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ABCC8 cDNA ORF clone, Homo sapiens (human)

Gene Symbol ABCC8
Entrez Gene ID 6833
Full Name ATP-binding cassette, sub-family C (CFTR/MRP), member 8
Synonyms ABC36, HHF1, HI, HRINS, MRP8, PHHI, SUR, SUR1, SUR1delta2, TNDM2
General protein information
Preferred Names
ATP-binding cassette sub-family C member 8
Names
ATP-binding cassette sub-family C member 8
sulfonylurea receptor 1
sulfonylurea receptor (hyperinsulinemia)
ATP-binding cassette transporter sub-family C member 8
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11p15.1

Summary The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a modulator of ATP-sensitive potassium channels and insulin release. Mutations and deficiencies in this protein have been observed in patients with hyperinsulinemic hypoglycemia of infancy, an autosomal recessive disorder of unregulated and high insulin secretion. Mutations have also been associated with non-insulin-dependent diabetes mellitus type II, an autosomal dominant disease of defective insulin secretion. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]. lac of sum
Disorder MIM:

600509

Disorder Html: Hyperinsulinemic hypoglycemia, familial, 1, 256450 (3);

mRNA and Protein(s)

mRNA Protein Name
XM_011520331 XP_011518633 ATP-binding cassette sub-family C member 8 isoform X1
XM_011520332 XP_011518634 ATP-binding cassette sub-family C member 8 isoform X2
XM_011520333 XP_011518635 ATP-binding cassette sub-family C member 8 isoform X3
XM_011520334 XP_011518636 ATP-binding cassette sub-family C member 8 isoform X4
NM_000352 NP_000343 ATP-binding cassette sub-family C member 8 isoform 2
NM_001287174 NP_001274103 ATP-binding cassette sub-family C member 8 isoform 1



Homo sapiens (human) ABCC8 NP_001274103.1
Pan troglodytes (chimpanzee) ABCC8 XP_508310.4
Macaca mulatta (Rhesus monkey) ABCC8 XP_001088694.1
Canis lupus familiaris (dog) ABCC8 XP_005633781.1
Bos taurus (cattle) ABCC8 NP_001192539.1
Mus musculus (house mouse) Abcc8 NP_035640.2
Rattus norvegicus (Norway rat) Abcc8 NP_037171.2
Gallus gallus (chicken) ABCC8 XP_421005.2
Danio rerio (zebrafish) abcc8 NP_001166118.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ABCC8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ABCC8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu60816 XM_011520331 PREDICTED: Homo sapiens ATP-binding cassette, sub-family C (CFTR/MRP), member 8 (ABCC8), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $811.30
$1159.00
OHu60817 XM_011520332 PREDICTED: Homo sapiens ATP-binding cassette, sub-family C (CFTR/MRP), member 8 (ABCC8), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu60818 XM_011520333 PREDICTED: Homo sapiens ATP-binding cassette, sub-family C (CFTR/MRP), member 8 (ABCC8), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu60819 XM_011520334 PREDICTED: Homo sapiens ATP-binding cassette, sub-family C (CFTR/MRP), member 8 (ABCC8), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu21635 NM_000352 Homo sapiens ATP-binding cassette, sub-family C (CFTR/MRP), member 8 (ABCC8), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $811.30
$1159.00
OHu20799 NM_001287174 Homo sapiens ATP-binding cassette, sub-family C (CFTR/MRP), member 8 (ABCC8), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $811.30
$1159.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu60816
Clone ID Related Accession (Same CDS sequence) XM_011520331
Accession Version XM_011520331.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4746bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ATP-binding cassette sub-family C member 8 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009237.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
ATGCCCCTGG CCTTCTGCGG CAGCGAGAAC CACTCGGCCG CCTACCGGGT GGACCAGGGG 
GTCCTCAACA ACGGCTGCTT TGTGGACGCG CTCAACGTGG TGCCGCACGT CTTCCTACTC
TTCATCACCT TCCCCATCCT CTTCATTGGA TGGGGAAGTC AGAGCTCCAA GGTGCACATC
CACCACAGCA CATGGCTTCA TTTCCCTGGG CACAACCTGC GGTGGATCCT GACCTTCATG
CTGCTCTTCG TCCTGGTGTG TGAGATTGCA GAGGGCATCC TGTCTGATGG GGTGACCGAA
TCCCACCATC TGCACCTGTA CATGCCAGCC GGGATGGCGT TCATGGCTGC TGTCACCTCC
GTGGTCTACT ATCACAACAT CGAGACTTCC AACTTCCCCA AGCTGCTAAT TGCCCTGCTG
GTGTATTGGA CCCTGGCCTT CATCACCAAG ACCATCAAGT TTGTCAAGTT CTTGGACCAC
GCCATCGGCT TCTCGCAGCT ACGCTTCTGC CTCACAGGGC TGCTGGTGAT CCTCTATGGG
ATGCTGCTCC TCGTGGAGGT CAATGTCATC AGGGTGAGGA GATACATCTT CTTCAAGACA
CCGAGGGAGG TGAAGCCTCC CGAGGACCTG CAAGACCTGG GGGTACGCTT CCTGCAGCCC
TTCGTGAATC TGCTGTCCAA AGGCACCTAC TGGTGGATGA ACGCCTTCAT CAAGACTGCC
CACAAGAAGC CCATCGACTT GCGAGCCATC GGGAAGCTGC CCATCGCCAT GAGGGCCCTC
ACCAACTACC AACGGCTCTG CGAGGCCTTT GACGCCCAGC GGAAGGACAT TCAGGGCACT
CAAGGTGCCC GGGCCATCTG GCAGGCACTC AGCCATGCCT TCGGGAGGCG CCTGGTCCTC
AGCAGCACTT TCCGCATCTT GGCCGACCTG CTGGGCTTCG CCGGGCCACT GTGCATCTTT
GGGATCGTGG ACCACCTTGG GAAGGAGAAC GACGTCTTCC AGCCCAAGAC ACAATTTCTC
GGGGTTTACT TTGTCTCATC CCAAGAGTTC CTTGCCAATG CCTACGTCTT AGCTGTGCTT
CTGTTCCTTG CCCTCCTACT GCAAAGGACA TTTCTGCAAG CATCCTACTA TGTGGCCATT
GAAACTGGAA TTAACTTGAG AGGAGCAATA CAGACCAAGA TTTACAATAA AATTATGCAC
CTGTCCACCT CCAACCTGTC CATGGGAGAA ATGACTGCTG GACAGATCTG TAATCTGGTT
GCCATCGACA CCAATCAGCT CATGTGGTTT TTCTTCTTGT GCCCAAACCT CTGGGCTATG
CCAGTACAGA TCATTGTGGG TGTGATTCTC CTCTACTACA TACTCGGAGT CAGTGCCTTA
ATTGGAGCAG CTGTCATCAT TCTACTGGCT CCTGTCCAGT ACTTCGTGGC CACCAAGCTG
TCTCAGGCCC AGCGGAGCAC ACTGGAGTAT TCCAATGAGC GGCTGAAGCA GACCAACGAG
ATGCTCCGCG GCATCAAGCT GCTGAAGCTG TACGCCTGGG AGAACATCTT CCGCACGCGG
GTGGAGACGA CCCGCAGGAA GGAGATGACC AGCCTCAGGG CCTTTGCCAT CTATACCTCC
ATCTCCATTT TCATGAACAC GGCCATCCCC ATTGCAGCTG TCCTCATAAC TTTCGTGGGC
CACGTCAGCT TCTTCAAAGA GGCCGACTTC TCGCCCTCCG TGGCCTTTGC CTCCCTCTCC
CTCTTCCATA TCTTGGTCAC ACCGCTGTTC CTGCTGTCCA GTGTGGTCCG ATCTACCGTC
AAAGCTCTAG TGAGCGTGCA AAAGCTAAGC GAGTTCCTGT CCAGTGCAGA GATCCGTGAG
GAGCAGTGTG CCCCCCATGA GCCCACACCT CAGGGCCCAG CCAGCAAGTA CCAGGCGGTG
CCCCTCAGGG TTGTGAACCG CAAGCGTCCA GCCCGGGAGG ATTGTCGGGG CCTCACCGGC
CCACTGCAGA GCCTGGTCCC CAGTGCAGAT GGCGATGCTG ACAACTGCTG TGTCCAGATC
ATGGGAGGCT ACTTCACGTG GACCCCAGAT GGAATCCCCA CACTGTCCAA CATCACCATT
CGTATCCCCC GAGGCCAGCT GACTATGATC GTGGGGCAGG TGGGCTGCGG CAAGTCCTCG
CTCCTTCTAG CCGCACTGGG GGAGATGCAG AAGGTCTCAG GGGCTGTCTT CTGGAGCAGC
AGCCTTCCTG ACAGCGAGAT AGGAGAGGAC CCCAGCCCAG AGCGGGAGAC AGCGACCGAC
TTGGATATCA GGAAGAGAGG CCCCGTGGCC TATGCTTCGC AGAAACCATG GCTGCTAAAT
GCCACTGTGG AGGAGAACAT CATCTTTGAG AGTCCCTTCA ACAAACAACG GTACAAGATG
GTCATTGAAG CCTGCTCTCT GCAGCCAGAC ATCGACATCC TGCCCCATGG AGACCAGACC
CAGATTGGGG AACGGGGCAT CAACCTGTCT GGTGGTCAAC GCCAGCGAAT CAGTGTGGCC
CGAGCCCTCT ACCAGCACGC CAACGTTGTC TTCTTGGATG ACCCCTTCTC AGCTCTGGAT
ATCCATCTGA GTGACCACTT AATGCAGGCC GGCATCCTTG AGCTGCTCCG GGACGACAAG
AGGACAGTGG TCTTAGTGAC CCACAAGCTA CAGTACCTGC CCCATGCAGA CTGGATCATT
GCCATGAAGG ATGGCACCAT CCAGAGGGAG GGTACCCTCA AGGACTTCCA GAGGTCTGAA
TGCCAGCTCT TTGAGCACTG GAAGACCCTC ATGAACCGAC AGGACCAAGA GCTGGAGAAG
GAGACTGTCA CAGAGAGAAA AGCCACAGAG CCACCCCAGG GCCTATCTCG TGCCATGTCC
TCGAGGGATG GCCTTCTGCA GGATGAGGAA GAGGAGGAAG AGGAGGCAGC TGAGAGCGAG
GAGGATGACA ACCTGTCGTC CATGCTGCAC CAGCGTGCTG AGATCCCATG GCGAGCCTGC
GCCAAGTACC TGTCCTCCGC CGGCATCCTG CTCCTGTCGT TGCTGGTCTT CTCACAGCTG
CTCAAGCACA TGGTCCTGGT GGCCATCGAC TACTGGCTGG CCAAGTGGAC CGACAGCGCC
CTGACCCTGA CCCCTGCAGC CAGGAACTGC TCCCTCAGCC AGGAGTGCAC CCTCGACCAG
ACTGTCTATG CCATGGTGTT CACGGTGCTC TGCAGCCTGG GCATTGTGCT GTGCCTCGTC
ACGTCTGTCA CTGTGGAGTG GACAGGGCTG AAGGTGGCCA AGAGACTGCA CCGCAGCCTG
CTAAACCGGA TCATCCTAGC CCCCATGAGG TTTTTTGAGA CCACGCCCCT TGGGAGCATC
CTGAACAGAT TTTCATCTGA CTGTAACACC ATCGACCAGC ACATCCCATC CACGCTGGAG
TGCCTGAGCC GCTCCACCCT GCTCTGTGTC TCAGCCCTGG CCGTCATCTC CTATGTCACA
CCTGTGTTCC TCGTGGCCCT CTTGCCCCTG GCCATCGTGT GCTACTTCAT CCAGAAGTAC
TTCCGGGTGG CGTCCAGGGA CCTGCAGCAG CTGGATGACA CCACCCAGCT TCCACTTCTC
TCACACTTTG CCGAAACCGT AGAAGGACTC ACCACCATCC GGGCCTTCAG GTATGAGGCC
CGGTTCCAGC AGAAGCTTCT CGAATACACA GACTCCAACA ACATTGCTTC CCTCTTCCTC
ACAGCTGCCA ACAGATGGCT GGAAGTCCGA ATGGAGTACA TCGGTGCATG TGTGGTGCTC
ATCGCAGCGG TGACCTCCAT CTCCAACTCC CTGCACAGGG AGCTCTCTGC TGGCCTGGTG
GGCCTGGGCC TTACCTACGC CCTAATGGTC TCCAACTACC TCAACTGGAT GGTGAGGAAC
CTGGCAGACA TGGAGCTCCA GCTGGGGGCT GTGAAGCGCA TCCATGGGCT CCTGAAAACC
GAGGCAGAGA GCTACGAGGG GCTCCTGGCA CCATCGCTGA TCCCAAAGAA CTGGCCAGAC
CAAGGGAAGA TCCAGATCCA GAACCTGAGC GTGCGCTACG ACAGCTCCCT GAAGCCGGTG
CTGAAGCACG TCAATGCCCT CATCGCCCCT GGACAGAAGA TCGGGATCTG CGGCCGCACC
GGCAGTGGGA AGTCCTCCTT CTCTCTTGCC TTCTTCCGCA TGGTGGACAC GTTCGAAGGG
CACATCATCA TTGATGGCAT TGACATCGCC AAACTGCCGC TGCACACCCT GCGCTCACGC
CTCTCCATCA TCCTGCAGGA CCCCGTCCTC TTCAGCGGCA CCATCCGATT TAACCTGGAC
CCTGAGAGGA AGTGCTCAGA TAGCACACTG TGGGAGGCCC TGGAAATCGC CCAGCTGAAG
CTGGTGGTGA AGGCACTGCC AGGAGGCCTC GATGCCATCA TCACAGAAGG CGGGGAGAAT
TTCAGCCAGG GACAGAGGCA GCTGTTCTGC CTGGCCCGGG CCTTCGTGAG GAAGACCAGC
ATCTTCATCA TGGACGAGGC CACGGCTTCC ATTGACATGG CCACGGAAAA CATCCTCCAA
AAGGTGGTGA TGACAGCCTT CGCAGACCGC ACTGTGGTCA CCATCGCGCA TCGAGTGCAC
ACCATCCTGA GTGCAGACCT GGTGATCGTC CTGAAGCGGG GTGCCATCCT TGAGTTCGAT
AAGCCAGAGA AGCTGCTCAG CCGGAAGGAC AGCGTCTTCG CCTCCTTCGT CCGTGCAGAC
AAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011518633.1
CDS64..4809
Misc Feature(1)730..4806(+)
Misc Feature(2)1012..1830(+)
Misc Feature(3)2095..2781(+)
Misc Feature(4)2197..2220(+)
Misc Feature(5)2206..2727(+)
Misc Feature(6)2374..2385(+)
Misc Feature(7)2548..2577(+)
Misc Feature(8)2608..2625(+)
Misc Feature(9)2632..2643(+)
Misc Feature(10)2713..2733(+)
Misc Feature(11)3097..3945(+)
Misc Feature(12)4087..4806(+)
Misc Feature(13)4195..4218(+)
Misc Feature(14)4204..4683(+)
Misc Feature(15)4330..4341(+)
Misc Feature(16)4504..4533(+)
Misc Feature(17)4564..4581(+)
Misc Feature(18)4588..4599(+)
Misc Feature(19)4669..4689(+)
Translation

Target ORF information:

RefSeq Version XM_011520331
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens ATP-binding cassette, sub-family C (CFTR/MRP), member 8 (ABCC8), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011520331

