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SPINK1 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol SPINK1
Entrez Gene ID 6690
Full Name serine peptidase inhibitor, Kazal type 1
Synonyms PCTT,PSTI,Spink3,TATI,TCP
General protein information
Preferred Names

serine peptidase inhibitor, Kazal type 1

Names

serine protease inhibitor Kazal-type 1
pancreatic secretory trypsin inhibitor
serine protease inhibitor, Kazal type 1
tumor-associated trypsin inhibitor

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

5

5q32

Summary The protein encoded by this gene is a trypsin inhibitor, which is secreted from pancreatic acinar cells into pancreatic juice. It is thought to function in the prevention of trypsin-catalyzed premature activation of zymogens within the pancreas and the pancreatic duct. Mutations in this gene are associated with hereditary pancreatitis and tropical calcific pancreatitis. [provided by RefSeq, Oct 2008].
Disorder MIM:

167790

Disorder Html: Pancreatitis, hereditary, 167800 (3); {Fibrocalculous pancreatic

mRNA and Protein(s)

mRNA Protein Name
NM_003122.4 NP_003113.2 serine protease inhibitor Kazal-type 1 precursor



Pan troglodytes (chimpanzee) SPINK1 XP_001160275.1
Macaca mulatta (rhesus monkey) SPINK1 XP_001102888.1
Bos taurus (cattle) SPINK1 NP_001020519.1
Mus musculus (house mouse) Spink3 NP_033284.1
Homo sapiens (human) SPINK1 NP_003113.2
Canis lupus familiaris (dog) SPINK1 XP_850557.1


Related articles in PubMed

SPINK1 Promoter Variants Are Associated with Prostate Cancer Predisposing Alterations in Benign Prostatic Hyperplasia Patients.
Winchester D, Ricks-Santi L, Mason T, Abbas M, Copeland RL Jr, Beyene D, Jingwi EY, Dunston GM, Kanaan YM
Anticancer research35(7)3811-9(2015 Jul)

Functional significance of SPINK1 promoter variants in chronic pancreatitis.
Derikx MH, Geisz A, Kereszturi É, Sahin-Tóth M
American journal of physiology. Gastrointestinal and liver physiology308(9)G779-84(2015 May)

Gene expression analysis for evaluation of potential biomarkers in hepatocellular carcinoma.
Hass HG, Hass H, Jobst J, Scheurlen M, Vogel U, Nehls O
Anticancer research35(4)2021-8(2015 Apr)

Claudin-5 is associated with elevated TATI and CA125 levels in mucinous ovarian borderline tumors.
Nissi R, Talvensaari-Mattila A, Kuvaja P, Pääkkö P, Soini Y, Santala M
Anticancer research35(2)973-6(2015 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Absolute exclusivity between SPINK1 overexpression and homozygous PTEN deletion in localized PCA.
Title: SPINK1 Overexpression in Localized Prostate Cancer: a Rare Event Inversely Associated with ERG Expression and Exclusive of Homozygous PTEN Deletion.

Sixteen patients exhibited heterozygous pathogenic variants of SPINK1, including c.194+2T>C (n=12), p.N34S (n=3), and a novel pathogenic splicing variation c.194+1G>A.
Title: PRSS1, SPINK1, CFTR, and CTRC Pathogenic Variants in Korean Patients With Idiopathic Pancreatitis.

We describe a case of malignant pancreatic cancer diagnosed in a young patient with chronic pancreatitis who is a SPINK 1 heterozygote gene mutation carrier
Title: Metastatic pancreatic adenocarcinoma associated with chronic calcific pancreatitis and a heterozygous SPINK1 N34S mutation.

It is the first time to simultaneously detect SPINK1 and ERG expression in initially diagnosed bone metastatic prostate cancer. The over-expression of SPINK1 was not only related to poor PSA response, but also significantly associated with the occurrence of castration-resistant prostate cancer, especially in those with much more aggressive phenotype
Title: The expression profile and prognostic value of SPINK1 in initially diagnosed bone metastatic prostate cancer.

