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SMPD1 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol SMPD1
Entrez Gene ID 6609
Full Name sphingomyelin phosphodiesterase 1
Synonyms ASM,ASMASE,NPD
General protein information
Preferred Names

sphingomyelin phosphodiesterase 1

Names

sphingomyelin phosphodiesterase
acid sphingomyelinase
sphingomyelin phosphodiesterase 1, acid lysosomal

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

11

11p15.4

Disorder MIM:

607608

Disorder Html: Niemann-Pick disease, type A, 257200 (3); Niemann-Pick disease, type

mRNA and Protein(s)

mRNA Protein Name
XM_011520304.1 XP_011518606.1 sphingomyelin phosphodiesterase isoform X2
NM_001318088.1 NP_001305017.1 sphingomyelin phosphodiesterase isoform 4
NM_000543.4 NP_000534.3 sphingomyelin phosphodiesterase isoform 1 precursor
NM_001318087.1 NP_001305016.1 sphingomyelin phosphodiesterase isoform 3 precursor
XM_011520303.1 XP_011518605.1 sphingomyelin phosphodiesterase isoform X1
NM_001007593.2 NP_001007594.2 sphingomyelin phosphodiesterase isoform 2 precursor



Homo sapiens (human) SMPD1 NP_000534.3
Bos taurus (cattle) SMPD1 NP_001068655.1
Rattus norvegicus (Norway rat) Smpd1 NP_001006998.1
Danio rerio (zebrafish) smpd1 XP_683907.1
Xenopus tropicalis (tropical clawed frog) LOC100487433 XP_002943546.1
Canis lupus familiaris (dog) SMPD1 XP_542452.1
Mus musculus (house mouse) Smpd1 NP_035551.1
Neurospora crassa NCU06697 XP_961012.1
Drosophila melanogaster (fruit fly) CG3376 NP_611904.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP011940 XP_320591.3
Caenorhabditis elegans (roundworm) asm-2 NP_509894.2
Eremothecium gossypii AGOS_ACL091C NP_983313.1
Macaca mulatta (rhesus monkey) SMPD1 XP_001110212.1
Pan troglodytes (chimpanzee) SMPD1 XP_508253.2
Gallus gallus (chicken) SMPD1 XP_003640663.2


Related articles in PubMed

Acid sphingomyelinase as target of Lycium Chinense: promising new action for cell health.
Ceccarini MR, Codini M, Cataldi S, Vannini S, Lazzarini A, Floridi A, Moretti M, Villarini M, Fioretti B, Beccari T, Albi E
Lipids in health and disease15(1)183(2016 Oct)

Structural and functional analysis of the ASM p.Ala359Asp mutant that causes acid sphingomyelinase deficiency.
Acuña M, Castro-Fernández V, Latorre M, Castro J, Schuchman EH, Guixé V, González M, Zanlungo S
Biochemical and biophysical research communications479(3)496-501(2016 Oct)

Acid Sphingomyelinase (ASM) is a Negative Regulator of Regulatory T Cell (Treg) Development.
Zhou Y, Salker MS, Walker B, Münzer P, Borst O, Gawaz M, Gulbins E, Singh Y, Lang F
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology39(3)985-95(2016)

Isolation of cDNA clones encoding human acid sphingomyelinase: occurrence of alternatively processed transcripts.
Quintern LE, Schuchman EH, Levran O, Suchi M, Ferlinz K, Reinke H, Sandhoff K, Desnick RJ
The EMBO journal8(9)2469-73(1989 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Airway cells with lower SMPD1 activity increases neutrophil chemotaxis towards them.
Title: Decreasing SMPD1 activity in BEAS-2B bronchial airway epithelial cells results in increased NRF2 activity, cytokine synthesis and neutrophil recruitment.

ASM and ceramides, together with STX6 and cholesterol, constitute a new regulatory mechanism for the exit of Met from the Golgi during its biosynthetic route.
Title: Acid sphingomyelinase is required for cell surface presentation of Met receptor tyrosine kinase in cancer cells.

The results identify acid sphingomyelinase as a novel target of Lycium Chinense berries to decrease saturated/unsaturated fatty acid sphingomyelin ratio, known to be useful for cell health. Consistent with these data, the berries regulate specifically gene expression to protect cells from apoptosis.
Title: Acid sphingomyelinase as target of Lycium Chinense: promising new action for cell health.

the p.Ala359Asp mutation causes structural alterations in the hydrophobic environment where ASM is located, decreasing its enzymatic activity
Title: Structural and functional analysis of the ASM p.Ala359Asp mutant that causes acid sphingomyelinase deficiency.

ASM is a negative regulator of regulatory T Cell development.
Title: Acid Sphingomyelinase (ASM) is a Negative Regulator of Regulatory T Cell (Treg) Development.

