    TCTCGACATG AAAAAACTTC ACGCCCAAAA TTAAGAATTT TGAATGTTTC AAATAAAGGA
    GACCGAGTAG TAGAATGTCA ATTAGAGACT CATAATAGGA AAATGGTTAC ATTCAAATTT
    GACCTAGATG GTGACAACCC CGAGGAGATA GCAACAATTA TGGTGAACAA TGACTTTATT
    CTAGCAATAG AGAGAGAGTC GTTTGTGGAT CAAGTGCGAG AAATTATTGA AAAAGCTGAT
    GAAATGCTCA GTGAGGATGT CAGTGTGGAA CCAGAGGGTG ATCAGGGATT GGAGAGTCTA
    CAAGGAAAGG ATGACTATGG CTTTTCAGGT TCTCAGAAAT TGGAAGGAGA GTTCAAACAA
    CCAATTCCTG CGTCTTCCAT GCCACAGCAA ATAGGCATTC CTACCAGTTC TTTAACTCAA
    GTTGTTCATT CTGCGGGAAG GCGGTTTATA GTGAGTCCTG TGCCAGAAAG CCGATTACGA
    GAATCAAAAG TTTTCCCCAG TGAAATAACA GATACAGTTG CTGCCTCTAC AGCTCAGAGC
    CCTGGAATGA ACTTGTCTCA CTCTGCATCA TCCCTTAGTC TACAACAGGC CTTTTCTGAA
    CTTAGACGTG CCCAAATGAC AGAAGGACCC AACACAGCAC CTCCAAACTT TAGTCATACA
    GGACCAACAT TTCCAGTAGT ACCTCCTTTC TTAAGTAGCA TTGCTGGAGT CCCAACCACA
    GCAGCAGCCA CAGCACCAGT CCCTGCAACA AGCAGCCCTC CTAATGACAT TTCCACATCA
    GTAATTCAGT CTGAGGTTAC AGTGCCCACT GAAGAGGGGA TTGCTGGAGT TGCCACCAGC
    ACAGGTGTGG TAACTTCAGG TGGTCTCCCC ATACCACCTG TGTCTGAATC ACCAGTACTT
    TCCAGCGTAG TTTCAAGTAT CACAATACCT GCAGTTGTCT CAATATCTAC TACATCCCCG
    TCACTTCAAG TCCCCACATC CACATCTGAG ATCGTTGTTT CTAGTACAGC ACTGTATCCT
    TCAGTAACAG TTTCAGCAAC TTCAGCCTCT GCAGGGGGCA GTACTGCTAC CCCAGGTCCT
    AAGCCTCCAG CTGTAGTATC TCAGCAGGCA GCAGGCAGCA CTACTGTGGG AGCCACATTA
    ACATCAGTTT CTACCACCAC TTCATTCCCA AGCACAGCTT CACAGCTGTG CATTCAGCTT
    AGCAGCAGTA CTTCTACTCC TACTTTAGCT GAAACCGTGG TAGTTAGCGC ACACTCACTA
    GATAAGACAT CTCATAGCAG TACAACTGGA TTGGCTTTCT CCCTCTCTGC ACCATCTTCC
    TCTTCCTCTC CTGGAGCAGG AGTGTCTAGT TATATTTCTC AGCCTGGTGG GCTGCATCCT
    TTGGTCATTC CATCAGTGAT AGCTTCTACT CCTATTCTTC CCCAAGCAGC AGGACCTACT
    TCTACACCTT TATTACCCCA AGTACCTAGT ATCCCACCCT TGGTACAGCC TGTTGCCAAT
    GTGCCTGCTG TACAGCAGAC ACTAATTCAT AGTCAGCCTC AACCAGCTTT GCTTCCCAAC
    CAGCCCCATA CTCATTGTCC TGAAGTAGAT TCTGATACAC AACCCAAAGC TCCTGGAATT
    GATGACATAA AGACTCTAGA AGAAAAGCTG CGGTCTCTGT TCAGTGAACA CAGCTCATCT
    GGAGCTCAGC ATGCCTCTGT CTCACTGGAG ACCTCACTAG TCATAGAGAG CACTGTCACA
    CCAGGCATCC CAACTACTGC TGTTGCACCA AGCAAACTCC TGACTTCTAC CACAAGTACT
    TGCTTACCAC CAACCAATTT ACCACTAGGA ACAGTTGCTT TGCCAGTTAC ACCAGTGGTC
    ACACCTGGGC AAGTTTCTAC CCCAGTCAGC ACTACTACAT CAGGAGTGAA ACCTGGAACT
    GCTCCCTCCA AGCCACCTCT AACTAAGGCT CCGGTGCTGC CAGTGGGTAC TGAACTTCCA
    GCAGGTACTC TACCCAGCGA GCAGCTGCCA CCTTTTCCAG GACCTTCTCT AACCCAGTCC
    CAGCAACCTC TAGAGGATCT TGATGCTCAA TTGAGAAGAA CACTTAGTCC AGAGATGATC
    ACAGTGACTT CTGCGGTTGG TCCTGTGTCC ATGGCGGCTC CAACAGCAAT CACAGAAGCA
    GGAACACAGC CTCAGAAGGG TGTTTCTCAA GTCAAAGAAG GCCCTGTCCT AGCAACTAGT
    TCAGGAGCTG GTGTTTTTAA GATGGGACGA TTTCAGGTTT CTGTTGCAGC AGACGGTGCC
    CAGAAAGAGG GTAAAAATAA GTCAGAAGAT GCAAAGTCTG TTCATTTTGA ATCCAGCACC
    TCAGAGTCCT CAGTGCTATC AAGTAGTAGT CCAGAGAGTA CCTTGGTGAA ACCAGAGCCG
    AATGGCATAA CCATCCCTGG TATCTCTTCA GATGTGCCAG AGAGTGCCCA CAAAACTACT
    GCCTCAGAGG CAAAGTCAGA CACTGGGCAG CCTACCAAGG TTGGACGTTT TCAGGTGACA
    ACTACAGCAA ACAAAGTGGG TCGTTTCTCT GTATCAAAAA CTGAGGACAA GATCACTGAC
    ACAAAGAAAG AAGGACCAGT GGCATCTCCT CCTTTTATGG ATTTGGAACA AGCTGTTCTT
    CCTGCTGTGA TACCAAAGAA AGAGAAGCCT GAACTGTCAG AGCCTTCACA TCTAAATGGG
    CCGTCTTCTG ACCCGGAGGC CGCTTTTTTA AGTAGGGATG TGGATGATGG TTCCGGTAGT
    CCACACTCGC CCCATCAGCT GAGCTCAAAG AGCCTTCCTA GCCAGAATCT AAGTCAAAGC
    CTTAGTAATT CATTTAACTC CTCTTACATG AGTAGCGACA ATGAGTCAGA TATCGAAGAT
    GAAGACTTAA AGTTAGAGCT GCGACGACTA CGAGATAAAC ATCTCAAAGA GATTCAGGAC
    CTGCAGAGTC GCCAGAAGCA TGAAATTGAA TCTTTGTATA CCAAACTGGG CAAGGTGCCC
    CCTGCTGTTA TTATTCCCCC AGCTGCTCCC CTTTCAGGGA GAAGACGACG ACCCACTAAA
    AGCAAAGGCA GCAAATCTAG TCGAAGCAGT TCCTTGGGGA ATAAAAGCCC CCAGCTTTCA
    GGTAACCTGT CTGGTCAGAG TGCAGCTTCA GTCTTGCACC CCCAGCAGAC CCTCCACCCT
    CCTGGCAACA TCCCAGAGTC CGGGCAGAAT CAGCTGTTAC AGCCCCTTAA GCCATCTCCC
    TCCAGTGACA ACCTCTATTC AGCCTTCACC AGTGATGGTG CCATTTCAGT ACCAAGCCTT
    TCTGCTCCAG GTCAAGGAAC CAGCAGCACA AACACTGTTG GGGCAACAGT GAACAGCCAA
    GCCGCCCAAG CTCAGCCTCC TGCCATGACG TCCAGCAGGA AGGGCACATT CACAGATGAC
    TTGCACAAGT TGGTAGACAA TTGGGCCCGA GATGCCATGA ATCTCTCAGG CAGGAGAGGA
    AGCAAAGGGC ACATGAATTA CGAGGGCCCT GGAATGGCAA GGAAGTTCTC TGCACCTGGG
    CAACTGTGCA TCTCCATGAC CTCGAACCTG GGTGGCTCTG CCCCCATCTC TGCAGCATCA
    GCTACCTCTC TAGGTCACTT CACCAAGTCT ATGTGCCCCC CACAGCAGTA TGGCTTTCCA
    GCTACCCCAT TTGGCGCTCA ATGGAGTGGG ACGGGTGGCC CAGCACCACA GCCACTTGGC
    CAGTTCCAAC CTGTGGGAAC TGCCTCCTTG CAGAATTTCA ACATCAGCAA TTTGCAGAAA
    TCCATCAGCA ACCCCCCAGG CTCCAACCTG CGGACCACTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011519309.1
    CDS969..8030
    Translation

    Target ORF information:

    RefSeq Version XM_011521007.2
    Organism Homo sapiens(human)
    Definition Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011521007.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGGGAATACA GCAGACAGCC
    CCTCCTCAAC AGACAGTGCA GTATTCACTT TCACAGACAT CAACCTCCAG TGAGGCCACT
    ACTGCACAGC CAGTGAGTCA GCCTCAAGCT CCACAAGTCT TGCCTCAAGT ATCAGCTGGA
    AAACAGCTTC CAGTTTCCCA GCCAGTACCA ACTATCCAAG GCGAACCTCA GATCCCAGTT
    GCGACACAAC CCTCGGTTGT TCCAGTCCAC TCTGGTGCTC ATTTCCTTCC AGTGGGACAG
    CCGCTCCCTA CTCCCTTGCT CCCTCAGTAC CCTGTCTCTC AGATTCCCAT ATCAACTCCT
    CATGTGTCTA CGGCTCAGAC AGGTTTCTCA TCCCTTCCCA TCACAATGGC AGCTGGCATT
    ACTCAGCCTC TGCTCACGTT GGCTTCATCT GCTACAACAG CTGCGATCCC GGGGGTATCA
    ACTGTGGTTC CTAGTCAGCT TCCAACCCTT CTGCAGCCTG TGACTCAGCT GCCAAGTCAG
    GTTCACCCAC AGCTCCTACA ACCAGCAGTT CAGTCCATGG GAATACCAGC TAACCTTGGA
    CAAGCTGCTG AGGTTCCACT TTCCTCTGGA GATGTTCTGT ACCAGGGCTT CCCACCTCGA
    CTGCCACCAC AGTACCCAGG AGATTCAAAT ATTGCTCCCT CTTCCAACGT GGCTTCTGTT
    TGCATCCATT CTACAGTCCT ATCCCCTCCC ATGCCGACAG AAGTACTGGC TACACCTGGG
    TACTTTCCCA CAGTGGTGCA GCCTTATGTG GAATCAAATC TTTTAGTTCC TATGGGTGGT
    GTAGGAGGAC AGGTTCAAGT GTCCCAGCCA GGAGGGAGTT TAGCACAAGC CCCCACTACA
    TCCTCCCAGC AAGCAGTTTT GGAGAGTACT CAGGGAGTCT CTCAGGTTGC TCCTGCAGAG
    CCAGTTGCAG TAGCACAGAC CCAAGCTACC CAGCCGACCA CTTTGGCTTC CTCTGTAGAC
    AGTGCACATT CAGATGTTGC TTCAGGTATG AGTGATGGCA ATGAGAACGT CCCATCTTCC
    AGTGGAAGGC ATGAAGGAAG AACTACAAAA CGGCATTACC GAAAATCTGT AAGGAGTCGC
    TCTCGACATG AAAAAACTTC ACGCCCAAAA TTAAGAATTT TGAATGTTTC AAATAAAGGA
    GACCGAGTAG TAGAATGTCA ATTAGAGACT CATAATAGGA AAATGGTTAC ATTCAAATTT
    GACCTAGATG GTGACAACCC CGAGGAGATA GCAACAATTA TGGTGAACAA TGACTTTATT
    CTAGCAATAG AGAGAGAGTC GTTTGTGGAT CAAGTGCGAG AAATTATTGA AAAAGCTGAT
    GAAATGCTCA GTGAGGATGT CAGTGTGGAA CCAGAGGGTG ATCAGGGATT GGAGAGTCTA
    CAAGGAAAGG ATGACTATGG CTTTTCAGGT TCTCAGAAAT TGGAAGGAGA GTTCAAACAA
    CCAATTCCTG CGTCTTCCAT GCCACAGCAA ATAGGCATTC CTACCAGTTC TTTAACTCAA
    GTTGTTCATT CTGCGGGAAG GCGGTTTATA GTGAGTCCTG TGCCAGAAAG CCGATTACGA
    GAATCAAAAG TTTTCCCCAG TGAAATAACA GATACAGTTG CTGCCTCTAC AGCTCAGAGC
    CCTGGAATGA ACTTGTCTCA CTCTGCATCA TCCCTTAGTC TACAACAGGC CTTTTCTGAA
    CTTAGACGTG CCCAAATGAC AGAAGGACCC AACACAGCAC CTCCAAACTT TAGTCATACA
    GGACCAACAT TTCCAGTAGT ACCTCCTTTC TTAAGTAGCA TTGCTGGAGT CCCAACCACA
    GCAGCAGCCA CAGCACCAGT CCCTGCAACA AGCAGCCCTC CTAATGACAT TTCCACATCA
    GTAATTCAGT CTGAGGTTAC AGTGCCCACT GAAGAGGGGA TTGCTGGAGT TGCCACCAGC
    ACAGGTGTGG TAACTTCAGG TGGTCTCCCC ATACCACCTG TGTCTGAATC ACCAGTACTT
    TCCAGCGTAG TTTCAAGTAT CACAATACCT GCAGTTGTCT CAATATCTAC TACATCCCCG
    TCACTTCAAG TCCCCACATC CACATCTGAG ATCGTTGTTT CTAGTACAGC ACTGTATCCT
    TCAGTAACAG TTTCAGCAAC TTCAGCCTCT GCAGGGGGCA GTACTGCTAC CCCAGGTCCT
    AAGCCTCCAG CTGTAGTATC TCAGCAGGCA GCAGGCAGCA CTACTGTGGG AGCCACATTA
    ACATCAGTTT CTACCACCAC TTCATTCCCA AGCACAGCTT CACAGCTGTG CATTCAGCTT
    AGCAGCAGTA CTTCTACTCC TACTTTAGCT GAAACCGTGG TAGTTAGCGC ACACTCACTA
    GATAAGACAT CTCATAGCAG TACAACTGGA TTGGCTTTCT CCCTCTCTGC ACCATCTTCC
    TCTTCCTCTC CTGGAGCAGG AGTGTCTAGT TATATTTCTC AGCCTGGTGG GCTGCATCCT
    TTGGTCATTC CATCAGTGAT AGCTTCTACT CCTATTCTTC CCCAAGCAGC AGGACCTACT
    TCTACACCTT TATTACCCCA AGTACCTAGT ATCCCACCCT TGGTACAGCC TGTTGCCAAT
    GTGCCTGCTG TACAGCAGAC ACTAATTCAT AGTCAGCCTC AACCAGCTTT GCTTCCCAAC
    CAGCCCCATA CTCATTGTCC TGAAGTAGAT TCTGATACAC AACCCAAAGC TCCTGGAATT
    GATGACATAA AGACTCTAGA AGAAAAGCTG CGGTCTCTGT TCAGTGAACA CAGCTCATCT
    GGAGCTCAGC ATGCCTCTGT CTCACTGGAG ACCTCACTAG TCATAGAGAG CACTGTCACA
    CCAGGCATCC CAACTACTGC TGTTGCACCA AGCAAACTCC TGACTTCTAC CACAAGTACT
    TGCTTACCAC CAACCAATTT ACCACTAGGA ACAGTTGCTT TGCCAGTTAC ACCAGTGGTC
    ACACCTGGGC AAGTTTCTAC CCCAGTCAGC ACTACTACAT CAGGAGTGAA ACCTGGAACT
    GCTCCCTCCA AGCCACCTCT AACTAAGGCT CCGGTGCTGC CAGTGGGTAC TGAACTTCCA
    GCAGGTACTC TACCCAGCGA GCAGCTGCCA CCTTTTCCAG GACCTTCTCT AACCCAGTCC
    CAGCAACCTC TAGAGGATCT TGATGCTCAA TTGAGAAGAA CACTTAGTCC AGAGATGATC
    ACAGTGACTT CTGCGGTTGG TCCTGTGTCC ATGGCGGCTC CAACAGCAAT CACAGAAGCA
    GGAACACAGC CTCAGAAGGG TGTTTCTCAA GTCAAAGAAG GCCCTGTCCT AGCAACTAGT
    TCAGGAGCTG GTGTTTTTAA GATGGGACGA TTTCAGGTTT CTGTTGCAGC AGACGGTGCC
    CAGAAAGAGG GTAAAAATAA GTCAGAAGAT GCAAAGTCTG TTCATTTTGA ATCCAGCACC
    TCAGAGTCCT CAGTGCTATC AAGTAGTAGT CCAGAGAGTA CCTTGGTGAA ACCAGAGCCG
    AATGGCATAA CCATCCCTGG TATCTCTTCA GATGTGCCAG AGAGTGCCCA CAAAACTACT
    GCCTCAGAGG CAAAGTCAGA CACTGGGCAG CCTACCAAGG TTGGACGTTT TCAGGTGACA
    ACTACAGCAA ACAAAGTGGG TCGTTTCTCT GTATCAAAAA CTGAGGACAA GATCACTGAC
    ACAAAGAAAG AAGGACCAGT GGCATCTCCT CCTTTTATGG ATTTGGAACA AGCTGTTCTT
    CCTGCTGTGA TACCAAAGAA AGAGAAGCCT GAACTGTCAG AGCCTTCACA TCTAAATGGG
    CCGTCTTCTG ACCCGGAGGC CGCTTTTTTA AGTAGGGATG TGGATGATGG TTCCGGTAGT
    CCACACTCGC CCCATCAGCT GAGCTCAAAG AGCCTTCCTA GCCAGAATCT AAGTCAAAGC
    CTTAGTAATT CATTTAACTC CTCTTACATG AGTAGCGACA ATGAGTCAGA TATCGAAGAT
    GAAGACTTAA AGTTAGAGCT GCGACGACTA CGAGATAAAC ATCTCAAAGA GATTCAGGAC
    CTGCAGAGTC GCCAGAAGCA TGAAATTGAA TCTTTGTATA CCAAACTGGG CAAGGTGCCC
    CCTGCTGTTA TTATTCCCCC AGCTGCTCCC CTTTCAGGGA GAAGACGACG ACCCACTAAA
    AGCAAAGGCA GCAAATCTAG TCGAAGCAGT TCCTTGGGGA ATAAAAGCCC CCAGCTTTCA
    GGTAACCTGT CTGGTCAGAG TGCAGCTTCA GTCTTGCACC CCCAGCAGAC CCTCCACCCT
    CCTGGCAACA TCCCAGAGTC CGGGCAGAAT CAGCTGTTAC AGCCCCTTAA GCCATCTCCC
    TCCAGTGACA ACCTCTATTC AGCCTTCACC AGTGATGGTG CCATTTCAGT ACCAAGCCTT
    TCTGCTCCAG GTCAAGGAAC CAGCAGCACA AACACTGTTG GGGCAACAGT GAACAGCCAA
    GCCGCCCAAG CTCAGCCTCC TGCCATGACG TCCAGCAGGA AGGGCACATT CACAGATGAC
    TTGCACAAGT TGGTAGACAA TTGGGCCCGA GATGCCATGA ATCTCTCAGG CAGGAGAGGA
    AGCAAAGGGC ACATGAATTA CGAGGGCCCT GGAATGGCAA GGAAGTTCTC TGCACCTGGG
    CAACTGTGCA TCTCCATGAC CTCGAACCTG GGTGGCTCTG CCCCCATCTC TGCAGCATCA
    GCTACCTCTC TAGGTCACTT CACCAAGTCT ATGTGCCCCC CACAGCAGTA TGGCTTTCCA
    GCTACCCCAT TTGGCGCTCA ATGGAGTGGG ACGGGTGGCC CAGCACCACA GCCACTTGGC
    CAGTTCCAAC CTGTGGGAAC TGCCTCCTTG CAGAATTTCA ACATCAGCAA TTTGCAGAAA
    TCCATCAGCA ACCCCCCAGG CTCCAACCTG CGGACCACTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71624
    Clone ID Related Accession (Same CDS sequence) XM_011521008.2
    Accession Version XM_011521008.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6327bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase WNK1 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521008.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGCAGGGAAT ACAGCAGACA
    GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC
    ACTACTGCAC AGCCAGTGAG TCAGCCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT
    GGAAAACAGA GTACTCAGGG AGTCTCTCAG GTTGCTCCTG CAGAGCCAGT TGCAGTAGCA
    CAGACCCAAG CTACCCAGCC GACCACTTTG GCTTCCTCTG TAGACAGTGC ACATTCAGAT
    GTTGCTTCAG GTATGAGTGA TGGCAATGAG AACGTCCCAT CTTCCAGTGG AAGGCATGAA
    GGAAGAACTA CAAAACGGCA TTACCGAAAA TCTGTAAGGA GTCGCTCTCG ACATGAAAAA
    ACTTCACGCC CAAAATTAAG AATTTTGAAT GTTTCAAATA AAGGAGACCG AGTAGTAGAA
    TGTCAATTAG AGACTCATAA TAGGAAAATG GTTACATTCA AATTTGACCT AGATGGTGAC
    AACCCCGAGG AGATAGCAAC AATTATGGTG AACAATGACT TTATTCTAGC AATAGAGAGA
    GAGTCGTTTG TGGATCAAGT GCGAGAAATT ATTGAAAAAG CTGATGAAAT GCTCAGTGAG
    GATGTCAGTG TGGAACCAGA GGGTGATCAG GGATTGGAGA GTCTACAAGG AAAGGATGAC
    TATGGCTTTT CAGGTTCTCA GAAATTGGAA GGAGAGTTCA AACAACCAAT TCCTGCGTCT
    TCCATGCCAC AGCAAATAGG CATTCCTACC AGTTCTTTAA CTCAAGTTGT TCATTCTGCG
    GGAAGGCGGT TTATAGTGAG TCCTGTGCCA GAAAGCCGAT TACGAGAATC AAAAGTTTTC
    CCCAGTGAAA TAACAGATAC AGTTGCTGCC TCTACAGCTC AGAGCCCTGG AATGAACTTG
    TCTCACTCTG CATCATCCCT TAGTCTACAA CAGGCCTTTT CTGAACTTAG ACGTGCCCAA
    ATGACAGAAG GACCCAACAC AGCACCTCCA AACTTTAGTC ATACAGGACC AACATTTCCA
    GTAGTACCTC CTTTCTTAAG TAGCATTGCT GGAGTCCCAA CCACAGCAGC AGCCACAGCA
    CCAGTCCCTG CAACAAGCAG CCCTCCTAAT GACATTTCCA CATCAGTAAT TCAGTCTGAG
    GTTACAGTGC CCACTGAAGA GGGGATTGCT GGAGTTGCCA CCAGCACAGG TGTGGTAACT
    TCAGGTGGTC TCCCCATACC ACCTGTGTCT GAATCACCAG TACTTTCCAG CGTAGTTTCA
    AGTATCACAA TACCTGCAGT TGTCTCAATA TCTACTACAT CCCCGTCACT TCAAGTCCCC
    ACATCCACAT CTGAGATCGT TGTTTCTAGT ACAGCACTGT ATCCTTCAGT AACAGTTTCA
    GCAACTTCAG CCTCTGCAGG GGGCAGTACT GCTACCCCAG GTCCTAAGCC TCCAGCTGTA
    GTATCTCAGC AGGCAGCAGG CAGCACTACT GTGGGAGCCA CATTAACATC AGTTTCTACC
    ACCACTTCAT TCCCAAGCAC AGCTTCACAG CTGTGCATTC AGCTTAGCAG CAGTACTTCT
    ACTCCTACTT TAGCTGAAAC CGTGGTAGTT AGCGCACACT CACTAGATAA GACATCTCAT
    AGCAGTACAA CTGGATTGGC TTTCTCCCTC TCTGCACCAT CTTCCTCTTC CTCTCCTGGA
    GCAGGAGTGT CTAGTTATAT TTCTCAGCCT GGTGGGCTGC ATCCTTTGGT CATTCCATCA
    GTGATAGCTT CTACTCCTAT TCTTCCCCAA GCAGCAGGAC CTACTTCTAC ACCTTTATTA
    CCCCAAGTAC CTAGTATCCC ACCCTTGGTA CAGCCTGTTG CCAATGTGCC TGCTGTACAG
    CAGACACTAA TTCATAGTCA GCCTCAACCA GCTTTGCTTC CCAACCAGCC CCATACTCAT
    TGTCCTGAAG TAGATTCTGA TACACAACCC AAAGCTCCTG GAATTGATGA CATAAAGACT
    CTAGAAGAAA AGCTGCGGTC TCTGTTCAGT GAACACAGCT CATCTGGAGC TCAGCATGCC
    TCTGTCTCAC TGGAGACCTC ACTAGTCATA GAGAGCACTG TCACACCAGG CATCCCAACT
    ACTGCTGTTG CACCAAGCAA ACTCCTGACT TCTACCACAA GTACTTGCTT ACCACCAACC
    AATTTACCAC TAGGAACAGT TGCTTTGCCA GTTACACCAG TGGTCACACC TGGGCAAGTT
    TCTACCCCAG TCAGCACTAC TACATCAGGA GTGAAACCTG GAACTGCTCC CTCCAAGCCA
    CCTCTAACTA AGGCTCCGGT GCTGCCAGTG GGTACTGAAC TTCCAGCAGG TACTCTACCC
    AGCGAGCAGC TGCCACCTTT TCCAGGACCT TCTCTAACCC AGTCCCAGCA ACCTCTAGAG
    GATCTTGATG CTCAATTGAG AAGAACACTT AGTCCAGAGA TGATCACAGT GACTTCTGCG
    GTTGGTCCTG TGTCCATGGC GGCTCCAACA GCAATCACAG AAGCAGGAAC ACAGCCTCAG
    AAGGGTGTTT CTCAAGTCAA AGAAGGCCCT GTCCTAGCAA CTAGTTCAGG AGCTGGTGTT
    TTTAAGATGG GACGATTTCA GGTTTCTGTT GCAGCAGACG GTGCCCAGAA AGAGGGTAAA
    AATAAGTCAG AAGATGCAAA GTCTGTTCAT TTTGAATCCA GCACCTCAGA GTCCTCAGTG
    CTATCAAGTA GTAGTCCAGA GAGTACCTTG GTGAAACCAG AGCCGAATGG CATAACCATC
    CCTGGTATCT CTTCAGATGT GCCAGAGAGT GCCCACAAAA CTACTGCCTC AGAGGCAAAG
    TCAGACACTG GGCAGCCTAC CAAGGTTGGA CGTTTTCAGG TGACAACTAC AGCAAACAAA
    GTGGGTCGTT TCTCTGTATC AAAAACTGAG GACAAGATCA CTGACACAAA GAAAGAAGGA
    CCAGTGGCAT CTCCTCCTTT TATGGATTTG GAACAAGCTG TTCTTCCTGC TGTGATACCA
    AAGAAAGAGA AGCCTGAACT GTCAGAGCCT TCACATCTAA ATGGGCCGTC TTCTGACCCG
    GAGGCCGCTT TTTTAAGTAG GGATGTGGAT GATGGTTCCG GTAGTCCACA CTCGCCCCAT
    CAGCTGAGCT CAAAGAGCCT TCCTAGCCAG AATCTAAGTC AAAGCCTTAG TAATTCATTT
    AACTCCTCTT ACATGAGTAG CGACAATGAG TCAGATATCG AAGATGAAGA CTTAAAGTTA
    GAGCTGCGAC GACTACGAGA TAAACATCTC AAAGAGATTC AGGACCTGCA GAGTCGCCAG
    AAGCATGAAA TTGAATCTTT GTATACCAAA CTGGGCAAGG TGCCCCCTGC TGTTATTATT
    CCCCCAGCTG CTCCCCTTTC AGGGAGAAGA CGACGACCCA CTAAAAGCAA AGGCAGCAAA
    TCTAGTCGAA GCAGTTCCTT GGGGAATAAA AGCCCCCAGC TTTCAGGTAA CCTGTCTGGT
    CAGAGTGCAG CTTCAGTCTT GCACCCCCAG CAGACCCTCC ACCCTCCTGG CAACATCCCA
    GAGTCCGGGC AGAATCAGCT GTTACAGCCC CTTAAGCCAT CTCCCTCCAG TGACAACCTC
    TATTCAGCCT TCACCAGTGA TGGTGCCATT TCAGTACCAA GCCTTTCTGC TCCAGGTCAA
    GGAACCAGCA GCACAAACAC TGTTGGGGCA ACAGTGAACA GCCAAGCCGC CCAAGCTCAG
    CCTCCTGCCA TGACGTCCAG CAGGAAGGGC ACATTCACAG ATGACTTGCA CAAGTTGGTA
    GACAATTGGG CCCGAGATGC CATGAATCTC TCAGGCAGGA GAGGAAGCAA AGGGCACATG
    AATTACGAGG GCCCTGGAAT GGCAAGGAAG TTCTCTGCAC CTGGGCAACT GTGCATCTCC
    ATGACCTCGA ACCTGGGTGG CTCTGCCCCC ATCTCTGCAG CATCAGCTAC CTCTCTAGGT
    CACTTCACCA AGTCTATGTG CCCCCCACAG CAGTATGGCT TTCCAGCTAC CCCATTTGGC
    GCTCAATGGA GTGGGACGGG TGGCCCAGCA CCACAGCCAC TTGGCCAGTT CCAACCTGTG
    GGAACTGCCT CCTTGCAGAA TTTCAACATC AGCAATTTGC AGAAATCCAT CAGCAACCCC
    CCAGGCTCCA ACCTGCGGAC CACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011519310.1
    CDS969..7295
    Translation

