SRP54 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Gene Symbol	SRP54
Entrez Gene ID	6729
Full Name	signal recognition particle 54
General protein information	Preferred Names signal recognition particle 54 Names signal recognition particle 54 kDa protein signal recognition particle 54kD signal recognition particle 54kDa
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q13.2
Disorder MIM:	604857

mRNA and Protein(s)

mRNA	Protein	Name
XM_011537106.1	XP_011535408.1	signal recognition particle 54 kDa protein isoform X1
XM_005268024.3	XP_005268081.1	signal recognition particle 54 kDa protein isoform X2
NM_003136.3	NP_003127.1	signal recognition particle 54 kDa protein isoform 1
NM_003136.4	NP_003127.1	signal recognition particle 54 kDa protein isoform 1
XM_005268024.2	XP_005268081.1	signal recognition particle 54 kDa protein isoform X2
XM_017021615.2	XP_016877104.1	signal recognition particle 54 kDa protein isoform X3
XM_017021615.1	XP_016877104.1	signal recognition particle 54 kDa protein isoform X3
NM_001146282.1	NP_001139754.1	signal recognition particle 54 kDa protein isoform 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Srp54a	NP_036029.2
Danio rerio (zebrafish)	srp54	NP_957282.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004610	XP_315212.2
Caenorhabditis elegans (roundworm)	F21D5.7	NP_501507.1
Saccharomyces cerevisiae (baker's yeast)	SRP54	NP_015413.1
Kluyveromyces lactis	KLLA0D09020g	XP_453464.1
Homo sapiens (human)	SRP54	NP_003127.1
Rattus norvegicus (Norway rat)	Srp54a	NP_446323.1
Magnaporthe oryzae (rice blast fungus)	MGG_05977	XP_003711822.1
Arabidopsis thaliana (thale cress)	AT1G48900	NP_564535.1
Canis lupus familiaris (dog)	SRP54	NP_001003272.1
Bos taurus (cattle)	SRP54	NP_001033290.1
Gallus gallus (chicken)	SRP54	XP_421238.1
Schizosaccharomyces pombe (fission yeast)	srp54	NP_588209.1
Neurospora crassa	NCU09696	XP_960643.1
Oryza sativa (rice)	Os01g0772800	NP_001044395.1
Pan troglodytes (chimpanzee)	SRP54	XP_001136048.1
	SRP54	XP_001082531.1
Mus musculus (house mouse)	Srp54b	NP_001093579.1
Drosophila melanogaster (fruit fly)	Srp54k	NP_523931.1
Eremothecium gossypii	AGOS_AAR041W	NP_982582.2
Xenopus tropicalis (tropical clawed frog)	srp54	NP_988977.1

Gene Ontology
Bibliography

Related articles in PubMed

Mutations in signal recognition particle SRP54 cause syndromic neutropenia with Shwachman-Diamond-like features.
Carapito R, Konantz M, Paillard C, Miao Z, Pichot A, Leduc MS, Yang Y, Bergstrom KL, Mahoney DH, Shardy DL, Alsaleh G, Naegely L, Kolmer A, Paul N, Hanauer A, Rolli V, M?ller JS, Alghisi E, Sauteur L, Macquin C, Morlon A, Sancho CS, Amati-Bonneau P, Procaccio V, Mosca-Boidron AL, Marle N, Osmani N, Lefebvre O, Goetz JG, Unal S, Akarsu NA, Radosavljevic M, Chenard MP, Rialland F, Grain A, B?n? MC, Eveillard M, Vincent M, Guy J, Faivre L, Thauvin-Robinet C, Thevenon J, Myers K, Fleming MD, Shimamura A, Bottollier-Lemallaz E, Westhof E, Lengerke C, Isidor B, Bahram S
The Journal of clinical investigation127(11)4090-4103(2017 Nov)

Host factors that interact with the pestivirus N-terminal protease, Npro, are components of the ribonucleoprotein complex.
Jefferson M, Donaszi-Ivanov A, Pollen S, Dalmay T, Saalbach G, Powell PP
Journal of virology88(18)10340-53(2014 Sep)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

autosomal dominant mutations in SRP54, a key member of the cotranslation protein-targeting pathway, lead to syndromic neutropenia with a Shwachman-Diamond-like phenotype.
Title: Mutations in signal recognition particle SRP54 cause syndromic neutropenia with Shwachman-Diamond-like features.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

crystal structure of a human SRP ternary complex consisting of SRP19, the M domain of SRP54 and the S domain of 7SL RNA
Title: Induced structural changes of 7SL RNA during the assembly of human signal recognition particle.

The following SRP54 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SRP54 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26455	NM_003136.4 Latest version!	Homo sapiens signal recognition particle 54 (SRP54), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu26455	XM_011537106.1 Latest version!	Homo sapiens signal recognition particle 54 (SRP54), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu60733	XM_017021615.2 Latest version!	Homo sapiens signal recognition particle 54 (SRP54), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu26546	NM_001146282.1 Latest version!	Homo sapiens signal recognition particle 54 (SRP54), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu47874	XM_005268024.3 Latest version!	Homo sapiens signal recognition particle 54 (SRP54), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu26455	NM_003136.3	Homo sapiens signal recognition particle 54 (SRP54), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu47874	XM_005268024.2	Homo sapiens signal recognition particle 54kDa (SRP54), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu60733	XM_017021615.1	Homo sapiens signal recognition particle 54kDa (SRP54), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SRP54 ( NM_003136.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003127.1 Homo sapiens signal recognition particle 54 (SRP54), transcript variant 1, mRNA.

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CloneID	OHu26455
Clone ID Related Accession (Same CDS sequence)	XM_011537106.1 , NM_003136.3 , NM_003136.4
Accession Version	NM_003136.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-29
Organism	Homo sapiens(human)
Product	signal recognition particle 54 kDa protein isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA495064.1 and BC000652.2. On Apr 4, 2009 this sequence version replaced NM_003136.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.126494.1, SRR1803612.156525.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTTCTAG CAGACCTTGG AAGAAAAATA ACATCAGCAT TACGCTCGTT GAGCAATGCC 
ACCATTATCA ATGAAGAGGT ATTGAATGCT ATGCTAAAAG AAGTCTGTAC CGCTTTGTTG 
GAAGCAGATG TTAATATTAA ACTAGTGAAG CAACTAAGAG AAAATGTTAA GTCTGCTATT 
GATCTTGAAG AGATGGCATC TGGTCTTAAC AAAAGAAAAA TGATTCAGCA TGCTGTATTT 
AAAGAACTTG TGAAGCTTGT AGACCCTGGA GTTAAGGCAT GGACACCCAC TAAAGGAAAA 
CAAAATGTGA TTATGTTTGT TGGATTGCAA GGGAGTGGTA AAACAACAAC ATGTTCAAAG 
CTAGCATATT ATTACCAGAG GAAAGGTTGG AAGACCTGTT TAATATGTGC AGACACATTC 
AGAGCAGGGG CTTTTGACCA ACTAAAACAG AATGCTACCA AAGCAAGAAT TCCATTTTAT 
GGAAGCTATA CAGAAATGGA TCCTGTCATC ATTGCTTCTG AAGGAGTAGA GAAATTTAAA 
AATGAAAATT TTGAAATTAT TATTGTTGAT ACAAGTGGCC GCCACAAACA AGAAGACTCT 
TTGTTTGAAG AAATGCTTCA AGTTGCTAAT GCTATACAAC CTGATAACAT TGTTTATGTG 
ATGGATGCCT CCATTGGGCA GGCTTGTGAA GCCCAGGCTA AGGCTTTTAA AGATAAAGTA 
GATGTAGCCT CAGTAATAGT GACAAAACTT GATGGCCATG CAAAAGGAGG TGGTGCACTC 
AGTGCAGTCG CTGCCACAAA AAGTCCGATT ATTTTCATTG GTACAGGGGA ACATATAGAT 
GACTTTGAAC CTTTCAAAAC ACAGCCTTTT ATTAGCAAAC TTCTTGGTAT GGGCGACATT 
GAAGGACTGA TAGATAAAGT CAACGAGTTG AAGTTGGATG ACAATGAAGC ACTTATAGAG 
AAGTTGAAAC ATGGTCAGTT TACGTTGCGA GACATGTATG AGCAATTTCA AAATATCATG 
AAAATGGGCC CCTTCAGTCA GATCTTGGGG ATGATCCCTG GTTTTGGGAC AGATTTTATG 
AGCAAAGGAA ATGAACAGGA GTCAATGGCA AGGCTAAAGA AATTAATGAC AATAATGGAT 
AGTATGAATG ATCAAGAACT AGACAGTACG GATGGTGCCA AAGTTTTTAG TAAACAACCA 
GGAAGAATCC AAAGAGTAGC AAGAGGATCG GGTGTATCAA CAAGAGATGT TCAAGAACTT 
TTGACACAAT ATACCAAGTT TGCACAGATG GTAAAAAAGA TGGGAGGTAT CAAAGGACTT 
TTCAAAGGTG GCGACATGTC TAAGAATGTG AGCCAGTCAC AGATGGCAAA ATTGAACCAA 
CAAATGGCCA AAATGATGGA TCCTAGGGTT CTTCATCACA TGGGTGGTAT GGCAGGACTT 
CAGTCAATGA TGAGGCAGTT TCAACAGGGT GCTGCTGGCA ACATGAAAGG CATGATGGGA 
TTCAATAATA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003127.1
CDS	352..1866
Translation	MVLADLGRKITSALRSLSNATIINEEVLNAMLKEVCTALLEADVNIKLVKQLRENVKSAIDLEEMASGLNKRKMIQHAVFKELVKLVDPGVKAWTPTKGKQNVIMFVGLQGSGKTTTCSKLAYYYQRKGWKTCLICADTFRAGAFDQLKQNATKARIPFYGSYTEMDPVIIASEGVEKFKNENFEIIIVDTSGRHKQEDSLFEEMLQVANAIQPDNIVYVMDASIGQACEAQAKAFKDKVDVASVIVTKLDGHAKGGGALSAVAATKSPIIFIGTGEHIDDFEPFKTQPFISKLLGMGDIEGLIDKVNELKLDDNEALIEKLKHGQFTLRDMYEQFQNIMKMGPFSQILGMIPGFGTDFMSKGNEQESMARLKKLMTIMDSMNDQELDSTDGAKVFSKQPGRIQRVARGSGVSTRDVQELLTQYTKFAQMVKKMGGIKGLFKGGDMSKNVSQSQMAKLNQQMAKMMDPRVLHHMGGMAGLQSMMRQFQQGAAGNMKGMMGFNNM