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
ATGCCCCTGG CCTTCTGCGG CAGCGAGAAC CACTCGGCCG CCTACCGGGT GGACCAGGGG 
GTCCTCAACA ACGGCTGCTT TGTGGACGCG CTCAACGTGG TGCCGCACGT CTTCCTACTC
TTCATCACCT TCCCCATCCT CTTCATTGGA TGGGGAAGTC AGAGCTCCAA GGTGCACATC
CACCACAGCA CATGGCTTCA TTTCCCTGGG CACAACCTGC GGTGGATCCT GACCTTCATG
CTGCTCTTCG TCCTGGTGTG TGAGATTGCA GAGGGCATCC TGTCTGATGG GGTGACCGAA
TCCCACCATC TGCACCTGTA CATGCCAGCC GGGATGGCGT TCATGGCTGC TGTCACCTCC
GTGGTCTACT ATCACAACAT CGAGACTTCC AACTTCCCCA AGCTGCTAAT TGCCCTGCTG
GTGTATTGGA CCCTGGCCTT CATCACCAAG ACCATCAAGT TTGTCAAGTT CTTGGACCAC
GCCATCGGCT TCTCGCAGCT ACGCTTCTGC CTCACAGGGC TGCTGGTGAT CCTCTATGGG
ATGCTGCTCC TCGTGGAGGT CAATGTCATC AGGGTGAGGA GATACATCTT CTTCAAGACA
CCGAGGGAGG TGAAGCCTCC CGAGGACCTG CAAGACCTGG GGGTACGCTT CCTGCAGCCC
TTCGTGAATC TGCTGTCCAA AGGCACCTAC TGGTGGATGA ACGCCTTCAT CAAGACTGCC
CACAAGAAGC CCATCGACTT GCGAGCCATC GGGAAGCTGC CCATCGCCAT GAGGGCCCTC
ACCAACTACC AACGGCTCTG CGAGGCCTTT GACGCCCAGC GGAAGGACAT TCAGGGCACT
CAAGGTGCCC GGGCCATCTG GCAGGCACTC AGCCATGCCT TCGGGAGGCG CCTGGTCCTC
AGCAGCACTT TCCGCATCTT GGCCGACCTG CTGGGCTTCG CCGGGCCACT GTGCATCTTT
GGGATCGTGG ACCACCTTGG GAAGGAGAAC GACGTCTTCC AGCCCAAGAC ACAATTTCTC
GGGGTTTACT TTGTCTCATC CCAAGAGTTC CTTGCCAATG CCTACGTCTT AGCTGTGCTT
CTGTTCCTTG CCCTCCTACT GCAAAGGACA TTTCTGCAAG CATCCTACTA TGTGGCCATT
GAAACTGGAA TTAACTTGAG AGGAGCAATA CAGACCAAGA TTTACAATAA AATTATGCAC
CTGTCCACCT CCAACCTGTC CATGGGAGAA ATGACTGCTG GACAGATCTG TAATCTGGTT
GCCATCGACA CCAATCAGCT CATGTGGTTT TTCTTCTTGT GCCCAAACCT CTGGGCTATG
CCAGTACAGA TCATTGTGGG TGTGATTCTC CTCTACTACA TACTCGGAGT CAGTGCCTTA
ATTGGAGCAG CTGTCATCAT TCTACTGGCT CCTGTCCAGT ACTTCGTGGC CACCAAGCTG
TCTCAGGCCC AGCGGAGCAC ACTGGAGTAT TCCAATGAGC GGCTGAAGCA GACCAACGAG
ATGCTCCGCG GCATCAAGCT GCTGAAGCTG TACGCCTGGG AGAACATCTT CCGCACGCGG
GTGGAGACGA CCCGCAGGAA GGAGATGACC AGCCTCAGGG CCTTTGCCAT CTATACCTCC
ATCTCCATTT TCATGAACAC GGCCATCCCC ATTGCAGCTG TCCTCATAAC TTTCGTGGGC
CACGTCAGCT TCTTCAAAGA GGCCGACTTC TCGCCCTCCG TGGCCTTTGC CTCCCTCTCC
CTCTTCCATA TCTTGGTCAC ACCGCTGTTC CTGCTGTCCA GTGTGGTCCG ATCTACCGTC
AAAGCTCTAG TGAGCGTGCA AAAGCTAAGC GAGTTCCTGT CCAGTGCAGA GATCCGTGAG
GAGCAGTGTG CCCCCCATGA GCCCACACCT CAGGGCCCAG CCAGCAAGTA CCAGGCGGTG
CCCCTCAGGG TTGTGAACCG CAAGCGTCCA GCCCGGGAGG ATTGTCGGGG CCTCACCGGC
CCACTGCAGA GCCTGGTCCC CAGTGCAGAT GGCGATGCTG ACAACTGCTG TGTCCAGATC
ATGGGAGGCT ACTTCACGTG GACCCCAGAT GGAATCCCCA CACTGTCCAA CATCACCATT
CGTATCCCCC GAGGCCAGCT GACTATGATC GTGGGGCAGG TGGGCTGCGG CAAGTCCTCG
CTCCTTCTAG CCGCACTGGG GGAGATGCAG AAGGTCTCAG GGGCTGTCTT CTGGAGCAGC
AGCCTTCCTG ACAGCGAGAT AGGAGAGGAC CCCAGCCCAG AGCGGGAGAC AGCGACCGAC
TTGGATATCA GGAAGAGAGG CCCCGTGGCC TATGCTTCGC AGAAACCATG GCTGCTAAAT
GCCACTGTGG AGGAGAACAT CATCTTTGAG AGTCCCTTCA ACAAACAACG GTACAAGATG
GTCATTGAAG CCTGCTCTCT GCAGCCAGAC ATCGACATCC TGCCCCATGG AGACCAGACC
CAGATTGGGG AACGGGGCAT CAACCTGTCT GGTGGTCAAC GCCAGCGAAT CAGTGTGGCC
CGAGCCCTCT ACCAGCACGC CAACGTTGTC TTCTTGGATG ACCCCTTCTC AGCTCTGGAT
ATCCATCTGA GTGACCACTT AATGCAGGCC GGCATCCTTG AGCTGCTCCG GGACGACAAG
AGGACAGTGG TCTTAGTGAC CCACAAGCTA CAGTACCTGC CCCATGCAGA CTGGATCATT
GCCATGAAGG ATGGCACCAT CCAGAGGGAG GGTACCCTCA AGGACTTCCA GAGGTCTGAA
TGCCAGCTCT TTGAGCACTG GAAGACCCTC ATGAACCGAC AGGACCAAGA GCTGGAGAAG
GAGACTGTCA CAGAGAGAAA AGCCACAGAG CCACCCCAGG GCCTATCTCG TGCCATGTCC
TCGAGGGATG GCCTTCTGCA GGATGAGGAA GAGGAGGAAG AGGAGGCAGC TGAGAGCGAG
GAGGATGACA ACCTGTCGTC CATGCTGCAC CAGCGTGCTG AGATCCCATG GCGAGCCTGC
GCCAAGTACC TGTCCTCCGC CGGCATCCTG CTCCTGTCGT TGCTGGTCTT CTCACAGCTG
CTCAAGCACA TGGTCCTGGT GGCCATCGAC TACTGGCTGG CCAAGTGGAC CGACAGCGCC
CTGACCCTGA CCCCTGCAGC CAGGAACTGC TCCCTCAGCC AGGAGTGCAC CCTCGACCAG
ACTGTCTATG CCATGGTGTT CACGGTGCTC TGCAGCCTGG GCATTGTGCT GTGCCTCGTC
ACGTCTGTCA CTGTGGAGTG GACAGGGCTG AAGGTGGCCA AGAGACTGCA CCGCAGCCTG
CTAAACCGGA TCATCCTAGC CCCCATGAGG TTTTTTGAGA CCACGCCCCT TGGGAGCATC
CTGAACAGAT TTTCATCTGA CTGTAACACC ATCGACCAGC ACATCCCATC CACGCTGGAG
TGCCTGAGCC GCTCCACCCT GCTCTGTGTC TCAGCCCTGG CCGTCATCTC CTATGTCACA
CCTGTGTTCC TCGTGGCCCT CTTGCCCCTG GCCATCGTGT GCTACTTCAT CCAGAAGTAC
TTCCGGGTGG CGTCCAGGGA CCTGCAGCAG CTGGATGACA CCACCCAGCT TCCACTTCTC
TCACACTTTG CCGAAACCGT AGAAGGACTC ACCACCATCC GGGCCTTCAG GTATGAGGCC
CGGTTCCAGC AGAAGCTTCT CGAATACACA GACTCCAACA ACATTGCTTC CCTCTTCCTC
ACAGCTGCCA ACAGATGGCT GGAAGTCCGA ATGGAGTACA TCGGTGCATG TGTGGTGCTC
ATCGCAGCGG TGACCTCCAT CTCCAACTCC CTGCACAGGG AGCTCTCTGC TGGCCTGGTG
GGCCTGGGCC TTACCTACGC CCTAATGGTC TCCAACTACC TCAACTGGAT GGTGAGGAAC
CTGGCAGACA TGGAGCTCCA GCTGGGGGCT GTGAAGCGCA TCCATGGGCT CCTGAAAACC
GAGGCAGAGA GCTACGAGGG GCTCCTGGCA CCATCGCTGA TCCCAAAGAA CTGGCCAGAC
CAAGGGAAGA TCCAGATCCA GAACCTGAGC GTGCGCTACG ACAGCTCCCT GAAGCCGGTG
CTGAAGCACG TCAATGCCCT CATCGCCCCT GGACAGAAGA TCGGGATCTG CGGCCGCACC
GGCAGTGGGA AGTCCTCCTT CTCTCTTGCC TTCTTCCGCA TGGTGGACAC GTTCGAAGGG
CACATCATCA TTGATGGCAT TGACATCGCC AAACTGCCGC TGCACACCCT GCGCTCACGC
CTCTCCATCA TCCTGCAGGA CCCCGTCCTC TTCAGCGGCA CCATCCGATT TAACCTGGAC
CCTGAGAGGA AGTGCTCAGA TAGCACACTG TGGGAGGCCC TGGAAATCGC CCAGCTGAAG
CTGGTGGTGA AGGCACTGCC AGGAGGCCTC GATGCCATCA TCACAGAAGG CGGGGAGAAT
TTCAGCCAGG GACAGAGGCA GCTGTTCTGC CTGGCCCGGG CCTTCGTGAG GAAGACCAGC
ATCTTCATCA TGGACGAGGC CACGGCTTCC ATTGACATGG CCACGGAAAA CATCCTCCAA
AAGGTGGTGA TGACAGCCTT CGCAGACCGC ACTGTGGTCA CCATCGCGCA TCGAGTGCAC
ACCATCCTGA GTGCAGACCT GGTGATCGTC CTGAAGCGGG GTGCCATCCT TGAGTTCGAT
AAGCCAGAGA AGCTGCTCAG CCGGAAGGAC AGCGTCTTCG CCTCCTTCGT CCGTGCAGAC
AAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60817
Clone ID Related Accession (Same CDS sequence) XM_011520332
Accession Version XM_011520332.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4434bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ATP-binding cassette sub-family C member 8 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009237.19) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
ATGCCCCTGG CCTTCTGCGG CAGCGAGAAC CACTCGGCCG CCTACCGGGT GGACCAGGGG 
GTCCTCAACA ACGGCTGCTT TGTGGACGCG CTCAACGTGG TGCCGCACGT CTTCCTACTC
TTCATCACCT TCCCCATCCT CTTCATTGGA TGGGGAAGTC AGAGCTCCAA GGTGCACATC
CACCACAGCA CATGGCTTCA TTTCCCTGGG CACAACCTGC GGTGGATCCT GACCTTCATG
CTGCTCTTCG TCCTGGTGTG TGAGATTGCA GAGGGCATCC TGTCTGATGG GGTGACCGAA
TCCCACCATC TGCACCTGTA CATGCCAGCC GGGATGGCGT TCATGGCTGC TGTCACCTCC
GTGGTCTACT ATCACAACAT CGAGACTTCC AACTTCCCCA AGCTGCTAAT TGCCCTGCTG
GTGTATTGGA CCCTGGCCTT CATCACCAAG ACCATCAAGT TTGTCAAGTT CTTGGACCAC
GCCATCGGCT TCTCGCAGCT ACGCTTCTGC CTCACAGGGC TGCTGGTGAT CCTCTATGGG
ATGCTGCTCC TCGTGGAGGT CAATGTCATC AGGGTGAGGA GATACATCTT CTTCAAGACA
CCGAGGGAGG TGAAGCCTCC CGAGGACCTG CAAGACCTGG GGGTACGCTT CCTGCAGCCC
TTCGTGAATC TGCTGTCCAA AGGCACCTAC TGGTGGATGA ACGCCTTCAT CAAGACTGCC
CACAAGAAGC CCATCGACTT GCGAGCCATC GGGAAGCTGC CCATCGCCAT GAGGGCCCTC
ACCAACTACC AACGGCTCTG CGAGGCCTTT GACGCCCAGG TGCGGAAGGA CATTCAGGGC
ACTCAAGGTG CCCGGGCCAT CTGGCAGGCA CTCAGCCATG CCTTCGGGAG GCGCCTGGTC
CTCAGCAGCA CTTTCCGCAT CTTGGCCGAC CTGCTGGGCT TCGCCGGGCC ACTGTGCATC
TTTGGGATCG TGGACCACCT TGGGAAGGAG AACGACGTCT TCCAGCCCAA GACACAATTT
CTCGGGGTTT ACTTTGTCTC ATCCCAAGAG TTCCTTGCCA ATGCCTACGT CTTAGCTGTG
CTTCTGTTCC TTGCCCTCCT ACTGCAAAGG ACATTTCTGC AAGCATCCTA CTATGTGGCC
ATTGAAACTG GAATTAACTT GAGAGGAGCA ATACAGACCA AGATTTACAA TAAAATTATG
CACCTGTCCA CCTCCAACCT GTCCATGGGA GAAATGACTG CTGGACAGAT CTGTAATCTG
GTTGCCATCG ACACCAATCA GCTCATGTGG TTTTTCTTCT TGTGCCCAAA CCTCTGGGCT
ATGCCAGTAC AGATCATTGT GGGTGTGATT CTCCTCTACT ACATACTCGG AGTCAGTGCC
TTAATTGGAG CAGCTGTCAT CATTCTACTG GCTCCTGTCC AGTACTTCGT GGCCACCAAG
CTGTCTCAGG CCCAGCGGAG CACACTGGAG TATTCCAATG AGCGGCTGAA GCAGACCAAC
GAGATGCTCC GCGGCATCAA GCTGCTGAAG CTGTACGCCT GGGAGAACAT CTTCCGCACG
CGGGTGGAGA CGACCCGCAG GAAGGAGATG ACCAGCCTCA GGGCCTTTGC CATCTATACC
TCCATCTCCA TTTTCATGAA CACGGCCATC CCCATTGCAG CTGTCCTCAT AACTTTCGTG
GGCCACGTCA GCTTCTTCAA AGAGGCCGAC TTCTCGCCCT CCGTGGCCTT TGCCTCCCTC
TCCCTCTTCC ATATCTTGGT CACACCGCTG TTCCTGCTGT CCAGTGTGGT CCGATCTACC
GTCAAAGCTC TAGTGAGCGT GCAAAAGCTA AGCGAGTTCC TGTCCAGTGC AGAGATCCGT
GAGGAGCAGT GTGCCCCCCA TGAGCCCACA CCTCAGGGCC CAGCCAGCAA GTACCAGGCG
GTGCCCCTCA GGGTTGTGAA CCGCAAGCGT CCAGCCCGGG AGGATTGTCG GGGCCTCACC
GGCCCACTGC AGAGCCTGGT CCCCAGTGCA GATGGCGATG CTGACAACTG CTGTGTCCAG
ATCATGGGAG GCTACTTCAC GTGGACCCCA GATGGAATCC CCACACTGTC CAACATCACC
ATTCGTATCC CCCGAGGCCA GCTGACTATG ATCGTGGGGC AGGTGGGCTG CGGCAAGTCC
TCGCTCCTTC TAGCCGCACT GGGGGAGATG CAGAAGGTCT CAGGGGCTGT CTTCTGGAGC
AGCAGCCTTC CTGACAGCGA GATAGGAGAG GACCCCAGCC CAGAGCGGGA GACAGCGACC
GACTTGGATA TCAGGAAGAG AGGCCCCGTG GCCTATGCTT CGCAGAAACC ATGGCTGCTA
AATGCCACTG TGGAGGAGAA CATCATCTTT GAGAGTCCCT TCAACAAACA ACGGTACAAG
ATGGTCATTG AAGCCTGCTC TCTGCAGCCA GACATCGACA TCCTGCCCCA TGGAGACCAG
ACCCAGATTG GGGAACGGGG CATCAACCTG TCTGGTGGTC AACGCCAGCG AATCAGTGTG
GCCCGAGCCC TCTACCAGCA CGCCAACGTT GTCTTCTTGG ATGACCCCTT CTCAGCTCTG
GATATCCATC TGAGTGACCA CTTAATGCAG GCCGGCATCC TTGAGCTGCT CCGGGACGAC
AAGAGGACAG TGGTCTTAGT GACCCACAAG CTACAGTACC TGCCCCATGC AGACTGGATC
ATTGCCATGA AGGATGGCAC CATCCAGAGG GAGGGTACCC TCAAGGACTT CCAGAGGTCT
GAATGCCAGC TCTTTGAGCA CTGGAAGACC CTCATGAACC GACAGGACCA AGAGCTGGAG
AAGGAGACTG TCACAGAGAG AAAAGCCACA GAGCCACCCC AGGGCCTATC TCGTGCCATG
TCCTCGAGGG ATGGCCTTCT GCAGGATGAG GAAGAGGAGG AAGAGGAGGC AGCTGAGAGC
GAGGAGGATG ACAACCTGTC GTCCATGCTG CACCAGCGTG CTGAGATCCC ATGGCGAGCC
TGCGCCAAGT ACCTGTCCTC CGCCGGCATC CTGCTCCTGT CGTTGCTGGT CTTCTCACAG
CTGCTCAAGC ACATGGTCCT GGTGGCCATC GACTACTGGC TGGCCAAGTG GACCGACAGC
GCCCTGACCC TGACCCCTGC AGCCAGGAAC TGCTCCCTCA GCCAGGAGTG CACCCTCGAC
CAGACTGTCT ATGCCATGGT GTTCACGGTG CTCTGCAGCC TGGGCATTGT GCTGTGCCTC
GTCACGTCTG TCACTGTGGA GTGGACAGGG CTGAAGGTGG CCAAGAGACT GCACCGCAGC
CTGCTAAACC GGATCATCCT AGCCCCCATG AGGTTTTTTG AGACCACGCC CCTTGGGAGC
ATCCTGAACA GATTTTCATC TGACTGTAAC ACCATCGACC AGCACATCCC ATCCACGCTG
GAGTGCCTGA GCCGCTCCAC CCTGCTCTGT GTCTCAGCCC TGGCCGTCAT CTCCTATGTC
ACACCTGTGT TCCTCGTGGC CCTCTTGCCC CTGGCCATCG TGTGCTACTT CATCCAGAAG
TACTTCCGGG TGGCGTCCAG GGACCTGCAG CAGCTGGATG ACACCACCCA GCTTCCACTT
CTCTCACACT TTGCCGAAAC CGTAGAAGGA CTCACCACCA TCCGGGCCTT CAGGTATGAG
GCCCGGTTCC AGCAGAAGCT TCTCGAATAC ACAGACTCCA ACAACATTGC TTCCCTCTTC
CTCACAGCTG CCAACAGATG GCTGGAAGTC CGAATGGAGT ACATCGGTGC ATGTGTGGTG
CTCATCGCAG CGGTGACCTC CATCTCCAAC TCCCTGCACA GGGAGCTCTC TGCTGGCCTG
GTGGGCCTGG GCCTTACCTA CGCCCTAATG GTCTCCAACT ACCTCAACTG GATGGTGAGG
AACCTGGCAG ACATGGAGCT CCAGCTGGGG GCTGTGAAGC GCATCCATGG GCTCCTGAAA
ACCGAGGCAG AGAGCTACGA GGGGCTCCTG GCACCATCGC TGATCCCAAA GAACTGGCCA
GACCAAGGGA AGATCCAGAT CCAGAACCTG AGCGTGCGCT ACGACAGCTC CCTGAAGCCG
GTGCTGAAGC ACGTCAATGC CCTCATCGCC CCTGGACAGA AGATCGGGAT CTGCGGCCGC
ACCGGCAGTG GGAAGTCCTC CTTCTCTCTT GCCTTCTTCC GCATGGTGGA CACGTTCGAA
GGGCACATCA TCATTGATGG CATTGACATC GCCAAACTGC CGCTGCACAC CCTGCGCTCA
CGCCTCTCCA TCATCCTGCA GGACCCCGTC CTCTTCAGCG GCACCATCCG ATGCCATCAT
CACAGAAGGC GGGGAGAATT TCAGCCAGGG ACAGAGGCAG CTGTTCTGCC TGGCCCGGGC
CTTCGTGAGG AAGACCAGCA TCTTCATCAT GGACGAGGCC ACGGCTTCCA TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011518634.1
CDS64..4497
Misc Feature(1)922..2862(+)
Misc Feature(2)1015..1833(+)
Misc Feature(3)2098..2784(+)
Misc Feature(4)2200..2223(+)
Misc Feature(5)2209..2730(+)
Misc Feature(6)2377..2388(+)
Misc Feature(7)2551..2580(+)
Misc Feature(8)2611..2628(+)
Misc Feature(9)2635..2646(+)
Misc Feature(10)2716..2736(+)
Misc Feature(11)3055..>4374(+)
Misc Feature(12)3100..3948(+)
Misc Feature(13)4090..>4374(+)
Misc Feature(14)4198..4221(+)
Misc Feature(15)4207..4344(+)
Misc Feature(16)4333..4344(+)
Translation