    Target ORF information:

    RefSeq Version XM_011521008.2
    Organism Homo sapiens(human)
    Definition Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011521008.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGCAGGGAAT ACAGCAGACA
    GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC
    ACTACTGCAC AGCCAGTGAG TCAGCCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT
    GGAAAACAGA GTACTCAGGG AGTCTCTCAG GTTGCTCCTG CAGAGCCAGT TGCAGTAGCA
    CAGACCCAAG CTACCCAGCC GACCACTTTG GCTTCCTCTG TAGACAGTGC ACATTCAGAT
    GTTGCTTCAG GTATGAGTGA TGGCAATGAG AACGTCCCAT CTTCCAGTGG AAGGCATGAA
    GGAAGAACTA CAAAACGGCA TTACCGAAAA TCTGTAAGGA GTCGCTCTCG ACATGAAAAA
    ACTTCACGCC CAAAATTAAG AATTTTGAAT GTTTCAAATA AAGGAGACCG AGTAGTAGAA
    TGTCAATTAG AGACTCATAA TAGGAAAATG GTTACATTCA AATTTGACCT AGATGGTGAC
    AACCCCGAGG AGATAGCAAC AATTATGGTG AACAATGACT TTATTCTAGC AATAGAGAGA
    GAGTCGTTTG TGGATCAAGT GCGAGAAATT ATTGAAAAAG CTGATGAAAT GCTCAGTGAG
    GATGTCAGTG TGGAACCAGA GGGTGATCAG GGATTGGAGA GTCTACAAGG AAAGGATGAC
    TATGGCTTTT CAGGTTCTCA GAAATTGGAA GGAGAGTTCA AACAACCAAT TCCTGCGTCT
    TCCATGCCAC AGCAAATAGG CATTCCTACC AGTTCTTTAA CTCAAGTTGT TCATTCTGCG
    GGAAGGCGGT TTATAGTGAG TCCTGTGCCA GAAAGCCGAT TACGAGAATC AAAAGTTTTC
    CCCAGTGAAA TAACAGATAC AGTTGCTGCC TCTACAGCTC AGAGCCCTGG AATGAACTTG
    TCTCACTCTG CATCATCCCT TAGTCTACAA CAGGCCTTTT CTGAACTTAG ACGTGCCCAA
    ATGACAGAAG GACCCAACAC AGCACCTCCA AACTTTAGTC ATACAGGACC AACATTTCCA
    GTAGTACCTC CTTTCTTAAG TAGCATTGCT GGAGTCCCAA CCACAGCAGC AGCCACAGCA
    CCAGTCCCTG CAACAAGCAG CCCTCCTAAT GACATTTCCA CATCAGTAAT TCAGTCTGAG
    GTTACAGTGC CCACTGAAGA GGGGATTGCT GGAGTTGCCA CCAGCACAGG TGTGGTAACT
    TCAGGTGGTC TCCCCATACC ACCTGTGTCT GAATCACCAG TACTTTCCAG CGTAGTTTCA
    AGTATCACAA TACCTGCAGT TGTCTCAATA TCTACTACAT CCCCGTCACT TCAAGTCCCC
    ACATCCACAT CTGAGATCGT TGTTTCTAGT ACAGCACTGT ATCCTTCAGT AACAGTTTCA
    GCAACTTCAG CCTCTGCAGG GGGCAGTACT GCTACCCCAG GTCCTAAGCC TCCAGCTGTA
    GTATCTCAGC AGGCAGCAGG CAGCACTACT GTGGGAGCCA CATTAACATC AGTTTCTACC
    ACCACTTCAT TCCCAAGCAC AGCTTCACAG CTGTGCATTC AGCTTAGCAG CAGTACTTCT
    ACTCCTACTT TAGCTGAAAC CGTGGTAGTT AGCGCACACT CACTAGATAA GACATCTCAT
    AGCAGTACAA CTGGATTGGC TTTCTCCCTC TCTGCACCAT CTTCCTCTTC CTCTCCTGGA
    GCAGGAGTGT CTAGTTATAT TTCTCAGCCT GGTGGGCTGC ATCCTTTGGT CATTCCATCA
    GTGATAGCTT CTACTCCTAT TCTTCCCCAA GCAGCAGGAC CTACTTCTAC ACCTTTATTA
    CCCCAAGTAC CTAGTATCCC ACCCTTGGTA CAGCCTGTTG CCAATGTGCC TGCTGTACAG
    CAGACACTAA TTCATAGTCA GCCTCAACCA GCTTTGCTTC CCAACCAGCC CCATACTCAT
    TGTCCTGAAG TAGATTCTGA TACACAACCC AAAGCTCCTG GAATTGATGA CATAAAGACT
    CTAGAAGAAA AGCTGCGGTC TCTGTTCAGT GAACACAGCT CATCTGGAGC TCAGCATGCC
    TCTGTCTCAC TGGAGACCTC ACTAGTCATA GAGAGCACTG TCACACCAGG CATCCCAACT
    ACTGCTGTTG CACCAAGCAA ACTCCTGACT TCTACCACAA GTACTTGCTT ACCACCAACC
    AATTTACCAC TAGGAACAGT TGCTTTGCCA GTTACACCAG TGGTCACACC TGGGCAAGTT
    TCTACCCCAG TCAGCACTAC TACATCAGGA GTGAAACCTG GAACTGCTCC CTCCAAGCCA
    CCTCTAACTA AGGCTCCGGT GCTGCCAGTG GGTACTGAAC TTCCAGCAGG TACTCTACCC
    AGCGAGCAGC TGCCACCTTT TCCAGGACCT TCTCTAACCC AGTCCCAGCA ACCTCTAGAG
    GATCTTGATG CTCAATTGAG AAGAACACTT AGTCCAGAGA TGATCACAGT GACTTCTGCG
    GTTGGTCCTG TGTCCATGGC GGCTCCAACA GCAATCACAG AAGCAGGAAC ACAGCCTCAG
    AAGGGTGTTT CTCAAGTCAA AGAAGGCCCT GTCCTAGCAA CTAGTTCAGG AGCTGGTGTT
    TTTAAGATGG GACGATTTCA GGTTTCTGTT GCAGCAGACG GTGCCCAGAA AGAGGGTAAA
    AATAAGTCAG AAGATGCAAA GTCTGTTCAT TTTGAATCCA GCACCTCAGA GTCCTCAGTG
    CTATCAAGTA GTAGTCCAGA GAGTACCTTG GTGAAACCAG AGCCGAATGG CATAACCATC
    CCTGGTATCT CTTCAGATGT GCCAGAGAGT GCCCACAAAA CTACTGCCTC AGAGGCAAAG
    TCAGACACTG GGCAGCCTAC CAAGGTTGGA CGTTTTCAGG TGACAACTAC AGCAAACAAA
    GTGGGTCGTT TCTCTGTATC AAAAACTGAG GACAAGATCA CTGACACAAA GAAAGAAGGA
    CCAGTGGCAT CTCCTCCTTT TATGGATTTG GAACAAGCTG TTCTTCCTGC TGTGATACCA
    AAGAAAGAGA AGCCTGAACT GTCAGAGCCT TCACATCTAA ATGGGCCGTC TTCTGACCCG
    GAGGCCGCTT TTTTAAGTAG GGATGTGGAT GATGGTTCCG GTAGTCCACA CTCGCCCCAT
    CAGCTGAGCT CAAAGAGCCT TCCTAGCCAG AATCTAAGTC AAAGCCTTAG TAATTCATTT
    AACTCCTCTT ACATGAGTAG CGACAATGAG TCAGATATCG AAGATGAAGA CTTAAAGTTA
    GAGCTGCGAC GACTACGAGA TAAACATCTC AAAGAGATTC AGGACCTGCA GAGTCGCCAG
    AAGCATGAAA TTGAATCTTT GTATACCAAA CTGGGCAAGG TGCCCCCTGC TGTTATTATT
    CCCCCAGCTG CTCCCCTTTC AGGGAGAAGA CGACGACCCA CTAAAAGCAA AGGCAGCAAA
    TCTAGTCGAA GCAGTTCCTT GGGGAATAAA AGCCCCCAGC TTTCAGGTAA CCTGTCTGGT
    CAGAGTGCAG CTTCAGTCTT GCACCCCCAG CAGACCCTCC ACCCTCCTGG CAACATCCCA
    GAGTCCGGGC AGAATCAGCT GTTACAGCCC CTTAAGCCAT CTCCCTCCAG TGACAACCTC
    TATTCAGCCT TCACCAGTGA TGGTGCCATT TCAGTACCAA GCCTTTCTGC TCCAGGTCAA
    GGAACCAGCA GCACAAACAC TGTTGGGGCA ACAGTGAACA GCCAAGCCGC CCAAGCTCAG
    CCTCCTGCCA TGACGTCCAG CAGGAAGGGC ACATTCACAG ATGACTTGCA CAAGTTGGTA
    GACAATTGGG CCCGAGATGC CATGAATCTC TCAGGCAGGA GAGGAAGCAA AGGGCACATG
    AATTACGAGG GCCCTGGAAT GGCAAGGAAG TTCTCTGCAC CTGGGCAACT GTGCATCTCC
    ATGACCTCGA ACCTGGGTGG CTCTGCCCCC ATCTCTGCAG CATCAGCTAC CTCTCTAGGT
    CACTTCACCA AGTCTATGTG CCCCCCACAG CAGTATGGCT TTCCAGCTAC CCCATTTGGC
    GCTCAATGGA GTGGGACGGG TGGCCCAGCA CCACAGCCAC TTGGCCAGTT CCAACCTGTG
    GGAACTGCCT CCTTGCAGAA TTTCAACATC AGCAATTTGC AGAAATCCAT CAGCAACCCC
    CCAGGCTCCA ACCTGCGGAC CACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71625
    Clone ID Related Accession (Same CDS sequence) XM_011521009.2
    Accession Version XM_011521009.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6324bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase WNK1 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521009.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGGGAATACA GCAGACAGCC
    CCTCCTCAAC AGACAGTGCA GTATTCACTT TCACAGACAT CAACCTCCAG TGAGGCCACT
    ACTGCACAGC CAGTGAGTCA GCCTCAAGCT CCACAAGTCT TGCCTCAAGT ATCAGCTGGA
    AAACAGAGTA CTCAGGGAGT CTCTCAGGTT GCTCCTGCAG AGCCAGTTGC AGTAGCACAG
    ACCCAAGCTA CCCAGCCGAC CACTTTGGCT TCCTCTGTAG ACAGTGCACA TTCAGATGTT
    GCTTCAGGTA TGAGTGATGG CAATGAGAAC GTCCCATCTT CCAGTGGAAG GCATGAAGGA
    AGAACTACAA AACGGCATTA CCGAAAATCT GTAAGGAGTC GCTCTCGACA TGAAAAAACT
    TCACGCCCAA AATTAAGAAT TTTGAATGTT TCAAATAAAG GAGACCGAGT AGTAGAATGT
    CAATTAGAGA CTCATAATAG GAAAATGGTT ACATTCAAAT TTGACCTAGA TGGTGACAAC
    CCCGAGGAGA TAGCAACAAT TATGGTGAAC AATGACTTTA TTCTAGCAAT AGAGAGAGAG
    TCGTTTGTGG ATCAAGTGCG AGAAATTATT GAAAAAGCTG ATGAAATGCT CAGTGAGGAT
    GTCAGTGTGG AACCAGAGGG TGATCAGGGA TTGGAGAGTC TACAAGGAAA GGATGACTAT
    GGCTTTTCAG GTTCTCAGAA ATTGGAAGGA GAGTTCAAAC AACCAATTCC TGCGTCTTCC
    ATGCCACAGC AAATAGGCAT TCCTACCAGT TCTTTAACTC AAGTTGTTCA TTCTGCGGGA
    AGGCGGTTTA TAGTGAGTCC TGTGCCAGAA AGCCGATTAC GAGAATCAAA AGTTTTCCCC
    AGTGAAATAA CAGATACAGT TGCTGCCTCT ACAGCTCAGA GCCCTGGAAT GAACTTGTCT
    CACTCTGCAT CATCCCTTAG TCTACAACAG GCCTTTTCTG AACTTAGACG TGCCCAAATG
    ACAGAAGGAC CCAACACAGC ACCTCCAAAC TTTAGTCATA CAGGACCAAC ATTTCCAGTA
    GTACCTCCTT TCTTAAGTAG CATTGCTGGA GTCCCAACCA CAGCAGCAGC CACAGCACCA
    GTCCCTGCAA CAAGCAGCCC TCCTAATGAC ATTTCCACAT CAGTAATTCA GTCTGAGGTT
    ACAGTGCCCA CTGAAGAGGG GATTGCTGGA GTTGCCACCA GCACAGGTGT GGTAACTTCA
    GGTGGTCTCC CCATACCACC TGTGTCTGAA TCACCAGTAC TTTCCAGCGT AGTTTCAAGT
    ATCACAATAC CTGCAGTTGT CTCAATATCT ACTACATCCC CGTCACTTCA AGTCCCCACA
    TCCACATCTG AGATCGTTGT TTCTAGTACA GCACTGTATC CTTCAGTAAC AGTTTCAGCA
    ACTTCAGCCT CTGCAGGGGG CAGTACTGCT ACCCCAGGTC CTAAGCCTCC AGCTGTAGTA
    TCTCAGCAGG CAGCAGGCAG CACTACTGTG GGAGCCACAT TAACATCAGT TTCTACCACC
    ACTTCATTCC CAAGCACAGC TTCACAGCTG TGCATTCAGC TTAGCAGCAG TACTTCTACT
    CCTACTTTAG CTGAAACCGT GGTAGTTAGC GCACACTCAC TAGATAAGAC ATCTCATAGC
    AGTACAACTG GATTGGCTTT CTCCCTCTCT GCACCATCTT CCTCTTCCTC TCCTGGAGCA
    GGAGTGTCTA GTTATATTTC TCAGCCTGGT GGGCTGCATC CTTTGGTCAT TCCATCAGTG
    ATAGCTTCTA CTCCTATTCT TCCCCAAGCA GCAGGACCTA CTTCTACACC TTTATTACCC
    CAAGTACCTA GTATCCCACC CTTGGTACAG CCTGTTGCCA ATGTGCCTGC TGTACAGCAG
    ACACTAATTC ATAGTCAGCC TCAACCAGCT TTGCTTCCCA ACCAGCCCCA TACTCATTGT
    CCTGAAGTAG ATTCTGATAC ACAACCCAAA GCTCCTGGAA TTGATGACAT AAAGACTCTA
    GAAGAAAAGC TGCGGTCTCT GTTCAGTGAA CACAGCTCAT CTGGAGCTCA GCATGCCTCT
    GTCTCACTGG AGACCTCACT AGTCATAGAG AGCACTGTCA CACCAGGCAT CCCAACTACT
    GCTGTTGCAC CAAGCAAACT CCTGACTTCT ACCACAAGTA CTTGCTTACC ACCAACCAAT
    TTACCACTAG GAACAGTTGC TTTGCCAGTT ACACCAGTGG TCACACCTGG GCAAGTTTCT
    ACCCCAGTCA GCACTACTAC ATCAGGAGTG AAACCTGGAA CTGCTCCCTC CAAGCCACCT
    CTAACTAAGG CTCCGGTGCT GCCAGTGGGT ACTGAACTTC CAGCAGGTAC TCTACCCAGC
    GAGCAGCTGC CACCTTTTCC AGGACCTTCT CTAACCCAGT CCCAGCAACC TCTAGAGGAT
    CTTGATGCTC AATTGAGAAG AACACTTAGT CCAGAGATGA TCACAGTGAC TTCTGCGGTT
    GGTCCTGTGT CCATGGCGGC TCCAACAGCA ATCACAGAAG CAGGAACACA GCCTCAGAAG
    GGTGTTTCTC AAGTCAAAGA AGGCCCTGTC CTAGCAACTA GTTCAGGAGC TGGTGTTTTT
    AAGATGGGAC GATTTCAGGT TTCTGTTGCA GCAGACGGTG CCCAGAAAGA GGGTAAAAAT
    AAGTCAGAAG ATGCAAAGTC TGTTCATTTT GAATCCAGCA CCTCAGAGTC CTCAGTGCTA
    TCAAGTAGTA GTCCAGAGAG TACCTTGGTG AAACCAGAGC CGAATGGCAT AACCATCCCT
    GGTATCTCTT CAGATGTGCC AGAGAGTGCC CACAAAACTA CTGCCTCAGA GGCAAAGTCA
    GACACTGGGC AGCCTACCAA GGTTGGACGT TTTCAGGTGA CAACTACAGC AAACAAAGTG
    GGTCGTTTCT CTGTATCAAA AACTGAGGAC AAGATCACTG ACACAAAGAA AGAAGGACCA
    GTGGCATCTC CTCCTTTTAT GGATTTGGAA CAAGCTGTTC TTCCTGCTGT GATACCAAAG
    AAAGAGAAGC CTGAACTGTC AGAGCCTTCA CATCTAAATG GGCCGTCTTC TGACCCGGAG
    GCCGCTTTTT TAAGTAGGGA TGTGGATGAT GGTTCCGGTA GTCCACACTC GCCCCATCAG
    CTGAGCTCAA AGAGCCTTCC TAGCCAGAAT CTAAGTCAAA GCCTTAGTAA TTCATTTAAC
    TCCTCTTACA TGAGTAGCGA CAATGAGTCA GATATCGAAG ATGAAGACTT AAAGTTAGAG
    CTGCGACGAC TACGAGATAA ACATCTCAAA GAGATTCAGG ACCTGCAGAG TCGCCAGAAG
    CATGAAATTG AATCTTTGTA TACCAAACTG GGCAAGGTGC CCCCTGCTGT TATTATTCCC
    CCAGCTGCTC CCCTTTCAGG GAGAAGACGA CGACCCACTA AAAGCAAAGG CAGCAAATCT
    AGTCGAAGCA GTTCCTTGGG GAATAAAAGC CCCCAGCTTT CAGGTAACCT GTCTGGTCAG
    AGTGCAGCTT CAGTCTTGCA CCCCCAGCAG ACCCTCCACC CTCCTGGCAA CATCCCAGAG
    TCCGGGCAGA ATCAGCTGTT ACAGCCCCTT AAGCCATCTC CCTCCAGTGA CAACCTCTAT
    TCAGCCTTCA CCAGTGATGG TGCCATTTCA GTACCAAGCC TTTCTGCTCC AGGTCAAGGA
    ACCAGCAGCA CAAACACTGT TGGGGCAACA GTGAACAGCC AAGCCGCCCA AGCTCAGCCT
    CCTGCCATGA CGTCCAGCAG GAAGGGCACA TTCACAGATG ACTTGCACAA GTTGGTAGAC
    AATTGGGCCC GAGATGCCAT GAATCTCTCA GGCAGGAGAG GAAGCAAAGG GCACATGAAT
    TACGAGGGCC CTGGAATGGC AAGGAAGTTC TCTGCACCTG GGCAACTGTG CATCTCCATG
    ACCTCGAACC TGGGTGGCTC TGCCCCCATC TCTGCAGCAT CAGCTACCTC TCTAGGTCAC
    TTCACCAAGT CTATGTGCCC CCCACAGCAG TATGGCTTTC CAGCTACCCC ATTTGGCGCT
    CAATGGAGTG GGACGGGTGG CCCAGCACCA CAGCCACTTG GCCAGTTCCA ACCTGTGGGA
    ACTGCCTCCT TGCAGAATTT CAACATCAGC AATTTGCAGA AATCCATCAG CAACCCCCCA
    GGCTCCAACC TGCGGACCAC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011519311.1
    CDS969..7292
    Translation