Target ORF information:

RefSeq Version	NM_003136.3
Organism	Homo sapiens(human)
Definition	Homo sapiens signal recognition particle 54 (SRP54), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003136.3

ORF Insert Sequence:

ATGGTTCTAG CAGACCTTGG AAGAAAAATA ACATCAGCAT TACGCTCGTT GAGCAATGCC 
ACCATTATCA ATGAAGAGGT ATTGAATGCT ATGCTAAAAG AAGTCTGTAC CGCTTTGTTG 
GAAGCAGATG TTAATATTAA ACTAGTGAAG CAACTAAGAG AAAATGTTAA GTCTGCTATT 
GATCTTGAAG AGATGGCATC TGGTCTTAAC AAAAGAAAAA TGATTCAGCA TGCTGTATTT 
AAAGAACTTG TGAAGCTTGT AGACCCTGGA GTTAAGGCAT GGACACCCAC TAAAGGAAAA 
CAAAATGTGA TTATGTTTGT TGGATTGCAA GGGAGTGGTA AAACAACAAC ATGTTCAAAG 
CTAGCATATT ATTACCAGAG GAAAGGTTGG AAGACCTGTT TAATATGTGC AGACACATTC 
AGAGCAGGGG CTTTTGACCA ACTAAAACAG AATGCTACCA AAGCAAGAAT TCCATTTTAT 
GGAAGCTATA CAGAAATGGA TCCTGTCATC ATTGCTTCTG AAGGAGTAGA GAAATTTAAA 
AATGAAAATT TTGAAATTAT TATTGTTGAT ACAAGTGGCC GCCACAAACA AGAAGACTCT 
TTGTTTGAAG AAATGCTTCA AGTTGCTAAT GCTATACAAC CTGATAACAT TGTTTATGTG 
ATGGATGCCT CCATTGGGCA GGCTTGTGAA GCCCAGGCTA AGGCTTTTAA AGATAAAGTA 
GATGTAGCCT CAGTAATAGT GACAAAACTT GATGGCCATG CAAAAGGAGG TGGTGCACTC 
AGTGCAGTCG CTGCCACAAA AAGTCCGATT ATTTTCATTG GTACAGGGGA ACATATAGAT 
GACTTTGAAC CTTTCAAAAC ACAGCCTTTT ATTAGCAAAC TTCTTGGTAT GGGCGACATT 
GAAGGACTGA TAGATAAAGT CAACGAGTTG AAGTTGGATG ACAATGAAGC ACTTATAGAG 
AAGTTGAAAC ATGGTCAGTT TACGTTGCGA GACATGTATG AGCAATTTCA AAATATCATG 
AAAATGGGCC CCTTCAGTCA GATCTTGGGG ATGATCCCTG GTTTTGGGAC AGATTTTATG 
AGCAAAGGAA ATGAACAGGA GTCAATGGCA AGGCTAAAGA AATTAATGAC AATAATGGAT 
AGTATGAATG ATCAAGAACT AGACAGTACG GATGGTGCCA AAGTTTTTAG TAAACAACCA 
GGAAGAATCC AAAGAGTAGC AAGAGGATCG GGTGTATCAA CAAGAGATGT TCAAGAACTT 
TTGACACAAT ATACCAAGTT TGCACAGATG GTAAAAAAGA TGGGAGGTAT CAAAGGACTT 
TTCAAAGGTG GCGACATGTC TAAGAATGTG AGCCAGTCAC AGATGGCAAA ATTGAACCAA 
CAAATGGCCA AAATGATGGA TCCTAGGGTT CTTCATCACA TGGGTGGTAT GGCAGGACTT 
CAGTCAATGA TGAGGCAGTT TCAACAGGGT GCTGCTGGCA ACATGAAAGG CATGATGGGA 
TTCAATAATA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SRP54 ( NM_003136.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003127.1 Homo sapiens signal recognition particle 54 (SRP54), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26455
Clone ID Related Accession (Same CDS sequence)	XM_011537106.1 , NM_003136.3 , NM_003136.4
Accession Version	NM_003136.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-13
Organism	Homo sapiens(human)
Product	signal recognition particle 54 kDa protein isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA495064.1 and BC000652.2. On Nov 22, 2018 this sequence version replaced NM_003136.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.126494.1, SRR5189658.194175.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000216774.11/ ENSP00000216774.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTTCTAG CAGACCTTGG AAGAAAAATA ACATCAGCAT TACGCTCGTT GAGCAATGCC 
ACCATTATCA ATGAAGAGGT ATTGAATGCT ATGCTAAAAG AAGTCTGTAC CGCTTTGTTG 
GAAGCAGATG TTAATATTAA ACTAGTGAAG CAACTAAGAG AAAATGTTAA GTCTGCTATT 
GATCTTGAAG AGATGGCATC TGGTCTTAAC AAAAGAAAAA TGATTCAGCA TGCTGTATTT 
AAAGAACTTG TGAAGCTTGT AGACCCTGGA GTTAAGGCAT GGACACCCAC TAAAGGAAAA 
CAAAATGTGA TTATGTTTGT TGGATTGCAA GGGAGTGGTA AAACAACAAC ATGTTCAAAG 
CTAGCATATT ATTACCAGAG GAAAGGTTGG AAGACCTGTT TAATATGTGC AGACACATTC 
AGAGCAGGGG CTTTTGACCA ACTAAAACAG AATGCTACCA AAGCAAGAAT TCCATTTTAT 
GGAAGCTATA CAGAAATGGA TCCTGTCATC ATTGCTTCTG AAGGAGTAGA GAAATTTAAA 
AATGAAAATT TTGAAATTAT TATTGTTGAT ACAAGTGGCC GCCACAAACA AGAAGACTCT 
TTGTTTGAAG AAATGCTTCA AGTTGCTAAT GCTATACAAC CTGATAACAT TGTTTATGTG 
ATGGATGCCT CCATTGGGCA GGCTTGTGAA GCCCAGGCTA AGGCTTTTAA AGATAAAGTA 
GATGTAGCCT CAGTAATAGT GACAAAACTT GATGGCCATG CAAAAGGAGG TGGTGCACTC 
AGTGCAGTCG CTGCCACAAA AAGTCCGATT ATTTTCATTG GTACAGGGGA ACATATAGAT 
GACTTTGAAC CTTTCAAAAC ACAGCCTTTT ATTAGCAAAC TTCTTGGTAT GGGCGACATT 
GAAGGACTGA TAGATAAAGT CAACGAGTTG AAGTTGGATG ACAATGAAGC ACTTATAGAG 
AAGTTGAAAC ATGGTCAGTT TACGTTGCGA GACATGTATG AGCAATTTCA AAATATCATG 
AAAATGGGCC CCTTCAGTCA GATCTTGGGG ATGATCCCTG GTTTTGGGAC AGATTTTATG 
AGCAAAGGAA ATGAACAGGA GTCAATGGCA AGGCTAAAGA AATTAATGAC AATAATGGAT 
AGTATGAATG ATCAAGAACT AGACAGTACG GATGGTGCCA AAGTTTTTAG TAAACAACCA 
GGAAGAATCC AAAGAGTAGC AAGAGGATCG GGTGTATCAA CAAGAGATGT TCAAGAACTT 
TTGACACAAT ATACCAAGTT TGCACAGATG GTAAAAAAGA TGGGAGGTAT CAAAGGACTT 
TTCAAAGGTG GCGACATGTC TAAGAATGTG AGCCAGTCAC AGATGGCAAA ATTGAACCAA 
CAAATGGCCA AAATGATGGA TCCTAGGGTT CTTCATCACA TGGGTGGTAT GGCAGGACTT 
CAGTCAATGA TGAGGCAGTT TCAACAGGGT GCTGCTGGCA ACATGAAAGG CATGATGGGA 
TTCAATAATA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003127.1
CDS	258..1772
Translation	MVLADLGRKITSALRSLSNATIINEEVLNAMLKEVCTALLEADVNIKLVKQLRENVKSAIDLEEMASGLNKRKMIQHAVFKELVKLVDPGVKAWTPTKGKQNVIMFVGLQGSGKTTTCSKLAYYYQRKGWKTCLICADTFRAGAFDQLKQNATKARIPFYGSYTEMDPVIIASEGVEKFKNENFEIIIVDTSGRHKQEDSLFEEMLQVANAIQPDNIVYVMDASIGQACEAQAKAFKDKVDVASVIVTKLDGHAKGGGALSAVAATKSPIIFIGTGEHIDDFEPFKTQPFISKLLGMGDIEGLIDKVNELKLDDNEALIEKLKHGQFTLRDMYEQFQNIMKMGPFSQILGMIPGFGTDFMSKGNEQESMARLKKLMTIMDSMNDQELDSTDGAKVFSKQPGRIQRVARGSGVSTRDVQELLTQYTKFAQMVKKMGGIKGLFKGGDMSKNVSQSQMAKLNQQMAKMMDPRVLHHMGGMAGLQSMMRQFQQGAAGNMKGMMGFNNM