Target ORF information:

RefSeq Version XM_011520332
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens ATP-binding cassette, sub-family C (CFTR/MRP), member 8 (ABCC8), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011520332

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
ATGCCCCTGG CCTTCTGCGG CAGCGAGAAC CACTCGGCCG CCTACCGGGT GGACCAGGGG 
GTCCTCAACA ACGGCTGCTT TGTGGACGCG CTCAACGTGG TGCCGCACGT CTTCCTACTC
TTCATCACCT TCCCCATCCT CTTCATTGGA TGGGGAAGTC AGAGCTCCAA GGTGCACATC
CACCACAGCA CATGGCTTCA TTTCCCTGGG CACAACCTGC GGTGGATCCT GACCTTCATG
CTGCTCTTCG TCCTGGTGTG TGAGATTGCA GAGGGCATCC TGTCTGATGG GGTGACCGAA
TCCCACCATC TGCACCTGTA CATGCCAGCC GGGATGGCGT TCATGGCTGC TGTCACCTCC
GTGGTCTACT ATCACAACAT CGAGACTTCC AACTTCCCCA AGCTGCTAAT TGCCCTGCTG
GTGTATTGGA CCCTGGCCTT CATCACCAAG ACCATCAAGT TTGTCAAGTT CTTGGACCAC
GCCATCGGCT TCTCGCAGCT ACGCTTCTGC CTCACAGGGC TGCTGGTGAT CCTCTATGGG
ATGCTGCTCC TCGTGGAGGT CAATGTCATC AGGGTGAGGA GATACATCTT CTTCAAGACA
CCGAGGGAGG TGAAGCCTCC CGAGGACCTG CAAGACCTGG GGGTACGCTT CCTGCAGCCC
TTCGTGAATC TGCTGTCCAA AGGCACCTAC TGGTGGATGA ACGCCTTCAT CAAGACTGCC
CACAAGAAGC CCATCGACTT GCGAGCCATC GGGAAGCTGC CCATCGCCAT GAGGGCCCTC
ACCAACTACC AACGGCTCTG CGAGGCCTTT GACGCCCAGG TGCGGAAGGA CATTCAGGGC
ACTCAAGGTG CCCGGGCCAT CTGGCAGGCA CTCAGCCATG CCTTCGGGAG GCGCCTGGTC
CTCAGCAGCA CTTTCCGCAT CTTGGCCGAC CTGCTGGGCT TCGCCGGGCC ACTGTGCATC
TTTGGGATCG TGGACCACCT TGGGAAGGAG AACGACGTCT TCCAGCCCAA GACACAATTT
CTCGGGGTTT ACTTTGTCTC ATCCCAAGAG TTCCTTGCCA ATGCCTACGT CTTAGCTGTG
CTTCTGTTCC TTGCCCTCCT ACTGCAAAGG ACATTTCTGC AAGCATCCTA CTATGTGGCC
ATTGAAACTG GAATTAACTT GAGAGGAGCA ATACAGACCA AGATTTACAA TAAAATTATG
CACCTGTCCA CCTCCAACCT GTCCATGGGA GAAATGACTG CTGGACAGAT CTGTAATCTG
GTTGCCATCG ACACCAATCA GCTCATGTGG TTTTTCTTCT TGTGCCCAAA CCTCTGGGCT
ATGCCAGTAC AGATCATTGT GGGTGTGATT CTCCTCTACT ACATACTCGG AGTCAGTGCC
TTAATTGGAG CAGCTGTCAT CATTCTACTG GCTCCTGTCC AGTACTTCGT GGCCACCAAG
CTGTCTCAGG CCCAGCGGAG CACACTGGAG TATTCCAATG AGCGGCTGAA GCAGACCAAC
GAGATGCTCC GCGGCATCAA GCTGCTGAAG CTGTACGCCT GGGAGAACAT CTTCCGCACG
CGGGTGGAGA CGACCCGCAG GAAGGAGATG ACCAGCCTCA GGGCCTTTGC CATCTATACC
TCCATCTCCA TTTTCATGAA CACGGCCATC CCCATTGCAG CTGTCCTCAT AACTTTCGTG
GGCCACGTCA GCTTCTTCAA AGAGGCCGAC TTCTCGCCCT CCGTGGCCTT TGCCTCCCTC
TCCCTCTTCC ATATCTTGGT CACACCGCTG TTCCTGCTGT CCAGTGTGGT CCGATCTACC
GTCAAAGCTC TAGTGAGCGT GCAAAAGCTA AGCGAGTTCC TGTCCAGTGC AGAGATCCGT
GAGGAGCAGT GTGCCCCCCA TGAGCCCACA CCTCAGGGCC CAGCCAGCAA GTACCAGGCG
GTGCCCCTCA GGGTTGTGAA CCGCAAGCGT CCAGCCCGGG AGGATTGTCG GGGCCTCACC
GGCCCACTGC AGAGCCTGGT CCCCAGTGCA GATGGCGATG CTGACAACTG CTGTGTCCAG
ATCATGGGAG GCTACTTCAC GTGGACCCCA GATGGAATCC CCACACTGTC CAACATCACC
ATTCGTATCC CCCGAGGCCA GCTGACTATG ATCGTGGGGC AGGTGGGCTG CGGCAAGTCC
TCGCTCCTTC TAGCCGCACT GGGGGAGATG CAGAAGGTCT CAGGGGCTGT CTTCTGGAGC
AGCAGCCTTC CTGACAGCGA GATAGGAGAG GACCCCAGCC CAGAGCGGGA GACAGCGACC
GACTTGGATA TCAGGAAGAG AGGCCCCGTG GCCTATGCTT CGCAGAAACC ATGGCTGCTA
AATGCCACTG TGGAGGAGAA CATCATCTTT GAGAGTCCCT TCAACAAACA ACGGTACAAG
ATGGTCATTG AAGCCTGCTC TCTGCAGCCA GACATCGACA TCCTGCCCCA TGGAGACCAG
ACCCAGATTG GGGAACGGGG CATCAACCTG TCTGGTGGTC AACGCCAGCG AATCAGTGTG
GCCCGAGCCC TCTACCAGCA CGCCAACGTT GTCTTCTTGG ATGACCCCTT CTCAGCTCTG
GATATCCATC TGAGTGACCA CTTAATGCAG GCCGGCATCC TTGAGCTGCT CCGGGACGAC
AAGAGGACAG TGGTCTTAGT GACCCACAAG CTACAGTACC TGCCCCATGC AGACTGGATC
ATTGCCATGA AGGATGGCAC CATCCAGAGG GAGGGTACCC TCAAGGACTT CCAGAGGTCT
GAATGCCAGC TCTTTGAGCA CTGGAAGACC CTCATGAACC GACAGGACCA AGAGCTGGAG
AAGGAGACTG TCACAGAGAG AAAAGCCACA GAGCCACCCC AGGGCCTATC TCGTGCCATG
TCCTCGAGGG ATGGCCTTCT GCAGGATGAG GAAGAGGAGG AAGAGGAGGC AGCTGAGAGC
GAGGAGGATG ACAACCTGTC GTCCATGCTG CACCAGCGTG CTGAGATCCC ATGGCGAGCC
TGCGCCAAGT ACCTGTCCTC CGCCGGCATC CTGCTCCTGT CGTTGCTGGT CTTCTCACAG
CTGCTCAAGC ACATGGTCCT GGTGGCCATC GACTACTGGC TGGCCAAGTG GACCGACAGC
GCCCTGACCC TGACCCCTGC AGCCAGGAAC TGCTCCCTCA GCCAGGAGTG CACCCTCGAC
CAGACTGTCT ATGCCATGGT GTTCACGGTG CTCTGCAGCC TGGGCATTGT GCTGTGCCTC
GTCACGTCTG TCACTGTGGA GTGGACAGGG CTGAAGGTGG CCAAGAGACT GCACCGCAGC
CTGCTAAACC GGATCATCCT AGCCCCCATG AGGTTTTTTG AGACCACGCC CCTTGGGAGC
ATCCTGAACA GATTTTCATC TGACTGTAAC ACCATCGACC AGCACATCCC ATCCACGCTG
GAGTGCCTGA GCCGCTCCAC CCTGCTCTGT GTCTCAGCCC TGGCCGTCAT CTCCTATGTC
ACACCTGTGT TCCTCGTGGC CCTCTTGCCC CTGGCCATCG TGTGCTACTT CATCCAGAAG
TACTTCCGGG TGGCGTCCAG GGACCTGCAG CAGCTGGATG ACACCACCCA GCTTCCACTT
CTCTCACACT TTGCCGAAAC CGTAGAAGGA CTCACCACCA TCCGGGCCTT CAGGTATGAG
GCCCGGTTCC AGCAGAAGCT TCTCGAATAC ACAGACTCCA ACAACATTGC TTCCCTCTTC
CTCACAGCTG CCAACAGATG GCTGGAAGTC CGAATGGAGT ACATCGGTGC ATGTGTGGTG
CTCATCGCAG CGGTGACCTC CATCTCCAAC TCCCTGCACA GGGAGCTCTC TGCTGGCCTG
GTGGGCCTGG GCCTTACCTA CGCCCTAATG GTCTCCAACT ACCTCAACTG GATGGTGAGG
AACCTGGCAG ACATGGAGCT CCAGCTGGGG GCTGTGAAGC GCATCCATGG GCTCCTGAAA
ACCGAGGCAG AGAGCTACGA GGGGCTCCTG GCACCATCGC TGATCCCAAA GAACTGGCCA
GACCAAGGGA AGATCCAGAT CCAGAACCTG AGCGTGCGCT ACGACAGCTC CCTGAAGCCG
GTGCTGAAGC ACGTCAATGC CCTCATCGCC CCTGGACAGA AGATCGGGAT CTGCGGCCGC
ACCGGCAGTG GGAAGTCCTC CTTCTCTCTT GCCTTCTTCC GCATGGTGGA CACGTTCGAA
GGGCACATCA TCATTGATGG CATTGACATC GCCAAACTGC CGCTGCACAC CCTGCGCTCA
CGCCTCTCCA TCATCCTGCA GGACCCCGTC CTCTTCAGCG GCACCATCCG ATGCCATCAT
CACAGAAGGC GGGGAGAATT TCAGCCAGGG ACAGAGGCAG CTGTTCTGCC TGGCCCGGGC
CTTCGTGAGG AAGACCAGCA TCTTCATCAT GGACGAGGCC ACGGCTTCCA TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60818
Clone ID Related Accession (Same CDS sequence) XM_011520333
Accession Version XM_011520333.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3246bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ATP-binding cassette sub-family C member 8 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009237.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGCTCCGCG GCATCAAGCT GCTGAAGCTG TACGCCTGGG AGAACATCTT CCGCACGCGG 
GTGGAGACGA CCCGCAGGAA GGAGATGACC AGCCTCAGGG CCTTTGCCAT CTATACCTCC
ATCTCCATTT TCATGAACAC GGCCATCCCC ATTGCAGCTG TCCTCATAAC TTTCGTGGGC
CACGTCAGCT TCTTCAAAGA GGCCGACTTC TCGCCCTCCG TGGCCTTTGC CTCCCTCTCC
CTCTTCCATA TCTTGGTCAC ACCGCTGTTC CTGCTGTCCA GTGTGGTCCG ATCTACCGTC
AAAGCTCTAG TGAGCGTGCA AAAGCTAAGC GAGTTCCTGT CCAGTGCAGA GATCCGTGAG
GAGCAGTGTG CCCCCCATGA GCCCACACCT CAGGGCCCAG CCAGCAAGTA CCAGGCGGTG
CCCCTCAGGG TTGTGAACCG CAAGCGTCCA GCCCGGGAGG ATTGTCGGGG CCTCACCGGC
CCACTGCAGA GCCTGGTCCC CAGTGCAGAT GGCGATGCTG ACAACTGCTG TGTCCAGATC
ATGGGAGGCT ACTTCACGTG GACCCCAGAT GGAATCCCCA CACTGTCCAA CATCACCATT
CGTATCCCCC GAGGCCAGCT GACTATGATC GTGGGGCAGG TGGGCTGCGG CAAGTCCTCG
CTCCTTCTAG CCGCACTGGG GGAGATGCAG AAGGTCTCAG GGGCTGTCTT CTGGAGCAGC
AGCCTTCCTG ACAGCGAGAT AGGAGAGGAC CCCAGCCCAG AGCGGGAGAC AGCGACCGAC
TTGGATATCA GGAAGAGAGG CCCCGTGGCC TATGCTTCGC AGAAACCATG GCTGCTAAAT
GCCACTGTGG AGGAGAACAT CATCTTTGAG AGTCCCTTCA ACAAACAACG GTACAAGATG
GTCATTGAAG CCTGCTCTCT GCAGCCAGAC ATCGACATCC TGCCCCATGG AGACCAGACC
CAGATTGGGG AACGGGGCAT CAACCTGTCT GGTGGTCAAC GCCAGCGAAT CAGTGTGGCC
CGAGCCCTCT ACCAGCACGC CAACGTTGTC TTCTTGGATG ACCCCTTCTC AGCTCTGGAT
ATCCATCTGA GTGACCACTT AATGCAGGCC GGCATCCTTG AGCTGCTCCG GGACGACAAG
AGGACAGTGG TCTTAGTGAC CCACAAGCTA CAGTACCTGC CCCATGCAGA CTGGATCATT
GCCATGAAGG ATGGCACCAT CCAGAGGGAG GGTACCCTCA AGGACTTCCA GAGGTCTGAA
TGCCAGCTCT TTGAGCACTG GAAGACCCTC ATGAACCGAC AGGACCAAGA GCTGGAGAAG
GAGACTGTCA CAGAGAGAAA AGCCACAGAG CCACCCCAGG GCCTATCTCG TGCCATGTCC
TCGAGGGATG GCCTTCTGCA GGATGAGGAA GAGGAGGAAG AGGAGGCAGC TGAGAGCGAG
GAGGATGACA ACCTGTCGTC CATGCTGCAC CAGCGTGCTG AGATCCCATG GCGAGCCTGC
GCCAAGTACC TGTCCTCCGC CGGCATCCTG CTCCTGTCGT TGCTGGTCTT CTCACAGCTG
CTCAAGCACA TGGTCCTGGT GGCCATCGAC TACTGGCTGG CCAAGTGGAC CGACAGCGCC
CTGACCCTGA CCCCTGCAGC CAGGAACTGC TCCCTCAGCC AGGAGTGCAC CCTCGACCAG
ACTGTCTATG CCATGGTGTT CACGGTGCTC TGCAGCCTGG GCATTGTGCT GTGCCTCGTC
ACGTCTGTCA CTGTGGAGTG GACAGGGCTG AAGGTGGCCA AGAGACTGCA CCGCAGCCTG
CTAAACCGGA TCATCCTAGC CCCCATGAGG TTTTTTGAGA CCACGCCCCT TGGGAGCATC
CTGAACAGAT TTTCATCTGA CTGTAACACC ATCGACCAGC ACATCCCATC CACGCTGGAG
TGCCTGAGCC GCTCCACCCT GCTCTGTGTC TCAGCCCTGG CCGTCATCTC CTATGTCACA
CCTGTGTTCC TCGTGGCCCT CTTGCCCCTG GCCATCGTGT GCTACTTCAT CCAGAAGTAC
TTCCGGGTGG CGTCCAGGGA CCTGCAGCAG CTGGATGACA CCACCCAGCT TCCACTTCTC
TCACACTTTG CCGAAACCGT AGAAGGACTC ACCACCATCC GGGCCTTCAG GTATGAGGCC
CGGTTCCAGC AGAAGCTTCT CGAATACACA GACTCCAACA ACATTGCTTC CCTCTTCCTC
ACAGCTGCCA ACAGATGGCT GGAAGTCCGA ATGGAGTACA TCGGTGCATG TGTGGTGCTC
ATCGCAGCGG TGACCTCCAT CTCCAACTCC CTGCACAGGG AGCTCTCTGC TGGCCTGGTG
GGCCTGGGCC TTACCTACGC CCTAATGGTC TCCAACTACC TCAACTGGAT GGTGAGGAAC
CTGGCAGACA TGGAGCTCCA GCTGGGGGCT GTGAAGCGCA TCCATGGGCT CCTGAAAACC
GAGGCAGAGA GCTACGAGGG GCTCCTGGCA CCATCGCTGA TCCCAAAGAA CTGGCCAGAC
CAAGGGAAGA TCCAGATCCA GAACCTGAGC GTGCGCTACG ACAGCTCCCT GAAGCCGGTG
CTGAAGCACG TCAATGCCCT CATCGCCCCT GGACAGAAGA TCGGGATCTG CGGCCGCACC
GGCAGTGGGA AGTCCTCCTT CTCTCTTGCC TTCTTCCGCA TGGTGGACAC GTTCGAAGGG
CACATCATCA TTGATGGCAT TGACATCGCC AAACTGCCGC TGCACACCCT GCGCTCACGC
CTCTCCATCA TCCTGCAGGA CCCCGTCCTC TTCAGCGGCA CCATCCGATT TAACCTGGAC
CCTGAGAGGA AGTGCTCAGA TAGCACACTG TGGGAGGCCC TGGAAATCGC CCAGCTGAAG
CTGGTGGTGA AGGCACTGCC AGGAGGCCTC GATGCCATCA TCACAGAAGG CGGGGAGAAT
TTCAGCCAGG GACAGAGGCA GCTGTTCTGC CTGGCCCGGG CCTTCGTGAG GAAGACCAGC
ATCTTCATCA TGGACGAGGC CACGGCTTCC ATTGACATGG CCACGGAAAA CATCCTCCAA
AAGGTGGTGA TGACAGCCTT CGCAGACCGC ACTGTGGTCA CCATCGCGCA TCGAGTGCAC
ACCATCCTGA GTGCAGACCT GGTGATCGTC CTGAAGCGGG GTGCCATCCT TGAGTTCGAT
AAGCCAGAGA AGCTGCTCAG CCGGAAGGAC AGCGTCTTCG CCTCCTTCGT CCGTGCAGAC
AAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011518635.1
CDS123..3368
Misc Feature(1)123..389(+)
Misc Feature(2)654..1340(+)
Misc Feature(3)672..1370(+)
Misc Feature(4)756..779(+)
Misc Feature(5)765..1286(+)
Misc Feature(6)933..944(+)
Misc Feature(7)1107..1136(+)
Misc Feature(8)1167..1184(+)
Misc Feature(9)1191..1202(+)
Misc Feature(10)1272..1292(+)
Misc Feature(11)1611..3359(+)
Misc Feature(12)1656..2504(+)
Misc Feature(13)2646..3365(+)
Misc Feature(14)2754..2777(+)
Misc Feature(15)2763..3242(+)
Misc Feature(16)2889..2900(+)
Misc Feature(17)3063..3092(+)
Misc Feature(18)3123..3140(+)
Misc Feature(19)3147..3158(+)
Misc Feature(20)3228..3248(+)
Translation

