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SPINK1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol SPINK1
Entrez Gene ID 6690
Full Name serine peptidase inhibitor, Kazal type 1
Synonyms PCTT, PSTI, Spink3, TATI, TCP
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

5

5q32

Summary The protein encoded by this gene is a trypsin inhibitor, which is secreted from pancreatic acinar cells into pancreatic juice. It is thought to function in the prevention of trypsin-catalyzed premature activation of zymogens within the pancreas and the pancreatic duct. Mutations in this gene are associated with hereditary pancreatitis and tropical calcific pancreatitis. [provided by RefSeq, Oct 2008]. lac of sum
Disorder MIM:

167790

Disorder Html: Pancreatitis, hereditary, 167800 (3); {Fibrocalculous pancreatic

mRNA and Protein(s)

mRNA Protein Name
NM_003122 NP_003113 pancreatic secretory trypsin inhibitor precursor



Homo sapiens (human) SPINK1 NP_003113.2
Pan troglodytes (chimpanzee) SPINK1 XP_001160275.1
Macaca mulatta (Rhesus monkey) SPINK1 XP_001102888.1
Canis lupus familiaris (dog) SPINK1 XP_850557.1
Bos taurus (cattle) SPINK1 NP_001020519.1
Mus musculus (house mouse) Spink3 NP_033284.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SPINK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPINK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu28321
NM_003122 Homo sapiens serine peptidase inhibitor, Kazal type 1 (SPINK1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu28321
Clone ID Related Accession (Same CDS sequence) NM_003122
Accession Version NM_003122.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 240bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pancreatic secretory trypsin inhibitor precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB159576.1, BC025790.1 and BU074107.1. This sequence is a reference standard in the RefSeqGene project. On or before Jun 20, 2014 this sequence version replaced gi:530380662, gi:195234783. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CB159576.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
ATGAAGGTAA CAGGCATCTT TCTTCTCAGT GCCTTGGCCC TGTTGAGTCT ATCTGGTAAC 
ACTGGAGCTG ACTCCCTGGG AAGAGAGGCC AAATGTTACA ATGAACTTAA TGGATGCACC
AAGATATATG ACCCTGTCTG TGGGACTGAT GGAAATACTT ATCCCAATGA ATGCGTGTTA
TGTTTTGAAA ATCGGAAACG CCAGACTTCT ATCCTCATTC AAAAATCTGG GCCTTGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003113.2
CDS290..529
Misc Feature(1)242..244(+)
Misc Feature(2)392..526(+)
Misc Feature(3)410..415(+)
Misc Feature(4)410..415(+)
Misc Feature(5)410..412(+)
Exon (1)1..98
Gene:SPINK1
Gene Synonym:
Exon (2)99..344
Gene:SPINK1
Gene Synonym:
Exon (3)345..376
Gene:SPINK1
Gene Synonym:
Exon (4)377..483
Gene:SPINK1
Gene Synonym:
Exon (5)484..610
Gene:SPINK1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_003122
Organism Homo sapiens (human)
Definition Homo sapiens serine peptidase inhibitor, Kazal type 1 (SPINK1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_003122

ORF Insert Sequence:

1
61
121
181
ATGAAGGTAA CAGGCATCTT TCTTCTCAGT GCCTTGGCCC TGTTGAGTCT ATCTGGTAAC 
ACTGGAGCTG ACTCCCTGGG AAGAGAGGCC AAATGTTACA ATGAACTTAA TGGATGCACC
AAGATATATG ACCCTGTCTG TGGGACTGAT GGAAATACTT ATCCCAATGA ATGCGTGTTA
TGTTTTGAAA ATCGGAAACG CCAGACTTCT ATCCTCATTC AAAAATCTGG GCCTTGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Assessment of the tumor-associated trypsin inhibitor (TATI) marker in patients with carcinoma of the uterine body 17 years after treatment
Eur. J. Gynaecol. Oncol. 35 (1), 39-43 (2014)
Kozakiewicz,B., Chadzynska,M. and Dmoch-Gajzlerska,E.


book

The relationship between serum tumor-associated trypsin inhibitor levels and clinicopathological parameters in patients with gastric cancer
Eur Rev Med Pharmacol Sci 17 (21), 2923-2928 (2013)
Kemik O, Kemik A, Sumer A, Almali N, Gurluler E, Gures N, Purisa S, Adas G, Dogan Y and Tuzun S.


book

Childhood-onset hereditary pancreatitis with mutations in the CT gene and SPINK1 gene
Pediatr Int 55 (5), 646-649 (2013)
Awano H, Lee T, Yagi M, Masamune A, Kume K, Takeshima Y and Iijima K.


book

Cyst fluid SPINK1 may help to differentiate benign and potentially malignant cystic pancreatic lesions
Pancreatology 13 (5), 530-533 (2013)
Raty S, Sand J, Laukkarinen J, Vasama K, Bassi C, Salvia R and Nordback I.


book

Upstream open reading frames cause widespread reduction of protein expression and are polymorphic among humans
Proc. Natl. Acad. Sci. U.S.A. 106 (18), 7507-7512 (2009)
Calvo SE, Pagliarini DJ and Mootha VK.


book

Mutations in the gene encoding the serine protease inhibitor, Kazal type 1 are associated with chronic pancreatitis
Nat. Genet. 25 (2), 213-216 (2000)
Witt H, Luck W, Hennies HC, Classen M, Kage A, Lass U, Landt O and Becker M.


book

Pancreatitis Overview
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
LaRusch,J., Solomon,S. and Whitcomb,D.C.


book

Three-dimensional structure of a recombinant variant of human pancreatic secretory trypsin inhibitor (Kazal type)
J. Mol. Biol. 225 (4), 1095-1103 (1992)
Hecht HJ, Szardenings M, Collins J and Schomburg D.


book

Interactions of pancreatic secretory trypsin inhibitor in small intestinal juice: its hydrolysis and protection by intraluminal factors
Clin. Chim. Acta 195 (1-2), 27-39 (1990)
Freeman TC, Davies R and Calam J.


book

The primary structure of the human pancreatic secretory trypsin inhibitor. Amino acid sequence of the reduced S-aminoethylated protein
Arch. Biochem. Biophys. 179 (1), 189-199 (1977)
Bartelt,D.C., Shapanka,R. and Greene,L.J.


 
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