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WNK4 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol WNK4
Entrez Gene ID 65266
Full Name WNK lysine deficient protein kinase 4
Synonyms PHA2B,PRKWNK4
General protein information
Preferred Names

WNK lysine deficient protein kinase 4

Names

serine/threonine-protein kinase WNK4
protein kinase lysine-deficient 4
protein kinase with no lysine 4

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

17

17q21.2

Summary This gene encodes a member of the WNK family of serine-threonine protein kinases. The kinase is part of the tight junction complex in kidney cells, and regulates the balance between NaCl reabsorption and K(+) secretion. The kinase regulates the activities of several types of ion channels, cotransporters, and exchangers involved in electrolyte flux in epithelial cells. Mutations in this gene result in pseudohypoaldosteronism type IIB.[provided by RefSeq, Sep 2009].
Disorder MIM:

601844

Disorder Html: Pseudohypoaldosteronism type II, 145260 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_032387.4 NP_115763.2 serine/threonine-protein kinase WNK4 isoform 1
NM_001321299.1 NP_001308228.1 serine/threonine-protein kinase WNK4 isoform 2
XM_017024962.1 XP_016880451.1 serine/threonine-protein kinase WNK4 isoform X1
XM_017024964.1 XP_016880453.1 serine/threonine-protein kinase WNK4 isoform X3
XM_011525135.2 XP_011523437.1 serine/threonine-protein kinase WNK4 isoform X5
XM_017024965.1 XP_016880454.1 serine/threonine-protein kinase WNK4 isoform X4
XM_017024963.1 XP_016880452.1 serine/threonine-protein kinase WNK4 isoform X2
XM_017024966.1 XP_016880455.1 serine/threonine-protein kinase WNK4 isoform X6



Gallus gallus (chicken) LOC777580 XP_003642875.2
Pan troglodytes (chimpanzee) WNK4 XP_003315535.1
Rattus norvegicus (Norway rat) Wnk4 NP_783169.2
Oryza sativa (rice) Os12g0162100 NP_001066222.1
Bos taurus (cattle) WNK4 XP_003587489.1
Mus musculus (house mouse) Wnk4 NP_783569.1
Oryza sativa (rice) Os11g0160300 NP_001065816.1
Homo sapiens (human) WNK4 NP_115763.2
Canis lupus familiaris (dog) WNK4 XP_548082.3
Macaca mulatta (rhesus monkey) LOC707820 XP_002808177.1


Related articles in PubMed

Impaired degradation of WNK by Akt and PKA phosphorylation of KLHL3.
Yoshizaki Y, Mori Y, Tsuzaki Y, Mori T, Nomura N, Wakabayashi M, Takahashi D, Zeniya M, Kikuchi E, Araki Y, Ando F, Isobe K, Nishida H, Ohta A, Susa K, Inoue Y, Chiga M, Rai T, Sasaki S, Uchida S, Sohara E
Biochemical and biophysical research communications467(2)229-34(2015 Nov)

Src-family protein tyrosine kinase phosphorylates WNK4 and modulates its inhibitory effect on KCNJ1 (ROMK).
Lin DH, Yue P, Yarborough O 3rd, Scholl UI, Giebisch G, Lifton RP, Rinehart J, Wang WH
Proceedings of the National Academy of Sciences of the United States of America112(14)4495-500(2015 Apr)

Insights in cullin 3/WNK4 and its relationship to blood pressure regulation and electrolyte homeostasis.
Andérica-Romero AC, Escobar L, Padilla-Flores T, Pedraza-Chaverri J
Cellular signalling26(6)1166-72(2014 Jun)

WNK4 inhibits plasma membrane targeting of NCC through regulation of syntaxin13 SNARE formation.
Chung WY, Park HW, Han JW, Lee MG, Kim JY
Cellular signalling25(12)2469-77(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study provides substantial new insights into the role of phosphorylation of KLHL3 in regulating the interaction with WNK4
Title: Phosphorylation of KLHL3 at serine 433 impairs its interaction with the acidic motif of WNK4: a molecular dynamics study.

The distribution of allele frequency and genotype of WNK4 gene Ala589Ser polymorphism showed significant differences between essential hypertension subjects, with or without type 2 diabetes mellitus, and normotensive subjects.
Title: Novel Association of WNK4 Gene, Ala589Ser Polymorphism in Essential Hypertension, and Type 2 Diabetes Mellitus in Malaysia.

Akt and PKA phosphorylated KLHL3 at S433, and phosphorylation of KLHL3 by PKA inhibited WNK4 degradation.
Title: Impaired degradation of WNK by Akt and PKA phosphorylation of KLHL3.

Data indicate that WNK lysine deficient protein kinase 4 protein (WNK4) was degraded not only by proteasomes but also by atypical protein kinase C scaffold protein p62 (p62)-kelch-like 3 protein (KLHL3)-mediated selective autophagy.
Title: Involvement of selective autophagy mediated by p62/SQSTM1 in KLHL3-dependent WNK4 degradation.

WNK4 is a substrate of SFKs and the association of c-Src and PTP-1D with WNK4 at Tyr(1092) and Tyr(1143) plays an important role in modulating the inhibitory effect of WNK4 on ROMK
Title: Src-family protein tyrosine kinase phosphorylates WNK4 and modulates its inhibitory effect on KCNJ1 (ROMK).

The following WNK4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WNK4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu50599 NM_001321299.1
Latest version!
Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu87050 XM_017024966.1
Latest version!
Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $475.30
$679.00
OHu87046 XM_017024962.1
Latest version!
Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
OHu87047 XM_017024963.1
Latest version!
Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
OHu87048 XM_017024964.1
Latest version!
Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
OHu87049 XM_017024965.1
Latest version!
Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
OHu17844 NM_032387.4
Latest version!
Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $384.30
$549.00
OHu71644 XM_011525135.2
Latest version!
Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $643.30
$919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu50599
Clone ID Related Accession (Same CDS sequence) NM_001321299.1
Accession Version NM_001321299.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2724bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-03-19
Organism Homo sapiens(Human)
Product serine/threonine-protein kinase WNK4 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CA388932.1, BC144356.1 and BM683764.1. On Mar 19, 2016 this sequence version replaced XM_006722022.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC144356.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142586 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

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ATGTCTTTAT CACGGGACCT ACTGGCTCTG TCAAAATCGG GGACCTGGGC CTGGCCACGC 
TCAAGCGCGC CTCCTTTGCC AAGAGTGTCA TCGGGACCCC GGAATTCATG GCCCCCGAGA
TGTACGAGGA AAAGTACGAT GAGGCCGTGG ACGTGTACGC GTTCGGCATG TGCATGCTGG
AGATGGCCAC CTCTGAGTAC CCGTACTCCG AGTGCCAGAA TGCCGCGCAA ATCTACCGCA
AGGTCACTTC GGTTCACCAT CCAGGACCTC CTGGCCCACG CCTTCTTCCG CGAGGAGCGC
GGTGTGCACG TGGAACTAGC GGAGGAGGAC GACGGCGAGA AGCCGGGCCT CAAGCTCTGG
CTGCGCATGG AGGACGCGCG GCGCGGGGGG CGCCCACGGG ACAACCAGGC CATCGAGTTC
CTGTTCCAGC TGGGCCGGGA CGCGGCCGAG GAGGTGGCAC AGGAGATGGT GGCTCTGGGC
TTGGTCTGTG AAGCCGATTA CCAGCCAGTG GCCCGTGCAG TACGTGAACG GGTTGCTGCC
ATCCAGCGAA AGCGTGAGAA GCTGCGTAAA GCAAGGGAAT TGGAGGCACT CCCACCAGAG
CCAGGACCTC CACCAGCAAC TGTGCCCATG GCCCCCGGTC CCCCCAGTGT CTTCCCCCCT
GAGCCTGAGG AGCCAGAGGC AGACCAGCAC CAGCCCTTCC TTTTCCGCCA CGCCAGCTAC
TCATCTACCA CTTCGGATTG CGAGACTGAT GGCTACCTCA GCTCCTCCGG CTTCCTGGAT
GCCTCAGACC CTGCCCTTCA GCCCCCTGGG GGGGTGCCAT CCAGCCTGGC TGAGTCCCAT
CTCTGCCTGC CCTCGGCTTT TGCCCTATCC ATTCCACGTT CTGGCCCTGG AAGTGACTTT
TCCCCCGGGG ACAGCTATGC CTCAGATGCA GCTTCAGGCC TTAGCGATGT GGGAGAAGGG
ATGGGACAAA TGAGGAGACC CCCAGGGAGG AATCTCCGGC GCAGACCCCG ATCCCGGCTG
CGGGTCACTA GTGTCTCAGA CCAGAATGAC AGAGTGGTTG AGTGCCAGCT ACAGACCCAT
AACAGCAAGA TGGTGACCTT CCGATTTGAT CTGGATGGGG ACAGCCCGGA AGAGATTGCA
GCTGCCATGG TATATAACGA GTTCATTCTG CCTTCGGAGC GAGATGGATT TCTCAGACGG
ATTCGGGAGA TTATCCAGCG AGTGGAGACC CTGTTGAAGA GAGACACTGG CCCCATGGAG
GCTGCTGAAG ACACCCTAAG CCCCCAGGAG GAGCCAGCAC CATTACCTGC CCTGCCCGTC
CCCCTCCCAG ACCCATCCAA TGAAGAGCTC CAGAGCAGCA CCTCCCTGGA GCACAGGAGC
TGGACAGCCT TCTCCACCTC CTCATCTTCT CCTGGAACTC CTTTGTCTCC TGGAAACCCA
TTTTCCCCTG GAACCCCCAT TTCCCCAGGT CCCATCTTCC CCATCACTTC TCCCCCATGT
CATCCCAGCC CCTCCCCATT CTCCCCCATT TCTTCCCAGG TCTCCTCAAA TCCCTCTCCA
CACCCCACCA GCTCTCCACT TCCATTCTCC TCCAGCACAC CCGAGTTTCC GGTCCCACTC
TCTCAGTGTC CCTGGAGTTC TCTCCCCACG ACTTCTCCAC CTACGTTCTC TCCCACTTGT
TCTCAGGTCA CTCTTAGTTC CCCTTTCTTT CCTCCGTGCC CCTCCACTTC TTCCTTCCCC
TCCACCACAG CAGCCCCTCT CCTTTCTCTG GCTAGTGCCT TCTCACTGGC TGTGATGACT
GTGGCCCAGT CCCTGCTGTC CCCCTCACCT GGGCTCCTTT CCCAGTCTCC TCCAGCCCCT
CCTAGTCCCC TCCCTAGCCT GCCCCTTCCC CCTCCCGTTG CTCCTGGTGG CCAGGAAAGC
CCTTCACCCC ACACAGCTGA GGTGGAGAGT GAGGCCTCAC CACCTCCTGC TCGGCCCCTC
CCAGGGGAAG CCAGGCTGGC GCCCATCTCT GAAGAGGGAA AGCCGCAGCT TGTTGGGCGT
TTCCAAGTGA CTTCATCCAA GGAACCGGCT GAGCCTCTTC CCTTGCAGCC AACATCCCCC
ACTCTCTCTG GTTCTCCAAA ACCTTCAACC CCTCAGCTCA CTTCAGAGAG CTCAGATACA
GAGGACAGTG CTGGAGGCGG GCCAGAGACC AGGGAAGCTC TGGCTGAGAG CGACCGTGCA
GCTGAGGGTC TGGGGGCTGG AGTTGAGGAG GAAGGAGATG ATGGGAAGGA ACCCCAAGTT
GGGGGCAGCC CCCAACCCCT GAGCCATCCC AGCCCAGTGT GGATGAACTA CTCCTACAGC
AGCCTGTGTT TGAGCAGCGA GGAGTCAGAA AGCAGTGGGG AAGATGAGGA GTTCTGGGCT
GAGCTGCAGA GTCTTCGGCA GAAGCACTTG TCAGAGGTGG AAACACTACA GACACTACAG
AAAAAAGAAA TTGAAGATTT GTACAGCCGG CTGGGGAAGC AGCCCCCACC GGGTATTGTG
GCCCCAGCTG CTATGCTGTC CAGCCGCCAG CGCCGCCTCT CCAAGGGCAG CTTCCCCACC
TCCCGCCGCA ACAGCCTACA GCGCTCTGAG CCCCCAGGCC CTGGCATCAT GCGAAGGAAC
TCTCTGAGTG GCAGCAGCAC CGGCTCCCAG GAGCAGCGGG CAAGCAAGGG GGTGACATTC
GCCGGGGATG TTGGCAGGAT GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001308228.1
CDS988..3711
Misc Feature(1)883..885
Misc Feature(2)1336..1446
Exon (1)1..686
Exon (2)687..859
Exon (3)860..1080
Exon (4)1081..1238
Exon (5)1239..1455
Exon (6)1456..1720
Exon (7)1721..1842
Exon (8)1843..1901
Exon (9)1902..2019
Exon (10)2020..2136
Exon (11)2137..2274
Exon (12)2275..2329
Exon (13)2330..2940
Exon (14)2941..3001
Exon (15)3002..3410
Exon (16)3411..3610
Exon (17)3611..3708
Exon (18)3709..4089
Translation

Target ORF information:

RefSeq Version NM_001321299.1
Organism Homo sapiens(Human)
Definition Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001321299.1

ORF Insert Sequence:

1
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121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGTCTTTAT CACGGGACCT ACTGGCTCTG TCAAAATCGG GGACCTGGGC CTGGCCACGC 
TCAAGCGCGC CTCCTTTGCC AAGAGTGTCA TCGGGACCCC GGAATTCATG GCCCCCGAGA
TGTACGAGGA AAAGTACGAT GAGGCCGTGG ACGTGTACGC GTTCGGCATG TGCATGCTGG
AGATGGCCAC CTCTGAGTAC CCGTACTCCG AGTGCCAGAA TGCCGCGCAA ATCTACCGCA
AGGTCACTTC GGTTCACCAT CCAGGACCTC CTGGCCCACG CCTTCTTCCG CGAGGAGCGC
GGTGTGCACG TGGAACTAGC GGAGGAGGAC GACGGCGAGA AGCCGGGCCT CAAGCTCTGG
CTGCGCATGG AGGACGCGCG GCGCGGGGGG CGCCCACGGG ACAACCAGGC CATCGAGTTC
CTGTTCCAGC TGGGCCGGGA CGCGGCCGAG GAGGTGGCAC AGGAGATGGT GGCTCTGGGC
TTGGTCTGTG AAGCCGATTA CCAGCCAGTG GCCCGTGCAG TACGTGAACG GGTTGCTGCC
ATCCAGCGAA AGCGTGAGAA GCTGCGTAAA GCAAGGGAAT TGGAGGCACT CCCACCAGAG
CCAGGACCTC CACCAGCAAC TGTGCCCATG GCCCCCGGTC CCCCCAGTGT CTTCCCCCCT
GAGCCTGAGG AGCCAGAGGC AGACCAGCAC CAGCCCTTCC TTTTCCGCCA CGCCAGCTAC
TCATCTACCA CTTCGGATTG CGAGACTGAT GGCTACCTCA GCTCCTCCGG CTTCCTGGAT
GCCTCAGACC CTGCCCTTCA GCCCCCTGGG GGGGTGCCAT CCAGCCTGGC TGAGTCCCAT
CTCTGCCTGC CCTCGGCTTT TGCCCTATCC ATTCCACGTT CTGGCCCTGG AAGTGACTTT
TCCCCCGGGG ACAGCTATGC CTCAGATGCA GCTTCAGGCC TTAGCGATGT GGGAGAAGGG
ATGGGACAAA TGAGGAGACC CCCAGGGAGG AATCTCCGGC GCAGACCCCG ATCCCGGCTG
CGGGTCACTA GTGTCTCAGA CCAGAATGAC AGAGTGGTTG AGTGCCAGCT ACAGACCCAT
AACAGCAAGA TGGTGACCTT CCGATTTGAT CTGGATGGGG ACAGCCCGGA AGAGATTGCA
GCTGCCATGG TATATAACGA GTTCATTCTG CCTTCGGAGC GAGATGGATT TCTCAGACGG
ATTCGGGAGA TTATCCAGCG AGTGGAGACC CTGTTGAAGA GAGACACTGG CCCCATGGAG
GCTGCTGAAG ACACCCTAAG CCCCCAGGAG GAGCCAGCAC CATTACCTGC CCTGCCCGTC
CCCCTCCCAG ACCCATCCAA TGAAGAGCTC CAGAGCAGCA CCTCCCTGGA GCACAGGAGC
TGGACAGCCT TCTCCACCTC CTCATCTTCT CCTGGAACTC CTTTGTCTCC TGGAAACCCA
TTTTCCCCTG GAACCCCCAT TTCCCCAGGT CCCATCTTCC CCATCACTTC TCCCCCATGT
CATCCCAGCC CCTCCCCATT CTCCCCCATT TCTTCCCAGG TCTCCTCAAA TCCCTCTCCA
CACCCCACCA GCTCTCCACT TCCATTCTCC TCCAGCACAC CCGAGTTTCC GGTCCCACTC
TCTCAGTGTC CCTGGAGTTC TCTCCCCACG ACTTCTCCAC CTACGTTCTC TCCCACTTGT
TCTCAGGTCA CTCTTAGTTC CCCTTTCTTT CCTCCGTGCC CCTCCACTTC TTCCTTCCCC
TCCACCACAG CAGCCCCTCT CCTTTCTCTG GCTAGTGCCT TCTCACTGGC TGTGATGACT
GTGGCCCAGT CCCTGCTGTC CCCCTCACCT GGGCTCCTTT CCCAGTCTCC TCCAGCCCCT
CCTAGTCCCC TCCCTAGCCT GCCCCTTCCC CCTCCCGTTG CTCCTGGTGG CCAGGAAAGC
CCTTCACCCC ACACAGCTGA GGTGGAGAGT GAGGCCTCAC CACCTCCTGC TCGGCCCCTC
CCAGGGGAAG CCAGGCTGGC GCCCATCTCT GAAGAGGGAA AGCCGCAGCT TGTTGGGCGT
TTCCAAGTGA CTTCATCCAA GGAACCGGCT GAGCCTCTTC CCTTGCAGCC AACATCCCCC
ACTCTCTCTG GTTCTCCAAA ACCTTCAACC CCTCAGCTCA CTTCAGAGAG CTCAGATACA
GAGGACAGTG CTGGAGGCGG GCCAGAGACC AGGGAAGCTC TGGCTGAGAG CGACCGTGCA
GCTGAGGGTC TGGGGGCTGG AGTTGAGGAG GAAGGAGATG ATGGGAAGGA ACCCCAAGTT
GGGGGCAGCC CCCAACCCCT GAGCCATCCC AGCCCAGTGT GGATGAACTA CTCCTACAGC
AGCCTGTGTT TGAGCAGCGA GGAGTCAGAA AGCAGTGGGG AAGATGAGGA GTTCTGGGCT
GAGCTGCAGA GTCTTCGGCA GAAGCACTTG TCAGAGGTGG AAACACTACA GACACTACAG
AAAAAAGAAA TTGAAGATTT GTACAGCCGG CTGGGGAAGC AGCCCCCACC GGGTATTGTG
GCCCCAGCTG CTATGCTGTC CAGCCGCCAG CGCCGCCTCT CCAAGGGCAG CTTCCCCACC
TCCCGCCGCA ACAGCCTACA GCGCTCTGAG CCCCCAGGCC CTGGCATCAT GCGAAGGAAC
TCTCTGAGTG GCAGCAGCAC CGGCTCCCAG GAGCAGCGGG CAAGCAAGGG GGTGACATTC
GCCGGGGATG TTGGCAGGAT GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu87050
Clone ID Related Accession (Same CDS sequence) XM_017024966.1
Accession Version XM_017024966.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2901bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product serine/threonine-protein kinase WNK4 isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGTCTTTAT CACGGGACCT ACTGGCTCTG TCAAAATCGG GGACCTGGGC CTGGCCACGC 
TCAAGCGCGC CTCCTTTGCC AAGAGTGTCA TCGGGACCCC GGAATTCATG GCCCCCGAGA
TGTACGAGGA AAAGTACGAT GAGGCCGTGG ACGTGTACGC GTTCGGCATG TGCATGCTGG
AGATGGCCAC CTCTGAGTAC CCGTACTCCG AGTGCCAGAA TGCCGCGCAA ATCTACCGCA
AGGTCACTTC GGTTCACCAT CCAGGACCTC CTGGCCCACG CCTTCTTCCG CGAGGAGCGC
GGTGTGCACG TGGAACTAGC GGAGGAGGAC GACGGCGAGA AGCCGGGCCT CAAGCTCTGG
CTGCGCATGG AGGACGCGCG GCGCGGGGGG CGCCCACGGG ACAACCAGGC CATCGAGTTC
CTGTTCCAGC TGGGCCGGGA CGCGGCCGAG GAGGTGGCAC AGGAGATGGT GGCTCTGGGC
TTGGTCTGTG AAGCCGATTA CCAGCCAGTG GCCCGTGCAG TACGTGAACG GGTTGCTGCC
ATCCAGCGAA AGCGTGAGAA GCTGCGTAAA GCAAGGGAAT TGGAGGCACT CCCACCAGAG
CCAGGACCTC CACCAGCAAC TGTGCCCATG GCCCCCGGTC CCCCCAGTGT CTTCCCCCCT
GAGCCTGAGG AGCCAGAGGC AGACCAGCAC CAGCCCTTCC TTTTCCGCCA CGCCAGCTAC
TCATCTACCA CTTCGGATTG CGAGACTGAT GGCTACCTCA GCTCCTCCGG CTTCCTGGAT
GCCTCAGACC CTGCCCTTCA GCCCCCTGGG GGGGTGCCAT CCAGCCTGGC TGAGTCCCAT
CTCTGCCTGC CCTCGGCTTT TGCCCTATCC ATTCCACGTT CTGGCCCTGG AAGTGACTTT
TCCCCCGGGG ACAGCTATGC CTCAGATGCA GCTTCAGGCC TTAGCGATGT GGGAGAAGGG
ATGGGACAAA TGAGGAGACC CCCAGGGAGG AATCTCCGGC GCAGACCCCG ATCCCGGCTG
CGGGTCACTA GTGTCTCAGA CCAGAATGAC AGAGTGGTTG AGTGCCAGCT ACAGACCCAT
AACAGCAAGA TGGTGACCTT CCGATTTGAT CTGGATGGGG ACAGCCCGGA AGAGATTGCA
GCTGCCATGG TATATAACGA GTTCATTCTG CCTTCGGAGC GAGATGGATT TCTCAGACGG
ATTCGGGAGA TTATCCAGCG AGTGGAGACC CTGTTGAAGA GAGACACTGG CCCCATGGAG
GCTGCTGAAG ACACCCTAAG CCCCCAGGAG GAGCCAGCAC CATTACCTGC CCTGCCCGTC
CCCCTCCCAG ACCCATCCAA TGAAGAGCTC CAGAGCAGCA CCTCCCTGGA GCACAGGAGC
TGGACAGCCT TCTCCACCTC CTCATCTTCT CCTGGAACTC CTTTGTCTCC TGGAAACCCA
TTTTCCCCTG GAACCCCCAT TTCCCCAGGT CCCATCTTCC CCATCACTTC TCCCCCATGT
CATCCCAGCC CCTCCCCATT CTCCCCCATT TCTTCCCAGG TCTCCTCAAA TCCCTCTCCA
CACCCCACCA GCTCTCCACT TCCATTCTCC TCCAGCACAC CCGAGTTTCC GGTCCCACTC
TCTCAGTGTC CCTGGAGTTC TCTCCCCACG ACTTCTCCAC CTACGTTCTC TCCCACTTGT
TCTCAGGTCA CTCTTAGTTC CCCTTTCTTT CCTCCGTGCC CCTCCACTTC TTCCTTCCCC
TCCACCACAG CAGCCCCTCT CCTTTCTCTG GCTAGTGCCT TCTCACTGGC TGTGATGACT
GTGGCCCAGT CCCTGCTGTC CCCCTCACCT GGGCTCCTTT CCCAGTCTCC TCCAGCCCCT
CCTAGTCCCC TCCCTAGCCT GCCCCTTCCC CCTCCCGTTG CTCCTGGTGG CCAGGAAAGC
CCTTCACCCC ACACAGCTGA GGTGGAGAGT GAGGCCTCAC CACCTCCTGC TCGGCCCCTC
CCAGGGGAAG CCAGGCTGGC GCCCATCTCT GAAGAGGGAA AGCCGCAGCT TGTTGGGCGT
TTCCAAGTGA CTTCATCCAA GGAACCGGCT GAGCCTCTTC CCTTGCAGCC AACATCCCCC
ACTCTCTCTG GTTCTCCAAA ACCTTCAACC CCTCAGCTCA CTTCAGAGAG CTCAGATACA
GAGGACAGTG CTGGAGGCGG GCCAGAGACC AGGGAAGCTC TGGCTGAGAG CGACCGTGCA
GCTGAGGGTC TGGGGGCTGG AGTTGAGGAG GAAGGAGATG ATGGGAAGGA ACCCCAAGTT
GGGGGCAGCC CCCAACCCCT GAGCCATCCC AGCCCAGTGT GGATGAACTA CTCCTACAGC
AGCCTGTGTT TGAGCAGCGA GGAGTCAGAA AGCAGTGGGG AAGATGAGGA GTTCTGGGCT
GAGCTGCAGA GTCTTCGGCA GAAGCACTTG TCAGAGGTGG AAACACTACA GACACTACAG
AAAAAAGAAA TTGAAGATTT GTACAGCCGG CTGGGGAAGC AGCCCCCACC GGGTATTGTG
GCCCCAGCTG CTATGCTGTC CAGCCGCCAG CGCCGCCTCT CCAAGGGCAG CTTCCCCACC
TCCCGCCGCA ACAGCCTACA GCGCTCTGAG CCCCCAGGCC CTGGCATCAT GCGAAGGAAC
TCTCTGAGTG GCAGCAGCAC CGGCTCCCAG GAGCAGCGGG CAAGCAAGGG GGTGACATTC
GCCGGGGATG TTGGCAGGAT GGTGAGGGCG GGCCCAAGGG AGGGAGAGCC CAGGGAATGG
TACCTGGCTG CAGCTTCGCC TTCCTCCCAC CTTGGAGGTT TCTTCATCAC TTTTTCTTTT
CCCTCCAGTG AATTCAGAAC AGAAGCCATG TATCTCCCCC ACACCAGGGC CCACCATGGA
GCTTGTGTTC TCAGAATCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016880455.1
CDS129..3029
Translation

Target ORF information:

RefSeq Version XM_017024966.1
Organism Homo sapiens(Human)
Definition Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017024966.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGTCTTTAT CACGGGACCT ACTGGCTCTG TCAAAATCGG GGACCTGGGC CTGGCCACGC 
TCAAGCGCGC CTCCTTTGCC AAGAGTGTCA TCGGGACCCC GGAATTCATG GCCCCCGAGA
TGTACGAGGA AAAGTACGAT GAGGCCGTGG ACGTGTACGC GTTCGGCATG TGCATGCTGG
AGATGGCCAC CTCTGAGTAC CCGTACTCCG AGTGCCAGAA TGCCGCGCAA ATCTACCGCA
AGGTCACTTC GGTTCACCAT CCAGGACCTC CTGGCCCACG CCTTCTTCCG CGAGGAGCGC
GGTGTGCACG TGGAACTAGC GGAGGAGGAC GACGGCGAGA AGCCGGGCCT CAAGCTCTGG
CTGCGCATGG AGGACGCGCG GCGCGGGGGG CGCCCACGGG ACAACCAGGC CATCGAGTTC
CTGTTCCAGC TGGGCCGGGA CGCGGCCGAG GAGGTGGCAC AGGAGATGGT GGCTCTGGGC
TTGGTCTGTG AAGCCGATTA CCAGCCAGTG GCCCGTGCAG TACGTGAACG GGTTGCTGCC
ATCCAGCGAA AGCGTGAGAA GCTGCGTAAA GCAAGGGAAT TGGAGGCACT CCCACCAGAG
CCAGGACCTC CACCAGCAAC TGTGCCCATG GCCCCCGGTC CCCCCAGTGT CTTCCCCCCT
GAGCCTGAGG AGCCAGAGGC AGACCAGCAC CAGCCCTTCC TTTTCCGCCA CGCCAGCTAC
TCATCTACCA CTTCGGATTG CGAGACTGAT GGCTACCTCA GCTCCTCCGG CTTCCTGGAT
GCCTCAGACC CTGCCCTTCA GCCCCCTGGG GGGGTGCCAT CCAGCCTGGC TGAGTCCCAT
CTCTGCCTGC CCTCGGCTTT TGCCCTATCC ATTCCACGTT CTGGCCCTGG AAGTGACTTT
TCCCCCGGGG ACAGCTATGC CTCAGATGCA GCTTCAGGCC TTAGCGATGT GGGAGAAGGG
ATGGGACAAA TGAGGAGACC CCCAGGGAGG AATCTCCGGC GCAGACCCCG ATCCCGGCTG
CGGGTCACTA GTGTCTCAGA CCAGAATGAC AGAGTGGTTG AGTGCCAGCT ACAGACCCAT
AACAGCAAGA TGGTGACCTT CCGATTTGAT CTGGATGGGG ACAGCCCGGA AGAGATTGCA
GCTGCCATGG TATATAACGA GTTCATTCTG CCTTCGGAGC GAGATGGATT TCTCAGACGG
ATTCGGGAGA TTATCCAGCG AGTGGAGACC CTGTTGAAGA GAGACACTGG CCCCATGGAG
GCTGCTGAAG ACACCCTAAG CCCCCAGGAG GAGCCAGCAC CATTACCTGC CCTGCCCGTC
CCCCTCCCAG ACCCATCCAA TGAAGAGCTC CAGAGCAGCA CCTCCCTGGA GCACAGGAGC
TGGACAGCCT TCTCCACCTC CTCATCTTCT CCTGGAACTC CTTTGTCTCC TGGAAACCCA
TTTTCCCCTG GAACCCCCAT TTCCCCAGGT CCCATCTTCC CCATCACTTC TCCCCCATGT
CATCCCAGCC CCTCCCCATT CTCCCCCATT TCTTCCCAGG TCTCCTCAAA TCCCTCTCCA
CACCCCACCA GCTCTCCACT TCCATTCTCC TCCAGCACAC CCGAGTTTCC GGTCCCACTC
TCTCAGTGTC CCTGGAGTTC TCTCCCCACG ACTTCTCCAC CTACGTTCTC TCCCACTTGT
TCTCAGGTCA CTCTTAGTTC CCCTTTCTTT CCTCCGTGCC CCTCCACTTC TTCCTTCCCC
TCCACCACAG CAGCCCCTCT CCTTTCTCTG GCTAGTGCCT TCTCACTGGC TGTGATGACT
GTGGCCCAGT CCCTGCTGTC CCCCTCACCT GGGCTCCTTT CCCAGTCTCC TCCAGCCCCT
CCTAGTCCCC TCCCTAGCCT GCCCCTTCCC CCTCCCGTTG CTCCTGGTGG CCAGGAAAGC
CCTTCACCCC ACACAGCTGA GGTGGAGAGT GAGGCCTCAC CACCTCCTGC TCGGCCCCTC
CCAGGGGAAG CCAGGCTGGC GCCCATCTCT GAAGAGGGAA AGCCGCAGCT TGTTGGGCGT
TTCCAAGTGA CTTCATCCAA GGAACCGGCT GAGCCTCTTC CCTTGCAGCC AACATCCCCC
ACTCTCTCTG GTTCTCCAAA ACCTTCAACC CCTCAGCTCA CTTCAGAGAG CTCAGATACA
GAGGACAGTG CTGGAGGCGG GCCAGAGACC AGGGAAGCTC TGGCTGAGAG CGACCGTGCA
GCTGAGGGTC TGGGGGCTGG AGTTGAGGAG GAAGGAGATG ATGGGAAGGA ACCCCAAGTT
GGGGGCAGCC CCCAACCCCT GAGCCATCCC AGCCCAGTGT GGATGAACTA CTCCTACAGC
AGCCTGTGTT TGAGCAGCGA GGAGTCAGAA AGCAGTGGGG AAGATGAGGA GTTCTGGGCT
GAGCTGCAGA GTCTTCGGCA GAAGCACTTG TCAGAGGTGG AAACACTACA GACACTACAG
AAAAAAGAAA TTGAAGATTT GTACAGCCGG CTGGGGAAGC AGCCCCCACC GGGTATTGTG
GCCCCAGCTG CTATGCTGTC CAGCCGCCAG CGCCGCCTCT CCAAGGGCAG CTTCCCCACC
TCCCGCCGCA ACAGCCTACA GCGCTCTGAG CCCCCAGGCC CTGGCATCAT GCGAAGGAAC
TCTCTGAGTG GCAGCAGCAC CGGCTCCCAG GAGCAGCGGG CAAGCAAGGG GGTGACATTC
GCCGGGGATG TTGGCAGGAT GGTGAGGGCG GGCCCAAGGG AGGGAGAGCC CAGGGAATGG
TACCTGGCTG CAGCTTCGCC TTCCTCCCAC CTTGGAGGTT TCTTCATCAC TTTTTCTTTT
CCCTCCAGTG AATTCAGAAC AGAAGCCATG TATCTCCCCC ACACCAGGGC CCACCATGGA
GCTTGTGTTC TCAGAATCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu87046
Clone ID Related Accession (Same CDS sequence) XM_017024962.1
Accession Version XM_017024962.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3909bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product serine/threonine-protein kinase WNK4 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
ATGTTGGCAT CCCCGGCCAC GGAGACCACC GTCCTCATGT CCCAGACTGA GGCCGACCTG 
GCCCTGCGGC CCCCGCCTCC TCTTGGCACC GCGGGGCAGC CCCGCCTCGG GCCCCCTCCT
CGCCGAGCGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC TCGTCTCAGC
CGCCGTAGCT CAGTCGACTT GGGGCTGCTG AGCTCTTGGT CCCTGCCAGC CTCACCCGCT
CCGGACCCCC CCGATCCTCC GGACTCCGCT GGTCCTGGCC CCGCGAGGAG CCCACCGCCT
AGCTCCAAAG AACCCCCCGA GGGCACGTGG ACCGAGGGAG CCCCTGTGAA GGCTGCGGAA
GACTCCGCGC GTCCCGAGCT CCCGGACTCT GCAGTGGGCC CGGGGTCCAG GGAGCCGCTA
AGGGTCCCTG AAGCTGTGGC CCTAGAGCGG CGGCGGGAGC AGGAAGAAAA GGAGGACATG
GAGACCCAGG CTGTGGCAAC GTCCCCCGAT GGCCGATACC TCAAGTTTGA CATCGAGATT
GGACGTGGCT CCTTCAAGAC GGTGTATCGA GGGCTAGACA CCGACACCAC AGTGGAGGTG
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTAGAGCTG AGCGGCAGCG CTTCTCAGAG
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA TGATTCGTGG
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
ACGCTCAAGA CGTACCTGAG GCGGTTCCGG GAGATGAAGC CGCGGGTCCT TCAGCGCTGG
AGCCGCCAAA TCCTGCGGGG ACTTCATTTC CTACACTCCC GGGTTCCTCC CATCCTGCAC
CGGGATCTCA AGTGCGACAA TGTCTTTATC ACGGGACCTA CTGGCTCTGT CAAAATCGGG
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTTGCCA AGAGTGTCAT CGGGACCCCG
GAATTCATGG CCCCCGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
TTCGGCATGT GCATGCTGGA GATGGCCACC TCTGAGTACC CGTACTCCGA GTGCCAGAAT
GCCGCGCAAA TCTACCGCAA GGTCACTTCG GGCAGAAAGC CGAACAGCTT CCACAAGGTG
AAGATACCCG AGGTGAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAACGAGAGG
TTCACCATCC AGGACCTCCT GGCCCACGCC TTCTTCCGCG AGGAGCGCGG TGTGCACGTG
GAACTAGCGG AGGAGGACGA CGGCGAGAAG CCGGGCCTCA AGCTCTGGCT GCGCATGGAG
GACGCGCGGC GCGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCCT GTTCCAGCTG
GGCCGGGACG CGGCCGAGGA GGTGGCACAG GAGATGGTGG CTCTGGGCTT GGTCTGTGAA
GCCGATTACC AGCCAGTGGC CCGTGCAGTA CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
CGTGAGAAGC TGCGTAAAGC AAGGGAATTG GAGGCACTCC CACCAGAGCC AGGACCTCCA
CCAGCAACTG TGCCCATGGC CCCCGGTCCC CCCAGTGTCT TCCCCCCTGA GCCTGAGGAG
CCAGAGGCAG ACCAGCACCA GCCCTTCCTT TTCCGCCACG CCAGCTACTC ATCTACCACT
TCGGATTGCG AGACTGATGG CTACCTCAGC TCCTCCGGCT TCCTGGATGC CTCAGACCCT
GCCCTTCAGC CCCCTGGGGG GGTGCCATCC AGCCTGGCTG AGTCCCATCT CTGCCTGCCC
TCGGCTTTTG CCCTATCCAT TCCACGTTCT GGCCCTGGAA GTGACTTTTC CCCCGGGGAC
AGCTATGCCT CAGATGCAGC TTCAGGCCTT AGCGATGTGG GAGAAGGGAT GGGACAAATG
AGGAGACCCC CAGGGAGGAA TCTCCGGCGC AGACCCCGAT CCCGGCTGCG GGTCACTAGT
GTCTCAGACC AGAATGACAG AGTGGTTGAG TGCCAGCTAC AGACCCATAA CAGCAAGATG
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCGGAAG AGATTGCAGC TGCCATGGTA
TATAACGAGT TCATTCTGCC TTCGGAGCGA GATGGATTTC TCAGACGGAT TCGGGAGATT
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GACACTGGCC CCATGGAGGC TGCTGAAGAC
ACCCTAAGCC CCCAGGAGGA GCCAGCACCA TTACCTGCCC TGCCCGTCCC CCTCCCAGAC
CCATCCAATG AAGAGCTCCA GAGCAGCACC TCCCTGGAGC ACAGGAGCTG GACAGCCTTC
TCCACCTCCT CATCTTCTCC TGGAACTCCT TTGTCTCCTG GAAACCCATT TTCCCCTGGA
ACCCCCATTT CCCCAGGTCC CATCTTCCCC ATCACTTCTC CCCCATGTCA TCCCAGCCCC
TCCCCATTCT CCCCCATTTC TTCCCAGGTC TCCTCAAATC CCTCTCCACA CCCCACCAGC
TCTCCACTTC CATTCTCCTC CAGCACACCC GAGTTTCCGG TCCCACTCTC TCAGTGTCCC
TGGAGTTCTC TCCCCACGAC TTCTCCACCT ACGTTCTCTC CCACTTGTTC TCAGGTCACT
CTTAGTTCCC CTTTCTTTCC TCCGTGCCCC TCCACTTCTT CCTTCCCCTC CACCACAGCA
GCCCCTCTCC TTTCTCTGGC TAGTGCCTTC TCACTGGCTG TGATGACTGT GGCCCAGTCC
CTGCTGTCCC CCTCACCTGG GCTCCTTTCC CAGTCTCCTC CAGCCCCTCC TAGTCCCCTC
CCTAGCCTGC CCCTTCCCCC TCCCGTTGCT CCTGGTGGCC AGGAAAGCCC TTCACCCCAC
ACAGCTGAGG TGGAGAGTGA GGCCTCACCA CCTCCTGCTC GGCCCCTCCC AGGGGAAGCC
AGGCTGGCGC CCATCTCTGA AGAGGGAAAG CCGCAGCTTG TTGGGCGTTT CCAAGTGACT
TCATCCAAGG AACCGGCTGA GCCTCTTCCC TTGCAGCCAA CATCCCCCAC TCTCTCTGGT
TCTCCAAAAC CTTCAACCCC TCAGCTCACT TCAGAGAGCT CAGATACAGA GGACAGTGCT
GGAGGCGGGC CAGAGACCAG GGAAGCTCTG GCTGAGAGCG ACCGTGCAGC TGAGGGTCTG
GGGGCTGGAG TTGAGGAGGA AGGAGATGAT GGGAAGGAAC CCCAAGTTGG GGGCAGCCCC
CAACCCCTGA GCCATCCCAG CCCAGTGTGG ATGAACTACT CCTACAGCAG CCTGTGTTTG
AGCAGCGAGG AGTCAGAAAG CAGTGGGGAA GATGAGGAGT TCTGGGCTGA GCTGCAGAGT
CTTCGGCAGA AGCACTTGTC AGAGGTGGAA ACACTACAGA CACTACAGAA AAAAGAAATT
GAAGATTTGT ACAGCCGGCT GGGGAAGCAG CCCCCACCGG GTATTGTGGC CCCAGCTGCT
ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC AAGGGCAGCT TCCCCACCTC CCGCCGCAAC
AGCCTACAGC GCTCTGAGCC CCCAGGCCCT GGCATCATGC GAAGGAACTC TCTGAGTGGC
AGCAGCACCG GCTCCCAGGA GCAGCGGGCA AGCAAGGGGG TGACATTCGC CGGGGATGTT
GGCAGGATGG TGAGGGCGGG CCCAAGGGAG GGAGAGCCCA GGGAATGGTA CCTGGCTGCA
GCTTCGCCTT CCTCCCACCT TGGAGGTTTC TTCATCACTT TTTCTTTTCC CTCCAGTGAA
TTCAGAACAG AAGCCATGTA TCTCCCCCAC ACCAGGGCCC ACCATGGAGC TTGTGTTCTC
AGAATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016880451.1
CDS999..4907
Translation

Target ORF information:

RefSeq Version XM_017024962.1
Organism Homo sapiens(Human)
Definition Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017024962.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
ATGTTGGCAT CCCCGGCCAC GGAGACCACC GTCCTCATGT CCCAGACTGA GGCCGACCTG 
GCCCTGCGGC CCCCGCCTCC TCTTGGCACC GCGGGGCAGC CCCGCCTCGG GCCCCCTCCT
CGCCGAGCGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC TCGTCTCAGC
CGCCGTAGCT CAGTCGACTT GGGGCTGCTG AGCTCTTGGT CCCTGCCAGC CTCACCCGCT
CCGGACCCCC CCGATCCTCC GGACTCCGCT GGTCCTGGCC CCGCGAGGAG CCCACCGCCT
AGCTCCAAAG AACCCCCCGA GGGCACGTGG ACCGAGGGAG CCCCTGTGAA GGCTGCGGAA
GACTCCGCGC GTCCCGAGCT CCCGGACTCT GCAGTGGGCC CGGGGTCCAG GGAGCCGCTA
AGGGTCCCTG AAGCTGTGGC CCTAGAGCGG CGGCGGGAGC AGGAAGAAAA GGAGGACATG
GAGACCCAGG CTGTGGCAAC GTCCCCCGAT GGCCGATACC TCAAGTTTGA CATCGAGATT
GGACGTGGCT CCTTCAAGAC GGTGTATCGA GGGCTAGACA CCGACACCAC AGTGGAGGTG
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTAGAGCTG AGCGGCAGCG CTTCTCAGAG
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA TGATTCGTGG
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
ACGCTCAAGA CGTACCTGAG GCGGTTCCGG GAGATGAAGC CGCGGGTCCT TCAGCGCTGG
AGCCGCCAAA TCCTGCGGGG ACTTCATTTC CTACACTCCC GGGTTCCTCC CATCCTGCAC
CGGGATCTCA AGTGCGACAA TGTCTTTATC ACGGGACCTA CTGGCTCTGT CAAAATCGGG
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTTGCCA AGAGTGTCAT CGGGACCCCG
GAATTCATGG CCCCCGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
TTCGGCATGT GCATGCTGGA GATGGCCACC TCTGAGTACC CGTACTCCGA GTGCCAGAAT
GCCGCGCAAA TCTACCGCAA GGTCACTTCG GGCAGAAAGC CGAACAGCTT CCACAAGGTG
AAGATACCCG AGGTGAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAACGAGAGG
TTCACCATCC AGGACCTCCT GGCCCACGCC TTCTTCCGCG AGGAGCGCGG TGTGCACGTG
GAACTAGCGG AGGAGGACGA CGGCGAGAAG CCGGGCCTCA AGCTCTGGCT GCGCATGGAG
GACGCGCGGC GCGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCCT GTTCCAGCTG
GGCCGGGACG CGGCCGAGGA GGTGGCACAG GAGATGGTGG CTCTGGGCTT GGTCTGTGAA
GCCGATTACC AGCCAGTGGC CCGTGCAGTA CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
CGTGAGAAGC TGCGTAAAGC AAGGGAATTG GAGGCACTCC CACCAGAGCC AGGACCTCCA
CCAGCAACTG TGCCCATGGC CCCCGGTCCC CCCAGTGTCT TCCCCCCTGA GCCTGAGGAG
CCAGAGGCAG ACCAGCACCA GCCCTTCCTT TTCCGCCACG CCAGCTACTC ATCTACCACT
TCGGATTGCG AGACTGATGG CTACCTCAGC TCCTCCGGCT TCCTGGATGC CTCAGACCCT
GCCCTTCAGC CCCCTGGGGG GGTGCCATCC AGCCTGGCTG AGTCCCATCT CTGCCTGCCC
TCGGCTTTTG CCCTATCCAT TCCACGTTCT GGCCCTGGAA GTGACTTTTC CCCCGGGGAC
AGCTATGCCT CAGATGCAGC TTCAGGCCTT AGCGATGTGG GAGAAGGGAT GGGACAAATG
AGGAGACCCC CAGGGAGGAA TCTCCGGCGC AGACCCCGAT CCCGGCTGCG GGTCACTAGT
GTCTCAGACC AGAATGACAG AGTGGTTGAG TGCCAGCTAC AGACCCATAA CAGCAAGATG
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCGGAAG AGATTGCAGC TGCCATGGTA
TATAACGAGT TCATTCTGCC TTCGGAGCGA GATGGATTTC TCAGACGGAT TCGGGAGATT
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GACACTGGCC CCATGGAGGC TGCTGAAGAC
ACCCTAAGCC CCCAGGAGGA GCCAGCACCA TTACCTGCCC TGCCCGTCCC CCTCCCAGAC
CCATCCAATG AAGAGCTCCA GAGCAGCACC TCCCTGGAGC ACAGGAGCTG GACAGCCTTC
TCCACCTCCT CATCTTCTCC TGGAACTCCT TTGTCTCCTG GAAACCCATT TTCCCCTGGA
ACCCCCATTT CCCCAGGTCC CATCTTCCCC ATCACTTCTC CCCCATGTCA TCCCAGCCCC
TCCCCATTCT CCCCCATTTC TTCCCAGGTC TCCTCAAATC CCTCTCCACA CCCCACCAGC
TCTCCACTTC CATTCTCCTC CAGCACACCC GAGTTTCCGG TCCCACTCTC TCAGTGTCCC
TGGAGTTCTC TCCCCACGAC TTCTCCACCT ACGTTCTCTC CCACTTGTTC TCAGGTCACT
CTTAGTTCCC CTTTCTTTCC TCCGTGCCCC TCCACTTCTT CCTTCCCCTC CACCACAGCA
GCCCCTCTCC TTTCTCTGGC TAGTGCCTTC TCACTGGCTG TGATGACTGT GGCCCAGTCC
CTGCTGTCCC CCTCACCTGG GCTCCTTTCC CAGTCTCCTC CAGCCCCTCC TAGTCCCCTC
CCTAGCCTGC CCCTTCCCCC TCCCGTTGCT CCTGGTGGCC AGGAAAGCCC TTCACCCCAC
ACAGCTGAGG TGGAGAGTGA GGCCTCACCA CCTCCTGCTC GGCCCCTCCC AGGGGAAGCC
AGGCTGGCGC CCATCTCTGA AGAGGGAAAG CCGCAGCTTG TTGGGCGTTT CCAAGTGACT
TCATCCAAGG AACCGGCTGA GCCTCTTCCC TTGCAGCCAA CATCCCCCAC TCTCTCTGGT
TCTCCAAAAC CTTCAACCCC TCAGCTCACT TCAGAGAGCT CAGATACAGA GGACAGTGCT
GGAGGCGGGC CAGAGACCAG GGAAGCTCTG GCTGAGAGCG ACCGTGCAGC TGAGGGTCTG
GGGGCTGGAG TTGAGGAGGA AGGAGATGAT GGGAAGGAAC CCCAAGTTGG GGGCAGCCCC
CAACCCCTGA GCCATCCCAG CCCAGTGTGG ATGAACTACT CCTACAGCAG CCTGTGTTTG
AGCAGCGAGG AGTCAGAAAG CAGTGGGGAA GATGAGGAGT TCTGGGCTGA GCTGCAGAGT
CTTCGGCAGA AGCACTTGTC AGAGGTGGAA ACACTACAGA CACTACAGAA AAAAGAAATT
GAAGATTTGT ACAGCCGGCT GGGGAAGCAG CCCCCACCGG GTATTGTGGC CCCAGCTGCT
ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC AAGGGCAGCT TCCCCACCTC CCGCCGCAAC
AGCCTACAGC GCTCTGAGCC CCCAGGCCCT GGCATCATGC GAAGGAACTC TCTGAGTGGC
AGCAGCACCG GCTCCCAGGA GCAGCGGGCA AGCAAGGGGG TGACATTCGC CGGGGATGTT
GGCAGGATGG TGAGGGCGGG CCCAAGGGAG GGAGAGCCCA GGGAATGGTA CCTGGCTGCA
GCTTCGCCTT CCTCCCACCT TGGAGGTTTC TTCATCACTT TTTCTTTTCC CTCCAGTGAA
TTCAGAACAG AAGCCATGTA TCTCCCCCAC ACCAGGGCCC ACCATGGAGC TTGTGTTCTC
AGAATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu87047
Clone ID Related Accession (Same CDS sequence) XM_017024963.1
Accession Version XM_017024963.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3906bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product serine/threonine-protein kinase WNK4 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
ATGTTGGCAT CCCCGGCCAC GGAGACCACC GTCCTCATGT CCCAGACTGA GGCCGACCTG 
GCCCTGCGGC CCCCGCCTCC TCTTGGCACC GCGGGGCAGC CCCGCCTCGG GCCCCCTCCT
CGCCGAGCGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC TCGTCTCAGC
CGCCGTAGCT CAGTCGACTT GGGGCTGCTG AGCTCTTGGT CCCTGCCAGC CTCACCCGCT
CCGGACCCCC CCGATCCTCC GGACTCCGCT GGTCCTGGCC CCGCGAGGAG CCCACCGCCT
AGCTCCAAAG AACCCCCCGA GGGCACGTGG ACCGAGGGAG CCCCTGTGAA GGCTGCGGAA
GACTCCGCGC GTCCCGAGCT CCCGGACTCT GCAGTGGGCC CGGGGTCCAG GGAGCCGCTA
AGGGTCCCTG AAGCTGTGGC CCTAGAGCGG CGGCGGGAGC AGGAAGAAAA GGAGGACATG
GAGACCCAGG CTGTGGCAAC GTCCCCCGAT GGCCGATACC TCAAGTTTGA CATCGAGATT
GGACGTGGCT CCTTCAAGAC GGTGTATCGA GGGCTAGACA CCGACACCAC AGTGGAGGTG
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTAGAGCTG AGCGGCAGCG CTTCTCAGAG
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA TGATTCGTGG
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
ACGCTCAAGA CGTACCTGAG GCGGTTCCGG GAGATGAAGC CGCGGGTCCT TCAGCGCTGG
AGCCGCCAAA TCCTGCGGGG ACTTCATTTC CTACACTCCC GGGTTCCTCC CATCCTGCAC
CGGGATCTCA AGTGCGACAA TGTCTTTATC ACGGGACCTA CTGGCTCTGT CAAAATCGGG
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTTGCCA AGAGTGTCAT CGGGACCCCG
GAATTCATGG CCCCCGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
TTCGGCATGT GCATGCTGGA GATGGCCACC TCTGAGTACC CGTACTCCGA GTGCCAGAAT
GCCGCGCAAA TCTACCGCAA GGTCACTTCG GGCAGAAAGC CGAACAGCTT CCACAAGGTG
AAGATACCCG AGGTGAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAACGAGAGG
TTCACCATCC AGGACCTCCT GGCCCACGCC TTCTTCCGCG AGGAGCGCGG TGTGCACGTG
GAACTAGCGG AGGAGGACGA CGGCGAGAAG CCGGGCCTCA AGCTCTGGCT GCGCATGGAG
GACGCGCGGC GCGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCCT GTTCCAGCTG
GGCCGGGACG CGGCCGAGGA GGTGGCACAG GAGATGGTGG CTCTGGGCTT GGTCTGTGAA
GCCGATTACC AGCCAGTGGC CCGTGCAGTA CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
CGTGAGAAGC TGCGTAAAGC AAGGGAATTG GAGGCACTCC CACCAGAGCC AGGACCTCCA
CCAGCAACTG TGCCCATGGC CCCCGGTCCC CCCAGTGTCT TCCCCCCTGA GCCTGAGGAG
CCAGAGGCAG ACCAGCACCA GCCCTTCCTT TTCCGCCACG CCAGCTACTC ATCTACCACT
TCGGATTGCG AGACTGATGG CTACCTCAGC TCCTCCGGCT TCCTGGATGC CTCAGACCCT
GCCCTTCAGC CCCCTGGGGG GGTGCCATCC AGCCTGGCTG AGTCCCATCT CTGCCTGCCC
TCGGCTTTTG CCCTATCCAT TCCACGTTCT GGCCCTGGAA GTGACTTTTC CCCCGGGGAC
AGCTATGCCT CAGATGCAGC TTCAGGCCTT AGCGATGTGG GAGAAGGGAT GGGACAAATG
AGGAGACCCC CAGGGAGGAA TCTCCGGCGC AGACCCCGAT CCCGGCTGCG GGTCACTAGT
GTCTCAGACC AGAATGACAG AGTGGTTGAG TGCCAGCTAC AGACCCATAA CAGCAAGATG
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCGGAAG AGATTGCAGC TGCCATGGTA
TATAACGAGT TCATTCTGCC TTCGGAGCGA GATGGATTTC TCAGACGGAT TCGGGAGATT
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GACACTGGCC CCATGGAGGC TGCTGAAGAC
ACCCTAAGCC CCCAGGAGGA GCCAGCACCA TTACCTGCCC TGCCCGTCCC CCTCCCAGAC
CCATCCAATG AGCTCCAGAG CAGCACCTCC CTGGAGCACA GGAGCTGGAC AGCCTTCTCC
ACCTCCTCAT CTTCTCCTGG AACTCCTTTG TCTCCTGGAA ACCCATTTTC CCCTGGAACC
CCCATTTCCC CAGGTCCCAT CTTCCCCATC ACTTCTCCCC CATGTCATCC CAGCCCCTCC
CCATTCTCCC CCATTTCTTC CCAGGTCTCC TCAAATCCCT CTCCACACCC CACCAGCTCT
CCACTTCCAT TCTCCTCCAG CACACCCGAG TTTCCGGTCC CACTCTCTCA GTGTCCCTGG
AGTTCTCTCC CCACGACTTC TCCACCTACG TTCTCTCCCA CTTGTTCTCA GGTCACTCTT
AGTTCCCCTT TCTTTCCTCC GTGCCCCTCC ACTTCTTCCT TCCCCTCCAC CACAGCAGCC
CCTCTCCTTT CTCTGGCTAG TGCCTTCTCA CTGGCTGTGA TGACTGTGGC CCAGTCCCTG
CTGTCCCCCT CACCTGGGCT CCTTTCCCAG TCTCCTCCAG CCCCTCCTAG TCCCCTCCCT
AGCCTGCCCC TTCCCCCTCC CGTTGCTCCT GGTGGCCAGG AAAGCCCTTC ACCCCACACA
GCTGAGGTGG AGAGTGAGGC CTCACCACCT CCTGCTCGGC CCCTCCCAGG GGAAGCCAGG
CTGGCGCCCA TCTCTGAAGA GGGAAAGCCG CAGCTTGTTG GGCGTTTCCA AGTGACTTCA
TCCAAGGAAC CGGCTGAGCC TCTTCCCTTG CAGCCAACAT CCCCCACTCT CTCTGGTTCT
CCAAAACCTT CAACCCCTCA GCTCACTTCA GAGAGCTCAG ATACAGAGGA CAGTGCTGGA
GGCGGGCCAG AGACCAGGGA AGCTCTGGCT GAGAGCGACC GTGCAGCTGA GGGTCTGGGG
GCTGGAGTTG AGGAGGAAGG AGATGATGGG AAGGAACCCC AAGTTGGGGG CAGCCCCCAA
CCCCTGAGCC ATCCCAGCCC AGTGTGGATG AACTACTCCT ACAGCAGCCT GTGTTTGAGC
AGCGAGGAGT CAGAAAGCAG TGGGGAAGAT GAGGAGTTCT GGGCTGAGCT GCAGAGTCTT
CGGCAGAAGC ACTTGTCAGA GGTGGAAACA CTACAGACAC TACAGAAAAA AGAAATTGAA
GATTTGTACA GCCGGCTGGG GAAGCAGCCC CCACCGGGTA TTGTGGCCCC AGCTGCTATG
CTGTCCAGCC GCCAGCGCCG CCTCTCCAAG GGCAGCTTCC CCACCTCCCG CCGCAACAGC
CTACAGCGCT CTGAGCCCCC AGGCCCTGGC ATCATGCGAA GGAACTCTCT GAGTGGCAGC
AGCACCGGCT CCCAGGAGCA GCGGGCAAGC AAGGGGGTGA CATTCGCCGG GGATGTTGGC
AGGATGGTGA GGGCGGGCCC AAGGGAGGGA GAGCCCAGGG AATGGTACCT GGCTGCAGCT
TCGCCTTCCT CCCACCTTGG AGGTTTCTTC ATCACTTTTT CTTTTCCCTC CAGTGAATTC
AGAACAGAAG CCATGTATCT CCCCCACACC AGGGCCCACC ATGGAGCTTG TGTTCTCAGA
ATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016880452.1
CDS999..4904
Translation