The following SMPD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMPD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu18710
NM_000543.4
Latest version!
Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu60647 XM_011520303.1
Latest version!
Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu60648 XM_011520304.1
Latest version!
Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu101203 NM_001318088.1
Latest version!
Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $139.30
$199.00
OHu16724 NM_001007593.2
Latest version!
Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $307.30
$439.00
OHu44952 NM_001318087.1
Latest version!
Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18710
Clone ID Related Accession (Same CDS sequence) NM_000543.4
Accession Version NM_000543.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1896bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-09
Organism Homo sapiens(Human)
Product sphingomyelin phosphodiesterase isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI599208.1, AB209775.1, M59916.1 and AA746054.1. This sequence is a reference standard in the RefSeqGene project. On Jul 17, 2010 this sequence version replaced NM_000543.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB209775.1, X59960.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
ATCAACCTCG GGCTGAAGAA GGAACCCAAT GTGGCTCGCG TGGGCTCCGT GGCCATCAAG
CTGTGCAATC TGCTGAAGAT AGCACCACCT GCCGTGTGCC AATCCATTGT CCACCTCTTT
GAGGATGACA TGGTGGAGGT GTGGAGACGC TCAGTGCTGA GCCCATCTGA GGCCTGTGGC
CTGCTCCTGG GCTCCACCTG TGGGCACTGG GACATTTTCT CATCTTGGAA CATCTCTTTG
CCTACTGTGC CGAAGCCGCC CCCCAAACCC CCTAGCCCCC CAGCCCCAGG TGCCCCTGTC
AGCCGCATCC TCTTCCTCAC TGACCTGCAC TGGGATCATG ACTACCTGGA GGGCACGGAC
CCTGACTGTG CAGACCCACT GTGCTGCCGC CGGGGTTCTG GCCTGCCGCC CGCATCCCGG
CCAGGTGCCG GATACTGGGG CGAATACAGC AAGTGTGACC TGCCCCTGAG GACCCTGGAG
AGCCTGTTGA GTGGGCTGGG CCCAGCCGGC CCTTTTGATA TGGTGTACTG GACAGGAGAC
ATCCCCGCAC ATGATGTCTG GCACCAGACT CGTCAGGACC AACTGCGGGC CCTGACCACC
GTCACAGCAC TTGTGAGGAA GTTCCTGGGG CCAGTGCCAG TGTACCCTGC TGTGGGTAAC
CATGAAAGCA CACCTGTCAA TAGCTTCCCT CCCCCCTTCA TTGAGGGCAA CCACTCCTCC
CGCTGGCTCT ATGAAGCGAT GGCCAAGGCT TGGGAGCCCT GGCTGCCTGC CGAAGCCCTG
CGCACCCTCA GAATTGGGGG GTTCTATGCT CTTTCCCCAT ACCCCGGTCT CCGCCTCATC
TCTCTCAATA TGAATTTTTG TTCCCGTGAG AACTTCTGGC TCTTGATCAA CTCCACGGAT
CCCGCAGGAC AGCTCCAGTG GCTGGTGGGG GAGCTTCAGG CTGCTGAGGA TCGAGGAGAC
AAAGTGCATA TAATTGGCCA CATTCCCCCA GGGCACTGTC TGAAGAGCTG GAGCTGGAAT
TATTACCGAA TTGTAGCCAG GTATGAGAAC ACCCTGGCTG CTCAGTTCTT TGGCCACACT
CATGTGGATG AATTTGAGGT CTTCTATGAT GAAGAGACTC TGAGCCGGCC GCTGGCTGTA
GCCTTCCTGG CACCCAGTGC AACTACCTAC ATCGGCCTTA ATCCTGGTTA CCGTGTGTAC
CAAATAGATG GAAACTACTC CGGGAGCTCT CACGTGGTCC TGGACCATGA GACCTACATC
CTGAATCTGA CCCAGGCAAA CATACCGGGA GCCATACCGC ACTGGCAGCT TCTCTACAGG
GCTCGAGAAA CCTATGGGCT GCCCAACACA CTGCCTACCG CCTGGCACAA CCTGGTATAT
CGCATGCGGG GCGACATGCA ACTTTTCCAG ACCTTCTGGT TTCTCTACCA TAAGGGCCAC
CCACCCTCGG AGCCCTGTGG CACGCCCTGC CGTCTGGCTA CTCTTTGTGC CCAGCTCTCT
GCCCGTGCTG ACAGCCCTGC TCTGTGCCGC CACCTGATGC CAGATGGGAG CCTCCCAGAG
GCCCAGAGCC TGTGGCCAAG GCCACTGTTT TGCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000534.3
CDS186..2081
Misc Feature(1)84..86
Misc Feature(2)447..449
Misc Feature(3)450..671
Misc Feature(4)531..533
Misc Feature(5)order(549..551,582..584)
Misc Feature(6)792..1679
Misc Feature(7)order(807..809,813..815,1023..1025,1143..1148,1464..1466,1560..1562,1566..1568)
Misc Feature(8)order(807..809,813..815,1023..1025,1143..1145,1464..1466,1560..1562,1566..1568)
Misc Feature(9)order(852..854,867..869)
Misc Feature(10)order(870..872,939..941)
Misc Feature(11)951..1574
Misc Feature(12)1194..1196
Misc Feature(13)order(1344..1346,1482..1484)
Misc Feature(14)order(1941..1943,1953..1955)
Misc Feature(15)order(1971..1973,2010..2012)
Exon (1)1..503
Exon (2)504..1276
Exon (3)1277..1448
Exon (4)1449..1525
Exon (5)1526..1671
Exon (6)1672..2472
Translation

Target ORF information:

RefSeq Version NM_000543.4
Organism Homo sapiens(Human)
Definition Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000543.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
ATCAACCTCG GGCTGAAGAA GGAACCCAAT GTGGCTCGCG TGGGCTCCGT GGCCATCAAG
CTGTGCAATC TGCTGAAGAT AGCACCACCT GCCGTGTGCC AATCCATTGT CCACCTCTTT
GAGGATGACA TGGTGGAGGT GTGGAGACGC TCAGTGCTGA GCCCATCTGA GGCCTGTGGC
CTGCTCCTGG GCTCCACCTG TGGGCACTGG GACATTTTCT CATCTTGGAA CATCTCTTTG
CCTACTGTGC CGAAGCCGCC CCCCAAACCC CCTAGCCCCC CAGCCCCAGG TGCCCCTGTC
AGCCGCATCC TCTTCCTCAC TGACCTGCAC TGGGATCATG ACTACCTGGA GGGCACGGAC
CCTGACTGTG CAGACCCACT GTGCTGCCGC CGGGGTTCTG GCCTGCCGCC CGCATCCCGG
CCAGGTGCCG GATACTGGGG CGAATACAGC AAGTGTGACC TGCCCCTGAG GACCCTGGAG
AGCCTGTTGA GTGGGCTGGG CCCAGCCGGC CCTTTTGATA TGGTGTACTG GACAGGAGAC
ATCCCCGCAC ATGATGTCTG GCACCAGACT CGTCAGGACC AACTGCGGGC CCTGACCACC
GTCACAGCAC TTGTGAGGAA GTTCCTGGGG CCAGTGCCAG TGTACCCTGC TGTGGGTAAC
CATGAAAGCA CACCTGTCAA TAGCTTCCCT CCCCCCTTCA TTGAGGGCAA CCACTCCTCC
CGCTGGCTCT ATGAAGCGAT GGCCAAGGCT TGGGAGCCCT GGCTGCCTGC CGAAGCCCTG
CGCACCCTCA GAATTGGGGG GTTCTATGCT CTTTCCCCAT ACCCCGGTCT CCGCCTCATC
TCTCTCAATA TGAATTTTTG TTCCCGTGAG AACTTCTGGC TCTTGATCAA CTCCACGGAT
CCCGCAGGAC AGCTCCAGTG GCTGGTGGGG GAGCTTCAGG CTGCTGAGGA TCGAGGAGAC
AAAGTGCATA TAATTGGCCA CATTCCCCCA GGGCACTGTC TGAAGAGCTG GAGCTGGAAT
TATTACCGAA TTGTAGCCAG GTATGAGAAC ACCCTGGCTG CTCAGTTCTT TGGCCACACT
CATGTGGATG AATTTGAGGT CTTCTATGAT GAAGAGACTC TGAGCCGGCC GCTGGCTGTA
GCCTTCCTGG CACCCAGTGC AACTACCTAC ATCGGCCTTA ATCCTGGTTA CCGTGTGTAC
CAAATAGATG GAAACTACTC CGGGAGCTCT CACGTGGTCC TGGACCATGA GACCTACATC
CTGAATCTGA CCCAGGCAAA CATACCGGGA GCCATACCGC ACTGGCAGCT TCTCTACAGG
GCTCGAGAAA CCTATGGGCT GCCCAACACA CTGCCTACCG CCTGGCACAA CCTGGTATAT
CGCATGCGGG GCGACATGCA ACTTTTCCAG ACCTTCTGGT TTCTCTACCA TAAGGGCCAC
CCACCCTCGG AGCCCTGTGG CACGCCCTGC CGTCTGGCTA CTCTTTGTGC CCAGCTCTCT
GCCCGTGCTG ACAGCCCTGC TCTGTGCCGC CACCTGATGC CAGATGGGAG CCTCCCAGAG
GCCCAGAGCC TGTGGCCAAG GCCACTGTTT TGCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60647
Clone ID Related Accession (Same CDS sequence) XM_011520303.1
Accession Version XM_011520303.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1764bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product sphingomyelin phosphodiesterase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
ATCAACCTCG GGCTGAAGAA GGAACCCAAT GTGGCTCGCG TGGGCTCCGT GGCCATCAAG
CTGTGCAATC TGCTGAAGAT AGCACCACCT GCCGTGTGCC AATCCATTGT CCACCTCTTT
GAGGATGACA TGGTGGAGGT GTGGAGACGC TCAGTGCTGA GCCCATCTGA GGCCTGTGGC
CTGCTCCTGG GCTCCACCTG TGGGCACTGG GACATTTTCT CATCTTGGAA CATCTCTTTG
CCTACTGTGC CGAAGCCGCC CCCCAAACCC CCTAGCCCCC CAGCCCCAGG TGCCCCTGTC
AGCCGCATCC TCTTCCTCAC TGACCTGCAC TGGGATCATG ACTACCTGGA GGGCACGGAC
CCTGACTGTG CAGACCCACT GTGCTGCCGC CGGGGTTCTG GCCTGCCGCC CGCATCCCGG
CCAGGTGCCG GATACTGGGG CGAATACAGC AAGTGTGACC TGCCCCTGAG GACCCTGGAG
AGCCTGTTGA GTGGGCTGGG CCCAGCCGGC CCTTTTGATA TGGTGTACTG GACAGGAGAC
ATCCCCGCAC ATGATGTCTG GCACCAGACT CGTCAGGACC AACTGCGGGC CCTGACCACC
GTCACAGCAC TTGTGAGGAA GTTCCTGGGG CCAGTGCCAG TGTACCCTGC TGTGGGTAAC
CATGAAAGCA CACCTGTCAA TAGCTTCCCT CCCCCCTTCA TTGAGGGCAA CCACTCCTCC
CGCTGGCTCT ATGAAGCGAT GGCCAAGGCT TGGGAGCCCT GGCTGCCTGC CGAAGCCCTG
CGCACCCTCA GGTACTTATC GTCCGTGGAA ACCCAGGAAG GGAAAAGAAA GGTGCATATA
ATTGGCCACA TTCCCCCAGG GCACTGTCTG AAGAGCTGGA GCTGGAATTA TTACCGAATT
GTAGCCAGGT ATGAGAACAC CCTGGCTGCT CAGTTCTTTG GCCACACTCA TGTGGATGAA
TTTGAGGTCT TCTATGATGA AGAGACTCTG AGCCGGCCGC TGGCTGTAGC CTTCCTGGCA
CCCAGTGCAA CTACCTACAT CGGCCTTAAT CCTGGTTACC GTGTGTACCA AATAGATGGA
AACTACTCCG GGAGCTCTCA CGTGGTCCTG GACCATGAGA CCTACATCCT GAATCTGACC
CAGGCAAACA TACCGGGAGC CATACCGCAC TGGCAGCTTC TCTACAGGGC TCGAGAAACC
TATGGGCTGC CCAACACACT GCCTACCGCC TGGCACAACC TGGTATATCG CATGCGGGGC
GACATGCAAC TTTTCCAGAC CTTCTGGTTT CTCTACCATA AGGGCCACCC ACCCTCGGAG
CCCTGTGGCA CGCCCTGCCG TCTGGCTACT CTTTGTGCCC AGCTCTCTGC CCGTGCTGAC
AGCCCTGCTC TGTGCCGCCA CCTGATGCCA GATGGGAGCC TCCCAGAGGC CCAGAGCCTG
TGGCCAAGGC CACTGTTTTG CTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011518605.1
CDS299..2062
Misc Feature(1)563..784
Misc Feature(2)905..1660
Misc Feature(3)order(920..922,926..928,1136..1138,1256..1261,1445..1447,1541..1543,1547..1549)
Misc Feature(4)order(920..922,926..928,1136..1138,1256..1258,1445..1447,1541..1543,1547..1549)
Misc Feature(5)1064..1555
Translation

Target ORF information:

RefSeq Version XM_011520303.1
Organism Homo sapiens(Human)
Definition Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011520303.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
ATCAACCTCG GGCTGAAGAA GGAACCCAAT GTGGCTCGCG TGGGCTCCGT GGCCATCAAG
CTGTGCAATC TGCTGAAGAT AGCACCACCT GCCGTGTGCC AATCCATTGT CCACCTCTTT
GAGGATGACA TGGTGGAGGT GTGGAGACGC TCAGTGCTGA GCCCATCTGA GGCCTGTGGC
CTGCTCCTGG GCTCCACCTG TGGGCACTGG GACATTTTCT CATCTTGGAA CATCTCTTTG
CCTACTGTGC CGAAGCCGCC CCCCAAACCC CCTAGCCCCC CAGCCCCAGG TGCCCCTGTC
AGCCGCATCC TCTTCCTCAC TGACCTGCAC TGGGATCATG ACTACCTGGA GGGCACGGAC
CCTGACTGTG CAGACCCACT GTGCTGCCGC CGGGGTTCTG GCCTGCCGCC CGCATCCCGG
CCAGGTGCCG GATACTGGGG CGAATACAGC AAGTGTGACC TGCCCCTGAG GACCCTGGAG
AGCCTGTTGA GTGGGCTGGG CCCAGCCGGC CCTTTTGATA TGGTGTACTG GACAGGAGAC
ATCCCCGCAC ATGATGTCTG GCACCAGACT CGTCAGGACC AACTGCGGGC CCTGACCACC
GTCACAGCAC TTGTGAGGAA GTTCCTGGGG CCAGTGCCAG TGTACCCTGC TGTGGGTAAC
CATGAAAGCA CACCTGTCAA TAGCTTCCCT CCCCCCTTCA TTGAGGGCAA CCACTCCTCC
CGCTGGCTCT ATGAAGCGAT GGCCAAGGCT TGGGAGCCCT GGCTGCCTGC CGAAGCCCTG
CGCACCCTCA GGTACTTATC GTCCGTGGAA ACCCAGGAAG GGAAAAGAAA GGTGCATATA
ATTGGCCACA TTCCCCCAGG GCACTGTCTG AAGAGCTGGA GCTGGAATTA TTACCGAATT
GTAGCCAGGT ATGAGAACAC CCTGGCTGCT CAGTTCTTTG GCCACACTCA TGTGGATGAA
TTTGAGGTCT TCTATGATGA AGAGACTCTG AGCCGGCCGC TGGCTGTAGC CTTCCTGGCA
CCCAGTGCAA CTACCTACAT CGGCCTTAAT CCTGGTTACC GTGTGTACCA AATAGATGGA
AACTACTCCG GGAGCTCTCA CGTGGTCCTG GACCATGAGA CCTACATCCT GAATCTGACC
CAGGCAAACA TACCGGGAGC CATACCGCAC TGGCAGCTTC TCTACAGGGC TCGAGAAACC
TATGGGCTGC CCAACACACT GCCTACCGCC TGGCACAACC TGGTATATCG CATGCGGGGC
GACATGCAAC TTTTCCAGAC CTTCTGGTTT CTCTACCATA AGGGCCACCC ACCCTCGGAG
CCCTGTGGCA CGCCCTGCCG TCTGGCTACT CTTTGTGCCC AGCTCTCTGC CCGTGCTGAC
AGCCCTGCTC TGTGCCGCCA CCTGATGCCA GATGGGAGCC TCCCAGAGGC CCAGAGCCTG
TGGCCAAGGC CACTGTTTTG CTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60648
Clone ID Related Accession (Same CDS sequence) XM_011520304.1
Accession Version XM_011520304.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1395bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product sphingomyelin phosphodiesterase isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
ATCAACCTCG GGCTGAAGAA GGAACCCAAT GTGGCTCGCG TGGGCTCCGT GGCCATCAAG
CTGTGCAATC TGCTGAAGAT AGCACCACCT GCCGTGTGCC AATCCATTGT CCACCTCTTT
GAGGATGACA TGGTGGAGGT GTGGAGACGC TCAGTGCTGA GCCCATCTGA GGCCTGTGGC
CTGCTCCTGG GCTCCACCTG TGGGCACTGG GACATTTTCT CATCTTGGAA CATCTCTTTG
CCTACTGTGC CGAAGCCGCC CCCCAAACCC CCTAGCCCCC CAGCCCCAGG TGCCCCTGTC
AGCCGCATCC TCTTCCTCAC TGACCTGCAC TGGGATCATG ACTACCTGGA GGGCACGGAC
CCTGACTGTG CAGACCCACT GTGCTGCCGC CGGGGTTCTG GCCTGCCGCC CGCATCCCGG
CCAGGTGCCG GATACTGGGG CGAATACAGC AAGTGTGACC TGCCCCTGAG GACCCTGGAG
AGCCTGTTGA GTGGGCTGGG CCCAGCCGGC CCTTTTGATA TGGTGTACTG GACAGGAGAC
ATCCCCGCAC ATGATGTCTG GCACCAGACT CGTCAGGACC AACTGCGGGC CCTGACCACC
GTCACAGCAC TTGTGAGGAA GTTCCTGGGG CCAGTGCCAG TGTACCCTGC TGTGGGTAAC
CATGAAAGCA CACCTGTCAA TAGCTTCCCT CCCCCCTTCA TTGAGGGCAA CCACTCCTCC
CGCTGGCTCT ATGAAGCGAT GGCCAAGGCT TGGGAGCCCT GGCTGCCTGC CGAAGCCCTG
CGCACCCTCA GGTACTTATC GTCCGTGGAA ACCCAGGAAG GGAAAAGAAA GGTGCATATA
ATTGGCCACA TTCCCCCAGG GCACTGTCTG AAGAGCTGGA GCTGGAATTA TTACCGAATT
GTAGCCAGGT ATGAGAACAC CCTGGCTGCT CAGTTCTTTG GCCACACTCA TGTGGATGAA
TTTGAGGTCT TCTATGATGA AGAGACTCTG AGCCGGCCGC TGGCTGTAGC CTTCCTGGCA
CCCAGTGCAA CTACCTACAT CGGCCTTAAT CCTGTCAGCC CCACATCCTT GCAGGTTACC
GTGTGTACCA AATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011518606.1
CDS299..1693
Misc Feature(1)563..784
Misc Feature(2)905..1651
Misc Feature(3)order(920..922,926..928,1136..1138,1256..1261,1445..1447,1541..1543,1547..1549)
Misc Feature(4)order(920..922,926..928,1136..1138,1256..1258,1445..1447,1541..1543,1547..1549)
Misc Feature(5)1064..1555
Translation

Target ORF information:

RefSeq Version XM_011520304.1
Organism Homo sapiens(Human)
Definition Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011520304.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
ATCAACCTCG GGCTGAAGAA GGAACCCAAT GTGGCTCGCG TGGGCTCCGT GGCCATCAAG
CTGTGCAATC TGCTGAAGAT AGCACCACCT GCCGTGTGCC AATCCATTGT CCACCTCTTT
GAGGATGACA TGGTGGAGGT GTGGAGACGC TCAGTGCTGA GCCCATCTGA GGCCTGTGGC
CTGCTCCTGG GCTCCACCTG TGGGCACTGG GACATTTTCT CATCTTGGAA CATCTCTTTG
CCTACTGTGC CGAAGCCGCC CCCCAAACCC CCTAGCCCCC CAGCCCCAGG TGCCCCTGTC
AGCCGCATCC TCTTCCTCAC TGACCTGCAC TGGGATCATG ACTACCTGGA GGGCACGGAC
CCTGACTGTG CAGACCCACT GTGCTGCCGC CGGGGTTCTG GCCTGCCGCC CGCATCCCGG
CCAGGTGCCG GATACTGGGG CGAATACAGC AAGTGTGACC TGCCCCTGAG GACCCTGGAG
AGCCTGTTGA GTGGGCTGGG CCCAGCCGGC CCTTTTGATA TGGTGTACTG GACAGGAGAC
ATCCCCGCAC ATGATGTCTG GCACCAGACT CGTCAGGACC AACTGCGGGC CCTGACCACC
GTCACAGCAC TTGTGAGGAA GTTCCTGGGG CCAGTGCCAG TGTACCCTGC TGTGGGTAAC
CATGAAAGCA CACCTGTCAA TAGCTTCCCT CCCCCCTTCA TTGAGGGCAA CCACTCCTCC
CGCTGGCTCT ATGAAGCGAT GGCCAAGGCT TGGGAGCCCT GGCTGCCTGC CGAAGCCCTG
CGCACCCTCA GGTACTTATC GTCCGTGGAA ACCCAGGAAG GGAAAAGAAA GGTGCATATA
ATTGGCCACA TTCCCCCAGG GCACTGTCTG AAGAGCTGGA GCTGGAATTA TTACCGAATT
GTAGCCAGGT ATGAGAACAC CCTGGCTGCT CAGTTCTTTG GCCACACTCA TGTGGATGAA
TTTGAGGTCT TCTATGATGA AGAGACTCTG AGCCGGCCGC TGGCTGTAGC CTTCCTGGCA
CCCAGTGCAA CTACCTACAT CGGCCTTAAT CCTGTCAGCC CCACATCCTT GCAGGTTACC
GTGTGTACCA AATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu101203
Clone ID Related Accession (Same CDS sequence) NM_001318088.1
Accession Version NM_001318088.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 975bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-09
Organism Homo sapiens(Human)
Product sphingomyelin phosphodiesterase isoform 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI599208.1, HQ132748.1, M59916.1, AB209775.1 and BM977655.1. On Dec 23, 2015 this sequence version replaced XR_930886.1. Transcript Variant: This variant (4) differs in the 5' UTR and uses an alternate translation start site compared to variant 1. The encoded isoform (4) has a shorter and distinct N-terminus, and lacks a predicted signal peptide compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HQ132748.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGAAAGCAC ACCTGTCAAT AGCTTCCCTC CCCCCTTCAT TGAGGGCAAC CACTCCTCCC 
GCTGGCTCTA TGAAGCGATG GCCAAGGCTT GGGAGCCCTG GCTGCCTGCC GAAGCCCTGC
GCACCCTCAG GTACTTATCG TCCGTGGAAA CCCAGGAAGG GAAAAGAAAG AATTGGGGGG
TTCTATGCTC TTTCCCCATA CCCCGGTCTC CGCCTCATCT CTCTCAATAT GAATTTTTGT
TCCCGTGAGA ACTTCTGGCT CTTGATCAAC TCCACGGATC CCGCAGGACA GCTCCAGTGG
CTGGTGGGGG AGCTTCAGGC TGCTGAGGAT CGAGGAGACA AAGTGCATAT AATTGGCCAC
ATTCCCCCAG GGCACTGTCT GAAGAGCTGG AGCTGGAATT ATTACCGAAT TGTAGCCAGG
TATGAGAACA CCCTGGCTGC TCAGTTCTTT GGCCACACTC ATGTGGATGA ATTTGAGGTC
TTCTATGATG AAGAGACTCT GAGCCGGCCG CTGGCTGTAG CCTTCCTGGC ACCCAGTGCA
ACTACCTACA TCGGCCTTAA TCCTGGTTAC CGTGTGTACC AAATAGATGG AAACTACTCC
GGGAGCTCTC ACGTGGTCCT GGACCATGAG ACCTACATCC TGAATCTGAC CCAGGCAAAC
ATACCGGGAG CCATACCGCA CTGGCAGCTT CTCTACAGGG CTCGAGAAAC CTATGGGCTG
CCCAACACAC TGCCTACCGC CTGGCACAAC CTGGTATATC GCATGCGGGG CGACATGCAA
CTTTTCCAGA CCTTCTGGTT TCTCTACCAT AAGGGCCACC CACCCTCGGA GCCCTGTGGC
ACGCCCTGCC GTCTGGCTAC TCTTTGTGCC CAGCTCTCTG CCCGTGCTGA CAGCCCTGCT
CTGTGCCGCC ACCTGATGCC AGATGGGAGC CTCCCAGAGG CCCAGAGCCT GTGGCCAAGG
CCACTGTTTT GCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001305017.1
CDS1147..2121
Misc Feature(1)1099..1101
Misc Feature(2)<1285..1719
Misc Feature(3)<1429..1788
Exon (1)1..503
Exon (2)504..1316
Exon (3)1317..1488
Exon (4)1489..1565
Exon (5)1566..1711
Exon (6)1712..2512
Translation

Target ORF information:

RefSeq Version NM_001318088.1
Organism Homo sapiens(Human)
Definition Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001318088.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGAAAGCAC ACCTGTCAAT AGCTTCCCTC CCCCCTTCAT TGAGGGCAAC CACTCCTCCC 
GCTGGCTCTA TGAAGCGATG GCCAAGGCTT GGGAGCCCTG GCTGCCTGCC GAAGCCCTGC
GCACCCTCAG GTACTTATCG TCCGTGGAAA CCCAGGAAGG GAAAAGAAAG AATTGGGGGG
TTCTATGCTC TTTCCCCATA CCCCGGTCTC CGCCTCATCT CTCTCAATAT GAATTTTTGT
TCCCGTGAGA ACTTCTGGCT CTTGATCAAC TCCACGGATC CCGCAGGACA GCTCCAGTGG
CTGGTGGGGG AGCTTCAGGC TGCTGAGGAT CGAGGAGACA AAGTGCATAT AATTGGCCAC
ATTCCCCCAG GGCACTGTCT GAAGAGCTGG AGCTGGAATT ATTACCGAAT TGTAGCCAGG
TATGAGAACA CCCTGGCTGC TCAGTTCTTT GGCCACACTC ATGTGGATGA ATTTGAGGTC
TTCTATGATG AAGAGACTCT GAGCCGGCCG CTGGCTGTAG CCTTCCTGGC ACCCAGTGCA
ACTACCTACA TCGGCCTTAA TCCTGGTTAC CGTGTGTACC AAATAGATGG AAACTACTCC
GGGAGCTCTC ACGTGGTCCT GGACCATGAG ACCTACATCC TGAATCTGAC CCAGGCAAAC
ATACCGGGAG CCATACCGCA CTGGCAGCTT CTCTACAGGG CTCGAGAAAC CTATGGGCTG
CCCAACACAC TGCCTACCGC CTGGCACAAC CTGGTATATC GCATGCGGGG CGACATGCAA
CTTTTCCAGA CCTTCTGGTT TCTCTACCAT AAGGGCCACC CACCCTCGGA GCCCTGTGGC
ACGCCCTGCC GTCTGGCTAC TCTTTGTGCC CAGCTCTCTG CCCGTGCTGA CAGCCCTGCT
CTGTGCCGCC ACCTGATGCC AGATGGGAGC CTCCCAGAGG CCCAGAGCCT GTGGCCAAGG
CCACTGTTTT GCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16724
Clone ID Related Accession (Same CDS sequence) NM_001007593.2
Accession Version NM_001007593.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1893bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-09
Organism Homo sapiens(Human)
Product sphingomyelin phosphodiesterase isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI599208.1, AB209775.1, M59916.1 and AA746054.1. On Jul 17, 2010 this sequence version replaced NM_001007593.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The encoded protein (isoform 2) is one amino acid shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292388.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142363 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
ATCAACCTCG GGCTGAAGGA ACCCAATGTG GCTCGCGTGG GCTCCGTGGC CATCAAGCTG
TGCAATCTGC TGAAGATAGC ACCACCTGCC GTGTGCCAAT CCATTGTCCA CCTCTTTGAG
GATGACATGG TGGAGGTGTG GAGACGCTCA GTGCTGAGCC CATCTGAGGC CTGTGGCCTG
CTCCTGGGCT CCACCTGTGG GCACTGGGAC ATTTTCTCAT CTTGGAACAT CTCTTTGCCT
ACTGTGCCGA AGCCGCCCCC CAAACCCCCT AGCCCCCCAG CCCCAGGTGC CCCTGTCAGC
CGCATCCTCT TCCTCACTGA CCTGCACTGG GATCATGACT ACCTGGAGGG CACGGACCCT
GACTGTGCAG ACCCACTGTG CTGCCGCCGG GGTTCTGGCC TGCCGCCCGC ATCCCGGCCA
GGTGCCGGAT ACTGGGGCGA ATACAGCAAG TGTGACCTGC CCCTGAGGAC CCTGGAGAGC
CTGTTGAGTG GGCTGGGCCC AGCCGGCCCT TTTGATATGG TGTACTGGAC AGGAGACATC
CCCGCACATG ATGTCTGGCA CCAGACTCGT CAGGACCAAC TGCGGGCCCT GACCACCGTC
ACAGCACTTG TGAGGAAGTT CCTGGGGCCA GTGCCAGTGT ACCCTGCTGT GGGTAACCAT
GAAAGCACAC CTGTCAATAG CTTCCCTCCC CCCTTCATTG AGGGCAACCA CTCCTCCCGC
TGGCTCTATG AAGCGATGGC CAAGGCTTGG GAGCCCTGGC TGCCTGCCGA AGCCCTGCGC
ACCCTCAGAA TTGGGGGGTT CTATGCTCTT TCCCCATACC CCGGTCTCCG CCTCATCTCT
CTCAATATGA ATTTTTGTTC CCGTGAGAAC TTCTGGCTCT TGATCAACTC CACGGATCCC
GCAGGACAGC TCCAGTGGCT GGTGGGGGAG CTTCAGGCTG CTGAGGATCG AGGAGACAAA
GTGCATATAA TTGGCCACAT TCCCCCAGGG CACTGTCTGA AGAGCTGGAG CTGGAATTAT
TACCGAATTG TAGCCAGGTA TGAGAACACC CTGGCTGCTC AGTTCTTTGG CCACACTCAT
GTGGATGAAT TTGAGGTCTT CTATGATGAA GAGACTCTGA GCCGGCCGCT GGCTGTAGCC
TTCCTGGCAC CCAGTGCAAC TACCTACATC GGCCTTAATC CTGGTTACCG TGTGTACCAA
ATAGATGGAA ACTACTCCGG GAGCTCTCAC GTGGTCCTGG ACCATGAGAC CTACATCCTG
AATCTGACCC AGGCAAACAT ACCGGGAGCC ATACCGCACT GGCAGCTTCT CTACAGGGCT
CGAGAAACCT ATGGGCTGCC CAACACACTG CCTACCGCCT GGCACAACCT GGTATATCGC
ATGCGGGGCG ACATGCAACT TTTCCAGACC TTCTGGTTTC TCTACCATAA GGGCCACCCA
CCCTCGGAGC CCTGTGGCAC GCCCTGCCGT CTGGCTACTC TTTGTGCCCA GCTCTCTGCC
CGTGCTGACA GCCCTGCTCT GTGCCGCCAC CTGATGCCAG ATGGGAGCCT CCCAGAGGCC
CAGAGCCTGT GGCCAAGGCC ACTGTTTTGC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001007594.2
CDS186..2078
Misc Feature(1)84..86
Misc Feature(2)450..668
Misc Feature(3)789..1676
Misc Feature(4)order(804..806,810..812,1020..1022,1140..1145,1461..1463,1557..1559,1563..1565)
Misc Feature(5)order(804..806,810..812,1020..1022,1140..1142,1461..1463,1557..1559,1563..1565)
Misc Feature(6)948..1571
Exon (1)1..503
Exon (2)504..1273
Exon (3)1274..1445
Exon (4)1446..1522
Exon (5)1523..1668
Exon (6)1669..2469
Translation

Target ORF information:

RefSeq Version NM_001007593.2
Organism Homo sapiens(Human)
Definition Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001007593.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
ATCAACCTCG GGCTGAAGGA ACCCAATGTG GCTCGCGTGG GCTCCGTGGC CATCAAGCTG
TGCAATCTGC TGAAGATAGC ACCACCTGCC GTGTGCCAAT CCATTGTCCA CCTCTTTGAG
GATGACATGG TGGAGGTGTG GAGACGCTCA GTGCTGAGCC CATCTGAGGC CTGTGGCCTG
CTCCTGGGCT CCACCTGTGG GCACTGGGAC ATTTTCTCAT CTTGGAACAT CTCTTTGCCT
ACTGTGCCGA AGCCGCCCCC CAAACCCCCT AGCCCCCCAG CCCCAGGTGC CCCTGTCAGC
CGCATCCTCT TCCTCACTGA CCTGCACTGG GATCATGACT ACCTGGAGGG CACGGACCCT
GACTGTGCAG ACCCACTGTG CTGCCGCCGG GGTTCTGGCC TGCCGCCCGC ATCCCGGCCA
GGTGCCGGAT ACTGGGGCGA ATACAGCAAG TGTGACCTGC CCCTGAGGAC CCTGGAGAGC
CTGTTGAGTG GGCTGGGCCC AGCCGGCCCT TTTGATATGG TGTACTGGAC AGGAGACATC
CCCGCACATG ATGTCTGGCA CCAGACTCGT CAGGACCAAC TGCGGGCCCT GACCACCGTC
ACAGCACTTG TGAGGAAGTT CCTGGGGCCA GTGCCAGTGT ACCCTGCTGT GGGTAACCAT
GAAAGCACAC CTGTCAATAG CTTCCCTCCC CCCTTCATTG AGGGCAACCA CTCCTCCCGC
TGGCTCTATG AAGCGATGGC CAAGGCTTGG GAGCCCTGGC TGCCTGCCGA AGCCCTGCGC
ACCCTCAGAA TTGGGGGGTT CTATGCTCTT TCCCCATACC CCGGTCTCCG CCTCATCTCT
CTCAATATGA ATTTTTGTTC CCGTGAGAAC TTCTGGCTCT TGATCAACTC CACGGATCCC
GCAGGACAGC TCCAGTGGCT GGTGGGGGAG CTTCAGGCTG CTGAGGATCG AGGAGACAAA
GTGCATATAA TTGGCCACAT TCCCCCAGGG CACTGTCTGA AGAGCTGGAG CTGGAATTAT
TACCGAATTG TAGCCAGGTA TGAGAACACC CTGGCTGCTC AGTTCTTTGG CCACACTCAT
GTGGATGAAT TTGAGGTCTT CTATGATGAA GAGACTCTGA GCCGGCCGCT GGCTGTAGCC
TTCCTGGCAC CCAGTGCAAC TACCTACATC GGCCTTAATC CTGGTTACCG TGTGTACCAA
ATAGATGGAA ACTACTCCGG GAGCTCTCAC GTGGTCCTGG ACCATGAGAC CTACATCCTG
AATCTGACCC AGGCAAACAT ACCGGGAGCC ATACCGCACT GGCAGCTTCT CTACAGGGCT
CGAGAAACCT ATGGGCTGCC CAACACACTG CCTACCGCCT GGCACAACCT GGTATATCGC
ATGCGGGGCG ACATGCAACT TTTCCAGACC TTCTGGTTTC TCTACCATAA GGGCCACCCA
CCCTCGGAGC CCTGTGGCAC GCCCTGCCGT CTGGCTACTC TTTGTGCCCA GCTCTCTGCC
CGTGCTGACA GCCCTGCTCT GTGCCGCCAC CTGATGCCAG ATGGGAGCCT CCCAGAGGCC
CAGAGCCTGT GGCCAAGGCC ACTGTTTTGC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu44952
Clone ID Related Accession (Same CDS sequence) NM_001318087.1
Accession Version NM_001318087.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1527bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-09
Organism Homo sapiens(Human)
Product sphingomyelin phosphodiesterase isoform 3 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI599208.1, HQ132747.1, M59916.1, BC041164.1 and AA746054.1. On Dec 23, 2015 this sequence version replaced XM_005253075.3. Transcript Variant: This variant (3) uses an alternate splice site in its 3' exon resulting in a frameshift and an early stop codon compared to variant 1. The encoded isoform (3) has a shorter and distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HQ132747.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
ATCAACCTCG GGCTGAAGAA GGAACCCAAT GTGGCTCGCG TGGGCTCCGT GGCCATCAAG
CTGTGCAATC TGCTGAAGAT AGCACCACCT GCCGTGTGCC AATCCATTGT CCACCTCTTT
GAGGATGACA TGGTGGAGGT GTGGAGACGC TCAGTGCTGA GCCCATCTGA GGCCTGTGGC
CTGCTCCTGG GCTCCACCTG TGGGCACTGG GACATTTTCT CATCTTGGAA CATCTCTTTG
CCTACTGTGC CGAAGCCGCC CCCCAAACCC CCTAGCCCCC CAGCCCCAGG TGCCCCTGTC
AGCCGCATCC TCTTCCTCAC TGACCTGCAC TGGGATCATG ACTACCTGGA GGGCACGGAC
CCTGACTGTG CAGACCCACT GTGCTGCCGC CGGGGTTCTG GCCTGCCGCC CGCATCCCGG
CCAGGTGCCG GATACTGGGG CGAATACAGC AAGTGTGACC TGCCCCTGAG GACCCTGGAG
AGCCTGTTGA GTGGGCTGGG CCCAGCCGGC CCTTTTGATA TGGTGTACTG GACAGGAGAC
ATCCCCGCAC ATGATGTCTG GCACCAGACT CGTCAGGACC AACTGCGGGC CCTGACCACC
GTCACAGCAC TTGTGAGGAA GTTCCTGGGG CCAGTGCCAG TGTACCCTGC TGTGGGTAAC
CATGAAAGCA CACCTGTCAA TAGCTTCCCT CCCCCCTTCA TTGAGGGCAA CCACTCCTCC
CGCTGGCTCT ATGAAGCGAT GGCCAAGGCT TGGGAGCCCT GGCTGCCTGC CGAAGCCCTG
CGCACCCTCA GAATTGGGGG GTTCTATGCT CTTTCCCCAT ACCCCGGTCT CCGCCTCATC
TCTCTCAATA TGAATTTTTG TTCCCGTGAG AACTTCTGGC TCTTGATCAA CTCCACGGAT
CCCGCAGGAC AGCTCCAGTG GCTGGTGGGG GAGCTTCAGG CTGCTGAGGA TCGAGGAGAC
AAAGTGCATA TAATTGGCCA CATTCCCCCA GGGCACTGTC TGAAGAGCTG GAGCTGGAAT
TATTACCGAA TTGTAGCCAG GTATGAGAAC ACCCTGGCTG CTCAGTTCTT TGGCCACACT
CATGTGGATG AATTTGAGGT CTTCTATGAT GAAGAGACTC TGAGCCGGCC GCTGGCTGTA
GCCTTCCTGG CACCCAGTGC AACTACCTAC ATCGGCCTTA ATCCTGTCAG CCCCACATCC
TTGCAGGTTA CCGTGTGTAC CAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001305016.1
CDS186..1712
Misc Feature(1)84..86
Misc Feature(2)450..671
Misc Feature(3)792..1670
Misc Feature(4)order(807..809,813..815,1023..1025,1143..1148,1464..1466,1560..1562,1566..1568)
Misc Feature(5)order(807..809,813..815,1023..1025,1143..1145,1464..1466,1560..1562,1566..1568)
Misc Feature(6)951..1574
Exon (1)1..503
Exon (2)504..1276
Exon (3)1277..1448
Exon (4)1449..1525
Exon (5)1526..1671
Exon (6)1672..2492
Translation