    Target ORF information:

    RefSeq Version XM_011521009.2
    Organism Homo sapiens(human)
    Definition Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011521009.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC AGGGAATACA GCAGACAGCC
    CCTCCTCAAC AGACAGTGCA GTATTCACTT TCACAGACAT CAACCTCCAG TGAGGCCACT
    ACTGCACAGC CAGTGAGTCA GCCTCAAGCT CCACAAGTCT TGCCTCAAGT ATCAGCTGGA
    AAACAGAGTA CTCAGGGAGT CTCTCAGGTT GCTCCTGCAG AGCCAGTTGC AGTAGCACAG
    ACCCAAGCTA CCCAGCCGAC CACTTTGGCT TCCTCTGTAG ACAGTGCACA TTCAGATGTT
    GCTTCAGGTA TGAGTGATGG CAATGAGAAC GTCCCATCTT CCAGTGGAAG GCATGAAGGA
    AGAACTACAA AACGGCATTA CCGAAAATCT GTAAGGAGTC GCTCTCGACA TGAAAAAACT
    TCACGCCCAA AATTAAGAAT TTTGAATGTT TCAAATAAAG GAGACCGAGT AGTAGAATGT
    CAATTAGAGA CTCATAATAG GAAAATGGTT ACATTCAAAT TTGACCTAGA TGGTGACAAC
    CCCGAGGAGA TAGCAACAAT TATGGTGAAC AATGACTTTA TTCTAGCAAT AGAGAGAGAG
    TCGTTTGTGG ATCAAGTGCG AGAAATTATT GAAAAAGCTG ATGAAATGCT CAGTGAGGAT
    GTCAGTGTGG AACCAGAGGG TGATCAGGGA TTGGAGAGTC TACAAGGAAA GGATGACTAT
    GGCTTTTCAG GTTCTCAGAA ATTGGAAGGA GAGTTCAAAC AACCAATTCC TGCGTCTTCC
    ATGCCACAGC AAATAGGCAT TCCTACCAGT TCTTTAACTC AAGTTGTTCA TTCTGCGGGA
    AGGCGGTTTA TAGTGAGTCC TGTGCCAGAA AGCCGATTAC GAGAATCAAA AGTTTTCCCC
    AGTGAAATAA CAGATACAGT TGCTGCCTCT ACAGCTCAGA GCCCTGGAAT GAACTTGTCT
    CACTCTGCAT CATCCCTTAG TCTACAACAG GCCTTTTCTG AACTTAGACG TGCCCAAATG
    ACAGAAGGAC CCAACACAGC ACCTCCAAAC TTTAGTCATA CAGGACCAAC ATTTCCAGTA
    GTACCTCCTT TCTTAAGTAG CATTGCTGGA GTCCCAACCA CAGCAGCAGC CACAGCACCA
    GTCCCTGCAA CAAGCAGCCC TCCTAATGAC ATTTCCACAT CAGTAATTCA GTCTGAGGTT
    ACAGTGCCCA CTGAAGAGGG GATTGCTGGA GTTGCCACCA GCACAGGTGT GGTAACTTCA
    GGTGGTCTCC CCATACCACC TGTGTCTGAA TCACCAGTAC TTTCCAGCGT AGTTTCAAGT
    ATCACAATAC CTGCAGTTGT CTCAATATCT ACTACATCCC CGTCACTTCA AGTCCCCACA
    TCCACATCTG AGATCGTTGT TTCTAGTACA GCACTGTATC CTTCAGTAAC AGTTTCAGCA
    ACTTCAGCCT CTGCAGGGGG CAGTACTGCT ACCCCAGGTC CTAAGCCTCC AGCTGTAGTA
    TCTCAGCAGG CAGCAGGCAG CACTACTGTG GGAGCCACAT TAACATCAGT TTCTACCACC
    ACTTCATTCC CAAGCACAGC TTCACAGCTG TGCATTCAGC TTAGCAGCAG TACTTCTACT
    CCTACTTTAG CTGAAACCGT GGTAGTTAGC GCACACTCAC TAGATAAGAC ATCTCATAGC
    AGTACAACTG GATTGGCTTT CTCCCTCTCT GCACCATCTT CCTCTTCCTC TCCTGGAGCA
    GGAGTGTCTA GTTATATTTC TCAGCCTGGT GGGCTGCATC CTTTGGTCAT TCCATCAGTG
    ATAGCTTCTA CTCCTATTCT TCCCCAAGCA GCAGGACCTA CTTCTACACC TTTATTACCC
    CAAGTACCTA GTATCCCACC CTTGGTACAG CCTGTTGCCA ATGTGCCTGC TGTACAGCAG
    ACACTAATTC ATAGTCAGCC TCAACCAGCT TTGCTTCCCA ACCAGCCCCA TACTCATTGT
    CCTGAAGTAG ATTCTGATAC ACAACCCAAA GCTCCTGGAA TTGATGACAT AAAGACTCTA
    GAAGAAAAGC TGCGGTCTCT GTTCAGTGAA CACAGCTCAT CTGGAGCTCA GCATGCCTCT
    GTCTCACTGG AGACCTCACT AGTCATAGAG AGCACTGTCA CACCAGGCAT CCCAACTACT
    GCTGTTGCAC CAAGCAAACT CCTGACTTCT ACCACAAGTA CTTGCTTACC ACCAACCAAT
    TTACCACTAG GAACAGTTGC TTTGCCAGTT ACACCAGTGG TCACACCTGG GCAAGTTTCT
    ACCCCAGTCA GCACTACTAC ATCAGGAGTG AAACCTGGAA CTGCTCCCTC CAAGCCACCT
    CTAACTAAGG CTCCGGTGCT GCCAGTGGGT ACTGAACTTC CAGCAGGTAC TCTACCCAGC
    GAGCAGCTGC CACCTTTTCC AGGACCTTCT CTAACCCAGT CCCAGCAACC TCTAGAGGAT
    CTTGATGCTC AATTGAGAAG AACACTTAGT CCAGAGATGA TCACAGTGAC TTCTGCGGTT
    GGTCCTGTGT CCATGGCGGC TCCAACAGCA ATCACAGAAG CAGGAACACA GCCTCAGAAG
    GGTGTTTCTC AAGTCAAAGA AGGCCCTGTC CTAGCAACTA GTTCAGGAGC TGGTGTTTTT
    AAGATGGGAC GATTTCAGGT TTCTGTTGCA GCAGACGGTG CCCAGAAAGA GGGTAAAAAT
    AAGTCAGAAG ATGCAAAGTC TGTTCATTTT GAATCCAGCA CCTCAGAGTC CTCAGTGCTA
    TCAAGTAGTA GTCCAGAGAG TACCTTGGTG AAACCAGAGC CGAATGGCAT AACCATCCCT
    GGTATCTCTT CAGATGTGCC AGAGAGTGCC CACAAAACTA CTGCCTCAGA GGCAAAGTCA
    GACACTGGGC AGCCTACCAA GGTTGGACGT TTTCAGGTGA CAACTACAGC AAACAAAGTG
    GGTCGTTTCT CTGTATCAAA AACTGAGGAC AAGATCACTG ACACAAAGAA AGAAGGACCA
    GTGGCATCTC CTCCTTTTAT GGATTTGGAA CAAGCTGTTC TTCCTGCTGT GATACCAAAG
    AAAGAGAAGC CTGAACTGTC AGAGCCTTCA CATCTAAATG GGCCGTCTTC TGACCCGGAG
    GCCGCTTTTT TAAGTAGGGA TGTGGATGAT GGTTCCGGTA GTCCACACTC GCCCCATCAG
    CTGAGCTCAA AGAGCCTTCC TAGCCAGAAT CTAAGTCAAA GCCTTAGTAA TTCATTTAAC
    TCCTCTTACA TGAGTAGCGA CAATGAGTCA GATATCGAAG ATGAAGACTT AAAGTTAGAG
    CTGCGACGAC TACGAGATAA ACATCTCAAA GAGATTCAGG ACCTGCAGAG TCGCCAGAAG
    CATGAAATTG AATCTTTGTA TACCAAACTG GGCAAGGTGC CCCCTGCTGT TATTATTCCC
    CCAGCTGCTC CCCTTTCAGG GAGAAGACGA CGACCCACTA AAAGCAAAGG CAGCAAATCT
    AGTCGAAGCA GTTCCTTGGG GAATAAAAGC CCCCAGCTTT CAGGTAACCT GTCTGGTCAG
    AGTGCAGCTT CAGTCTTGCA CCCCCAGCAG ACCCTCCACC CTCCTGGCAA CATCCCAGAG
    TCCGGGCAGA ATCAGCTGTT ACAGCCCCTT AAGCCATCTC CCTCCAGTGA CAACCTCTAT
    TCAGCCTTCA CCAGTGATGG TGCCATTTCA GTACCAAGCC TTTCTGCTCC AGGTCAAGGA
    ACCAGCAGCA CAAACACTGT TGGGGCAACA GTGAACAGCC AAGCCGCCCA AGCTCAGCCT
    CCTGCCATGA CGTCCAGCAG GAAGGGCACA TTCACAGATG ACTTGCACAA GTTGGTAGAC
    AATTGGGCCC GAGATGCCAT GAATCTCTCA GGCAGGAGAG GAAGCAAAGG GCACATGAAT
    TACGAGGGCC CTGGAATGGC AAGGAAGTTC TCTGCACCTG GGCAACTGTG CATCTCCATG
    ACCTCGAACC TGGGTGGCTC TGCCCCCATC TCTGCAGCAT CAGCTACCTC TCTAGGTCAC
    TTCACCAAGT CTATGTGCCC CCCACAGCAG TATGGCTTTC CAGCTACCCC ATTTGGCGCT
    CAATGGAGTG GGACGGGTGG CCCAGCACCA CAGCCACTTG GCCAGTTCCA ACCTGTGGGA
    ACTGCCTCCT TGCAGAATTT CAACATCAGC AATTTGCAGA AATCCATCAG CAACCCCCCA
    GGCTCCAACC TGCGGACCAC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71613
    Clone ID Related Accession (Same CDS sequence) XM_011520997.2 , XM_011520997.3
    Accession Version XM_011520997.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8388bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase WNK1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011520997.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG
    TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT
    CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT
    AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT
    GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT
    ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT
    ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA
    GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG
    GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA
    CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT
    CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG
    GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG
    AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG
    AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA
    GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT
    CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA
    GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG
    AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC
    CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC
    GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC
    TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA
    TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA
    ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGCAGGG AATACAGCAG
    ACAGCCCCTC CTCAACAGAC AGTGCAGTAT TCACTTTCAC AGACATCAAC CTCCAGTGAG
    GCCACTACTG CACAGCCAGT GAGTCAGCCT CAAGCTCCAC AAGTCTTGCC TCAAGTATCA
    GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA GTACCAACTA TCCAAGGCGA ACCTCAGATC
    CCAGTTGCGA CACAACCCTC GGTTGTTCCA GTCCACTCTG GTGCTCATTT CCTTCCAGTG
    GGACAGCCGC TCCCTACTCC CTTGCTCCCT CAGTACCCTG TCTCTCAGAT TCCCATATCA
    ACTCCTCATG TGTCTACGGC TCAGACAGGT TTCTCATCCC TTCCCATCAC AATGGCAGCT
    GGCATTACTC AGCCTCTGCT CACGTTGGCT TCATCTGCTA CAACAGCTGC GATCCCGGGG
    GTATCAACTG TGGTTCCTAG TCAGCTTCCA ACCCTTCTGC AGCCTGTGAC TCAGCTGCCA
    AGTCAGGTTC ACCCACAGCT CCTACAACCA GCAGTTCAGT CCATGGGAAT ACCAGCTAAC
    CTTGGACAAG CTGCTGAGGT TCCACTTTCC TCTGGAGATG TTCTGTACCA GGGCTTCCCA
    CCTCGACTGC CACCACAGTA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT
    TCTGTTTGCA TCCATTCTAC AGTCCTATCC CCTCCCATGC CGACAGAAGT ACTGGCTACA
    CCTGGGTACT TTCCCACAGT GGTGCAGCCT TATGTGGAAT CAAATCTTTT AGTTCCTATG
    GGTGGTGTAG GAGGACAGGT TCAAGTGTCC CAGCCAGGAG GGAGTTTAGC ACAAGCCCCC
    ACTACATCCT CCCAGCAAGC AGTTTTGGAG AGTACTCAGG GAGTCTCTCA GGTTGCTCCT
    GCAGAGCCAG TTGCAGTAGC ACAGACCCAA GCTACCCAGC CGACCACTTT GGCTTCCTCT
    GTAGACAGTG CACATTCAGA TGTTGCTTCA GGTATGAGTG ATGGCAATGA GAACGTCCCA
    TCTTCCAGTG GAAGGCATGA AGGAAGAACT ACAAAACGGC ATTACCGAAA ATCTGTAAGG
    AGTCGCTCTC GACATGAAAA AACTTCACGC CCAAAATTAA GAATTTTGAA TGTTTCAAAT
    AAAGGAGACC GAGTAGTAGA ATGTCAATTA GAGACTCATA ATAGGAAAAT GGTTACATTC
    AAATTTGACC TAGATGGTGA CAACCCCGAG GAGATAGCAA CAATTATGGT GAACAATGAC
    TTTATTCTAG CAATAGAGAG AGAGTCGTTT GTGGATCAAG TGCGAGAAAT TATTGAAAAA
    GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGATCA GGGATTGGAG
    AGTCTACAAG GAAAGGATGA CTATGGCTTT TCAGGTTCTC AGAAATTGGA AGGAGAGTTC
    AAACAACCAA TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA
    ACTCAAGTTG TTCATTCTGC GGGAAGGCGG TTTATAGTGA GTCCTGTGCC AGAAAGCCGA
    TTACGAGAAT CAAAAGTTTT CCCCAGTGAA ATAACAGATA CAGTTGCTGC CTCTACAGCT
    CAGAGCCCTG GAATGAACTT GTCTCACTCT GCATCATCCC TTAGTCTACA ACAGGCCTTT
    TCTGAACTTA GACGTGCCCA AATGACAGAA GGACCCAACA CAGCACCTCC AAACTTTAGT
    CATACAGGAC CAACATTTCC AGTAGTACCT CCTTTCTTAA GTAGCATTGC TGGAGTCCCA
    ACCACAGCAG CAGCCACAGC ACCAGTCCCT GCAACAAGCA GCCCTCCTAA TGACATTTCC
    ACATCAGTAA TTCAGTCTGA GGTTACAGTG CCCACTGAAG AGGGGATTGC TGGAGTTGCC
    ACCAGCACAG GTGTGGTAAC TTCAGGTGGT CTCCCCATAC CACCTGTGTC TGAATCACCA
    GTACTTTCCA GCGTAGTTTC AAGTATCACA ATACCTGCAG TTGTCTCAAT ATCTACTACA
    TCCCCGTCAC TTCAAGTCCC CACATCCACA TCTGAGATCG TTGTTTCTAG TACAGCACTG
    TATCCTTCAG TAACAGTTTC AGCAACTTCA GCCTCTGCAG GGGGCAGTAC TGCTACCCCA
    GGTCCTAAGC CTCCAGCTGT AGTATCTCAG CAGGCAGCAG GCAGCACTAC TGTGGGAGCC
    ACATTAACAT CAGTTTCTAC CACCACTTCA TTCCCAAGCA CAGCTTCACA GCTGTGCATT
    CAGCTTAGCA GCAGTACTTC TACTCCTACT TTAGCTGAAA CCGTGGTAGT TAGCGCACAC
    TCACTAGATA AGACATCTCA TAGCAGTACA ACTGGATTGG CTTTCTCCCT CTCTGCACCA
    TCTTCCTCTT CCTCTCCTGG AGCAGGAGTG TCTAGTTATA TTTCTCAGCC TGGTGGGCTG
    CATCCTTTGG TCATTCCATC AGTGATAGCT TCTACTCCTA TTCTTCCCCA AGCAGCAGGA
    CCTACTTCTA CACCTTTATT ACCCCAAGTA CCTAGTATCC CACCCTTGGT ACAGCCTGTT
    GCCAATGTGC CTGCTGTACA GCAGACACTA ATTCATAGTC AGCCTCAACC AGCTTTGCTT
    CCCAACCAGC CCCATACTCA TTGTCCTGAA GTAGATTCTG ATACACAACC CAAAGCTCCT
    GGAATTGATG ACATAAAGAC TCTAGAAGAA AAGCTGCGGT CTCTGTTCAG TGAACACAGC
    TCATCTGGAG CTCAGCATGC CTCTGTCTCA CTGGAGACCT CACTAGTCAT AGAGAGCACT
    GTCACACCAG GCATCCCAAC TACTGCTGTT GCACCAAGCA AACTCCTGAC TTCTACCACA
    AGTACTTGCT TACCACCAAC CAATTTACCA CTAGGAACAG TTGCTTTGCC AGTTACACCA
    GTGGTCACAC CTGGGCAAGT TTCTACCCCA GTCAGCACTA CTACATCAGG AGTGAAACCT
    GGAACTGCTC CCTCCAAGCC ACCTCTAACT AAGGCTCCGG TGCTGCCAGT GGGTACTGAA
    CTTCCAGCAG GTACTCTACC CAGCGAGCAG CTGCCACCTT TTCCAGGACC TTCTCTAACC
    CAGTCCCAGC AACCTCTAGA GGATCTTGAT GCTCAATTGA GAAGAACACT TAGTCCAGAG
    ATGATCACAG TGACTTCTGC GGTTGGTCCT GTGTCCATGG CGGCTCCAAC AGCAATCACA
    GAAGCAGGAA CACAGCCTCA GAAGGGTGTT TCTCAAGTCA AAGAAGGCCC TGTCCTAGCA
    ACTAGTTCAG GAGCTGGTGT TTTTAAGATG GGACGATTTC AGGTTTCTGT TGCAGCAGAC
    GGTGCCCAGA AAGAGGGTAA AAATAAGTCA GAAGATGCAA AGTCTGTTCA TTTTGAATCC
    AGCACCTCAG AGTCCTCAGT GCTATCAAGT AGTAGTCCAG AGAGTACCTT GGTGAAACCA
    GAGCCGAATG GCATAACCAT CCCTGGTATC TCTTCAGATG TGCCAGAGAG TGCCCACAAA
    ACTACTGCCT CAGAGGCAAA GTCAGACACT GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG
    GTGACAACTA CAGCAAACAA AGTGGGTCGT TTCTCTGTAT CAAAAACTGA GGACAAGATC
    ACTGACACAA AGAAAGAAGG ACCAGTGGCA TCTCCTCCTT TTATGGATTT GGAACAAGCT
    GTTCTTCCTG CTGTGATACC AAAGAAAGAG AAGCCTGAAC TGTCAGAGCC TTCACATCTA
    AATGGGCCGT CTTCTGACCC GGAGGCCGCT TTTTTAAGTA GGGATGTGGA TGATGGTTCC
    GGTAGTCCAC ACTCGCCCCA TCAGCTGAGC TCAAAGAGCC TTCCTAGCCA GAATCTAAGT
    CAAAGCCTTA GTAATTCATT TAACTCCTCT TACATGAGTA GCGACAATGA GTCAGATATC
    GAAGATGAAG ACTTAAAGTT AGAGCTGCGA CGACTACGAG ATAAACATCT CAAAGAGATT
    CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACCAA ACTGGGCAAG
    GTGCCCCCTG CTGTTATTAT TCCCCCAGCT GCTCCCCTTT CAGGGAGAAG ACGACGACCC
    ACTAAAAGCA AAGGCAGCAA ATCTAGTCGA AGCAGTTCCT TGGGGAATAA AAGCCCCCAG
    CTTTCAGGTA ACCTGTCTGG TCAGAGTGCA GCTTCAGTCT TGCACCCCCA GCAGACCCTC
    CACCCTCCTG GCAACATCCC AGAGTCCGGG CAGAATCAGC TGTTACAGCC CCTTAAGCCA
    TCTCCCTCCA GTGACAACCT CTATTCAGCC TTCACCAGTG ATGGTGCCAT TTCAGTACCA
    AGCCTTTCTG CTCCAGGTCA AGGAACCAGC AGCACAAACA CTGTTGGGGC AACAGTGAAC
    AGCCAAGCCG CCCAAGCTCA GCCTCCTGCC ATGACGTCCA GCAGGAAGGG CACATTCACA
    GATGACTTGC ACAAGTTGGT AGACAATTGG GCCCGAGATG CCATGAATCT CTCAGGCAGG
    AGAGGAAGCA AAGGGCACAT GAATTACGAG GGCCCTGGAA TGGCAAGGAA GTTCTCTGCA
    CCTGGGCAAC TGTGCATCTC CATGACCTCG AACCTGGGTG GCTCTGCCCC CATCTCTGCA
    GCATCAGCTA CCTCTCTAGG TCACTTCACC AAGTCTATGT GCCCCCCACA GCAGTATGGC
    TTTCCAGCTA CCCCATTTGG CGCTCAATGG AGTGGGACGG GTGGCCCAGC ACCACAGCCA
    CTTGGCCAGT TCCAACCTGT GGGAACTGCC TCCTTGCAGA ATTTCAACAT CAGCAATTTG
    CAGAAATCCA TCAGCAACCC CCCAGGCTCC AACCTGCGGA CCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011519299.1
    CDS1015..9402
    Translation

    Target ORF information:

    RefSeq Version XM_011520997.2
    Organism Homo sapiens(human)
    Definition Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011520997.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG
    TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT
    CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT
    AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT
    GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT
    ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT
    ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA
    GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG
    GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA
    CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT
    CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG
    GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG
    AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG
    AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA
    GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT
    CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA
    GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG
    AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC
    CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC
    GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC
    TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA
    TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA
    ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGCAGGG AATACAGCAG
    ACAGCCCCTC CTCAACAGAC AGTGCAGTAT TCACTTTCAC AGACATCAAC CTCCAGTGAG
    GCCACTACTG CACAGCCAGT GAGTCAGCCT CAAGCTCCAC AAGTCTTGCC TCAAGTATCA
    GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA GTACCAACTA TCCAAGGCGA ACCTCAGATC
    CCAGTTGCGA CACAACCCTC GGTTGTTCCA GTCCACTCTG GTGCTCATTT CCTTCCAGTG
    GGACAGCCGC TCCCTACTCC CTTGCTCCCT CAGTACCCTG TCTCTCAGAT TCCCATATCA
    ACTCCTCATG TGTCTACGGC TCAGACAGGT TTCTCATCCC TTCCCATCAC AATGGCAGCT
    GGCATTACTC AGCCTCTGCT CACGTTGGCT TCATCTGCTA CAACAGCTGC GATCCCGGGG
    GTATCAACTG TGGTTCCTAG TCAGCTTCCA ACCCTTCTGC AGCCTGTGAC TCAGCTGCCA
    AGTCAGGTTC ACCCACAGCT CCTACAACCA GCAGTTCAGT CCATGGGAAT ACCAGCTAAC
    CTTGGACAAG CTGCTGAGGT TCCACTTTCC TCTGGAGATG TTCTGTACCA GGGCTTCCCA
    CCTCGACTGC CACCACAGTA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT
    TCTGTTTGCA TCCATTCTAC AGTCCTATCC CCTCCCATGC CGACAGAAGT ACTGGCTACA
    CCTGGGTACT TTCCCACAGT GGTGCAGCCT TATGTGGAAT CAAATCTTTT AGTTCCTATG
    GGTGGTGTAG GAGGACAGGT TCAAGTGTCC CAGCCAGGAG GGAGTTTAGC ACAAGCCCCC
    ACTACATCCT CCCAGCAAGC AGTTTTGGAG AGTACTCAGG GAGTCTCTCA GGTTGCTCCT
    GCAGAGCCAG TTGCAGTAGC ACAGACCCAA GCTACCCAGC CGACCACTTT GGCTTCCTCT
    GTAGACAGTG CACATTCAGA TGTTGCTTCA GGTATGAGTG ATGGCAATGA GAACGTCCCA
    TCTTCCAGTG GAAGGCATGA AGGAAGAACT ACAAAACGGC ATTACCGAAA ATCTGTAAGG
    AGTCGCTCTC GACATGAAAA AACTTCACGC CCAAAATTAA GAATTTTGAA TGTTTCAAAT
    AAAGGAGACC GAGTAGTAGA ATGTCAATTA GAGACTCATA ATAGGAAAAT GGTTACATTC
    AAATTTGACC TAGATGGTGA CAACCCCGAG GAGATAGCAA CAATTATGGT GAACAATGAC
    TTTATTCTAG CAATAGAGAG AGAGTCGTTT GTGGATCAAG TGCGAGAAAT TATTGAAAAA
    GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGATCA GGGATTGGAG
    AGTCTACAAG GAAAGGATGA CTATGGCTTT TCAGGTTCTC AGAAATTGGA AGGAGAGTTC
    AAACAACCAA TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA
    ACTCAAGTTG TTCATTCTGC GGGAAGGCGG TTTATAGTGA GTCCTGTGCC AGAAAGCCGA
    TTACGAGAAT CAAAAGTTTT CCCCAGTGAA ATAACAGATA CAGTTGCTGC CTCTACAGCT
    CAGAGCCCTG GAATGAACTT GTCTCACTCT GCATCATCCC TTAGTCTACA ACAGGCCTTT
    TCTGAACTTA GACGTGCCCA AATGACAGAA GGACCCAACA CAGCACCTCC AAACTTTAGT
    CATACAGGAC CAACATTTCC AGTAGTACCT CCTTTCTTAA GTAGCATTGC TGGAGTCCCA
    ACCACAGCAG CAGCCACAGC ACCAGTCCCT GCAACAAGCA GCCCTCCTAA TGACATTTCC
    ACATCAGTAA TTCAGTCTGA GGTTACAGTG CCCACTGAAG AGGGGATTGC TGGAGTTGCC
    ACCAGCACAG GTGTGGTAAC TTCAGGTGGT CTCCCCATAC CACCTGTGTC TGAATCACCA
    GTACTTTCCA GCGTAGTTTC AAGTATCACA ATACCTGCAG TTGTCTCAAT ATCTACTACA
    TCCCCGTCAC TTCAAGTCCC CACATCCACA TCTGAGATCG TTGTTTCTAG TACAGCACTG
    TATCCTTCAG TAACAGTTTC AGCAACTTCA GCCTCTGCAG GGGGCAGTAC TGCTACCCCA
    GGTCCTAAGC CTCCAGCTGT AGTATCTCAG CAGGCAGCAG GCAGCACTAC TGTGGGAGCC
    ACATTAACAT CAGTTTCTAC CACCACTTCA TTCCCAAGCA CAGCTTCACA GCTGTGCATT
    CAGCTTAGCA GCAGTACTTC TACTCCTACT TTAGCTGAAA CCGTGGTAGT TAGCGCACAC
    TCACTAGATA AGACATCTCA TAGCAGTACA ACTGGATTGG CTTTCTCCCT CTCTGCACCA
    TCTTCCTCTT CCTCTCCTGG AGCAGGAGTG TCTAGTTATA TTTCTCAGCC TGGTGGGCTG
    CATCCTTTGG TCATTCCATC AGTGATAGCT TCTACTCCTA TTCTTCCCCA AGCAGCAGGA
    CCTACTTCTA CACCTTTATT ACCCCAAGTA CCTAGTATCC CACCCTTGGT ACAGCCTGTT
    GCCAATGTGC CTGCTGTACA GCAGACACTA ATTCATAGTC AGCCTCAACC AGCTTTGCTT
    CCCAACCAGC CCCATACTCA TTGTCCTGAA GTAGATTCTG ATACACAACC CAAAGCTCCT
    GGAATTGATG ACATAAAGAC TCTAGAAGAA AAGCTGCGGT CTCTGTTCAG TGAACACAGC
    TCATCTGGAG CTCAGCATGC CTCTGTCTCA CTGGAGACCT CACTAGTCAT AGAGAGCACT
    GTCACACCAG GCATCCCAAC TACTGCTGTT GCACCAAGCA AACTCCTGAC TTCTACCACA
    AGTACTTGCT TACCACCAAC CAATTTACCA CTAGGAACAG TTGCTTTGCC AGTTACACCA
    GTGGTCACAC CTGGGCAAGT TTCTACCCCA GTCAGCACTA CTACATCAGG AGTGAAACCT
    GGAACTGCTC CCTCCAAGCC ACCTCTAACT AAGGCTCCGG TGCTGCCAGT GGGTACTGAA
    CTTCCAGCAG GTACTCTACC CAGCGAGCAG CTGCCACCTT TTCCAGGACC TTCTCTAACC
    CAGTCCCAGC AACCTCTAGA GGATCTTGAT GCTCAATTGA GAAGAACACT TAGTCCAGAG
    ATGATCACAG TGACTTCTGC GGTTGGTCCT GTGTCCATGG CGGCTCCAAC AGCAATCACA
    GAAGCAGGAA CACAGCCTCA GAAGGGTGTT TCTCAAGTCA AAGAAGGCCC TGTCCTAGCA
    ACTAGTTCAG GAGCTGGTGT TTTTAAGATG GGACGATTTC AGGTTTCTGT TGCAGCAGAC
    GGTGCCCAGA AAGAGGGTAA AAATAAGTCA GAAGATGCAA AGTCTGTTCA TTTTGAATCC
    AGCACCTCAG AGTCCTCAGT GCTATCAAGT AGTAGTCCAG AGAGTACCTT GGTGAAACCA
    GAGCCGAATG GCATAACCAT CCCTGGTATC TCTTCAGATG TGCCAGAGAG TGCCCACAAA
    ACTACTGCCT CAGAGGCAAA GTCAGACACT GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG
    GTGACAACTA CAGCAAACAA AGTGGGTCGT TTCTCTGTAT CAAAAACTGA GGACAAGATC
    ACTGACACAA AGAAAGAAGG ACCAGTGGCA TCTCCTCCTT TTATGGATTT GGAACAAGCT
    GTTCTTCCTG CTGTGATACC AAAGAAAGAG AAGCCTGAAC TGTCAGAGCC TTCACATCTA
    AATGGGCCGT CTTCTGACCC GGAGGCCGCT TTTTTAAGTA GGGATGTGGA TGATGGTTCC
    GGTAGTCCAC ACTCGCCCCA TCAGCTGAGC TCAAAGAGCC TTCCTAGCCA GAATCTAAGT
    CAAAGCCTTA GTAATTCATT TAACTCCTCT TACATGAGTA GCGACAATGA GTCAGATATC
    GAAGATGAAG ACTTAAAGTT AGAGCTGCGA CGACTACGAG ATAAACATCT CAAAGAGATT
    CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACCAA ACTGGGCAAG
    GTGCCCCCTG CTGTTATTAT TCCCCCAGCT GCTCCCCTTT CAGGGAGAAG ACGACGACCC
    ACTAAAAGCA AAGGCAGCAA ATCTAGTCGA AGCAGTTCCT TGGGGAATAA AAGCCCCCAG
    CTTTCAGGTA ACCTGTCTGG TCAGAGTGCA GCTTCAGTCT TGCACCCCCA GCAGACCCTC
    CACCCTCCTG GCAACATCCC AGAGTCCGGG CAGAATCAGC TGTTACAGCC CCTTAAGCCA
    TCTCCCTCCA GTGACAACCT CTATTCAGCC TTCACCAGTG ATGGTGCCAT TTCAGTACCA
    AGCCTTTCTG CTCCAGGTCA AGGAACCAGC AGCACAAACA CTGTTGGGGC AACAGTGAAC
    AGCCAAGCCG CCCAAGCTCA GCCTCCTGCC ATGACGTCCA GCAGGAAGGG CACATTCACA
    GATGACTTGC ACAAGTTGGT AGACAATTGG GCCCGAGATG CCATGAATCT CTCAGGCAGG
    AGAGGAAGCA AAGGGCACAT GAATTACGAG GGCCCTGGAA TGGCAAGGAA GTTCTCTGCA
    CCTGGGCAAC TGTGCATCTC CATGACCTCG AACCTGGGTG GCTCTGCCCC CATCTCTGCA
    GCATCAGCTA CCTCTCTAGG TCACTTCACC AAGTCTATGT GCCCCCCACA GCAGTATGGC
    TTTCCAGCTA CCCCATTTGG CGCTCAATGG AGTGGGACGG GTGGCCCAGC ACCACAGCCA
    CTTGGCCAGT TCCAACCTGT GGGAACTGCC TCCTTGCAGA ATTTCAACAT CAGCAATTTG
    CAGAAATCCA TCAGCAACCC CCCAGGCTCC AACCTGCGGA CCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71613
    Clone ID Related Accession (Same CDS sequence) XM_011520997.2 , XM_011520997.3
    Accession Version XM_011520997.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8388bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase WNK1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011520997.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG
    TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT
    CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT
    AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT
    GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT
    ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT
    ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA
    GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG
    GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA
    CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT
    CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG
    GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG
    AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG
    AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA
    GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT
    CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA
    GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG
    AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC
    CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC
    GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC
    TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA
    TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA
    ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGCAGGG AATACAGCAG
    ACAGCCCCTC CTCAACAGAC AGTGCAGTAT TCACTTTCAC AGACATCAAC CTCCAGTGAG
    GCCACTACTG CACAGCCAGT GAGTCAGCCT CAAGCTCCAC AAGTCTTGCC TCAAGTATCA
    GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA GTACCAACTA TCCAAGGCGA ACCTCAGATC
    CCAGTTGCGA CACAACCCTC GGTTGTTCCA GTCCACTCTG GTGCTCATTT CCTTCCAGTG
    GGACAGCCGC TCCCTACTCC CTTGCTCCCT CAGTACCCTG TCTCTCAGAT TCCCATATCA
    ACTCCTCATG TGTCTACGGC TCAGACAGGT TTCTCATCCC TTCCCATCAC AATGGCAGCT
    GGCATTACTC AGCCTCTGCT CACGTTGGCT TCATCTGCTA CAACAGCTGC GATCCCGGGG
    GTATCAACTG TGGTTCCTAG TCAGCTTCCA ACCCTTCTGC AGCCTGTGAC TCAGCTGCCA
    AGTCAGGTTC ACCCACAGCT CCTACAACCA GCAGTTCAGT CCATGGGAAT ACCAGCTAAC
    CTTGGACAAG CTGCTGAGGT TCCACTTTCC TCTGGAGATG TTCTGTACCA GGGCTTCCCA
    CCTCGACTGC CACCACAGTA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT
    TCTGTTTGCA TCCATTCTAC AGTCCTATCC CCTCCCATGC CGACAGAAGT ACTGGCTACA
    CCTGGGTACT TTCCCACAGT GGTGCAGCCT TATGTGGAAT CAAATCTTTT AGTTCCTATG
    GGTGGTGTAG GAGGACAGGT TCAAGTGTCC CAGCCAGGAG GGAGTTTAGC ACAAGCCCCC
    ACTACATCCT CCCAGCAAGC AGTTTTGGAG AGTACTCAGG GAGTCTCTCA GGTTGCTCCT
    GCAGAGCCAG TTGCAGTAGC ACAGACCCAA GCTACCCAGC CGACCACTTT GGCTTCCTCT
    GTAGACAGTG CACATTCAGA TGTTGCTTCA GGTATGAGTG ATGGCAATGA GAACGTCCCA
    TCTTCCAGTG GAAGGCATGA AGGAAGAACT ACAAAACGGC ATTACCGAAA ATCTGTAAGG
    AGTCGCTCTC GACATGAAAA AACTTCACGC CCAAAATTAA GAATTTTGAA TGTTTCAAAT
    AAAGGAGACC GAGTAGTAGA ATGTCAATTA GAGACTCATA ATAGGAAAAT GGTTACATTC
    AAATTTGACC TAGATGGTGA CAACCCCGAG GAGATAGCAA CAATTATGGT GAACAATGAC
    TTTATTCTAG CAATAGAGAG AGAGTCGTTT GTGGATCAAG TGCGAGAAAT TATTGAAAAA
    GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGATCA GGGATTGGAG
    AGTCTACAAG GAAAGGATGA CTATGGCTTT TCAGGTTCTC AGAAATTGGA AGGAGAGTTC
    AAACAACCAA TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA
    ACTCAAGTTG TTCATTCTGC GGGAAGGCGG TTTATAGTGA GTCCTGTGCC AGAAAGCCGA
    TTACGAGAAT CAAAAGTTTT CCCCAGTGAA ATAACAGATA CAGTTGCTGC CTCTACAGCT
    CAGAGCCCTG GAATGAACTT GTCTCACTCT GCATCATCCC TTAGTCTACA ACAGGCCTTT
    TCTGAACTTA GACGTGCCCA AATGACAGAA GGACCCAACA CAGCACCTCC AAACTTTAGT
    CATACAGGAC CAACATTTCC AGTAGTACCT CCTTTCTTAA GTAGCATTGC TGGAGTCCCA
    ACCACAGCAG CAGCCACAGC ACCAGTCCCT GCAACAAGCA GCCCTCCTAA TGACATTTCC
    ACATCAGTAA TTCAGTCTGA GGTTACAGTG CCCACTGAAG AGGGGATTGC TGGAGTTGCC
    ACCAGCACAG GTGTGGTAAC TTCAGGTGGT CTCCCCATAC CACCTGTGTC TGAATCACCA
    GTACTTTCCA GCGTAGTTTC AAGTATCACA ATACCTGCAG TTGTCTCAAT ATCTACTACA
    TCCCCGTCAC TTCAAGTCCC CACATCCACA TCTGAGATCG TTGTTTCTAG TACAGCACTG
    TATCCTTCAG TAACAGTTTC AGCAACTTCA GCCTCTGCAG GGGGCAGTAC TGCTACCCCA
    GGTCCTAAGC CTCCAGCTGT AGTATCTCAG CAGGCAGCAG GCAGCACTAC TGTGGGAGCC
    ACATTAACAT CAGTTTCTAC CACCACTTCA TTCCCAAGCA CAGCTTCACA GCTGTGCATT
    CAGCTTAGCA GCAGTACTTC TACTCCTACT TTAGCTGAAA CCGTGGTAGT TAGCGCACAC
    TCACTAGATA AGACATCTCA TAGCAGTACA ACTGGATTGG CTTTCTCCCT CTCTGCACCA
    TCTTCCTCTT CCTCTCCTGG AGCAGGAGTG TCTAGTTATA TTTCTCAGCC TGGTGGGCTG
    CATCCTTTGG TCATTCCATC AGTGATAGCT TCTACTCCTA TTCTTCCCCA AGCAGCAGGA
    CCTACTTCTA CACCTTTATT ACCCCAAGTA CCTAGTATCC CACCCTTGGT ACAGCCTGTT
    GCCAATGTGC CTGCTGTACA GCAGACACTA ATTCATAGTC AGCCTCAACC AGCTTTGCTT
    CCCAACCAGC CCCATACTCA TTGTCCTGAA GTAGATTCTG ATACACAACC CAAAGCTCCT
    GGAATTGATG ACATAAAGAC TCTAGAAGAA AAGCTGCGGT CTCTGTTCAG TGAACACAGC
    TCATCTGGAG CTCAGCATGC CTCTGTCTCA CTGGAGACCT CACTAGTCAT AGAGAGCACT
    GTCACACCAG GCATCCCAAC TACTGCTGTT GCACCAAGCA AACTCCTGAC TTCTACCACA
    AGTACTTGCT TACCACCAAC CAATTTACCA CTAGGAACAG TTGCTTTGCC AGTTACACCA
    GTGGTCACAC CTGGGCAAGT TTCTACCCCA GTCAGCACTA CTACATCAGG AGTGAAACCT
    GGAACTGCTC CCTCCAAGCC ACCTCTAACT AAGGCTCCGG TGCTGCCAGT GGGTACTGAA
    CTTCCAGCAG GTACTCTACC CAGCGAGCAG CTGCCACCTT TTCCAGGACC TTCTCTAACC
    CAGTCCCAGC AACCTCTAGA GGATCTTGAT GCTCAATTGA GAAGAACACT TAGTCCAGAG
    ATGATCACAG TGACTTCTGC GGTTGGTCCT GTGTCCATGG CGGCTCCAAC AGCAATCACA
    GAAGCAGGAA CACAGCCTCA GAAGGGTGTT TCTCAAGTCA AAGAAGGCCC TGTCCTAGCA
    ACTAGTTCAG GAGCTGGTGT TTTTAAGATG GGACGATTTC AGGTTTCTGT TGCAGCAGAC
    GGTGCCCAGA AAGAGGGTAA AAATAAGTCA GAAGATGCAA AGTCTGTTCA TTTTGAATCC
    AGCACCTCAG AGTCCTCAGT GCTATCAAGT AGTAGTCCAG AGAGTACCTT GGTGAAACCA
    GAGCCGAATG GCATAACCAT CCCTGGTATC TCTTCAGATG TGCCAGAGAG TGCCCACAAA
    ACTACTGCCT CAGAGGCAAA GTCAGACACT GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG
    GTGACAACTA CAGCAAACAA AGTGGGTCGT TTCTCTGTAT CAAAAACTGA GGACAAGATC
    ACTGACACAA AGAAAGAAGG ACCAGTGGCA TCTCCTCCTT TTATGGATTT GGAACAAGCT
    GTTCTTCCTG CTGTGATACC AAAGAAAGAG AAGCCTGAAC TGTCAGAGCC TTCACATCTA
    AATGGGCCGT CTTCTGACCC GGAGGCCGCT TTTTTAAGTA GGGATGTGGA TGATGGTTCC
    GGTAGTCCAC ACTCGCCCCA TCAGCTGAGC TCAAAGAGCC TTCCTAGCCA GAATCTAAGT
    CAAAGCCTTA GTAATTCATT TAACTCCTCT TACATGAGTA GCGACAATGA GTCAGATATC
    GAAGATGAAG ACTTAAAGTT AGAGCTGCGA CGACTACGAG ATAAACATCT CAAAGAGATT
    CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACCAA ACTGGGCAAG
    GTGCCCCCTG CTGTTATTAT TCCCCCAGCT GCTCCCCTTT CAGGGAGAAG ACGACGACCC
    ACTAAAAGCA AAGGCAGCAA ATCTAGTCGA AGCAGTTCCT TGGGGAATAA AAGCCCCCAG
    CTTTCAGGTA ACCTGTCTGG TCAGAGTGCA GCTTCAGTCT TGCACCCCCA GCAGACCCTC
    CACCCTCCTG GCAACATCCC AGAGTCCGGG CAGAATCAGC TGTTACAGCC CCTTAAGCCA
    TCTCCCTCCA GTGACAACCT CTATTCAGCC TTCACCAGTG ATGGTGCCAT TTCAGTACCA
    AGCCTTTCTG CTCCAGGTCA AGGAACCAGC AGCACAAACA CTGTTGGGGC AACAGTGAAC
    AGCCAAGCCG CCCAAGCTCA GCCTCCTGCC ATGACGTCCA GCAGGAAGGG CACATTCACA
    GATGACTTGC ACAAGTTGGT AGACAATTGG GCCCGAGATG CCATGAATCT CTCAGGCAGG
    AGAGGAAGCA AAGGGCACAT GAATTACGAG GGCCCTGGAA TGGCAAGGAA GTTCTCTGCA
    CCTGGGCAAC TGTGCATCTC CATGACCTCG AACCTGGGTG GCTCTGCCCC CATCTCTGCA
    GCATCAGCTA CCTCTCTAGG TCACTTCACC AAGTCTATGT GCCCCCCACA GCAGTATGGC
    TTTCCAGCTA CCCCATTTGG CGCTCAATGG AGTGGGACGG GTGGCCCAGC ACCACAGCCA
    CTTGGCCAGT TCCAACCTGT GGGAACTGCC TCCTTGCAGA ATTTCAACAT CAGCAATTTG
    CAGAAATCCA TCAGCAACCC CCCAGGCTCC AACCTGCGGA CCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011519299.1
    CDS974..9361
    Translation

    Target ORF information:

    RefSeq Version XM_011520997.3
    Organism Homo sapiens(human)
    Definition Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011520997.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG
    TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT
    CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT
    AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT
    GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT
    ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT
    ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA
    GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG
    GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA
    CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT
    CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG
    GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG
    AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG
    AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA
    GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT
    CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA
    GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG
    AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC
    CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC
    GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC
    TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA
    TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA
    ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGCAGGG AATACAGCAG
    ACAGCCCCTC CTCAACAGAC AGTGCAGTAT TCACTTTCAC AGACATCAAC CTCCAGTGAG
    GCCACTACTG CACAGCCAGT GAGTCAGCCT CAAGCTCCAC AAGTCTTGCC TCAAGTATCA
    GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA GTACCAACTA TCCAAGGCGA ACCTCAGATC
    CCAGTTGCGA CACAACCCTC GGTTGTTCCA GTCCACTCTG GTGCTCATTT CCTTCCAGTG
    GGACAGCCGC TCCCTACTCC CTTGCTCCCT CAGTACCCTG TCTCTCAGAT TCCCATATCA
    ACTCCTCATG TGTCTACGGC TCAGACAGGT TTCTCATCCC TTCCCATCAC AATGGCAGCT
    GGCATTACTC AGCCTCTGCT CACGTTGGCT TCATCTGCTA CAACAGCTGC GATCCCGGGG
    GTATCAACTG TGGTTCCTAG TCAGCTTCCA ACCCTTCTGC AGCCTGTGAC TCAGCTGCCA
    AGTCAGGTTC ACCCACAGCT CCTACAACCA GCAGTTCAGT CCATGGGAAT ACCAGCTAAC
    CTTGGACAAG CTGCTGAGGT TCCACTTTCC TCTGGAGATG TTCTGTACCA GGGCTTCCCA
    CCTCGACTGC CACCACAGTA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT
    TCTGTTTGCA TCCATTCTAC AGTCCTATCC CCTCCCATGC CGACAGAAGT ACTGGCTACA
    CCTGGGTACT TTCCCACAGT GGTGCAGCCT TATGTGGAAT CAAATCTTTT AGTTCCTATG
    GGTGGTGTAG GAGGACAGGT TCAAGTGTCC CAGCCAGGAG GGAGTTTAGC ACAAGCCCCC
    ACTACATCCT CCCAGCAAGC AGTTTTGGAG AGTACTCAGG GAGTCTCTCA GGTTGCTCCT
    GCAGAGCCAG TTGCAGTAGC ACAGACCCAA GCTACCCAGC CGACCACTTT GGCTTCCTCT
    GTAGACAGTG CACATTCAGA TGTTGCTTCA GGTATGAGTG ATGGCAATGA GAACGTCCCA
    TCTTCCAGTG GAAGGCATGA AGGAAGAACT ACAAAACGGC ATTACCGAAA ATCTGTAAGG
    AGTCGCTCTC GACATGAAAA AACTTCACGC CCAAAATTAA GAATTTTGAA TGTTTCAAAT
    AAAGGAGACC GAGTAGTAGA ATGTCAATTA GAGACTCATA ATAGGAAAAT GGTTACATTC
    AAATTTGACC TAGATGGTGA CAACCCCGAG GAGATAGCAA CAATTATGGT GAACAATGAC
    TTTATTCTAG CAATAGAGAG AGAGTCGTTT GTGGATCAAG TGCGAGAAAT TATTGAAAAA
    GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGATCA GGGATTGGAG
    AGTCTACAAG GAAAGGATGA CTATGGCTTT TCAGGTTCTC AGAAATTGGA AGGAGAGTTC
    AAACAACCAA TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA
    ACTCAAGTTG TTCATTCTGC GGGAAGGCGG TTTATAGTGA GTCCTGTGCC AGAAAGCCGA
    TTACGAGAAT CAAAAGTTTT CCCCAGTGAA ATAACAGATA CAGTTGCTGC CTCTACAGCT
    CAGAGCCCTG GAATGAACTT GTCTCACTCT GCATCATCCC TTAGTCTACA ACAGGCCTTT
    TCTGAACTTA GACGTGCCCA AATGACAGAA GGACCCAACA CAGCACCTCC AAACTTTAGT
    CATACAGGAC CAACATTTCC AGTAGTACCT CCTTTCTTAA GTAGCATTGC TGGAGTCCCA
    ACCACAGCAG CAGCCACAGC ACCAGTCCCT GCAACAAGCA GCCCTCCTAA TGACATTTCC
    ACATCAGTAA TTCAGTCTGA GGTTACAGTG CCCACTGAAG AGGGGATTGC TGGAGTTGCC
    ACCAGCACAG GTGTGGTAAC TTCAGGTGGT CTCCCCATAC CACCTGTGTC TGAATCACCA
    GTACTTTCCA GCGTAGTTTC AAGTATCACA ATACCTGCAG TTGTCTCAAT ATCTACTACA
    TCCCCGTCAC TTCAAGTCCC CACATCCACA TCTGAGATCG TTGTTTCTAG TACAGCACTG
    TATCCTTCAG TAACAGTTTC AGCAACTTCA GCCTCTGCAG GGGGCAGTAC TGCTACCCCA
    GGTCCTAAGC CTCCAGCTGT AGTATCTCAG CAGGCAGCAG GCAGCACTAC TGTGGGAGCC
    ACATTAACAT CAGTTTCTAC CACCACTTCA TTCCCAAGCA CAGCTTCACA GCTGTGCATT
    CAGCTTAGCA GCAGTACTTC TACTCCTACT TTAGCTGAAA CCGTGGTAGT TAGCGCACAC
    TCACTAGATA AGACATCTCA TAGCAGTACA ACTGGATTGG CTTTCTCCCT CTCTGCACCA
    TCTTCCTCTT CCTCTCCTGG AGCAGGAGTG TCTAGTTATA TTTCTCAGCC TGGTGGGCTG
    CATCCTTTGG TCATTCCATC AGTGATAGCT TCTACTCCTA TTCTTCCCCA AGCAGCAGGA
    CCTACTTCTA CACCTTTATT ACCCCAAGTA CCTAGTATCC CACCCTTGGT ACAGCCTGTT
    GCCAATGTGC CTGCTGTACA GCAGACACTA ATTCATAGTC AGCCTCAACC AGCTTTGCTT
    CCCAACCAGC CCCATACTCA TTGTCCTGAA GTAGATTCTG ATACACAACC CAAAGCTCCT
    GGAATTGATG ACATAAAGAC TCTAGAAGAA AAGCTGCGGT CTCTGTTCAG TGAACACAGC
    TCATCTGGAG CTCAGCATGC CTCTGTCTCA CTGGAGACCT CACTAGTCAT AGAGAGCACT
    GTCACACCAG GCATCCCAAC TACTGCTGTT GCACCAAGCA AACTCCTGAC TTCTACCACA
    AGTACTTGCT TACCACCAAC CAATTTACCA CTAGGAACAG TTGCTTTGCC AGTTACACCA
    GTGGTCACAC CTGGGCAAGT TTCTACCCCA GTCAGCACTA CTACATCAGG AGTGAAACCT
    GGAACTGCTC CCTCCAAGCC ACCTCTAACT AAGGCTCCGG TGCTGCCAGT GGGTACTGAA
    CTTCCAGCAG GTACTCTACC CAGCGAGCAG CTGCCACCTT TTCCAGGACC TTCTCTAACC
    CAGTCCCAGC AACCTCTAGA GGATCTTGAT GCTCAATTGA GAAGAACACT TAGTCCAGAG
    ATGATCACAG TGACTTCTGC GGTTGGTCCT GTGTCCATGG CGGCTCCAAC AGCAATCACA
    GAAGCAGGAA CACAGCCTCA GAAGGGTGTT TCTCAAGTCA AAGAAGGCCC TGTCCTAGCA
    ACTAGTTCAG GAGCTGGTGT TTTTAAGATG GGACGATTTC AGGTTTCTGT TGCAGCAGAC
    GGTGCCCAGA AAGAGGGTAA AAATAAGTCA GAAGATGCAA AGTCTGTTCA TTTTGAATCC
    AGCACCTCAG AGTCCTCAGT GCTATCAAGT AGTAGTCCAG AGAGTACCTT GGTGAAACCA
    GAGCCGAATG GCATAACCAT CCCTGGTATC TCTTCAGATG TGCCAGAGAG TGCCCACAAA
    ACTACTGCCT CAGAGGCAAA GTCAGACACT GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG
    GTGACAACTA CAGCAAACAA AGTGGGTCGT TTCTCTGTAT CAAAAACTGA GGACAAGATC
    ACTGACACAA AGAAAGAAGG ACCAGTGGCA TCTCCTCCTT TTATGGATTT GGAACAAGCT
    GTTCTTCCTG CTGTGATACC AAAGAAAGAG AAGCCTGAAC TGTCAGAGCC TTCACATCTA
    AATGGGCCGT CTTCTGACCC GGAGGCCGCT TTTTTAAGTA GGGATGTGGA TGATGGTTCC
    GGTAGTCCAC ACTCGCCCCA TCAGCTGAGC TCAAAGAGCC TTCCTAGCCA GAATCTAAGT
    CAAAGCCTTA GTAATTCATT TAACTCCTCT TACATGAGTA GCGACAATGA GTCAGATATC
    GAAGATGAAG ACTTAAAGTT AGAGCTGCGA CGACTACGAG ATAAACATCT CAAAGAGATT
    CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACCAA ACTGGGCAAG
    GTGCCCCCTG CTGTTATTAT TCCCCCAGCT GCTCCCCTTT CAGGGAGAAG ACGACGACCC
    ACTAAAAGCA AAGGCAGCAA ATCTAGTCGA AGCAGTTCCT TGGGGAATAA AAGCCCCCAG
    CTTTCAGGTA ACCTGTCTGG TCAGAGTGCA GCTTCAGTCT TGCACCCCCA GCAGACCCTC
    CACCCTCCTG GCAACATCCC AGAGTCCGGG CAGAATCAGC TGTTACAGCC CCTTAAGCCA
    TCTCCCTCCA GTGACAACCT CTATTCAGCC TTCACCAGTG ATGGTGCCAT TTCAGTACCA
    AGCCTTTCTG CTCCAGGTCA AGGAACCAGC AGCACAAACA CTGTTGGGGC AACAGTGAAC
    AGCCAAGCCG CCCAAGCTCA GCCTCCTGCC ATGACGTCCA GCAGGAAGGG CACATTCACA
    GATGACTTGC ACAAGTTGGT AGACAATTGG GCCCGAGATG CCATGAATCT CTCAGGCAGG
    AGAGGAAGCA AAGGGCACAT GAATTACGAG GGCCCTGGAA TGGCAAGGAA GTTCTCTGCA
    CCTGGGCAAC TGTGCATCTC CATGACCTCG AACCTGGGTG GCTCTGCCCC CATCTCTGCA
    GCATCAGCTA CCTCTCTAGG TCACTTCACC AAGTCTATGT GCCCCCCACA GCAGTATGGC
    TTTCCAGCTA CCCCATTTGG CGCTCAATGG AGTGGGACGG GTGGCCCAGC ACCACAGCCA
    CTTGGCCAGT TCCAACCTGT GGGAACTGCC TCCTTGCAGA ATTTCAACAT CAGCAATTTG
    CAGAAATCCA TCAGCAACCC CCCAGGCTCC AACCTGCGGA CCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71614
    Clone ID Related Accession (Same CDS sequence) XM_011520998.2
    Accession Version XM_011520998.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8385bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase WNK1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011520998.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG
    TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT
    CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT
    AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT
    GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT
    ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT
    ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA
    GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG
    GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA
    CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT
    CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG
    GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG
    AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG
    AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA
    GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT
    CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA
    GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG
    AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC
    CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC
    GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC
    TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA
    TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA
    ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGGGAAT ACAGCAGACA
    GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC
    ACTACTGCAC AGCCAGTGAG TCAGCCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT
    GGAAAACAGC TTCCAGTTTC CCAGCCAGTA CCAACTATCC AAGGCGAACC TCAGATCCCA
    GTTGCGACAC AACCCTCGGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT TCCAGTGGGA
    CAGCCGCTCC CTACTCCCTT GCTCCCTCAG TACCCTGTCT CTCAGATTCC CATATCAACT
    CCTCATGTGT CTACGGCTCA GACAGGTTTC TCATCCCTTC CCATCACAAT GGCAGCTGGC
    ATTACTCAGC CTCTGCTCAC GTTGGCTTCA TCTGCTACAA CAGCTGCGAT CCCGGGGGTA
    TCAACTGTGG TTCCTAGTCA GCTTCCAACC CTTCTGCAGC CTGTGACTCA GCTGCCAAGT
    CAGGTTCACC CACAGCTCCT ACAACCAGCA GTTCAGTCCA TGGGAATACC AGCTAACCTT
    GGACAAGCTG CTGAGGTTCC ACTTTCCTCT GGAGATGTTC TGTACCAGGG CTTCCCACCT
    CGACTGCCAC CACAGTACCC AGGAGATTCA AATATTGCTC CCTCTTCCAA CGTGGCTTCT
    GTTTGCATCC ATTCTACAGT CCTATCCCCT CCCATGCCGA CAGAAGTACT GGCTACACCT
    GGGTACTTTC CCACAGTGGT GCAGCCTTAT GTGGAATCAA ATCTTTTAGT TCCTATGGGT
    GGTGTAGGAG GACAGGTTCA AGTGTCCCAG CCAGGAGGGA GTTTAGCACA AGCCCCCACT
    ACATCCTCCC AGCAAGCAGT TTTGGAGAGT ACTCAGGGAG TCTCTCAGGT TGCTCCTGCA
    GAGCCAGTTG CAGTAGCACA GACCCAAGCT ACCCAGCCGA CCACTTTGGC TTCCTCTGTA
    GACAGTGCAC ATTCAGATGT TGCTTCAGGT ATGAGTGATG GCAATGAGAA CGTCCCATCT
    TCCAGTGGAA GGCATGAAGG AAGAACTACA AAACGGCATT ACCGAAAATC TGTAAGGAGT
    CGCTCTCGAC ATGAAAAAAC TTCACGCCCA AAATTAAGAA TTTTGAATGT TTCAAATAAA
    GGAGACCGAG TAGTAGAATG TCAATTAGAG ACTCATAATA GGAAAATGGT TACATTCAAA
    TTTGACCTAG ATGGTGACAA CCCCGAGGAG ATAGCAACAA TTATGGTGAA CAATGACTTT
    ATTCTAGCAA TAGAGAGAGA GTCGTTTGTG GATCAAGTGC GAGAAATTAT TGAAAAAGCT
    GATGAAATGC TCAGTGAGGA TGTCAGTGTG GAACCAGAGG GTGATCAGGG ATTGGAGAGT
    CTACAAGGAA AGGATGACTA TGGCTTTTCA GGTTCTCAGA AATTGGAAGG AGAGTTCAAA
    CAACCAATTC CTGCGTCTTC CATGCCACAG CAAATAGGCA TTCCTACCAG TTCTTTAACT
    CAAGTTGTTC ATTCTGCGGG AAGGCGGTTT ATAGTGAGTC CTGTGCCAGA AAGCCGATTA
    CGAGAATCAA AAGTTTTCCC CAGTGAAATA ACAGATACAG TTGCTGCCTC TACAGCTCAG
    AGCCCTGGAA TGAACTTGTC TCACTCTGCA TCATCCCTTA GTCTACAACA GGCCTTTTCT
    GAACTTAGAC GTGCCCAAAT GACAGAAGGA CCCAACACAG CACCTCCAAA CTTTAGTCAT
    ACAGGACCAA CATTTCCAGT AGTACCTCCT TTCTTAAGTA GCATTGCTGG AGTCCCAACC
    ACAGCAGCAG CCACAGCACC AGTCCCTGCA ACAAGCAGCC CTCCTAATGA CATTTCCACA
    TCAGTAATTC AGTCTGAGGT TACAGTGCCC ACTGAAGAGG GGATTGCTGG AGTTGCCACC
    AGCACAGGTG TGGTAACTTC AGGTGGTCTC CCCATACCAC CTGTGTCTGA ATCACCAGTA
    CTTTCCAGCG TAGTTTCAAG TATCACAATA CCTGCAGTTG TCTCAATATC TACTACATCC
    CCGTCACTTC AAGTCCCCAC ATCCACATCT GAGATCGTTG TTTCTAGTAC AGCACTGTAT
    CCTTCAGTAA CAGTTTCAGC AACTTCAGCC TCTGCAGGGG GCAGTACTGC TACCCCAGGT
    CCTAAGCCTC CAGCTGTAGT ATCTCAGCAG GCAGCAGGCA GCACTACTGT GGGAGCCACA
    TTAACATCAG TTTCTACCAC CACTTCATTC CCAAGCACAG CTTCACAGCT GTGCATTCAG
    CTTAGCAGCA GTACTTCTAC TCCTACTTTA GCTGAAACCG TGGTAGTTAG CGCACACTCA
    CTAGATAAGA CATCTCATAG CAGTACAACT GGATTGGCTT TCTCCCTCTC TGCACCATCT
    TCCTCTTCCT CTCCTGGAGC AGGAGTGTCT AGTTATATTT CTCAGCCTGG TGGGCTGCAT
    CCTTTGGTCA TTCCATCAGT GATAGCTTCT ACTCCTATTC TTCCCCAAGC AGCAGGACCT
    ACTTCTACAC CTTTATTACC CCAAGTACCT AGTATCCCAC CCTTGGTACA GCCTGTTGCC
    AATGTGCCTG CTGTACAGCA GACACTAATT CATAGTCAGC CTCAACCAGC TTTGCTTCCC
    AACCAGCCCC ATACTCATTG TCCTGAAGTA GATTCTGATA CACAACCCAA AGCTCCTGGA
    ATTGATGACA TAAAGACTCT AGAAGAAAAG CTGCGGTCTC TGTTCAGTGA ACACAGCTCA
    TCTGGAGCTC AGCATGCCTC TGTCTCACTG GAGACCTCAC TAGTCATAGA GAGCACTGTC
    ACACCAGGCA TCCCAACTAC TGCTGTTGCA CCAAGCAAAC TCCTGACTTC TACCACAAGT
    ACTTGCTTAC CACCAACCAA TTTACCACTA GGAACAGTTG CTTTGCCAGT TACACCAGTG
    GTCACACCTG GGCAAGTTTC TACCCCAGTC AGCACTACTA CATCAGGAGT GAAACCTGGA
    ACTGCTCCCT CCAAGCCACC TCTAACTAAG GCTCCGGTGC TGCCAGTGGG TACTGAACTT
    CCAGCAGGTA CTCTACCCAG CGAGCAGCTG CCACCTTTTC CAGGACCTTC TCTAACCCAG
    TCCCAGCAAC CTCTAGAGGA TCTTGATGCT CAATTGAGAA GAACACTTAG TCCAGAGATG
    ATCACAGTGA CTTCTGCGGT TGGTCCTGTG TCCATGGCGG CTCCAACAGC AATCACAGAA
    GCAGGAACAC AGCCTCAGAA GGGTGTTTCT CAAGTCAAAG AAGGCCCTGT CCTAGCAACT
    AGTTCAGGAG CTGGTGTTTT TAAGATGGGA CGATTTCAGG TTTCTGTTGC AGCAGACGGT
    GCCCAGAAAG AGGGTAAAAA TAAGTCAGAA GATGCAAAGT CTGTTCATTT TGAATCCAGC
    ACCTCAGAGT CCTCAGTGCT ATCAAGTAGT AGTCCAGAGA GTACCTTGGT GAAACCAGAG
    CCGAATGGCA TAACCATCCC TGGTATCTCT TCAGATGTGC CAGAGAGTGC CCACAAAACT
    ACTGCCTCAG AGGCAAAGTC AGACACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG
    ACAACTACAG CAAACAAAGT GGGTCGTTTC TCTGTATCAA AAACTGAGGA CAAGATCACT
    GACACAAAGA AAGAAGGACC AGTGGCATCT CCTCCTTTTA TGGATTTGGA ACAAGCTGTT
    CTTCCTGCTG TGATACCAAA GAAAGAGAAG CCTGAACTGT CAGAGCCTTC ACATCTAAAT
    GGGCCGTCTT CTGACCCGGA GGCCGCTTTT TTAAGTAGGG ATGTGGATGA TGGTTCCGGT
    AGTCCACACT CGCCCCATCA GCTGAGCTCA AAGAGCCTTC CTAGCCAGAA TCTAAGTCAA
    AGCCTTAGTA ATTCATTTAA CTCCTCTTAC ATGAGTAGCG ACAATGAGTC AGATATCGAA
    GATGAAGACT TAAAGTTAGA GCTGCGACGA CTACGAGATA AACATCTCAA AGAGATTCAG
    GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGCAAGGTG
    CCCCCTGCTG TTATTATTCC CCCAGCTGCT CCCCTTTCAG GGAGAAGACG ACGACCCACT
    AAAAGCAAAG GCAGCAAATC TAGTCGAAGC AGTTCCTTGG GGAATAAAAG CCCCCAGCTT
    TCAGGTAACC TGTCTGGTCA GAGTGCAGCT TCAGTCTTGC ACCCCCAGCA GACCCTCCAC
    CCTCCTGGCA ACATCCCAGA GTCCGGGCAG AATCAGCTGT TACAGCCCCT TAAGCCATCT
    CCCTCCAGTG ACAACCTCTA TTCAGCCTTC ACCAGTGATG GTGCCATTTC AGTACCAAGC
    CTTTCTGCTC CAGGTCAAGG AACCAGCAGC ACAAACACTG TTGGGGCAAC AGTGAACAGC
    CAAGCCGCCC AAGCTCAGCC TCCTGCCATG ACGTCCAGCA GGAAGGGCAC ATTCACAGAT
    GACTTGCACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGGAGA
    GGAAGCAAAG GGCACATGAA TTACGAGGGC CCTGGAATGG CAAGGAAGTT CTCTGCACCT
    GGGCAACTGT GCATCTCCAT GACCTCGAAC CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA
    TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTATGGCTTT
    CCAGCTACCC CATTTGGCGC TCAATGGAGT GGGACGGGTG GCCCAGCACC ACAGCCACTT
    GGCCAGTTCC AACCTGTGGG AACTGCCTCC TTGCAGAATT TCAACATCAG CAATTTGCAG
    AAATCCATCA GCAACCCCCC AGGCTCCAAC CTGCGGACCA CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011519300.1
    CDS969..9353
    Translation

    Target ORF information:

    RefSeq Version XM_011520998.2
    Organism Homo sapiens(human)
    Definition Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011520998.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG
    TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT
    CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT
    AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT
    GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT
    ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT
    ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA
    GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG
    GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA
    CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT
    CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG
    GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG
    AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG
    AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA
    GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT
    CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA
    GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG
    AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC
    CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC
    GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC
    TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA
    TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA
    ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGGGAAT ACAGCAGACA
    GCCCCTCCTC AACAGACAGT GCAGTATTCA CTTTCACAGA CATCAACCTC CAGTGAGGCC
    ACTACTGCAC AGCCAGTGAG TCAGCCTCAA GCTCCACAAG TCTTGCCTCA AGTATCAGCT
    GGAAAACAGC TTCCAGTTTC CCAGCCAGTA CCAACTATCC AAGGCGAACC TCAGATCCCA
    GTTGCGACAC AACCCTCGGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT TCCAGTGGGA
    CAGCCGCTCC CTACTCCCTT GCTCCCTCAG TACCCTGTCT CTCAGATTCC CATATCAACT
    CCTCATGTGT CTACGGCTCA GACAGGTTTC TCATCCCTTC CCATCACAAT GGCAGCTGGC
    ATTACTCAGC CTCTGCTCAC GTTGGCTTCA TCTGCTACAA CAGCTGCGAT CCCGGGGGTA
    TCAACTGTGG TTCCTAGTCA GCTTCCAACC CTTCTGCAGC CTGTGACTCA GCTGCCAAGT
    CAGGTTCACC CACAGCTCCT ACAACCAGCA GTTCAGTCCA TGGGAATACC AGCTAACCTT
    GGACAAGCTG CTGAGGTTCC ACTTTCCTCT GGAGATGTTC TGTACCAGGG CTTCCCACCT
    CGACTGCCAC CACAGTACCC AGGAGATTCA AATATTGCTC CCTCTTCCAA CGTGGCTTCT
    GTTTGCATCC ATTCTACAGT CCTATCCCCT CCCATGCCGA CAGAAGTACT GGCTACACCT
    GGGTACTTTC CCACAGTGGT GCAGCCTTAT GTGGAATCAA ATCTTTTAGT TCCTATGGGT
    GGTGTAGGAG GACAGGTTCA AGTGTCCCAG CCAGGAGGGA GTTTAGCACA AGCCCCCACT
    ACATCCTCCC AGCAAGCAGT TTTGGAGAGT ACTCAGGGAG TCTCTCAGGT TGCTCCTGCA
    GAGCCAGTTG CAGTAGCACA GACCCAAGCT ACCCAGCCGA CCACTTTGGC TTCCTCTGTA
    GACAGTGCAC ATTCAGATGT TGCTTCAGGT ATGAGTGATG GCAATGAGAA CGTCCCATCT
    TCCAGTGGAA GGCATGAAGG AAGAACTACA AAACGGCATT ACCGAAAATC TGTAAGGAGT
    CGCTCTCGAC ATGAAAAAAC TTCACGCCCA AAATTAAGAA TTTTGAATGT TTCAAATAAA
    GGAGACCGAG TAGTAGAATG TCAATTAGAG ACTCATAATA GGAAAATGGT TACATTCAAA
    TTTGACCTAG ATGGTGACAA CCCCGAGGAG ATAGCAACAA TTATGGTGAA CAATGACTTT
    ATTCTAGCAA TAGAGAGAGA GTCGTTTGTG GATCAAGTGC GAGAAATTAT TGAAAAAGCT
    GATGAAATGC TCAGTGAGGA TGTCAGTGTG GAACCAGAGG GTGATCAGGG ATTGGAGAGT
    CTACAAGGAA AGGATGACTA TGGCTTTTCA GGTTCTCAGA AATTGGAAGG AGAGTTCAAA
    CAACCAATTC CTGCGTCTTC CATGCCACAG CAAATAGGCA TTCCTACCAG TTCTTTAACT
    CAAGTTGTTC ATTCTGCGGG AAGGCGGTTT ATAGTGAGTC CTGTGCCAGA AAGCCGATTA
    CGAGAATCAA AAGTTTTCCC CAGTGAAATA ACAGATACAG TTGCTGCCTC TACAGCTCAG
    AGCCCTGGAA TGAACTTGTC TCACTCTGCA TCATCCCTTA GTCTACAACA GGCCTTTTCT
    GAACTTAGAC GTGCCCAAAT GACAGAAGGA CCCAACACAG CACCTCCAAA CTTTAGTCAT
    ACAGGACCAA CATTTCCAGT AGTACCTCCT TTCTTAAGTA GCATTGCTGG AGTCCCAACC
    ACAGCAGCAG CCACAGCACC AGTCCCTGCA ACAAGCAGCC CTCCTAATGA CATTTCCACA
    TCAGTAATTC AGTCTGAGGT TACAGTGCCC ACTGAAGAGG GGATTGCTGG AGTTGCCACC
    AGCACAGGTG TGGTAACTTC AGGTGGTCTC CCCATACCAC CTGTGTCTGA ATCACCAGTA
    CTTTCCAGCG TAGTTTCAAG TATCACAATA CCTGCAGTTG TCTCAATATC TACTACATCC
    CCGTCACTTC AAGTCCCCAC ATCCACATCT GAGATCGTTG TTTCTAGTAC AGCACTGTAT
    CCTTCAGTAA CAGTTTCAGC AACTTCAGCC TCTGCAGGGG GCAGTACTGC TACCCCAGGT
    CCTAAGCCTC CAGCTGTAGT ATCTCAGCAG GCAGCAGGCA GCACTACTGT GGGAGCCACA
    TTAACATCAG TTTCTACCAC CACTTCATTC CCAAGCACAG CTTCACAGCT GTGCATTCAG
    CTTAGCAGCA GTACTTCTAC TCCTACTTTA GCTGAAACCG TGGTAGTTAG CGCACACTCA
    CTAGATAAGA CATCTCATAG CAGTACAACT GGATTGGCTT TCTCCCTCTC TGCACCATCT
    TCCTCTTCCT CTCCTGGAGC AGGAGTGTCT AGTTATATTT CTCAGCCTGG TGGGCTGCAT
    CCTTTGGTCA TTCCATCAGT GATAGCTTCT ACTCCTATTC TTCCCCAAGC AGCAGGACCT
    ACTTCTACAC CTTTATTACC CCAAGTACCT AGTATCCCAC CCTTGGTACA GCCTGTTGCC
    AATGTGCCTG CTGTACAGCA GACACTAATT CATAGTCAGC CTCAACCAGC TTTGCTTCCC
    AACCAGCCCC ATACTCATTG TCCTGAAGTA GATTCTGATA CACAACCCAA AGCTCCTGGA
    ATTGATGACA TAAAGACTCT AGAAGAAAAG CTGCGGTCTC TGTTCAGTGA ACACAGCTCA
    TCTGGAGCTC AGCATGCCTC TGTCTCACTG GAGACCTCAC TAGTCATAGA GAGCACTGTC
    ACACCAGGCA TCCCAACTAC TGCTGTTGCA CCAAGCAAAC TCCTGACTTC TACCACAAGT
    ACTTGCTTAC CACCAACCAA TTTACCACTA GGAACAGTTG CTTTGCCAGT TACACCAGTG
    GTCACACCTG GGCAAGTTTC TACCCCAGTC AGCACTACTA CATCAGGAGT GAAACCTGGA
    ACTGCTCCCT CCAAGCCACC TCTAACTAAG GCTCCGGTGC TGCCAGTGGG TACTGAACTT
    CCAGCAGGTA CTCTACCCAG CGAGCAGCTG CCACCTTTTC CAGGACCTTC TCTAACCCAG
    TCCCAGCAAC CTCTAGAGGA TCTTGATGCT CAATTGAGAA GAACACTTAG TCCAGAGATG
    ATCACAGTGA CTTCTGCGGT TGGTCCTGTG TCCATGGCGG CTCCAACAGC AATCACAGAA
    GCAGGAACAC AGCCTCAGAA GGGTGTTTCT CAAGTCAAAG AAGGCCCTGT CCTAGCAACT
    AGTTCAGGAG CTGGTGTTTT TAAGATGGGA CGATTTCAGG TTTCTGTTGC AGCAGACGGT
    GCCCAGAAAG AGGGTAAAAA TAAGTCAGAA GATGCAAAGT CTGTTCATTT TGAATCCAGC
    ACCTCAGAGT CCTCAGTGCT ATCAAGTAGT AGTCCAGAGA GTACCTTGGT GAAACCAGAG
    CCGAATGGCA TAACCATCCC TGGTATCTCT TCAGATGTGC CAGAGAGTGC CCACAAAACT
    ACTGCCTCAG AGGCAAAGTC AGACACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG
    ACAACTACAG CAAACAAAGT GGGTCGTTTC TCTGTATCAA AAACTGAGGA CAAGATCACT
    GACACAAAGA AAGAAGGACC AGTGGCATCT CCTCCTTTTA TGGATTTGGA ACAAGCTGTT
    CTTCCTGCTG TGATACCAAA GAAAGAGAAG CCTGAACTGT CAGAGCCTTC ACATCTAAAT
    GGGCCGTCTT CTGACCCGGA GGCCGCTTTT TTAAGTAGGG ATGTGGATGA TGGTTCCGGT
    AGTCCACACT CGCCCCATCA GCTGAGCTCA AAGAGCCTTC CTAGCCAGAA TCTAAGTCAA
    AGCCTTAGTA ATTCATTTAA CTCCTCTTAC ATGAGTAGCG ACAATGAGTC AGATATCGAA
    GATGAAGACT TAAAGTTAGA GCTGCGACGA CTACGAGATA AACATCTCAA AGAGATTCAG
    GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGCAAGGTG
    CCCCCTGCTG TTATTATTCC CCCAGCTGCT CCCCTTTCAG GGAGAAGACG ACGACCCACT
    AAAAGCAAAG GCAGCAAATC TAGTCGAAGC AGTTCCTTGG GGAATAAAAG CCCCCAGCTT
    TCAGGTAACC TGTCTGGTCA GAGTGCAGCT TCAGTCTTGC ACCCCCAGCA GACCCTCCAC
    CCTCCTGGCA ACATCCCAGA GTCCGGGCAG AATCAGCTGT TACAGCCCCT TAAGCCATCT
    CCCTCCAGTG ACAACCTCTA TTCAGCCTTC ACCAGTGATG GTGCCATTTC AGTACCAAGC
    CTTTCTGCTC CAGGTCAAGG AACCAGCAGC ACAAACACTG TTGGGGCAAC AGTGAACAGC
    CAAGCCGCCC AAGCTCAGCC TCCTGCCATG ACGTCCAGCA GGAAGGGCAC ATTCACAGAT
    GACTTGCACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGGAGA
    GGAAGCAAAG GGCACATGAA TTACGAGGGC CCTGGAATGG CAAGGAAGTT CTCTGCACCT
    GGGCAACTGT GCATCTCCAT GACCTCGAAC CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA
    TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTATGGCTTT
    CCAGCTACCC CATTTGGCGC TCAATGGAGT GGGACGGGTG GCCCAGCACC ACAGCCACTT
    GGCCAGTTCC AACCTGTGGG AACTGCCTCC TTGCAGAATT TCAACATCAG CAATTTGCAG
    AAATCCATCA GCAACCCCCC AGGCTCCAAC CTGCGGACCA CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71615
    Clone ID Related Accession (Same CDS sequence) XM_011520999.2
    Accession Version XM_011520999.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8385bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase WNK1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011520999.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG
    TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT
    CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT
    AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT
    GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT
    ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT
    ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA
    GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG
    GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA
    CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT
    CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG
    GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG
    AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG
    AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA
    GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT
    CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA
    GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG
    AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC
    CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC
    GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC
    TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA
    TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA
    ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGCAGGG AATACAGCAG
    ACAGCCCCTC CTCAACAGAC AGTGCAGTAT TCACTTTCAC AGACATCAAC CTCCAGTGAG
    GCCACTACTG CACAGCCAGT GAGTCAGCCT CAAGCTCCAC AAGTCTTGCC TCAAGTATCA
    GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA GTACCAACTA TCCAAGGCGA ACCTCAGATC
    CCAGTTGCGA CACAACCCTC GGTTGTTCCA GTCCACTCTG GTGCTCATTT CCTTCCAGTG
    GGACAGCCGC TCCCTACTCC CTTGCTCCCT CAGTACCCTG TCTCTCAGAT TCCCATATCA
    ACTCCTCATG TGTCTACGGC TCAGACAGGT TTCTCATCCC TTCCCATCAC AATGGCAGCT
    GGCATTACTC AGCCTCTGCT CACGTTGGCT TCATCTGCTA CAACAGCTGC GATCCCGGGG
    GTATCAACTG TGGTTCCTAG TCAGCTTCCA ACCCTTCTGC AGCCTGTGAC TCAGCTGCCA
    AGTCAGGTTC ACCCACAGCT CCTACAACCA GCAGTTCAGT CCATGGGAAT ACCAGCTAAC
    CTTGGACAAG CTGCTGAGGT TCCACTTTCC TCTGGAGATG TTCTGTACCA GGGCTTCCCA
    CCTCGACTGC CACCACAGTA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT
    TCTGTTTGCA TCCATTCTAC AGTCCTATCC CCTCCCATGC CGACAGAAGT ACTGGCTACA
    CCTGGGTACT TTCCCACAGT GGTGCAGCCT TATGTGGAAT CAAATCTTTT AGTTCCTATG
    GGTGGTGTAG GAGGACAGGT TCAAGTGTCC CAGCCAGGAG GGAGTTTAGC ACAAGCCCCC
    ACTACATCCT CCCAGCAAGC AGTTTTGGAG AGTACTCAGG GAGTCTCTCA GGTTGCTCCT
    GCAGAGCCAG TTGCAGTAGC ACAGACCCAA GCTACCCAGC CGACCACTTT GGCTTCCTCT
    GTAGACAGTG CACATTCAGA TGTTGCTTCA GGTATGAGTG ATGGCAATGA GAACGTCCCA
    TCTTCCAGTG GAAGGCATGA AGGAAGAACT ACAAAACGGC ATTACCGAAA ATCTGTAAGG
    AGTCGCTCTC GACATGAAAA AACTTCACGC CCAAAATTAA GAATTTTGAA TGTTTCAAAT
    AAAGGAGACC GAGTAGTAGA ATGTCAATTA GAGACTCATA ATAGGAAAAT GGTTACATTC
    AAATTTGACC TAGATGGTGA CAACCCCGAG GAGATAGCAA CAATTATGGT GAACAATGAC
    TTTATTCTAG CAATAGAGAG AGAGTCGTTT GTGGATCAAG TGCGAGAAAT TATTGAAAAA
    GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGATCA GGGATTGGAG
    AGTCTACAAG GAAAGGATGA CTATGGCTTT TCAGGTTCTC AGAAATTGGA AGGAGAGTTC
    AAACAACCAA TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA
    ACTCAAGTTG TTCATTCTGC GGGAAGGCGG TTTATAGTGA GTCCTGTGCC AGAAAGCCGA
    TTACGAGAAT CAAAAGTTTT CCCCAGTGAA ATAACAGATA CAGTTGCTGC CTCTACAGCT
    CAGAGCCCTG GAATGAACTT GTCTCACTCT GCATCATCCC TTAGTCTACA ACAGGCCTTT
    TCTGAACTTA GACGTGCCCA AATGACAGAA GGACCCAACA CAGCACCTCC AAACTTTAGT
    CATACAGGAC CAACATTTCC AGTAGTACCT CCTTTCTTAA GTAGCATTGC TGGAGTCCCA
    ACCACAGCAG CAGCCACAGC ACCAGTCCCT GCAACAAGCA GCCCTCCTAA TGACATTTCC
    ACATCAGTAA TTCAGTCTGA GGTTACAGTG CCCACTGAAG AGGGGATTGC TGGAGTTGCC
    ACCAGCACAG GTGTGGTAAC TTCAGGTGGT CTCCCCATAC CACCTGTGTC TGAATCACCA
    GTACTTTCCA GCGTAGTTTC AAGTATCACA ATACCTGCAG TTGTCTCAAT ATCTACTACA
    TCCCCGTCAC TTCAAGTCCC CACATCCACA TCTGAGATCG TTGTTTCTAG TACAGCACTG
    TATCCTTCAG TAACAGTTTC AGCAACTTCA GCCTCTGCAG GGGGCAGTAC TGCTACCCCA
    GGTCCTAAGC CTCCAGCTGT AGTATCTCAG CAGGCAGCAG GCAGCACTAC TGTGGGAGCC
    ACATTAACAT CAGTTTCTAC CACCACTTCA TTCCCAAGCA CAGCTTCACA GCTGTGCATT
    CAGCTTAGCA GCAGTACTTC TACTCCTACT TTAGCTGAAA CCGTGGTAGT TAGCGCACAC
    TCACTAGATA AGACATCTCA TAGCAGTACA ACTGGATTGG CTTTCTCCCT CTCTGCACCA
    TCTTCCTCTT CCTCTCCTGG AGCAGGAGTG TCTAGTTATA TTTCTCAGCC TGGTGGGCTG
    CATCCTTTGG TCATTCCATC AGTGATAGCT TCTACTCCTA TTCTTCCCCA AGCAGCAGGA
    CCTACTTCTA CACCTTTATT ACCCCAAGTA CCTAGTATCC CACCCTTGGT ACAGCCTGTT
    GCCAATGTGC CTGCTGTACA GCAGACACTA ATTCATAGTC AGCCTCAACC AGCTTTGCTT
    CCCAACCAGC CCCATACTCA TTGTCCTGAA GTAGATTCTG ATACACAACC CAAAGCTCCT
    GGAATTGATG ACATAAAGAC TCTAGAAGAA AAGCTGCGGT CTCTGTTCAG TGAACACAGC
    TCATCTGGAG CTCAGCATGC CTCTGTCTCA CTGGAGACCT CACTAGTCAT AGAGAGCACT
    GTCACACCAG GCATCCCAAC TACTGCTGTT GCACCAAGCA AACTCCTGAC TTCTACCACA
    AGTACTTGCT TACCACCAAC CAATTTACCA CTAGGAACAG TTGCTTTGCC AGTTACACCA
    GTGGTCACAC CTGGGCAAGT TTCTACCCCA GTCAGCACTA CTACATCAGG AGTGAAACCT
    GGAACTGCTC CCTCCAAGCC ACCTCTAACT AAGGCTCCGG TGCTGCCAGT GGGTACTGAA
    CTTCCAGCAG GTACTCTACC CAGCGAGCAG CTGCCACCTT TTCCAGGACC TTCTCTAACC
    CAGTCCCAGC AACCTCTAGA GGATCTTGAT GCTCAATTGA GAAGAACACT TAGTCCAGAG
    ATGATCACAG TGACTTCTGC GGTTGGTCCT GTGTCCATGG CGGCTCCAAC AGCAATCACA
    GAAGCAGGAA CACAGCCTCA GAAGGTTTCT CAAGTCAAAG AAGGCCCTGT CCTAGCAACT
    AGTTCAGGAG CTGGTGTTTT TAAGATGGGA CGATTTCAGG TTTCTGTTGC AGCAGACGGT
    GCCCAGAAAG AGGGTAAAAA TAAGTCAGAA GATGCAAAGT CTGTTCATTT TGAATCCAGC
    ACCTCAGAGT CCTCAGTGCT ATCAAGTAGT AGTCCAGAGA GTACCTTGGT GAAACCAGAG
    CCGAATGGCA TAACCATCCC TGGTATCTCT TCAGATGTGC CAGAGAGTGC CCACAAAACT
    ACTGCCTCAG AGGCAAAGTC AGACACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG
    ACAACTACAG CAAACAAAGT GGGTCGTTTC TCTGTATCAA AAACTGAGGA CAAGATCACT
    GACACAAAGA AAGAAGGACC AGTGGCATCT CCTCCTTTTA TGGATTTGGA ACAAGCTGTT
    CTTCCTGCTG TGATACCAAA GAAAGAGAAG CCTGAACTGT CAGAGCCTTC ACATCTAAAT
    GGGCCGTCTT CTGACCCGGA GGCCGCTTTT TTAAGTAGGG ATGTGGATGA TGGTTCCGGT
    AGTCCACACT CGCCCCATCA GCTGAGCTCA AAGAGCCTTC CTAGCCAGAA TCTAAGTCAA
    AGCCTTAGTA ATTCATTTAA CTCCTCTTAC ATGAGTAGCG ACAATGAGTC AGATATCGAA
    GATGAAGACT TAAAGTTAGA GCTGCGACGA CTACGAGATA AACATCTCAA AGAGATTCAG
    GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGCAAGGTG
    CCCCCTGCTG TTATTATTCC CCCAGCTGCT CCCCTTTCAG GGAGAAGACG ACGACCCACT
    AAAAGCAAAG GCAGCAAATC TAGTCGAAGC AGTTCCTTGG GGAATAAAAG CCCCCAGCTT
    TCAGGTAACC TGTCTGGTCA GAGTGCAGCT TCAGTCTTGC ACCCCCAGCA GACCCTCCAC
    CCTCCTGGCA ACATCCCAGA GTCCGGGCAG AATCAGCTGT TACAGCCCCT TAAGCCATCT
    CCCTCCAGTG ACAACCTCTA TTCAGCCTTC ACCAGTGATG GTGCCATTTC AGTACCAAGC
    CTTTCTGCTC CAGGTCAAGG AACCAGCAGC ACAAACACTG TTGGGGCAAC AGTGAACAGC
    CAAGCCGCCC AAGCTCAGCC TCCTGCCATG ACGTCCAGCA GGAAGGGCAC ATTCACAGAT
    GACTTGCACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGGAGA
    GGAAGCAAAG GGCACATGAA TTACGAGGGC CCTGGAATGG CAAGGAAGTT CTCTGCACCT
    GGGCAACTGT GCATCTCCAT GACCTCGAAC CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA
    TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTATGGCTTT
    CCAGCTACCC CATTTGGCGC TCAATGGAGT GGGACGGGTG GCCCAGCACC ACAGCCACTT
    GGCCAGTTCC AACCTGTGGG AACTGCCTCC TTGCAGAATT TCAACATCAG CAATTTGCAG
    AAATCCATCA GCAACCCCCC AGGCTCCAAC CTGCGGACCA CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011519301.1
    CDS969..9353
    Translation

    Target ORF information:

    RefSeq Version XM_011520999.2
    Organism Homo sapiens(human)
    Definition Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011520999.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG
    TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT
    CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT
    AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT
    GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT
    ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT
    ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA
    GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG
    GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA
    CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT
    CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG
    GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG
    AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG
    AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA
    GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT
    CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA
    GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG
    AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC
    CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC
    GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC
    TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA
    TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA
    ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGCAGGG AATACAGCAG
    ACAGCCCCTC CTCAACAGAC AGTGCAGTAT TCACTTTCAC AGACATCAAC CTCCAGTGAG
    GCCACTACTG CACAGCCAGT GAGTCAGCCT CAAGCTCCAC AAGTCTTGCC TCAAGTATCA
    GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA GTACCAACTA TCCAAGGCGA ACCTCAGATC
    CCAGTTGCGA CACAACCCTC GGTTGTTCCA GTCCACTCTG GTGCTCATTT CCTTCCAGTG
    GGACAGCCGC TCCCTACTCC CTTGCTCCCT CAGTACCCTG TCTCTCAGAT TCCCATATCA
    ACTCCTCATG TGTCTACGGC TCAGACAGGT TTCTCATCCC TTCCCATCAC AATGGCAGCT
    GGCATTACTC AGCCTCTGCT CACGTTGGCT TCATCTGCTA CAACAGCTGC GATCCCGGGG
    GTATCAACTG TGGTTCCTAG TCAGCTTCCA ACCCTTCTGC AGCCTGTGAC TCAGCTGCCA
    AGTCAGGTTC ACCCACAGCT CCTACAACCA GCAGTTCAGT CCATGGGAAT ACCAGCTAAC
    CTTGGACAAG CTGCTGAGGT TCCACTTTCC TCTGGAGATG TTCTGTACCA GGGCTTCCCA
    CCTCGACTGC CACCACAGTA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT
    TCTGTTTGCA TCCATTCTAC AGTCCTATCC CCTCCCATGC CGACAGAAGT ACTGGCTACA
    CCTGGGTACT TTCCCACAGT GGTGCAGCCT TATGTGGAAT CAAATCTTTT AGTTCCTATG
    GGTGGTGTAG GAGGACAGGT TCAAGTGTCC CAGCCAGGAG GGAGTTTAGC ACAAGCCCCC
    ACTACATCCT CCCAGCAAGC AGTTTTGGAG AGTACTCAGG GAGTCTCTCA GGTTGCTCCT
    GCAGAGCCAG TTGCAGTAGC ACAGACCCAA GCTACCCAGC CGACCACTTT GGCTTCCTCT
    GTAGACAGTG CACATTCAGA TGTTGCTTCA GGTATGAGTG ATGGCAATGA GAACGTCCCA
    TCTTCCAGTG GAAGGCATGA AGGAAGAACT ACAAAACGGC ATTACCGAAA ATCTGTAAGG
    AGTCGCTCTC GACATGAAAA AACTTCACGC CCAAAATTAA GAATTTTGAA TGTTTCAAAT
    AAAGGAGACC GAGTAGTAGA ATGTCAATTA GAGACTCATA ATAGGAAAAT GGTTACATTC
    AAATTTGACC TAGATGGTGA CAACCCCGAG GAGATAGCAA CAATTATGGT GAACAATGAC
    TTTATTCTAG CAATAGAGAG AGAGTCGTTT GTGGATCAAG TGCGAGAAAT TATTGAAAAA
    GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGATCA GGGATTGGAG
    AGTCTACAAG GAAAGGATGA CTATGGCTTT TCAGGTTCTC AGAAATTGGA AGGAGAGTTC
    AAACAACCAA TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA
    ACTCAAGTTG TTCATTCTGC GGGAAGGCGG TTTATAGTGA GTCCTGTGCC AGAAAGCCGA
    TTACGAGAAT CAAAAGTTTT CCCCAGTGAA ATAACAGATA CAGTTGCTGC CTCTACAGCT
    CAGAGCCCTG GAATGAACTT GTCTCACTCT GCATCATCCC TTAGTCTACA ACAGGCCTTT
    TCTGAACTTA GACGTGCCCA AATGACAGAA GGACCCAACA CAGCACCTCC AAACTTTAGT
    CATACAGGAC CAACATTTCC AGTAGTACCT CCTTTCTTAA GTAGCATTGC TGGAGTCCCA
    ACCACAGCAG CAGCCACAGC ACCAGTCCCT GCAACAAGCA GCCCTCCTAA TGACATTTCC
    ACATCAGTAA TTCAGTCTGA GGTTACAGTG CCCACTGAAG AGGGGATTGC TGGAGTTGCC
    ACCAGCACAG GTGTGGTAAC TTCAGGTGGT CTCCCCATAC CACCTGTGTC TGAATCACCA
    GTACTTTCCA GCGTAGTTTC AAGTATCACA ATACCTGCAG TTGTCTCAAT ATCTACTACA
    TCCCCGTCAC TTCAAGTCCC CACATCCACA TCTGAGATCG TTGTTTCTAG TACAGCACTG
    TATCCTTCAG TAACAGTTTC AGCAACTTCA GCCTCTGCAG GGGGCAGTAC TGCTACCCCA
    GGTCCTAAGC CTCCAGCTGT AGTATCTCAG CAGGCAGCAG GCAGCACTAC TGTGGGAGCC
    ACATTAACAT CAGTTTCTAC CACCACTTCA TTCCCAAGCA CAGCTTCACA GCTGTGCATT
    CAGCTTAGCA GCAGTACTTC TACTCCTACT TTAGCTGAAA CCGTGGTAGT TAGCGCACAC
    TCACTAGATA AGACATCTCA TAGCAGTACA ACTGGATTGG CTTTCTCCCT CTCTGCACCA
    TCTTCCTCTT CCTCTCCTGG AGCAGGAGTG TCTAGTTATA TTTCTCAGCC TGGTGGGCTG
    CATCCTTTGG TCATTCCATC AGTGATAGCT TCTACTCCTA TTCTTCCCCA AGCAGCAGGA
    CCTACTTCTA CACCTTTATT ACCCCAAGTA CCTAGTATCC CACCCTTGGT ACAGCCTGTT
    GCCAATGTGC CTGCTGTACA GCAGACACTA ATTCATAGTC AGCCTCAACC AGCTTTGCTT
    CCCAACCAGC CCCATACTCA TTGTCCTGAA GTAGATTCTG ATACACAACC CAAAGCTCCT
    GGAATTGATG ACATAAAGAC TCTAGAAGAA AAGCTGCGGT CTCTGTTCAG TGAACACAGC
    TCATCTGGAG CTCAGCATGC CTCTGTCTCA CTGGAGACCT CACTAGTCAT AGAGAGCACT
    GTCACACCAG GCATCCCAAC TACTGCTGTT GCACCAAGCA AACTCCTGAC TTCTACCACA
    AGTACTTGCT TACCACCAAC CAATTTACCA CTAGGAACAG TTGCTTTGCC AGTTACACCA
    GTGGTCACAC CTGGGCAAGT TTCTACCCCA GTCAGCACTA CTACATCAGG AGTGAAACCT
    GGAACTGCTC CCTCCAAGCC ACCTCTAACT AAGGCTCCGG TGCTGCCAGT GGGTACTGAA
    CTTCCAGCAG GTACTCTACC CAGCGAGCAG CTGCCACCTT TTCCAGGACC TTCTCTAACC
    CAGTCCCAGC AACCTCTAGA GGATCTTGAT GCTCAATTGA GAAGAACACT TAGTCCAGAG
    ATGATCACAG TGACTTCTGC GGTTGGTCCT GTGTCCATGG CGGCTCCAAC AGCAATCACA
    GAAGCAGGAA CACAGCCTCA GAAGGTTTCT CAAGTCAAAG AAGGCCCTGT CCTAGCAACT
    AGTTCAGGAG CTGGTGTTTT TAAGATGGGA CGATTTCAGG TTTCTGTTGC AGCAGACGGT
    GCCCAGAAAG AGGGTAAAAA TAAGTCAGAA GATGCAAAGT CTGTTCATTT TGAATCCAGC
    ACCTCAGAGT CCTCAGTGCT ATCAAGTAGT AGTCCAGAGA GTACCTTGGT GAAACCAGAG
    CCGAATGGCA TAACCATCCC TGGTATCTCT TCAGATGTGC CAGAGAGTGC CCACAAAACT
    ACTGCCTCAG AGGCAAAGTC AGACACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG
    ACAACTACAG CAAACAAAGT GGGTCGTTTC TCTGTATCAA AAACTGAGGA CAAGATCACT
    GACACAAAGA AAGAAGGACC AGTGGCATCT CCTCCTTTTA TGGATTTGGA ACAAGCTGTT
    CTTCCTGCTG TGATACCAAA GAAAGAGAAG CCTGAACTGT CAGAGCCTTC ACATCTAAAT
    GGGCCGTCTT CTGACCCGGA GGCCGCTTTT TTAAGTAGGG ATGTGGATGA TGGTTCCGGT
    AGTCCACACT CGCCCCATCA GCTGAGCTCA AAGAGCCTTC CTAGCCAGAA TCTAAGTCAA
    AGCCTTAGTA ATTCATTTAA CTCCTCTTAC ATGAGTAGCG ACAATGAGTC AGATATCGAA
    GATGAAGACT TAAAGTTAGA GCTGCGACGA CTACGAGATA AACATCTCAA AGAGATTCAG
    GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGCAAGGTG
    CCCCCTGCTG TTATTATTCC CCCAGCTGCT CCCCTTTCAG GGAGAAGACG ACGACCCACT
    AAAAGCAAAG GCAGCAAATC TAGTCGAAGC AGTTCCTTGG GGAATAAAAG CCCCCAGCTT
    TCAGGTAACC TGTCTGGTCA GAGTGCAGCT TCAGTCTTGC ACCCCCAGCA GACCCTCCAC
    CCTCCTGGCA ACATCCCAGA GTCCGGGCAG AATCAGCTGT TACAGCCCCT TAAGCCATCT
    CCCTCCAGTG ACAACCTCTA TTCAGCCTTC ACCAGTGATG GTGCCATTTC AGTACCAAGC
    CTTTCTGCTC CAGGTCAAGG AACCAGCAGC ACAAACACTG TTGGGGCAAC AGTGAACAGC
    CAAGCCGCCC AAGCTCAGCC TCCTGCCATG ACGTCCAGCA GGAAGGGCAC ATTCACAGAT
    GACTTGCACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGGAGA
    GGAAGCAAAG GGCACATGAA TTACGAGGGC CCTGGAATGG CAAGGAAGTT CTCTGCACCT
    GGGCAACTGT GCATCTCCAT GACCTCGAAC CTGGGTGGCT CTGCCCCCAT CTCTGCAGCA
    TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTATGGCTTT
    CCAGCTACCC CATTTGGCGC TCAATGGAGT GGGACGGGTG GCCCAGCACC ACAGCCACTT
    GGCCAGTTCC AACCTGTGGG AACTGCCTCC TTGCAGAATT TCAACATCAG CAATTTGCAG
    AAATCCATCA GCAACCCCCC AGGCTCCAAC CTGCGGACCA CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71616
    Clone ID Related Accession (Same CDS sequence) XM_011521000.2
    Accession Version XM_011521000.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8193bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase WNK1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521000.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
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    541
    601
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    721
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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
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    2221
    2281
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    2641
    2701
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    2941
    3001
    3061
    3121
    3181
    3241
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    4141
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    4441
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    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
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    6721
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    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG
    TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT
    CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT
    AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT
    GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT
    ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT
    ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA
    GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG
    GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA
    CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT
    CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG
    GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG
    AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG
    AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA
    GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT
    CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA
    GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG
    AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC
    CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC
    GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC
    TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA
    TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA
    ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGCAGGG AATACAGCAG
    ACAGCCCCTC CTCAACAGAC AGTGCAGTAT TCACTTTCAC AGACATCAAC CTCCAGTGAG
    GCCACTACTG CACAGCCAGT GAGTCAGCCT CAAGCTCCAC AAGTCTTGCC TCAAGTATCA
    GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA GTACCAACTA TCCAAGGCGA ACCTCAGATC
    CCAGTTGCGA CACAACCCTC GGTTGTTCCA GTCCACTCTG GTGCTCATTT CCTTCCAGTG
    GGACAGCCGC TCCCTACTCC CTTGCTCCCT CAGTACCCTG TCTCTCAGAT TCCCATATCA
    ACTCCTCATG TGTCTACGGC TCAGACAGGT TTCTCATCCC TTCCCATCAC AATGGCAGCT
    GGCATTACTC AGCCTCTGCT CACGTTGGCT TCATCTGCTA CAACAGCTGC GATCCCGGGG
    GTATCAACTG TGGTTCCTAG TCAGCTTCCA ACCCTTCTGC AGCCTGTGAC TCAGCTGCCA
    AGTCAGGTTC ACCCACAGCT CCTACAACCA GCAGTTCAGT CCATGGGAAT ACCAGCTAAC
    CTTGGACAAG CTGCTGAGGT TCCACTTTCC TCTGGAGATG TTCTGTACCA GGGCTTCCCA
    CCTCGACTGC CACCACAGTA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT
    TCTGTTTGCA TCCATTCTAC AGTCCTATCC CCTCCCATGC CGACAGAAGT ACTGGCTACA
    CCTGGGTACT TTCCCACAGT GGTGCAGCCT TATGTGGAAT CAAATCTTTT AGTTCCTATG
    GGTGGTGTAG GAGGACAGGT TCAAGTGTCC CAGCCAGGAG GGAGTTTAGC ACAAGCCCCC
    ACTACATCCT CCCAGCAAGC AGTTTTGGAG AGTACTCAGG GAGTCTCTCA GGTTGCTCCT
    GCAGAGCCAG TTGCAGTAGC ACAGACCCAA GCTACCCAGC CGACCACTTT GGCTTCCTCT
    GTAGACAGTG CACATTCAGA TGTTGCTTCA GGTATGAGTG ATGGCAATGA GAACGTCCCA
    TCTTCCAGTG GAAGGCATGA AGGAAGAACT ACAAAACGGC ATTACCGAAA ATCTGTAAGG
    AGTCGCTCTC GACATGAAAA AACTTCACGC CCAAAATTAA GAATTTTGAA TGTTTCAAAT
    AAAGGAGACC GAGTAGTAGA ATGTCAATTA GAGACTCATA ATAGGAAAAT GGTTACATTC
    AAATTTGACC TAGATGGTGA CAACCCCGAG GAGATAGCAA CAATTATGGT GAACAATGAC
    TTTATTCTAG CAATAGAGAG AGAGTCGTTT GTGGATCAAG TGCGAGAAAT TATTGAAAAA
    GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGATCA GGGATTGGAG
    AGTCTACAAG GAAAGGATGA CTATGGCTTT TCAGGTTCTC AGAAATTGGA AGGAGAGTTC
    AAACAACCAA TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA
    ACTCAAGTTG TTCATTCTGC GGGAAGGCGG TTTATAGTGA GTCCTGTGCC AGAAAGCCGA
    TTACGAGAAT CAAAAGTTTT CCCCAGTGAA ATAACAGATA CAGTTGCTGC CTCTACAGCT
    CAGAGCCCTG GAATGAACTT GTCTCACTCT GCATCATCCC TTAGTCTACA ACAGGCCTTT
    TCTGAACTTA GACGTGCCCA AATGACAGAA GGACCCAACA CAGCACCTCC AAACTTTAGT
    CATACAGGAC CAACATTTCC AGTAGTACCT CCTTTCTTAA GTAGCATTGC TGGAGTCCCA
    ACCACAGCAG CAGCCACAGC ACCAGTCCCT GCAACAAGCA GCCCTCCTAA TGACATTTCC
    ACATCAGTAA TTCAGTCTGA GGTTACAGTG CCCACTGAAG AGGGGATTGC TGGAGTTGCC
    ACCAGCACAG GTGTGGTAAC TTCAGGTGGT CTCCCCATAC CACCTGTGTC TGAATCACCA
    GTACTTTCCA GCGTAGTTTC AAGTATCACA ATACCTGCAG TTGTCTCAAT ATCTACTACA
    TCCCCGTCAC TTCAAGTCCC CACATCCACA TCTGAGATCG TTGTTTCTAG TACAGCACTG
    TATCCTTCAG TAACAGTTTC AGCAACTTCA GCCTCTGCAG GGGGCAGTAC TGCTACCCCA
    GGTCCTAAGC CTCCAGCTGT AGTATCTCAG CAGGCAGCAG GCAGCACTAC TGTGGGAGCC
    ACATTAACAT CAGTTTCTAC CACCACTTCA TTCCCAAGCA CAGCTTCACA GCTGTGCATT
    CAGCTTAGCA GCAGTACTTC TACTCCTACT TTAGCTGAAA CCGTGGTAGT TAGCGCACAC
    TCACTAGATA AGACATCTCA TAGCAGTACA ACTGGATTGG CTTTCTCCCT CTCTGCACCA
    TCTTCCTCTT CCTCTCCTGG AGCAGGAGTG TCTAGTTATA TTTCTCAGCC TGGTGGGCTG
    CATCCTTTGG TCATTCCATC AGTGATAGCT TCTACTCCTA TTCTTCCCCA AGCAGCAGGA
    CCTACTTCTA CACCTTTATT ACCCCAAGTA CCTAGTATCC CACCCTTGGT ACAGCCTGTT
    GCCAATGTGC CTGCTGTACA GCAGACACTA ATTCATAGTC AGCCTCAACC AGCTTTGCTT
    CCCAACCAGC CCCATACTCA TTGTCCTGAA GTAGATTCTG ATACACAACC CAAAGCTCCT
    GGAATTGATG ACATAAAGAC TCTAGAAGAA AAGCTGCGGT CTCTGTTCAG TGAACACAGC
    TCATCTGGAG CTCAGCATGC CTCTGTCTCA CTGGAGACCT CACTAGTCAT AGAGAGCACT
    GTCACACCAG GCATCCCAAC TACTGCTGTT GCACCAAGCA AACTCCTGAC TTCTACCACA
    AGTACTTGCT TACCACCAAC CAATTTACCA CTAGGAACAG TTGCTTTGCC AGTTACACCA
    GTGGTCACAC CTGGGCAAGT TTCTACCCCA GTCAGCACTA CTACATCAGG AGTGAAACCT
    GGAACTGCTC CCTCCAAGCC ACCTCTAACT AAGGCTCCGG TGCTGCCAGT GGGTACTGAA
    CTTCCAGCAG GTACTCTACC CAGCGAGCAG CTGCCACCTT TTCCAGGACC TTCTCTAACC
    CAGTCCCAGC AACCTCTAGA GGATCTTGAT GCTCAATTGA GAAGAACACT TAGTCCAGAG
    ATGATCACAG TGACTTCTGC GGTTGGTCCT GTGTCCATGG CGGCTCCAAC AGCAATCACA
    GAAGCAGGAA CACAGCCTCA GAAGGGTGTT TCTCAAGTCA AAGAAGGCCC TGTCCTAGCA
    ACTAGTTCAG GAGCTGGTGT TTTTAAGATG GGACGATTTC AGGTTTCTGT TGCAGCAGAC
    GGTGCCCAGA AAGAGGGTAA AAATAAGTCA GAAGATGCAA AGTCTGTTCA TTTTGAATCC
    AGCACCTCAG AGTCCTCAGT GCTATCAAGT AGTAGTCCAG AGAGTACCTT GGTGAAACCA
    GAGCCGAATG GCATAACCAT CCCTGGTATC TCTTCAGATG TGCCAGAGAG TGCCCACAAA
    ACTACTGCCT CAGAGGCAAA GTCAGACACT GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG
    GTGACAACTA CAGCAAACAA AGTGGGTCGT TTCTCTGTAT CAAAAACTGA GGACAAGATC
    ACTGACACAA AGAAAGAAGG ACCAGTGGCA TCTCCTCCTT TTATGGATTT GGAACAAGCT
    GTTCTTCCTG CTGTGATACC AAAGAAAGAG AAGCCTGAAC TGTCAGAGCC TTCACATCTA
    AATGGGCCGT CTTCTGACCC GGAGGCCGCT TTTTTAAGTA GGGATGTGGA TGATGGTTCC
    GGTAGTCCAC ACTCGCCCCA TCAGCTGAGC TCAAAGAGCC TTCCTAGCCA GAATCTAAGT
    CAAAGCCTTA GTAATTCATT TAACTCCTCT TACATGAGTA GCGACAATGA GTCAGATATC
    GAAGATGAAG ACTTAAAGTT AGAGCTGCGA CGACTACGAG ATAAACATCT CAAAGAGATT
    CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACCAA ACTGGGCAAG
    GTGCCCCCTG CTGTTATTAT TCCCCCAGCT GCTCCCCTTT CAGGGAGAAG ACGACGACCC
    ACTAAAAGCA AAGGCAGCAA ATCTAGTCGA AGCAGTTCCT TGGGGAATAA AAGCCCCCAG
    CTTTCAGGAA CCAGCAGCAC AAACACTGTT GGGGCAACAG TGAACAGCCA AGCCGCCCAA
    GCTCAGCCTC CTGCCATGAC GTCCAGCAGG AAGGGCACAT TCACAGATGA CTTGCACAAG
    TTGGTAGACA ATTGGGCCCG AGATGCCATG AATCTCTCAG GCAGGAGAGG AAGCAAAGGG
    CACATGAATT ACGAGGGCCC TGGAATGGCA AGGAAGTTCT CTGCACCTGG GCAACTGTGC
    ATCTCCATGA CCTCGAACCT GGGTGGCTCT GCCCCCATCT CTGCAGCATC AGCTACCTCT
    CTAGGTCACT TCACCAAGTC TATGTGCCCC CCACAGCAGT ATGGCTTTCC AGCTACCCCA
    TTTGGCGCTC AATGGAGTGG GACGGGTGGC CCAGCACCAC AGCCACTTGG CCAGTTCCAA
    CCTGTGGGAA CTGCCTCCTT GCAGAATTTC AACATCAGCA ATTTGCAGAA ATCCATCAGC
    AACCCCCCAG GCTCCAACCT GCGGACCACT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011519302.1
    CDS969..9161
    Translation

    Target ORF information:

    RefSeq Version XM_011521000.2
    Organism Homo sapiens(human)
    Definition Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011521000.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG
    TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT
    CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT
    AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT
    GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT
    ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT
    ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA
    GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG
    GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA
    CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT
    CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG
    GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG
    AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG
    AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA
    GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT
    CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA
    GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG
    AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC
    CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC
    GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC
    TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA
    TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA
    ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGCAGGG AATACAGCAG
    ACAGCCCCTC CTCAACAGAC AGTGCAGTAT TCACTTTCAC AGACATCAAC CTCCAGTGAG
    GCCACTACTG CACAGCCAGT GAGTCAGCCT CAAGCTCCAC AAGTCTTGCC TCAAGTATCA
    GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA GTACCAACTA TCCAAGGCGA ACCTCAGATC
    CCAGTTGCGA CACAACCCTC GGTTGTTCCA GTCCACTCTG GTGCTCATTT CCTTCCAGTG
    GGACAGCCGC TCCCTACTCC CTTGCTCCCT CAGTACCCTG TCTCTCAGAT TCCCATATCA
    ACTCCTCATG TGTCTACGGC TCAGACAGGT TTCTCATCCC TTCCCATCAC AATGGCAGCT
    GGCATTACTC AGCCTCTGCT CACGTTGGCT TCATCTGCTA CAACAGCTGC GATCCCGGGG
    GTATCAACTG TGGTTCCTAG TCAGCTTCCA ACCCTTCTGC AGCCTGTGAC TCAGCTGCCA
    AGTCAGGTTC ACCCACAGCT CCTACAACCA GCAGTTCAGT CCATGGGAAT ACCAGCTAAC
    CTTGGACAAG CTGCTGAGGT TCCACTTTCC TCTGGAGATG TTCTGTACCA GGGCTTCCCA
    CCTCGACTGC CACCACAGTA CCCAGGAGAT TCAAATATTG CTCCCTCTTC CAACGTGGCT
    TCTGTTTGCA TCCATTCTAC AGTCCTATCC CCTCCCATGC CGACAGAAGT ACTGGCTACA
    CCTGGGTACT TTCCCACAGT GGTGCAGCCT TATGTGGAAT CAAATCTTTT AGTTCCTATG
    GGTGGTGTAG GAGGACAGGT TCAAGTGTCC CAGCCAGGAG GGAGTTTAGC ACAAGCCCCC
    ACTACATCCT CCCAGCAAGC AGTTTTGGAG AGTACTCAGG GAGTCTCTCA GGTTGCTCCT
    GCAGAGCCAG TTGCAGTAGC ACAGACCCAA GCTACCCAGC CGACCACTTT GGCTTCCTCT
    GTAGACAGTG CACATTCAGA TGTTGCTTCA GGTATGAGTG ATGGCAATGA GAACGTCCCA
    TCTTCCAGTG GAAGGCATGA AGGAAGAACT ACAAAACGGC ATTACCGAAA ATCTGTAAGG
    AGTCGCTCTC GACATGAAAA AACTTCACGC CCAAAATTAA GAATTTTGAA TGTTTCAAAT
    AAAGGAGACC GAGTAGTAGA ATGTCAATTA GAGACTCATA ATAGGAAAAT GGTTACATTC
    AAATTTGACC TAGATGGTGA CAACCCCGAG GAGATAGCAA CAATTATGGT GAACAATGAC
    TTTATTCTAG CAATAGAGAG AGAGTCGTTT GTGGATCAAG TGCGAGAAAT TATTGAAAAA
    GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGATCA GGGATTGGAG
    AGTCTACAAG GAAAGGATGA CTATGGCTTT TCAGGTTCTC AGAAATTGGA AGGAGAGTTC
    AAACAACCAA TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA
    ACTCAAGTTG TTCATTCTGC GGGAAGGCGG TTTATAGTGA GTCCTGTGCC AGAAAGCCGA
    TTACGAGAAT CAAAAGTTTT CCCCAGTGAA ATAACAGATA CAGTTGCTGC CTCTACAGCT
    CAGAGCCCTG GAATGAACTT GTCTCACTCT GCATCATCCC TTAGTCTACA ACAGGCCTTT
    TCTGAACTTA GACGTGCCCA AATGACAGAA GGACCCAACA CAGCACCTCC AAACTTTAGT
    CATACAGGAC CAACATTTCC AGTAGTACCT CCTTTCTTAA GTAGCATTGC TGGAGTCCCA
    ACCACAGCAG CAGCCACAGC ACCAGTCCCT GCAACAAGCA GCCCTCCTAA TGACATTTCC
    ACATCAGTAA TTCAGTCTGA GGTTACAGTG CCCACTGAAG AGGGGATTGC TGGAGTTGCC
    ACCAGCACAG GTGTGGTAAC TTCAGGTGGT CTCCCCATAC CACCTGTGTC TGAATCACCA
    GTACTTTCCA GCGTAGTTTC AAGTATCACA ATACCTGCAG TTGTCTCAAT ATCTACTACA
    TCCCCGTCAC TTCAAGTCCC CACATCCACA TCTGAGATCG TTGTTTCTAG TACAGCACTG
    TATCCTTCAG TAACAGTTTC AGCAACTTCA GCCTCTGCAG GGGGCAGTAC TGCTACCCCA
    GGTCCTAAGC CTCCAGCTGT AGTATCTCAG CAGGCAGCAG GCAGCACTAC TGTGGGAGCC
    ACATTAACAT CAGTTTCTAC CACCACTTCA TTCCCAAGCA CAGCTTCACA GCTGTGCATT
    CAGCTTAGCA GCAGTACTTC TACTCCTACT TTAGCTGAAA CCGTGGTAGT TAGCGCACAC
    TCACTAGATA AGACATCTCA TAGCAGTACA ACTGGATTGG CTTTCTCCCT CTCTGCACCA
    TCTTCCTCTT CCTCTCCTGG AGCAGGAGTG TCTAGTTATA TTTCTCAGCC TGGTGGGCTG
    CATCCTTTGG TCATTCCATC AGTGATAGCT TCTACTCCTA TTCTTCCCCA AGCAGCAGGA
    CCTACTTCTA CACCTTTATT ACCCCAAGTA CCTAGTATCC CACCCTTGGT ACAGCCTGTT
    GCCAATGTGC CTGCTGTACA GCAGACACTA ATTCATAGTC AGCCTCAACC AGCTTTGCTT
    CCCAACCAGC CCCATACTCA TTGTCCTGAA GTAGATTCTG ATACACAACC CAAAGCTCCT
    GGAATTGATG ACATAAAGAC TCTAGAAGAA AAGCTGCGGT CTCTGTTCAG TGAACACAGC
    TCATCTGGAG CTCAGCATGC CTCTGTCTCA CTGGAGACCT CACTAGTCAT AGAGAGCACT
    GTCACACCAG GCATCCCAAC TACTGCTGTT GCACCAAGCA AACTCCTGAC TTCTACCACA
    AGTACTTGCT TACCACCAAC CAATTTACCA CTAGGAACAG TTGCTTTGCC AGTTACACCA
    GTGGTCACAC CTGGGCAAGT TTCTACCCCA GTCAGCACTA CTACATCAGG AGTGAAACCT
    GGAACTGCTC CCTCCAAGCC ACCTCTAACT AAGGCTCCGG TGCTGCCAGT GGGTACTGAA
    CTTCCAGCAG GTACTCTACC CAGCGAGCAG CTGCCACCTT TTCCAGGACC TTCTCTAACC
    CAGTCCCAGC AACCTCTAGA GGATCTTGAT GCTCAATTGA GAAGAACACT TAGTCCAGAG
    ATGATCACAG TGACTTCTGC GGTTGGTCCT GTGTCCATGG CGGCTCCAAC AGCAATCACA
    GAAGCAGGAA CACAGCCTCA GAAGGGTGTT TCTCAAGTCA AAGAAGGCCC TGTCCTAGCA
    ACTAGTTCAG GAGCTGGTGT TTTTAAGATG GGACGATTTC AGGTTTCTGT TGCAGCAGAC
    GGTGCCCAGA AAGAGGGTAA AAATAAGTCA GAAGATGCAA AGTCTGTTCA TTTTGAATCC
    AGCACCTCAG AGTCCTCAGT GCTATCAAGT AGTAGTCCAG AGAGTACCTT GGTGAAACCA
    GAGCCGAATG GCATAACCAT CCCTGGTATC TCTTCAGATG TGCCAGAGAG TGCCCACAAA
    ACTACTGCCT CAGAGGCAAA GTCAGACACT GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG
    GTGACAACTA CAGCAAACAA AGTGGGTCGT TTCTCTGTAT CAAAAACTGA GGACAAGATC
    ACTGACACAA AGAAAGAAGG ACCAGTGGCA TCTCCTCCTT TTATGGATTT GGAACAAGCT
    GTTCTTCCTG CTGTGATACC AAAGAAAGAG AAGCCTGAAC TGTCAGAGCC TTCACATCTA
    AATGGGCCGT CTTCTGACCC GGAGGCCGCT TTTTTAAGTA GGGATGTGGA TGATGGTTCC
    GGTAGTCCAC ACTCGCCCCA TCAGCTGAGC TCAAAGAGCC TTCCTAGCCA GAATCTAAGT
    CAAAGCCTTA GTAATTCATT TAACTCCTCT TACATGAGTA GCGACAATGA GTCAGATATC
    GAAGATGAAG ACTTAAAGTT AGAGCTGCGA CGACTACGAG ATAAACATCT CAAAGAGATT
    CAGGACCTGC AGAGTCGCCA GAAGCATGAA ATTGAATCTT TGTATACCAA ACTGGGCAAG
    GTGCCCCCTG CTGTTATTAT TCCCCCAGCT GCTCCCCTTT CAGGGAGAAG ACGACGACCC
    ACTAAAAGCA AAGGCAGCAA ATCTAGTCGA AGCAGTTCCT TGGGGAATAA AAGCCCCCAG
    CTTTCAGGAA CCAGCAGCAC AAACACTGTT GGGGCAACAG TGAACAGCCA AGCCGCCCAA
    GCTCAGCCTC CTGCCATGAC GTCCAGCAGG AAGGGCACAT TCACAGATGA CTTGCACAAG
    TTGGTAGACA ATTGGGCCCG AGATGCCATG AATCTCTCAG GCAGGAGAGG AAGCAAAGGG
    CACATGAATT ACGAGGGCCC TGGAATGGCA AGGAAGTTCT CTGCACCTGG GCAACTGTGC
    ATCTCCATGA CCTCGAACCT GGGTGGCTCT GCCCCCATCT CTGCAGCATC AGCTACCTCT
    CTAGGTCACT TCACCAAGTC TATGTGCCCC CCACAGCAGT ATGGCTTTCC AGCTACCCCA
    TTTGGCGCTC AATGGAGTGG GACGGGTGGC CCAGCACCAC AGCCACTTGG CCAGTTCCAA
    CCTGTGGGAA CTGCCTCCTT GCAGAATTTC AACATCAGCA ATTTGCAGAA ATCCATCAGC
    AACCCCCCAG GCTCCAACCT GCGGACCACT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71617
    Clone ID Related Accession (Same CDS sequence) XM_011521001.2
    Accession Version XM_011521001.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8109bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase WNK1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521001.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG
    TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT
    CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT
    AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT
    GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT
    ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT
    ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA
    GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG
    GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA
    CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT
    CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG
    GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG
    AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG
    AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA
    GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT
    CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA
    GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG
    AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC
    CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC
    GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC
    TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA
    TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA
    ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGCAGGG AATACAGCAG
    ACAGCCCCTC CTCAACAGAC AGTGCAGTAT TCACTTTCAC AGACATCAAC CTCCAGTGAG
    GCCACTACTG CACAGCCAGT GAGTCAGCCT CAAGCTCCAC AAGTCTTGCC TCAAGTATCA
    GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA GTACCAACTA TCCAAGGCGA ACCTCAGATC
    CCAGTTGCGA CACAACCCTC GGTTGTTCCA GTCCACTCTG GTGCTCATTT CCTTCCAGTG
    GGACAGCCGC TCCCTACTCC CTTGCTCCCT CAGTACCCTG TCTCTCAGAT TCCCATATCA
    ACTCCTCATG TGTCTACGGC TCAGACAGGT TTCTCATCCC TTCCCATCAC AATGGCAGCT
    GGCATTACTC AGCCTCTGCT CACGTTGGCT TCATCTGCTA CAACAGCTGC GATCCCGGGG
    GTATCAACTG TGGTTCCTAG TCAGCTTCCA ACCCTTCTGC AGCCTGTGAC TCAGCTGCCA
    AGTCAGGTTC ACCCACAGCT CCTACAACCA GCAGTTCAGT CCATGGGAAT ACCAGCTAAC
    CTTGGACAAG CTGCTGAGGT TCCACTTTCC TCTGGAGATG TTCTGTACCA GAGTACTCAG
    GGAGTCTCTC AGGTTGCTCC TGCAGAGCCA GTTGCAGTAG CACAGACCCA AGCTACCCAG
    CCGACCACTT TGGCTTCCTC TGTAGACAGT GCACATTCAG ATGTTGCTTC AGGTATGAGT
    GATGGCAATG AGAACGTCCC ATCTTCCAGT GGAAGGCATG AAGGAAGAAC TACAAAACGG
    CATTACCGAA AATCTGTAAG GAGTCGCTCT CGACATGAAA AAACTTCACG CCCAAAATTA
    AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAATT AGAGACTCAT
    AATAGGAAAA TGGTTACATT CAAATTTGAC CTAGATGGTG ACAACCCCGA GGAGATAGCA
    ACAATTATGG TGAACAATGA CTTTATTCTA GCAATAGAGA GAGAGTCGTT TGTGGATCAA
    GTGCGAGAAA TTATTGAAAA AGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA
    GAGGGTGATC AGGGATTGGA GAGTCTACAA GGAAAGGATG ACTATGGCTT TTCAGGTTCT
    CAGAAATTGG AAGGAGAGTT CAAACAACCA ATTCCTGCGT CTTCCATGCC ACAGCAAATA
    GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CGGGAAGGCG GTTTATAGTG
    AGTCCTGTGC CAGAAAGCCG ATTACGAGAA TCAAAAGTTT TCCCCAGTGA AATAACAGAT
    ACAGTTGCTG CCTCTACAGC TCAGAGCCCT GGAATGAACT TGTCTCACTC TGCATCATCC
    CTTAGTCTAC AACAGGCCTT TTCTGAACTT AGACGTGCCC AAATGACAGA AGGACCCAAC
    ACAGCACCTC CAAACTTTAG TCATACAGGA CCAACATTTC CAGTAGTACC TCCTTTCTTA
    AGTAGCATTG CTGGAGTCCC AACCACAGCA GCAGCCACAG CACCAGTCCC TGCAACAAGC
    AGCCCTCCTA ATGACATTTC CACATCAGTA ATTCAGTCTG AGGTTACAGT GCCCACTGAA
    GAGGGGATTG CTGGAGTTGC CACCAGCACA GGTGTGGTAA CTTCAGGTGG TCTCCCCATA
    CCACCTGTGT CTGAATCACC AGTACTTTCC AGCGTAGTTT CAAGTATCAC AATACCTGCA
    GTTGTCTCAA TATCTACTAC ATCCCCGTCA CTTCAAGTCC CCACATCCAC ATCTGAGATC
    GTTGTTTCTA GTACAGCACT GTATCCTTCA GTAACAGTTT CAGCAACTTC AGCCTCTGCA
    GGGGGCAGTA CTGCTACCCC AGGTCCTAAG CCTCCAGCTG TAGTATCTCA GCAGGCAGCA
    GGCAGCACTA CTGTGGGAGC CACATTAACA TCAGTTTCTA CCACCACTTC ATTCCCAAGC
    ACAGCTTCAC AGCTGTGCAT TCAGCTTAGC AGCAGTACTT CTACTCCTAC TTTAGCTGAA
    ACCGTGGTAG TTAGCGCACA CTCACTAGAT AAGACATCTC ATAGCAGTAC AACTGGATTG
    GCTTTCTCCC TCTCTGCACC ATCTTCCTCT TCCTCTCCTG GAGCAGGAGT GTCTAGTTAT
    ATTTCTCAGC CTGGTGGGCT GCATCCTTTG GTCATTCCAT CAGTGATAGC TTCTACTCCT
    ATTCTTCCCC AAGCAGCAGG ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAGTATC
    CCACCCTTGG TACAGCCTGT TGCCAATGTG CCTGCTGTAC AGCAGACACT AATTCATAGT
    CAGCCTCAAC CAGCTTTGCT TCCCAACCAG CCCCATACTC ATTGTCCTGA AGTAGATTCT
    GATACACAAC CCAAAGCTCC TGGAATTGAT GACATAAAGA CTCTAGAAGA AAAGCTGCGG
    TCTCTGTTCA GTGAACACAG CTCATCTGGA GCTCAGCATG CCTCTGTCTC ACTGGAGACC
    TCACTAGTCA TAGAGAGCAC TGTCACACCA GGCATCCCAA CTACTGCTGT TGCACCAAGC
    AAACTCCTGA CTTCTACCAC AAGTACTTGC TTACCACCAA CCAATTTACC ACTAGGAACA
    GTTGCTTTGC CAGTTACACC AGTGGTCACA CCTGGGCAAG TTTCTACCCC AGTCAGCACT
    ACTACATCAG GAGTGAAACC TGGAACTGCT CCCTCCAAGC CACCTCTAAC TAAGGCTCCG
    GTGCTGCCAG TGGGTACTGA ACTTCCAGCA GGTACTCTAC CCAGCGAGCA GCTGCCACCT
    TTTCCAGGAC CTTCTCTAAC CCAGTCCCAG CAACCTCTAG AGGATCTTGA TGCTCAATTG
    AGAAGAACAC TTAGTCCAGA GATGATCACA GTGACTTCTG CGGTTGGTCC TGTGTCCATG
    GCGGCTCCAA CAGCAATCAC AGAAGCAGGA ACACAGCCTC AGAAGGGTGT TTCTCAAGTC
    AAAGAAGGCC CTGTCCTAGC AACTAGTTCA GGAGCTGGTG TTTTTAAGAT GGGACGATTT
    CAGGTTTCTG TTGCAGCAGA CGGTGCCCAG AAAGAGGGTA AAAATAAGTC AGAAGATGCA
    AAGTCTGTTC ATTTTGAATC CAGCACCTCA GAGTCCTCAG TGCTATCAAG TAGTAGTCCA
    GAGAGTACCT TGGTGAAACC AGAGCCGAAT GGCATAACCA TCCCTGGTAT CTCTTCAGAT
    GTGCCAGAGA GTGCCCACAA AACTACTGCC TCAGAGGCAA AGTCAGACAC TGGGCAGCCT
    ACCAAGGTTG GACGTTTTCA GGTGACAACT ACAGCAAACA AAGTGGGTCG TTTCTCTGTA
    TCAAAAACTG AGGACAAGAT CACTGACACA AAGAAAGAAG GACCAGTGGC ATCTCCTCCT
    TTTATGGATT TGGAACAAGC TGTTCTTCCT GCTGTGATAC CAAAGAAAGA GAAGCCTGAA
    CTGTCAGAGC CTTCACATCT AAATGGGCCG TCTTCTGACC CGGAGGCCGC TTTTTTAAGT
    AGGGATGTGG ATGATGGTTC CGGTAGTCCA CACTCGCCCC ATCAGCTGAG CTCAAAGAGC
    CTTCCTAGCC AGAATCTAAG TCAAAGCCTT AGTAATTCAT TTAACTCCTC TTACATGAGT
    AGCGACAATG AGTCAGATAT CGAAGATGAA GACTTAAAGT TAGAGCTGCG ACGACTACGA
    GATAAACATC TCAAAGAGAT TCAGGACCTG CAGAGTCGCC AGAAGCATGA AATTGAATCT
    TTGTATACCA AACTGGGCAA GGTGCCCCCT GCTGTTATTA TTCCCCCAGC TGCTCCCCTT
    TCAGGGAGAA GACGACGACC CACTAAAAGC AAAGGCAGCA AATCTAGTCG AAGCAGTTCC
    TTGGGGAATA AAAGCCCCCA GCTTTCAGGT AACCTGTCTG GTCAGAGTGC AGCTTCAGTC
    TTGCACCCCC AGCAGACCCT CCACCCTCCT GGCAACATCC CAGAGTCCGG GCAGAATCAG
    CTGTTACAGC CCCTTAAGCC ATCTCCCTCC AGTGACAACC TCTATTCAGC CTTCACCAGT
    GATGGTGCCA TTTCAGTACC AAGCCTTTCT GCTCCAGGTC AAGGAACCAG CAGCACAAAC
    ACTGTTGGGG CAACAGTGAA CAGCCAAGCC GCCCAAGCTC AGCCTCCTGC CATGACGTCC
    AGCAGGAAGG GCACATTCAC AGATGACTTG CACAAGTTGG TAGACAATTG GGCCCGAGAT
    GCCATGAATC TCTCAGGCAG GAGAGGAAGC AAAGGGCACA TGAATTACGA GGGCCCTGGA
    ATGGCAAGGA AGTTCTCTGC ACCTGGGCAA CTGTGCATCT CCATGACCTC GAACCTGGGT
    GGCTCTGCCC CCATCTCTGC AGCATCAGCT ACCTCTCTAG GTCACTTCAC CAAGTCTATG
    TGCCCCCCAC AGCAGTATGG CTTTCCAGCT ACCCCATTTG GCGCTCAATG GAGTGGGACG
    GGTGGCCCAG CACCACAGCC ACTTGGCCAG TTCCAACCTG TGGGAACTGC CTCCTTGCAG
    AATTTCAACA TCAGCAATTT GCAGAAATCC ATCAGCAACC CCCCAGGCTC CAACCTGCGG
    ACCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011519303.1
    CDS969..9077
    Translation

