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WNK4 cDNA ORF clone, Homo sapiens (human)

Gene Symbol WNK4
Entrez Gene ID 65266
Full Name WNK lysine deficient protein kinase 4
Synonyms PHA2B, PRKWNK4
General protein information
Preferred Names
serine/threonine-protein kinase WNK4
Names
serine/threonine-protein kinase WNK4
protein kinase with no lysine 4
protein kinase lysine-deficient 4
NP_115763.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

17

17q21.2

Summary This gene encodes a member of the WNK family of serine-threonine protein kinases. The kinase is part of the tight junction complex in kidney cells, and regulates the balance between NaCl reabsorption and K(+) secretion. The kinase regulates the activities of several types of ion channels, cotransporters, and exchangers involved in electrolyte flux in epithelial cells. Mutations in this gene result in pseudohypoaldosteronism type IIB.[provided by RefSeq, Sep 2009]. lac of sum
Disorder MIM:

601844

Disorder Html: Pseudohypoaldosteronism type II, 145260 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_032387 NP_115763 serine/threonine-protein kinase WNK4
XM_005257595 XP_005257652 serine/threonine-protein kinase WNK4 isoform X1
XM_011525132 XP_011523434 serine/threonine-protein kinase WNK4 isoform X2
XM_011525133 XP_011523435 serine/threonine-protein kinase WNK4 isoform X3
XM_005257596 XP_005257653 serine/threonine-protein kinase WNK4 isoform X4
XM_005257597 XP_005257654 serine/threonine-protein kinase WNK4 isoform X5
XM_006722020 XP_006722083 serine/threonine-protein kinase WNK4 isoform X6
XM_011525134 XP_011523436 serine/threonine-protein kinase WNK4 isoform X7
XM_011525135 XP_011523437 serine/threonine-protein kinase WNK4 isoform X8
XM_006722021 XP_006722084 serine/threonine-protein kinase WNK4 isoform X9
XM_006722022 XP_006722085 serine/threonine-protein kinase WNK4 isoform X10



Homo sapiens (human) WNK4 NP_115763.2
Pan troglodytes (chimpanzee) WNK4 XP_003315535.1
Macaca mulatta (Rhesus monkey) LOC707820 XP_002808177.1
Canis lupus familiaris (dog) WNK4 XP_548082.3
Bos taurus (cattle) WNK4 XP_003587489.1
Mus musculus (house mouse) Wnk4 NP_783569.1
Rattus norvegicus (Norway rat) Wnk4 NP_783169.2
Gallus gallus (chicken) LOC777580 XP_003642875.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following WNK4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WNK4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu17844 NM_032387 Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $384.30
$549.00
OHu50593 XM_005257595 PREDICTED: Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $643.30
$919.00
OHu71641 XM_011525132 PREDICTED: Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $643.30
$919.00
OHu71642 XM_011525133 PREDICTED: Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $643.30
$919.00
OHu50594 XM_005257596 PREDICTED: Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $643.30
$919.00
OHu50595 XM_005257597 PREDICTED: Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $643.30
$919.00
OHu50596 XM_006722020 PREDICTED: Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $643.30
$919.00
OHu71643 XM_011525134 PREDICTED: Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $643.30
$919.00
OHu71644 XM_011525135 PREDICTED: Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $643.30
$919.00
OHu50598 XM_006722021 PREDICTED: Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu50599 XM_006722022 PREDICTED: Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant X10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu17844
Clone ID Related Accession (Same CDS sequence) NM_032387
Accession Version NM_032387.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3732bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-JUL-2015
Organism Homo sapiens (human)
Product serine/threonine-protein kinase WNK4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CA388932.1, BC136664.1 and BM683764.1. This sequence is a reference standard in the RefSeqGene project. On Oct 1, 2009 this sequence version replaced gi:58331254. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC136664.1, AJ316534.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
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661
721
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841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGTTGGCAT CCCCGGCCAC GGAGACCACC GTCCTCATGT CCCAGACTGA GGCCGACCTG 
GCCCTGCGGC CCCCGCCTCC TCTTGGCACC GCGGGGCAGC CCCGCCTCGG GCCCCCTCCT
CGCCGAGCGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC TCGTCTCAGC
CGCCGTAGCT CAGTCGACTT GGGGCTGCTG AGCTCTTGGT CCCTGCCAGC CTCACCCGCT
CCGGACCCCC CCGATCCTCC GGACTCCGCT GGTCCTGGCC CCGCGAGGAG CCCACCGCCT
AGCTCCAAAG AACCCCCCGA GGGCACGTGG ACCGAGGGAG CCCCTGTGAA GGCTGCGGAA
GACTCCGCGC GTCCCGAGCT CCCGGACTCT GCAGTGGGCC CGGGGTCCAG GGAGCCGCTA
AGGGTCCCTG AAGCTGTGGC CCTAGAGCGG CGGCGGGAGC AGGAAGAAAA GGAGGACATG
GAGACCCAGG CTGTGGCAAC GTCCCCCGAT GGCCGATACC TCAAGTTTGA CATCGAGATT
GGACGTGGCT CCTTCAAGAC GGTGTATCGA GGGCTAGACA CCGACACCAC AGTGGAGGTG
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTAGAGCTG AGCGGCAGCG CTTCTCAGAG
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA TGATTCGTGG
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
ACGCTCAAGA CGTACCTGAG GCGGTTCCGG GAGATGAAGC CGCGGGTCCT TCAGCGCTGG
AGCCGCCAAA TCCTGCGGGG ACTTCATTTC CTACACTCCC GGGTTCCTCC CATCCTGCAC
CGGGATCTCA AGTGCGACAA TGTCTTTATC ACGGGACCTA CTGGCTCTGT CAAAATCGGG
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTTGCCA AGAGTGTCAT CGGGACCCCG
GAATTCATGG CCCCCGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
TTCGGCATGT GCATGCTGGA GATGGCCACC TCTGAGTACC CGTACTCCGA GTGCCAGAAT
GCCGCGCAAA TCTACCGCAA GGTCACTTCG GGCAGAAAGC CGAACAGCTT CCACAAGGTG
AAGATACCCG AGGTGAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAACGAGAGG
TTCACCATCC AGGACCTCCT GGCCCACGCC TTCTTCCGCG AGGAGCGCGG TGTGCACGTG
GAACTAGCGG AGGAGGACGA CGGCGAGAAG CCGGGCCTCA AGCTCTGGCT GCGCATGGAG
GACGCGCGGC GCGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCCT GTTCCAGCTG
GGCCGGGACG CGGCCGAGGA GGTGGCACAG GAGATGGTGG CTCTGGGCTT GGTCTGTGAA
GCCGATTACC AGCCAGTGGC CCGTGCAGTA CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
CGTGAGAAGC TGCGTAAAGC AAGGGAATTG GAGGCACTCC CACCAGAGCC AGGACCTCCA
CCAGCAACTG TGCCCATGGC CCCCGGTCCC CCCAGTGTCT TCCCCCCTGA GCCTGAGGAG
CCAGAGGCAG ACCAGCACCA GCCCTTCCTT TTCCGCCACG CCAGCTACTC ATCTACCACT
TCGGATTGCG AGACTGATGG CTACCTCAGC TCCTCCGGCT TCCTGGATGC CTCAGACCCT
GCCCTTCAGC CCCCTGGGGG GGTGCCATCC AGCCTGGCTG AGTCCCATCT CTGCCTGCCC
TCGGCTTTTG CCCTATCCAT TCCACGTTCT GGCCCTGGAA GTGACTTTTC CCCCGGGGAC
AGCTATGCCT CAGATGCAGC TTCAGGCCTT AGCGATGTGG GAGAAGGGAT GGGACAAATG
AGGAGACCCC CAGGGAGGAA TCTCCGGCGC AGACCCCGAT CCCGGCTGCG GGTCACTAGT
GTCTCAGACC AGAATGACAG AGTGGTTGAG TGCCAGCTAC AGACCCATAA CAGCAAGATG
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCGGAAG AGATTGCAGC TGCCATGGTA
TATAACGAGT TCATTCTGCC TTCGGAGCGA GATGGATTTC TCAGACGGAT TCGGGAGATT
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GACACTGGCC CCATGGAGGC TGCTGAAGAC
ACCCTAAGCC CCCAGGAGGA GCCAGCACCA TTACCTGCCC TGCCCGTCCC CCTCCCAGAC
CCATCCAATG AAGAGCTCCA GAGCAGCACC TCCCTGGAGC ACAGGAGCTG GACAGCCTTC
TCCACCTCCT CATCTTCTCC TGGAACTCCT TTGTCTCCTG GAAACCCATT TTCCCCTGGA
ACCCCCATTT CCCCAGGTCC CATCTTCCCC ATCACTTCTC CCCCATGTCA TCCCAGCCCC
TCCCCATTCT CCCCCATTTC TTCCCAGGTC TCCTCAAATC CCTCTCCACA CCCCACCAGC
TCTCCACTTC CATTCTCCTC CAGCACACCC GAGTTTCCGG TCCCACTCTC TCAGTGTCCC
TGGAGTTCTC TCCCCACGAC TTCTCCACCT ACGTTCTCTC CCACTTGTTC TCAGGTCACT
CTTAGTTCCC CTTTCTTTCC TCCGTGCCCC TCCACTTCTT CCTTCCCCTC CACCACAGCA
GCCCCTCTCC TTTCTCTGGC TAGTGCCTTC TCACTGGCTG TGATGACTGT GGCCCAGTCC
CTGCTGTCCC CCTCACCTGG GCTCCTTTCC CAGTCTCCTC CAGCCCCTCC TAGTCCCCTC
CCTAGCCTGC CCCTTCCCCC TCCCGTTGCT CCTGGTGGCC AGGAAAGCCC TTCACCCCAC
ACAGCTGAGG TGGAGAGTGA GGCCTCACCA CCTCCTGCTC GGCCCCTCCC AGGGGAAGCC
AGGCTGGCGC CCATCTCTGA AGAGGGAAAG CCGCAGCTTG TTGGGCGTTT CCAAGTGACT
TCATCCAAGG AACCGGCTGA GCCTCTTCCC TTGCAGCCAA CATCCCCCAC TCTCTCTGGT
TCTCCAAAAC CTTCAACCCC TCAGCTCACT TCAGAGAGCT CAGATACAGA GGACAGTGCT
GGAGGCGGGC CAGAGACCAG GGAAGCTCTG GCTGAGAGCG ACCGTGCAGC TGAGGGTCTG
GGGGCTGGAG TTGAGGAGGA AGGAGATGAT GGGAAGGAAC CCCAAGTTGG GGGCAGCCCC
CAACCCCTGA GCCATCCCAG CCCAGTGTGG ATGAACTACT CCTACAGCAG CCTGTGTTTG
AGCAGCGAGG AGTCAGAAAG CAGTGGGGAA GATGAGGAGT TCTGGGCTGA GCTGCAGAGT
CTTCGGCAGA AGCACTTGTC AGAGGTGGAA ACACTACAGA CACTACAGAA AAAAGAAATT
GAAGATTTGT ACAGCCGGCT GGGGAAGCAG CCCCCACCGG GTATTGTGGC CCCAGCTGCT
ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC AAGGGCAGCT TCCCCACCTC CCGCCGCAAC
AGCCTACAGC GCTCTGAGCC CCCAGGCCCT GGCATCATGC GAAGGAACTC TCTGAGTGGC
AGCAGCACCG GCTCCCAGGA GCAGCGGGCA AGCAAGGGGG TGACATTCGC CGGGGATGTT
GGCAGGATGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_115763.2
CDS69..3800
Misc Feature(1)582..1364(+)
Misc Feature(2)603..1364(+)
Misc Feature(3)606..1091(+)
Misc Feature(4)606..1031(+)
Misc Feature(5)618..1091(+)
Misc Feature(6)1026..1091(+)
Misc Feature(7)1059..1061(+)
Misc Feature(8)1071..1073(+)
Misc Feature(9)1425..1535(+)
Exon (1)1..686
Gene:WNK4
Gene Synonym:
Exon (2)687..859
Gene:WNK4
Gene Synonym:
Exon (3)860..1080
Gene:WNK4
Gene Synonym:
Exon (4)1081..1238
Gene:WNK4
Gene Synonym:
Exon (5)1239..1327
Gene:WNK4
Gene Synonym:
Exon (6)1328..1544
Gene:WNK4
Gene Synonym:
Exon (7)1545..1809
Gene:WNK4
Gene Synonym:
Exon (8)1810..1931
Gene:WNK4
Gene Synonym:
Exon (9)1932..1990
Gene:WNK4
Gene Synonym:
Exon (10)1991..2108
Gene:WNK4
Gene Synonym:
Exon (11)2109..2225
Gene:WNK4
Gene Synonym:
Exon (12)2226..2363
Gene:WNK4
Gene Synonym:
Exon (13)2364..2418
Gene:WNK4
Gene Synonym:
Exon (14)2419..3029
Gene:WNK4
Gene Synonym:
Exon (15)3030..3090
Gene:WNK4
Gene Synonym:
Exon (16)3091..3499
Gene:WNK4
Gene Synonym:
Exon (17)3500..3699
Gene:WNK4
Gene Synonym:
Exon (18)3700..3797
Gene:WNK4
Gene Synonym:
Exon (19)3798..4178
Gene:WNK4
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_032387
Organism Homo sapiens (human)
Definition Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_032387

ORF Insert Sequence:

1
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121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGTTGGCAT CCCCGGCCAC GGAGACCACC GTCCTCATGT CCCAGACTGA GGCCGACCTG 
GCCCTGCGGC CCCCGCCTCC TCTTGGCACC GCGGGGCAGC CCCGCCTCGG GCCCCCTCCT
CGCCGAGCGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC TCGTCTCAGC
CGCCGTAGCT CAGTCGACTT GGGGCTGCTG AGCTCTTGGT CCCTGCCAGC CTCACCCGCT
CCGGACCCCC CCGATCCTCC GGACTCCGCT GGTCCTGGCC CCGCGAGGAG CCCACCGCCT
AGCTCCAAAG AACCCCCCGA GGGCACGTGG ACCGAGGGAG CCCCTGTGAA GGCTGCGGAA
GACTCCGCGC GTCCCGAGCT CCCGGACTCT GCAGTGGGCC CGGGGTCCAG GGAGCCGCTA
AGGGTCCCTG AAGCTGTGGC CCTAGAGCGG CGGCGGGAGC AGGAAGAAAA GGAGGACATG
GAGACCCAGG CTGTGGCAAC GTCCCCCGAT GGCCGATACC TCAAGTTTGA CATCGAGATT
GGACGTGGCT CCTTCAAGAC GGTGTATCGA GGGCTAGACA CCGACACCAC AGTGGAGGTG
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTAGAGCTG AGCGGCAGCG CTTCTCAGAG
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA TGATTCGTGG
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
ACGCTCAAGA CGTACCTGAG GCGGTTCCGG GAGATGAAGC CGCGGGTCCT TCAGCGCTGG
AGCCGCCAAA TCCTGCGGGG ACTTCATTTC CTACACTCCC GGGTTCCTCC CATCCTGCAC
CGGGATCTCA AGTGCGACAA TGTCTTTATC ACGGGACCTA CTGGCTCTGT CAAAATCGGG
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTTGCCA AGAGTGTCAT CGGGACCCCG
GAATTCATGG CCCCCGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
TTCGGCATGT GCATGCTGGA GATGGCCACC TCTGAGTACC CGTACTCCGA GTGCCAGAAT
GCCGCGCAAA TCTACCGCAA GGTCACTTCG GGCAGAAAGC CGAACAGCTT CCACAAGGTG
AAGATACCCG AGGTGAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAACGAGAGG
TTCACCATCC AGGACCTCCT GGCCCACGCC TTCTTCCGCG AGGAGCGCGG TGTGCACGTG
GAACTAGCGG AGGAGGACGA CGGCGAGAAG CCGGGCCTCA AGCTCTGGCT GCGCATGGAG
GACGCGCGGC GCGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCCT GTTCCAGCTG
GGCCGGGACG CGGCCGAGGA GGTGGCACAG GAGATGGTGG CTCTGGGCTT GGTCTGTGAA
GCCGATTACC AGCCAGTGGC CCGTGCAGTA CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
CGTGAGAAGC TGCGTAAAGC AAGGGAATTG GAGGCACTCC CACCAGAGCC AGGACCTCCA
CCAGCAACTG TGCCCATGGC CCCCGGTCCC CCCAGTGTCT TCCCCCCTGA GCCTGAGGAG
CCAGAGGCAG ACCAGCACCA GCCCTTCCTT TTCCGCCACG CCAGCTACTC ATCTACCACT
TCGGATTGCG AGACTGATGG CTACCTCAGC TCCTCCGGCT TCCTGGATGC CTCAGACCCT
GCCCTTCAGC CCCCTGGGGG GGTGCCATCC AGCCTGGCTG AGTCCCATCT CTGCCTGCCC
TCGGCTTTTG CCCTATCCAT TCCACGTTCT GGCCCTGGAA GTGACTTTTC CCCCGGGGAC
AGCTATGCCT CAGATGCAGC TTCAGGCCTT AGCGATGTGG GAGAAGGGAT GGGACAAATG
AGGAGACCCC CAGGGAGGAA TCTCCGGCGC AGACCCCGAT CCCGGCTGCG GGTCACTAGT
GTCTCAGACC AGAATGACAG AGTGGTTGAG TGCCAGCTAC AGACCCATAA CAGCAAGATG
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCGGAAG AGATTGCAGC TGCCATGGTA
TATAACGAGT TCATTCTGCC TTCGGAGCGA GATGGATTTC TCAGACGGAT TCGGGAGATT
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GACACTGGCC CCATGGAGGC TGCTGAAGAC
ACCCTAAGCC CCCAGGAGGA GCCAGCACCA TTACCTGCCC TGCCCGTCCC CCTCCCAGAC
CCATCCAATG AAGAGCTCCA GAGCAGCACC TCCCTGGAGC ACAGGAGCTG GACAGCCTTC
TCCACCTCCT CATCTTCTCC TGGAACTCCT TTGTCTCCTG GAAACCCATT TTCCCCTGGA
ACCCCCATTT CCCCAGGTCC CATCTTCCCC ATCACTTCTC CCCCATGTCA TCCCAGCCCC
TCCCCATTCT CCCCCATTTC TTCCCAGGTC TCCTCAAATC CCTCTCCACA CCCCACCAGC
TCTCCACTTC CATTCTCCTC CAGCACACCC GAGTTTCCGG TCCCACTCTC TCAGTGTCCC
TGGAGTTCTC TCCCCACGAC TTCTCCACCT ACGTTCTCTC CCACTTGTTC TCAGGTCACT
CTTAGTTCCC CTTTCTTTCC TCCGTGCCCC TCCACTTCTT CCTTCCCCTC CACCACAGCA
GCCCCTCTCC TTTCTCTGGC TAGTGCCTTC TCACTGGCTG TGATGACTGT GGCCCAGTCC
CTGCTGTCCC CCTCACCTGG GCTCCTTTCC CAGTCTCCTC CAGCCCCTCC TAGTCCCCTC
CCTAGCCTGC CCCTTCCCCC TCCCGTTGCT CCTGGTGGCC AGGAAAGCCC TTCACCCCAC
ACAGCTGAGG TGGAGAGTGA GGCCTCACCA CCTCCTGCTC GGCCCCTCCC AGGGGAAGCC
AGGCTGGCGC CCATCTCTGA AGAGGGAAAG CCGCAGCTTG TTGGGCGTTT CCAAGTGACT
TCATCCAAGG AACCGGCTGA GCCTCTTCCC TTGCAGCCAA CATCCCCCAC TCTCTCTGGT
TCTCCAAAAC CTTCAACCCC TCAGCTCACT TCAGAGAGCT CAGATACAGA GGACAGTGCT
GGAGGCGGGC CAGAGACCAG GGAAGCTCTG GCTGAGAGCG ACCGTGCAGC TGAGGGTCTG
GGGGCTGGAG TTGAGGAGGA AGGAGATGAT GGGAAGGAAC CCCAAGTTGG GGGCAGCCCC
CAACCCCTGA GCCATCCCAG CCCAGTGTGG ATGAACTACT CCTACAGCAG CCTGTGTTTG
AGCAGCGAGG AGTCAGAAAG CAGTGGGGAA GATGAGGAGT TCTGGGCTGA GCTGCAGAGT
CTTCGGCAGA AGCACTTGTC AGAGGTGGAA ACACTACAGA CACTACAGAA AAAAGAAATT
GAAGATTTGT ACAGCCGGCT GGGGAAGCAG CCCCCACCGG GTATTGTGGC CCCAGCTGCT
ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC AAGGGCAGCT TCCCCACCTC CCGCCGCAAC
AGCCTACAGC GCTCTGAGCC CCCAGGCCCT GGCATCATGC GAAGGAACTC TCTGAGTGGC
AGCAGCACCG GCTCCCAGGA GCAGCGGGCA AGCAAGGGGG TGACATTCGC CGGGGATGTT
GGCAGGATGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50593
Clone ID Related Accession (Same CDS sequence) XM_005257595
Accession Version XM_005257595.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3840bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product serine/threonine-protein kinase WNK4 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578831263. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGTTGGCAT CCCCGGCCAC GGAGACCACC GTCCTCATGT CCCAGACTGA GGCCGACCTG 
GCCCTGCGGC CCCCGCCTCC TCTTGGCACC GCGGGGCAGC CCCGCCTCGG GCCCCCTCCT
CGCCGAGCGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC TCGTCTCAGC
CGCCGTAGCT CAGTCGACTT GGGGCTGCTG AGCTCTTGGT CCCTGCCAGC CTCACCCGCT
CCGGACCCCC CCGATCCTCC GGACTCCGCT GGTCCTGGCC CCGCGAGGAG CCCACCGCCT
AGCTCCAAAG AACCCCCCGA GGGCACGTGG ACCGAGGGAG CCCCTGTGAA GGCTGCGGAA
GACTCCGCGC GTCCCGAGCT CCCGGACTCT GCAGTGGGCC CGGGGTCCAG GGAGCCGCTA
AGGGTCCCTG AAGCTGTGGC CCTAGAGCGG CGGCGGGAGC AGGAAGAAAA GGAGGACATG
GAGACCCAGG CTGTGGCAAC GTCCCCCGAT GGCCGATACC TCAAGTTTGA CATCGAGATT
GGACGTGGCT CCTTCAAGAC GGTGTATCGA GGGCTAGACA CCGACACCAC AGTGGAGGTG
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTAGAGCTG AGCGGCAGCG CTTCTCAGAG
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA TGATTCGTGG
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
ACGCTCAAGA CGTACCTGAG GCGGTTCCGG GAGATGAAGC CGCGGGTCCT TCAGCGCTGG
AGCCGCCAAA TCCTGCGGGG ACTTCATTTC CTACACTCCC GGGTTCCTCC CATCCTGCAC
CGGGATCTCA AGTGCGACAA TGTCTTTATC ACGGGACCTA CTGGCTCTGT CAAAATCGGG
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTTGCCA AGAGTGTCAT CGGGACCCCG
GAATTCATGG CCCCCGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
TTCGGCATGT GCATGCTGGA GATGGCCACC TCTGAGTACC CGTACTCCGA GTGCCAGAAT
GCCGCGCAAA TCTACCGCAA GGTCACTTCG GGCAGAAAGC CGAACAGCTT CCACAAGGTG
AAGATACCCG AGGTGAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAACGAGAGG
TTCACCATCC AGGACCTCCT GGCCCACGCC TTCTTCCGCG AGGAGCGCGG TGTGCACGTG
GAACTAGCGG AGGAGGACGA CGGCGAGAAG CCGGGCCTCA AGCTCTGGCT GCGCATGGAG
GACGCGCGGC GCGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCCT GTTCCAGCTG
GGCCGGGACG CGGCCGAGGA GGTGGCACAG GAGATGGTGG CTCTGGGCTT GGTCTGTGAA
GCCGATTACC AGCCAGTGGC CCGTGCAGTA CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
CGTGAGAAGC TGCGTAAAGC AAGGGAATTG GAGGCACTCC CACCAGAGCC AGGACCTCCA
CCAGCAACTG TGCCCATGGC CCCCGGTCCC CCCAGTGTCT TCCCCCCTGA GCCTGAGGAG
CCAGAGGCAG ACCAGCACCA GCCCTTCCTT TTCCGCCACG CCAGCTACTC ATCTACCACT
TCGGATTGCG AGACTGATGG CTACCTCAGC TCCTCCGGCT TCCTGGATGC CTCAGACCCT
GCCCTTCAGC CCCCTGGGGG GGTGCCATCC AGCCTGGCTG AGTCCCATCT CTGCCTGCCC
TCGGCTTTTG CCCTATCCAT TCCACGTTCT GGCCCTGGAA GTGACTTTTC CCCCGGGGAC
AGCTATGCCT CAGATGCAGC TTCAGGCCTT AGCGATGTGG GAGAAGGGAT GGGACAAATG
AGGAGACCCC CAGGGAGGAA TCTCCGGCGC AGACCCCGAT CCCGGCTGCG GGTCACTAGT
GTCTCAGACC AGAATGACAG AGTGGTTGAG TGCCAGCTAC AGACCCATAA CAGCAAGATG
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCGGAAG AGATTGCAGC TGCCATGGTA
TATAACGAGT TCATTCTGCC TTCGGAGCGA GATGGATTTC TCAGACGGAT TCGGGAGATT
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GACACTGGCC CCATGGAGGC TGCTGAAGAC
ACCCTAAGCC CCCAGGAGGA GCCAGCACCA TTACCTGCCC TGCCCGTCCC CCTCCCAGAC
CCATCCAATG AAGAGCTCCA GAGCAGCACC TCCCTGGAGC ACAGGAGCTG GACAGCCTTC
TCCACCTCCT CATCTTCTCC TGGAACTCCT TTGTCTCCTG GAAACCCATT TTCCCCTGGA
ACCCCCATTT CCCCAGGTCC CATCTTCCCC ATCACTTCTC CCCCATGTCA TCCCAGCCCC
TCCCCATTCT CCCCCATTTC TTCCCAGGTC TCCTCAAATC CCTCTCCACA CCCCACCAGC
TCTCCACTTC CATTCTCCTC CAGCACACCC GAGTTTCCGG TCCCACTCTC TCAGTGTCCC
TGGAGTTCTC TCCCCACGAC TTCTCCACCT ACGTTCTCTC CCACTTGTTC TCAGGTCACT
CTTAGTTCCC CTTTCTTTCC TCCGTGCCCC TCCACTTCTT CCTTCCCCTC CACCACAGCA
GCCCCTCTCC TTTCTCTGGC TAGTGCCTTC TCACTGGCTG TGATGACTGT GGCCCAGTCC
CTGCTGTCCC CCTCACCTGG GCTCCTTTCC CAGTCTCCTC CAGCCCCTCC TAGTCCCCTC
CCTAGCCTGC CCCTTCCCCC TCCCGTTGCT CCTGGTGGCC AGGAAAGCCC TTCACCCCAC
ACAGCTGAGG TGGAGAGTGA GGCCTCACCA CCTCCTGCTC GGCCCCTCCC AGGGGAAGCC
AGGCTGGCGC CCATCTCTGA AGAGGGAAAG CCGCAGCTTG TTGGGCGTTT CCAAGTGACT
TCATCCAAGG AACCGGCTGA GCCTCTTCCC TTGCAGCCAA CATCCCCCAC TCTCTCTGGT
TCTCCAAAAC CTTCAACCCC TCAGCTCACT TCAGAGAGCT CAGATACAGA GGACAGTGCT
GGAGGCGGGC CAGAGACCAG GGAAGCTCTG GCTGAGAGCG ACCGTGCAGC TGAGGGTCTG
GGGGCTGGAG TTGAGGAGGA AGGAGATGAT GGGAAGGAAC CCCAAGTTGG GGGCAGCCCC
CAACCCCTGA GCCATCCCAG CCCAGTGTGG ATGAACTACT CCTACAGCAG CCTGTGTTTG
AGCAGCGAGG AGTCAGAAAG CAGTGGGGAA GATGAGGAGT TCTGGGCTGA GCTGCAGAGT
CTTCGGCAGA AGCACTTGTC AGAGGTGGAA ACACTACAGA CACTACAGAA AAAAGAAATT
GAAGATTTGT ACAGCCGGCT GGGGAAGCAG CCCCCACCGG GTATTGTGGC CCCAGCTGCT
ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC AAGGGCAGCT TCCCCACCTC CCGCCGCAAC
AGCCTACAGC GCTCTGAGCC CCCAGGCCCT GTGCTCTCCT TCCCCCATCC TGCTCCCAGG
CATCATGCGA AGGAACTCTC TGAGTGGCAG CAGCACCGGC TCCCAGGAGC AGCGGGCAAG
CAAGGGGGTG ACATTCGCCG GGGATGTTGG CAGGATGTGA ATTCAGAACA GAAGCCATGT
ATCTCCCCCA CACCAGGGCC CACCATGGAG CTTGTGTTCT CAGAATCTGA TGCTTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005257652.1
CDS1027..4866
Misc Feature(1)1540..2322(+)
Misc Feature(2)1561..2322(+)
Misc Feature(3)1564..2049(+)
Misc Feature(4)1564..1989(+)
Misc Feature(5)1576..2049(+)
Misc Feature(6)1984..2049(+)
Misc Feature(7)2383..2493(+)
Translation

