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BRAF cDNA ORF clone, Homo sapiens(Human)

Gene Symbol BRAF
Entrez Gene ID 673
Full Name B-Raf proto-oncogene, serine/threonine kinase
Synonyms B-RAF1,B-raf,BRAF1,NS7,RAFB1
General protein information
Preferred Names

B-Raf proto-oncogene, serine/threonine kinase

Names

serine/threonine-protein kinase B-raf
94 kDa B-raf protein
B-Raf proto-oncogene serine/threonine-protein kinase (p94)
B-Raf serine/threonine-protein
murine sarcoma viral (v-raf) oncogene homolog B1
proto-oncogene B-Raf
v-raf murine sarcoma viral oncogene homolog B
v-raf murine sarcoma viral oncogene homolog B1

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

7

7q34

Summary This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:

164757

Disorder Html: Melanoma, malignant, somatic (3); Colorectal cancer, somatic (3);

mRNA and Protein(s)

mRNA Protein Name
XM_005250045.2 XP_005250102.1 serine/threonine-protein kinase B-raf isoform X3
NM_004333.4 NP_004324.2 serine/threonine-protein kinase B-raf
XM_017012558.1 XP_016868047.1 serine/threonine-protein kinase B-raf isoform X1
XM_017012559.1 XP_016868048.1 serine/threonine-protein kinase B-raf isoform X2



Mus musculus (house mouse) Braf NP_647455.3
Rattus norvegicus (Norway rat) Braf XP_006236418.1
Gallus gallus (chicken) BRAF NP_990633.1
Caenorhabditis elegans (roundworm) lin-45 NP_741430.3
Pan troglodytes (chimpanzee) BRAF XP_003951208.1
Bos taurus (cattle) BRAF XP_005205968.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP004699 XP_318144.4
Macaca mulatta (rhesus monkey) LOC693554 XP_002803541.1
Canis lupus familiaris (dog) BRAF XP_005629607.1
Drosophila melanogaster (fruit fly) phl NP_525047.1
Xenopus tropicalis (tropical clawed frog) braf XP_004912520.1
Danio rerio (zebrafish) braf NP_991307.2
Homo sapiens (human) BRAF NP_004324.2


Related articles in PubMed

A meta-analysis of the association between BRAF mutation and nonsmall cell lung cancer.
Cui G, Liu D, Li W, Fu X, Liang Y, Li Y, Shi W, Chen X, Zhao S
Medicine96(14)e6552(2017 Apr)

BRaf signaling principles unveiled by large-scale human mutation analysis with a rapid lentivirus-based gene replacement method.
Lim CS, Kang X, Mirabella V, Zhang H, Bu Q, Araki Y, Hoang ET, Wang S, Shen Y, Choi S, Kaang BK, Chang Q, Pang ZP, Huganir RL, Zhu JJ
Genes & development31(6)537-552(2017 Mar)

Evaluation and identification of factors related to
Lin L, Chen GY, Xu CW, Wang HY, Wu YF, Fang MY
Journal of cancer research and therapeutics12(Supplement)C191-C198(2016 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The percentage of mutant BRAF alleles in papillary thyroid carcinoma is significantly associated with tumor burden and extrathyroidal invasion.
Title: Significance of allelic percentage of BRAF c.1799T > A (V600E) mutation in papillary thyroid carcinoma.

the BRAF pathway may contribute to the pathogenesis or malignant transformation of histiocytic and dendritic cell neoplasms.
Title: Frequent detection of BRAF(V600E) mutations in histiocytic and dendritic cell neoplasms.

BRAF mutation is associated with melanoma.
Title: Correlation of somatic mutations and clinical outcome in melanoma patients treated with Carboplatin, Paclitaxel, and sorafenib.

Response to this therapy suggests that BRAF inhibitors can affect primary CNS lesions when a documented and targetable mutation is present.
Title: Complete clinical regression of a BRAF V600E-mutant pediatric glioblastoma multiforme after BRAF inhibitor therapy.

AKTi combined with BRAFi-based therapy may benefit patients with tumors harboring BRAF mutations and particularly PTEN deletions or AKT mutations.
Title: Effects of AKT inhibitor therapy in response and resistance to BRAF inhibition in melanoma.

