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SMPD1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol SMPD1
Entrez Gene ID 6609
Full Name sphingomyelin phosphodiesterase 1, acid lysosomal
Synonyms ASM, ASMASE, NPD
General protein information
Preferred Names
sphingomyelin phosphodiesterase
Names
sphingomyelin phosphodiesterase
acid sphingomyelinase
NP_000534.3
NP_001007594.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11p15.4-p15.1

Summary The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2010]. lac of sum
Disorder MIM:

607608

Disorder Html: Niemann-Pick disease, type A, 257200 (3); Niemann-Pick disease, type

mRNA and Protein(s)

mRNA Protein Name
XM_011520303 XP_011518605 sphingomyelin phosphodiesterase isoform X1
XM_005253075 XP_005253132 sphingomyelin phosphodiesterase isoform X2
XM_011520304 XP_011518606 sphingomyelin phosphodiesterase isoform X3
NM_000543 NP_000534 sphingomyelin phosphodiesterase isoform 1 precursor
NM_001007593 NP_001007594 sphingomyelin phosphodiesterase isoform 2 precursor



Homo sapiens (human) SMPD1 NP_000534.3
Pan troglodytes (chimpanzee) SMPD1 XP_508253.2
Macaca mulatta (Rhesus monkey) SMPD1 XP_001110212.1
Canis lupus familiaris (dog) SMPD1 XP_542452.1
Bos taurus (cattle) SMPD1 NP_001068655.1
Mus musculus (house mouse) Smpd1 NP_035551.1
Rattus norvegicus (Norway rat) Smpd1 NP_001006998.1
Gallus gallus (chicken) SMPD1 XP_003640663.2
Danio rerio (zebrafish) smpd1 XP_683907.1
Drosophila melanogaster (fruit fly) CG3376 NP_611904.1
Caenorhabditis elegans asm-2 NP_509894.2
Xenopus (Silurana) tropicalis (western clawed frog) LOC100487433 XP_002943546.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SMPD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMPD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu60647 XM_011520303 PREDICTED: Homo sapiens sphingomyelin phosphodiesterase 1, acid lysosomal (SMPD1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu44952 XM_005253075 PREDICTED: Homo sapiens sphingomyelin phosphodiesterase 1, acid lysosomal (SMPD1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $307.30
$439.00
OHu60648 XM_011520304 PREDICTED: Homo sapiens sphingomyelin phosphodiesterase 1, acid lysosomal (SMPD1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu18710
NM_000543 Homo sapiens sphingomyelin phosphodiesterase 1, acid lysosomal (SMPD1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu16724 NM_001007593 Homo sapiens sphingomyelin phosphodiesterase 1, acid lysosomal (SMPD1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $307.30
$439.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu60647
Clone ID Related Accession (Same CDS sequence) XM_011520303
Accession Version XM_011520303.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1764bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product sphingomyelin phosphodiesterase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009237.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
ATCAACCTCG GGCTGAAGAA GGAACCCAAT GTGGCTCGCG TGGGCTCCGT GGCCATCAAG
CTGTGCAATC TGCTGAAGAT AGCACCACCT GCCGTGTGCC AATCCATTGT CCACCTCTTT
GAGGATGACA TGGTGGAGGT GTGGAGACGC TCAGTGCTGA GCCCATCTGA GGCCTGTGGC
CTGCTCCTGG GCTCCACCTG TGGGCACTGG GACATTTTCT CATCTTGGAA CATCTCTTTG
CCTACTGTGC CGAAGCCGCC CCCCAAACCC CCTAGCCCCC CAGCCCCAGG TGCCCCTGTC
AGCCGCATCC TCTTCCTCAC TGACCTGCAC TGGGATCATG ACTACCTGGA GGGCACGGAC
CCTGACTGTG CAGACCCACT GTGCTGCCGC CGGGGTTCTG GCCTGCCGCC CGCATCCCGG
CCAGGTGCCG GATACTGGGG CGAATACAGC AAGTGTGACC TGCCCCTGAG GACCCTGGAG
AGCCTGTTGA GTGGGCTGGG CCCAGCCGGC CCTTTTGATA TGGTGTACTG GACAGGAGAC
ATCCCCGCAC ATGATGTCTG GCACCAGACT CGTCAGGACC AACTGCGGGC CCTGACCACC
GTCACAGCAC TTGTGAGGAA GTTCCTGGGG CCAGTGCCAG TGTACCCTGC TGTGGGTAAC
CATGAAAGCA CACCTGTCAA TAGCTTCCCT CCCCCCTTCA TTGAGGGCAA CCACTCCTCC
CGCTGGCTCT ATGAAGCGAT GGCCAAGGCT TGGGAGCCCT GGCTGCCTGC CGAAGCCCTG
CGCACCCTCA GGTACTTATC GTCCGTGGAA ACCCAGGAAG GGAAAAGAAA GGTGCATATA
ATTGGCCACA TTCCCCCAGG GCACTGTCTG AAGAGCTGGA GCTGGAATTA TTACCGAATT
GTAGCCAGGT ATGAGAACAC CCTGGCTGCT CAGTTCTTTG GCCACACTCA TGTGGATGAA
TTTGAGGTCT TCTATGATGA AGAGACTCTG AGCCGGCCGC TGGCTGTAGC CTTCCTGGCA
CCCAGTGCAA CTACCTACAT CGGCCTTAAT CCTGGTTACC GTGTGTACCA AATAGATGGA
AACTACTCCG GGAGCTCTCA CGTGGTCCTG GACCATGAGA CCTACATCCT GAATCTGACC
CAGGCAAACA TACCGGGAGC CATACCGCAC TGGCAGCTTC TCTACAGGGC TCGAGAAACC
TATGGGCTGC CCAACACACT GCCTACCGCC TGGCACAACC TGGTATATCG CATGCGGGGC
GACATGCAAC TTTTCCAGAC CTTCTGGTTT CTCTACCATA AGGGCCACCC ACCCTCGGAG
CCCTGTGGCA CGCCCTGCCG TCTGGCTACT CTTTGTGCCC AGCTCTCTGC CCGTGCTGAC
AGCCCTGCTC TGTGCCGCCA CCTGATGCCA GATGGGAGCC TCCCAGAGGC CCAGAGCCTG
TGGCCAAGGC CACTGTTTTG CTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011518605.1
CDS299..2062
Misc Feature(1)563..784(+)
Misc Feature(2)905..1660(+)
Misc Feature(3)920..1549(+)
Misc Feature(4)920..1549(+)
Translation