Target ORF information:

RefSeq Version XM_005257595
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005257595

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGTTGGCAT CCCCGGCCAC GGAGACCACC GTCCTCATGT CCCAGACTGA GGCCGACCTG 
GCCCTGCGGC CCCCGCCTCC TCTTGGCACC GCGGGGCAGC CCCGCCTCGG GCCCCCTCCT
CGCCGAGCGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC TCGTCTCAGC
CGCCGTAGCT CAGTCGACTT GGGGCTGCTG AGCTCTTGGT CCCTGCCAGC CTCACCCGCT
CCGGACCCCC CCGATCCTCC GGACTCCGCT GGTCCTGGCC CCGCGAGGAG CCCACCGCCT
AGCTCCAAAG AACCCCCCGA GGGCACGTGG ACCGAGGGAG CCCCTGTGAA GGCTGCGGAA
GACTCCGCGC GTCCCGAGCT CCCGGACTCT GCAGTGGGCC CGGGGTCCAG GGAGCCGCTA
AGGGTCCCTG AAGCTGTGGC CCTAGAGCGG CGGCGGGAGC AGGAAGAAAA GGAGGACATG
GAGACCCAGG CTGTGGCAAC GTCCCCCGAT GGCCGATACC TCAAGTTTGA CATCGAGATT
GGACGTGGCT CCTTCAAGAC GGTGTATCGA GGGCTAGACA CCGACACCAC AGTGGAGGTG
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTAGAGCTG AGCGGCAGCG CTTCTCAGAG
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA TGATTCGTGG
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
ACGCTCAAGA CGTACCTGAG GCGGTTCCGG GAGATGAAGC CGCGGGTCCT TCAGCGCTGG
AGCCGCCAAA TCCTGCGGGG ACTTCATTTC CTACACTCCC GGGTTCCTCC CATCCTGCAC
CGGGATCTCA AGTGCGACAA TGTCTTTATC ACGGGACCTA CTGGCTCTGT CAAAATCGGG
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTTGCCA AGAGTGTCAT CGGGACCCCG
GAATTCATGG CCCCCGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
TTCGGCATGT GCATGCTGGA GATGGCCACC TCTGAGTACC CGTACTCCGA GTGCCAGAAT
GCCGCGCAAA TCTACCGCAA GGTCACTTCG GGCAGAAAGC CGAACAGCTT CCACAAGGTG
AAGATACCCG AGGTGAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAACGAGAGG
TTCACCATCC AGGACCTCCT GGCCCACGCC TTCTTCCGCG AGGAGCGCGG TGTGCACGTG
GAACTAGCGG AGGAGGACGA CGGCGAGAAG CCGGGCCTCA AGCTCTGGCT GCGCATGGAG
GACGCGCGGC GCGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCCT GTTCCAGCTG
GGCCGGGACG CGGCCGAGGA GGTGGCACAG GAGATGGTGG CTCTGGGCTT GGTCTGTGAA
GCCGATTACC AGCCAGTGGC CCGTGCAGTA CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
CGTGAGAAGC TGCGTAAAGC AAGGGAATTG GAGGCACTCC CACCAGAGCC AGGACCTCCA
CCAGCAACTG TGCCCATGGC CCCCGGTCCC CCCAGTGTCT TCCCCCCTGA GCCTGAGGAG
CCAGAGGCAG ACCAGCACCA GCCCTTCCTT TTCCGCCACG CCAGCTACTC ATCTACCACT
TCGGATTGCG AGACTGATGG CTACCTCAGC TCCTCCGGCT TCCTGGATGC CTCAGACCCT
GCCCTTCAGC CCCCTGGGGG GGTGCCATCC AGCCTGGCTG AGTCCCATCT CTGCCTGCCC
TCGGCTTTTG CCCTATCCAT TCCACGTTCT GGCCCTGGAA GTGACTTTTC CCCCGGGGAC
AGCTATGCCT CAGATGCAGC TTCAGGCCTT AGCGATGTGG GAGAAGGGAT GGGACAAATG
AGGAGACCCC CAGGGAGGAA TCTCCGGCGC AGACCCCGAT CCCGGCTGCG GGTCACTAGT
GTCTCAGACC AGAATGACAG AGTGGTTGAG TGCCAGCTAC AGACCCATAA CAGCAAGATG
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCGGAAG AGATTGCAGC TGCCATGGTA
TATAACGAGT TCATTCTGCC TTCGGAGCGA GATGGATTTC TCAGACGGAT TCGGGAGATT
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GACACTGGCC CCATGGAGGC TGCTGAAGAC
ACCCTAAGCC CCCAGGAGGA GCCAGCACCA TTACCTGCCC TGCCCGTCCC CCTCCCAGAC
CCATCCAATG AAGAGCTCCA GAGCAGCACC TCCCTGGAGC ACAGGAGCTG GACAGCCTTC
TCCACCTCCT CATCTTCTCC TGGAACTCCT TTGTCTCCTG GAAACCCATT TTCCCCTGGA
ACCCCCATTT CCCCAGGTCC CATCTTCCCC ATCACTTCTC CCCCATGTCA TCCCAGCCCC
TCCCCATTCT CCCCCATTTC TTCCCAGGTC TCCTCAAATC CCTCTCCACA CCCCACCAGC
TCTCCACTTC CATTCTCCTC CAGCACACCC GAGTTTCCGG TCCCACTCTC TCAGTGTCCC
TGGAGTTCTC TCCCCACGAC TTCTCCACCT ACGTTCTCTC CCACTTGTTC TCAGGTCACT
CTTAGTTCCC CTTTCTTTCC TCCGTGCCCC TCCACTTCTT CCTTCCCCTC CACCACAGCA
GCCCCTCTCC TTTCTCTGGC TAGTGCCTTC TCACTGGCTG TGATGACTGT GGCCCAGTCC
CTGCTGTCCC CCTCACCTGG GCTCCTTTCC CAGTCTCCTC CAGCCCCTCC TAGTCCCCTC
CCTAGCCTGC CCCTTCCCCC TCCCGTTGCT CCTGGTGGCC AGGAAAGCCC TTCACCCCAC
ACAGCTGAGG TGGAGAGTGA GGCCTCACCA CCTCCTGCTC GGCCCCTCCC AGGGGAAGCC
AGGCTGGCGC CCATCTCTGA AGAGGGAAAG CCGCAGCTTG TTGGGCGTTT CCAAGTGACT
TCATCCAAGG AACCGGCTGA GCCTCTTCCC TTGCAGCCAA CATCCCCCAC TCTCTCTGGT
TCTCCAAAAC CTTCAACCCC TCAGCTCACT TCAGAGAGCT CAGATACAGA GGACAGTGCT
GGAGGCGGGC CAGAGACCAG GGAAGCTCTG GCTGAGAGCG ACCGTGCAGC TGAGGGTCTG
GGGGCTGGAG TTGAGGAGGA AGGAGATGAT GGGAAGGAAC CCCAAGTTGG GGGCAGCCCC
CAACCCCTGA GCCATCCCAG CCCAGTGTGG ATGAACTACT CCTACAGCAG CCTGTGTTTG
AGCAGCGAGG AGTCAGAAAG CAGTGGGGAA GATGAGGAGT TCTGGGCTGA GCTGCAGAGT
CTTCGGCAGA AGCACTTGTC AGAGGTGGAA ACACTACAGA CACTACAGAA AAAAGAAATT
GAAGATTTGT ACAGCCGGCT GGGGAAGCAG CCCCCACCGG GTATTGTGGC CCCAGCTGCT
ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC AAGGGCAGCT TCCCCACCTC CCGCCGCAAC
AGCCTACAGC GCTCTGAGCC CCCAGGCCCT GTGCTCTCCT TCCCCCATCC TGCTCCCAGG
CATCATGCGA AGGAACTCTC TGAGTGGCAG CAGCACCGGC TCCCAGGAGC AGCGGGCAAG
CAAGGGGGTG ACATTCGCCG GGGATGTTGG CAGGATGTGA ATTCAGAACA GAAGCCATGT
ATCTCCCCCA CACCAGGGCC CACCATGGAG CTTGTGTTCT CAGAATCTGA TGCTTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71641
Clone ID Related Accession (Same CDS sequence) XM_011525132
Accession Version XM_011525132.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3837bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product serine/threonine-protein kinase WNK4 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGTTGGCAT CCCCGGCCAC GGAGACCACC GTCCTCATGT CCCAGACTGA GGCCGACCTG 
GCCCTGCGGC CCCCGCCTCC TCTTGGCACC GCGGGGCAGC CCCGCCTCGG GCCCCCTCCT
CGCCGAGCGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC TCGTCTCAGC
CGCCGTAGCT CAGTCGACTT GGGGCTGCTG AGCTCTTGGT CCCTGCCAGC CTCACCCGCT
CCGGACCCCC CCGATCCTCC GGACTCCGCT GGTCCTGGCC CCGCGAGGAG CCCACCGCCT
AGCTCCAAAG AACCCCCCGA GGGCACGTGG ACCGAGGGAG CCCCTGTGAA GGCTGCGGAA
GACTCCGCGC GTCCCGAGCT CCCGGACTCT GCAGTGGGCC CGGGGTCCAG GGAGCCGCTA
AGGGTCCCTG AAGCTGTGGC CCTAGAGCGG CGGCGGGAGC AGGAAGAAAA GGAGGACATG
GAGACCCAGG CTGTGGCAAC GTCCCCCGAT GGCCGATACC TCAAGTTTGA CATCGAGATT
GGACGTGGCT CCTTCAAGAC GGTGTATCGA GGGCTAGACA CCGACACCAC AGTGGAGGTG
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTAGAGCTG AGCGGCAGCG CTTCTCAGAG
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA TGATTCGTGG
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
ACGCTCAAGA CGTACCTGAG GCGGTTCCGG GAGATGAAGC CGCGGGTCCT TCAGCGCTGG
AGCCGCCAAA TCCTGCGGGG ACTTCATTTC CTACACTCCC GGGTTCCTCC CATCCTGCAC
CGGGATCTCA AGTGCGACAA TGTCTTTATC ACGGGACCTA CTGGCTCTGT CAAAATCGGG
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTTGCCA AGAGTGTCAT CGGGACCCCG
GAATTCATGG CCCCCGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
TTCGGCATGT GCATGCTGGA GATGGCCACC TCTGAGTACC CGTACTCCGA GTGCCAGAAT
GCCGCGCAAA TCTACCGCAA GGTCACTTCG GGCAGAAAGC CGAACAGCTT CCACAAGGTG
AAGATACCCG AGGTGAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAACGAGAGG
TTCACCATCC AGGACCTCCT GGCCCACGCC TTCTTCCGCG AGGAGCGCGG TGTGCACGTG
GAACTAGCGG AGGAGGACGA CGGCGAGAAG CCGGGCCTCA AGCTCTGGCT GCGCATGGAG
GACGCGCGGC GCGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCCT GTTCCAGCTG
GGCCGGGACG CGGCCGAGGA GGTGGCACAG GAGATGGTGG CTCTGGGCTT GGTCTGTGAA
GCCGATTACC AGCCAGTGGC CCGTGCAGTA CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
CGTGAGAAGC TGCGTAAAGC AAGGGAATTG GAGGCACTCC CACCAGAGCC AGGACCTCCA
CCAGCAACTG TGCCCATGGC CCCCGGTCCC CCCAGTGTCT TCCCCCCTGA GCCTGAGGAG
CCAGAGGCAG ACCAGCACCA GCCCTTCCTT TTCCGCCACG CCAGCTACTC ATCTACCACT
TCGGATTGCG AGACTGATGG CTACCTCAGC TCCTCCGGCT TCCTGGATGC CTCAGACCCT
GCCCTTCAGC CCCCTGGGGG GGTGCCATCC AGCCTGGCTG AGTCCCATCT CTGCCTGCCC
TCGGCTTTTG CCCTATCCAT TCCACGTTCT GGCCCTGGAA GTGACTTTTC CCCCGGGGAC
AGCTATGCCT CAGATGCAGC TTCAGGCCTT AGCGATGTGG GAGAAGGGAT GGGACAAATG
AGGAGACCCC CAGGGAGGAA TCTCCGGCGC AGACCCCGAT CCCGGCTGCG GGTCACTAGT
GTCTCAGACC AGAATGACAG AGTGGTTGAG TGCCAGCTAC AGACCCATAA CAGCAAGATG
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCGGAAG AGATTGCAGC TGCCATGGTA
TATAACGAGT TCATTCTGCC TTCGGAGCGA GATGGATTTC TCAGACGGAT TCGGGAGATT
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GACACTGGCC CCATGGAGGC TGCTGAAGAC
ACCCTAAGCC CCCAGGAGGA GCCAGCACCA TTACCTGCCC TGCCCGTCCC CCTCCCAGAC
CCATCCAATG AGCTCCAGAG CAGCACCTCC CTGGAGCACA GGAGCTGGAC AGCCTTCTCC
ACCTCCTCAT CTTCTCCTGG AACTCCTTTG TCTCCTGGAA ACCCATTTTC CCCTGGAACC
CCCATTTCCC CAGGTCCCAT CTTCCCCATC ACTTCTCCCC CATGTCATCC CAGCCCCTCC
CCATTCTCCC CCATTTCTTC CCAGGTCTCC TCAAATCCCT CTCCACACCC CACCAGCTCT
CCACTTCCAT TCTCCTCCAG CACACCCGAG TTTCCGGTCC CACTCTCTCA GTGTCCCTGG
AGTTCTCTCC CCACGACTTC TCCACCTACG TTCTCTCCCA CTTGTTCTCA GGTCACTCTT
AGTTCCCCTT TCTTTCCTCC GTGCCCCTCC ACTTCTTCCT TCCCCTCCAC CACAGCAGCC
CCTCTCCTTT CTCTGGCTAG TGCCTTCTCA CTGGCTGTGA TGACTGTGGC CCAGTCCCTG
CTGTCCCCCT CACCTGGGCT CCTTTCCCAG TCTCCTCCAG CCCCTCCTAG TCCCCTCCCT
AGCCTGCCCC TTCCCCCTCC CGTTGCTCCT GGTGGCCAGG AAAGCCCTTC ACCCCACACA
GCTGAGGTGG AGAGTGAGGC CTCACCACCT CCTGCTCGGC CCCTCCCAGG GGAAGCCAGG
CTGGCGCCCA TCTCTGAAGA GGGAAAGCCG CAGCTTGTTG GGCGTTTCCA AGTGACTTCA
TCCAAGGAAC CGGCTGAGCC TCTTCCCTTG CAGCCAACAT CCCCCACTCT CTCTGGTTCT
CCAAAACCTT CAACCCCTCA GCTCACTTCA GAGAGCTCAG ATACAGAGGA CAGTGCTGGA
GGCGGGCCAG AGACCAGGGA AGCTCTGGCT GAGAGCGACC GTGCAGCTGA GGGTCTGGGG
GCTGGAGTTG AGGAGGAAGG AGATGATGGG AAGGAACCCC AAGTTGGGGG CAGCCCCCAA
CCCCTGAGCC ATCCCAGCCC AGTGTGGATG AACTACTCCT ACAGCAGCCT GTGTTTGAGC
AGCGAGGAGT CAGAAAGCAG TGGGGAAGAT GAGGAGTTCT GGGCTGAGCT GCAGAGTCTT
CGGCAGAAGC ACTTGTCAGA GGTGGAAACA CTACAGACAC TACAGAAAAA AGAAATTGAA
GATTTGTACA GCCGGCTGGG GAAGCAGCCC CCACCGGGTA TTGTGGCCCC AGCTGCTATG
CTGTCCAGCC GCCAGCGCCG CCTCTCCAAG GGCAGCTTCC CCACCTCCCG CCGCAACAGC
CTACAGCGCT CTGAGCCCCC AGGCCCTGTG CTCTCCTTCC CCCATCCTGC TCCCAGGCAT
CATGCGAAGG AACTCTCTGA GTGGCAGCAG CACCGGCTCC CAGGAGCAGC GGGCAAGCAA
GGGGGTGACA TTCGCCGGGG ATGTTGGCAG GATGTGAATT CAGAACAGAA GCCATGTATC
TCCCCCACAC CAGGGCCCAC CATGGAGCTT GTGTTCTCAG AATCTGATGC TTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011523434.1
CDS1027..4863
Misc Feature(1)1540..2322(+)
Misc Feature(2)1561..2322(+)
Misc Feature(3)1564..2049(+)
Misc Feature(4)1564..1989(+)
Misc Feature(5)1576..2049(+)
Misc Feature(6)1984..2049(+)
Misc Feature(7)2383..2493(+)
Translation