Target ORF information:

RefSeq Version XM_017024963.1
Organism Homo sapiens(Human)
Definition Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017024963.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
ATGTTGGCAT CCCCGGCCAC GGAGACCACC GTCCTCATGT CCCAGACTGA GGCCGACCTG 
GCCCTGCGGC CCCCGCCTCC TCTTGGCACC GCGGGGCAGC CCCGCCTCGG GCCCCCTCCT
CGCCGAGCGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC TCGTCTCAGC
CGCCGTAGCT CAGTCGACTT GGGGCTGCTG AGCTCTTGGT CCCTGCCAGC CTCACCCGCT
CCGGACCCCC CCGATCCTCC GGACTCCGCT GGTCCTGGCC CCGCGAGGAG CCCACCGCCT
AGCTCCAAAG AACCCCCCGA GGGCACGTGG ACCGAGGGAG CCCCTGTGAA GGCTGCGGAA
GACTCCGCGC GTCCCGAGCT CCCGGACTCT GCAGTGGGCC CGGGGTCCAG GGAGCCGCTA
AGGGTCCCTG AAGCTGTGGC CCTAGAGCGG CGGCGGGAGC AGGAAGAAAA GGAGGACATG
GAGACCCAGG CTGTGGCAAC GTCCCCCGAT GGCCGATACC TCAAGTTTGA CATCGAGATT
GGACGTGGCT CCTTCAAGAC GGTGTATCGA GGGCTAGACA CCGACACCAC AGTGGAGGTG
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTAGAGCTG AGCGGCAGCG CTTCTCAGAG
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA TGATTCGTGG
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
ACGCTCAAGA CGTACCTGAG GCGGTTCCGG GAGATGAAGC CGCGGGTCCT TCAGCGCTGG
AGCCGCCAAA TCCTGCGGGG ACTTCATTTC CTACACTCCC GGGTTCCTCC CATCCTGCAC
CGGGATCTCA AGTGCGACAA TGTCTTTATC ACGGGACCTA CTGGCTCTGT CAAAATCGGG
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTTGCCA AGAGTGTCAT CGGGACCCCG
GAATTCATGG CCCCCGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
TTCGGCATGT GCATGCTGGA GATGGCCACC TCTGAGTACC CGTACTCCGA GTGCCAGAAT
GCCGCGCAAA TCTACCGCAA GGTCACTTCG GGCAGAAAGC CGAACAGCTT CCACAAGGTG
AAGATACCCG AGGTGAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAACGAGAGG
TTCACCATCC AGGACCTCCT GGCCCACGCC TTCTTCCGCG AGGAGCGCGG TGTGCACGTG
GAACTAGCGG AGGAGGACGA CGGCGAGAAG CCGGGCCTCA AGCTCTGGCT GCGCATGGAG
GACGCGCGGC GCGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCCT GTTCCAGCTG
GGCCGGGACG CGGCCGAGGA GGTGGCACAG GAGATGGTGG CTCTGGGCTT GGTCTGTGAA
GCCGATTACC AGCCAGTGGC CCGTGCAGTA CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
CGTGAGAAGC TGCGTAAAGC AAGGGAATTG GAGGCACTCC CACCAGAGCC AGGACCTCCA
CCAGCAACTG TGCCCATGGC CCCCGGTCCC CCCAGTGTCT TCCCCCCTGA GCCTGAGGAG
CCAGAGGCAG ACCAGCACCA GCCCTTCCTT TTCCGCCACG CCAGCTACTC ATCTACCACT
TCGGATTGCG AGACTGATGG CTACCTCAGC TCCTCCGGCT TCCTGGATGC CTCAGACCCT
GCCCTTCAGC CCCCTGGGGG GGTGCCATCC AGCCTGGCTG AGTCCCATCT CTGCCTGCCC
TCGGCTTTTG CCCTATCCAT TCCACGTTCT GGCCCTGGAA GTGACTTTTC CCCCGGGGAC
AGCTATGCCT CAGATGCAGC TTCAGGCCTT AGCGATGTGG GAGAAGGGAT GGGACAAATG
AGGAGACCCC CAGGGAGGAA TCTCCGGCGC AGACCCCGAT CCCGGCTGCG GGTCACTAGT
GTCTCAGACC AGAATGACAG AGTGGTTGAG TGCCAGCTAC AGACCCATAA CAGCAAGATG
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCGGAAG AGATTGCAGC TGCCATGGTA
TATAACGAGT TCATTCTGCC TTCGGAGCGA GATGGATTTC TCAGACGGAT TCGGGAGATT
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GACACTGGCC CCATGGAGGC TGCTGAAGAC
ACCCTAAGCC CCCAGGAGGA GCCAGCACCA TTACCTGCCC TGCCCGTCCC CCTCCCAGAC
CCATCCAATG AGCTCCAGAG CAGCACCTCC CTGGAGCACA GGAGCTGGAC AGCCTTCTCC
ACCTCCTCAT CTTCTCCTGG AACTCCTTTG TCTCCTGGAA ACCCATTTTC CCCTGGAACC
CCCATTTCCC CAGGTCCCAT CTTCCCCATC ACTTCTCCCC CATGTCATCC CAGCCCCTCC
CCATTCTCCC CCATTTCTTC CCAGGTCTCC TCAAATCCCT CTCCACACCC CACCAGCTCT
CCACTTCCAT TCTCCTCCAG CACACCCGAG TTTCCGGTCC CACTCTCTCA GTGTCCCTGG
AGTTCTCTCC CCACGACTTC TCCACCTACG TTCTCTCCCA CTTGTTCTCA GGTCACTCTT
AGTTCCCCTT TCTTTCCTCC GTGCCCCTCC ACTTCTTCCT TCCCCTCCAC CACAGCAGCC
CCTCTCCTTT CTCTGGCTAG TGCCTTCTCA CTGGCTGTGA TGACTGTGGC CCAGTCCCTG
CTGTCCCCCT CACCTGGGCT CCTTTCCCAG TCTCCTCCAG CCCCTCCTAG TCCCCTCCCT
AGCCTGCCCC TTCCCCCTCC CGTTGCTCCT GGTGGCCAGG AAAGCCCTTC ACCCCACACA
GCTGAGGTGG AGAGTGAGGC CTCACCACCT CCTGCTCGGC CCCTCCCAGG GGAAGCCAGG
CTGGCGCCCA TCTCTGAAGA GGGAAAGCCG CAGCTTGTTG GGCGTTTCCA AGTGACTTCA
TCCAAGGAAC CGGCTGAGCC TCTTCCCTTG CAGCCAACAT CCCCCACTCT CTCTGGTTCT
CCAAAACCTT CAACCCCTCA GCTCACTTCA GAGAGCTCAG ATACAGAGGA CAGTGCTGGA
GGCGGGCCAG AGACCAGGGA AGCTCTGGCT GAGAGCGACC GTGCAGCTGA GGGTCTGGGG
GCTGGAGTTG AGGAGGAAGG AGATGATGGG AAGGAACCCC AAGTTGGGGG CAGCCCCCAA
CCCCTGAGCC ATCCCAGCCC AGTGTGGATG AACTACTCCT ACAGCAGCCT GTGTTTGAGC
AGCGAGGAGT CAGAAAGCAG TGGGGAAGAT GAGGAGTTCT GGGCTGAGCT GCAGAGTCTT
CGGCAGAAGC ACTTGTCAGA GGTGGAAACA CTACAGACAC TACAGAAAAA AGAAATTGAA
GATTTGTACA GCCGGCTGGG GAAGCAGCCC CCACCGGGTA TTGTGGCCCC AGCTGCTATG
CTGTCCAGCC GCCAGCGCCG CCTCTCCAAG GGCAGCTTCC CCACCTCCCG CCGCAACAGC
CTACAGCGCT CTGAGCCCCC AGGCCCTGGC ATCATGCGAA GGAACTCTCT GAGTGGCAGC
AGCACCGGCT CCCAGGAGCA GCGGGCAAGC AAGGGGGTGA CATTCGCCGG GGATGTTGGC
AGGATGGTGA GGGCGGGCCC AAGGGAGGGA GAGCCCAGGG AATGGTACCT GGCTGCAGCT
TCGCCTTCCT CCCACCTTGG AGGTTTCTTC ATCACTTTTT CTTTTCCCTC CAGTGAATTC
AGAACAGAAG CCATGTATCT CCCCCACACC AGGGCCCACC ATGGAGCTTG TGTTCTCAGA
ATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu87048
Clone ID Related Accession (Same CDS sequence) XM_017024964.1
Accession Version XM_017024964.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3771bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product serine/threonine-protein kinase WNK4 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGTTGGCAT CCCCGGCCAC GGAGACCACC GTCCTCATGT CCCAGACTGA GGCCGACCTG 
GCCCTGCGGC CCCCGCCTCC TCTTGGCACC GCGGGGCAGC CCCGCCTCGG GCCCCCTCCT
CGCCGAGCGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC TCGTCTCAGC
CGCCGTAGCT CAGTCGACTT GGGGCTGCTG AGCTCTTGGT CCCTGCCAGC CTCACCCGCT
CCGGACCCCC CCGATCCTCC GGACTCCGCT GGTCCTGGCC CCGCGAGGAG CCCACCGCCT
AGCTCCAAAG AACCCCCCGA GGGCACGTGG ACCGAGGGAG CCCCTGTGAA GGCTGCGGAA
GACTCCGCGC GTCCCGAGCT CCCGGACTCT GCAGTGGGCC CGGGGTCCAG GGAGCCGCTA
AGGGTCCCTG AAGCTGTGGC CCTAGAGCGG CGGCGGGAGC AGGAAGAAAA GGAGGACATG
GAGACCCAGG CTGTGGCAAC GTCCCCCGAT GGCCGATACC TCAAGTTTGA CATCGAGATT
GGACGTGGCT CCTTCAAGAC GGTGTATCGA GGGCTAGACA CCGACACCAC AGTGGAGGTG
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTAGAGCTG AGCGGCAGCG CTTCTCAGAG
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA TGATTCGTGG
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
ACGCTCAAGA CGTACCTGAG GCGGTTCCGG GAGATGAAGC CGCGGGTCCT TCAGCGCTGG
AGCCGCCAAA TCCTGCGGGG ACTTCATTTC CTACACTCCC GGGTTCCTCC CATCCTGCAC
CGGGATCTCA AGTGCGACAA TGTCTTTATC ACGGGACCTA CTGGCTCTGT CAAAATCGGG
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTTGCCA AGAGTGTCAT CGGGACCCCG
GAATTCATGG CCCCCGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
TTCGGCATGT GCATGCTGGA GATGGCCACC TCTGAGTACC CGTACTCCGA GTGCCAGAAT
GCCGCGCAAA TCTACCGCAA GGTCACTTCG GGCAGAAAGC CGAACAGCTT CCACAAGGTG
AAGATACCCG AGGTGAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAACGAGAGG
TTCACCATCC AGGACCTCCT GGCCCACGCC TTCTTCCGCG AGGAGCGCGG TGTGCACGTG
GAACTAGCGG AGGAGGACGA CGGCGAGAAG CCGGGCCTCA AGCTCTGGCT GCGCATGGAG
GACGCGCGGC GCGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCCT GTTCCAGCTG
GGCCGGGACG CGGCCGAGGA GGTGGCACAG GAGATGGTGG CTCTGGGCTT GGTCTGTGAA
GCCGATTACC AGCCAGTGGC CCGTGCAGTA CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
CGTGAGAAGC TGCGTAAAGC AAGGGAATTG GAGGCACTCC CACCAGAGCC AGGACCTCCA
CCAGCAACTG TGCCCATGGC CCCCGGTCCC CCCAGTGTCT TCCCCCCTGA GCCTGAGGAG
CCAGAGGCAG ACCAGCACCA GCCCTTCCTT TTCCGCCACG CCAGCTACTC ATCTACCACT
TCGGATTGCG AGACTGATGG CTACCTCAGC TCCTCCGGCT TCCTGGATGC CTCAGACCCT
GCCCTTCAGC CCCCTGGGGG GGTGCCATCC AGCCTGGCTG AGTCCCATCT CTGCCTGCCC
TCGGCTTTTG CCCTATCCAT TCCACGTTCT GGCCCTGGAA GTGACTTTTC CCCCGGGGAC
AGCTATGCCT CAGATGCAGC TTCAGGCCTT AGCGATGTGG GAGAAGGGAT GGGACAAATG
AGGAGACCCC CAGGGAGGAA TCTCCGGCGC AGACCCCGAT CCCGGCTGCG GGTCACTAGT
GTCTCAGACC AGAATGACAG AGTGGTTGAG TGCCAGCTAC AGACCCATAA CAGCAAGATG
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCGGAAG AGATTGCAGC TGCCATGGAG
GAGCCAGCAC CATTACCTGC CCTGCCCGTC CCCCTCCCAG ACCCATCCAA TGAAGAGCTC
CAGAGCAGCA CCTCCCTGGA GCACAGGAGC TGGACAGCCT TCTCCACCTC CTCATCTTCT
CCTGGAACTC CTTTGTCTCC TGGAAACCCA TTTTCCCCTG GAACCCCCAT TTCCCCAGGT
CCCATCTTCC CCATCACTTC TCCCCCATGT CATCCCAGCC CCTCCCCATT CTCCCCCATT
TCTTCCCAGG TCTCCTCAAA TCCCTCTCCA CACCCCACCA GCTCTCCACT TCCATTCTCC
TCCAGCACAC CCGAGTTTCC GGTCCCACTC TCTCAGTGTC CCTGGAGTTC TCTCCCCACG
ACTTCTCCAC CTACGTTCTC TCCCACTTGT TCTCAGGTCA CTCTTAGTTC CCCTTTCTTT
CCTCCGTGCC CCTCCACTTC TTCCTTCCCC TCCACCACAG CAGCCCCTCT CCTTTCTCTG
GCTAGTGCCT TCTCACTGGC TGTGATGACT GTGGCCCAGT CCCTGCTGTC CCCCTCACCT
GGGCTCCTTT CCCAGTCTCC TCCAGCCCCT CCTAGTCCCC TCCCTAGCCT GCCCCTTCCC
CCTCCCGTTG CTCCTGGTGG CCAGGAAAGC CCTTCACCCC ACACAGCTGA GGTGGAGAGT
GAGGCCTCAC CACCTCCTGC TCGGCCCCTC CCAGGGGAAG CCAGGCTGGC GCCCATCTCT
GAAGAGGGAA AGCCGCAGCT TGTTGGGCGT TTCCAAGTGA CTTCATCCAA GGAACCGGCT
GAGCCTCTTC CCTTGCAGCC AACATCCCCC ACTCTCTCTG GTTCTCCAAA ACCTTCAACC
CCTCAGCTCA CTTCAGAGAG CTCAGATACA GAGGACAGTG CTGGAGGCGG GCCAGAGACC
AGGGAAGCTC TGGCTGAGAG CGACCGTGCA GCTGAGGGTC TGGGGGCTGG AGTTGAGGAG
GAAGGAGATG ATGGGAAGGA ACCCCAAGTT GGGGGCAGCC CCCAACCCCT GAGCCATCCC
AGCCCAGTGT GGATGAACTA CTCCTACAGC AGCCTGTGTT TGAGCAGCGA GGAGTCAGAA
AGCAGTGGGG AAGATGAGGA GTTCTGGGCT GAGCTGCAGA GTCTTCGGCA GAAGCACTTG
TCAGAGGTGG AAACACTACA GACACTACAG AAAAAAGAAA TTGAAGATTT GTACAGCCGG
CTGGGGAAGC AGCCCCCACC GGGTATTGTG GCCCCAGCTG CTATGCTGTC CAGCCGCCAG
CGCCGCCTCT CCAAGGGCAG CTTCCCCACC TCCCGCCGCA ACAGCCTACA GCGCTCTGAG
CCCCCAGGCC CTGGCATCAT GCGAAGGAAC TCTCTGAGTG GCAGCAGCAC CGGCTCCCAG
GAGCAGCGGG CAAGCAAGGG GGTGACATTC GCCGGGGATG TTGGCAGGAT GGTGAGGGCG
GGCCCAAGGG AGGGAGAGCC CAGGGAATGG TACCTGGCTG CAGCTTCGCC TTCCTCCCAC
CTTGGAGGTT TCTTCATCAC TTTTTCTTTT CCCTCCAGTG AATTCAGAAC AGAAGCCATG
TATCTCCCCC ACACCAGGGC CCACCATGGA GCTTGTGTTC TCAGAATCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016880453.1
CDS999..4769
Translation