Target ORF information:

RefSeq Version XM_011520333
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens ATP-binding cassette, sub-family C (CFTR/MRP), member 8 (ABCC8), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011520333

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGCTCCGCG GCATCAAGCT GCTGAAGCTG TACGCCTGGG AGAACATCTT CCGCACGCGG 
GTGGAGACGA CCCGCAGGAA GGAGATGACC AGCCTCAGGG CCTTTGCCAT CTATACCTCC
ATCTCCATTT TCATGAACAC GGCCATCCCC ATTGCAGCTG TCCTCATAAC TTTCGTGGGC
CACGTCAGCT TCTTCAAAGA GGCCGACTTC TCGCCCTCCG TGGCCTTTGC CTCCCTCTCC
CTCTTCCATA TCTTGGTCAC ACCGCTGTTC CTGCTGTCCA GTGTGGTCCG ATCTACCGTC
AAAGCTCTAG TGAGCGTGCA AAAGCTAAGC GAGTTCCTGT CCAGTGCAGA GATCCGTGAG
GAGCAGTGTG CCCCCCATGA GCCCACACCT CAGGGCCCAG CCAGCAAGTA CCAGGCGGTG
CCCCTCAGGG TTGTGAACCG CAAGCGTCCA GCCCGGGAGG ATTGTCGGGG CCTCACCGGC
CCACTGCAGA GCCTGGTCCC CAGTGCAGAT GGCGATGCTG ACAACTGCTG TGTCCAGATC
ATGGGAGGCT ACTTCACGTG GACCCCAGAT GGAATCCCCA CACTGTCCAA CATCACCATT
CGTATCCCCC GAGGCCAGCT GACTATGATC GTGGGGCAGG TGGGCTGCGG CAAGTCCTCG
CTCCTTCTAG CCGCACTGGG GGAGATGCAG AAGGTCTCAG GGGCTGTCTT CTGGAGCAGC
AGCCTTCCTG ACAGCGAGAT AGGAGAGGAC CCCAGCCCAG AGCGGGAGAC AGCGACCGAC
TTGGATATCA GGAAGAGAGG CCCCGTGGCC TATGCTTCGC AGAAACCATG GCTGCTAAAT
GCCACTGTGG AGGAGAACAT CATCTTTGAG AGTCCCTTCA ACAAACAACG GTACAAGATG
GTCATTGAAG CCTGCTCTCT GCAGCCAGAC ATCGACATCC TGCCCCATGG AGACCAGACC
CAGATTGGGG AACGGGGCAT CAACCTGTCT GGTGGTCAAC GCCAGCGAAT CAGTGTGGCC
CGAGCCCTCT ACCAGCACGC CAACGTTGTC TTCTTGGATG ACCCCTTCTC AGCTCTGGAT
ATCCATCTGA GTGACCACTT AATGCAGGCC GGCATCCTTG AGCTGCTCCG GGACGACAAG
AGGACAGTGG TCTTAGTGAC CCACAAGCTA CAGTACCTGC CCCATGCAGA CTGGATCATT
GCCATGAAGG ATGGCACCAT CCAGAGGGAG GGTACCCTCA AGGACTTCCA GAGGTCTGAA
TGCCAGCTCT TTGAGCACTG GAAGACCCTC ATGAACCGAC AGGACCAAGA GCTGGAGAAG
GAGACTGTCA CAGAGAGAAA AGCCACAGAG CCACCCCAGG GCCTATCTCG TGCCATGTCC
TCGAGGGATG GCCTTCTGCA GGATGAGGAA GAGGAGGAAG AGGAGGCAGC TGAGAGCGAG
GAGGATGACA ACCTGTCGTC CATGCTGCAC CAGCGTGCTG AGATCCCATG GCGAGCCTGC
GCCAAGTACC TGTCCTCCGC CGGCATCCTG CTCCTGTCGT TGCTGGTCTT CTCACAGCTG
CTCAAGCACA TGGTCCTGGT GGCCATCGAC TACTGGCTGG CCAAGTGGAC CGACAGCGCC
CTGACCCTGA CCCCTGCAGC CAGGAACTGC TCCCTCAGCC AGGAGTGCAC CCTCGACCAG
ACTGTCTATG CCATGGTGTT CACGGTGCTC TGCAGCCTGG GCATTGTGCT GTGCCTCGTC
ACGTCTGTCA CTGTGGAGTG GACAGGGCTG AAGGTGGCCA AGAGACTGCA CCGCAGCCTG
CTAAACCGGA TCATCCTAGC CCCCATGAGG TTTTTTGAGA CCACGCCCCT TGGGAGCATC
CTGAACAGAT TTTCATCTGA CTGTAACACC ATCGACCAGC ACATCCCATC CACGCTGGAG
TGCCTGAGCC GCTCCACCCT GCTCTGTGTC TCAGCCCTGG CCGTCATCTC CTATGTCACA
CCTGTGTTCC TCGTGGCCCT CTTGCCCCTG GCCATCGTGT GCTACTTCAT CCAGAAGTAC
TTCCGGGTGG CGTCCAGGGA CCTGCAGCAG CTGGATGACA CCACCCAGCT TCCACTTCTC
TCACACTTTG CCGAAACCGT AGAAGGACTC ACCACCATCC GGGCCTTCAG GTATGAGGCC
CGGTTCCAGC AGAAGCTTCT CGAATACACA GACTCCAACA ACATTGCTTC CCTCTTCCTC
ACAGCTGCCA ACAGATGGCT GGAAGTCCGA ATGGAGTACA TCGGTGCATG TGTGGTGCTC
ATCGCAGCGG TGACCTCCAT CTCCAACTCC CTGCACAGGG AGCTCTCTGC TGGCCTGGTG
GGCCTGGGCC TTACCTACGC CCTAATGGTC TCCAACTACC TCAACTGGAT GGTGAGGAAC
CTGGCAGACA TGGAGCTCCA GCTGGGGGCT GTGAAGCGCA TCCATGGGCT CCTGAAAACC
GAGGCAGAGA GCTACGAGGG GCTCCTGGCA CCATCGCTGA TCCCAAAGAA CTGGCCAGAC
CAAGGGAAGA TCCAGATCCA GAACCTGAGC GTGCGCTACG ACAGCTCCCT GAAGCCGGTG
CTGAAGCACG TCAATGCCCT CATCGCCCCT GGACAGAAGA TCGGGATCTG CGGCCGCACC
GGCAGTGGGA AGTCCTCCTT CTCTCTTGCC TTCTTCCGCA TGGTGGACAC GTTCGAAGGG
CACATCATCA TTGATGGCAT TGACATCGCC AAACTGCCGC TGCACACCCT GCGCTCACGC
CTCTCCATCA TCCTGCAGGA CCCCGTCCTC TTCAGCGGCA CCATCCGATT TAACCTGGAC
CCTGAGAGGA AGTGCTCAGA TAGCACACTG TGGGAGGCCC TGGAAATCGC CCAGCTGAAG
CTGGTGGTGA AGGCACTGCC AGGAGGCCTC GATGCCATCA TCACAGAAGG CGGGGAGAAT
TTCAGCCAGG GACAGAGGCA GCTGTTCTGC CTGGCCCGGG CCTTCGTGAG GAAGACCAGC
ATCTTCATCA TGGACGAGGC CACGGCTTCC ATTGACATGG CCACGGAAAA CATCCTCCAA
AAGGTGGTGA TGACAGCCTT CGCAGACCGC ACTGTGGTCA CCATCGCGCA TCGAGTGCAC
ACCATCCTGA GTGCAGACCT GGTGATCGTC CTGAAGCGGG GTGCCATCCT TGAGTTCGAT
AAGCCAGAGA AGCTGCTCAG CCGGAAGGAC AGCGTCTTCG CCTCCTTCGT CCGTGCAGAC
AAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60819
Clone ID Related Accession (Same CDS sequence) XM_011520334
Accession Version XM_011520334.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2946bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ATP-binding cassette sub-family C member 8 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009237.19) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
ATGCCCCTGG CCTTCTGCGG CAGCGAGAAC CACTCGGCCG CCTACCGGGT GGACCAGGGG 
GTCCTCAACA ACGGCTGCTT TGTGGACGCG CTCAACGTGG TGCCGCACGT CTTCCTACTC
TTCATCACCT TCCCCATCCT CTTCATTGGA TGGGGAAGTC AGAGCTCCAA GGTGCACATC
CACCACAGCA CATGGCTTCA TTTCCCTGGG CACAACCTGC GGTGGATCCT GACCTTCATG
CTGCTCTTCG TCCTGGTGTG TGAGATTGCA GAGGGCATCC TGTCTGATGG GGTGACCGAA
TCCCACCATC TGCACCTGTA CATGCCAGCC GGGATGGCGT TCATGGCTGC TGTCACCTCC
GTGGTCTACT ATCACAACAT CGAGACTTCC AACTTCCCCA AGCTGCTAAT TGCCCTGCTG
GTGTATTGGA CCCTGGCCTT CATCACCAAG ACCATCAAGT TTGTCAAGTT CTTGGACCAC
GCCATCGGCT TCTCGCAGCT ACGCTTCTGC CTCACAGGGC TGCTGGTGAT CCTCTATGGG
ATGCTGCTCC TCGTGGAGGT CAATGTCATC AGGGTGAGGA GATACATCTT CTTCAAGACA
CCGAGGGAGG TGAAGCCTCC CGAGGACCTG CAAGACCTGG GGGTACGCTT CCTGCAGCCC
TTCGTGAATC TGCTGTCCAA AGGCACCTAC TGGTGGATGA ACGCCTTCAT CAAGACTGCC
CACAAGAAGC CCATCGACTT GCGAGCCATC GGGAAGCTGC CCATCGCCAT GAGGGCCCTC
ACCAACTACC AACGGCTCTG CGAGGCCTTT GACGCCCAGG TGCGGAAGGA CATTCAGGGC
ACTCAAGGTG CCCGGGCCAT CTGGCAGGCA CTCAGCCATG CCTTCGGGAG GCGCCTGGTC
CTCAGCAGCA CTTTCCGCAT CTTGGCCGAC CTGCTGGGCT TCGCCGGGCC ACTGTGCATC
TTTGGGATCG TGGACCACCT TGGGAAGGAG AACGACGTCT TCCAGCCCAA GACACAATTT
CTCGGGGTTT ACTTTGTCTC ATCCCAAGAG TTCCTTGCCA ATGCCTACGT CTTAGCTGTG
CTTCTGTTCC TTGCCCTCCT ACTGCAAAGG ACATTTCTGC AAGCATCCTA CTATGTGGCC
ATTGAAACTG GAATTAACTT GAGAGGAGCA ATACAGACCA AGATTTACAA TAAAATTATG
CACCTGTCCA CCTCCAACCT GTCCATGGGA GAAATGACTG CTGGACAGAT CTGTAATCTG
GTTGCCATCG ACACCAATCA GCTCATGTGG TTTTTCTTCT TGTGCCCAAA CCTCTGGGCT
ATGCCAGTAC AGATCATTGT GGGTGTGATT CTCCTCTACT ACATACTCGG AGTCAGTGCC
TTAATTGGAG CAGCTGTCAT CATTCTACTG GCTCCTGTCC AGTACTTCGT GGCCACCAAG
CTGTCTCAGG CCCAGCGGAG CACACTGGAG TATTCCAATG AGCGGCTGAA GCAGACCAAC
GAGATGCTCC GCGGCATCAA GCTGCTGAAG CTGTACGCCT GGGAGAACAT CTTCCGCACG
CGGGTGGAGA CGACCCGCAG GAAGGAGATG ACCAGCCTCA GGGCCTTTGC CATCTATACC
TCCATCTCCA TTTTCATGAA CACGGCCATC CCCATTGCAG CTGTCCTCAT AACTTTCGTG
GGCCACGTCA GCTTCTTCAA AGAGGCCGAC TTCTCGCCCT CCGTGGCCTT TGCCTCCCTC
TCCCTCTTCC ATATCTTGGT CACACCGCTG TTCCTGCTGT CCAGTGTGGT CCGATCTACC
GTCAAAGCTC TAGTGAGCGT GCAAAAGCTA AGCGAGTTCC TGTCCAGTGC AGAGATCCGT
GAGGAGCAGT GTGCCCCCCA TGAGCCCACA CCTCAGGGCC CAGCCAGCAA GTACCAGGCG
GTGCCCCTCA GGGTTGTGAA CCGCAAGCGT CCAGCCCGGG AGGATTGTCG GGGCCTCACC
GGCCCACTGC AGAGCCTGGT CCCCAGTGCA GATGGCGATG CTGACAACTG CTGTGTCCAG
ATCATGGGAG GCTACTTCAC GTGGACCCCA GATGGAATCC CCACACTGTC CAACATCACC
ATTCGTATCC CCCGAGGCCA GCTGACTATG ATCGTGGGGC AGGTGGGCTG CGGCAAGTCC
TCGCTCCTTC TAGCCGCACT GGGGGAGATG CAGAAGGTCT CAGGGGCTGT CTTCTGGAGC
AGCAGCCTTC CTGACAGCGA GATAGGAGAG GACCCCAGCC CAGAGCGGGA GACAGCGACC
GACTTGGATA TCAGGAAGAG AGGCCCCGTG GCCTATGCTT CGCAGAAACC ATGGCTGCTA
AATGCCACTG TGGAGGAGAA CATCATCTTT GAGAGTCCCT TCAACAAACA ACGGTACAAG
ATGGTCATTG AAGCCTGCTC TCTGCAGCCA GACATCGACA TCCTGCCCCA TGGAGACCAG
ACCCAGATTG GGGAACGGGG CATCAACCTG TCTGGTGGTC AACGCCAGCG AATCAGTGTG
GCCCGAGCCC TCTACCAGCA CGCCAACGTT GTCTTCTTGG ATGACCCCTT CTCAGCTCTG
GATATCCATC TGAGTGACCA CTTAATGCAG GCCGGCATCC TTGAGCTGCT CCGGGACGAC
AAGAGGACAG TGGTCTTAGT GACCCACAAG CTACAGTACC TGCCCCATGC AGACTGGATC
ATTGCCATGA AGGATGGCAC CATCCAGAGG GAGGGTACCC TCAAGGACTT CCAGAGGTCT
GAATGCCAGC TCTTTGAGCA CTGGAAGACC CTCATGAACC GACAGGACCA AGAGCTGGAG
AAGGAGACTG TCACAGAGAG AAAAGCCACA GAGCCACCCC AGGGCCTATC TCGTGCCATG
TCCTCGAGGG ATGGCCTTCT GCAGGATGAG GAAGAGGAGG AAGTAAGTGG GGATAAAATG
CCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011518636.1
CDS64..3009
Misc Feature(1)922..2862(+)
Misc Feature(2)1015..1833(+)
Misc Feature(3)2098..2784(+)
Misc Feature(4)2200..2223(+)
Misc Feature(5)2209..2730(+)
Misc Feature(6)2377..2388(+)
Misc Feature(7)2551..2580(+)
Misc Feature(8)2611..2628(+)
Misc Feature(9)2635..2646(+)
Misc Feature(10)2716..2736(+)
Translation