Target ORF information:

RefSeq Version XM_011525132
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011525132

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGTTGGCAT CCCCGGCCAC GGAGACCACC GTCCTCATGT CCCAGACTGA GGCCGACCTG 
GCCCTGCGGC CCCCGCCTCC TCTTGGCACC GCGGGGCAGC CCCGCCTCGG GCCCCCTCCT
CGCCGAGCGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC TCGTCTCAGC
CGCCGTAGCT CAGTCGACTT GGGGCTGCTG AGCTCTTGGT CCCTGCCAGC CTCACCCGCT
CCGGACCCCC CCGATCCTCC GGACTCCGCT GGTCCTGGCC CCGCGAGGAG CCCACCGCCT
AGCTCCAAAG AACCCCCCGA GGGCACGTGG ACCGAGGGAG CCCCTGTGAA GGCTGCGGAA
GACTCCGCGC GTCCCGAGCT CCCGGACTCT GCAGTGGGCC CGGGGTCCAG GGAGCCGCTA
AGGGTCCCTG AAGCTGTGGC CCTAGAGCGG CGGCGGGAGC AGGAAGAAAA GGAGGACATG
GAGACCCAGG CTGTGGCAAC GTCCCCCGAT GGCCGATACC TCAAGTTTGA CATCGAGATT
GGACGTGGCT CCTTCAAGAC GGTGTATCGA GGGCTAGACA CCGACACCAC AGTGGAGGTG
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTAGAGCTG AGCGGCAGCG CTTCTCAGAG
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA TGATTCGTGG
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
ACGCTCAAGA CGTACCTGAG GCGGTTCCGG GAGATGAAGC CGCGGGTCCT TCAGCGCTGG
AGCCGCCAAA TCCTGCGGGG ACTTCATTTC CTACACTCCC GGGTTCCTCC CATCCTGCAC
CGGGATCTCA AGTGCGACAA TGTCTTTATC ACGGGACCTA CTGGCTCTGT CAAAATCGGG
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTTGCCA AGAGTGTCAT CGGGACCCCG
GAATTCATGG CCCCCGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
TTCGGCATGT GCATGCTGGA GATGGCCACC TCTGAGTACC CGTACTCCGA GTGCCAGAAT
GCCGCGCAAA TCTACCGCAA GGTCACTTCG GGCAGAAAGC CGAACAGCTT CCACAAGGTG
AAGATACCCG AGGTGAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAACGAGAGG
TTCACCATCC AGGACCTCCT GGCCCACGCC TTCTTCCGCG AGGAGCGCGG TGTGCACGTG
GAACTAGCGG AGGAGGACGA CGGCGAGAAG CCGGGCCTCA AGCTCTGGCT GCGCATGGAG
GACGCGCGGC GCGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCCT GTTCCAGCTG
GGCCGGGACG CGGCCGAGGA GGTGGCACAG GAGATGGTGG CTCTGGGCTT GGTCTGTGAA
GCCGATTACC AGCCAGTGGC CCGTGCAGTA CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
CGTGAGAAGC TGCGTAAAGC AAGGGAATTG GAGGCACTCC CACCAGAGCC AGGACCTCCA
CCAGCAACTG TGCCCATGGC CCCCGGTCCC CCCAGTGTCT TCCCCCCTGA GCCTGAGGAG
CCAGAGGCAG ACCAGCACCA GCCCTTCCTT TTCCGCCACG CCAGCTACTC ATCTACCACT
TCGGATTGCG AGACTGATGG CTACCTCAGC TCCTCCGGCT TCCTGGATGC CTCAGACCCT
GCCCTTCAGC CCCCTGGGGG GGTGCCATCC AGCCTGGCTG AGTCCCATCT CTGCCTGCCC
TCGGCTTTTG CCCTATCCAT TCCACGTTCT GGCCCTGGAA GTGACTTTTC CCCCGGGGAC
AGCTATGCCT CAGATGCAGC TTCAGGCCTT AGCGATGTGG GAGAAGGGAT GGGACAAATG
AGGAGACCCC CAGGGAGGAA TCTCCGGCGC AGACCCCGAT CCCGGCTGCG GGTCACTAGT
GTCTCAGACC AGAATGACAG AGTGGTTGAG TGCCAGCTAC AGACCCATAA CAGCAAGATG
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCGGAAG AGATTGCAGC TGCCATGGTA
TATAACGAGT TCATTCTGCC TTCGGAGCGA GATGGATTTC TCAGACGGAT TCGGGAGATT
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GACACTGGCC CCATGGAGGC TGCTGAAGAC
ACCCTAAGCC CCCAGGAGGA GCCAGCACCA TTACCTGCCC TGCCCGTCCC CCTCCCAGAC
CCATCCAATG AGCTCCAGAG CAGCACCTCC CTGGAGCACA GGAGCTGGAC AGCCTTCTCC
ACCTCCTCAT CTTCTCCTGG AACTCCTTTG TCTCCTGGAA ACCCATTTTC CCCTGGAACC
CCCATTTCCC CAGGTCCCAT CTTCCCCATC ACTTCTCCCC CATGTCATCC CAGCCCCTCC
CCATTCTCCC CCATTTCTTC CCAGGTCTCC TCAAATCCCT CTCCACACCC CACCAGCTCT
CCACTTCCAT TCTCCTCCAG CACACCCGAG TTTCCGGTCC CACTCTCTCA GTGTCCCTGG
AGTTCTCTCC CCACGACTTC TCCACCTACG TTCTCTCCCA CTTGTTCTCA GGTCACTCTT
AGTTCCCCTT TCTTTCCTCC GTGCCCCTCC ACTTCTTCCT TCCCCTCCAC CACAGCAGCC
CCTCTCCTTT CTCTGGCTAG TGCCTTCTCA CTGGCTGTGA TGACTGTGGC CCAGTCCCTG
CTGTCCCCCT CACCTGGGCT CCTTTCCCAG TCTCCTCCAG CCCCTCCTAG TCCCCTCCCT
AGCCTGCCCC TTCCCCCTCC CGTTGCTCCT GGTGGCCAGG AAAGCCCTTC ACCCCACACA
GCTGAGGTGG AGAGTGAGGC CTCACCACCT CCTGCTCGGC CCCTCCCAGG GGAAGCCAGG
CTGGCGCCCA TCTCTGAAGA GGGAAAGCCG CAGCTTGTTG GGCGTTTCCA AGTGACTTCA
TCCAAGGAAC CGGCTGAGCC TCTTCCCTTG CAGCCAACAT CCCCCACTCT CTCTGGTTCT
CCAAAACCTT CAACCCCTCA GCTCACTTCA GAGAGCTCAG ATACAGAGGA CAGTGCTGGA
GGCGGGCCAG AGACCAGGGA AGCTCTGGCT GAGAGCGACC GTGCAGCTGA GGGTCTGGGG
GCTGGAGTTG AGGAGGAAGG AGATGATGGG AAGGAACCCC AAGTTGGGGG CAGCCCCCAA
CCCCTGAGCC ATCCCAGCCC AGTGTGGATG AACTACTCCT ACAGCAGCCT GTGTTTGAGC
AGCGAGGAGT CAGAAAGCAG TGGGGAAGAT GAGGAGTTCT GGGCTGAGCT GCAGAGTCTT
CGGCAGAAGC ACTTGTCAGA GGTGGAAACA CTACAGACAC TACAGAAAAA AGAAATTGAA
GATTTGTACA GCCGGCTGGG GAAGCAGCCC CCACCGGGTA TTGTGGCCCC AGCTGCTATG
CTGTCCAGCC GCCAGCGCCG CCTCTCCAAG GGCAGCTTCC CCACCTCCCG CCGCAACAGC
CTACAGCGCT CTGAGCCCCC AGGCCCTGTG CTCTCCTTCC CCCATCCTGC TCCCAGGCAT
CATGCGAAGG AACTCTCTGA GTGGCAGCAG CACCGGCTCC CAGGAGCAGC GGGCAAGCAA
GGGGGTGACA TTCGCCGGGG ATGTTGGCAG GATGTGAATT CAGAACAGAA GCCATGTATC
TCCCCCACAC CAGGGCCCAC CATGGAGCTT GTGTTCTCAG AATCTGATGC TTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71642
Clone ID Related Accession (Same CDS sequence) XM_011525133
Accession Version XM_011525133.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3798bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product serine/threonine-protein kinase WNK4 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGTTGGCAT CCCCGGCCAC GGAGACCACC GTCCTCATGT CCCAGACTGA GGCCGACCTG 
GCCCTGCGGC CCCCGCCTCC TCTTGGCACC GCGGGGCAGC CCCGCCTCGG GCCCCCTCCT
CGCCGAGCGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC TCGTCTCAGC
CGCCGTAGCT CAGTCGACTT GGGGCTGCTG AGCTCTTGGT CCCTGCCAGC CTCACCCGCT
CCGGACCCCC CCGATCCTCC GGACTCCGCT GGTCCTGGCC CCGCGAGGAG CCCACCGCCT
AGCTCCAAAG AACCCCCCGA GGGCACGTGG ACCGAGGGAG CCCCTGTGAA GGCTGCGGAA
GACTCCGCGC GTCCCGAGCT CCCGGACTCT GCAGTGGGCC CGGGGTCCAG GGAGCCGCTA
AGGGTCCCTG AAGCTGTGGC CCTAGAGCGG CGGCGGGAGC AGGAAGAAAA GGAGGACATG
GAGACCCAGG CTGTGGCAAC GTCCCCCGAT GGCCGATACC TCAAGTTTGA CATCGAGATT
GGACGTGGCT CCTTCAAGAC GGTGTATCGA GGGCTAGACA CCGACACCAC AGTGGAGGTG
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTAGAGCTG AGCGGCAGCG CTTCTCAGAG
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA TGATTCGTGG
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
ACGCTCAAGA CGTACCTGAG GCGGTTCCGG GAGATGAAGC CGCGGGTCCT TCAGCGCTGG
AGCCGCCAAA TCCTGCGGGG ACTTCATTTC CTACACTCCC GGGTTCCTCC CATCCTGCAC
CGGGATCTCA AGTGCGACAA TGTCTTTATC ACGGGACCTA CTGGCTCTGT CAAAATCGGG
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTTGCCA AGAGTGTCAT CGGGACCCCG
GAATTCATGG CCCCCGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
TTCGGCATGT GCATGCTGGA GATGGCCACC TCTGAGTACC CGTACTCCGA GTGCCAGAAT
GCCGCGCAAA TCTACCGCAA GGTCACTTCG GGCAGAAAGC CGAACAGCTT CCACAAGGTG
AAGATACCCG AGGTGAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAACGAGAGG
TTCACCATCC AGGACCTCCT GGCCCACGCC TTCTTCCGCG AGGAGCGCGG TGTGCACGTG
GAACTAGCGG AGGAGGACGA CGGCGAGAAG CCGGGCCTCA AGCTCTGGCT GCGCATGGAG
GACGCGCGGC GCGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCCT GTTCCAGCTG
GGCCGGGACG CGGCCGAGGA GGTGGCACAG GAGATGGTGG CTCTGGGCTT GGTCTGTGAA
GCCGATTACC AGCCAGTGGC CCGTGCAGTA CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
CGTGAGAAGC TGCGTAAAGC AAGGGAATTG GAGGCACTCC CACCAGAGCC AGGACCTCCA
CCAGCAACTG TGCCCATGGC CCCCGGTCCC CCCAGTGTCT TCCCCCCTGA GCCTGAGGAG
CCAGAGGCAG ACCAGCACCA GCCCTTCCTT TTCCGCCACG CCAGCTACTC ATCTACCACT
TCGGATTGCG AGACTGATGG CTACCTCAGC TCCTCCGGCT TCCTGGATGC CTCAGACCCT
GCCCTTCAGC CCCCTGGGGG GGTGCCATCC AGCCTGGCTG AGTCCCATCT CTGCCTGCCC
TCGGCTTTTG CCCTATCCAT TCCACGTTCT GGCCCTGGAA GTGACTTTTC CCCCGGGGAC
AGCTATGCCT CAGATGCAGC TTCAGGCCTT AGCGATGTGG GAGAAGGGAT GGGACAAATG
AGGAGACCCC CAGGGAGGAA TCTCCGGCGC AGACCCCGAT CCCGGCTGCG GGTCACTAGT
GTCTCAGACC AGAATGACAG AGTGGTTGAG TGCCAGCTAC AGACCCATAA CAGCAAGATG
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCGGAAG AGATTGCAGC TGCCATGGTA
TATAACGAGT TCATTCTGCC TTCGGAGCGA GATGGATTTC TCAGACGGAT TCGGGAGATT
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GACACTGGCC CCATGGAGGC TGCTGAAGAC
ACCCTAAGCC CCCAGGAGGA GCCAGCACCA TTACCTGCCC TGCCCGTCCC CCTCCCAGAC
CCATCCAATG AAGAGCTCCA GAGCAGCACC TCCCTGGAGC ACAGGAGCTG GACAGCCTTC
TCCACCTCCT CATCTTCTCC TGGAACTCCT TTGTCTCCTG GAAACCCATT TTCCCCTGGA
ACCCCCATTT CCCCAGGTCC CATCTTCCCC ATCACTTCTC CCCCATGTCA TCCCAGCCCC
TCCCCATTCT CCCCCATTTC TTCCCAGGTC TCCTCAAATC CCTCTCCACA CCCCACCAGC
TCTCCACTTC CATTCTCCTC CAGCACACCC GAGTTTCCGG TCCCACTCTC TCAGTGTCCC
TGGAGTTCTC TCCCCACGAC TTCTCCACCT ACGTTCTCTC CCACTTGTTC TCAGGTCACT
CTTAGTTCCC CTTTCTTTCC TCCGTGCCCC TCCACTTCTT CCTTCCCCTC CACCACAGCA
GCCCCTCTCC TTTCTCTGGC TAGTGCCTTC TCACTGGCTG TGATGACTGT GGCCCAGTCC
CTGCTGTCCC CCTCACCTGG GCTCCTTTCC CAGTCTCCTC CAGCCCCTCC TAGTCCCCTC
CCTAGCCTGC CCCTTCCCCC TCCCGTTGCT CCTGGTGGCC AGGAAAGCCC TTCACCCCAC
ACAGCTGAGG TGGAGAGTGA GGCCTCACCA CCTCCTGCTC GGCCCCTCCC AGGGGAAGCC
AGGCTGGCGC CCATCTCTGA AGAGGGAAAG CCGCAGCTTG TTGGGCGTTT CCAAGTGACT
TCATCCAAGG AACCGGCTGA GCCTCTTCCC TTGCAGCCAA CATCCCCCAC TCTCTCTGGT
TCTCCAAAAC CTTCAACCCC TCAGCTCACT TCAGAGAGCT CAGATACAGA GGACAGTGCT
GGAGGCGGGC CAGAGACCAG GGAAGCTCTG GCTGAGAGCG ACCGTGCAGC TGAGGGTCTG
GGGGCTGGAG TTGAGGAGGA AGGAGATGAT GGGAAGGAAC CCCAAGTTGG GGGCAGCCCC
CAACCCCTGA GCCATCCCAG CCCAGTGTGG ATGAACTACT CCTACAGCAG CCTGTGTTTG
AGCAGCGAGG AGTCAGAAAG CAGTGGGGAA GATGAGGAGT TCTGGGCTGA GCTGCAGAGT
CTTCGGCAGA AGCACTTGTC AGAGGTGGAA ACACTACAGA CACTACAGAA AAAAGAAATT
GAAGATTTGT ACAGCCGGCT GGGGAAGCAG CCCCCACCGG GTATTGTGGC CCCAGCTGCT
ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC AAGGGCAGCT TCCCCACCTC CCGCCGCAAC
AGCCTACAGC GCTCTGAGCC CCCAGGCCCT GTGCTCTCCT TCCCCCATCC TGCTCCCAGG
CATCATGCGA AGGAACTCTC TGAGTGGCAG CAGCACCGGC TCCCAGGAGC AGCGGTGAAT
TCAGAACAGA AGCCATGTAT CTCCCCCACA CCAGGGCCCA CCATGGAGCT TGTGTTCTCA
GAATCTGATG CTTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011523435.1
CDS1027..4824
Misc Feature(1)1540..2322(+)
Misc Feature(2)1561..2322(+)
Misc Feature(3)1564..2049(+)
Misc Feature(4)1564..1989(+)
Misc Feature(5)1576..2049(+)
Misc Feature(6)1984..2049(+)
Misc Feature(7)2383..2493(+)
Translation

Target ORF information:

RefSeq Version XM_011525133
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011525133