Target ORF information:

RefSeq Version XM_011520303
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens sphingomyelin phosphodiesterase 1, acid lysosomal (SMPD1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011520303

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
ATCAACCTCG GGCTGAAGAA GGAACCCAAT GTGGCTCGCG TGGGCTCCGT GGCCATCAAG
CTGTGCAATC TGCTGAAGAT AGCACCACCT GCCGTGTGCC AATCCATTGT CCACCTCTTT
GAGGATGACA TGGTGGAGGT GTGGAGACGC TCAGTGCTGA GCCCATCTGA GGCCTGTGGC
CTGCTCCTGG GCTCCACCTG TGGGCACTGG GACATTTTCT CATCTTGGAA CATCTCTTTG
CCTACTGTGC CGAAGCCGCC CCCCAAACCC CCTAGCCCCC CAGCCCCAGG TGCCCCTGTC
AGCCGCATCC TCTTCCTCAC TGACCTGCAC TGGGATCATG ACTACCTGGA GGGCACGGAC
CCTGACTGTG CAGACCCACT GTGCTGCCGC CGGGGTTCTG GCCTGCCGCC CGCATCCCGG
CCAGGTGCCG GATACTGGGG CGAATACAGC AAGTGTGACC TGCCCCTGAG GACCCTGGAG
AGCCTGTTGA GTGGGCTGGG CCCAGCCGGC CCTTTTGATA TGGTGTACTG GACAGGAGAC
ATCCCCGCAC ATGATGTCTG GCACCAGACT CGTCAGGACC AACTGCGGGC CCTGACCACC
GTCACAGCAC TTGTGAGGAA GTTCCTGGGG CCAGTGCCAG TGTACCCTGC TGTGGGTAAC
CATGAAAGCA CACCTGTCAA TAGCTTCCCT CCCCCCTTCA TTGAGGGCAA CCACTCCTCC
CGCTGGCTCT ATGAAGCGAT GGCCAAGGCT TGGGAGCCCT GGCTGCCTGC CGAAGCCCTG
CGCACCCTCA GGTACTTATC GTCCGTGGAA ACCCAGGAAG GGAAAAGAAA GGTGCATATA
ATTGGCCACA TTCCCCCAGG GCACTGTCTG AAGAGCTGGA GCTGGAATTA TTACCGAATT
GTAGCCAGGT ATGAGAACAC CCTGGCTGCT CAGTTCTTTG GCCACACTCA TGTGGATGAA
TTTGAGGTCT TCTATGATGA AGAGACTCTG AGCCGGCCGC TGGCTGTAGC CTTCCTGGCA
CCCAGTGCAA CTACCTACAT CGGCCTTAAT CCTGGTTACC GTGTGTACCA AATAGATGGA
AACTACTCCG GGAGCTCTCA CGTGGTCCTG GACCATGAGA CCTACATCCT GAATCTGACC
CAGGCAAACA TACCGGGAGC CATACCGCAC TGGCAGCTTC TCTACAGGGC TCGAGAAACC
TATGGGCTGC CCAACACACT GCCTACCGCC TGGCACAACC TGGTATATCG CATGCGGGGC
GACATGCAAC TTTTCCAGAC CTTCTGGTTT CTCTACCATA AGGGCCACCC ACCCTCGGAG
CCCTGTGGCA CGCCCTGCCG TCTGGCTACT CTTTGTGCCC AGCTCTCTGC CCGTGCTGAC
AGCCCTGCTC TGTGCCGCCA CCTGATGCCA GATGGGAGCC TCCCAGAGGC CCAGAGCCTG
TGGCCAAGGC CACTGTTTTG CTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu44952
Clone ID Related Accession (Same CDS sequence) XM_005253075
Accession Version XM_005253075.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1527bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product sphingomyelin phosphodiesterase isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009237.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578820705. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
ATCAACCTCG GGCTGAAGAA GGAACCCAAT GTGGCTCGCG TGGGCTCCGT GGCCATCAAG
CTGTGCAATC TGCTGAAGAT AGCACCACCT GCCGTGTGCC AATCCATTGT CCACCTCTTT
GAGGATGACA TGGTGGAGGT GTGGAGACGC TCAGTGCTGA GCCCATCTGA GGCCTGTGGC
CTGCTCCTGG GCTCCACCTG TGGGCACTGG GACATTTTCT CATCTTGGAA CATCTCTTTG
CCTACTGTGC CGAAGCCGCC CCCCAAACCC CCTAGCCCCC CAGCCCCAGG TGCCCCTGTC
AGCCGCATCC TCTTCCTCAC TGACCTGCAC TGGGATCATG ACTACCTGGA GGGCACGGAC
CCTGACTGTG CAGACCCACT GTGCTGCCGC CGGGGTTCTG GCCTGCCGCC CGCATCCCGG
CCAGGTGCCG GATACTGGGG CGAATACAGC AAGTGTGACC TGCCCCTGAG GACCCTGGAG
AGCCTGTTGA GTGGGCTGGG CCCAGCCGGC CCTTTTGATA TGGTGTACTG GACAGGAGAC
ATCCCCGCAC ATGATGTCTG GCACCAGACT CGTCAGGACC AACTGCGGGC CCTGACCACC
GTCACAGCAC TTGTGAGGAA GTTCCTGGGG CCAGTGCCAG TGTACCCTGC TGTGGGTAAC
CATGAAAGCA CACCTGTCAA TAGCTTCCCT CCCCCCTTCA TTGAGGGCAA CCACTCCTCC
CGCTGGCTCT ATGAAGCGAT GGCCAAGGCT TGGGAGCCCT GGCTGCCTGC CGAAGCCCTG
CGCACCCTCA GAATTGGGGG GTTCTATGCT CTTTCCCCAT ACCCCGGTCT CCGCCTCATC
TCTCTCAATA TGAATTTTTG TTCCCGTGAG AACTTCTGGC TCTTGATCAA CTCCACGGAT
CCCGCAGGAC AGCTCCAGTG GCTGGTGGGG GAGCTTCAGG CTGCTGAGGA TCGAGGAGAC
AAAGTGCATA TAATTGGCCA CATTCCCCCA GGGCACTGTC TGAAGAGCTG GAGCTGGAAT
TATTACCGAA TTGTAGCCAG GTATGAGAAC ACCCTGGCTG CTCAGTTCTT TGGCCACACT
CATGTGGATG AATTTGAGGT CTTCTATGAT GAAGAGACTC TGAGCCGGCC GCTGGCTGTA
GCCTTCCTGG CACCCAGTGC AACTACCTAC ATCGGCCTTA ATCCTGTCAG CCCCACATCC
TTGCAGGTTA CCGTGTGTAC CAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005253132.1
CDS299..1825
Misc Feature(1)563..784(+)
Misc Feature(2)905..1783(+)
Misc Feature(3)920..1681(+)
Misc Feature(4)920..1681(+)
Misc Feature(5)1070..>1687(+)
Translation

Target ORF information:

RefSeq Version XM_005253075
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens sphingomyelin phosphodiesterase 1, acid lysosomal (SMPD1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005253075