Target ORF information:

RefSeq Version XM_017024964.1
Organism Homo sapiens(Human)
Definition Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017024964.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGTTGGCAT CCCCGGCCAC GGAGACCACC GTCCTCATGT CCCAGACTGA GGCCGACCTG 
GCCCTGCGGC CCCCGCCTCC TCTTGGCACC GCGGGGCAGC CCCGCCTCGG GCCCCCTCCT
CGCCGAGCGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC TCGTCTCAGC
CGCCGTAGCT CAGTCGACTT GGGGCTGCTG AGCTCTTGGT CCCTGCCAGC CTCACCCGCT
CCGGACCCCC CCGATCCTCC GGACTCCGCT GGTCCTGGCC CCGCGAGGAG CCCACCGCCT
AGCTCCAAAG AACCCCCCGA GGGCACGTGG ACCGAGGGAG CCCCTGTGAA GGCTGCGGAA
GACTCCGCGC GTCCCGAGCT CCCGGACTCT GCAGTGGGCC CGGGGTCCAG GGAGCCGCTA
AGGGTCCCTG AAGCTGTGGC CCTAGAGCGG CGGCGGGAGC AGGAAGAAAA GGAGGACATG
GAGACCCAGG CTGTGGCAAC GTCCCCCGAT GGCCGATACC TCAAGTTTGA CATCGAGATT
GGACGTGGCT CCTTCAAGAC GGTGTATCGA GGGCTAGACA CCGACACCAC AGTGGAGGTG
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTAGAGCTG AGCGGCAGCG CTTCTCAGAG
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA TGATTCGTGG
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
ACGCTCAAGA CGTACCTGAG GCGGTTCCGG GAGATGAAGC CGCGGGTCCT TCAGCGCTGG
AGCCGCCAAA TCCTGCGGGG ACTTCATTTC CTACACTCCC GGGTTCCTCC CATCCTGCAC
CGGGATCTCA AGTGCGACAA TGTCTTTATC ACGGGACCTA CTGGCTCTGT CAAAATCGGG
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTTGCCA AGAGTGTCAT CGGGACCCCG
GAATTCATGG CCCCCGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
TTCGGCATGT GCATGCTGGA GATGGCCACC TCTGAGTACC CGTACTCCGA GTGCCAGAAT
GCCGCGCAAA TCTACCGCAA GGTCACTTCG GGCAGAAAGC CGAACAGCTT CCACAAGGTG
AAGATACCCG AGGTGAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAACGAGAGG
TTCACCATCC AGGACCTCCT GGCCCACGCC TTCTTCCGCG AGGAGCGCGG TGTGCACGTG
GAACTAGCGG AGGAGGACGA CGGCGAGAAG CCGGGCCTCA AGCTCTGGCT GCGCATGGAG
GACGCGCGGC GCGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCCT GTTCCAGCTG
GGCCGGGACG CGGCCGAGGA GGTGGCACAG GAGATGGTGG CTCTGGGCTT GGTCTGTGAA
GCCGATTACC AGCCAGTGGC CCGTGCAGTA CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
CGTGAGAAGC TGCGTAAAGC AAGGGAATTG GAGGCACTCC CACCAGAGCC AGGACCTCCA
CCAGCAACTG TGCCCATGGC CCCCGGTCCC CCCAGTGTCT TCCCCCCTGA GCCTGAGGAG
CCAGAGGCAG ACCAGCACCA GCCCTTCCTT TTCCGCCACG CCAGCTACTC ATCTACCACT
TCGGATTGCG AGACTGATGG CTACCTCAGC TCCTCCGGCT TCCTGGATGC CTCAGACCCT
GCCCTTCAGC CCCCTGGGGG GGTGCCATCC AGCCTGGCTG AGTCCCATCT CTGCCTGCCC
TCGGCTTTTG CCCTATCCAT TCCACGTTCT GGCCCTGGAA GTGACTTTTC CCCCGGGGAC
AGCTATGCCT CAGATGCAGC TTCAGGCCTT AGCGATGTGG GAGAAGGGAT GGGACAAATG
AGGAGACCCC CAGGGAGGAA TCTCCGGCGC AGACCCCGAT CCCGGCTGCG GGTCACTAGT
GTCTCAGACC AGAATGACAG AGTGGTTGAG TGCCAGCTAC AGACCCATAA CAGCAAGATG
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCGGAAG AGATTGCAGC TGCCATGGAG
GAGCCAGCAC CATTACCTGC CCTGCCCGTC CCCCTCCCAG ACCCATCCAA TGAAGAGCTC
CAGAGCAGCA CCTCCCTGGA GCACAGGAGC TGGACAGCCT TCTCCACCTC CTCATCTTCT
CCTGGAACTC CTTTGTCTCC TGGAAACCCA TTTTCCCCTG GAACCCCCAT TTCCCCAGGT
CCCATCTTCC CCATCACTTC TCCCCCATGT CATCCCAGCC CCTCCCCATT CTCCCCCATT
TCTTCCCAGG TCTCCTCAAA TCCCTCTCCA CACCCCACCA GCTCTCCACT TCCATTCTCC
TCCAGCACAC CCGAGTTTCC GGTCCCACTC TCTCAGTGTC CCTGGAGTTC TCTCCCCACG
ACTTCTCCAC CTACGTTCTC TCCCACTTGT TCTCAGGTCA CTCTTAGTTC CCCTTTCTTT
CCTCCGTGCC CCTCCACTTC TTCCTTCCCC TCCACCACAG CAGCCCCTCT CCTTTCTCTG
GCTAGTGCCT TCTCACTGGC TGTGATGACT GTGGCCCAGT CCCTGCTGTC CCCCTCACCT
GGGCTCCTTT CCCAGTCTCC TCCAGCCCCT CCTAGTCCCC TCCCTAGCCT GCCCCTTCCC
CCTCCCGTTG CTCCTGGTGG CCAGGAAAGC CCTTCACCCC ACACAGCTGA GGTGGAGAGT
GAGGCCTCAC CACCTCCTGC TCGGCCCCTC CCAGGGGAAG CCAGGCTGGC GCCCATCTCT
GAAGAGGGAA AGCCGCAGCT TGTTGGGCGT TTCCAAGTGA CTTCATCCAA GGAACCGGCT
GAGCCTCTTC CCTTGCAGCC AACATCCCCC ACTCTCTCTG GTTCTCCAAA ACCTTCAACC
CCTCAGCTCA CTTCAGAGAG CTCAGATACA GAGGACAGTG CTGGAGGCGG GCCAGAGACC
AGGGAAGCTC TGGCTGAGAG CGACCGTGCA GCTGAGGGTC TGGGGGCTGG AGTTGAGGAG
GAAGGAGATG ATGGGAAGGA ACCCCAAGTT GGGGGCAGCC CCCAACCCCT GAGCCATCCC
AGCCCAGTGT GGATGAACTA CTCCTACAGC AGCCTGTGTT TGAGCAGCGA GGAGTCAGAA
AGCAGTGGGG AAGATGAGGA GTTCTGGGCT GAGCTGCAGA GTCTTCGGCA GAAGCACTTG
TCAGAGGTGG AAACACTACA GACACTACAG AAAAAAGAAA TTGAAGATTT GTACAGCCGG
CTGGGGAAGC AGCCCCCACC GGGTATTGTG GCCCCAGCTG CTATGCTGTC CAGCCGCCAG
CGCCGCCTCT CCAAGGGCAG CTTCCCCACC TCCCGCCGCA ACAGCCTACA GCGCTCTGAG
CCCCCAGGCC CTGGCATCAT GCGAAGGAAC TCTCTGAGTG GCAGCAGCAC CGGCTCCCAG
GAGCAGCGGG CAAGCAAGGG GGTGACATTC GCCGGGGATG TTGGCAGGAT GGTGAGGGCG
GGCCCAAGGG AGGGAGAGCC CAGGGAATGG TACCTGGCTG CAGCTTCGCC TTCCTCCCAC
CTTGGAGGTT TCTTCATCAC TTTTTCTTTT CCCTCCAGTG AATTCAGAAC AGAAGCCATG
TATCTCCCCC ACACCAGGGC CCACCATGGA GCTTGTGTTC TCAGAATCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu87049
Clone ID Related Accession (Same CDS sequence) XM_017024965.1
Accession Version XM_017024965.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3768bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product serine/threonine-protein kinase WNK4 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGTTGGCAT CCCCGGCCAC GGAGACCACC GTCCTCATGT CCCAGACTGA GGCCGACCTG 
GCCCTGCGGC CCCCGCCTCC TCTTGGCACC GCGGGGCAGC CCCGCCTCGG GCCCCCTCCT
CGCCGAGCGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC TCGTCTCAGC
CGCCGTAGCT CAGTCGACTT GGGGCTGCTG AGCTCTTGGT CCCTGCCAGC CTCACCCGCT
CCGGACCCCC CCGATCCTCC GGACTCCGCT GGTCCTGGCC CCGCGAGGAG CCCACCGCCT
AGCTCCAAAG AACCCCCCGA GGGCACGTGG ACCGAGGGAG CCCCTGTGAA GGCTGCGGAA
GACTCCGCGC GTCCCGAGCT CCCGGACTCT GCAGTGGGCC CGGGGTCCAG GGAGCCGCTA
AGGGTCCCTG AAGCTGTGGC CCTAGAGCGG CGGCGGGAGC AGGAAGAAAA GGAGGACATG
GAGACCCAGG CTGTGGCAAC GTCCCCCGAT GGCCGATACC TCAAGTTTGA CATCGAGATT
GGACGTGGCT CCTTCAAGAC GGTGTATCGA GGGCTAGACA CCGACACCAC AGTGGAGGTG
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTAGAGCTG AGCGGCAGCG CTTCTCAGAG
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA TGATTCGTGG
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
ACGCTCAAGA CGTACCTGAG GCGGTTCCGG GAGATGAAGC CGCGGGTCCT TCAGCGCTGG
AGCCGCCAAA TCCTGCGGGG ACTTCATTTC CTACACTCCC GGGTTCCTCC CATCCTGCAC
CGGGATCTCA AGTGCGACAA TGTCTTTATC ACGGGACCTA CTGGCTCTGT CAAAATCGGG
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTTGCCA AGAGTGTCAT CGGGACCCCG
GAATTCATGG CCCCCGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
TTCGGCATGT GCATGCTGGA GATGGCCACC TCTGAGTACC CGTACTCCGA GTGCCAGAAT
GCCGCGCAAA TCTACCGCAA GGTCACTTCG GGCAGAAAGC CGAACAGCTT CCACAAGGTG
AAGATACCCG AGGTGAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAACGAGAGG
TTCACCATCC AGGACCTCCT GGCCCACGCC TTCTTCCGCG AGGAGCGCGG TGTGCACGTG
GAACTAGCGG AGGAGGACGA CGGCGAGAAG CCGGGCCTCA AGCTCTGGCT GCGCATGGAG
GACGCGCGGC GCGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCCT GTTCCAGCTG
GGCCGGGACG CGGCCGAGGA GGTGGCACAG GAGATGGTGG CTCTGGGCTT GGTCTGTGAA
GCCGATTACC AGCCAGTGGC CCGTGCAGTA CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
CGTGAGAAGC TGCGTAAAGC AAGGGAATTG GAGGCACTCC CACCAGAGCC AGGACCTCCA
CCAGCAACTG TGCCCATGGC CCCCGGTCCC CCCAGTGTCT TCCCCCCTGA GCCTGAGGAG
CCAGAGGCAG ACCAGCACCA GCCCTTCCTT TTCCGCCACG CCAGCTACTC ATCTACCACT
TCGGATTGCG AGACTGATGG CTACCTCAGC TCCTCCGGCT TCCTGGATGC CTCAGACCCT
GCCCTTCAGC CCCCTGGGGG GGTGCCATCC AGCCTGGCTG AGTCCCATCT CTGCCTGCCC
TCGGCTTTTG CCCTATCCAT TCCACGTTCT GGCCCTGGAA GTGACTTTTC CCCCGGGGAC
AGCTATGCCT CAGATGCAGC TTCAGGCCTT AGCGATGTGG GAGAAGGGAT GGGACAAATG
AGGAGACCCC CAGGGAGGAA TCTCCGGCGC AGACCCCGAT CCCGGCTGCG GGTCACTAGT
GTCTCAGACC AGAATGACAG AGTGGTTGAG TGCCAGCTAC AGACCCATAA CAGCAAGATG
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCGGAAG AGATTGCAGC TGCCATGGAG
GAGCCAGCAC CATTACCTGC CCTGCCCGTC CCCCTCCCAG ACCCATCCAA TGAGCTCCAG
AGCAGCACCT CCCTGGAGCA CAGGAGCTGG ACAGCCTTCT CCACCTCCTC ATCTTCTCCT
GGAACTCCTT TGTCTCCTGG AAACCCATTT TCCCCTGGAA CCCCCATTTC CCCAGGTCCC
ATCTTCCCCA TCACTTCTCC CCCATGTCAT CCCAGCCCCT CCCCATTCTC CCCCATTTCT
TCCCAGGTCT CCTCAAATCC CTCTCCACAC CCCACCAGCT CTCCACTTCC ATTCTCCTCC
AGCACACCCG AGTTTCCGGT CCCACTCTCT CAGTGTCCCT GGAGTTCTCT CCCCACGACT
TCTCCACCTA CGTTCTCTCC CACTTGTTCT CAGGTCACTC TTAGTTCCCC TTTCTTTCCT
CCGTGCCCCT CCACTTCTTC CTTCCCCTCC ACCACAGCAG CCCCTCTCCT TTCTCTGGCT
AGTGCCTTCT CACTGGCTGT GATGACTGTG GCCCAGTCCC TGCTGTCCCC CTCACCTGGG
CTCCTTTCCC AGTCTCCTCC AGCCCCTCCT AGTCCCCTCC CTAGCCTGCC CCTTCCCCCT
CCCGTTGCTC CTGGTGGCCA GGAAAGCCCT TCACCCCACA CAGCTGAGGT GGAGAGTGAG
GCCTCACCAC CTCCTGCTCG GCCCCTCCCA GGGGAAGCCA GGCTGGCGCC CATCTCTGAA
GAGGGAAAGC CGCAGCTTGT TGGGCGTTTC CAAGTGACTT CATCCAAGGA ACCGGCTGAG
CCTCTTCCCT TGCAGCCAAC ATCCCCCACT CTCTCTGGTT CTCCAAAACC TTCAACCCCT
CAGCTCACTT CAGAGAGCTC AGATACAGAG GACAGTGCTG GAGGCGGGCC AGAGACCAGG
GAAGCTCTGG CTGAGAGCGA CCGTGCAGCT GAGGGTCTGG GGGCTGGAGT TGAGGAGGAA
GGAGATGATG GGAAGGAACC CCAAGTTGGG GGCAGCCCCC AACCCCTGAG CCATCCCAGC
CCAGTGTGGA TGAACTACTC CTACAGCAGC CTGTGTTTGA GCAGCGAGGA GTCAGAAAGC
AGTGGGGAAG ATGAGGAGTT CTGGGCTGAG CTGCAGAGTC TTCGGCAGAA GCACTTGTCA
GAGGTGGAAA CACTACAGAC ACTACAGAAA AAAGAAATTG AAGATTTGTA CAGCCGGCTG
GGGAAGCAGC CCCCACCGGG TATTGTGGCC CCAGCTGCTA TGCTGTCCAG CCGCCAGCGC
CGCCTCTCCA AGGGCAGCTT CCCCACCTCC CGCCGCAACA GCCTACAGCG CTCTGAGCCC
CCAGGCCCTG GCATCATGCG AAGGAACTCT CTGAGTGGCA GCAGCACCGG CTCCCAGGAG
CAGCGGGCAA GCAAGGGGGT GACATTCGCC GGGGATGTTG GCAGGATGGT GAGGGCGGGC
CCAAGGGAGG GAGAGCCCAG GGAATGGTAC CTGGCTGCAG CTTCGCCTTC CTCCCACCTT
GGAGGTTTCT TCATCACTTT TTCTTTTCCC TCCAGTGAAT TCAGAACAGA AGCCATGTAT
CTCCCCCACA CCAGGGCCCA CCATGGAGCT TGTGTTCTCA GAATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016880454.1
CDS999..4766
Translation