Target ORF information:

RefSeq Version	NM_003136.4
Organism	Homo sapiens(human)
Definition	Homo sapiens signal recognition particle 54 (SRP54), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003136.4

ORF Insert Sequence:

ATGGTTCTAG CAGACCTTGG AAGAAAAATA ACATCAGCAT TACGCTCGTT GAGCAATGCC 
ACCATTATCA ATGAAGAGGT ATTGAATGCT ATGCTAAAAG AAGTCTGTAC CGCTTTGTTG 
GAAGCAGATG TTAATATTAA ACTAGTGAAG CAACTAAGAG AAAATGTTAA GTCTGCTATT 
GATCTTGAAG AGATGGCATC TGGTCTTAAC AAAAGAAAAA TGATTCAGCA TGCTGTATTT 
AAAGAACTTG TGAAGCTTGT AGACCCTGGA GTTAAGGCAT GGACACCCAC TAAAGGAAAA 
CAAAATGTGA TTATGTTTGT TGGATTGCAA GGGAGTGGTA AAACAACAAC ATGTTCAAAG 
CTAGCATATT ATTACCAGAG GAAAGGTTGG AAGACCTGTT TAATATGTGC AGACACATTC 
AGAGCAGGGG CTTTTGACCA ACTAAAACAG AATGCTACCA AAGCAAGAAT TCCATTTTAT 
GGAAGCTATA CAGAAATGGA TCCTGTCATC ATTGCTTCTG AAGGAGTAGA GAAATTTAAA 
AATGAAAATT TTGAAATTAT TATTGTTGAT ACAAGTGGCC GCCACAAACA AGAAGACTCT 
TTGTTTGAAG AAATGCTTCA AGTTGCTAAT GCTATACAAC CTGATAACAT TGTTTATGTG 
ATGGATGCCT CCATTGGGCA GGCTTGTGAA GCCCAGGCTA AGGCTTTTAA AGATAAAGTA 
GATGTAGCCT CAGTAATAGT GACAAAACTT GATGGCCATG CAAAAGGAGG TGGTGCACTC 
AGTGCAGTCG CTGCCACAAA AAGTCCGATT ATTTTCATTG GTACAGGGGA ACATATAGAT 
GACTTTGAAC CTTTCAAAAC ACAGCCTTTT ATTAGCAAAC TTCTTGGTAT GGGCGACATT 
GAAGGACTGA TAGATAAAGT CAACGAGTTG AAGTTGGATG ACAATGAAGC ACTTATAGAG 
AAGTTGAAAC ATGGTCAGTT TACGTTGCGA GACATGTATG AGCAATTTCA AAATATCATG 
AAAATGGGCC CCTTCAGTCA GATCTTGGGG ATGATCCCTG GTTTTGGGAC AGATTTTATG 
AGCAAAGGAA ATGAACAGGA GTCAATGGCA AGGCTAAAGA AATTAATGAC AATAATGGAT 
AGTATGAATG ATCAAGAACT AGACAGTACG GATGGTGCCA AAGTTTTTAG TAAACAACCA 
GGAAGAATCC AAAGAGTAGC AAGAGGATCG GGTGTATCAA CAAGAGATGT TCAAGAACTT 
TTGACACAAT ATACCAAGTT TGCACAGATG GTAAAAAAGA TGGGAGGTAT CAAAGGACTT 
TTCAAAGGTG GCGACATGTC TAAGAATGTG AGCCAGTCAC AGATGGCAAA ATTGAACCAA 
CAAATGGCCA AAATGATGGA TCCTAGGGTT CTTCATCACA TGGGTGGTAT GGCAGGACTT 
CAGTCAATGA TGAGGCAGTT TCAACAGGGT GCTGCTGGCA ACATGAAAGG CATGATGGGA 
TTCAATAATA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SRP54 ( XM_011537106.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011535408.1 Homo sapiens signal recognition particle 54 (SRP54), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu26455
Clone ID Related Accession (Same CDS sequence)	XM_011537106.1 , NM_003136.3 , NM_003136.4
Accession Version	XM_011537106.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	signal recognition particle 54 kDa protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCTAG CAGACCTTGG AAGAAAAATA ACATCAGCAT TACGCTCGTT GAGCAATGCC 
ACCATTATCA ATGAAGAGGT ATTGAATGCT ATGCTAAAAG AAGTCTGTAC CGCTTTGTTG 
GAAGCAGATG TTAATATTAA ACTAGTGAAG CAACTAAGAG AAAATGTTAA GTCTGCTATT 
GATCTTGAAG AGATGGCATC TGGTCTTAAC AAAAGAAAAA TGATTCAGCA TGCTGTATTT 
AAAGAACTTG TGAAGCTTGT AGACCCTGGA GTTAAGGCAT GGACACCCAC TAAAGGAAAA 
CAAAATGTGA TTATGTTTGT TGGATTGCAA GGGAGTGGTA AAACAACAAC ATGTTCAAAG 
CTAGCATATT ATTACCAGAG GAAAGGTTGG AAGACCTGTT TAATATGTGC AGACACATTC 
AGAGCAGGGG CTTTTGACCA ACTAAAACAG AATGCTACCA AAGCAAGAAT TCCATTTTAT 
GGAAGCTATA CAGAAATGGA TCCTGTCATC ATTGCTTCTG AAGGAGTAGA GAAATTTAAA 
AATGAAAATT TTGAAATTAT TATTGTTGAT ACAAGTGGCC GCCACAAACA AGAAGACTCT 
TTGTTTGAAG AAATGCTTCA AGTTGCTAAT GCTATACAAC CTGATAACAT TGTTTATGTG 
ATGGATGCCT CCATTGGGCA GGCTTGTGAA GCCCAGGCTA AGGCTTTTAA AGATAAAGTA 
GATGTAGCCT CAGTAATAGT GACAAAACTT GATGGCCATG CAAAAGGAGG TGGTGCACTC 
AGTGCAGTCG CTGCCACAAA AAGTCCGATT ATTTTCATTG GTACAGGGGA ACATATAGAT 
GACTTTGAAC CTTTCAAAAC ACAGCCTTTT ATTAGCAAAC TTCTTGGTAT GGGCGACATT 
GAAGGACTGA TAGATAAAGT CAACGAGTTG AAGTTGGATG ACAATGAAGC ACTTATAGAG 
AAGTTGAAAC ATGGTCAGTT TACGTTGCGA GACATGTATG AGCAATTTCA AAATATCATG 
AAAATGGGCC CCTTCAGTCA GATCTTGGGG ATGATCCCTG GTTTTGGGAC AGATTTTATG 
AGCAAAGGAA ATGAACAGGA GTCAATGGCA AGGCTAAAGA AATTAATGAC AATAATGGAT 
AGTATGAATG ATCAAGAACT AGACAGTACG GATGGTGCCA AAGTTTTTAG TAAACAACCA 
GGAAGAATCC AAAGAGTAGC AAGAGGATCG GGTGTATCAA CAAGAGATGT TCAAGAACTT 
TTGACACAAT ATACCAAGTT TGCACAGATG GTAAAAAAGA TGGGAGGTAT CAAAGGACTT 
TTCAAAGGTG GCGACATGTC TAAGAATGTG AGCCAGTCAC AGATGGCAAA ATTGAACCAA 
CAAATGGCCA AAATGATGGA TCCTAGGGTT CTTCATCACA TGGGTGGTAT GGCAGGACTT 
CAGTCAATGA TGAGGCAGTT TCAACAGGGT GCTGCTGGCA ACATGAAAGG CATGATGGGA 
TTCAATAATA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011535408.1
CDS	233..1747
Translation	MVLADLGRKITSALRSLSNATIINEEVLNAMLKEVCTALLEADVNIKLVKQLRENVKSAIDLEEMASGLNKRKMIQHAVFKELVKLVDPGVKAWTPTKGKQNVIMFVGLQGSGKTTTCSKLAYYYQRKGWKTCLICADTFRAGAFDQLKQNATKARIPFYGSYTEMDPVIIASEGVEKFKNENFEIIIVDTSGRHKQEDSLFEEMLQVANAIQPDNIVYVMDASIGQACEAQAKAFKDKVDVASVIVTKLDGHAKGGGALSAVAATKSPIIFIGTGEHIDDFEPFKTQPFISKLLGMGDIEGLIDKVNELKLDDNEALIEKLKHGQFTLRDMYEQFQNIMKMGPFSQILGMIPGFGTDFMSKGNEQESMARLKKLMTIMDSMNDQELDSTDGAKVFSKQPGRIQRVARGSGVSTRDVQELLTQYTKFAQMVKKMGGIKGLFKGGDMSKNVSQSQMAKLNQQMAKMMDPRVLHHMGGMAGLQSMMRQFQQGAAGNMKGMMGFNNM

