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BRAF cDNA ORF clone, Homo sapiens (human)

Gene Symbol BRAF
Entrez Gene ID 673
Full Name B-Raf proto-oncogene, serine/threonine kinase
Synonyms B-RAF1, BRAF1, NS7, RAFB1
General protein information
Preferred Names
serine/threonine-protein kinase B-raf
Names
serine/threonine-protein kinase B-raf
94 kDa B-raf protein
proto-oncogene B-Raf
v-raf murine sarcoma viral oncogene homolog B1
murine sarcoma viral (v-raf) oncogene homolog B1
B-Raf proto-oncogene serine/threonine-protein kinase (p94)
NP_004324.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

7

7q34

Summary This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

164757

Disorder Html: Melanoma, malignant, somatic (3); Colorectal cancer, somatic (3);

mRNA and Protein(s)

mRNA Protein Name
XM_005250045 XP_005250102 serine/threonine-protein kinase B-raf isoform X1
XM_005250046 XP_005250103 serine/threonine-protein kinase B-raf isoform X2
XM_011516529 XP_011514831 serine/threonine-protein kinase B-raf isoform X3
XM_011516530 XP_011514832 serine/threonine-protein kinase B-raf isoform X4
NM_004333 NP_004324 serine/threonine-protein kinase B-raf



Homo sapiens (human) BRAF NP_004324.2
Pan troglodytes (chimpanzee) BRAF XP_003951208.1
Macaca mulatta (Rhesus monkey) LOC693554 XP_002803541.1
Canis lupus familiaris (dog) BRAF XP_005629607.1
Bos taurus (cattle) BRAF XP_005205968.1
Mus musculus (house mouse) Braf NP_647455.3
Rattus norvegicus (Norway rat) Braf XP_006236418.1
Gallus gallus (chicken) BRAF NP_990633.1
Danio rerio (zebrafish) braf NP_991307.2
Drosophila melanogaster (fruit fly) phl NP_525047.1
Caenorhabditis elegans lin-45 NP_741430.3
Xenopus (Silurana) tropicalis (western clawed frog) braf XP_004912520.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BRAF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRAF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu41737 XM_005250045 PREDICTED: Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu41738 XM_005250046 PREDICTED: Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu56517 XM_011516529 PREDICTED: Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $391.30
$559.00
OHu56518 XM_011516530 PREDICTED: Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $307.30
$439.00
OHu28570
NM_004333 Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$279.50-$391.30
$559.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu41737
Clone ID Related Accession (Same CDS sequence) XM_005250045
Accession Version XM_005250045.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2304bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product serine/threonine-protein kinase B-raf isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007933.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC
CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT
ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG
GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG
GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT
ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT
CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC
CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT
CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT
CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA
GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA
ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC
TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT
GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA
CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA
CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT
CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA
GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT
GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGGAT CAACCACAGG TTTGTCTGCT
ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA
GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA
CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA
CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG
GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT
GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC
ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT
CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT
GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT
AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG
AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG
GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT
GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC
AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG
GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA
AGAGATGAGA GACCACTCTT TCCCCAAATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA
TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG TTTCCAAACA
GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATCCAGGC AGGGGGATAT
GGAGAATTTG CAGCCTTCAA GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005250102.1
CDS9..2312
Misc Feature(1)474..689(+)
Misc Feature(2)480..575(+)
Misc Feature(3)711..848(+)
Misc Feature(4)711..848(+)
Misc Feature(5)732..779(+)
Misc Feature(6)741..749(+)
Misc Feature(7)1377..2147(+)
Misc Feature(8)1395..2153(+)
Misc Feature(9)1395..1862(+)
Misc Feature(10)1395..1790(+)
Misc Feature(11)1407..1862(+)
Misc Feature(12)1431..2153(+)
Misc Feature(13)1785..1862(+)
Translation

Target ORF information:

RefSeq Version XM_005250045
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005250045

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC
CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT
ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG
GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG
GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT
ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT
CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC
CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT
CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT
CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA
GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA
ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC
TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT
GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA
CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA
CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT
CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA
GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT
GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGGAT CAACCACAGG TTTGTCTGCT
ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA
GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA
CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA
CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG
GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT
GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC
ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT
CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT
GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT
AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG
AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG
GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT
GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC
AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG
GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA
AGAGATGAGA GACCACTCTT TCCCCAAATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA
TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG TTTCCAAACA
GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATCCAGGC AGGGGGATAT
GGAGAATTTG CAGCCTTCAA GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu41738
Clone ID Related Accession (Same CDS sequence) XM_005250046
Accession Version XM_005250046.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2277bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product serine/threonine-protein kinase B-raf isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007933.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC
CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT
ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG
GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG
GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT
ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT
CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC
CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT
CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT
CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA
GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA
ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC
TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT
GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA
CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA
CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT
CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA
GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT
GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGGAT CAACCACAGG TTTGTCTGCT
ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA
GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA
CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA
CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG
GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT
GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC
ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT
CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT
GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT
AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG
AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG
GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT
GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC
AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG
GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA
AGAGATGAGA GACCACTCTT TCCCCAAGAG AATTTGCAGC CTTCAAGTAG CCACCATCAT
GGCAGCATCT GCTCTTATTT CTTAAGTCTT GTGTTCGTAC AATTTGTTAA CATCAAAACA
CAGTTCTGTT CCTCAAATCT TTTTTTAAAG ATACAAAATT TCCAATGCAT AAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005250103.1
CDS9..2285
Misc Feature(1)474..689(+)
Misc Feature(2)480..575(+)
Misc Feature(3)711..848(+)
Misc Feature(4)711..848(+)
Misc Feature(5)732..779(+)
Misc Feature(6)741..749(+)
Misc Feature(7)1377..2135(+)
Misc Feature(8)1395..2135(+)
Misc Feature(9)1395..1862(+)
Misc Feature(10)1395..1790(+)
Misc Feature(11)1407..1862(+)
Misc Feature(12)1431..1772(+)
Misc Feature(13)1785..1862(+)
Translation