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGTTGGCAT CCCCGGCCAC GGAGACCACC GTCCTCATGT CCCAGACTGA GGCCGACCTG 
GCCCTGCGGC CCCCGCCTCC TCTTGGCACC GCGGGGCAGC CCCGCCTCGG GCCCCCTCCT
CGCCGAGCGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC TCGTCTCAGC
CGCCGTAGCT CAGTCGACTT GGGGCTGCTG AGCTCTTGGT CCCTGCCAGC CTCACCCGCT
CCGGACCCCC CCGATCCTCC GGACTCCGCT GGTCCTGGCC CCGCGAGGAG CCCACCGCCT
AGCTCCAAAG AACCCCCCGA GGGCACGTGG ACCGAGGGAG CCCCTGTGAA GGCTGCGGAA
GACTCCGCGC GTCCCGAGCT CCCGGACTCT GCAGTGGGCC CGGGGTCCAG GGAGCCGCTA
AGGGTCCCTG AAGCTGTGGC CCTAGAGCGG CGGCGGGAGC AGGAAGAAAA GGAGGACATG
GAGACCCAGG CTGTGGCAAC GTCCCCCGAT GGCCGATACC TCAAGTTTGA CATCGAGATT
GGACGTGGCT CCTTCAAGAC GGTGTATCGA GGGCTAGACA CCGACACCAC AGTGGAGGTG
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTAGAGCTG AGCGGCAGCG CTTCTCAGAG
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA TGATTCGTGG
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
ACGCTCAAGA CGTACCTGAG GCGGTTCCGG GAGATGAAGC CGCGGGTCCT TCAGCGCTGG
AGCCGCCAAA TCCTGCGGGG ACTTCATTTC CTACACTCCC GGGTTCCTCC CATCCTGCAC
CGGGATCTCA AGTGCGACAA TGTCTTTATC ACGGGACCTA CTGGCTCTGT CAAAATCGGG
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTTGCCA AGAGTGTCAT CGGGACCCCG
GAATTCATGG CCCCCGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
TTCGGCATGT GCATGCTGGA GATGGCCACC TCTGAGTACC CGTACTCCGA GTGCCAGAAT
GCCGCGCAAA TCTACCGCAA GGTCACTTCG GGCAGAAAGC CGAACAGCTT CCACAAGGTG
AAGATACCCG AGGTGAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAACGAGAGG
TTCACCATCC AGGACCTCCT GGCCCACGCC TTCTTCCGCG AGGAGCGCGG TGTGCACGTG
GAACTAGCGG AGGAGGACGA CGGCGAGAAG CCGGGCCTCA AGCTCTGGCT GCGCATGGAG
GACGCGCGGC GCGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCCT GTTCCAGCTG
GGCCGGGACG CGGCCGAGGA GGTGGCACAG GAGATGGTGG CTCTGGGCTT GGTCTGTGAA
GCCGATTACC AGCCAGTGGC CCGTGCAGTA CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
CGTGAGAAGC TGCGTAAAGC AAGGGAATTG GAGGCACTCC CACCAGAGCC AGGACCTCCA
CCAGCAACTG TGCCCATGGC CCCCGGTCCC CCCAGTGTCT TCCCCCCTGA GCCTGAGGAG
CCAGAGGCAG ACCAGCACCA GCCCTTCCTT TTCCGCCACG CCAGCTACTC ATCTACCACT
TCGGATTGCG AGACTGATGG CTACCTCAGC TCCTCCGGCT TCCTGGATGC CTCAGACCCT
GCCCTTCAGC CCCCTGGGGG GGTGCCATCC AGCCTGGCTG AGTCCCATCT CTGCCTGCCC
TCGGCTTTTG CCCTATCCAT TCCACGTTCT GGCCCTGGAA GTGACTTTTC CCCCGGGGAC
AGCTATGCCT CAGATGCAGC TTCAGGCCTT AGCGATGTGG GAGAAGGGAT GGGACAAATG
AGGAGACCCC CAGGGAGGAA TCTCCGGCGC AGACCCCGAT CCCGGCTGCG GGTCACTAGT
GTCTCAGACC AGAATGACAG AGTGGTTGAG TGCCAGCTAC AGACCCATAA CAGCAAGATG
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCGGAAG AGATTGCAGC TGCCATGGTA
TATAACGAGT TCATTCTGCC TTCGGAGCGA GATGGATTTC TCAGACGGAT TCGGGAGATT
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GACACTGGCC CCATGGAGGC TGCTGAAGAC
ACCCTAAGCC CCCAGGAGGA GCCAGCACCA TTACCTGCCC TGCCCGTCCC CCTCCCAGAC
CCATCCAATG AAGAGCTCCA GAGCAGCACC TCCCTGGAGC ACAGGAGCTG GACAGCCTTC
TCCACCTCCT CATCTTCTCC TGGAACTCCT TTGTCTCCTG GAAACCCATT TTCCCCTGGA
ACCCCCATTT CCCCAGGTCC CATCTTCCCC ATCACTTCTC CCCCATGTCA TCCCAGCCCC
TCCCCATTCT CCCCCATTTC TTCCCAGGTC TCCTCAAATC CCTCTCCACA CCCCACCAGC
TCTCCACTTC CATTCTCCTC CAGCACACCC GAGTTTCCGG TCCCACTCTC TCAGTGTCCC
TGGAGTTCTC TCCCCACGAC TTCTCCACCT ACGTTCTCTC CCACTTGTTC TCAGGTCACT
CTTAGTTCCC CTTTCTTTCC TCCGTGCCCC TCCACTTCTT CCTTCCCCTC CACCACAGCA
GCCCCTCTCC TTTCTCTGGC TAGTGCCTTC TCACTGGCTG TGATGACTGT GGCCCAGTCC
CTGCTGTCCC CCTCACCTGG GCTCCTTTCC CAGTCTCCTC CAGCCCCTCC TAGTCCCCTC
CCTAGCCTGC CCCTTCCCCC TCCCGTTGCT CCTGGTGGCC AGGAAAGCCC TTCACCCCAC
ACAGCTGAGG TGGAGAGTGA GGCCTCACCA CCTCCTGCTC GGCCCCTCCC AGGGGAAGCC
AGGCTGGCGC CCATCTCTGA AGAGGGAAAG CCGCAGCTTG TTGGGCGTTT CCAAGTGACT
TCATCCAAGG AACCGGCTGA GCCTCTTCCC TTGCAGCCAA CATCCCCCAC TCTCTCTGGT
TCTCCAAAAC CTTCAACCCC TCAGCTCACT TCAGAGAGCT CAGATACAGA GGACAGTGCT
GGAGGCGGGC CAGAGACCAG GGAAGCTCTG GCTGAGAGCG ACCGTGCAGC TGAGGGTCTG
GGGGCTGGAG TTGAGGAGGA AGGAGATGAT GGGAAGGAAC CCCAAGTTGG GGGCAGCCCC
CAACCCCTGA GCCATCCCAG CCCAGTGTGG ATGAACTACT CCTACAGCAG CCTGTGTTTG
AGCAGCGAGG AGTCAGAAAG CAGTGGGGAA GATGAGGAGT TCTGGGCTGA GCTGCAGAGT
CTTCGGCAGA AGCACTTGTC AGAGGTGGAA ACACTACAGA CACTACAGAA AAAAGAAATT
GAAGATTTGT ACAGCCGGCT GGGGAAGCAG CCCCCACCGG GTATTGTGGC CCCAGCTGCT
ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC AAGGGCAGCT TCCCCACCTC CCGCCGCAAC
AGCCTACAGC GCTCTGAGCC CCCAGGCCCT GTGCTCTCCT TCCCCCATCC TGCTCCCAGG
CATCATGCGA AGGAACTCTC TGAGTGGCAG CAGCACCGGC TCCCAGGAGC AGCGGTGAAT
TCAGAACAGA AGCCATGTAT CTCCCCCACA CCAGGGCCCA CCATGGAGCT TGTGTTCTCA
GAATCTGATG CTTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50594
Clone ID Related Accession (Same CDS sequence) XM_005257596
Accession Version XM_005257596.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product serine/threonine-protein kinase WNK4 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530412706. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGTTGGCAT CCCCGGCCAC GGAGACCACC GTCCTCATGT CCCAGACTGA GGCCGACCTG 
GCCCTGCGGC CCCCGCCTCC TCTTGGCACC GCGGGGCAGC CCCGCCTCGG GCCCCCTCCT
CGCCGAGCGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC TCGTCTCAGC
CGCCGTAGCT CAGTCGACTT GGGGCTGCTG AGCTCTTGGT CCCTGCCAGC CTCACCCGCT
CCGGACCCCC CCGATCCTCC GGACTCCGCT GGTCCTGGCC CCGCGAGGAG CCCACCGCCT
AGCTCCAAAG AACCCCCCGA GGGCACGTGG ACCGAGGGAG CCCCTGTGAA GGCTGCGGAA
GACTCCGCGC GTCCCGAGCT CCCGGACTCT GCAGTGGGCC CGGGGTCCAG GGAGCCGCTA
AGGGTCCCTG AAGCTGTGGC CCTAGAGCGG CGGCGGGAGC AGGAAGAAAA GGAGGACATG
GAGACCCAGG CTGTGGCAAC GTCCCCCGAT GGCCGATACC TCAAGTTTGA CATCGAGATT
GGACGTGGCT CCTTCAAGAC GGTGTATCGA GGGCTAGACA CCGACACCAC AGTGGAGGTG
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTAGAGCTG AGCGGCAGCG CTTCTCAGAG
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA TGATTCGTGG
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
ACGCTCAAGA CGTACCTGAG GCGGTTCCGG GAGATGAAGC CGCGGGTCCT TCAGCGCTGG
AGCCGCCAAA TCCTGCGGGG ACTTCATTTC CTACACTCCC GGGTTCCTCC CATCCTGCAC
CGGGATCTCA AGTGCGACAA TGTCTTTATC ACGGGACCTA CTGGCTCTGT CAAAATCGGG
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTTGCCA AGAGTGTCAT CGGGACCCCG
GAATTCATGG CCCCCGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
TTCGGCATGT GCATGCTGGA GATGGCCACC TCTGAGTACC CGTACTCCGA GTGCCAGAAT
GCCGCGCAAA TCTACCGCAA GGTCACTTCG GGCAGAAAGC CGAACAGCTT CCACAAGGTG
AAGATACCCG AGGTGAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAACGAGAGG
TTCACCATCC AGGACCTCCT GGCCCACGCC TTCTTCCGCG AGGAGCGCGG TGTGCACGTG
GAACTAGCGG AGGAGGACGA CGGCGAGAAG CCGGGCCTCA AGCTCTGGCT GCGCATGGAG
GACGCGCGGC GCGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCCT GTTCCAGCTG
GGCCGGGACG CGGCCGAGGA GGTGGCACAG GAGATGGTGG CTCTGGGCTT GGTCTGTGAA
GCCGATTACC AGCCAGTGGC CCGTGCAGTA CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
CGTGAGAAGC TGCGTAAAGC AAGGGAATTG GAGGCACTCC CACCAGAGCC AGGACCTCCA
CCAGCAACTG TGCCCATGGC CCCCGGTCCC CCCAGTGTCT TCCCCCCTGA GCCTGAGGAG
CCAGAGGCAG ACCAGCACCA GCCCTTCCTT TTCCGCCACG CCAGCTACTC ATCTACCACT
TCGGATTGCG AGACTGATGG CTACCTCAGC TCCTCCGGCT TCCTGGATGC CTCAGACCCT
GCCCTTCAGC CCCCTGGGGG GGTGCCATCC AGCCTGGCTG AGTCCCATCT CTGCCTGCCC
TCGGCTTTTG CCCTATCCAT TCCACGTTCT GGCCCTGGAA GTGACTTTTC CCCCGGGGAC
AGCTATGCCT CAGATGCAGC TTCAGGCCTT AGCGATGTGG GAGAAGGGAT GGGACAAATG
AGGAGACCCC CAGGGAGGAA TCTCCGGCGC AGACCCCGAT CCCGGCTGCG GGTCACTAGT
GTCTCAGACC AGAATGACAG AGTGGTTGAG TGCCAGCTAC AGACCCATAA CAGCAAGATG
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCGGAAG AGATTGCAGC TGCCATGGTA
TATAACGAGT TCATTCTGCC TTCGGAGCGA GATGGATTTC TCAGACGGAT TCGGGAGATT
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GACACTGGCC CCATGGAGGC TGCTGAAGAC
ACCCTAAGCC CCCAGGAGGA GCCAGCACCA TTACCTGCCC TGCCCGTCCC CCTCCCAGAC
CCATCCAATG AGCTCCAGAG CAGCACCTCC CTGGAGCACA GGAGCTGGAC AGCCTTCTCC
ACCTCCTCAT CTTCTCCTGG AACTCCTTTG TCTCCTGGAA ACCCATTTTC CCCTGGAACC
CCCATTTCCC CAGGTCCCAT CTTCCCCATC ACTTCTCCCC CATGTCATCC CAGCCCCTCC
CCATTCTCCC CCATTTCTTC CCAGGTCTCC TCAAATCCCT CTCCACACCC CACCAGCTCT
CCACTTCCAT TCTCCTCCAG CACACCCGAG TTTCCGGTCC CACTCTCTCA GTGTCCCTGG
AGTTCTCTCC CCACGACTTC TCCACCTACG TTCTCTCCCA CTTGTTCTCA GGTCACTCTT
AGTTCCCCTT TCTTTCCTCC GTGCCCCTCC ACTTCTTCCT TCCCCTCCAC CACAGCAGCC
CCTCTCCTTT CTCTGGCTAG TGCCTTCTCA CTGGCTGTGA TGACTGTGGC CCAGTCCCTG
CTGTCCCCCT CACCTGGGCT CCTTTCCCAG TCTCCTCCAG CCCCTCCTAG TCCCCTCCCT
AGCCTGCCCC TTCCCCCTCC CGTTGCTCCT GGTGGCCAGG AAAGCCCTTC ACCCCACACA
GCTGAGGTGG AGAGTGAGGC CTCACCACCT CCTGCTCGGC CCCTCCCAGG GGAAGCCAGG
CTGGCGCCCA TCTCTGAAGA GGGAAAGCCG CAGCTTGTTG GGCGTTTCCA AGTGACTTCA
TCCAAGGAAC CGGCTGAGCC TCTTCCCTTG CAGCCAACAT CCCCCACTCT CTCTGGTTCT
CCAAAACCTT CAACCCCTCA GCTCACTTCA GAGAGCTCAG ATACAGAGGA CAGTGCTGGA
GGCGGGCCAG AGACCAGGGA AGCTCTGGCT GAGAGCGACC GTGCAGCTGA GGGTCTGGGG
GCTGGAGTTG AGGAGGAAGG AGATGATGGG AAGGAACCCC AAGTTGGGGG CAGCCCCCAA
CCCCTGAGCC ATCCCAGCCC AGTGTGGATG AACTACTCCT ACAGCAGCCT GTGTTTGAGC
AGCGAGGAGT CAGAAAGCAG TGGGGAAGAT GAGGAGTTCT GGGCTGAGCT GCAGAGTCTT
CGGCAGAAGC ACTTGTCAGA GGTGGAAACA CTACAGACAC TACAGAAAAA AGAAATTGAA
GATTTGTACA GCCGGCTGGG GAAGCAGCCC CCACCGGGTA TTGTGGCCCC AGCTGCTATG
CTGTCCAGCC GCCAGCGCCG CCTCTCCAAG GGCAGCTTCC CCACCTCCCG CCGCAACAGC
CTACAGCGCT CTGAGCCCCC AGGCCCTGGC ATCATGCGAA GGAACTCTCT GAGTGGCAGC
AGCACCGGCT CCCAGGAGCA GCGGGCAAGC AAGGGGGTGA CATTCGCCGG GGATGTTGGC
AGGATGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005257653.1
CDS1027..4755
Misc Feature(1)1540..2322(+)
Misc Feature(2)1561..2322(+)
Misc Feature(3)1564..2049(+)
Misc Feature(4)1564..1989(+)
Misc Feature(5)1576..2049(+)
Misc Feature(6)1984..2049(+)
Misc Feature(7)2383..2493(+)
Translation

Target ORF information:

RefSeq Version XM_005257596
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005257596

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGTTGGCAT CCCCGGCCAC GGAGACCACC GTCCTCATGT CCCAGACTGA GGCCGACCTG 
GCCCTGCGGC CCCCGCCTCC TCTTGGCACC GCGGGGCAGC CCCGCCTCGG GCCCCCTCCT
CGCCGAGCGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC TCGTCTCAGC
CGCCGTAGCT CAGTCGACTT GGGGCTGCTG AGCTCTTGGT CCCTGCCAGC CTCACCCGCT
CCGGACCCCC CCGATCCTCC GGACTCCGCT GGTCCTGGCC CCGCGAGGAG CCCACCGCCT
AGCTCCAAAG AACCCCCCGA GGGCACGTGG ACCGAGGGAG CCCCTGTGAA GGCTGCGGAA
GACTCCGCGC GTCCCGAGCT CCCGGACTCT GCAGTGGGCC CGGGGTCCAG GGAGCCGCTA
AGGGTCCCTG AAGCTGTGGC CCTAGAGCGG CGGCGGGAGC AGGAAGAAAA GGAGGACATG
GAGACCCAGG CTGTGGCAAC GTCCCCCGAT GGCCGATACC TCAAGTTTGA CATCGAGATT
GGACGTGGCT CCTTCAAGAC GGTGTATCGA GGGCTAGACA CCGACACCAC AGTGGAGGTG
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTAGAGCTG AGCGGCAGCG CTTCTCAGAG
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA TGATTCGTGG
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
ACGCTCAAGA CGTACCTGAG GCGGTTCCGG GAGATGAAGC CGCGGGTCCT TCAGCGCTGG
AGCCGCCAAA TCCTGCGGGG ACTTCATTTC CTACACTCCC GGGTTCCTCC CATCCTGCAC
CGGGATCTCA AGTGCGACAA TGTCTTTATC ACGGGACCTA CTGGCTCTGT CAAAATCGGG
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTTGCCA AGAGTGTCAT CGGGACCCCG
GAATTCATGG CCCCCGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
TTCGGCATGT GCATGCTGGA GATGGCCACC TCTGAGTACC CGTACTCCGA GTGCCAGAAT
GCCGCGCAAA TCTACCGCAA GGTCACTTCG GGCAGAAAGC CGAACAGCTT CCACAAGGTG
AAGATACCCG AGGTGAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAACGAGAGG
TTCACCATCC AGGACCTCCT GGCCCACGCC TTCTTCCGCG AGGAGCGCGG TGTGCACGTG
GAACTAGCGG AGGAGGACGA CGGCGAGAAG CCGGGCCTCA AGCTCTGGCT GCGCATGGAG
GACGCGCGGC GCGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCCT GTTCCAGCTG
GGCCGGGACG CGGCCGAGGA GGTGGCACAG GAGATGGTGG CTCTGGGCTT GGTCTGTGAA
GCCGATTACC AGCCAGTGGC CCGTGCAGTA CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
CGTGAGAAGC TGCGTAAAGC AAGGGAATTG GAGGCACTCC CACCAGAGCC AGGACCTCCA
CCAGCAACTG TGCCCATGGC CCCCGGTCCC CCCAGTGTCT TCCCCCCTGA GCCTGAGGAG
CCAGAGGCAG ACCAGCACCA GCCCTTCCTT TTCCGCCACG CCAGCTACTC ATCTACCACT
TCGGATTGCG AGACTGATGG CTACCTCAGC TCCTCCGGCT TCCTGGATGC CTCAGACCCT
GCCCTTCAGC CCCCTGGGGG GGTGCCATCC AGCCTGGCTG AGTCCCATCT CTGCCTGCCC
TCGGCTTTTG CCCTATCCAT TCCACGTTCT GGCCCTGGAA GTGACTTTTC CCCCGGGGAC
AGCTATGCCT CAGATGCAGC TTCAGGCCTT AGCGATGTGG GAGAAGGGAT GGGACAAATG
AGGAGACCCC CAGGGAGGAA TCTCCGGCGC AGACCCCGAT CCCGGCTGCG GGTCACTAGT
GTCTCAGACC AGAATGACAG AGTGGTTGAG TGCCAGCTAC AGACCCATAA CAGCAAGATG
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCGGAAG AGATTGCAGC TGCCATGGTA
TATAACGAGT TCATTCTGCC TTCGGAGCGA GATGGATTTC TCAGACGGAT TCGGGAGATT
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GACACTGGCC CCATGGAGGC TGCTGAAGAC
ACCCTAAGCC CCCAGGAGGA GCCAGCACCA TTACCTGCCC TGCCCGTCCC CCTCCCAGAC
CCATCCAATG AGCTCCAGAG CAGCACCTCC CTGGAGCACA GGAGCTGGAC AGCCTTCTCC
ACCTCCTCAT CTTCTCCTGG AACTCCTTTG TCTCCTGGAA ACCCATTTTC CCCTGGAACC
CCCATTTCCC CAGGTCCCAT CTTCCCCATC ACTTCTCCCC CATGTCATCC CAGCCCCTCC
CCATTCTCCC CCATTTCTTC CCAGGTCTCC TCAAATCCCT CTCCACACCC CACCAGCTCT
CCACTTCCAT TCTCCTCCAG CACACCCGAG TTTCCGGTCC CACTCTCTCA GTGTCCCTGG
AGTTCTCTCC CCACGACTTC TCCACCTACG TTCTCTCCCA CTTGTTCTCA GGTCACTCTT
AGTTCCCCTT TCTTTCCTCC GTGCCCCTCC ACTTCTTCCT TCCCCTCCAC CACAGCAGCC
CCTCTCCTTT CTCTGGCTAG TGCCTTCTCA CTGGCTGTGA TGACTGTGGC CCAGTCCCTG
CTGTCCCCCT CACCTGGGCT CCTTTCCCAG TCTCCTCCAG CCCCTCCTAG TCCCCTCCCT
AGCCTGCCCC TTCCCCCTCC CGTTGCTCCT GGTGGCCAGG AAAGCCCTTC ACCCCACACA
GCTGAGGTGG AGAGTGAGGC CTCACCACCT CCTGCTCGGC CCCTCCCAGG GGAAGCCAGG
CTGGCGCCCA TCTCTGAAGA GGGAAAGCCG CAGCTTGTTG GGCGTTTCCA AGTGACTTCA
TCCAAGGAAC CGGCTGAGCC TCTTCCCTTG CAGCCAACAT CCCCCACTCT CTCTGGTTCT
CCAAAACCTT CAACCCCTCA GCTCACTTCA GAGAGCTCAG ATACAGAGGA CAGTGCTGGA
GGCGGGCCAG AGACCAGGGA AGCTCTGGCT GAGAGCGACC GTGCAGCTGA GGGTCTGGGG
GCTGGAGTTG AGGAGGAAGG AGATGATGGG AAGGAACCCC AAGTTGGGGG CAGCCCCCAA
CCCCTGAGCC ATCCCAGCCC AGTGTGGATG AACTACTCCT ACAGCAGCCT GTGTTTGAGC
AGCGAGGAGT CAGAAAGCAG TGGGGAAGAT GAGGAGTTCT GGGCTGAGCT GCAGAGTCTT
CGGCAGAAGC ACTTGTCAGA GGTGGAAACA CTACAGACAC TACAGAAAAA AGAAATTGAA
GATTTGTACA GCCGGCTGGG GAAGCAGCCC CCACCGGGTA TTGTGGCCCC AGCTGCTATG
CTGTCCAGCC GCCAGCGCCG CCTCTCCAAG GGCAGCTTCC CCACCTCCCG CCGCAACAGC
CTACAGCGCT CTGAGCCCCC AGGCCCTGGC ATCATGCGAA GGAACTCTCT GAGTGGCAGC
AGCACCGGCT CCCAGGAGCA GCGGGCAAGC AAGGGGGTGA CATTCGCCGG GGATGTTGGC
AGGATGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50595
Clone ID Related Accession (Same CDS sequence) XM_005257597
Accession Version XM_005257597.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3714bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product serine/threonine-protein kinase WNK4 isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578831264. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGTTGGCAT CCCCGGCCAC GGAGACCACC GTCCTCATGT CCCAGACTGA GGCCGACCTG 
GCCCTGCGGC CCCCGCCTCC TCTTGGCACC GCGGGGCAGC CCCGCCTCGG GCCCCCTCCT
CGCCGAGCGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC TCGTCTCAGC
CGCCGTAGCT CAGTCGACTT GGGGCTGCTG AGCTCTTGGT CCCTGCCAGC CTCACCCGCT
CCGGACCCCC CCGATCCTCC GGACTCCGCT GGTCCTGGCC CCGCGAGGAG CCCACCGCCT
AGCTCCAAAG AACCCCCCGA GGGCACGTGG ACCGAGGGAG CCCCTGTGAA GGCTGCGGAA
GACTCCGCGC GTCCCGAGCT CCCGGACTCT GCAGTGGGCC CGGGGTCCAG GGAGCCGCTA
AGGGTCCCTG AAGCTGTGGC CCTAGAGCGG CGGCGGGAGC AGGAAGAAAA GGAGGACATG
GAGACCCAGG CTGTGGCAAC GTCCCCCGAT GGCCGATACC TCAAGTTTGA CATCGAGATT
GGACGTGGCT CCTTCAAGAC GGTGTATCGA GGGCTAGACA CCGACACCAC AGTGGAGGTG
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTAGAGCTG AGCGGCAGCG CTTCTCAGAG
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA TGATTCGTGG
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
ACGCTCAAGA CGTACCTGAG GCGGTTCCGG GAGATGAAGC CGCGGGTCCT TCAGCGCTGG
AGCCGCCAAA TCCTGCGGGG ACTTCATTTC CTACACTCCC GGGTTCCTCC CATCCTGCAC
CGGGATCTCA AGTGCGACAA TGTCTTTATC ACGGGACCTA CTGGCTCTGT CAAAATCGGG
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTTGCCA AGAGTGTCAT CGGGACCCCG
GAATTCATGG CCCCCGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
TTCGGCATGT GCATGCTGGA GATGGCCACC TCTGAGTACC CGTACTCCGA GTGCCAGAAT
GCCGCGCAAA TCTACCGCAA GGTCACTTCG GGCAGAAAGC CGAACAGCTT CCACAAGGTG
AAGATACCCG AGGTGAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAACGAGAGG
TTCACCATCC AGGACCTCCT GGCCCACGCC TTCTTCCGCG AGGAGCGCGG TGTGCACGTG
GAACTAGCGG AGGAGGACGA CGGCGAGAAG CCGGGCCTCA AGCTCTGGCT GCGCATGGAG
GACGCGCGGC GCGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCCT GTTCCAGCTG
GGCCGGGACG CGGCCGAGGA GGTGGCACAG GAGATGGTGG CTCTGGGCTT GGTCTGTGAA
GCCGATTACC AGCCAGTGGC CCGTGCAGTA CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
CGTGAGAAGC TGCGTAAAGC AAGGGAATTG GAGGCACTCC CACCAGAGCC AGGACCTCCA
CCAGCAACTG TGCCCATGGC CCCCGGTCCC CCCAGTGTCT TCCCCCCTGA GCCTGAGGAG
CCAGAGGCAG ACCAGCACCA GCCCTTCCTT TTCCGCCACG CCAGCTACTC ATCTACCACT
TCGGATTGCG AGACTGATGG CTACCTCAGC TCCTCCGGCT TCCTGGATGC CTCAGACCCT
GCCCTTCAGC CCCCTGGGGG GGTGCCATCC AGCCTGGCTG AGTCCCATCT CTGCCTGCCC
TCGGCTTTTG CCCTATCCAT TCCACGTTCT GGCCCTGGAA GTGACTTTTC CCCCGGGGAC
AGCTATGCCT CAGATGCAGC TTCAGGCCTT AGCGATGTGG GAGAAGGGAT GGGACAAATG
AGGAGACCCC CAGGGAGGAA TCTCCGGCGC AGACCCCGAT CCCGGCTGCG GGTCACTAGT
GTCTCAGACC AGAATGACAG AGTGGTTGAG TGCCAGCTAC AGACCCATAA CAGCAAGATG
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCGGAAG AGATTGCAGC TGCCATGGTA
TATAACGAGT TCATTCTGCC TTCGGAGCGA GATGGATTTC TCAGACGGAT TCGGGAGATT
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GACACTGGCC CCATGGAGGC TGCTGAAGAC
ACCCTAAGCC CCCAGGAGGA GCCAGCACCA TTACCTGCCC TGCCCGTCCC CCTCCCAGAC
CCATCCAATG AAGAGCTCCA GAGCAGCACC TCCCTGGAGC ACAGGAGCTG GACAGCCTTC
TCCACCTCCT CATCTTCTCC TGGAACTCCT TTGTCTCCTG GAAACCCATT TTCCCCTGGA
ACCCCCATTT CCCCAGGTCC CATCTTCCCC ATCACTTCTC CCCCATGTCA TCCCAGCCCC
TCCCCATTCT CCCCCATTTC TTCCCAGGTC TCCTCAAATC CCTCTCCACA CCCCACCAGC
TCTCCACTTC CATTCTCCTC CAGCACACCC GAGTTTCCGG TCCCACTCTC TCAGTGTCCC
TGGAGTTCTC TCCCCACGAC TTCTCCACCT ACGTTCTCTC CCACTTGTTC TCAGGTCACT
CTTAGTTCCC CTTTCTTTCC TCCGTGCCCC TCCACTTCTT CCTTCCCCTC CACCACAGCA
GCCCCTCTCC TTTCTCTGGC TAGTGCCTTC TCACTGGCTG TGATGACTGT GGCCCAGTCC
CTGCTGTCCC CCTCACCTGG GCTCCTTTCC CAGTCTCCTC CAGCCCCTCC TAGTCCCCTC
CCTAGCCTGC CCCTTCCCCC TCCCGTTGCT CCTGGTGGCC AGGAAAGCCC TTCACCCCAC
ACAGCTGAGG TGGAGAGTGA GGCCTCACCA CCTCCTGCTC GGCCCCTCCC AGGGGAAGCC
AGGCTGGCGC CCATCTCTGA AGAGGGAAAG CCGCAGCTTG TTGGGCGTTT CCAAGTGACT
TCATCCAAGG AACCGGCTGA GCCTCTTCCC TTGCAGCCAA CATCCCCCAC TCTCTCTGGT
TCTCCAAAAC CTTCAACCCC TCAGCTCACT TCAGAGAGCT CAGATACAGA GGACAGTGCT
GGAGGCGGGC CAGAGACCAG GGAAGCTCTG GCTGAGAGCG ACCGTGCAGC TGAGGGTCTG
GGGGCTGGAG TTGAGGAGGA AGGAGATGAT GGGAAGGAAC CCCAAGTTGG GGGCAGCCCC
CAACCCCTGA GCCATCCCAG CCCAGTGTGG ATGAACTACT CCTACAGCAG CCTGTGTTTG
AGCAGCGAGG AGTCAGAAAG CAGTGGGGAA GATGAGGAGT TCTGGGCTGA GCTGCAGAGT
CTTCGGCAGA AGCACTTGTC AGAGGTGGAA ACACTACAGA CACTACAGAA AAAAGAAATT
GAAGATTTGT ACAGCCGGCT GGGGAAGCAG CCCCCACCGG GTATTGTGGC CCCAGCTGCT
ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC AAGGGCAGCT TCCCCACCTC CCGCCGCAAC
AGCCTACAGC GCTCTGAGCC CCCAGGCCCT GTGAATTCAG AACAGAAGCC ATGTATCTCC
CCCACACCAG GGCCCACCAT GGAGCTTGTG TTCTCAGAAT CTGATGCTTT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005257654.1
CDS1027..4740
Misc Feature(1)1540..2322(+)
Misc Feature(2)1561..2322(+)
Misc Feature(3)1564..2049(+)
Misc Feature(4)1564..1989(+)
Misc Feature(5)1576..2049(+)
Misc Feature(6)1984..2049(+)
Misc Feature(7)2383..2493(+)
Translation