Target ORF information:

RefSeq Version XM_011520334
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens ATP-binding cassette, sub-family C (CFTR/MRP), member 8 (ABCC8), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011520334

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
ATGCCCCTGG CCTTCTGCGG CAGCGAGAAC CACTCGGCCG CCTACCGGGT GGACCAGGGG 
GTCCTCAACA ACGGCTGCTT TGTGGACGCG CTCAACGTGG TGCCGCACGT CTTCCTACTC
TTCATCACCT TCCCCATCCT CTTCATTGGA TGGGGAAGTC AGAGCTCCAA GGTGCACATC
CACCACAGCA CATGGCTTCA TTTCCCTGGG CACAACCTGC GGTGGATCCT GACCTTCATG
CTGCTCTTCG TCCTGGTGTG TGAGATTGCA GAGGGCATCC TGTCTGATGG GGTGACCGAA
TCCCACCATC TGCACCTGTA CATGCCAGCC GGGATGGCGT TCATGGCTGC TGTCACCTCC
GTGGTCTACT ATCACAACAT CGAGACTTCC AACTTCCCCA AGCTGCTAAT TGCCCTGCTG
GTGTATTGGA CCCTGGCCTT CATCACCAAG ACCATCAAGT TTGTCAAGTT CTTGGACCAC
GCCATCGGCT TCTCGCAGCT ACGCTTCTGC CTCACAGGGC TGCTGGTGAT CCTCTATGGG
ATGCTGCTCC TCGTGGAGGT CAATGTCATC AGGGTGAGGA GATACATCTT CTTCAAGACA
CCGAGGGAGG TGAAGCCTCC CGAGGACCTG CAAGACCTGG GGGTACGCTT CCTGCAGCCC
TTCGTGAATC TGCTGTCCAA AGGCACCTAC TGGTGGATGA ACGCCTTCAT CAAGACTGCC
CACAAGAAGC CCATCGACTT GCGAGCCATC GGGAAGCTGC CCATCGCCAT GAGGGCCCTC
ACCAACTACC AACGGCTCTG CGAGGCCTTT GACGCCCAGG TGCGGAAGGA CATTCAGGGC
ACTCAAGGTG CCCGGGCCAT CTGGCAGGCA CTCAGCCATG CCTTCGGGAG GCGCCTGGTC
CTCAGCAGCA CTTTCCGCAT CTTGGCCGAC CTGCTGGGCT TCGCCGGGCC ACTGTGCATC
TTTGGGATCG TGGACCACCT TGGGAAGGAG AACGACGTCT TCCAGCCCAA GACACAATTT
CTCGGGGTTT ACTTTGTCTC ATCCCAAGAG TTCCTTGCCA ATGCCTACGT CTTAGCTGTG
CTTCTGTTCC TTGCCCTCCT ACTGCAAAGG ACATTTCTGC AAGCATCCTA CTATGTGGCC
ATTGAAACTG GAATTAACTT GAGAGGAGCA ATACAGACCA AGATTTACAA TAAAATTATG
CACCTGTCCA CCTCCAACCT GTCCATGGGA GAAATGACTG CTGGACAGAT CTGTAATCTG
GTTGCCATCG ACACCAATCA GCTCATGTGG TTTTTCTTCT TGTGCCCAAA CCTCTGGGCT
ATGCCAGTAC AGATCATTGT GGGTGTGATT CTCCTCTACT ACATACTCGG AGTCAGTGCC
TTAATTGGAG CAGCTGTCAT CATTCTACTG GCTCCTGTCC AGTACTTCGT GGCCACCAAG
CTGTCTCAGG CCCAGCGGAG CACACTGGAG TATTCCAATG AGCGGCTGAA GCAGACCAAC
GAGATGCTCC GCGGCATCAA GCTGCTGAAG CTGTACGCCT GGGAGAACAT CTTCCGCACG
CGGGTGGAGA CGACCCGCAG GAAGGAGATG ACCAGCCTCA GGGCCTTTGC CATCTATACC
TCCATCTCCA TTTTCATGAA CACGGCCATC CCCATTGCAG CTGTCCTCAT AACTTTCGTG
GGCCACGTCA GCTTCTTCAA AGAGGCCGAC TTCTCGCCCT CCGTGGCCTT TGCCTCCCTC
TCCCTCTTCC ATATCTTGGT CACACCGCTG TTCCTGCTGT CCAGTGTGGT CCGATCTACC
GTCAAAGCTC TAGTGAGCGT GCAAAAGCTA AGCGAGTTCC TGTCCAGTGC AGAGATCCGT
GAGGAGCAGT GTGCCCCCCA TGAGCCCACA CCTCAGGGCC CAGCCAGCAA GTACCAGGCG
GTGCCCCTCA GGGTTGTGAA CCGCAAGCGT CCAGCCCGGG AGGATTGTCG GGGCCTCACC
GGCCCACTGC AGAGCCTGGT CCCCAGTGCA GATGGCGATG CTGACAACTG CTGTGTCCAG
ATCATGGGAG GCTACTTCAC GTGGACCCCA GATGGAATCC CCACACTGTC CAACATCACC
ATTCGTATCC CCCGAGGCCA GCTGACTATG ATCGTGGGGC AGGTGGGCTG CGGCAAGTCC
TCGCTCCTTC TAGCCGCACT GGGGGAGATG CAGAAGGTCT CAGGGGCTGT CTTCTGGAGC
AGCAGCCTTC CTGACAGCGA GATAGGAGAG GACCCCAGCC CAGAGCGGGA GACAGCGACC
GACTTGGATA TCAGGAAGAG AGGCCCCGTG GCCTATGCTT CGCAGAAACC ATGGCTGCTA
AATGCCACTG TGGAGGAGAA CATCATCTTT GAGAGTCCCT TCAACAAACA ACGGTACAAG
ATGGTCATTG AAGCCTGCTC TCTGCAGCCA GACATCGACA TCCTGCCCCA TGGAGACCAG
ACCCAGATTG GGGAACGGGG CATCAACCTG TCTGGTGGTC AACGCCAGCG AATCAGTGTG
GCCCGAGCCC TCTACCAGCA CGCCAACGTT GTCTTCTTGG ATGACCCCTT CTCAGCTCTG
GATATCCATC TGAGTGACCA CTTAATGCAG GCCGGCATCC TTGAGCTGCT CCGGGACGAC
AAGAGGACAG TGGTCTTAGT GACCCACAAG CTACAGTACC TGCCCCATGC AGACTGGATC
ATTGCCATGA AGGATGGCAC CATCCAGAGG GAGGGTACCC TCAAGGACTT CCAGAGGTCT
GAATGCCAGC TCTTTGAGCA CTGGAAGACC CTCATGAACC GACAGGACCA AGAGCTGGAG
AAGGAGACTG TCACAGAGAG AAAAGCCACA GAGCCACCCC AGGGCCTATC TCGTGCCATG
TCCTCGAGGG ATGGCCTTCT GCAGGATGAG GAAGAGGAGG AAGTAAGTGG GGATAAAATG
CCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21635
Clone ID Related Accession (Same CDS sequence) NM_000352
Accession Version NM_000352.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4746bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product ATP-binding cassette sub-family C member 8 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC124798.5, AF087138.1, R84665.1, BM352492.1 and BM313666.1. On Dec 3, 2013 this sequence version replaced gi:118582254. Transcript Variant: This variant (2) uses an alternate in-frame acceptor splice site at an internal coding exon compared to variant 1. The resulting isoform (2) is 1 aa shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L78207.1, U63421.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
ATGCCCCTGG CCTTCTGCGG CAGCGAGAAC CACTCGGCCG CCTACCGGGT GGACCAGGGG 
GTCCTCAACA ACGGCTGCTT TGTGGACGCG CTCAACGTGG TGCCGCACGT CTTCCTACTC
TTCATCACCT TCCCCATCCT CTTCATTGGA TGGGGAAGTC AGAGCTCCAA GGTGCACATC
CACCACAGCA CATGGCTTCA TTTCCCTGGG CACAACCTGC GGTGGATCCT GACCTTCATG
CTGCTCTTCG TCCTGGTGTG TGAGATTGCA GAGGGCATCC TGTCTGATGG GGTGACCGAA
TCCCACCATC TGCACCTGTA CATGCCAGCC GGGATGGCGT TCATGGCTGC TGTCACCTCC
GTGGTCTACT ATCACAACAT CGAGACTTCC AACTTCCCCA AGCTGCTAAT TGCCCTGCTG
GTGTATTGGA CCCTGGCCTT CATCACCAAG ACCATCAAGT TTGTCAAGTT CTTGGACCAC
GCCATCGGCT TCTCGCAGCT ACGCTTCTGC CTCACAGGGC TGCTGGTGAT CCTCTATGGG
ATGCTGCTCC TCGTGGAGGT CAATGTCATC AGGGTGAGGA GATACATCTT CTTCAAGACA
CCGAGGGAGG TGAAGCCTCC CGAGGACCTG CAAGACCTGG GGGTACGCTT CCTGCAGCCC
TTCGTGAATC TGCTGTCCAA AGGCACCTAC TGGTGGATGA ACGCCTTCAT CAAGACTGCC
CACAAGAAGC CCATCGACTT GCGAGCCATC GGGAAGCTGC CCATCGCCAT GAGGGCCCTC
ACCAACTACC AACGGCTCTG CGAGGCCTTT GACGCCCAGG TGCGGAAGGA CATTCAGGGC
ACTCAAGGTG CCCGGGCCAT CTGGCAGGCA CTCAGCCATG CCTTCGGGAG GCGCCTGGTC
CTCAGCAGCA CTTTCCGCAT CTTGGCCGAC CTGCTGGGCT TCGCCGGGCC ACTGTGCATC
TTTGGGATCG TGGACCACCT TGGGAAGGAG AACGACGTCT TCCAGCCCAA GACACAATTT
CTCGGGGTTT ACTTTGTCTC ATCCCAAGAG TTCCTTGCCA ATGCCTACGT CTTAGCTGTG
CTTCTGTTCC TTGCCCTCCT ACTGCAAAGG ACATTTCTGC AAGCATCCTA CTATGTGGCC
ATTGAAACTG GAATTAACTT GAGAGGAGCA ATACAGACCA AGATTTACAA TAAAATTATG
CACCTGTCCA CCTCCAACCT GTCCATGGGA GAAATGACTG CTGGACAGAT CTGTAATCTG
GTTGCCATCG ACACCAATCA GCTCATGTGG TTTTTCTTCT TGTGCCCAAA CCTCTGGGCT
ATGCCAGTAC AGATCATTGT GGGTGTGATT CTCCTCTACT ACATACTCGG AGTCAGTGCC
TTAATTGGAG CAGCTGTCAT CATTCTACTG GCTCCTGTCC AGTACTTCGT GGCCACCAAG
CTGTCTCAGG CCCAGCGGAG CACACTGGAG TATTCCAATG AGCGGCTGAA GCAGACCAAC
GAGATGCTCC GCGGCATCAA GCTGCTGAAG CTGTACGCCT GGGAGAACAT CTTCCGCACG
CGGGTGGAGA CGACCCGCAG GAAGGAGATG ACCAGCCTCA GGGCCTTTGC CATCTATACC
TCCATCTCCA TTTTCATGAA CACGGCCATC CCCATTGCAG CTGTCCTCAT AACTTTCGTG
GGCCACGTCA GCTTCTTCAA AGAGGCCGAC TTCTCGCCCT CCGTGGCCTT TGCCTCCCTC
TCCCTCTTCC ATATCTTGGT CACACCGCTG TTCCTGCTGT CCAGTGTGGT CCGATCTACC
GTCAAAGCTC TAGTGAGCGT GCAAAAGCTA AGCGAGTTCC TGTCCAGTGC AGAGATCCGT
GAGGAGCAGT GTGCCCCCCA TGAGCCCACA CCTCAGGGCC CAGCCAGCAA GTACCAGGCG
GTGCCCCTCA GGGTTGTGAA CCGCAAGCGT CCAGCCCGGG AGGATTGTCG GGGCCTCACC
GGCCCACTGC AGAGCCTGGT CCCCAGTGCA GATGGCGATG CTGACAACTG CTGTGTCCAG
ATCATGGGAG GCTACTTCAC GTGGACCCCA GATGGAATCC CCACACTGTC CAACATCACC
ATTCGTATCC CCCGAGGCCA GCTGACTATG ATCGTGGGGC AGGTGGGCTG CGGCAAGTCC
TCGCTCCTTC TAGCCGCACT GGGGGAGATG CAGAAGGTCT CAGGGGCTGT CTTCTGGAGC
AGCCTTCCTG ACAGCGAGAT AGGAGAGGAC CCCAGCCCAG AGCGGGAGAC AGCGACCGAC
TTGGATATCA GGAAGAGAGG CCCCGTGGCC TATGCTTCGC AGAAACCATG GCTGCTAAAT
GCCACTGTGG AGGAGAACAT CATCTTTGAG AGTCCCTTCA ACAAACAACG GTACAAGATG
GTCATTGAAG CCTGCTCTCT GCAGCCAGAC ATCGACATCC TGCCCCATGG AGACCAGACC
CAGATTGGGG AACGGGGCAT CAACCTGTCT GGTGGTCAAC GCCAGCGAAT CAGTGTGGCC
CGAGCCCTCT ACCAGCACGC CAACGTTGTC TTCTTGGATG ACCCCTTCTC AGCTCTGGAT
ATCCATCTGA GTGACCACTT AATGCAGGCC GGCATCCTTG AGCTGCTCCG GGACGACAAG
AGGACAGTGG TCTTAGTGAC CCACAAGCTA CAGTACCTGC CCCATGCAGA CTGGATCATT
GCCATGAAGG ATGGCACCAT CCAGAGGGAG GGTACCCTCA AGGACTTCCA GAGGTCTGAA
TGCCAGCTCT TTGAGCACTG GAAGACCCTC ATGAACCGAC AGGACCAAGA GCTGGAGAAG
GAGACTGTCA CAGAGAGAAA AGCCACAGAG CCACCCCAGG GCCTATCTCG TGCCATGTCC
TCGAGGGATG GCCTTCTGCA GGATGAGGAA GAGGAGGAAG AGGAGGCAGC TGAGAGCGAG
GAGGATGACA ACCTGTCGTC CATGCTGCAC CAGCGTGCTG AGATCCCATG GCGAGCCTGC
GCCAAGTACC TGTCCTCCGC CGGCATCCTG CTCCTGTCGT TGCTGGTCTT CTCACAGCTG
CTCAAGCACA TGGTCCTGGT GGCCATCGAC TACTGGCTGG CCAAGTGGAC CGACAGCGCC
CTGACCCTGA CCCCTGCAGC CAGGAACTGC TCCCTCAGCC AGGAGTGCAC CCTCGACCAG
ACTGTCTATG CCATGGTGTT CACGGTGCTC TGCAGCCTGG GCATTGTGCT GTGCCTCGTC
ACGTCTGTCA CTGTGGAGTG GACAGGGCTG AAGGTGGCCA AGAGACTGCA CCGCAGCCTG
CTAAACCGGA TCATCCTAGC CCCCATGAGG TTTTTTGAGA CCACGCCCCT TGGGAGCATC
CTGAACAGAT TTTCATCTGA CTGTAACACC ATCGACCAGC ACATCCCATC CACGCTGGAG
TGCCTGAGCC GCTCCACCCT GCTCTGTGTC TCAGCCCTGG CCGTCATCTC CTATGTCACA
CCTGTGTTCC TCGTGGCCCT CTTGCCCCTG GCCATCGTGT GCTACTTCAT CCAGAAGTAC
TTCCGGGTGG CGTCCAGGGA CCTGCAGCAG CTGGATGACA CCACCCAGCT TCCACTTCTC
TCACACTTTG CCGAAACCGT AGAAGGACTC ACCACCATCC GGGCCTTCAG GTATGAGGCC
CGGTTCCAGC AGAAGCTTCT CGAATACACA GACTCCAACA ACATTGCTTC CCTCTTCCTC
ACAGCTGCCA ACAGATGGCT GGAAGTCCGA ATGGAGTACA TCGGTGCATG TGTGGTGCTC
ATCGCAGCGG TGACCTCCAT CTCCAACTCC CTGCACAGGG AGCTCTCTGC TGGCCTGGTG
GGCCTGGGCC TTACCTACGC CCTAATGGTC TCCAACTACC TCAACTGGAT GGTGAGGAAC
CTGGCAGACA TGGAGCTCCA GCTGGGGGCT GTGAAGCGCA TCCATGGGCT CCTGAAAACC
GAGGCAGAGA GCTACGAGGG GCTCCTGGCA CCATCGCTGA TCCCAAAGAA CTGGCCAGAC
CAAGGGAAGA TCCAGATCCA GAACCTGAGC GTGCGCTACG ACAGCTCCCT GAAGCCGGTG
CTGAAGCACG TCAATGCCCT CATCGCCCCT GGACAGAAGA TCGGGATCTG CGGCCGCACC
GGCAGTGGGA AGTCCTCCTT CTCTCTTGCC TTCTTCCGCA TGGTGGACAC GTTCGAAGGG
CACATCATCA TTGATGGCAT TGACATCGCC AAACTGCCGC TGCACACCCT GCGCTCACGC
CTCTCCATCA TCCTGCAGGA CCCCGTCCTC TTCAGCGGCA CCATCCGATT TAACCTGGAC
CCTGAGAGGA AGTGCTCAGA TAGCACACTG TGGGAGGCCC TGGAAATCGC CCAGCTGAAG
CTGGTGGTGA AGGCACTGCC AGGAGGCCTC GATGCCATCA TCACAGAAGG CGGGGAGAAT
TTCAGCCAGG GACAGAGGCA GCTGTTCTGC CTGGCCCGGG CCTTCGTGAG GAAGACCAGC
ATCTTCATCA TGGACGAGGC CACGGCTTCC ATTGACATGG CCACGGAAAA CATCCTCCAA
AAGGTGGTGA TGACAGCCTT CGCAGACCGC ACTGTGGTCA CCATCGCGCA TCGAGTGCAC
ACCATCCTGA GTGCAGACCT GGTGATCGTC CTGAAGCGGG GTGCCATCCT TGAGTTCGAT
AAGCCAGAGA AGCTGCTCAG CCGGAAGGAC AGCGTCTTCG CCTCCTTCGT CCGTGCAGAC
AAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000343.2
CDS70..4815
Misc Feature(1)172..234(+)
Misc Feature(2)295..357(+)
Misc Feature(3)373..435(+)
Misc Feature(4)472..531(+)
Misc Feature(5)571..651(+)
Misc Feature(6)736..4812(+)
Misc Feature(7)1003..1062(+)
Misc Feature(8)1021..1839(+)
Misc Feature(9)1135..1197(+)
Misc Feature(10)1372..1434(+)
Misc Feature(11)1444..1506(+)
Misc Feature(12)1693..1755(+)
Misc Feature(13)1822..1884(+)
Misc Feature(14)2104..2787(+)
Misc Feature(15)2206..2229(+)
Misc Feature(16)2215..2733(+)
Misc Feature(17)2380..2391(+)
Misc Feature(18)2554..2583(+)
Misc Feature(19)2614..2631(+)
Misc Feature(20)2638..2649(+)
Misc Feature(21)2719..2739(+)
Misc Feature(22)3082..3144(+)
Misc Feature(23)3103..3951(+)
Misc Feature(24)3286..3348(+)
Misc Feature(25)3481..3543(+)
Misc Feature(26)3547..3609(+)
Misc Feature(27)3823..3885(+)
Misc Feature(28)3898..3960(+)
Misc Feature(29)4093..4812(+)
Misc Feature(30)4201..4224(+)
Misc Feature(31)4210..4689(+)
Misc Feature(32)4336..4347(+)
Misc Feature(33)4510..4539(+)
Misc Feature(34)4570..4587(+)
Misc Feature(35)4594..4605(+)
Misc Feature(36)4675..4695(+)
Exon (1)1..217
Gene:ABCC8
Gene Synonym:
Exon (2)218..359
Gene:ABCC8
Gene Synonym:
Exon (3)360..481
Gene:ABCC8
Gene Synonym:
Exon (4)482..648
Gene:ABCC8
Gene Synonym:
Exon (5)649..891
Gene:ABCC8
Gene Synonym:
Exon (6)892..1080
Gene:ABCC8
Gene Synonym:
Exon (7)1081..1245
Gene:ABCC8
Gene Synonym:
Exon (8)1246..1401
Gene:ABCC8
Gene Synonym:
Exon (9)1402..1536
Gene:ABCC8
Gene Synonym:
Exon (10)1537..1699
Gene:ABCC8
Gene Synonym:
Exon (11)1700..1740
Gene:ABCC8
Gene Synonym:
Exon (12)1741..1886
Gene:ABCC8
Gene Synonym:
Exon (13)1887..1992
Gene:ABCC8
Gene Synonym:
Exon (14)1993..2109
Gene:ABCC8
Gene Synonym:
Exon (15)2110..2185
Gene:ABCC8
Gene Synonym:
Exon (16)2186..2291
Gene:ABCC8
Gene Synonym:
Exon (17)2292..2324
Gene:ABCC8
Gene Synonym:
Exon (18)2325..2360
Gene:ABCC8
Gene Synonym:
Exon (19)2361..2459
Gene:ABCC8
Gene Synonym:
Exon (20)2460..2544
Gene:ABCC8
Gene Synonym:
Exon (21)2545..2625
Gene:ABCC8
Gene Synonym:
Exon (22)2626..2763
Gene:ABCC8
Gene Synonym:
Exon (23)2764..2889
Gene:ABCC8
Gene Synonym:
Exon (24)2890..2989
Gene:ABCC8
Gene Synonym:
Exon (25)2990..3231
Gene:ABCC8
Gene Synonym:
Exon (26)3232..3398
Gene:ABCC8
Gene Synonym:
Exon (27)3399..3468
Gene:ABCC8
Gene Synonym:
Exon (28)3469..3626
Gene:ABCC8
Gene Synonym:
Exon (29)3627..3719
Gene:ABCC8
Gene Synonym:
Exon (30)3720..3822
Gene:ABCC8
Gene Synonym:
Exon (31)3823..3936
Gene:ABCC8
Gene Synonym:
Exon (32)3937..4057
Gene:ABCC8
Gene Synonym:
Exon (33)4058..4188
Gene:ABCC8
Gene Synonym:
Exon (34)4189..4267
Gene:ABCC8
Gene Synonym:
Exon (35)4268..4376
Gene:ABCC8
Gene Synonym:
Exon (36)4377..4480
Gene:ABCC8
Gene Synonym:
Exon (37)4481..4614
Gene:ABCC8
Gene Synonym:
Exon (38)4615..4677
Gene:ABCC8
Gene Synonym:
Exon (39)4678..4921
Gene:ABCC8
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000352
Organism Homo sapiens (human)
Definition Homo sapiens ATP-binding cassette, sub-family C (CFTR/MRP), member 8 (ABCC8), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000352