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
ATCAACCTCG GGCTGAAGAA GGAACCCAAT GTGGCTCGCG TGGGCTCCGT GGCCATCAAG
CTGTGCAATC TGCTGAAGAT AGCACCACCT GCCGTGTGCC AATCCATTGT CCACCTCTTT
GAGGATGACA TGGTGGAGGT GTGGAGACGC TCAGTGCTGA GCCCATCTGA GGCCTGTGGC
CTGCTCCTGG GCTCCACCTG TGGGCACTGG GACATTTTCT CATCTTGGAA CATCTCTTTG
CCTACTGTGC CGAAGCCGCC CCCCAAACCC CCTAGCCCCC CAGCCCCAGG TGCCCCTGTC
AGCCGCATCC TCTTCCTCAC TGACCTGCAC TGGGATCATG ACTACCTGGA GGGCACGGAC
CCTGACTGTG CAGACCCACT GTGCTGCCGC CGGGGTTCTG GCCTGCCGCC CGCATCCCGG
CCAGGTGCCG GATACTGGGG CGAATACAGC AAGTGTGACC TGCCCCTGAG GACCCTGGAG
AGCCTGTTGA GTGGGCTGGG CCCAGCCGGC CCTTTTGATA TGGTGTACTG GACAGGAGAC
ATCCCCGCAC ATGATGTCTG GCACCAGACT CGTCAGGACC AACTGCGGGC CCTGACCACC
GTCACAGCAC TTGTGAGGAA GTTCCTGGGG CCAGTGCCAG TGTACCCTGC TGTGGGTAAC
CATGAAAGCA CACCTGTCAA TAGCTTCCCT CCCCCCTTCA TTGAGGGCAA CCACTCCTCC
CGCTGGCTCT ATGAAGCGAT GGCCAAGGCT TGGGAGCCCT GGCTGCCTGC CGAAGCCCTG
CGCACCCTCA GAATTGGGGG GTTCTATGCT CTTTCCCCAT ACCCCGGTCT CCGCCTCATC
TCTCTCAATA TGAATTTTTG TTCCCGTGAG AACTTCTGGC TCTTGATCAA CTCCACGGAT
CCCGCAGGAC AGCTCCAGTG GCTGGTGGGG GAGCTTCAGG CTGCTGAGGA TCGAGGAGAC
AAAGTGCATA TAATTGGCCA CATTCCCCCA GGGCACTGTC TGAAGAGCTG GAGCTGGAAT
TATTACCGAA TTGTAGCCAG GTATGAGAAC ACCCTGGCTG CTCAGTTCTT TGGCCACACT
CATGTGGATG AATTTGAGGT CTTCTATGAT GAAGAGACTC TGAGCCGGCC GCTGGCTGTA
GCCTTCCTGG CACCCAGTGC AACTACCTAC ATCGGCCTTA ATCCTGTCAG CCCCACATCC
TTGCAGGTTA CCGTGTGTAC CAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60648
Clone ID Related Accession (Same CDS sequence) XM_011520304
Accession Version XM_011520304.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1395bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product sphingomyelin phosphodiesterase isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009237.19) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
ATCAACCTCG GGCTGAAGAA GGAACCCAAT GTGGCTCGCG TGGGCTCCGT GGCCATCAAG
CTGTGCAATC TGCTGAAGAT AGCACCACCT GCCGTGTGCC AATCCATTGT CCACCTCTTT
GAGGATGACA TGGTGGAGGT GTGGAGACGC TCAGTGCTGA GCCCATCTGA GGCCTGTGGC
CTGCTCCTGG GCTCCACCTG TGGGCACTGG GACATTTTCT CATCTTGGAA CATCTCTTTG
CCTACTGTGC CGAAGCCGCC CCCCAAACCC CCTAGCCCCC CAGCCCCAGG TGCCCCTGTC
AGCCGCATCC TCTTCCTCAC TGACCTGCAC TGGGATCATG ACTACCTGGA GGGCACGGAC
CCTGACTGTG CAGACCCACT GTGCTGCCGC CGGGGTTCTG GCCTGCCGCC CGCATCCCGG
CCAGGTGCCG GATACTGGGG CGAATACAGC AAGTGTGACC TGCCCCTGAG GACCCTGGAG
AGCCTGTTGA GTGGGCTGGG CCCAGCCGGC CCTTTTGATA TGGTGTACTG GACAGGAGAC
ATCCCCGCAC ATGATGTCTG GCACCAGACT CGTCAGGACC AACTGCGGGC CCTGACCACC
GTCACAGCAC TTGTGAGGAA GTTCCTGGGG CCAGTGCCAG TGTACCCTGC TGTGGGTAAC
CATGAAAGCA CACCTGTCAA TAGCTTCCCT CCCCCCTTCA TTGAGGGCAA CCACTCCTCC
CGCTGGCTCT ATGAAGCGAT GGCCAAGGCT TGGGAGCCCT GGCTGCCTGC CGAAGCCCTG
CGCACCCTCA GGTACTTATC GTCCGTGGAA ACCCAGGAAG GGAAAAGAAA GGTGCATATA
ATTGGCCACA TTCCCCCAGG GCACTGTCTG AAGAGCTGGA GCTGGAATTA TTACCGAATT
GTAGCCAGGT ATGAGAACAC CCTGGCTGCT CAGTTCTTTG GCCACACTCA TGTGGATGAA
TTTGAGGTCT TCTATGATGA AGAGACTCTG AGCCGGCCGC TGGCTGTAGC CTTCCTGGCA
CCCAGTGCAA CTACCTACAT CGGCCTTAAT CCTGTCAGCC CCACATCCTT GCAGGTTACC
GTGTGTACCA AATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011518606.1
CDS299..1693
Misc Feature(1)563..784(+)
Misc Feature(2)905..1651(+)
Misc Feature(3)920..1549(+)
Misc Feature(4)920..1549(+)
Translation

Target ORF information:

RefSeq Version XM_011520304
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens sphingomyelin phosphodiesterase 1, acid lysosomal (SMPD1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011520304