Target ORF information:

RefSeq Version XM_005257597
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005257597

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGTTGGCAT CCCCGGCCAC GGAGACCACC GTCCTCATGT CCCAGACTGA GGCCGACCTG 
GCCCTGCGGC CCCCGCCTCC TCTTGGCACC GCGGGGCAGC CCCGCCTCGG GCCCCCTCCT
CGCCGAGCGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC TCGTCTCAGC
CGCCGTAGCT CAGTCGACTT GGGGCTGCTG AGCTCTTGGT CCCTGCCAGC CTCACCCGCT
CCGGACCCCC CCGATCCTCC GGACTCCGCT GGTCCTGGCC CCGCGAGGAG CCCACCGCCT
AGCTCCAAAG AACCCCCCGA GGGCACGTGG ACCGAGGGAG CCCCTGTGAA GGCTGCGGAA
GACTCCGCGC GTCCCGAGCT CCCGGACTCT GCAGTGGGCC CGGGGTCCAG GGAGCCGCTA
AGGGTCCCTG AAGCTGTGGC CCTAGAGCGG CGGCGGGAGC AGGAAGAAAA GGAGGACATG
GAGACCCAGG CTGTGGCAAC GTCCCCCGAT GGCCGATACC TCAAGTTTGA CATCGAGATT
GGACGTGGCT CCTTCAAGAC GGTGTATCGA GGGCTAGACA CCGACACCAC AGTGGAGGTG
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTAGAGCTG AGCGGCAGCG CTTCTCAGAG
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA TGATTCGTGG
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
ACGCTCAAGA CGTACCTGAG GCGGTTCCGG GAGATGAAGC CGCGGGTCCT TCAGCGCTGG
AGCCGCCAAA TCCTGCGGGG ACTTCATTTC CTACACTCCC GGGTTCCTCC CATCCTGCAC
CGGGATCTCA AGTGCGACAA TGTCTTTATC ACGGGACCTA CTGGCTCTGT CAAAATCGGG
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTTGCCA AGAGTGTCAT CGGGACCCCG
GAATTCATGG CCCCCGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
TTCGGCATGT GCATGCTGGA GATGGCCACC TCTGAGTACC CGTACTCCGA GTGCCAGAAT
GCCGCGCAAA TCTACCGCAA GGTCACTTCG GGCAGAAAGC CGAACAGCTT CCACAAGGTG
AAGATACCCG AGGTGAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAACGAGAGG
TTCACCATCC AGGACCTCCT GGCCCACGCC TTCTTCCGCG AGGAGCGCGG TGTGCACGTG
GAACTAGCGG AGGAGGACGA CGGCGAGAAG CCGGGCCTCA AGCTCTGGCT GCGCATGGAG
GACGCGCGGC GCGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCCT GTTCCAGCTG
GGCCGGGACG CGGCCGAGGA GGTGGCACAG GAGATGGTGG CTCTGGGCTT GGTCTGTGAA
GCCGATTACC AGCCAGTGGC CCGTGCAGTA CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
CGTGAGAAGC TGCGTAAAGC AAGGGAATTG GAGGCACTCC CACCAGAGCC AGGACCTCCA
CCAGCAACTG TGCCCATGGC CCCCGGTCCC CCCAGTGTCT TCCCCCCTGA GCCTGAGGAG
CCAGAGGCAG ACCAGCACCA GCCCTTCCTT TTCCGCCACG CCAGCTACTC ATCTACCACT
TCGGATTGCG AGACTGATGG CTACCTCAGC TCCTCCGGCT TCCTGGATGC CTCAGACCCT
GCCCTTCAGC CCCCTGGGGG GGTGCCATCC AGCCTGGCTG AGTCCCATCT CTGCCTGCCC
TCGGCTTTTG CCCTATCCAT TCCACGTTCT GGCCCTGGAA GTGACTTTTC CCCCGGGGAC
AGCTATGCCT CAGATGCAGC TTCAGGCCTT AGCGATGTGG GAGAAGGGAT GGGACAAATG
AGGAGACCCC CAGGGAGGAA TCTCCGGCGC AGACCCCGAT CCCGGCTGCG GGTCACTAGT
GTCTCAGACC AGAATGACAG AGTGGTTGAG TGCCAGCTAC AGACCCATAA CAGCAAGATG
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCGGAAG AGATTGCAGC TGCCATGGTA
TATAACGAGT TCATTCTGCC TTCGGAGCGA GATGGATTTC TCAGACGGAT TCGGGAGATT
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GACACTGGCC CCATGGAGGC TGCTGAAGAC
ACCCTAAGCC CCCAGGAGGA GCCAGCACCA TTACCTGCCC TGCCCGTCCC CCTCCCAGAC
CCATCCAATG AAGAGCTCCA GAGCAGCACC TCCCTGGAGC ACAGGAGCTG GACAGCCTTC
TCCACCTCCT CATCTTCTCC TGGAACTCCT TTGTCTCCTG GAAACCCATT TTCCCCTGGA
ACCCCCATTT CCCCAGGTCC CATCTTCCCC ATCACTTCTC CCCCATGTCA TCCCAGCCCC
TCCCCATTCT CCCCCATTTC TTCCCAGGTC TCCTCAAATC CCTCTCCACA CCCCACCAGC
TCTCCACTTC CATTCTCCTC CAGCACACCC GAGTTTCCGG TCCCACTCTC TCAGTGTCCC
TGGAGTTCTC TCCCCACGAC TTCTCCACCT ACGTTCTCTC CCACTTGTTC TCAGGTCACT
CTTAGTTCCC CTTTCTTTCC TCCGTGCCCC TCCACTTCTT CCTTCCCCTC CACCACAGCA
GCCCCTCTCC TTTCTCTGGC TAGTGCCTTC TCACTGGCTG TGATGACTGT GGCCCAGTCC
CTGCTGTCCC CCTCACCTGG GCTCCTTTCC CAGTCTCCTC CAGCCCCTCC TAGTCCCCTC
CCTAGCCTGC CCCTTCCCCC TCCCGTTGCT CCTGGTGGCC AGGAAAGCCC TTCACCCCAC
ACAGCTGAGG TGGAGAGTGA GGCCTCACCA CCTCCTGCTC GGCCCCTCCC AGGGGAAGCC
AGGCTGGCGC CCATCTCTGA AGAGGGAAAG CCGCAGCTTG TTGGGCGTTT CCAAGTGACT
TCATCCAAGG AACCGGCTGA GCCTCTTCCC TTGCAGCCAA CATCCCCCAC TCTCTCTGGT
TCTCCAAAAC CTTCAACCCC TCAGCTCACT TCAGAGAGCT CAGATACAGA GGACAGTGCT
GGAGGCGGGC CAGAGACCAG GGAAGCTCTG GCTGAGAGCG ACCGTGCAGC TGAGGGTCTG
GGGGCTGGAG TTGAGGAGGA AGGAGATGAT GGGAAGGAAC CCCAAGTTGG GGGCAGCCCC
CAACCCCTGA GCCATCCCAG CCCAGTGTGG ATGAACTACT CCTACAGCAG CCTGTGTTTG
AGCAGCGAGG AGTCAGAAAG CAGTGGGGAA GATGAGGAGT TCTGGGCTGA GCTGCAGAGT
CTTCGGCAGA AGCACTTGTC AGAGGTGGAA ACACTACAGA CACTACAGAA AAAAGAAATT
GAAGATTTGT ACAGCCGGCT GGGGAAGCAG CCCCCACCGG GTATTGTGGC CCCAGCTGCT
ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC AAGGGCAGCT TCCCCACCTC CCGCCGCAAC
AGCCTACAGC GCTCTGAGCC CCCAGGCCCT GTGAATTCAG AACAGAAGCC ATGTATCTCC
CCCACACCAG GGCCCACCAT GGAGCTTGTG TTCTCAGAAT CTGATGCTTT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50596
Clone ID Related Accession (Same CDS sequence) XM_006722020
Accession Version XM_006722020.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3702bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product serine/threonine-protein kinase WNK4 isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578831265. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGTTGGCAT CCCCGGCCAC GGAGACCACC GTCCTCATGT CCCAGACTGA GGCCGACCTG 
GCCCTGCGGC CCCCGCCTCC TCTTGGCACC GCGGGGCAGC CCCGCCTCGG GCCCCCTCCT
CGCCGAGCGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC TCGTCTCAGC
CGCCGTAGCT CAGTCGACTT GGGGCTGCTG AGCTCTTGGT CCCTGCCAGC CTCACCCGCT
CCGGACCCCC CCGATCCTCC GGACTCCGCT GGTCCTGGCC CCGCGAGGAG CCCACCGCCT
AGCTCCAAAG AACCCCCCGA GGGCACGTGG ACCGAGGGAG CCCCTGTGAA GGCTGCGGAA
GACTCCGCGC GTCCCGAGCT CCCGGACTCT GCAGTGGGCC CGGGGTCCAG GGAGCCGCTA
AGGGTCCCTG AAGCTGTGGC CCTAGAGCGG CGGCGGGAGC AGGAAGAAAA GGAGGACATG
GAGACCCAGG CTGTGGCAAC GTCCCCCGAT GGCCGATACC TCAAGTTTGA CATCGAGATT
GGACGTGGCT CCTTCAAGAC GGTGTATCGA GGGCTAGACA CCGACACCAC AGTGGAGGTG
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTAGAGCTG AGCGGCAGCG CTTCTCAGAG
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA TGATTCGTGG
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
ACGCTCAAGA CGTACCTGAG GCGGTTCCGG GAGATGAAGC CGCGGGTCCT TCAGCGCTGG
AGCCGCCAAA TCCTGCGGGG ACTTCATTTC CTACACTCCC GGGTTCCTCC CATCCTGCAC
CGGGATCTCA AGTGCGACAA TGTCTTTATC ACGGGACCTA CTGGCTCTGT CAAAATCGGG
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTTGCCA AGAGTGTCAT CGGGACCCCG
GAATTCATGG CCCCCGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
TTCGGCATGT GCATGCTGGA GATGGCCACC TCTGAGTACC CGTACTCCGA GTGCCAGAAT
GCCGCGCAAA TCTACCGCAA GGTCACTTCG GGCAGAAAGC CGAACAGCTT CCACAAGGTG
AAGATACCCG AGGTGAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAACGAGAGG
TTCACCATCC AGGACCTCCT GGCCCACGCC TTCTTCCGCG AGGAGCGCGG TGTGCACGTG
GAACTAGCGG AGGAGGACGA CGGCGAGAAG CCGGGCCTCA AGCTCTGGCT GCGCATGGAG
GACGCGCGGC GCGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCCT GTTCCAGCTG
GGCCGGGACG CGGCCGAGGA GGTGGCACAG GAGATGGTGG CTCTGGGCTT GGTCTGTGAA
GCCGATTACC AGCCAGTGGC CCGTGCAGTA CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
CGTGAGAAGC TGCGTAAAGC AAGGGAATTG GAGGCACTCC CACCAGAGCC AGGACCTCCA
CCAGCAACTG TGCCCATGGC CCCCGGTCCC CCCAGTGTCT TCCCCCCTGA GCCTGAGGAG
CCAGAGGCAG ACCAGCACCA GCCCTTCCTT TTCCGCCACG CCAGCTACTC ATCTACCACT
TCGGATTGCG AGACTGATGG CTACCTCAGC TCCTCCGGCT TCCTGGATGC CTCAGACCCT
GCCCTTCAGC CCCCTGGGGG GGTGCCATCC AGCCTGGCTG AGTCCCATCT CTGCCTGCCC
TCGGCTTTTG CCCTATCCAT TCCACGTTCT GGCCCTGGAA GTGACTTTTC CCCCGGGGAC
AGCTATGCCT CAGATGCAGC TTCAGGCCTT AGCGATGTGG GAGAAGGGAT GGGACAAATG
AGGAGACCCC CAGGGAGGAA TCTCCGGCGC AGACCCCGAT CCCGGCTGCG GGTCACTAGT
GTCTCAGACC AGAATGACAG AGTGGTTGAG TGCCAGCTAC AGACCCATAA CAGCAAGATG
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCGGAAG AGATTGCAGC TGCCATGGAG
GAGCCAGCAC CATTACCTGC CCTGCCCGTC CCCCTCCCAG ACCCATCCAA TGAAGAGCTC
CAGAGCAGCA CCTCCCTGGA GCACAGGAGC TGGACAGCCT TCTCCACCTC CTCATCTTCT
CCTGGAACTC CTTTGTCTCC TGGAAACCCA TTTTCCCCTG GAACCCCCAT TTCCCCAGGT
CCCATCTTCC CCATCACTTC TCCCCCATGT CATCCCAGCC CCTCCCCATT CTCCCCCATT
TCTTCCCAGG TCTCCTCAAA TCCCTCTCCA CACCCCACCA GCTCTCCACT TCCATTCTCC
TCCAGCACAC CCGAGTTTCC GGTCCCACTC TCTCAGTGTC CCTGGAGTTC TCTCCCCACG
ACTTCTCCAC CTACGTTCTC TCCCACTTGT TCTCAGGTCA CTCTTAGTTC CCCTTTCTTT
CCTCCGTGCC CCTCCACTTC TTCCTTCCCC TCCACCACAG CAGCCCCTCT CCTTTCTCTG
GCTAGTGCCT TCTCACTGGC TGTGATGACT GTGGCCCAGT CCCTGCTGTC CCCCTCACCT
GGGCTCCTTT CCCAGTCTCC TCCAGCCCCT CCTAGTCCCC TCCCTAGCCT GCCCCTTCCC
CCTCCCGTTG CTCCTGGTGG CCAGGAAAGC CCTTCACCCC ACACAGCTGA GGTGGAGAGT
GAGGCCTCAC CACCTCCTGC TCGGCCCCTC CCAGGGGAAG CCAGGCTGGC GCCCATCTCT
GAAGAGGGAA AGCCGCAGCT TGTTGGGCGT TTCCAAGTGA CTTCATCCAA GGAACCGGCT
GAGCCTCTTC CCTTGCAGCC AACATCCCCC ACTCTCTCTG GTTCTCCAAA ACCTTCAACC
CCTCAGCTCA CTTCAGAGAG CTCAGATACA GAGGACAGTG CTGGAGGCGG GCCAGAGACC
AGGGAAGCTC TGGCTGAGAG CGACCGTGCA GCTGAGGGTC TGGGGGCTGG AGTTGAGGAG
GAAGGAGATG ATGGGAAGGA ACCCCAAGTT GGGGGCAGCC CCCAACCCCT GAGCCATCCC
AGCCCAGTGT GGATGAACTA CTCCTACAGC AGCCTGTGTT TGAGCAGCGA GGAGTCAGAA
AGCAGTGGGG AAGATGAGGA GTTCTGGGCT GAGCTGCAGA GTCTTCGGCA GAAGCACTTG
TCAGAGGTGG AAACACTACA GACACTACAG AAAAAAGAAA TTGAAGATTT GTACAGCCGG
CTGGGGAAGC AGCCCCCACC GGGTATTGTG GCCCCAGCTG CTATGCTGTC CAGCCGCCAG
CGCCGCCTCT CCAAGGGCAG CTTCCCCACC TCCCGCCGCA ACAGCCTACA GCGCTCTGAG
CCCCCAGGCC CTGTGCTCTC CTTCCCCCAT CCTGCTCCCA GGCATCATGC GAAGGAACTC
TCTGAGTGGC AGCAGCACCG GCTCCCAGGA GCAGCGGGCA AGCAAGGGGG TGACATTCGC
CGGGGATGTT GGCAGGATGT GAATTCAGAA CAGAAGCCAT GTATCTCCCC CACACCAGGG
CCCACCATGG AGCTTGTGTT CTCAGAATCT GATGCTTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006722083.1
CDS1027..4728
Misc Feature(1)1540..2322(+)
Misc Feature(2)1561..2322(+)
Misc Feature(3)1564..2049(+)
Misc Feature(4)1564..1989(+)
Misc Feature(5)1576..2049(+)
Misc Feature(6)1984..2049(+)
Misc Feature(7)2383..2493(+)
Translation

Target ORF information:

RefSeq Version XM_006722020
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006722020