Target ORF information:

RefSeq Version XM_017024965.1
Organism Homo sapiens(Human)
Definition Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017024965.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGTTGGCAT CCCCGGCCAC GGAGACCACC GTCCTCATGT CCCAGACTGA GGCCGACCTG 
GCCCTGCGGC CCCCGCCTCC TCTTGGCACC GCGGGGCAGC CCCGCCTCGG GCCCCCTCCT
CGCCGAGCGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC TCGTCTCAGC
CGCCGTAGCT CAGTCGACTT GGGGCTGCTG AGCTCTTGGT CCCTGCCAGC CTCACCCGCT
CCGGACCCCC CCGATCCTCC GGACTCCGCT GGTCCTGGCC CCGCGAGGAG CCCACCGCCT
AGCTCCAAAG AACCCCCCGA GGGCACGTGG ACCGAGGGAG CCCCTGTGAA GGCTGCGGAA
GACTCCGCGC GTCCCGAGCT CCCGGACTCT GCAGTGGGCC CGGGGTCCAG GGAGCCGCTA
AGGGTCCCTG AAGCTGTGGC CCTAGAGCGG CGGCGGGAGC AGGAAGAAAA GGAGGACATG
GAGACCCAGG CTGTGGCAAC GTCCCCCGAT GGCCGATACC TCAAGTTTGA CATCGAGATT
GGACGTGGCT CCTTCAAGAC GGTGTATCGA GGGCTAGACA CCGACACCAC AGTGGAGGTG
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTAGAGCTG AGCGGCAGCG CTTCTCAGAG
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA TGATTCGTGG
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
ACGCTCAAGA CGTACCTGAG GCGGTTCCGG GAGATGAAGC CGCGGGTCCT TCAGCGCTGG
AGCCGCCAAA TCCTGCGGGG ACTTCATTTC CTACACTCCC GGGTTCCTCC CATCCTGCAC
CGGGATCTCA AGTGCGACAA TGTCTTTATC ACGGGACCTA CTGGCTCTGT CAAAATCGGG
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTTGCCA AGAGTGTCAT CGGGACCCCG
GAATTCATGG CCCCCGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
TTCGGCATGT GCATGCTGGA GATGGCCACC TCTGAGTACC CGTACTCCGA GTGCCAGAAT
GCCGCGCAAA TCTACCGCAA GGTCACTTCG GGCAGAAAGC CGAACAGCTT CCACAAGGTG
AAGATACCCG AGGTGAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAACGAGAGG
TTCACCATCC AGGACCTCCT GGCCCACGCC TTCTTCCGCG AGGAGCGCGG TGTGCACGTG
GAACTAGCGG AGGAGGACGA CGGCGAGAAG CCGGGCCTCA AGCTCTGGCT GCGCATGGAG
GACGCGCGGC GCGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCCT GTTCCAGCTG
GGCCGGGACG CGGCCGAGGA GGTGGCACAG GAGATGGTGG CTCTGGGCTT GGTCTGTGAA
GCCGATTACC AGCCAGTGGC CCGTGCAGTA CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
CGTGAGAAGC TGCGTAAAGC AAGGGAATTG GAGGCACTCC CACCAGAGCC AGGACCTCCA
CCAGCAACTG TGCCCATGGC CCCCGGTCCC CCCAGTGTCT TCCCCCCTGA GCCTGAGGAG
CCAGAGGCAG ACCAGCACCA GCCCTTCCTT TTCCGCCACG CCAGCTACTC ATCTACCACT
TCGGATTGCG AGACTGATGG CTACCTCAGC TCCTCCGGCT TCCTGGATGC CTCAGACCCT
GCCCTTCAGC CCCCTGGGGG GGTGCCATCC AGCCTGGCTG AGTCCCATCT CTGCCTGCCC
TCGGCTTTTG CCCTATCCAT TCCACGTTCT GGCCCTGGAA GTGACTTTTC CCCCGGGGAC
AGCTATGCCT CAGATGCAGC TTCAGGCCTT AGCGATGTGG GAGAAGGGAT GGGACAAATG
AGGAGACCCC CAGGGAGGAA TCTCCGGCGC AGACCCCGAT CCCGGCTGCG GGTCACTAGT
GTCTCAGACC AGAATGACAG AGTGGTTGAG TGCCAGCTAC AGACCCATAA CAGCAAGATG
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCGGAAG AGATTGCAGC TGCCATGGAG
GAGCCAGCAC CATTACCTGC CCTGCCCGTC CCCCTCCCAG ACCCATCCAA TGAGCTCCAG
AGCAGCACCT CCCTGGAGCA CAGGAGCTGG ACAGCCTTCT CCACCTCCTC ATCTTCTCCT
GGAACTCCTT TGTCTCCTGG AAACCCATTT TCCCCTGGAA CCCCCATTTC CCCAGGTCCC
ATCTTCCCCA TCACTTCTCC CCCATGTCAT CCCAGCCCCT CCCCATTCTC CCCCATTTCT
TCCCAGGTCT CCTCAAATCC CTCTCCACAC CCCACCAGCT CTCCACTTCC ATTCTCCTCC
AGCACACCCG AGTTTCCGGT CCCACTCTCT CAGTGTCCCT GGAGTTCTCT CCCCACGACT
TCTCCACCTA CGTTCTCTCC CACTTGTTCT CAGGTCACTC TTAGTTCCCC TTTCTTTCCT
CCGTGCCCCT CCACTTCTTC CTTCCCCTCC ACCACAGCAG CCCCTCTCCT TTCTCTGGCT
AGTGCCTTCT CACTGGCTGT GATGACTGTG GCCCAGTCCC TGCTGTCCCC CTCACCTGGG
CTCCTTTCCC AGTCTCCTCC AGCCCCTCCT AGTCCCCTCC CTAGCCTGCC CCTTCCCCCT
CCCGTTGCTC CTGGTGGCCA GGAAAGCCCT TCACCCCACA CAGCTGAGGT GGAGAGTGAG
GCCTCACCAC CTCCTGCTCG GCCCCTCCCA GGGGAAGCCA GGCTGGCGCC CATCTCTGAA
GAGGGAAAGC CGCAGCTTGT TGGGCGTTTC CAAGTGACTT CATCCAAGGA ACCGGCTGAG
CCTCTTCCCT TGCAGCCAAC ATCCCCCACT CTCTCTGGTT CTCCAAAACC TTCAACCCCT
CAGCTCACTT CAGAGAGCTC AGATACAGAG GACAGTGCTG GAGGCGGGCC AGAGACCAGG
GAAGCTCTGG CTGAGAGCGA CCGTGCAGCT GAGGGTCTGG GGGCTGGAGT TGAGGAGGAA
GGAGATGATG GGAAGGAACC CCAAGTTGGG GGCAGCCCCC AACCCCTGAG CCATCCCAGC
CCAGTGTGGA TGAACTACTC CTACAGCAGC CTGTGTTTGA GCAGCGAGGA GTCAGAAAGC
AGTGGGGAAG ATGAGGAGTT CTGGGCTGAG CTGCAGAGTC TTCGGCAGAA GCACTTGTCA
GAGGTGGAAA CACTACAGAC ACTACAGAAA AAAGAAATTG AAGATTTGTA CAGCCGGCTG
GGGAAGCAGC CCCCACCGGG TATTGTGGCC CCAGCTGCTA TGCTGTCCAG CCGCCAGCGC
CGCCTCTCCA AGGGCAGCTT CCCCACCTCC CGCCGCAACA GCCTACAGCG CTCTGAGCCC
CCAGGCCCTG GCATCATGCG AAGGAACTCT CTGAGTGGCA GCAGCACCGG CTCCCAGGAG
CAGCGGGCAA GCAAGGGGGT GACATTCGCC GGGGATGTTG GCAGGATGGT GAGGGCGGGC
CCAAGGGAGG GAGAGCCCAG GGAATGGTAC CTGGCTGCAG CTTCGCCTTC CTCCCACCTT
GGAGGTTTCT TCATCACTTT TTCTTTTCCC TCCAGTGAAT TCAGAACAGA AGCCATGTAT
CTCCCCCACA CCAGGGCCCA CCATGGAGCT TGTGTTCTCA GAATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17844
Clone ID Related Accession (Same CDS sequence) NM_032387.4
Accession Version NM_032387.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3732bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-03-19
Organism Homo sapiens(Human)
Product serine/threonine-protein kinase WNK4 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CA388932.1, BC136664.1 and BM683764.1. This sequence is a reference standard in the RefSeqGene project. On Oct 1, 2009 this sequence version replaced NM_032387.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC136664.1, AJ316534.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGTTGGCAT CCCCGGCCAC GGAGACCACC GTCCTCATGT CCCAGACTGA GGCCGACCTG 
GCCCTGCGGC CCCCGCCTCC TCTTGGCACC GCGGGGCAGC CCCGCCTCGG GCCCCCTCCT
CGCCGAGCGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC TCGTCTCAGC
CGCCGTAGCT CAGTCGACTT GGGGCTGCTG AGCTCTTGGT CCCTGCCAGC CTCACCCGCT
CCGGACCCCC CCGATCCTCC GGACTCCGCT GGTCCTGGCC CCGCGAGGAG CCCACCGCCT
AGCTCCAAAG AACCCCCCGA GGGCACGTGG ACCGAGGGAG CCCCTGTGAA GGCTGCGGAA
GACTCCGCGC GTCCCGAGCT CCCGGACTCT GCAGTGGGCC CGGGGTCCAG GGAGCCGCTA
AGGGTCCCTG AAGCTGTGGC CCTAGAGCGG CGGCGGGAGC AGGAAGAAAA GGAGGACATG
GAGACCCAGG CTGTGGCAAC GTCCCCCGAT GGCCGATACC TCAAGTTTGA CATCGAGATT
GGACGTGGCT CCTTCAAGAC GGTGTATCGA GGGCTAGACA CCGACACCAC AGTGGAGGTG
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTAGAGCTG AGCGGCAGCG CTTCTCAGAG
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA TGATTCGTGG
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
ACGCTCAAGA CGTACCTGAG GCGGTTCCGG GAGATGAAGC CGCGGGTCCT TCAGCGCTGG
AGCCGCCAAA TCCTGCGGGG ACTTCATTTC CTACACTCCC GGGTTCCTCC CATCCTGCAC
CGGGATCTCA AGTGCGACAA TGTCTTTATC ACGGGACCTA CTGGCTCTGT CAAAATCGGG
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTTGCCA AGAGTGTCAT CGGGACCCCG
GAATTCATGG CCCCCGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
TTCGGCATGT GCATGCTGGA GATGGCCACC TCTGAGTACC CGTACTCCGA GTGCCAGAAT
GCCGCGCAAA TCTACCGCAA GGTCACTTCG GGCAGAAAGC CGAACAGCTT CCACAAGGTG
AAGATACCCG AGGTGAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAACGAGAGG
TTCACCATCC AGGACCTCCT GGCCCACGCC TTCTTCCGCG AGGAGCGCGG TGTGCACGTG
GAACTAGCGG AGGAGGACGA CGGCGAGAAG CCGGGCCTCA AGCTCTGGCT GCGCATGGAG
GACGCGCGGC GCGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCCT GTTCCAGCTG
GGCCGGGACG CGGCCGAGGA GGTGGCACAG GAGATGGTGG CTCTGGGCTT GGTCTGTGAA
GCCGATTACC AGCCAGTGGC CCGTGCAGTA CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
CGTGAGAAGC TGCGTAAAGC AAGGGAATTG GAGGCACTCC CACCAGAGCC AGGACCTCCA
CCAGCAACTG TGCCCATGGC CCCCGGTCCC CCCAGTGTCT TCCCCCCTGA GCCTGAGGAG
CCAGAGGCAG ACCAGCACCA GCCCTTCCTT TTCCGCCACG CCAGCTACTC ATCTACCACT
TCGGATTGCG AGACTGATGG CTACCTCAGC TCCTCCGGCT TCCTGGATGC CTCAGACCCT
GCCCTTCAGC CCCCTGGGGG GGTGCCATCC AGCCTGGCTG AGTCCCATCT CTGCCTGCCC
TCGGCTTTTG CCCTATCCAT TCCACGTTCT GGCCCTGGAA GTGACTTTTC CCCCGGGGAC
AGCTATGCCT CAGATGCAGC TTCAGGCCTT AGCGATGTGG GAGAAGGGAT GGGACAAATG
AGGAGACCCC CAGGGAGGAA TCTCCGGCGC AGACCCCGAT CCCGGCTGCG GGTCACTAGT
GTCTCAGACC AGAATGACAG AGTGGTTGAG TGCCAGCTAC AGACCCATAA CAGCAAGATG
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCGGAAG AGATTGCAGC TGCCATGGTA
TATAACGAGT TCATTCTGCC TTCGGAGCGA GATGGATTTC TCAGACGGAT TCGGGAGATT
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GACACTGGCC CCATGGAGGC TGCTGAAGAC
ACCCTAAGCC CCCAGGAGGA GCCAGCACCA TTACCTGCCC TGCCCGTCCC CCTCCCAGAC
CCATCCAATG AAGAGCTCCA GAGCAGCACC TCCCTGGAGC ACAGGAGCTG GACAGCCTTC
TCCACCTCCT CATCTTCTCC TGGAACTCCT TTGTCTCCTG GAAACCCATT TTCCCCTGGA
ACCCCCATTT CCCCAGGTCC CATCTTCCCC ATCACTTCTC CCCCATGTCA TCCCAGCCCC
TCCCCATTCT CCCCCATTTC TTCCCAGGTC TCCTCAAATC CCTCTCCACA CCCCACCAGC
TCTCCACTTC CATTCTCCTC CAGCACACCC GAGTTTCCGG TCCCACTCTC TCAGTGTCCC
TGGAGTTCTC TCCCCACGAC TTCTCCACCT ACGTTCTCTC CCACTTGTTC TCAGGTCACT
CTTAGTTCCC CTTTCTTTCC TCCGTGCCCC TCCACTTCTT CCTTCCCCTC CACCACAGCA
GCCCCTCTCC TTTCTCTGGC TAGTGCCTTC TCACTGGCTG TGATGACTGT GGCCCAGTCC
CTGCTGTCCC CCTCACCTGG GCTCCTTTCC CAGTCTCCTC CAGCCCCTCC TAGTCCCCTC
CCTAGCCTGC CCCTTCCCCC TCCCGTTGCT CCTGGTGGCC AGGAAAGCCC TTCACCCCAC
ACAGCTGAGG TGGAGAGTGA GGCCTCACCA CCTCCTGCTC GGCCCCTCCC AGGGGAAGCC
AGGCTGGCGC CCATCTCTGA AGAGGGAAAG CCGCAGCTTG TTGGGCGTTT CCAAGTGACT
TCATCCAAGG AACCGGCTGA GCCTCTTCCC TTGCAGCCAA CATCCCCCAC TCTCTCTGGT
TCTCCAAAAC CTTCAACCCC TCAGCTCACT TCAGAGAGCT CAGATACAGA GGACAGTGCT
GGAGGCGGGC CAGAGACCAG GGAAGCTCTG GCTGAGAGCG ACCGTGCAGC TGAGGGTCTG
GGGGCTGGAG TTGAGGAGGA AGGAGATGAT GGGAAGGAAC CCCAAGTTGG GGGCAGCCCC
CAACCCCTGA GCCATCCCAG CCCAGTGTGG ATGAACTACT CCTACAGCAG CCTGTGTTTG
AGCAGCGAGG AGTCAGAAAG CAGTGGGGAA GATGAGGAGT TCTGGGCTGA GCTGCAGAGT
CTTCGGCAGA AGCACTTGTC AGAGGTGGAA ACACTACAGA CACTACAGAA AAAAGAAATT
GAAGATTTGT ACAGCCGGCT GGGGAAGCAG CCCCCACCGG GTATTGTGGC CCCAGCTGCT
ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC AAGGGCAGCT TCCCCACCTC CCGCCGCAAC
AGCCTACAGC GCTCTGAGCC CCCAGGCCCT GGCATCATGC GAAGGAACTC TCTGAGTGGC
AGCAGCACCG GCTCCCAGGA GCAGCGGGCA AGCAAGGGGG TGACATTCGC CGGGGATGTT
GGCAGGATGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_115763.2
CDS69..3800
Misc Feature(1)582..1364
Misc Feature(2)603..1364
Misc Feature(3)order(606..620,624..626,630..632,669..671,675..677,768..770,828..839,849..851,855..857,972..974,978..980,984..989,993..995,1029..1031,1038..1040,1080..1091)
Misc Feature(4)order(606..620,624..626,630..632,669..671,675..677,768..770,828..839,849..851,972..974,978..980,984..989,993..995,1029..1031)
Misc Feature(5)order(618..620,849..851,855..857,972..974,978..980,984..986,1038..1040,1080..1091)
Misc Feature(6)1026..1091
Misc Feature(7)1059..1061
Misc Feature(8)1071..1073
Misc Feature(9)1425..1535
Misc Feature(10)3171..3173
Misc Feature(11)3717..3719
Exon (1)1..686
Exon (2)687..859
Exon (3)860..1080
Exon (4)1081..1238
Exon (5)1239..1327
Exon (6)1328..1544
Exon (7)1545..1809
Exon (8)1810..1931
Exon (9)1932..1990
Exon (10)1991..2108
Exon (11)2109..2225
Exon (12)2226..2363
Exon (13)2364..2418
Exon (14)2419..3029
Exon (15)3030..3090
Exon (16)3091..3499
Exon (17)3500..3699
Exon (18)3700..3797
Exon (19)3798..4178
Translation