    Target ORF information:

    RefSeq Version XM_011521001.2
    Organism Homo sapiens(human)
    Definition Homo sapiens WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011521001.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG 
    CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA
    GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT
    ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC
    CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT
    TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC
    CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT
    GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC
    AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG
    GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG
    CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT
    CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC
    ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT
    GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT
    GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG
    ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG
    ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT
    CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT
    ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC
    AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT
    GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT
    CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG
    CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA
    CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA
    GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA
    AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA
    GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG
    GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC
    AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA
    AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG
    ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA
    GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA
    CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA
    TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA
    CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA
    CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGC CTCAGTCCAT GGCGCATCCG
    TGTGGGGGGA CCCCAACATA CCCAGAATCA CAGATATTTT TCCCAACTAT TCATGAACGT
    CCAGTTTCTT TTTCACCACC TCCCACCTGC CCACCGAAAG TAGCCATTTC CCAGCGGCGT
    AAGAGCACCT CCTTCCTGGA AGCCCAAACT CACCACTTCC AACCCCTGCT GAGGACTGTT
    GGCCAAAGTC TTCTTCCACC TGGTGGCAGC CCAACTAACT GGACACCAGA GGCCGTAGTT
    ATGTTGGGTA CTACAGCCAG TAGAGTAACT GGAGAGTCAT GTGAGATACA GGTCCATCCT
    ATGTTTGAAC CATCTCAAGT TTACAGTGAC TATAGACCTG GACTAGTACT TCCAGAAGAA
    GCTCACTATT TTATTCCTCA GGAAGCAGTG TATGTAGCTG GGGTACATTA CCAGGCCCGG
    GTGGCAGAAC AGTATGAGGG CATTCCATAC AACTCATCAG TACTGTCAAG TCCTATGAAA
    CAGATACCTG AACAGAAGCC AGTACAAGGG GGCCCTACTT CAAGTTCTGT CTTTGAATTT
    CCATCTGGAC AGGCTTTCCT GGTAGGACAC CTTCAGAATC TAAGATTAGA TTCTGGATTG
    GGTCCGGGAT CTCCCCTCTC TAGTATTTCT GCACCTATCA GTACAGATGC TACACGTTTG
    AAATTTCACC CTGTCTTTGT TCCTCATTCT GCGCCTGCTG TGTTAACTCA TAACAATGAG
    AGCAGAAGCA ACTGTGTATT TGAATTTCAT GTTCACACAC CAAGCTCCTC TTCAGGAGAA
    GGAGGTGGAA TTTTACCTCA GCGTGTTTAC CGAAATCGGC AGGTTGCAGT GGACTTGAAT
    CAAGAAGAAC TGCCTCCTCA ATCAGTTGGA TTACATGGCT ACTTGCAGCC TGTGACTGAA
    GAAAAGCATA ATTACCATGC CCCAGAATTG ACCGTTTCTG TGGTAGAGCC TATCGGACAG
    AACTGGCCAA TAGGAAGCCC AGAATATTCC AGTGATTCCT CACAAATCAC TTCTTCAGAC
    CCCAGTGATT TTCAGTCACC TCCCCCTACA GGGGGAGCAG CTGCACCTTT TGGCTCTGAC
    GTCTCAATGC CCTTTATCCA TCTGCCTCAG ACAGTGTTAC AAGAATCCCC ACTTTTCTTC
    TGTTTCCCCC AAGGAACCAC ATCTCAGCAG GTCTTAACTG CCTCATTTTC TTCAGGAGGA
    TCTGCACTTC ATCCACAGGC ACAGGGGCAG AGCCAGGGTC AGCCATCCTC AAGTAGCTTA
    ACAGGGGTTT CATCTTCCCA ACCCATACAA CATCCTCAGC AGCAGCAGGG AATACAGCAG
    ACAGCCCCTC CTCAACAGAC AGTGCAGTAT TCACTTTCAC AGACATCAAC CTCCAGTGAG
    GCCACTACTG CACAGCCAGT GAGTCAGCCT CAAGCTCCAC AAGTCTTGCC TCAAGTATCA
    GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA GTACCAACTA TCCAAGGCGA ACCTCAGATC
    CCAGTTGCGA CACAACCCTC GGTTGTTCCA GTCCACTCTG GTGCTCATTT CCTTCCAGTG
    GGACAGCCGC TCCCTACTCC CTTGCTCCCT CAGTACCCTG TCTCTCAGAT TCCCATATCA
    ACTCCTCATG TGTCTACGGC TCAGACAGGT TTCTCATCCC TTCCCATCAC AATGGCAGCT
    GGCATTACTC AGCCTCTGCT CACGTTGGCT TCATCTGCTA CAACAGCTGC GATCCCGGGG
    GTATCAACTG TGGTTCCTAG TCAGCTTCCA ACCCTTCTGC AGCCTGTGAC TCAGCTGCCA
    AGTCAGGTTC ACCCACAGCT CCTACAACCA GCAGTTCAGT CCATGGGAAT ACCAGCTAAC
    CTTGGACAAG CTGCTGAGGT TCCACTTTCC TCTGGAGATG TTCTGTACCA GAGTACTCAG
    GGAGTCTCTC AGGTTGCTCC TGCAGAGCCA GTTGCAGTAG CACAGACCCA AGCTACCCAG
    CCGACCACTT TGGCTTCCTC TGTAGACAGT GCACATTCAG ATGTTGCTTC AGGTATGAGT
    GATGGCAATG AGAACGTCCC ATCTTCCAGT GGAAGGCATG AAGGAAGAAC TACAAAACGG
    CATTACCGAA AATCTGTAAG GAGTCGCTCT CGACATGAAA AAACTTCACG CCCAAAATTA
    AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAATT AGAGACTCAT
    AATAGGAAAA TGGTTACATT CAAATTTGAC CTAGATGGTG ACAACCCCGA GGAGATAGCA
    ACAATTATGG TGAACAATGA CTTTATTCTA GCAATAGAGA GAGAGTCGTT TGTGGATCAA
    GTGCGAGAAA TTATTGAAAA AGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA
    GAGGGTGATC AGGGATTGGA GAGTCTACAA GGAAAGGATG ACTATGGCTT TTCAGGTTCT
    CAGAAATTGG AAGGAGAGTT CAAACAACCA ATTCCTGCGT CTTCCATGCC ACAGCAAATA
    GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CGGGAAGGCG GTTTATAGTG
    AGTCCTGTGC CAGAAAGCCG ATTACGAGAA TCAAAAGTTT TCCCCAGTGA AATAACAGAT
    ACAGTTGCTG CCTCTACAGC TCAGAGCCCT GGAATGAACT TGTCTCACTC TGCATCATCC
    CTTAGTCTAC AACAGGCCTT TTCTGAACTT AGACGTGCCC AAATGACAGA AGGACCCAAC
    ACAGCACCTC CAAACTTTAG TCATACAGGA CCAACATTTC CAGTAGTACC TCCTTTCTTA
    AGTAGCATTG CTGGAGTCCC AACCACAGCA GCAGCCACAG CACCAGTCCC TGCAACAAGC
    AGCCCTCCTA ATGACATTTC CACATCAGTA ATTCAGTCTG AGGTTACAGT GCCCACTGAA
    GAGGGGATTG CTGGAGTTGC CACCAGCACA GGTGTGGTAA CTTCAGGTGG TCTCCCCATA
    CCACCTGTGT CTGAATCACC AGTACTTTCC AGCGTAGTTT CAAGTATCAC AATACCTGCA
    GTTGTCTCAA TATCTACTAC ATCCCCGTCA CTTCAAGTCC CCACATCCAC ATCTGAGATC
    GTTGTTTCTA GTACAGCACT GTATCCTTCA GTAACAGTTT CAGCAACTTC AGCCTCTGCA
    GGGGGCAGTA CTGCTACCCC AGGTCCTAAG CCTCCAGCTG TAGTATCTCA GCAGGCAGCA
    GGCAGCACTA CTGTGGGAGC CACATTAACA TCAGTTTCTA CCACCACTTC ATTCCCAAGC
    ACAGCTTCAC AGCTGTGCAT TCAGCTTAGC AGCAGTACTT CTACTCCTAC TTTAGCTGAA
    ACCGTGGTAG TTAGCGCACA CTCACTAGAT AAGACATCTC ATAGCAGTAC AACTGGATTG
    GCTTTCTCCC TCTCTGCACC ATCTTCCTCT TCCTCTCCTG GAGCAGGAGT GTCTAGTTAT
    ATTTCTCAGC CTGGTGGGCT GCATCCTTTG GTCATTCCAT CAGTGATAGC TTCTACTCCT
    ATTCTTCCCC AAGCAGCAGG ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAGTATC
    CCACCCTTGG TACAGCCTGT TGCCAATGTG CCTGCTGTAC AGCAGACACT AATTCATAGT
    CAGCCTCAAC CAGCTTTGCT TCCCAACCAG CCCCATACTC ATTGTCCTGA AGTAGATTCT
    GATACACAAC CCAAAGCTCC TGGAATTGAT GACATAAAGA CTCTAGAAGA AAAGCTGCGG
    TCTCTGTTCA GTGAACACAG CTCATCTGGA GCTCAGCATG CCTCTGTCTC ACTGGAGACC
    TCACTAGTCA TAGAGAGCAC TGTCACACCA GGCATCCCAA CTACTGCTGT TGCACCAAGC
    AAACTCCTGA CTTCTACCAC AAGTACTTGC TTACCACCAA CCAATTTACC ACTAGGAACA
    GTTGCTTTGC CAGTTACACC AGTGGTCACA CCTGGGCAAG TTTCTACCCC AGTCAGCACT
    ACTACATCAG GAGTGAAACC TGGAACTGCT CCCTCCAAGC CACCTCTAAC TAAGGCTCCG
    GTGCTGCCAG TGGGTACTGA ACTTCCAGCA GGTACTCTAC CCAGCGAGCA GCTGCCACCT
    TTTCCAGGAC CTTCTCTAAC CCAGTCCCAG CAACCTCTAG AGGATCTTGA TGCTCAATTG
    AGAAGAACAC TTAGTCCAGA GATGATCACA GTGACTTCTG CGGTTGGTCC TGTGTCCATG
    GCGGCTCCAA CAGCAATCAC AGAAGCAGGA ACACAGCCTC AGAAGGGTGT TTCTCAAGTC
    AAAGAAGGCC CTGTCCTAGC AACTAGTTCA GGAGCTGGTG TTTTTAAGAT GGGACGATTT
    CAGGTTTCTG TTGCAGCAGA CGGTGCCCAG AAAGAGGGTA AAAATAAGTC AGAAGATGCA
    AAGTCTGTTC ATTTTGAATC CAGCACCTCA GAGTCCTCAG TGCTATCAAG TAGTAGTCCA
    GAGAGTACCT TGGTGAAACC AGAGCCGAAT GGCATAACCA TCCCTGGTAT CTCTTCAGAT
    GTGCCAGAGA GTGCCCACAA AACTACTGCC TCAGAGGCAA AGTCAGACAC TGGGCAGCCT
    ACCAAGGTTG GACGTTTTCA GGTGACAACT ACAGCAAACA AAGTGGGTCG TTTCTCTGTA
    TCAAAAACTG AGGACAAGAT CACTGACACA AAGAAAGAAG GACCAGTGGC ATCTCCTCCT
    TTTATGGATT TGGAACAAGC TGTTCTTCCT GCTGTGATAC CAAAGAAAGA GAAGCCTGAA
    CTGTCAGAGC CTTCACATCT AAATGGGCCG TCTTCTGACC CGGAGGCCGC TTTTTTAAGT
    AGGGATGTGG ATGATGGTTC CGGTAGTCCA CACTCGCCCC ATCAGCTGAG CTCAAAGAGC
    CTTCCTAGCC AGAATCTAAG TCAAAGCCTT AGTAATTCAT TTAACTCCTC TTACATGAGT
    AGCGACAATG AGTCAGATAT CGAAGATGAA GACTTAAAGT TAGAGCTGCG ACGACTACGA
    GATAAACATC TCAAAGAGAT TCAGGACCTG CAGAGTCGCC AGAAGCATGA AATTGAATCT
    TTGTATACCA AACTGGGCAA GGTGCCCCCT GCTGTTATTA TTCCCCCAGC TGCTCCCCTT
    TCAGGGAGAA GACGACGACC CACTAAAAGC AAAGGCAGCA AATCTAGTCG AAGCAGTTCC
    TTGGGGAATA AAAGCCCCCA GCTTTCAGGT AACCTGTCTG GTCAGAGTGC AGCTTCAGTC
    TTGCACCCCC AGCAGACCCT CCACCCTCCT GGCAACATCC CAGAGTCCGG GCAGAATCAG
    CTGTTACAGC CCCTTAAGCC ATCTCCCTCC AGTGACAACC TCTATTCAGC CTTCACCAGT
    GATGGTGCCA TTTCAGTACC AAGCCTTTCT GCTCCAGGTC AAGGAACCAG CAGCACAAAC
    ACTGTTGGGG CAACAGTGAA CAGCCAAGCC GCCCAAGCTC AGCCTCCTGC CATGACGTCC
    AGCAGGAAGG GCACATTCAC AGATGACTTG CACAAGTTGG TAGACAATTG GGCCCGAGAT
    GCCATGAATC TCTCAGGCAG GAGAGGAAGC AAAGGGCACA TGAATTACGA GGGCCCTGGA
    ATGGCAAGGA AGTTCTCTGC ACCTGGGCAA CTGTGCATCT CCATGACCTC GAACCTGGGT
    GGCTCTGCCC CCATCTCTGC AGCATCAGCT ACCTCTCTAG GTCACTTCAC CAAGTCTATG
    TGCCCCCCAC AGCAGTATGG CTTTCCAGCT ACCCCATTTG GCGCTCAATG GAGTGGGACG
    GGTGGCCCAG CACCACAGCC ACTTGGCCAG TTCCAACCTG TGGGAACTGC CTCCTTGCAG
    AATTTCAACA TCAGCAATTT GCAGAAATCC ATCAGCAACC CCCCAGGCTC CAACCTGCGG
    ACCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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