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
ATCAACCTCG GGCTGAAGAA GGAACCCAAT GTGGCTCGCG TGGGCTCCGT GGCCATCAAG
CTGTGCAATC TGCTGAAGAT AGCACCACCT GCCGTGTGCC AATCCATTGT CCACCTCTTT
GAGGATGACA TGGTGGAGGT GTGGAGACGC TCAGTGCTGA GCCCATCTGA GGCCTGTGGC
CTGCTCCTGG GCTCCACCTG TGGGCACTGG GACATTTTCT CATCTTGGAA CATCTCTTTG
CCTACTGTGC CGAAGCCGCC CCCCAAACCC CCTAGCCCCC CAGCCCCAGG TGCCCCTGTC
AGCCGCATCC TCTTCCTCAC TGACCTGCAC TGGGATCATG ACTACCTGGA GGGCACGGAC
CCTGACTGTG CAGACCCACT GTGCTGCCGC CGGGGTTCTG GCCTGCCGCC CGCATCCCGG
CCAGGTGCCG GATACTGGGG CGAATACAGC AAGTGTGACC TGCCCCTGAG GACCCTGGAG
AGCCTGTTGA GTGGGCTGGG CCCAGCCGGC CCTTTTGATA TGGTGTACTG GACAGGAGAC
ATCCCCGCAC ATGATGTCTG GCACCAGACT CGTCAGGACC AACTGCGGGC CCTGACCACC
GTCACAGCAC TTGTGAGGAA GTTCCTGGGG CCAGTGCCAG TGTACCCTGC TGTGGGTAAC
CATGAAAGCA CACCTGTCAA TAGCTTCCCT CCCCCCTTCA TTGAGGGCAA CCACTCCTCC
CGCTGGCTCT ATGAAGCGAT GGCCAAGGCT TGGGAGCCCT GGCTGCCTGC CGAAGCCCTG
CGCACCCTCA GGTACTTATC GTCCGTGGAA ACCCAGGAAG GGAAAAGAAA GGTGCATATA
ATTGGCCACA TTCCCCCAGG GCACTGTCTG AAGAGCTGGA GCTGGAATTA TTACCGAATT
GTAGCCAGGT ATGAGAACAC CCTGGCTGCT CAGTTCTTTG GCCACACTCA TGTGGATGAA
TTTGAGGTCT TCTATGATGA AGAGACTCTG AGCCGGCCGC TGGCTGTAGC CTTCCTGGCA
CCCAGTGCAA CTACCTACAT CGGCCTTAAT CCTGTCAGCC CCACATCCTT GCAGGTTACC
GTGTGTACCA AATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18710
Clone ID Related Accession (Same CDS sequence) NM_000543
Accession Version NM_000543.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1896bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product sphingomyelin phosphodiesterase isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI599208.1, AB209775.1, M59916.1 and AA746054.1. This sequence is a reference standard in the RefSeqGene project. On Jul 17, 2010 this sequence version replaced gi:56117839. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB209775.1, X59960.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
ATCAACCTCG GGCTGAAGAA GGAACCCAAT GTGGCTCGCG TGGGCTCCGT GGCCATCAAG
CTGTGCAATC TGCTGAAGAT AGCACCACCT GCCGTGTGCC AATCCATTGT CCACCTCTTT
GAGGATGACA TGGTGGAGGT GTGGAGACGC TCAGTGCTGA GCCCATCTGA GGCCTGTGGC
CTGCTCCTGG GCTCCACCTG TGGGCACTGG GACATTTTCT CATCTTGGAA CATCTCTTTG
CCTACTGTGC CGAAGCCGCC CCCCAAACCC CCTAGCCCCC CAGCCCCAGG TGCCCCTGTC
AGCCGCATCC TCTTCCTCAC TGACCTGCAC TGGGATCATG ACTACCTGGA GGGCACGGAC