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
ATGCCCCTGG CCTTCTGCGG CAGCGAGAAC CACTCGGCCG CCTACCGGGT GGACCAGGGG 
GTCCTCAACA ACGGCTGCTT TGTGGACGCG CTCAACGTGG TGCCGCACGT CTTCCTACTC
TTCATCACCT TCCCCATCCT CTTCATTGGA TGGGGAAGTC AGAGCTCCAA GGTGCACATC
CACCACAGCA CATGGCTTCA TTTCCCTGGG CACAACCTGC GGTGGATCCT GACCTTCATG
CTGCTCTTCG TCCTGGTGTG TGAGATTGCA GAGGGCATCC TGTCTGATGG GGTGACCGAA
TCCCACCATC TGCACCTGTA CATGCCAGCC GGGATGGCGT TCATGGCTGC TGTCACCTCC
GTGGTCTACT ATCACAACAT CGAGACTTCC AACTTCCCCA AGCTGCTAAT TGCCCTGCTG
GTGTATTGGA CCCTGGCCTT CATCACCAAG ACCATCAAGT TTGTCAAGTT CTTGGACCAC
GCCATCGGCT TCTCGCAGCT ACGCTTCTGC CTCACAGGGC TGCTGGTGAT CCTCTATGGG
ATGCTGCTCC TCGTGGAGGT CAATGTCATC AGGGTGAGGA GATACATCTT CTTCAAGACA
CCGAGGGAGG TGAAGCCTCC CGAGGACCTG CAAGACCTGG GGGTACGCTT CCTGCAGCCC
TTCGTGAATC TGCTGTCCAA AGGCACCTAC TGGTGGATGA ACGCCTTCAT CAAGACTGCC
CACAAGAAGC CCATCGACTT GCGAGCCATC GGGAAGCTGC CCATCGCCAT GAGGGCCCTC
ACCAACTACC AACGGCTCTG CGAGGCCTTT GACGCCCAGG TGCGGAAGGA CATTCAGGGC
ACTCAAGGTG CCCGGGCCAT CTGGCAGGCA CTCAGCCATG CCTTCGGGAG GCGCCTGGTC
CTCAGCAGCA CTTTCCGCAT CTTGGCCGAC CTGCTGGGCT TCGCCGGGCC ACTGTGCATC
TTTGGGATCG TGGACCACCT TGGGAAGGAG AACGACGTCT TCCAGCCCAA GACACAATTT
CTCGGGGTTT ACTTTGTCTC ATCCCAAGAG TTCCTTGCCA ATGCCTACGT CTTAGCTGTG
CTTCTGTTCC TTGCCCTCCT ACTGCAAAGG ACATTTCTGC AAGCATCCTA CTATGTGGCC
ATTGAAACTG GAATTAACTT GAGAGGAGCA ATACAGACCA AGATTTACAA TAAAATTATG
CACCTGTCCA CCTCCAACCT GTCCATGGGA GAAATGACTG CTGGACAGAT CTGTAATCTG
GTTGCCATCG ACACCAATCA GCTCATGTGG TTTTTCTTCT TGTGCCCAAA CCTCTGGGCT
ATGCCAGTAC AGATCATTGT GGGTGTGATT CTCCTCTACT ACATACTCGG AGTCAGTGCC
TTAATTGGAG CAGCTGTCAT CATTCTACTG GCTCCTGTCC AGTACTTCGT GGCCACCAAG
CTGTCTCAGG CCCAGCGGAG CACACTGGAG TATTCCAATG AGCGGCTGAA GCAGACCAAC
GAGATGCTCC GCGGCATCAA GCTGCTGAAG CTGTACGCCT GGGAGAACAT CTTCCGCACG
CGGGTGGAGA CGACCCGCAG GAAGGAGATG ACCAGCCTCA GGGCCTTTGC CATCTATACC
TCCATCTCCA TTTTCATGAA CACGGCCATC CCCATTGCAG CTGTCCTCAT AACTTTCGTG
GGCCACGTCA GCTTCTTCAA AGAGGCCGAC TTCTCGCCCT CCGTGGCCTT TGCCTCCCTC
TCCCTCTTCC ATATCTTGGT CACACCGCTG TTCCTGCTGT CCAGTGTGGT CCGATCTACC
GTCAAAGCTC TAGTGAGCGT GCAAAAGCTA AGCGAGTTCC TGTCCAGTGC AGAGATCCGT
GAGGAGCAGT GTGCCCCCCA TGAGCCCACA CCTCAGGGCC CAGCCAGCAA GTACCAGGCG
GTGCCCCTCA GGGTTGTGAA CCGCAAGCGT CCAGCCCGGG AGGATTGTCG GGGCCTCACC
GGCCCACTGC AGAGCCTGGT CCCCAGTGCA GATGGCGATG CTGACAACTG CTGTGTCCAG
ATCATGGGAG GCTACTTCAC GTGGACCCCA GATGGAATCC CCACACTGTC CAACATCACC
ATTCGTATCC CCCGAGGCCA GCTGACTATG ATCGTGGGGC AGGTGGGCTG CGGCAAGTCC
TCGCTCCTTC TAGCCGCACT GGGGGAGATG CAGAAGGTCT CAGGGGCTGT CTTCTGGAGC
AGCCTTCCTG ACAGCGAGAT AGGAGAGGAC CCCAGCCCAG AGCGGGAGAC AGCGACCGAC
TTGGATATCA GGAAGAGAGG CCCCGTGGCC TATGCTTCGC AGAAACCATG GCTGCTAAAT
GCCACTGTGG AGGAGAACAT CATCTTTGAG AGTCCCTTCA ACAAACAACG GTACAAGATG
GTCATTGAAG CCTGCTCTCT GCAGCCAGAC ATCGACATCC TGCCCCATGG AGACCAGACC
CAGATTGGGG AACGGGGCAT CAACCTGTCT GGTGGTCAAC GCCAGCGAAT CAGTGTGGCC
CGAGCCCTCT ACCAGCACGC CAACGTTGTC TTCTTGGATG ACCCCTTCTC AGCTCTGGAT
ATCCATCTGA GTGACCACTT AATGCAGGCC GGCATCCTTG AGCTGCTCCG GGACGACAAG
AGGACAGTGG TCTTAGTGAC CCACAAGCTA CAGTACCTGC CCCATGCAGA CTGGATCATT
GCCATGAAGG ATGGCACCAT CCAGAGGGAG GGTACCCTCA AGGACTTCCA GAGGTCTGAA
TGCCAGCTCT TTGAGCACTG GAAGACCCTC ATGAACCGAC AGGACCAAGA GCTGGAGAAG
GAGACTGTCA CAGAGAGAAA AGCCACAGAG CCACCCCAGG GCCTATCTCG TGCCATGTCC
TCGAGGGATG GCCTTCTGCA GGATGAGGAA GAGGAGGAAG AGGAGGCAGC TGAGAGCGAG
GAGGATGACA ACCTGTCGTC CATGCTGCAC CAGCGTGCTG AGATCCCATG GCGAGCCTGC
GCCAAGTACC TGTCCTCCGC CGGCATCCTG CTCCTGTCGT TGCTGGTCTT CTCACAGCTG
CTCAAGCACA TGGTCCTGGT GGCCATCGAC TACTGGCTGG CCAAGTGGAC CGACAGCGCC
CTGACCCTGA CCCCTGCAGC CAGGAACTGC TCCCTCAGCC AGGAGTGCAC CCTCGACCAG
ACTGTCTATG CCATGGTGTT CACGGTGCTC TGCAGCCTGG GCATTGTGCT GTGCCTCGTC
ACGTCTGTCA CTGTGGAGTG GACAGGGCTG AAGGTGGCCA AGAGACTGCA CCGCAGCCTG
CTAAACCGGA TCATCCTAGC CCCCATGAGG TTTTTTGAGA CCACGCCCCT TGGGAGCATC
CTGAACAGAT TTTCATCTGA CTGTAACACC ATCGACCAGC ACATCCCATC CACGCTGGAG
TGCCTGAGCC GCTCCACCCT GCTCTGTGTC TCAGCCCTGG CCGTCATCTC CTATGTCACA
CCTGTGTTCC TCGTGGCCCT CTTGCCCCTG GCCATCGTGT GCTACTTCAT CCAGAAGTAC
TTCCGGGTGG CGTCCAGGGA CCTGCAGCAG CTGGATGACA CCACCCAGCT TCCACTTCTC
TCACACTTTG CCGAAACCGT AGAAGGACTC ACCACCATCC GGGCCTTCAG GTATGAGGCC
CGGTTCCAGC AGAAGCTTCT CGAATACACA GACTCCAACA ACATTGCTTC CCTCTTCCTC
ACAGCTGCCA ACAGATGGCT GGAAGTCCGA ATGGAGTACA TCGGTGCATG TGTGGTGCTC
ATCGCAGCGG TGACCTCCAT CTCCAACTCC CTGCACAGGG AGCTCTCTGC TGGCCTGGTG
GGCCTGGGCC TTACCTACGC CCTAATGGTC TCCAACTACC TCAACTGGAT GGTGAGGAAC
CTGGCAGACA TGGAGCTCCA GCTGGGGGCT GTGAAGCGCA TCCATGGGCT CCTGAAAACC
GAGGCAGAGA GCTACGAGGG GCTCCTGGCA CCATCGCTGA TCCCAAAGAA CTGGCCAGAC
CAAGGGAAGA TCCAGATCCA GAACCTGAGC GTGCGCTACG ACAGCTCCCT GAAGCCGGTG
CTGAAGCACG TCAATGCCCT CATCGCCCCT GGACAGAAGA TCGGGATCTG CGGCCGCACC
GGCAGTGGGA AGTCCTCCTT CTCTCTTGCC TTCTTCCGCA TGGTGGACAC GTTCGAAGGG
CACATCATCA TTGATGGCAT TGACATCGCC AAACTGCCGC TGCACACCCT GCGCTCACGC
CTCTCCATCA TCCTGCAGGA CCCCGTCCTC TTCAGCGGCA CCATCCGATT TAACCTGGAC
CCTGAGAGGA AGTGCTCAGA TAGCACACTG TGGGAGGCCC TGGAAATCGC CCAGCTGAAG
CTGGTGGTGA AGGCACTGCC AGGAGGCCTC GATGCCATCA TCACAGAAGG CGGGGAGAAT
TTCAGCCAGG GACAGAGGCA GCTGTTCTGC CTGGCCCGGG CCTTCGTGAG GAAGACCAGC
ATCTTCATCA TGGACGAGGC CACGGCTTCC ATTGACATGG CCACGGAAAA CATCCTCCAA
AAGGTGGTGA TGACAGCCTT CGCAGACCGC ACTGTGGTCA CCATCGCGCA TCGAGTGCAC
ACCATCCTGA GTGCAGACCT GGTGATCGTC CTGAAGCGGG GTGCCATCCT TGAGTTCGAT
AAGCCAGAGA AGCTGCTCAG CCGGAAGGAC AGCGTCTTCG CCTCCTTCGT CCGTGCAGAC
AAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20799
Clone ID Related Accession (Same CDS sequence) NM_001287174
Accession Version NM_001287174.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4749bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product ATP-binding cassette sub-family C member 8 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC124798.5, AF087138.1, R84665.1, BU950000.1, BM352492.1 and BM313666.1. This sequence is a reference standard in the RefSeqGene project. On Dec 3, 2013 this sequence version replaced gi:530395702. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
ATGCCCCTGG CCTTCTGCGG CAGCGAGAAC CACTCGGCCG CCTACCGGGT GGACCAGGGG 
GTCCTCAACA ACGGCTGCTT TGTGGACGCG CTCAACGTGG TGCCGCACGT CTTCCTACTC
TTCATCACCT TCCCCATCCT CTTCATTGGA TGGGGAAGTC AGAGCTCCAA GGTGCACATC
CACCACAGCA CATGGCTTCA TTTCCCTGGG CACAACCTGC GGTGGATCCT GACCTTCATG
CTGCTCTTCG TCCTGGTGTG TGAGATTGCA GAGGGCATCC TGTCTGATGG GGTGACCGAA
TCCCACCATC TGCACCTGTA CATGCCAGCC GGGATGGCGT TCATGGCTGC TGTCACCTCC
GTGGTCTACT ATCACAACAT CGAGACTTCC AACTTCCCCA AGCTGCTAAT TGCCCTGCTG
GTGTATTGGA CCCTGGCCTT CATCACCAAG ACCATCAAGT TTGTCAAGTT CTTGGACCAC
GCCATCGGCT TCTCGCAGCT ACGCTTCTGC CTCACAGGGC TGCTGGTGAT CCTCTATGGG
ATGCTGCTCC TCGTGGAGGT CAATGTCATC AGGGTGAGGA GATACATCTT CTTCAAGACA
CCGAGGGAGG TGAAGCCTCC CGAGGACCTG CAAGACCTGG GGGTACGCTT CCTGCAGCCC
TTCGTGAATC TGCTGTCCAA AGGCACCTAC TGGTGGATGA ACGCCTTCAT CAAGACTGCC
CACAAGAAGC CCATCGACTT GCGAGCCATC GGGAAGCTGC CCATCGCCAT GAGGGCCCTC
ACCAACTACC AACGGCTCTG CGAGGCCTTT GACGCCCAGG TGCGGAAGGA CATTCAGGGC
ACTCAAGGTG CCCGGGCCAT CTGGCAGGCA CTCAGCCATG CCTTCGGGAG GCGCCTGGTC
CTCAGCAGCA CTTTCCGCAT CTTGGCCGAC CTGCTGGGCT TCGCCGGGCC ACTGTGCATC
TTTGGGATCG TGGACCACCT TGGGAAGGAG AACGACGTCT TCCAGCCCAA GACACAATTT
CTCGGGGTTT ACTTTGTCTC ATCCCAAGAG TTCCTTGCCA ATGCCTACGT CTTAGCTGTG