Target ORF information:

RefSeq Version	XM_011537106.1
Organism	Homo sapiens(human)
Definition	Homo sapiens signal recognition particle 54 (SRP54), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537106.1

ORF Insert Sequence:

ATGGTTCTAG CAGACCTTGG AAGAAAAATA ACATCAGCAT TACGCTCGTT GAGCAATGCC 
ACCATTATCA ATGAAGAGGT ATTGAATGCT ATGCTAAAAG AAGTCTGTAC CGCTTTGTTG 
GAAGCAGATG TTAATATTAA ACTAGTGAAG CAACTAAGAG AAAATGTTAA GTCTGCTATT 
GATCTTGAAG AGATGGCATC TGGTCTTAAC AAAAGAAAAA TGATTCAGCA TGCTGTATTT 
AAAGAACTTG TGAAGCTTGT AGACCCTGGA GTTAAGGCAT GGACACCCAC TAAAGGAAAA 
CAAAATGTGA TTATGTTTGT TGGATTGCAA GGGAGTGGTA AAACAACAAC ATGTTCAAAG 
CTAGCATATT ATTACCAGAG GAAAGGTTGG AAGACCTGTT TAATATGTGC AGACACATTC 
AGAGCAGGGG CTTTTGACCA ACTAAAACAG AATGCTACCA AAGCAAGAAT TCCATTTTAT 
GGAAGCTATA CAGAAATGGA TCCTGTCATC ATTGCTTCTG AAGGAGTAGA GAAATTTAAA 
AATGAAAATT TTGAAATTAT TATTGTTGAT ACAAGTGGCC GCCACAAACA AGAAGACTCT 
TTGTTTGAAG AAATGCTTCA AGTTGCTAAT GCTATACAAC CTGATAACAT TGTTTATGTG 
ATGGATGCCT CCATTGGGCA GGCTTGTGAA GCCCAGGCTA AGGCTTTTAA AGATAAAGTA 
GATGTAGCCT CAGTAATAGT GACAAAACTT GATGGCCATG CAAAAGGAGG TGGTGCACTC 
AGTGCAGTCG CTGCCACAAA AAGTCCGATT ATTTTCATTG GTACAGGGGA ACATATAGAT 
GACTTTGAAC CTTTCAAAAC ACAGCCTTTT ATTAGCAAAC TTCTTGGTAT GGGCGACATT 
GAAGGACTGA TAGATAAAGT CAACGAGTTG AAGTTGGATG ACAATGAAGC ACTTATAGAG 
AAGTTGAAAC ATGGTCAGTT TACGTTGCGA GACATGTATG AGCAATTTCA AAATATCATG 
AAAATGGGCC CCTTCAGTCA GATCTTGGGG ATGATCCCTG GTTTTGGGAC AGATTTTATG 
AGCAAAGGAA ATGAACAGGA GTCAATGGCA AGGCTAAAGA AATTAATGAC AATAATGGAT 
AGTATGAATG ATCAAGAACT AGACAGTACG GATGGTGCCA AAGTTTTTAG TAAACAACCA 
GGAAGAATCC AAAGAGTAGC AAGAGGATCG GGTGTATCAA CAAGAGATGT TCAAGAACTT 
TTGACACAAT ATACCAAGTT TGCACAGATG GTAAAAAAGA TGGGAGGTAT CAAAGGACTT 
TTCAAAGGTG GCGACATGTC TAAGAATGTG AGCCAGTCAC AGATGGCAAA ATTGAACCAA 
CAAATGGCCA AAATGATGGA TCCTAGGGTT CTTCATCACA TGGGTGGTAT GGCAGGACTT 
CAGTCAATGA TGAGGCAGTT TCAACAGGGT GCTGCTGGCA ACATGAAAGG CATGATGGGA 
TTCAATAATA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SRP54 ( XM_017021615.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877104.1 Homo sapiens signal recognition particle 54 (SRP54), transcript variant X3, mRNA.

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CloneID	OHu60733
Clone ID Related Accession (Same CDS sequence)	XM_017021615.2 , XM_017021615.1
Accession Version	XM_017021615.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	signal recognition particle 54 kDa protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017021615.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGTTG GATTGCAAGG GAGTGGTAAA ACAACAACAT GTTCAAAGCT AGCATATTAT 
TACCAGAGGA AAGGTTGGAA GACCTGTTTA ATATGTGCAG ACACATTCAG AGCAGGGGCT 
TTTGACCAAC TAAAACAGAA TGCTACCAAA GCAAGAATTC CATTTTATGG AAGCTATACA 
GAAATGGATC CTGTCATCAT TGCTTCTGAA GGAGTAGAGA AATTTAAAAA TGAAAATTTT 
GAAATTATTA TTGTTGATAC AAGTGGCCGC CACAAACAAG AAGACTCTTT GTTTGAAGAA 
ATGCTTCAAG TTGCTAATGC TATACAACCT GATAACATTG TTTATGTGAT GGATGCCTCC 
ATTGGGCAGG CTTGTGAAGC CCAGGCTAAG GCTTTTAAAG ATAAAGTAGA TGTAGCCTCA 
GTAATAGTGA CAAAACTTGA TGGCCATGCA AAAGGAGGTG GTGCACTCAG TGCAGTCGCT 
GCCACAAAAA GTCCGATTAT TTTCATTGGT ACAGGGGAAC ATATAGATGA CTTTGAACCT 
TTCAAAACAC AGCCTTTTAT TAGCAAACTT CTTGGTATGG GCGACATTGA AGGACTGATA 
GATAAAGTCA ACGAGTTGAA GTTGGATGAC AATGAAGCAC TTATAGAGAA GTTGAAACAT 
GGTCAGTTTA CGTTGCGAGA CATGTATGAG CAATTTCAAA ATATCATGAA AATGGGCCCC 
TTCAGTCAGA TCTTGGGGAT GATCCCTGGT TTTGGGACAG ATTTTATGAG CAAAGGAAAT 
GAACAGGAGT CAATGGCAAG GCTAAAGAAA TTAATGACAA TAATGGATAG TATGAATGAT 
CAAGAACTAG ACAGTACGGA TGGTGCCAAA GTTTTTAGTA AACAACCAGG AAGAATCCAA 
AGAGTAGCAA GAGGATCGGG TGTATCAACA AGAGATGTTC AAGAACTTTT GACACAATAT 
ACCAAGTTTG CACAGATGGT AAAAAAGATG GGAGGTATCA AAGGACTTTT CAAAGGTGGC 
GACATGTCTA AGAATGTGAG CCAGTCACAG ATGGCAAAAT TGAACCAACA AATGGCCAAA 
ATGATGGATC CTAGGGTTCT TCATCACATG GGTGGTATGG CAGGACTTCA GTCAATGATG 
AGGCAGTTTC AACAGGGTGC TGCTGGCAAC ATGAAAGGCA TGATGGGATT CAATAATATG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877104.1
CDS	485..1687
Translation	MFVGLQGSGKTTTCSKLAYYYQRKGWKTCLICADTFRAGAFDQLKQNATKARIPFYGSYTEMDPVIIASEGVEKFKNENFEIIIVDTSGRHKQEDSLFEEMLQVANAIQPDNIVYVMDASIGQACEAQAKAFKDKVDVASVIVTKLDGHAKGGGALSAVAATKSPIIFIGTGEHIDDFEPFKTQPFISKLLGMGDIEGLIDKVNELKLDDNEALIEKLKHGQFTLRDMYEQFQNIMKMGPFSQILGMIPGFGTDFMSKGNEQESMARLKKLMTIMDSMNDQELDSTDGAKVFSKQPGRIQRVARGSGVSTRDVQELLTQYTKFAQMVKKMGGIKGLFKGGDMSKNVSQSQMAKLNQQMAKMMDPRVLHHMGGMAGLQSMMRQFQQGAAGNMKGMMGFNNM