CCTGACTGTG CAGACCCACT GTGCTGCCGC CGGGGTTCTG GCCTGCCGCC CGCATCCCGG
CCAGGTGCCG GATACTGGGG CGAATACAGC AAGTGTGACC TGCCCCTGAG GACCCTGGAG
AGCCTGTTGA GTGGGCTGGG CCCAGCCGGC CCTTTTGATA TGGTGTACTG GACAGGAGAC
ATCCCCGCAC ATGATGTCTG GCACCAGACT CGTCAGGACC AACTGCGGGC CCTGACCACC
GTCACAGCAC TTGTGAGGAA GTTCCTGGGG CCAGTGCCAG TGTACCCTGC TGTGGGTAAC
CATGAAAGCA CACCTGTCAA TAGCTTCCCT CCCCCCTTCA TTGAGGGCAA CCACTCCTCC
CGCTGGCTCT ATGAAGCGAT GGCCAAGGCT TGGGAGCCCT GGCTGCCTGC CGAAGCCCTG
CGCACCCTCA GAATTGGGGG GTTCTATGCT CTTTCCCCAT ACCCCGGTCT CCGCCTCATC
TCTCTCAATA TGAATTTTTG TTCCCGTGAG AACTTCTGGC TCTTGATCAA CTCCACGGAT
CCCGCAGGAC AGCTCCAGTG GCTGGTGGGG GAGCTTCAGG CTGCTGAGGA TCGAGGAGAC
AAAGTGCATA TAATTGGCCA CATTCCCCCA GGGCACTGTC TGAAGAGCTG GAGCTGGAAT
TATTACCGAA TTGTAGCCAG GTATGAGAAC ACCCTGGCTG CTCAGTTCTT TGGCCACACT
CATGTGGATG AATTTGAGGT CTTCTATGAT GAAGAGACTC TGAGCCGGCC GCTGGCTGTA
GCCTTCCTGG CACCCAGTGC AACTACCTAC ATCGGCCTTA ATCCTGGTTA CCGTGTGTAC
CAAATAGATG GAAACTACTC CGGGAGCTCT CACGTGGTCC TGGACCATGA GACCTACATC
CTGAATCTGA CCCAGGCAAA CATACCGGGA GCCATACCGC ACTGGCAGCT TCTCTACAGG
GCTCGAGAAA CCTATGGGCT GCCCAACACA CTGCCTACCG CCTGGCACAA CCTGGTATAT
CGCATGCGGG GCGACATGCA ACTTTTCCAG ACCTTCTGGT TTCTCTACCA TAAGGGCCAC
CCACCCTCGG AGCCCTGTGG CACGCCCTGC CGTCTGGCTA CTCTTTGTGC CCAGCTCTCT
GCCCGTGCTG ACAGCCCTGC TCTGTGCCGC CACCTGATGC CAGATGGGAG CCTCCCAGAG
GCCCAGAGCC TGTGGCCAAG GCCACTGTTT TGCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000534.3
CDS186..2081
Misc Feature(1)84..86(+)
Misc Feature(2)447..449(+)
Misc Feature(3)450..671(+)
Misc Feature(4)531..533(+)
Misc Feature(5)549..584(+)
Misc Feature(6)792..1679(+)
Misc Feature(7)807..1568(+)
Misc Feature(8)807..1568(+)
Misc Feature(9)852..869(+)
Misc Feature(10)870..941(+)
Misc Feature(11)957..1748(+)
Misc Feature(12)1194..1196(+)
Misc Feature(13)1344..1484(+)
Misc Feature(14)1941..1955(+)
Misc Feature(15)1971..2012(+)
Exon (1)1..503
Gene:SMPD1
Gene Synonym:
Exon (2)504..1276
Gene:SMPD1
Gene Synonym:
Exon (3)1277..1448
Gene:SMPD1
Gene Synonym:
Exon (4)1449..1525
Gene:SMPD1
Gene Synonym:
Exon (5)1526..1671
Gene:SMPD1
Gene Synonym:
Exon (6)1672..2472
Gene:SMPD1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000543
Organism Homo sapiens (human)
Definition Homo sapiens sphingomyelin phosphodiesterase 1, acid lysosomal (SMPD1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000543