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGTTGGCAT CCCCGGCCAC GGAGACCACC GTCCTCATGT CCCAGACTGA GGCCGACCTG 
GCCCTGCGGC CCCCGCCTCC TCTTGGCACC GCGGGGCAGC CCCGCCTCGG GCCCCCTCCT
CGCCGAGCGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC TCGTCTCAGC
CGCCGTAGCT CAGTCGACTT GGGGCTGCTG AGCTCTTGGT CCCTGCCAGC CTCACCCGCT
CCGGACCCCC CCGATCCTCC GGACTCCGCT GGTCCTGGCC CCGCGAGGAG CCCACCGCCT
AGCTCCAAAG AACCCCCCGA GGGCACGTGG ACCGAGGGAG CCCCTGTGAA GGCTGCGGAA
GACTCCGCGC GTCCCGAGCT CCCGGACTCT GCAGTGGGCC CGGGGTCCAG GGAGCCGCTA
AGGGTCCCTG AAGCTGTGGC CCTAGAGCGG CGGCGGGAGC AGGAAGAAAA GGAGGACATG
GAGACCCAGG CTGTGGCAAC GTCCCCCGAT GGCCGATACC TCAAGTTTGA CATCGAGATT
GGACGTGGCT CCTTCAAGAC GGTGTATCGA GGGCTAGACA CCGACACCAC AGTGGAGGTG
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTAGAGCTG AGCGGCAGCG CTTCTCAGAG
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA TGATTCGTGG
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
ACGCTCAAGA CGTACCTGAG GCGGTTCCGG GAGATGAAGC CGCGGGTCCT TCAGCGCTGG
AGCCGCCAAA TCCTGCGGGG ACTTCATTTC CTACACTCCC GGGTTCCTCC CATCCTGCAC
CGGGATCTCA AGTGCGACAA TGTCTTTATC ACGGGACCTA CTGGCTCTGT CAAAATCGGG
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTTGCCA AGAGTGTCAT CGGGACCCCG
GAATTCATGG CCCCCGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
TTCGGCATGT GCATGCTGGA GATGGCCACC TCTGAGTACC CGTACTCCGA GTGCCAGAAT
GCCGCGCAAA TCTACCGCAA GGTCACTTCG GGCAGAAAGC CGAACAGCTT CCACAAGGTG
AAGATACCCG AGGTGAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAACGAGAGG
TTCACCATCC AGGACCTCCT GGCCCACGCC TTCTTCCGCG AGGAGCGCGG TGTGCACGTG
GAACTAGCGG AGGAGGACGA CGGCGAGAAG CCGGGCCTCA AGCTCTGGCT GCGCATGGAG
GACGCGCGGC GCGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCCT GTTCCAGCTG
GGCCGGGACG CGGCCGAGGA GGTGGCACAG GAGATGGTGG CTCTGGGCTT GGTCTGTGAA
GCCGATTACC AGCCAGTGGC CCGTGCAGTA CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
CGTGAGAAGC TGCGTAAAGC AAGGGAATTG GAGGCACTCC CACCAGAGCC AGGACCTCCA
CCAGCAACTG TGCCCATGGC CCCCGGTCCC CCCAGTGTCT TCCCCCCTGA GCCTGAGGAG
CCAGAGGCAG ACCAGCACCA GCCCTTCCTT TTCCGCCACG CCAGCTACTC ATCTACCACT
TCGGATTGCG AGACTGATGG CTACCTCAGC TCCTCCGGCT TCCTGGATGC CTCAGACCCT
GCCCTTCAGC CCCCTGGGGG GGTGCCATCC AGCCTGGCTG AGTCCCATCT CTGCCTGCCC
TCGGCTTTTG CCCTATCCAT TCCACGTTCT GGCCCTGGAA GTGACTTTTC CCCCGGGGAC
AGCTATGCCT CAGATGCAGC TTCAGGCCTT AGCGATGTGG GAGAAGGGAT GGGACAAATG
AGGAGACCCC CAGGGAGGAA TCTCCGGCGC AGACCCCGAT CCCGGCTGCG GGTCACTAGT
GTCTCAGACC AGAATGACAG AGTGGTTGAG TGCCAGCTAC AGACCCATAA CAGCAAGATG
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCGGAAG AGATTGCAGC TGCCATGGAG
GAGCCAGCAC CATTACCTGC CCTGCCCGTC CCCCTCCCAG ACCCATCCAA TGAAGAGCTC
CAGAGCAGCA CCTCCCTGGA GCACAGGAGC TGGACAGCCT TCTCCACCTC CTCATCTTCT
CCTGGAACTC CTTTGTCTCC TGGAAACCCA TTTTCCCCTG GAACCCCCAT TTCCCCAGGT
CCCATCTTCC CCATCACTTC TCCCCCATGT CATCCCAGCC CCTCCCCATT CTCCCCCATT
TCTTCCCAGG TCTCCTCAAA TCCCTCTCCA CACCCCACCA GCTCTCCACT TCCATTCTCC
TCCAGCACAC CCGAGTTTCC GGTCCCACTC TCTCAGTGTC CCTGGAGTTC TCTCCCCACG
ACTTCTCCAC CTACGTTCTC TCCCACTTGT TCTCAGGTCA CTCTTAGTTC CCCTTTCTTT
CCTCCGTGCC CCTCCACTTC TTCCTTCCCC TCCACCACAG CAGCCCCTCT CCTTTCTCTG
GCTAGTGCCT TCTCACTGGC TGTGATGACT GTGGCCCAGT CCCTGCTGTC CCCCTCACCT
GGGCTCCTTT CCCAGTCTCC TCCAGCCCCT CCTAGTCCCC TCCCTAGCCT GCCCCTTCCC
CCTCCCGTTG CTCCTGGTGG CCAGGAAAGC CCTTCACCCC ACACAGCTGA GGTGGAGAGT
GAGGCCTCAC CACCTCCTGC TCGGCCCCTC CCAGGGGAAG CCAGGCTGGC GCCCATCTCT
GAAGAGGGAA AGCCGCAGCT TGTTGGGCGT TTCCAAGTGA CTTCATCCAA GGAACCGGCT
GAGCCTCTTC CCTTGCAGCC AACATCCCCC ACTCTCTCTG GTTCTCCAAA ACCTTCAACC
CCTCAGCTCA CTTCAGAGAG CTCAGATACA GAGGACAGTG CTGGAGGCGG GCCAGAGACC
AGGGAAGCTC TGGCTGAGAG CGACCGTGCA GCTGAGGGTC TGGGGGCTGG AGTTGAGGAG
GAAGGAGATG ATGGGAAGGA ACCCCAAGTT GGGGGCAGCC CCCAACCCCT GAGCCATCCC
AGCCCAGTGT GGATGAACTA CTCCTACAGC AGCCTGTGTT TGAGCAGCGA GGAGTCAGAA
AGCAGTGGGG AAGATGAGGA GTTCTGGGCT GAGCTGCAGA GTCTTCGGCA GAAGCACTTG
TCAGAGGTGG AAACACTACA GACACTACAG AAAAAAGAAA TTGAAGATTT GTACAGCCGG
CTGGGGAAGC AGCCCCCACC GGGTATTGTG GCCCCAGCTG CTATGCTGTC CAGCCGCCAG
CGCCGCCTCT CCAAGGGCAG CTTCCCCACC TCCCGCCGCA ACAGCCTACA GCGCTCTGAG
CCCCCAGGCC CTGTGCTCTC CTTCCCCCAT CCTGCTCCCA GGCATCATGC GAAGGAACTC
TCTGAGTGGC AGCAGCACCG GCTCCCAGGA GCAGCGGGCA AGCAAGGGGG TGACATTCGC
CGGGGATGTT GGCAGGATGT GAATTCAGAA CAGAAGCCAT GTATCTCCCC CACACCAGGG
CCCACCATGG AGCTTGTGTT CTCAGAATCT GATGCTTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71643
Clone ID Related Accession (Same CDS sequence) XM_011525134
Accession Version XM_011525134.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3699bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product serine/threonine-protein kinase WNK4 isoform X7
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGTTGGCAT CCCCGGCCAC GGAGACCACC GTCCTCATGT CCCAGACTGA GGCCGACCTG 
GCCCTGCGGC CCCCGCCTCC TCTTGGCACC GCGGGGCAGC CCCGCCTCGG GCCCCCTCCT
CGCCGAGCGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC TCGTCTCAGC
CGCCGTAGCT CAGTCGACTT GGGGCTGCTG AGCTCTTGGT CCCTGCCAGC CTCACCCGCT
CCGGACCCCC CCGATCCTCC GGACTCCGCT GGTCCTGGCC CCGCGAGGAG CCCACCGCCT
AGCTCCAAAG AACCCCCCGA GGGCACGTGG ACCGAGGGAG CCCCTGTGAA GGCTGCGGAA
GACTCCGCGC GTCCCGAGCT CCCGGACTCT GCAGTGGGCC CGGGGTCCAG GGAGCCGCTA
AGGGTCCCTG AAGCTGTGGC CCTAGAGCGG CGGCGGGAGC AGGAAGAAAA GGAGGACATG
GAGACCCAGG CTGTGGCAAC GTCCCCCGAT GGCCGATACC TCAAGTTTGA CATCGAGATT
GGACGTGGCT CCTTCAAGAC GGTGTATCGA GGGCTAGACA CCGACACCAC AGTGGAGGTG
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTAGAGCTG AGCGGCAGCG CTTCTCAGAG
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA TGATTCGTGG
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
ACGCTCAAGA CGTACCTGAG GCGGTTCCGG GAGATGAAGC CGCGGGTCCT TCAGCGCTGG
AGCCGCCAAA TCCTGCGGGG ACTTCATTTC CTACACTCCC GGGTTCCTCC CATCCTGCAC
CGGGATCTCA AGTGCGACAA TGTCTTTATC ACGGGACCTA CTGGCTCTGT CAAAATCGGG
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTTGCCA AGAGTGTCAT CGGGACCCCG
GAATTCATGG CCCCCGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
TTCGGCATGT GCATGCTGGA GATGGCCACC TCTGAGTACC CGTACTCCGA GTGCCAGAAT
GCCGCGCAAA TCTACCGCAA GGTCACTTCG GGCAGAAAGC CGAACAGCTT CCACAAGGTG
AAGATACCCG AGGTGAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAACGAGAGG
TTCACCATCC AGGACCTCCT GGCCCACGCC TTCTTCCGCG AGGAGCGCGG TGTGCACGTG
GAACTAGCGG AGGAGGACGA CGGCGAGAAG CCGGGCCTCA AGCTCTGGCT GCGCATGGAG
GACGCGCGGC GCGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCCT GTTCCAGCTG
GGCCGGGACG CGGCCGAGGA GGTGGCACAG GAGATGGTGG CTCTGGGCTT GGTCTGTGAA
GCCGATTACC AGCCAGTGGC CCGTGCAGTA CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
CGTGAGAAGC TGCGTAAAGC AAGGGAATTG GAGGCACTCC CACCAGAGCC AGGACCTCCA
CCAGCAACTG TGCCCATGGC CCCCGGTCCC CCCAGTGTCT TCCCCCCTGA GCCTGAGGAG
CCAGAGGCAG ACCAGCACCA GCCCTTCCTT TTCCGCCACG CCAGCTACTC ATCTACCACT
TCGGATTGCG AGACTGATGG CTACCTCAGC TCCTCCGGCT TCCTGGATGC CTCAGACCCT
GCCCTTCAGC CCCCTGGGGG GGTGCCATCC AGCCTGGCTG AGTCCCATCT CTGCCTGCCC
TCGGCTTTTG CCCTATCCAT TCCACGTTCT GGCCCTGGAA GTGACTTTTC CCCCGGGGAC
AGCTATGCCT CAGATGCAGC TTCAGGCCTT AGCGATGTGG GAGAAGGGAT GGGACAAATG
AGGAGACCCC CAGGGAGGAA TCTCCGGCGC AGACCCCGAT CCCGGCTGCG GGTCACTAGT
GTCTCAGACC AGAATGACAG AGTGGTTGAG TGCCAGCTAC AGACCCATAA CAGCAAGATG
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCGGAAG AGATTGCAGC TGCCATGGAG
GAGCCAGCAC CATTACCTGC CCTGCCCGTC CCCCTCCCAG ACCCATCCAA TGAGCTCCAG
AGCAGCACCT CCCTGGAGCA CAGGAGCTGG ACAGCCTTCT CCACCTCCTC ATCTTCTCCT
GGAACTCCTT TGTCTCCTGG AAACCCATTT TCCCCTGGAA CCCCCATTTC CCCAGGTCCC
ATCTTCCCCA TCACTTCTCC CCCATGTCAT CCCAGCCCCT CCCCATTCTC CCCCATTTCT
TCCCAGGTCT CCTCAAATCC CTCTCCACAC CCCACCAGCT CTCCACTTCC ATTCTCCTCC
AGCACACCCG AGTTTCCGGT CCCACTCTCT CAGTGTCCCT GGAGTTCTCT CCCCACGACT
TCTCCACCTA CGTTCTCTCC CACTTGTTCT CAGGTCACTC TTAGTTCCCC TTTCTTTCCT
CCGTGCCCCT CCACTTCTTC CTTCCCCTCC ACCACAGCAG CCCCTCTCCT TTCTCTGGCT
AGTGCCTTCT CACTGGCTGT GATGACTGTG GCCCAGTCCC TGCTGTCCCC CTCACCTGGG
CTCCTTTCCC AGTCTCCTCC AGCCCCTCCT AGTCCCCTCC CTAGCCTGCC CCTTCCCCCT
CCCGTTGCTC CTGGTGGCCA GGAAAGCCCT TCACCCCACA CAGCTGAGGT GGAGAGTGAG
GCCTCACCAC CTCCTGCTCG GCCCCTCCCA GGGGAAGCCA GGCTGGCGCC CATCTCTGAA
GAGGGAAAGC CGCAGCTTGT TGGGCGTTTC CAAGTGACTT CATCCAAGGA ACCGGCTGAG
CCTCTTCCCT TGCAGCCAAC ATCCCCCACT CTCTCTGGTT CTCCAAAACC TTCAACCCCT
CAGCTCACTT CAGAGAGCTC AGATACAGAG GACAGTGCTG GAGGCGGGCC AGAGACCAGG
GAAGCTCTGG CTGAGAGCGA CCGTGCAGCT GAGGGTCTGG GGGCTGGAGT TGAGGAGGAA
GGAGATGATG GGAAGGAACC CCAAGTTGGG GGCAGCCCCC AACCCCTGAG CCATCCCAGC
CCAGTGTGGA TGAACTACTC CTACAGCAGC CTGTGTTTGA GCAGCGAGGA GTCAGAAAGC
AGTGGGGAAG ATGAGGAGTT CTGGGCTGAG CTGCAGAGTC TTCGGCAGAA GCACTTGTCA
GAGGTGGAAA CACTACAGAC ACTACAGAAA AAAGAAATTG AAGATTTGTA CAGCCGGCTG
GGGAAGCAGC CCCCACCGGG TATTGTGGCC CCAGCTGCTA TGCTGTCCAG CCGCCAGCGC
CGCCTCTCCA AGGGCAGCTT CCCCACCTCC CGCCGCAACA GCCTACAGCG CTCTGAGCCC
CCAGGCCCTG TGCTCTCCTT CCCCCATCCT GCTCCCAGGC ATCATGCGAA GGAACTCTCT
GAGTGGCAGC AGCACCGGCT CCCAGGAGCA GCGGGCAAGC AAGGGGGTGA CATTCGCCGG
GGATGTTGGC AGGATGTGAA TTCAGAACAG AAGCCATGTA TCTCCCCCAC ACCAGGGCCC
ACCATGGAGC TTGTGTTCTC AGAATCTGAT GCTTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011523436.1
CDS1027..4725
Misc Feature(1)1540..2322(+)
Misc Feature(2)1561..2322(+)
Misc Feature(3)1564..2049(+)
Misc Feature(4)1564..1989(+)
Misc Feature(5)1576..2049(+)
Misc Feature(6)1984..2049(+)
Misc Feature(7)2383..2493(+)
Translation

Target ORF information:

RefSeq Version XM_011525134
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011525134

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGTTGGCAT CCCCGGCCAC GGAGACCACC GTCCTCATGT CCCAGACTGA GGCCGACCTG 
GCCCTGCGGC CCCCGCCTCC TCTTGGCACC GCGGGGCAGC CCCGCCTCGG GCCCCCTCCT
CGCCGAGCGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC TCGTCTCAGC
CGCCGTAGCT CAGTCGACTT GGGGCTGCTG AGCTCTTGGT CCCTGCCAGC CTCACCCGCT
CCGGACCCCC CCGATCCTCC GGACTCCGCT GGTCCTGGCC CCGCGAGGAG CCCACCGCCT
AGCTCCAAAG AACCCCCCGA GGGCACGTGG ACCGAGGGAG CCCCTGTGAA GGCTGCGGAA
GACTCCGCGC GTCCCGAGCT CCCGGACTCT GCAGTGGGCC CGGGGTCCAG GGAGCCGCTA
AGGGTCCCTG AAGCTGTGGC CCTAGAGCGG CGGCGGGAGC AGGAAGAAAA GGAGGACATG
GAGACCCAGG CTGTGGCAAC GTCCCCCGAT GGCCGATACC TCAAGTTTGA CATCGAGATT
GGACGTGGCT CCTTCAAGAC GGTGTATCGA GGGCTAGACA CCGACACCAC AGTGGAGGTG
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTAGAGCTG AGCGGCAGCG CTTCTCAGAG
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA TGATTCGTGG
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
ACGCTCAAGA CGTACCTGAG GCGGTTCCGG GAGATGAAGC CGCGGGTCCT TCAGCGCTGG
AGCCGCCAAA TCCTGCGGGG ACTTCATTTC CTACACTCCC GGGTTCCTCC CATCCTGCAC
CGGGATCTCA AGTGCGACAA TGTCTTTATC ACGGGACCTA CTGGCTCTGT CAAAATCGGG
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTTGCCA AGAGTGTCAT CGGGACCCCG
GAATTCATGG CCCCCGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
TTCGGCATGT GCATGCTGGA GATGGCCACC TCTGAGTACC CGTACTCCGA GTGCCAGAAT
GCCGCGCAAA TCTACCGCAA GGTCACTTCG GGCAGAAAGC CGAACAGCTT CCACAAGGTG
AAGATACCCG AGGTGAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAACGAGAGG
TTCACCATCC AGGACCTCCT GGCCCACGCC TTCTTCCGCG AGGAGCGCGG TGTGCACGTG
GAACTAGCGG AGGAGGACGA CGGCGAGAAG CCGGGCCTCA AGCTCTGGCT GCGCATGGAG
GACGCGCGGC GCGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCCT GTTCCAGCTG
GGCCGGGACG CGGCCGAGGA GGTGGCACAG GAGATGGTGG CTCTGGGCTT GGTCTGTGAA
GCCGATTACC AGCCAGTGGC CCGTGCAGTA CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
CGTGAGAAGC TGCGTAAAGC AAGGGAATTG GAGGCACTCC CACCAGAGCC AGGACCTCCA
CCAGCAACTG TGCCCATGGC CCCCGGTCCC CCCAGTGTCT TCCCCCCTGA GCCTGAGGAG
CCAGAGGCAG ACCAGCACCA GCCCTTCCTT TTCCGCCACG CCAGCTACTC ATCTACCACT
TCGGATTGCG AGACTGATGG CTACCTCAGC TCCTCCGGCT TCCTGGATGC CTCAGACCCT
GCCCTTCAGC CCCCTGGGGG GGTGCCATCC AGCCTGGCTG AGTCCCATCT CTGCCTGCCC
TCGGCTTTTG CCCTATCCAT TCCACGTTCT GGCCCTGGAA GTGACTTTTC CCCCGGGGAC
AGCTATGCCT CAGATGCAGC TTCAGGCCTT AGCGATGTGG GAGAAGGGAT GGGACAAATG
AGGAGACCCC CAGGGAGGAA TCTCCGGCGC AGACCCCGAT CCCGGCTGCG GGTCACTAGT
GTCTCAGACC AGAATGACAG AGTGGTTGAG TGCCAGCTAC AGACCCATAA CAGCAAGATG
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCGGAAG AGATTGCAGC TGCCATGGAG
GAGCCAGCAC CATTACCTGC CCTGCCCGTC CCCCTCCCAG ACCCATCCAA TGAGCTCCAG
AGCAGCACCT CCCTGGAGCA CAGGAGCTGG ACAGCCTTCT CCACCTCCTC ATCTTCTCCT
GGAACTCCTT TGTCTCCTGG AAACCCATTT TCCCCTGGAA CCCCCATTTC CCCAGGTCCC
ATCTTCCCCA TCACTTCTCC CCCATGTCAT CCCAGCCCCT CCCCATTCTC CCCCATTTCT
TCCCAGGTCT CCTCAAATCC CTCTCCACAC CCCACCAGCT CTCCACTTCC ATTCTCCTCC
AGCACACCCG AGTTTCCGGT CCCACTCTCT CAGTGTCCCT GGAGTTCTCT CCCCACGACT
TCTCCACCTA CGTTCTCTCC CACTTGTTCT CAGGTCACTC TTAGTTCCCC TTTCTTTCCT
CCGTGCCCCT CCACTTCTTC CTTCCCCTCC ACCACAGCAG CCCCTCTCCT TTCTCTGGCT
AGTGCCTTCT CACTGGCTGT GATGACTGTG GCCCAGTCCC TGCTGTCCCC CTCACCTGGG
CTCCTTTCCC AGTCTCCTCC AGCCCCTCCT AGTCCCCTCC CTAGCCTGCC CCTTCCCCCT
CCCGTTGCTC CTGGTGGCCA GGAAAGCCCT TCACCCCACA CAGCTGAGGT GGAGAGTGAG
GCCTCACCAC CTCCTGCTCG GCCCCTCCCA GGGGAAGCCA GGCTGGCGCC CATCTCTGAA
GAGGGAAAGC CGCAGCTTGT TGGGCGTTTC CAAGTGACTT CATCCAAGGA ACCGGCTGAG
CCTCTTCCCT TGCAGCCAAC ATCCCCCACT CTCTCTGGTT CTCCAAAACC TTCAACCCCT
CAGCTCACTT CAGAGAGCTC AGATACAGAG GACAGTGCTG GAGGCGGGCC AGAGACCAGG
GAAGCTCTGG CTGAGAGCGA CCGTGCAGCT GAGGGTCTGG GGGCTGGAGT TGAGGAGGAA
GGAGATGATG GGAAGGAACC CCAAGTTGGG GGCAGCCCCC AACCCCTGAG CCATCCCAGC
CCAGTGTGGA TGAACTACTC CTACAGCAGC CTGTGTTTGA GCAGCGAGGA GTCAGAAAGC
AGTGGGGAAG ATGAGGAGTT CTGGGCTGAG CTGCAGAGTC TTCGGCAGAA GCACTTGTCA
GAGGTGGAAA CACTACAGAC ACTACAGAAA AAAGAAATTG AAGATTTGTA CAGCCGGCTG
GGGAAGCAGC CCCCACCGGG TATTGTGGCC CCAGCTGCTA TGCTGTCCAG CCGCCAGCGC
CGCCTCTCCA AGGGCAGCTT CCCCACCTCC CGCCGCAACA GCCTACAGCG CTCTGAGCCC
CCAGGCCCTG TGCTCTCCTT CCCCCATCCT GCTCCCAGGC ATCATGCGAA GGAACTCTCT
GAGTGGCAGC AGCACCGGCT CCCAGGAGCA GCGGGCAAGC AAGGGGGTGA CATTCGCCGG
GGATGTTGGC AGGATGTGAA TTCAGAACAG AAGCCATGTA TCTCCCCCAC ACCAGGGCCC
ACCATGGAGC TTGTGTTCTC AGAATCTGAT GCTTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71644
Clone ID Related Accession (Same CDS sequence) XM_011525135
Accession Version XM_011525135.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3690bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product serine/threonine-protein kinase WNK4 isoform X8
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGTTGGCAT CCCCGGCCAC GGAGACCACC GTCCTCATGT CCCAGACTGA GGCCGACCTG 
GCCCTGCGGC CCCCGCCTCC TCTTGGCACC GCGGGGCAGC CCCGCCTCGG GCCCCCTCCT
CGCCGAGCGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC TCGTCTCAGC
CGCCGTAGCT CAGTCGACTT GGGGCTGCTG AGCTCTTGGT CCCTGCCAGC CTCACCCGCT
CCGGACCCCC CCGATCCTCC GGACTCCGCT GGTCCTGGCC CCGCGAGGAG CCCACCGCCT
AGCTCCAAAG AACCCCCCGA GGGCACGTGG ACCGAGGGAG CCCCTGTGAA GGCTGCGGAA
GACTCCGCGC GTCCCGAGCT CCCGGACTCT GCAGTGGGCC CGGGGTCCAG GGAGCCGCTA
AGGGTCCCTG AAGCTGTGGC CCTAGAGCGG CGGCGGGAGC AGGAAGAAAA GGAGGACATG
GAGACCCAGG CTGTGGCAAC GTCCCCCGAT GGCCGATACC TCAAGTTTGA CATCGAGATT
GGACGTGGCT CCTTCAAGAC GGTGTATCGA GGGCTAGACA CCGACACCAC AGTGGAGGTG
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTAGAGCTG AGCGGCAGCG CTTCTCAGAG
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA TGATTCGTGG
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
ACGCTCAAGA CGTACCTGAG GCGGTTCCGG GAGATGAAGC CGCGGGTCCT TCAGCGCTGG
AGCCGCCAAA TCCTGCGGGG ACTTCATTTC CTACACTCCC GGGTTCCTCC CATCCTGCAC
CGGGATCTCA AGTGCGACAA TGTCTTTATC ACGGGACCTA CTGGCTCTGT CAAAATCGGG
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTTGCCA AGAGTGTCAT CGGGACCCCG
GAATTCATGG CCCCCGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
TTCGGCATGT GCATGCTGGA GATGGCCACC TCTGAGTACC CGTACTCCGA GTGCCAGAAT
GCCGCGCAAA TCTACCGCAA GGTCACTTCG GGCAGAAAGC CGAACAGCTT CCACAAGGTG
AAGATACCCG AGGTGAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAACGAGAGG
TTCACCATCC AGGACCTCCT GGCCCACGCC TTCTTCCGCG AGGAGCGCGG TGTGCACGTG
GAACTAGCGG AGGAGGACGA CGGCGAGAAG CCGGGCCTCA AGCTCTGGCT GCGCATGGAG
GACGCGCGGC GCGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCCT GTTCCAGCTG
GGCCGGGACG CGGCCGAGGA GGTGGCACAG GAGATGGTGG CTCTGGGCTT GGTCTGTGAA
GCCGATTACC AGCCAGTGGC CCGTGCAGTA CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
CGTGAGAAGC TGCGTAAAGC AAGGGAATTG GAGGCACTCC CACCAGAGCC AGGACCTCCA
CCAGCAACTG TGCCCATGGC CCCCGGTCCC CCCAGTGTCT TCCCCCCTGA GCCTGAGGAG
CCAGAGGCAG ACCAGCACCA GCCCTTCCTT TTCCGCCACG CCAGCTACTC ATCTACCACT
TCGGATTGCG AGACTGATGG CTACCTCAGC TCCTCCGGCT TCCTGGATGC CTCAGACCCT
GCCCTTCAGC CCCCTGGGGG GGTGCCATCC AGCCTGGCTG AGTCCCATCT CTGCCTGCCC
TCGGCTTTTG CCCTATCCAT TCCACGTTCT GGCCCTGGAA GTGACTTTTC CCCCGGGGAC
AGCTATGCCT CAGATGCAGC TTCAGGCCTT AGCGATGTGG GAGAAGGGAT GGGACAAATG
AGGAGACCCC CAGGGAGGAA TCTCCGGCGC AGACCCCGAT CCCGGCTGCG GGTCACTAGT
GTCTCAGACC AGAATGACAG AGTGGTTGAG TGCCAGCTAC AGACCCATAA CAGCAAGATG
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCGGAAG AGATTGCAGC TGCCATGGTA
TATAACGAGT TCATTCTGCC TTCGGAGCGA GATGGATTTC TCAGACGGAT TCGGGAGATT
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GACACTGGCC CCATGGAGGC TGCTGAAGAC
ACCCTAAGCC CCCAGGAGGA GCCAGCACCA TTACCTGCCC TGCCCGTCCC CCTCCCAGAC
CCATCCAATG AAGAGCTCCA GAGCAGCACC TCCCTGGAGC ACAGGAGCTG GACAGCCTTC
TCCACCTCCT CATCTTCTCC TGGAACTCCT TTGTCTCCTG GAAACCCATT TTCCCCTGGA
ACCCCCATTT CCCCAGGTCC CATCTTCCCC ATCACTTCTC CCCCATGTCA TCCCAGCCCC
TCCCCATTCT CCCCCATTTC TTCCCAGGTC TCCTCAAATC CCTCTCCACA CCCCACCAGC
TCTCCACTTC CATTCTCCTC CAGCACACCC GAGTTTCCGG TCCCACTCTC TCAGTGTCCC
TGGAGTTCTC TCCCCACGAC TTCTCCACCT ACGTTCTCTC CCACTTGTTC TCAGGTCACT
CTTAGTTCCC CTTTCTTTCC TCCGTGCCCC TCCACTTCTT CCTTCCCCTC CACCACAGCA
GCCCCTCTCC TTTCTCTGGC TAGTGCCTTC TCACTGGCTG TGATGACTGT GGCCCAGTCC
CTGCTGTCCC CCTCACCTGG GCTCCTTTCC CAGTCTCCTC CAGCCCCTCC TAGTCCCCTC
CCTAGCCTGC CCCTTCCCCC TCCCGTTGCT CCTGGTGGCC AGGAAAGCCC TTCACCCCAC
ACAGCTGAGG TGGAGAGTGA GGCCTCACCA CCTCCTGCTC GGCCCCTCCC AGGGGAAGCC
AGGCTGGCGC CCATCTCTGA AGAGGGAAAG CCGCAGCTTG TTGGGCGTTT CCAAGTGACT
TCATCCAAGG AACCGGCTGA GCCTCTTCCC TTGCAGCCAA CATCCCCCAC TCTCTCTGGT
TCTCCAAAAC CTTCAACCCC TCAGCTCACT TCAGAGAGCT CAGATACAGA GGACAGTGCT
GGAGGCGGGC CAGAGACCAG GGAAGCTCTG GCTGAGAGCG ACCGTGCAGC TGAGGGTCTG
GGGGCTGGAG TTGAGGAGGA AGGAGATGAT GGGAAGGAAC CCCAAGTTGG GGGCAGCCCC
CAACCCCTGA GCCATCCCAG CCCAGTGTGG ATGAACTACT CCTACAGCAG CCTGTGTTTG
AGCAGCGAGG AGTCAGAAAG CAGTGGGGAA GATGAGGAGT TCTGGGCTGA GCTGCAGAGT
CTTCGGCAGA AGCACTTGTC AGAGGTGGAA ACACTACAGA CACTACAGAA AAAAGAAATT
GAAGATTTGT ACAGCCGGCT GGGGAAGCAG CCCCCACCGG GTATTGTGGC CCCAGCTGCT
ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC AAGGGCAGCT TCCCCACCTC CCGCCGCAAC
AGCCTACAGC GCTCTGAGCC CCCAGGCCCT GGCATCATGC GAAGGAACTC TCTGAGTGGC
AGCAGCACCG GCTCCCAGGA GCAGCGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011523437.1
CDS1027..4716
Misc Feature(1)1540..2322(+)
Misc Feature(2)1561..2322(+)
Misc Feature(3)1564..2049(+)
Misc Feature(4)1564..1989(+)
Misc Feature(5)1576..2049(+)
Misc Feature(6)1984..2049(+)
Misc Feature(7)2383..2493(+)
Translation