Target ORF information:

RefSeq Version XM_005250046
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005250046

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC
CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT
ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG
GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG
GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT
ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT
CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC
CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT
CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT
CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA
GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA
ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC
TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT
GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA
CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA
CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT
CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA
GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT
GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGGAT CAACCACAGG TTTGTCTGCT
ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA
GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA
CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA
CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG
GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT
GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC
ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT
CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT
GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT
AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG
AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG
GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT
GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC
AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG
GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA
AGAGATGAGA GACCACTCTT TCCCCAAGAG AATTTGCAGC CTTCAAGTAG CCACCATCAT
GGCAGCATCT GCTCTTATTT CTTAAGTCTT GTGTTCGTAC AATTTGTTAA CATCAAAACA
CAGTTCTGTT CCTCAAATCT TTTTTTAAAG ATACAAAATT TCCAATGCAT AAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56517
Clone ID Related Accession (Same CDS sequence) XM_011516529
Accession Version XM_011516529.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2136bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product serine/threonine-protein kinase B-raf isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007933.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC
CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT
ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG
GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG
GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT
ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT
CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC
CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT
CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT
CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA
GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA
ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC
TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT
GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA
CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA
CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT
CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA
GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT
GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGGAT CAACCACAGG TTTGTCTGCT
ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA
GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA
CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA
CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG
GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT
GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC
ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT
CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT
GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT
AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG
AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG
GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT
GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC
AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG
GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA
AGAGATGAGA GACCACTCTT TCCCCAAGTA ATATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011514831.1
CDS9..2144
Misc Feature(1)474..689(+)
Misc Feature(2)480..575(+)
Misc Feature(3)711..848(+)
Misc Feature(4)711..848(+)
Misc Feature(5)732..779(+)
Misc Feature(6)741..749(+)
Misc Feature(7)1377..2138(+)
Misc Feature(8)1395..2138(+)
Misc Feature(9)1395..1862(+)
Misc Feature(10)1395..1790(+)
Misc Feature(11)1407..1862(+)
Misc Feature(12)1431..1772(+)
Misc Feature(13)1785..1862(+)
Translation

Target ORF information:

RefSeq Version XM_011516529
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011516529

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC
CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT
ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG
GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG
GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT
ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT
CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC
CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT
CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT
CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA
GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA
ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC
TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT
GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA
CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA
CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT
CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA
GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT
GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGGAT CAACCACAGG TTTGTCTGCT
ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA
GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA
CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA
CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG
GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT
GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC
ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT
CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT
GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT
AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG
AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG
GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT
GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC
AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG
GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA
AGAGATGAGA GACCACTCTT TCCCCAAGTA ATATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56518
Clone ID Related Accession (Same CDS sequence) XM_011516530
Accession Version XM_011516530.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1782bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product serine/threonine-protein kinase B-raf isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007933.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC
CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT
ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG
GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG
GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT
ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT
CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC
CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT
CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT
CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA
GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA
ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC
TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT
GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA
CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA
CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT
CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA
GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT
GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGGAT CAACCACAGG TTTGTCTGCT
ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA
GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA
CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA
CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG
GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT
GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC
ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT
CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT
GCACAGGGCA TGGAGCACCA GAAGTCATCA GAATGCAAGA TAAAAATCCA TACAGCTTTC
AGTCAGATGT ATATGCATTT GGAATTGTTC TGTATGAATT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011514832.1
CDS9..1790
Misc Feature(1)474..689(+)
Misc Feature(2)480..575(+)
Misc Feature(3)711..848(+)
Misc Feature(4)711..848(+)
Misc Feature(5)732..779(+)
Misc Feature(6)741..749(+)
Misc Feature(7)1350..>1715(+)
Misc Feature(8)1395..1604(+)
Translation

Target ORF information:

RefSeq Version XM_011516530
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011516530