Approx 25% of discontinuously involved prostate biopsy cores showed tumor foci with discordant ERG/SPINK1 status, consistent with multiclonal disease.
Title: Clonal evaluation of prostate cancer foci in biopsies with discontinuous tumor involvement by dual ERG/SPINK1 immunohistochemistry.

The following SPINK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPINK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu28321
NM_003122.4
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Homo sapiens serine peptidase inhibitor, Kazal type 1 (SPINK1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu28321
Clone ID Related Accession (Same CDS sequence) NM_003122.4
Accession Version NM_003122.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 240bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-11-22
Organism Homo sapiens(Human)
Product serine protease inhibitor Kazal-type 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB159576.1, BC025790.1 and BU074107.1. This sequence is a reference standard in the RefSeqGene project. On or before Jun 20, 2014 this sequence version replaced XM_005268501.1, NM_003122.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CB159576.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
ATGAAGGTAA CAGGCATCTT TCTTCTCAGT GCCTTGGCCC TGTTGAGTCT ATCTGGTAAC 
ACTGGAGCTG ACTCCCTGGG AAGAGAGGCC AAATGTTACA ATGAACTTAA TGGATGCACC
AAGATATATG ACCCTGTCTG TGGGACTGAT GGAAATACTT ATCCCAATGA ATGCGTGTTA
TGTTTTGAAA ATCGGAAACG CCAGACTTCT ATCCTCATTC AAAAATCTGG GCCTTGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003113.2
CDS290..529
Misc Feature(1)242..244
Misc Feature(2)392..526
Misc Feature(3)410..415
Misc Feature(4)410..415
Misc Feature(5)410..412
Misc Feature(6)416..421
Exon (1)1..98
Exon (2)99..344
Exon (3)345..376
Exon (4)377..483
Exon (5)484..610
Translation

Target ORF information:

RefSeq Version NM_003122.4
Organism Homo sapiens(Human)
Definition Homo sapiens serine peptidase inhibitor, Kazal type 1 (SPINK1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_003122.4

ORF Insert Sequence:

1
61
121
181
ATGAAGGTAA CAGGCATCTT TCTTCTCAGT GCCTTGGCCC TGTTGAGTCT ATCTGGTAAC 
ACTGGAGCTG ACTCCCTGGG AAGAGAGGCC AAATGTTACA ATGAACTTAA TGGATGCACC
AAGATATATG ACCCTGTCTG TGGGACTGAT GGAAATACTT ATCCCAATGA ATGCGTGTTA
TGTTTTGAAA ATCGGAAACG CCAGACTTCT ATCCTCATTC AAAAATCTGG GCCTTGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

SPINK1 Promoter Variants Are Associated with Prostate Cancer Predisposing Alterations in Benign Prostatic Hyperplasia Patients.
Anticancer research35(7)3811-9(2015 Jul)
Winchester D,Ricks-Santi L,Mason T,Abbas M,Copeland RL Jr,Beyene D,Jingwi EY,Dunston GM,Kanaan YM


book

Functional significance of SPINK1 promoter variants in chronic pancreatitis.
American journal of physiology. Gastrointestinal and liver physiology308(9)G779-84(2015 May)
Derikx MH,Geisz A,Kereszturi É,Sahin-Tóth M


book

Gene expression analysis for evaluation of potential biomarkers in hepatocellular carcinoma.
Anticancer research35(4)2021-8(2015 Apr)
Hass HG,Hass H,Jobst J,Scheurlen M,Vogel U,Nehls O


book

Claudin-5 is associated with elevated TATI and CA125 levels in mucinous ovarian borderline tumors.
Anticancer research35(2)973-6(2015 Feb)
Nissi R,Talvensaari-Mattila A,Kuvaja P,Pääkkö P,Soini Y,Santala M


 
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