The following BRAF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRAF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu28570
NM_004333.4
Latest version!
Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$279.50-$391.30
$559.00
OHu41737 XM_005250045.2
Latest version!
Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu97466 XM_017012558.1
Latest version!
Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00
OHu97467 XM_017012559.1
Latest version!
Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu28570
Clone ID Related Accession (Same CDS sequence) NM_004333.4
Accession Version NM_004333.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2301bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Homo sapiens(Human)
Product serine/threonine-protein kinase B-raf
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB454480.1, BC101757.1, M95712.2, AW971583.1 and AA252358.1. This sequence is a reference standard in the RefSeqGene project. On May 7, 2008 this sequence version replaced NM_004333.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M95712.2, BC101757.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC
CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT
ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG
GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG
GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT
ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT
CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC
CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT
CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT
CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA
GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA
ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC
TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT
GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA
CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA
CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT
CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA
GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT
GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGGAT CAACCACAGG TTTGTCTGCT
ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA
GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA
CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA
CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG
GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT
GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC
ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT
CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT
GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT
AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG
AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG
GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT
GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC
AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG
GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA
AGAGATGAGA GACCACTCTT TCCCCAAATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA
TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG TTTCCAAACA
GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATCCAGGC AGGGGGATAT
GGTGCGTTTC CTGTCCACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_004324.2
CDS62..2362
Misc Feature(1)65..67
Misc Feature(2)416..418
Misc Feature(3)500..502
Misc Feature(4)512..514
Misc Feature(5)512..514
Misc Feature(6)527..742
Misc Feature(7)order(533..535,548..550,554..565,569..571,608..610,620..628)
Misc Feature(8)764..901
Misc Feature(9)order(764..766,803..805,812..814,842..844,851..853,866..868,875..877,899..901)
Misc Feature(10)order(794..796,800..802)
Misc Feature(11)1058..1060
Misc Feature(12)1154..1156
Misc Feature(13)1154..1156
Misc Feature(14)1154..1156
Misc Feature(15)1178..1180
Misc Feature(16)1178..1180
Misc Feature(17)1199..1204
Misc Feature(18)1247..1249
Misc Feature(19)1256..1258
Misc Feature(20)1262..1264
Misc Feature(21)1262..1264
Misc Feature(22)1316..1318
Misc Feature(23)1346..1348
Misc Feature(24)1349..1351
Misc Feature(25)1373..1378
Misc Feature(26)1379..1381
Misc Feature(27)1397..1399
Misc Feature(28)1397..1399
Misc Feature(29)1397..1399
Misc Feature(30)1400..1402
Misc Feature(31)1433..2200
Misc Feature(32)1448..2206
Misc Feature(33)order(1448..1462,1472..1474,1502..1504,1508..1510,1601..1603,1646..1657,1667..1669,1673..1675,1787..1789,1793..1795,1799..1804,1808..1810,1841..1843,1850..1852,1904..1915)
Misc Feature(34)order(1448..1462,1472..1474,1502..1504,1508..1510,1601..1603,1646..1657,1667..1669,1787..1789,1793..1795,1799..1804,1808..1810,1841..1843)
Misc Feature(35)order(1460..1462,1667..1669,1673..1675,1787..1789,1793..1795,1799..1801,1850..1852,1904..1915)
Misc Feature(36)order(1484..1486,1490..1495,1574..1594,1604..1612,1745..1747,1754..1759,1766..1771,1817..1819,1823..1825,2204..2206)
Misc Feature(37)order(1838..1879,1895..1915)
Misc Feature(38)1856..1858
Misc Feature(39)1865..1867
Misc Feature(40)2072..2074
Misc Feature(41)2246..2248
Misc Feature(42)2246..2248
Misc Feature(43)2309..2311
Misc Feature(44)2309..2311
Misc Feature(45)2318..2320
Exon (1)1..199
Exon (2)200..301
Exon (3)302..565
Exon (4)566..669
Exon (5)670..772
Exon (6)773..921
Exon (7)922..1041
Exon (8)1042..1201
Exon (9)1202..1238
Exon (10)1239..1375
Exon (11)1376..1493
Exon (12)1494..1578
Exon (13)1579..1755
Exon (14)1756..1802
Exon (15)1803..1921
Exon (16)1922..2053
Exon (17)2054..2188
Exon (18)2189..2947
Translation

Target ORF information:

RefSeq Version NM_004333.4
Organism Homo sapiens(Human)
Definition Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_004333.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC
CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT
ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG
GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG
GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT
ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT
CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC
CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT
CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT
CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA
GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA
ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC
TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT
GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA
CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA
CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT
CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA
GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT
GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGGAT CAACCACAGG TTTGTCTGCT
ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA
GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA
CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA
CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG
GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT
GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC
ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT
CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT
GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT
AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG
AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG
GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT
GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC
AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG
GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA
AGAGATGAGA GACCACTCTT TCCCCAAATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA
TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG TTTCCAAACA
GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATCCAGGC AGGGGGATAT
GGTGCGTTTC CTGTCCACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu41737
Clone ID Related Accession (Same CDS sequence) XM_005250045.2
Accession Version XM_005250045.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2304bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product serine/threonine-protein kinase B-raf isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005250045.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC
CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT
ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG
GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG
GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT
ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT
CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC
CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT
CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT
CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA
GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA
ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC
TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT
GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA
CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA
CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT
CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA
GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT
GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGGAT CAACCACAGG TTTGTCTGCT
ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA
GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA
CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA
CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG
GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT
GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC
ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT
CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT
GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT
AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG
AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG
GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT
GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC
AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG
GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA
AGAGATGAGA GACCACTCTT TCCCCAAATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA
TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG TTTCCAAACA
GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATCCAGGC AGGGGGATAT
GGAGAATTTG CAGCCTTCAA GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005250102.1
CDS9..2312
Misc Feature(1)474..689
Misc Feature(2)order(480..482,495..497,501..512,516..518,555..557,567..575)
Misc Feature(3)711..848
Misc Feature(4)order(711..713,750..752,759..761,789..791,798..800,813..815,822..824,846..848)
Misc Feature(5)order(741..743,747..749)
Misc Feature(6)1380..2147
Misc Feature(7)1395..2153
Misc Feature(8)order(1395..1409,1419..1421,1449..1451,1455..1457,1548..1550,1593..1604,1614..1616,1620..1622,1734..1736,1740..1742,1746..1751,1755..1757,1788..1790,1797..1799,1851..1862)
Misc Feature(9)order(1395..1409,1419..1421,1449..1451,1455..1457,1548..1550,1593..1604,1614..1616,1734..1736,1740..1742,1746..1751,1755..1757,1788..1790)
Misc Feature(10)order(1407..1409,1614..1616,1620..1622,1734..1736,1740..1742,1746..1748,1797..1799,1851..1862)
Misc Feature(11)order(1431..1433,1437..1442,1521..1541,1551..1559,1692..1694,1701..1706,1713..1718,1764..1766,1770..1772,2151..2153)
Misc Feature(12)order(1785..1826,1842..1862)
Translation

Target ORF information:

RefSeq Version XM_005250045.2
Organism Homo sapiens(Human)
Definition Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005250045.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC
CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT
ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG
GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG
GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT
ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT
CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC
CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT
CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT
CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA
GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA
ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC
TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT
GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA
CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA
CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT
CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA
GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT
GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGGAT CAACCACAGG TTTGTCTGCT
ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA
GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA
CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA
CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG
GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT
GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC
ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT
CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT
GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT
AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG
AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG
GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT
GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC
AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG
GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA
AGAGATGAGA GACCACTCTT TCCCCAAATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA
TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG TTTCCAAACA
GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATCCAGGC AGGGGGATAT
GGAGAATTTG CAGCCTTCAA GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu97466
Clone ID Related Accession (Same CDS sequence) XM_017012558.1
Accession Version XM_017012558.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2424bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product serine/threonine-protein kinase B-raf isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC
CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT
ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG
GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG
GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT
ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT
CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC
CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT
CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT
CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA
GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA
ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC
TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT
GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA
CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA
CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT
CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA
GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT
GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGCCC CTTTGAACCA GCTGATGCGC
TGTCTTCGGA AATACCAATC CCGGACTCCC AGTCCCCTCC TACATTCTGT CCCCAGTGAA
ATAGTGTTTG ATTTTGAGCC TGGCCCAGTG TTCAGAGGAT CAACCACAGG TTTGTCTGCT
ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA
GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA
CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA
CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG
GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT
GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC
ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT
CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT
GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT
AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG
AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG
GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT
GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC
AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG
GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA
AGAGATGAGA GACCACTCTT TCCCCAAATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA
TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG TTTCCAAACA
GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATCCAGGC AGGGGGATAT
GGAGAATTTG CAGCCTTCAA GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016868047.1
CDS9..2432
Translation

Target ORF information:

RefSeq Version XM_017012558.1
Organism Homo sapiens(Human)
Definition Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017012558.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC
CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT
ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG
GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG
GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT
ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT
CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC
CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT
CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT
CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA
GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA
ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC
TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT
GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA
CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA
CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT
CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA
GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT
GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGCCC CTTTGAACCA GCTGATGCGC
TGTCTTCGGA AATACCAATC CCGGACTCCC AGTCCCCTCC TACATTCTGT CCCCAGTGAA
ATAGTGTTTG ATTTTGAGCC TGGCCCAGTG TTCAGAGGAT CAACCACAGG TTTGTCTGCT
ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA
GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA
CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA
CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG
GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT
GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC
ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT
CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT
GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT
AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG
AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG
GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT
GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC
AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG
GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA
AGAGATGAGA GACCACTCTT TCCCCAAATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA
TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG TTTCCAAACA
GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATCCAGGC AGGGGGATAT
GGAGAATTTG CAGCCTTCAA GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu97467
Clone ID Related Accession (Same CDS sequence) XM_017012559.1
Accession Version XM_017012559.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2397bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product serine/threonine-protein kinase B-raf isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC
CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT
ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG
GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG
GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT
ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT
CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC
CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT
CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT
CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA
GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA
ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC
TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT
GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA
CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA
CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT
CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA
GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT
GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGCCC CTTTGAACCA GCTGATGCGC
TGTCTTCGGA AATACCAATC CCGGACTCCC AGTCCCCTCC TACATTCTGT CCCCAGTGAA
ATAGTGTTTG ATTTTGAGCC TGGCCCAGTG TTCAGAGGAT CAACCACAGG TTTGTCTGCT
ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA
GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA
CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA
CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG
GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT
GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC
ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT
CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT
GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT
AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG
AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG
GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT
GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC
AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG
GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA
AGAGATGAGA GACCACTCTT TCCCCAAGAG AATTTGCAGC CTTCAAGTAG CCACCATCAT
GGCAGCATCT GCTCTTATTT CTTAAGTCTT GTGTTCGTAC AATTTGTTAA CATCAAAACA
CAGTTCTGTT CCTCAAATCT TTTTTTAAAG ATACAAAATT TCCAATGCAT AAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016868048.1
CDS9..2405
Translation

Target ORF information:

RefSeq Version XM_017012559.1
Organism Homo sapiens(Human)
Definition Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017012559.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 
GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC
CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT
ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG
GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG
GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT
ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT
CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC
CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT
CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT
CAGGATGGAG AGAAGAAACC AATTGGTTGG GACACTGATA TTTCCTGGCT TACTGGAGAA
GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA
ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC
TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT
GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA
CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA
CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT
CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA
GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT
GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGCCC CTTTGAACCA GCTGATGCGC
TGTCTTCGGA AATACCAATC CCGGACTCCC AGTCCCCTCC TACATTCTGT CCCCAGTGAA
ATAGTGTTTG ATTTTGAGCC TGGCCCAGTG TTCAGAGGAT CAACCACAGG TTTGTCTGCT
ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA
GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA
CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA
CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG
GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT
GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC
ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT
CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT
GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT
AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG
AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG
GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT
GCATTTGGAA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC
AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG
GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA
AGAGATGAGA GACCACTCTT TCCCCAAGAG AATTTGCAGC CTTCAAGTAG CCACCATCAT
GGCAGCATCT GCTCTTATTT CTTAAGTCTT GTGTTCGTAC AATTTGTTAA CATCAAAACA
CAGTTCTGTT CCTCAAATCT TTTTTTAAAG ATACAAAATT TCCAATGCAT AAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

A meta-analysis of the association between BRAF mutation and nonsmall cell lung cancer.
Medicine96(14)e6552(2017 Apr)
Cui G,Liu D,Li W,Fu X,Liang Y,Li Y,Shi W,Chen X,Zhao S


book

BRaf signaling principles unveiled by large-scale human mutation analysis with a rapid lentivirus-based gene replacement method.
Genes & development31(6)537-552(2017 Mar)
Lim CS,Kang X,Mirabella V,Zhang H,Bu Q,Araki Y,Hoang ET,Wang S,Shen Y,Choi S,Kaang BK,Chang Q,Pang ZP,Huganir RL,Zhu JJ


book

Evaluation and identification of factors related to
Journal of cancer research and therapeutics12(Supplement)C191-C198(2016 Dec)
Lin L,Chen GY,Xu CW,Wang HY,Wu YF,Fang MY


 
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