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC
CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT
ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG
GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG
GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT
ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT
CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC
CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT
CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT
CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA
GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA
ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC
TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT
GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA
CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA
CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT
CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA
GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT
GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGGAT CAACCACAGG TTTGTCTGCT
ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA
GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA
CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA
CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG
GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT
GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC
ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT
CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT
GCACAGGGCA TGGAGCACCA GAAGTCATCA GAATGCAAGA TAAAAATCCA TACAGCTTTC
AGTCAGATGT ATATGCATTT GGAATTGTTC TGTATGAATT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28570
Clone ID Related Accession (Same CDS sequence) NM_004333
Accession Version NM_004333.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2301bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product serine/threonine-protein kinase B-raf
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB454480.1, BC101757.1, M95712.2, AW971583.1 and AA252358.1. This sequence is a reference standard in the RefSeqGene project. On May 7, 2008 this sequence version replaced gi:90265828. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M95712.2, BC101757.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC
CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT
ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG
GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG
GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT
ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT
CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC
CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT
CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT
CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA
GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA
ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC
TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT
GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA
CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA
CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT
CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA
GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT
GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGGAT CAACCACAGG TTTGTCTGCT
ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA
GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA
CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA
CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG
GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT
GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC
ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT
CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT
GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT
AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG
AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG
GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT
GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC
AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG
GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA
AGAGATGAGA GACCACTCTT TCCCCAAATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA
TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG TTTCCAAACA
GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATCCAGGC AGGGGGATAT
GGTGCGTTTC CTGTCCACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_004324.2
CDS62..2362
Misc Feature(1)65..67(+)
Misc Feature(2)416..418(+)
Misc Feature(3)500..502(+)
Misc Feature(4)512..514(+)
Misc Feature(5)527..742(+)
Misc Feature(6)533..628(+)
Misc Feature(7)764..901(+)
Misc Feature(8)764..901(+)
Misc Feature(9)785..832(+)
Misc Feature(10)794..802(+)
Misc Feature(11)1154..1156(+)
Misc Feature(12)1154..1156(+)
Misc Feature(13)1154..1156(+)
Misc Feature(14)1178..1180(+)
Misc Feature(15)1178..1180(+)
Misc Feature(16)1199..1204(+)
Misc Feature(17)1247..1249(+)
Misc Feature(18)1256..1258(+)
Misc Feature(19)1262..1264(+)
Misc Feature(20)1262..1264(+)
Misc Feature(21)1316..1318(+)
Misc Feature(22)1346..1348(+)
Misc Feature(23)1349..1351(+)
Misc Feature(24)1373..1378(+)
Misc Feature(25)1379..1381(+)
Misc Feature(26)1397..1399(+)
Misc Feature(27)1397..1399(+)
Misc Feature(28)1397..1399(+)
Misc Feature(29)1400..1402(+)
Misc Feature(30)1430..2200(+)
Misc Feature(31)1448..2206(+)
Misc Feature(32)1448..1915(+)
Misc Feature(33)1448..1843(+)
Misc Feature(34)1460..1915(+)
Misc Feature(35)1484..2206(+)
Misc Feature(36)1838..1915(+)
Misc Feature(37)1856..1858(+)
Misc Feature(38)1865..1867(+)
Misc Feature(39)2072..2074(+)
Misc Feature(40)2246..2248(+)
Misc Feature(41)2246..2248(+)
Misc Feature(42)2309..2311(+)
Misc Feature(43)2318..2320(+)
Exon (1)1..199
Gene:BRAF
Gene Synonym:
Exon (2)200..301
Gene:BRAF
Gene Synonym:
Exon (3)302..565
Gene:BRAF
Gene Synonym:
Exon (4)566..669
Gene:BRAF
Gene Synonym:
Exon (5)670..772
Gene:BRAF
Gene Synonym:
Exon (6)773..921
Gene:BRAF
Gene Synonym:
Exon (7)922..1041
Gene:BRAF
Gene Synonym:
Exon (8)1042..1201
Gene:BRAF
Gene Synonym:
Exon (9)1202..1238
Gene:BRAF
Gene Synonym:
Exon (10)1239..1375
Gene:BRAF
Gene Synonym:
Exon (11)1376..1493
Gene:BRAF
Gene Synonym:
Exon (12)1494..1578
Gene:BRAF
Gene Synonym:
Exon (13)1579..1755
Gene:BRAF
Gene Synonym:
Exon (14)1756..1802
Gene:BRAF
Gene Synonym:
Exon (15)1803..1921
Gene:BRAF
Gene Synonym:
Exon (16)1922..2053
Gene:BRAF
Gene Synonym:
Exon (17)2054..2188
Gene:BRAF
Gene Synonym:
Exon (18)2189..2947
Gene:BRAF
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_004333
Organism Homo sapiens (human)
Definition Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_004333

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC
CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT
ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG
GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG
GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT
ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT
CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC
CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT
CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT
CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA
GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA
ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC
TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT
GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA
CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA
CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT
CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA
GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT
GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGGAT CAACCACAGG TTTGTCTGCT
ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA
GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA
CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA
CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG
GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT
GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC
ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT
CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT
GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT
AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG
AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG
GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT
GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC
AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG
GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA
AGAGATGAGA GACCACTCTT TCCCCAAATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA
TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG TTTCCAAACA
GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATCCAGGC AGGGGGATAT
GGTGCGTTTC CTGTCCACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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