CTTCTGTTCC TTGCCCTCCT ACTGCAAAGG ACATTTCTGC AAGCATCCTA CTATGTGGCC
ATTGAAACTG GAATTAACTT GAGAGGAGCA ATACAGACCA AGATTTACAA TAAAATTATG
CACCTGTCCA CCTCCAACCT GTCCATGGGA GAAATGACTG CTGGACAGAT CTGTAATCTG
GTTGCCATCG ACACCAATCA GCTCATGTGG TTTTTCTTCT TGTGCCCAAA CCTCTGGGCT
ATGCCAGTAC AGATCATTGT GGGTGTGATT CTCCTCTACT ACATACTCGG AGTCAGTGCC
TTAATTGGAG CAGCTGTCAT CATTCTACTG GCTCCTGTCC AGTACTTCGT GGCCACCAAG
CTGTCTCAGG CCCAGCGGAG CACACTGGAG TATTCCAATG AGCGGCTGAA GCAGACCAAC
GAGATGCTCC GCGGCATCAA GCTGCTGAAG CTGTACGCCT GGGAGAACAT CTTCCGCACG
CGGGTGGAGA CGACCCGCAG GAAGGAGATG ACCAGCCTCA GGGCCTTTGC CATCTATACC
TCCATCTCCA TTTTCATGAA CACGGCCATC CCCATTGCAG CTGTCCTCAT AACTTTCGTG
GGCCACGTCA GCTTCTTCAA AGAGGCCGAC TTCTCGCCCT CCGTGGCCTT TGCCTCCCTC
TCCCTCTTCC ATATCTTGGT CACACCGCTG TTCCTGCTGT CCAGTGTGGT CCGATCTACC
GTCAAAGCTC TAGTGAGCGT GCAAAAGCTA AGCGAGTTCC TGTCCAGTGC AGAGATCCGT
GAGGAGCAGT GTGCCCCCCA TGAGCCCACA CCTCAGGGCC CAGCCAGCAA GTACCAGGCG
GTGCCCCTCA GGGTTGTGAA CCGCAAGCGT CCAGCCCGGG AGGATTGTCG GGGCCTCACC
GGCCCACTGC AGAGCCTGGT CCCCAGTGCA GATGGCGATG CTGACAACTG CTGTGTCCAG
ATCATGGGAG GCTACTTCAC GTGGACCCCA GATGGAATCC CCACACTGTC CAACATCACC
ATTCGTATCC CCCGAGGCCA GCTGACTATG ATCGTGGGGC AGGTGGGCTG CGGCAAGTCC
TCGCTCCTTC TAGCCGCACT GGGGGAGATG CAGAAGGTCT CAGGGGCTGT CTTCTGGAGC
AGCAGCCTTC CTGACAGCGA GATAGGAGAG GACCCCAGCC CAGAGCGGGA GACAGCGACC
GACTTGGATA TCAGGAAGAG AGGCCCCGTG GCCTATGCTT CGCAGAAACC ATGGCTGCTA
AATGCCACTG TGGAGGAGAA CATCATCTTT GAGAGTCCCT TCAACAAACA ACGGTACAAG
ATGGTCATTG AAGCCTGCTC TCTGCAGCCA GACATCGACA TCCTGCCCCA TGGAGACCAG
ACCCAGATTG GGGAACGGGG CATCAACCTG TCTGGTGGTC AACGCCAGCG AATCAGTGTG
GCCCGAGCCC TCTACCAGCA CGCCAACGTT GTCTTCTTGG ATGACCCCTT CTCAGCTCTG
GATATCCATC TGAGTGACCA CTTAATGCAG GCCGGCATCC TTGAGCTGCT CCGGGACGAC
AAGAGGACAG TGGTCTTAGT GACCCACAAG CTACAGTACC TGCCCCATGC AGACTGGATC
ATTGCCATGA AGGATGGCAC CATCCAGAGG GAGGGTACCC TCAAGGACTT CCAGAGGTCT
GAATGCCAGC TCTTTGAGCA CTGGAAGACC CTCATGAACC GACAGGACCA AGAGCTGGAG
AAGGAGACTG TCACAGAGAG AAAAGCCACA GAGCCACCCC AGGGCCTATC TCGTGCCATG
TCCTCGAGGG ATGGCCTTCT GCAGGATGAG GAAGAGGAGG AAGAGGAGGC AGCTGAGAGC
GAGGAGGATG ACAACCTGTC GTCCATGCTG CACCAGCGTG CTGAGATCCC ATGGCGAGCC
TGCGCCAAGT ACCTGTCCTC CGCCGGCATC CTGCTCCTGT CGTTGCTGGT CTTCTCACAG
CTGCTCAAGC ACATGGTCCT GGTGGCCATC GACTACTGGC TGGCCAAGTG GACCGACAGC
GCCCTGACCC TGACCCCTGC AGCCAGGAAC TGCTCCCTCA GCCAGGAGTG CACCCTCGAC
CAGACTGTCT ATGCCATGGT GTTCACGGTG CTCTGCAGCC TGGGCATTGT GCTGTGCCTC
GTCACGTCTG TCACTGTGGA GTGGACAGGG CTGAAGGTGG CCAAGAGACT GCACCGCAGC
CTGCTAAACC GGATCATCCT AGCCCCCATG AGGTTTTTTG AGACCACGCC CCTTGGGAGC
ATCCTGAACA GATTTTCATC TGACTGTAAC ACCATCGACC AGCACATCCC ATCCACGCTG
GAGTGCCTGA GCCGCTCCAC CCTGCTCTGT GTCTCAGCCC TGGCCGTCAT CTCCTATGTC
ACACCTGTGT TCCTCGTGGC CCTCTTGCCC CTGGCCATCG TGTGCTACTT CATCCAGAAG
TACTTCCGGG TGGCGTCCAG GGACCTGCAG CAGCTGGATG ACACCACCCA GCTTCCACTT
CTCTCACACT TTGCCGAAAC CGTAGAAGGA CTCACCACCA TCCGGGCCTT CAGGTATGAG
GCCCGGTTCC AGCAGAAGCT TCTCGAATAC ACAGACTCCA ACAACATTGC TTCCCTCTTC
CTCACAGCTG CCAACAGATG GCTGGAAGTC CGAATGGAGT ACATCGGTGC ATGTGTGGTG
CTCATCGCAG CGGTGACCTC CATCTCCAAC TCCCTGCACA GGGAGCTCTC TGCTGGCCTG
GTGGGCCTGG GCCTTACCTA CGCCCTAATG GTCTCCAACT ACCTCAACTG GATGGTGAGG
AACCTGGCAG ACATGGAGCT CCAGCTGGGG GCTGTGAAGC GCATCCATGG GCTCCTGAAA
ACCGAGGCAG AGAGCTACGA GGGGCTCCTG GCACCATCGC TGATCCCAAA GAACTGGCCA
GACCAAGGGA AGATCCAGAT CCAGAACCTG AGCGTGCGCT ACGACAGCTC CCTGAAGCCG
GTGCTGAAGC ACGTCAATGC CCTCATCGCC CCTGGACAGA AGATCGGGAT CTGCGGCCGC
ACCGGCAGTG GGAAGTCCTC CTTCTCTCTT GCCTTCTTCC GCATGGTGGA CACGTTCGAA
GGGCACATCA TCATTGATGG CATTGACATC GCCAAACTGC CGCTGCACAC CCTGCGCTCA
CGCCTCTCCA TCATCCTGCA GGACCCCGTC CTCTTCAGCG GCACCATCCG ATTTAACCTG
GACCCTGAGA GGAAGTGCTC AGATAGCACA CTGTGGGAGG CCCTGGAAAT CGCCCAGCTG
AAGCTGGTGG TGAAGGCACT GCCAGGAGGC CTCGATGCCA TCATCACAGA AGGCGGGGAG
AATTTCAGCC AGGGACAGAG GCAGCTGTTC TGCCTGGCCC GGGCCTTCGT GAGGAAGACC
AGCATCTTCA TCATGGACGA GGCCACGGCT TCCATTGACA TGGCCACGGA AAACATCCTC
CAAAAGGTGG TGATGACAGC CTTCGCAGAC CGCACTGTGG TCACCATCGC GCATCGAGTG
CACACCATCC TGAGTGCAGA CCTGGTGATC GTCCTGAAGC GGGGTGCCAT CCTTGAGTTC
GATAAGCCAG AGAAGCTGCT CAGCCGGAAG GACAGCGTCT TCGCCTCCTT CGTCCGTGCA
GACAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001274103.1
CDS70..4818
Misc Feature(1)172..234(+)
Misc Feature(2)295..357(+)
Misc Feature(3)373..435(+)
Misc Feature(4)472..531(+)
Misc Feature(5)571..651(+)
Misc Feature(6)736..4815(+)
Misc Feature(7)1003..1062(+)
Misc Feature(8)1021..1839(+)
Misc Feature(9)1135..1197(+)
Misc Feature(10)1372..1434(+)
Misc Feature(11)1444..1506(+)
Misc Feature(12)1693..1755(+)
Misc Feature(13)1822..1884(+)
Misc Feature(14)2104..2790(+)
Misc Feature(15)2206..2229(+)
Misc Feature(16)2215..2736(+)
Misc Feature(17)2383..2394(+)
Misc Feature(18)2557..2586(+)
Misc Feature(19)2617..2634(+)
Misc Feature(20)2641..2652(+)
Misc Feature(21)2722..2742(+)
Misc Feature(22)3085..3147(+)
Misc Feature(23)3106..3954(+)
Misc Feature(24)3289..3351(+)
Misc Feature(25)3484..3546(+)
Misc Feature(26)3550..3612(+)
Misc Feature(27)3826..3888(+)
Misc Feature(28)3901..3963(+)
Misc Feature(29)4096..4815(+)
Misc Feature(30)4204..4227(+)
Misc Feature(31)4213..4692(+)
Misc Feature(32)4339..4350(+)
Misc Feature(33)4513..4542(+)
Misc Feature(34)4573..4590(+)
Misc Feature(35)4597..4608(+)
Misc Feature(36)4678..4698(+)
Exon (1)1..217
Gene:ABCC8
Gene Synonym:
Exon (2)218..359
Gene:ABCC8
Gene Synonym:
Exon (3)360..481
Gene:ABCC8
Gene Synonym:
Exon (4)482..648
Gene:ABCC8
Gene Synonym:
Exon (5)649..891
Gene:ABCC8
Gene Synonym:
Exon (6)892..1080
Gene:ABCC8
Gene Synonym:
Exon (7)1081..1245
Gene:ABCC8
Gene Synonym:
Exon (8)1246..1401
Gene:ABCC8
Gene Synonym:
Exon (9)1402..1536
Gene:ABCC8
Gene Synonym:
Exon (10)1537..1699
Gene:ABCC8
Gene Synonym:
Exon (11)1700..1740
Gene:ABCC8
Gene Synonym:
Exon (12)1741..1886
Gene:ABCC8
Gene Synonym:
Exon (13)1887..1992
Gene:ABCC8
Gene Synonym:
Exon (14)1993..2109
Gene:ABCC8
Gene Synonym:
Exon (15)2110..2185
Gene:ABCC8
Gene Synonym:
Exon (16)2186..2291
Gene:ABCC8
Gene Synonym:
Exon (17)2292..2327
Gene:ABCC8
Gene Synonym:
Exon (18)2328..2363
Gene:ABCC8
Gene Synonym:
Exon (19)2364..2462
Gene:ABCC8
Gene Synonym:
Exon (20)2463..2547
Gene:ABCC8
Gene Synonym:
Exon (21)2548..2628
Gene:ABCC8
Gene Synonym:
Exon (22)2629..2766
Gene:ABCC8
Gene Synonym:
Exon (23)2767..2892
Gene:ABCC8
Gene Synonym:
Exon (24)2893..2992
Gene:ABCC8
Gene Synonym:
Exon (25)2993..3234
Gene:ABCC8
Gene Synonym:
Exon (26)3235..3401
Gene:ABCC8
Gene Synonym:
Exon (27)3402..3471
Gene:ABCC8
Gene Synonym:
Exon (28)3472..3629
Gene:ABCC8
Gene Synonym:
Exon (29)3630..3722
Gene:ABCC8
Gene Synonym:
Exon (30)3723..3825
Gene:ABCC8
Gene Synonym:
Exon (31)3826..3939
Gene:ABCC8
Gene Synonym:
Exon (32)3940..4060
Gene:ABCC8
Gene Synonym:
Exon (33)4061..4191
Gene:ABCC8
Gene Synonym:
Exon (34)4192..4270
Gene:ABCC8
Gene Synonym:
Exon (35)4271..4379
Gene:ABCC8
Gene Synonym:
Exon (36)4380..4483
Gene:ABCC8
Gene Synonym:
Exon (37)4484..4617
Gene:ABCC8
Gene Synonym:
Exon (38)4618..4680
Gene:ABCC8
Gene Synonym:
Exon (39)4681..4924
Gene:ABCC8
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001287174
Organism Homo sapiens (human)
Definition Homo sapiens ATP-binding cassette, sub-family C (CFTR/MRP), member 8 (ABCC8), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001287174

