×

SMARCE1 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol SMARCE1
Entrez Gene ID 6605
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1
Synonyms BAF57,CSS5
General protein information
Preferred Names

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1

Names

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1
BRG1-associated factor 57
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin e1
chromatin remodeling complex BRG1-associated factor 57

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

17

17q21.2

Summary The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart. [provided by RefSeq, Jul 2008].
Disorder MIM:

603111


mRNA and Protein(s)

mRNA Protein Name
NM_003079.4 NP_003070.3 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1



Danio rerio (zebrafish) smarce1 NP_958455.2
Caenorhabditis elegans (roundworm) swsn-3 NP_497613.2
Xenopus tropicalis (tropical clawed frog) smarce1 NP_001005436.1
Macaca mulatta (rhesus monkey) SMARCE1 XP_001099623.2
Homo sapiens (human) SMARCE1 NP_003070.3
Rattus norvegicus (Norway rat) Smarce1 NP_001020164.1
Gallus gallus (chicken) SMARCE1 NP_001006335.2
Canis lupus familiaris (dog) SMARCE1 XP_851010.1
Bos taurus (cattle) SMARCE1 NP_001092586.1
Mus musculus (house mouse) Smarce1 NP_065643.1
Pan troglodytes (chimpanzee) SMARCE1 XP_511478.2
Anopheles gambiae (African malaria mosquito) AgaP_AGAP001994 XP_321065.4


Related articles in PubMed

The developmental and pathogenic roles of BAF57, a special subunit of the BAF chromatin-remodeling complex.
Lomelí H, Castillo-Robles J
FEBS letters590(11)1555-69(2016 Jun)

A heritable form of SMARCE1-related meningiomas with important implications for follow-up and family screening.
Gerkes EH, Fock JM, den Dunnen WF, van Belzen MJ, van der Lans CA, Hoving EW, Fakkert IE, Smith MJ, Evans DG, Olderode-Berends MJ
Neurogenetics17(2)83-9(2016 Apr)

SMARCE1 suppresses EGFR expression and controls responses to MET and ALK inhibitors in lung cancer.
Papadakis AI, Sun C, Knijnenburg TA, Xue Y, Grernrum W, Hölzel M, Nijkamp W, Wessels LF, Beijersbergen RL, Bernards R, Huang S
Cell research25(4)445-58(2015 Apr)

Expression of BAF57 in ovarian cancer cells and drug sensitivity.
Yamaguchi T, Kurita T, Nishio K, Tsukada J, Hachisuga T, Morimoto Y, Iwai Y, Izumi H
Cancer science106(4)359-66(2015 Apr)

Genotype-phenotype correlation of Coffin-Siris syndrome caused by mutations in SMARCB1, SMARCA4, SMARCE1, and ARID1A.
Kosho T, Okamoto N,
American journal of medical genetics. Part C, Seminars in medical genetics166C(3)262-75(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SMARCE1 mutational hits, including novel SMARCE1 mutations, were found in six of eight tested patients with clear cell meningioma
Title: SMARCE1 mutation screening in classification of clear cell meningiomas.

SMARCE1 plays an essential role in breast cancer metastasis by protecting cells against anoikis through the HIF1A/PTK2 pathway.
Title: SMARCE1 regulates metastatic potential of breast cancer cells through the HIF1A/PTK2 pathway.

An exhaustive analysis of the BAF57 molecular and biochemical properties, cellular functions, loss-of-function phenotypes in living organisms and pathological manifestations in cases of human mutations. [review]
Title: The developmental and pathogenic roles of BAF57, a special subunit of the BAF chromatin-remodeling complex.

a family with a pediatric CCM patient and an adult CCM patient and several asymptomatic relatives carrying a germline SMARCE1 mutation.
Title: A heritable form of SMARCE1-related meningiomas with important implications for follow-up and family screening.

Addition of the EGFR inhibitor gefitinib restores the sensitivity of SMARCE1-knockdown cells to MET and ALK inhibitors in NSCLCs. Our findings link SMARCE1 to EGFR oncogenic signaling and suggest targeted treatment options for SMARCE1-deficient tumors.
Title: SMARCE1 suppresses EGFR expression and controls responses to MET and ALK inhibitors in lung cancer.