Target ORF information:

RefSeq Version NM_032387.4
Organism Homo sapiens(Human)
Definition Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_032387.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGTTGGCAT CCCCGGCCAC GGAGACCACC GTCCTCATGT CCCAGACTGA GGCCGACCTG 
GCCCTGCGGC CCCCGCCTCC TCTTGGCACC GCGGGGCAGC CCCGCCTCGG GCCCCCTCCT
CGCCGAGCGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC TCGTCTCAGC
CGCCGTAGCT CAGTCGACTT GGGGCTGCTG AGCTCTTGGT CCCTGCCAGC CTCACCCGCT
CCGGACCCCC CCGATCCTCC GGACTCCGCT GGTCCTGGCC CCGCGAGGAG CCCACCGCCT
AGCTCCAAAG AACCCCCCGA GGGCACGTGG ACCGAGGGAG CCCCTGTGAA GGCTGCGGAA
GACTCCGCGC GTCCCGAGCT CCCGGACTCT GCAGTGGGCC CGGGGTCCAG GGAGCCGCTA
AGGGTCCCTG AAGCTGTGGC CCTAGAGCGG CGGCGGGAGC AGGAAGAAAA GGAGGACATG
GAGACCCAGG CTGTGGCAAC GTCCCCCGAT GGCCGATACC TCAAGTTTGA CATCGAGATT
GGACGTGGCT CCTTCAAGAC GGTGTATCGA GGGCTAGACA CCGACACCAC AGTGGAGGTG
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTAGAGCTG AGCGGCAGCG CTTCTCAGAG
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA TGATTCGTGG
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
ACGCTCAAGA CGTACCTGAG GCGGTTCCGG GAGATGAAGC CGCGGGTCCT TCAGCGCTGG
AGCCGCCAAA TCCTGCGGGG ACTTCATTTC CTACACTCCC GGGTTCCTCC CATCCTGCAC
CGGGATCTCA AGTGCGACAA TGTCTTTATC ACGGGACCTA CTGGCTCTGT CAAAATCGGG
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTTGCCA AGAGTGTCAT CGGGACCCCG
GAATTCATGG CCCCCGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
TTCGGCATGT GCATGCTGGA GATGGCCACC TCTGAGTACC CGTACTCCGA GTGCCAGAAT
GCCGCGCAAA TCTACCGCAA GGTCACTTCG GGCAGAAAGC CGAACAGCTT CCACAAGGTG
AAGATACCCG AGGTGAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAACGAGAGG
TTCACCATCC AGGACCTCCT GGCCCACGCC TTCTTCCGCG AGGAGCGCGG TGTGCACGTG
GAACTAGCGG AGGAGGACGA CGGCGAGAAG CCGGGCCTCA AGCTCTGGCT GCGCATGGAG
GACGCGCGGC GCGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCCT GTTCCAGCTG
GGCCGGGACG CGGCCGAGGA GGTGGCACAG GAGATGGTGG CTCTGGGCTT GGTCTGTGAA
GCCGATTACC AGCCAGTGGC CCGTGCAGTA CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
CGTGAGAAGC TGCGTAAAGC AAGGGAATTG GAGGCACTCC CACCAGAGCC AGGACCTCCA
CCAGCAACTG TGCCCATGGC CCCCGGTCCC CCCAGTGTCT TCCCCCCTGA GCCTGAGGAG
CCAGAGGCAG ACCAGCACCA GCCCTTCCTT TTCCGCCACG CCAGCTACTC ATCTACCACT
TCGGATTGCG AGACTGATGG CTACCTCAGC TCCTCCGGCT TCCTGGATGC CTCAGACCCT
GCCCTTCAGC CCCCTGGGGG GGTGCCATCC AGCCTGGCTG AGTCCCATCT CTGCCTGCCC
TCGGCTTTTG CCCTATCCAT TCCACGTTCT GGCCCTGGAA GTGACTTTTC CCCCGGGGAC
AGCTATGCCT CAGATGCAGC TTCAGGCCTT AGCGATGTGG GAGAAGGGAT GGGACAAATG
AGGAGACCCC CAGGGAGGAA TCTCCGGCGC AGACCCCGAT CCCGGCTGCG GGTCACTAGT
GTCTCAGACC AGAATGACAG AGTGGTTGAG TGCCAGCTAC AGACCCATAA CAGCAAGATG
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCGGAAG AGATTGCAGC TGCCATGGTA
TATAACGAGT TCATTCTGCC TTCGGAGCGA GATGGATTTC TCAGACGGAT TCGGGAGATT
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GACACTGGCC CCATGGAGGC TGCTGAAGAC
ACCCTAAGCC CCCAGGAGGA GCCAGCACCA TTACCTGCCC TGCCCGTCCC CCTCCCAGAC
CCATCCAATG AAGAGCTCCA GAGCAGCACC TCCCTGGAGC ACAGGAGCTG GACAGCCTTC
TCCACCTCCT CATCTTCTCC TGGAACTCCT TTGTCTCCTG GAAACCCATT TTCCCCTGGA
ACCCCCATTT CCCCAGGTCC CATCTTCCCC ATCACTTCTC CCCCATGTCA TCCCAGCCCC
TCCCCATTCT CCCCCATTTC TTCCCAGGTC TCCTCAAATC CCTCTCCACA CCCCACCAGC
TCTCCACTTC CATTCTCCTC CAGCACACCC GAGTTTCCGG TCCCACTCTC TCAGTGTCCC
TGGAGTTCTC TCCCCACGAC TTCTCCACCT ACGTTCTCTC CCACTTGTTC TCAGGTCACT
CTTAGTTCCC CTTTCTTTCC TCCGTGCCCC TCCACTTCTT CCTTCCCCTC CACCACAGCA
GCCCCTCTCC TTTCTCTGGC TAGTGCCTTC TCACTGGCTG TGATGACTGT GGCCCAGTCC
CTGCTGTCCC CCTCACCTGG GCTCCTTTCC CAGTCTCCTC CAGCCCCTCC TAGTCCCCTC
CCTAGCCTGC CCCTTCCCCC TCCCGTTGCT CCTGGTGGCC AGGAAAGCCC TTCACCCCAC
ACAGCTGAGG TGGAGAGTGA GGCCTCACCA CCTCCTGCTC GGCCCCTCCC AGGGGAAGCC
AGGCTGGCGC CCATCTCTGA AGAGGGAAAG CCGCAGCTTG TTGGGCGTTT CCAAGTGACT
TCATCCAAGG AACCGGCTGA GCCTCTTCCC TTGCAGCCAA CATCCCCCAC TCTCTCTGGT
TCTCCAAAAC CTTCAACCCC TCAGCTCACT TCAGAGAGCT CAGATACAGA GGACAGTGCT
GGAGGCGGGC CAGAGACCAG GGAAGCTCTG GCTGAGAGCG ACCGTGCAGC TGAGGGTCTG
GGGGCTGGAG TTGAGGAGGA AGGAGATGAT GGGAAGGAAC CCCAAGTTGG GGGCAGCCCC
CAACCCCTGA GCCATCCCAG CCCAGTGTGG ATGAACTACT CCTACAGCAG CCTGTGTTTG
AGCAGCGAGG AGTCAGAAAG CAGTGGGGAA GATGAGGAGT TCTGGGCTGA GCTGCAGAGT
CTTCGGCAGA AGCACTTGTC AGAGGTGGAA ACACTACAGA CACTACAGAA AAAAGAAATT
GAAGATTTGT ACAGCCGGCT GGGGAAGCAG CCCCCACCGG GTATTGTGGC CCCAGCTGCT
ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC AAGGGCAGCT TCCCCACCTC CCGCCGCAAC
AGCCTACAGC GCTCTGAGCC CCCAGGCCCT GGCATCATGC GAAGGAACTC TCTGAGTGGC
AGCAGCACCG GCTCCCAGGA GCAGCGGGCA AGCAAGGGGG TGACATTCGC CGGGGATGTT
GGCAGGATGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71644
Clone ID Related Accession (Same CDS sequence) XM_011525135.2
Accession Version XM_011525135.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3690bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product serine/threonine-protein kinase WNK4 isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011525135.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGTTGGCAT CCCCGGCCAC GGAGACCACC GTCCTCATGT CCCAGACTGA GGCCGACCTG 
GCCCTGCGGC CCCCGCCTCC TCTTGGCACC GCGGGGCAGC CCCGCCTCGG GCCCCCTCCT
CGCCGAGCGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC TCGTCTCAGC
CGCCGTAGCT CAGTCGACTT GGGGCTGCTG AGCTCTTGGT CCCTGCCAGC CTCACCCGCT
CCGGACCCCC CCGATCCTCC GGACTCCGCT GGTCCTGGCC CCGCGAGGAG CCCACCGCCT
AGCTCCAAAG AACCCCCCGA GGGCACGTGG ACCGAGGGAG CCCCTGTGAA GGCTGCGGAA
GACTCCGCGC GTCCCGAGCT CCCGGACTCT GCAGTGGGCC CGGGGTCCAG GGAGCCGCTA
AGGGTCCCTG AAGCTGTGGC CCTAGAGCGG CGGCGGGAGC AGGAAGAAAA GGAGGACATG
GAGACCCAGG CTGTGGCAAC GTCCCCCGAT GGCCGATACC TCAAGTTTGA CATCGAGATT
GGACGTGGCT CCTTCAAGAC GGTGTATCGA GGGCTAGACA CCGACACCAC AGTGGAGGTG
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTAGAGCTG AGCGGCAGCG CTTCTCAGAG
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA TGATTCGTGG
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
ACGCTCAAGA CGTACCTGAG GCGGTTCCGG GAGATGAAGC CGCGGGTCCT TCAGCGCTGG
AGCCGCCAAA TCCTGCGGGG ACTTCATTTC CTACACTCCC GGGTTCCTCC CATCCTGCAC
CGGGATCTCA AGTGCGACAA TGTCTTTATC ACGGGACCTA CTGGCTCTGT CAAAATCGGG
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTTGCCA AGAGTGTCAT CGGGACCCCG
GAATTCATGG CCCCCGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
TTCGGCATGT GCATGCTGGA GATGGCCACC TCTGAGTACC CGTACTCCGA GTGCCAGAAT
GCCGCGCAAA TCTACCGCAA GGTCACTTCG GGCAGAAAGC CGAACAGCTT CCACAAGGTG
AAGATACCCG AGGTGAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAACGAGAGG
TTCACCATCC AGGACCTCCT GGCCCACGCC TTCTTCCGCG AGGAGCGCGG TGTGCACGTG
GAACTAGCGG AGGAGGACGA CGGCGAGAAG CCGGGCCTCA AGCTCTGGCT GCGCATGGAG
GACGCGCGGC GCGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCCT GTTCCAGCTG
GGCCGGGACG CGGCCGAGGA GGTGGCACAG GAGATGGTGG CTCTGGGCTT GGTCTGTGAA
GCCGATTACC AGCCAGTGGC CCGTGCAGTA CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
CGTGAGAAGC TGCGTAAAGC AAGGGAATTG GAGGCACTCC CACCAGAGCC AGGACCTCCA
CCAGCAACTG TGCCCATGGC CCCCGGTCCC CCCAGTGTCT TCCCCCCTGA GCCTGAGGAG
CCAGAGGCAG ACCAGCACCA GCCCTTCCTT TTCCGCCACG CCAGCTACTC ATCTACCACT
TCGGATTGCG AGACTGATGG CTACCTCAGC TCCTCCGGCT TCCTGGATGC CTCAGACCCT
GCCCTTCAGC CCCCTGGGGG GGTGCCATCC AGCCTGGCTG AGTCCCATCT CTGCCTGCCC
TCGGCTTTTG CCCTATCCAT TCCACGTTCT GGCCCTGGAA GTGACTTTTC CCCCGGGGAC
AGCTATGCCT CAGATGCAGC TTCAGGCCTT AGCGATGTGG GAGAAGGGAT GGGACAAATG
AGGAGACCCC CAGGGAGGAA TCTCCGGCGC AGACCCCGAT CCCGGCTGCG GGTCACTAGT
GTCTCAGACC AGAATGACAG AGTGGTTGAG TGCCAGCTAC AGACCCATAA CAGCAAGATG
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCGGAAG AGATTGCAGC TGCCATGGTA
TATAACGAGT TCATTCTGCC TTCGGAGCGA GATGGATTTC TCAGACGGAT TCGGGAGATT
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GACACTGGCC CCATGGAGGC TGCTGAAGAC
ACCCTAAGCC CCCAGGAGGA GCCAGCACCA TTACCTGCCC TGCCCGTCCC CCTCCCAGAC
CCATCCAATG AAGAGCTCCA GAGCAGCACC TCCCTGGAGC ACAGGAGCTG GACAGCCTTC
TCCACCTCCT CATCTTCTCC TGGAACTCCT TTGTCTCCTG GAAACCCATT TTCCCCTGGA
ACCCCCATTT CCCCAGGTCC CATCTTCCCC ATCACTTCTC CCCCATGTCA TCCCAGCCCC
TCCCCATTCT CCCCCATTTC TTCCCAGGTC TCCTCAAATC CCTCTCCACA CCCCACCAGC
TCTCCACTTC CATTCTCCTC CAGCACACCC GAGTTTCCGG TCCCACTCTC TCAGTGTCCC
TGGAGTTCTC TCCCCACGAC TTCTCCACCT ACGTTCTCTC CCACTTGTTC TCAGGTCACT
CTTAGTTCCC CTTTCTTTCC TCCGTGCCCC TCCACTTCTT CCTTCCCCTC CACCACAGCA
GCCCCTCTCC TTTCTCTGGC TAGTGCCTTC TCACTGGCTG TGATGACTGT GGCCCAGTCC
CTGCTGTCCC CCTCACCTGG GCTCCTTTCC CAGTCTCCTC CAGCCCCTCC TAGTCCCCTC
CCTAGCCTGC CCCTTCCCCC TCCCGTTGCT CCTGGTGGCC AGGAAAGCCC TTCACCCCAC
ACAGCTGAGG TGGAGAGTGA GGCCTCACCA CCTCCTGCTC GGCCCCTCCC AGGGGAAGCC
AGGCTGGCGC CCATCTCTGA AGAGGGAAAG CCGCAGCTTG TTGGGCGTTT CCAAGTGACT
TCATCCAAGG AACCGGCTGA GCCTCTTCCC TTGCAGCCAA CATCCCCCAC TCTCTCTGGT
TCTCCAAAAC CTTCAACCCC TCAGCTCACT TCAGAGAGCT CAGATACAGA GGACAGTGCT
GGAGGCGGGC CAGAGACCAG GGAAGCTCTG GCTGAGAGCG ACCGTGCAGC TGAGGGTCTG
GGGGCTGGAG TTGAGGAGGA AGGAGATGAT GGGAAGGAAC CCCAAGTTGG GGGCAGCCCC
CAACCCCTGA GCCATCCCAG CCCAGTGTGG ATGAACTACT CCTACAGCAG CCTGTGTTTG
AGCAGCGAGG AGTCAGAAAG CAGTGGGGAA GATGAGGAGT TCTGGGCTGA GCTGCAGAGT
CTTCGGCAGA AGCACTTGTC AGAGGTGGAA ACACTACAGA CACTACAGAA AAAAGAAATT
GAAGATTTGT ACAGCCGGCT GGGGAAGCAG CCCCCACCGG GTATTGTGGC CCCAGCTGCT
ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC AAGGGCAGCT TCCCCACCTC CCGCCGCAAC
AGCCTACAGC GCTCTGAGCC CCCAGGCCCT GGCATCATGC GAAGGAACTC TCTGAGTGGC
AGCAGCACCG GCTCCCAGGA GCAGCGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011523437.1
CDS999..4688
Misc Feature(1)1512..2294
Misc Feature(2)1533..2294
Misc Feature(3)order(1536..1550,1554..1556,1560..1562,1599..1601,1605..1607,1698..1700,1758..1769,1779..1781,1785..1787,1902..1904,1908..1910,1914..1919,1923..1925,1959..1961,1968..1970,2010..2021)
Misc Feature(4)order(1536..1550,1554..1556,1560..1562,1599..1601,1605..1607,1698..1700,1758..1769,1779..1781,1902..1904,1908..1910,1914..1919,1923..1925,1959..1961)
Misc Feature(5)order(1548..1550,1779..1781,1785..1787,1902..1904,1908..1910,1914..1916,1968..1970,2010..2021)
Misc Feature(6)1956..2021
Misc Feature(7)2355..2465
Translation