Target ORF information:

RefSeq Version XM_011525135
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011525135

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGTTGGCAT CCCCGGCCAC GGAGACCACC GTCCTCATGT CCCAGACTGA GGCCGACCTG 
GCCCTGCGGC CCCCGCCTCC TCTTGGCACC GCGGGGCAGC CCCGCCTCGG GCCCCCTCCT
CGCCGAGCGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC TCGTCTCAGC
CGCCGTAGCT CAGTCGACTT GGGGCTGCTG AGCTCTTGGT CCCTGCCAGC CTCACCCGCT
CCGGACCCCC CCGATCCTCC GGACTCCGCT GGTCCTGGCC CCGCGAGGAG CCCACCGCCT
AGCTCCAAAG AACCCCCCGA GGGCACGTGG ACCGAGGGAG CCCCTGTGAA GGCTGCGGAA
GACTCCGCGC GTCCCGAGCT CCCGGACTCT GCAGTGGGCC CGGGGTCCAG GGAGCCGCTA
AGGGTCCCTG AAGCTGTGGC CCTAGAGCGG CGGCGGGAGC AGGAAGAAAA GGAGGACATG
GAGACCCAGG CTGTGGCAAC GTCCCCCGAT GGCCGATACC TCAAGTTTGA CATCGAGATT
GGACGTGGCT CCTTCAAGAC GGTGTATCGA GGGCTAGACA CCGACACCAC AGTGGAGGTG
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTAGAGCTG AGCGGCAGCG CTTCTCAGAG
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA TGATTCGTGG
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
ACGCTCAAGA CGTACCTGAG GCGGTTCCGG GAGATGAAGC CGCGGGTCCT TCAGCGCTGG
AGCCGCCAAA TCCTGCGGGG ACTTCATTTC CTACACTCCC GGGTTCCTCC CATCCTGCAC
CGGGATCTCA AGTGCGACAA TGTCTTTATC ACGGGACCTA CTGGCTCTGT CAAAATCGGG
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTTGCCA AGAGTGTCAT CGGGACCCCG
GAATTCATGG CCCCCGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
TTCGGCATGT GCATGCTGGA GATGGCCACC TCTGAGTACC CGTACTCCGA GTGCCAGAAT
GCCGCGCAAA TCTACCGCAA GGTCACTTCG GGCAGAAAGC CGAACAGCTT CCACAAGGTG
AAGATACCCG AGGTGAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAACGAGAGG
TTCACCATCC AGGACCTCCT GGCCCACGCC TTCTTCCGCG AGGAGCGCGG TGTGCACGTG
GAACTAGCGG AGGAGGACGA CGGCGAGAAG CCGGGCCTCA AGCTCTGGCT GCGCATGGAG
GACGCGCGGC GCGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCCT GTTCCAGCTG
GGCCGGGACG CGGCCGAGGA GGTGGCACAG GAGATGGTGG CTCTGGGCTT GGTCTGTGAA
GCCGATTACC AGCCAGTGGC CCGTGCAGTA CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
CGTGAGAAGC TGCGTAAAGC AAGGGAATTG GAGGCACTCC CACCAGAGCC AGGACCTCCA
CCAGCAACTG TGCCCATGGC CCCCGGTCCC CCCAGTGTCT TCCCCCCTGA GCCTGAGGAG
CCAGAGGCAG ACCAGCACCA GCCCTTCCTT TTCCGCCACG CCAGCTACTC ATCTACCACT
TCGGATTGCG AGACTGATGG CTACCTCAGC TCCTCCGGCT TCCTGGATGC CTCAGACCCT
GCCCTTCAGC CCCCTGGGGG GGTGCCATCC AGCCTGGCTG AGTCCCATCT CTGCCTGCCC
TCGGCTTTTG CCCTATCCAT TCCACGTTCT GGCCCTGGAA GTGACTTTTC CCCCGGGGAC
AGCTATGCCT CAGATGCAGC TTCAGGCCTT AGCGATGTGG GAGAAGGGAT GGGACAAATG
AGGAGACCCC CAGGGAGGAA TCTCCGGCGC AGACCCCGAT CCCGGCTGCG GGTCACTAGT
GTCTCAGACC AGAATGACAG AGTGGTTGAG TGCCAGCTAC AGACCCATAA CAGCAAGATG
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCGGAAG AGATTGCAGC TGCCATGGTA
TATAACGAGT TCATTCTGCC TTCGGAGCGA GATGGATTTC TCAGACGGAT TCGGGAGATT
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GACACTGGCC CCATGGAGGC TGCTGAAGAC
ACCCTAAGCC CCCAGGAGGA GCCAGCACCA TTACCTGCCC TGCCCGTCCC CCTCCCAGAC
CCATCCAATG AAGAGCTCCA GAGCAGCACC TCCCTGGAGC ACAGGAGCTG GACAGCCTTC
TCCACCTCCT CATCTTCTCC TGGAACTCCT TTGTCTCCTG GAAACCCATT TTCCCCTGGA
ACCCCCATTT CCCCAGGTCC CATCTTCCCC ATCACTTCTC CCCCATGTCA TCCCAGCCCC
TCCCCATTCT CCCCCATTTC TTCCCAGGTC TCCTCAAATC CCTCTCCACA CCCCACCAGC
TCTCCACTTC CATTCTCCTC CAGCACACCC GAGTTTCCGG TCCCACTCTC TCAGTGTCCC
TGGAGTTCTC TCCCCACGAC TTCTCCACCT ACGTTCTCTC CCACTTGTTC TCAGGTCACT
CTTAGTTCCC CTTTCTTTCC TCCGTGCCCC TCCACTTCTT CCTTCCCCTC CACCACAGCA
GCCCCTCTCC TTTCTCTGGC TAGTGCCTTC TCACTGGCTG TGATGACTGT GGCCCAGTCC
CTGCTGTCCC CCTCACCTGG GCTCCTTTCC CAGTCTCCTC CAGCCCCTCC TAGTCCCCTC
CCTAGCCTGC CCCTTCCCCC TCCCGTTGCT CCTGGTGGCC AGGAAAGCCC TTCACCCCAC
ACAGCTGAGG TGGAGAGTGA GGCCTCACCA CCTCCTGCTC GGCCCCTCCC AGGGGAAGCC
AGGCTGGCGC CCATCTCTGA AGAGGGAAAG CCGCAGCTTG TTGGGCGTTT CCAAGTGACT
TCATCCAAGG AACCGGCTGA GCCTCTTCCC TTGCAGCCAA CATCCCCCAC TCTCTCTGGT
TCTCCAAAAC CTTCAACCCC TCAGCTCACT TCAGAGAGCT CAGATACAGA GGACAGTGCT
GGAGGCGGGC CAGAGACCAG GGAAGCTCTG GCTGAGAGCG ACCGTGCAGC TGAGGGTCTG
GGGGCTGGAG TTGAGGAGGA AGGAGATGAT GGGAAGGAAC CCCAAGTTGG GGGCAGCCCC
CAACCCCTGA GCCATCCCAG CCCAGTGTGG ATGAACTACT CCTACAGCAG CCTGTGTTTG
AGCAGCGAGG AGTCAGAAAG CAGTGGGGAA GATGAGGAGT TCTGGGCTGA GCTGCAGAGT
CTTCGGCAGA AGCACTTGTC AGAGGTGGAA ACACTACAGA CACTACAGAA AAAAGAAATT
GAAGATTTGT ACAGCCGGCT GGGGAAGCAG CCCCCACCGG GTATTGTGGC CCCAGCTGCT
ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC AAGGGCAGCT TCCCCACCTC CCGCCGCAAC
AGCCTACAGC GCTCTGAGCC CCCAGGCCCT GGCATCATGC GAAGGAACTC TCTGAGTGGC
AGCAGCACCG GCTCCCAGGA GCAGCGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50598
Clone ID Related Accession (Same CDS sequence) XM_006722021
Accession Version XM_006722021.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2832bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product serine/threonine-protein kinase WNK4 isoform X9
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
ATGTCTTTAT CACGGGACCT ACTGGCTCTG TCAAAATCGG GGACCTGGGC CTGGCCACGC 
TCAAGCGCGC CTCCTTTGCC AAGAGTGTCA TCGGGACCCC GGAATTCATG GCCCCCGAGA
TGTACGAGGA AAAGTACGAT GAGGCCGTGG ACGTGTACGC GTTCGGCATG TGCATGCTGG
AGATGGCCAC CTCTGAGTAC CCGTACTCCG AGTGCCAGAA TGCCGCGCAA ATCTACCGCA
AGGTCACTTC GGTTCACCAT CCAGGACCTC CTGGCCCACG CCTTCTTCCG CGAGGAGCGC
GGTGTGCACG TGGAACTAGC GGAGGAGGAC GACGGCGAGA AGCCGGGCCT CAAGCTCTGG
CTGCGCATGG AGGACGCGCG GCGCGGGGGG CGCCCACGGG ACAACCAGGC CATCGAGTTC
CTGTTCCAGC TGGGCCGGGA CGCGGCCGAG GAGGTGGCAC AGGAGATGGT GGCTCTGGGC
TTGGTCTGTG AAGCCGATTA CCAGCCAGTG GCCCGTGCAG TACGTGAACG GGTTGCTGCC
ATCCAGCGAA AGCGTGAGAA GCTGCGTAAA GCAAGGGAAT TGGAGGCACT CCCACCAGAG
CCAGGACCTC CACCAGCAAC TGTGCCCATG GCCCCCGGTC CCCCCAGTGT CTTCCCCCCT
GAGCCTGAGG AGCCAGAGGC AGACCAGCAC CAGCCCTTCC TTTTCCGCCA CGCCAGCTAC
TCATCTACCA CTTCGGATTG CGAGACTGAT GGCTACCTCA GCTCCTCCGG CTTCCTGGAT
GCCTCAGACC CTGCCCTTCA GCCCCCTGGG GGGGTGCCAT CCAGCCTGGC TGAGTCCCAT
CTCTGCCTGC CCTCGGCTTT TGCCCTATCC ATTCCACGTT CTGGCCCTGG AAGTGACTTT
TCCCCCGGGG ACAGCTATGC CTCAGATGCA GCTTCAGGCC TTAGCGATGT GGGAGAAGGG
ATGGGACAAA TGAGGAGACC CCCAGGGAGG AATCTCCGGC GCAGACCCCG ATCCCGGCTG
CGGGTCACTA GTGTCTCAGA CCAGAATGAC AGAGTGGTTG AGTGCCAGCT ACAGACCCAT
AACAGCAAGA TGGTGACCTT CCGATTTGAT CTGGATGGGG ACAGCCCGGA AGAGATTGCA
GCTGCCATGG TATATAACGA GTTCATTCTG CCTTCGGAGC GAGATGGATT TCTCAGACGG
ATTCGGGAGA TTATCCAGCG AGTGGAGACC CTGTTGAAGA GAGACACTGG CCCCATGGAG
GCTGCTGAAG ACACCCTAAG CCCCCAGGAG GAGCCAGCAC CATTACCTGC CCTGCCCGTC
CCCCTCCCAG ACCCATCCAA TGAAGAGCTC CAGAGCAGCA CCTCCCTGGA GCACAGGAGC
TGGACAGCCT TCTCCACCTC CTCATCTTCT CCTGGAACTC CTTTGTCTCC TGGAAACCCA
TTTTCCCCTG GAACCCCCAT TTCCCCAGGT CCCATCTTCC CCATCACTTC TCCCCCATGT
CATCCCAGCC CCTCCCCATT CTCCCCCATT TCTTCCCAGG TCTCCTCAAA TCCCTCTCCA
CACCCCACCA GCTCTCCACT TCCATTCTCC TCCAGCACAC CCGAGTTTCC GGTCCCACTC
TCTCAGTGTC CCTGGAGTTC TCTCCCCACG ACTTCTCCAC CTACGTTCTC TCCCACTTGT
TCTCAGGTCA CTCTTAGTTC CCCTTTCTTT CCTCCGTGCC CCTCCACTTC TTCCTTCCCC
TCCACCACAG CAGCCCCTCT CCTTTCTCTG GCTAGTGCCT TCTCACTGGC TGTGATGACT
GTGGCCCAGT CCCTGCTGTC CCCCTCACCT GGGCTCCTTT CCCAGTCTCC TCCAGCCCCT
CCTAGTCCCC TCCCTAGCCT GCCCCTTCCC CCTCCCGTTG CTCCTGGTGG CCAGGAAAGC
CCTTCACCCC ACACAGCTGA GGTGGAGAGT GAGGCCTCAC CACCTCCTGC TCGGCCCCTC
CCAGGGGAAG CCAGGCTGGC GCCCATCTCT GAAGAGGGAA AGCCGCAGCT TGTTGGGCGT
TTCCAAGTGA CTTCATCCAA GGAACCGGCT GAGCCTCTTC CCTTGCAGCC AACATCCCCC
ACTCTCTCTG GTTCTCCAAA ACCTTCAACC CCTCAGCTCA CTTCAGAGAG CTCAGATACA
GAGGACAGTG CTGGAGGCGG GCCAGAGACC AGGGAAGCTC TGGCTGAGAG CGACCGTGCA
GCTGAGGGTC TGGGGGCTGG AGTTGAGGAG GAAGGAGATG ATGGGAAGGA ACCCCAAGTT
GGGGGCAGCC CCCAACCCCT GAGCCATCCC AGCCCAGTGT GGATGAACTA CTCCTACAGC
AGCCTGTGTT TGAGCAGCGA GGAGTCAGAA AGCAGTGGGG AAGATGAGGA GTTCTGGGCT
GAGCTGCAGA GTCTTCGGCA GAAGCACTTG TCAGAGGTGG AAACACTACA GACACTACAG
AAAAAAGAAA TTGAAGATTT GTACAGCCGG CTGGGGAAGC AGCCCCCACC GGGTATTGTG
GCCCCAGCTG CTATGCTGTC CAGCCGCCAG CGCCGCCTCT CCAAGGGCAG CTTCCCCACC
TCCCGCCGCA ACAGCCTACA GCGCTCTGAG CCCCCAGGCC CTGTGCTCTC CTTCCCCCAT
CCTGCTCCCA GGCATCATGC GAAGGAACTC TCTGAGTGGC AGCAGCACCG GCTCCCAGGA
GCAGCGGGCA AGCAAGGGGG TGACATTCGC CGGGGATGTT GGCAGGATGT GAATTCAGAA
CAGAAGCCAT GTATCTCCCC CACACCAGGG CCCACCATGG AGCTTGTGTT CTCAGAATCT
GATGCTTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006722084.1
CDS129..2960
Misc Feature(1)477..587(+)
Misc Feature(2)2067..>2444(+)
Translation

Target ORF information:

RefSeq Version XM_006722021
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006722021