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
ATGCCCCTGG CCTTCTGCGG CAGCGAGAAC CACTCGGCCG CCTACCGGGT GGACCAGGGG 
GTCCTCAACA ACGGCTGCTT TGTGGACGCG CTCAACGTGG TGCCGCACGT CTTCCTACTC
TTCATCACCT TCCCCATCCT CTTCATTGGA TGGGGAAGTC AGAGCTCCAA GGTGCACATC
CACCACAGCA CATGGCTTCA TTTCCCTGGG CACAACCTGC GGTGGATCCT GACCTTCATG
CTGCTCTTCG TCCTGGTGTG TGAGATTGCA GAGGGCATCC TGTCTGATGG GGTGACCGAA
TCCCACCATC TGCACCTGTA CATGCCAGCC GGGATGGCGT TCATGGCTGC TGTCACCTCC
GTGGTCTACT ATCACAACAT CGAGACTTCC AACTTCCCCA AGCTGCTAAT TGCCCTGCTG
GTGTATTGGA CCCTGGCCTT CATCACCAAG ACCATCAAGT TTGTCAAGTT CTTGGACCAC
GCCATCGGCT TCTCGCAGCT ACGCTTCTGC CTCACAGGGC TGCTGGTGAT CCTCTATGGG
ATGCTGCTCC TCGTGGAGGT CAATGTCATC AGGGTGAGGA GATACATCTT CTTCAAGACA
CCGAGGGAGG TGAAGCCTCC CGAGGACCTG CAAGACCTGG GGGTACGCTT CCTGCAGCCC
TTCGTGAATC TGCTGTCCAA AGGCACCTAC TGGTGGATGA ACGCCTTCAT CAAGACTGCC
CACAAGAAGC CCATCGACTT GCGAGCCATC GGGAAGCTGC CCATCGCCAT GAGGGCCCTC
ACCAACTACC AACGGCTCTG CGAGGCCTTT GACGCCCAGG TGCGGAAGGA CATTCAGGGC
ACTCAAGGTG CCCGGGCCAT CTGGCAGGCA CTCAGCCATG CCTTCGGGAG GCGCCTGGTC
CTCAGCAGCA CTTTCCGCAT CTTGGCCGAC CTGCTGGGCT TCGCCGGGCC ACTGTGCATC
TTTGGGATCG TGGACCACCT TGGGAAGGAG AACGACGTCT TCCAGCCCAA GACACAATTT
CTCGGGGTTT ACTTTGTCTC ATCCCAAGAG TTCCTTGCCA ATGCCTACGT CTTAGCTGTG
CTTCTGTTCC TTGCCCTCCT ACTGCAAAGG ACATTTCTGC AAGCATCCTA CTATGTGGCC
ATTGAAACTG GAATTAACTT GAGAGGAGCA ATACAGACCA AGATTTACAA TAAAATTATG
CACCTGTCCA CCTCCAACCT GTCCATGGGA GAAATGACTG CTGGACAGAT CTGTAATCTG
GTTGCCATCG ACACCAATCA GCTCATGTGG TTTTTCTTCT TGTGCCCAAA CCTCTGGGCT
ATGCCAGTAC AGATCATTGT GGGTGTGATT CTCCTCTACT ACATACTCGG AGTCAGTGCC
TTAATTGGAG CAGCTGTCAT CATTCTACTG GCTCCTGTCC AGTACTTCGT GGCCACCAAG
CTGTCTCAGG CCCAGCGGAG CACACTGGAG TATTCCAATG AGCGGCTGAA GCAGACCAAC
GAGATGCTCC GCGGCATCAA GCTGCTGAAG CTGTACGCCT GGGAGAACAT CTTCCGCACG
CGGGTGGAGA CGACCCGCAG GAAGGAGATG ACCAGCCTCA GGGCCTTTGC CATCTATACC
TCCATCTCCA TTTTCATGAA CACGGCCATC CCCATTGCAG CTGTCCTCAT AACTTTCGTG
GGCCACGTCA GCTTCTTCAA AGAGGCCGAC TTCTCGCCCT CCGTGGCCTT TGCCTCCCTC
TCCCTCTTCC ATATCTTGGT CACACCGCTG TTCCTGCTGT CCAGTGTGGT CCGATCTACC
GTCAAAGCTC TAGTGAGCGT GCAAAAGCTA AGCGAGTTCC TGTCCAGTGC AGAGATCCGT
GAGGAGCAGT GTGCCCCCCA TGAGCCCACA CCTCAGGGCC CAGCCAGCAA GTACCAGGCG
GTGCCCCTCA GGGTTGTGAA CCGCAAGCGT CCAGCCCGGG AGGATTGTCG GGGCCTCACC
GGCCCACTGC AGAGCCTGGT CCCCAGTGCA GATGGCGATG CTGACAACTG CTGTGTCCAG
ATCATGGGAG GCTACTTCAC GTGGACCCCA GATGGAATCC CCACACTGTC CAACATCACC
ATTCGTATCC CCCGAGGCCA GCTGACTATG ATCGTGGGGC AGGTGGGCTG CGGCAAGTCC
TCGCTCCTTC TAGCCGCACT GGGGGAGATG CAGAAGGTCT CAGGGGCTGT CTTCTGGAGC
AGCAGCCTTC CTGACAGCGA GATAGGAGAG GACCCCAGCC CAGAGCGGGA GACAGCGACC
GACTTGGATA TCAGGAAGAG AGGCCCCGTG GCCTATGCTT CGCAGAAACC ATGGCTGCTA
AATGCCACTG TGGAGGAGAA CATCATCTTT GAGAGTCCCT TCAACAAACA ACGGTACAAG
ATGGTCATTG AAGCCTGCTC TCTGCAGCCA GACATCGACA TCCTGCCCCA TGGAGACCAG
ACCCAGATTG GGGAACGGGG CATCAACCTG TCTGGTGGTC AACGCCAGCG AATCAGTGTG
GCCCGAGCCC TCTACCAGCA CGCCAACGTT GTCTTCTTGG ATGACCCCTT CTCAGCTCTG
GATATCCATC TGAGTGACCA CTTAATGCAG GCCGGCATCC TTGAGCTGCT CCGGGACGAC
AAGAGGACAG TGGTCTTAGT GACCCACAAG CTACAGTACC TGCCCCATGC AGACTGGATC
ATTGCCATGA AGGATGGCAC CATCCAGAGG GAGGGTACCC TCAAGGACTT CCAGAGGTCT
GAATGCCAGC TCTTTGAGCA CTGGAAGACC CTCATGAACC GACAGGACCA AGAGCTGGAG
AAGGAGACTG TCACAGAGAG AAAAGCCACA GAGCCACCCC AGGGCCTATC TCGTGCCATG
TCCTCGAGGG ATGGCCTTCT GCAGGATGAG GAAGAGGAGG AAGAGGAGGC AGCTGAGAGC
GAGGAGGATG ACAACCTGTC GTCCATGCTG CACCAGCGTG CTGAGATCCC ATGGCGAGCC
TGCGCCAAGT ACCTGTCCTC CGCCGGCATC CTGCTCCTGT CGTTGCTGGT CTTCTCACAG
CTGCTCAAGC ACATGGTCCT GGTGGCCATC GACTACTGGC TGGCCAAGTG GACCGACAGC
GCCCTGACCC TGACCCCTGC AGCCAGGAAC TGCTCCCTCA GCCAGGAGTG CACCCTCGAC
CAGACTGTCT ATGCCATGGT GTTCACGGTG CTCTGCAGCC TGGGCATTGT GCTGTGCCTC
GTCACGTCTG TCACTGTGGA GTGGACAGGG CTGAAGGTGG CCAAGAGACT GCACCGCAGC
CTGCTAAACC GGATCATCCT AGCCCCCATG AGGTTTTTTG AGACCACGCC CCTTGGGAGC
ATCCTGAACA GATTTTCATC TGACTGTAAC ACCATCGACC AGCACATCCC ATCCACGCTG
GAGTGCCTGA GCCGCTCCAC CCTGCTCTGT GTCTCAGCCC TGGCCGTCAT CTCCTATGTC
ACACCTGTGT TCCTCGTGGC CCTCTTGCCC CTGGCCATCG TGTGCTACTT CATCCAGAAG
TACTTCCGGG TGGCGTCCAG GGACCTGCAG CAGCTGGATG ACACCACCCA GCTTCCACTT
CTCTCACACT TTGCCGAAAC CGTAGAAGGA CTCACCACCA TCCGGGCCTT CAGGTATGAG
GCCCGGTTCC AGCAGAAGCT TCTCGAATAC ACAGACTCCA ACAACATTGC TTCCCTCTTC
CTCACAGCTG CCAACAGATG GCTGGAAGTC CGAATGGAGT ACATCGGTGC ATGTGTGGTG
CTCATCGCAG CGGTGACCTC CATCTCCAAC TCCCTGCACA GGGAGCTCTC TGCTGGCCTG
GTGGGCCTGG GCCTTACCTA CGCCCTAATG GTCTCCAACT ACCTCAACTG GATGGTGAGG
AACCTGGCAG ACATGGAGCT CCAGCTGGGG GCTGTGAAGC GCATCCATGG GCTCCTGAAA
ACCGAGGCAG AGAGCTACGA GGGGCTCCTG GCACCATCGC TGATCCCAAA GAACTGGCCA
GACCAAGGGA AGATCCAGAT CCAGAACCTG AGCGTGCGCT ACGACAGCTC CCTGAAGCCG
GTGCTGAAGC ACGTCAATGC CCTCATCGCC CCTGGACAGA AGATCGGGAT CTGCGGCCGC
ACCGGCAGTG GGAAGTCCTC CTTCTCTCTT GCCTTCTTCC GCATGGTGGA CACGTTCGAA
GGGCACATCA TCATTGATGG CATTGACATC GCCAAACTGC CGCTGCACAC CCTGCGCTCA
CGCCTCTCCA TCATCCTGCA GGACCCCGTC CTCTTCAGCG GCACCATCCG ATTTAACCTG
GACCCTGAGA GGAAGTGCTC AGATAGCACA CTGTGGGAGG CCCTGGAAAT CGCCCAGCTG
AAGCTGGTGG TGAAGGCACT GCCAGGAGGC CTCGATGCCA TCATCACAGA AGGCGGGGAG
AATTTCAGCC AGGGACAGAG GCAGCTGTTC TGCCTGGCCC GGGCCTTCGT GAGGAAGACC
AGCATCTTCA TCATGGACGA GGCCACGGCT TCCATTGACA TGGCCACGGA AAACATCCTC
CAAAAGGTGG TGATGACAGC CTTCGCAGAC CGCACTGTGG TCACCATCGC GCATCGAGTG
CACACCATCC TGAGTGCAGA CCTGGTGATC GTCCTGAAGC GGGGTGCCAT CCTTGAGTTC
GATAAGCCAG AGAAGCTGCT CAGCCGGAAG GACAGCGTCT TCGCCTCCTT CGTCCGTGCA
GACAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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