Target ORF information:

RefSeq Version XM_011525135.2
Organism Homo sapiens(Human)
Definition Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011525135.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGTTGGCAT CCCCGGCCAC GGAGACCACC GTCCTCATGT CCCAGACTGA GGCCGACCTG 
GCCCTGCGGC CCCCGCCTCC TCTTGGCACC GCGGGGCAGC CCCGCCTCGG GCCCCCTCCT
CGCCGAGCGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC TCGTCTCAGC
CGCCGTAGCT CAGTCGACTT GGGGCTGCTG AGCTCTTGGT CCCTGCCAGC CTCACCCGCT
CCGGACCCCC CCGATCCTCC GGACTCCGCT GGTCCTGGCC CCGCGAGGAG CCCACCGCCT
AGCTCCAAAG AACCCCCCGA GGGCACGTGG ACCGAGGGAG CCCCTGTGAA GGCTGCGGAA
GACTCCGCGC GTCCCGAGCT CCCGGACTCT GCAGTGGGCC CGGGGTCCAG GGAGCCGCTA
AGGGTCCCTG AAGCTGTGGC CCTAGAGCGG CGGCGGGAGC AGGAAGAAAA GGAGGACATG
GAGACCCAGG CTGTGGCAAC GTCCCCCGAT GGCCGATACC TCAAGTTTGA CATCGAGATT
GGACGTGGCT CCTTCAAGAC GGTGTATCGA GGGCTAGACA CCGACACCAC AGTGGAGGTG
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTAGAGCTG AGCGGCAGCG CTTCTCAGAG
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA TGATTCGTGG
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
ACGCTCAAGA CGTACCTGAG GCGGTTCCGG GAGATGAAGC CGCGGGTCCT TCAGCGCTGG
AGCCGCCAAA TCCTGCGGGG ACTTCATTTC CTACACTCCC GGGTTCCTCC CATCCTGCAC
CGGGATCTCA AGTGCGACAA TGTCTTTATC ACGGGACCTA CTGGCTCTGT CAAAATCGGG
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTTGCCA AGAGTGTCAT CGGGACCCCG
GAATTCATGG CCCCCGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
TTCGGCATGT GCATGCTGGA GATGGCCACC TCTGAGTACC CGTACTCCGA GTGCCAGAAT
GCCGCGCAAA TCTACCGCAA GGTCACTTCG GGCAGAAAGC CGAACAGCTT CCACAAGGTG
AAGATACCCG AGGTGAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAACGAGAGG
TTCACCATCC AGGACCTCCT GGCCCACGCC TTCTTCCGCG AGGAGCGCGG TGTGCACGTG
GAACTAGCGG AGGAGGACGA CGGCGAGAAG CCGGGCCTCA AGCTCTGGCT GCGCATGGAG
GACGCGCGGC GCGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCCT GTTCCAGCTG
GGCCGGGACG CGGCCGAGGA GGTGGCACAG GAGATGGTGG CTCTGGGCTT GGTCTGTGAA
GCCGATTACC AGCCAGTGGC CCGTGCAGTA CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
CGTGAGAAGC TGCGTAAAGC AAGGGAATTG GAGGCACTCC CACCAGAGCC AGGACCTCCA
CCAGCAACTG TGCCCATGGC CCCCGGTCCC CCCAGTGTCT TCCCCCCTGA GCCTGAGGAG
CCAGAGGCAG ACCAGCACCA GCCCTTCCTT TTCCGCCACG CCAGCTACTC ATCTACCACT
TCGGATTGCG AGACTGATGG CTACCTCAGC TCCTCCGGCT TCCTGGATGC CTCAGACCCT
GCCCTTCAGC CCCCTGGGGG GGTGCCATCC AGCCTGGCTG AGTCCCATCT CTGCCTGCCC
TCGGCTTTTG CCCTATCCAT TCCACGTTCT GGCCCTGGAA GTGACTTTTC CCCCGGGGAC
AGCTATGCCT CAGATGCAGC TTCAGGCCTT AGCGATGTGG GAGAAGGGAT GGGACAAATG
AGGAGACCCC CAGGGAGGAA TCTCCGGCGC AGACCCCGAT CCCGGCTGCG GGTCACTAGT
GTCTCAGACC AGAATGACAG AGTGGTTGAG TGCCAGCTAC AGACCCATAA CAGCAAGATG
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCGGAAG AGATTGCAGC TGCCATGGTA
TATAACGAGT TCATTCTGCC TTCGGAGCGA GATGGATTTC TCAGACGGAT TCGGGAGATT
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GACACTGGCC CCATGGAGGC TGCTGAAGAC
ACCCTAAGCC CCCAGGAGGA GCCAGCACCA TTACCTGCCC TGCCCGTCCC CCTCCCAGAC
CCATCCAATG AAGAGCTCCA GAGCAGCACC TCCCTGGAGC ACAGGAGCTG GACAGCCTTC
TCCACCTCCT CATCTTCTCC TGGAACTCCT TTGTCTCCTG GAAACCCATT TTCCCCTGGA
ACCCCCATTT CCCCAGGTCC CATCTTCCCC ATCACTTCTC CCCCATGTCA TCCCAGCCCC
TCCCCATTCT CCCCCATTTC TTCCCAGGTC TCCTCAAATC CCTCTCCACA CCCCACCAGC
TCTCCACTTC CATTCTCCTC CAGCACACCC GAGTTTCCGG TCCCACTCTC TCAGTGTCCC
TGGAGTTCTC TCCCCACGAC TTCTCCACCT ACGTTCTCTC CCACTTGTTC TCAGGTCACT
CTTAGTTCCC CTTTCTTTCC TCCGTGCCCC TCCACTTCTT CCTTCCCCTC CACCACAGCA
GCCCCTCTCC TTTCTCTGGC TAGTGCCTTC TCACTGGCTG TGATGACTGT GGCCCAGTCC
CTGCTGTCCC CCTCACCTGG GCTCCTTTCC CAGTCTCCTC CAGCCCCTCC TAGTCCCCTC
CCTAGCCTGC CCCTTCCCCC TCCCGTTGCT CCTGGTGGCC AGGAAAGCCC TTCACCCCAC
ACAGCTGAGG TGGAGAGTGA GGCCTCACCA CCTCCTGCTC GGCCCCTCCC AGGGGAAGCC
AGGCTGGCGC CCATCTCTGA AGAGGGAAAG CCGCAGCTTG TTGGGCGTTT CCAAGTGACT
TCATCCAAGG AACCGGCTGA GCCTCTTCCC TTGCAGCCAA CATCCCCCAC TCTCTCTGGT
TCTCCAAAAC CTTCAACCCC TCAGCTCACT TCAGAGAGCT CAGATACAGA GGACAGTGCT
GGAGGCGGGC CAGAGACCAG GGAAGCTCTG GCTGAGAGCG ACCGTGCAGC TGAGGGTCTG
GGGGCTGGAG TTGAGGAGGA AGGAGATGAT GGGAAGGAAC CCCAAGTTGG GGGCAGCCCC
CAACCCCTGA GCCATCCCAG CCCAGTGTGG ATGAACTACT CCTACAGCAG CCTGTGTTTG
AGCAGCGAGG AGTCAGAAAG CAGTGGGGAA GATGAGGAGT TCTGGGCTGA GCTGCAGAGT
CTTCGGCAGA AGCACTTGTC AGAGGTGGAA ACACTACAGA CACTACAGAA AAAAGAAATT
GAAGATTTGT ACAGCCGGCT GGGGAAGCAG CCCCCACCGG GTATTGTGGC CCCAGCTGCT
ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC AAGGGCAGCT TCCCCACCTC CCGCCGCAAC
AGCCTACAGC GCTCTGAGCC CCCAGGCCCT GGCATCATGC GAAGGAACTC TCTGAGTGGC
AGCAGCACCG GCTCCCAGGA GCAGCGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Impaired degradation of WNK by Akt and PKA phosphorylation of KLHL3.
Biochemical and biophysical research communications467(2)229-34(2015 Nov)
Yoshizaki Y,Mori Y,Tsuzaki Y,Mori T,Nomura N,Wakabayashi M,Takahashi D,Zeniya M,Kikuchi E,Araki Y,Ando F,Isobe K,Nishida H,Ohta A,Susa K,Inoue Y,Chiga M,Rai T,Sasaki S,Uchida S,Sohara E


book

Src-family protein tyrosine kinase phosphorylates WNK4 and modulates its inhibitory effect on KCNJ1 (ROMK).
Proceedings of the National Academy of Sciences of the United States of America112(14)4495-500(2015 Apr)
Lin DH,Yue P,Yarborough O 3rd,Scholl UI,Giebisch G,Lifton RP,Rinehart J,Wang WH


book

Insights in cullin 3/WNK4 and its relationship to blood pressure regulation and electrolyte homeostasis.
Cellular signalling26(6)1166-72(2014 Jun)
Andérica-Romero AC,Escobar L,Padilla-Flores T,Pedraza-Chaverri J


book

WNK4 inhibits plasma membrane targeting of NCC through regulation of syntaxin13 SNARE formation.
Cellular signalling25(12)2469-77(2013 Dec)
Chung WY,Park HW,Han JW,Lee MG,Kim JY


 
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