The following SMARCE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMARCE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
from $49/clone

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu24945 NM_003079.4
Latest version!
Homo sapiens SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 (SMARCE1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu24945
Clone ID Related Accession (Same CDS sequence) NM_003079.4
Accession Version NM_003079.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1236bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Homo sapiens(Human)
Product SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BQ707908.1, AK095047.1 and BC063700.1. This sequence is a reference standard in the RefSeqGene project. On Mar 30, 2004 this sequence version replaced NM_003079.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC069196.1, SRR1803611.146104.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTCAAAAA GACCATCTTA TGCCCCACCT CCCACCCCAG CTCCTGCAAC ACAAATGCCC 
AGCACACCAG GGTTTGTGGG ATACAATCCA TACAGTCATC TCGCCTACAA CAACTACAGG
CTGGGAGGGA ACCCGGGCAC CAACAGCCGG GTCACGGCAT CCTCTGGTAT CACGATTCCA
AAACCCCCAA AGCCACCAGA TAAGCCGCTG ATGCCCTACA TGAGGTACAG CAGAAAGGTC
TGGGACCAAG TAAAGGCTTC CAACCCTGAC CTAAAGTTGT GGGAGATTGG CAAGATTATT
GGTGGCATGT GGCGAGATCT CACTGATGAA GAAAAACAAG AATATTTAAA CGAATACGAA
GCAGAAAAGA TAGAGTACAA TGAATCTATG AAGGCCTATC ATAATTCCCC CGCGTACCTT
GCTTACATAA ATGCAAAAAG TCGTGCAGAA GCTGCTTTAG AGGAAGAAAG TCGACAGAGA
CAATCTCGCA TGGAGAAAGG AGAACCGTAC ATGAGCATTC AGCCTGCTGA AGATCCAGAT
GATTATGATG ATGGCTTTTC AATGAAGCAT ACAGCCACCG CCCGTTTCCA GAGAAACCAC
CGCCTCATCA GTGAAATTCT TAGTGAGAGT GTGGTGCCAG ACGTTCGGTC AGTTGTCACA
ACAGCTAGAA TGCAGGTCCT CAAACGGCAG GTCCAGTCCT TAATGGTTCA TCAGCGAAAA
CTAGAAGCTG AACTTCTTCA AATAGAGGAA CGACACCAGG AGAAGAAGAG GAAATTCCTG
GAAAGCACAG ATTCATTTAA CAATGAACTT AAAAGGTTGT GCGGTCTGAA AGTAGAAGTG
GATATGGAGA AAATTGCAGC TGAGATTGCA CAGGCAGAGG AACAGGCCCG CAAAAGGCAG
GAGGAAAGGG AGAAGGAGGC CGCAGAGCAA GCTGAGCGCA GTCAGAGCAG CATCGTTCCT
GAGGAAGAAC AAGCAGCTAA CAAAGGCGAG GAGAAGAAAG ACGACGAGAA CATTCCGATG
GAGACAGAGG AGACACACCT TGAAGAAACA ACAGAGAGCC AACAGAATGG TGAAGAAGGC
ACGTCTACTC CTGAGGACAA GGAGAGTGGG CAGGAGGGGG TCGACAGTAT GGCAGAGGAA
GGAACCAGTG ATAGTAACAC TGGCTCGGAG AGCAACAGTG CAACAGTGGA GGAGCCACCA
ACAGATCCCA TACCAGAAGA TGAGAAAAAA GAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003070.3
CDS125..1360
Misc Feature(1)113..115
Misc Feature(2)134..136
Misc Feature(3)242..244
Misc Feature(4)332..517
Misc Feature(5)order(332..337,341..346,353..358,365..367,377..379,416..418,425..427,434..436,491..493)
Misc Feature(6)917..919
Exon (1)1..79
Exon (2)80..131
Exon (3)132..175
Exon (4)176..280
Exon (5)281..361
Exon (6)362..493
Exon (7)494..665
Exon (8)666..838
Exon (9)839..940
Exon (10)941..1151
Exon (11)1152..2425
Translation

Target ORF information:

RefSeq Version NM_003079.4
Organism Homo sapiens(Human)
Definition Homo sapiens SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 (SMARCE1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_003079.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTCAAAAA GACCATCTTA TGCCCCACCT CCCACCCCAG CTCCTGCAAC ACAAATGCCC 
AGCACACCAG GGTTTGTGGG ATACAATCCA TACAGTCATC TCGCCTACAA CAACTACAGG
CTGGGAGGGA ACCCGGGCAC CAACAGCCGG GTCACGGCAT CCTCTGGTAT CACGATTCCA
AAACCCCCAA AGCCACCAGA TAAGCCGCTG ATGCCCTACA TGAGGTACAG CAGAAAGGTC
TGGGACCAAG TAAAGGCTTC CAACCCTGAC CTAAAGTTGT GGGAGATTGG CAAGATTATT
GGTGGCATGT GGCGAGATCT CACTGATGAA GAAAAACAAG AATATTTAAA CGAATACGAA
GCAGAAAAGA TAGAGTACAA TGAATCTATG AAGGCCTATC ATAATTCCCC CGCGTACCTT
GCTTACATAA ATGCAAAAAG TCGTGCAGAA GCTGCTTTAG AGGAAGAAAG TCGACAGAGA
CAATCTCGCA TGGAGAAAGG AGAACCGTAC ATGAGCATTC AGCCTGCTGA AGATCCAGAT
GATTATGATG ATGGCTTTTC AATGAAGCAT ACAGCCACCG CCCGTTTCCA GAGAAACCAC
CGCCTCATCA GTGAAATTCT TAGTGAGAGT GTGGTGCCAG ACGTTCGGTC AGTTGTCACA
ACAGCTAGAA TGCAGGTCCT CAAACGGCAG GTCCAGTCCT TAATGGTTCA TCAGCGAAAA
CTAGAAGCTG AACTTCTTCA AATAGAGGAA CGACACCAGG AGAAGAAGAG GAAATTCCTG
GAAAGCACAG ATTCATTTAA CAATGAACTT AAAAGGTTGT GCGGTCTGAA AGTAGAAGTG
GATATGGAGA AAATTGCAGC TGAGATTGCA CAGGCAGAGG AACAGGCCCG CAAAAGGCAG
GAGGAAAGGG AGAAGGAGGC CGCAGAGCAA GCTGAGCGCA GTCAGAGCAG CATCGTTCCT
GAGGAAGAAC AAGCAGCTAA CAAAGGCGAG GAGAAGAAAG ACGACGAGAA CATTCCGATG
GAGACAGAGG AGACACACCT TGAAGAAACA ACAGAGAGCC AACAGAATGG TGAAGAAGGC
ACGTCTACTC CTGAGGACAA GGAGAGTGGG CAGGAGGGGG TCGACAGTAT GGCAGAGGAA
GGAACCAGTG ATAGTAACAC TGGCTCGGAG AGCAACAGTG CAACAGTGGA GGAGCCACCA
ACAGATCCCA TACCAGAAGA TGAGAAAAAA GAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

The developmental and pathogenic roles of BAF57, a special subunit of the BAF chromatin-remodeling complex.
FEBS letters590(11)1555-69(2016 Jun)
Lomelí H,Castillo-Robles J


book

A heritable form of SMARCE1-related meningiomas with important implications for follow-up and family screening.
Neurogenetics17(2)83-9(2016 Apr)
Gerkes EH,Fock JM,den Dunnen WF,van Belzen MJ,van der Lans CA,Hoving EW,Fakkert IE,Smith MJ,Evans DG,Olderode-Berends MJ


book

SMARCE1 suppresses EGFR expression and controls responses to MET and ALK inhibitors in lung cancer.
Cell research25(4)445-58(2015 Apr)
Papadakis AI,Sun C,Knijnenburg TA,Xue Y,Grernrum W,Hölzel M,Nijkamp W,Wessels LF,Beijersbergen RL,Bernards R,Huang S


book

Expression of BAF57 in ovarian cancer cells and drug sensitivity.
Cancer science106(4)359-66(2015 Apr)
Yamaguchi T,Kurita T,Nishio K,Tsukada J,Hachisuga T,Morimoto Y,Iwai Y,Izumi H


book

Genotype-phenotype correlation of Coffin-Siris syndrome caused by mutations in SMARCB1, SMARCA4, SMARCE1, and ARID1A.
American journal of medical genetics. Part C, Seminars in medical genetics166C(3)262-75(2014 Sep)
Kosho T,Okamoto N,


 
*
*
*
*