Target ORF information:

RefSeq Version NM_001318087.1
Organism Homo sapiens(Human)
Definition Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001318087.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGCCCCGCT ACGGAGCGTC ACTCCGCCAG AGCTGCCCCA GGTCCGGCCG GGAGCAGGGA 
CAAGACGGGA CCGCCGGAGC CCCCGGACTC CTTTGGATGG GCCTGGTGCT GGCGCTGGCG
CTGGCGCTGG CGCTGGCGCT GGCTCTGTCT GACTCTCGGG TTCTCTGGGC TCCGGCAGAG
GCTCACCCTC TTTCTCCCCA AGGCCATCCT GCCAGGTTAC ATCGCATAGT GCCCCGGCTC
CGAGATGTCT TTGGGTGGGG GAACCTCACC TGCCCAATCT GCAAAGGTCT ATTCACCGCC
ATCAACCTCG GGCTGAAGAA GGAACCCAAT GTGGCTCGCG TGGGCTCCGT GGCCATCAAG
CTGTGCAATC TGCTGAAGAT AGCACCACCT GCCGTGTGCC AATCCATTGT CCACCTCTTT
GAGGATGACA TGGTGGAGGT GTGGAGACGC TCAGTGCTGA GCCCATCTGA GGCCTGTGGC
CTGCTCCTGG GCTCCACCTG TGGGCACTGG GACATTTTCT CATCTTGGAA CATCTCTTTG
CCTACTGTGC CGAAGCCGCC CCCCAAACCC CCTAGCCCCC CAGCCCCAGG TGCCCCTGTC
AGCCGCATCC TCTTCCTCAC TGACCTGCAC TGGGATCATG ACTACCTGGA GGGCACGGAC
CCTGACTGTG CAGACCCACT GTGCTGCCGC CGGGGTTCTG GCCTGCCGCC CGCATCCCGG
CCAGGTGCCG GATACTGGGG CGAATACAGC AAGTGTGACC TGCCCCTGAG GACCCTGGAG
AGCCTGTTGA GTGGGCTGGG CCCAGCCGGC CCTTTTGATA TGGTGTACTG GACAGGAGAC
ATCCCCGCAC ATGATGTCTG GCACCAGACT CGTCAGGACC AACTGCGGGC CCTGACCACC
GTCACAGCAC TTGTGAGGAA GTTCCTGGGG CCAGTGCCAG TGTACCCTGC TGTGGGTAAC
CATGAAAGCA CACCTGTCAA TAGCTTCCCT CCCCCCTTCA TTGAGGGCAA CCACTCCTCC
CGCTGGCTCT ATGAAGCGAT GGCCAAGGCT TGGGAGCCCT GGCTGCCTGC CGAAGCCCTG
CGCACCCTCA GAATTGGGGG GTTCTATGCT CTTTCCCCAT ACCCCGGTCT CCGCCTCATC
TCTCTCAATA TGAATTTTTG TTCCCGTGAG AACTTCTGGC TCTTGATCAA CTCCACGGAT
CCCGCAGGAC AGCTCCAGTG GCTGGTGGGG GAGCTTCAGG CTGCTGAGGA TCGAGGAGAC
AAAGTGCATA TAATTGGCCA CATTCCCCCA GGGCACTGTC TGAAGAGCTG GAGCTGGAAT
TATTACCGAA TTGTAGCCAG GTATGAGAAC ACCCTGGCTG CTCAGTTCTT TGGCCACACT
CATGTGGATG AATTTGAGGT CTTCTATGAT GAAGAGACTC TGAGCCGGCC GCTGGCTGTA
GCCTTCCTGG CACCCAGTGC AACTACCTAC ATCGGCCTTA ATCCTGTCAG CCCCACATCC
TTGCAGGTTA CCGTGTGTAC CAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Acid sphingomyelinase as target of Lycium Chinense: promising new action for cell health.
Lipids in health and disease15(1)183(2016 Oct)
Ceccarini MR,Codini M,Cataldi S,Vannini S,Lazzarini A,Floridi A,Moretti M,Villarini M,Fioretti B,Beccari T,Albi E


book

Structural and functional analysis of the ASM p.Ala359Asp mutant that causes acid sphingomyelinase deficiency.
Biochemical and biophysical research communications479(3)496-501(2016 Oct)
Acuña M,Castro-Fernández V,Latorre M,Castro J,Schuchman EH,Guixé V,González M,Zanlungo S


book

Acid Sphingomyelinase (ASM) is a Negative Regulator of Regulatory T Cell (Treg) Development.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology39(3)985-95(2016)
Zhou Y,Salker MS,Walker B,Münzer P,Borst O,Gawaz M,Gulbins E,Singh Y,Lang F


book

Isolation of cDNA clones encoding human acid sphingomyelinase: occurrence of alternatively processed transcripts.
The EMBO journal8(9)2469-73(1989 Sep)
Quintern LE,Schuchman EH,Levran O,Suchi M,Ferlinz K,Reinke H,Sandhoff K,Desnick RJ


 
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