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
ATCAACCTCG GGCTGAAGAA GGAACCCAAT GTGGCTCGCG TGGGCTCCGT GGCCATCAAG
CTGTGCAATC TGCTGAAGAT AGCACCACCT GCCGTGTGCC AATCCATTGT CCACCTCTTT
GAGGATGACA TGGTGGAGGT GTGGAGACGC TCAGTGCTGA GCCCATCTGA GGCCTGTGGC
CTGCTCCTGG GCTCCACCTG TGGGCACTGG GACATTTTCT CATCTTGGAA CATCTCTTTG
CCTACTGTGC CGAAGCCGCC CCCCAAACCC CCTAGCCCCC CAGCCCCAGG TGCCCCTGTC
AGCCGCATCC TCTTCCTCAC TGACCTGCAC TGGGATCATG ACTACCTGGA GGGCACGGAC
CCTGACTGTG CAGACCCACT GTGCTGCCGC CGGGGTTCTG GCCTGCCGCC CGCATCCCGG
CCAGGTGCCG GATACTGGGG CGAATACAGC AAGTGTGACC TGCCCCTGAG GACCCTGGAG
AGCCTGTTGA GTGGGCTGGG CCCAGCCGGC CCTTTTGATA TGGTGTACTG GACAGGAGAC
ATCCCCGCAC ATGATGTCTG GCACCAGACT CGTCAGGACC AACTGCGGGC CCTGACCACC
GTCACAGCAC TTGTGAGGAA GTTCCTGGGG CCAGTGCCAG TGTACCCTGC TGTGGGTAAC
CATGAAAGCA CACCTGTCAA TAGCTTCCCT CCCCCCTTCA TTGAGGGCAA CCACTCCTCC
CGCTGGCTCT ATGAAGCGAT GGCCAAGGCT TGGGAGCCCT GGCTGCCTGC CGAAGCCCTG
CGCACCCTCA GAATTGGGGG GTTCTATGCT CTTTCCCCAT ACCCCGGTCT CCGCCTCATC
TCTCTCAATA TGAATTTTTG TTCCCGTGAG AACTTCTGGC TCTTGATCAA CTCCACGGAT
CCCGCAGGAC AGCTCCAGTG GCTGGTGGGG GAGCTTCAGG CTGCTGAGGA TCGAGGAGAC
AAAGTGCATA TAATTGGCCA CATTCCCCCA GGGCACTGTC TGAAGAGCTG GAGCTGGAAT
TATTACCGAA TTGTAGCCAG GTATGAGAAC ACCCTGGCTG CTCAGTTCTT TGGCCACACT
CATGTGGATG AATTTGAGGT CTTCTATGAT GAAGAGACTC TGAGCCGGCC GCTGGCTGTA
GCCTTCCTGG CACCCAGTGC AACTACCTAC ATCGGCCTTA ATCCTGGTTA CCGTGTGTAC
CAAATAGATG GAAACTACTC CGGGAGCTCT CACGTGGTCC TGGACCATGA GACCTACATC
CTGAATCTGA CCCAGGCAAA CATACCGGGA GCCATACCGC ACTGGCAGCT TCTCTACAGG
GCTCGAGAAA CCTATGGGCT GCCCAACACA CTGCCTACCG CCTGGCACAA CCTGGTATAT
CGCATGCGGG GCGACATGCA ACTTTTCCAG ACCTTCTGGT TTCTCTACCA TAAGGGCCAC
CCACCCTCGG AGCCCTGTGG CACGCCCTGC CGTCTGGCTA CTCTTTGTGC CCAGCTCTCT
GCCCGTGCTG ACAGCCCTGC TCTGTGCCGC CACCTGATGC CAGATGGGAG CCTCCCAGAG
GCCCAGAGCC TGTGGCCAAG GCCACTGTTT TGCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16724
Clone ID Related Accession (Same CDS sequence) NM_001007593
Accession Version NM_001007593.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1893bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product sphingomyelin phosphodiesterase isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI599208.1, AB209775.1, M59916.1 and AA746054.1. On Jul 17, 2010 this sequence version replaced gi:56117841. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The encoded protein (isoform 2) is one amino acid shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292388.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142363 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