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
ATGTCTTTAT CACGGGACCT ACTGGCTCTG TCAAAATCGG GGACCTGGGC CTGGCCACGC 
TCAAGCGCGC CTCCTTTGCC AAGAGTGTCA TCGGGACCCC GGAATTCATG GCCCCCGAGA
TGTACGAGGA AAAGTACGAT GAGGCCGTGG ACGTGTACGC GTTCGGCATG TGCATGCTGG
AGATGGCCAC CTCTGAGTAC CCGTACTCCG AGTGCCAGAA TGCCGCGCAA ATCTACCGCA
AGGTCACTTC GGTTCACCAT CCAGGACCTC CTGGCCCACG CCTTCTTCCG CGAGGAGCGC
GGTGTGCACG TGGAACTAGC GGAGGAGGAC GACGGCGAGA AGCCGGGCCT CAAGCTCTGG
CTGCGCATGG AGGACGCGCG GCGCGGGGGG CGCCCACGGG ACAACCAGGC CATCGAGTTC
CTGTTCCAGC TGGGCCGGGA CGCGGCCGAG GAGGTGGCAC AGGAGATGGT GGCTCTGGGC
TTGGTCTGTG AAGCCGATTA CCAGCCAGTG GCCCGTGCAG TACGTGAACG GGTTGCTGCC
ATCCAGCGAA AGCGTGAGAA GCTGCGTAAA GCAAGGGAAT TGGAGGCACT CCCACCAGAG
CCAGGACCTC CACCAGCAAC TGTGCCCATG GCCCCCGGTC CCCCCAGTGT CTTCCCCCCT
GAGCCTGAGG AGCCAGAGGC AGACCAGCAC CAGCCCTTCC TTTTCCGCCA CGCCAGCTAC
TCATCTACCA CTTCGGATTG CGAGACTGAT GGCTACCTCA GCTCCTCCGG CTTCCTGGAT
GCCTCAGACC CTGCCCTTCA GCCCCCTGGG GGGGTGCCAT CCAGCCTGGC TGAGTCCCAT
CTCTGCCTGC CCTCGGCTTT TGCCCTATCC ATTCCACGTT CTGGCCCTGG AAGTGACTTT
TCCCCCGGGG ACAGCTATGC CTCAGATGCA GCTTCAGGCC TTAGCGATGT GGGAGAAGGG
ATGGGACAAA TGAGGAGACC CCCAGGGAGG AATCTCCGGC GCAGACCCCG ATCCCGGCTG
CGGGTCACTA GTGTCTCAGA CCAGAATGAC AGAGTGGTTG AGTGCCAGCT ACAGACCCAT
AACAGCAAGA TGGTGACCTT CCGATTTGAT CTGGATGGGG ACAGCCCGGA AGAGATTGCA
GCTGCCATGG TATATAACGA GTTCATTCTG CCTTCGGAGC GAGATGGATT TCTCAGACGG
ATTCGGGAGA TTATCCAGCG AGTGGAGACC CTGTTGAAGA GAGACACTGG CCCCATGGAG
GCTGCTGAAG ACACCCTAAG CCCCCAGGAG GAGCCAGCAC CATTACCTGC CCTGCCCGTC
CCCCTCCCAG ACCCATCCAA TGAAGAGCTC CAGAGCAGCA CCTCCCTGGA GCACAGGAGC
TGGACAGCCT TCTCCACCTC CTCATCTTCT CCTGGAACTC CTTTGTCTCC TGGAAACCCA
TTTTCCCCTG GAACCCCCAT TTCCCCAGGT CCCATCTTCC CCATCACTTC TCCCCCATGT
CATCCCAGCC CCTCCCCATT CTCCCCCATT TCTTCCCAGG TCTCCTCAAA TCCCTCTCCA
CACCCCACCA GCTCTCCACT TCCATTCTCC TCCAGCACAC CCGAGTTTCC GGTCCCACTC
TCTCAGTGTC CCTGGAGTTC TCTCCCCACG ACTTCTCCAC CTACGTTCTC TCCCACTTGT
TCTCAGGTCA CTCTTAGTTC CCCTTTCTTT CCTCCGTGCC CCTCCACTTC TTCCTTCCCC
TCCACCACAG CAGCCCCTCT CCTTTCTCTG GCTAGTGCCT TCTCACTGGC TGTGATGACT
GTGGCCCAGT CCCTGCTGTC CCCCTCACCT GGGCTCCTTT CCCAGTCTCC TCCAGCCCCT
CCTAGTCCCC TCCCTAGCCT GCCCCTTCCC CCTCCCGTTG CTCCTGGTGG CCAGGAAAGC
CCTTCACCCC ACACAGCTGA GGTGGAGAGT GAGGCCTCAC CACCTCCTGC TCGGCCCCTC
CCAGGGGAAG CCAGGCTGGC GCCCATCTCT GAAGAGGGAA AGCCGCAGCT TGTTGGGCGT
TTCCAAGTGA CTTCATCCAA GGAACCGGCT GAGCCTCTTC CCTTGCAGCC AACATCCCCC
ACTCTCTCTG GTTCTCCAAA ACCTTCAACC CCTCAGCTCA CTTCAGAGAG CTCAGATACA
GAGGACAGTG CTGGAGGCGG GCCAGAGACC AGGGAAGCTC TGGCTGAGAG CGACCGTGCA
GCTGAGGGTC TGGGGGCTGG AGTTGAGGAG GAAGGAGATG ATGGGAAGGA ACCCCAAGTT
GGGGGCAGCC CCCAACCCCT GAGCCATCCC AGCCCAGTGT GGATGAACTA CTCCTACAGC
AGCCTGTGTT TGAGCAGCGA GGAGTCAGAA AGCAGTGGGG AAGATGAGGA GTTCTGGGCT
GAGCTGCAGA GTCTTCGGCA GAAGCACTTG TCAGAGGTGG AAACACTACA GACACTACAG
AAAAAAGAAA TTGAAGATTT GTACAGCCGG CTGGGGAAGC AGCCCCCACC GGGTATTGTG
GCCCCAGCTG CTATGCTGTC CAGCCGCCAG CGCCGCCTCT CCAAGGGCAG CTTCCCCACC
TCCCGCCGCA ACAGCCTACA GCGCTCTGAG CCCCCAGGCC CTGTGCTCTC CTTCCCCCAT
CCTGCTCCCA GGCATCATGC GAAGGAACTC TCTGAGTGGC AGCAGCACCG GCTCCCAGGA
GCAGCGGGCA AGCAAGGGGG TGACATTCGC CGGGGATGTT GGCAGGATGT GAATTCAGAA
CAGAAGCCAT GTATCTCCCC CACACCAGGG CCCACCATGG AGCTTGTGTT CTCAGAATCT
GATGCTTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50599
Clone ID Related Accession (Same CDS sequence) XM_006722022
Accession Version XM_006722022.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2724bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product serine/threonine-protein kinase WNK4 isoform X10
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGTCTTTAT CACGGGACCT ACTGGCTCTG TCAAAATCGG GGACCTGGGC CTGGCCACGC 
TCAAGCGCGC CTCCTTTGCC AAGAGTGTCA TCGGGACCCC GGAATTCATG GCCCCCGAGA
TGTACGAGGA AAAGTACGAT GAGGCCGTGG ACGTGTACGC GTTCGGCATG TGCATGCTGG
AGATGGCCAC CTCTGAGTAC CCGTACTCCG AGTGCCAGAA TGCCGCGCAA ATCTACCGCA
AGGTCACTTC GGTTCACCAT CCAGGACCTC CTGGCCCACG CCTTCTTCCG CGAGGAGCGC
GGTGTGCACG TGGAACTAGC GGAGGAGGAC GACGGCGAGA AGCCGGGCCT CAAGCTCTGG
CTGCGCATGG AGGACGCGCG GCGCGGGGGG CGCCCACGGG ACAACCAGGC CATCGAGTTC
CTGTTCCAGC TGGGCCGGGA CGCGGCCGAG GAGGTGGCAC AGGAGATGGT GGCTCTGGGC
TTGGTCTGTG AAGCCGATTA CCAGCCAGTG GCCCGTGCAG TACGTGAACG GGTTGCTGCC
ATCCAGCGAA AGCGTGAGAA GCTGCGTAAA GCAAGGGAAT TGGAGGCACT CCCACCAGAG
CCAGGACCTC CACCAGCAAC TGTGCCCATG GCCCCCGGTC CCCCCAGTGT CTTCCCCCCT
GAGCCTGAGG AGCCAGAGGC AGACCAGCAC CAGCCCTTCC TTTTCCGCCA CGCCAGCTAC
TCATCTACCA CTTCGGATTG CGAGACTGAT GGCTACCTCA GCTCCTCCGG CTTCCTGGAT
GCCTCAGACC CTGCCCTTCA GCCCCCTGGG GGGGTGCCAT CCAGCCTGGC TGAGTCCCAT
CTCTGCCTGC CCTCGGCTTT TGCCCTATCC ATTCCACGTT CTGGCCCTGG AAGTGACTTT
TCCCCCGGGG ACAGCTATGC CTCAGATGCA GCTTCAGGCC TTAGCGATGT GGGAGAAGGG
ATGGGACAAA TGAGGAGACC CCCAGGGAGG AATCTCCGGC GCAGACCCCG ATCCCGGCTG
CGGGTCACTA GTGTCTCAGA CCAGAATGAC AGAGTGGTTG AGTGCCAGCT ACAGACCCAT
AACAGCAAGA TGGTGACCTT CCGATTTGAT CTGGATGGGG ACAGCCCGGA AGAGATTGCA
GCTGCCATGG TATATAACGA GTTCATTCTG CCTTCGGAGC GAGATGGATT TCTCAGACGG
ATTCGGGAGA TTATCCAGCG AGTGGAGACC CTGTTGAAGA GAGACACTGG CCCCATGGAG
GCTGCTGAAG ACACCCTAAG CCCCCAGGAG GAGCCAGCAC CATTACCTGC CCTGCCCGTC
CCCCTCCCAG ACCCATCCAA TGAAGAGCTC CAGAGCAGCA CCTCCCTGGA GCACAGGAGC
TGGACAGCCT TCTCCACCTC CTCATCTTCT CCTGGAACTC CTTTGTCTCC TGGAAACCCA
TTTTCCCCTG GAACCCCCAT TTCCCCAGGT CCCATCTTCC CCATCACTTC TCCCCCATGT
CATCCCAGCC CCTCCCCATT CTCCCCCATT TCTTCCCAGG TCTCCTCAAA TCCCTCTCCA
CACCCCACCA GCTCTCCACT TCCATTCTCC TCCAGCACAC CCGAGTTTCC GGTCCCACTC
TCTCAGTGTC CCTGGAGTTC TCTCCCCACG ACTTCTCCAC CTACGTTCTC TCCCACTTGT
TCTCAGGTCA CTCTTAGTTC CCCTTTCTTT CCTCCGTGCC CCTCCACTTC TTCCTTCCCC
TCCACCACAG CAGCCCCTCT CCTTTCTCTG GCTAGTGCCT TCTCACTGGC TGTGATGACT
GTGGCCCAGT CCCTGCTGTC CCCCTCACCT GGGCTCCTTT CCCAGTCTCC TCCAGCCCCT
CCTAGTCCCC TCCCTAGCCT GCCCCTTCCC CCTCCCGTTG CTCCTGGTGG CCAGGAAAGC
CCTTCACCCC ACACAGCTGA GGTGGAGAGT GAGGCCTCAC CACCTCCTGC TCGGCCCCTC
CCAGGGGAAG CCAGGCTGGC GCCCATCTCT GAAGAGGGAA AGCCGCAGCT TGTTGGGCGT
TTCCAAGTGA CTTCATCCAA GGAACCGGCT GAGCCTCTTC CCTTGCAGCC AACATCCCCC
ACTCTCTCTG GTTCTCCAAA ACCTTCAACC CCTCAGCTCA CTTCAGAGAG CTCAGATACA
GAGGACAGTG CTGGAGGCGG GCCAGAGACC AGGGAAGCTC TGGCTGAGAG CGACCGTGCA
GCTGAGGGTC TGGGGGCTGG AGTTGAGGAG GAAGGAGATG ATGGGAAGGA ACCCCAAGTT
GGGGGCAGCC CCCAACCCCT GAGCCATCCC AGCCCAGTGT GGATGAACTA CTCCTACAGC
AGCCTGTGTT TGAGCAGCGA GGAGTCAGAA AGCAGTGGGG AAGATGAGGA GTTCTGGGCT
GAGCTGCAGA GTCTTCGGCA GAAGCACTTG TCAGAGGTGG AAACACTACA GACACTACAG
AAAAAAGAAA TTGAAGATTT GTACAGCCGG CTGGGGAAGC AGCCCCCACC GGGTATTGTG
GCCCCAGCTG CTATGCTGTC CAGCCGCCAG CGCCGCCTCT CCAAGGGCAG CTTCCCCACC
TCCCGCCGCA ACAGCCTACA GCGCTCTGAG CCCCCAGGCC CTGGCATCAT GCGAAGGAAC
TCTCTGAGTG GCAGCAGCAC CGGCTCCCAG GAGCAGCGGG CAAGCAAGGG GGTGACATTC
GCCGGGGATG TTGGCAGGAT GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006722085.1
CDS129..2852
Misc Feature(1)477..587(+)
Misc Feature(2)2067..>2444(+)
Translation

Target ORF information:

RefSeq Version XM_006722022
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant X10, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006722022

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGTCTTTAT CACGGGACCT ACTGGCTCTG TCAAAATCGG GGACCTGGGC CTGGCCACGC 
TCAAGCGCGC CTCCTTTGCC AAGAGTGTCA TCGGGACCCC GGAATTCATG GCCCCCGAGA
TGTACGAGGA AAAGTACGAT GAGGCCGTGG ACGTGTACGC GTTCGGCATG TGCATGCTGG
AGATGGCCAC CTCTGAGTAC CCGTACTCCG AGTGCCAGAA TGCCGCGCAA ATCTACCGCA
AGGTCACTTC GGTTCACCAT CCAGGACCTC CTGGCCCACG CCTTCTTCCG CGAGGAGCGC
GGTGTGCACG TGGAACTAGC GGAGGAGGAC GACGGCGAGA AGCCGGGCCT CAAGCTCTGG
CTGCGCATGG AGGACGCGCG GCGCGGGGGG CGCCCACGGG ACAACCAGGC CATCGAGTTC
CTGTTCCAGC TGGGCCGGGA CGCGGCCGAG GAGGTGGCAC AGGAGATGGT GGCTCTGGGC
TTGGTCTGTG AAGCCGATTA CCAGCCAGTG GCCCGTGCAG TACGTGAACG GGTTGCTGCC
ATCCAGCGAA AGCGTGAGAA GCTGCGTAAA GCAAGGGAAT TGGAGGCACT CCCACCAGAG
CCAGGACCTC CACCAGCAAC TGTGCCCATG GCCCCCGGTC CCCCCAGTGT CTTCCCCCCT
GAGCCTGAGG AGCCAGAGGC AGACCAGCAC CAGCCCTTCC TTTTCCGCCA CGCCAGCTAC
TCATCTACCA CTTCGGATTG CGAGACTGAT GGCTACCTCA GCTCCTCCGG CTTCCTGGAT
GCCTCAGACC CTGCCCTTCA GCCCCCTGGG GGGGTGCCAT CCAGCCTGGC TGAGTCCCAT
CTCTGCCTGC CCTCGGCTTT TGCCCTATCC ATTCCACGTT CTGGCCCTGG AAGTGACTTT
TCCCCCGGGG ACAGCTATGC CTCAGATGCA GCTTCAGGCC TTAGCGATGT GGGAGAAGGG
ATGGGACAAA TGAGGAGACC CCCAGGGAGG AATCTCCGGC GCAGACCCCG ATCCCGGCTG
CGGGTCACTA GTGTCTCAGA CCAGAATGAC AGAGTGGTTG AGTGCCAGCT ACAGACCCAT
AACAGCAAGA TGGTGACCTT CCGATTTGAT CTGGATGGGG ACAGCCCGGA AGAGATTGCA
GCTGCCATGG TATATAACGA GTTCATTCTG CCTTCGGAGC GAGATGGATT TCTCAGACGG
ATTCGGGAGA TTATCCAGCG AGTGGAGACC CTGTTGAAGA GAGACACTGG CCCCATGGAG
GCTGCTGAAG ACACCCTAAG CCCCCAGGAG GAGCCAGCAC CATTACCTGC CCTGCCCGTC
CCCCTCCCAG ACCCATCCAA TGAAGAGCTC CAGAGCAGCA CCTCCCTGGA GCACAGGAGC
TGGACAGCCT TCTCCACCTC CTCATCTTCT CCTGGAACTC CTTTGTCTCC TGGAAACCCA
TTTTCCCCTG GAACCCCCAT TTCCCCAGGT CCCATCTTCC CCATCACTTC TCCCCCATGT
CATCCCAGCC CCTCCCCATT CTCCCCCATT TCTTCCCAGG TCTCCTCAAA TCCCTCTCCA
CACCCCACCA GCTCTCCACT TCCATTCTCC TCCAGCACAC CCGAGTTTCC GGTCCCACTC
TCTCAGTGTC CCTGGAGTTC TCTCCCCACG ACTTCTCCAC CTACGTTCTC TCCCACTTGT
TCTCAGGTCA CTCTTAGTTC CCCTTTCTTT CCTCCGTGCC CCTCCACTTC TTCCTTCCCC
TCCACCACAG CAGCCCCTCT CCTTTCTCTG GCTAGTGCCT TCTCACTGGC TGTGATGACT
GTGGCCCAGT CCCTGCTGTC CCCCTCACCT GGGCTCCTTT CCCAGTCTCC TCCAGCCCCT
CCTAGTCCCC TCCCTAGCCT GCCCCTTCCC CCTCCCGTTG CTCCTGGTGG CCAGGAAAGC
CCTTCACCCC ACACAGCTGA GGTGGAGAGT GAGGCCTCAC CACCTCCTGC TCGGCCCCTC
CCAGGGGAAG CCAGGCTGGC GCCCATCTCT GAAGAGGGAA AGCCGCAGCT TGTTGGGCGT
TTCCAAGTGA CTTCATCCAA GGAACCGGCT GAGCCTCTTC CCTTGCAGCC AACATCCCCC
ACTCTCTCTG GTTCTCCAAA ACCTTCAACC CCTCAGCTCA CTTCAGAGAG CTCAGATACA
GAGGACAGTG CTGGAGGCGG GCCAGAGACC AGGGAAGCTC TGGCTGAGAG CGACCGTGCA
GCTGAGGGTC TGGGGGCTGG AGTTGAGGAG GAAGGAGATG ATGGGAAGGA ACCCCAAGTT
GGGGGCAGCC CCCAACCCCT GAGCCATCCC AGCCCAGTGT GGATGAACTA CTCCTACAGC
AGCCTGTGTT TGAGCAGCGA GGAGTCAGAA AGCAGTGGGG AAGATGAGGA GTTCTGGGCT
GAGCTGCAGA GTCTTCGGCA GAAGCACTTG TCAGAGGTGG AAACACTACA GACACTACAG
AAAAAAGAAA TTGAAGATTT GTACAGCCGG CTGGGGAAGC AGCCCCCACC GGGTATTGTG
GCCCCAGCTG CTATGCTGTC CAGCCGCCAG CGCCGCCTCT CCAAGGGCAG CTTCCCCACC
TCCCGCCGCA ACAGCCTACA GCGCTCTGAG CCCCCAGGCC CTGGCATCAT GCGAAGGAAC
TCTCTGAGTG GCAGCAGCAC CGGCTCCCAG GAGCAGCGGG CAAGCAAGGG GGTGACATTC
GCCGGGGATG TTGGCAGGAT GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Src-family protein tyrosine kinase phosphorylates WNK4 and modulates its inhibitory effect on KCNJ1 (ROMK)
Proc. Natl. Acad. Sci. U.S.A. 112 (14), 4495-4500 (2015)
Lin DH, Yue P, Yarborough O 3rd, Scholl UI, Giebisch G, Lifton RP, Rinehart J and Wang WH.


book

Insights in cullin 3/WNK4 and its relationship to blood pressure regulation and electrolyte homeostasis
Cell. Signal. 26 (6), 1166-1172 (2014)
Anderica-Romero AC, Escobar L, Padilla-Flores T and Pedraza-Chaverri J.


book

WNK4 inhibits plasma membrane targeting of NCC through regulation of syntaxin13 SNARE formation
Cell. Signal. 25 (12), 2469-2477 (2013)
Chung WY, Park HW, Han JW, Lee MG and Kim JY.


book

Decrease of WNK4 ubiquitination by disease-causing mutations of KLHL3 through different molecular mechanisms
Biochem. Biophys. Res. Commun. 439 (1), 30-34 (2013)
Mori Y, Wakabayashi M, Mori T, Araki Y, Sohara E, Rai T, Sasaki S and Uchida S.


book

WNK4 regulates apical and basolateral Cl- flux in extrarenal epithelia
Proc. Natl. Acad. Sci. U.S.A. 101 (7), 2064-2069 (2004)
Kahle KT, Gimenez I, Hassan H, Wilson FH, Wong RD, Forbush B, Aronson PS and Lifton RP.


book

Molecular pathogenesis of inherited hypertension with hyperkalemia: the Na-Cl cotransporter is inhibited by wild-type but not mutant WNK4
Proc. Natl. Acad. Sci. U.S.A. 100 (2), 680-684 (2003)
Wilson FH, Kahle KT, Sabath E, Lalioti MD, Rapson AK, Hoover RS, Hebert SC, Gamba G and Lifton RP.


book

Expressed sequence tag analysis of human RPE/choroid for the NEIBank Project: over 6000 non-redundant transcripts, novel genes and splice variants
Mol. Vis. 8, 205-220 (2002)
Wistow G, Bernstein SL, Wyatt MK, Fariss RN, Behal A, Touchman JW, Bouffard G, Smith D and Peterson K.


book

WNK kinases, a novel protein kinase subfamily in multi-cellular organisms
Oncogene 20 (39), 5562-5569 (2001)
Verissimo F and Jordan P.


book

Human hypertension caused by mutations in WNK kinases
Science 293 (5532), 1107-1112 (2001)
Wilson FH, Disse-Nicodeme S, Choate KA, Ishikawa K, Nelson-Williams C, Desitter I, Gunel M, Milford DV, Lipkin GW, Achard JM, Feely MP, Dussol B, Berland Y, Unwin RJ, Mayan H, Simon DB, Farfel Z, Jeunemaitre X and Lifton RP.


book

Pseudohypoaldosteronism Type II
(in) Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Wilson,F.H. and Kahle,K.T.


 
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