Target ORF information:

RefSeq Version	XM_017021615.2
Organism	Homo sapiens(human)
Definition	Homo sapiens signal recognition particle 54 (SRP54), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021615.2

ORF Insert Sequence:

ATGTTTGTTG GATTGCAAGG GAGTGGTAAA ACAACAACAT GTTCAAAGCT AGCATATTAT 
TACCAGAGGA AAGGTTGGAA GACCTGTTTA ATATGTGCAG ACACATTCAG AGCAGGGGCT 
TTTGACCAAC TAAAACAGAA TGCTACCAAA GCAAGAATTC CATTTTATGG AAGCTATACA 
GAAATGGATC CTGTCATCAT TGCTTCTGAA GGAGTAGAGA AATTTAAAAA TGAAAATTTT 
GAAATTATTA TTGTTGATAC AAGTGGCCGC CACAAACAAG AAGACTCTTT GTTTGAAGAA 
ATGCTTCAAG TTGCTAATGC TATACAACCT GATAACATTG TTTATGTGAT GGATGCCTCC 
ATTGGGCAGG CTTGTGAAGC CCAGGCTAAG GCTTTTAAAG ATAAAGTAGA TGTAGCCTCA 
GTAATAGTGA CAAAACTTGA TGGCCATGCA AAAGGAGGTG GTGCACTCAG TGCAGTCGCT 
GCCACAAAAA GTCCGATTAT TTTCATTGGT ACAGGGGAAC ATATAGATGA CTTTGAACCT 
TTCAAAACAC AGCCTTTTAT TAGCAAACTT CTTGGTATGG GCGACATTGA AGGACTGATA 
GATAAAGTCA ACGAGTTGAA GTTGGATGAC AATGAAGCAC TTATAGAGAA GTTGAAACAT 
GGTCAGTTTA CGTTGCGAGA CATGTATGAG CAATTTCAAA ATATCATGAA AATGGGCCCC 
TTCAGTCAGA TCTTGGGGAT GATCCCTGGT TTTGGGACAG ATTTTATGAG CAAAGGAAAT 
GAACAGGAGT CAATGGCAAG GCTAAAGAAA TTAATGACAA TAATGGATAG TATGAATGAT 
CAAGAACTAG ACAGTACGGA TGGTGCCAAA GTTTTTAGTA AACAACCAGG AAGAATCCAA 
AGAGTAGCAA GAGGATCGGG TGTATCAACA AGAGATGTTC AAGAACTTTT GACACAATAT 
ACCAAGTTTG CACAGATGGT AAAAAAGATG GGAGGTATCA AAGGACTTTT CAAAGGTGGC 
GACATGTCTA AGAATGTGAG CCAGTCACAG ATGGCAAAAT TGAACCAACA AATGGCCAAA 
ATGATGGATC CTAGGGTTCT TCATCACATG GGTGGTATGG CAGGACTTCA GTCAATGATG 
AGGCAGTTTC AACAGGGTGC TGCTGGCAAC ATGAAAGGCA TGATGGGATT CAATAATATG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SRP54 ( XM_017021615.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877104.1 Homo sapiens signal recognition particle 54kDa (SRP54), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu60733
Clone ID Related Accession (Same CDS sequence)	XM_017021615.2 , XM_017021615.1
Accession Version	XM_017021615.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	signal recognition particle 54 kDa protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGTTG GATTGCAAGG GAGTGGTAAA ACAACAACAT GTTCAAAGCT AGCATATTAT 
TACCAGAGGA AAGGTTGGAA GACCTGTTTA ATATGTGCAG ACACATTCAG AGCAGGGGCT 
TTTGACCAAC TAAAACAGAA TGCTACCAAA GCAAGAATTC CATTTTATGG AAGCTATACA 
GAAATGGATC CTGTCATCAT TGCTTCTGAA GGAGTAGAGA AATTTAAAAA TGAAAATTTT 
GAAATTATTA TTGTTGATAC AAGTGGCCGC CACAAACAAG AAGACTCTTT GTTTGAAGAA 
ATGCTTCAAG TTGCTAATGC TATACAACCT GATAACATTG TTTATGTGAT GGATGCCTCC 
ATTGGGCAGG CTTGTGAAGC CCAGGCTAAG GCTTTTAAAG ATAAAGTAGA TGTAGCCTCA 
GTAATAGTGA CAAAACTTGA TGGCCATGCA AAAGGAGGTG GTGCACTCAG TGCAGTCGCT 
GCCACAAAAA GTCCGATTAT TTTCATTGGT ACAGGGGAAC ATATAGATGA CTTTGAACCT 
TTCAAAACAC AGCCTTTTAT TAGCAAACTT CTTGGTATGG GCGACATTGA AGGACTGATA 
GATAAAGTCA ACGAGTTGAA GTTGGATGAC AATGAAGCAC TTATAGAGAA GTTGAAACAT 
GGTCAGTTTA CGTTGCGAGA CATGTATGAG CAATTTCAAA ATATCATGAA AATGGGCCCC 
TTCAGTCAGA TCTTGGGGAT GATCCCTGGT TTTGGGACAG ATTTTATGAG CAAAGGAAAT 
GAACAGGAGT CAATGGCAAG GCTAAAGAAA TTAATGACAA TAATGGATAG TATGAATGAT 
CAAGAACTAG ACAGTACGGA TGGTGCCAAA GTTTTTAGTA AACAACCAGG AAGAATCCAA 
AGAGTAGCAA GAGGATCGGG TGTATCAACA AGAGATGTTC AAGAACTTTT GACACAATAT 
ACCAAGTTTG CACAGATGGT AAAAAAGATG GGAGGTATCA AAGGACTTTT CAAAGGTGGC 
GACATGTCTA AGAATGTGAG CCAGTCACAG ATGGCAAAAT TGAACCAACA AATGGCCAAA 
ATGATGGATC CTAGGGTTCT TCATCACATG GGTGGTATGG CAGGACTTCA GTCAATGATG 
AGGCAGTTTC AACAGGGTGC TGCTGGCAAC ATGAAAGGCA TGATGGGATT CAATAATATG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877104.1
CDS	504..1706
Translation	MFVGLQGSGKTTTCSKLAYYYQRKGWKTCLICADTFRAGAFDQLKQNATKARIPFYGSYTEMDPVIIASEGVEKFKNENFEIIIVDTSGRHKQEDSLFEEMLQVANAIQPDNIVYVMDASIGQACEAQAKAFKDKVDVASVIVTKLDGHAKGGGALSAVAATKSPIIFIGTGEHIDDFEPFKTQPFISKLLGMGDIEGLIDKVNELKLDDNEALIEKLKHGQFTLRDMYEQFQNIMKMGPFSQILGMIPGFGTDFMSKGNEQESMARLKKLMTIMDSMNDQELDSTDGAKVFSKQPGRIQRVARGSGVSTRDVQELLTQYTKFAQMVKKMGGIKGLFKGGDMSKNVSQSQMAKLNQQMAKMMDPRVLHHMGGMAGLQSMMRQFQQGAAGNMKGMMGFNNM

Target ORF information:

RefSeq Version	XM_017021615.1
Organism	Homo sapiens(human)
Definition	Homo sapiens signal recognition particle 54kDa (SRP54), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021615.1

ORF Insert Sequence:

ATGTTTGTTG GATTGCAAGG GAGTGGTAAA ACAACAACAT GTTCAAAGCT AGCATATTAT 
TACCAGAGGA AAGGTTGGAA GACCTGTTTA ATATGTGCAG ACACATTCAG AGCAGGGGCT 
TTTGACCAAC TAAAACAGAA TGCTACCAAA GCAAGAATTC CATTTTATGG AAGCTATACA 
GAAATGGATC CTGTCATCAT TGCTTCTGAA GGAGTAGAGA AATTTAAAAA TGAAAATTTT 
GAAATTATTA TTGTTGATAC AAGTGGCCGC CACAAACAAG AAGACTCTTT GTTTGAAGAA 
ATGCTTCAAG TTGCTAATGC TATACAACCT GATAACATTG TTTATGTGAT GGATGCCTCC 
ATTGGGCAGG CTTGTGAAGC CCAGGCTAAG GCTTTTAAAG ATAAAGTAGA TGTAGCCTCA 
GTAATAGTGA CAAAACTTGA TGGCCATGCA AAAGGAGGTG GTGCACTCAG TGCAGTCGCT 
GCCACAAAAA GTCCGATTAT TTTCATTGGT ACAGGGGAAC ATATAGATGA CTTTGAACCT 
TTCAAAACAC AGCCTTTTAT TAGCAAACTT CTTGGTATGG GCGACATTGA AGGACTGATA 
GATAAAGTCA ACGAGTTGAA GTTGGATGAC AATGAAGCAC TTATAGAGAA GTTGAAACAT 
GGTCAGTTTA CGTTGCGAGA CATGTATGAG CAATTTCAAA ATATCATGAA AATGGGCCCC 
TTCAGTCAGA TCTTGGGGAT GATCCCTGGT TTTGGGACAG ATTTTATGAG CAAAGGAAAT 
GAACAGGAGT CAATGGCAAG GCTAAAGAAA TTAATGACAA TAATGGATAG TATGAATGAT 
CAAGAACTAG ACAGTACGGA TGGTGCCAAA GTTTTTAGTA AACAACCAGG AAGAATCCAA 
AGAGTAGCAA GAGGATCGGG TGTATCAACA AGAGATGTTC AAGAACTTTT GACACAATAT 
ACCAAGTTTG CACAGATGGT AAAAAAGATG GGAGGTATCA AAGGACTTTT CAAAGGTGGC 
GACATGTCTA AGAATGTGAG CCAGTCACAG ATGGCAAAAT TGAACCAACA AATGGCCAAA 
ATGATGGATC CTAGGGTTCT TCATCACATG GGTGGTATGG CAGGACTTCA GTCAATGATG 
AGGCAGTTTC AACAGGGTGC TGCTGGCAAC ATGAAAGGCA TGATGGGATT CAATAATATG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SRP54 ( NM_001146282.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001139754.1 Homo sapiens signal recognition particle 54 (SRP54), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu26546
Clone ID Related Accession (Same CDS sequence)	NM_001146282.1
Accession Version	NM_001146282.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-13
Organism	Homo sapiens(human)
Product	signal recognition particle 54 kDa protein isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA495064.1, AK300824.1 and BC000652.2. Transcript Variant: This variant (2) lacks a portion of the 5' coding region and uses a distinct translational start codon, compared to variant 1. The resulting isoform (2) has a distinct N-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.158649.1, SRR1803617.230082.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCACCAT TATCAATGAA GAGGTCTGCT ATTGATCTTG AAGAGATGGC ATCTGGTCTT 
AACAAAAGAA AAATGATTCA GCATGCTGTA TTTAAAGAAC TTGTGAAGCT TGTAGACCCT 
GGAGTTAAGG CATGGACACC CACTAAAGGA AAACAAAATG TGATTATGTT TGTTGGATTG 
CAAGGGAGTG GTAAAACAAC AACATGTTCA AAGCTAGCAT ATTATTACCA GAGGAAAGGT 
TGGAAGACCT GTTTAATATG TGCAGACACA TTCAGAGCAG GGGCTTTTGA CCAACTAAAA 
CAGAATGCTA CCAAAGCAAG AATTCCATTT TATGGAAGCT ATACAGAAAT GGATCCTGTC 
ATCATTGCTT CTGAAGGAGT AGAGAAATTT AAAAATGAAA ATTTTGAAAT TATTATTGTT 
GATACAAGTG GCCGCCACAA ACAAGAAGAC TCTTTGTTTG AAGAAATGCT TCAAGTTGCT 
AATGCTATAC AACCTGATAA CATTGTTTAT GTGATGGATG CCTCCATTGG GCAGGCTTGT 
GAAGCCCAGG CTAAGGCTTT TAAAGATAAA GTAGATGTAG CCTCAGTAAT AGTGACAAAA 
CTTGATGGCC ATGCAAAAGG AGGTGGTGCA CTCAGTGCAG TCGCTGCCAC AAAAAGTCCG 
ATTATTTTCA TTGGTACAGG GGAACATATA GATGACTTTG AACCTTTCAA AACACAGCCT 
TTTATTAGCA AACTTCTTGG TATGGGCGAC ATTGAAGGAC TGATAGATAA AGTCAACGAG 
TTGAAGTTGG ATGACAATGA AGCACTTATA GAGAAGTTGA AACATGGTCA GTTTACGTTG 
CGAGACATGT ATGAGCAATT TCAAAATATC ATGAAAATGG GCCCCTTCAG TCAGATCTTG 
GGGATGATCC CTGGTTTTGG GACAGATTTT ATGAGCAAAG GAAATGAACA GGAGTCAATG 
GCAAGGCTAA AGAAATTAAT GACAATAATG GATAGTATGA ATGATCAAGA ACTAGACAGT 
ACGGATGGTG CCAAAGTTTT TAGTAAACAA CCAGGAAGAA TCCAAAGAGT AGCAAGAGGA 
TCGGGTGTAT CAACAAGAGA TGTTCAAGAA CTTTTGACAC AATATACCAA GTTTGCACAG 
ATGGTAAAAA AGATGGGAGG TATCAAAGGA CTTTTCAAAG GTGGCGACAT GTCTAAGAAT 
GTGAGCCAGT CACAGATGGC AAAATTGAAC CAACAAATGG CCAAAATGAT GGATCCTAGG 
GTTCTTCATC ACATGGGTGG TATGGCAGGA CTTCAGTCAA TGATGAGGCA GTTTCAACAG 
GGTGCTGCTG GCAACATGAA AGGCATGATG GGATTCAATA ATATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139754.1
CDS	407..1774
Translation	MPPLSMKRSAIDLEEMASGLNKRKMIQHAVFKELVKLVDPGVKAWTPTKGKQNVIMFVGLQGSGKTTTCSKLAYYYQRKGWKTCLICADTFRAGAFDQLKQNATKARIPFYGSYTEMDPVIIASEGVEKFKNENFEIIIVDTSGRHKQEDSLFEEMLQVANAIQPDNIVYVMDASIGQACEAQAKAFKDKVDVASVIVTKLDGHAKGGGALSAVAATKSPIIFIGTGEHIDDFEPFKTQPFISKLLGMGDIEGLIDKVNELKLDDNEALIEKLKHGQFTLRDMYEQFQNIMKMGPFSQILGMIPGFGTDFMSKGNEQESMARLKKLMTIMDSMNDQELDSTDGAKVFSKQPGRIQRVARGSGVSTRDVQELLTQYTKFAQMVKKMGGIKGLFKGGDMSKNVSQSQMAKLNQQMAKMMDPRVLHHMGGMAGLQSMMRQFQQGAAGNMKGMMGFNNM

Target ORF information:

RefSeq Version	NM_001146282.1
Organism	Homo sapiens(human)
Definition	Homo sapiens signal recognition particle 54 (SRP54), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001146282.1

ORF Insert Sequence:

ATGCCACCAT TATCAATGAA GAGGTCTGCT ATTGATCTTG AAGAGATGGC ATCTGGTCTT 
AACAAAAGAA AAATGATTCA GCATGCTGTA TTTAAAGAAC TTGTGAAGCT TGTAGACCCT 
GGAGTTAAGG CATGGACACC CACTAAAGGA AAACAAAATG TGATTATGTT TGTTGGATTG 
CAAGGGAGTG GTAAAACAAC AACATGTTCA AAGCTAGCAT ATTATTACCA GAGGAAAGGT 
TGGAAGACCT GTTTAATATG TGCAGACACA TTCAGAGCAG GGGCTTTTGA CCAACTAAAA 
CAGAATGCTA CCAAAGCAAG AATTCCATTT TATGGAAGCT ATACAGAAAT GGATCCTGTC 
ATCATTGCTT CTGAAGGAGT AGAGAAATTT AAAAATGAAA ATTTTGAAAT TATTATTGTT 
GATACAAGTG GCCGCCACAA ACAAGAAGAC TCTTTGTTTG AAGAAATGCT TCAAGTTGCT 
AATGCTATAC AACCTGATAA CATTGTTTAT GTGATGGATG CCTCCATTGG GCAGGCTTGT 
GAAGCCCAGG CTAAGGCTTT TAAAGATAAA GTAGATGTAG CCTCAGTAAT AGTGACAAAA 
CTTGATGGCC ATGCAAAAGG AGGTGGTGCA CTCAGTGCAG TCGCTGCCAC AAAAAGTCCG 
ATTATTTTCA TTGGTACAGG GGAACATATA GATGACTTTG AACCTTTCAA AACACAGCCT 
TTTATTAGCA AACTTCTTGG TATGGGCGAC ATTGAAGGAC TGATAGATAA AGTCAACGAG 
TTGAAGTTGG ATGACAATGA AGCACTTATA GAGAAGTTGA AACATGGTCA GTTTACGTTG 
CGAGACATGT ATGAGCAATT TCAAAATATC ATGAAAATGG GCCCCTTCAG TCAGATCTTG 
GGGATGATCC CTGGTTTTGG GACAGATTTT ATGAGCAAAG GAAATGAACA GGAGTCAATG 
GCAAGGCTAA AGAAATTAAT GACAATAATG GATAGTATGA ATGATCAAGA ACTAGACAGT 
ACGGATGGTG CCAAAGTTTT TAGTAAACAA CCAGGAAGAA TCCAAAGAGT AGCAAGAGGA 
TCGGGTGTAT CAACAAGAGA TGTTCAAGAA CTTTTGACAC AATATACCAA GTTTGCACAG 
ATGGTAAAAA AGATGGGAGG TATCAAAGGA CTTTTCAAAG GTGGCGACAT GTCTAAGAAT 
GTGAGCCAGT CACAGATGGC AAAATTGAAC CAACAAATGG CCAAAATGAT GGATCCTAGG 
GTTCTTCATC ACATGGGTGG TATGGCAGGA CTTCAGTCAA TGATGAGGCA GTTTCAACAG 
GGTGCTGCTG GCAACATGAA AGGCATGATG GGATTCAATA ATATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SRP54 ( XM_005268024.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005268081.1 Homo sapiens signal recognition particle 54 (SRP54), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu47874
Clone ID Related Accession (Same CDS sequence)	XM_005268024.3 , XM_005268024.2
Accession Version	XM_005268024.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	signal recognition particle 54 kDa protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005268024.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCTAG CAGACCTTGG AAGAAAAATA ACATCAGCAT TACGCTCGTT GAGCAATGCC 
ACCATTATCA ATGAAGAGGT ATTGAATGCT ATGCTAAAAG AAGTCTGTAC CGCTTTGTTG 
GAAGCAGATG TTAATATTAA ACTAGTGAAG CAACTAAGAG AAAATGTTAA GTCTGCTATT 
GATCTTGAAG AGATGGCATC TGGTCTTAAC AAAAGAAAAA TGATTCAGCA TGCTGTATTT 
AAAGAACTTG TGAAGCTTGT AGACCCTGGA GTTAAGGCAT GGACACCCAC TAAAGGAAAA 
CAAAATGTGA TTATGTTTGT TGGATTGCAA GGGAGTGGTA AAACAACAAC ATGTTCAAAG 
CTAGCATATT ATTACCAGAG GAAAGGTTGG AAGACCTGTT TAATATGTGC AGACACATTC 
AGAGCAGGGG CTTTTGACCA ACTAAAACAG AATGCTACCA AAGCAAGAAT TCCATTTTAT 
GGAAGCTATA CAGAAATGGA TCCTGTCATC ATTGCTTCTG AAGGAGTAGA GAAATTTAAA 
AATGAAAATT TTGAAATTAT TATTGTTGAT ACAAGTGGCC GCCACAAACA AGAAGACTCT 
TTGTTTGAAG AAATGCTTCA AGTTGCTAAT GCTATACAAC CTGATAACAT TGTTTATGTG 
ATGGATGCCT CCATTGGGCA GGCTTGTGAA GCCCAGGCTA AGGCTTTTAA AGATAAAGTA 
GATGTAGCCT CAGTAATAGT GACAAAACTT GATGGCCATG CAAAAGGAGG TGGTGCACTC 
AGTGCAGTCG CTGCCACAAA AAGTCCGATT ATTTTCATTG GTACAGGGGA ACATATAGAT 
GACTTTGAAC CTTTCAAAAC ACAGCCTTTT ATTAGCAAAC TTCTTGGTAT GGGCGACATT 
GAAGGACTGA TAGATAAAGT CAACGAGTTG AAGTTGGATG ACAATGAAGC ACTTATAGAG 
AAGTTGAAAC ATGGTCAGTT TACGTTGCGA GACATGTATG AGCAATTTCA AAATATCATG 
AAAATGGGCC CCTTCAGTCA GATCTTGGGG ATGATCCCTG GTTTTGGGAC AGATTTTATG 
AGCAAAGGAA ATGAACAGGA GTCAATGGCA AGGCTAAAGA AATTAATGAC AATAATGGAT 
AGTATGAATG ATCAAGGTGG CGACATGTCT AAGAATGTGA GCCAGTCACA GATGGCAAAA 
TTGAACCAAC AAATGGCCAA AATGATGGAT CCTAGGGTTC TTCATCACAT GGGTGGTATG 
GCAGGACTTC AGTCAATGAT GAGGCAGTTT CAACAGGGTG CTGCTGGCAA CATGAAAGGC 
ATGATGGGAT TCAATAATAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005268081.1
CDS	269..1612
Translation	MVLADLGRKITSALRSLSNATIINEEVLNAMLKEVCTALLEADVNIKLVKQLRENVKSAIDLEEMASGLNKRKMIQHAVFKELVKLVDPGVKAWTPTKGKQNVIMFVGLQGSGKTTTCSKLAYYYQRKGWKTCLICADTFRAGAFDQLKQNATKARIPFYGSYTEMDPVIIASEGVEKFKNENFEIIIVDTSGRHKQEDSLFEEMLQVANAIQPDNIVYVMDASIGQACEAQAKAFKDKVDVASVIVTKLDGHAKGGGALSAVAATKSPIIFIGTGEHIDDFEPFKTQPFISKLLGMGDIEGLIDKVNELKLDDNEALIEKLKHGQFTLRDMYEQFQNIMKMGPFSQILGMIPGFGTDFMSKGNEQESMARLKKLMTIMDSMNDQGGDMSKNVSQSQMAKLNQQMAKMMDPRVLHHMGGMAGLQSMMRQFQQGAAGNMKGMMGFNNM

Target ORF information:

RefSeq Version	XM_005268024.3
Organism	Homo sapiens(human)
Definition	Homo sapiens signal recognition particle 54 (SRP54), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268024.3

ORF Insert Sequence:

ATGGTTCTAG CAGACCTTGG AAGAAAAATA ACATCAGCAT TACGCTCGTT GAGCAATGCC 
ACCATTATCA ATGAAGAGGT ATTGAATGCT ATGCTAAAAG AAGTCTGTAC CGCTTTGTTG 
GAAGCAGATG TTAATATTAA ACTAGTGAAG CAACTAAGAG AAAATGTTAA GTCTGCTATT 
GATCTTGAAG AGATGGCATC TGGTCTTAAC AAAAGAAAAA TGATTCAGCA TGCTGTATTT 
AAAGAACTTG TGAAGCTTGT AGACCCTGGA GTTAAGGCAT GGACACCCAC TAAAGGAAAA 
CAAAATGTGA TTATGTTTGT TGGATTGCAA GGGAGTGGTA AAACAACAAC ATGTTCAAAG 
CTAGCATATT ATTACCAGAG GAAAGGTTGG AAGACCTGTT TAATATGTGC AGACACATTC 
AGAGCAGGGG CTTTTGACCA ACTAAAACAG AATGCTACCA AAGCAAGAAT TCCATTTTAT 
GGAAGCTATA CAGAAATGGA TCCTGTCATC ATTGCTTCTG AAGGAGTAGA GAAATTTAAA 
AATGAAAATT TTGAAATTAT TATTGTTGAT ACAAGTGGCC GCCACAAACA AGAAGACTCT 
TTGTTTGAAG AAATGCTTCA AGTTGCTAAT GCTATACAAC CTGATAACAT TGTTTATGTG 
ATGGATGCCT CCATTGGGCA GGCTTGTGAA GCCCAGGCTA AGGCTTTTAA AGATAAAGTA 
GATGTAGCCT CAGTAATAGT GACAAAACTT GATGGCCATG CAAAAGGAGG TGGTGCACTC 
AGTGCAGTCG CTGCCACAAA AAGTCCGATT ATTTTCATTG GTACAGGGGA ACATATAGAT 
GACTTTGAAC CTTTCAAAAC ACAGCCTTTT ATTAGCAAAC TTCTTGGTAT GGGCGACATT 
GAAGGACTGA TAGATAAAGT CAACGAGTTG AAGTTGGATG ACAATGAAGC ACTTATAGAG 
AAGTTGAAAC ATGGTCAGTT TACGTTGCGA GACATGTATG AGCAATTTCA AAATATCATG 
AAAATGGGCC CCTTCAGTCA GATCTTGGGG ATGATCCCTG GTTTTGGGAC AGATTTTATG 
AGCAAAGGAA ATGAACAGGA GTCAATGGCA AGGCTAAAGA AATTAATGAC AATAATGGAT 
AGTATGAATG ATCAAGGTGG CGACATGTCT AAGAATGTGA GCCAGTCACA GATGGCAAAA 
TTGAACCAAC AAATGGCCAA AATGATGGAT CCTAGGGTTC TTCATCACAT GGGTGGTATG 
GCAGGACTTC AGTCAATGAT GAGGCAGTTT CAACAGGGTG CTGCTGGCAA CATGAAAGGC 
ATGATGGGAT TCAATAATAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SRP54 ( XM_005268024.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005268081.1 Homo sapiens signal recognition particle 54kDa (SRP54), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu47874
Clone ID Related Accession (Same CDS sequence)	XM_005268024.3 , XM_005268024.2
Accession Version	XM_005268024.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	signal recognition particle 54 kDa protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005268024.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCTAG CAGACCTTGG AAGAAAAATA ACATCAGCAT TACGCTCGTT GAGCAATGCC 
ACCATTATCA ATGAAGAGGT ATTGAATGCT ATGCTAAAAG AAGTCTGTAC CGCTTTGTTG 
GAAGCAGATG TTAATATTAA ACTAGTGAAG CAACTAAGAG AAAATGTTAA GTCTGCTATT 
GATCTTGAAG AGATGGCATC TGGTCTTAAC AAAAGAAAAA TGATTCAGCA TGCTGTATTT 
AAAGAACTTG TGAAGCTTGT AGACCCTGGA GTTAAGGCAT GGACACCCAC TAAAGGAAAA 
CAAAATGTGA TTATGTTTGT TGGATTGCAA GGGAGTGGTA AAACAACAAC ATGTTCAAAG 
CTAGCATATT ATTACCAGAG GAAAGGTTGG AAGACCTGTT TAATATGTGC AGACACATTC 
AGAGCAGGGG CTTTTGACCA ACTAAAACAG AATGCTACCA AAGCAAGAAT TCCATTTTAT 
GGAAGCTATA CAGAAATGGA TCCTGTCATC ATTGCTTCTG AAGGAGTAGA GAAATTTAAA 
AATGAAAATT TTGAAATTAT TATTGTTGAT ACAAGTGGCC GCCACAAACA AGAAGACTCT 
TTGTTTGAAG AAATGCTTCA AGTTGCTAAT GCTATACAAC CTGATAACAT TGTTTATGTG 
ATGGATGCCT CCATTGGGCA GGCTTGTGAA GCCCAGGCTA AGGCTTTTAA AGATAAAGTA 
GATGTAGCCT CAGTAATAGT GACAAAACTT GATGGCCATG CAAAAGGAGG TGGTGCACTC 
AGTGCAGTCG CTGCCACAAA AAGTCCGATT ATTTTCATTG GTACAGGGGA ACATATAGAT 
GACTTTGAAC CTTTCAAAAC ACAGCCTTTT ATTAGCAAAC TTCTTGGTAT GGGCGACATT 
GAAGGACTGA TAGATAAAGT CAACGAGTTG AAGTTGGATG ACAATGAAGC ACTTATAGAG 
AAGTTGAAAC ATGGTCAGTT TACGTTGCGA GACATGTATG AGCAATTTCA AAATATCATG 
AAAATGGGCC CCTTCAGTCA GATCTTGGGG ATGATCCCTG GTTTTGGGAC AGATTTTATG 
AGCAAAGGAA ATGAACAGGA GTCAATGGCA AGGCTAAAGA AATTAATGAC AATAATGGAT 
AGTATGAATG ATCAAGGTGG CGACATGTCT AAGAATGTGA GCCAGTCACA GATGGCAAAA 
TTGAACCAAC AAATGGCCAA AATGATGGAT CCTAGGGTTC TTCATCACAT GGGTGGTATG 
GCAGGACTTC AGTCAATGAT GAGGCAGTTT CAACAGGGTG CTGCTGGCAA CATGAAAGGC 
ATGATGGGAT TCAATAATAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005268081.1
CDS	605..1948
Translation	MVLADLGRKITSALRSLSNATIINEEVLNAMLKEVCTALLEADVNIKLVKQLRENVKSAIDLEEMASGLNKRKMIQHAVFKELVKLVDPGVKAWTPTKGKQNVIMFVGLQGSGKTTTCSKLAYYYQRKGWKTCLICADTFRAGAFDQLKQNATKARIPFYGSYTEMDPVIIASEGVEKFKNENFEIIIVDTSGRHKQEDSLFEEMLQVANAIQPDNIVYVMDASIGQACEAQAKAFKDKVDVASVIVTKLDGHAKGGGALSAVAATKSPIIFIGTGEHIDDFEPFKTQPFISKLLGMGDIEGLIDKVNELKLDDNEALIEKLKHGQFTLRDMYEQFQNIMKMGPFSQILGMIPGFGTDFMSKGNEQESMARLKKLMTIMDSMNDQGGDMSKNVSQSQMAKLNQQMAKMMDPRVLHHMGGMAGLQSMMRQFQQGAAGNMKGMMGFNNM

Target ORF information:

RefSeq Version	XM_005268024.2
Organism	Homo sapiens(human)
Definition	Homo sapiens signal recognition particle 54kDa (SRP54), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268024.2

ORF Insert Sequence:

ATGGTTCTAG CAGACCTTGG AAGAAAAATA ACATCAGCAT TACGCTCGTT GAGCAATGCC 
ACCATTATCA ATGAAGAGGT ATTGAATGCT ATGCTAAAAG AAGTCTGTAC CGCTTTGTTG 
GAAGCAGATG TTAATATTAA ACTAGTGAAG CAACTAAGAG AAAATGTTAA GTCTGCTATT 
GATCTTGAAG AGATGGCATC TGGTCTTAAC AAAAGAAAAA TGATTCAGCA TGCTGTATTT 
AAAGAACTTG TGAAGCTTGT AGACCCTGGA GTTAAGGCAT GGACACCCAC TAAAGGAAAA 
CAAAATGTGA TTATGTTTGT TGGATTGCAA GGGAGTGGTA AAACAACAAC ATGTTCAAAG 
CTAGCATATT ATTACCAGAG GAAAGGTTGG AAGACCTGTT TAATATGTGC AGACACATTC 
AGAGCAGGGG CTTTTGACCA ACTAAAACAG AATGCTACCA AAGCAAGAAT TCCATTTTAT 
GGAAGCTATA CAGAAATGGA TCCTGTCATC ATTGCTTCTG AAGGAGTAGA GAAATTTAAA 
AATGAAAATT TTGAAATTAT TATTGTTGAT ACAAGTGGCC GCCACAAACA AGAAGACTCT 
TTGTTTGAAG AAATGCTTCA AGTTGCTAAT GCTATACAAC CTGATAACAT TGTTTATGTG 
ATGGATGCCT CCATTGGGCA GGCTTGTGAA GCCCAGGCTA AGGCTTTTAA AGATAAAGTA 
GATGTAGCCT CAGTAATAGT GACAAAACTT GATGGCCATG CAAAAGGAGG TGGTGCACTC 
AGTGCAGTCG CTGCCACAAA AAGTCCGATT ATTTTCATTG GTACAGGGGA ACATATAGAT 
GACTTTGAAC CTTTCAAAAC ACAGCCTTTT ATTAGCAAAC TTCTTGGTAT GGGCGACATT 
GAAGGACTGA TAGATAAAGT CAACGAGTTG AAGTTGGATG ACAATGAAGC ACTTATAGAG 
AAGTTGAAAC ATGGTCAGTT TACGTTGCGA GACATGTATG AGCAATTTCA AAATATCATG 
AAAATGGGCC CCTTCAGTCA GATCTTGGGG ATGATCCCTG GTTTTGGGAC AGATTTTATG 
AGCAAAGGAA ATGAACAGGA GTCAATGGCA AGGCTAAAGA AATTAATGAC AATAATGGAT 
AGTATGAATG ATCAAGGTGG CGACATGTCT AAGAATGTGA GCCAGTCACA GATGGCAAAA 
TTGAACCAAC AAATGGCCAA AATGATGGAT CCTAGGGTTC TTCATCACAT GGGTGGTATG 
GCAGGACTTC AGTCAATGAT GAGGCAGTTT CAACAGGGTG CTGCTGGCAA CATGAAAGGC 
ATGATGGGAT TCAATAATAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutations in signal recognition particle SRP54 cause syndromic neutropenia with Shwachman-Diamond-like features.
The Journal of clinical investigation127(11)4090-4103(2017 Nov)
Carapito R,Konantz M,Paillard C,Miao Z,Pichot A,Leduc MS,Yang Y,Bergstrom KL,Mahoney DH,Shardy DL,Alsaleh G,Naegely L,Kolmer A,Paul N,Hanauer A,Rolli V,M?ller JS,Alghisi E,Sauteur L,Macquin C,Morlon A,Sancho CS,Amati-Bonneau P,Procaccio V,Mosca-Boidron AL,Marle N,Osmani N,Lefebvre O,Goetz JG,Unal S,Akarsu NA,Radosavljevic M,Chenard MP,Rialland F,Grain A,B?n? MC,Eveillard M,Vincent M,Guy J,Faivre L,Thauvin-Robinet C,Thevenon J,Myers K,Fleming MD,Shimamura A,Bottollier-Lemallaz E,Westhof E,Lengerke C,Isidor B,Bahram S

Host factors that interact with the pestivirus N-terminal protease, Npro, are components of the ribonucleoprotein complex.
Journal of virology88(18)10340-53(2014 Sep)
Jefferson M,Donaszi-Ivanov A,Pollen S,Dalmay T,Saalbach G,Powell PP

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

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SRP54 cDNA ORF clone, Homo sapiens(human)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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