ATCAACCTCG GGCTGAAGGA ACCCAATGTG GCTCGCGTGG GCTCCGTGGC CATCAAGCTG
TGCAATCTGC TGAAGATAGC ACCACCTGCC GTGTGCCAAT CCATTGTCCA CCTCTTTGAG
GATGACATGG TGGAGGTGTG GAGACGCTCA GTGCTGAGCC CATCTGAGGC CTGTGGCCTG
CTCCTGGGCT CCACCTGTGG GCACTGGGAC ATTTTCTCAT CTTGGAACAT CTCTTTGCCT
ACTGTGCCGA AGCCGCCCCC CAAACCCCCT AGCCCCCCAG CCCCAGGTGC CCCTGTCAGC
CGCATCCTCT TCCTCACTGA CCTGCACTGG GATCATGACT ACCTGGAGGG CACGGACCCT
GACTGTGCAG ACCCACTGTG CTGCCGCCGG GGTTCTGGCC TGCCGCCCGC ATCCCGGCCA
GGTGCCGGAT ACTGGGGCGA ATACAGCAAG TGTGACCTGC CCCTGAGGAC CCTGGAGAGC
CTGTTGAGTG GGCTGGGCCC AGCCGGCCCT TTTGATATGG TGTACTGGAC AGGAGACATC
CCCGCACATG ATGTCTGGCA CCAGACTCGT CAGGACCAAC TGCGGGCCCT GACCACCGTC
ACAGCACTTG TGAGGAAGTT CCTGGGGCCA GTGCCAGTGT ACCCTGCTGT GGGTAACCAT
GAAAGCACAC CTGTCAATAG CTTCCCTCCC CCCTTCATTG AGGGCAACCA CTCCTCCCGC
TGGCTCTATG AAGCGATGGC CAAGGCTTGG GAGCCCTGGC TGCCTGCCGA AGCCCTGCGC
ACCCTCAGAA TTGGGGGGTT CTATGCTCTT TCCCCATACC CCGGTCTCCG CCTCATCTCT
CTCAATATGA ATTTTTGTTC CCGTGAGAAC TTCTGGCTCT TGATCAACTC CACGGATCCC
GCAGGACAGC TCCAGTGGCT GGTGGGGGAG CTTCAGGCTG CTGAGGATCG AGGAGACAAA
GTGCATATAA TTGGCCACAT TCCCCCAGGG CACTGTCTGA AGAGCTGGAG CTGGAATTAT
TACCGAATTG TAGCCAGGTA TGAGAACACC CTGGCTGCTC AGTTCTTTGG CCACACTCAT
GTGGATGAAT TTGAGGTCTT CTATGATGAA GAGACTCTGA GCCGGCCGCT GGCTGTAGCC
TTCCTGGCAC CCAGTGCAAC TACCTACATC GGCCTTAATC CTGGTTACCG TGTGTACCAA
ATAGATGGAA ACTACTCCGG GAGCTCTCAC GTGGTCCTGG ACCATGAGAC CTACATCCTG
AATCTGACCC AGGCAAACAT ACCGGGAGCC ATACCGCACT GGCAGCTTCT CTACAGGGCT
CGAGAAACCT ATGGGCTGCC CAACACACTG CCTACCGCCT GGCACAACCT GGTATATCGC
ATGCGGGGCG ACATGCAACT TTTCCAGACC TTCTGGTTTC TCTACCATAA GGGCCACCCA
CCCTCGGAGC CCTGTGGCAC GCCCTGCCGT CTGGCTACTC TTTGTGCCCA GCTCTCTGCC
CGTGCTGACA GCCCTGCTCT GTGCCGCCAC CTGATGCCAG ATGGGAGCCT CCCAGAGGCC
CAGAGCCTGT GGCCAAGGCC ACTGTTTTGC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001007594.2
CDS186..2078
Misc Feature(1)84..86(+)
Misc Feature(2)450..668(+)
Misc Feature(3)789..1676(+)
Misc Feature(4)804..1565(+)
Misc Feature(5)804..1565(+)
Misc Feature(6)954..1745(+)
Exon (1)1..503
Gene:SMPD1
Gene Synonym:
Exon (2)504..1273
Gene:SMPD1
Gene Synonym:
Exon (3)1274..1445
Gene:SMPD1
Gene Synonym:
Exon (4)1446..1522
Gene:SMPD1
Gene Synonym:
Exon (5)1523..1668
Gene:SMPD1
Gene Synonym:
Exon (6)1669..2469
Gene:SMPD1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001007593
Organism Homo sapiens (human)
Definition Homo sapiens sphingomyelin phosphodiesterase 1, acid lysosomal (SMPD1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001007593

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
ATCAACCTCG GGCTGAAGGA ACCCAATGTG GCTCGCGTGG GCTCCGTGGC CATCAAGCTG
TGCAATCTGC TGAAGATAGC ACCACCTGCC GTGTGCCAAT CCATTGTCCA CCTCTTTGAG
GATGACATGG TGGAGGTGTG GAGACGCTCA GTGCTGAGCC CATCTGAGGC CTGTGGCCTG
CTCCTGGGCT CCACCTGTGG GCACTGGGAC ATTTTCTCAT CTTGGAACAT CTCTTTGCCT
ACTGTGCCGA AGCCGCCCCC CAAACCCCCT AGCCCCCCAG CCCCAGGTGC CCCTGTCAGC
CGCATCCTCT TCCTCACTGA CCTGCACTGG GATCATGACT ACCTGGAGGG CACGGACCCT
GACTGTGCAG ACCCACTGTG CTGCCGCCGG GGTTCTGGCC TGCCGCCCGC ATCCCGGCCA
GGTGCCGGAT ACTGGGGCGA ATACAGCAAG TGTGACCTGC CCCTGAGGAC CCTGGAGAGC
CTGTTGAGTG GGCTGGGCCC AGCCGGCCCT TTTGATATGG TGTACTGGAC AGGAGACATC
CCCGCACATG ATGTCTGGCA CCAGACTCGT CAGGACCAAC TGCGGGCCCT GACCACCGTC
ACAGCACTTG TGAGGAAGTT CCTGGGGCCA GTGCCAGTGT ACCCTGCTGT GGGTAACCAT
GAAAGCACAC CTGTCAATAG CTTCCCTCCC CCCTTCATTG AGGGCAACCA CTCCTCCCGC
TGGCTCTATG AAGCGATGGC CAAGGCTTGG GAGCCCTGGC TGCCTGCCGA AGCCCTGCGC
ACCCTCAGAA TTGGGGGGTT CTATGCTCTT TCCCCATACC CCGGTCTCCG CCTCATCTCT
CTCAATATGA ATTTTTGTTC CCGTGAGAAC TTCTGGCTCT TGATCAACTC CACGGATCCC
GCAGGACAGC TCCAGTGGCT GGTGGGGGAG CTTCAGGCTG CTGAGGATCG AGGAGACAAA
GTGCATATAA TTGGCCACAT TCCCCCAGGG CACTGTCTGA AGAGCTGGAG CTGGAATTAT
TACCGAATTG TAGCCAGGTA TGAGAACACC CTGGCTGCTC AGTTCTTTGG CCACACTCAT
GTGGATGAAT TTGAGGTCTT CTATGATGAA GAGACTCTGA GCCGGCCGCT GGCTGTAGCC
TTCCTGGCAC CCAGTGCAAC TACCTACATC GGCCTTAATC CTGGTTACCG TGTGTACCAA
ATAGATGGAA ACTACTCCGG GAGCTCTCAC GTGGTCCTGG ACCATGAGAC CTACATCCTG
AATCTGACCC AGGCAAACAT ACCGGGAGCC ATACCGCACT GGCAGCTTCT CTACAGGGCT
CGAGAAACCT ATGGGCTGCC CAACACACTG CCTACCGCCT GGCACAACCT GGTATATCGC
ATGCGGGGCG ACATGCAACT TTTCCAGACC TTCTGGTTTC TCTACCATAA GGGCCACCCA
CCCTCGGAGC CCTGTGGCAC GCCCTGCCGT CTGGCTACTC TTTGTGCCCA GCTCTCTGCC
CGTGCTGACA GCCCTGCTCT GTGCCGCCAC CTGATGCCAG ATGGGAGCCT CCCAGAGGCC
CAGAGCCTGT GGCCAAGGCC ACTGTTTTGC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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